LOCUS       DAWGJV010000001        49902 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWGJV010000001 DAWGJV010000000
VERSION     DAWGJV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.001"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(38..598)
                     /locus_tag="WHQ94_000001"
     CDS             complement(38..598)
                     /locus_tag="WHQ94_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127358.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(516..703)
                     /locus_tag="WHQ94_000002"
                     /pseudo
     CDS             complement(516..703)
                     /locus_tag="WHQ94_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(711..1103)
                     /locus_tag="WHQ94_000003"
     CDS             complement(711..1103)
                     /locus_tag="WHQ94_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2127359.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(1311..1703)
                     /locus_tag="WHQ94_000004"
     CDS             complement(1311..1703)
                     /locus_tag="WHQ94_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2127360.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(1820..2230)
                     /locus_tag="WHQ94_000005"
     CDS             complement(1820..2230)
                     /locus_tag="WHQ94_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2127361.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(2244..2681)
                     /locus_tag="WHQ94_000006"
     CDS             complement(2244..2681)
                     /locus_tag="WHQ94_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2127362.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(3092..4390)
                     /locus_tag="WHQ94_000007"
     CDS             complement(3092..4390)
                     /locus_tag="WHQ94_000007"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ2127363.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(4494..5645)
                     /locus_tag="WHQ94_000008"
     CDS             complement(4494..5645)
                     /locus_tag="WHQ94_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ2127364.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRVRPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5980..7359
                     /gene="norM"
                     /locus_tag="WHQ94_000009"
     CDS             5980..7359
                     /gene="norM"
                     /locus_tag="WHQ94_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ2127365.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSIYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            7538..8578
                     /gene="murB"
                     /locus_tag="WHQ94_000010"
     CDS             7538..8578
                     /gene="murB"
                     /locus_tag="WHQ94_000010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ2127366.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYSGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTEAFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKEHFVPNLGYGDLAAAVAERCAGREITAKEVS
                     DAVCAVRRRKLPDPGILGNVGSFFKNPVVGAGKAAALLQQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFAQFQVELH
                     AEPNWLPASFSL"
     gene            8590..9240
                     /locus_tag="WHQ94_000011"
     CDS             8590..9240
                     /locus_tag="WHQ94_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ2127367.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREEYDRKIGNGNP"
     gene            9295..10071
                     /locus_tag="WHQ94_000012"
     CDS             9295..10071
                     /locus_tag="WHQ94_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2127368.1"
                     /translation="MCAVPPPHISITGLGYLGLPLAQKFYQHSSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            10122..10688
                     /locus_tag="WHQ94_000013"
     CDS             10122..10688
                     /locus_tag="WHQ94_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ2127369.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            10708..11598
                     /locus_tag="WHQ94_000014"
     CDS             10708..11598
                     /locus_tag="WHQ94_000014"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ2127370.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            11623..12381
                     /locus_tag="WHQ94_000015"
     CDS             11623..12381
                     /locus_tag="WHQ94_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ2127371.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(12436..13101)
                     /locus_tag="WHQ94_000016"
     CDS             complement(12436..13101)
                     /locus_tag="WHQ94_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ2127372.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(13301..14197)
                     /locus_tag="WHQ94_000017"
     CDS             complement(13301..14197)
                     /locus_tag="WHQ94_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ2127373.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            14266..15423
                     /locus_tag="WHQ94_000018"
     CDS             14266..15423
                     /locus_tag="WHQ94_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ2127374.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="WHQ94_000019"
     CDS             complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="WHQ94_000019"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ2127375.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="WHQ94_000020"
     CDS             complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="WHQ94_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ2127376.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            17064..17684
                     /locus_tag="WHQ94_000021"
     CDS             17064..17684
                     /locus_tag="WHQ94_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ2127377.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            17748..19715
                     /gene="ftsH"
                     /locus_tag="WHQ94_000022"
     CDS             17748..19715
                     /gene="ftsH"
                     /locus_tag="WHQ94_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ2127378.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            19937..20368
                     /locus_tag="WHQ94_000023"
     CDS             19937..20368
                     /locus_tag="WHQ94_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ2127379.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            20361..20639
                     /locus_tag="WHQ94_000024"
     CDS             20361..20639
                     /locus_tag="WHQ94_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ2127380.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            20692..21270
                     /gene="pth"
                     /locus_tag="WHQ94_000025"
     CDS             20692..21270
                     /gene="pth"
                     /locus_tag="WHQ94_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ2127381.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDVVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            21331..21537
                     /locus_tag="WHQ94_000026"
     CDS             21331..21537
                     /locus_tag="WHQ94_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ2127382.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            21534..21896
                     /locus_tag="WHQ94_000027"
     CDS             21534..21896
                     /locus_tag="WHQ94_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ2127383.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            22127..23542
                     /locus_tag="WHQ94_000028"
     CDS             22127..23542
                     /locus_tag="WHQ94_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ2127384.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            23661..24170
                     /locus_tag="WHQ94_000029"
     CDS             23661..24170
                     /locus_tag="WHQ94_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2127385.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            24333..25715
                     /locus_tag="WHQ94_000030"
     CDS             24333..25715
                     /locus_tag="WHQ94_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ2127386.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(25735..26490)
                     /locus_tag="WHQ94_000031"
     CDS             complement(25735..26490)
                     /locus_tag="WHQ94_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ2127387.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(26500..27216)
                     /locus_tag="WHQ94_000032"
     CDS             complement(26500..27216)
                     /locus_tag="WHQ94_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ2127388.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGSIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(27206..28033)
                     /locus_tag="WHQ94_000033"
     CDS             complement(27206..28033)
                     /locus_tag="WHQ94_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2127389.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            28318..29226
                     /locus_tag="WHQ94_000034"
     CDS             28318..29226
                     /locus_tag="WHQ94_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ2127390.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKSACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            29306..32920
                     /gene="recB"
                     /locus_tag="WHQ94_000035"
     CDS             29306..32920
                     /gene="recB"
                     /locus_tag="WHQ94_000035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ2127391.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAALKKGKTPDAAAFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWKTALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAGDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            32959..33318
                     /locus_tag="WHQ94_000036"
     CDS             32959..33318
                     /locus_tag="WHQ94_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ2127392.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            33560..34039
                     /locus_tag="WHQ94_000037"
     CDS             33560..34039
                     /locus_tag="WHQ94_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ2127393.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMNIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(34110..35489)
                     /gene="radA"
                     /locus_tag="WHQ94_000038"
     CDS             complement(34110..35489)
                     /gene="radA"
                     /locus_tag="WHQ94_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ2127394.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(35678..36742)
                     /locus_tag="WHQ94_000039"
     CDS             complement(35678..36742)
                     /locus_tag="WHQ94_000039"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ2127395.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDEAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(36817..38034)
                     /locus_tag="WHQ94_000040"
     CDS             complement(36817..38034)
                     /locus_tag="WHQ94_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ2127396.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPQSRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            complement(38031..39365)
                     /locus_tag="WHQ94_000041"
     CDS             complement(38031..39365)
                     /locus_tag="WHQ94_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ2127397.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLLSPEQAYFVRENLRLRLLDARIALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQSVPEKAGRSPSAKGERA"
     gene            complement(39380..40141)
                     /locus_tag="WHQ94_000042"
     CDS             complement(39380..40141)
                     /locus_tag="WHQ94_000042"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ2127398.1"
                     /translation="METAKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKIHIAVGQGSRRALERYLGRTV
                     IAPHDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(40159..40581)
                     /locus_tag="WHQ94_000043"
     CDS             complement(40159..40581)
                     /locus_tag="WHQ94_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127399.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLSGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(40832..41152)
                     /locus_tag="WHQ94_000044"
     CDS             complement(40832..41152)
                     /locus_tag="WHQ94_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ2127400.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(41175..41801)
                     /gene="upp"
                     /locus_tag="WHQ94_000045"
     CDS             complement(41175..41801)
                     /gene="upp"
                     /locus_tag="WHQ94_000045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ2127401.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="WHQ94_000046"
     CDS             complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="WHQ94_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ2127402.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(42319..42534)
                     /locus_tag="WHQ94_000047"
     CDS             complement(42319..42534)
                     /locus_tag="WHQ94_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ2127403.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(42560..43339)
                     /locus_tag="WHQ94_000048"
     CDS             complement(42560..43339)
                     /locus_tag="WHQ94_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ2127404.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQVLAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDTAFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(43344..43694)
                     /locus_tag="WHQ94_000049"
     CDS             complement(43344..43694)
                     /locus_tag="WHQ94_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ2127405.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(43698..44675)
                     /locus_tag="WHQ94_000050"
     CDS             complement(43698..44675)
                     /locus_tag="WHQ94_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ2127406.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHGEALAYLRDRGVAEPEEFLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(44709..45821)
                     /locus_tag="WHQ94_000051"
     CDS             complement(44709..45821)
                     /locus_tag="WHQ94_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ2127407.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            46007..46708
                     /locus_tag="WHQ94_000052"
     CDS             46007..46708
                     /locus_tag="WHQ94_000052"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ2127408.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            46851..48644
                     /gene="lepA"
                     /locus_tag="WHQ94_000053"
     CDS             46851..48644
                     /gene="lepA"
                     /locus_tag="WHQ94_000053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ2127409.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFISTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANSATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            48787..49806
                     /gene="lepB"
                     /locus_tag="WHQ94_000054"
     CDS             48787..49806
                     /gene="lepB"
                     /locus_tag="WHQ94_000054"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ2127410.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
BASE COUNT        11813 a        14208 c        12615 g        11266 t
ORIGIN      
        1 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcccctata tcagataaaa
       61 cagcggtaaa attgccgtct gaaaccaact ctgcgccata cgggacacgc tctgcacgtc
      121 tatttgcctc cgatatatcc tccctactgt aggaacgtct gccgatttgc acgaaatcgg
      181 cacctctagc acccttcagg atggcggacg ttatccgttc ttgggattct gcaagacggt
      241 cgacggcatt atacagacgc tcggtctgat acccttccga agcaacctgc ttcaggtatt
      301 ccaaataacc gtaacccaac gcccctccga caagcagggt cgtcaacgct accagcgttg
      361 ccgaacggat tttagggggc atatcttgaa gcatatctct accgaaccga accaaagtct
      421 ctttaaagtc ggactctaca cgcgtagaac cctcggaaat cttgaagttc aactcatact
      481 gcttccgttt gtccgctgtc aaccgcttga ggttgtcatc cccgtattgg acggttgaca
      541 caaccctgta cagactgtcc tgtatttcga ccagcgtctg tgcaaagttg gcggtcatac
      601 tggattccca acccttgccg ccgattctga tactcaaaac ggtatccagg cgttccagca
      661 gggcttcggg agacagcagt gcgtattctt caatattttc cataggttga ttatagcaga
      721 accttatacc tgttacagac aaggttgtcg ggatttgaac gtccgtcgag cttggttcgt
      781 atagagccgt agggggtgtt tagggctttg gcggcagctt gtacgctggg gaacgtgagt
      841 gtttcgcctg tggcggtgtc gaggatggag acaggttttt ggttttgggg aggcgtattt
      901 tccttcattt taagtttccc gcactccgga cacccatact ttgactttat agctgtgtcg
      961 taatacaaaa gttggacaac cccgtgcgta ggacatctaa cactggaagg tttataaatg
     1021 ccactgaact ccaacaattc taaatgtgga aatttctctt gaaagcgtga tttggcggta
     1081 tcgaaagtca ttcgcttacc cataatttgt ctccataggt tatgtgcaaa tggattacac
     1141 aaccaaaata tagaaatttt caaatttttt gagacctacc tggaatcctg cgcaagcaca
     1201 atgtaaagaa aaatggtata cgtctttact aaatctttac ataaattaac tctaatcggg
     1261 atttgaacgt ccgtcgagct tggttcgtag gatttagggt gttgtgaacc ttacctatcg
     1321 tacccaagca ctttgtacct gtttgaaata aggttgtcgg gcgacgttcg acccttgagc
     1381 ctgtgattga tttgttggaa atgtacacct aaagcagcac ctgcggcagt aacgcttggg
     1441 aacgtgagtg tttcgttggt cgttgtgtcg aggatgagga ggggcttccc tacatttgga
     1501 ggtgtttggg tttttgacat cttagcacac tcaggacacc cgtactttga ctttataagg
     1561 gcattaaatg tcgaataacg aacgttcccg tgcataggac aattaacggt tactgggtaa
     1621 ttcacaccgt caaagccggt tagagagata ttagggaata tatcagtaag ttcagaagct
     1681 gcttggttga aagatttggc catttgagac tccaaaattg attgtcaaat ggatttaata
     1741 gcaaatttat aaaaatgtca aatttttcga gaagcacctg atagtggcga ggggtataca
     1801 ctgttaaaaa gtgtatacgt catttagtgc agatgtgaac cttatacctc cctgcaagta
     1861 aagtctctgt gtgaacccga ccttttatac ggtcagtgat agctgctggg tttataccca
     1921 atgaccttgc agcagatgct cttgagggga atgtaagtgt ttcgccagtt acagtatcct
     1981 ccaatactgt atttttagat aatttatcta ttgtatcttc tggaattttg tgggaattga
     2041 caccataaga tgcacatttt ggacacccac ttttagagtc caacatactt ttgaatgtag
     2101 agcaagtaac cctcccgtgt ataggacaga cgattaccgt cgggtaacga acgccattga
     2161 actccacaag attcaaatgt ggaaatttag aggttagctt agaaactgct tgagtaaatg
     2221 taagtgccat agtggttgtg aacttacttt accaacctat aacgatacct aatcaatcgg
     2281 tcaaacggag tgcgtccgct taaacgtaca gctaaagtgc tgttggagca accgataaat
     2341 ttactggcag ccagtgtact tgcaaactcg tagatttccc ctgtctctgt gtcctccaac
     2401 ctaatggcaa cggcgtttgg agagggcttt tcgggatttc gagtacattc aggacatcct
     2461 gttgaggatt tcatggcagc ttgtagattc ccgtaaacaa ccgacccatg caaagggcat
     2521 tggattgtta ccggttcgta gtttttagtg aacttcaaaa ccgtcttatc aggaaattta
     2581 ctggaaaacc ttaactgaac atcttgtagc gagagtgatt ttggcattat agacctttct
     2641 aaaatgaaag cattatacaa ccaaaatata gaaattttca aattttctct gaacgcactg
     2701 gtatccgatg gagctaaatg taaagaaaag ggtatatgtc cttactaaat ctttacacat
     2761 tttaactgta tagtgtttga caagcaacag gcataggcgc ataatgcacc cgtagttaga
     2821 tagatagtaa ggagataacc aatgacttag cacctgcccc cctctctctc gcgcaccccg
     2881 cccctcgctt gcggctaatg ggctttgcgc gaaatataca ctttcatctc ctgcatggct
     2941 taaccgccta tgcaatagta cacacatcaa caaatcaggc tggcacgtct tggcattggc
     3001 tgacgtacca ttgtagcccg aaatgttgat accctactat ttatgaccaa cggcggatta
     3061 gggcttcaga cggcaatttt actgcaaaac cttatgcgaa cggatgatgc agcacaatcg
     3121 tttcttctcg gtcggggccg gtggagacga tggcgaccgg cgcgccgcag acttcttcaa
     3181 tccgtttcaa atatgctttg gcattatcgg gcagttcctc ataatttttg atgccgaaag
     3241 tcgattcttt ccaaccctcc attgtttcgt aaatcggttt gcaggtttcc accgcatccg
     3301 aaccgcaagg caggatatcg gttttgccgc cgtcgggcaa ttcgtagccg acgcagatat
     3361 tgatggtttc aacgccgtcc attacatcga gtttggtaat gcacataccg gaaatgccgt
     3421 tgatttggat ggagcgtttc agggcggcgg catcaaacca gccgcagcgg cgtgcgcggc
     3481 cggttaccga gccgaattcg tgtccgcgtt ccgccaaacc tgcgcctact tcgtcgaaca
     3541 attcggtcgg gaacgggcct gaaccgacgc gcgtggtata ggctttgacg atgcccaaaa
     3601 cataatccag catttgaggg cctacgcccg cgcctgcgga agcggctccc gccagacagt
     3661 tggacgaggt aacgaaggga taagtgccgt agtcgatgtc caacaacgca ccttgcgcgc
     3721 cttcaaacag cagtttttcg ccgtttttgt ttttctcgtt caacacgcgg gacacgtcgg
     3781 taatcatcgg cgcaatgcgc agcgcgactt tttcgataac cgccatcacg tcttccgctt
     3841 taaccggctc ggcattgtgc agatgttgca gttggacatt gtaataggca aggatggcat
     3901 ccagtttttc acgcagtttt tcaggatgca gcaaatcggc ggcgcgaatg gcgcggcgtg
     3961 ccactttgtc ttcgtaggca gggccgatgc cgcggccggt cgtgccgatt ttgcctttgc
     4021 cgcgcgatgc ttcgcgggct tggtcgagcg cgatgtggta aggcaggatc agcgggcagg
     4081 tcggcgcgat tttcagacgg ccttcgacgt ttttcacgcc tgcagcgttt aactcgtcga
     4141 tttcgcccaa cagggcttcg ggggagacga caacgcccga accgatgaag cagtccagac
     4201 tttcatgcag gatgccgctc ggaatcaggc gcaaaatggt ttttttgccg ccgacgacca
     4261 aagtatgacc cgcattgtgg ccgccttgga agcgcaccac gccgccggct tcttccgcca
     4321 gccagtcaac gattttacct ttaccctcgt cgccccactg tgcgccgatt actacaacat
     4381 ttttagccat agccatataa cctatcgata ttaaaaatta ttgcggaaat ccccaaaccg
     4441 gtgcggcttt gcgccccacc acgatttgcc tttcatccgc catgaacggg tatttagcgt
     4501 ttcacgacct gccaaacgcc gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca
     4561 ttgtgtccga taccgtagtc aatcacgaca cactgccctt gttcacgcaa ggcttcgact
     4621 gcttcgcgcg ccgcttcggc atcttccgca tcgaccaaca cggcgggctg ccgttcgctg
     4681 gtgggcaaac gcccgataaa gctgcgcaag tcgaaactga atcccgttgc cgggcgcacc
     4741 ctgccgaaat atccgcccaa tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa
     4801 tcggcggcat aggcggcata cagcaagccc gtgtggtaat tgtcgacacg cagctcggac
     4861 aagtcgatat ggatttcaca atcggggaat gcgtcgcaca ccgcctgcaa ttcgtctaac
     4921 gcgccgccga ccgccgacaa atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc
     4981 ccgccgtaca ggcgcggcag cagcgagaat gcttttgccc acatgccgtc cagcttccaa
     5041 gccttgacct gcgcttcgac cgtcccggta tctttatcct gcatcaaggc aagcagcgtt
     5101 gcagactgcc ccgcatccaa atgcgccgca tcggacaagg cgcgaaatat gccgatatgc
     5161 cccagcgaaa gcagcacttt gcccatatcg gcaattttca tgcttttcag catcaggtct
     5221 atcagctcga tgtcgccacg gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag
     5281 ggttcgcgca tattcagcag accgtcgggc tgcgcgtgca acaccggacc ggcataacac
     5341 aaccgattaa tcccttggtt ggcggataaa agatgggcat cgatacgcgc cacctgcggc
     5401 gtgatgtcgg cgcgtatgcc caactgcctg ccgctgagcc tgtccgttac caaaatggtt
     5461 ttcagggaaa gccccgcatc gatatgcgtc agcagggaat gtgcgtactc catcagcgga
     5521 ggctgtacca gttcataacc gtgtacgcgg aacagtgcca acaactgctc cctcgcgctt
     5581 tcaagctgcc gcgcgttcgt gggcagtacg tcggcgatat gttccggaag ctgccatgtc
     5641 tgcatgagaa tctgccttct attttatcat gtaaatacaa aaggctgccc gatccgcaat
     5701 cagacggcat ctttgccgca acgcatcacg ccgctgtcaa aacggcgatg cttccttccg
     5761 attcagaatc cgaacagccc ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa
     5821 ctttaccata aaatacacac aatctgaata tacttttata ccctgtatcc gccgtctgac
     5881 ggcacagcat ttttttacga tataatgtgc accattattt ctcatcactt cctgctgccg
     5941 ttttctgctt cagacggcat ttttgttgat tgaacactta tgctgctcga cctcaaccgc
     6001 ttttcctttt ccgtcttcct gaaagaagtc cgcctgctga ccgctcttgc cctgcccatg
     6061 ctgttggcgc aggtcgcgca ggtgggcatc ggttttgtcg ataccgtgat ggcgggcggt
     6121 gcgggcaagg aagacttggc ggcggtggct ttgggcagca gcgcgtttgc cacggtttat
     6181 attaccttta tgggcattat ggcggcgctg aacccgatga ttgcccagct ttacggcgcg
     6241 ggtaaaaccg acgaagtggg cgaaacggga cggcagggga tttggttcgg gctgtttttg
     6301 ggcgtgttcg gcatggtctt gatgtgggcg gcgattacgc cgttccgcaa ctggctgacc
     6361 ttgagcgatt atgtggaagg cacaatggcg cagtatatgc tgttcaccag cttggcgatg
     6421 ccggcggcaa tggtacaccg cgcactgcac gcctacgcct ccagcctgaa ccgcccgcgc
     6481 ctgattatgt tggtcagctt tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc
     6541 gtttacggca aattcggtat gcccgctttg ggcggcgcag gctgcggact ggcgacgatg
     6601 gcggtgtttt ggttcagcgc gctggcattg tggatttata tcgccaagga aaatttcttc
     6661 cgcccattcg gactgacggc gaaattcggc aaaccggatt gggcggtgtt caaacagatt
     6721 tggaaaatcg gcgcacccat cgggctgtct tattttttgg aagccagcgc gttttcgttt
     6781 atcgtgtttt tgattgcgcc tttcggcgag gattatgtgg cggcgcagca ggtcggcatc
     6841 agtttgtcgg ggattctcta tatgattccg caaagcgtcg gctcggcggg gacggtgcgc
     6901 atcggctttt cgcttgggcg gcgcgaattt tcgcgggcgc gttatatttc gggcgtgtca
     6961 ctggtgttag gatggatgct cgccgtgatt accgtgcttt ccttggtatt attccgttcg
     7021 ccgctggtaa gtatatacaa caatgatccg gcggttttaa gcatcgccgc caccgtctta
     7081 ctgttcgccg gcttgttcca accggcagac ttcacccaat gtatcgcctc ctacgccttg
     7141 cgcggctaca aagttacaaa ggtgccgatg ttcatccacg ccgccgcctt ttggggctgc
     7201 ggtctgctgc cgggctacct gctcgcctac cgtttcgata tgggcattta cggcttctgg
     7261 acggcattga ttgcctcgct caccatcgcc gccatcgcct tggtgtggtg cttggaattg
     7321 tgcagtaggg agatggtcag atcgcataag gctgtctgaa aactccgccg attaaaaata
     7381 tagtggatta acaaaaacca gtacggcgtt acctcgcctt gccgtactat ttgtactgtc
     7441 tgcggcttcg ttgccttgtc ctgatttttg ttaatccact atagaaacga tgggctgaag
     7501 cccgccactg gcaatactca ttttaaagtt caaacccatg caacccatcc gataccgaac
     7561 cgaccttacc ccctacaaca ctttcggcct gcgcgcccaa gcccgggcct ttatcgcgct
     7621 cgaacatgcc gacgagttgt gcgacatcgt ccgactgccc gagttcgacc gcgatactgt
     7681 tttatggctg ggcggcggca gcaacatcct tttgatgcag gattactccg gactggtcgt
     7741 acacatggaa aacaaaggca tacgcgagat tgcgcgttca gacggcattg ttcgcatcga
     7801 agcgcaggcg ggcgaaatct ggcacgattt tgtcctgcac accgtcgcgc tgggcttgaa
     7861 tgggctggaa aacctgagcc tgattccggg tacggtcggc gcgtcgcccg tgcagaacat
     7921 cggcgcatac ggcgtagagg cgaaagatgt gattcacagc gtgcgctgct ttgatttgga
     7981 tacggaggcc tttgtcgagc ttgccaatgc cgactgccgc ttcgcctacc gcgaaagcct
     8041 gttcaagcag gaaggtaaag ggcgttatgt gattgtttcg gttgtgtttg cattaaaaga
     8101 gcattttgtg ccgaacttgg gctacggcga tttggcggca gcggttgccg aacggtgcgc
     8161 cggcagggaa ataacggcga aagaagtttc cgatgcagtg tgcgccgtcc gccgccgcaa
     8221 actgcccgac ccgggcattt tgggcaatgt cggcagtttc tttaaaaacc ccgtcgtcgg
     8281 cgcgggaaaa gccgccgccc tgttgcagca gcatcccgat atgccgcatt atccacagcc
     8341 ggacggttcg gtcaaacttg ctgccggctg gctgatcgac caatgccgtc tgaaaggctt
     8401 ccaaatcggc ggcgcggcgg tacacgacaa acaggcattg gttttggtaa acaaaaacaa
     8461 cgcctcggca aacgatgtcc ggcaattggc gcaacacatc aaatttacag tatttgctca
     8521 gtttcaggta gaattgcacg ccgaacctaa ttggctgcct gcttcgttca gcctgtaaat
     8581 ccaaggagta tgcccgtgac ccgccccgcc aaaatcaata cttacacacg catcatcgac
     8641 gccagccttg cgctcttcaa cgaggaaggc gagcgcaaca tcagcaccaa ccatattgcc
     8701 gcccacttgg gcatcagtcc gggcaacctc tattaccact tccgcaacaa agacgaaatc
     8761 atcgtccaac tgttcaaacg ttacagcgaa gccctgcttg cctacctgaa tgaagctgtg
     8821 ttgccgtctg atgtggaaga ctccatcaac tatatggcgg gcatttacga cgtgatgtgg
     8881 gaataccgct tcctattcag cgacgtgaac accctgcttg cacgcagtgc cgaactatta
     8941 ggcgaacaca atactttcac ccaagccaaa gtctccccgc tcttggtcaa cctgctgacc
     9001 cagctcaacg gtctgaacat catccaagcc gaccaaaccg ctatgaacga tctcgccgtc
     9061 aatatgtgga tggttacgaa atactggttc gacttcgaca gctccctgcg cggccgcacc
     9121 aagctgaccg aagactccaa agcacgcggc atccgccgca ccttaagcct ccttcgtcct
     9181 tatcttttgc ccgaacaccg cgaggaatac gaccgcaaaa tcggcaacgg caacccgtaa
     9241 acccacagcg tccgaatcag ccttcagacg gcacttaccc gacggtatca gcgaatgtgc
     9301 gcagtccccc cgccccatat ctccatcaca ggtctaggtt acctcggcct gccgctggca
     9361 caaaagtttt accaacacag cagccgcgtc gccgccataa aacgcagcct gacttcggac
     9421 gacatcaacc tgcccataca cctcgacacc ttcgacctcg gcagcaccga tgcctttcag
     9481 acggcattgt ggcggcacca cgccgacaaa cccgtctggt tcttcctgct gccgccctcc
     9541 tcgctgacgc attacgccga caccgtcaaa caatgggcag aacttgcccg ggcgtgcaac
     9601 gtgcaacacc tgattttcac aagcagcacc agcgtttacg gcgatacagc gcgcgaatgc
     9661 gacgaaaccg ccacacccga tccgcaaacc gagtccgccc gccaaatcct cgccgccgaa
     9721 caacacctgc tcgacagcgg catctccaac atcgacatcc tgcggctggg cgggctttat
     9781 tgcgccgaac gccatcccgt cagccgcctt gttcaaaagc aaaacatccc gggcggcaac
     9841 cgccccatca acatcgtcca ccgtaatatc gccgtcgaaa gcctgtttca gacggcattt
     9901 aaccccggcg gcaggcggct gaaaaacatt atcgaaccgc gccacccgac acgacgcgaa
     9961 ttctacacgg aagaagccgc caaactcggc ctgcccgcgc cggatttcgc acccgacgac
    10021 agcgtgggca aaatcatccg taccgtttgc gataacggct taagcctgta aaataagcgg
    10081 caacagcaac aaacaggcat tctccgcccc aatcagaaaa tatgtccgcc ttcctcccca
    10141 tcatcaaccg cctgattctg caaagcccgg acagccgctc ggaacttgcc gcctttgcag
    10201 gcaaaacact gaccctgaac attgccgggc tgaaactggc gggacgcatc acggaagacg
    10261 gtttgctctc ggcgggaaac ggctttgcag acaccgaaat caccttccgc aacagcgcgg
    10321 tacagaaaat cctccaaggc ggcgaacccg gggcgggcga catcgggctc gaaggcgacc
    10381 tcatcctcgg catcgcggta ctgtccctgc tcggcagcct gcgttcccgc gcatcggacg
    10441 aattggcacg gattttcggc acgcaggcag acatcggcag ccgtgccgcc gacatcggac
    10501 acggcatcaa acaaatcggc aggaacatcg ccgaacaaat cggcggattt tcccgcgaat
    10561 ccgagtccgc aaacatcggc aacgaagccc ttgccgactg cctcgacgaa ataagcagac
    10621 tgcgcgacgg cgtggtacgc ctcaacgaac gcctcgaccg gctcgaacgc gatatttgga
    10681 tagactaacc ttcagacggc atccgacatg aacagccctt ttcacaacat cggcatcgta
    10741 acccgcccca acacgcccga catccaagac accgcgcaca cgctgattac ctttttgaag
    10801 cagcacggct ttaccgtcta tctcgacgaa gtcggcataa aggaaggctg catctatacc
    10861 caagacaccg tcggctgcca tatcgtcaac aagaccgaac tggggcaata ctgcgacctg
    10921 gtcgccgttt taggcggaga cggcaccttt ctctccgtcg cccgcgaaat cgccctgcgc
    10981 gccgttccga ttatcggcat caaccaaggg catttgggct tcctgaccca aattccccgc
    11041 gaatatatga cggacaagct attgcccgtt ttagaaggga aataccttgc cgaagagcgc
    11101 atcctgattg aggccgcact catccgcgaa ggcaaaaccg ccgaacgcgc catcgccctc
    11161 aacgatgccg tcctctcccg tggcggcgcc ggacagatga ttgagtttga agtcttcgtc
    11221 aatcgggaat tcgtctatac ccagcgttcg gacgggctga ttgtctccac ccccaccgga
    11281 tcgaccgcct attcgcttgc cgccggcggc cccatcatgc aggcaggatt gcacgccttc
    11341 acgctcgtcc ccatctgccc gcaatccatg accaaccgcc ccatcgccat tccagacacg
    11401 tccgaaatcg aaatcctcgt tacccaaggc ggcgacgcgc gcgtccattt cgacggtcaa
    11461 acccatatcg acgtgcaaaa cctcgaccgc atcaccatcc gccgctaccg aaacccctta
    11521 cgcatcctac atccgaccga ctaccaatat ttcaaaaccc tgcgccaaaa actgcactgg
    11581 ggcgaacaat tggtctgaac cgacccttac cggaaaacac ctatgaatcc aaaaactccc
    11641 cccgaccata cagaagatgc cgtgcgcctc tccaaacgca tggcgcaact ggggctttgt
    11701 tcgcgccgcg aagccgacgg ctacatcgaa caaggctggg taacggtcaa cggcaaaacc
    11761 gccgtactcg gtcagaaagt ttcaccggcc gaccgtatcg aactaaacaa gaaagcccac
    11821 gaacagcagg cggcacgcat taccatcctg ctgaacaaac ctgtcggcta tgtcagcgca
    11881 caagcggaaa aaggctataa atccgccgcc gaactgatta cccctgaaaa ccgctgggaa
    11941 ggcgacaccg gacgcatccg tttcgatccg aaacacaaaa tcggcctcgc ccccgccggc
    12001 aggctggaca tcgactcggt cggattgctg gtattgacgc aggacggccg tatcgccaag
    12061 cagcttatcg gcgaaaacag cggcagtgaa aaagaatatt tggtgcgcgt gcgcggcaaa
    12121 ttggacgaaa aaggacttgc cttactgaat cacggattga gtttggacgg cgagaaactg
    12181 cgtcccgccc aagtagaatg gcaaaacgaa gaccaactgc gcttcgtgtt gaaacagggt
    12241 aaaaagcggc aaatccgccg tatgtgcgaa ctggtcggac tgcgcgtcgt cgggctgaaa
    12301 cgcatccgca tgggcaaggt caaactcggc aggctgccgc ccggcaaatg gcgttatctc
    12361 gcccccggtg aatcgtttta aataagcatt aaaaaatgcc gtctgaaaat ccttgcgttt
    12421 tcagacggca ttgccttatg cccaaataac ggtttcttcc aagggcctgc gcgctttcgt
    12481 ggcgatttcc tgaacgcggt aaccgaatgt cgcggcgacg gacacgcccc attctgccgc
    12541 atcgaacaaa ccgaactgcc cggacaatac gcgttccata tcggcatagt tgaaaccttc
    12601 caccgggcag gaatcgatac ctgccatcgc cgcacccgtc atcatgttgc ccaacgcgat
    12661 ataggtctgg cggcaacacc agtcaaacaa ggcgcgcgaa tcgtccaaaa tcttgatgtc
    12721 gtcggcttga aacgcctgat acctcgccaa agattttgcc atggcatccg gttcggtaac
    12781 gccgcgccgt ttgaggcttt ccaacataaa cgggctgtcg aagcgggcat ttttctttgc
    12841 caaaaacacc accaaatgac tggcggtatc caaagcatcc gccatacccc aagaaaacgg
    12901 cttgattgcc tgtcggattt cggggttttg aaccacaaca aactgccaag gctccgaacc
    12961 gaccgaactg ggcgacaaac gcccgagttc taaaataaac tgaaaatcct cggcactgat
    13021 tttacgtgcc gcatcgtaat gccggcatga tttgcggttt ttaaatgcgg atagaacctg
    13081 ctctttgctt aatactgcca tcgcacatcc tttcattact tgtattgcaa attgatttca
    13141 aaccgatacg gcactgccgc accatacaat ccgtcctcgc catcaagctt catccggact
    13201 ttacccatct ctctacggct gggaaaacaa agcggcggca gactcgggaa taacgcccca
    13261 atgcctttca gacggcattt gggcatcatt gcaaacgcgc ctacgggtag gcgatatagg
    13321 cataagccga atggttgtgg atagactcga aattctcgct ctcgacaacg aaactcttga
    13381 tgcgtttgtc ggcgatcagc gaagtagcga catcacgcac catatcttcc acgaatttcg
    13441 ggttttcgta ggctttttcg gtaacgtatt tttcatcggg gcgtttgagc aaaccgtaga
    13501 gttggcagct tgcctgcgcc tccacataat caatgacttc ctcgataccg acttctgcat
    13561 cggcagtcag gctgacggta acatgcgaac gctggttgtg cgcgccgtat tgggaaattt
    13621 ctttggaaca cgggcaaagc gaggttacgg gaatcatgac cttcatactg tggccgtatg
    13681 ccccgtcttt gatttcgccc gtgagacaga catcataatc cagtaaggac cgaataccgg
    13741 aaaccggcgc ggttttcttg cggaaaaacg gaaaagaaac gctgattttg ccggcgcggg
    13801 aatctaaaag cgcgaccatt tcggtagtca gcttgcgcaa ttgtgcaaaa tccaaggctt
    13861 cggcatgttg ctccatcaat gcgacaaaac gcgacatatg cgtccccttc tgctcagcag
    13921 gcaggtaaac cgtcatggtc agacgggcaa tggtggactg gatgccttct gcagtttgca
    13981 gggtaatcgg aaagcgcagg tctttgatac cgacctgatt gatcggcaaa ttgcgcaaat
    14041 ctcggctgga ttgcacgtct gcaatagtgt tcatgagttg tttttcctta atattcatgt
    14101 aattgttgag atgtgtgcat ggtatggcgt ttccgccggg gaatataccg tcaatctgca
    14161 aatgcgagat tatatcactc gcttttagca gctgcattga ttgtttctat ttttcagata
    14221 gtgataaaat cttcgattat tcacatctac ataggcagag accaaatgaa attcgccacg
    14281 aaagccattc attccagcta cgattgcgac gaacacaacc gcgcgctgat gccgccgatt
    14341 tatcaaaaca gtatgtttgc gttgcacgag attggcgaaa atgtgcctta ccgttattcg
    14401 cgcctgagca acccgacccg tcagatttta gaagacaccg ttgccgattt ggaacacggt
    14461 gcggcaggtt ttgcgttttc cagcggtatg gcgggaattg atgccgtatg gcgcactttc
    14521 ctgcgcccgg gcgataccat tgtcgccgtc gccgatattt acggcggcgc ttatgattta
    14581 ttggtcgatg tttatcaaaa atggggggtg aacgttgttt ttgccgattt aggcaatccg
    14641 gataatttgg acgaactgct taaagcgcac aaggtcaaac tggtttggct ggaaacgccg
    14701 tccaatccac ttttacgctt ggtagacatc aaagtccttg ccgcaaaagc caaagcagcc
    14761 ggtgcgctgg tcggtatcga caacactttt gccacgccgt atctgcaaca gccgttggat
    14821 atgggttgcg attttgtatt ccattccgct accaaatatt tgtgcggcca ttccgacgtg
    14881 ttgatgggca tcgtcgttgc caaaaccaaa gaactggcgc agcctttgca cgatatgatg
    14941 gtgcataccg gcgcggttgc cggcccgctg gactgctggc tggtgttgcg cggcatcaaa
    15001 acactggctc tgcgcatgaa cgcccattgc caaaacgcac tcgaaatcgc gcgccgtttg
    15061 gaagcccatc ctgccattga aaaagtgttc catcccggcc tgccgtctca cgaacattac
    15121 gaactggcga aaacacaaat gcccaaaggc atcggcggcg tggttacggt ttatctcaaa
    15181 aacgacacgc gtgaagcggc aaacagcgtg attaaaaaca tgaaactggt caaaatggct
    15241 tccagcctcg gcggtgtgga aagtttggtc aaccattgct attcccagtc ccacagcggc
    15301 gtaccgcatg atgtgaaaat ggaaatgggc atcaaagtcg gtctgctgcg tttctccatc
    15361 ggcatcgaag acgcggacga tatttggaac gatatttccg ccgcactcga tacgactttg
    15421 taaactgtaa aaatgccgtc tgaaaccatg gtttcagacg gcatttcaat taacccggcc
    15481 gaaaatcaac gcttcaacat ctttgccgcc tcaatagcgt aataggtcaa aatcccgtcc
    15541 gcacccgcac gtttgaatgc cagcaggctt tccaaaacca ctttgccgcc gtccagccag
    15601 ccgttggcaa tcgctgcctg caacatcgcg tattctcccg aaacctgata ggcataagtc
    15661 ggtacgccga actcgtcctt cacgcggcgg acaacgtcca aatacggtaa accgggcttc
    15721 accatcacca tatccgcacc ttcccgaata tcgagcgcca cttcattcag cgcctcatcg
    15781 gtatttgccg gatccatctg gtaggttttc ttgtccgcct tgcccaaatt gcccgaactg
    15841 cccaccgcat cgcggaacgg cccgtaaaac gccgaagcat atttggcgga atacgccata
    15901 atccgcgtgt ggatatgccc ggcatcctcc aacgcctcgc gaatcgcacc gatacgcccg
    15961 tccatcatat cggaaggggc aaccacctgc gcgcccgctt cagcgtggca caaagcctgc
    16021 ttgaccaaaa cctctacggt ttcatcgttc atcacataac cgttttcgtc cgtcagcccg
    16081 tcctgaccgt gaaccgtata aggatcgagc gcgacatccg tcataatgcc cagttcggga
    16141 aacctctcgc gcaaggcgcg gacagttgac ggcacgagtc cttcgggatt gtacgcctcc
    16201 tgcgcacgct cggttttgtt tgccgtaacc acggggaaca gtgccaacat cggaataccg
    16261 agctttaccg cctcttccgc cgtaaacagc agcctgtcca aactttgacg cttcacaccc
    16321 ggcatagaag gcacatcctc ctcgcgcgcc gacccctcca atacgaacac cggataaatc
    16381 aaatcatcgg cggtcagcgt gtgttcgcgc atcaggcggc gtgaaaaatc gtccctgcgc
    16441 atacggcgca tacgcgaagc cggaacattg cggtaaggaa actgcataag ccctccaatc
    16501 atcctgtcaa caattcaaac catacggaag ccgccgcaac ttgcagacag catcgggcgg
    16561 caatcaaaat ccgttttcac cggaaagggt ttcggcccgc cgaaatgccg cgccgaagga
    16621 cggcgtcccg cccattaaag aacaatagca aaacaacagc ttcaggctgt cagccttcga
    16681 tattcttacg ccacaatacc aaaagtttcc cgatatgctg aacaggttgc gcgtcaaccg
    16741 cctcacataa ggcagtgcag atttcgatac gttcggcacg gtcgtcgccg aatacgcgca
    16801 ctttaatcag ctcatgcgcc gtcaatgccg catcggtttc cttgatgacc gcgtccgtca
    16861 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcagttcca
    16921 aaatttcttt ggtgttcaat ttggtatccg tcattttcct actgcttgaa taaaaagaat
    16981 aatgcggcat tgtacgcgat ttggcacgac atcggcaaaa ttaacgtaca atacgcggtt
    17041 tccattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    17101 acgaacacgt caacgaccac tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    17161 ccgcatacaa acttttggaa atcaacgaaa aagacaaatt aatcaaaccc ggcacggtac
    17221 ttgccgactt gggcagcgca ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    17281 cttccggagc agttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtctcct
    17341 tcattcaggg cgacttccgc gagaacgacg tactggcaca attcgaaacc ttgttggaca
    17401 accgcccgct cgaccttgta atttgcgata tggcgcccaa tatgtcggga aacgccgtaa
    17461 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt agacttcgcc tcgcaacacc
    17521 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    17581 tggcagccat gcgcgaaatt ttcggcacgg tgcagacgcg caaacccgaa gcctcgcgca
    17641 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    17701 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    17761 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    17821 tagacggtaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc caacaggtca
    17881 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    17941 agggcgagcg caccgacaaa agcaccttct tcaccaacgc gcctttggac gacaacctga
    18001 ttaaaacact gctcgacaaa aacgtccgtg taaaagtaac gccggaagaa aaaccgagcg
    18061 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    18121 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    18181 gccgcgcccg cctactggac aaagatgcca acaaagttac ctttgccgat gtcgccggct
    18241 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagcg ccgaaccgct
    18301 atcaaagcct gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    18361 gtaagacgct tttggcgaaa gcgattgcag gcgaagccgg cgtgccgttc ttcagcattt
    18421 caggttccga ctttgtcgaa atgttcgtcg gtgtcggtgc aagccgcgtc cgcgatatgt
    18481 tcgagcaggc gaagaaaaac gccccctgca tcatctttat cgacgagatt gacgcagtcg
    18541 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    18601 aattgttggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    18661 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgtttc gaccgccaag
    18721 tggttgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cactctaaaa
    18781 aagtgccttt ggacaaatct gtggatttat tgtccctcgc gcgcggcacg ccgggttttt
    18841 ccggcgcgga tttggcgaac ttggtcaacg aagccgccct gtttgccggc cgccgcaata
    18901 aagtcaaagt cgatcagagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18961 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat cacgaatccg
    19021 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    19081 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    19141 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    19201 acatcttcgt cggacgcatc tccaccggcg catcaaacga ctttgaacgc gcaacccaaa
    19261 tggcgcgcga aatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    19321 cggaaaacga aggcgaagtc ttcttgggac gcagcgtaac ccgttcgcaa aacatttccg
    19381 agaaaaccca gcaggacatc gatgcggaaa tccgccggat tttggacgag cagtatcagg
    19441 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    19501 tggaatggga aaccatcgac cgcgatcagg tactggaaat catggcgggc aaacaaccca
    19561 gcccgcccaa agattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    19621 cgccgcacgc tccgactcgg gaagaaaccg aagcacctgc cccggcagac accgcttcga
    19681 cagagtccga gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    19741 agctgccgtt ttcccatcct gttttcagac ggcattttcc gctcaatgcc gtctgaaacg
    19801 ccaaagccgt atcggttttt gcgaatctgc gatatggcgg tttaaattga aatcagaacg
    19861 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    19921 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19981 caaaatgttc gagctggtcg ataaggttga ggattacccg aactttctgc cgtggtacag
    20041 caaaaccgag gtcatcggac gcaacggcaa cgaactgaag gcgcggctgt ttatggacta
    20101 tatgcacgtt cgccaatcat tcgccacgca caaccgcaac attccgggca gggaaatccg
    20161 tatggaactg ctcgaaggtc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    20221 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    20281 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    20341 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacgggctg cccgaccgac
    20401 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa
    20461 gcggtttgga cggcatattt gaagatttaa acctgcattc cgcgcctttg ggcattttcg
    20521 gcaaagccgt caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    20581 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    20641 catgccgtct gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac
    20701 aaaatcaaaa tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac
    20761 aatgcgggtt tttggttcct cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa
    20821 gaaaaaaaat tcttcggcga agtcgcccgt gccgccctgc ccgacggcga cgtttggctg
    20881 ctcaaacctg ccacgttcat gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc
    20941 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga
    21001 cggattaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    21061 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac
    21121 cgcaacctcg tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt
    21181 gacgatgtcg tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa
    21241 gaagcaaccc gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg
    21301 catgttcccg atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    21361 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    21421 gacgcaccat ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    21481 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    21541 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    21601 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac
    21661 accttgccct gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac
    21721 ttcccatccc ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat
    21781 gcgcgcaggc atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata
    21841 tggcaggtac ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg
    21901 gtttatttcc cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat
    21961 atcaatccca tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca
    22021 gattacaaat atactcgaac cagttgtaga atcggcggtt ccacaaaccg tttcggattg
    22081 cacgacacaa aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc
    22141 aaaggacaaa acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac
    22201 cgactttaag ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca
    22261 catcctgcct tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc
    22321 actttggttt accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc
    22381 cgtcgtactc ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc
    22441 gattatctac atttttttcg gaggcttcgc gcttgccacc gccctgcata tgcagcggct
    22501 ggaccgtaaa atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt
    22561 tttgatgttg ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc
    22621 cgcgatgatg ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca
    22681 caaaacctac gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg
    22741 cacgctcgtc ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt
    22801 cggctggatg aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc
    22861 cctgtacgtc atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat
    22921 cccttggacg ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg
    22981 gatattcagc tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc
    23041 cctgagtgcc gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg
    23101 caataccgac tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt
    23161 gaaaacatcc ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc
    23221 gcccgcattt ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac
    23281 cagcaacacc gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat
    23341 ggggctgccc gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat
    23401 gctgccggtt gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg
    23461 cgaaatgatg aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg
    23521 ggcttatgct gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat
    23581 ttttcagacg accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg
    23641 attctaacga aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg
    23701 aactcgattt cgacaaagcc cctgttaccg ccaaaaactt cgagcaatac gtcaaagacg
    23761 gcttctacga cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg
    23821 gcatggatga aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca
    23881 acggcctgcc caacgataaa tacaccatcg ccatggcacg cacttccgac ccccattccg
    23941 ccagcgcgca attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag
    24001 agctgtacgg caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg
    24061 acggttttga tgttgtcgat gccatcgaag gcgtatccac caaacgccac ggctaccatg
    24121 acgacgtacc gagcgaacct gtcgtcatca ttaaagccga agcggtataa accggcaatc
    24181 cgaaagcagc ctgcacaaag gctgcttttt ttgtttcagg cggcataaaa tccaagcaca
    24241 tatgccgtct gaacacctcc cgcaagcctt cggacaatgt tgcaacgtgt taaaatagcc
    24301 tcctttcctg aaaccacaac aaggaaacca tcatggcaaa tattgcccgc gacatcttca
    24361 aagcctacga catccgaggc attgtcggca aaaccctgac cgacgatgcc gcctatctca
    24421 tcggcagggc catcgccgcc aaagccgccg aaaaaggcat gacccgcatc gcgctcggac
    24481 gcgacggacg cttgagcggt cccgaactga tggagcacat ccaacgcggc tttaccgaca
    24541 gcggcatcga cgtcctcaac gtcggtatgg tcgccacgcc catgctctat tttgccgcca
    24601 tcaacgaatg cggcggcagc ggcgtgatga ttaccggcag ccacaatccg cccgattaca
    24661 atggtttcaa aatgatgctc ggcggcgaca cgctcgcagg cgaagccatt caagaacttt
    24721 tagccattat tgaaaaagac ggttttgttg ccgccgacaa acaaggcaac gtaaccgaaa
    24781 aagacatctc cggcgaatac cacaaccaca tcgtcggcca catcaaactc aaacgcccga
    24841 tgaacatcgc tatcgacgcg ggcaacggcg tgggcggcgc gtttgccggc aaactctata
    24901 aaggcttggg caacgaagta accgagcttt tctgcgacgt ggacggcaat ttccccaacc
    24961 accatcccga cccatccaaa cccaaaaacc tgcaagattt gattgccgcg ctgaaaaacg
    25021 gcgatgccga aatcggcttg gcgtttgacg gcgatgccga ccgcttgggc gtagttacca
    25081 aagacggcaa catcatctat ccagaccgcc aactgatgct tttcgcgcaa gacgtgttga
    25141 gccgcaatcc taaagccaaa gtaattttcg acgtcaaatc cacccgtctg cttgcgcctt
    25201 ggattaaaga acacggcggc gaacctgtga tggaaaaaac cggccacagc tttatcaaat
    25261 cctccatgaa aaaaaccggc gcgccggttg ccggcgaaat gagcggacac atcttcttca
    25321 aagaacgctg gttcggcttc gacgacggtc tgtacgccgg cgcacgcctc ttggaaatcc
    25381 tgtctgcctc cgataatccg tccgaagtgt taaacaacct gccgcaaagc atttccacgc
    25441 ccgaactcaa catcgccctg cccgaaggca gcaacggcca tcaggttatc gacgaactcg
    25501 ccgccaaagc cgaatttgaa ggcgcaaccg aaatcatcac catcgacggt ctgcgcgttg
    25561 aatttcccga cggcttcggt ctgatgcgtg cttccaatac cacgccgatt ctggtgttgc
    25621 gttttgaggc ggatacgcaa gaagccatcg agcggattca aaaccaattc aaagccgtca
    25681 tcgaaagcaa tccgaatcta atctggcctc tgtaaacaca ggaaaaatgc ttaatcaaat
    25741 cttggtagat tggatttggc ttaaaaatct ccgcgtccgt tcgtgtttgg ggtggtcgaa
    25801 caactctttc gggctgccct gctctacgat aacgccgccg tccatcacga caacggtcgt
    25861 ggcaacttcc agcgcgaact tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg
    25921 cgccaattcc ttcatggcgt tcaacacgtc ttgcaccaat tcagggtcga gtgcggaagt
    25981 gggttcgtca aacaacatca gctcgggctg aatcgccaat gcgcgggcaa tgccgacgcg
    26041 ctgctgctga ccgccggaaa gctggtaggg atacaaatcc actttgtcgc ccaagccgac
    26101 tttttccagc agtttcagag cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc
    26161 aaccggtcct tccattacgt tttccaaggc ggttttgtgc ggaaagaggt tgtattgttg
    26221 aaacaccatg cctgatttgc ggcgcagtgc caaaatatcg tgtttgcttg gttttttaga
    26281 aaaatcgatt ttcagcggtc gctcgttgtc gaactcgatt tgtccgtctt cgggcatttc
    26341 caacgcgttt aggcatcgca gaaacgtcgt tttgcctgag ccggaaggcc cgaggataac
    26401 gaccacctgc cctttgccca catccaaatc gatgccgcgc aaaatagtgt tttcgccgaa
    26461 ggtcttatgg atattgcgga ttttaatcat aacaactcct tatttggcga cataacggtc
    26521 gaaacgtttt tccaaacgtg cctgaatcag gaacagaacc ttgcagaaac accagtaaac
    26581 caatgcggct tcgatataga cgggcaaaaa gtcgtaagtg cggtttgccg tttcctgcgc
    26641 gacgcggaac aattcagtta ccgttaccac cgctgcgagc gaggtgtttt tgaacaagcc
    26701 gataaactcg ttgctcaaag gcggcacggc aacgcggaat gcctgcggcg cgacgatgcg
    26761 gcggaacgtc tgcatatagg tcataccgat ggagaaacct gcttcccatt ggcctttagg
    26821 tacggacaaa attgccgcgc gtatggtttc ggaagcgtat gcaccgacat tgagcgagaa
    26881 gccgataatg gcggcgggaa tcggatcgat atagataccg acggacggca gcccgtaaaa
    26941 cacaatcaca agctgcacca acagcggcgt accgcgaatg acggaaatat aaaattccac
    27001 caatttcagc aggattttcc gcacgatgct gccggcgggc ataatccgca ccaaagccac
    27061 ggctaccgca atcatcatac cgataacgaa agaagctgcc gccaaaggca gagagaccgc
    27121 gaagccggct ttgaccatag gcaaaaacgc gctgacaatc atatcggcgc gtgtttccgt
    27181 cataaacggc agcgaagcaa ggaaattatt gaacactgat gtcttttccg aagaattgtt
    27241 cgcccagttt tttcagcgta ccgtcggctt tcagctcgtt gattgccgta ctgaatttcg
    27301 ccacggcttc gtcattgccc ttgttgacaa tcaggccgga accgactttt tcatcggcag
    27361 gtgcggacca aacgattttc acgcccgcat tcgggttttt cttcagatag tccaaaaccg
    27421 ccaattcgtc gttcagggtt gcatcggcac gtttttgttc aatcagggtc agcgattgcg
    27481 ccaaaccgtc aacagccacc aaatctgcgc ctgcagcttt ggctttttcg ccgtagttgc
    27541 tggtcaggga ttgtgcggtt ttcacgcctt tgatgtcgtc gatagatttg atgttgctgt
    27601 cgttacgggc aaccaatacg gcaccgctcc agctgtaagg atcggatttg tcgaatgtcg
    27661 cttggcgttc ggggctggtc agaccgactt ggtttgccac cacgtcgaaa cgccccgcct
    27721 tcaaacccgc catcatcgaa tcccattgcg tttctttaaa ctcgactttc acgcccagtt
    27781 tttccgccac ggcgcgggtt acttccacat cgtaaccggt cagtttgccg tccttgtcgt
    27841 ggtaggtaaa cggtgcgtaa gtgccttccg tgccgacggt aaccgtgcct ttattgttga
    27901 tgcgctcgat taaagaaccg gaaactgccg attgtgcagg cgcggaagat gctccgctgc
    27961 cgccttccga accgccgcag gccgccaaaa ccaatgcggc aataccgccg agtacgaatt
    28021 ttttcaacat cattctctcc tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc
    28081 ggcaaacaca aagaatactt tttttatatg gttattccaa atcggaatgg aaaagatatg
    28141 ccgaagccgc cggaagcgga aataaagggg gcgggaaagg cggcgggcgg cacgaaaccg
    28201 aaaagcggca tcaggtatgc aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc
    28261 ctgccgattc ggtgctataa tccatccgaa acagatgaca ccaaaaagga acatctcatg
    28321 aacacgcccc aatccgccat cattcccgac cacgcccaag ccggcatctt tatcgaagcc
    28381 gacttcgccg ccaaccgcct caacgatatt aaatccgcct gccgcgcttc gctcgacgcg
    28441 ttatccgcct tgaaaacccg ctttccggac gatattttgg gtctgaccat cgccttcggc
    28501 agcaaagcct gggcaacatt cggacatacg gacgaaggca gtgaaatcaa acccttctcc
    28561 gaaatgggca acgggcttgc gccgtctacg cagcacgata tgtccatcca catccaatcc
    28621 ttccgccaaa acgccgccta cgcgcttgcc caatccgttt tgggcgcgtt cggcgacagc
    28681 atatgcgtcg cgtccgaaga acacggtttg cgcctgtatc aggatcgcgg gctggacggt
    28741 ttcgtcgacg gcacggaaaa cccgcagggc gatgaaaatg tccgcgaagt cgccatcatc
    28801 cccgaaggaa aacccgatgc gggcggcagc tatgtcctgc tgcaaaaata cctgcacgat
    28861 ttgaaaaaat gggatgccgt ccccgtcgcc gaacaggagg cctcggtcgg acgcggcaag
    28921 gaaaccgacg acgaattcgg ccacgatgtc cgcctgcccg attcgcacct cggccgcgtc
    28981 aacctgaaag aaaacggagt cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa
    29041 atcagcggcg aacacggttt gatgtttacc gcatactgcc gcacgctgca caatatcgaa
    29101 gcgcaattat tgagtatgtt cggcgacacg gatggcaaaa ccgacctgct gctccggcac
    29161 ctctccgccg ccgtttcggg cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac
    29221 ctctaagcca gacgatgccg tctgaaaccc tttttcagac ggcattttcc cgttttaccg
    29281 tccaaccgtt ttccgagcgc gcactatgtc tgcacccatc caagcatttg acccgctgac
    29341 cgtccccatt tccggcacca acctgattga ggcctctgcc ggcaccggca aaacctacgg
    29401 catcgccgcc ctgtttacgc gcctgatcgt attggaacaa aaaaacgtcg agcgcgtatt
    29461 ggtcgttacc ttcaccaaag ccgccaccgc cgagttgaaa acacgcctgc gcgcacgttt
    29521 ggacgatgtg ttgcaggttt tagaaagtaa agaaattgcc aaacttggag acgacacgct
    29581 ttcagacggc atcgccgcct actgcgccga acaccacaaa ggcgacacct tcctgcccgc
    29641 actcttaaaa caggctttgc aaaaagaggg ccggacgcgt ctgattgtcc gcctcaaggc
    29701 cgccatcggg caattcgaca acgccgccat ctataccatc cacggcttct gccaacgtat
    29761 cctgcgcgac tacgccttct tgtgccaagc accgttcgat gtcgaactga ccgaagaaga
    29821 cggcgaccgc ctgcttatcc cggcgcaaga tttttggcgg gaacgcgtca gcaatgatcc
    29881 ggtgcttgcc gcattggcgt ttaaacgcaa agctgtgccg caaaccgtcc ttgcccaaat
    29941 ttcgcgctat ctttcccgcc cgtacctgaa tttccgccgt ccgcaggcgg atttgaaaca
    30001 ggcgcagcgc aacgccgaaa cctcatggca aaccatctgc cgcctgctgc ccgagctgga
    30061 agccggcttt tggcgcatcc atcccgacct caacggcaac agttaccgca aaaacagctt
    30121 cggcaacctt tttaaagaac tggcacaaaa atccgccgcc ggacaactgc cctttctgga
    30181 caaagacacg cacgacagac tcttgaaact tgcgtccgac aaactcgaag ccgcccttaa
    30241 aaaaggcaaa acgcccgatg cggcagcatt tgccgaattg cagaaactgg cagacttcgg
    30301 gcgcgatttg aacgcactcg aagaagcgga agaagcaaca atgatccggc tgcaactgga
    30361 tttaatcgga tacctcaacc gcagccttgc cgagatgaaa aaatcgcgcc gcgaacgcgg
    30421 tttcgacgac ctgctgctcg atgtccacac cgcgctgacc gacaatccgc acgccgaaac
    30481 cctcgcccgc gccgttgccg aaaactggaa aaccgcgctg atcgacgagt tccaagacac
    30541 cgacccgctg caatatgaaa tcttccaaaa gattttcatt gcccaaaacc gcccactgtt
    30601 tctcgtcggc gatcccaaac aggcgattta cagctttcgc ggagcggaca tttacgccta
    30661 cctgcaagcc gccggagacg cgcggcaccg ctacacgctc gccaccaact accgcagcca
    30721 cgccgcgctt atcggcagca taggcgcgct gttccgcctc aaagaacgcc cgttcgtttt
    30781 ggaaaacatc ggctattcgg aagtcggtgc ggcgcgtgcc gaaagcaggt tgtcccccga
    30841 acgtcctgcc gtacaggttc gttggctgca cgaaaacgac aatgaaaaag ccaacaaaga
    30901 cgttttgcgc cgccgtgccg ccgactattg cgccgacgaa atcgcccacg cgctcaacga
    30961 agccgccgga ggccgtctga atttcaaagg ctgcccgttg cagtcgggcg atattgccgt
    31021 gctggtacgc acgcacaacg aggcggtgat ggtttccgcc gccctgaaaa aacggcaggt
    31081 gcaaagcgtc ctgctttcgc gcgaatctgt gttcgcctcg cccgaagccg ccgccctgtc
    31141 cgcactcatc ggcttctggc tcgaaccgcg ccgcgccgga acgctgcgct ttgtcctgac
    31201 aagcggcata ttcggttatg acgcgcagca attgcacgac ttcaaccaaa acgaaagcga
    31261 gattttgcat tgggcggaat ctgcccgaac cgcactcgac aactggaatc aatacggcat
    31321 tttcgccgct atgcagcaat tttcccaaac acacggcatc gaaacgcgcc tcttaagccg
    31381 gaacaacggg cgcagcctga ccaactattt ccaactgctc gaactgcttg ccgccgaaga
    31441 cgcgcaaaac cgcaacccag ccgcgctgca caaatggttg cgcgaccaaa tcagcctggc
    31501 cggcaacaac agcggcgaca accgcgccat ccgtctggaa agcgacgaag atttggtcaa
    31561 aatcgttacc atgcacgcct cgaaaggttt gcagtatccg ctggtgtact gcccgtttgc
    31621 gtgggacgcg caagataccg ggccgtccga ctggcaaatc ctccaccaaa gcgcaaaccg
    31681 aaccgaactg ttggcaaagg cgcaactgtc ggaagacgaa cagaaacaat acgccgatga
    31741 agaaatggcg gaacgcctgc gcctgcttta tgtcgcactg acgcgtgccg aggaacagct
    31801 caacatctac gccgcgtact ccaccaatac tgccgacaac cccctcgcct acctgattga
    31861 aggctcgccc caagacagcc gtgaaaccgt ccgccgtgcc tatgcgtgtg aaaaagacgg
    31921 catcgcgatg ctcaaacgca actggcggcg cgtggcggac aacgcccctt ccggcacaaa
    31981 tttcgccttc accgaaaatg cgccgccccc tgccgcctat cgcagcaacg ccggtcaaac
    32041 cgccgaattt gccgcaaaca gcattcccga acgcggattc cgatttgtcc gccacactag
    32101 ctttaccgcc ttaagccgcc atacccaaac gcccgacggc ggcgaagaag atgcctgccc
    32161 gtccttggat gccgccgaaa cctcggtgcc ggcgatgccg tctgaaacgc cgacggcttc
    32221 agacggcata tcgatacacg attttccgaa aggcacgcag gcggggctgt gcctgcacga
    32281 aattcttgaa gatttcaaat tcggacaagc agccgccgga caggaaaccc tcattgccga
    32341 caagctgaaa aaatacggtt ttgaagaaat atggctgccc gccgttgccg aaatggcgga
    32401 agcctgccgc aaaacgccgc tgacgggggc atacgacctg tccgacatct cacccgagtg
    32461 ccgctgtccc gaaatgggct ttaccctcca caccgaagac ttcagcctca aacgcctgcg
    32521 cgactggttt gcccgcgacg acatcaggct gcccgaagtc tgccgtgccg ctgccgaaac
    32581 gctcgacttc cacaccgtca acggcttttt aaacggcttt gtcgatatgg tctgccaaga
    32641 ccccgacggc aatatctgcg tcatcgacta caaatcaaac cacctcggta cggacgcatc
    32701 cgcctacacg caacaggcaa tggacgaagc cgtcgcacac caccactatt accttcaggc
    32761 actgatttac gccgttgccg ccgcacgcta cttcaagctg cgcggacaac cgcccgccgc
    32821 cgtttccgtc cgctacctgt ttttgcgcgg attggatggc aaaggcggcg gcgtttggcg
    32881 ttgggacata gatgccgccg ctttggaaca gataaaataa cttcccgtcc tttcggacaa
    32941 caaccgcagg aaaaccctat gaatatcaaa cacctgatta ccgccgcact cattgcctca
    33001 gccgcctttg ccgcgcaggc agccccgcaa aaacccgtat ccgccgccca aaccgcgcaa
    33061 catccggccg tttggatcga tgtccgttcc gaacaggaat ttagcgaagg gcatttgcac
    33121 aacgcggtca acatccccgt cgaccaaatc gtccgccgca tacacgaagc cgcgcccgac
    33181 aaagacacgc cggtcaacct ctactgccgc agcggacggc gtgccgaagc cgcccttcaa
    33241 gagctgaaaa aagcaggtta tacaaatgtt gccaatcacg gcggttatga agacctgctc
    33301 aaaaaaggga tgaaatgacg caatgccgtc tgaaaggaca ggctttcaga cggcatacgg
    33361 cgttccggca cgcggcgcaa catcgggcgc ggcgcgcgcc gccccgtccg aagcctgata
    33421 aacaaaccga taaggaacaa tgatgtttca cgacacataa agcaccgccc tatgtgttgc
    33481 cctgatttgg aaagggttac acccctcccg aataaagtct gattctaccg tttcaaccaa
    33541 aaaggaaaaa ggaaatacga tgaaacacat actccccctg attgccgcat ccgcactctg
    33601 catttcaacc gcttcggcac accctgccgg caaaccgccc acccaaaacg aaaccgctat
    33661 gaccacgcat accctcacct cgaaatacag ctttgacgaa accgtcagcc gccttgaaac
    33721 cgccataaaa agcaaaggga tgaacatttt tgccgtcatc gaccatcagg aagccgcccg
    33781 ccgaaacggc ttaacgatgc agccggccaa agtcatcgtc ttcggcacgc ccaaagccgg
    33841 cacgccgctg atggtcaaag accccgcctt cgccctgcaa ctgcccctgc gcgtcctcgt
    33901 taccgaaaca gacggcaaag tacgcgccgc ctataccgat acgcgcgccc tcatcgccgg
    33961 cagccgcatc ggttttgacg aagtggcaaa cactttggca aacgccgaaa aactgataca
    34021 aaaaaccgta ggcgaataag cccgtcgccg acatatggcg gatggaaatc aaatgccgtc
    34081 tgaaacccga tttcagacgg catttccttt caatccctat cttcccgaca aatatcaatg
    34141 gcttcctgca aactcgaaac gccgtagatt ttcaggttcg gaaactcttt ggcgttgcgc
    34201 ggcatattgg ctttggggac gatggcgcgt ttgaagccga gtttttccgc ttctttgagc
    34261 cgctcttgtc cgcgcgcgac ggggcggact tcaccgctca aaccgatttc tccaaatgca
    34321 accatttttt cgggcagcgg gcggttgcgg aagctggaaa gcatcgcgag gatgactgcc
    34381 aaatccgccg ccggttcgcc gattttcacg ccaccgacgg cgttgaggaa cacatcctga
    34441 tcgaaacagg cgatgccgcc gtgtcggttt aacacggcga gcagcatcgc gaggcggttt
    34501 tgttccagtc cgacggtaag gcgtttgggg gtgaatccgt gcgcgtcatc gaccaatgcc
    34561 tgaatttcaa ccaaaagcgg tcggctgcct tcctgcgtaa ccaaaacgca cgaaccgggc
    34621 gtgtcgtcgc ggtagctggc aaggaagatg gcggacgggt tggacacacc tttcaaaccg
    34681 ttttccgtca tcgcgaacac gcccagttcg tttgccgcgc cgaagcggtt tttgatggcg
    34741 cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat acagcacggt atcaaccatg
    34801 tgttccagca cgcgcgggcc ggcaatcgcg ccgtctttgg tcacgtgtcc gaccagtatc
    34861 atggcgatgc ccatctgttt cgccatgcgc gtcagttggg cggcacactc gcgcacctgc
    34921 gacacagaac cgggggcgga agtgatttgg tcggaataca tggtttggat ggaatcgata
    34981 accacgactt cgggctgatg ctgtttcaac gctgcttgaa tcgcttccat gcggatttcg
    35041 gcaagcaggt tcacgccttc ggtgggcagt tccaaacgct gcgcgcgcag ggcgacctgt
    35101 tgggcggatt cttcgccgga aacgtatagc actttacggc tttgcgccat tttggcgatg
    35161 gtttgcagca gcagcgtaga tttgccgatg ccggggtcgc cgccgagcag gatgaccgca
    35221 ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc ccatgccggt cggattgcgc
    35281 ggcacttcgg tggcggtcac ggaggagagg gattggacgg tcgaggtatc cgccgcccaa
    35341 gattggaagc gggcgttttt cggctcgggc gcggccaagc tttcctgaag tgtgttccac
    35401 tcgccgcaat gcgggcattt gccctgccat ttcggggaag tgccgccgca ttcggtgcat
    35461 tggtaaaggg ttttaagcgt ttttgccatc ggttttccct acatctgaaa acaaaaatgc
    35521 cgtctgaaaa tagggacggt tgggattgcg ccaatacggc aacaccgccg ccgtcatgta
    35581 ctatccgtac acggcggata ttgccgcatt gccttatttt taccctaacc ggccatctct
    35641 cggcttcaaa cggcatatcg ggcagtttcc tgccggctta cccgccgtga tactgccgcg
    35701 acagctcgtg tacggtgtcg actaagattt tggcgtgttc ggggtcggcg tgttggttga
    35761 tgccgtgtcc gaggttgaag acatggccgc tgccttgtcc gtagtcggct aggatgcgtg
    35821 cgacctcggt gcggatggat tccggcgtac cgaagagggc gaacgggtcg aagttgcctt
    35881 gcagggcgac ttgcttgccg acgcggcggc gtgcttcgcc tatgttgcac gtccagtcca
    35941 agcccaatgc gtctgcgcca atttctgcca tgctttccag ccacagcccg ccgcctttgg
    36001 caaatacgat aacaggcacg cgtctgcctt cgctttcgcg tttgagtccg gcgacgatct
    36061 ggcggatgta tttgaggctg aattctttaa acgcctcatc gctcaagacg ccgccccaag
    36121 tgtcgaaaat ctgcacagcc tgcgcgcccg cgtcgatttg ggcgttgaga taggcggtaa
    36181 cggcttgagc attggtatcg aggattttgt gcagcaaatc ggggcgcgag tacatcatgg
    36241 ttttgatggt gcggaattct ttgctgctgc caccttcaac catgtagcag gcaagtgtga
    36301 acgggctgcc ggagaagccg ataagcggta cgcggccgtc cagtgcttta cggatggagc
    36361 tgacggcatc aaagacgtat tgcagttttt ccatatcggg gacgtgcaat ttggcaatgt
    36421 cgtcctcgtg ttgcagggcg cgtttgaatt tcgggccttc gccttcggca aaatacagtc
    36481 ccaagcccat tgcgtcaggg acggtcagga tgtcggaaaa caaaatcgcc gcgtccaaat
    36541 cgaaacgttc caaaggttgg atggtaactt cggtcgccaa ttcggtgttt ttgcacaaat
    36601 cgaggaagct gcccgctttc gcgcgtgtgg ctttgtattc gggcagataa cgccccgcct
    36661 gacgcatcat ccaaatcggc gtgtattcga cgggctgttt aagcagggcg cggaggaagg
    36721 tatcgttttt taaaagagtc atacgggttt tctgtcagtc tgtttgtcaa gtgaaatggt
    36781 tttcagacgg catttcaacg atgccgtctg aaggtttcaa caggtttcgg cggaaggtcg
    36841 gttttcctcg gcaacgcttg ccaaaaccaa gttgcgctgc gcctccgcct tctgcggttc
    36901 tccggtttcg tcaaaaacct ttgccaaaac caaacgcgcg gaaatactcg gctttaatgc
    36961 aatgctcgct tcaaggtagc cttttgcctt gccccaaagt ttgcggccgt aggcgagccg
    37021 accgagatac atcagcagaa gcgcgttatc gggctgttct ttcagccaag catcggcaaa
    37081 atcgatggct ttctgctgat cgcgttcgcc caaaaagcgc acgctttcga caaaggcttc
    37141 caaaagttcg ggtcggcggc tttgcggata atgctgtttg acccatttga ccgcatcggc
    37201 atacagcccc aaacgttcgt acttttccgc aaccgatacg ctcaattccc cgtttttgag
    37261 gctgtcggga atccgcttca ggcaggtttt caaagcggcg gcatcggcag catccgccag
    37321 ctggcggcgg tatgcccaat tttgataccg ttccatttcc gatttgccca acgcgcccgc
    37381 cttggaaagt ttttcggttt ttgccagaac ctgcaacgcg tcgcccctgt cgaaagcgta
    37441 acgaagttgc agacgcacga ggcgcgtaag gttggcattc atcttcgccg ccgcatgaag
    37501 attggcttcc gccgcttcgt aatcgcgccg gttcaacgcc gattccgcca gcagaagata
    37561 acgggaaagc tgctgttttt ccggcagttt ggcgatttcg gcaaggtagc ggtcgcgcag
    37621 ctcgatgttt tccatctgcc cggcggcgtg cgcgcccagc atcaatgcca aagtacggtt
    37681 gtctccggct tctttgttgc ccaacacgcg cgaggcttca agttccgcct tttcaaaacg
    37741 cccttcaaaa tacgccaaac ccgccttgtt caaggcaagc gcggccttgc ggcctttacg
    37801 cgccgaaccg aaacgctgca tcttttcggg gatattgaat acgccgataa tgaatttaaa
    37861 caggaaatac cacaccacga cggcaatcag cgaacctaac acaaaagcgt gcaggttgat
    37921 tctgagcatg gtctgtccga gtacgatata cacgtcgccg gtgtaaatgc ccgaagccag
    37981 cgccagtccg acggcggcgg caaacaggac gacaatccag actaccgttt tcatgcgcgt
    38041 tctcctttag cggacggcga acgccctgcc ttttccggca cggactgttt ttttgcaggc
    38101 tgttccggtt tgcgttccga aggcagggac ggtgcttcta tggtcttcac atccgatgcg
    38161 gcagccggtt cggaagctgt gttttcgttt gccggttcgg aagccgcctg ttccgcttct
    38221 tgattttccg ccgccgtcat gcgcgtaccg tcgcgatagg cgcggacggc atttaggctg
    38281 tttttcaaac cgtcatccgc cgtcatgcgc acatccaacg ccttcaattc cgccagttct
    38341 ttcagccacg actgcgtggc gggagacttg gcatcgaaat actgtctgac ggcggcttcg
    38401 gcattgttca aatcgccctg atagacttcg ccgttgcgct gcattaatgc aatgcgcgca
    38461 tccaaaaggc ggaggcgcag gttttcacgc acaaaatatg cctgttccgg tgaaagcagc
    38521 atggcatcgt tgttttccaa acggcggatt tcgaccagcc ccttcaatgt gccgagggat
    38581 ttttcccata cgttctgcca ccatgaagcg gaaacggctt cgttgcgtgc ctgtacgccc
    38641 ggtttcaata cgccgtccaa aatcagcggc agtccggata cggcggtttc cagcctgtcg
    38701 aggcgcaatg ccgtgccgga aatatcgaca tagggacggt ttttcagttc cgccaagtcg
    38761 ctgctgactg cctgtttgat cggcagaagc tctgcctgat tgaaacggga caggcggctg
    38821 tcgatatgct ccaacacgcc gactgccgtt tggatattgc cggtcaacac cagctgttgc
    38881 gccgccagat tgagtatggt ctcggtttcg tccaccagcc aatcggcacg ccctttggtc
    38941 agctcgcgat aggctttttg cgtcatcaga atctgttcgc cgttggcttt gacaccgctg
    39001 tccaaacggt cgagttcgga ctgtatggca ctttggcggt tgaggttgtc tttgagcagg
    39061 gcggcgtttt ccgactcgcc caaagcggct ttgtcgattt tttggttgaa tgccaactct
    39121 tggtttttca agacattctg tccttggaca aacaaaaaac cacttgtacc caaacccaat
    39181 gccgccaata ccagggcgca gactgccaaa gcgttgctgc cggactgttt gacgataaaa
    39241 ggagcggcag gaatgtgtac ttctgtttcg ttttctttgc gtggggaaga ggtttcagac
    39301 ggcatttctt ttgatgcctg tatctcgcgt acgggttcgg atgatttgtt ttcaggttcg
    39361 cccaccgttt tgctccttat taatttgagg ttccgggaaa gaccatgccg tctgaaacgg
    39421 aaatggaaga atgggaaagc gcggcttcca gcgtagggac ggtttcgacc gaacacacgc
    39481 cttcgcgctt caatgcctcc gcaatgcgcg gatgatgggt aaagtatagc aaggatttga
    39541 agaatcggga aaattgcggc ggaagctgcc cgaacagcaa gcgcaccagc tcggtcgacg
    39601 taatataggc ggcggcaata ttttcggttt ggaaattttg aaagttcaaa ggtttatgcc
    39661 gtctgaaata gacttctgcc atctccgtcc gaaaaccttt ctcctgcaag gcattcatca
    39721 gaaaatcccg cccgccgtgt ccgcgcacaa acaatacgcg tgcaccttcg ggcagactgt
    39781 tccaaaccgg caggcgcaaa accgcctcgc tgtcgtttcc gtcatgaggc gcaatgaccg
    39841 ttctgcccaa atagcgttcc aatgcgcggc ggctgccctg ccctacggca atgtgtatct
    39901 ttatgccgtc tgaaaggtta aggtacggga cggcggtttc aacggcggtc ggactgaccc
    39961 aaaacacggc atccgcacgg gcatattgtt cggacaaaag ttccagtccg gcagcatctg
    40021 cttcgatttc aatcgggctc aacacttccg cctgccatcc ggcacgacgg caggtttcca
    40081 catcgtcctt cgccctgccc gacggtcgga caatcagcat gatgggtttc gctgtttcca
    40141 tcgccttatc cgccccgatt accgggaaaa atcagcatcg ttgaaattcg cctgcgcggt
    40201 ttgcggcgta cagccgaccc gtgcgacttc cttgccccta cgctccacaa cgacaccgct
    40261 gctttccgta tgagtcacgg aatcgaagcc tgtccatatg gtgtacataa attcgccgtt
    40321 ttggaatttc atcgttgccc aacgaccgct gcccatacct tgccacctgt cggaacgccc
    40381 cgacagatca gctttgctgt tgcgtatggc aatttctttt tttgccgcac tgccgaacga
    40441 atattcgtaa agattgcggt tgattttttg aacttctata tatttgttgt tgtccgtttt
    40501 acaggaaaac accgtatccg cccacaccgt ttgggcggca gccgacagaa gcaatacggc
    40561 agaaaggaac atttttttca tcgtatttcc ttaatggttg aaaccccgcc actcggacat
    40621 ccgtccttcg tggcagcagc atcagatttt atttgagagg ggtgtaacct cttccaaatt
    40681 agggcaacac atagggcaac gctttatgtg tcgtcctgtg tgttgaaaca tcattgcccg
    40741 tatcctgaaa tagaaaagaa aatgcccgtt ttacggtctt ctttccgaca aaatgccgtc
    40801 tgaaacggat ttcagacggc atggtcgaaa attattgcgc ggcaagcggc ttgagcagca
    40861 aggtttcgcc gctttgcaca actttttcaa gcaggtcgat attgggatgc ttgccgggat
    40921 gggcgcgcgc gtcggcaacg tgttcggcaa accatatcaa accctgttct gccgcttctt
    40981 tgtccaattt tccgtcaaaa cgttctgcca aagcgttgta caacttgagc gaaccgagct
    41041 taccctgaac ggcaggaatg tggtgaaccg ttttgccatc tgcatcctga acgtccaaac
    41101 cgcccagatg gtcgattgaa ggaaatgtgg cgagatagtc ttgaaaattc atatcaactc
    41161 cgaaaatcag tcagttagcg cgtgccgaaa atcttgtcgc ccgcatcgcc caagccgggg
    41221 atgatgtagc cgttctcgtt caagtggctg tcgagcgcgg cggtgtaaat cgtaacgtcg
    41281 ggatgcgcgt cgttgaccgc cttcacaccc tcgggcgcgg caaccagcac cagtgccttg
    41341 atgtttttgc agcccttggc tttcaaaagg tcgatggtgg caaccatcga accgcccgtc
    41401 gccagcatcg ggtcgataat cagggcagga cgttcgtcca tgctgtccac gaatttctcg
    41461 aagtaggaaa cgggtttcag cgtttcctcg tcgcgctgca gtccgactac gctgattttg
    41521 gcagtcggaa tcaggtcgag tacgccgtca agcataccca aaccggcacg caggatggga
    41581 acgacggtca atgttttgcc cttgatgcgg tcgccttcaa tctgaccgca ccatccgtcg
    41641 ataaggtatt tttcgatttc aaaatcacga cttgcttcgt acgccatcag gcgcgccagc
    41701 tctgtggtaa gcgttcggaa tttgtaggtg ctgcaatctg cctccctcat cagggttaat
    41761 ttgtggcgga cgagcggatg gttgataacg ttaacgttca tcgcggtgtt ccagtgtgat
    41821 tttgagacgc gccattatac ttttttttga tttgccggaa aagaaaatgc cgtctgaaac
    41881 acgttcagac ggcattgccg aatccatcag gctttcagca gctcttgcag ctcgcctgcc
    41941 tcatacattt ccatcaggat gtccgaaccg ccgacaaact cgccgttcac ataaagttgg
    42001 gggatggtcg gccagtcgct gtattcttta ataccttggc gtacttcggg attttccaat
    42061 acgttgacgg taacgtaatc ggtgcagcct gccgcgttca ggatttgcac ggcgcgggaa
    42121 gagaaaccgc attgcggaaa ctgcttcgta cctttcataa acaatacgac gcggtgtgtc
    42181 gttactactt ctttaatttg gtcgtggatg gaagccatcg cttattcctt ttgataaagt
    42241 tgacaaaatt tgtcaggtag gacgcattat acgcaaaagc ggaaacttcc cacaatattt
    42301 gccgtatatc gcgcagtatc aatgttgtga acggaaatat tcccactcct cgacaatcct
    42361 gctgtccggc aaatggcaca gggcatatcc gccgccgctt tcgtcttttt ttacttcccg
    42421 tctgccgcct ttggcaatgc agaatttgga agcggaattt gccatgcctg tccgaaacgt
    42481 gttggcaggc agccggcagc agcgcggaca tgcatacgga tgaattgcct tcatggtttt
    42541 ccttatcgtc ggtacagggt cagacgaccc gcatatcggg tactttttta aacagccggt
    42601 aaaagttttc cgtcgtatat gccgcaacct gttccaatgt ttggttccgc aatttggcga
    42661 tatgttcggc ggtatagcgc acaaaagccg gttcgttctg cctgccgcgt ttgggaacgg
    42721 gggcgaggaa cggcgcatcg gtttccacca aaatgcggtc gtccggcaca tatttcgccg
    42781 cctcctgaac caagggggcg tttttaaagg taacgattcc cgagaaagaa atataaagcc
    42841 ccaaatccat ggcagcccgg gcaaaggctg tgtcttcgga aaaacagtgg ataacgcccg
    42901 aattaacccg gcattctttc aggatagaca aggtgtccgc cgccgcatca cgcgtatgga
    42961 cgataacggg cagtccggtt tgattggctg cttcgatgtg gtctgcaaag cgtttgtgtt
    43021 gccaggacaa atcgcctttg caccagtaat aatccaagcc cgtctcgcca atgccgacca
    43081 ctttcggatg ggcggctgca gcaaccattt ccgcaataga aaattcttcg gcttccttgc
    43141 tgtcgggatg tacgcctatg gtgcaataga tgtgttcgtg cgcttcggcg atggcaaaga
    43201 cttcggagaa gctttcccta ctgacgctga tggcgagcac ctgccccacg ccgtttgctt
    43261 ccatgttgga caaaacttcg ggcaggcgtt ctttcaaacc ttcaaaattg aggtggcagt
    43321 gcgaatcgat gatatgcatg gcgttacatg gtaaacgtag gtctgctgcc gccgattgtg
    43381 ttgcccagtt cgacttcgat ctggtctttg actttcaggc agttttcgtg ttttgtgaat
    43441 gccagcccca cccctttggg tttggaggaa gtacgcgcag gattgatcca ggcgactttg
    43501 gtcggcagga acagtttggg gaagttgagg atttctacgg caagcagaat atcgtccccg
    43561 atggaaaata cgtcgttggt ctgcacaaac agaccgccgt gttccaaaaa cggcatatag
    43621 gagctgtaca gcagattcat gtctttcagc tgcaacgaca tcatttttgc cggaatattt
    43681 tgtccgtctg acataattca cccgttctct ttcttcaatt ccaaataatt gatgagcaga
    43741 tgctcgatct gcattttgac atttaaagta tgaaacccgt aaggggcaag ctgtttgagc
    43801 atatcctccg ccgcaaatac attgcgcggg cggaaaccgg atgccgtctg aagcagcctg
    43861 tcttcataag cgggataata gacgggtttc atgtgttgca ggcacaatcc caaatcgacc
    43921 agccatttct gcatccaccc gacaaatacg tcgagcggaa gtttctcctt atcgaaaagc
    43981 gcggcgtaat ccaaaatctt caacaacctt ggttctgcca aaatatccaa cagtttaatc
    44041 cgcaacgcac ggacaccgtc cgcctcatca aacagcggcg cacccgaatg gaacgccaaa
    44101 aattcttccg gctccgccac acccctgtcg cgcagatatg ccaatgcttc cccatgcgaa
    44161 ggagcgggca aaaccatctt ccggcagcga cttttaatgg tcggtaaaac cttgtccgcc
    44221 gcgtggctga ccagcaaaaa gaccacttgt ggcggcggtt cttccaacac tttcaacaaa
    44281 ctgttggcgg cttggacatt catactttcc gcaggatgaa tcagaatcac gcgcaaaccg
    44341 ccccgtaccg aagtcaggta cacattatcg atgatttccc tgacggcatc gattttgatc
    44401 tgcaacagtt tgcgtccgtt ttcgggttcg tccgacaagg gggtgatttc gtaaaaatcg
    44461 ggatggcttc cctgtccaaa cagatggcag gacatacatt cgccacaggg tttgcagccc
    44521 ggtgcagggg tttcgcacaa cagtgccttc gccgcaaagc gggcaaatgt agttttcccc
    44581 gtatcttttt tgccggcaaa cagccatgca ttgggacgac gctcccaatg ttccgcaatc
    44641 tgccgccatt gctcattatg ccacggataa atcatttcaa accatccgtc cggaaatcca
    44701 taccgccatc agagcagttc caaatcgggg ctggaacttt gcgcgcggcg gctgcgcaac
    44761 tgtaccgcca aacgcaaatc gtgtgcggaa tcggcatttt tcaatgcttc ttgcagggaa
    44821 atatcgcctt tttcatacaa ttggtaaagg tgttgatcga aggtctgcat acccagggta
    44881 gtggattttt tcatcacttc tttgatttca tggatgttgc cgttgtgaat caactccgaa
    44941 atcaggggcg aattgagcag cacctcgact gccgccaccc tgcccttgcc gccgtctcgc
    45001 ggaacgaggc gttgcgaaat aaacgcctga aggttgagcg acaaatccgt cagcaattgt
    45061 tcgcgccgct cctcggggaa aaagttgatg atgcggtcga gtgcctgatt ggtgctgttg
    45121 gcgtgcagcg tcgccataca caaatgcccc gtttcggcaa aggcaatggc gtagtccatc
    45181 gtttcgcggt cgcggatttc gccgataagg atgacatccg gcgcctgacg cagcgtgttt
    45241 ttcaacgccg ccatccagtt ttccgtatcc acgccgacct cgcgctgggt gatgatgcag
    45301 tttttgtgtt cgtggacaaa ctcgatcggg tcttcgatgg tgatgatatg tccgaacgaa
    45361 ttttcattgc ggtaatcgat aagcgaggca agcgaagtcg atttgcccga gccggtgccg
    45421 ccgacaaaaa taaccagccc gcgttttttc agcgcgacat ccttcaagac cggcggcagg
    45481 ttcaggcttt caaacttggg aatcttgctg gtaatcgcac ggaataccaa cgccgtcgcg
    45541 ccgcgctgta tcatcgcatt gacgcggaag cggctggtgt ccggcaggct gatggcgaag
    45601 ttgcactcgt tggtcgatga aaattcttcc gcctgcttcg cactcataat cgaaaaggcg
    45661 atttccatac atttttccgc cgtcagcggt tcgtccgtga tgcgggtgat tttgccgtcc
    45721 agcttcattg cgggcgggaa atgggtcgtc acaaacaggt cggaaccttt gtttttgttc
    45781 atatggcgca gccatgcgaa cagttcttcc tttgcggtca tattttcatc ggtaaacatc
    45841 ctcgtcccct aatcttcaaa caatctgtac cttcccattc taccaaaaac ccgccgtgcc
    45901 gtctgaaacc gtttcagacg gcacggacaa accacctcca ctcaatcctg ccgtgttagc
    45961 gtgtataatc aggcattcta tccgaaattc acaacaggtc agcaatatgt ctttgaaaac
    46021 agtagccgtt atcggcgcaa tggaacaaga aatcgagctt ttgcgcgaga tgatggaaaa
    46081 tgtcaaagcc gtctctttcg gcaagtttgc cgcctatgaa ggcgaattag cgggaaaacg
    46141 catggtgctt gcattgagcg gcatcggcaa ggtcaacgcg gcggttgcaa cggcttggct
    46201 tatccatcaa ttcgcaccgg actgcgttat caacaccggc agcgcgggcg gcttgggcaa
    46261 gggtttgaaa gtcggcgatg tcgtggtcgg cacagaaatc gcgcaccacg atgtcgatgt
    46321 aaccgcattc ggctatgttt ggggacaagt gccgcaactg cccgccgtat ttgtttcaga
    46381 cggcctcttg gtcggcaaag caaaacaggc ggcggaagtg tttgaaggcg cggcggtaga
    46441 acaaggcctg attgtcagcg gcgaccgctt cgtccacagc agcgaaggcg tggcggaaat
    46501 ccgcaagcac ttccccaaag tcaaagcagt ggaaatggaa gcggcggcga ttgcccaaac
    46561 ctgtcatcag ttggaaacgc ctttcgtcat catccgcgcg gtttccgatt cggcggacga
    46621 aaaggcagac atcagctttg acgaattttt gaaaacggcg gcggcaagtt ctgcaaaaat
    46681 ggtggcagaa atcgtcaaat ctttataaaa cctttatcaa atcatgccaa aacagcggtt
    46741 ttccgttaga atatcgggct atttccacgt tgcacccgat attcggacgc aacgccgttt
    46801 gttcagacgg catttcccaa acccactgtc cgacattcaa aacaactcta atgaaaaata
    46861 tccgaaattt ctccatcatt gcccacatcg accacggcaa atcgacgctt gccgaccgct
    46921 tcatccaata ttgcggcggt ttggatttgc gcgaaatgag tacgcaggta ctcgattcca
    46981 tggacatcga aaaagagcgc ggcattacca tcaaagcgca aaccgccgcg ctcaactata
    47041 aagcgcgcga cgggcaggtg tatcagctta atttgattga cacgccggga cacgtcgact
    47101 tctcttacga agtttcccgt tcgctgtcgg cttgcgaagg cgcgcttctg gtcgttgacg
    47161 cgtcgcaggg cgtggaagcg caaaccgtag cgaactgtta caccgcgatt gatttgggcg
    47221 tggaggttgt gcctgtttta aacaaaatcg acctgcccgc cgccgacccc gagcgtgtgg
    47281 aacaggaaat cgaagacatc atcggcatcg atgccgtcgg tgcagtacag tgttccgcca
    47341 aaagcggtat cggcgtggaa gatgttttgg aagaaattgt tgccaaaatc cctgcgccga
    47401 ccggcgacga aaacgcgccg ctgcaagcgg ttatcgtcga ttcgtggttt gacaactatg
    47461 tcggcgtggt catgctgatt cgagtgaaaa acggcaccat caaactgaaa gacaaagtgc
    47521 gctttataag caccaaggcg gaaacgcagg tcgagcagct gggcgtattc acaccaaaat
    47581 cagttcaaaa acaagaactc aaagccggcg aagtgggctt tttgattacc ggcgtaaaag
    47641 aattgggtca ggcgaaagtc ggcgatacgg ttacactggt tgccaactct gccactgagc
    47701 cgctgcccgg cttccaagaa gtacaaagcc aagtattcgc cggactttat cccgtggaaa
    47761 gccacgacta cgaagccttg cgcgacgctt tggaaaaatt gcagcttaac gacgcttcgt
    47821 tgaaattcga gcctgaagtt tcccaggcat tgggcttcgg cttccgttgc ggcttcttgg
    47881 gtctgttgca cttggaaatc gtgcaggaac gcttggagcg cgagttcgat atggatttga
    47941 ttaccaccgc gccgacggtg gtttatgaag tcgtgttgaa aaacggtgag aaaatcgaag
    48001 ttgaaaaccc gtccaaactg cccgacatcg gcagcatcga aaccatactc gagccgatta
    48061 ttaccgcgac catccttgtg ccgcaggaat acgtcggcaa cgtcatgact ttgtgtaacc
    48121 aaaagcgcgg cgttcaggtc aatatgcagt acatgggccg ccaagtgatg ctgacttacg
    48181 atttgcccat gaacgaagtc gtcatggact tcttcgacaa actcaaatcc acttcgcgcg
    48241 gttatgcttc gttggactac catttcaaag agttccaacc gtctgatttg attaagctgg
    48301 atattatggt caacggcgaa aaagtcgatg ccttaagcct gattgtgcac cgtcaaagcg
    48361 cggttcaccg aggccgcgag ctggcatcga aaatgcgcga gctgattccg cgccaaatgt
    48421 tcgacattgc cgtccaagcc gccatcggca gccagattat cgcccgcgaa aacgtcaaag
    48481 cactgcgtaa aaacgtctta gcgaaatgtt acggcggcga tattacgcgt aagaaaaaac
    48541 tgcttgaaaa acaaaaggca ggtaaacgcc gtatgaaaca agtgggcaat gtggaaattc
    48601 cgcaaagcgc gttcttggcg attttgcagg tgagcgacaa ataactctgg cacacagggc
    48661 ggcgcgtcgg gaacacccct caaaccgccc tgatttcagg ggcacgcgcc cgcaaacaaa
    48721 ttttgcaatt tgccgcctta aaacggttgc acggacgggc ggttttttaa gcattaagga
    48781 ataacaatga acactatgct aatgtcgggc gcggctgccg cgctgcttgc cggcatcatc
    48841 ctttatttca aaagcgacaa aaagcggcag gaaaacggtg aatggagttc cggcctcgaa
    48901 tacgcctata tcctgacagc ggtcggcgtg tttgccgctt tgtccctgtt tatgagcttt
    48961 accgccgttt tcctgatttt cgttgtattg tgcggtacgg cttggggggt atataaatac
    49021 cgcctgaaga ctcatcccga aatctcggaa agcagccact tcggcgatta tttcggcagt
    49081 ttcttcccta ccgttttggt attgttcctc atccggtcgt ttatcgccga accgttccaa
    49141 atcccgtcca gctcgatgcg cccgggcctg atcaaaggcg atttcatttt ggtcggcaaa
    49201 ttttcctacg gcctgcgcgt acccgtttta aacaatgtat tcatccccac gggcaaaatc
    49261 gaacggggcg atgtggtcgt ttttaattat cctttgcaac cggagatgac ctacatcaag
    49321 cgtattgtcg gcattccggg cgatgtggtc gaatatcgga ataaagtttt gacggtaaac
    49381 ggaaaacctg cttccgatat tcctgacggc acataccgtt atcccgacga caccgaccca
    49441 tccgaaatcc acaatacgga tatgttccac agcggtttgg acggcaaatc cttcaatatt
    49501 ctgaaaaaag aaggacagcc tgccgtttcc ctgcccgtat tgggcaaata tacctccgat
    49561 attatgtctg aaaacggata ttccatagag caaagcggtt tggaacactg ccaatatgcc
    49621 gacgacggca gcggtttcgt gtgcaaagtt cccgaaggac gctatttcgc tatgggcgac
    49681 aaccgcgaca acagtgccga ttctcgctac tggggatttg tggatgacaa gctggttgtc
    49741 ggcaaggcaa tgttcatttt gatgaacttc ggcgatttcg gcagggccgg tacggcaatc
    49801 cgttaggcca ggaagccaag aatgccgtct gaaaatagtt tcagacggca tttgctattc
    49861 ctcacttttg caggcggatc taacgttgtc ggattttcct ac
//
LOCUS       DAWGJV010000002        39329 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWGJV010000002 DAWGJV010000000
VERSION     DAWGJV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.002"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            789..2933
                     /locus_tag="WHQ94_000055"
     CDS             789..2933
                     /locus_tag="WHQ94_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2127411.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKAVLDTITVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNNFNGGK
                     TVPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGGEDSRINIG
                     RQAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPN
                     HTQITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKKDATEEVKK
                     KNRANENIAKAYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSS
                     SNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARN
                     TEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGYELGAS
                     YRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIG
                     WRGRYVQKAEGSILVSGQKDRQGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNN
                     VFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            3084..4049
                     /locus_tag="WHQ94_000056"
     CDS             3084..4049
                     /locus_tag="WHQ94_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2127412.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQTEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNLLPYLDEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKRQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            4067..4216
                     /locus_tag="WHQ94_000057"
     CDS             4067..4216
                     /locus_tag="WHQ94_000057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127413.1"
                     /translation="MPSETRMQTASCVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            4213..5181
                     /locus_tag="WHQ94_000058"
     CDS             4213..5181
                     /locus_tag="WHQ94_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2127414.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADQLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            5171..6145
                     /locus_tag="WHQ94_000059"
     CDS             5171..6145
                     /locus_tag="WHQ94_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ2127415.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLLVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCIGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            6190..6816
                     /locus_tag="WHQ94_000060"
     CDS             6190..6816
                     /locus_tag="WHQ94_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127416.1"
                     /translation="MTQERFPSFFNQAPTITVRDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            6900..7649
                     /locus_tag="WHQ94_000061"
     CDS             6900..7649
                     /locus_tag="WHQ94_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2127417.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLAHGNISYAGKDTPTAELACTLSILTQENSIMSRITVRDLLMFGRYPYHQ
                     GRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLDEPL
                     NNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQGKPD
                     DIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(7708..8046)
                     /locus_tag="WHQ94_000062"
     CDS             complement(7708..8046)
                     /locus_tag="WHQ94_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ2127418.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            8141..8353
                     /locus_tag="WHQ94_000063"
     CDS             8141..8353
                     /locus_tag="WHQ94_000063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127419.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            8511..12497
                     /gene="purL"
                     /locus_tag="WHQ94_000064"
     CDS             8511..12497
                     /gene="purL"
                     /locus_tag="WHQ94_000064"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ2127420.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALNRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQAYGKPDHIASPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATGDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCIGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADK
                     KSVVSPLSLIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            12592..13350
                     /gene="gloB"
                     /locus_tag="WHQ94_000065"
     CDS             12592..13350
                     /gene="gloB"
                     /locus_tag="WHQ94_000065"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ2127421.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            13664..18136
                     /gene="mspA"
                     /locus_tag="WHQ94_000066"
                     /gene_synonym="ausI"
     CDS             13664..18136
                     /gene="mspA"
                     /locus_tag="WHQ94_000066"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ2127422.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNLEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPLKLEGDSRGAFQIH
                     VKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPL
                     KEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARID
                     SQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELDT
                     YIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLA
                     ELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRP
                     YQQTTNYAHISIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGA
                     LFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNG
                     NRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYDTRQNSGSALSV
                     NDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            18368..19822
                     /gene="mgtE"
                     /locus_tag="WHQ94_000067"
     CDS             18368..19822
                     /gene="mgtE"
                     /locus_tag="WHQ94_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ2127423.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(19908..20816)
                     /gene="hslO"
                     /locus_tag="WHQ94_000068"
     CDS             complement(19908..20816)
                     /gene="hslO"
                     /locus_tag="WHQ94_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ2127424.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            21061..21816
                     /locus_tag="WHQ94_000069"
     CDS             21061..21816
                     /locus_tag="WHQ94_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ2127425.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            21835..22053
                     /locus_tag="WHQ94_000070"
     CDS             21835..22053
                     /locus_tag="WHQ94_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127426.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            22198..22542
                     /locus_tag="WHQ94_000071"
     CDS             22198..22542
                     /locus_tag="WHQ94_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ2127427.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            22542..23234
                     /locus_tag="WHQ94_000072"
     CDS             22542..23234
                     /locus_tag="WHQ94_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ2127428.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            23301..24521
                     /gene="argJ"
                     /locus_tag="WHQ94_000073"
     CDS             23301..24521
                     /gene="argJ"
                     /locus_tag="WHQ94_000073"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ2127429.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            24581..25990
                     /locus_tag="WHQ94_000074"
     CDS             24581..25990
                     /locus_tag="WHQ94_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ2127430.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGVSIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLTSGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            26180..27571
                     /gene="hrpA"
                     /locus_tag="WHQ94_000075"
     CDS             26180..27571
                     /gene="hrpA"
                     /locus_tag="WHQ94_000075"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ2127431.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            27672..28799
                     /locus_tag="WHQ94_000076"
     CDS             27672..28799
                     /locus_tag="WHQ94_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127432.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            28976..30553
                     /gene="lpt3"
                     /locus_tag="WHQ94_000077"
     CDS             28976..30553
                     /gene="lpt3"
                     /locus_tag="WHQ94_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ2127433.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            30655..33864
                     /locus_tag="WHQ94_000078"
     CDS             30655..33864
                     /locus_tag="WHQ94_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ2127434.1"
                     /translation="MDARSNPANVSDGLQNSSSHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAASKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRSPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTSTRGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAAIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVIASERVTLYGLTVLPRRPVSYGRIAPEEAR
                     EIFIRGALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGESKTVATQTNFSATSTLSDDPKPKKQPA
                     PQKGRLKPLPLADIRTFQAWLKTAECDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPK
                     QIRRICVPVPDFITKFLESNPDRQATIIPQLAHFIAKSASDMRILEQIDQDAWAAQEL
                     PEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKNDGLVKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLNK"
     gene            33923..35530
                     /locus_tag="WHQ94_000079"
     CDS             33923..35530
                     /locus_tag="WHQ94_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2127435.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNTIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPFKFKPNVLF
                     HKGDKKQSINDRLIWSLSRSVMEINKALLDVLKLVTKKHAEHQQFLQSPFITQDDIDI
                     AVSGILDQIDKLNLRIKDCERLESLCR"
     gene            35521..36981
                     /locus_tag="WHQ94_000080"
     CDS             35521..36981
                     /locus_tag="WHQ94_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ2127436.1"
                     /translation="MSLNSKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQTKLALLQKNPFVLHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPDKQNLPLKLLTEKWQQQLDYAKSQWYLEQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYIQNPQIDINSREEIIGLRLGKDLEYVLPSELALMADEETSILFDLK
                     FLESKLMCFELQGTTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQESYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(37335..37808)
                     /locus_tag="WHQ94_000081"
                     /pseudo
     CDS             complement(37335..37808)
                     /locus_tag="WHQ94_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            38040..38252
                     /locus_tag="WHQ94_000082"
     CDS             38040..38252
                     /locus_tag="WHQ94_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127437.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            38549..38704
                     /locus_tag="WHQ94_000083"
     CDS             38549..38704
                     /locus_tag="WHQ94_000083"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ2127438.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(38794..39243)
                     /locus_tag="WHQ94_000084"
     CDS             complement(38794..39243)
                     /locus_tag="WHQ94_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ2127439.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFILKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT        10095 a        11411 c         9391 g         8432 t
ORIGIN      
        1 cgttcgtttt cggttattcc cgataaatta ccgccgtttc tcatcatttc tttaaccctt
       61 cgtcattccc gcgcaggcgg gaatctagtt tttttgagtt ccagttgttt ctgataaatt
      121 cttgcagctt tgagttccta gattcccgct ttcgcgggaa ggacgcggaa aggttgcttt
      181 tcgtcattcc cgcgcaggct tcgtcattcc cgcgcaggct tcgtcattcc cgcgcaggcg
      241 ggaatccagt ctgttcggtt tcggtttttt ccgatagatt cctgcccgtt aggggttcta
      301 gattcccgct ttcgcgggaa ggacgcggaa aggttgcttt tcgtcattcc cgcgcaggcg
      361 ggaatctagg ttcgttcggt ttcggttttt tccgatagat tcctgccgcg ttaggggttc
      421 tagattcccg ctttcgcggg aaggacgcgg aaaggttgct tttcgtcatt cccgcatttg
      481 ttaatccact atatttccgc cgttttttat agtggactaa caaaaaccag tacagcgttg
      541 cctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttatcc tgatttttgt
      601 tagtccacta tacatttccg acaaaacctg tcaacaaaaa acaacacttc gcaaataaaa
      661 acgataatca gctttacaca aatcccccct ctcgttaata taaacaaaaa taattattat
      721 ttttcttatc ctgccaaacc ttaacggttt ggatttactt cccttcatac actcaaaagg
      781 acgaacaaat gaacgccccg tttttccgcc tcagcctgct ctcgcttacc ctggcggcag
      841 gttttgccca cgcggcagaa aataatgcca aggccgtact ggataccatt accgtaaaag
      901 gcgaccgcca aggcagcaaa atccgtacca acatcgttac gcttcaacaa aaagacgaaa
      961 gcaccgcaac cgatatgcgc gaactcttaa aagaagagcc gtccatcgat ttcggcggcg
     1021 gcaacggcac gtcccaattc ctgacgctgc gcggcatggg tcagaactct gtcgacatca
     1081 aggtggacaa cgcctattcc gacagccaaa tcctttacca ccaaggcaga tttattgtcg
     1141 atcccgcttt ggttaaagtc gtttccgtac aaaaaggcgc gggttccgcc tctgccggta
     1201 tcggcgcgac caacggcgcg attatcgcca aaaccgtcga tgcccaagac ctgctcaaag
     1261 gcttggataa aaactggggc gtgcgcctca acagcggctt tgccagcaac gaaggcgtaa
     1321 gctacggcgc cagcgtattc ggaaaagagg gcaacttcga cggtttgttc tcttacaacc
     1381 gcaacgatga aaaagattac gaagcaggta aaggcttccg taataatttc aacggcggca
     1441 aaaccgtacc gtacagcgcg ctggacaaac gcagctacct cgccaaaatc ggaacaagct
     1501 tcggcgacgg cgaccaccgc atcgtgttga gccatatgaa agaccaacac cggggcatcc
     1561 gcactgtgcg tgaagaattt accgtcggcg gcgaagattc acggataaat attggccgcc
     1621 aagcccctgc ttaccgcgaa accacccaat ccaacaccaa tttggcgtac accggcaaag
     1681 atttgggctt tgtcgaaaaa ctggatgcca acgcctatgt gttggaaaaa gaacgctatt
     1741 ccgccgatga cagcggcacc ggctacgcag gcaatgtaaa aggccccaac catacccaaa
     1801 tcaccactcg gggtatgaat ttcaacttcg acagccgcct tgccgaacaa accctgctga
     1861 aatacggtat caactaccgc catcaggaaa tcaaaccgca agcgtttttg aattcacaat
     1921 ttaaaattga agataaaaaa gatgcaactg aggaagtgaa aaagaagaac cgtgccaatg
     1981 aaaacattgc caaagcctac cgtctgacca acccgaccaa aaccgatacc ggcgcgtata
     2041 tcgaagccat tcacgagatt gacggtttta ccctgaccgg cgggctgcgt tacgaccgct
     2101 tcaaggtgaa aacccacgac ggcaaaaccg tttcaagcag caaccttaac ccgagtttcg
     2161 gcgtgatttg gcagccgcac gaacactgga gcttcagcgc gagccacaac tacgccagcc
     2221 gcagcccgcg cctgtatgac gcgctgcaaa cccacggtaa acgcggcatc atctcgattg
     2281 ccgacggcac gaaagccgaa cgcgcgcgca ataccgaaat cggcttcaac tacaacgacg
     2341 gcacgtttgc cgccaacggc agctacttct ggcaaaccat caaagacgcg cttgccaatc
     2401 cgcaaaaccg ccacgtctct gccgctgtcc gtgaagccgt caatgccggt tacatcaaaa
     2461 accacggtta cgaattgggc gcgtcctacc gcaccggcgg cctgactgcc aaagtcggcg
     2521 taagccacag caaaccgcgc ttttacgata cgcacaaaga caagctgttg agcgcgaatc
     2581 ctgaatttgg cgcacaagtc ggccgcactt ggacagcctc ccttgcctac cgcttcaaaa
     2641 acccgaatct ggaaatcggc tggcgcggac gctatgttca aaaagctgag ggttcgatat
     2701 tggtgtcagg tcaaaaagac cgccaaggca aactggaaaa cgttgtacgc caaggtttcg
     2761 gtgtgaacga tgtcttcgcc aactggaaac cgctgggcaa agacacgctc aatgttaatc
     2821 tttcggttaa caacgtgttc aacacgttct actatccgca cagccaacgc tggaccaata
     2881 ccctgccggg cgtgggccgc gatgtacgct tgggcgtgaa ctacaagttc taaaacgcac
     2941 atcccgaaaa aatgccgtct gaaagccttt cagacggcat ctgttctgat aatttgatat
     3001 aggttatcat ttatcctttc taaagccgtt ccggtttgtc cgaccggcgg ctttgcccca
     3061 atatccccat tttggagaca cctatgttac gtttgaccgc attgaccctc tgcaccgtcc
     3121 tcgccttggg cgcgtgttcg ccgcaaaatt ccgactctgc cccacaagcc aaagaacagg
     3181 cggtttccgc cgcacaaacc gaaggcgcgt ccgttaccgt caaaaccgca cgcggcgatg
     3241 ttcaaatacc gcaaaacccc gaacgcatcg ccgtttacga tttgggtatg ctcgacacct
     3301 tgagcaaact gggcgtgaaa accggtttgt ccgtcgataa aaacctgctg ccttatttgg
     3361 acgaatattt caaaaccacg aaacctgccg gtacgctgtt tgagccggat tacgaaacgc
     3421 tcaacgctta caaaccgcag ctcatcatca tcggcagccg cgcagccaag gcgtttgaca
     3481 aattgaacga aatcgcgccg accatcgaaa tgaccgctga taccgccaac ctcaaagaaa
     3541 gtgccaaaga gcgtatcgac gcgctggcgc aaatcttcgg caaacaggcg gaagccgaca
     3601 agctgaaggc ggaaatcgac gcgtcttttg aagccgcgaa aactgccgcg caaggcaaag
     3661 gcaaaggttt ggtgattttg gtcaacggcg gcaagatgtc cgccttcggc ccgtcttcac
     3721 gactgggcgg ctggctgcac aaagacatcg gcgttcccgc tgttgacgaa gccatcaaag
     3781 aaggcagcca cggtcagcct atcagctttg aatacctgaa agagaaaaat cccgactggc
     3841 tgtttgtcct tgaccgcagc gcggccatcg gcgaagaggg tcaggcggcg aaagacgtgt
     3901 tgaataatcc gctggttgcc gaaacaaccg cctggaaaaa aagacaagtc gtttacctcg
     3961 ttcctgaaac ttatttggcg gccggcggcg cgcaagagct gctgaatgca agcaaacagg
     4021 ttgcggatgc ttttaacgcc gcgaaataat gaaacggcgg cattcgatgc cgtctgaaac
     4081 acggatgcaa accgcttcct gtgtttcaga cggcattgcc cgatacggag gcttcaaaca
     4141 aggctttccg ctccgacggt tcggactgcc ttgtttgaat cttctacgcc ttaacgcttt
     4201 tcccttctgt ttatgactgc caaacctttt tccctcaacc tgaccaacct gctgctgctg
     4261 gcggtgttgt ttgccgtcag cctgtcggtg ggcgttgccg atttccgctg gtctgatgtg
     4321 ttttcactgt ccgacagcca gcaggtcatg ttcatcagcc gcctgccgcg cacgtttgcg
     4381 attgtgctga cgggcgcgtc gatggcggtg gcggggatga ttatgcagat tttgatgcgc
     4441 aaccgttttg tcgagccttc tatggcgggc gcgggtcaga gtgcggcttt gggtttgctt
     4501 ctgatgtccc tgctgctgcc tgccgcgccg ctgccggtca aaatgtcggt tgccgccgtt
     4561 gccgcgttaa tcgggatgtt ggtgtttatg atgcttatcc gccgcctgcc gccgacggcg
     4621 caactgatgg tgcctttggt cgggattatt ttcggcggcg tggttgaggc ggtggccacc
     4681 tttattgcgt atgaaaacga aatgctgcaa atgctgggcg tgtggcaaca gggcgatttt
     4741 tccggcgtgt tgctcggacg gtatgaactg ttgtgggcaa cggggatttt ggctttgttt
     4801 gcctatttga ttgccgacca gctgacgatt ttgggtttgg gcgaaacggt aagcgtgaac
     4861 ttggggctga accggacggc gattctgtgg tcggggctga ttattgtggc tttgattacg
     4921 tcgctggtta tcgttacggt cggcaatatt ccgtttatcg ggctggtcgt gccgaacatc
     4981 atcagccgcc tgataggcga caggctgcgc caaagcctgc ctgcggtggc tttgttgggc
     5041 gcgtctttgg tgttgctgtg cgacattatc ggacgcgtga ttgtgtttcc gtttgaaatt
     5101 ccggtatcga ccgtcttcgg cgtattgggt acggcgttgt ttttatggct tttgttaagg
     5161 aaacctgctc atgccgtctg aaaaaaatat cggttttatg gcaggaagca gccgtccgtt
     5221 gtgggttgcc tttgcgctgt tgctggtttc ctgcatcctg tttatgacgc tcaacgtcaa
     5281 aggcgattgg gattttgtac tgcacctgcg gctgaccaag ctcgcggctt tacttttagt
     5341 cgcctatgcg gtcggcgtgt ccactcaact cttccaaacg ctgaccaaca atccgattct
     5401 gaccccttcg attttgggtt tcgattcgct gtatgtgttt ttgcagacct tgctggtgtt
     5461 tacgttcggc ggcgtgggct atgcttccct gccgttgacg ggcaaattcg gctttgaact
     5521 tgccgttatg atgggcggct cgctgctgct gttttacacg ctcatccgtc agggcggacg
     5581 cgatttgccg cgtatgattt taatcggcgt gattttcggg attttgttcc gcagcctgtc
     5641 gtcgctgctt tcgcgcatga tcgaccccga agaatttacg gcggcgcagg cgaatatgtt
     5701 tgccggattc aataccgtcc acagcgagct tttgggcata ggcgcgctga ttctgctcgt
     5761 cagcgcggcg gtcgtttggc gcgaacgcta ccgcttggac gtacaccttt tggggcgcga
     5821 ccaagccgtc aatttgggca tcagctacac gcgcaacacc ttatggatac tgctttggat
     5881 tgccgcgctg gtggcgacgg cgaccgccgt ggtcggcccg gtaagctttt tcgggcttct
     5941 cgccgcctcg cttgccaacc acttttcccc gtccgtgcgc cattccgtcc gcctgccgat
     6001 gacggtttgt atcggcggca tcctcctggt cggcggacag accgtattcg aacacttctt
     6061 gggcatgaag gcggtattaa gcgtggtggt cgaatttgcg ggcggactcg ttttcctcta
     6121 tctcgtttta aaacacaaaa aatgacggat gccgtctgaa cggcagcctg gctcgaaagg
     6181 acaaaccata tgacgcaaga acgtttccca tcattcttca accaagcccc gaccatcacc
     6241 gtcagagacc cgcttgccgc attcctcggc gcggccgaaa acggcatcct cacttaccgc
     6301 tacgccgatg ccgtgcgcct gtgcggtcac tcctgcccga ccgtcgcggg cgcgtacctg
     6361 atggttatca aaggtctgaa agccctttac ggcgaagagc tgcccgaacg cggcggcatc
     6421 gaagccgcca tgcagggaac gcgcgacgaa ggcacggtcg gcgtaaccgc gtccgtcgtc
     6481 caactcctca ccggcgcagc ccccgaaacc ggcttcggag gcatcggaat gcagggacgc
     6541 ttcgcccgcc gcaacctctt atcctttggt gcgggcgaaa tcaacggcac actgaccctg
     6601 cgccgcaaag acaacggcaa aaccgtcgcc gtcagcctca acgccgccct gcaacccttc
     6661 gcacccgaaa tgcgcgaact tatgcccaaa gccgtcggtg gcagcgcaag cgcagaagaa
     6721 ctcgaacgct tcggacaact ctggcaggca cgcgttaaag catttttgac cgaatcggcg
     6781 gacgacccgc agttcgtcat cgtccgcgaa gtgtgagcgt tcagacggca ttccaaactt
     6841 caaatgccgt ctgaaccccc gccaaacaac aacaaaccta cgcccgacaa gcatccgcca
     6901 tgattaccat ccgcaacgtc agctaccgca tcggcacacg ccccatcctc gacaacgtca
     6961 gcctcgacat ccccgaaggc ggcattaccg cactgatcgg ccccaacggc gcaggcaagt
     7021 ccaccctcct ctcctttatg gcgcggcttc agcctctggc acacggcaac atcagctacg
     7081 caggcaaaga cacccccacc gccgaactcg cctgcacgct ctccatcctc acccaagaaa
     7141 acagcatcat gagccgcatc accgtgcgcg acctgctcat gttcggccgc tatccctacc
     7201 atcaaggcag accgactgcc gaatgccgcc gtatcgttaa cggtgcaatc gaagaattcc
     7261 acctgcaaga cttcgccgac cgctacctga ccgagctttc cggcggacaa cgccaacgcg
     7321 ccatgattgc catggtgttc tgccagcgca ccgactacgt tttgctggac gaaccgctga
     7381 acaacctcga catgtatcat gcccgctccc tcatgcaaat cctgcgccgg ctgaccgacg
     7441 aacacaagcg caccaccgtc gtcgtattac acgacatcaa ccaggccgcc gcctacgccg
     7501 accacgtcgt cgccatgaaa aacggccaag tcgccatgca gggcaagccc gacgacattt
     7561 tcaccgccgc aaacatcaaa accctgttcg atatggatgt gtccgtcctc gaccaccaaa
     7621 gcaaaaaact catcgtccac catatctgaa gctcaaaaag aaaatgccgt ctgaaaacct
     7681 ttcagacggc atttccattc caaacgctca aaccgccgcg tccgcacgtt cgcccgtgcg
     7741 gatacggatt gcctcctcga ccggcagcac aaaaatcttg ccgtcgccga tttttcccga
     7801 acgcgccacc tcgacaatca cgtcaatcgc gcgttccaca gcatcatccg ccaacaccag
     7861 ctcgattttg actttgggca ggaaatcgac ggcgtattcc gcaccgcgat agatttccgt
     7921 atgccccttc tgcctgccga accctttgac ctcgctgacg gtcatgcccg taatgccgat
     7981 ttccgtcaac gcctcgcgca cgtcgtcgag tttgaacggt ttgacaatcg cctcgatttt
     8041 tttcatcaca ttctccatta attaacgtaa acacacgaca aatatagcag aaaccgccgg
     8101 caaacgataa acggtactgt tcgcccggcg atgcggcgat ttgccggaaa ccgtcattcc
     8161 cgcgcaggcg ggaatcccga tctctgcatt tcagcaatat ttaaaggttt ctgtaaatcc
     8221 aaacttccat attcctaacc gtgcggaaac gacggcattt gatctaaaaa catacggaag
     8281 cagttgcccg cccctctact tcctgcaatt atccgcatac ccggctttgg gtaaaaatca
     8341 gaaaaacaga taaaaccctt attccttaat atcttcagac ggcacctgta ttttccccaa
     8401 ctgtatgccg tctgaagatt gtcaacattt taagccaaaa tatccaagca atacaaacct
     8461 gtccggcgta taatccctct ttttcccaac cgcatttgag agccgaatcc atgtctgtcg
     8521 ttttgccctt gcgcggcgtt accgcccttt ccgatttccg tgtcgaaaaa ctcttgcaaa
     8581 aagccgccgc actcggtctg cccgaagtca aattaagcag cgaattttgg tatttcgtcg
     8641 gcagcgagaa agcattggat gccgcgactg tcgaaaaact gcaagccttg ttggcggcgc
     8701 aaagcgttga acaaacgcca aaagcgcgcg agggcttgca tttgtttttg gtcacgcccc
     8761 gtttgggtac gatttcgccg tgggcttcca aggcgaccaa tatcgcggaa aactgcggtt
     8821 tggcaggcat cgaacgcatc gagcgcggca tggcggtgtg gctggaaggt gcgcttaccg
     8881 atgaacagaa acagcaatgg gcggcgttgc tgcacgaccg catgaccgaa tccgtattgc
     8941 ccgattttca gacggcatcc aaattattcc accatctcga atccgaaacc ttctctaccg
     9001 tcgatgtttt gggcggcggt aaagaggctt tagtcaaagc caataccgaa atgggtttgg
     9061 cactctccgc cgacgaaatc gattatctgg ttgaaaacta tcaggcttta aaccgtaatc
     9121 cgtccgatgt ggaactgatg atgttcgcgc aggcaaacag cgaacactgc cgccacaaaa
     9181 tcttcaacgc cgacttcatc ctcaacggcg aaaaacagcc gaaatccctc ttcggtatga
     9241 tacgcgacac gcacaacgcg catcccgaag gcacggtcgt cgcttataaa gacaattcgt
     9301 ccgtgattga aggcgcgaaa atcgagcgtt tctatccgga tgcggcggaa aaccaaggct
     9361 accgtttcca cgaggaagac acgcatatca tcatgaaagt ggaaacgcac aaccacccga
     9421 ccgccatcgc gccgtttgcg ggcgcggcga cgggcgcggg cggcgaaatc cgcgacgaag
     9481 gtgcgacggg caaaggttcg cgcccgaaag caggcttgac cggctttacc gtctccaacc
     9541 tcaacatccc cgacctcaaa cagccttggg aacaagccta cggcaaaccc gaccatattg
     9601 cttccccttt agacatcatg atcgaaggcc ccatcggtgg cgcggcgttc aacaacgaat
     9661 tcggacgccc caacctcttg ggctacttcc gcacctttga agaaaaattt gacggtcagg
     9721 ttcgcggcta ccacaaaccg attatgattg cgggcggctt gggcagcatt caggcgcagc
     9781 agacgcataa agacgaaatc cccgaaggcg cgttgctgat ccaactgggc ggcccgggta
     9841 tgcttatcgg cttgggcggc ggcgcggctt cttcgatgga taccggtaca aacgacgctt
     9901 ctttagactt caactccgtg caacgcggca accccgaaat cgaacgccgc gcgcaggaag
     9961 tcatcgaccg ctgctggcag ctcggcgaca aaaacccgat tatctccatc cacgacgtgg
    10021 gcgcaggagg tttgtccaac gccttccccg aactcgtcaa cgatgccgga cgcggcgcgg
    10081 tattcaagtt gcgcgaagtg ccgttggaag aacacggttt aacgcctttg caaatctggt
    10141 gcaacgaatc gcaagagcgt tatgtgttgt cgattttgga aaaagatttg gatgccttcc
    10201 gcgccatctg cgaacgcgaa cgctgcccgt ttgccgtagt cggcacggcg accggcgacg
    10261 gccatttgaa agtacgcgac gatttgttct ccaacaaccc tgtcgatttg ccgttgaacg
    10321 ttttgctcgg caaaccgccc aaaaccacgc gcaccgacaa aacggttacg ccgtatgcga
    10381 ccgaagggaa tccctttggg aaaaaaccgt ttaatgccgg cgatatcgac attaccgaag
    10441 ccgcctaccg cgttctgcgc ctgcctgccg tagcggctaa aaacttcctg attaccatcg
    10501 gcgaccgcag cgtcggcggc atgacccacc gcgaccaaat ggtcggcaaa taccaaaccc
    10561 ccgtagccga ctgcgccgtg accatgatgg gcttcaacac ctatcgcggc gaagcgatgt
    10621 ctatgggcga aaaaccggcc gtcgccctgt ttgacgcgcc tgcttcgggc agaatgtgta
    10681 tcggcgaagc catcaccaac atcgcagcgg tcaacatcgg cgacatcggc aatatcaaac
    10741 tttctgccaa ctggatggcg gcgtgcggca acgaaggcga agacgaaaaa ctctaccgca
    10801 ccgtcgaagc ggtttccaaa gcctgtcagg cattggattt gagcatcccc gtgggcaaag
    10861 acagcctgtc gatgaaaacc gtttggcagg atggcgcgga caagaaatcc gtcgtctcgc
    10921 cattgagcct gattatctcc gcgttcgcgc cggtggaaga cgtacgcaaa accgttacgc
    10981 ccgaactgaa aaacgtcgaa gacagcgtat tgctgtttat cgatttgggc ttcggcaaag
    11041 cgcgtatggg cggctcggcg tttggtcagg tgtacaacaa tatgagcggc gacgcgcccg
    11101 atttggacga tacaagtcgt ctgaaagcgt tttacaacgt gattcagcag cttgtcgccg
    11161 aagacaaact cttggcgtat cacgaccgca gcgacggcgg tttgtttgcc gttttggtag
    11221 aaatggcgtt tgcggggcgg tgcggcttgg atatagattt aaatttattg cttgcacaaa
    11281 catttattac caaccatacc gctctgtctc aatcattgcg gactgaagag gtaaaagcgt
    11341 tggctgaatg gcaagaaacc attgcccgca cattatttaa tgaagagttg ggtgctgtta
    11401 tccaagttag aaaacaagat gttgccgata ttatcaattt attctatcaa caacagctgc
    11461 atcataatgt ctttgaaatc ggtacgttaa ctgatgagaa cacgttaatc atccgcgacg
    11521 ggcaaacgca ccttatttct gacaacctaa tcaaactgca acaaacctgg caagaaacca
    11581 gccatcaaat ccaacgcctg cgcgacaacc ctgcctgcgc cgacagcgag tttgccctga
    11641 ttggcgacaa cgaacgcagc gcattgtttg ccgacgtgaa gttcgacgtg aacgaagaca
    11701 tcgccgcgcc gtttatcaac agcggcgcga aacccaaaat cgccatcctg cgcgaacagg
    11761 gcgtaaacgg gcaaatcgaa atggccgccg cgttcactcg cgccggtttc gatgcctacg
    11821 acgtgcatat gtccgacctg atggcaggcc gcttccgcct tgccgacttc aaaatgctgg
    11881 cggcgtgcgg cggcttcagc tacggcgacg tactcggcgc gggcgaaggc tgggcgaaat
    11941 ccatcctgtt ccaccctgcc ttgcgcgacc aatttgccgc cttctttgcc gatccggaca
    12001 cgctgacatt gggcgtgtgc aacggctgcc agatggtcag caacctcgcc gagattatcc
    12061 cgggtgcaga ggcatggcct aaattcaaac gcaacctgag cgaacagttt gaagcacgcc
    12121 tgagcatggt tcacgttccg aaatcagcgt cgctgattct gaacgaaatg caaggctcca
    12181 gcctgcctgt cgtggtcagc cacggcgaag gccgcgccga cttcgcgctt cacggcggca
    12241 atatttccgc cgatttgggc atcgcgctgc aatatgtgga cggtcaaaac caggttaccc
    12301 aaacctatcc gctcaacccc aacggctcgc cgcaaggcat cgccggcatt accaacgccg
    12361 acggccgcgt gaccatcatg atgccccacc ccgaacgcgt gtaccgcgcc gcgcaaatga
    12421 gctggaaacc ggaaggctgg acggaactgt ccggctggta ccgcctcttt gccggcgcac
    12481 gtaaagcctt gggctaaccg ccctactcaa accaatgccg tctgaagaat atttcagacg
    12541 gcgttccggc ataccatcct ttaaacggta tccgtccacc gaggaacact catgaaaatc
    12601 acccccgtca aagccctaac cgacaactac atctggatga tacagcacgg caaccatgcc
    12661 gtctgcgtcg acccttccga accctcgccc gtcttagaat tcctcgtccg caaccgcctc
    12721 atgcttgccc aaacatgggt aactcacccc caccccgacc acgagggcgg tgcggcggca
    12781 ctctggcgcg gctacatgga atcgcccgtt tacggcgaat ccgacatcga agcagcaacc
    12841 cacaccgtaa ccgccggcac ccaattcacc ttcggcgacg gacaggttac cgtttgggca
    12901 acacccggcc acacagaccg ccacaccagc taccttctcg aaacttcaga cggcatacac
    12961 gtcttttgcg gcgacaccct tttttccgcc ggctgcggac gcgtgtttac cggcacaatc
    13021 gaacagcttt acgacagctt ccaacgcttc aaccgcctgc ctgaaaacac cctgttctac
    13081 ccggcacacg aatacaccgc cgccaacctg cgtttcgccg cccatatcga gccggacaac
    13141 gccgacattc agacggcact gaaagcggcg gcgcatacgc ctaccctgcc cgttaccctc
    13201 gcgcacgaac gccgcgtcaa tccgtttttg cgcgtcgacc tgccgcacgt cagagaccgc
    13261 gccgaggcat tgagcgggaa aacgttaaac agcagcctcg atacctttgt cgcgctgcgt
    13321 gaacttaaaa accaataccg gacgaaataa aacaatggga aaacgcagcc attcctagga
    13381 tttttattaa aatcttaaat aaaatcatac aatcatcgcc aatagacgaa aggacaccgt
    13441 tgccttataa tcaaacaaaa acaaaatata taatatagtg gattgaattt aaatcaggac
    13501 aaggcgacga agccgcagat agtacggcaa ggcgaggcaa cgctgtactg gtttttgtta
    13561 atccactata ttgttaatcc actatataaa tccagcacaa aacgggatcg gtgattcttg
    13621 tccgcaagaa tcgttgattt tctctattac acggataatc atcatgcgct tcacacacac
    13681 caccccattt tgttccgtat tgtccaccct cggtcttttt gccgtttccc ctgcttactc
    13741 atccattgtc cgcaacgatg tcgattacca atattttcgc gactttgccg aaaataaagg
    13801 cgcgttcgcc gtaggtgcaa gcaatatttc catccaagac aagcaaggca aaatattagg
    13861 cagggttctc aacggcatcc ccatgcccga cttccgagtc agcaaccggc aaaccgccat
    13921 cgccaccctg attcaccccc aatacgtcaa cagtgtcaaa cacaacgtcg gctacggttc
    13981 catacaattc ggcaacgaca cccaaaatct agaagaacaa gcctatacct accgcctcgt
    14041 atcacgcaac ccgcacccgg actacgacta ccaccttccc cgcctcaaca aactggttac
    14101 cgaaatctca cctgccgcac tcagcagcgt acccttgctt ggaaacggcc agccaaaggc
    14161 caatgcctac ctcgataccg accgcttccc atactttgta cgactcggct caggcacgca
    14221 acaagtccgc aaagcagacg gcacgcgtac acgaaccgcc ccggcatacc aatacctgac
    14281 cggcggcacg ccgctgaaag tattggggtt ccaaaaccac ggcttactcg tcggcggcag
    14341 cctgaccgac caacccctta acacctacgc aatcgccgga gacagcggtt cccccctgtt
    14401 tgccttcgac aagcatgaaa accgctgggt gcttgcgggc gtactcagca cctacgccgg
    14461 cttcgataac ttcttcaaca aatacatcgt cacgcaaccc gaattcatcc gttccaccat
    14521 ccgccaatac gaaacccggc tggatgtcgg gctgaccacc aacgaactca tatggcgcga
    14581 caacggtaat ggcgacagca ccctgcaagg gctcaacgaa cgcatcaccc tgcccattgc
    14641 aaacccttcg cttgccccac aaaacgacag caggcacatg ccgtctgaag atgccggcaa
    14701 aacgctcatc ctatccagca ggttcgacaa caaaacactg atgctggcag acaatatcaa
    14761 ccaaggcgca ggcgcattgc agttcgacag caacttcacc gtcgtcggta aaaaccacac
    14821 atggcaaggt gcaggcgtta tcgtagccga cggcaaacgc gtcttctggc aagtcagcaa
    14881 ccccaaaggc gaccggctct ccaaactggg cgcaggcacg cttatcgcca acggacaagg
    14941 catcaaccag ggcgacatca gcatcgggga aggcactgtc gtactcgccc aaaaagctgc
    15001 ttcagacggc agcaaacaag cattcaacca agtcggcatc accagcggca ggggcacggc
    15061 cgtcctcgcc gacagccagc aaatcaaacc cgaaaacctc tatttcggct tcaggggcgg
    15121 acggctcgac ctcaacggca acaaccttgc ctttacccat atccgccatg cggacggcgg
    15181 cgcgcaaatc gtcaatcaca accctgacca agccgcgaca ctgacgctga ccggcaaccc
    15241 cgtcctcagt cccgagcatg tcgagtgggt gcaatggggc aaccgtccgc aaggcaacgc
    15301 ggcggtttac gaatacatca acccgcaccg caaccgtcgg accgactact tcatactcaa
    15361 acccggcggc aacccgcgcg aatttttccc gttaaatatg gaaaactcaa caagctggca
    15421 atttatcggc aacaacaggc aacaggccgc caaacaagtc gcccaagccg aaaatgcccg
    15481 ccccgaccta attaccttcg gcggatactt gggtgagaac gcgcaaacgg gcaaagccgc
    15541 accgagttac agcaaaacca atatcgaatg ggtgaatggt ccaaacaccc cacacaagcc
    15601 ctcgccctat acgaatacgc caacccgcac aggggcaggc gtaccgacga ttaccgcctc
    15661 aaagaaggcg gcaaccccgg cgcctacttc ccatggaatg cctacagcac ccaagactgg
    15721 gaatttctcg gacatgaccg gcgaagcagc catagaaaaa acccgccata tcgcaaatgc
    15781 cgccgtatac ggccggcccg aataccgtta caacggcgca ctcaacctgc actatcgtcc
    15841 caaacgcacc gacagcacgc tgttgctcaa cggcggcatg aaccttaacg gggaagtctt
    15901 gattgagggc ggcaatatga ttgtgtcagg caggcccgta ccccatgcct acgaccacca
    15961 ggccaaacgc gaacccgttc ttgaaaacga atggaccgac ggcagcttca aggctgcacg
    16021 gttcaccctg cgaaaccatg cccgactgac ggcagggcgc aataccgcgc atctggacgg
    16081 cgacataacc gcatacgatc tgtccggcat cgacctcggc tttatccaag gcaaaacacc
    16141 ggaatgctac cgctcctacc atagcggcag cacccactgc acacccaacg ccgttttaaa
    16201 agccgaaaac tatcgtgcac tacctgcaac gcaagtacgc ggcgacatta cccttaacga
    16261 ccgttcagag ctccgcctgg gcaaagcaca cctgtacggc agcatccgtg ccggcaaaga
    16321 caccgcagtc cgcatggaag cagacagcaa ctggacactt tcccagtcca gccacaccgg
    16381 cgcactgacg cttgacggcg cacaaattac cctgaacccc gatttcgcca ataatacaca
    16441 caacaaccgc ttcaacacac tgaccgtcaa cggcacactt gacgggttcg gcacattccg
    16501 attcctgacc ggcatcgtcc gaaaacaaaa tgcccccctc aaactggaag gggacagccg
    16561 cggcgcattc caaatccacg tcaaaaacac cggacaagaa cctcaaacaa ccgaatcgct
    16621 tgcacttgtg agcctcaatc cgaaacacag ccaccaagcc cgattcaccc tccaaaacgg
    16681 ctatgccgat ttgggtgcct accgctacat cctccgcaaa aacaacaacg gatacagcct
    16741 gtacaacccg ctcaaagagg ccgaacttca aattgaagcc acgcgtgcgg aacatgagcg
    16801 caaccaacag gcatacaacc aattacaggc aaccgacatc agcagacagg ttcaacatga
    16861 ctctgacgcg accaggcagg cactacaggc ctggcagaac agtcaaaccg aacttgcccg
    16921 catcgacagc caagtccaat atctgtccgc ccaattgaaa cagacagacc cgctgaccgg
    16981 cattctgacg cgtgcccaaa acctgtgtgc cgcacaagga tacagtgtcg atatctgccg
    17041 tcaggttgcc aaagccgccg acacgaacga cctgacactc ttcgaaaccg aactggatac
    17101 gtatatagaa cgtgtagaaa tggccgaatc cgaacttgac aaagcacggc aaggcggcga
    17161 tgcgcaagcc gtcgaaacag cccggcacgc ctacctgaac gcactcaacc gtctgtcccg
    17221 acaaatccac agtttgaaaa ccggcgttgc cggcatccgt atgccgaacc tggccgaact
    17281 gatcagccgg tcggccaaca ccgccgtttc cgaacaggcc gcctacaata ccggccggca
    17341 acaggcggga cgccgcatcg accgccacct taccgatccg cagcagcaaa acatctggct
    17401 ggaaaccggt acgcaacaaa ccgactacca tagcggcaca caccgtccct accaacaaac
    17461 taccaactat gcacatatca gcatccaaac cggcatcacc gaccgtctca gtgtcggtac
    17521 aattttaacc gatgagcgca caaacaaccg ttttgatgaa ggcgtatccg cccgaaaccg
    17581 cagcaacggc gcacatctgt tcgtcaaagg ggaaaacggc gcactctttg ccgcggcaga
    17641 tttaggctac agcaacagcc gtacccgatt taccgattat gacggggctg ccgtccgccg
    17701 ccacgcatgg gatgcaggca tcaacaccgg catcaaaatc gataccggca tcaacctcag
    17761 accctatgcc ggcatccgta taaaccgcag caacggcaac cggtacgtac tcgacggcgc
    17821 agagataaac agcccggcgc aaatccaaac cacatggcat gccggcatcc gtctcgataa
    17881 aaccgtcgaa ctgggtcaag ccaagctgac ccccgccttc agcagcgatt actacgatac
    17941 ccgccaaaac agcggttccg ccctcagcgt caacgaccgt accttactgc agcaagccgc
    18001 ccacggcaca ctgcataccc tgcaaatcga cgccggatac aaaggctgga acgccaaact
    18061 tcatgccgct tacggcaaag acagcaacac cgcccgccac aaacaagcag gaatcaaaat
    18121 aggctacaac tggtaacaag ccgataaaaa tgccgtctgg aacccgcgtt tcagacggca
    18181 tttgcgttaa aaatagtaaa ccgttccaaa agggagtaga atagtgccgt ttccaaccct
    18241 gcgcctgtac cgtcaggctt ttattatgga ccttcccagt tcgtttttac tgaacgcccc
    18301 ttccgattcc aaacgacaat aaccatcccg ccggaatgcc tccccgcaca cggcgggcgg
    18361 agcatttatg agcatcgaac caacccctcc gaaccttgaa aacgacggta tcgaaaacga
    18421 tgtagaacgc gtttccgccg atttcgaccg tgtccactcc ctctgcgaaa ttctcgaacc
    18481 tgcttttgaa caaatcgaaa acggcacacc gcttgaagac gcgccgctgc gcgacaagct
    18541 gaccgagctg accgttctct tgagcgagct gcaccctgcc gacgtggcgg cggtattgga
    18601 atcgctaccg ccgcgcgaac gcaatatcgt ctggattctg gtcaaaccgg aagacgacgg
    18661 cgaagtattg ctggaagtat ccgacgcggt gcgcgaaacg ctgatcgagt cgatggacaa
    18721 agacgaattg ttggcagcgg tcgatgattt ggacgcggac gaattggcgg aactggcaga
    18781 cgatttgccg caccaagtgg tttacgaagc gctacaaacc cgcgatgagg aagaacgcgc
    18841 ccaagtcaaa gcggcaatgt cctacgaaga caaccaagtc ggtgcgatta tggacttcga
    18901 gttggtcagc atccgcgccg atgtcgcctg tgaagtggtg ctgcgctatc tgcgccgctt
    18961 cgacagcctg cccgaccata ccgacaagat ttttgtggtc gatgaaaacg acgtactgca
    19021 aggcgtgctg cccatccgca aacttttggt cgccgatccc gaagatttgg tggaaaacgt
    19081 gatggcgaaa gatgtcgtgc gtttccgcgc cgaagacgac gtggaagaag cggcgcaggc
    19141 gtttgaacgc tacgacttgg taactgcgcc ggttgtcgat gaaaacaaaa agctcatcgg
    19201 taggattacc gttgacgaga tggtggacgt aatccgtgaa gaatccgaag cggatatgtt
    19261 gaacatggca ggtttgcagg aagaggaaga cctgttcgcc cccgtcttgg attccgtcaa
    19321 aaaccgctgg atgtggctcg ccgtcaacct ctgcaccgcc ttcctcgcca gccgcgtcat
    19381 cggcgcgttt gaaggcagta tcgaaaaaat cgttgcactc gccgcgctga tgcccatcgt
    19441 cgccggtatc ggcggcaact cgggcaacca gacgattacc atgattgtcc gcgccatggc
    19501 gatggggcag ttgacgggaa tgcaggcggg gcgcttgttg aaaaaagaag tcggcgttgc
    19561 cctagtcaac ggcattatct ggggaaccgt catgggcgcg gtttcatggc tgctctacgg
    19621 cagcctcggc atcgggctgg ttatgattgc cgcgatgacg ctcaacctcc tgctggcggc
    19681 aaccgtcggc gtattaattc ccgtggtaat ggaaaagttc ggacgcgatc ccgcactggg
    19741 cagctcggtg ctgattaccg ccgttaccga ctccggcggc ttcctgattt tcttggggct
    19801 cgccacccta ttcctgcttt aaatgccgtc tgaacccgcg caaaaatgcc gtctgaagcg
    19861 gaagctgctt cagacggcat ttgactattt atccttgttg cacaagatta ttggacggta
    19921 tgccggggca gccctttggc aacgccgacc acatcctccc cgaacagcgc gttgacatcg
    19981 gtttcgtcaa acacatattt gctgtggcag aaatcgcaat cgacttcgat gctgccttgt
    20041 tccgccacca cgccgccgac ttcttccccg cccagcatca acagcatatc gctgactttg
    20101 ccgcgcgaac aggtgcatga aaattcaaac gtttccggct cgaacacgcg cggcggcgtt
    20161 tcgtggaaca agcggtacaa aacatgttgc gcatccaatt ctgccaactc gcccgccgtc
    20221 agcgtgcgcg ccagcgtact gacgtgttcc catgcctctt catccaacac ctcttcaggc
    20281 agacgctgca ccagcagacc gcccgccgct tcatcgcttg cagacaggac gatgtgcgta
    20341 tcaagctgtt cggaacgttt catatagttc atcaacattt gcgcgatacc gtcgccttcc
    20401 aaaggcacta cgccctgcca gggttcgccg tctttgggct gcagcgtcaa tacgaacacg
    20461 ccgttgccgc ccaaaaggtc gccgaggctt tcgtcatcgg ctatttctgc cgcctcgtcc
    20521 caccgcgcgg tcgcacggac ggtacggtcg gaagtcgctt ccgcaaccag cattttcagc
    20581 cgcccctgcc cctgaacctg cacaatcagc gtgccttcgt ttttgaggtt gcccgacagc
    20641 agaacaccgg cagccaacag ctcgcccaag gcgcggcgga tggcggcggg atagtttttc
    20701 tgttttacaa tgtgctgcca cacgttttcc agacggacgt gcagcccgcg cacgggcata
    20761 tcgtcgaaga taaagcgggt acgcacatcg gcgcggttga tggcggtttg attcatgatt
    20821 ttctctgact gattgttcgg atggcggcta tatggttgcg gtcggcgcga aaacaagacg
    20881 gacggcggat gcgcttccca aattatcaat aaattatata aaaatcaaca tattaactca
    20941 atctaacaag ccgttttttg ccaaacagcc gtttttttat atacaatcaa caagatattt
    21001 tcgactgata cagcataaca tcgcacggcg gcacgatgcc tcctgcgcgg aaacaccgat
    21061 atggattctt ttttcaaacc ggcagtttgg gcggttttgt ggctgatgtt tgccgtccgc
    21121 cccgcccttg ccgacgagtt gaccaacctg ctcagcagcc gcgagcagat tctcagacag
    21181 tttgccgaag acgaacagcc cgttttaccc atcaaccgag cccccgcccg gcgggcgggc
    21241 aatgccgacg aactcatcgg cagcgcgatg gggcttaacg aacagcccgt tttacccgtc
    21301 aaccgagtcc ccgcccggcg ggcgggcaat gccgacgaac tcatcggcaa cgcgatgggg
    21361 cttaacgaac agcccgtttt acccgtcaac cgagcccccg cccggcgggc gggcaatgcc
    21421 gacgaactca tcggcaacgc gatgggactt ttgggtattg cctaccgcta cggcggcaca
    21481 tcggtttcta ccggttttga ctgcagcggc ttcatgcagc acatcttcaa acgcgccatg
    21541 ggcatcaacc tgccgcgcac gtcggcagaa caggcacgga tgggtacgcc ggttgcccga
    21601 agcgaattgc agcccggaga tatggtgttt ttccgcacgc tcggcggcag ccgcatttcc
    21661 catgtcggac tttatatcgg caacaaccgc ttcatccacg cgccgcgcac ggggaaaaat
    21721 atcgaaatca ccagcctgag ccacaaatat tggagcggca aatacgcgtt cgcccgccgg
    21781 gtcaagaaaa acgacccgtc ccgctttctg aactgatttc ccaaggaata cgcaatgagt
    21841 atgcccgaaa tacccaaatg gtacgacgat gacggacaga tcgtgtcctg taccgaaaag
    21901 atcaaagtga tgtccgaaaa tatggccgag ctgtatcaga cggcacaaga cgcgtttgaa
    21961 gacgcgctgc tgatgggttg cggcgaacgt cagttgcgcg attacctgct cgcgctgatt
    22021 gaaggtttgg aaaatcccta ccgcaaagtc tgaacacgcc ccggttgctg cggcacggtt
    22081 tatccgtgcc gtttttgcgt ttgtgcgcgg cttcggcttt tcagacggca tatttgacgt
    22141 tatgattaaa cagttaacaa gatttatcac aacgccgtca aaagacagac acacaacatg
    22201 aacatcatcc gcgcgctcct catcatcctc ggctgcctcg ccgccggcga aaccgccgtt
    22261 ttcctagcag gcatcaaact gcccggcagc atcgtcggca tgggcgtgct gtttgcgctt
    22321 ttgcaggcgg gttgggtcaa aacgtcttgg ctgcaacagc ttaccgacgc gctgatggcg
    22381 aatctgacgc tgtttctcgt gccgccctgc gtggcggtca tcagctattt ggatttgatt
    22441 gccgacgatt ggttttcgat actggtttcc gcctccgcca gcactttgtg cgtactgctg
    22501 gttacaggca aggttcaccg ctggatacgg agcattatct gatgaacgaa atcctcaggc
    22561 agcccagcgt tctgcttttc ctcacgcttg ccgtgtacgc gcttgcgatt atcgtgcgca
    22621 cgcgcacggg caatatcttc tgcaaccccg tactcgtcag cactatcgtg ctgattgcct
    22681 acctgaaaat cctcgacatc gattatgcgg tgtaccacaa cgccgcgcag tttatcgatt
    22741 tctggctcaa gcccgccgtc gtcgtgcttg ccgtgccgct ctaccaaaac cgccgtaaaa
    22801 tcttcaacca atggctgccc gtcatcgttt cacagcttgc gggcagcgtt acgggcattg
    22861 ttacggggat gtattttgcc aaatggctgg gcgcggaacg cgaagtcgtc ctctcgctcg
    22921 cgtccaaatc tgttaccaac cccatcgcca ttgaaattac ccgctccatc ggcggcattc
    22981 ccgccattac cgccgccacc gtcatcattg ccggtctggt cggacagatt gccggttaca
    23041 aaatgctgaa gaacacagtc gtcatgccct cgtccgtggg tatgtcgctc ggcacggctt
    23101 cgcacgcgat gggcattgcc gcctcgctcg aacgcagccg ccgtatggcg gcatacgcgg
    23161 ggctggggct gacgttcaac ggcgtactga ccgcgctgat tgcgccgctg ctcatccccg
    23221 ttttgggatt ttgaacccgt ttcagacggc atttcagccc atgctgtctg aacgccgaca
    23281 cactcgcaag gagaaccgtt atggctgtca acctgaccga aaaaaccgcc gaacaactgc
    23341 ccgacatcga cggcattgcc ctctacaccg cccaagcagg cgtgaagaag cccgggcata
    23401 ccgacctgac actgattgcc gtagccgccg gcagcaccgt cggtgcagtc ttcacgacca
    23461 accgtttctg tgccgcgccc gtccacatcg ccaaatcgca ccttttcaac gaagacggcg
    23521 tgcgcgccct catcatcaac acgggcaacg ccaacgcggg tacgggcgca cagggcagaa
    23581 tcgatgcttt ggcagtgtgt gccgccgccg cccggcaaat cggctgcaaa ccgaaccagg
    23641 tgctgccctt ctccaccggc gtgattctcg aaccgctgcc cgcagacaaa atcatcgccg
    23701 ccctgcccaa aatgcagcct gccttctgga acgaagcggc acgcgccatc atgaccaccg
    23761 acaccgtgcc caaagccgcc tcgcgcgaag gcaaggtcgg cgacaaacac accgtccgcg
    23821 ccacgggcat cgccaaaggc tcgggcatga ttcatcccaa tatggcgacc atgctcagtt
    23881 tcatcgccac cgatgccaaa gtttcccaac ccgtcctcca actgatgacg caggaaatcg
    23941 ccgacgaaac cttcaacacc atcaccgttg acggcgacac cagcaccaac gacagcttcg
    24001 tcatcatcgc caccggcaaa aacagccaaa gcgaaatcga caacatcgcc gacccgcgtt
    24061 acgcccaact caaagaattg ttgtgcagcc tcgcgctcga actcgcccaa gccatcgtcc
    24121 gcgacggcga aggtgcgacc aagttcatca ccgtccgcgt cgaaaacgcc aaaacccgcg
    24181 acgaagcccg ccaagccgcc tacgccgtgg cacgttcgcc gctggtcaaa accgcctttt
    24241 tcgcctccga ccccaacctc ggcaggctgc tcgccgccat cggttatgcc ggcgttgtcg
    24301 acctcgatac tgacctcgtg gaaatgtatc tcgacgatat tttggttgcc gaacacggtg
    24361 gacgcgccgc aagctacacc gaagcacaag ggcaggcggt gatgtcgaag gccgaaatca
    24421 ccgtccgcat caagctgcat cgcggacaaa gtgccgccac cgtctatacc tgcgacctgt
    24481 cgcacggata cgtttccatc aacgccgact accgttcctg acccgacacg gcttcagacg
    24541 gcatacataa aatgccgtct gaaccgccgg acaacatgcc atgacctcca cattcccccg
    24601 ccgcctcgcc cgcaaaatcc gccaaacccg ccgcctgtcg cgcaaaagca tcgcctttct
    24661 gttccttttg gcaggttcgg cactcgtcgc cctgaccgcg ctgttttttg cccatcttgc
    24721 cgattttgcg ctggaactga acgccaaact ggttcaacaa tacccgtggt tcgcgtgggt
    24781 cgcgcttcct ttgggtttgc cgcttattgc gtggctcaca cgcaaattcg cccccttcac
    24841 cgccggcagc ggcatcccgc aggtcatcgc ctcactgtcg ctgccctacg gcgcacagaa
    24901 aacgcggctg atccgcctcg ggcagacgct gctgaagatt ccgctgacct ttttgggtat
    24961 gctgttcggc gtgtccatcg gacgcgaagg tccgtccgtg caggtcggcg cggcagtgat
    25021 gggcgcgtgg ggcgcgtggt gcaagaaaca cggcttggca ttcaaaggga tgcaggaaaa
    25081 cgatttgatg gcggcgggcg cggcgggcgg tttggcagcc gcgttcaacg cgccgctggc
    25141 gggcgtgatt ttcgccattg aggaactcgg gcgcggcatc atgttgcgct gggagaggca
    25201 aattcttttg ggcgtgctca cctccggttt catacaggtc gccattcagg gcaacaaccc
    25261 gtatttttcc ggcttcaacg gcggcgtatt ggaacatatc tttctgtggg tcgcactgtc
    25321 cggcctggtt tgcggcgcgg cgggcgggct gttcggacgt ttgctctatc gcggtgcggc
    25381 ggcatttgca ccacgcaaga tacgcggctt catccgcaac cgtccgctgc tgctggcggc
    25441 actgatgggg ctgctgctcg ccctgctcgg cacgttctac caaggcaaaa cctacggcac
    25501 cggctaccac gaagccgccc aagccctgca cggcatctat gaagccccct tcggactcgc
    25561 cgccgccaaa tggctcgcca ccgtattcag ctattgggca ggcgttccgg gcggcatttt
    25621 cactccctcg ctgaccatag gcgcggtttt gggcgagcat atcgccgcca tcgccgacat
    25681 atcgcagggt gcaaacatca tcgtcctcat ctgcatggcg gcatttctgg cgggcgcgac
    25741 acaatccccg attacttccg ccgtcgtcgt catggaaatg acgggcggac aaagcctgct
    25801 gttttggatg ctaattgcct gcattttcgc ctcgcaggtt tcgcgccagt tttcgccgcg
    25861 tccgttctac cacgcatcgg gaatgcgctt ccgccagcgc gtgcttcagg aaaccaccgc
    25921 ccaaaccggc aatgcgcccg caagaccgca aacagcaaac agcaaaacgg gaatgccgtc
    25981 tgaaaattaa aacgcccccg atcaaacgcc ggcagccgcc ttgatttgaa taccgttccg
    26041 ccgccgcttg aaatttcagc aacaatgccg tctgaacgac agaatgcggt tttcagacgg
    26101 catttccaca tcccgatatt gcctaaacaa aaccgaagcg tttgctataa ttctattttt
    26161 taccgcacac gcaccaatca tgtttcccga tttctcccaa accctctcca aagaccgcca
    26221 cttcctacgt tccgccttca aaaatcccaa caaatacggc gggttgtcca aagtcgaaga
    26281 aaaataccga aaatcgcacg aaatcttttt gaaacgtttg gcagccttgc ccaaacccga
    26341 attcgacaac accctgcccg ttcacgaaaa acttgacgaa atcaaaaaaa ccgttgccga
    26401 acatcaggta acgattattt gcggcgaaac cggttcgggc aagaccactc agttgcccaa
    26461 gatttgtctg gaactcgggc gcggggcggc gggtttgatc gggcataccc agccgcgccg
    26521 tttggcggcg cgttccgtag cagagcggat tgccgaagag ctgaaatccg aaatcggcag
    26581 cgcggtcggc tataaagtgc gcttcaccga ccacacctcg cgcgatgcct gcgtcaagct
    26641 gatgaccgac ggcatcctgc tggcggaaac ccagaccgac cgttatctcg ccgcctacga
    26701 cacgattatc atcgacgaag cgcacgagcg cagcctgaac atcgacttcc ttttgggcta
    26761 tttgaaacaa ctcctgccgc gccgccccga tttgaaagtc atcatcacct cggcaacgat
    26821 agacgcagaa cgcttttccc aacacttcaa cggcgcgccc gtgctggaag tgagcggacg
    26881 cacctatccc gtcgaaatcc tctaccgacc gctgatcggc aaagacgaag acgacgcgga
    26941 aatagaattg accgacgcga ttgtcgatgc ggcggacgaa ttggcgcgat atggcgaagg
    27001 cgatattttg gtattcctgc cgggcgaacg cgaaatccgc gaagccgccg aagccctgcg
    27061 caaatccacg ctacgccgca acgacgaaat cctgcccctg ttcgcacgcc tgtcgcacgc
    27121 cgagcagcac aaaatctttc acccctccgg agcgaaacgc cgcatcgtgc tggcaaccaa
    27181 cgtcgccgaa acctcgctta ccgtgccggg catcaaatac gtcatcgaca ccggcctcgc
    27241 gcgcgtcaaa cgctattccg cacgggcgaa agtggagcag cttcatatcg aaaaaatctc
    27301 ccaagccgcc gcccgccaac gctccggccg ctgcggacgc gtctccgcag gcgtatgtat
    27361 ccgattgttt tcagaagaaa atttcaacaa ccgccccgaa tttaccgacc ccgaaatcgc
    27421 ccgcagcaac ctcgccgccg tcatcctgcg catggcatcg ctgaaactcg gcgacgtagc
    27481 ggcattccca tttttagaaa tgcccgattc gaggtatatt aatgatgggt ttcaggtgtt
    27541 gctggaatta ggggcagtgg aggccgcctg aaaacaagta ggatatacac ttctacctga
    27601 caaagaccaa caaacgataa aactacggca catctagatg cccaacctac aacattgcac
    27661 acggtaaaaa catgaaacac ttactcatcg attttgaaaa cgtccagccg caaaacttag
    27721 acaaattact aaccgaaaat acccatattt ggctatttat aggtgtatta cacaaaatgt
    27781 tacctattag tctggtgcaa tccctactac gtttcggcga acgtgtccat cttgtccagt
    27841 tacaaaaaac ggggaaaaac gcattggatt tttacctgtc ctattacctc ggacaaatta
    27901 ccgccacaga ccccaatgcc caaatcggca tactctcgcg tgatggagga tacgatgttc
    27961 tggtcgaaca tattttgaaa aaccaccagg cgaagggtat cgtgcgccta gccaatatag
    28021 atgaagtaca acatcagaaa attactaccg aaccgccgtc agcattgctg gaaaacactc
    28081 ctcagcctga aaccaccctc aaaccacagc aaccattaac ttcctatttc caagcagccc
    28141 taactgcact gcgccgcccc gacgctttcc gcccctgccg cctgcataac ctgcgacaaa
    28201 atctgcgtaa gcatattttg agtgatttgt ttaaagaaaa aaccgatgaa gaatgcgaaa
    28261 taaccactgc taacgttatc aataaactca aagcacaaaa cttcatcagc attgatgaac
    28321 aggaaaccgt ttcctaccat ctcagtgata atgatttgtt acaaagaatc caacgccata
    28381 ttttaagcca acgtcccaaa acctacgctg attttcaagc cgtcgtgcaa aaccgagcag
    28441 atgcacttca cttaacagtc ggtaccaacg acattcaatc ctttgcgcga catttgcgcg
    28501 accaaaacct gatccgccaa aacaatggga aaattgaata tgcaccgttt actgaaccta
    28561 aaccacagcc aacgcccaag cagcctaaaa aaaccgcatg ggaacctgat gaaattattt
    28621 ggaaaaaagt gattgccgcg ttatcgttaa agaaccgtcc taataaaacc aaaactttac
    28681 gcaatacaat ccaggcactc acaaaatcca atgcacaaga aactgacaaa ctgctacaac
    28741 atttacaaga tacccaagtc ctacgtattg acggtactaa aattgtgtat atcaagtagg
    28801 atgggcattt atgcctgacc tacaaaacca aaaacaacct aacaccctta atcaactcca
    28861 caaacccttt tcagacaacc tcgttttttg aaaaacaatc tgtaaacaga taactgctga
    28921 agaataccgt tgccgagccc caaaacctgc ctgcaacttt tattgtgaac tgcccattat
    28981 gaaaaaatcc cttttcgttc tctttctcta ttcatctcta cttaccgcca gcgaaatcgc
    29041 ctatcgcttt gtattcggaa ttgaaacctt accggctgca aaaatggcag aaacgtttgc
    29101 gctgacattt gtgattgctg cgctgtatct gtttgcgcgt tataaaactt cgcggctgct
    29161 gattgcgctg tttttcgcgt tcagcattat tgccaacaat gtacattatg cggtttatca
    29221 aagttggatg acgggtatta actattggct gatgctgaaa gaggttaccg aagtcggcag
    29281 tgcgggcgcg tcgatgttgg ataagttgtg gctgcctgcg ttgtggggcg tggcggaagt
    29341 cgtgctgttt tgtagccttg ccaagttccg ccataagacg catttttctg ccgatatatt
    29401 gtttgccttc ctaatgctga tgattttcgt gcgttcgttc gacacgaaac aagagcacgg
    29461 tatttcgccc aaaccgacat acagccgcat caaagccaat tatttcagct tcggttattt
    29521 tgtcggacgc gtgttgccgt atcagttgtt tgatttaagc aagattcctg tgttcaaaca
    29581 gcctgctcca agcagaatcg ggcaaggcag tattcaaaat atcgtcctga ttatgggcga
    29641 aagcgaaagc gcggcgcatt tgaaattgtt tggctacggg cgcgaaactt cgccgttttt
    29701 aacccggctt tcgcaagccg attttaagcc gattgtgaaa caaagttatt ccgcaggctt
    29761 tatgacggca gtatccctgc ccagtttctt taacgtcata ccgcatgcca acggcttgga
    29821 acaaatcagc ggcggcgata ccaatatgtt ccgcctcgcc aaagagcagg gctatgaaac
    29881 gtatttttac agcgctcagg ctgaaaacca aatggcaatt ttgaacttaa tcggtaagaa
    29941 atggatagac catctgattc agccgacgca gcttggctac ggcaacggcg acaatatgcc
    30001 cgatgagaag ctgctgccgc tgttcgacaa aatcaatttg cagcagggca ggcattttat
    30061 cgtgttgcac caacgcggtt cgcacgcccc atacggcgca ttgttgcagc ctcaagataa
    30121 agtattcggc gaagccgata ttgtggataa gtacgacaac accatccaca aaaccgacca
    30181 aatgattcaa accgtattcg agcagctgca aaagcagcct gacggcaact ggctgtttgc
    30241 ctatacctcc gatcatggcc agtatgttcg ccaagatatc tacaatcaag gcacggtgca
    30301 gcccgacagc tatctcgtgc cgctagtgtt gtacagcccg gataaggccg tgcaacaggc
    30361 tgccaaccag gcttttgcac cttgcgagat tgccttccat cagcagcttt caacgttcct
    30421 gattcacacg ttgggctacg atatgccggt ttcaggttgt cgcgaaggct cggtaacggg
    30481 caacctgatt acgggtgatg caggcagctt gaacattcgc gacggcaagg cggaatatgt
    30541 ttatccgcaa tgagtggcgt aaaaaccaat aaagacaaat taagatgatg tcggggaaga
    30601 tgcccgaccg acaagactat gcaaaatatg aaaaaccaag tacgcggatc aggcatggat
    30661 gcacgatcca atccggccaa tgtttcagac ggcctgcaaa acagttcgag ccatatcggt
    30721 accaacacgc gctaccgcct gaccaagctc ggcgaacaaa tggcgcgcct gcccatcgac
    30781 ccgaaaatcg cgcgcatttt gctggcggcg aagaaacacg actgtatggc ggaaatattg
    30841 gtgattgcgt ccgcgctgtc gattcaagac ccgcgcgagc ggccgttgga agcgcgcgat
    30901 gctgcctcca aggcacacga acgttttacc gacaagcagt ccgatttcct tgcctatctg
    30961 aacatttggg acagcttcca gcgtgagcgc gacaaaggtt tgtccaacaa gcagctggtg
    31021 cagtggtgcc gccaatattt cttgtcgcac ctgcggatgc gcgaatggcg cgagctacac
    31081 caccagcttg cccaaactgc gattgaaatg ggtttgacca ccaaagaagc cgctttcaga
    31141 cgttctcccg aagtcaggca gctcacgtcg tctgaaaatg caggcgacca agacctatcc
    31201 gccaaactca aacaaaaaca actggataag aaacaacacc gcgcccaaat ccgcgctgcc
    31261 aaagaagcgg gctacgaaca aatccaccgc gccctgctca ccggcctcat cgccaacgtc
    31321 ggcatgaaat cacccgacgg caacgactac accagcacgc gcggcagccg cttccacctt
    31381 ttccccgcct ccgccctgtt caaggcgaag cccaaatggg tgatggcggc agaactcgtt
    31441 gaaacgacaa aactttatgc ccgcgacgta gccgccatcc agcccgaatg gatcgagcag
    31501 gaagcgccgc acctcgtccg ctaccactat ttcgagccgc attgggaaca aaaacgcggc
    31561 gaagtcatcg ccagcgaacg ggtaacgctc tacggcctga ccgtcctgcc gcgccgcccc
    31621 gtgtcctacg gcagaatcgc ccccgaagaa gcgcgcgaaa tctttatccg cggcgcattg
    31681 gtggcacagg aatgcgattt gaaagcggac ttttttgtcc acaacaaaaa gctgattaaa
    31741 gaaattaccg aactcgaaca caaatcgcgc aagcaagacg tgctggtcga tgacgaagcc
    31801 ctgtttgcgt tttataacga acgactgccc gaaatggctt ggaaagacgc gcaaggcagc
    31861 gtttggggaa gtgaagattc cgtacggatt attgaatctg acaaagccga gaggtcgtct
    31921 gaaaatgagc gcaacgagtt tcgtaaaaac aagcgtaatg ggtctcgcca aaatgaaaat
    31981 cacggcaaca ccgtaggttg ggttgaaaac ccaacatcag ccgcaactgc aaaaactgtt
    32041 gggtttgaca acccaaccta cgctactcag caacccaccc cctcccccga gagggagggt
    32101 cggggagagg gcaaaacagt tgccgcacaa accaactttt ccgcaaccgc agcaaaccct
    32161 ctcccgcagg agagggaaca gagtgcctca gcttcaacgt tttcagacga cctgcgtcct
    32221 gcaaatctgc agcaaaccgc cccctccccc gtgggggagg gctggggaga gagcaaaaca
    32281 gttgccaccc aaaccaactt ttccgcaacc tcaacacttt cagacgaccc caaacccaaa
    32341 aagcagcctg cacctcaaaa aggccgtctg aaacccctac ccctcgccga catccgcacc
    32401 ttccaagcct ggctcaaaac cgccgagtgc gacaatccgc gcctgctgtt cctcagccgc
    32461 gacgatctga tgcaacacgc cgccgcacac attaccgaag aacagttccc caaattctgg
    32521 caaaccgcag acggcaaatt caaactttcc taccgcttcg agccgcacca tccgctagac
    32581 ggcgtgacca tgaccgtgcc gctgaccgtc ctcaaccgcc tgcacgcgcc atcgctcgaa
    32641 tggctggtgc ccggtatgtt gcgcgaaaaa atccagctgc tgattaaagc actgccgaag
    32701 caaatccgcc gcatttgtgt gcccgtaccc gatttcatca ccaaattcct cgaaagcaat
    32761 cccgaccgcc aagcgaccat cattccccag ttggcgcact ttatcgccaa aagcgccagc
    32821 gatatgcgga ttctcgagca aatcgaccaa gacgcatggg cggcgcaaga attgcccgaa
    32881 cactgctatc tgaatctgcg cattatcgac gacggcggac aagagcttgc cggcggccgc
    32941 aaactgcacg aattgcaaca acagctcggc caagccgccg ccgtcacctt ccgcgacaac
    33001 acccaagaat tcgagcgcga caacgttacc acatgggaca tcggaaccct gcccgaatcc
    33061 atcaaattcg cccgcggcaa acaacagctc accggctacc tcggcctgca aaaagaaaaa
    33121 gacggccgca tcgccctgcg cctgtttgac accaccgaag ccgccgaaca ggcacaccgt
    33181 caaggcgtca tcgaattgat gaagctgcaa ttaaaagagc aggtaaaaga cctgaacaaa
    33241 ggcatccaag gcttcaccca agccgccatg ctgctcaaac acatcaacgc cgacaccctg
    33301 cgcgacgacc tcacccaagc cgtctgcgac cgcgccttta tcggcgaaga cgagctgccg
    33361 cgcaacgaaa aagccttcaa agaacaaatc aaacgcgccc gtagccgcct gcccgccgtc
    33421 aaagaagccc tcagccgcta cctgcaggaa accgcagccg cctacgccga actcaacggc
    33481 aaactcggca aacatccatt gacccacctc ctaagactac gcctgcaaac cctgcttgcc
    33541 cccggcttcg ccacccgcac cccgtgggca caatggccgc gcctccccat ctacctcaaa
    33601 gccatgaccc tgcgcctcga aaaatacagc agcaaccccg cccgcgacgc agcccgcgaa
    33661 gccgatatac aggaattgga acaaatgtgg caggaaaaaa acgacggttt ggtgaaacaa
    33721 ggtctcccca tttcagacgg cctcgccgcg tttaaatgga tgattgaaga attgagggtg
    33781 tcgctgttcg cgcaggaatt gaagacgcct tatccggtgt cggtgaagcg gttgatgaag
    33841 gagtgggaga ggttgaataa gtagtgatat tatccaatac atttacagga aaatttctaa
    33901 cttacataaa aggtacaact caatgaaaag cgaaaataat tatgaacgta ttgttcaaaa
    33961 ttctaaaaaa ttccaacact ctcagaagaa tgagatgcag aaacgcataa cagatttgct
    34021 gcaacaactc acaaatggca ttcatgaacg cgaccaaatt atggcggttt ccctacttgg
    34081 tgcgattgcc ggacataata ctttcctatt tggaccgccg ggaaccgcga aaagtctgat
    34141 ttcccgccgt ttggcatgtg catttgaaaa tccgcaatac tttgaatact tgatgaaccg
    34201 tttcagcaca cctgaagaag tattcggacc tgtatctatt aaggcattga aagaagaccg
    34261 atatctccga aaaatcgaag gctatttgcc aactgccgat tttgcatttt tggatgaaat
    34321 ttggaaatcc agtcctgcaa ttttgaataa cctgctgacc attatcaacg agcatatttt
    34381 taaaaacggc gatgagaggt ttaatgtgcc acttaaagcc ctgattgcgg cttccaacga
    34441 aattccacct gaaaatcaag gtttagaggc actgtatgac cgctttatta tccgtctgct
    34501 tgtgccgccg attgaacaag aagagaactt caaccgtttg ctggatagca agccaagcag
    34561 cgacaaacct gaaatcagtg aagatttacg gattagttat catgagcttg ccaaatggcg
    34621 tgaacaactc cacaaagtca ggctaagtac agatacatta ctgattatta agtatatcag
    34681 agaagaatta gcagcccagt ttgataagtt aggcgtttat gtatccgacc gacgttggca
    34741 acgtgcagcc gtattattaa aggcttctgc tttttgcaat ggacgcaaag aaaccaatca
    34801 tagcgatgtg atcttgctga aatattgtct gtggacaaca cctgaaaatc gagagtcagt
    34861 ggaacaaatt gtgatgaata ccattaaatc ctgtgggttt gatacaggta ttgatctggc
    34921 tgaattagat agagaaaaag agagtttgga taaggaaata aataaggaac tttatcattc
    34981 acaagatgtt tatgagacgg ttgaaatgag tggaaaaaaa tattttccag tacaggctgt
    35041 tttcaaaaac gtacataata gtcatgctaa taaagaactt aagatttatg ttccatatag
    35101 caaatttcgt agtaaagaaa aattttatcc aatagatagt acaggaaatg aaattcaaga
    35161 aattgaatgt gaatttgatg ggcaaggttg ctgtaaattg gaattgaaag tgcggggtgg
    35221 tcactggaat gagcaatata agccattcaa atttaaacct aacgttttat tccataaggg
    35281 agataaaaaa caaagcatca atgataggtt gatttggtct ttatcacgtt ctgttatgga
    35341 aatcaataaa gcattattag atgtattgaa attagtcacc aaaaaacatg cagaacatca
    35401 gcaattccta caaagccctt ttattaccca agatgatatt gatattgcgg tatcaggaat
    35461 tttagaccaa atcgacaaac tcaatttacg gattaaagac tgcgaacgtt tggagtcttt
    35521 atgtcgttaa atagcaaatt atccgaacaa ataagatgga ttaaagcttc tctacaaata
    35581 gattatgcag atttatttaa acagcatggt tcgttaaatg gacagattca gcaacgttta
    35641 tcagattggc aatatcagac caaactggct ttgttgcaga aaaacccgtt tgtattacat
    35701 gaagaaaagc taaaaaaaca ccaacaaaca gaaattttta atcaacagtc tgttgatgat
    35761 tatcggtatt tttgcaaatt agcaggcgtg ccatttgaaa aagaattctg gcagcaggaa
    35821 ttaacacaaa gcaaaaattc aaaacccgat aaacagaatt tgcctttgaa attgctaaca
    35881 gaaaaatggc agcagcaatt agactatgcc aaatctcaat ggtatttaga gcagcttaat
    35941 caattacgac aggaatttat agacaagtta aaacaggaac tcgacactat ccgacagctt
    36001 gcagaacagt tagagcaatt tggttttgaa gcagggctgt ggtttgataa cagtatcggc
    36061 aatttaagcc agcaaaatat tgaagaaatg aaacgttggc tgaattactt gacccaagat
    36121 aagaatgccc aacagattgc cgaattacta ggcaaaatgc gccaaatcga acaatccgaa
    36181 aaaatcgaac aggtaaaaca gactgtttac atccaaaatc cacaaattga tataaattcg
    36241 cgtgaagaaa ttatcggatt acgtttaggt aaagatttag agtatgtatt gccgtctgaa
    36301 ttggctttaa tggcagatga agaaacgtcg attttatttg atttgaagtt tttggaatcc
    36361 aaattaatgt gttttgaatt gcaaggaacg acctattgcg atgccccaac tgaaattata
    36421 gttgaacaaa aaagtcaaga ggatgaaaag ctcggaccaa tgattttatg tgtagatacc
    36481 agtggttcaa tggttggact acctgaaaat attgccaaag caatggcttt atttttagga
    36541 gcaaaagcta aaagcgaaaa ccgttcttgt tttgttatca atttttccac tggaatagaa
    36601 acttttgaat taacaagtaa aacagggatt tccaatttga ttgctttttt acgtcaatcc
    36661 tttcatggtg gtacagatgc tgccccagct ttacgtcatg cgctaaaaat gatggagcaa
    36721 gaaagctatc aaaaggcaga cttattaatg atttccgatt ttgtgatgaa cagcttgcct
    36781 gatgatttgt tggcatcaat cgaaatacaa cgagaaaccg gtaatcagtt taattctttg
    36841 gttattggtg atgcttttat gagtaaacgc ctaaaaacac attttgatcg tgagtggata
    36901 tacaatccta atgttcagac aattcaagaa ttagtacagt tcaagaaaga tattttcaat
    36961 aaacaagttg tcaactcata acactatcct actcatatgt atttttggaa gataccaccc
    37021 tatacaactt ggaatccata cgcagaatat gtgtggactt tctaaactac tcccctttcc
    37081 accctaaaaa aggtcgtctg aaacccattt tcagacgacc tttttccact tcgcaaaaca
    37141 agaattccca cccctctccc caaacgtcgc ccgaaccagt aaactcccca tatttcaaca
    37201 acttcttgga agcaaaccat gtccggtatc tacctacctc gcctatttcc gccccacatc
    37261 gtcgaacgcg gccttttgta ttttcagcag ggcaaggttc gcgatgtccg aaaaacttcc
    37321 gccgggcgtt atcgggcctg gcgccgtttg ctgccggcgg tctgaataat ggtggcgtca
    37381 acgacggcag cggatgcttt ctctactttt aggttttttt cggtcagttg gcggttaatc
    37441 agtttgagca attcggacag ggtgtcgtct tgcgccagcc agttgcggta acggcataag
    37501 gtgctgtaat cggggatgct cagttcgtca aagcggcaaa acaggttgaa gtcgatgcgg
    37561 gtgatgaggc tgtgctcgag ttcgggatcg gagaggctgt gccattgtcc gagcagaacg
    37621 gctttgaaca tggacaacag cgggtaggcg ggacgaccgc ggtggtctcg gaggtaacgg
    37681 gttttttgac ggttcaggta ttgttcgatc ggttgccaat caatcacctg gtccaacttc
    37741 aatagtggga aacggtcgat gtgtttggca atcatggctt gggcggtttg ctgaaagaag
    37801 gtgctcatga gaaatctcct aaatgtcttg gtgggaattt aggggatttt ggggagtttt
    37861 gcaaaggtct cagtcttttt aaaccacagc ccttcccaac taaaccaaaa ggtcgtctga
    37921 accctatttt cagacgacct tttgccactt tgtaaaacaa atcttcccac catcctctcc
    37981 ccaaacatcg cccgaaccag taaacttctc atatttcaac aacttcttgg aagcaaacca
    38041 tgccccatat ccacccgctc cacctgttcc cgccccatat cgccgaacgc ggactgctct
    38101 attttcagca gggcaaagtt ggtgctgtcc aaaaaacttc caccgggcgt tatcgggcgg
    38161 aggtatgcgg tttggaaaac tattgggtat ggctgaagct ggatagtgat ttgtatatta
    38221 aagacgaaag ctgcgattgc ccttatgtct aaaggtgcaa acatacctta aattactata
    38281 ttacataggc aaaatacaag cctataacga attggaaaca aaatgccgtc tgaaagcatt
    38341 ataaaatctg ttcacctttt cagatgagta atgtacaccc ctatacaatt tttgctacta
    38401 taccccataa atccacggct aaagatatcc ttattatgtc ctatgattta tcgaaacgac
    38461 ttgtaatcgg cttagcatca agtgccctat tcgacttatc cgaatcggat aatatattta
    38521 gaatggaagg ggcagaaacc tataggcaat atcagagaga aaaacaaaac catcccctaa
    38581 aaaaggcgtt gtctttccat ttattaaaaa acttctgtca atcaatgaaa taaacccaaa
    38641 cgacccaaca attgggttta ttcttttatc cagaaacaat ccagatacag attacgagtc
    38701 ataactatag gcttaatatt acacgattct cattccatca aggcggaaaa ccgcacaaat
    38761 actgaaacac tatcgatcga tttgtaaaca agcctactta agtaacttgc agtccttatc
    38821 atttccttta aaataatcca gcccgtcact acacgaactg gcggacttct tgcaaataaa
    38881 ggttactaga ttttcattca tcttaataat aaaaggattt ttatctttat ctatggctac
    38941 cgccttcaat atgaatttac tgtctaaggc cccgcgcgcg attccattca aacggataca
    39001 aaagccttct gcctctttaa tcggcaaact tggccatttg gtagatgttt gtttaaatct
    39061 cccattctgc agataaaact tttccataaa atgtgcattt tctaacaagg ctgcccgcac
    39121 tgcatttatc tttgctttct caacataatt gcgatagctc ggataaacaa ttaaagcaag
    39181 tacagacaat atcaagacca ctgatattaa ttcaaccagc gtaaaccccc gattatcagt
    39241 cattacttta cttccaataa gaacagatta ttcaacatat ttctttgaac agacttacta
    39301 tcccattcag cagtatgcat atttcccac
//
LOCUS       DAWGJV010000003        33053 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWGJV010000003 DAWGJV010000000
VERSION     DAWGJV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.003"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(90..1037)
                     /locus_tag="WHQ94_000085"
     CDS             complement(90..1037)
                     /locus_tag="WHQ94_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ2127440.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1052..1459)
                     /locus_tag="WHQ94_000086"
     CDS             complement(1052..1459)
                     /locus_tag="WHQ94_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ2127441.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIITHP"
     gene            1540..2199
                     /gene="ung"
                     /locus_tag="WHQ94_000087"
     CDS             1540..2199
                     /gene="ung"
                     /locus_tag="WHQ94_000087"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ2127442.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            2240..2758
                     /locus_tag="WHQ94_000088"
     CDS             2240..2758
                     /locus_tag="WHQ94_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ2127443.1"
                     /translation="MELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDV
                     TDFNDEDKLGKGKEMIDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESG
                     KSKGQFYTPAEVSRIMAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTV
                     RKKMWQPRPLPV"
     gene            2831..3304
                     /locus_tag="WHQ94_000089"
     CDS             2831..3304
                     /locus_tag="WHQ94_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ2127444.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            3652..4596
                     /locus_tag="WHQ94_000090"
     CDS             3652..4596
                     /locus_tag="WHQ94_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2127445.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            4738..4872
                     /locus_tag="WHQ94_000091"
     CDS             4738..4872
                     /locus_tag="WHQ94_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ2127446.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(5162..5677)
                     /locus_tag="WHQ94_000092"
     CDS             complement(5162..5677)
                     /locus_tag="WHQ94_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127447.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(6143..8065)
                     /locus_tag="WHQ94_000093"
     CDS             complement(6143..8065)
                     /locus_tag="WHQ94_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2127448.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEIARLTDIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(8142..8537)
                     /locus_tag="WHQ94_000094"
     CDS             complement(8142..8537)
                     /locus_tag="WHQ94_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ2127449.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYLEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            8710..10017
                     /locus_tag="WHQ94_000095"
     CDS             8710..10017
                     /locus_tag="WHQ94_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ2127450.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            10010..10444
                     /locus_tag="WHQ94_000096"
     CDS             10010..10444
                     /locus_tag="WHQ94_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127451.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(10503..10772)
                     /locus_tag="WHQ94_000097"
     CDS             complement(10503..10772)
                     /locus_tag="WHQ94_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ2127452.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(10954..13416)
                     /gene="lon"
                     /locus_tag="WHQ94_000098"
     CDS             complement(10954..13416)
                     /gene="lon"
                     /locus_tag="WHQ94_000098"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ2127453.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPIAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLTQRQQILEISEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            13667..14770
                     /locus_tag="WHQ94_000099"
     CDS             13667..14770
                     /locus_tag="WHQ94_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2127454.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFVDLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMI
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(14798..16543)
                     /gene="recD"
                     /locus_tag="WHQ94_000100"
     CDS             complement(14798..16543)
                     /gene="recD"
                     /locus_tag="WHQ94_000100"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ2127455.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAIRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGGLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSRGLLYTAITRAREKFVFFGGEEAFRQAAVTVKTR
                     QTALGSMLERVFSQE"
     gene            complement(16611..17306)
                     /gene="lolD"
                     /locus_tag="WHQ94_000101"
     CDS             complement(16611..17306)
                     /gene="lolD"
                     /locus_tag="WHQ94_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ2127456.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEILAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSAHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(17299..18546)
                     /locus_tag="WHQ94_000102"
     CDS             complement(17299..18546)
                     /locus_tag="WHQ94_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ2127457.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            18751..19029
                     /locus_tag="WHQ94_000103"
     CDS             18751..19029
                     /locus_tag="WHQ94_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127458.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(19085..19702)
                     /gene="recR"
                     /locus_tag="WHQ94_000104"
     CDS             complement(19085..19702)
                     /gene="recR"
                     /locus_tag="WHQ94_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ2127459.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVHHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEVANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(19769..21307)
                     /locus_tag="WHQ94_000105"
     CDS             complement(19769..21307)
                     /locus_tag="WHQ94_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ2127460.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLLGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKLSFEQEKAAVENELKMKKAVADFN
                     KAKEKLSDDAFNHPASLAEAAKNSSLKVETQETWLSRQDAQMSGLPENLINAIFSDDV
                     LKKKHNSEVLSINNEIAWVVRAKEVREEKTLPFAEAKDAVRQAYIRAEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPTNGKPAYVRLTGLP
                     APVIVEVQAITPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GGGQ"
     gene            complement(21361..21735)
                     /locus_tag="WHQ94_000106"
     CDS             complement(21361..21735)
                     /locus_tag="WHQ94_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ2127461.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQMPSEAEICS
                     WLEQVPLETLLNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            21885..23513
                     /locus_tag="WHQ94_000107"
     CDS             21885..23513
                     /locus_tag="WHQ94_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ2127462.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGSRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            23598..24851
                     /locus_tag="WHQ94_000108"
     CDS             23598..24851
                     /locus_tag="WHQ94_000108"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ2127463.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECWAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            25074..25382
                     /locus_tag="WHQ94_000109"
     CDS             25074..25382
                     /locus_tag="WHQ94_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127464.1"
                     /translation="MTLKTDLLLKINNEDYQRLILKHISEFSEGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            25464..26495
                     /gene="ruvB"
                     /locus_tag="WHQ94_000110"
     CDS             25464..26495
                     /gene="ruvB"
                     /locus_tag="WHQ94_000110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ2127465.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(26518..27246)
                     /gene="rpe"
                     /locus_tag="WHQ94_000111"
     CDS             complement(26518..27246)
                     /gene="rpe"
                     /locus_tag="WHQ94_000111"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ2127466.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            27392..27826
                     /locus_tag="WHQ94_000112"
     CDS             27392..27826
                     /locus_tag="WHQ94_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ2127467.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(27839..28702)
                     /locus_tag="WHQ94_000113"
     CDS             complement(27839..28702)
                     /locus_tag="WHQ94_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ2127468.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(28710..29327)
                     /locus_tag="WHQ94_000114"
     CDS             complement(28710..29327)
                     /locus_tag="WHQ94_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ2127469.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(29490..30104)
                     /locus_tag="WHQ94_000115"
     CDS             complement(29490..30104)
                     /locus_tag="WHQ94_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ2127470.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            30310..32082
                     /locus_tag="WHQ94_000116"
     CDS             30310..32082
                     /locus_tag="WHQ94_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ2127471.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLKKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            32079..32735
                     /locus_tag="WHQ94_000117"
     CDS             32079..32735
                     /locus_tag="WHQ94_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ2127472.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPNVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQSTQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         7784 a         9094 c         8370 g         7805 t
ORIGIN      
        1 ttgaagcgga atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac
       61 agagcttcag acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt
      121 ttcataccgg cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta
      181 ttgctttctt tggcaagatt gttgaacgca gcgacgtatt gttccgcaat cttcagattg
      241 accgcatccg cgccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca
      301 ttggcttcgg caacaaggcg cagggattcc gcttcgcctt tggcgcggtt gatgcgggcg
      361 attttctcgg catttgacgc attgaccgcg gcctgggcct cgccttcgga ttgttggatt
      421 tcggcttcgc gctgaccact ggcaaggttg atttgttcga ttttacggcc ttcggattcg
      481 gcgatacggg cgcgtttttc gcgttcggca gtaatttgcg cctgcattga gcgaaggatt
      541 tcttgcggcg gaaccaaatc cttgatttcg taacggagaa ctttcacacc ccaagctccg
      601 gcggcttcat cgagggcgga aacgacggtg ctgttgattt cgtcgcgttc ttcaaacgtt
      661 ttgtccaact ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc
      721 gccataatgt agttgctcga accgtatgag gcgagtttgg gatcggttac ttggaaatag
      781 atgatgccgt caaccgtcag ctgggtgttg tcgcgcgtga tgcagacctg gctgggtacg
      841 tctaaaggga tttctttcag cgaatgacgg taggcgacgc ggtcgataaa gggaatcaaa
      901 atattcaaac cggccgtcaa ggcgcgatgg aaacgtccaa gcctttcgac aacgtggact
      961 tcctgttgtg ggatgacaac aaaggatttg aaaccgaaaa cggcgacggc taccaacaag
     1021 ataatgaaaa attccataat tcctccgagt gttaagggtg tgtgataata agaaggttgc
     1081 cttccttgcg gacaatgagg gcgcgcgttc ccggttcaaa cacatcctgc cccgtatttt
     1141 gcgcctgcca gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gcgtgccgga
     1201 ggatttcggc atattgcccg gcatccaaat cctgatatga atccgtttca acttttccca
     1261 cggcggtctt ggcgtgtacg aaccaaatac ccagcgcgga aagcagagcg gcggtcaaga
     1321 cggcggcagg cgtgctgccg gtcagcccgt aagcaatgcc cgaacccgcc aaagccgcgc
     1381 tgacaaccaa aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcgg
     1441 caacaaacca tacagtcata catttcccca caaagcgcgt cgtttgacaa aatcacgcga
     1501 tatcagcagt atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca
     1561 ctgggcaacg aaaaacggca accgtatttt caggaaattt taaatgcagt caggcaggaa
     1621 cgtttgtcgg ggcaaatcat ctatccgccg gcggaggatg tgttcaacgc attccgcctg
     1681 acagcgtttg accgagtcaa agtcgtcatt ctcggacaag acccgtatca cggggcaggg
     1741 caggcgcacg gtttggcatt ttccgtcagg cagggtgtcc gcataccgcc gtctttactc
     1801 aatatctaca aagagttgga aaccgacatc aacggttttt ccattcccga tcacggctgc
     1861 ctgacggctt gggcggagca gggcgtattg cttctgaaca cggttttgac ggtgcgtgca
     1921 ggacaggcgc attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg
     1981 cagcttgcga cacaccgcaa gcaccttgtc ttcatgttgt ggggtgggta tgcacaacaa
     2041 aaagggaggc tgatagacag tcaaaatcat ttgatattga ccgcaccgca tccgtctcct
     2101 ctgtcggcat atcgcggttt tttcggctgc cgccattttt cacaggcaaa cagctatttg
     2161 agtcaacacg gtatcgaacc gataaactgg aagctatgaa tgccgatata gccgttgccg
     2221 ccggcgtgtt aaaatcgcga ttgagctgca cgccggtacg acttttgacg acatcgtcaa
     2281 actcaaaaac accgccgaca tcggcgaccg cctgaataag attatcgccc aaattgccga
     2341 agccaacgac ttaaaaggcg tgatcgacgt taccgacttc aacgacgaag acaaactggg
     2401 taaaggtaag gagatgatcg accgtttgag caggcttgtc ggcattttta aaaagctcaa
     2461 cctttcttcc aaccaagccg aagacgacga tttgttaggt gatgcctacg aatacctgat
     2521 gcgccatttt gcgaccgagt caggcaaatc caaagggcag ttttacacgc ctgccgaagt
     2581 ctcccgcatt atggcgaaga ttatcggaat cagcgcagat tgccgtccag caccagcgtt
     2641 tatgacccga cctgcggctc gggttcgctg ttgctcaaag ccgccgccca agccggcagc
     2701 caaatcagcc tttacggtca ggaaaaagat gtggcaaccg cgtcccttgc ccgtatgaat
     2761 atgattttgc acaacaacga aaccgccgaa atcaacaccg ggaacacctt gtccgattcg
     2821 tctttccgtg atgaaaacga cgggcttaag accttcgatt ttgccgttgc caatccgcct
     2881 tatcccgccc gaaaaaaacg gcgattacgc ctttttgctg catctgctca aaagcctgaa
     2941 accaagcggc aaaggtgcga ttattcttcc gcacggtgtg ctgtttcgcg gcaatgccga
     3001 agcgcgtatt cgcacggaat tgcttaacct tgaccttatt aaaggcatta tcgggctgcc
     3061 tgccaacctg ttttacggca cgggcattcc tgcctgcatc atcgtcatcg acaaagaaca
     3121 cgcccaaacc gcccaatttg ccgaagaggg aacaaaccaa gttatcagcg gcggcagcgt
     3181 gtttatgatt gacgcatcgc gcggcttcat taaagacggc aacaaaaacc gtctgcgtga
     3241 gcaagacatt cacaaaatca tcgacacttt cacaaacctc gttacagccg tatggtgcat
     3301 ttaagcgaaa tcgcagcaca agattacaac cttaatctgc ctcgctatat cgacagcgga
     3361 gaagtcgaag acctgcaaaa tctcgccgcc tatctatctt tatggcggca tacctgcgca
     3421 cgatatggac gcattggaag cctattggca agttttaggc cgtatgaaaa acgagttgtt
     3481 tgccgaacac gatggccact ttaccactat acaacggaat cgattgcaaa tctttcccac
     3541 tctcaacagc ttaaaatcct gcgggattgg tgtggaattt agggctaatc tagtacagcc
     3601 ccaaatttaa tccactataa aatcgaaagc agccaaatca aagcccatat attggcgcac
     3661 cccgattacg ccgccttcaa agccggacac ctggcaaagt ttgccgcgtg gcacactcaa
     3721 aacgaccttg ccgccatcca accgggcagg cttatccgga aatggagcga aagcctgctg
     3781 gacgcgttca aacccggcag cctgattgaa gaatacgatt tctaccaaat cctgacggac
     3841 tactgggcgg aaaccctgca agacgatgtt tatctcatcg cccaagatgg ctggaaggcg
     3901 gttaaaaacc tggccgaaat caccaaagaa agcgatgaag ccgcgaacct gaccgtcgtc
     3961 tttgaggaaa ccgaaaccga caaaaaaggc aaagccaaaa ccaagcgcat cagcaaaaaa
     4021 taccgcagcg aagtcatcgc ccccgagctg gttgcccgcc gctacttttc agacggcatc
     4081 gccaagctgg aagaaaaaca aagcgagctg gaacgcctaa gccaagaatt ggaaaaccac
     4141 atagaagaac acggcggcga agagggtgcg ctgaacgacg tattggatgc aaaaggcaaa
     4201 ctttccgcca aacttctgaa aaccgcattg gaagaaagcg gcatagaaga aggcgaacgg
     4261 gctgttttac aaaccaccca aacactgatg acgcaggaaa aagccgcgaa agacgcagtc
     4321 aaaacccaaa tcgaagccct gaaccttgcc gtattcaaac aattcggccg actttccgaa
     4381 gccgaaatca agcagcttgc cgttcaagac aaatggcttg ccgatttaca aagtcgaatc
     4441 gaaaatcgct tggaaaacag tattcagcag cttatcagcc gcttgaacac gctggaagac
     4501 cgctaccgca gcccgatggc cgagcttgcc cgagaagtgg aaaagtggca aagcaaagtc
     4561 aatgcccacc ttgaaaatat gggttttgga ggctgaaatg gcggcacaga caggctataa
     4621 ggcgagcggg ttttgagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga
     4681 catttagggg atcgcggttc gggtgtccgc accgcttaat acgtcgtcgt ccacgaactg
     4741 acccatttgc tcgaacgcca tcgcaacgcc cgttttatgg cgcatatgga caactttctc
     4801 ccaaactggc aaagcatcaa acaacagctt aatgccttgg agttatttgc acaaatatat
     4861 aatttaacat aatatacatt atgcgaacta tcggaaacag ttgtacgtgt ccctgtggtc
     4921 tttccaagta ggaaaattaa agtatggcca atgcggctga atgtatagcc cggagcatcc
     4981 gcgtatccga aggtactgaa cgacatgccc tgcatccctt tcgacgcttc catccagccg
     5041 gtattgggaa atacgttgac cttcaatata atatacaggc agccgacaca caggatatgc
     5101 cgctgctttt tcatcatttc ttctgtcaaa tccttggaac ggtcgatttg aaaacacggc
     5161 tttacatacg ccccagttcc ctttgcaggg cttgaatatt ttgctgcctg tccaatacat
     5221 tgctttgtaa tgcatttatt tcttgatggt tgatgttgcc gccctttgcc agacgtgctt
     5281 gtgataacat tttttgggct tcagtcaggg ctttgcgttc gttgctcaat tctgcttcga
     5341 gaatggagcg tctgctgttg tttgagggtg cttgttgcgg cggcggcgta ttggattttg
     5401 ccggcttgga tactgttttg accggggctt tatatttgac aacctgtccg ccgtttgacg
     5461 gtgatgatac cggttcgggc gtttggggcg ggatatagcg ttcgctgctg tagttgccga
     5521 ttgggggcaa atcggttgag tggcagcttt tggacggctt ggtggtgtaa acggtttctc
     5581 cgttgattgt gcaggtgtag attttggccg cattcgcacc caatgggctt gaaatcaggg
     5641 aaaagttgat taggattaag gggagttttg atttcataat gtgcttgatg ctcggataat
     5701 tatttgattt tttggtattt tttataatgc tttcttggcg ttttacgggc ttttcttttc
     5761 ggttgtttgc ttttgatttt ctttcggggc tttggcagcc tgcttgttct ttttcccggc
     5821 aggttttttg tttttctttt tactttctat gtgtattttg gcttcttaac tgagtttttt
     5881 aattttcagg cggtatccgc ctccctgatg gctgctgatt ttaggtaaat ccgcatcggc
     5941 gcacgatcct gctggggctg atgtatatac gcttttgcca tttgagttgc aatggtatac
     6001 ggaggcttgc gccgccgcgc cggaaaggga caggagaccc aaggcggcaa aaagtctgat
     6061 gtttataccg gtaaaaggcg gtaataacgc ccgaattata ccgttattgg caggcaaaga
     6121 taagcaccct gcccgcgctt ctttatcgct cggcaaactg tttctcgatt tcagtttcaa
     6181 tccgttccaa ttcttcttga caagccagcc aaacctcttc gatttgggca agttgtgttt
     6241 tgacttttgc cagctgggat aaggtatttt gcaatttttc tttgtttgct tcaagataag
     6301 cgtcttcttg tgctaaaaat gcttcacagg ccgtctgaat atcagtcaat cgtgcgattt
     6361 ctttttcggc acggtctatt ttctgctgta tcggcttgcc gcgtcgggct ttttcctgac
     6421 ggatttgcgc ttcgatgcgc ttggtgtctt tgcggctttg gctttgtgcg gatgctgcgg
     6481 gtgcggcggc ggcgttttcc tgtgccaaac gccattggcg gtaatcgttc aaatcgccgt
     6541 cgaagttctt cagacggcct ttgtcgatca ggaggaagct gtcggtagtc gcttcaagca
     6601 ggctgcggtc gtgcgatacg gcgattaatg cgccttggaa actttgcaac gcgagcgtta
     6661 aggcgtggcg catatccaag tccaaatggt tggtcggttc gtcaagcagc aacaggttcg
     6721 gcttttgcca gataatcatg gcaagggcaa ggcgggcttt ttctccgccg gaaaaaggtt
     6781 cggttttctg taacgccata tcgccaacaa aattgaagcc gccgaggaaa tttcggattt
     6841 cttgttcgcg tacttcggga gaaagctgct gaatatgcca aacagggttt tggtcggcgc
     6901 ggatggtatc gagttggtgt tgggcaaaat agccgatatt gagtttttcg gaacggacga
     6961 tgctgccaga gagcaaatcg attttgcccg ccaaggcttt gataaatgta gatttaccgc
     7021 tgccgttgac acccaataaa ccatagcgcg cgccgctttc cagcgacagg gtaatgtcgt
     7081 gcaaaacagt tttgccttcg taacccaaat ctgcgtgttc cagcttcagc aaaggattgg
     7141 gcagatgctc gggattgtaa aactcaaagg aaaactcgct gtccaaatgc gcgggagcga
     7201 tgcgttcgag ctttgccaaa gccttcatgc ggctttgcgc ctgaacggct ttggtggctt
     7261 tggctttgaa gcggtcgata aaggattgca gatgtttgat ttgcgcctgc tgtttgacat
     7321 aggcggcttg ttgctgcgcg agacgctgcg cgcgttcgtt ttggtaaaaa tcgtaattgc
     7381 cgccgtattg cgtgagcttt tgctgggaca attcgatggt ttgggtggtt gccgcattga
     7441 ggaaatcgcg gtcgtgggaa atgatgattt gcgtgcaggg taaagaggca aggtggtttt
     7501 ccagccacaa gacggtttcc aaatccaagt ggttggtcgg ttcgtccaaa agcagcaaat
     7561 cggcgcggca aatcagggct tgcgcaagat tcaagcgcat acgccagccg ccggaaaagg
     7621 atttgacggg gcggctgtgt tcttcttgcg aaaaacccag cccgttcaac aattttgccg
     7681 cgcgggccgg cgcggtataa gcgtcgattt cttccaattt ggcatgatat tccgcctgtt
     7741 tcataccgtc gttttgcgct tccgcctgcc tcaatgccgt ctgaagggtc tgtaactcgg
     7801 catcgccctg caaaacgtaa tctaaagcgg aaatatccaa atcgggcgtt tcttgggaaa
     7861 cggaagcgag ccgccagttt ttcggaatcg agacatcgcc gccgtcctga gtgatttcac
     7921 ccttgattaa ggcaaacagg ctcgatttgc ccgtcccgtt tttgccgatc aaaccgacgc
     7981 gctgaccggg attgacggta gcgttggctt tgtcgagcag gactttcaaa ccgcgttgca
     8041 gggtgaggtt tttgatttca atcataacgg aaacatcgtc gggcgggaaa agcccgtatt
     8101 ttacctgaaa gtcagtgccg atgccgtctg aaacgggaaa tttacggctg aagccaagcc
     8161 caagccctgc gcccttccga gtgcaggaaa accaatgtcc tgaatgccga atcggtattc
     8221 atacattcca cgccgattcc gattcgagaa aaatccgcca tgattttggg atggataaac
     8281 tcctgagccg cgcccgtccc gataatcaat atttccagat agtcaacagg tttgatgtcg
     8341 gacaacaggt tttccggagt cagatcggac aaggttcggc attgcgacag gcagaccgaa
     8401 tccttatgta caagcacggg tttatggaaa ctttgccccg ccagccggat tccgcccgca
     8461 ccgcattcat attccgcaaa ctgtccgtct atcggatttt cttcaaacaa cattttttta
     8521 ccccgttgcc gcatcatcta caccgaaagg ggtgcaaaat caggcgaatt catgtaggat
     8581 tggcagattt catctgaccc gcctgccgat ttcagacggc atttgattca aagtgcggca
     8641 caattatatc ggcagcggat attttcgtct ttcaatattt acatttcagt cggcttacaa
     8701 ggagacacaa tgaagccagt aaacatcggt cttttaggtt tgggtacggt cggcggcggt
     8761 acggctgccg tgttgcggga caacgcggag gaaatttccc gtcgcttggg gcgcgaaatc
     8821 cgtatttctg ccgtgtgcga tttgagtgaa gaaaaagccc gacaaacctg cccgtccgca
     8881 gcctttgtca aagatccgtt cgaactggtc gcacgtgaag acgtcgatgt cgtcgtcgaa
     8941 ttgttcggcg gtaccggcat tgccaaagat gcggtgttga aagccattga aaacggcaaa
     9001 cacatcgtta ccgccaacaa aaaactgctc gccgaatacg gcaacgaaat cttcccgctg
     9061 gcggaaaaac aaaacgtcat cgtccaattt gaagcggcag tagcgggcgg tatcccaatc
     9121 atcaaagccc tgcgcgaagg tttggcggca aacaggatta aatccatcgc cggcattatt
     9181 aacggcacca gcaacttcat cctctccgaa atgcgcgaaa aaggcagcgc gtttgccgat
     9241 gtactgaaag aagcgcaggc attgggttat gccgaagccg atccgacctt cgacatcgaa
     9301 ggcaacgatg cgggccataa aatcaccatc atgagcgcac tggcattcgg cacgccgatg
     9361 aacttttccg cctgctacct cgaaggcatc agcaaactcg acagccgcga catcaaatac
     9421 gccgaagaac ttggctatcg catcaaactg ttgggcatta cccgcaaaac cggcaaaggc
     9481 atcgagctgc gcgtccaccc taccctgatt cccgaaagcc gcctcttggc aaacgtcaac
     9541 ggcgtgatga acgccgtgcg cgtcaacgcc gatatggttg gcgaaacctt atattacggc
     9601 gcgggcgcgg gcgcattgcc gaccgcttcc gccgtggttg ccgatatcat cgacatcgcc
     9661 cgcctggttg aagccgatac cgcccaccgc gtaccgcatc tggcgttcca acccgcgcaa
     9721 gtccaagcgc aaaccatcct gcctatggac gaaattacca gcagctacta cctgcgcgtc
     9781 caagccaaag acgaaccggg cacgctgggg caaatcgccg cgctgttggc acaagaaaac
     9841 gtgtccatcg aagcactgat tcaaaaaggc gtgattgatc agaccactgc cgaaatcgtg
     9901 attctgaccc acagcacggt cgaaaaacac atcaagtcgg caatcgcagc catcgaagca
     9961 ctggattgcg tggaaaaacc gattaccatg atccgcatgg aaagcctgca tgactgagcc
    10021 gaaacacgaa atgctgacga aagagcaggt tgccgcgcgc aaaaaagcaa aagccaaaat
    10081 ccgcaccatc cgcatttggg cgtgggtcat tttggcgttg ctcgctttaa ccgccctgct
    10141 ctcccaatgc gcgatgtcca aaccgcaggc aaaacagaaa attgtcgagt cttgcgtgaa
    10201 gaatattccg tttgccgaaa aatggcaaaa cgatttgcgg gcccgcggtt tagattcaaa
    10261 caatacccgc ctcgccgtcg actactgcaa atgtatgtgg gagcagcctt tggacagatt
    10321 gagcgagaaa cagattagat ccttcggcaa actcggcgca caagaacagc ttgacctgct
    10381 cggcggcgca aatgcctttg aagcacgtga caagcagtgt gttgccgatt tgaaatcaga
    10441 ataatgtgga ccgataaaaa agccgattct ttaaagaatc ggcttttttc ataaaaaacg
    10501 gcttacagtg cgtctttcaa agctttgccg gcgcggaatt taggcgtttt ggcggcggca
    10561 atggtcagag gctcgccggt tttggggttg cggccttggc gttccgcacg ttcgcccacg
    10621 tagaaagtac cgaaaccgac caaagtaacg gtgtcgcctt gtttcagggc ggtggttact
    10681 gcattggtag tggcatccaa agctttttgt gcggcggctt tggaaatgtc ggcttcttga
    10741 gcaatcgctt cgatcaattc agacttgttc acaatcagtc ccttcctgtc ttaaaaaatg
    10801 atgaaatgcc cgaatactcg gggtttgtac tgcttgagca actttcgctt tatagcaatt
    10861 ctgaaattgc cgtgtcaagc aaaaaatacg gaatcaccct atttgacagg ctttcaggac
    10921 gaaaccgcat ttttacaaca catttcctgc gtttcaatgt ttggttgccc tgctgcgggg
    10981 ttttggtttt gaagcggatt ccgccgccgc ttccgcacca gaaggttctg cccaaggctc
    11041 aggctggctt tccaaaccca gagccaatac ctcgtctatc catttgaccg gatggatggt
    11101 caggccggtt ttcacgtttt cagggatttc ttccaagtct ttgaggttgt cttttggaat
    11161 taaaacgtgt ttgatgccgc cgcgaagcgc agccaacagt ttttccttca gaccgccaat
    11221 cggcaggact tcgccgcgca gggtaatttc gcccgtcatg gcaacatcgg cgcgcaccgg
    11281 gattttggtg aatgcagaca ccatcgccaa agtcatggca atgcccgcac tcggaccgtc
    11341 tttcggcgtc gcaccttcgg gaacatggac atggatgtct tttttctcgt aaaaatcagg
    11401 agccaagccc actgattccg cgcgggagcg gacaaccgac cacgctgcag acacggattc
    11461 cttcatcaca tcgcccaact ggccggtgca ctgaatcacg cccttacccg gcaatgctgc
    11521 ggcttcgacg gtcagcagtt cgccgccgac ttctgtccac gccagaccgg ttacttgtcc
    11581 gatacggttt tcgctttcgg caacgccgta atcgaagcgg cgcacaccca aatagtcgtg
    11641 cagatttttc tcatttactt taaccgcttt aggtttggct ttgctggttt tcttggtttc
    11701 agacaacctc ttcttatctt cgtccaaggt aatctgcatc accaccttgc ggcagatttt
    11761 ggcaatctcg cggtcgagcg aacgcacgcc cgcctcgcgg gtgtagtaac ggataatatc
    11821 gcgcaccgcg ctttcttcga ttgccaattc cccttctttc acgccgttgc gcttcatttg
    11881 cttcggcacg aggtactgca tcgcgatatt gattttttcg tcttcggtat agccggacag
    11941 acggatgatt tccatacggt cgagcagcgg ggtcgggata ttcaggctgt ttgaagtcgc
    12001 aataaacatc acatcgctca aatcgtaatc cacttcggca taatgatcgg cgaacttgtt
    12061 gttttgctcg ggatcaagca cttcgagcaa cgcgctggcg ggatcgcccc ggaagtcgct
    12121 acccaatttg tcgatttcgt cgagcaggaa caatgggttt tttacgccgg cttttgccat
    12181 attctgcaga atcttaccag gcatggagcc gatataggtg cggcggtgtc cccggatttc
    12241 gctttcgtcg cgcacgccgc ccaaagccat gcggacatat ttccgccccg ttgctttggc
    12301 gatggattcg cccaaagagg ttttgcccac gcctggaggg ccgaccaggc acagaatcgg
    12361 gcctttgagt ttgtccatac gtttttggac ggcgaggtat tccaaaatcc gttctttgac
    12421 tttttccagg ccgtagtggt cggcatccag caccagtccg gctttggcga tgtctttgct
    12481 gacgcgggat tttttcttcc acggcagttc gagcagggtg tcgatgtagt tgcgtacgac
    12541 ggtggattcg gcagacatcg gcggcatcat tttgagtttt ttcagttcgg acaggcattt
    12601 ttcttccgct tctttactca tacccgcctt tttgatgcct gcttccaagg catccagttc
    12661 gccgttttcg tcttcttcgc ccaattcttt gtgtatcgct ttaatctgtt cgttcagata
    12721 atattcgcgc tgggattttt ccatttggcg tttgacgcgt ccgcgtatgc gtttttcggc
    12781 ctgcataatg tcgagttcgg attccagctg tgccagcagg aattccatcc gtttgccgat
    12841 ttcggaaatc tccaaaatct gttggcgttg cgtcagtttc aactgcaaat gcgctgcgac
    12901 tgtatcggtt agccggctgt tttcggcaat gccgttaatg ctgccgataa tttcggcggg
    12961 gattttttta ttgagtttgg cgtattgttc aaactgcgcc aacagggtgc ggcgcacggc
    13021 ttcgaggtcg gtattgccgc ccgtgtcttc ttccacgacc gcctctatat gggaaacgaa
    13081 cagcccgccc gtgtcttcaa tagtcagaac acgtccgcga tacagccctt cgaccaatac
    13141 ttttaccgtg ccgtcgggta gtttcaacac ttgcaggact tgtgcgaccg taccggtctg
    13201 atacaggtcg gcggcaatcg gttcttctac cgccgcatcg gtttgcgcca acaggaaaac
    13261 cggctcctcg cgggtaatgg cgttttccag tgcggcgatg gatttcggtc tgccgacaaa
    13321 cagcggcaga accatatgcg ggtaaacgac gacatcccgc aaaggaaggg ttgccaaggc
    13381 ggcatattcc tcaaaatgct tttctttttg tgtcataggt actctcttgt gtctgacaga
    13441 ttgccgattt tcgcgtacat tggggttgaa ggtattattt caagcatatg tggtttattt
    13501 atggagtttg atgcgatgcc gtctgaaaca ttccggcttc agacggcatg ggcttggaaa
    13561 gacaaggcag gaacaaaaaa ctgttctgtg ttgccgctcc ttgctgtacc atccgtatgg
    13621 tttacggttc tgccgcccta ttttcaaaac atgacgcagg tatcccatgt cttcttttat
    13681 ttttcccgat aacgggttcg aatggcgcaa cgaaagtctg caaaagccgc ccaaaggttg
    13741 gcattatgtc cgcgaaagcg gtgcaaacgg cattttgaag gctgcctatc aaaatattgc
    13801 aactgtctgg cagggcgatt tccacaatgc caaacaggtg ctttccgcaa tgaagaagag
    13861 ggttcgcaaa cctgccgccg tccgttccga tgcggatatc gccgcccttt tccatgccca
    13921 ccgtatgaag caggcgcagc agagccgtat tctgaatatg cttgccgttg aaatccgccc
    13981 cggttttgtg ttggacaaca aacgcgcgcc cgatatacgc gccgccctgc tcgacgtgta
    14041 cggagaggcg gacggcaaac cgtttttcct gccgctcaat ctgctgctgg ggtttatggg
    14101 tgcgcacgag tggcataaga aaggggttgc cgttccgcag ctgggcggca gcatacacgt
    14161 tcctttcggc gtattctcgc cgttgcgcgg cgaatacctc gacctgctcg cccatgcgcc
    14221 gtcaacgggt tttcagacgg cattagatat cgggacgggc tcgggcgtgc ttgccgccat
    14281 tttggcgaaa cagggcattc cttccgtcat cggcacggat accaatccga aagccgtcgc
    14341 ctgcgcccgt gccaatattg cccgtttggg ctttgaaaaa caggttgaaa tccgcgaaac
    14401 cgatctgttt cccgaagggt ttgtcgatct gatcgtctgc aatccgccct ggcttcccgc
    14461 caagccgact tccgccgtcg aatccgcgtt atacgacccc gaatctgcga tgctggctgc
    14521 gtttttgcgg gatgcgccga aacatctgaa tcccgacgga gaaatccgcc tgattatttc
    14581 cgaccttgca gagcatttgg ggctgcgccc tgccgatttt ttggaaaagg catttatccg
    14641 ggcgggtttg cgtgttgccg atatgataaa aaccaagccg aagcacaaaa aagccgcgaa
    14701 tccgagcgat ccgcttgctt ttgcgcgaac ccgggaaacc actttcctat accgtttgaa
    14761 aaaggcataa ggggcggcgg cgcggcattc gggcggatta ttcttgtgaa aatacccgct
    14821 cgagcatact gcccaacgcc gtctgacgcg ttttgacggt gacggcagct tgccggaagg
    14881 cttcttcccc gccgaagaat acgaacttct ctctcgcgcg ggtaatggct gtgtaaagca
    14941 gccctctgct caagcccgaa agcgcgtcgt ccccttcgtc cgaaggtgcg gcggaaggcg
    15001 gcagcagcca gacttcccga tattccgaac cttggctttt gtggacggtc atggcgaatg
    15061 cgggttcgaa atcgggcagg cagcttaccg ctaccttttt aaatccgtcc gcatcggcaa
    15121 aataggcggc aaggccgccc tgccgtccga catcttccat aatcaatccg atgtcgccgt
    15181 tgaacagttc aagcgcgtag tcgttctgtc tgatcataat cggttctccg gcgaaatagg
    15241 cctgatgttc cggtatgttc attttgcggc gtacatggcg gcaataggct tcgttgaagt
    15301 cttccgcatc ctgccgccac gccgcgagga cgacgatatc cgagatgcct gcgtatgcgg
    15361 cttcgatatt gccgtctttt accgcctgcc aataggcttt gtgcgcccgg tacaaccttt
    15421 cgactcgagc gttcggactg cattccgaat gttccagttc gtccggaaac cggtcaaaca
    15481 atgcccacgc cccttcatcg cccgatacgg cggcacgggc aaggcagccg atgccgctgt
    15541 tgtcgccgaa gcggtggctg aacgacagat gggcggtgtt ttgcgccaac acgggtggat
    15601 ttgcgctgac gctgaaaccg tgttccggaa ggaagccggc cagcctttgg tgcgtttctc
    15661 cgtccaaaac ggttttttgt gacaaaacgg acagcaccgc ccctattccg acggacggga
    15721 gctggttttc atcccccagc agaatcacgc gcgcgccggt tttgaccgct tttaaaagtt
    15781 gcagcatcaa tgccgtatcc agcatagagg cttcatcgat aaccaatacg tcaaacggca
    15841 gcgggtaaac agggttgaac gccgcctgca ttttgggcgg gcgcagcttc agcagtcggt
    15901 ggacggtttg cccttccagt ttgagcaaat ggcggcggat ggcctccggc gcgtcaaaac
    15961 cgttgattgc acggtgcagc gcgcgcgcca tatgtgccgc cgctttgcct gtcggtgcgg
    16021 caagcgcgat gttgggaaga ttttcgtctt caccgcaaat cagcgccagc agtttggcaa
    16081 ccgttgtcgt tttgcccgtt cctggcccgc cggtaatcac cataaaagac tgcaacagtg
    16141 ccaaggcggc ggcatcgcgc tgcccttcgc tgcccgtgcc ttgaaaccat tttgcgaggt
    16201 tttgccgcgc gcctgccgcg tcgggggcgg acgtgccggc tgccgccaag cgttttatct
    16261 cggcagccaa atcgtattcc aactgccaca tcctgcccaa aaacagcctt ctgccttcca
    16321 aaatcaaagg cgcggcggat gttccgacca cgggtgcgag tgccgacagc gcgtcagcct
    16381 cgccaccgct caaacggata aacgaatgac cgttttgcaa tgcctgaaac aggcgttcgg
    16441 tgcagtttgc aagcacttcg ctgcctgaac ccgcatagcg tgccaaaaac cagattgccg
    16501 cccttgccgc cgcttgggca aattcatctg tctgccgttc cattgtattc cttatccaaa
    16561 tgccgtctga aggcgtgggg cttcagacgg cgcggtgttg tttcggctgt ttaggcgttt
    16621 gcgccctgtt tggggtgcag gctgccgtct ttcatgacca tcacgcgctc gaagcggccg
    16681 gcgagttcgt cgtcgtgcgt tacgaccacc agccccgttc ccaattctgt tttcagttcc
    16741 agcatcatat ccaaaacatt cctggcgttc gcacgatcga gattgccggt cggttcgtcg
    16801 gcaagcaggc atttgggttg cgtaaccaag gcgcgtgcaa tggcggcacg ctgccgctca
    16861 ccgccggaca gttcgcccgc gcggtgcgcc gaacggtgct tcaatccgac cttttccagc
    16921 atcgccattg ccgtttctgc cgcctcttcc cgttttttct tgccgatcag aagcggcatc
    16981 atcacgtttt ccagtgccga aaattcaggc agaagatggt ggaactggta cacgaaaccg
    17041 agatggcggt tgcgcaggat gcccagttgt cgctggctta atgtacgcaa atcctcgccc
    17101 atcagcagca ccctgccttc agacggcata tccagcccgc ccaaaatatg cagcagcgtc
    17161 gatttgccgc tgcccgaaga accgatgatg ccggtacttt cccccgcgag gatttccaaa
    17221 tccaagccgt gcagcacccg aacgtccaaa ccgccgtcac ggtagcgttt gcccacgcct
    17281 tcgcatttca aaatcaactc actcataacg caaagcctcc gccggttggg tttttgacgc
    17341 gcgccagctc gggtagagcg tcgccacgaa agacagtccc aaagaaatgc aggcaatcag
    17401 ggcaacgtcg cccatatcga catcgctggg caggtagtcg ataaaataaa cctgcgaatt
    17461 gataaggtgg acaccgagca ggttttcaaa aaacgccacg actctgccga cgttccaacc
    17521 caaaagcacg ccgcagacca cacccgccag cgtgccgaaa aagcctgaaa acgcgccctg
    17581 caccataaaa atcttcatca cgccggcagg ggaaagaccc aaagtccgta aaatcgcaat
    17641 gtccgcctgc ttttccgtaa ccgccatcac caaggaagag acaaggttga acgccgccac
    17701 ggcgataatc agcgtcagga tgatgaacat catccgtttt tccaactcga ccgcctcaaa
    17761 atagcttcga ttgctgaacg tccaatcgcg cacccaaacc gtatcccttt gcgcctccgg
    17821 aatcagcttt gccgtcaagg cgggggcgtt ttgcgggtcg gcgagtttca gccgcagccc
    17881 cgcaacttcc ttatccaagc ggtacagtac gcgcgcgtct tggatatgcg tcatcgccaa
    17941 ggagttgtcc acttcgtaaa cacccgtctt aaccagaccg accacggtaa actgtttcaa
    18001 cctcggtacg actccggcgg gcgtaacatt gccctccggc gtgatgacgg taactttatt
    18061 gccgacttcc gcccccaaag cctccgccaa gccgacaccg aggataatgt caaactcgcc
    18121 cggaatcagg tcttcaaatt tgcctgccgg cattttgtcg ccgtattcca ccactttgcg
    18181 ctcttcagac ggcaaaatgc cgcgcatctg cacgcccctg atttcgcccg cattggccag
    18241 caatgcctga ttggaaacat agggcgcggc agccaaaata cctttgcggt tttcggtaaa
    18301 tcgaagcagg ttgcgccaat ccgtatccgt attgtcgata tagccgattt cggcgtgcgg
    18361 cgcgacattc aggagctgcc cgcgtatttc tttctgaaag ccgttcataa ccgacaagac
    18421 gacaatcagc gcggttacgc ccaaggcgat tccggcaatc gaaaccatcg tgataaacga
    18481 cataaagccg ttgcgctttt tcgccctgag atacctcaag cctatccaag cctctagaga
    18541 aaacataacg ctaccttaaa aatgtctgca aacgtgccgc cccggacggc ggtttggggg
    18601 cggcggcaaa agtttattgt accgtaaaac ccaggcagcg tccgaacgcc cgagtgcggg
    18661 ggacggggcg gcagagcggg cggaaaactt gcacaacgcg tcaaactgcc ctatcctttc
    18721 cttcagaaaa accgtttctt gaggaaaaca atgaatatcc gaactgcttt tgctttgtgc
    18781 gccatcgcct tatccgccgc tgcggctgcc tacgccaaag aaatcaaaat cgatgccaac
    18841 aacacgcctt attccgaagc cgacgcgcaa aagctggcgg caacggcagt cggtatgggt
    18901 gttaaggaac ctatcagcct gaacggcggc agcggcagca ttaccgtgtc cggcagcagc
    18961 gcgacgcagt gcgtgttcaa agtcggcaac ggaggcgcat tgcagattca agggctaaac
    19021 tgcaagtaaa ccgcccggaa aaatgccgtc tgaaggcttc agacggcatt ttgcattggc
    19081 ggcgttatgc cccgccttct ttaatcaggc ggcgttcgta caccgcctgc gccagcgttc
    19141 ccgcatcgac atattccaat tcgccgccca aggggatgcc ctgcgacagc ctgctgactt
    19201 tgtaaggcag gtttttaaaa aactcggaca ggacatacgc cgtcgcattg ccttctgcgg
    19261 taaaagcggt tgcaataatg atttcttcga cttccccgcc gcccagccgt tgcgccagcc
    19321 tgtccaatgc gatggcggat acgtccattc ccaatgccgt attgatttgc cccatcagga
    19381 cgaaatacag cccgtcgtgg cagtttgcca cttccatatt cgacacgtcg gcaggcatat
    19441 gcaccaccat cagccgccgc ccgtcgcgtg tttcatcggc acaaatatcg cacaatccgc
    19501 cttcgcaaaa cgtgttgcac atcgcgcaat ggtgaacctg cttcaatgcc gtctgcaagg
    19561 catccaccag tttttcagcc tctttgcgct tgtgttggag caaatgatac gctatccgct
    19621 gtgccgattt cggcccgacg ttgggtaaaa ccttcagcgc gtcgatcaat ccttggaagg
    19681 catcttgttt tttgtggctc atcatattcc gccgtatggg aaaacggccg gaatattccg
    19741 accgttattt tgtcaacaaa agtgtcaatt actgaccgcc gccgttgtcg accgattgcg
    19801 ctcctttggt ctgtttgatt ttgccgttga aataacgtat caacaagtcg aaggtattgg
    19861 cagactgctg ctgcgccaaa gcctgttttg caagcggaag ctgtgcggcg atatcatccg
    19921 gcggggttat agcctgtact tcgacaatca cgggtgccgg cagaccggtc agcctgacgt
    19981 aggcgggttt gccgtttgtc ggttttgctt tcagcagttc cgcataagcc tcgggcggca
    20041 tggactgcct tgcctgctgc gcgcccaaaa cggacacttc cgaccatttc acatcaactg
    20101 ccttgccgcc gttcagttgg gtaagcacgt cttttgcctt gttttcggca agtttggcgg
    20161 cttcggcacg gatataagcc tgacgcaccg catctttggc ttcggcaaac ggcagggttt
    20221 tctcttcgcg gacttctttg gcgcggacga cccacgcgat ttcattattg atagacaaca
    20281 cttcggaatt gtgttttttc ttcaatacgt cgtcgctgaa tatggcattg atcaggtttt
    20341 caggcagtcc ggacatttgc gcgtcctgcc tgctcagcca agtttcttgg gtttcgactt
    20401 tcaaactgct gtttttggcg gcttcggcaa gcgaagcggg atggttgaac gcgtcatcgc
    20461 tcagcttttc ttttgcctta ttgaagtcgg caaccgcctt tttcattttc aattcgtttt
    20521 cgacggcggc tttttcctgc tcgaaagaaa gtttggcttc atttgccggc aaacgcgcca
    20581 cgcgctcttc aaacgcattt ttcacttccg tttcactgac ggtttgcttg tctgcaaaat
    20641 ccttcagatt caaggcgaca tattccaatt tgaccgcctg cggcagcaga tagtcttttt
    20701 tgttcgcatt ataaaatttc tgcaaatcgg cttcagacac tttgacttgg gcgatgaact
    20761 cgtcggggtt gaaagtgtgc gaacggatgg tgcggttgac ctgcgtcagc ctgatcagct
    20821 gttccgcctg cgcgtcgccg accaatacgc cgttttggac gaggtttacc aaattctgca
    20881 aggcaaactg atcgcggatt tcttcgacaa actggtcttc agacatatgg cgttgggaaa
    20941 ggtagcggtt taaaagcgcg tggtcgaatt tgccgtttgc gtcgtggaaa ttgggatcgt
    21001 ccacgataat ttgcttgatt tgttcggaag aaaccgaaat gcctaacagc ttcgcgccct
    21061 gtttcaggta ggcgcgttgc agcagggatt ggaacaccgc gtcgcgcgaa gggccgccgc
    21121 cgtccgcctg ttcgttctgt atggcgttgt tgatggagtg gtcgctgatt ttttcgtcgc
    21181 ccacttggac gatgtagtcg gcacccggat gggataccgt gctgaccccg aagccgacga
    21241 aggttaatgc aatcaggccc aaaaggactt gggcgggcgt tctgtatttt tcgatggaat
    21301 ggaacatatt ttaaatcggg atatagaatg ggaaattcaa gtcgggtatt gtaacggttt
    21361 ttatccctgt ctgcacgggg cttgccggtt gaagatgccg tcgtaggttt cttcgctgaa
    21421 gccggcgtaa accttgccgc cgcactccaa tacgggacgc ttgatcaggc tcggcatttc
    21481 ggacatcagt ttgacggcct ccgctgtcga ggacagggct ttttgctgtg tttcggcatc
    21541 gagtttgcgc cagcttgtcc cgcgtttgtt gagcagggtt tccaaaggca cttgttccag
    21601 ccacgagcag atttccgctt cagacggcat ctgttttttg aaatcccgaa actcaaactc
    21661 caagccgtat ccggcaagtc ggtttttggc ttttttgacc gtatcgcaat ttgggatgcc
    21721 gtgaaggact atcatttgga aaccttttgt ctgaaataat aaaacggata ttttactata
    21781 agtgtctgaa aatttgcccg tctgtttcag acggcggggc ggttatgtta caatccgaaa
    21841 attcgaaaaa tttaatctct tgttcaataa aggctttacc aatcatgatt tctaccaacg
    21901 gcatcaccat gcagttcggc gcgaagccgc tatttgaaaa cgtatccgtc aaattcggcg
    21961 aaggcagccg ctacggtttg atcggcgcca acggctcagg caaatccacc ttcatgaaaa
    22021 tcctcggcgg cgatttggaa cagaccgccg gtgaagtcgc aattgaaaac ggcgtgcgtt
    22081 tgggtaaatt gcgccaagac cagtttgcct acgaagacat gcgcgtgctg gacgtggtga
    22141 tgatggggca taccgaaatg tgggcggcga tgaccgaacg tgatgcgatt tacgccaatc
    22201 ccgaagccac cgaagacgac tacatgaaag ccgccgaact ggaagccaag ttcgccgaat
    22261 acgacggcta caccgccgaa gcgcgtgccg ccgaactgtt gagcggcgtg ggcatttccg
    22321 aagatttgca caatgcgaaa atggcggaag tcgccccggg cttcaaactg cgcgtattgc
    22381 tggcgcaagc cctgttctcc aagccggatg tgctgttgct tgacgaaccg accaataact
    22441 tggacatcaa caccatccgc tggctggaag gcgtgttgaa ccaatacgac tccaccatga
    22501 tcatcatctc gcacgaccgc cactttttga acgaagtctg cacccacatg gcggatttgg
    22561 actacaacac catcaccatc tatcctggca actacgacga ctacatgctc gcttccgccc
    22621 aatcgcgtga gcgcgccctg aaagacaacg ccaaagccaa agagaaactg caagagctgc
    22681 aagagttcgt cgcacgcttc tctgccaaca aatccaaagc ccgtcaggca accagccgtc
    22741 tgaaacaggc cgacaaaatc aaatcggaga tggtcgaagt caaaccttcc acccgtcaaa
    22801 acccgtatat ccgttttgaa gccgatgaaa aagccaagct gcaccgtcag gctgtggaag
    22861 ttgaaaaact ggcgaaacgc tttgaaaccc agttgtttaa aaacctgaac ttcatccttg
    22921 aagcgggaca acgcctcgcc atcatcggcc cgaacggcgc gggcaaatcc accctgctga
    22981 aactcttggc cggcgcgtac aaccccgaat attcagacgg cctgttaccg gacgaaggca
    23041 gcatcaaatg ggcggaaaaa gccagtgtcg gctactatcc gcaagaccat gaaaacgact
    23101 tcgacgtcga tatggatttg agcgaatgga tgcgccaatg ggggcaggaa ggcgacgacg
    23161 aacaagtcat ccgcggcact ttggggcgtt tgctcttcgg cagtaacgat gtcgtgaaaa
    23221 aagtgaaggt tctctccggt ggtgaaaaag gccgtatgct ttacggcaaa ctgttgctgt
    23281 tgaaacccaa tgtcttagtc atggatgaac cgaccaacca catggacatg gaaagcatcg
    23341 aatccttgaa catggcgctg gaaaaataca acggcacgct gattttcgtc tcgcatgacc
    23401 gtcagtttgt atcttcattg gctacccaaa tcatcgaatt ggacggtaaa ggcggatatg
    23461 aacactactt gggcgattac gaaagttact tggagaaaaa aggcgtagca taagcgcaca
    23521 aaataccctc cggttgaaac aatgccgtct gaagccgctt cagacggcat tgttgataac
    23581 tttaaaatag gaagcatatg cagacttacc tcgtcggcgg cgccgtccgc gattatcttt
    23641 tgggcttgcc cgtcaaagac cgcgattggg tggtcgtcgg cgcagacgca caaaccatgc
    23701 tggcgcaagg cttccagcca gtcggcaaag actttcccgt gtttctccat cccgaaacac
    23761 acgaagaata cgccctcgcc cgcaccgagc gcaaaaccgc caaaggttac gccggcttca
    23821 gtttccacac cgacaaagac gttacgctgg agcaggattt gatgcgccgc gacctgacca
    23881 tcaacgcgat ggcgcaagat gcggacggca agattatcga ccctttcggc ggacaacggg
    23941 atttggcggc aggcattttg cgccacgttt ccccggcctt cgccgaagac cccgtccgca
    24001 tcctgcgtac tgcccgcttt gccgcgcgtt acaagtttga aatcgccgaa gaaaccataa
    24061 agctgatgcg gcagatggtg gaaaacggcg aagcggacgc attggttgcc gaacgcgtct
    24121 ggcaggagtt tgcgaaaggt ttgatggaaa aaaatccgcg caaaatgatt gaagtgttgc
    24181 gcgaatgcgg cgcgctcaaa gtcttgctgc ccgaagtcaa tgccctcttc ggcgtgccgc
    24241 aacgcgccga ctaccatccc gaaatcgaca gcggcatcca taccctgatg acgctgcaac
    24301 gcgccgccga tatgggtttg agcctgcccg aacgctatgc cgccctgctg cacgacttgg
    24361 gcaaagccaa aacaccgtcc gacatcctgc cgcgccacca cggacacgac ctcgccggtg
    24421 tcgaacccgt gcgcgaagtc aatcagcggc tgcgtgcgcc gaaacattgc gccgagcttg
    24481 ccgaattggt ttgccgttgg cacattattt tccaccaagt cggacagctt aaaagccaaa
    24541 ccattctgaa cgtattgaaa aaaaccgatg ccttccgccg tcccgaacgc tttcagacgg
    24601 cattgaacgt ctgcattgcc gacacgcaag gccgtctgaa ccgcgaacac acgccctacc
    24661 cgcaacgcgc acactggctc gccctgctcg aagccgccaa tcaggcggat tcgggcaaaa
    24721 tcgccgccga atgctgggcg cagggaaaag cgcaccttat cgccgaacaa atcgaccggg
    24781 cgcggctggc gcaaatcgcc ccgctgcaaa aagcgtttcg agcggcgcaa gacaaaacag
    24841 aaaaacatta aaacgtccaa tgcagccact tttatagtgg attaacaaaa atcaggacaa
    24901 ggcgacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacac tgtactggtt
    24961 tttgttaatc cactataaag ttttgaggac gatacccaat ccaagctttg caacagccgc
    25021 cgccatatcc gctataattc acgcttcagc cattccgccc ccgacataaa atcatgaccc
    25081 tgaaaaccga tttattgctt aaaatcaaca acgaagatta tcaacgcctc atcctcaaac
    25141 acatctcgga attcagcgag ggcgaaatcc gcctgttgaa cgaaatcctt gaaaaattca
    25201 atttcgacgt cgttcaggcg caggcattgg cgcaggcggt aatgcagcaa gtccgcttcg
    25261 accccaacgc ctaccacatc gacagcgacg acgaagacac cacaggcatc tgcccacact
    25321 gcatcaaccc gcctatgccg cccctgcgcg actatctcgt ttggcgcgaa acgcgcgggt
    25381 aaaacgcttt tgaccgttat cttttcaatg ccgtctgaaa cgccgccgac cgttcagacg
    25441 gcatacccga caaagggaac actatgctgc aaaccgacaa cctgactgcc gctcaaccgc
    25501 aacgcattgt tgccgcccaa accgcctccg cacaggaaga actgctcgaa cgcgccctcc
    25561 gccccaaaac gctggacgac tatatcgggc agcataaagc caaagaacag ctcgccattt
    25621 tcatccaagc cgccaaaaag cgcggcgaag cactcgacca cgtcttgctt ttcggtccgc
    25681 ccgggctggg taaaaccaca ctggcgcaca tcatcgccaa agaattgggc gtaaatttgc
    25741 gccaaaccag cggccccgtc ctcgaacgcg ccggcgacct cgccgccctt ttgaccaacc
    25801 ttgatccgca cgacgtattg tttatcgacg aaatccaccg cctcagccct gttgtcgaag
    25861 aaatcctcta ccccgcgctt gaagactacc ggctcgacat tatgataggc gaagggcccg
    25921 ccgcccgttc cgttaaaatc gacctgccgc ccttcacgct catcggcgcg accacccgcg
    25981 ccggtatgct gaccaatccg ttgcgcgacc gcttcggcat cgtctcccgc cttgagtttt
    26041 acgaaaaccg agaccttacc accatcgtca gccgttcggc acaactgttg cagctcgata
    26101 tgtccgaaga aggcgcggaa gaaatcgcca aacgcagccg cggtacgccg cgcatcgcca
    26161 accgcctgtt gcgacgcgtg cgcgatttcg ccgacgtgaa aaacaacggc acaatcgacg
    26221 gcggcatcgc cgatgccgct ttaagtatgc tggacgtgga cgcgcagggg ctggacgtga
    26281 tggacaggaa atttctcgaa gccgttttgc acaaattcgg cggcggcccg gtcggtttgg
    26341 acaatgttgc cgccgccatc ggcgaatcta cagacaccat cgaagacgtt atcgaaccct
    26401 accttatcca acaaggcttc ctgcaacgca ccccgcgcgg caggatggcg accgaacgcg
    26461 cctacctgca tttcgggctg cccgtcgaaa aataacgcaa tgccgtctga aacagagcta
    26521 attttcagac ggcatttcta tttcaatcat tggcgcaagg ttcagcctgc cgcttttttc
    26581 cagttccgcc ctcatcgcat caatcaccgc cttatagtct ggtttgccga aaatcgcaga
    26641 accggcaaca aaggtatccg caccagctcg ggcaacggcg gcaatattgt cggttttgat
    26701 gccgccgtcc acttcgatgg cgatgtgccg cccgctttgt gcttcgtacc gatccagcat
    26761 cgcccgcacc cggcggattt tttcaagggt gtgcgggatg aagctttgtc cgccgaatcc
    26821 ggggttgacc gacatcagca aaaccatatc cagcctgtcc aatacgtttt ccaacagata
    26881 tacgggcgtt gccggattca acaccagccc cgcctgacag cccatatcac gaatcaggct
    26941 caagctgcgg tcgatatggc ggctcgcttc gggatgaaaa gtgataatcg atgctcctgc
    27001 tttggcaaac gactgaatca ggtcgtcaac gggttcgacc atcaaatgca catcgatcgg
    27061 cacgcttgca taaggcttca acgccgcgca aaccataggg ccgaaggtca ggttcggcac
    27121 ataatggttg tccatcacgt caaaatggat cagatctgca cctgccgcaa tgacgctttc
    27181 cacctcttct ccgaggcggg caaagtctgc cgacaaaata ctgggtgcga tacggtaggc
    27241 ggtcatattt tttccttcaa tgcctttacg ggcggactca acgcgggaaa gccccgaaac
    27301 agcattatta acaaaagtta actgccataa taccatcttt cacacggcaa tcaagtatat
    27361 tgtccgaaat atctggatta aaggaaaagc cgtgccactg cctgctccct gccgttttgc
    27421 caaacctgcc gcctcttttt taagtatggc tttgctttcc tgtcagcttt cccacgccgc
    27481 cacggcttat atccccccga acgattttca accgaactgc gacatacgcc gactcgggct
    27541 gacccaaagt cagcacaatg agctgcgtaa aatccgcacc gccttcaaaa tggcgggcga
    27601 cagggcgcgt ttgaaggtta tgcattccga acacagccgc cgccggtctg tcgtcgaaat
    27661 catttcctcg gatgttttta atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt
    27721 gtccggtatg gattttgcgg tggacgaatt ggaaatccaa caccggttct tccatatcct
    27781 cacaccgcaa cagcagcaaa tgtggctttc ttcctgcctc aaataatccc cgaaacgctc
    27841 acaacgcccg ttgtttcggc agcctgcccg cccagtcgca ggcaaactgc cacgcggaac
    27901 gtcccgaacg gctgccccgc gtctgcgccc actgcaatgc cgccatctgt gcggtttcat
    27961 catacggcac gccgaaatct tccagccaat tttgcaccgc cgccagataa tcgttttgat
    28021 cgaacggata aaaactgagc cacaatccga atcggtcgga cagggatatt ttttcttcca
    28081 ccgcttcttt ctgatggatt tccccccgca tccccgtcgt accggcattc tcgtcaaaat
    28141 attcgggcat caggtgccgt ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt
    28201 gagacagacc gccgtctaac gcggttttca atgccttata ggtttcatcg ccgctttcaa
    28261 acgacaaatc gtcgcaaaat acgataaatt tttccggacg ttccttcaaa agcgtcaaca
    28321 ggtaaggcag gccgattaaa tcgcttttat cgacttcgat caggcgcaat cccttatccg
    28381 catattcgtg tagcagggct ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca
    28441 tcaatacatt gttcgcgggt ctgccgacaa tgaactgttc ggtattacgc accagcaatt
    28501 cggtttgcct gccgactccc gccagcctta ccaagggaaa ggtgtgcgga tcgggcaagt
    28561 gttccaaaaa accttttttg cccgcactct gccagcggaa ggcaagcgcg ttccaatccg
    28621 tatgcccggg ttcgggcgga agcacggcat ccaaacgccg caaaacggca taggctttat
    28681 cgagaaattc gttcaattcc atctctgcct cactttgcat atctttgcgc catcagccgt
    28741 tcgacggtat cgacgattgc ctgcgtattc ggatcgattt cgatgttgat cctgtcgccg
    28801 acctttctgc tgccgaacag cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa
    28861 cggccgtctt cgactttgcc tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg
    28921 gtcaggatat agggtttgag ttcatgcggg agcgaaaacc aaaccgtgcg gttgaacccg
    28981 tcccgttcga tttcgacaat aggcacggtt gccataatgt gtccgctcat gacgtgtccg
    29041 ccgatttcgt ctccgaaacg cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc
    29101 agccccaaat tggtttttgc caaagtttcc gccattaaat cgaaactgac gcggtttcct
    29161 tcgatttcgg taatcgtcag gcagcagccg ttattggcga ccgatgcgcc gcgttgcaga
    29221 ttgtccgccg cctcttgcgg aagctcgacg acataagtgt gaaatgcctc cgacgggcgg
    29281 tggattgccg tcagttttcc caatccttga acaatgcctg taaacataat cctgtttccc
    29341 tgtgtcggta aaaatggtgc aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg
    29401 ccttcagaca gcattatgcc tccgattcgg gcaaaaaccg cccggtatgg cttgaccttt
    29461 cctttccacg ccggtcggcg gtcttgccct tatccctcct gcaaatcgat ttgcgtgttc
    29521 aagtcggcaa aatgcccgtc aaactcgaat ctgaccgacc gcgccctttg ctgctgcaac
    29581 cagcttctta atgttttcat acccgaaaac agatagggaa tcgcgctttg cagaatctgc
    29641 gggcggatat acataatgtt gtagtgcatc gttatcggcg tttccacata atacgcattg
    29701 cacaacggtg tgcgtttcga cacggtttca aacctcgcca ccaaatcgcc cggcagatac
    29761 ggcatatcgc acggcacaac caaaagccag tcggcagtcg ccaactgcaa atcgttggct
    29821 gcggtacaca atgccgaaag cgggccgaaa tgctgccact gccgtgcgtc cggaaaaata
    29881 tgcggacttc ttcgagcata ttcttccaaa ttccggttgg tgctgatggc gatatggctg
    29941 acctgcggcc tgaccctgtc gatgacatga tctatcagtg ccttaccccc caaaagagca
    30001 agccccttgt cctcgcctcc catacggctc gcctgaccgt cggccagtat cagggcaaaa
    30061 gttttcattg cggatgttct cttggaaaag ttcgaggttt tcatgattgc agtctgccgt
    30121 tcccaatgac tcaaaatgcc gtctgaagca gacggcaaat aaattcatat tatctgaatt
    30181 ttatcataac atgatttaac actgaagcgg tacggattat gttttcctta accaattctt
    30241 tctgagcagt tgtatctaat cccaaagaat gatattgttt gcattattta gaacaatttt
    30301 tcgccgagca tgatactgcc agcccgtttt tcagacggca tcagcctttc cctacgcctg
    30361 aaactcctga ccggagtgtg ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg
    30421 ctctctttgc gtctggaaaa cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg
    30481 caggcatacc gtctggcata catggcgggt gaaggatctc cccgctccca aatagacaat
    30541 caggttgacg aatttgaaaa aagcctgaat cgtatttcac aaagtgatgc catccatccg
    30601 ctgattcctt cggacacccc tcttgcttat gatttgatac aatccatgct gattatagat
    30661 tggcaggcac acatcctccc cccgctccag tcctaccggc gaccgactca ggtcgatctc
    30721 taccgctttg ccggaaacat cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa
    30781 aacacatggt ggctcaggcg ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct
    30841 gtactgatgc tgttttggca ccagatttgg gttatccggc cgctgcaggc gttaagggaa
    30901 ggtgcggaac gcatcggacg gaggtgtttc gatattccgg ttcccgaagg cggtacgccg
    30961 gaattcaaac aggtcgggcg ttgtttcaat caaatgggcg gcaggttgaa aattttatat
    31021 gatgatttgg aaggacaagt cgccgagcag acacgcagtc tcaaaaaaca aaatcaaaac
    31081 ctgaccctgc tgtaccaaac tacacgggac ctgcaccaat cctacatacc gcaacaggct
    31141 gcagaacatt ttctaaaccg tatcctgccc gccgtaggag cagattccgg cagagtttgt
    31201 ttggacggcg gatccgatgt ttatgtttcc attcatcatg cggattgcgg cacagcagct
    31261 tcggatttgg ggaagtacca tgaggaaatc ttccccattg agtaccagaa cgaaacattg
    31321 ggcaggctgt tgctcagctt tccaaacggc atttctcttg atgaagacga ccgcatcctg
    31381 cttcaaacac taggcaggca attgggcgta tcgcttgccg gcgcaaaaca ggaggaagaa
    31441 aaacgcctgc ttgcagtatt gcaggaacgc aacctgattg cgcaaggatt acatgacagc
    31501 atcgcacaag cattaacgtt cctaaaccta caggtacaga tgctggaaac cgcctttgcc
    31561 gaaaacaaac gggaggaagc cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa
    31621 tgttatgaag atgtccgcga actgctgctc aacttccgta ccaaaatcag caataaagaa
    31681 tttcccgaag ccgttgccga cctattcgcc cgctttacgc aacaaaccgg gataacggtc
    31741 gaaaccgcct gggaaaacgg ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt
    31801 tttatcctgc aggaaagcct gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa
    31861 ttcacccttt ccgaacacgg cggacgcttt accatgacca tccaagacaa cggacaaggt
    31921 ttcgacacgg agaaaatagg agaacccacg ggcagccatg tcggactgca catcatgcag
    31981 gagcgtgcca aacgcatcca tgccgtttta gaaatccgtt cccaagctca acagggaacc
    32041 accgtctcat tgacggttgc atctgaagaa agcttgaaat gaccattaaa attatcctga
    32101 tagacgacca taccctcttc cgcagcggca ttaaatccct tttgtcgcgc caacatgatt
    32161 ttgaggtcat cggcgaagcc gcagacggcc tctcgggcat taaaatgatc aaccggctcc
    32221 aacccaatgt cgtcctgctt gaccttgata tgcccggtat gaacggacgc gaagcactct
    32281 cccaaatcat cagcatcaat ccgcagcagg cagtgattat gctgaccgtt tccgaagaca
    32341 acgacgactt gaccgaatgt atgcgcatcg gcgcgcgcgg ctacctgctg aaaaacatca
    32401 acgccgactt tctgctcgaa agcatacgca aagccgccga aggcgataat gtattctcgc
    32461 ccgagatgac cgccaaactc gttaaaagcc tgatttctcc ccagtccaca caagggacgc
    32521 aggcactctc ctcacttacc cctcgtgaac tggaaatctt gggctatctc gccgcaggac
    32581 acagcaacaa aatcatcgcc cgccacctcg atcttgccga atccaccgtc aaagtccacg
    32641 ttcaaaacct gctccgcaaa ctcaacctca gcagccgggt gcaggccgcc gtttacgcca
    32701 tccggcacaa cgtcccccaa cctgtgccgg aataaggcgg gttcagacgg catattaggg
    32761 gttttaatcc ccgtacggtc attcggataa cagaccaagc atgtaagttt atgcccccat
    32821 aagtacgctt ggcatagcag taatattgtt cggtttagtg ttttccgttt gcccctatct
    32881 gatactgcaa tatcaactat gccgtctgaa aacgcatcat catgatattt tcagacggca
    32941 taataaaaag cggaaatact aatgcagggt aaaatgttcc attccgaatc ccataaatat
    33001 acaatggctt atatcgttta agcatgtgta acccaccctc atatcaatca ata
//
LOCUS       DAWGJV010000004        29725 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWGJV010000004 DAWGJV010000000
VERSION     DAWGJV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.004"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(180..827)
                     /locus_tag="WHQ94_000118"
     CDS             complement(180..827)
                     /locus_tag="WHQ94_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ2127473.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(824..1192)
                     /locus_tag="WHQ94_000119"
     CDS             complement(824..1192)
                     /locus_tag="WHQ94_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ2127474.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1202..1873)
                     /locus_tag="WHQ94_000120"
     CDS             complement(1202..1873)
                     /locus_tag="WHQ94_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ2127475.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGIFGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2038..2757)
                     /locus_tag="WHQ94_000121"
     CDS             complement(2038..2757)
                     /locus_tag="WHQ94_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2127476.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFATTIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2841..4154)
                     /locus_tag="WHQ94_000122"
     CDS             complement(2841..4154)
                     /locus_tag="WHQ94_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ2127477.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4233..5105)
                     /gene="hpnD"
                     /locus_tag="WHQ94_000123"
     CDS             complement(4233..5105)
                     /gene="hpnD"
                     /locus_tag="WHQ94_000123"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ2127478.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQKKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGEMPEHPVNQALQQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5276..7138
                     /gene="hscA"
                     /locus_tag="WHQ94_000124"
     CDS             5276..7138
                     /gene="hscA"
                     /locus_tag="WHQ94_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ2127479.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7220..7876
                     /locus_tag="WHQ94_000125"
     CDS             7220..7876
                     /locus_tag="WHQ94_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2127480.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            7912..8673
                     /locus_tag="WHQ94_000126"
     CDS             7912..8673
                     /locus_tag="WHQ94_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ2127481.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            8728..9069
                     /gene="fdx"
                     /locus_tag="WHQ94_000127"
     CDS             8728..9069
                     /gene="fdx"
                     /locus_tag="WHQ94_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ2127482.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9356..9943)
                     /locus_tag="WHQ94_000128"
     CDS             complement(9356..9943)
                     /locus_tag="WHQ94_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ2127483.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10156..10353
                     /gene="iscX"
                     /locus_tag="WHQ94_000129"
     CDS             10156..10353
                     /gene="iscX"
                     /locus_tag="WHQ94_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ2127484.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10421..10822)
                     /locus_tag="WHQ94_000130"
     CDS             complement(10421..10822)
                     /locus_tag="WHQ94_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ2127485.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            11033..11992
                     /locus_tag="WHQ94_000131"
     CDS             11033..11992
                     /locus_tag="WHQ94_000131"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ2127486.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12060..13370
                     /gene="tilS"
                     /locus_tag="WHQ94_000132"
     CDS             12060..13370
                     /gene="tilS"
                     /locus_tag="WHQ94_000132"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ2127487.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMQ
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            13444..14229
                     /locus_tag="WHQ94_000133"
     CDS             13444..14229
                     /locus_tag="WHQ94_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ2127488.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14239..14436
                     /locus_tag="WHQ94_000134"
     CDS             14239..14436
                     /locus_tag="WHQ94_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127489.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            14698..15051
                     /locus_tag="WHQ94_000135"
     CDS             14698..15051
                     /locus_tag="WHQ94_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127490.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYGQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15063..15368
                     /locus_tag="WHQ94_000136"
     CDS             15063..15368
                     /locus_tag="WHQ94_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127491.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            15430..16806
                     /gene="mpl"
                     /locus_tag="WHQ94_000137"
     CDS             15430..16806
                     /gene="mpl"
                     /locus_tag="WHQ94_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ2127492.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTVVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            17013..18065
                     /gene="bioB"
                     /locus_tag="WHQ94_000138"
     CDS             17013..18065
                     /gene="bioB"
                     /locus_tag="WHQ94_000138"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ2127493.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINQLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18143..18778)
                     /locus_tag="WHQ94_000139"
     CDS             complement(18143..18778)
                     /locus_tag="WHQ94_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ2127494.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKV
                     RKLITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19101..19361
                     /locus_tag="WHQ94_000140"
     CDS             19101..19361
                     /locus_tag="WHQ94_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ2127495.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAAGGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19424..21283)
                     /gene="ilvD"
                     /locus_tag="WHQ94_000141"
     CDS             complement(19424..21283)
                     /gene="ilvD"
                     /locus_tag="WHQ94_000141"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ2127496.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21456..23225)
                     /gene="cysI"
                     /locus_tag="WHQ94_000142"
     CDS             complement(21456..23225)
                     /gene="cysI"
                     /locus_tag="WHQ94_000142"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ2127497.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23252..25066)
                     /locus_tag="WHQ94_000143"
     CDS             complement(23252..25066)
                     /locus_tag="WHQ94_000143"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ2127498.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIPVRRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAADGWTGHIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLVNQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGIAPFRAFLQQRATENATGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKEGFLHRYDFAWSRDQEEKIYVQHKIREQAAELWQWLQEGAYVYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25063..26349)
                     /locus_tag="WHQ94_000144"
     CDS             complement(25063..26349)
                     /locus_tag="WHQ94_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ2127499.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGANEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26388..27311)
                     /gene="cysD"
                     /locus_tag="WHQ94_000145"
     CDS             complement(26388..27311)
                     /gene="cysD"
                     /locus_tag="WHQ94_000145"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ2127500.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVTDISERSATRLDDQASEVAMEKRKKEGYF"
     gene            complement(27349..28089)
                     /locus_tag="WHQ94_000146"
     CDS             complement(27349..28089)
                     /locus_tag="WHQ94_000146"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ2127501.1"
                     /translation="METTLLKPSLWQIPHIGSGSETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRF
                     QPIREDALHYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28100..29551)
                     /gene="cobA"
                     /locus_tag="WHQ94_000147"
     CDS             complement(28100..29551)
                     /gene="cobA"
                     /locus_tag="WHQ94_000147"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ2127502.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKDKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         6410 a         7825 c         8550 g         6940 t
ORIGIN      
        1 catacaacca cgccggaatt aagtttaaat ttgaataaaa ggttcgggtt ctgcaaaata
       61 cagaacccga accttgttcg gatattgaaa ccggctgccc gattttgggc ggtgcggctt
      121 gcaagtatca agattcgcat atgccgtctg aagctcggag aggttcagac ggcatatgct
      181 tatttgggct gctcttcgac gaatctcgga cctttcaaga tgccgttgtg agaatagggc
      241 gacagcaggt tgtatgcggc ggttttggaa acctgataac cgcggtcggt caggctgttg
      301 gcaatctgat tgaccactgc gctgaccaaa gcccccaaca ggccgctgtt gctgttgttg
      361 ctgccttcgc ggatgctggc cgaacccgac cacaactctt ttccgttgcg ggaatcgacc
      421 agccgtgctt tggcggatac ggtcgtcacg ctgtctaaaa tttgatatga agtgccgtat
      481 tcggtaaccg taatgtacaa aaccgcatca ttgccgaaaa tctgatgcag tttttccggc
      541 cggacggcgt gaatatcggc ggcattggtc aagccgtttt gtttgaaggt ttcctccacg
      601 actgcggcgg ggaagacgta atagccggct tcggaaagcg gagcggcggt cgaagccagt
      661 acaccccatg ttccgttgac atcgggcgat tcgttcagcg ggggaaccac caaaattgaa
      721 gccggtttgc tttccttgaa tgacgtgtag tcgaagtcgg gagctttttg aacttggcag
      781 gcagacagcg ccaacacggc ggcaagccct aaaatcaaag gtttcatcgc ttgcctcctt
      841 taccggtttt catcaggaag tccataaata cgcccgattc gggaaacagc cttttctctt
      901 cttcaaactg gcggaacgcg ccctctttgt ctcccgaacg ggaaagcagc agtcccagat
      961 gggcgtgcgc acccggggcg gcattcattt ttttgttgcc ggcttccaca aagtattttt
     1021 ccatcttttc ggtctgcttg cccaacgaag tgtcgtcgtt tttcaaacct tcatagacgg
     1081 tatcgggata gccgccgtaa taatacaggg atttttgccc gttgccgccg caggcggtca
     1141 gagccaagac cgccgcacac agcgacaaac ggctcaaggt tttcggattc atcatttctc
     1201 cttaacggtt gggttgccat gcgccgttgt caacagcctg aaccaggctg ttgacggctt
     1261 cgcggattgc caagtctaaa actttgccgt tcaaagtcgc atcgtagccg gaagtgccgc
     1321 cgaaaccgat gatttcacgg ttggaaagtg cgtattcgcc cgcgccctgt gcggaataga
     1381 cgatttcgga agtattgacg ttgacgatat tcagagccac ttttgcatag gcgatttgcg
     1441 atttgccgcg accaaaaatg ccgaagagct gatgatcgcc gacatctctg cgtccgaatt
     1501 cggttacatc gccggtaacg acataatctg cgcctttcag gttatgcgct ttgccggaaa
     1561 tgccggattc ctgttttaat gcgttcaaat tggtgcggtt cagtacgttg aagcggttgg
     1621 tctgttgcag gtgcgttacc agaatggttt ttgcctggct gcccaaacgg tcttccccgt
     1681 cggagaaaat gcctttttgg aagctggagc ggttgtcgaa tgttccgacg gaaatcgggg
     1741 tacgaacacc gtgatattgc gtattgtagg aggcgacttt tgctacctcg agactgcgtg
     1801 aggattcggt cgcacagccg gtcagtgaaa cggcagcggc ggcaaggaca acggcggtgg
     1861 aaacggtttt cataaaattt accctaaggt caagttaagg aaataacggg ttgtcattat
     1921 tgtccttatg taaatttaag tcaaggtgtt tgtctgtgcg ggacggatgc gcgcggaagg
     1981 tacggatatt tttcaaacgg aatcgcggcg cgggcgggat gcctgccttt tccggattta
     2041 gaggtaaacg atttcgccac tccgcccttt gctttcggca cttgcccacc agacaaatgc
     2101 gggcagcacg tccccgtagc ttttgcgttc gcttttggct tcgcccggat gggatttgat
     2161 gcgttgcggg gaattgatgg gtccggggac gaggacgttg gcgcgcaggt tgccgaagcg
     2221 ttcccattcg tcggcggcga ctttgcacag gtagttcaac gcggctttgg acgcgccgaa
     2281 gccgccccag taggctttgg gtgtttcgcc gtggctttcg ccgacgaaga tgacggacgc
     2341 gtcgggcgac tgcttcagca gcgggaacag ggcgcgggtc agccccatag gtgcgacggt
     2401 gttgatgcgg tattggttga cccattcggc gacggtttgg aaatccagcg gcgagagggc
     2461 gtaaaaatag ccggcgcagt ggacgatgcc gtccagtttg ccttgcgtgg cttcggcaat
     2521 ggtggtggcg aactgattga attctttttc ttccgcactc atcaggtcga aacggatggc
     2581 gaagggttcg gggtgtccgg cttcgacaat cgcgtcatag actttttcca gttttttctg
     2641 atggcgtgcc accagaatca cggttgcgcc tgccgccgca taggctttgg cgacctgttc
     2701 gcccagacct tgcgatgcgc cggtaactaa gatggttttg ttggacagtg tcgccatact
     2761 ttttcctttt tggtggtcgg tcgggggtat tttagcgttt tgccgcacct tgtaaagcgt
     2821 cccgatacca gccggcgttt tcagacggca tcgctcaggc tttgcaggca ggtttccgcc
     2881 gccgcgaaac ctgattgtac ggcggcttcg agcgtggcgg ggtagtccgg gtggaggtag
     2941 tcgccggcgg ggaagatgcg gtgccggtgc agccacgaca aatccggcgg cggggcatcg
     3001 gctgcggttg tggcgcgttt ttcggtgatg acgcgcacgg cttcgggttc gcccaaatgc
     3061 ggaaggatgc gtttgaggtc ggcgtgggct ttgtccgccc acgcccggtt tgcaaacgcg
     3121 ccgacgcggt cggaaacgct gatgacggcg gacacttcgt tttcaggcag tccgagcctg
     3181 ccccggcaaa gcagccattg caccgtgccg tcggcaaggc cggtcagcgg ggcgggcagg
     3241 cggacgggtt cggcgtagcg cagatagacg gtggtgatgg cgtggtagcg aaggttttga
     3301 tatgccgtct gaacgtgttc gggcgtgcct tcgggcagga gcgcggcggc gtggtagggc
     3361 gcggtggcgg ggacggcggc atcgaaagct tcgccgttga cgagcacttt cccgtccggg
     3421 agggtgttca gacggcatac gcgcgtttcg aggcggatgt ccgcgccgag ccgttgaaga
     3481 tccgccaagg cgggttcggc gacgattgcg cccaaatcct gtttgggtag gagatagtcg
     3541 ctgccggatt ttttcgtcag cacgccgtcg gacaaaacgt tgcacaacac gcgcaggctt
     3601 gcggtttcca aaggcgtgtt gagcgcgccc caaaccaagg gctgccaaaa ctgcatcacg
     3661 gcggcacgcg gcacgttccg ctgtttcagc cattgcgcca ctgtcgtgtc gggctgtccg
     3721 aggcgtgcgg acttctgcaa atcggacata tcggcaagca gtttggcttt gaatgcagtc
     3781 ggtgcacgcc gggcaagcag cacgccgccc aaaatatgca gcggcgcggg cagggggagg
     3841 gcgcggaact gcaaaccgcc gtgcatatgc cagtgcagcg gtacgcgcaa aaaggcggca
     3901 cggggatccg aaccgatggt tttcatcagg cgcaacacgc cccggtatgc gccgagcaaa
     3961 atgtgctgcc cgttgtccaa aaaaccgaaa ccgtcggtat ttccggccag tgtgcgcgcc
     4021 ctgccgcccg cctgccggcc ggcttcaaac agggtaacgt cggcgtgccg cgccaaggtg
     4081 acggcggcgg acagtcctgc ccagcctgcg ccgatgacgg cgattttcgg gcgcggatgc
     4141 ggcgtgttca tcatttattc ctccaatggt tgaaaccccg tttttcgggg cggcgggggt
     4201 tgggttttcc tgaagggcgg gcggtatgcg ccttatgccc gttccggcgt gccggggcgc
     4261 ggtttgaatc cgaataacca ggttttcagg gcaatgcgtt ttttgcgcgg cgaagggagg
     4321 gcgattttgt atttgaggac gttttgcgcg ccgtctcggt cgatttcgtt taaaagcgcg
     4381 taataaaccg ccgccataac cagtccgact ttttgggctt ttttgtcggc atcaggcagc
     4441 agcgatacgg cttcgcggta ggtttcacgc gcacgcttta tttggaatgc catcaattcg
     4501 gtgaaatttt ctgtcggccg gcactgcaaa atcacacttg cggggacatc aaaccgccgc
     4561 atttcctcca tcggcaggta aatccgccca ttacgggcgt cttcgccgac atcgcggatg
     4621 atgttggtca gttgcagcgc aagtcccatt ttgtcggcgt attccagtgt tttgctgtct
     4681 gaaaacccca aaatccgcgc aatcaggcag ccgactacgc ctgcgacgcg gtggcaatac
     4741 agtttcaatt cttcaaaact gccgtaacgg gcttgaacca aatccatctg catcccgtcg
     4801 attaaggctt ccagttcata tttcggcagc ttgaaggttt ccttaacctg ctgcaaggcc
     4861 tgattgacgg ggtgttccgg catctcgcca ccgaacacct tgtccaaatc gccgcgccac
     4921 cagttcaatg ttgcctgtgc aacatcgggg ttggaacatt cgtcaaccac atcgtccaat
     4981 tcgcggcaaa aagcatataa aaccgttacc gcatcccgtt ttttctgagt caggaaacgg
     5041 aagcccgata aaaaactgga gcggctttct tctgcttttt gacggcaata gtcgagtcct
     5101 ttcacgattt atattcctaa tgatgggcga gaaagacgga ttttatcggc atttggcggt
     5161 agagggcaat ttcggcggca cggcccaatc cttagcggtt tgctcaacta tcggcgcaaa
     5221 ttctgttaaa atgccgactt ccttccttta cacaccgcac cgacaggcag aatttatggc
     5281 tcttttgcag atttcagaac cgggtatgtc cgccgccccg caccgacacc gtttggcggc
     5341 aggcatcgat ttgggtacga ccaacagctt ggttgccacc gtccgcagcg gcagtgccgc
     5401 ctgcctgccc gatgccgaag ggcgcgttac cctgccttcc gtcgtccgct atctggaaaa
     5461 cggcggcatt gaagtcggca aaaccgccct gtccgcccaa aaaaccgacc cgctgaacac
     5521 cgttagctcc gccaaacgcc ttatcgggcg gacgcttgcc gatctgcatc aaaatacgca
     5581 ttacctgcct taccgtttcg gcgacaatca acgcgttatc gaactgcata cgcggcaggg
     5641 ggtgaaaacg cctgtcgaag tgtcggcgga aatcctcaaa acccttaaat cgcgcgccga
     5701 agaaaccttg ggcggcgatt tggtcggcgt ggtgattacc gtccctgcct atttcgatga
     5761 tgcccaacgc caagccacca aagatgccgc gcgtctggcg ggtttgaacg tattgcgcct
     5821 gctcaacgaa cccaccgctg ccgcaatcgc ctacgggctg gacaacgcct cggaaggcac
     5881 gtttgtcgtg tacgacttag ggggcggcac attcgacgta tccgtattgc aactgaccaa
     5941 aggactgttt gaagtcaaag ccaccggcgg caacagcgcg ttgggcggcg acgatttcga
     6001 ccaccgcctg ttctgccgcc tgctcgaaca aaacggactc tcccaactca acgaacaaga
     6061 cagccaactc ctgctctcgc tcgtccgcgc cgccaaagaa caattaacca cgcaaaccga
     6121 agcgcgcatt caggcgacgc tttcagacgg catggcaatc gacacaagca tcagtcgcgc
     6181 cgagttccac aacctgacgc agcatttggt gatgaaaacg ctcgaaccgg tcacacaggc
     6241 gttgaaagat gccggtgtcg gtaaaaacga agtcaaaggc gtgattatgg tcggcggttc
     6301 gacccgtatg ctgcacgtcc aacaggcagt cgccaccttc ttcggacaaa ccccgctgaa
     6361 caacctcaat cccgacgaag tcgtcgcgct cggcgccgcc atacaggcaa acgtcctcgc
     6421 aggcaacaaa accgacggcg aatggctgct gctggacgtt acacctttgt cgctcggttt
     6481 ggaaacctac ggcggcttgg cggaaaaaat catcccgcgc aattccacca tccccaccgc
     6541 gcgcgcgcag gactttacca ccttcaaaga cggtcaaacc gcgatgacga tacacgtcgt
     6601 gcaaggcgag cgcgagctcg tttctgactg ccgcagcctt gccaaattca ccctgcgcgg
     6661 cattccaccg atggcggcgg gtgcggcgcg tatccgcgta accttccaaa tcgatgcgga
     6721 cgggctgctg tccgtttccg cccaagaaca aagcaccggc gtacaggcgc aaatcgaagt
     6781 caaaccttcc tacggcttgg acgacggcgc gattacccga atgcttaaag acagtatgga
     6841 caacgccgcc gaagatatgg cggcacgcgc ccgcgccgaa gccgtagtcg aagccgaaag
     6901 cctgaccgat gccgtcaacg ccgccctcga gttggacagc gatttactgg atgccaaaga
     6961 attgcaacag atccggcaag gcatcgccga tttgcaaggc cgtctgaaag acggaaaagc
     7021 cgaagacatc cgctccgccg tcgccaaact cagccgcagc accgacaact tcgccgccaa
     7081 acgcatgaac cgcaatatcc aacgcgcgtt gactggtcaa agcgttgata atatttgacg
     7141 gcaaatggtt tcagacggca ttggataaag aggtcgtctg aaaaaatatc atctcacaga
     7201 aataaaataa aaaacaacaa tggaaaagtc aggcattgtc tatctgatga aaaccgtcat
     7261 caagggcgtg tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca
     7321 tttggaaaac aacggttatg cgaacgttgc cgggttggaa cgcatcctcg ccgtcaaaac
     7381 cgacaattac aaagaaaaag aaaacctgct ccatgaaatt tttagtaaaa gcaggatagg
     7441 cgataccgaa ttgttcgccg tggacgagaa tcttgtaaaa cgtttgtttt tatcgcttcg
     7501 cggcgaaatc gtgttcccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca
     7561 cgaacgcagg caggaaggga atgccggatc cggtcgcaaa caactgcttg atttggtacg
     7621 gcgcggacac cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta
     7681 caagccgaaa aaatcgaaaa tccgcctttt taaagaagca tatttacgca aaagcggtac
     7741 gaggctgacc gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga
     7801 tttggaaaaa gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg
     7861 cagaaaacca cggtaaggtg caaacagata ccgtacacgt tgaggagcag tatgatgggc
     7921 gattccgtca tttattatgt agaacaggca gacgaaccgg taaaccgtgc cggcgaacgc
     7981 gcccgtaaaa cattcaaata cttttggcgc gagctttttt gggaacgccg ccgcatcatt
     8041 cccgccttag attttgccat ggtcaaagcc ccttttttcc aagacggcga agacggcgaa
     8101 atttgcgaac atatgtggat cgacgatatt tattttgacg gtctttacat ttacggtgtg
     8161 ctgaacaata aacccggcga actgactaat gtcgaacaag gcgaaagcat ttgcgttccg
     8221 gttgacgaca tcagcgactg gatgttcgtg tgcaacggca ttccctacgg cggctttacc
     8281 atacaggcaa tgcgtggaca gatgacggaa gaggagcgta ccgaacacga tgccgcatgg
     8341 ggaatcgatt tcggcgatcc cgggcaggta ttgctggtgt atgaagaaaa agaacatccc
     8401 gaaaatctgg aagagcatcc gatgtgccgg aactgtattg acgattttcg gcaacagttg
     8461 tcccaaaatc cggattttct gcatgagcag gacgaagacg gctatacgcc gcttcatcat
     8521 gaagccatcg caggaaatgc acttatggtt caagccatgc ttgaatacgg cgcaaatcct
     8581 gcctcaaaga catcggaagg ctataccgcc ctcgattttg cccgcctgac gggctggcaa
     8641 aatgttgccg acctgctcga accgcgacat taggcagaca gttttccgaa aacgaacaca
     8701 aacacttttt acagaaagac aataaaaatg cccaaaatca ccgtacttcc acacacgaca
     8761 ttatgccccg agggtgcagt catcgataac gcacccgaag gtaaaaccgt ccttgacgtg
     8821 ctgctcgacc atgatatcga agtcgatcac gcctgcgaaa aatcctgcgc ctgcacaacc
     8881 tgccacgtga ttatccgcaa aggtttcgac agtctggaag agccgaccga attggaagaa
     8941 gacctgctcg atcaggcttg gggtttggaa gccgattcgc gcctgagttg tcaggcggtt
     9001 gtcgccggcg aagatttgat tgtggaaatc cccaaataca ccatcaacca cgcgcgcgaa
     9061 gaacactgaa aacaggccgt ctgaagccgg cacgcttcag acggcattgt tgcgcggata
     9121 aggcgcaatc gcccgaaaac aggcgttcgt acaggcggaa ctttcgattc tatagcgaat
     9181 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtacg gcaaggcgag
     9241 gcaacgctgt accggtttaa atttaattca ctatatattg atttttatcg gttttctgac
     9301 ggaacggatt ggtgcggcat ccgaggcgga ttactcgggc gcgatgcacg ggtatttatc
     9361 ggttttgcag ccggaaaaac cgccggcggg ttactttcag ggcggcggtc gggcggtatg
     9421 ccggatagcg ttccactttc gcttctatat tgattttgat tggttttctg acggaatgac
     9481 ccgatgcggc atccgggacg gcttgtgttt tttcctgccc gcctgccgga ttttcccatc
     9541 cttgcgtgaa accgaaagag acggcggcgc ggcggacaag ctcgagatag cgtccttcaa
     9601 gctccgggca ggcggcggac acgctttctg ccgtaaccgt gaagccgccg cccgacccgg
     9661 aaaggcgttc cgcaatctgc gtgtagatac gccaacgttg ggcgcgcaac attgccgcat
     9721 cgcaaccggc agccgcttta tccaaggcaa tcaggccgcg ccggttttgg tggtgaaggc
     9781 aaaccgttac tctggagagg atatgttgcc cgtccaatat ttgatacagt ttgcgtatca
     9841 gcagaatcag gcggtcaaac tcctccatct ccgacaggga atcaggatgg tcggaagcgg
     9901 tataaaccag tttggacaat tttgcggcaa acatattgtt catcaatctt ccttgtcggt
     9961 tgaaaccccg ctcttcgggg cggtagaatc agatttgttt gggagggaca actcctccca
    10021 gatcaggacg acacataggc tggtgcttga tgtgttgtcc ggcgagttga aacattcagc
    10081 catcctcaag gtgcggcagt tttgccgaaa catattctac acggcttcaa tgccggacga
    10141 taaaaggaaa ttcatatgaa atggaccgac acccggcgta tcgccgaaga actctatgac
    10201 ctgcacggcg aaaccatcga tcccagaacc gtgcgcttta cccaactgcg cgacctgatt
    10261 atggcattgc ccgaatttga tgacgatccc gcccgttgcg gcgaacgtat tctcgaagcc
    10321 gtgcagcagg catggataga cgaggcagaa taagtttcgg gaatgccgtc tgaaatacgg
    10381 cggtacgcgg ttcgtgcttc tgtttgcagt gggaacggtt ttaccagtct ccttttttca
    10441 gcctatccag ttggcggcgg tcgcgcttgg tcggtctgcc gtcgggatgg gcggaagtga
    10501 tgcggctgaa ttggtcgagc tgtttgcgct cttccctcaa tgctgccgtt tttgcgtctt
    10561 cttcatacag aagccgcgcc tcggatgccg ggcggcgttg gtggttcaaa cctttaacct
    10621 tgattttata gggaagggaa ttgagcgtca ggtcgataat atcgccgatg tctatggttt
    10681 tactgttttt gaccttcgag ccgtttactt gaaccctacc cagttcgatg tgcttttgcg
    10741 caagggaacg ggtcttgaaa aaacgtgccg cccaaagcca tttgtccagc cgcatggcgg
    10801 aagaatcgtg cttgtctttc atacgatttt gtttgaaata attgaatttg tttcgagttt
    10861 agcataagat acgccgccta taactagtat atatgcacta atccactgtt ttccatgctg
    10921 tccgaacaca aaaagagggt gtggaaaagc cgttttggac aataaattaa ctgcggaata
    10981 tgcacaaata gcgtatgata gcggcagaat ctgttgatga gagcttcatt ctatgaaacc
    11041 tgtttttttg gattttgaac aacccatagc cgaactgacc aacaaaatcg atgagctgcg
    11101 tttcgtccaa gacgagtctg ccgtcgatat ttcggacgaa atacaccgtt tgcagaaaaa
    11161 aagcaacgac ctgaccaaat cgattttcag caaacttacg cccgcccagg tttcacaggt
    11221 ttcccggcat ccgcagcgtc cctatacttt ggattacatt gatgcgctgt ttactgattt
    11281 tgaagagctg cacggcgacc gccattttgc cgacgatcat gcgattgtcg gcggattggc
    11341 gcgtttcaac ggacaaagcg tgatggtcgt cgggcatcag aaagggcgcg acaccaaaga
    11401 aaaaatccgc cgcaacttcg gtatgccgcg ccccgagggc taccgcaaag ccctgcgcct
    11461 gatgaaaacg gctgagaaat tcggcttgcc cgtcatgacc tttatcgata cgccgggcgc
    11521 gtatcccggc atcggcgcgg aagaacgcgg gcagtcggaa gccatcggca aaaacctgta
    11581 cgaactgacg cgcctgcacg tccccgtttt gtgtaccgtc atcggcgaag ggggctcggg
    11641 cggtgcgttg gcggtcgccg taggcgatta cgtcaatatg ctgcaatact cgacctattc
    11701 tgttatctcc cccgaaggct gcgcttcgat tttgtggaaa accgccgaaa aggcggcgga
    11761 tgcggctcag gctttgggca ttactgctga ccgcctgcaa aagctggact tggtcgatac
    11821 cgtcatcaaa gaaccattgg gcggcgcgca tcgggatttc gggcaaacca tgaaaaacgt
    11881 aaaagccgtt ttggaaaaac aactgcacga agcgcaaagc atcccgcttg ccgatttgct
    11941 ttcgcgccgt ttcgaccgca ttatggctta cggcaaattt tcggaacaat aattcaggta
    12001 gaacaagcag caagcagttt gtctgaaact gcttgctttt tctttatcgg gacggaaccg
    12061 tgctgacttt agatgcgttt gagcaatgct tgaaggattg ttttcctcaa ggtctgaatg
    12121 gaaaaaaaac agcagtggca ttaagcggcg gcttggattc cgtcgttttg ctgcatctgc
    12181 ttgtccgcgc cggaaaaaag ggcggtttta ttccggatgc attgcatatc catcacggct
    12241 tgagtccccg tgccgacgat tgggcagatt tctgccaaaa ctattgcgat atgctcgggg
    12301 tggggctgga aacggttaag gtctgcgtgg aaaaaaacgg tttgggcatc gaggcggcgg
    12361 cgcggcaaaa gcgttatgcc gcgtttgccg aaaaaggctt tgacgttttg gcgttggcgc
    12421 accacaggga cgatcaaatc gaaaccttta tgctggcagt cgctcgcggc ggcggtttgc
    12481 gcgctttggc ggctatgccc accgtccgcc cttttgggga aaaaggcatc atctggcggc
    12541 ctttgctgcc tttttcgcgc caagacatat gggattatgc ccaaaaacac ggtttgccga
    12601 atatcgagga cgaaagcaat agcgatacgg cttatttgcg aaaccgcttc cggcaccgta
    12661 ttttgcccga actttcggcg cagattcccc atttcgggcg gcatgtactg aacaatgtcc
    12721 gcgctttgca ggaagatttg gctttgttgg acgaaatcat cggtcaggac tgccgttggg
    12781 tctgtgagga aggcagtttc gatacggtgc gttggcaggc gttttcccca cgccggaaaa
    12841 cccatatttt gcggtatttt ttgaaggaaa acggcatccc catgcagaat cagaatgccc
    12901 ttgccgacat tgcccgggtt ttgacggagg caaaaaccgg acgttggaac ttgcaaggct
    12961 ttgaattgca tcattatgca ggcaggctgt ttgtgttccg actggaaaaa acggataaac
    13021 tgcggttttt gaaagacagg cagataagcg gaaatttaag ggaaatattg acggggcagg
    13081 gatttgtttt aaaacgtcat ccgtttgggc ttcctgagca tcttttggag caggacggaa
    13141 ttttgaggac ggtagcggta tcggatacgt tggctgtcgg cggcatccat aaggatgtga
    13201 aaaaaatcct tcaggggaaa cgggttttgc ctgtcctgcg cccaatttgg ccgcttgttg
    13261 ccgacagcgg aaaccgtcca ttggcgttgg caaactgttg tgcggatttt caaatctcgg
    13321 tttcagacgg cattttgccc gtccatcctg actttcccat tttattttga taatatcgca
    13381 aacagatttc ggcggcgttc agtcgggtat tgtccggttg catatttcta aaaggcttgt
    13441 gaagtgagac acatcagttc gaccaataat gaacacatca gacacctgca ccgcctgttg
    13501 tcgcaaggaa agttcagacg gcaatacgcc caaaccgttt tggagggcgt gcacctgctt
    13561 caggttttcc tgcaatccgg cgggatgccg gtcggggtat atattcccga gggcaaaatg
    13621 ccgtctgaag aaatccgcag gctgagggcg gttgtgccgg aaggcaggat tttttccgtt
    13681 tcagacggca tattgaaaaa aatcagtagc ttgagttgtg cagacgatat acttgcactg
    13741 attgatatcc cattgggcgg aaccttgccg gacaaaggcg actgtgtggt tttggacggc
    13801 gtgcaagatc cgggcaatgt cggcaccgtc ttacggagtg cggcggcggc aggggtaggt
    13861 acggtcgttt tgggcagggg ttgcgcggac gcgtggtcgc ccaaggtatt gcgcgcgggg
    13921 atgggcgcgc atttcttgtc gggcatttat ccgcaggcgg atttggaaat atggttggcg
    13981 cgctataaag gccgtgtgtt tgccaccgcc ttgcgcgagg aaaagcaggc ggttttgtac
    14041 ggcgaagatt tgtgcggacc gacagcctgg gtgtttggca acgaaggcgc gggggtcggt
    14101 aaagcggttt tggacagggc ggacaagtgt gtcaggattc cgatgcacga tgcaaccgag
    14161 tctttaaatg tcgcgatggc ggcgacaatc tgcctgtttg aacagatgcg gcagcggtcg
    14221 gcgtattgag gaaaagaaat gccgtctgaa aaaatctatt acggcgtatt gattttctta
    14281 tgtatcgctt ctatgctgct gtcgccgttt ttttatgcgg gtgctttgaa gcccaagaag
    14341 gcggcattgc ggaaggacgg gcagtggaaa ctcatcttat tgtccaatac cgtggcggcg
    14401 gcggttttgg cttgggtgtg gtggaaatgg ttttgacaga tattgctcaa aatcgtgcta
    14461 atggaatccg aacaaataaa gaatttggta aaaaatttgt taaatcaacg aattaaagtt
    14521 ttgtggaaaa caaaacagct ctaagcaaat agggcgtttg tcggtaaata cggaagagtt
    14581 gcggcattat cgggcatctt taacaagtag tgccgtcttg gcaggcaatc ggtttttatg
    14641 ggcagcttgc aaaatcgcgg atataaaatt gcgaatcggt taaagtgtgg ggacgctatg
    14701 aaaaattgcg aattttttta tgacccgaca agggcaatct atgatagcgg tgcagattac
    14761 ttaactaggg aaaaacatag attagtcgta attgcaaata gtgcttgggg gctattactt
    14821 aatttatctt gttattatga cgaggttttg gaaaagcgga aaataccgtt cggcaaacag
    14881 gaaattgatg acgatatgga caaagtgtcc gcccttaagc ggaagtttaa agatatttct
    14941 gaaatcaaag taggggatgg ttgggaatac ccgttcaatt atgggcaggg aatgaaagaa
    15001 ttagatgaag tgctattgaa atacattccc ttttttgaag aagaacgata aaggaggttg
    15061 atatgcgcgt atctaaaata attggaagta tgttgcttgt tacagcggtt cagaccgtat
    15121 tttcggcaaa tgtttacgcg tgccgccata atggtaaaac cagttacagc caaactccgg
    15181 gaaaacattg taccaacgcg ggtttggggc gggaccgggt gtacagttcg gttagaccgg
    15241 cagtaaaaga cagggcggaa gacgcagggg tcggcgatta ttcggacacg gtgagggacg
    15301 aacacgtcca aaatccgaaa gggaatgcac agaaagacgg ttcggatgcc ggcatcaggc
    15361 cgcattgatt gaaaccgaat cagccatgac ggcgttgaat aatagaattt gaacgattag
    15421 ggaactctga tgaaacacat ccacattatc ggtatcggcg gcacgtttat gggcgggatt
    15481 gccgccattg ccaaagaagc cgggtttgaa gtcagcggtt gcgacgcgaa gatgtatccg
    15541 ccgatgagca cccagctcga agccttgggt ataggcgtac acgaaggctt cgacgcggcg
    15601 cagttggagg aatttaaagc cgacgtttac gtcatcggca atgtcgccaa gcgcgggatg
    15661 gatgtggttg aggcgatttt gaaccgcggg ctgccttata tttccggtcc gcaatggctg
    15721 gctgaaaacg tgctgcacca tcattgggta ctcggtgtgg cggggacgca cggcaaaacg
    15781 accaccgcgt ccatgctcgc atgggttttg gaatatgccg gactcgcgcc gggcttcctt
    15841 atcggcggcg taccggaaaa ctttagcgtt tccgcccgcc tgccgcaaac gccgcgtcaa
    15901 gacccgaaca gccaatctcc gtttttcgtt atcgaagccg acgaatacga caccgccttt
    15961 ttcgacaaac gctccaaatt cgtacattac cgcccgcgta ccgtcgtgtt gaacaatctg
    16021 gaattcgacc acgccgacat ctttgccgac ttgggcgcga tacagaccca gttccaccac
    16081 ctcgtgcgca ccgtgccgtc tgaaggctta atcatctgta acggacagca gcaaagcttg
    16141 caggatactt tagacaaagg ctgctggacg ccggtggaga aattcggcac ggaacacggc
    16201 tggcaggccg gcgaagccaa tgccgacggc tcgttcgacg tgttactaga cggcaaaacg
    16261 gccggacgcg tcaaatggga tttgatgggc aggcacaacc gcatgaatgc gctcgccgtc
    16321 attgccgccg cgcgtcatgt cggtgtcggc attcagacgg cctgcgaagc cttgggcgcg
    16381 tttaaaaacg tcaaacgccg catggaaatc aaaggcacgg caaacggcat caccgtttac
    16441 gacgacttcg cccaccaccc gaccgccatc gaaaccacca tagaaggtct gcgtcagcgc
    16501 gtcggaaacg cccgcatcct cgccgtcctc gagccgcgct ccaacaccat gaaactcggc
    16561 acgatgaagt ccgccctgcc tgtaagcctc aaagaagccg accaagtgtt ctgctacgcc
    16621 ggtggcgtgg actggaacgt cgccgaagcc ctcgcgcctt tgggcggcag gctgcacgtc
    16681 ggcaaagact tcgatgcctt cgttgccgaa atcgtgaaaa acgccgaagc aggcgaccat
    16741 attttggtga tgagcaacgg cggtttcggc ggaatacacg gaaagctgct ggacgctttg
    16801 agatagcccg ggcgatgccg tctgaaagcc cttcagacgg catcgtccgg ctgcgcggca
    16861 caaaggcgga aaaaccgttt gccccgtatt ttcaaacgcg ttacacttgc cgccgctgtt
    16921 ttcagccatt tgattacccg caaccgccgt cattgcgccg gcggtttgcc tgtcagcgtc
    16981 attgcgccgc tgtaaatacg aaagaacaca ttatgaccgt atcccccgtc gccttgcgcc
    17041 gtaagaccga gtgcaagcct catcccaccg cgcgctattg gaaaaaatgc gatgtcgaag
    17101 ccctgttcgg acttcccttc ctcgacctca tttaccaagc cgccgaaatc caccgccaaa
    17161 atttcaaccc gcgcgaaatc cagctttcca cgctgttgtc catcaaaacc ggcggttgtc
    17221 ccgaagactg cgcctattgt ccgcaatcgg cgcaccacaa taccaatctg ggcaaagagc
    17281 agatgatgga tgtggatgaa atcgtcgaaa aagccaaaat cgccaaatcg cgcggcgcaa
    17341 gccggttttg tatgggcgcg gcgtggcgcg gccctaaacc caaagacgtg gagacggttt
    17401 ccgcaatcat caaagccgtc aagggcttgg gtatggaaac ctgcggcacg ttcggtatgc
    17461 tcgaagaagg tatggcggaa gacttgaaag aggcgggctt ggattattac aaccacaacc
    17521 tcgacaccga ccccgaccgc tacaacgaca tcatccacac ccgccaacac gaagaccgaa
    17581 tggacacctt gggcaaagtc cgcaacgccg gtttgaaagt ctgctgcggc ggcatcgtcg
    17641 ggatgaacga aacccgcgcc gaacgtgccg ggctgattgc cagtctcgcc aatctcgacc
    17701 cgcagcccga aagcgtgccg attaaccagt tggtcaaagt ggaaggcacg ccgcttgccg
    17761 atgccgaaga tttggactgg acggaatttg tccgcaccat cgccgtggcg cggattacga
    17821 tgccgcaaag ttatgtccgg ctgtcggcag ggcgcagcaa tatgcctgaa gcaatgcagg
    17881 cgatgtgctt tatggcgggc gcgaactcga ttttttacgg cgacaagctg ctgaccacgg
    17941 gcaatcctga tgaggacggc gacagaatcc tgatggaaaa gctcaacctg tatcccttgc
    18001 agtttgaacc ggaaggcgag gtcgccgaag tggaaaaagc ctctgggatt aaagtggatt
    18061 attgacgatt gaaaaatgcc gtctgaaacc cggaaaaagg ctttcagacg gcatttgtcc
    18121 ggacggcatt tccaatatct ttttaccggc gcgtgatgct gccgtcgggc gagacatcca
    18181 gtccgttccc cttggggaaa tcgctgcggt tcagataaat aatccgcccg ataacggttt
    18241 tctcggggtc gattttgggg attttatcgc cgacttgagt gatggggata atgttaccgg
    18301 aaacgaaccg cccctgtttg tcggtgataa ttttgaaaat cggcgcgatg ccgctgatgc
    18361 cggaggtgtt gattgcgccg taggtggcaa agttgccgcc gctgtaggag atgaagcggt
    18421 cgcggtaaag ttcgacggcg cgagtaacgt gcggcccctg cccgaatacg acatccgcgc
    18481 cggaatcgac ggcaagccgt gcaaactcga cgacgttgcc cctgttttcc ccatagaaga
    18541 tttcggtatc gaacggcagg tgttccgcct gtttcccttc cgcgccgccg tggaacatta
    18601 caatgacgat gtcggctttc tgtttggttt tggtaatcag ttttctaact ttggcataat
    18661 cgttcagttt gacggcggca aggttggggg cgaaggagac gaagccgtat cttacgccgt
    18721 ttttcttcag gatggcggtt tcaaacctgt tttcgatgcc cgaatatttg atgttcaatt
    18781 cgtcaaggtt cgccctttat gccgtttccg caccgcaaac cgccggggtc aagccctcgg
    18841 cgcatcctgc cggaacggaa tccccgtgct gccgattgac ggttcaaacc ccgcgcccgt
    18901 ttcaaatacc ggcgatgtgg acggacagga tgcgcctgac gaaaagacag ccgataccgt
    18961 ttccattatc ggcgtgggcg acattatgct cggcagcaat tatccggtcg attacctgcc
    19021 cgataccaat attctgaaaa acgtcgaatc tgccttgcaa gacgcggaca ttaccgtcgg
    19081 caacctcgaa ggcacgctgt ttgacgaagg cggtacgccg aaaaaatgtg caaaccccca
    19141 aaatatgcta tgcattccga acgccctccg catacgggca ataccttgcc gacgcgggat
    19201 tcgactacct cagcttcgcc aacaaccaca gcaacgactt cggcgcgcaa ggcatcacgg
    19261 caacggcggc gggcggcagc tcttttacat actcaatcgc gctaaagccg ctgccgataa
    19321 cgaggccaaa attgcggaaa ataccgccat cgcccagata aatttgtcca tcatcagacc
    19381 tttactgttc agacgagaca gcatttgccg cacgttttgg ggcttatctt tcgatttgcg
    19441 ctacgtcgcg caccgcgcct ttgtcggcgg aagtcgccat cgcgccgtaa gcccttaatg
    19501 cggcggagac gtagcggtcg cggttttccg gtttccacgc tttgctgccg cgtgcttcca
    19561 tttcggcacg gcgcgcggca agctcttcat cggaaatgac aaggtggatg ctgcggttgg
    19621 ggatgtcgat ttcgacggta tcgccttcgt gtaccaaacc gatcgcgcca ccttccgccg
    19681 cttcgggcga ggcgtgtccg atggacaaac ctgatgtgcc gccggagaag cgtccgtcgg
    19741 ttaagagagc gcaggctttg ccgaggcctt tagatttcag gtaggaagtc ggatacagca
    19801 tttcctgcat gcccgggccg cctttcgggc cttcgtagcg gatgatgacg atgtcgccgg
    19861 cgacgatttg gttgcccaaa atgccttcga cagcggcttc ctggctttca aacacgcgcg
    19921 cgcggccggt gaatttgagg atgctctcgt ccacgcctgc ggtttttacc acgcagccgc
    19981 gttcggcgat gttgccgaac aagaccgcca aaccgccgtc ttgcgagtag gcgtgtgcca
    20041 cgtcgcggat acagcctttt tcgcggtcga ggtcgagtgt tttccacata cggttttgcg
    20101 agaacgcttg ggtggtgcgt acgccgccgg gcgcggcttt gaagcgttcg atggcgcggg
    20161 tgttttcagg gttggttacg tcccattttt caatcgcgtc tttcagcgtc ggtgcgtgga
    20221 tagtgtgcac gtcggtgtgc agcttgcccg ctttgtccaa ttctttcagg atggcgaaga
    20281 tgccgccggc tcggtgcacg tcttccatat agtagtcgtg gttgttgggt gcggttttgc
    20341 agatgcaggg cacaacgcgg cttaagcggt cgatgtctgc cattttgaaa tccacgcccg
    20401 cttcgttggc aacggcgagt aaatgcaaaa tagtgttggt gctgccgccc atcgcaatat
    20461 ccatcgtcat agcgttttca aacgcttttt tggtggcgat gctgcgcggc agtacggttt
    20521 cgtcgtcttg ctcgtaatag cgtttggtga tttcgacaat catacggcct gcttcgagga
    20581 acaattcttt gcggccggcg tgggtcgcca aatacgaacc gttgccgggc agggaaaggc
    20641 cgagtgcttc ggtcaggcag ttcatcgagt ttgccgtaaa catacccgaa cacgagccgc
    20701 aggtcgggca ggcgttttgt tcgacttcct cgacttgccg gttgctgaca ttgtcgtccg
    20761 ccgattcgat catcgcgtca atcaagtcca aacggcgttc gggttggatg tttgccacgc
    20821 cgataacttt gcccgcttcc atcgggccgc cggagacgaa gatggtgggg atgttcaggc
    20881 gcatcgcggc aatcagcatg cccggggtga ttttgtcgca gttggaaatg cacaccagcg
    20941 cgtcggcgca gtgggcgttg accatatatt cgatggagtc ggcaatcaaa tcgcggctgg
    21001 gcagggagta cagcatgccg ctgtgtccca tagcgatgcc gtcgtcgatg gcgatggtgt
    21061 tgaattcttt ggcgattgcg ccggcttttt cgatttcgcg ggcaaccagc tggcccatat
    21121 tgtgcaggtg gacatggccg ggcacgaatt gggtgaagga gttggcaacg gcgatgatgg
    21181 gcttgccgaa gtcggtttcc atcacgccgg tggcgcgcca caatgcacgt gcgcccgcca
    21241 tattgcggcc gtgggtggag gttttggagc ggtattcagg catagtgtgt ttccttgtgc
    21301 ctataccgtc tgaaagacag ggctgtttca gacggtatcg ggtacggttt tttagagtgg
    21361 gaaaagaggg tattttatac caagtatcgg aattttgcgg gattgaaacg gcgtgcggca
    21421 aaaaagaaaa tccccgcagg aatgcgggga cgggttcagg cgcgggcaat cgcgacggct
    21481 ttggatgcgt cccaaaaatc aacgggtgca tttaatacgg gtttgacgat gcccgtccgt
    21541 atggcgaaat cgccgaaacc ttcgccgata ttgcgttctg ccgcccattt gccgatcagg
    21601 tcgtccaatt cggcaaggat ttcgggcagg gtgatgtttt ctttgtaaag acgggggatg
    21661 cgtacgcctt cacggtcgcc gccgatgtgg aggttgtagc gtccgacggc tttgccgacc
    21721 agtccgattt ccgccaacat cgcccgtccg cagccgttcg ggcagccggt aatgcgggta
    21781 acgatgtagt cgtccgacgt gccgtgtttc gccataatct tatccagctc gccgatgaag
    21841 tccggcagca cgcgttcggc ttccgccatt gccagcgggc aggtcggaaa ggaaacgcag
    21901 gacatcgcat tttcacgcag cttgctgaca tcgttgcgga ttaatccgta tgttcgggca
    21961 aattcttcga tttttgcttt gtctgcttcg gcgacatttg ccacgatgag gttttggttg
    22021 gcggtgatgc ggaaatcgcc tttgtggatt ttggcgattt ccaacacgcc ggtcagaagc
    22081 tgtttcccgc cttcgtcaac caaacgcccg ctttcgatga aaagggttaa atgccagttg
    22141 ccgtctatgc ctttcaccca gccgatgcgg tcgccgcgcc cggtaaattt gaacgggcgt
    22201 acgggttcga acggcatacc catacggcgt tcgacttccg cgcggaagtt gtccaagccc
    22261 atattttgaa tggtgtagcg ggtgcgggcg tttttgcggt cgctgcggtt gccgaagtcg
    22321 cgctgcgtgg ttaccaccgc ttcggcggcc ttcagcgcgt gttccggagg cacgaaaccc
    22381 agttccagtg aaatgttcgg ataggttttg gtgttgccgt gttccatcga aagcccgccg
    22441 cctgccaaaa cattgaagcc ggcaagctgt ccgttaccgt ctgaaacggc gacgaaatcc
    22501 aaatcgttgc cgtagcagtc cacatcgttc aagggcggga tgacgactgc ggttttgaat
    22561 tttcgcggca gataggtttt gcccaaaatc ggctcgtctt cttgaaggaa gtcgtcggaa
    22621 ctttgaactt ttttgccgtc cacccacaca tccagataac cgcgcgtgcg cggcagcagg
    22681 tgttcggaaa tctttttcgc gtattcgtaa gcctgccggt gcagttcgga ctcgatcggg
    22741 ttggacgtgc aaagcacgtt gcggttcata tccgccgccg tggcgatgga atccaaaccc
    22801 agtttgtgca agaggcggtg catcgtctgc aacttggctt tcggcacgcc gtgaaattgg
    22861 aaggtttgcc ggttggtcag ccggatggag cggtaatgac tgttttcccg ggcaaatttg
    22921 tccagttcta tccattggga cggtttgatg atcccgcccg gcagccggca gcgcaaaagc
    22981 ataaatttca agggctcgag ttttgcctcg gcgcgttcgg cgcggatgtc gcggtcgtcc
    23041 tgctcataca taccgtggaa gcggatgagt tggaagttgt cgcctttgaa gccgcccgtg
    23101 agcgggtctt tcaaatcgtc caaaatcgtg ccgcgtaaaa aattgctttc ggttttcaga
    23161 cgttcgttgt cggatagcgg tttttcttgc cacgccaaac cttttgtctt ggtctgtacg
    23221 gtcattttgt gttcctcccg attatattta atcaataaac atcacgctga tagcgttttt
    23281 cttcgcgcag catatccaaa tattcttctg cgccctcttc gtccaaatgt cctgccccga
    23341 taatcacatc cagcaaggcg gcttcgacat cttttgccat ttttgccgca tcgccgcaca
    23401 cataaacata tgcgccctcc tgcaaccatt gccaaagctc tgccgcctgt tcgcggattt
    23461 tgtgctgcac atagattttt tcttcctgat cgcgggacca ggcgaaatcg tatctgtgca
    23521 ggaaaccctc tttggcaaac tgctgccatt cggtttgata gagaaaatca cgggcaaaat
    23581 gcggattgcc gaaaaacagc cagtttttgc ctgttgcatt ttctgtggcg cgttgttgca
    23641 aaaaagcgcg aaacggtgca atgcccgtac cggagccgat catcacaatc ggcttgcggc
    23701 tgtcttcggg cagcctgaag ccgtcgttgc gttccacaaa cacgcgcacc gtgccgtcct
    23761 cttccagccg gtcggcaagg aaacccgatg cgccgcccgc cctggcgcgc ccttcgtgtt
    23821 caaaacgaac cacgccgaca gttaaatgca cttcatcgcc cacttccgcc tgtgctgaag
    23881 aaatcgaata caaacggggt gcaagcggac gcagtaaacg gatgaattgt tctgccgtca
    23941 ggcttgccgg gaagcggtgc agcacatcga caataggcgt gttttgcacg aaatcctgca
    24001 aaacggcgtt atcggcaatg attttatcga gttcttcata atgggcgaac gcggcatagc
    24061 ctttgacgaa agccggagtg ttttgcgtga gttcgaaatg agatgaaagt gcgcgcgcaa
    24121 ccggcatcat ctttccgcct gcctgtattt ccgttgccgg atcgatgccg agcaggtcta
    24181 ggatttcccc gaccagtgcc ggattgttgt caaaccaaac gccgagcgcg tcgcccggga
    24241 ggtagtgcaa atccgaaccg ctcaaatcga tttcgatgtg gcgcacgtct ttatcggatt
    24301 ggcgggcggt gattttctga ttgaccagca gggcggcggg aaaggggtct gccttgcagt
    24361 acctgccatc cggtgccgtc tgaaggccgg ctgggggcgt tgtctgcggc gcgggcgttg
    24421 cccggttttt tgcggcttct tcttttaagc gcgcgacaat gtggcctgtc catccgtctg
    24481 cggcggcggc aaagtccaaa tccgcatcaa cgcgttcgag caggcgtttt gcgcccaatt
    24541 cttcaaaacg tttgtcgaaa tctttgcccg cccggcagaa attcggatag gagctgtcgc
    24601 ccaagcccag tacggcaaat tggagtttgt ccaatttcgg ggcttttttg ccgttcagca
    24661 gtttgtgcag cacgacggct tcttccggcg gttcgccttc accctgggtg gacgtaacca
    24721 gcagcaggcg gcgttcgccg gcgatgtttt tcgccttata gtctttcagt tcggcgcgcc
    24781 tgactggtat gccggcggct tccaggctgt ccgccgcttt gtcggcaacg gatttcgcat
    24841 tgccggtttg cgaggcggaa aggacggtta cggaaaaagg ttctgccgcc ggcaatgccg
    24901 tctgaagcgc gggcagtcct gcagatgccc cgtttcctgc ttttgcccaa gcgtagccgg
    24961 acagccacgc ccattgtgcc gcgtccagcc ccgacaggag ctgcgtgatt tcgggcggca
    25021 gaggcggtaa tggcggattt gtgttctgca tatcgtgttc actcataaaa tcatacctgc
    25081 cgcaacagtg ccgtatgtcg cttcgtctat caggataaac gaaccggcgg cggtgttttc
    25141 cgcataaggc gttgccgtaa cgggtttttg aaggttgatg cggactttgg cgatgtcgtt
    25201 catcttcaag gattccgcgc cggcctcttg ttccagcgtg cggacatcca aaacgctttc
    25261 aatttccccg acttttgccg gcacggtttg cgtgccgtgc ttgagcaggt atttgcgcgc
    25321 ggtgttgagc ggacgttcgt caaaccagca aagcgtggct tccagatgtt tttgcggggc
    25381 gagcggggaa tttttatcga caaaaaggtc gccgcgcgaa acatcgatgt cgcgatccag
    25441 ccggagggtt gccgcctcgc cggcaaaagc ctgcgccact tcccctttcg gcgtgatgat
    25501 ttcggacact tcggcggtca gcccgttcgg ttcgatgcgg acggtttgcc cgacggcgac
    25561 cgaaccgcgt tcgatgcgcc cctgatagcc tcggaaatca tcggccttgt cggcatcttg
    25621 gcggacgacc agttgcacgg ggaaataaaa atcgtcggcg gtgcggctga cttcgttcgc
    25681 ccccggcagg gtttccaaaa tggacaataa gggttcgcct ttataccaag gcatattgcc
    25741 gccggggtaa acaatgttgt cgcccaagag tgcggacacc ggtacgaaat gcgcgtcttt
    25801 caaaccgagc tgttcggcaa gtcggcggta tgcctccaca atggcgttga atttgtcttc
    25861 gctgtaatcc agcaggtcca ttttgttgac cgccaccaca atatgcgggc agttgagttg
    25921 gcggaggatg gcggaatggc gtttggtctg cggcagaagc tgcaagggct gcgcgccgaa
    25981 atccagttgg gatgcgtcaa ccagcacgac tgccgccgaa gcggtgcttg cgcccgtaac
    26041 catattgcgc gtgtattgtt cgtgccccgg cgtgtcggcg atgatgaatt tccgtttcgc
    26101 cgtggaaaaa tagcggtatg ccacatcgat cgtaatgccc tgttcgcgtt cggcttccag
    26161 tccgtcggtc aggatggaga agtctatggt ttctttcaaa cctttgcttt tgccggattc
    26221 caaggttttg atttggtcgg acagcagggc tttgctgtcg tagagcagcc gtccgatcag
    26281 ggtgcttttg ccgtcatcga cgctgccggc ggtaatgaag cggagcgggg cctggtgttg
    26341 tgccgtcata ttttcttcct catatctgct taaagggttt ttgaaattta gaaatagcct
    26401 tcttttttgc gtttttccat tgccacctcg cttgcctgat cgtccagccg ggtcgcgctg
    26461 cgttcggaaa tgtcggtaac tgccgtttcc ctgataatct ccgtcggcgt ggacgcggtg
    26521 ctttctaccg gacaggtgca gctgatgtcg ccgacggtgc ggaagcggac atcaaggatt
    26581 tcggaggttt cagacggcat tttcggggtg agcggtgtta cagggaccag cagccccctg
    26641 cgtctgacca cttcgcgcct gtggctgtaa taaatcggcg gcagctcgag gttttcgcgg
    26701 gcgatgtatt gccagatgtc gagttccgtc cagttggaaa tcgggaagac gcgcatattt
    26761 tcgcctttgt gcagcctggt gttgtacagc gaccacagct cggggcgttg cgccttcgga
    26821 tcccattgtc cgaactcgtc gcggaacgag aaaatccgtt ctttggcgcg ggctttttct
    26881 tcgtcgcgcc gcgcgccgcc catcagcgcg tcaaagccgt ttgcctcgat ggtttccaac
    26941 agcgtaaccg cctgcgccgc attgcgcgaa tcggtttctt tgcgtaaaac tactgtgcct
    27001 ttggcgatgg agtcttccac gcgccccact atcaggcggg cattgagttt tgccgcctgc
    27061 gcgtcgcgga aggcaatcac ttcgggataa ttgtgtcccg tgtcgatatg caccagcggg
    27121 aagggcagtt ttaccggcct gctgcccagc cggaaggctt tgcaggcgag ggcgagcagg
    27181 accacggaat ctttgccgcc ggaaaagagc agggcggggt tttcgcattc tgccgccact
    27241 tcgcggataa tgtggatgga ttcggattcc aaccagtcga gttgggcgtt gttcggttcg
    27301 gttttcgtca taccatattc cttatttctt ctgtctgata tttatgaatt atttgtgcag
    27361 cccgcattct ttgctgtttc tgccttccca ccaccaccgc cctgcgcgga tgtcttcgcc
    27421 cgccttgacg gggcgggtgc aggggtcgca gcctatgctg ggaaatcctt gccggtacaa
    27481 atcgttgtaa ggcacattgt tggcgaggat gtatgcccac acgtcgtgtt ccgaccagtc
    27541 gaaaatcggg ttgtatttgc cgatgccccg tccggcatcg tattcggcaa acggcagttc
    27601 cgtgcgtgtg gcggattgtt cgcggcgttg cccggtaagc caggcgtccg cgcctgcaat
    27661 ggcgcggttg agcggttcga tttttcggat gcggcagcat tcgcggcgcg cttcaacgct
    27721 gtcgtaaaag gcaaacctgc ctttgctttc cacataatgc agggcatctt cccgtatcgg
    27781 ttggaaacgc ttgatttgca tatgcggata aacacgttca atcctatcca acaggtttaa
    27841 agtttccgcg tgcagcagcc ccgtatccaa agtaaaaata ccgatattga ggttttctcc
    27901 ggcgataagg tcggtaatta ccatatcttc cgcagcaagg ctgctggcaa accgtgcatc
    27961 ccggtggctg ccggcaatcc ggtgcaggcg ttgtttgagg gtttccgttt tttccgcaag
    28021 ggcggtttcg ctgccggatc cgatatgcgg tatctgccac agggagggtt tgagcagtgt
    28081 cgtttccatt tttcccgcct tatgccgccc gttgtccggc attcagtccg cccaatgcgg
    28141 gatacgcctg cccgacccgg ttttctcctt cgccgttttc accgaaccag gcgagttttt
    28201 cgtgcagccc caccacttcg cctatgacaa tcaatgccgg attcggcgcg gtttcggcga
    28261 gttcggcaag gttggcgagc gtgccggttg cggttttttg agccggcaac gtgccttggc
    28321 tgatgacggc tgccggcgtg tcgggcgagc gtccgtgctg ttgcagccgt tcggcaatca
    28381 gggcggcttt gagcgcaccc atataaatca ccaaggtctg gcggctgcgg gcgagggtct
    28441 gccattcgat gtcgggcgca tccgccttgc ggtggccggt tacgaaaacc gcactttggg
    28501 cataatcgcg gtgcgtgagc gggatgccgg cataggcggt cgcgccgacg gcggcggtaa
    28561 tgccggggac gaccgaaaac ggaatctgat ggcgtgccaa ggtttccaat tcttcgccgc
    28621 cgcgtccgaa cacgaaaggg tctccgcctt tcaaacgcac cacgcgcctg ccttcgcggg
    28681 ccagcctgac cataagcgca ttggtgtcct cttgcggggt gcggtcgccc cgggcgcgct
    28741 tgccgacaaa aatccgttcc gcatcgcggc ggacgaggga cagtatgccg tctgaaacca
    28801 gcgcgtcgta aagcaccacg tctgcctgct ggatttcctg tagccctttg agcgtcagca
    28861 gccccgcatc gccgggaccc gcgccgacca gcgagacgga gccgccttga tcattttgac
    28921 gactttgttc caattggcct gccaattccc gttcggcaag ggtgttttgc cggtttttga
    28981 cgagggcggc gaaacgtccg ttaaactgct tttcccaaaa gcggcggcgt tcggtaacgg
    29041 atttcagttt gtccttgacg gcatcgcgcc accttcctga aatttccgcc atatcgccca
    29101 aagacggcgg cagcagggct tccagccttt cacgcagcag tcgggcgagg acgggcgcgc
    29161 tgccggagct ggaaacggca atctgaaccg ggttgcggtc gataaccgac gggaagatga
    29221 agctgcaatg gtcgcggtcg tccaccacgt tgaccggctt ttggcagctt tcggcaagat
    29281 ggaaaacgcg ccggttgagg gcttggtcgc tgcttgccgc aatgatgagg aaaactgtgc
    29341 ggatgtgttc ggcacgaaat tcttcggcaa gccacaggat tttgttttcc gccgccaacg
    29401 cggagagttc ggcattcagg tgttttgcgg caaccctgac ctctgcgccc gccttcagca
    29461 gcaggctgat tttgcgtgcg gcgaccgcgc cgccgcctac gaccaatacg gggcggccgg
    29521 cgaggttggc gaaaataggg aaataattca ctggctgact cctttgctgt ttgcccgcac
    29581 cttgtttccg atacggtgcg tcgcggcatt tttgtcggaa tgcgggtcat tttagacaaa
    29641 aggattttcc cggttaaata ataaaaaggc atttgttaga agctgaaagc tatatgaggg
    29701 cggctgcgga tgcggcggtt ttccg
//
LOCUS       DAWGJV010000005        26432 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWGJV010000005 DAWGJV010000000
VERSION     DAWGJV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.005"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(92..880)
                     /locus_tag="WHQ94_000148"
     CDS             complement(92..880)
                     /locus_tag="WHQ94_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ2127503.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WHQ94_000149"
     CDS             complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WHQ94_000149"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ2127504.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1741..2349
                     /locus_tag="WHQ94_000150"
     CDS             1741..2349
                     /locus_tag="WHQ94_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ2127505.1"
                     /translation="MAAYRTDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRTLKHLADLGMVRPLANLMVQNPPDRLADECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="WHQ94_000151"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="WHQ94_000151"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ2127506.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="WHQ94_000152"
     CDS             2889..3011
                     /locus_tag="WHQ94_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ2127507.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="WHQ94_000153"
     CDS             3008..3727
                     /locus_tag="WHQ94_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ2127508.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRINDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASASSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3781..4107
                     /locus_tag="WHQ94_000154"
     CDS             3781..4107
                     /locus_tag="WHQ94_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2127509.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="WHQ94_000155"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="WHQ94_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ2127510.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="WHQ94_000156"
     CDS             4773..4910
                     /locus_tag="WHQ94_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127511.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="WHQ94_000157"
     CDS             4936..6792
                     /locus_tag="WHQ94_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2127512.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFIWSNMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="WHQ94_000158"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="WHQ94_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ2127513.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7981..8205
                     /locus_tag="WHQ94_000159"
     CDS             7981..8205
                     /locus_tag="WHQ94_000159"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ2127514.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8189..9085
                     /locus_tag="WHQ94_000160"
     CDS             8189..9085
                     /locus_tag="WHQ94_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ2127515.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9194..9493)
                     /locus_tag="WHQ94_000161"
     CDS             complement(9194..9493)
                     /locus_tag="WHQ94_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ2127516.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9605..11050)
                     /gene="nuoN"
                     /locus_tag="WHQ94_000162"
     CDS             complement(9605..11050)
                     /gene="nuoN"
                     /locus_tag="WHQ94_000162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ2127517.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11060..12556)
                     /locus_tag="WHQ94_000163"
     CDS             complement(11060..12556)
                     /locus_tag="WHQ94_000163"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ2127518.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12645..14669)
                     /gene="nuoL"
                     /locus_tag="WHQ94_000164"
     CDS             complement(12645..14669)
                     /gene="nuoL"
                     /locus_tag="WHQ94_000164"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ2127519.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14718..15059)
                     /locus_tag="WHQ94_000165"
     CDS             complement(14718..15059)
                     /locus_tag="WHQ94_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127520.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15183..15758)
                     /locus_tag="WHQ94_000166"
     CDS             complement(15183..15758)
                     /locus_tag="WHQ94_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEZ2127521.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(15792..16097)
                     /gene="nuoK"
                     /locus_tag="WHQ94_000167"
     CDS             complement(15792..16097)
                     /gene="nuoK"
                     /locus_tag="WHQ94_000167"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ2127522.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16094..16765)
                     /locus_tag="WHQ94_000168"
     CDS             complement(16094..16765)
                     /locus_tag="WHQ94_000168"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ2127523.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVYRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(16777..17256)
                     /gene="nuoI"
                     /locus_tag="WHQ94_000169"
     CDS             complement(16777..17256)
                     /gene="nuoI"
                     /locus_tag="WHQ94_000169"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ2127524.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(17337..18413)
                     /gene="nuoH"
                     /locus_tag="WHQ94_000170"
     CDS             complement(17337..18413)
                     /gene="nuoH"
                     /locus_tag="WHQ94_000170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ2127525.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(18416..20677)
                     /locus_tag="WHQ94_000171"
     CDS             complement(18416..20677)
                     /locus_tag="WHQ94_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ2127526.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDVESLPSKLDNRNTWTGEGVQTTSDRLVRVGGVGIYHTDSIVRRSAPLQETGH
                     AAVPAARVNPNTLARLGLQNGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(20967..21404)
                     /locus_tag="WHQ94_000172"
     CDS             complement(20967..21404)
                     /locus_tag="WHQ94_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127527.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(21404..22705)
                     /gene="nuoF"
                     /locus_tag="WHQ94_000173"
     CDS             complement(21404..22705)
                     /gene="nuoF"
                     /locus_tag="WHQ94_000173"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ2127528.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(23095..23568)
                     /gene="nuoE"
                     /locus_tag="WHQ94_000174"
     CDS             complement(23095..23568)
                     /gene="nuoE"
                     /locus_tag="WHQ94_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ2127529.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(23568..24824)
                     /gene="nuoD"
                     /locus_tag="WHQ94_000175"
     CDS             complement(23568..24824)
                     /gene="nuoD"
                     /locus_tag="WHQ94_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ2127530.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(24814..25407)
                     /locus_tag="WHQ94_000176"
     CDS             complement(24814..25407)
                     /locus_tag="WHQ94_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ2127531.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(25420..25902)
                     /locus_tag="WHQ94_000177"
     CDS             complement(25420..25902)
                     /locus_tag="WHQ94_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ2127532.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(25893..26249)
                     /locus_tag="WHQ94_000178"
     CDS             complement(25893..26249)
                     /locus_tag="WHQ94_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ2127533.1"
                     /translation="MLSAYFPVFVFIFIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFIYEWKKGALEWE"
BASE COUNT         6569 a         7441 c         6730 g         5692 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtctcc agcgcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa cggcggtgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 agaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgaacg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacatgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctctcg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag gcacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaac atgatacgta cgctgaaaca cttggcagat
     1921 ttgggcatgg ttcgcccatt ggcaaacctg atggtacaaa acccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcac aacgctacgg ctggcagata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattctgt taatcgacga tgtctttacc accggcgcga cgctggacga actggcaaag
     2281 acgctgaaaa aatcgggcgc gaaccgcatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc ccatgcagcc cggcagtcgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgca tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cgcacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatcttcat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gcttcggcat caagcccgaa
     3601 cctcgccatt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc gcgcggtatg gttcgggcgg tattgactgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacacggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacagcaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcatacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaaaac attccgccaa ctgatcaagg tcggcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagacgtga aacgcctgtc ttccgcgccg ggaatcggca aaaaaaccgc cgaacgtatg
     4561 gtcttggaac tgcgcggcaa gctggtcgcg catacggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcagcacgc tgcttgcgct gggttacagc
     4681 gaacgcgaag caaaagcggc ggtcaaaggc gttccggagg ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgtc tgaaggcggc gcgacgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaatc ttttcctggt tcgaatcccg aatcgacccc
     4981 tatcccgaag ccgccccgaa aacgcctgaa aaaggactgt ggcggtttat ctggagcaac
     5041 atggctgggg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgtt tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccccatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctcgactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggttgct acagccaccg ccatggcgtt gcgcgtcaac
     5881 ggtttgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc gaacgccctg
     6001 ccgctgaaag tgtcgcaagg cgaaatcaag ttcgaacacg tcgatttctc ctacgaagcg
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgcc cgggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttc gatcgacggg caggacataa gcggcgttac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccagagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 tgtccgcacg cccgacggca caacctacga agccagcacc cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgcgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctacaacgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc ggcagcctgc gccccgccct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc gcaaaccggg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcatca ccgacgaact gcccgggtaa cgccttgccg cctgggcgga
     7741 aaaatgccgt ctgaagccgg attcgggttt cagacggcat ccgtttttca aaaatgctac
     7801 aatccgcttt ttactggaac atccgaaact atgttccaac acgcaggacg acacttaaag
     7861 caccgcccta tgtgtcgtcc tgatttggaa gggcttacac ccctcccaaa taaagtctga
     7921 ttctaccgcc ccgaaggaca gatgtccaag tggcggggtt tcaaccgaaa aggaaacacg
     7981 atgaagaaaa acgccccgaa atcgtttgaa gaagccttgt cgcgccttga atcgctgaca
     8041 caagctatgc agggcgaaat gcccttggaa gacgcgcttg ccgcctatca ggaaggcaac
     8101 gagctggtca gatactgcca aaccaagctg gcgcaagtcg aacagaaatt acaggttttg
     8161 gacgcggacg ggccgaagga gttgaacctt gaatccgacg aatgatttga aagcgtggca
     8221 acagagggcg caggcgcaaa cagagctgct gctggaacgc tttttgccgt ctgaaaacga
     8281 aataccgcac acactgcacg aagccatgcg ttatgcggtt ttggatgggg gcaaacgtct
     8341 gcggccgatg ctggttctgg cagcttcgga attaggcgaa gccgtgcatg aagcagtaga
     8401 acaggcaatg gcggcaatcg aaatgatcca tgcctattcg ttggttcacg acgatatgcc
     8461 ggcaatggac aacgacagcc tgcgtcgggg caaaccgacc tgccacatca aatatggcga
     8521 agccaccgcg ctactggtcg gcgacgcttt gcaaacccaa gccttcgacg tgttaagccg
     8581 tccgacagaa ctgcccgcag cacgccagtt ggcaatgttg tcggtgttgg cgaaagcggg
     8641 cggcagcgcg ggcatggcgg gcggtcaggc aatcgatttg gcgaatgtcg gcaaacaaat
     8701 ggttcaagcc gatttggaac agatgcacag cctgaaaacg ggtgcgctaa tccgtgcggc
     8761 ggttttattg ggggcgatgt cctgcccgaa tctgtctgaa gacagtctgg cggtattgga
     8821 cgattatgcg gcaaaactgg ggctggcgtt ccaagtcatt gacgatgtgt tggattgtga
     8881 agcggacacg gcgactttgg gcaagacggc gggcaaagat gcagacaacg acaagccgac
     8941 ttatgtgaaa ctgatgggct tggaagcggc gcgctcatac gcacacaaac tggttgccga
     9001 agcggtcgcg ctgctcgaac ccttcggcga caaagccctg cgcctgcggc agttggcaga
     9061 atttgcagtc gcccgcaaat attaaaaccg gcgtatatcg ccggctgaaa cgtgccggca
     9121 cagcgatgcc ggcctgcact tgaatgaaac aaatgccgtc tgaacaggtt tcagacggca
     9181 tttctatgct tgtctattcc ctgttgcgcg tgtgccaaaa ataccgccac acaaaacatg
     9241 gaaacgcaaa aatcaggtac aggcagacga ctgtggcata aaactcccaa ccctgatcgt
     9301 gtaccgatcc tgcacgggat tcgagaatat aggcaagaat ggcggtcagt gcgaaacctg
     9361 ccgccagctc gatcatgtgg tgtccgaaat gtttgcgctt gagcgcggcc acgccgaaca
     9421 gtctggtggt gaggaagggg gcgttggcaa agatgagtgc caagaccaaa aggatgtaca
     9481 tggatgcggt catggtgggc tttctgtttg ggcattgtcg gcggcgatgc cgtctgaact
     9541 gcattgcgga acggcatatc ttaacaaaaa cggcagcctc tgacacggct gccgttgcgg
     9601 cggcttacag cgtgttctcc aacgccttgg cgcaccagtc gataacggtt tgcggcataa
     9661 tgccccacag caccaacagc agcgcgttga tgcttaagag gaattttgcg gcattgttgc
     9721 cgccggcggc gggacgggcg cggccggatt cgtcaaaata catgactttg accacgcgca
     9781 ggtagtagaa cgcgccaatc agcgacatca cgacggcaaa tacggacaac caaacatagc
     9841 cttgttttaa gagtgccata atcacgccga atttggcgta aaagcccatc agcggcggaa
     9901 tgcccgccat agagaacata accagcagca tcaaaaaggc aagccatacg cggtgttggt
     9961 tcaaccctgc caaatcgctg atgttttcgc actcgttgtc cccgtccgac aacaccatca
    10021 acacaccgaa ccctgccgcc gccatcagcg cgtaggtaat ggcgtaatag agacccgccg
    10081 caaagccgac cgcgcccgcc ataaacgcca gcaggataaa ccccatatgc gatacggtgg
    10141 aataggcgag catacgtttg atattggtct gcatgatggc ggcaaggttg ccgaccagca
    10201 gcgaggcggc ggcaagcagg gcaaacatca gagaccagtc atgatgcacg gttcccaacc
    10261 cggtaacgag gatgcggaaa gcgaaaacaa cggcggcgat tttcggggca gtgccgacca
    10321 gggcggtaac agaagtgggc gcgccgtgat acacgtcggg cacccacata tggaacggca
    10381 ccgcgccgag tttgaacgca acggcgacga cgataaacac cagacccagt ttcaacagcc
    10441 attcgtttgc ttcttcattg aaggaagagg cgagcacgcc ggcaaattcc agcgaaccgg
    10501 ttgcgccgta aaccatagaa ataccgtaga gcagcaggcc ggatgccagc gcacccagaa
    10561 caaaatattt caaggcggct tcggcggcaa agccggaatc gcggcgcagg gcaatcaggg
    10621 cgtaaagggc aagcgacaag agttccaaac cgatataggc agttaaaaaa tgccccgcgc
    10681 tcaccatcac gcccataccc aagagggcaa acagcgacag ggtgtaaaac tcgcctttga
    10741 aaataccgcg cacttggttg tagggcttgg catagacaaa cagggtaaac atcaaggcat
    10801 ataaaaccat ttttgccaaa cgcgacatac cgtctgcaat atacatcccg ttgaacgaag
    10861 acgtgctgcc ctgttcccac accgccaact gcaccacagc cgtaaccgcc accgttgcca
    10921 acgcgccgta atgcgtccac cggcgtttgt catcactgac ccacaagtcc gccagcaaca
    10981 ataacaccag cagcgcaagc agcacgattt cgggcatggc gggcattaaa ttcaaatcag
    11041 accagttcat ttacacacct caaatcttgc tttgtgccac atgggcaatc aaatcgtttg
    11101 ccgcctgatg caccacttcg ataaatgcgt tcggatacag gcccataccc aataccgcaa
    11161 ccgccaaaac cgccagaatc gtaaactcgc gacagttgat gtctttcatt tcggcaacgt
    11221 gcggattgtg aatcgcgccg aaaatcacgc gcttgtacat ccacagggta taagacgcac
    11281 cgtaaatcag cgtcatagcc gccaacgcgc ccacccagaa attcacttta accgcgccca
    11341 taatcaccat aaactcgccc acgaagccag aagtcgccgg cagaccggca ttggccatac
    11401 cgaacaacat cataaacgcc gcaaacttag gcatcacatt gaccacgccg ccataatcgg
    11461 caatattgcg ggtatgcagg cggtcgtaca tcacgccgat acacataaac atcgcggcag
    11521 acacaaaacc gtgggaaatc atttgaatga ttgcgccttt caatgcccaa tcattcaatt
    11581 ggccgttcac aaacaggaac atacccaaag tgacaaaacc catatggctg atggacgaat
    11641 acgccaccag ttttttcata tcggtttgca ccaaagccac cataccgatg taaatcacgg
    11701 caatcaggct caatacaatg atcacagggg caaaatagcg tgccgcatcc ggcataatcg
    11761 gcaggataaa gcgcaagaaa ccatacgcac ccagcttcag cgtaatcgcc gccaacacca
    11821 tcgaaccgcc ggtcggcgct tcaacgtggg catccggcaa ccaagtgtgc acagggaaca
    11881 tcggcacttt tacggcaaat gacaggaaga acgctgcaaa taaaagctgt tgtacgccca
    11941 acggaatctg tttgatgttt tggaaatcga caatagagaa gctgcctgtt tgatagtaca
    12001 ggtaaaccat agcaaccagc atcaggagcg aacccatcag cgtatagagg aacagcttga
    12061 ccgacgcata gacgcggcgc gggccgcccc atacaccgat aatcaggtac agcggaatca
    12121 gcataccctc gaagaacaca taaaacagaa tcgcatcctg cgcggcaaac gcaccgttaa
    12181 tcaaacccga catcatcagg aatgccgcca tatactgcgc cgggcgtttc tgaataactt
    12241 cccaacccgc cagcaccacc aacagcgtaa taaacgcatt caagatgata aagagcactg
    12301 aaataccgtc cacgcccaat gcatagttga ttttcagcag cggaatccac tcgtggaact
    12361 cggtaaattg atagccgccg ctcaaacggt cgaaaccggt aaacaggggc agcgttacca
    12421 agaaaccggc aagcgcaccc ataaaggcaa gcacacgcgc cagcggcgca cggctgtcct
    12481 taccggtcga caaaaccagc acacctgcgg cgatgggtat ccatattgcc aagctgagta
    12541 ggtagttgga aaacatagtg gttaacctgt ggttaaaata aaaatatcgt tgtacgggtt
    12601 ggtttgtcag acgacgtttt aagagaccgt ctgaaacctc atccttatct gaacaatccc
    12661 cagaaggtca taccgagcaa gaccaatacg ccgaacacca tagcggcggc gtaggtatag
    12721 ataaagccgg tttgagcttt acgcacttgc gcggcaatcg cgccgaccag tttggcagag
    12781 ccgttgacga tgccgttgtc gataatggcg gtatcgccga ctttccagaa gaaagtaccc
    12841 aatgcgcgtg tgcctttggc gaaaacgttg aaatacaggg cgtcgaggta gtatttgttt
    12901 tcaaacaaaa cgtaaatcgg acggaacgtc tgtgcaattt tcgctggcag gtgcggcagt
    12961 ttgacgtaca aaagccatgc gctcaacacg cctgcgatag caaggtagag tacaggcgaa
    13021 tgcaggctgt gcgacaccat tgccaatgcg ccgtggaact cttccttcat gatgtgcatg
    13081 gtcggatgcg cgtcggcgtt gacgaaaatc acatctttga agaaatcgcc gtaaagcata
    13141 ggctcgatgg caacgtagcc gatgatgacg gacggaatgg caagcaaaat caaaggcagg
    13201 gtaacaacca acgggctttc gtgcggattg tcgtttttgc ccaagctgtg atgttcttcg
    13261 ccatgatgat cgtcatgatg ttcaggcaag ctgcgccatt tctcttcgcc gtggaacacc
    13321 ataaagtatt ggcggaacgc gtagaacgcg gtaacgaaca cgctggcaag gacggcaaaa
    13381 taggcaaagc cgctgcccgg cagggtgctg tatttcgccg cttcgataat cgaatcttta
    13441 gaatagaagc cggagaagaa cggcgtaccg atcagcgaca agttaccgat cagcatggtc
    13501 agccaagtaa tcggcatgta ttttttcagg ttgcccatgt ggcgcatgtc ttggtcgtgg
    13561 tgcataccga taatcgcgct gcctgccgcc aagaacaaca aggctttaaa gaaagcgtgg
    13621 gtcatcacat ggaacatcgc cacggaatag gcagacgcac ccaaagccac ggtcatatag
    13681 cccagttgcg acaaggtgga atacgcaacc acacgtttga tgtcgttttg aatcacgccc
    13741 aagaaaccca taaacagggc ggtaatcgca ccgatcacca taatgacaga cagcgcagtg
    13801 ctgctcatct cataaatcgg cgacatacgc gataccataa acagaccggc ggtaaccatc
    13861 gtcgcagcgt gaatcaatgc agaaatcggg gtcgggcctt ccatcgaatc aggcagccaa
    13921 acgtgcagtg ggaattgtgc cgatttaccc atcgcaccga caaacaggag caaacaggtg
    13981 acagtaatca acgaccattc cacaccgggg aaaagctgga tggtggcatt ttgcacgttg
    14041 ggcagataag cgaatacatc ttgatagcgc aggctgccgc cgaaataggc aagcaccaag
    14101 ccgataccga gcaaaaagcc gaagtcgccg acacggttga tcaaaaaggc tttcaggttg
    14161 gcaaatgttg cgcttggacg tttgaaatag aaaccgatca agagatacga caccaagccc
    14221 acagcctccc agccgaagaa gagctggatg aagttgttgc tcataatcag catcaacatg
    14281 ctgaaagtaa acaaagaaat atagctgaag aagcgttggt agccgacttt ttcatcgtgc
    14341 atatagccga tggtgtagat atgcaccatc aacgatacgc ccgttaccac gaccatcatc
    14401 atcgccgtca tcgtatcgac caagaagccg acggagaaat ccaaaccgcc catcgtcagc
    14461 caggtataga cgttctcatc aaacttggca cggctgccat tgaggaatcc ccacagcaca
    14521 taagccgaca gcacggcaga caccgccaca ccgagtatcg taacagtatg cgcaccggca
    14581 cgaccgattt tgttgccgaa caaacccgca atcaacgagc ctgccaacgg aacaagggca
    14641 attatcaaat ataaagtcat gtcgttcatt tgattgaatc cgattgattt aaaaatctat
    14701 gtttgtttcg tacaaaatta cttcggaaaa acaaatccaa cacgctccaa tcgtttgcgt
    14761 gccacagcta attgctcttc agtaaataaa tcacaccacg gcttttgtaa caccagatat
    14821 tccatactgt atttcaaggc gtggactccg ccaccactca gaatcagctc tgtaaaaccg
    14881 gtctgagtct tcttttcccc cgtacttaat aatttatccg ccgcctcttt accaccaaat
    14941 tcatttacaa tttgtaaaaa tcgtgtcgcc ttgtaaggtt gcggcaaatt caaagcctcc
    15001 tgataaatat ttaacatggc tttatgaaat tcttgttcta actgattttt atccatcatt
    15061 cttcttccaa tatttcagac cggattattc ttacccagaa tttcttttct catccgctcc
    15121 cgtctgatca cctaccgaat caggtcgtct gaaacagtct gaaatcgctt ttcagacgac
    15181 cctcagcctt tttcataccc ttcgtaataa tacgactgct cgataccttt aaagatgatt
    15241 tcacggttgt ccacatcgtc ggtcaggttg tcctttaaca gaaagcgcag ttctaaatcg
    15301 ttgacggggc tgcgttccat cgcctgcaaa tacagggttt tgcttacatt ttgccagttt
    15361 acgatttttt tcaggttttt cttcagcacc aaatccagcc agatgcgggt acttctgcca
    15421 ttaccctcca aaaacggatg ggcaatgttc atttcaacat atttggcgat gatttcttca
    15481 aaagtccgct cgggcatctg ctcgatttta accaaagcct cttttaaata catggcgttg
    15541 gcaaaacgaa aaccgccttt ggaaatgttg tcttccctga tttgacccgc aaaatcatat
    15601 aagccgccga acaggtaacg gtgaatctgt tgcaggcccg cggtggtacc gacttcgata
    15661 cggtcgatgt cgccgctttc aaacaggcgg cgggcattat gcaggctttg ttcgtctatg
    15721 gatttcatcg tttttcctat cgggttttca gacggcatcg gtgtcttgtc aggtctcgac
    15781 ccaacctacc tttacccttt caactcgtcc aaatcggcaa cattaatcgt ttgtcggttg
    15841 cggtacacca ataccataat cgccaaaccg atggcagatt cggcagcggc aacggtcaat
    15901 acgaagaata cgaaaatttg tccggcagta tcgcccaaat gttgcgagaa ggcgataaag
    15961 ttgaagttca ccgccaagag catcagctcg atggacatca gcaataccag cacgtttttg
    16021 cggttcataa agatacccat tgcgctgata ccgaacagga gcgcacccaa taccagataa
    16081 tgcgtcaagg taatcatgct ttgccctccc cttccggctt gaggccgtct gaaacttcgc
    16141 tctcttcggc agattcgact tgcggtttga ccgcttccat tttcaccaga cgcatacggc
    16201 cttgatcggc gcgtacttta acttggtcgg caggatccat gcgtttcgga ttggtggttt
    16261 tacggtaaac cagcgcaatc gccgccacca tacccaacag cagcaatacc gccgccaatt
    16321 caaacggcaa caggtagtcg gtataaatac ggctgcccaa atcgcggata ttgttgtaat
    16381 cggccggaat gtctttcatc agaccaaatg cggccaggtc ggttttcggg ttgaccagaa
    16441 tcaggatcag ggcaaccgcc aacaatgtgc cgaccacacc ggcaaccggc gcatgacgcc
    16501 agaaaccggc gcgcatttct tcaatgtcga tgttcaacat catcacaacg aacaggaaca
    16561 acaccatcac ggcgccgacg taaaccacca ccagcgtcac gcccaaaaac tcggcctgca
    16621 tcagcatcca aagcatcgcg ctcacgcaga aggtcagcac cagatgcaaa gcggcgtgga
    16681 cagggttttt ggcggtaacg gtacgaaccg caccgtacaa aacgatggcg gcaagaatat
    16741 agaacagaat cgcggaaaaa gtcattcgtc tgctccttaa cgatacggcg cgtcagcggc
    16801 tttgcgtttg gcgatttcag cttcgtattt gtcgccaatg gccaaaagaa tcggcttggt
    16861 catgtgcaag tcgccttttt tctcgccgtg gtattcaaaa atatgggttt ccacaatcgc
    16921 atcggtcggg caggcttctt cgcagaaacc gcagaagatg cacttggtca ggtcgatgtc
    16981 gtaacgcttg gtgcggcgcg tgccgtcttc gcgttcttcc gattcgatgt tgatcgccat
    17041 tgcggggcaa acggcttcac acaatttaca cgcgatgcag cgttcctcgc cgttcggata
    17101 acggcgttgc gcgtgcagac cgcggaaacg cacggattgc ggcgttttct cttcgggaaa
    17161 ataaattgtg tctttgcggg caaaaaagtt tttgagcgtt acgcccatgc cttttaccaa
    17221 ctcgccgagc agaaaggttt ttactaagtt agccatatta tgttccctca aaacagggat
    17281 ttcgttaggt attcaaaatc gctttgttca gacggcctca agatgccgtc tgaaacttat
    17341 ttccacaaat tcagcggtga aatcatccac acgcccaaaa tcacgatgta ggcgaagccg
    17401 atcggaatca gcactttcca gcccaaacgc atgatttggt cgtaacggta gcgcgggaag
    17461 gttgcacgga tccacagata ccagtacaac actgccgcca ttttcacaaa catccagaat
    17521 gcggaaggcg taccgataaa gccccagctt tgcgggaacg gagacaacca gccgccgaga
    17581 aacatcagcg atgtcagcgc ggcaatcaga atcatgaaaa tgtattcggc aaggaagaac
    17641 agcgcgaatg cgaagccgga atattcaacg tggtgtccgg caacgatttc ggactcgccc
    17701 tctgccacgt caaacggtgc gcggttggtt tcggcaacgg cggaaatcag atagacgatg
    17761 aagattggga acagcggcag ccagttccaa gagaataccg aaccgcccgc gatacctttt
    17821 gcctgcgcgg caacgatgtc ggagaagttc atgctgcccg acaccatcac gacacacacc
    17881 agcgcagcac tcatggcaat ctcgtaggaa atgctttgtg cggaagcacg cattgcaccc
    17941 aagaacgaat atttggagtt ggaagcccag cccgcgatga tcacgccgta aaccgacagc
    18001 gaggtaatca tcaggatgta caaaaggccg atattgatgt tggtcagcac ccattcttcg
    18061 ttgaacggaa tcactgccca cgccgcgaaa gacggggcga gcgacataat cgggccgatg
    18121 tagaacaggg ctttgtttga cagcttcgga cgggttacct ctttaaacaa gagtttgaac
    18181 acgtcggcaa acggctgaat cagaccccac gggccggtta cgttcgggcc gacacgaagc
    18241 tgcataaagc cgatgacttt acgttcgaaa tacgtcaggt aggcaacggt cagaatcagc
    18301 ggaatcagga taatcacgat tttgacgatg acggatacca ccaaacctac ggtaataccc
    18361 aaatcgccca gaccgagcgt tgcggcaaag aggttttgga accattcctg cataatcaag
    18421 ctcccgccag ttcaatagtg tccatcaacg cacccagcgc ggcattttcg gtatgcagcg
    18481 gcagatgcac cacgttttca ggcaaaccgg catcggcttt gacggcaacc gatacgcttg
    18541 cgccgttttg tttggcgaca gcagtttgtc cgttttgcag gcccaagcgt gccaaagtgt
    18601 tcggattgac acgcgcggcg ggcacggcgg catggccggt ttcttgcaac ggtgcggaac
    18661 ggcgcacgat agaatcagtg tgataaatac cgacgccgcc gacacggacg aggcggtctg
    18721 aggtcgtctg aacgccctcc cctgtccatg tgttgcggtt gtccagtttg gacggcaggc
    18781 tttctacatc cagcgcatct ttcagaatcg cagcggtatc gtggtattca aaaccttgca
    18841 ggtcaaacag gttgcccaat acgcgcaata ctttccacat cggacgcgaa tcgccgaagc
    18901 cttgtaccac gccgtggaag gattgcagac ggccttccat attgatgaag ctgcccgagg
    18961 tttcggtaaa cggcgcaatc ggcaacaaca cgtcgcacac atccagcagc gtttcgctga
    19021 caaacggtgt aaacgccatc acgcttttcg cctgtttcaa cgcggccacg gctttcgcac
    19081 cgtccgccgt atcgatttcc ggttcgacgt tgagtagcaa gaccgcctgt ttcggcgcgt
    19141 ttgccatttc ggcaacgctc tcgcccgagt tcacgcccaa gacatccgca ccaacgctgt
    19201 tggcggcttg cggcaaaata cccagcactg cgccggtcgc ggctgccagc tcttgcgcgg
    19261 cagcataaat ggcggcgtaa tcaggatggt tttgcacttc cgcgcccaaa atcaccgctg
    19321 ctttttcagc atttttcagg ctggcggtaa cggcgtgttc cgcattggca gacaggtttt
    19381 tcagacggcc tgcccactcg tcgggatgtg cggcttcttg agagagaagc ggcatcaaca
    19441 actcttcctt gctgccggcc aatacgctca aggccatacg gtctttggcg gcgcggcgca
    19501 ggcgggcggt caggagcggc tgttctttgc gcaagttcgc accgactacc aatacggcat
    19561 cgttgtcagc caaagattca atgctttgtc ccaaccattg tgcaccttta aggtcgtctg
    19621 aaagacgttt gtcttgttgg cgcagacggg tggcaaagtt tttaacaccc aagccgtcgg
    19681 caagcttttt ggccaaatac agctcttcaa ccgtattcat cggattggcc caaacgccga
    19741 cttgattttg gttgccgtct ttggcgatac atttaattgc gctgtggaca tattccaacg
    19801 cggttttcca atccacgtcc atccactcgc cgccctgttt gattttcggg tttttcaaac
    19861 ggctttcgtg atacaggcct tcataggcaa aacggtcgcg gtcagacagc caacattcgt
    19921 taatcgcttc gttttccagc ggcaacacgc ggcggacggt atggtctttg gtttgtataa
    19981 tcaggttgct gcccaaagcg tcatgggcgg aaacggattt gcggcggttc aattcccaag
    20041 tacgcgcgtt gaagcggaac ggtttgctgg tcaatgcgcc gacaggacac aaatcgatga
    20101 cgttgcccga cagctcggtt tccaccgttt tgccgataaa gggcatgatt tcggagtgtt
    20161 cgccgcgatt caccatcgca atttcctgca aaccggcgat ttcttcagtg aaacgaacgc
    20221 agcgggtgca gtggatacag cggctcattt cctcggcgga aaccaaaggc cccatgtctt
    20281 tgccgacgac ggaacgtttt tcttcggtgt agcggctggt ggtttttccg tagcccaccg
    20341 ccaaatcctg caactggcat tccccgcctt ggtcgcaggt cggacaatca agcggatggt
    20401 tgatgagcag gaactccatc acgccttcct gcgcctctcg ggcttttgcc gaatgcgtac
    20461 gcacaatcat gccgtctgta accggcgtgg cacaggcagg caggggtttt ggggcttttt
    20521 ctacgttcac cagacacata cggcagttgg cggcgatgga aagttttttg tggtaacaga
    20581 aatgcggaat ataagtaccg agcttgtgcg cggcttcaat caccgtcgcg ccctgctcca
    20641 cagatacttg tttgccgtcg atttcgattt gtaacatggt tcgttcctag ttacgggtat
    20701 ttgataaata atcttttatg gaggtcgtct gaaaaatggg tttcagacgg ccttttgagt
    20761 ttcattgaca atcaatactg tttaaaacgt ttactgccac cttgccgtca ttcccgcaca
    20821 ggcgggaatc cattttttga atttcggcaa ctgcttttca aatatcgggt tctgtaaatt
    20881 ccactatgga ttcccgccta cgcgggaatg acggcaaagt taaattttta gcattttgct
    20941 tttaaccaat ataaaaccaa ctaaaactat aactcattta ttatattttt aaatgactta
    21001 aataactctt taacagcacg ctcattgtct tcatctgtag gctcaaactt ttcacaccaa
    21061 ttaattatat ttacatcgcc atgcaataac tctgcataac atcctactgc atcgcaacta
    21121 ccaccccgtg tccttgacgt gccaaaaata aaatcacaat tatgcgaata aagtttgtat
    21181 aaatttctcc taacctcgta ttcattatca ccagatgaat aaaaaccaat tctctgacct
    21241 tcatgatcaa atactgcaac aaaatctaag ccatttccat gtctttcaaa aaagactaga
    21301 aatttagaaa atttccaaca aatctgatta aaaagcgtat tgagtgtcgt actcttacct
    21361 ttgttggctg caccgtatag aataaaaatt ttagcttcca ccattaccac cctccccact
    21421 tatgctcttt catcggtccg ccgtgttcga tgtaatgcac aaactcatca cggaaatgct
    21481 tggtaaagct gcgaacaggg aaaacggcgg catcggcaag ggcgcagatg gtgcggcctg
    21541 ccatttggtt gccgacagaa tccagcaaat ccaaatcttc catgcggcct ttgccttcta
    21601 cgatgcggtg gacgatgcgg taaagccagc cggtgccttc tcggcaaggc gtacattggc
    21661 cgcaagactc gtcgtagtag aagtagctca ggcgctcaag ggctttcacc atgcacacgt
    21721 cttcgtccat gacgataatc gcgccagaac ccagcatcga gcctgctttg gaaatcgagt
    21781 cgtagtccat atttgtctgc atcatgatgt cggcaggcaa tacgggcgcg gacgaaccgc
    21841 caggaataac ggccttgagt tttttgccgc cgcgcatacc gcctgccatt ttcaagactt
    21901 cggcaaacgg tgtgcccaat ggcacttcat agttgcccgg acgctcgaca tggccggaaa
    21961 tacagaataa tttggtaccg cctgcattcg gaataccttt atcggcaaat gcctgtccgc
    22021 cgtcacggat aatgaatgaa acggaggaga aagtttcggt attgttgatg gtagtcggtt
    22081 tgccgtacag gccgaacgaa gcagggaatg gcggtttaaa gcgcggctgg ccttttttgc
    22141 cttccagcga ttcaagcaat gcggtttctt cgccgcagat gtatgcaccg tagccgtggt
    22201 gggcgaagag ttcgaattca aaatccgaac ccaaaatatt tttacccaaa aagcctgcgg
    22261 cacgcgcctg ctccaaagcg gcctcaaagc gttggtagcc ttcaaaaatt tcgccgtgga
    22321 tatagttgta accggctttc gcgcccatcg cgtaaccggc gataatcatg ccttcaatca
    22381 gggcatgggg gttgaacata ataatgtcgc ggtctttaaa tgtacccggc tcgccttcgt
    22441 cggtgttgca aacgacgtac ttttcgccgg ggaaagaacg gggcataaag ctccatttca
    22501 aaccggtcgg gaagcccgca ccgccacgac cgcgcaagcc ggaggttttg acttcgtcaa
    22561 tcacatcggt ttgcgagatg ttttcggaca ggattttacg cagggcggta taaccgccac
    22621 gtttgacgta ttcgtccaat gtccagcaat cgggattggc ggtatccact tggtcaaaaa
    22681 tcacgcctga ttggtaaata gccatttttg gtgtgcctgt ttgttttcgt atcggttgcg
    22741 gccgctgttt cagacgacct taagatgtct ttgtgtaccg gcttgtaacg tcgtctgaaa
    22801 taaaatctag tttatcaaat cgatattttc agacgacctt taatcgtttt tgttggatct
    22861 cgaccacttg cttgtctgtc gtcattcccg cgcaggcggg aatccatcct caatggtaag
    22921 caatgtctta ttaaattcag aaaccgaatc ttaccggtgg attcccgcct gcgcgggaat
    22981 gacggcattt cggtatttca gtagggcgga ttcttaaatc cgacattttg cctttttacc
    23041 cactctgtcg gctacaagta tccgacctac gtttaaatcg tcgtttcaga cgacctactc
    23101 caactccgcc agtttcttct caatcgcttc ttcggtcata aagctgcaca tgctgtggtt
    23161 gttgaccagc ataacgggag cgtcgccgca tgcgcccatg cattcgcctt cgacaagggt
    23221 aaacttgccg tcaggggtag tttcgccgta gccgataccg agtttttgtt tgaggtattc
    23281 gccggtagcc ataccgccgc gcagggcgca gggcaggttg gtacaaacgg tcagtttgta
    23341 tttgccgaca ggctcaaggt cgtacatatt gtagaaagtg gcgacttcgt aggcttgtgc
    23401 aggcgtgatg ccgatatagt cggcaacaaa agcgatggtt tcgggggcga gccagccttt
    23461 ttcggtttgg gcgatacgca acgcacccat gatggcggaa cggcgttggt cggcgggata
    23521 tttcgccagt tctgtgtcga tttgttttaa ggactctgcg gataacatta tcggtcaacc
    23581 tccccgaata cgatgtcctg cgtaccgatg atggcaacga cgtcggcgag catgtggcct
    23641 tttgccattt cgtccatgcc ttgcagatgg gcgaagccgg gtgcgcggat tttcaggcgg
    23701 tagggtttgt ttgcgccgtc tgaaatgatg taaacgccga actcgccttt cggatgttcg
    23761 acagcggtgt aggtctcgcc ctcgggaacg tgcataccct cggtaaagag tttgaaatgg
    23821 tgaatcaggt cttccatacc tgttttcatt tcggtacgtt tgggcggagc gaatttgtgg
    23881 tttgtggtaa tgaccggacc cggattgaca cgcaaccact cggaacattg tttgatgatg
    23941 cgtacggatt gacgcatttc ttccatacgg cagaggtagc ggtcgtagca gtcgccgttc
    24001 acgccgacag ggatgtcgaa atccattttg tcgtacactt cgtaaggctg tttcttacgc
    24061 acgtcccatt ccacacccga accgcgcaac atcacgccgg taaagccttt ttgcatggcg
    24121 cgttcggggg agacgacgcc gatgccgacg gtacgctgtt tccagatgcg gttgtcggtc
    24181 aggagggttt cgagtgtgtc gatatttttg gggaagcgtt cgcagaaggc atcgataaag
    24241 tcgagcatgg tgccttcgcg ggattcgttg agctgcttca atactttggc attgcggaat
    24301 ttgctgctct cgtatttagg catgaagtcg ggcaggtcgc ggtaaacgcc gccgggacgg
    24361 aagtaggctg catgcatacg tgcgccggac acggcttcgt acaagtccat cagctcttcg
    24421 cggtcgcgga aggcgtagag gatggcggtc atcgcgccga tgtcgaaggc atgcgaaccg
    24481 atgcccatca agtgattgag gatgcgcgtt acttcggcaa acatcacacg gatatactgg
    24541 gcgcggatag gtacatcgat accggcaagt ttttctactg ccaaacaata cgcctgctcg
    24601 ttgaccatca tggaaacgta gtccaagcga tccatatagg gcagggcttg caaataggtt
    24661 ttggtttcag ccagtttttc agtaccgcgg tgcaggaggc cgatatgtgg gtcggcgcgg
    24721 acgatttgtt cgccgtccag ctccaaaatc atacgcaata cgccgtgcgc cgcagggtgt
    24781 tgcgggccga agttgatggt gtagtttctt aatttattgg ccaccgtagt tctcctcacg
    24841 gacgatacgc ggcgtgatct cgcgcggctc aatggtaaca ggttggtaaa tcacgcgttt
    24901 ttgctcttcg tcgtaacgca tttccacata gccggaaatc gggaagtctt tgcggaacgg
    24961 atgtccgaca aagccgtaat cggtcaggat gcggcgcaag tccgggtgat tgttgaacat
    25021 gatgccgtac atatcgaagg cttcgcgttc gtaccaatcc gcgctgttgt aaatatcgac
    25081 tacggattcg actacgggga agtcgtcgtc tgaaacccag acgcgtacac ggatgcgttg
    25141 attgttttta acggaaagca gctgactgac gacggcaaag cgtttgccct gccatgcttc
    25201 gtttttgtaa gtgctgtaat cgacaccgca caagtcaacc agaagctcga aatgcagttc
    25261 ttcatggtcg cgcaatgcgg tcataactga aatatagtgt tcgggcagac actcaacggt
    25321 aatctcgccc aaagctgaaa tgactttgct tgcctgatcg ccaagcacgc cgacaacggt
    25381 ctcgtataag ttttgaatgc ttgccatatc gtcctctcct tattcgtcac gcgcgatggt
    25441 ggaagtgcgc ttgatttttt gttggagctg aatcaggcca taaatcaggg cttccgcagt
    25501 cggcggacaa cccggcacat aaacgtctac cggcacgacg cggtcggcac cgcgcacaac
    25561 ggaataagaa tagtgataat agccgccgcc gttggcacac gagcccatag acaataccca
    25621 gcgcggttcg gcgagctggt cgtatacgcg gcgcagggct ggcgccattt tgttggtcag
    25681 cgtacctgcc acaatcatca ggtcggcctg acgtggggac ggacggaaaa tgatgccgaa
    25741 acggtcaagg tcataacgcg ccatacctgc atgcatcatt tccacggcgc agcaggccaa
    25801 gccgaaagta accggccaca atgaaccggt acgcatatag ttcaacaccg tatccgcgct
    25861 ggtggtgatg aaaccttttt tcaaaacgcc ttctattccc attccagcgc accttttttc
    25921 cattcgtaaa taaagcccac cgtcagaacg acgataaaca ccagcataga ccagaagccg
    25981 tacgcgccca attctttaaa caccaccgcc cacggcagca taaacgcgac ctccaaatca
    26041 aacaggataa agaggatggc gacgaggtaa tagcgcacgt cgaacttcat cctggcgttt
    26101 tcaaaggctt caaaaccgca ttcgtaaggt gcgtcttttt cggcatagtg acgtttcgag
    26161 cccaaaattg tgccgagcag gataaacagt acgccggccg cgaggccgat gaagataaag
    26221 acaaagacgg gaaagtaagc ggacaacatg gttacaccca aatccgttaa caaaatttct
    26281 acaataattt cgtattttag cgaatttcaa aaaccattaa aaggtaaata tcggcaaaac
    26341 gcccaaaaaa acccaataaa tacaatcatg ttatgataac gattcttatt tgattttata
    26401 aggtcatata aatttttaca ccgtaattcg tg
//
LOCUS       DAWGJV010000006        26231 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWGJV010000006 DAWGJV010000000
VERSION     DAWGJV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.006"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(34..753)
                     /locus_tag="WHQ94_000179"
     CDS             complement(34..753)
                     /locus_tag="WHQ94_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2127534.1"
                     /translation="MDTFLDRLKFLWPYGAKPSDIYNKIEMSASGFNRVWKEGAIPTA
                     DYLVKIQEVTGCDLNWLLTGKGLPYLDKARPENAGAFSVSRNSDGITDTMGNPVDLSE
                     FVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWIDNYVTKQPEKLSVITVKGDSMEG
                     VLNHGDSILINHAETDPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDL
                     SNAQDDIAIIGRVEWYGRSID"
     gene            975..1178
                     /locus_tag="WHQ94_000180"
     CDS             975..1178
                     /locus_tag="WHQ94_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ2127535.1"
                     /translation="MKKKQESMTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKA
                     LDAPYLKGERIIAAAIGVPAEEI"
     gene            1300..3288
                     /locus_tag="WHQ94_000181"
     CDS             1300..3288
                     /locus_tag="WHQ94_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2127536.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRS
                     AKVGERTLDGWISAYLKAENAIERLVALAPKTTKAVKPIESYGWLPMFMRFHNIPSAP
                     KLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYSPKAYREIRLEQDGIAPD
                     MLSAEELATMFMPQEVRKVQRGWLDLFSNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAAWDELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(3355..3612)
                     /locus_tag="WHQ94_000182"
     CDS             complement(3355..3612)
                     /locus_tag="WHQ94_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ2127537.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEERSLTDSFVLAWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLEIVESAQGLA"
     gene            3658..4830
                     /locus_tag="WHQ94_000183"
     CDS             3658..4830
                     /locus_tag="WHQ94_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2127538.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YAEKGGNYETIETKIEAFLEMQKSKARREELVLGFVATKTTRRIAEVMRNAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDAVKHLLPDSVQALITVIGFNETLE
                     LVRLMGGTTYPLRQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALY
                     ELRNRKIRSQFDRQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            4832..5353
                     /locus_tag="WHQ94_000184"
     CDS             4832..5353
                     /locus_tag="WHQ94_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127539.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            5496..6041
                     /locus_tag="WHQ94_000185"
     CDS             5496..6041
                     /locus_tag="WHQ94_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2127540.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTVKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGATKV"
     gene            6038..6241
                     /locus_tag="WHQ94_000186"
     CDS             6038..6241
                     /locus_tag="WHQ94_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127541.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNRH"
     gene            6244..6408
                     /locus_tag="WHQ94_000187"
     CDS             6244..6408
                     /locus_tag="WHQ94_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127542.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            6411..6647
                     /locus_tag="WHQ94_000188"
     CDS             6411..6647
                     /locus_tag="WHQ94_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127543.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            6619..7038
                     /locus_tag="WHQ94_000189"
     CDS             6619..7038
                     /locus_tag="WHQ94_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127544.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            6935..7240
                     /locus_tag="WHQ94_000190"
     CDS             6935..7240
                     /locus_tag="WHQ94_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127545.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(7285..7455)
                     /locus_tag="WHQ94_000191"
     CDS             complement(7285..7455)
                     /locus_tag="WHQ94_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ2127546.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            7468..7686
                     /locus_tag="WHQ94_000192"
     CDS             7468..7686
                     /locus_tag="WHQ94_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127547.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            7683..8021
                     /locus_tag="WHQ94_000193"
     CDS             7683..8021
                     /locus_tag="WHQ94_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127548.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            8033..8299
                     /locus_tag="WHQ94_000194"
     CDS             8033..8299
                     /locus_tag="WHQ94_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127549.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            8296..8493
                     /locus_tag="WHQ94_000195"
     CDS             8296..8493
                     /locus_tag="WHQ94_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127550.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            8496..9002
                     /locus_tag="WHQ94_000196"
     CDS             8496..9002
                     /locus_tag="WHQ94_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ2127551.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEISQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(8999..9511)
                     /locus_tag="WHQ94_000197"
     CDS             complement(8999..9511)
                     /locus_tag="WHQ94_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127552.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            9523..11145
                     /gene="terL"
                     /locus_tag="WHQ94_000198"
     CDS             9523..11145
                     /gene="terL"
                     /locus_tag="WHQ94_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ2127553.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            11145..12713
                     /locus_tag="WHQ94_000199"
     CDS             11145..12713
                     /locus_tag="WHQ94_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ2127554.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            12700..14025
                     /locus_tag="WHQ94_000200"
     CDS             12700..14025
                     /locus_tag="WHQ94_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ2127555.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            14153..14569
                     /locus_tag="WHQ94_000201"
     CDS             14153..14569
                     /locus_tag="WHQ94_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ2127556.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            14800..15861
                     /locus_tag="WHQ94_000202"
     CDS             14800..15861
                     /locus_tag="WHQ94_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127557.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPEGAALSADVFAQLAEK
                     DSRIAALSAQNGTPDLTRYAPVSLVRDLQNQVAALTAEREADKGGELITAALSAGKLL
                     PAQKEWAEGVLKQPGGFAFLSGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            15962..16432
                     /locus_tag="WHQ94_000203"
     CDS             15962..16432
                     /locus_tag="WHQ94_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127558.1"
                     /translation="MQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMR
                     RRMQAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGSLNALVAAAINQKP
                     PLIVRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            16422..17567
                     /locus_tag="WHQ94_000204"
     CDS             16422..17567
                     /locus_tag="WHQ94_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ2127559.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            17564..18232
                     /locus_tag="WHQ94_000205"
     CDS             17564..18232
                     /locus_tag="WHQ94_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ2127560.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRTAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            18333..18680
                     /locus_tag="WHQ94_000206"
     CDS             18333..18680
                     /locus_tag="WHQ94_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ2127561.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            18694..19749
                     /locus_tag="WHQ94_000207"
     CDS             18694..19749
                     /locus_tag="WHQ94_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ2127562.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVRDGEAQLMAAPAGVATECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDEAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            19746..20306
                     /locus_tag="WHQ94_000208"
     CDS             19746..20306
                     /locus_tag="WHQ94_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ2127563.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            20317..22293
                     /locus_tag="WHQ94_000209"
     CDS             20317..22293
                     /locus_tag="WHQ94_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ2127564.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNGLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRHRDGLLEALQD"
     gene            complement(23051..23353)
                     /locus_tag="WHQ94_000210"
     CDS             complement(23051..23353)
                     /locus_tag="WHQ94_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ2127565.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(23325..23612)
                     /locus_tag="WHQ94_000211"
     CDS             complement(23325..23612)
                     /locus_tag="WHQ94_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ2127566.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            23692..24297
                     /locus_tag="WHQ94_000212"
     CDS             23692..24297
                     /locus_tag="WHQ94_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127567.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            24276..24572
                     /locus_tag="WHQ94_000213"
     CDS             24276..24572
                     /locus_tag="WHQ94_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ2127568.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            24569..24778
                     /locus_tag="WHQ94_000214"
     CDS             24569..24778
                     /locus_tag="WHQ94_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127569.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(24888..26130)
                     /locus_tag="WHQ94_000215"
                     /pseudo
     CDS             complement(24888..26130)
                     /locus_tag="WHQ94_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         6784 a         6640 c         7315 g         5492 t
ORIGIN      
        1 ttaaaactgt tttaagaggt ttttaaaatg gatttaatca atactccggc cataccactc
       61 aacacggcct atgatggcga tgtcgtcttg ggcattgctc aaatctattt caaacggtgc
      121 gtaacgtgga ttttcagacg ttacaagcag tttgcccggt atacgttgca cacgtttgac
      181 aaagaggtca ttgcctatac gcaagacata taggccgtca cgcgggtcag tttcggcgtg
      241 gttgatgaga atggaatccc catgattgag cacgccctcc attgaatcgc ctttaacggt
      301 aattacagac agtttttccg gctgtttggt cacatagttg tcaatccaat atttccggaa
      361 agccaagcag aataaaggtt cttcgccgaa gactggtgcg ccataccctg ctgctgcggc
      421 tacgttgtag cgcggcacga atacaaactc ggacaggtcg acaggattgc ccatagtgtc
      481 ggtgattcca tcagaatttc tacttacaga gaatgctccg gcgttttccg gcctggcttt
      541 atcgagatac ggcaagcctt ttccggtcag cagccagttt aaatcacaac cggtaacttc
      601 ttgaattttt actaggtaat cggctgttgg gatagctccc tctttccaaa ctctattaaa
      661 tccagaagcc gacatttcta ttttgttata gatgtcagat ggcttagccc cataaggcca
      721 aagaaatttg agcctatcta aaaaagtatc catagtaatc ctaatttaac tcatttaagc
      781 aaaacattaa gcaaaaaaag aaactctttt gcttaaataa gattactcaa ataatcaata
      841 ttttgtaaaa ataattacgt ttttgagaaa atattttagc aaaagagttt catgaagctg
      901 ttttgctaaa tgtaattcac tcatttgcta aatgacggcg gttaataaac ctacttaatt
      961 aaggaattgc gagaatgaaa aaaaagcaag aaagcatgac tgattggcat cgtgctgaca
     1021 ttgtggctcg tcttaaaaag gcaggctggt ctgtgagagc gttatctatc gaggctaatt
     1081 tagcaccaaa tacattaggg aaagctttag atgctcctta tctgaaaggc gaaagaatca
     1141 ttgcagcagc gattggagta cccgcagaag aaatctagcc atctcgtttt gagaaacgaa
     1201 accataagcc aaccttccca agatctataa atagataact gttttgctaa atagttccaa
     1261 aagagtaccg catttaagca aaaatagaaa gcggaaaaaa tgaaaatatc tgcatctgat
     1321 attgcgaaat taggaattcc gagcctacca actgatagac aagggattga ataccatgcc
     1381 aagaaaaata attggcaaca ctgttttgag caaaaaggaa gaggccgtcc taaaaaactg
     1441 tatgaaatcg cttccctccc tgccgaaatc cgagcagcca tcatgaaacg gcagtcggac
     1501 gagctggcgg agaagatgcc gaaaatgctg ccccaagtca gaccagggac ggcgatgtcg
     1561 gctcaggcac tggctgaagc ggccaagctg ttgaacgaga aacaacggtc ggtggcggat
     1621 gcgcgatgtg cggtggtggc ggcggtattg gggattaaat acgaatacga ttgctctgcc
     1681 aaggctgcgg tggctcagtt tttgggcttg ctggcagaag gtaaattgga cgcggtcacg
     1741 cttgggaact tggaaaaggc caatgaccgc agccggtcgg cgaaggttgg cgaacgtact
     1801 ttagacggct ggatttctgc ttatttgaaa gcggaaaacg cgatagagcg gttggttgct
     1861 ttggctccga agacgacgaa ggcggtcaag ccgattgaga gctacggttg gttgccgatg
     1921 tttatgcggt ttcacaacat cccttcggct ccgaaactgg cgcacagcta ccgccggttt
     1981 gtgcagtggg cggaagcgga aaatatgccg gtcaatgatg tgcctaattt gagtatggtg
     2041 cggcgcgttt gggacaagct cccgctaatt atgcaggagc gcggcaggaa aacgggggcg
     2101 gcttataaat cgctgctgcc ttatgtgaaa cgtgattggg gggctttgaa gccgaacgat
     2161 gtttggatcg gcgacggcca cagctttaag gcgaaggtgg cacaccctgt acacggcagg
     2221 ccgtttaagc cggaagtgac ggtgattatt gatggttgta cgcggtttgt ggtcgggttt
     2281 tcggtttcgt tggctgaaag ttgtgtggcg gtatcggatg ctctgcgtat cggggtcaag
     2341 cattttggtt tgccgattat ctattactcg gataacggcg gcggccaaac cggcaagacg
     2401 atagaccatg aaatcacggg tattacgtcc cgactgggta tccgccatga aacgggtatc
     2461 gcgggcaacc cgcaaggtcg aggcatcatc gagcgatggt ggaaagacaa tctgattgag
     2521 atggcgcgcc agtatgagac gtttgcgggc gcaggaatgg acagtagcac gaagaatctg
     2581 atgtaccgca agatggaaag tgcgtttaac gctttggaaa aaggcaagga tttaacggag
     2641 gaacagcaaa aatatttgaa aaaactgccg agctggtcgc gttttatcgc ggatgtggtc
     2701 aagtgtatcg atgattacaa caaccgtccg cacggcgagc tgccccgaca tccggacggc
     2761 gggcattatt cgccgaaggc ttatcgggaa ataaggctgg aacaggacgg tatcgcgccg
     2821 gatatgttgt cggcggaaga gctggcgacg atgtttatgc cgcaggaagt gcgaaaggta
     2881 cagcgcggtt ggctggattt gttcagcaac tcttatttct caaccgagct ggcggagtat
     2941 cacaaagacg aggtacgggt cagctacgat ttgagcgatg cgtcggcggt caatgtgttt
     3001 gatatggacg gcaagtttat tactaaggcg caggccaacg gcaatacccg cgaggctttc
     3061 ccgacagcgc gtatcgacca actggcggaa aaacgccgaa aaggcaaaat caagcgggcg
     3121 gaaaatgcaa tcaagctcgc gaatgcggaa gtcaatccgg cactggaaca ggcggcagct
     3181 tgggacgagc tgggacattt gggcggaaac gtcatcgagg cggagtatgc ggtattgccg
     3241 aaaacgggca cggacgacgg gattttcttg tttgaggcgg atatgtaaag gaaaacatga
     3301 tggaaaaaca gaaaaatgcg gcgttttcgg ccgagcttgt tgaaaaattg aaactcaagc
     3361 gagcccttgg gcggattcaa cgatttcaag caaagattca aggtgttccc gctgaacgga
     3421 atcaggctca aacgtttttg cctgcgcttg aaggaaactg cgaacctgct caatcgaagt
     3481 cggctcttga cgggtaatcc gctggagcag ccaggcaagt acgaaggaat cggtaagcga
     3541 cctttcttcc aagtcttgaa cggcgacttc cagcatgatc aggcgttttt ccaaatcggg
     3601 aaactctttc atttcagacg gcctttaaag gttgtttaaa actcaaggat attaaaaatg
     3661 aaacaaatta atcaagcatt acaacaaaaa ctggttgaat ttaaagaaaa atcgggtatg
     3721 aaccaaaccc aactggcacg cggtatcgat acttcgccgg catccatcag tatgtatctg
     3781 aacggcactt atgcggaaaa aggcggcaat tatgaaacca tcgagacgaa aatcgaggcg
     3841 tttttggaga tgcagaaaag taaggcgcga cgcgaagaac tggtgttggg ttttgtagcg
     3901 actaagacga cccgccgaat cgcggaagtg atgcgcaatg cgcacgaagg cggcgaaaca
     3961 gtggtgatct acggtcaggc gggattggga aagactcagg cggtcaaaaa ctactgcgag
     4021 aaaaacccag cggccatctt gattgaggct aatccgagct ttacggcttt ggtcttgatg
     4081 cgcaagttgg cgacggcggc gaaggtatcg gcgatgggca gcctgaatga tttgtttgag
     4141 tctgtatctg accgcctgcg cgattcgggc cgtctgattg tggtcgatga agcggaaaac
     4201 ctgcccttgc gcgcccttga gattatccgc cgactgcatg atgaaacggg ctgcggcttg
     4261 gtgttgagcg gtatgccccg actggtggcc aacctgcgcg gtaagcatgg cgagctggtg
     4321 caactttata gccgcgtgtc tgttgcgctg aatttgggcg aatctttgcc ggatgacgag
     4381 ctctttgaga ttgcgaaagc ggctttgcct gatgcggtca agcatctgct ccctgatagt
     4441 gtacaagcgt tgattacggt catcgggttt aatgaaacgc tggagctggt gcgcctgatg
     4501 ggcggtacga cttatccttt gcggcagggt tatacgaaaa acagtcaatc ccgtgttgca
     4561 tacttggaag agattatcgg cagtgaggcg gccggtcggc tggtggaggc aatggctccg
     4621 tgcaatctgt ttataccccg ttgcgagacg gccttgtatg agttgcgaaa ccgtaaaatc
     4681 cgcagtcagt ttgaccggca gacggcaggc ggtacccctg cttatgaggc cgttaacgat
     4741 ttggccttgg cacaccgcct aagcgaccgc catgtgtggc gaattttaaa gcaggcggat
     4801 aaggaagcgg agcaggagaa tttgttttag aatggaatgc catgcagatg tatggcattt
     4861 tattttggag aaaaatatga aaaagtttta ttttgtgctg ctggcgttgg gtttggcagc
     4921 gtgtgggcaa gaacaatcgc agaaagctga tgcggagcag tatttttttg ccaataaata
     4981 tcaatttgca gatgagaaac aggcttttta ttttgaacgc gccgcccgtt tccgtgtatt
     5041 gcaacaaggc cttggcgggg attttgagag gtttttaaaa ggagaaatac ctaatcaaga
     5101 aaatcttgca aagtatcgtg aaaatattac tcaagcagtc gcttattatg cggacacgaa
     5161 tggagatgat gacccatacc gcgtctgcaa acaggctgcg caagatgcag aaatcctgat
     5221 gaagagtatg gtaacaagcg gtggaggcgg tacaactgat ttagataagg aaagttatca
     5281 aaattaccga aaatcaatgc aagaatgccg taaaacaata acggaagctg aagccaattt
     5341 gccgaaaaaa taaaataaac gattctaagg ccgtctgaac aacaggcggc ttttttgttg
     5401 cctactgaca ctgtttcgcc cgctgcaaaa gccatgccgt ttgaaaatgt aagcctctga
     5461 aagtgcattt taatctgatt ttgagggagg ctttaatgag caaaattatt tgtctgactg
     5521 ccggacacag taacaccgac ccgggcgcag tcaacggcag cgaccgtgag gcggacttgg
     5581 cgcaggatat gcgcaacatt gtggcttcaa tcctgcgtaa cgattacggc ctgaccgtac
     5641 gcaccgacgg cgaaggcaaa ggcaatatgc ccttgcgcga tgcggtcaag ctgattcgcg
     5701 gctcggatgt ggcgattgag ttccacacca atgcggcggc gaacaaaacg gcgacaggca
     5761 tcgaagcctt gagtaccgtt aaaaacaaac gctggtgtca ggtgctgggc aaagccgttg
     5821 ccaagaaaac cggctggaaa ctgcgcggcg aagacggctt taagccggat aacgcagggc
     5881 aacattcgcg cctggcttat gcgcaggcag gcggcattgt gtttgagccg ttttttatca
     5941 gtaacgacac tgatttggcc ttgtttaaga cgaccaaatg gggcatctgc cgcgcgattg
     6001 cggacgcgat cgcgatggaa ttgggagcga cgaaggtatg aaaaagtctt tgattgcttt
     6061 atgtgttgcc cattgtgcaa agttgaaaaa cgattttggc gtaccaccgt tacctgaaat
     6121 caaaatcacg ccaagccctg ttcgggtagg ctctttgaaa caacatccga gcctgcgctt
     6181 gggtaaatca ggcgtggcag ccgcgaaacg tgcggcgcgc aaacgcaaga atcgtcatta
     6241 atcatgggac aggttgcgtt ttacgaaaag atgattgggc tgtggtcggc caaaagccgt
     6301 gaggcaagcg aacaggcaga cttggcggcg tttgaatttg cggagggcga actggccaat
     6361 tatcaggaaa tgctgaaacg gcacctgcaa accaaaagtg tggaatagca atgcgtattt
     6421 tggatatttt taaaaaccca gcgacaggca atgtgtcgca ctcgaaactg tgggcaaacg
     6481 ttgcctgcgc ggcggggacg gttaagtttg tgatgctgcc cgacccgtcg gcggagattt
     6541 gggcggtgta tttgggcatt gtcggcggct atgcggtggc gcgttcgttg gtcagcgtca
     6601 aacgtcagga ggtcgagaat gaatctcgtg aaactgctgg cgaataactg gcaaccgatt
     6661 gccatcatcg cgcttgtcgg cacgctcttg gcggtgtcgc accatcaagg ctacaagtcg
     6721 gcttttgcga agcagcaggc ggtcattgac aagatggaaa aagacaaagc gcaagccctg
     6781 ctgttgtcgg ctcaaaacta cgcccgcgag ctggaacagg cgcgtgcgga agctaaaaaa
     6841 tatgaagtca aggcgcacgc tgtcggcatg gctttggcga aaaaacaggc ggaagtcagc
     6901 cgtctgaaaa cggaaaataa aaaggaaatc gaaaatgtcc ttactcaaga ccgtaaaaat
     6961 gcaggcggcg gttgtattga cggctttggc catcacggct tgcagctcta caagcgcgcc
     7021 ctcggctacg gaaattaagg ttgtcgaaaa ggcggtcatg ccgacaccgc ctgccgcatt
     7081 gatggtcgcg ccggtacgcc caaatccgcc gaaagacggc aagacggcca cgctgttgga
     7141 acacgccgcc gagtttggcg gctatgtggc ggagttggaa aatcaaaatc aggcttggcg
     7201 cgactgggcg ggcaatcact cccgcaaagt cggaaactga caaaaaagcc cgcgtagggc
     7261 gcgggctgag ggtgaaagcg gattttatac ctcttttaca ggggtagcgg cggtagtgct
     7321 tttcagcaaa tcgactgcgt gctgacagtt ttgcttgctg gtgtagcctt cgccctgagc
     7381 gatgatttca tggttggctg ctttcaaacg ccaacggtat tcgccttttg cgtctttata
     7441 gatttcaaaa tacataaggt ttctcctatg aatgagtaca cgttttctta ccgctttaac
     7501 ggcaagtcct ggtcattgag catttgggcg gacaaccctg aagaagccag ggcgaaattt
     7561 cgggctgcac gagaaaatgc gcactatgac ggcgaagttg tagcaaaggt ttatacattt
     7621 gtaaatattt cgtgggttaa gaaattgtac aagcggacaa aatatttaat gggtatcaaa
     7681 gaatgaccta ccgtgaatta gttgaacgtc agttggctgt gcgccatgcc gatttggaat
     7741 tgggcttaag ccgcgcacgc gagcaagagc cgtttgtcat tcatgtttcc gatctgttgg
     7801 ataaggcagg cattgagtac gcggtacgca tggataagga ttttcagacg acgtttcacc
     7861 ttgaatatcc aattacgaac tatgacacct ttaaacgtgc ggtttggcaa actttggggg
     7921 cgtattactg tgtttgtaat gatggtgatg gactggagat tgccagcaat cgccctgacg
     7981 gttacgccgt ccgtatcgta ttcggcgatg tgccggttta aaggggtttt aaatggactt
     8041 tgaatttggt ttcagaaccc tgtggccgat tgcgacggcg gcattttggt tttgggtcaa
     8101 cggcatttca ggccgcctga aagaggcgga caagcgtatc gacgacctta aagaggagtt
     8161 gcacgcggtc aagctctctt atcacaccaa ggcggacgcc aaggcagaca gcactaatat
     8221 tgcggcggcc ttggagcgaa ttgaaaacaa gttagaaaaa gtaaacgaaa aactggacag
     8281 gaaagcagac aaatcatgag cgacccgatt ttggaagcct tggcgcgtat tgaaaacaag
     8341 actgatcaaa cgctgaaaaa tcagaaagaa atgcaggcgg aaattgcgca aatccgccaa
     8401 gacacgaaac gcacggccat tacattcggc gcactgggcg gcggcgtgat tacggtcggc
     8461 tgggaattgc ttaaagcgaa aatgggactg taattatggc tcacccgcaa gaaatccgtg
     8521 aaaagttacg tcggctctat gtgagcggcg agcaaacttt ggaaacggcg gccttgatgt
     8581 gcgaaatctc gcagaccact gcgcgtgcgt ggaaacgtgc ggataaggaa aaaggcgacg
     8641 actgggataa gatgcgcgcc gcctacactt tggccggcgg cggtattgag gacttgagcc
     8701 gtgcgatgtt ggccggtttt atggtgcagt acaacagcac gatgacgatg ctgcaggatt
     8761 cgagtaccga agatttgccc ccgtccgacc gcgccaagct gttggccagc ctggccgatg
     8821 cgtttacgaa aaccgtatcg gccaatgcgc gtgtgatgcc ggaaacgtca aaactggcga
     8881 cggctttgga attgattgag ttcttgatgg cgtttgtgca agaaaaacat cccaaacatt
     8941 tgcctgcctt tgtggaggta ttggagccgt ttggggcgga agtggagaag aagtttggtt
     9001 aatagccgtc tatcaatctc ccataacgtg atcctcctac tgaaaatact tcaacatctg
     9061 actttaaacg ctctcccaat attatggttt ggcaacggga acaggaatat ttatgaaaag
     9121 caccttcata cccattgaat tgaagaatcg atttgacacc ctgctcgtaa cattggggac
     9181 aaatatcatg aattggctcg ctgttcgcca tctctgtttt caatcgatag acgaaagaac
     9241 caggagcaag ttcatacaat tggtagcgtt gcttctctgt agaccaattt tttactgcca
     9301 ccagttcttt ttccaactca tctatacggc taattgccgc cgtataagaa gattgggctt
     9361 caaataaatc agtttgaata ccagcaatga tttgattaag ttcgatggtt ttttgcgcta
     9421 cctctgcctc tgttttgagc tggttaaacc cttttaaaag tgatcctgct tcttttaggc
     9481 ctataaatgc gttagaaatg aatccagtca ttataaatac tcatgaaaac aaaagaattc
     9541 ctcaaatccc ttgccgaact ggccgccagt ttgcgccaag tcatcgaagc ggaagtggac
     9601 ggctttgatg cgtcgcccaa ggctattgct gcacgccgtg ccaaggtgtt tgacccggta
     9661 ggcggttacg agtatttcgt caacacctac tttccgcatt atatccgctc ccctgaaaaa
     9721 tccgaactgc atgcgttttt attcagccgt ctgccggaga ttatccgctc ccccaaaggg
     9781 gaaaatgagg cggtgggtgc gccgcgtggc gagggtaagt cgacgcaggt tactcagttg
     9841 tttacgctgt ggtgtattgt gacaggccaa aaacattatg ccgttattgt gatggacagc
     9901 atagatcagg catacccgat gctcgaagcc atcaaggcgg aattggaatt taatccgcgc
     9961 ttgaaaaccg actttccgga agtatgcggg cagggccgcg tatggcaggc cggtacgatt
    10021 gtgacggcca atgacgttaa agtccaagtg gccggtagcg gtaaaaagct gcgcggtttg
    10081 cgtcacggcc cttaccgtcc tgacctgacc atattggacg atattgagaa tgacgagcaa
    10141 gtccgcaacc ccgaacagcg cgacaagctc aatgcgtggc tgactaagac tgtattgcct
    10201 ttgggcggtg tcggccagaa atacgatgtg atatatatcg gcacgatttt gcattacgac
    10261 agcgtactta accgcacttt gaataacccg ttttggcacg gtattaagtt taaggcgatg
    10321 aaacgctggc ccgaccgcat ggatttgtgg gacagatggg aggaactttt ccgaaacgac
    10381 ggcgagactg tggccgaggc gttttatctc gccaataaag acgagatgga gcgcggtgcg
    10441 caaacaagct gggcggctcg cggcgtactc gcgctgatga aaatccgtgc gcgtgacggt
    10501 catgcgacat ttgacagcga atatcaaaac gacccggtca gcggcgaaga tgcgccgttt
    10561 gccaagtcga tgaagttttg gaacgacctg ccgtccgatt tggtgtattt cggtgcgctc
    10621 gacccgtcgc tgggtaaagc gggggcgagc cgtgacccgt cggcgattat cattggcggt
    10681 tatcaacgtg aaaccggcaa actgtatgtc gtggaagctc agattaaaaa acgtctccct
    10741 gatttgatta ttgaggacgt tatcagattg caccgtcaat atcgttgcaa actgtggttt
    10801 gttgagacgg ttcagtttca ggaatttttg aaagatgagc tggtcaagcg cagcgcggcg
    10861 cgtggaatac ctgtcccggc gcgggcggtc aagccggtat cggacaagct cttgcggatt
    10921 gagactttac agcctcacat ggcgaacggt ttgattctgt tgaatgagag ccaacagacg
    10981 ctgatacagc agttccgcca ttttccaaag gctgatcatg atgatggtcc tgatgcagtg
    11041 catatgctct ggtcgggggc ggtggccaat tgtgtgccga tagaatggca aagccctacc
    11101 gataacgatt ttgatgacga gataaaaagt aaatggagcc gataatggca aaaaagaaca
    11161 ataaaactaa aatccaaaag cccgaagctg cattgcagac ggacgtggct caaattacgg
    11221 cgaccggtcg ggttatcgcc gagcatccgt ccaattttat tacaccgcaa aagatgcggg
    11281 ccctcttcga ggatgcggaa agcggcgaca tccgcgccca acacgagctt ttcgcggaca
    11341 ttgaggagcg cgacagcgac atcgcggcaa atatggggac gcgcaaacgc gcgctgctga
    11401 cgctcaactg gcgcgtcgcc ccgccgcgaa atgcgacgcc cgaagaagaa aagctgtccg
    11461 accaagccta cgaaatgatg gacagcctgc ctaccctcga agacctgatt atggatttga
    11521 tggacgcggt agggcacgga ttttctgcgt tggaggtcga gtgggtattt tcagacggcc
    11581 tttacctacc ccgaaacttt atccaccgcc cgcaaagctg gttcaaatgg gacaaagaca
    11641 acgggctgct gctgcgtacc cgcgaaaatc cggaaggcga agcgttgtgg ccgctgggct
    11701 gggtcgttca tacccaaaaa tcgcgcagcg tccagcaggc gcgcaacggg cttttccgca
    11761 cgctttcctg gctgtatatg ttcaaacact acgccgtcca cgattttgcc gagtttttgg
    11821 agctgtacgg catgcccatc cgtatcggca aatacggcgc gggcgcaacc aaagaggaaa
    11881 aaaacaccct gcttcgagcg gtggcggaaa tcggtcacaa cgcggcaggc atcatgccag
    11941 aaggtatgga aatcgagctg cacaacgctg caagcggtac gatggcaacc agcaatccgt
    12001 ttttgcagat ggccgactgg tgcgaaaaat cggcggcgcg gctgattttg gggcaaacgc
    12061 taaccagcgg tgcggacgga aaatccagca ccaacgcgct gggcaatatc cacaacgagg
    12121 tacgccgcga tttgctggtg tcggacgcaa aacaggtggc gcaaaccatc acaagccaaa
    12181 tcatcggacc gttcctgcaa atcaactatc ccaatgccga cccaaaccgc gtgccgaaat
    12241 ttgaatttga cacgcgcgag ccgaaagaca tcgcggtctt tgccgacgct atcccgaaac
    12301 tggtggatgt cggcgtacaa atccccgaaa gctgggtgcg cgacaaactg gtcattccag
    12361 atgtgcagga gggtgaggct gtgttggtgc ggcaggtacc ggacaatccg ataaacagaa
    12421 ctgcattggc ggctttatcc gcccacaccg taccatctaa ggctacgggc aggcatcagg
    12481 aaatattgga cggcgcgttg gatgacgcgc tggttgagcc cgatttcaat tctcagctca
    12541 acccgatggt gcgtcaggcg gttgccgcac ttaatgcttg caacagctac gaggaggcag
    12601 atgccgcact gaatgcgctt tatccgaatt tggacaacgc gaaactgcgt acctatatgc
    12661 agcaggcctt gtttatcagc gatattttgg gacaagacca tgcccgcgcc tgatttggga
    12721 tttgccttaa gtctgccgcc aaaaaaggca atcgagtggc tggaaagtaa aaaggttacg
    12781 gcggagagct accgcaatct gacagcctcc gaaattgcca aagtctatac gattgcccgc
    12841 atgaccgact tggatatgct caacgacatc aaaacttcga tggttgaatc ggcaaaaagt
    12901 ggacagtcgt ttgacgattg gcgaaaaggt atcttgaatc tgctcggcaa caagggctgg
    12961 ctgcatccga acgggcataa cggtaaggat atcatcgacc cagccaccgg cgaggtattc
    13021 ggttcgccgc ggaggttgga gacgatttac cgtaccaaca tgcaaactgc ctacaacgcc
    13081 ggtcaatatc aaggatatat ggcaaatatt gatgcacggc cttattggat gtatgacgcg
    13141 gtaggcgaca gccgcacccg tccggcgcat tcggcaatag acgggctggt gtaccgctac
    13201 gacgacccgt tttgggcaac gttttacccg cccaacggct acaactgccg ttgctcggtc
    13261 atcgcgctgt cggagcggga tgtggaacgc caggggcgga ttgtcgggca aagcacggcg
    13321 gacaatctgg tcgagaccca taaaatctac aacaaaaaag gcgatactta tctgaccctt
    13381 gcctataaag caccggatgg cagtctgtac acgaccgatc gaggatttga ttacaacgcc
    13441 ggacgaatga actaccgccc cgatttagac aagtacgacc gtgcgttggc gcatcaattt
    13501 gccaaagcgg aaatgggtgg tgcggatttt aaaaccagct ttaaacagct tgaaaaagag
    13561 ttttatgaag tcaagcaacg tttggatatt gatggcaagc ccgataaaga gcagaaaatc
    13621 aaaatccgaa atgcgctatc aagacagctt aaatttgctg cgggtgtatt gagcaaggaa
    13681 acgcaagaat tggcaggtat gacacgagcg acggtgtggc tgtctgatga tacattggtc
    13741 aaacaggtgg acagtcggga ggggcaatcg tttggaacag atttatatgc catgctcccc
    13801 gatctgattc atgagcccga atatattttt tatgacaaaa gcagaggaaa agaaagtttt
    13861 gttttgctcg gaaaaccgtt ggaggggaac gaaaaataca gattgatggc agttttgaaa
    13921 tatctgccac agtacgattc gatttttttg gattcgtttc gcagggcgga tgaaaaagaa
    13981 attgtgaaaa gtaagaaacg ctatgaaatg aaaaaaggct actaagtcgg actgcaatcc
    14041 cgacacactc tcgcttatgt aaaggatacc aatcccagtc cgaggactgc ataagggctg
    14101 cggagggcag acaaattcac cgctttttta gtagcctagg caggataata ccatgattga
    14161 tgtcaaaata gacaatatct ttgtcgtcct aaaccaaatc gagcggcttg gcaacgggat
    14221 cgaaaaccgc tacctgctga tgcgccgact gtccgaaacc atgcacacgg cggtcaagct
    14281 caatttccgc tacgcaggcc gtccgaaatg gttggggcta aaataccgcg acggcaagcc
    14341 gctttcggat tcgggtcgtc tgaaagacag tttttccaca ctgtcagaca acgatacagc
    14401 ccttgtcggt acgaatatcg tctatgccgc catccacaac ttcggcggta tggcggggcg
    14461 caaccgcaaa gttcggattc cgcaacggga atttttgacg ctgacggacg acgacaaaca
    14521 ggctttgatg gacgatgtgc aggattattt ttcgggtctg ataccgtgaa tttataaaac
    14581 cctcaaaaac gcgcttttta gcgcgttttt ttatgcgggt aatacaaacc cctgcccaag
    14641 atataaaaat caatcctaga ctcttctaaa aagcccctga aaacgattaa ttgtgtatcg
    14701 cgcggacagg ttttaaaaaa atggcgggag ggtttgaagc acgcctactc tttgttgttt
    14761 tttcaaatag gcaaaatgac cgtattgaga gaggtacaca tgtccaaaaa tgcacaaaaa
    14821 accctacttg ccgtgtgcag tttcgaggtg cagccaaaag acgggcgaat ccaactgctg
    14881 ccatatggcg aatttcgcgc agtagacggt cgtccgactg atgtccctgc gtggtatctg
    14941 accgaagaaa acggtcatga tgtcgcgttg ttggccaaca gctcgcgcaa tcagttggtt
    15001 gtcgattatg aacaccagac gctctacaaa gagaaaaacg gacaacctgc acctgccgcc
    15061 ggttggatgc gttggctgga gttcacgcct aaaggcatgt ttgccgaagt ggagtggacg
    15121 gacaaggcgg ctgcggcaat tgccgcaaaa gagtatcgct acatctctgc tgtgttttcc
    15181 tatgacacaa agggatatgt aagcaaaatt tttcacgccg cgctgacaaa tttccccgcg
    15241 ttggacggta tggacgaagt gctggcggca gcgtcggcgc aaattttaaa accggaaacg
    15301 gagcaaaacc ctatgaaaga gttgttacag caactgttcg gcctgcctga tgcgggcgaa
    15361 gaagaactga aggcggcatt gtccgcgctc gtggaagcca agccggaagg tgcggctttg
    15421 tccgccgatg tattcgcgca gctggcggaa aaagacagcc gcattgcggc tttgtcggcg
    15481 caaaacggca cgcccgacct gacgcgatac gcgccggtgt cgctggtgcg cgacctgcaa
    15541 aaccaagttg ccgccctgac cgccgagcgc gaagccgaca aaggcggcga actgattacc
    15601 gccgcactgt ctgctggcaa gctgctgccc gcacaaaaag aatgggcgga aggcgtgctg
    15661 aaacagccgg gtggctttgc ctttttgagc ggctttatcg aaaacgccca gccggtcgct
    15721 gcactggcag gctcgcaaac gggcggcaaa gcacccgacg aacgcgtcgc cgcactgact
    15781 gcggaagagg cagccgcagc aaaaatgctg ggcatgtccg gcgaagaatt tgtaaaaatc
    15841 aaagaaagcg aaggtaagta atggacaaat cagcgatttt gaccgcaatc acggcagcat
    15901 tccgcaaaga atttcaaacg ggggcctgca aaaccgcctg aaccgtttaa ccgccaaata
    15961 ggtgcagccg gtagcgcagg cagtgcgcct gttatccacg tcatcgctgt tgtcggtggc
    16021 aacggcatta atcgaggcgc atggcgaaga gatgaccgcg cccgatttga ttgaggttaa
    16081 ccgcgccatg cgccgccgta tgcaggccga gattgccgcc ttgcgggcgg tgcagacggc
    16141 tgctgccgag tctggtgggc tgacggccaa cgccgtgtat accgaggctt accaaacggc
    16201 agaatccctg cgcgcggcgg caggcagtct gaatgcgttg gttgcggcgg ccatcaacca
    16261 aaagccgccg ctgattgtgc gccaagcccc aatcgacggt acgatacacc aaatcgccca
    16321 cgagttttac ggcgatatag cccgcgcagc agagctggtg cgactcaatc cccatatcca
    16381 ccaccccgcg tttatcaagc gcggcacttt ggtcaacagc tatgcaaaat aattcatacg
    16441 gctatgccgt gtcggtgcgc gtgggcggta aagagcaccg ccactgggag cgctacgaca
    16501 tcgacagcga ctttttaatc cctgccgaca gcttcgattt tgtcatcggc aggttggggc
    16561 cggaggcggc catacccgat ttaagcggag agagctgcga ggtagtgata gacgggcaaa
    16621 tcgtgatgac gggcatcatc ggcagccagc gccacggcaa aagcaagggc agccgcgagt
    16681 tgagcttgag cgggcgtgat ttggccggtt ttttggtgga ttgctccgcg ccgcagctca
    16741 atgtaaaggg catgacggta ttggatgcag ccaaaaagct ggccgcgccg tggccgcaga
    16801 ttaaagcggt ggtgcttaag gccgaaaaca accccgcttt ggacaaaatc gacatcgagc
    16861 cgggcgaaac tgtatggcag gcattaaccc atattgccaa ctcggtcggg ctgcatccgt
    16921 ggctggagcc ggacggcacg ttggtggtgg gcggtgcgga ttacagcagc ccgccggtgg
    16981 cgacattgtg ttggagccgc accgacagcc gctgcaatat cgagcgcatg gacattgagt
    17041 gggataccga caaccgcttt tccgaggtta cttttttggc gcaatcgcac ggccgcagcg
    17101 gcgacagcgc caaacacgat ttaaagtggg tgtacaaaga cccgacgatg acgctgcacc
    17161 gccctaaaac ggtggtggtg tccgatgccg acaatttggc cgcattgcaa aagcaggcta
    17221 aaaagcagct ggccgactgg cggctggagg gatttacact cacgataacc gtgggcggcc
    17281 ataaaacccg cgacggcgta ttgtggcaac ctggccagcg tgtgcatgtg atcgacgacg
    17341 agcacggtat cgatgcggtg ttttttctga tggggcggcg gtttatgcta tcccgcatgg
    17401 atggcacgca aaccgagctg cggctcaaag aggacggtat ttggacaccc gacgcttacc
    17461 ccaaaaaggc cgaggcggcg cgcaagcgca aaggcaaacg caaaggcgtg agccataagg
    17521 gcaaaaaagg cggcaaaaaa caagcagaaa cggcggtgtt tgaatgagtt tgagtaaatt
    17581 ggcgaaaaaa acggcacaaa ctgctaaaaa tatcggcgaa accctgcgca cggcctttcg
    17641 gggaaaaatc acgctggtgg tgtcgtccga gccgatacag cgcgtgcagt tgagcggctt
    17701 ggccgacgaa accctgcaag accttgaaca tttgcaggaa tacggctttg ccagccatcc
    17761 gcccgacggc agcgaagcgg tagtgatacc gctgggcggc aatacttcgc acggtgtgat
    17821 tgtgtgcagc cagcacggca gctaccgcat caaaaacctt aagcccggcg agacggcgat
    17881 ttttaatcat gagggtgcaa aaatcgtgat taagcaaggc aaaatcattg aggccgattg
    17941 cgacgtgtac cgggttaact gcaaacaata cgaggttaat gcggccacgg atgccaaatt
    18001 taacgctccg ttggtggaga ccagtgcagt gttgacggcg caaggccaaa tcaacggcaa
    18061 cggcggcatg gccgtcgagg gcggcgacgg agccaccttt agcggcgatg ttaaccaaac
    18121 gggcggcagc tttaacaccg acggcgacgt ggtggccggc aatatatcgt tgcgccagca
    18181 cccgcatacc gacagcatcg gcggcaaaac cttaccggcg gaaccggcat agacaagcag
    18241 acctttggca gccttcgggc tgcttttttt gtgcgtgtgg gattgaagcc cgtgtactcc
    18301 gtgaggccgt ctgaaaacgg caaaatgcca acatggacaa agagctaaac cccagcatcg
    18361 gcgactatac cggccgcacc gtcgatacgc tgcaaaatgc cgtgtatatc cgcttgatga
    18421 caccgttggg cagctggtgg gcggataaaa cgctcggctc gctgctgcat ttgttgcagc
    18481 gcgaaaaaga cctgcaacgg gtcagcctgt tggccgagca atatgccgat gaggcactgc
    18541 aaccgattgt taagagcggg cgtgccgaca agattaccgt gcgcgcagag cagccgcacg
    18601 acggccgcct gatcctgcat atccgggtgg atacggcggc gggcgggttt gattaccgcc
    18661 acgaagtgcc cgtgatttaa agaggtttta aacgtgtttg aaacgccgac atttgagcaa
    18721 atccgcgagc gtatcctgcg cgataccaaa agcctgtggc cggatgccga tatcagcccc
    18781 gacagcgacc attatgtgca cgccagccgt ttggccagct gcgccgaagg gcaatatgcg
    18841 catcaaagct ggattgtgcg gcagattttc cctgataccg ccgaccgcga gtatttggag
    18901 cggcatgcct ccatgcgcgg cttgagccgc cgcaatccta ccacggccag cggcacgctg
    18961 accgtaagcg gtattgcgca atccatgctt tcagacgacc tgcaagtgcg tatcggccag
    19021 cgtttttacc gcactaccgc ccgcgccgtt atcggcagcg gcggcacggc ggaaataccg
    19081 gcaatcgccg acgagccggg cgcggccgcc aatgtgcgcg acggcgaggc gcaactgatg
    19141 gccgcccccg ccggtgtggc caccgaatgc cgccttaccg tacaaggcgg caccgaccga
    19201 gaaagcgatg cctcactgct ggcgcgtctg ttggaaatca tccgccgacc gcccgcaggc
    19261 ggcaaccgtt acgactataa aaactgggcg ttgagtgttg acggcgtaac cagcgcatat
    19321 gtttatccgc tgcgccgcgg cttgggtacg gtggatattg ccattacctc cgccgacggt
    19381 gtgccatcgg aagaaactgt gcgccgcgta caggcttata tcgacgagat gcgcccggta
    19441 acggcaaaaa atgcgctggt actcaagcca accgtaacgg cggtgcctgt taccgtgcaa
    19501 gtcaagctcg acggcatcga cttggacgag gccaagcgcc gcatacggac ggccctaaaa
    19561 gaatatttcg acaccctgat ccccggcgac ggcctgactg tgtcgcaaat cgaggcggct
    19621 atcagcaatg tggatggtgt gatcgaccgc cgtctgactg cgccgacggc caaccgtgcc
    19681 gccgatacgg ttaaccgcat cgagtggttt aaagcgggcg cgattaatgt aacggagatg
    19741 ccgtcatgag ctatcaagac atcttgcggg gcctgttgcc ccccgtgtcg tatgcccgca
    19801 atgccccgcg tgtgcgggcg caggcagaaa tagacggcgc agcgctggat gcggtggcgg
    19861 aatcggctca aagcgttgcc gatgccgtcg acccgcgcag cgccggccaa atgctggccg
    19921 attgggagcg cgtattaggt ttggacggta cgggcaaaaa ccgccagcgc cgtgtgttgg
    19981 ccgtcatggc caagctaaac gaaacaggcg gcttgagtat tccttatttt gtgcgtttgg
    20041 ccgaggcggc gggctatcaa atccaaatcg acgaaccgca gccgttccgc gccggtgtaa
    20101 accgcgccgg cgaccgtctt gcgccgcagg aaatcatgtg ggtgtggcac gttaacgtgc
    20161 gcggcggcaa caaccgcatt acccgattcc gcgccggtat ctcggcggcg ggcgacaggc
    20221 tgaccgatta cagcgatgcc gtgatcgaga gcctgttcaa ccgcctcaag cccgcccaca
    20281 ccgctatccg atttacctac cgataaggac taaaccatgc accccatcga aacccccgac
    20341 aagaccttcc acgacggcga cggcgtgtcc gaattgggca ccatcctgcc cgcgtggtgg
    20401 ctcaaccaag tgcaatccga gctgctggcc gtgctgactg cggccggtat ccagccggat
    20461 aagtcgcagc ctaatcaatt actggcagca ctaaataggc tggctgtagt taccaccggc
    20521 gaccaaacca tcaccggcca aaaaaccttt actgcccaaa cccaattcca aagcggcatc
    20581 catttatccg ccaaccagac gaactggaac ggcggccaca aagcctacat cggcgcggat
    20641 gccgacaacg cccacatcgt cttcggcgac gacaccctcc gcctgcacag cgcaaacaac
    20701 cgcatttcct acaacaacca cgacatcttc cacaaagcca acaaaccgcg ttttgccgaa
    20761 gacatcgaag gcaaaccgaa cacactgtcc ggatacggca tcggcaattt caaagtcgaa
    20821 acattccggg gcgatttgaa caccctcaaa acagacggca tctattccct gccgacggcg
    20881 gtcggcagct ccaacctgcc cgttgaaaac accgcctgcc atatccaagt catcgccggc
    20941 acgaaacacg gctggtgcag gcagttgggt tatcccgcct acacgtccga cgtgtacgaa
    21001 cgccaccaaa cgagcagcgc aaacgacaac tggtccgcat ggaaaaaact caattcggac
    21061 ggcatccccg tcggcgcgat cgtatccttt cccaaagccg tacaaaaccc cgcaggctat
    21121 ctcaaagcca acggcacgac ctttgcgcaa aacaccttcc ccgacctcta ccgcgccttg
    21181 ggcaacagca accgcctgcc cgatttaagc cgtaccgaca tcggcatcac cgcgtggttt
    21241 ccgtccgacc aaatcccgac cggctggctg gcgtttgacg acatccgcac gcgcgtaacc
    21301 gaaaccgctt accccgagct gtaccgtctg ctgaccggaa aatacggcag catccaaaac
    21361 gtcccgcagg cggaagaccg ctttatccgc aacgcgggca acggcttagc agtcggaacg
    21421 aagcaggaag acgaaatcaa acggcacgtc cacaaagtat tttcacactg gacaaaccac
    21481 acagacgcag ccgccgtcgg ttacgaagac cacaacgaaa ggcagagaag cgcgctcgta
    21541 tcgacttgga cggacggcga tttaaacgac aacggctttt taaccccgcg cctggacagc
    21601 aaaatggcga caggcggcgc agaaaaccgc cccaaagccc tggttttaaa actgtgcatc
    21661 aaagccgccg acaccttggg cgaagccgtg ttttggataa agtcccacgg cgaaaccatc
    21721 aacgccggcg cgctggacgc gggcacgctg gcgcaaaacc tgcaagacaa agccgaccgc
    21781 gcccacaccc acaccgccgc ccaaatccaa gggctggacg aaaaaatcag caccgccgtt
    21841 gccgcgcaat tcacacgcca aaccatcggc ggcgtggata ttgtcagatt ccccgacggc
    21901 acaatgatac agaccggcag ttacaggttt acaagaagcg gcggccccat cgaaaacgaa
    21961 gtcgtcttcc ccgtcgcctt tgccgacggc aacgtcaaat gcttcgtatc cgaacgccat
    22021 tcgggacgcg tcaccggcga tcgaaggcaa cacaactggc tgtttatccg cgcaaaaaac
    22081 cacgccgccg ccattattac caactggtac gaaggcagtt gcgactggat ggccatcggc
    22141 aaagccgcct cgggaaacgc cgccagctcc ccgataggcc ccgaaatacc tgaaaccaac
    22201 gaagaaccgc aaagagagag tggaagaaca tcaaccggac cccgaaaccg ccgccgccac
    22261 cgagacggcc tgctcgaggc actgcaagac tagcgggctg tagagatggg ttgtagagat
    22321 ggctgtagag atggctgtag agatgggctt cagcccgccg atccaagcaa tccgaccgaa
    22381 accggccgcg ccgccaatcc cccgaaacct atgccccgcc aatcctgcca ctcttcgtca
    22441 ttcccgccgc tttcgtcatt cccgcgaaag cgggaatcca gcccctaaac gcggcaggaa
    22501 tctatcggaa aaaacagaaa cccccgccac cgtcattccc gcgaaagcgg gaatctagac
    22561 cccaaacgcg gcaggaatct atcggaaaaa acagaaaccc ccgccgccgt cattcccgcg
    22621 aaagcgggaa tccagacccc gacgcaacag gaatctatcg gaaaaaacag aaacccccgc
    22681 cgccgtcatt cccgcgcagg cgggaatcca gaccccaaac gcggcaggaa tctatcggaa
    22741 aaaaccgacc ccccgccgcc gtcattcccg cgcaggcggg aatccagacc ccaaacgcgg
    22801 caggaatcta tcggaaacgg ctgaaaccga acggactgga ttcccgcctg cgcgggaatg
    22861 acggcggcag gggtttcggg attcccgcct tcgcgggaat gacggaaagt ggcgggaata
    22921 acgaaaggcg ggaatgaccg cgcaaaaagc cgctgccccc ttcggacggc accggcaaca
    22981 aaaaaccgca cggccgaaac cgcgcgggaa aggatagtcg ggcgcgcccg ataagcagcg
    23041 gccgcccccg ttatttcaat tgggcgacat attggcgcaa aacctcgttg atgcgcgtct
    23101 gccagccctt gccgccggcg cggaattttt cgaccacatc ggcggacagg cgtatggtaa
    23161 cgagttgttt cggggttttg cctgtgtttc gtttttgcat tacgcccttt tcttccaatt
    23221 gtttttgatg ggaaaaaagc acctgtgcca agtcttcggg cagggcttcg gcaatggggc
    23281 gggcaagcgc aaaatcttcg gcggcaagtt cccgcacttc gccgtcagcg tttgttaagg
    23341 attgacgttg catatttttt aacctctctt ttattcgctt tgcgaaaact gatgacacgg
    23401 atgccgtctt ttatcggcgt aaaacagaca atgtgcaggc gttgcgtatc gcctagataa
    23461 gcagcggcaa cataacgcgg ttcggggtaa tcaaagcgga catcgggcac aataacggcc
    23521 gttgtccagc gtatttgccc gactgattca aagggcaaat tccgctcttc gatattgcgt
    23581 tgattttttt cggagtcaaa ttcaatcttc attgcagctt gcagcgtatt ttgtcgttac
    23641 attataagcg gcaaaaaacc aaaatgtaaa tacaaaaaag gaaaccccga aatgaccatc
    23701 tatttcaaaa acggctttta cgacgacaca ttgggcagca tccccgaagg cgcggttgcc
    23761 gtccgcgccg aagaatacgc cgcccttttg gcaggacaga cgcagggcgg gcagattgcc
    23821 gcagattccg acggccgccc cgttttaacc ccgccgcgcc cgtccgaata ccacgaatgg
    23881 gacggcaaga aatgggaaat cggcgaagcc gctgccgccg cccgtttcgc cgaacaaaaa
    23941 accgccacgg cattccgcct cgcggcaaag gcggacgaac tcaaaaacag cctcttggcg
    24001 ggctatcccc aagtggaaat cgacagcttt tacaggcagg aaaaagaagc cctcgcgcgg
    24061 caggcggaca acaacgcccc gaccccgatg ctggcgcaaa tcgccgccgc aaggggcgtg
    24121 gaattggacg ttttgattga aaaagttgtc gaaaaatccg cccgcctggc cgttgccgcc
    24181 ggcgcgatta tcggaaagcg gcagcagctc gaagacaaat tgaacaccat cgaaaccgcg
    24241 ccaggattgg acgcgctgga aaaggaaatc gaagaatgga cgctaaacat cggctgaaaa
    24301 aatacgttta ccacctgttg gtagccatcg accaactgtt caacgcccta accggcggcg
    24361 cggcggacga aaccctctca agccgcacct atcgcggcgc gcggctcgcc caaaagccca
    24421 aaacccgctg gaaggtttta tacaccctga tcaacggcgt atttttcgac cgccaccact
    24481 gccgacaggc gtatatcagc gaactgaaag gcaggcagca cgacgcgcgg ttcaaccaaa
    24541 gccgcgccgc tggggaaaag gggacgcgat gaactacttc ggcatggtag agtttctgcg
    24601 cctgatggca atggtgcggc cgccatttgt cttcttctcc ggcacccgca gcgaactgcc
    24661 tgcttatctt gacctcgtgg cagaactgcg cctgaccgga tgggagcgtt ttgcaggcag
    24721 ccaaaccttg actgtgagca gcacatcaac agcaattcaa gctacgacga ccacctgatt
    24781 tataagttct gaccgcaagt agcgtactac ttttaaaggc ataagataat ccccgtttaa
    24841 cagaccatta aacggggata aatttgtgca aaagctaata caatttccta cgcttcggcg
    24901 gtgcaaaagc tgccgccaat tcgtgcaaaa gctgccgccg ccttacattc cagtgcaatg
    24961 ccgtctgaaa cttcgctaat ctcgggttgc tgcgtgctgt gtttttcttc cgtactcagc
    25021 aaaaagccgt acaaacgttc caatcgggcg cggtaggcag tgaatttgtt atagaacatc
    25081 cggaagaaag acagcgcgtt ttgcagtcgt gcgaaggctt ggacggtctg ctggatgtcg
    25141 ccgattttga tttggccggc aaacaggcgt ggggcttgca aaataatggg aaaaagcttg
    25201 atgccgttgg tgaacatatc gttaaagcca ctcaggcaga cgctttgccg cgcaatgcgc
    25261 cagcggttgc ggataatggc tttgaagcgg tcggcaagcc ggtcgtgttc gtgtttttcg
    25321 ccgctgtaaa acgccacgct ttcggcgtgg tcgcgcacgc ggatgaggga ataacggtag
    25381 tcgccgttga gcttttcgtt ttcatagttg taacgaatca atgggttgcc tatccacatg
    25441 gcgataaagg tcgccaaaat tacaaagata aagacgaacc aaacaatgcc gcgaggaatg
    25501 tcaaagccaa acacggtcag gatgcccgcc aagccccaca aaacaacggc aaactccagc
    25561 gaggtaacga ccgaattgac catgccgcgc acaaattcga tggtcgaggc gatgaaatcc
    25621 tgtgcgtcct gttggatacg ttggtcgatg ttgtccggcg cgtggcggcg catttgcagg
    25681 cggtagtagt ttttgtcggc aagccagcgt gctgtcagca cttcgttgag ccgctccgac
    25741 catttaatcg ccaagccttg attgaggaag tcgttgacga cgttgttaaa cgcccgtatc
    25801 agcaccacgc ccgcgttcat tgcggcaaac atccaaaatg ccgaagcatt tcaaatcctg
    25861 catcgagtcg taaagccctt tggacataaa ggtactcaac acattcagcc gcatttcggt
    25921 taacaccagc gtaatcatct ccgtaatcag cagcaagact ttgaccgcgc ttttcggtgt
    25981 cagacaaagg cgcaggatat aggcaaactc gcgcccgaaa cgcgttttcc gtgtcaaaaa
    26041 cagaatcacc gccgaagcgg cggaaactat caagagagtc tgtaaaagcc aaagcggtgt
    26101 ggaataaagc tcggtttgcc atttctgcat gggaaatttc ttacggtatc aatgccgtct
    26161 gaaaaagacg ggtacagttg attttttgat gaagtttggg gaagttttgc cggtcagggt
    26221 gcattgcgtg t
//
LOCUS       DAWGJV010000007        26059 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWGJV010000007 DAWGJV010000000
VERSION     DAWGJV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.007"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..319)
                     /locus_tag="WHQ94_000216"
     CDS             complement(<1..319)
                     /locus_tag="WHQ94_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ2127570.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
     gene            complement(366..1328)
                     /locus_tag="WHQ94_000217"
     CDS             complement(366..1328)
                     /locus_tag="WHQ94_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ2127571.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            complement(1355..1558)
                     /locus_tag="WHQ94_000218"
     CDS             complement(1355..1558)
                     /locus_tag="WHQ94_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127572.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPIHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            complement(1578..1994)
                     /locus_tag="WHQ94_000219"
     CDS             complement(1578..1994)
                     /locus_tag="WHQ94_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127573.1"
                     /translation="MKTSTVVFGGFFMADNRERIQIPVLENPGIREINHFFSVSNFEK
                     KAGVLVFRIIPEPKFGNTELTIYFEEGCYLPMIQTILEDGDIEVKNLKTENYSGNTME
                     IWGDFYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            complement(2021..2272)
                     /locus_tag="WHQ94_000220"
     CDS             complement(2021..2272)
                     /locus_tag="WHQ94_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ2127574.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            complement(2408..3463)
                     /gene="plsX"
                     /locus_tag="WHQ94_000221"
     CDS             complement(2408..3463)
                     /gene="plsX"
                     /locus_tag="WHQ94_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ2127575.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            complement(3551..4078)
                     /locus_tag="WHQ94_000222"
     CDS             complement(3551..4078)
                     /locus_tag="WHQ94_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ2127576.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            complement(4253..4432)
                     /gene="rpmF"
                     /locus_tag="WHQ94_000223"
     CDS             complement(4253..4432)
                     /gene="rpmF"
                     /locus_tag="WHQ94_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ2127577.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            complement(4466..4969)
                     /locus_tag="WHQ94_000224"
     CDS             complement(4466..4969)
                     /locus_tag="WHQ94_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ2127578.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            5117..5707
                     /gene="maf"
                     /locus_tag="WHQ94_000225"
     CDS             5117..5707
                     /gene="maf"
                     /locus_tag="WHQ94_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ2127579.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPKALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            5766..6479
                     /locus_tag="WHQ94_000226"
     CDS             5766..6479
                     /locus_tag="WHQ94_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ2127580.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6560..8197)
                     /gene="yidC"
                     /locus_tag="WHQ94_000227"
     CDS             complement(6560..8197)
                     /gene="yidC"
                     /locus_tag="WHQ94_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ2127581.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(8369..8590)
                     /gene="yidD"
                     /locus_tag="WHQ94_000228"
     CDS             complement(8369..8590)
                     /gene="yidD"
                     /locus_tag="WHQ94_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ2127582.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            complement(8656..9021)
                     /locus_tag="WHQ94_000229"
     CDS             complement(8656..9021)
                     /locus_tag="WHQ94_000229"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ2127583.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            complement(9024..9158)
                     /gene="rpmH"
                     /locus_tag="WHQ94_000230"
     CDS             complement(9024..9158)
                     /gene="rpmH"
                     /locus_tag="WHQ94_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ2127584.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            9451..11007
                     /gene="dnaA"
                     /locus_tag="WHQ94_000231"
     CDS             9451..11007
                     /gene="dnaA"
                     /locus_tag="WHQ94_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ2127585.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            11087..12190
                     /locus_tag="WHQ94_000232"
     CDS             11087..12190
                     /locus_tag="WHQ94_000232"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ2127586.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(12159..12428)
                     /locus_tag="WHQ94_000233"
     CDS             complement(12159..12428)
                     /locus_tag="WHQ94_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127587.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYPSDSRTKPRIRLHGSGHISNTGIVFRKTVKSASASQYI"
     gene            complement(12453..14510)
                     /gene="ppk1"
                     /locus_tag="WHQ94_000234"
     CDS             complement(12453..14510)
                     /gene="ppk1"
                     /locus_tag="WHQ94_000234"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ2127588.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDRNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALIIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIACETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPIAAPELKKRVIREGLEMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(14679..15122)
                     /locus_tag="WHQ94_000235"
     CDS             complement(14679..15122)
                     /locus_tag="WHQ94_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ2127589.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(15129..15644)
                     /locus_tag="WHQ94_000236"
     CDS             complement(15129..15644)
                     /locus_tag="WHQ94_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ2127590.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGCPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            15914..18544
                     /locus_tag="WHQ94_000237"
     CDS             15914..18544
                     /locus_tag="WHQ94_000237"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ2127591.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPSCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            18774..19565
                     /locus_tag="WHQ94_000238"
     CDS             18774..19565
                     /locus_tag="WHQ94_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2127592.1"
                     /translation="MNLFFDTKLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCSNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNKHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            19695..19826
                     /locus_tag="WHQ94_000239"
     CDS             19695..19826
                     /locus_tag="WHQ94_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ2127593.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            19849..20783
                     /locus_tag="WHQ94_000240"
                     /pseudo
     CDS             19849..20783
                     /locus_tag="WHQ94_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(21166..21477)
                     /locus_tag="WHQ94_000241"
     CDS             complement(21166..21477)
                     /locus_tag="WHQ94_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ2127594.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(21474..21842)
                     /locus_tag="WHQ94_000242"
     CDS             complement(21474..21842)
                     /locus_tag="WHQ94_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ2127595.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(22755..22840)
                     /locus_tag="WHQ94_000243"
     tRNA            complement(22755..22840)
                     /locus_tag="WHQ94_000243"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22804..22806),aa:Leu,seq:gag)
     gene            complement(22854..23204)
                     /gene="secG"
                     /locus_tag="WHQ94_000244"
     CDS             complement(22854..23204)
                     /gene="secG"
                     /locus_tag="WHQ94_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ2127596.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(23211..23984)
                     /locus_tag="WHQ94_000245"
     CDS             complement(23211..23984)
                     /locus_tag="WHQ94_000245"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ2127597.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            24168..24671
                     /locus_tag="WHQ94_000246"
     CDS             24168..24671
                     /locus_tag="WHQ94_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ2127598.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            24774..25430
                     /locus_tag="WHQ94_000247"
     CDS             24774..25430
                     /locus_tag="WHQ94_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ2127599.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYAAALLAKLAGRV
                     VSDDIDAEQQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            25510..25833
                     /locus_tag="WHQ94_000248"
     CDS             25510..25833
                     /locus_tag="WHQ94_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ2127600.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
BASE COUNT         5710 a         6307 c         7514 g         6528 t
ORIGIN      
        1 tcaggatgag ggtcgccagg acattgcccg aaacagtcag acggaacagc acaacaaaac
       61 gctttttcgc cgttttatgc ctgaatatcc tgctgcccaa gtatgcgccc gcccaaccgc
      121 cgaacaaggc aggcagcagc aggcggtgtt cgggaatgcg gcgttttccc cgcacggcac
      181 gccgtttgtc gatgccgtaa agtgcaaacg catagaggga caatattgcg taacacgcgc
      241 ccaatacggg gaggcgcagc gaaacggcag acagaaatgc cgcacacgcc atcagtttga
      301 agaaagcctg ccgcttcatg atatgggaaa tgccgtctga aagcctgttt cagacggcat
      361 cgggttcaat attgcaacag caccgcgccc cacgcgaaac cgccgccgat gccttcgagc
      421 agcaggtttt gaccgcgttt gatttgtccg ctgcggatgc ccgtatccaa agccagcgga
      481 atcgatgcgg cggatgtgtt gccgtggtct tggacggtca ggacgacttt gtccatactc
      541 aaacctaaat gtttcgcggt cgattcgata atgcggcggt ttgcctgatg cggcacgatc
      601 cagtcgattt gagcggcggt ataaccggct tcttcgataa cgtcatcggc gattttggac
      661 agcattttga cggcaaactt gaacacgccg ggaccgtcca tcgaaatgta cggcgaaccg
      721 gaaactttgc cgcaggcgat ttgcccgggg acgtttaaga gtttcagata attgccgtcg
      781 gccttgagtt tgctgtggat gatgcccggc gtgtccgacg cgcttaaaac caccgcgccc
      841 gcgccgtcgc cgaacaatac gcaggttgtg cggtcgttcc aatcgacgat gcggctgaag
      901 gtttccgcgc cgatgaccag cgcgtttttc gccatgccac ttttaatgta ggcgtttgcc
      961 gtggtcagcg cgtacataaa gccggcgcac accgcctgta cgtcaaacgc gggacagccg
     1021 ttggtgatgc ccaatttttg ctgcacgatg gtggcggtag aaggaaactg catatccggc
     1081 gttgccgtcg ccacgataat caaatcgatt tcgccgctgt ctaatccggc tgcatccagc
     1141 gcgcggcggg ctgcttcggc ggcaagatcg ctggttttct cattttcggc tgcaatatgg
     1201 cggaacttga tgcccgtgcg cgcggtaatc cactcgtcag aggtatctac cttttgggca
     1261 aggtcgtcat tgctgacgcg gttggcagga agatagctgc ccgtgccgga aatttttgca
     1321 tactgcatgg gaacggcctt tttagagagt tggttcagat tcggctattg taagcgaaaa
     1381 accgatgatt gcccaatacg gacagggtgc aaatgacgga aacagggatg ggggtgtggt
     1441 ttgggataca gatggttttc agatgcagat tgccgtggat aggcagggcg gggttgccga
     1501 tacgctgttt taagcgaagg aaatgccgtc tgaaaaccaa acatgcttca gacggcattt
     1561 tgattgttgg ttttccatca aattattggt gccggtgttt gtttttgtat cataaattca
     1621 gaaattatat tttgaattgt agaaatattt ttagagatat gctcaatagg gtagaagtct
     1681 ccccaaattt ccattgtatt tccactataa ttttctgttt taaggttttt gacttcaata
     1741 tccccatctt ctaaaattgt ttgaatcatt ggcaaataac atccttcttc aaaatagata
     1801 gttaattcgg tattgccaaa tttgggttca ggaataattc tgaaaacaag gacgccggct
     1861 tttttctcaa aatttgatac ggaaaaaaag tgattgattt ccctaatgcc aggattttcc
     1921 aaaacgggga tttggattcg ctccctgttg tctgccataa aaaatccgcc aaaaacgacc
     1981 gtcgatgttt tcattcttat caaatcattg gctttattgg ttaatcggac gtgctttcta
     2041 tctcaaattc cttgttgata aaagaaattt cccgggattc ttgttccgtc agccggacat
     2101 cgaaatcttc gatataagta cacaggattt caaaagccaa aaaacgttcc gaataatgaa
     2161 tataatccaa tgcggattca atcagacggg catcaagcct gccttcaagg gattttccga
     2221 acttcatcat ccgtttgtct agagctttca tccgtttttc taatgtcttc atttttcaaa
     2281 tattccttat tttacaggga agtatttggg aagtgccgga caggtgcatc gccgtccgcg
     2341 cccgcaggtt cggggcgggc ggatgccgtc tgaaaggggc gggggcgttc agacggcatc
     2401 gtgtgtgtta cagaccgccg acattttcgt tttggacggc tttggcagtt tcgagtgcgg
     2461 cgagttgttc ggcaacgcct tgttcgattt tggaaaggcc ggcggacttg gcttcgtggt
     2521 aggcttcttc gagtgcgtag cggaaaccgg tttcgtctgt gccgccgtgg cttttgatga
     2581 cgatgccgcg cagcccgagc aggatggccc cgttgaattt gcgcggatcg agtttgtttt
     2641 tcagcccttt gagggcgggt agggcggcaa cggcggcaag tttgttgaac aggttgcttt
     2701 ggaattcgcg gcggatggct ccgctcatga atttgaccgc gccttcgatg gttttgagca
     2761 tgacgttgcc gacaaagccg tcggcgacga cgacatctgc ttcgccgtac aggatgccgt
     2821 tgctttcgat gttgccgata aagttgagtt tgctgttttg cagcagtttg taggtttgtt
     2881 tgacggtgtc cgtacctttg atgtcttccg tgccgacgtt gaccagcccg acgcgcggct
     2941 gtcctttttg aggatggagt gcgtggacga gttcgctgcc gataacggca aattgggcga
     3001 gctgttcggg cgtgcagtcg acgttcgcgc caaggtcgag ggcgagggta acatggtcgg
     3061 tgtcggacgg gaggaatttg gcgatggcgg ggcgttcgat accgggtatg gtcttgagga
     3121 cgaaacgtgc ggttgccatg agtgcgcccg tgttgcctgc ggatacggcg gcttgggctt
     3181 tgccttcttt aacttggttg atggcaacgc gcatggagga gtcttttttg ttctttaggg
     3241 cggactgagg ggattcatcc atgccgacaa cttgggtggt atggcagatg tcgatgcgtt
     3301 ccatcggtgc gcctgccgcg gtcagggctt ggcgcagttg cgtttcgtcg ccggtcataa
     3361 tcaggcggac atcggggtgt gcttggagga atgcggttgc gccgggtacg gtaacggcaa
     3421 gtccttggtc gccgcccatg gcatctacgg caagtgtaat catagggttc tccggtaggt
     3481 tttcggtctg cggcaggggt cgtgcgggct tcagacggca tcggttcgga aaatatgccg
     3541 tctgaaaggg tcagcctcgg ccggcctcgg caaggcggtt gtgttcgtcg atgtcgagtg
     3601 cgtcccacgg atagttgatc caccagtctt ctacggtgat gccgctgaaa tagggaatgc
     3661 cttcggggat tttgcctgct ttggctttga ttttttcgtg cagtacggcg acgccgatcg
     3721 taccgaagtc ttctttgagc agctctgtca ggcaaaattc catggttacg cggctgtcgt
     3781 ccacttcatc gacaacaagg acgtttttgc cccgcagggc ttcgggaacg gggtcgagcc
     3841 attggacttt tttgacttct tctgtaacct gtccttcgtt gtcgctgtcg taataggcgg
     3901 tggttacggc ataaatcgga atttccagaa aacagcgcag catacgtgcc ggaataaagc
     3961 cgccgccgcc gatggcaatc atggcatcgt atttgatgtc ggcgttccgg attttttctg
     4021 ccaatgcttt gatgacgcgg tggatgtcat cgtaggtgta ccagattttc tgtttcatcg
     4081 gaatttcctt ggtggttggg gcttttggaa tattttcacg gggtattata cggaacgctt
     4141 tgcccctgtg tgttgacgta tggcgaaagt ttggacggat ggtgcgtcca atgcaaaaaa
     4201 agccagtaat tgcatggcaa tgttctggct ttttcagtca gtcgaatagg gattattcgc
     4261 ctttggcttt gaccactttg cggccgcggt acataccgtt gggggagatg tggtgcgggc
     4321 ggtgtacttc gccggttgtg ctgtcgacag acagtgcagg cgcggtcagc gcgtcgtgcg
     4381 aacggtgcat accgcgtttg gaaggggatt ttttgttttg ttgaacggcc atttcaagct
     4441 cctagataaa taaactgtgt cctaatcaat tgcttttcaa acctgccaaa acagcaaagg
     4501 ggttgggttt gtcccgattg acttcttcca gtgtcccatt gtcgccgcag tcttcgtgtc
     4561 gcggcgaaaa gggcagggac atcaggattt ggtcttctac caatgtgcgc acgtcgagtt
     4621 ctttttcaat cagtatgcct tcgagttctt cgtcggcaag catggattcg tccaaggact
     4681 cttcgttgga aaacaggacg atacggctgc tttcatcaag catgaacggc atgggtttga
     4741 tacatctctg gcaaatcagg ggcatatcgg ctttgacgtt caggtcgagg aacaggcgtt
     4801 gcagccggtc gcgaccgccg gtcagtgtaa acgatatttt ggtctgcctg tcggcgggat
     4861 aatcgtgcga actgacacgt tcatccaatt cttccagcag aaaactgcct tgcagattct
     4921 gcccttcggc ggcgaaaatt tccgggtcaa tcaaattagg gtctgacata aacggggtat
     4981 gatataattt agatgttcta acgtcaatat ttttaagaaa aatgttgcgg tacgccgaag
     5041 gcgggtatcc gtgtttcggc gcatggctgg attttaccgc cccttcgggc gtgttttcaa
     5101 cggtaaggag gatgggatgg gtttggaact gcctttgatt ttgggtacga gttcggtttt
     5161 ccgccgcgaa cagatggaaa ggctcggcat tgcctttcag gcggcgtccc ccgattttga
     5221 cgaaacgccg atgttggggg aatccgcccc tcagacggca ttgcgccttg ccgagggtaa
     5281 ggcgcggtcg ttgaccgggc gtttcccaaa ggcgttgatt gtcggtgcgg accaggtggc
     5341 gtggtgcgac ggcaggcagt ggggcaagcc gatgaacctt gctaatgcgc aaaagatgtt
     5401 gatgcatttg agcggcaggg agattgagtt ttacagcgcg gtcgttctgc taaatacggt
     5461 tacgggcagg atgcagcggc atatcgataa gacggtggtc gtgatgaggc agttggacga
     5521 gttgcacatc ctccgctatt tggagcgcga gcctgatgcg gtttattgtt cgtgtgcgct
     5581 gaagagtgag gatttgggtg cattgttgat tgaaaggatt gagagtaccg atccgaatgc
     5641 tttaatcggt ttgccggttt tccgtctggt cgattttttg aaaaatgagg gcgtggatgt
     5701 tttgtaactg cccgtttatg ccgtctgaag ggttcagacg gcatttttaa ccgatgagtg
     5761 agggaatgat gtctcctgtt ttgtatttga ttcctacgcc tttgggtacg cccgatacgc
     5821 cgtgcctgtt gccgcatgaa caacaggcga ttgtcgggct gacggatttt gtcgtggaag
     5881 cggaaaaaac ggcgcgcgcg catttgaaac atttgggcgt gacaatgcct atccgcgaac
     5941 tgaatttgca aacgctgaac gaacacacgg atttgaagac tttgctggaa ttgctgaaac
     6001 ctttgcaaga agggcgcagt atgggcattg tcagcgaggc gggttgcccg gctgttgccg
     6061 atccgggtgc gaatctggtg gctttggcgc ataaacacgg ttttgaagtg cgtccgctgg
     6121 tcgggccttc cagcctgctg ctggctttga tggcttcggg tgcgaacggg cagagttttg
     6181 cgtttaaggg ttatctgccg tctgaaaaaa acgagcggat tcagagtttg aaggctttgg
     6241 agcaacgttc gcgccagcag aatgaaacgc agctgtttat cgaaacgccg tatcgcaacg
     6301 atgtgttgtt ggcggatgcg gtggaaaacc tgcattctga aacgcgtttg tgtacggcta
     6361 cggatttgac gttgccgact caggaaatca tcagccagac ggttgtgcag tggcgaaaaa
     6421 gaaaagaaat gcccaatctg aaaaaacgcc cgacgatttt tgtgatgtat gcaggttgaa
     6481 gatttccgcc ccgatagggg gtgaacaata aaaatgccgt ctgaacaggt ttcagacggc
     6541 atttttcatg ctgcggcatt taggaaacga cttcgccttg ggcgcgttgt ttttcgatgc
     6601 tgcggttgat gtgccattgc tgggcgatgg tcaggaggtt gttgaccacc cagtacaata
     6661 ccagaccggc agggaagaag aagaacatga cggagaaaac caaaggcatg attttcatca
     6721 ttttcgcctg catcgggtcg gtcggcggcg ggttcagata ggtttgggcg aacatcgttg
     6781 ccgccataat gatgggcagg atgtagtagg ggtcggcgcg gctgaggtcg gtaatccaac
     6841 ccagccaagg tgcctggcgc aattctacgg aggcgaacaa tgcccaatac aatccgatga
     6901 agacggggat ttgcaacagc ataggcaggc agccgcccag cgggttgatt ttctcgtctg
     6961 tgtaaagctg catcatggct tgttgctgcg ccatacggtc gtcgccgtat ttctctttga
     7021 tggcttgcag tttgggcgcg gcggcacgca ttttcgccat cgaacggtaa gaggcgttgg
     7081 tcaatggata cagtacggct ttgacgatga tggttaaaac gataatcgcc cagccccagt
     7141 tgccgatgat gttgtgcagt tggttcaaaa gccaaaagag gggggaggcg aaccagtgta
     7201 ctttgccgta gtctttggcc agttgcaggt tgtcggcgat gtttgcgata acggatgtgg
     7261 tctgtgggcc ggcgtagagg ttgatggagg cttcggattt cgcaccgttt tggatagcgg
     7321 ctaaaggcac gctgacgctg gtgctgtaca gcttgtcgtt gcggcgtttg atgtcgatac
     7381 ggcagtcgcc agcggcgcaa acgctttgtc cgcctttggg ttggaggatc caggtggaca
     7441 tgaagtggtg ttcaatcatg ccgagccagc cggtcggggt tttgcggatg tattcggcct
     7501 cggatttgcc ggatttggca tcgtcgtcca agtcggagaa gctgactttt tggaagttgc
     7561 cttcaggggt ataaacaaca gggccgacgt aagagtgggt aaagtagcct tgaccctcgg
     7621 gttcgctgtg gtcgcggacg atgcggtagt ccgcgctcag gttggcggtt tgaccgctgc
     7681 cgttggcgat gtcgaagcgg acgttgacca gatagctgcc tttggtgaaa gtataaactt
     7741 tgtcgatttt cagaccgcgt gtttcaggtg cgctcaggcg gacttcaact ttgtcgcctt
     7801 ccaagctgta ctgttttttc ggtgcgctaa agccgatgcc ttttagaatg ttgttgccct
     7861 gcgcgtccaa aagttcggat tgggcgacgt aggtgtattc tttgccgtcg ccaaacagga
     7921 tgaacggttt attttcgtcg ccggttgctt tgtatttgag cagggtcagc cggcgcaggt
     7981 cgccgctttt ttcatcaatg acggcttgaa ccgtgtcggt cgttacggta atcggcgttg
     8041 cgggcgcgag cgcggcttcg gcggaagcgg ttacggcctg ttgttgtgcc gtctgttggg
     8101 gcgcggggac gggcttcgga gtggggaaca tcttttccca tccgatcata atcaccagtg
     8161 cgatggcgaa aaacgccgtg agtcttttaa aatccataag agtttcctga atggtcggaa
     8221 atgccgcagg ttgcgtgttc cgatagacgg ggaataaggg cgggcttcat acccgacggt
     8281 cgtgccgcca gcccgaaagt tcagacggca ttatatagaa accgatggga aataaaagga
     8341 aagcgtgcaa tttgaatatt gcgtcgggtc agggaacggg gtcgtgtccg tgtccgccga
     8401 aagggtggca gcgtgcaatg cgcttgatgg cgagccggcc gcctttgaat gcgccgtatt
     8461 ttttgaccgc ttcgaccgcg tattgcgaac aggtcggcgt ataacggcag cgcggcggaa
     8521 tcagcgggct gatgcaatat tggtagaacc gtatcagtcc cagcaggagt ttggacaata
     8581 ggaagttcat cgggatgttc cttagtatgg gaaacccgtt gccgtctgaa ccttgcctgc
     8641 agagtaccgt tctgatcatg cctgtttcct gcatccggtt gcggggttgc cgaacatgag
     8701 ttgtgccagt tccgcccttg cctgttttgc ggtagccctg tcgaatttac ggcggacgcg
     8761 cacgacgaaa tcctgcggcg gcagccggtt tttgttcaat ctgaaccagt cgcggatgac
     8821 gcgcttcata taatttcgtt cgttggcgcg tttggcggtt tttttgccga ccaccagacc
     8881 gatgcgggga tgatccagcc cgttgccgtt tgaacgcgaa acttgcagca ggtcgcggct
     8941 gcggcggttt ctgaatgcaa aaacggatga aaaatcatcc gttttcaaca agcggtactg
     9001 ccttccgaag cggtagtcca aaattacacc gccaggcgtt tgcggccttt ggcgcggcgt
     9061 gcagccaata ctgcgcggcc gccgcgtgtt ttggagcgca ccaggaagcc gtgggtgcgt
     9121 ttgcgtttgg taacggaagg ttgataagtg cgtttcatga tgtttcctaa aaatagaatg
     9181 ggtagataat taaaccgtga attacactcc aatttgccgc ttttgtcaat caatatagaa
     9241 actttgcagg aggattgccc gattgcctgc ggatttctgc cgcctttctg tggataaatt
     9301 ttgcacgggt tgtggataaa atatcggcga gtcggtataa ttcggttcgc tgcattttga
     9361 accgatgtgt gttcaacaga tttgttttct ttttgaaaat attatatttt ctttgttttc
     9421 gatttcattt ttaccgattc gagcttatcc atgacattag cagagttttg gccgctgtgc
     9481 ctccgccgtc ttcacgatat gttgcctcaa gggcagtttg cgcaatggat tgcgcccctt
     9541 accgtcggtg aggagggtgg cgtatgggtg gtgtacggca agaaccagtt tgcctgcaat
     9601 atgcttaaga gccagtttgc cgggaaaata gaagcggtga gggaagagtt ggctgctggc
     9661 cgttccgcct tcgtattcaa accaggagaa ggcgtgcgtt atgagatggc ggcggttgaa
     9721 ggtgctgtcg aacctgccga gccgtccttg cacgcggggt cggaggagat gcctgtgcag
     9781 gaggttctgt tggacgagct gccgtctgaa gagcctgtca aacccgctgc gtcgaaaacg
     9841 gcggcggata ttctggcgga acgtatgaaa aacctcccgc atgagccgcg ccatactgcc
     9901 gaacctgctt cccgtccgga atcggaggtg cttgcgaaag cgcggacgga tgcgcagcgc
     9961 gatgcggaag aggcacgtta cgagcagacc aatctgtcgc cggactatac gttcgacaca
    10021 ttggtcgaag gcaagggcaa ccgccttgcg gcggctgcgg cgcaggcgat tgcggaaagc
    10081 ccggggcaga gctacaaccc gtttttcctg tacggcagca cgggtttggg caaaacccac
    10141 ctggtacagg cggtcggcaa cgagcttttg aaaaaccgtc ccgatgccaa agtgcgctat
    10201 atgcattcgg acgactatat ccgcagcttt atgaaggcgg ttcgcaacaa tacctatgac
    10261 gtattcaagc agcaatacaa gcaatacgac ctgctgatta tcgacgatat tcagtttatt
    10321 aaaggaaaag accgtacgat ggaagagttt ttctatctgt acaaccattt ccacaacgag
    10381 aaaaaacagc tgatcctcac ttgcgatgtt ttacccgcca aaatcgaagg catggacgac
    10441 cgcctcaaat cccgtttttc atggggactg actttggaac tcgagccgcc cgaattggaa
    10501 atgcgcatcg ccattttgca gaaaaaggcg gaagcggcgg gcatcagtat tgaagacgaa
    10561 gccgcgctgt tcattgccaa tctgatccgt tccaacgtgc gcgagcttga aggcgcgttt
    10621 aaccgtgtcg gagcgagcag ccgctttatg aaccgtcccg tcatcgacat cgatttggcg
    10681 cgtaccgctt tgcaggacat tattgccgag aagcataagg tcattaccgc cgatatcatt
    10741 attgatgccg ttgccaaata ttaccgcatc aaaatcagcg acgtactcgg caaaaaacgc
    10801 acgcgcaaca ttgcccgtcc gcgccaggtg gcgatgagcc tgaccaaaga actgaccact
    10861 ttgagcctgc cgtctatcgg cgattcgttc ggtgggcgcg accatacgac cgttatgcac
    10921 ggcatcaggg cagtggcgaa actgcgcgaa gaagatccgg aattggctca ggattatgaa
    10981 aagctgctga ttctgattca aaactgatgg aaaacgtttt cagacggcat gatattgccc
    11041 atgccgtccg aagggtaaaa aatccaaccg atttaaagga gcgaacatgt tgattttaca
    11101 agccgaacgc gacagcctgc tcaagccgct gcaagccgtt accggcatcg tcgaacgccg
    11161 ccacaccctg cccatcctgt ccaacgtcct gattgaggga aaaggcggtc agaccaaact
    11221 cttggcaacc gatttggaaa tccaaatcga cactgcgggt cccgagggcg gcgcgggcga
    11281 cttccgcatc actaccaatg ccaagaaatt tcaggacatt ttgcgcgcgc tgcctgccgg
    11341 tgcgctggtg tcgctggatt gggacgacag ccgtctgacg ctgaaggcgg gcaaatcgcg
    11401 ttttgccctg cagaccctgc ctgccgccga ttttccgatg atgaatgtcg gcgaggacat
    11461 cagcgcgact ttctcgctgg agcaggagcg tttcaaaacc atgctgtcgc aagtgcagta
    11521 cagcatggcg gtgcaggaca tccgctatta tctcaacggt ctgctgatgc aggttgaggg
    11581 cagccagttg cgccttgtgg cgaccgacgg acaccgcctt gcctatgcag cctgcgcgat
    11641 tgatgcggat ttgccgcacg ccgaagtgat tttgccgcgc aaaacggtgc tggaactgtt
    11701 caaactgttg aacaaccccg acgatccgat tcaaatcgag ctgctggaca aacaggtgcg
    11761 tttccaatgc aacggcacga ccatcgtcag caaggtcatc gacggcaaat tccccgattt
    11821 caaccgcgtg attcctttgg acaacgacaa gattttcgtt ttgtcccgtg ccgaactttt
    11881 gggtgcgctg gaacgtgtgt ccattcttgc caacgaaaaa ttccgtggcg cgcgcctgtt
    11941 cctgcaatcc ggcctgttga gcgtcgtgtg cagcaacaac gagcaggaag aagcgcgcga
    12001 agaaatcgaa atcgcctatc agggtggcga actcgaagtc ggtttcaata tcggctatct
    12061 gatggacgtg ttgcgcaaca tccattccga cgatatgcag cttgccttcg gcgatgccaa
    12121 ccgctcgacg ctgtttaccg tgccgaacaa tccgaatttc aaatatattg tgatgccgat
    12181 gcggatttga cggttttccg gaacacgatg cccgtattgg agatatgccc cgaaccgtgc
    12241 agacggattc ggggttttgt tcggctgtcg gaagggtaat gccgtctgga atgcggcggc
    12301 ttggggttgg gagcgtatag gggaagtgct tgtgcaggaa tggcgaaggt gtgtttttga
    12361 ctccggtccg ccctgtttag attgctgtaa aaccatgccg tctgaaacgg aaaggtttca
    12421 gacggcatgg tttttgattg gggcggtgcg gttcagcctc cgagcagtgt ccacaaatcg
    12481 ttttgcagat cggcttcgct ttcgccctcg gcgggcgcgg cgcggatata gccgccgtcg
    12541 ggctgcatca gccatgcgtg ggtgttgtcg tccagtgcca tttccaaacc ttcgcggatg
    12601 acgcgttttt tgagttcggg cgcggcaatc ggcgcggcgg tttcgatgcg gcggaagaag
    12661 ttgcgcccca tccaatccgc gctggagata aaggtatcgt ccgcgccgtt gttgtggaag
    12721 caatacacgc gcgcgtgttc gagctgcctg ccgatgatgg agcggacgcg gatgttttcg
    12781 gacaagcctt ttacacccgg gcgcaaggtg cacataccgc gcacaatcaa atcgatttgt
    12841 acgcctgccg cgcttgcccg atacagggct tcgatgacgg tcggttcgat gagcgaattc
    12901 atcttggcgg taatccgcgc cggtttgccg gcttttgcgt gttcggtttc gcatgcgatg
    12961 cggtcgataa ccattttgtg cagggtaaac gggctttggt agagtttgtt cagccgcccg
    13021 ggtttgccca agcctgtgat ttccataaac aatgtgttca catcggcggt gatttgttcg
    13081 tcggcggtaa tgatgccgaa gtcggtgtag atgcgcgatg tgccttggtg gtagttaccc
    13141 gtgccgaggt gggcgtaacg cttgagtacg ccgtcttcgc ggcggatgat gagtgccatt
    13201 ttggcgtgta ctttgtagcc gaacacgccg tatacgacgt gcgcgcccgc ctcttcgagc
    13261 tgcttcgccc agttgacgtt gttggcttcg tcaaaacgcg ccatcagttc gacgacgacg
    13321 gttacttgtt tgccggcgag tgccgccttc atcagggcgc ggacgagttc ggagcgcgtg
    13381 ccggtgcggt aaatcgtcat tttgacggca agcacgtcgg ggtctgcagc ggcttcgcgc
    13441 atcatttcga caacggggtc gaacgattgg taggggtggt gcagcaggat gggcgattgg
    13501 cgcaccaaat cgaatatgga cgcggttttg cccaaggctt tcagtctgcc cggcgtgtgt
    13561 gtgggaaatt tcaaatcggg gcggttgact aggtcgggga cggcgttgag gcgcacgagg
    13621 ttgaccgggc ctttgacctg atagagttcg gcggcggtca gtttgaattg cgcgagcaga
    13681 aagtcgcgga tgtaggcggg acaggtgtcg gcgacttcga gccgcacgcc gtcgccgtat
    13741 tcgcggtcgt gcagttcgtt ttggatggcg gcgcggaggt tttgcaggtc ttcttcgtca
    13801 acggtcaagt cgctgtcgcg cgtcaggcgg aactggtggc agcctttgac gttcatgccc
    13861 gggaagagtt tgccgacgtg ggcgtggagg atggacgaca ggaagacgaa gccgtgtccg
    13921 ccgccacaca gttcggacgg caggggaacg acgcgcggca ggatgcgcgg tgcctgcaca
    13981 atcgccatcc ccgaaggtct gccgaatgcg tccgtgccgt cgagttcgac ggcgaagttg
    14041 agcgatttgt tcagcggtcg cgggaagggg tgggacgggt cgagtccgat gggggtcaga
    14101 atcggcagca attcgcggtc gaaatagctt tcgatccact ttttctgcgc gcccgtccag
    14161 ttgcggcggc ggtaaaaatg gatgccttct tgtgccagct cgggctgaag gacgttgttg
    14221 aacaggtcgt actggtgccg tatcagggag cgcgccgctt cggtaacgtc ggcgatggtt
    14281 tcagacggca ttttgccgtt gtccagcctg cgccgggggt gcagcttgtt ttcacgcttg
    14341 agccacgcca tgcggacttc aaaaaactcg tcaagattgg acgacacgat gcacagaaag
    14401 cgcaggcgtt ccaaaagggg gacgtttctg tcttccgcct gtgccaacac gcggcggttg
    14461 aatgccagca ggctcagttc gcggcagagg atgcggtttt gttcgggcat aaggttctcc
    14521 caaagatggt tgttgttcgg tcggagcggg caggcaatgc cgtctgaagg cttggcggca
    14581 tctgcgccgg tttcagacgg catgacgtgg cggacagcct gtttcaggca tcggggcaat
    14641 gtgaaaacag ataccggcgc gtgcggtatc cgtctgtttc aaatcacttc caacacaaaa
    14701 taatgacgca gtttttcgta caccgcatcg ctgacgttga tgcagccgtt ggtcataatc
    14761 ctgtcggaca cggacgggga ggcgatgcgt tcgttccgcc tttccgacgg tatctgattc
    14821 caaacgcggt gcagggcgaa aagaaaatca ccctcctgct tgaaaccgat gacttcgccg
    14881 ccgtatccag gcttgtcggt actgttcagc gtcagcccga acgtcccctt gggcgtggca
    14941 acaccgatga ggacggggtg gcactgacgg tcgtcagcga aacagagttc cgctttggat
    15001 gtatcgacga tgactttttt cttttggata taggcattga tcgcatccgg ttgcccctgt
    15061 gcatatacgg gggcaaccat tgacaggccg cataatatcc ctaaaaacag gcggaacggc
    15121 atgtcggatt attggcggat acgtttcggt tttgccggca cgatttcgcg gatgatgact
    15181 tgcggctcgg ctttgggtgc gggcggcgga caggcggcat ctttcgggaa gacggggttc
    15241 cagtagaagc tgcgggcaaa tttgtcttta tcgaaaatca ctttgtattg gcaggtagta
    15301 acatcttcta cgccggaagt gttttcaggg tcgataccta cgcccggggt atggaagtgg
    15361 aacaggtaat cccattcgcg cacgccgtac ataccttcgt cgtaatgcgg gcagcccagg
    15421 attttgtaga tgtcgtcttt ggtcaggccg gggcgcatct gatccagttc gtcataagtc
    15481 gggaatgtgc cgcgcttgtt gtcgagcgtt acggaatagg gtttcgggaa aaccggattg
    15541 tcggtcgtgc cgtcggcttt gacgttgctt ttggttgcgc aggcagacag aacgcccgcc
    15601 gccaatactg ccaagcccgg tttgacgatt tgtttgattt tcatatgcag aatcctcttt
    15661 tacccgatgc ccgtctgcct tttgttcgga agagccgcat caggagagtt ttaaacattt
    15721 gattttggtt tgtaatatta gcataaaaaa cgtgcgtatc aataaacgcg gtgtatttgt
    15781 acggcatacg gaataatgcg cgtgcgaatt tacgcatcct gccggcaatc tgccgattcg
    15841 ccgcttccgg caacctgtta taattgatgc cgactttatt tttccaacgt ttttaagcga
    15901 aaacagaatg actatgcaag aacaatatca acccgccgcc atcgagcctg cggcgcagaa
    15961 aaaatgggac gacgcccgta ttttcaacgt ctccgaagac gcttccaaac ccaaatatta
    16021 ttgcctctcc atgttcccct atcccagcgg caagctgcat atggggcatg tgcgcaacta
    16081 caccatcggc gatgtattga gccgcttcaa acttttaaac ggcttcaacg tcatgcagcc
    16141 tatgggttgg gacgcgttcg gcatgccggc ggaaaacgcg gcgatgaaaa acaacgtcgc
    16201 ccctgccgct tggacgtacg ataacatcga atacatgaaa acccagctca aaagcctggg
    16261 ttttgcgatt gactgggcgc gcgaaaccgc aacctgcaaa cccgaatact accgctggga
    16321 acaatggctg tttaccaagc tgtttgaaaa aggcatcgtt tatcgcaaaa acggcacggt
    16381 aaactgggac ccggtcgacc aaaccgtcct tgccaacgag caagttatcg acggacgcgg
    16441 ttggcgttcg ggcgcgttga tcgaaaaacg cgaaatcccg atgtattact tcaaaatcac
    16501 cgattacgcc gaagagctgc tcaacgactt ggacaagctg gaacactggc ctgaacaagt
    16561 caaaaccatg cagcgcaact ggatcggcaa atcgcgcggc atgaccgtgc gcttcgccgt
    16621 ttccgacgac agcaaacagg gcttggaagg cgattacgcg aaattcctgc aagtttatac
    16681 cacccgcccc gacacgctga tgggcgcgac ttatgtcgcc gttgccgccg agcatccgct
    16741 ggcaaccgcc gcagccgccg acaaacctga attgcaggca tttatcgccg agtgcaaagc
    16801 cggctcggtt gccgaagccg atatggcgac gatggagaaa aaaggcgtgc cgaccggccg
    16861 ctacgtcgtc aacccgctca acggcgacaa gctggaagtg tggattgcca actatgtatt
    16921 gtggggctac ggtgacggcg cggtcatggc ggttccggcg cacgacgaac gcgatttcga
    16981 gttcgccgcc aaatacagcc tgccgaaaaa acaagtcatt gccgtcggcg acaacgcatt
    17041 cgacgcaaac cgatggcaag agtggtacgg cgacaaagaa aacggcgtat tggtcaacag
    17101 cggcgacttg gacggcttgg attttcagac ggcattcgat gccgttgccg ccaagttgca
    17161 aagccaaggc gcgggcgaac cgaaaaccca ataccgcctg cgcgactggg gcatttcgcg
    17221 ccaacgctac tggggctgcc cgattcccat cgtccattgc gaacaatgcg gcgacgtacc
    17281 cgtcccagcc gaccaactgc ccgtcgtttt gcctgaaaac gtcgtacccg acggcatggg
    17341 ttcgcctttg gcaaaaatgc ccgagtttta cgaaaccgcc tgcccatcat gcggcggcgc
    17401 ggcgaaacgc gaaaccgaca ccatggacac cttcatggaa tcgagctggt acttcttccg
    17461 ctacatgtcg cccaagtttt cagacggcat ggtatcggct gaagccgcaa aatactgggg
    17521 cgcggtcgac caatacatcg gcggcatcga acacgcgatt ttgcacctct tgtacgcgcg
    17581 tttcttcacc aaactgatgc gcgacgaagg tctagtcaat gttgacgaac cgtttgaacg
    17641 cctgctgacg caaggcatgg tcgtttgcga aacctactac cgcgaaaacg acaaaggcgg
    17701 caaagattgg atcaaccccg ccgatgtcga gctgacgttc gacgacaaag gccgccccat
    17761 ttccgccgtc ctcaaagccg acgggctgcc cgtcgtcatc agcggcacgg aaaaaatgtc
    17821 caagtccaaa aacaacggcg tcgatccgca agaactgatt aacgcctacg gcgcggacac
    17881 cgcccgcctg tttatgatgt tcgccgcacc gcccgaacag tccctcgaat ggagcgacag
    17941 cggcgtcgaa ggcgcacacc gcttcctgcg ccgcctgtgg cgtaccgttt acgaatactt
    18001 gaagcaaggc gaagcggtca aagcgttcgc aggcagccaa gacggtttgt ctaaagaact
    18061 caaagacctg cgccacaaac tgcacgcgac cactgccaaa gtcagcgacg actacggccg
    18121 ccgccagcag ttcaacaccg ccatcgccgc cgtgatggaa ttgctcaacc aatacgacaa
    18181 aaccgacacc ggcggcgaac aaggacgcgc cgtcgcccaa gaagtattgg aaaccgccgt
    18241 acgcctgttg tggcccatcg tgccgcacat ctgcgaaacc ctgtggagcg aattgaacgg
    18301 cgcgaaactg tgggaagcag gctggccgac agtcgacgaa gccgccttgg tcaaatccga
    18361 aatcgaagtg atggttcaag tcaacggcaa actgcgcggc aaaatcaccg tcgccgccga
    18421 cgcctccaaa gccgacctcg aagccgccgc ccttgccact gaaggcgcgg tgaaattcat
    18481 ggaaggcaag cccgcgaaga aaatcatcgt cgtaccgggc agactggtga acatcgtcgt
    18541 ctaaaccgct cttaaggttt agccacacgg ataaaggccg tctgaaactt ggaaacaggg
    18601 tttcagacgg cctttttaag gcagattggg ttgtctgcat tacagacctc aaatatagtg
    18661 gattaacttt aaaccggtac gacgttgccc cgccttgccc tgctgtctgc ggcttcgtcg
    18721 ccttgtcctg atttttgtta atccactaaa actgaaatct caaaatccaa aacatgaatt
    18781 tatttttcga taccaaattg ggaaagcaac aaaataaagc aacccataaa atccgtgtaa
    18841 tgagtgaggc ttggctggaa aaaaacggct actgcccctg ttgcggaagc aagccgatgc
    18901 agagatttgc caataacaaa cctgttgcag acctcttttg ctcaaattgc cacgagcaat
    18961 atgaattaaa gagtaaaaat caaaaaacca taggtaacag tgtgcctgac ggtgcatatc
    19021 gcactatgtt ggagcgcatc cggtcagata ccaaccccaa ctttttcttt cttgcatata
    19081 aaaaagcgga ttactctata cggcaattgg tgcttgtacc caaacatttc atcacgccgg
    19141 acatgattat tcccagaaat aaaggcatta aaaaccggcc gaaccacatt atgtgttcca
    19201 tcaatctcgc ccctttgcct gaaagcggaa aaatattctt aatagacgat tctcgcatta
    19261 tcgaacccga aaccgttctg aaaaaatggc aatccaatct gtttttacgc aaccaaaatg
    19321 cggagcgcaa aggctggctt ttggctgtta tgaaatgtat cgaccaactc cccgaagaat
    19381 tcacattgtc gcaaatgtat gaatttgaaa acaaactatc catccaattt ccccaaaaca
    19441 agcatattaa agacaaaatc cgccaacagt tgcaaatttt gcgcgaccaa aatacgatcg
    19501 aattcattgg tcgcggactt tacaaaaaaa tcgacaaatt gcccccgact tccaaggcgt
    19561 tttaatttca aatcatgata ctgaattcac aagggatatt tattcttctg ctattctgaa
    19621 aacagttgaa gtacagcgca atattgccgc aaaaggcagc agccgtaaaa aagtcgggga
    19681 attattggca atttatgcgt aatttcgaca aattggctga agaagctgcc gaacacgccg
    19741 ctgaaggcgc ggtgaaattc atagacggca agcctgccaa gaaaatcatc gccgtaccgg
    19801 gcagactggc gaacatcgtg gtgtaaatct ttttaaaaac atcaaacaat gttaagatgc
    19861 cgtccgaaat ccgttttaga ttcagacggc attttttaaa tttaattttt taaggggtaa
    19921 gcctaatatg aaatttcctt ccgtccttcc ttccttcctt taaacggata ctctgcctgt
    19981 cggcagtaat ctcggtattg ggggcttgta cggtcgttgc tgatgtttac ggtcaggatt
    20041 ccgccacaat gaacgctgcg gctgccaaag attatatgaa aacggttgag ttgaacaagt
    20101 ctgccggcaa tgtcgatacc acatccagaa cagcccgcag ggtgcaggca gtatttcgac
    20161 gtatgctgcc ttatgccgat gcggcaaata ataccggcca taggtttgac tggaaaatga
    20221 cggttttcaa aaacgatgag ctgaacgcgt gggcgatgcc cggtggaaaa atggcgtttt
    20281 atacggggat agtcgacaaa ctcaagctga ccgatgacga aattgccgcc attatggggc
    20341 atgaaatgac gcacgccctg catgaacacg gtaaaaataa ggtcgggcag caaatcttga
    20401 ccaatacggc ggcgcagata ggcacgcaga ttatattaga caaaaaatcg gatactaatc
    20461 cggaattggt cggattgggt atggatattt tggggacgta cggtcttacc ttgccttata
    20521 gccgcagctt ggaagaagaa gccgatgagg ggggaatgat gttgatggcg caggcaggct
    20581 atcatccggc ggccgctgtc agggtttggg aaaaaatgaa tcaggaaaac gaccaaaacg
    20641 gctttattta tgctattacc tctactcatc cgacaaacaa tgcccgtata gaaaatctaa
    20701 aacggttgtt gccgaccgtt atgccggttt atgagcaaag tgtcagaaat aaggggcgcg
    20761 ttaataaaaa acgtcggcgt taagggaagg ggatcgagaa tttgagccat tgtttcaaaa
    20821 tgccgtctga aatcgtttga gattcagact gcatttaaac agttctgtgc cccacccgtt
    20881 ttgccttcaa gcccgcgccg cttaaatcca atccccccct gcaacgggcg caaaaaatcc
    20941 ccgtacctcg cggtcggtga tgtttcgggt ttcgcgcccg tctgcgccgt gccttgctgt
    21001 cgggctatgt cgtctgcacc gccgccgttt cggcttggtt tgatgcggtc gggcatttgt
    21061 gcggctacgc cctgatattc ggttcggcgg tcttgcctgt ttcgtcaggc atattcagca
    21121 gcgcgtctat tgtgaacttg ctgattttgc cgtttgccaa gtcgtctacg caatggcttc
    21181 gatgcccttg tttttgacga tggtcagcag cttcaacgcc gcgccgctgg gctttttaac
    21241 gccccgctcc caagccgaaa cgtgattttt tcccacgttg agatagatgg cgaaagcggc
    21301 ttgcgatagt gcctccttct ccctgattgc cttgatgtct ccgccgctca acggtttgat
    21361 ttcggtcagg caggacttgt caaagccgcg catagtcttt ttgtcgattg cgccgatgtt
    21421 gtaagcccct tccatcattt catgaatggc ggctaatgcc tcgcttttgt atttcatgat
    21481 tctatctccg tgataatgcc gtcttctttc aaagccgcca gctctgcccg cgtgtatttc
    21541 agataatatg cggcggcttt tcggtaaacg tccagttctt tatccgaaat gttttctctg
    21601 tcgttcttgg caaaggcgta aacgaaaaat gccctatctg cctgtttgaa caatatcagg
    21661 ctgcgatagc cgccgcttct gccttgtcct tgccttgcta tgcgctgctt aatcacaccg
    21721 ccgcctaaat ctgcgtctat cagcccgtta tccgcccgct ctaccgcttc cagcagctcg
    21781 gaatcactga ttttatgttt cttggcaaat ttcactatcc attggttttt gaatatccgc
    21841 attgtgtagc cgttgattag tgatacttgg aattatacca gagacagaca acacggcaag
    21901 ctgccttttt tatgccccac aatttctccg tttatgccgc ctgaaacact ggcggctaac
    21961 ctgattattt tttaatcaat cgcttttaat ggctcaaatt gcgtttttag cgcgtttcgc
    22021 tgtcttccta ttccccccgt atataaccaa gcggtttaac cgccattcct gctcaaaata
    22081 ggcgcggttc cgtgcgtttt ggcttttcat tccgctacgc tcccactgcc aagacggtga
    22141 aaaaccggaa aaccccaaaa gttgttaccg attcgttacc atttgttacc gcatttgtta
    22201 ccaatttttt atctttttct tattttatag tgaattaaat ttaaaccggt acggcgttgg
    22261 ctcgccttgc cgtactattt gtactgtctg cggctcgccg ccttgtcctg atttaaattt
    22321 aattcactat atttcatatg cttatttatc taacttttcc ccggtaacaa ggtaacaact
    22381 aagggtatcg cttcctagta taggtttttt ccaaaaaccc aaaaaaaacc gccctgcatc
    22441 ttggacggtt tcccccttgt tccctacgtc ttggcagtat tccccgcaag ctcttttccg
    22501 cttggcattc ctgatttggc ggtcgttcag ctttattttt gcggtatttt ttcgaggtat
    22561 ttttcagata ccgcaaaaga taccgtaaat tttaggttac ttcaattagg gcggattgga
    22621 cgggattgca ctgtcaggaa gggggacggc aggataacaa tcagcctgaa atccttgtat
    22681 tgattggaat tggttgacag cgttggatgg gattgaacaa aaaacgcctg aaattttcag
    22741 gcgtttttgt gtgttggtgc cgacagcgag atttgaactc gcacagccta cggccactac
    22801 cccctcaaga tagcgtgtct accaatttca ccatgtcggc atttgaaaaa ctgttatttc
    22861 tgctgctgag gaacaggggc agaaggttcg gtattgctta cgggtttggg tgcttgctga
    22921 gtttgttgta cgttgctgaa gtccaaaccg tgttttgtcg tgtgggtgtg aatatacacc
    22981 atagccatgc aggttgcaaa gaaaaatgtt gctgcaacgg cggtcgagcg gctgaggaag
    23041 ttagcgttgc cggcagagcc gaatacgcct tgcgcgctgc cgcttcccga tccgaaagtc
    23101 gcgccggcat ccgcgccttt gccgtgttgg agcaatacta acacgatgac ggccaaagcg
    23161 gaaattatat taacaatcca aattagggtt ttgaaggctt ccatattttt ctacgcattt
    23221 tgtgcggcac tgatgatggc ggtaaaggag tcgtacgaca atgacgcgcc gccgacgagt
    23281 gcgccgtcca cataaggtac tgcgaagatg tcggccgcgt tgtccgcttt cacacttccg
    23341 ccgtaaagga cgcggatttt aacatcgctt ccgcacaaag acaagatttc tttgtagatg
    23401 aatgcgtgca tatcggcaat ctgttcgacg gtggcgactt tgccggtgcc gatcgcccag
    23461 acgggttcgt aggcgacggc gatgtttttg gtatccagcc cttgcaggat ggaaagctga
    23521 tgggcgatga cttcgtgttc tttgccggct tcgcgctctt cgaggctttc gccgacgcac
    23581 aataacggga tgagtccgac gttgaggacg ttttccattt tgcggcgttg gatttcgttt
    23641 ttttcgccga aataaaggct gcgttcggag tgtccgatga ggacgatgtc tgtgccggtg
    23701 tcggcgagca tttcggcgga cacttcgccg gtgtacgcgc cgttgtcggg gaagcggctc
    23761 acgtcttggg cgcaggtgag gatgcggttg tttaagacga tttgcatggc gttgtgcagt
    23821 tgcagcaggt aaacggtcgg ggcggcgagt ccgatgagga cgcgttcggc ggtggggagg
    23881 atgcggaagc ggtgcatgag cgaattgttg ttctggagcc ggccgttcat tttccagttg
    23941 ccgatgaccc atttttgatc ccacattccg atttggtgat acatcttttt tgctccgtgt
    24001 cgtgtttttt ctgtctgccg cgtgtggcgc gttgcaatgt gaagtttagt ggatatgcgg
    24061 cgggttcgca acttggggcg ggcggctgcg ggggcggttt ggaatgttgt ttcgggcagg
    24121 ttgttttata atggccgcct gatatgtatg caactatagg agatgtgatg cacgcgcttc
    24181 atttttcggc ttcggacaag gccgcgcttt atcgggaggt gttgccgcag attgagtctg
    24241 tggtggcgga cgagacggat tgggtggcga atttggcgaa cacggcggcg gttttgaagg
    24301 aggcgttcgg ctggttttgg gtgggttttt atttggtcga tacgcgttcg gacgaattgg
    24361 ttttggcacc gtttcaggga cctttggcgt gtacgcggat tccgttcgga cgcggggtgt
    24421 gcggtcaggc ttgggcgaag ggcgggacgg tggtcgtcgg ggatgtggac gcgcatcccg
    24481 accatattgc ctgttcgtct ttgtcgcgtt cggagattgt cgtgccgctg ttttcagacg
    24541 gccgctgtat cggcgtgttg gacgcggaca gcgaacattt ggcgcagttt gatgagacag
    24601 atgctttgta tttgggcgaa ctggcgaaga ttttggagaa gcggtttgag gcttcgcgtc
    24661 aggcggtttg agactggcaa aacgagctgt cggcgcgtgc cgaagttggc gcggcgggag
    24721 tgtggtttta taatgcctgc cattgttgga acaattattt gacggagcac taaatggatt
    24781 ttgaaaaagc gcggttcaat atggtcgaac agcaaatccg tccttgggat gtattggatt
    24841 ttgacgtttt ggacgctttg gcggagattc cgcgcgagct ttttgtcgat gaggatttgc
    24901 agggtttggc gtatgcggat atggcgctgc cgcttgccaa cggtcataag atgctcgagc
    24961 cgaaagttgt ggcgcggctg gcgcagggct tgaagctgac gaaaaacgat acggttttgg
    25021 aaatcggcac gggatcgggc tatgcggccg cgctgttggc gaaactggct ggtcgtgtgg
    25081 tttcggacga catcgatgcc gaacagcaaa agcgcgccaa agcggttttg gacggcttgt
    25141 ctttggaaaa cattgattat gtgcaaaaca acgggctgac cgaactttcc gcaggcgcgc
    25201 cgtttgatgc ggtttatgtc ggcggcgcgg taaaccttgt gcctgaagtg ttgaaagaac
    25261 agttgaaaga tggcggacgc atggcggtta tcgtaggccg caagcctgtt cagcgcgcgc
    25321 ttttgattac gcgcaagggc gatgtgtttg aagagaaggt gctgttcgat accttggtgg
    25381 cgcatttgga tgataaggat gccgatccgt ttggcggttt cgatttttaa gatgttgaca
    25441 ggtcatgccg tctgaaagcg ggtttggggc ttcagacggc attttgcttg gtttttttgg
    25501 gggggtgcga tggatattgt gcaacttggg cccgcggaat tgaaagcgtg gatggacgaa
    25561 gggcggatgt tttgtttgtt ggacgtgcgt acggatgaag aaacggcggt ttgttcgctg
    25621 ccaaatgcgc tgcatatccc gatgaatctg attccgctgc ggcagaacga gctgccggat
    25681 gatgtgccgc ttgtggtgta ttgccatcac ggtatccgca gtctgcatac ggcgatgtat
    25741 ttggcggacg cgggttttga aaacctgtac aaccttcagg gcggcatcga tgcatgggcg
    25801 gccgagattg acggcaacat gatgcgttat tgaggcaggc atttcagacg gcattcctta
    25861 aatgcgtgta cctgtggtgt tccaaaaggt cgtctgaaag tgcagcttct gcgaagctaa
    25921 agcgcagtat caacgaagtt aaaatttgcc tgaaccttaa aggcagcctg caccccaatc
    25981 ccctctcccc gtgggagagg gctaggacga ggctggcaaa ctgaaggttt gcttgggcgg
    26041 cattttcaat gtgcaggct
//
LOCUS       DAWGJV010000008        25972 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWGJV010000008 DAWGJV010000000
VERSION     DAWGJV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.008"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            660..2108
                     /gene="gnd"
                     /locus_tag="WHQ94_000249"
     CDS             660..2108
                     /gene="gnd"
                     /locus_tag="WHQ94_000249"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ2127601.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIVIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2171..3442
                     /gene="waaA"
                     /locus_tag="WHQ94_000250"
     CDS             2171..3442
                     /gene="waaA"
                     /locus_tag="WHQ94_000250"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ2127602.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TVKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGASARIAEAVREAVCGHRGR"
     gene            3483..3932
                     /locus_tag="WHQ94_000251"
     CDS             3483..3932
                     /locus_tag="WHQ94_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ2127603.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3965..4939
                     /locus_tag="WHQ94_000252"
     CDS             3965..4939
                     /locus_tag="WHQ94_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ2127604.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            5008..5083
                     /locus_tag="WHQ94_000253"
     tRNA            5008..5083
                     /locus_tag="WHQ94_000253"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5041..5043,aa:Lys,seq:ttt)
     gene            5303..6556
                     /gene="murA"
                     /locus_tag="WHQ94_000254"
     CDS             5303..6556
                     /gene="murA"
                     /locus_tag="WHQ94_000254"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ2127605.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRLKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6637..7815
                     /locus_tag="WHQ94_000255"
     CDS             6637..7815
                     /locus_tag="WHQ94_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ2127606.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELCKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAGGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7892..8140)
                     /locus_tag="WHQ94_000256"
     CDS             complement(7892..8140)
                     /locus_tag="WHQ94_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ2127607.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8231..9148)
                     /gene="ftsX"
                     /locus_tag="WHQ94_000257"
     CDS             complement(8231..9148)
                     /gene="ftsX"
                     /locus_tag="WHQ94_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ2127608.1"
                     /translation="MSIIHYLSLHVEFARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLTRDKRLDNIRFISKEDG
                     LEELQTNLDQNLVSMLDGNPLPDVFIVTPDPATSPDQMQAIYKDITKLPRVESATMDT
                     EWVQTLYRINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEELEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLSAWLLAEVRPLVDAIFKPYGLNIGWRFFTFSEVL
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(9145..9795)
                     /gene="ftsE"
                     /locus_tag="WHQ94_000258"
     CDS             complement(9145..9795)
                     /gene="ftsE"
                     /locus_tag="WHQ94_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ2127609.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERAHIAIEKVGLNGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9989..10471)
                     /locus_tag="WHQ94_000259"
     CDS             complement(9989..10471)
                     /locus_tag="WHQ94_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ2127610.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTDAVKLAHSKCR
                     "
     gene            10595..10948
                     /gene="arsC"
                     /locus_tag="WHQ94_000260"
     CDS             10595..10948
                     /gene="arsC"
                     /locus_tag="WHQ94_000260"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ2127611.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10945..11601
                     /locus_tag="WHQ94_000261"
     CDS             10945..11601
                     /locus_tag="WHQ94_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ2127612.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGWDFFNWLFH
                     "
     gene            11793..13187
                     /locus_tag="WHQ94_000262"
     CDS             11793..13187
                     /locus_tag="WHQ94_000262"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ2127613.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGSATYDRRWRPEVGKTLPEIPSDVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIDANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13208..13603
                     /locus_tag="WHQ94_000263"
     CDS             13208..13603
                     /locus_tag="WHQ94_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127614.1"
                     /translation="MPSDVGIRLQTAFKRKLNMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13607..14098
                     /locus_tag="WHQ94_000264"
     CDS             13607..14098
                     /locus_tag="WHQ94_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2127615.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14221..16798)
                     /gene="mutS"
                     /locus_tag="WHQ94_000265"
                     /pseudo
     CDS             complement(14221..16798)
                     /gene="mutS"
                     /locus_tag="WHQ94_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
     gene            17249..18253
                     /gene="gap"
                     /locus_tag="WHQ94_000266"
     CDS             17249..18253
                     /gene="gap"
                     /locus_tag="WHQ94_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ2127616.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18323..18442)
                     /locus_tag="WHQ94_000267"
                     /pseudo
     CDS             complement(18323..18442)
                     /locus_tag="WHQ94_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18511..19191)
                     /gene="tsaA"
                     /locus_tag="WHQ94_000268"
     CDS             complement(18511..19191)
                     /gene="tsaA"
                     /locus_tag="WHQ94_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ2127617.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19306..19941
                     /locus_tag="WHQ94_000269"
     CDS             19306..19941
                     /locus_tag="WHQ94_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ2127618.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGIDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            19978..20946
                     /gene="waaC"
                     /locus_tag="WHQ94_000270"
     CDS             19978..20946
                     /gene="waaC"
                     /locus_tag="WHQ94_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ2127619.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21173..21922
                     /locus_tag="WHQ94_000271"
     CDS             21173..21922
                     /locus_tag="WHQ94_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ2127620.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21933..22868
                     /locus_tag="WHQ94_000272"
     CDS             21933..22868
                     /locus_tag="WHQ94_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ2127621.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            22931..23542
                     /locus_tag="WHQ94_000273"
     CDS             22931..23542
                     /locus_tag="WHQ94_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ2127622.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYKVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(23594..23911)
                     /locus_tag="WHQ94_000274"
     CDS             complement(23594..23911)
                     /locus_tag="WHQ94_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ2127623.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24069..25340
                     /gene="purD"
                     /locus_tag="WHQ94_000275"
     CDS             24069..25340
                     /gene="purD"
                     /locus_tag="WHQ94_000275"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ2127624.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGKLDSVAAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25374..>25972
                     /locus_tag="WHQ94_000276"
     CDS             25374..>25972
                     /locus_tag="WHQ94_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ2127625.1"
                     /translation="MTDMLQTAFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6245 a         5974 c         7304 g         6449 t
ORIGIN      
        1 ttcatgggaa tgacgggatg taggttcgtg ggaatgacgc ggtacaggtt ttcgttacgt
       61 atccccatcc gaaagtttat ttatacacaa gcaatccaaa gttatctgac tgaaatattg
      121 tgagatttac ccgcaaatct cattttttcc accacttata cacaaattcg gatgcgcact
      181 ctgccgattt gcagaaataa gacattcgtg ccgacacctg accatttcca cacgttccaa
      241 acacgcttcc ggcttaaatc tccacagtat tcccgttctt aaaggcgcca gcagccattc
      301 tgtaaagaaa agtaggatgc catctgcaca acaagcaaac tatccacaaa acttattccc
      361 cataaaaaac aatccacagg tatgcacaaa agggaatccg ccttaccgac accctaaaaa
      421 aaagaaatcc tgatgatttt taaaataaaa aaaaagttac ccacagaaaa tttctcctat
      481 caatataatc atcgttttat tatttaaatt ttatatttta tttataaccc ttcagcaaaa
      541 cggtacagca aagcgtaaag gcatcttttc aaaaaattaa aaagttaagg attgttaaac
      601 aagtgtaaat cgggtagagt tgcggtcgat aaattaaatc tatgacggag cttcctacaa
      661 tgaaaggcga tattggcgta atcggtttgg cggttatggg gcaaaacctg attttgaata
      721 tgaacgattg cggatttaag gttgtcgcct acaaccgaac aatcggtaag gtggacgaat
      781 ttttaaacgg cgcggctaag ggaacggata ttgtcggcgc gtattccctg caagacttgg
      841 tcgataagct ggcaaaaccg agaaaaatca tgatgatggt tcgcgcaggt tctgtggttg
      901 acgactttat cgaacaactg cttccgttgt tggaagaagg cgacattgtt atcgacggcg
      961 gtaatgccaa ttaccccgac acgacacggc ggacacatta ccttgccgaa aaagggattt
     1021 tgtttgtcgg ggcgggcgta tccggcggcg aagaaggcgc gcggcacggc ccgtccatta
     1081 tgccgggcgg ggataagcgc gcttgggaag ccgtcaaacc gatttttcag gcgattgccg
     1141 ccaaaacgcc gcagggcgaa ccgtgttgcg actgggtcgg caaggacggc gcggggcatt
     1201 ttgtcaaaat ggtgcataac ggcatcgaat acggcgatat gcagttgatt tgcgaagcgt
     1261 accagtttat gaaagatggt ttgggattgt cctacgatga aatgcaccgc gtgtttgccg
     1321 agtggaacaa aaccgagctg gattcttatc tgattgaaat cacagcggca attttgggct
     1381 ataaggacga aggcggtgaa ccgctggtcg agaaaatcct cgatacggca gggcaaaaag
     1441 gtacgggcaa atggacgggc atcaatgcct tggatttggg tattccgttg acgctgattt
     1501 ccgaagcggt attcgcccgc tgcgtttcgt cgttcaaaga acagcgcgtg cagaccggca
     1561 agctgttcgc ccgaaccgtt acgcccgtcg aaggcggcaa acaagaatgg gttgaggcgt
     1621 tgagacaggc cctgcttgca tccaaaatca tttcctacgc acaaggcttt atgctgatcc
     1681 gcgaagcggg cgaaagctac ggctgggatt tggactacgg caacactgcg ctgctgtggc
     1741 gcgaggggtg catcattcgc agcgcatttt taagcaatat ccgcgatgcc tatgaaaaca
     1801 atcccgattt ggtgttcttg ggtgcggatc cgtatttcaa aaatattttg gaaaactgcc
     1861 tgccggcgtg gcgcaaggtg gttgccaagg cggtcgaatg cggcatccct atgccctgca
     1921 tggcttcggc gattacgttc ttggacggct acacgaccga acgtctgcct gccaacctct
     1981 tgcaggcaca gcgcgactac ttcggcgcgc acacttacga acgcaccgac aaaccgcgcg
     2041 gcgagttttt ccataccaac tggacgggca agggcggaga taccgcttcg acaacctacg
     2101 atatttgatt ccgttgccga aatgccgtcc gaaccgttcg gacggcattt gttttgcagg
     2161 aaatagaaag atgttccaat ggctttatga tgtattgtgg ctgcttgcgc cgatatggat
     2221 acggcgttat ttggacaaac gctccggaag tgccccggca tatcgggcgc atcgggacga
     2281 gcgtttcggc aagccgtatc cgaatcccgt taccggagcg gtttggatac acgccgtttc
     2341 ggtcggggaa acgcgtgccg cccaaccttt gatacgcgag ttgcggcagc gttttcccga
     2401 tgcgccgctg ctgatgacgc agatgacccc gacgggacgg gaaaccgcgc aagttctgtt
     2461 tcctgatgca caatgccgct atcttccgta tgacaaaaaa acgtgggtac ggcagttttt
     2521 gcgcgaacac cgcccgatgt tcggcatttt gatggaaacc gaaatctggc ccaatctgat
     2581 gaaggaatgc cggcgcacgg gtgtgccgct gtttttggcg aatgcgcggc tgtcggaaaa
     2641 atcgctaaac ggttatctga aagtccgcag cctgatccgt cctgccgccg cttcgctgac
     2701 gggctgtctg gcacagacgg aggcggatgc ggcgcggttg gcgaaattgg gcgcggcatc
     2761 cgtgcaggtg tgcggcaata ccaaatacga cctgatgccg tcggaacaga tgaaaacgct
     2821 ggcggggcag tttgaaaaac gcatcggaga ccggcaggtt gccgtgtgcg gcagcacgcg
     2881 cgtttatcgg ggtgaagacg aggcggaaaa actgctggcg gcgtggcaac aatatcgcgg
     2941 cgatgccctg ctggtcgtcg tgccgcgcca tcccgagcat tttcagacgg tatttgaaac
     3001 ggtaaaacgc ttcgggttta aggttcagcg gcgcagcgac ggtttgccgg tcgaacccga
     3061 tacgcaggtg tggataggcg acagtatggg cgagctgtat gcgtattacc tgtgcgccga
     3121 tgtcgctttt gtcggcggca gtctggtcga ttcgggctgt cagaacatca tcgaaccgct
     3181 ttcctgcggc gttccgacga tattcggctt ttcgacctac aatttttccg aagcctgccg
     3241 gcacgccttg gcatcgggtg cggcggttca agtcgaatcg gcggatgcgt ggcgtgaagc
     3301 cgttgaaaaa accttatcgt ccgagggggg cggaatgcag atgcaggcgc gcgtggacgg
     3361 ctttatcgca caacatcgcg gagcgagcgc gagaatcgcc gaggcggtgc gggaagcggt
     3421 atgcggacat cgggggcgat agtgatacaa tccgtatccg agttttccgg ttggagcatc
     3481 gtatgagtat ttatgccgtc gcgcacatcg tccacctgta ttgcgccatc gcctttgtcg
     3541 gcggcgtgtt ttttgaagtg ctggttttgt ccgtcctgca tacgggacgg gtgtcgtgcg
     3601 aggcgcggcg cgaagtggaa aaggcaatgt cttaccgcgc cgtcagggtg atgccgtttg
     3661 tggtcggact gctgttcgcc agcggcatcg tgatggcggc aaaccgctat ctttctatat
     3721 tgggcgaacc gtttgccact tccttcggta cgatgctgac gctgaaaatc ctgttggcgt
     3781 tcagcgtgtt ggcgcacttc gccatcgccg tcgtcaaaat ggcgcgttcc acactgaccg
     3841 tcggctggtc gaaatacata cacaccgtcg tctttaccca tatgctgctg attgtctttt
     3901 tggcaaaagc gatgttttat atcagctggt aacacaacta catttcaaat caggagattg
     3961 aaccatgaca gaatatcccg gcatcgggtc gccgttgttt tacggcgttt tttttgcggc
     4021 agtgctggtc atgattgcct tggatatgtt ttcgctgaag aaaaacagca gccataaagt
     4081 cggcgtcaaa gaagccttgg catggagcgg cttgtgggtt gccgtatcct gcctgttcgc
     4141 aggctggctg tatttcaaac tcgccggcaa tcccggctac ggcgcggcag tcgcaaaaga
     4201 aaaagtattg gaattcttta ccggctacat tttggaaaaa tcgttggcag tcgacaatat
     4261 tttcgtgttc ctgatgatat tcggctactt caaagtcgca ccgcagtttc agcaccgcgt
     4321 gctgctgtac ggcgtattgg gcgcattggt attgcgcact gtcatgattt tcgtcggcgc
     4381 ggcactggtt cagcagttcg agtggattct gtatctgttc ggcgcgttcc tgctctatac
     4441 cggtatacac atgatgaagc ccgaaggcga tgaggaaggg gatttggcaa acagcaggct
     4501 gctgaatgcc gtcaagaaag tcgttccggt cggcacggaa tttcacggcg agaaattttt
     4561 tactgtcgaa aacggtaaaa aaatcgccac gccgctgttt ttggtgctgg tcatgattga
     4621 attgagcgat gtcgtgtttg ccgtggacag catccccgcc gtctttgccg ttaccaccga
     4681 tccgtttatc gtgctgactt ccaatatttt cgctattttg ggcttgcggg cgatgtattt
     4741 cctgctggcg gatgtggcgg aacgctttat cttcctgaaa tacggcttgg cattcgtgtt
     4801 gggttttatc ggtgtgaaaa tgctggtaat gcattgggtg catatcccga tttccgtttc
     4861 gctgtcggtc gtgttcggcg cgttgggtgc atcgatactg acctcgttaa tttatacgaa
     4921 aaaacagtcc gataaataaa atgccgtatg agcgccgaca aaatggttga cagcatcagg
     4981 cttgatgtat agaatttcga gcttatcggg tcgttagctc agtcggtaga gcagcggact
     5041 tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataaaa caaaaaaagc
     5101 tgagttttca aaactcggct ttttttgtct tccgacaatc cttgttcgga aagcgcggca
     5161 tttcgtcaaa ttctttggtg cggcttccgt atttttcccg aaaatccctt ataattccgc
     5221 ctctgtaacc ttcatcgggt gatgctttat gcgttgtccc gagtattgaa atattacaag
     5281 tacccactag gaaagaacaa acgtggacaa actgaaaatc tccgcaaacg gcccgctcaa
     5341 cggggaaata acggtctcgg gcgcgaaaaa cgcggcattg ccgctgatgt gcgcgggttt
     5401 gctgacatcg ggtacgttgc gcctgaaaaa cgtccctatg ctggcagatg tgaaaaccac
     5461 acaaaagctg cttcagggga tgggcgcgcg cgtcctgacc gacaatatca gtgaatttga
     5521 aatcaacggt ggtacggtaa acaacacctg cgccccttac gaattggtcc ggacgatgcg
     5581 cgcctcgatt ttggtgcttg gcccgacgct ggcgcgtttc ggcgaggcgc aagtcagcct
     5641 gccgggcggc tgcgccatcg gttcgcgccc tgtcgaccag catttgaaag gcttagaagc
     5701 gatgggtgct gagattgtta tcgaacacgg ttacgtcaaa gccaagggta agcttaaagg
     5761 cacgcgtgtg gcaatggatg tcgttaccgt cggcggcacg gaaaacctgc tgatggcggc
     5821 gacgctggcg gaaggtacga cggttttgga aaactgcgcc atcgagcctg aagtggtcga
     5881 tttggcggaa tgcctggtca aaatgggcgc gaaaatcagc ggcatcggta cgtccacaat
     5941 gattgtggaa ggtgtggacg agctgcacgg ctgcgaacac agcgttgtcc ccgaccggat
     6001 tgaagcgggg acgttcctgt gcgcggtggc gataaccggt ggcagggtgg ttttgcggaa
     6061 tgccgcgccg aaaacgatgg aagtggtgtt ggacaaactg gttgaggcag gtgcggtgat
     6121 tgaggcgggc gacgattgga ttgccatcga tatgcgtcaa cgtctgaagg cggtggacat
     6181 ccgtacggtc gtccaccccg gcttccccac ggatatgcag gcgcagttta tggcattgaa
     6241 tgccgtggcg gagggaagct gccgcgtggt ggaaacgatt tttgaaaacc gctttatgca
     6301 cgttccggag ttaaaccgga tgggggcgaa cattacaacc gagggcaata cggcatttgt
     6361 gcagggtgtg gaacagcttt ccggcgcagt cgtcaaggcg acggatttgc gcgcgtccgc
     6421 cagcctcgtt atcgccggtt tggcggcgcg cggcgaaacc gtggtcgaac agatttacca
     6481 cttggatcgc ggttatgaaa atattgaaaa aaaactcggc agcgtcggcg cgaaaatcga
     6541 gcgcgtatcc ggctgatcgg gttgcgatgc cgtctgaagc cttcagacgg catcaattgt
     6601 ttctgtatcg caaacactga ttgaaaagga ctgattatgg catttttaaa actgaccgaa
     6661 caaaacgtgc agggcaaaac cgtcctcatc cgcgccgata tgaacgtacc gttcaaagac
     6721 ggcaaaatca gcgacgacac ccgtatccgc gcctcgctcg cgtccatcca atactgcatg
     6781 gacaacggcg cgtccgtcat cgtgatgacc cacttgggcc gcccgaccga aggcgagttc
     6841 caccccgaag acgacgttgc gcccgttgcc gcgcatttgg gcggcctgtt gggcaaaaac
     6901 gtgaaagtat tgaacgactg gcgcgaaaac aaaccggctt tgaacgcggg cgatgttgtg
     6961 atgctgcaaa acgtgcgtat caacaagggc gagaagaaaa acgatttaga actgtgcaaa
     7021 gcctacgctt ctttgtgcga cgtgttcgtc aatgacgcgt tcggcaccgc ccaccgcgcc
     7081 caagcctcga ccgaagccgt cgcccaagcc gcccccgttg cctgcgccgg cgtattgatg
     7141 gcgggtgaac tcgacgcttt gggcaaagcc ttgaaacagc ccgcgcgccc gatggttgcc
     7201 atcgttgccg gcagcaaagt gtccaccaag ctgaccatcc tcgaatcgct ggcggacaaa
     7261 gtcgaccaac tcatcgtcgg cggcggcatt gccaacacct tcctgttggc aggaggcaaa
     7321 gccatcggca aatctttggc ggaacacgat ttggtggaag aatccaaaaa aattatggcg
     7381 aaaatggcgg ctaaaggcgg ctccgtaccg ctgccgaccg atgtcgtcgt tgccaaagcc
     7441 tttgccgccg atgccgaagc ggtcgtgaaa gacattgccg acgttgccga agacgatatg
     7501 attttggaca tcggcccgaa atccgccgcc gcgcttgccg atttgcttaa agccgccgac
     7561 acggtcgttt ggaacgggcc ggtcggcgtg ttcgagtttg accaattcgc aggcggtacg
     7621 aaagcccttg ccgaagccat tgcccaaagc aaagcgttct cgattgcggg cggcggcgac
     7681 acgctggcgg cgattgccaa attcggcgtt accgaacaaa tcggctacat ctccactggc
     7741 ggcggcgcgt tccttgagtt cttggaaggc aaagagctgc ccgccgtagc cgctttggaa
     7801 aaacgcggcg cgtaaacggt tagagataaa aaaatgccgt ctgaaacgaa atggtttcag
     7861 acggcatttt tgtgtggcgt ttattgtgct tttattgtgc tttagctgcc cattccgccg
     7921 gagtggcggc aaccgaaatg gaaagtgcgt gcagttcgtt gctttccagc tgggctttga
     7981 gtccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat tctgatgaaa
     8041 cgatgacggc gaaaaaatgg tgtccgtcgc cttctacttc gatgtgttcg cattttgcca
     8101 cgccttcaat catggctttt acttgttccg aggtcagcat ggtaggttcc ttgttggaaa
     8161 aatgcgccat tatactggca aattgaaatg ccgtctgaaa acgggttcag acggcatttt
     8221 tgacggtgtt ttattttttg gctttgaagc cgagcaggtg ttgggtggtg gccagccatg
     8281 cgccgaatat gcccagcgcg atgacgaagc cgaataccag caacacttcg ctaaatgtga
     8341 agaaacgcca gccgatgttc aggccgtagg gtttgaaaat cgcgtccacc agcggccgta
     8401 cttcggccag cagccaagcc gacaaaccca agctgacggc ggcggagaaa atactctgcc
     8461 acattgcctg ataaaggaaa ggacggcgga tgaaggaagc gggcgcaccc aacagcttgg
     8521 tgatttccaa ctcttccttg cggctgagga tttgcaggcg gatggtgttg tgtgccacca
     8581 agacaaaggc catgcccaaa gtcagcgaga ggaaccacaa aatcttgcgg ataaattcgt
     8641 taatacgata aagggtctgt acccattcgg tatccatagt tgccgattcg acgcgcggca
     8701 gtttggtaat gtctttatag atggcctgca tctgatccgg agaagtggca gggtcgggcg
     8761 taacgataaa gacgtcgggc aacgggttgc catcgagcat ggaaacgagg ttttggtcga
     8821 gattggtctg taattcttcc aaaccgtctt ctttgctgat aaagcggatg ttgtcgagtc
     8881 gtttgtcgcg cgtcagcagg ctgcgcacgg tatcgctgtc gctttgtgcg gcggcggttt
     8941 ccatatagat tgtgatttgc ggcgactcgt tgagtttgcc taaaacgctt tgtccgcttt
     9001 ggatgcccaa atacataaac agcggcaggg tcatggcgac ggcgagcatt atgagggtaa
     9061 gcagtgtgcc gaaaggttgg cgcagaagct gtttgagcgc ggtgcgcgcg aattcgacgt
     9121 gcagcgagag gtagtggatg atgctcatgc gagtcgtcct ttcgagaggc gcaggatgcg
     9181 gtgtccgtag tccgccatca gggtttcgtc atgggcggca acgatgacgg tagttcctgc
     9241 ttcatggaag gttttgaaca attccatgat gtcgagcgcg taggcgcggt cgaggttggc
     9301 ggagggttcg tcggcaatca gcaggctggg ctggtgaacg acggcgcggg caatgcacag
     9361 gcgttgttgt tcaccgcccg agagggttac gggatcgtcc aattctcgcc cgttcaagcc
     9421 gactttttca atagcgatgt gggcgcgttc ttcggctttg cgcggcggat aaccgataat
     9481 ccgcagtggg aggatgacgt tttgcaggac gttgcggtcg tagaggattt tgtggtcttg
     9541 gaacacgatg ccgatatgtt ggcgcataaa gccgatttgg ttgtcggtca gtgtgccgag
     9601 gtcttggttg ttgaagtgga ctttgcccct gcttggtttg gtaatgcccg aaatcagctt
     9661 gaggacggtg gatttgcctg aaccggagtg tcctgcgata aaaatcatct cgcctttgtt
     9721 gatttggaag ctgacgtttt tcagtgcttc aaaaccgccg ggataggttt tggaaacttg
     9781 ttcgaaacgg atcataggag gtcctgtaag gaagggcggc atatcgtttc gggcggttgc
     9841 gtccgcaggg atatgccgtc tgaacaatag gtaagacagt gccgattata aagattccgc
     9901 ctgcttttag atagtggata agtttggcgg atatgaaggt cgggcgggaa aaatgccgtc
     9961 tgaagcggat tcagacggca tccggcgttt aacggcattt tgaatgggcg agtttgacgg
    10021 cgtcggtcag gcttttttcg ttcacttccc cggtaatggt ttgtttgtat ccgcatttcg
    10081 gggcttcgac gacggtaaag ggcagtacgc cgacattgtt tccgtaggat ttcataaagt
    10141 ttcggctgtt cgccccggtg taacgccaaa tcgggtagga aacaggagtt tgtttgagga
    10201 agttgccgat attgtcggat gtgtcgagcg cgatgccgac catatcgacg ctgccttttt
    10261 tctgcgcttt gtaccatttg gacatggcag gcatctcttt tcggcacgga ccgcaccagg
    10321 ttgcccaaag gttgacgatg cgtacggggg ctttgagtga ttgcaggctt tgcggggtgt
    10381 tgtctttcca tccggccagt tcgtctgccg aggcggtatg tgcggcaaat gtggcggcaa
    10441 gggtgattgc ggcaagattc aagtatttca tcgttttgtc ctttgtatgt cgattcgggt
    10501 attgtaaacc gtttggtacg gttgtaggcg ggatattgag taaaaccctg tgtaagccga
    10561 taataagggg attgtcgaga agtaaggaaa tgtcatgtct gaaatcaaaa ttttccacaa
    10621 tccgcgttgc agcaaatcgc gtgccgcctt gtccctattg gaagaacgcg gcattgctgc
    10681 cgaagtggtc aaatatttgg atacgccgcc cgacttgtcc gaattgaagg atattttcaa
    10741 caaattgggc ttggcatcgg cgcgcgggat gatgcgtgtg aaagatgatt tgtacaagga
    10801 attgggtttg gacaaccccg atttggacaa tgacgcgctg ctgcgtgcca tcgccgatca
    10861 tcccgccctg ttggagcgtc cgattgtttt ggcgaacggt aaggcggctg tcggcaggcc
    10921 gttggaaaat atcgaagcgg tattgtgaac aaacggctgt tttgcagccg caacggcttg
    10981 cgctattacc tgttgggcgg tttctgtttg agtgtttttc cgcttttgct ggtgtttgcc
    11041 tcatccgttt gggcggttta tcggacgggc gggcaggttt tgccgccgta tgtccgggcg
    11101 gatgccgcgc tggtgttggg cgccgccgcg tgggacaaac gcccttcccc ggtttttcgg
    11161 gaacgcatca accacgccat cgcgctttat cagagccgca gggtaggcaa aatcgtgttt
    11221 acgggagggc gaaccaaaaa aggctatatg accgaagccg aggtcgggcg gcggtacgcg
    11281 ctcaaacagg gggttccggc gcgcaacatc ctgtttgaaa acacttcgcg caacacttat
    11341 gaaaacctga acaacatccg tccggttttg cgtgccaacg gtattgccag tgtggtgatt
    11401 gtcagcgatc cctaccattt ggcgcgggcg gcggaaatgg cggaggattt gggtgtccgc
    11461 gtttatatgt ctgccacgcc gaccacgcgc tttgatgcgg gaaataagaa aaaaatcttt
    11521 atgctgcaag agggatatgc gttatccctc taccgtttgg aaaaatgggg gtgggatttt
    11581 ttcaattggc tgtttcattg aacctttttt cggacgcaat cccaaaggaa cccggtcggc
    11641 ggcgcgtgat acactgttcc gcgtgccgaa gcattgaaaa caaggaaaat accgtctgaa
    11701 acttatccgc aggcagggtt cggacggcat ttttaatgcc caagcacggt aaaaacagta
    11761 aaataagccg ttttaaagat tctggagcca tcatgaccgt caaaacccgt ttcgccccca
    11821 gccccaccgg ctacctgcac attggcggcg ttcgcaccgc cttgttttca tgggcgtttg
    11881 cccgccatca caaaggcgag ttcctattgc gtatcgaaga caccgacttg gcgcgttcta
    11941 ccgccgaatc cgtcaacatc atcctcgacg gcatgaaatg ggtcggtctc aattacgaca
    12001 acgccgacaa cgtcgtttac caaactcgcc gtttcgaccg ctacaaagag gtcattgccg
    12061 aacttttaga aaaaggccat gcctactact gctattgcag caaagaagag ctggaagcca
    12121 tgcgtgagaa agccgaaaaa gaaggctcgg cgacttacga ccgccgctgg cgtccggaag
    12181 taggtaaaac cctgcccgaa atcccgtccg acgtacaacc cgttgtccgt ttcaaaacgc
    12241 ctttggacgg tgtgaccaaa tggacagact tggtcaaagg cgaaatctcc atccccaacg
    12301 aagcactcga tgacctgatt atcgcccgcg ccgacggtac gccgacctac aacttctgcg
    12361 ttgtcgtaga cgactacgac atgggcgtta cccacgttat ccgcggcgac gaccatgtga
    12421 acaacacgcc gaaacaaatc aacatcttaa aagccatcga cgcgaacctg cccgaatacg
    12481 gccacctgcc gatgattctt aacgaacaag gcaaaaaaat ctccaaacgc agcggcgata
    12541 ccgttgccat taccgatttc ggcgcaatgg gcatcctgcc cgaagcgatg ctcaactatc
    12601 tggcacgctt gggctgggcg cacggcgacg atgaattctt cacaatggaa caattcatcg
    12661 aatggtttga tttgaaagac gtttccccgt ctccaagccg tatggacttg aaaaaactct
    12721 actggatcaa cggcgaacac atcaaaatca ccaccaacga aaaactggcc gaaatggtga
    12781 aaccgcgcct cgccctgcgc gatatccatg aaacaagcaa gcctgctttg gaagacgtgt
    12841 tggcattggt caaagaccgc gcccaagatt tgaacgcctt ggcagacgaa tgtctgtatt
    12901 tctacaaaaa acaagtccct gccgaagccg atgttgccaa acactgggac gacgaagccg
    12961 ccgctcgtat gctgcgcttt gccgaacgcc tcgaagggct ggaagactgg aatgccgaag
    13021 ccatccacga cctcttcaaa cccttctgcg acgaagaagg cattaaaatg ggcaaactcg
    13081 gcatgcccct gcgccttgcc gtctgcggta cggcgaaaac ccccagcgtc gatgccgtgt
    13141 tggcattaat cggcaaagaa gaagtgttga aacgcatccg cgcttaagtt atccacaggc
    13201 gtgggtgatg ccgtctgatg ttggaatacg gcttcagaca gcattcaaac ggaagttgaa
    13261 catgaaaaaa atattttatt ttctgatggt tgttttttct acaagcgtat gggccgggga
    13321 tgcggaagac aatctgctca gcatccaatc cggttatcgt gccttactgc aaaaacaaaa
    13381 caatctggac ggaaaaatca tcgggatgca gtcggatttg gaagatgcgc gcagacggtt
    13441 gcaggcggct caggcagaca tcgcccgttt ggaagcggaa attcctgcag caatggcgca
    13501 aaaagcccgg caggctgaag agctgaggca aatcggagtg cgtttggacc atgcttggaa
    13561 tgcggtttac ggcgcagggg gaacgaaagc gtcggggaat tgaccgatga attccctctt
    13621 tgtggacaat actgttttca ttacacggct gaaagccggg catatcggca ggttggttca
    13681 ggcgttgttt gaggagtggc acggatttga accgtggtct tctgtggata agattcatgc
    13741 ctattacggc aggtgtttga aggatgacga actgccgctg gcatttgcgg ctgtggatga
    13801 ttccggaatc ctgttgggtt cggctacggt caagcggcat gatatggaaa gttttccaca
    13861 gtatgaatat tggttgggtg atgtctttgt tttacctgaa tatcgcggaa aaggcattgg
    13921 caggaggctg gtcgcccact gcataggcgc agcgcgttcg ctggggataa agttcttgta
    13981 tctttatacg cctgatgtgc aaatatttta tgaatcattc ggctgggcgg ttgtcgggcg
    14041 acatttccat aacggtgaat gggttacggt tatgcgtttg gatatggata aggtttaagc
    14101 cgaatggaca aatgccgtct gagctgttcg gacggcattt ttatgtttgg aaacttcttg
    14161 tcagataatg tttatccaca gatttttcca tatggtttga tgctgttccg acaacggaaa
    14221 ttaagatacg gatttgcaca aatctttcag acggtacagt tctgacaatg cttcgcgcgg
    14281 ggtcaggctg tcgggatcga gtttttccaa ggctgttgcc agtatacctt caaaatgttt
    14341 ttcctctgct ttgtccacaa agttgcccac attcggttca tctccttttt cagacggcat
    14401 ggtactgaaa atatccagtt ggggacggtt cgcggcggct tggttttcca gttcgttcaa
    14461 atgcttttgg gcggatttca atgcgcgtac aggcaggccg gcgagtttgg cgacggcaat
    14521 gccgtagctt ttaccggcgg gacccggttg gatttggtgc aggaaaacga tgtcctgtcc
    14581 ctgttcgagc gcggaaaggt gcatattgac ggcggcggcg tgggcttcgg gcaggtaggt
    14641 cagctcgaaa tagtgggtgg caaacaggct gaaggatttg tttttttgca gcaggtgttc
    14701 ggcaacggcg tgcgcgaggg cgaggccgtc gaaagtggaa gtaccacgtc cgacttcgtc
    14761 cattaaaaca aggctttgtt cggtggcgtg atgcaggatg taggcggttt cgctcatttc
    14821 gaccatgaaa gtggagcggt tggaggcgag gtcgtccgat gcgccgatgc gggtgaagat
    14881 ttgatcgatg ggcccgattg tggcggcatc ggcaggcaca aaacagccgg tgtgtgccaa
    14941 taaaacaatc agcgcgactt ggcgcatgta ggtggatttg ccgcccatat tggggccggt
    15001 gagcagcatg aggcggtgtt tgtggtcaag gtcggtgtgg ttggcggtga agtggcgtac
    15061 ctgctgttcg acaacgggat ggcggccgtt ttcgatgtgg ataaccggat agtcggcaaa
    15121 ctcggggcgg acgaagttcc gctcttttgc caaggctgaa aatgtggaca atacgtccag
    15181 cgcggcggcg gctttggcgg ctttttgaag ctgcggcaat gccgtctgaa ggtttttcaa
    15241 tacgccgtca aagagttgtt tttctaaggc gagggcttgc tcttgagcag tcagcacttt
    15301 gtcttcaaag gctttcagtt ccggcgtttt taagggtttg ccggcgttgg tagtcggcag
    15361 gtgcttgttc ggcttgggtt ttggacaatt caatgtaaaa gccgtgaacg cggttgaact
    15421 cgactttaag tgtggacaaa ccggtacgtt cgcgttcctt ggcttccaaa tccagcaaaa
    15481 attcgtcgcc atggttttga atgcggcgca attcgtccag ttcgggatga aaaccgtggt
    15541 tgatgacatt gccgtctttc agccagacgg aaggttcggg caaaatggct tggcggagct
    15601 gttcggctgt ggataggttt tccgggaaaa cggctttgag ggtttccaac aggctgctgc
    15661 cgtttgcgga caagtcgatt tgtgccaact cgaacagact gtcgcgtaaa gatgccaaat
    15721 cgcgcgggcg ggcgttgccg acggcgatgc gggcagcaat gcgttcaatg tccgcaatgt
    15781 ttttcagacg gcattggagg ggtttgtatt ggctttccag cgcggcaacg gcttcttggc
    15841 gcgctcggat gtgggcgcgg ttgcgtaaag ggtggtgcag ccagagagcc aagaggcggc
    15901 tgcccatatg ggtagcgcaa aggtcgagcg tggacatcag ggtcggcgat tttttgccgg
    15961 agagggtttg cgtgatttcg agattgcggc gcgtggcggc atccataccg atatattggc
    16021 tgtcggtttc gagcgacagg ccgtccaaat gttgcggcat caggttttgc gtcagacgga
    16081 tatagttcaa cagtgcacct gccgcgccaa tcgcaacggc gtgttctttg ccgtccaaac
    16141 cgaagccgcg caaatcctgg cagccgaaat attccgtcag cagtttttcc cccgcgtcgg
    16201 cggcaaactg ccacgcgttc aggcgcgtaa cacccgatgc cgtctgaagt tgcggtgcgt
    16261 ttttactgtc aggcaacaga atttccgccg cctgcaggcg cgccagttcg tcgtccaatt
    16321 tatccacagt tgtcagcttg gttttgaatt cgccgctttg cagcgatgcc cacgccaaac
    16381 cgatgtattt tttgtcgggg gacacggcaa cgatgcggtt ggtttccttg tcttccagca
    16441 atgcggaatc ggtcagcgtg ccgggcgtta cgatgcgcac gactttgcgc tccacaggcc
    16501 ctttgcccgc gccgacttcg ccgacctgtt cgcaaatcgc cacgcttttg cccaacttga
    16561 ccaggcgcgc cagatattgt tcggcggcgt gaaacggcac gcctgccatt ttgaccggct
    16621 cgccatccac ctgtccgcgc gtggtcaggg taatatccaa aagttttgcc gcttctaccg
    16681 catcgtcgaa aaacatctcg taaaaatcgc ccatacggta aaacaccagt ttgtcggtat
    16741 gttgcgcttt gatgccgagg tattgctgca tcattgggga aacggcggat ttggacatgg
    16801 ggctgcctta tcattaggaa aacggtattg tagcaaaagg ttttgtcttc gacggaatga
    16861 gactgaccgt aagcctgtca ataaggttgt ggagacaact aggaaaacag caaagttatc
    16921 cacagaaata ttattaagtc aaaaaacatc cacgcttatc tgtcttatac agcaagtctt
    16981 aaactcaaac ttatttgaca aaaaggtaat gaaaatattg gatatttgct acttacgcac
    17041 aagcaggttg gcaatcatca aaatcatcat taaaatttta aattcaaata ttgatggtaa
    17101 aagttttgga aatctgtgca aacagtaaaa ttcatctata tcaaataagc ggcaagtagt
    17161 tagtagttag atgtaatagt ggaatgtaac tttatttcag aatacaatac aggtattgaa
    17221 atttcgtttc acagcttgga gtatatacat gagcattaaa gtagcgatta acggtttcgg
    17281 ccgcatcggc cgcctcgcat tgcgtcaaat tgaaaaagcc cacgacatcg aagtcgtcgc
    17341 cgtcaacgac ctgacccctg ccgaaatgct gctgcacctt ttcaaatacg acagcacaca
    17401 aggccgtttc caaggtactg ccgaattaaa agacgatgcc atcgtggtaa acggcaaaga
    17461 gatcaaagtt ttcgccaatc cgaatcccga agaattgcct tggggcgagt tgggtgtgga
    17521 tgtcgtcctc gaatgtaccg gcttctttac caataaaacc aaagccgaag cacacatcag
    17581 ggccggcgcg cgcaaagtcg tgatttccgc acccggcggc aatgatgtga aaaccgtcgt
    17641 atatggcgta aaccaagaca ttttggacgg cagcgaaacc gttatctccg ccgcttcctg
    17701 caccaccaac tgccttgccc cgatggcggc agtcctgcaa aaagagttcg gcgtagtcga
    17761 aggtctgatg accaccatcc acgcctacac cggcgaccaa aacacccttg acgcgccgca
    17821 ccgcaaaggc gatttgcgcc gcgcccgcgc cgccgcgctc aacatcgtgc ccaacagcac
    17881 cggtgccgcc aaggccatcg gtctggttat ccctgaattg aacggcaaac tcgacggctc
    17941 cgcccaacgc gtccctgttg ccaccggctc gctgaccgaa ttggtttccg tcctcgaacg
    18001 ccctgtaacc aaagaagaaa tcaacgccgc gatgaaagcc gccgccagcg aatcttacgg
    18061 ctacaacgaa gatcaaattg tttcttccga cgttgtcggc atcgaatacg gttcgctctt
    18121 cgatgcgacc caaacccgcg tgatgaccgt gggcggcaaa caattggtga aaactgttgc
    18181 ctggtacgac aacgaaatgt cctacacctg ccaactcgtc cgcactttgg aatactttgc
    18241 aggcaaaatc taaggcttga acaaacctgt ggataagtaa atgccgtctg aacacattgc
    18301 gttcagacgg cattgtttat ctgtatagtg gattaaattt aaaccagtac ggcgttacct
    18361 cgccttgccg tactatttgt actgtctgcg gcttcgttgc cttgtcctga tttaaattta
    18421 atccgctata acttgatatt gcggcttatg gcgaacaaac aacagccgtt tgttcaactg
    18481 ctatgcggca aaaccggatt tttgcccgat ttaaagcggg gttggggaaa gatcaataac
    18541 ggttgcacgg ttttcctcga tttgaaatct gacttcgtaa tctgcaatat tcatcacata
    18601 aatccgttcg ggaatattct gataggcggg gcgcggatct tgggcaatgc tttggctgat
    18661 aaggtttttg gtgtttgcag ataaattttc cgcgccgatg ttttcctgcc aaacgacttc
    18721 caactctacg ggtttgccgc tgacgaaacc agatgcggca tcgggtttgg attcgacaaa
    18781 ggggatataa ggcttgatgt ccacaatcgg cgtgccgtcc agcaggtctg cgccgctgca
    18841 atagaggcgg acgggtttgc cggtttcgat gcgttcgagt ttcaggagcg agagtccgag
    18901 atggttgggg cggtgggggc tgcgcgtggc gaatacgccc attttttgtt tgccgccgag
    18961 ccgtggcggg cgcaccattt gtgcccagcc ttcatccaat acgccgtgaa aaataaaact
    19021 tatccacaca tagtcgaagt gttccagtcc gcgtacgctg tctgcggtaa attcgggatt
    19081 cagctcgatg catacttctg cggcagagac caaaccgggc tggcgggcga tgccgaattt
    19141 ctgtttgtag ggcgatcggg cggtggcgat agggacgatg gtgtaagtca tgaggattgt
    19201 ggataagttt ttcggaaaaa ccttatctta tcacagcctg ttttaccgct tttgggcagt
    19261 aatttgttat aatacagggc attttaattg cgttgaaatg atgagatgat tgtttccatt
    19321 gatgttgatg cacaaaaaac ttttacgcct ttgtgtccta aagagctgcc tgtaaacgag
    19381 gggcatttga ttgtcgaaga attgaatgcc caagccgctt tggcggattt gcgcgtgatg
    19441 acgaaagatg cgcatcatgc ggcagcaaaa tggcttgtgg ataaccctgt tgatatgttg
    19501 aagccgacag gtttgcccga tgcggatttg acttgggtgg ctcatgcgat ggttggtacg
    19561 cgcggctatg aattgttgga cgggctgcct tgtgccaaag aatacgatta ttgcgtttgg
    19621 aaaggtattg atcctgaatt gcatccttac ggcgcgtgtt ttcacgatat tgaggaaaaa
    19681 ctaagcacag ggctgattga atggctgcgt tgtcaaaata cggatacggt tattatcggt
    19741 gggttagcta cggattattg tgttaaaaca acggttttgc agttactcaa aggcggtcgt
    19801 tggcaggttg tcgtcaatga agcggcttgt cggggtattg cgccggaaac catcgaaaca
    19861 gcatggcagg aaatgcattc tgccggggca atcatcttaa aaaacgctga tgaaataaat
    19921 aaatatatta ataatcaata agttaaaatt gtttcacgtg aaaccccctt cctaaatatg
    19981 aaaattttgc ttgtccgctt gtctagtatg ggcgatttaa ttcacacttt gcccgcaatc
    20041 gaagatttgg cgcgacaatg tcctgatgta gaactgcatt ggctatgtga ggctggattt
    20101 gcagatattg cgcgcctgca tccgtttgta aaaaaaatcc atgtgatgaa atggcggcaa
    20161 tggcgcaaac atctctttcg ggctgaaact tggcgggaaa tgggtcatct gaaacagact
    20221 ttgcggcagg aagtatttga tttcgtattg gacagtcaag gtctgattaa aagcgcgtgt
    20281 ttcgctaaaa tggcgaaatc ccctatttat ggtttggata aaaacagtgc gcgcgaggga
    20341 tgggctgctt tggcgtatgt aaaaacatac gctgtaccga agggaaaaaa tgccgtttgg
    20401 cgcaaccgtg aactgtttgc ccaagtattt gggtatgtaa tgccggaaac gcaggtattt
    20461 ggcttgactg ttcctgaagc aggccgtctg aaaaatttag cgcagccgta ttatgcggct
    20521 ttgcatgcga ccagtcggga cagtaagtta tggcctgtgg aaaactggcg gtcgttgctg
    20581 caaaagttga atgaagaaca gcaatgcaat atttacctgc cttggggaaa tgaagatgaa
    20641 aaaacgcgtg ccaaacaaat tgcagacgga ctgccgtttg ctattgtgtg cgacaaaata
    20701 aatttattgc aggcagcgta tctgctgaaa tacgcggtcg gaattgtcgg cgtggatacc
    20761 ggtttgctgc atttggcaaa tgccttggaa aaacctgtgg tcggcattta taccgatacc
    20821 gatccgatta aaacaggcgt tcaggtttcg ccaattgcaa aaaatttggg caatatcggg
    20881 cagattccga ccgcagattt ggtttatcaa gcgctgatgg attgtgttgc agcagacaaa
    20941 ggctaaaggg ttgtctgaaa ctgatatggc ggcatgatat tttccaattt attaaataca
    21001 ggaatgtgta tccgctagtc tgagtaaatg ctttaaaaat ggtataatcg gcaagcatta
    21061 tccgaaagat ttttgaatga attcgttttt aaggaaaacg gatttggttt tatagggaag
    21121 aaggtttggt ttcgctggat ggtgaaagca gcgttctttt taggagggtt caatgaaagc
    21181 actggtcgca gtaaagcgcg tagtggacta caacgtcaaa gttcgtgtaa aagccgatgg
    21241 ttcggatgtg gatatcggca atgtcaaaat gtcgatgaat ccgtttgacg aaatcgctgt
    21301 ggaagaagcc gtccgtttga aagaagccgg aaaagtaagc gagatcgtag cggtttcttt
    21361 gggcgaaaag aaatgcgaag aaacgttgcg taccgctttg gcgatgggtg ccgaccgtgc
    21421 aattcatgtt gaaaccgatg cgaaattgga gccgctggca gttgccaagt tgctgaaagc
    21481 cgttgcggac aaagaaaatc cgcagatttt ctttttgggc aaacaggcga ttgatgatga
    21541 tgccaaccaa gtggcgcaaa tgctggctgc tttgctgaat gcggcgcaag gtacgttcgc
    21601 gtccaaagta caaattgaag gcgacgaagt gcagattgtg cgggaaatcg atggcggcga
    21661 agaaaccatc gcattgaaac tgcctgctgt tatcagtgct gatttgcgtt tgaacgagcc
    21721 gcgctttgtc aaactcccca atattatggc ggcaaagaaa aaacctctgg aaaaactggc
    21781 gcctgccgat ttggctaccg atattttacc tcgtctgaaa acggtgaaat ttgccgaacc
    21841 taaggcgcgt caggcaggcg taaaagtagc aagcgttgcc gaattggttg aaaaattgaa
    21901 aaacgaagcc aaagtgattt gaggaggctg aaatgagcgt attgattatt gtcgaacacg
    21961 acaacaaaca gttgaatcct accactttgc atgctgttac cgctgccgcc aagctgggta
    22021 aagtcgattt attggttgcc ggaagcggtg catctgccgt agtagagtcc gcaaaacaag
    22081 tggcgggtgt ggaaaaagtt ttggttgcag atgctgccca ttatgccgaa ggtttggctg
    22141 aagagttggc tccgctggtt gttaaattgg cagcggatta ccgctatgtt gcagcaacgg
    22201 caaccacatt cggtaaaaac cttttgcccc gcgtagccgc cttattagac gtaccgcaaa
    22261 tttctgattt gaccgagatt gtagataaca cgacttttgt acgtccgatt tatgcaggta
    22321 atgcatttga aaccgtgcaa gccaatgcgg aaaaattggt gctgaccttc cgtgcgacgg
    22381 cttttgacgc agcggcggtg caaggtggta atgctgaggt aattaatatt gaaacaaccc
    22441 ctgcccaaaa cctgagtcgt tttgttaacc gtcagctttc ccattccgat cgtcccgaac
    22501 tgactcaggc aaaagtgatt gtttctggtg gtcgtgcgtt gggtagtgcg gaaaaattta
    22561 atgaagtgct gacaccgttg gcggatgttt taggtgcggc aatcggtgca tcccgtgcgg
    22621 cagttgatgc cgaatatgcg ccaaacgatg ctcaagtcgg acaaaccggt aaagtagttg
    22681 cgccgcaact ctattttgca atcggtattt caggtgcgat tcaacacgtt gccggtatgc
    22741 aagacagtaa ggtgattgtt gcaatcaata aagatgctga tgcgccgatt ttcaatgtag
    22801 ccgattacgg attggttggc gatttatttg aaatcgttcc acaattaaca gaagcattga
    22861 aaaattaatg tttcacgtga aactttcaga cgactttgtt attaatgagg ttgtctgaaa
    22921 acttttcaac atgattacga cttataaaac cattacttcc ccgacgcagg ctgggtttaa
    22981 agataaaggc agccgtttta tcgcatttgc ctatccgatt cggacattgg ctgatgtgaa
    23041 aaaatacctc gatccgttaa aggaagagca ccataaagca cgacactggt gttatgccta
    23101 tcgtttgggc gtggatggta tgcaatttcg tgccaacgat gatggagagc catcaggaag
    23161 tgctggacga ccaattttgg gacagattga ttcggtgggc atcaccgatg ttttggtcgt
    23221 ggtcgtccgc tatttcggtg gtactttatt gggcgtttca ggtctgatac atgcgtacaa
    23281 agaggcgacg gctcaggcgt tggcgattgc ggaagtgatt gaaaagaata ttgaaaaaac
    23341 cgtttggctg aaatgtgaat atccattttt gaatgaagcg atacgcatcg ccaaacaata
    23401 tcaggcggat atattggagc aggatttgca gttggattgc cgattgacgg taagtttgtc
    23461 gttggcaaat tataaagtct gtgttgcggc ttggaagaat acacggcaga ttgaagtaaa
    23521 tacggaaaag ccttttgaat gaaaggtcgt ctgaaatgct ttgtgatggt atttcagacg
    23581 acctcttcgc tgactaatct tcttgtgtat atttgtctaa ggcatcgacg ctggagcgca
    23641 aatataacca agccagttga tggaattcgc ccagcgcgtt ccatagcgca tcttcactca
    23701 taatgctgta ttcgccttct gtgcgcaaat ccacctccgc tccatcttca atagcccgat
    23761 gacaagcctg atattcgtgg gtataaaaat tgtccatatc gcgaatcaaa gcgacggcat
    23821 gtttccaacg gcggatgtct tcttccaatt gcggtagtaa atgacaccat tcgcggtatt
    23881 tgctttggat ttcatcaata cgtttttgca tggttgcttg tcctttttta atttgtgaat
    23941 aagttgaata ggttgcagag tggaatgaag cggtcttttg tttgattcta ctaaaatttc
    24001 ttcagatggg ataaaagtcc ctttgtatcg agttttcgtt aaaagtacat atttgtcaag
    24061 gagatggcat gaaactgctg gttatcggta atggcggtcg cgaacacgcg ctggcttgga
    24121 aattggcgca gtcgcctaaa gtggaaacag tatttgttgc gcctggtaat gccggtacag
    24181 cgattgaacc caagttgcaa aacatcgact tgactgcgca tcaggatttg attgaattct
    24241 gtcgtaaaga aaatattgtt tttactgtcg tcggccctga agcgcctttg gcggcaggtg
    24301 ttgtggatga tttccgtgcc gcaggactaa aaatattcgg tccgacacaa tatgcggcgc
    24361 agttggaaag ttccaaagat ttcgccaaag catttatggc gaaatacaat attccaaccg
    24421 cgcaatatca aacctttgaa aacgccgatg ccgcacatga ttacgtcaat cagaaaggtg
    24481 cgcctatcgt tatcaaagcc gatggtttgg cggcaggtaa aggcgtgatt gtggcgatga
    24541 ctttggatga agcgcatgct gcgattgatg atatgctgct ggacaacaaa atgggcaatg
    24601 ccggcgcgcg tgttgtgatt gaagatttcc tgcaaggcga agaagcgagt tttatcgtca
    24661 tggttgatgg caataatgta ctgcctatgg caaccagcca agaccacaag cgtcttttag
    24721 acggcgacaa aggtctgaat acgggcggta tgggtgcgta cagccctgcg cctgtggtaa
    24781 cgcctgttgt gtacgaacgc gcgatgaatg aaattatttt gccgaccgta gcagggatga
    24841 aagcggaggg gcatgagttc accggtttcc tgtacgcagg tttgatgatt gatcaaagcg
    24901 gtgcacccta tacgattgag tttaactgcc gtttcggcga tcctgaaacc cagccgatta
    24961 tgagtcgtct gaacagcgac ttggcggatt tggttgaagc ggcaatagac ggtaaacttg
    25021 atagtgttgc tgccgaatgg aatccacaaa ctgccgtagg tgtagtgttg gcggcgcaaa
    25081 actatcctga aacgcctaaa aaaggcgatg ttatttccgg tctggatgct gccaaccagg
    25141 taggcaaagt tttccatgca ggtacaacgg caaacgagaa aggtgacgta ttgaccaacg
    25201 gcggacgcgt attgtgtgtt gtgggattgg gcgacaatgt ggcgcaagcg aaagctaagg
    25261 catatggcgc gttggaaaaa atcagcttcg acgggatgca ataccgtaaa gacattgccg
    25321 ataaagcgat taaccgttaa ttagaaagag gatgatgccg tctgaagcca agtatgactg
    25381 atatgcttca gacggcattt tatattatgt tatttccgag aattatcgct gcatcagcgc
    25441 aacgtaagtt aagcgtttat ttaaaaaaag gcggtttggt cgcttatccg accgaatctt
    25501 gttatggctt agggtgtctt ccgacattag ccaaagcact cggtaagttg gcgcatttga
    25561 aaaaaagacc gcaacacaag ggtatgattg tcatcgggaa tcaatttgaa caattgcagc
    25621 ctttacttca aatgccgtct gaaaatcttc aggatatgct tagaaaagaa tggcccgccc
    25681 ctaaaacgtt cctattatcc gccaaatcct gtgtcttacc tgagttgcgc gggaaacagc
    25741 gcagcaagtt ggcggtcagg gttcccgccc atgtaggtgc gcgccgcttg tgtcaggctt
    25801 tgcaaacgcc tttggtttcg acttcgtgca accgcgcagg caaacgcgcg tgtcggacgg
    25861 agagggaagt cagaaggcaa ttcggacgcg atgtgtggat tgttggtggg agaatcggca
    25921 ggcagaaatc gccgagtcag attattgacg gggaaacagg taaacgtttg cg
//
LOCUS       DAWGJV010000009        24158 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWGJV010000009 DAWGJV010000000
VERSION     DAWGJV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.009"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..255
                     /locus_tag="WHQ94_000277"
     CDS             <1..255
                     /locus_tag="WHQ94_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2127626.1"
                     /translation="IKRHSKYLIGGNLRLWQKRFWEHTIRDEADFACHFDYLHFNPVK
                     HGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIEYD"
     gene            327..488
                     /locus_tag="WHQ94_000278"
     CDS             327..488
                     /locus_tag="WHQ94_000278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127627.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            538..822
                     /locus_tag="WHQ94_000279"
     CDS             538..822
                     /locus_tag="WHQ94_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127628.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(947..1663)
                     /gene="trmB"
                     /locus_tag="WHQ94_000280"
     CDS             complement(947..1663)
                     /gene="trmB"
                     /locus_tag="WHQ94_000280"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ2127629.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTSAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSQPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1785..3188)
                     /locus_tag="WHQ94_000281"
     CDS             complement(1785..3188)
                     /locus_tag="WHQ94_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ2127630.1"
                     /translation="MNNPDLLYHQALERLSQEQYDFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPHQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDNEKACRYLQEAINSGH
                     EQKSFLQELLQQWQNAV"
     gene            complement(3307..5193)
                     /gene="uvrC"
                     /locus_tag="WHQ94_000282"
     CDS             complement(3307..5193)
                     /gene="uvrC"
                     /locus_tag="WHQ94_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ2127631.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCSAPCVGHISEEDYHDSVREASTFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLMRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVVAASKEDLEQVEGISKALAETIY
                     EHLH"
     gene            complement(5257..7419)
                     /locus_tag="WHQ94_000283"
     CDS             complement(5257..7419)
                     /locus_tag="WHQ94_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ2127632.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMD
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDDLYRI
                     AAAVEQNATHPLARAIVSAAQTRSLEIPAAQNAQTVVGAGIAAEVEDVGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7558..8508
                     /gene="trxB"
                     /locus_tag="WHQ94_000284"
     CDS             7558..8508
                     /gene="trxB"
                     /locus_tag="WHQ94_000284"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ2127633.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8664..9032
                     /gene="rpsF"
                     /locus_tag="WHQ94_000285"
     CDS             8664..9032
                     /gene="rpsF"
                     /locus_tag="WHQ94_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ2127634.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAAAQ"
     gene            9033..9335
                     /gene="priB"
                     /locus_tag="WHQ94_000286"
     CDS             9033..9335
                     /gene="priB"
                     /locus_tag="WHQ94_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ2127635.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            9342..9572
                     /locus_tag="WHQ94_000287"
     CDS             9342..9572
                     /locus_tag="WHQ94_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ2127636.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9589..10041
                     /locus_tag="WHQ94_000288"
     CDS             9589..10041
                     /locus_tag="WHQ94_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ2127637.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADAQARQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VATGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(10234..11040)
                     /locus_tag="WHQ94_000289"
     CDS             complement(10234..11040)
                     /locus_tag="WHQ94_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2127638.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11041..11787)
                     /gene="pssA"
                     /locus_tag="WHQ94_000290"
     CDS             complement(11041..11787)
                     /gene="pssA"
                     /locus_tag="WHQ94_000290"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ2127639.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(11861..12265)
                     /locus_tag="WHQ94_000291"
     CDS             complement(11861..12265)
                     /locus_tag="WHQ94_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127640.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            12371..12853
                     /locus_tag="WHQ94_000292"
     CDS             12371..12853
                     /locus_tag="WHQ94_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ2127641.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(12905..14116)
                     /locus_tag="WHQ94_000293"
     CDS             complement(12905..14116)
                     /locus_tag="WHQ94_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ2127642.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSIGEWGLPSTMFGLFAAGFMYFVFAVLIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14339..16777
                     /locus_tag="WHQ94_000294"
     CDS             14339..16777
                     /locus_tag="WHQ94_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ2127643.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            16985..18295
                     /locus_tag="WHQ94_000295"
     CDS             16985..18295
                     /locus_tag="WHQ94_000295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ2127644.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAQ
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAMFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18391..19005
                     /gene="clpP"
                     /locus_tag="WHQ94_000296"
     CDS             18391..19005
                     /gene="clpP"
                     /locus_tag="WHQ94_000296"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ2127645.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDAMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(19062..20327)
                     /gene="ispG"
                     /locus_tag="WHQ94_000297"
                     /gene_synonym="gcpE"
     CDS             complement(19062..20327)
                     /gene="ispG"
                     /locus_tag="WHQ94_000297"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ2127646.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLAFSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20343..21104)
                     /gene="pilW"
                     /locus_tag="WHQ94_000298"
     CDS             complement(20343..21104)
                     /gene="pilW"
                     /locus_tag="WHQ94_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ2127647.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(21107..22201)
                     /gene="rlmN"
                     /locus_tag="WHQ94_000299"
     CDS             complement(21107..22201)
                     /gene="rlmN"
                     /locus_tag="WHQ94_000299"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ2127648.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22345..22770)
                     /gene="ndk"
                     /locus_tag="WHQ94_000300"
     CDS             complement(22345..22770)
                     /gene="ndk"
                     /locus_tag="WHQ94_000300"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ2127649.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(22829..23980)
                     /locus_tag="WHQ94_000301"
     CDS             complement(22829..23980)
                     /locus_tag="WHQ94_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ2127650.1"
                     /translation="MSSRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQTAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5700 a         5850 c         6659 g         5949 t
ORIGIN      
        1 atcaagcggc acagcaaata tttaatcggc ggcaatctca ggctttggca aaaacgcttt
       61 tgggaacata ctatccgcga tgaggccgat tttgcctgcc attttgatta tctgcatttc
      121 aatccggtca aacatggcta tgtaggacaa atttccgatt ggcggttttc tacgtttcac
      181 cgttatgtca aacagggtat ttatccgcat aattggggtg gcggcaatgc ggacttttct
      241 attgaatacg attaaagtaa tgtcggattc aagaatccga cctacggaaa actaaagatt
      301 ttatacgggc tacggcttgc tggcttatgc gttggcttaa tttgaaaaac agcacatcgg
      361 ttcataatac acaaggtatt agaacaaaag aagcagttga acagggattg cgtggtaata
      421 atagacctga aaaaggaata ggtaatagat ggaattatga tggatttata aaggaattag
      481 cgaagtaaca ggtattctaa atatttctga taaatataaa gaacaaggag taaaaaaatg
      541 aaagtaatca ctattacatt gtttactagc tgcatactat ctggatgcta tgggcttgaa
      601 tatggaatga gtggcggcat tcctcatgat gactgggcaa gaggtagcca tgttagtcaa
      661 gccaaagcag atgctgcgtg gaaagaatgt tctgacaaat atatgagaaa tggtggttca
      721 ggattagtag aacaatgtat gaatgataaa ggattaatac gtttatctga aatacgtcgt
      781 aggcaaaaga gagcagaaac acaaagagca gtaattaagt aacaataatg aaatactact
      841 gttcccgaac aaagcggcat ttacgcaggt gatgacggct atattttgaa aaatgccgtc
      901 tgtaagcaaa cgtgcttcag acggcatttt tttatccggc ggtctgttat ccgatccgtt
      961 tgaataccaa gtcccaaacg ccgtgtccga ggcgtttgcc gcgcgcttcg aatttggttt
     1021 cggggcggta gtccggggtg ggggcgtagt ctgccgccgt attttgcagg ctgtcgaagc
     1081 tgctgaggac ttcaagcatc tgctgcgcat attcttccca gtctgtcgcc aagtggatat
     1141 agccgccggt tttgagtttg ggcagcagtt tggcgatgaa gggggcttgt atcagacggc
     1201 gtttgtggtg gcgttttttg tgccacgggt cggggaagaa tatgtggatg ccgtcgagcg
     1261 agccgtcttg cagcatattt tcgacaactt ctacggcatc gtgccgcatc acgcggatgt
     1321 tttccaaatg gttttcttct atgagtttga gcagattgcc tacgccggga ccgtgtacgt
     1381 cgatggcaag aaagtcggtt tcgggcagcc tgcgggcgat ttctgcggtt gccgtcccca
     1441 taccgaagcc tatttcgagg attttgggtt ggctgcttcc gaaacgggca tcaagatcgg
     1501 ccggtgttgc ttggtagtcg atgccgaagc tgccccataa ggtatcgatg gcgcgttgct
     1561 gcgcggaggt catgtggctt tggcggagga cgaaactgcg gatgctgcgg gggtgttcgg
     1621 cttgccagtt gttttgtgtt tggttttcgg cggtgtctgt cataatgctt tcgtgcgctt
     1681 gtgcaatcag gatgccgtct gaatgtgaaa tgcctgttcg gacggcggga aaggaaaggg
     1741 gcgattatac ctgtttcgtt tcggcagggt aggtgtaacg ctgttcagac ggcattttgc
     1801 cactgttgca acagttcttg cagaaagctt ttttgttcgt gtccgctgtt tatggcttct
     1861 tgcaggtagc ggcaggcttt ttcgttgtcc ggcccgacac cgtgtccgct gtaatggatg
     1921 caggcgaggt tgtagaacgc catactgtct ttttttgcgg cggctttttc aaaccatttg
     1981 cgtgcttcat tgtagtcggc ggtcataccc tgtccgaagt aatacatgct gcccagattg
     2041 gtttgggctt tgctgtggcc gagttctgcg gcttctgtgt agagtttgag tgcggctgca
     2101 tattcgggcg cgcattgtaa tccgtaatgc agggcttggg ctttttgata aagccgttct
     2161 gccctttgcc gcctgatggc ggaatcttta atgccgccgt attggttagg atggtttaac
     2221 gcgctgtcgg atatgagttt ctgataggcg gaaaggctgc cggcttcggc tgcctgccga
     2281 taataatgcc gggctttttc cgtatcgggg gctatgccca aactgtagcg gcagatgtcg
     2341 cccagtatcc gcaaaccttc gggatggcgt tcggcggcgg ctgcttcggc atgatgcagg
     2401 gcaagtttgt ggtttctttc cagatgcttg ccttgtaggt agatgtcggc aagtgcggca
     2461 tgggcatcgg ggtcattttc ctgtgccgcc tgttggaacc ggtaaacggc ttcatcggga
     2521 gccgtaggta ggatgagttc ggcaagcggg gtgtaggcgg gaatgtatcc ttgttgtgcg
     2581 gcggcgcgca aatgattgat agcttgggtc gtgtcggttg gtgtaccttg tccgaaacgg
     2641 tattgaaggc cgagctgcca ttgtgccgcc gccaagcctt gtgctgcagc ctcgcggtag
     2701 agaaggtgtg ccgtttcaaa atccggtggc gtaccgtact ggcagtattg ggcaagcaga
     2761 tattgtccgt gggggttgtt gttttttgcg gtcgtttttg cccatttcag ggcttcttca
     2821 aaatgtcggg acgcatacag aaggtacatc aggcggactt gtgcttcggg cagtccttcc
     2881 tctgccaacg gtctgtaaag cggtatcagg gtttccgggc gcgcgccttc caattcttgg
     2941 atatatgcca gattgtaacg cgcgtaggga tgcccctgtt cggcggcgat gcggagcatt
     3001 tccatacctt gttcgcggtc ttggtgcggg ctgtccgcgt ccatcaggtg ttttgctaac
     3061 tcgaatgcgg cggcgggatg acctgccgag gccgcttctg tcagcaggcg gcggacttcg
     3121 gtaaagtcat attgttcttg agacaggcgt tctaaggctt gatgatagag taagtcggga
     3181 ttgttcatat cagatcgttt tccgttttgt caaatcatcg ttattcgtta tgaagttagg
     3241 tttatatcgg cacgcctgcc tactgtatca tattttttac agacggattt tgtctttgaa
     3301 agcatgctaa tgcagatgct cgtaaatggt ttccgccaat gccttgctga tgccttcgac
     3361 ttgttccaaa tcttctttgc tggcggcaac gacaccgcgc aggccgccga agcgggtgag
     3421 cagggcctgg cggcgtttgc tgcctacgcc ggggatgtcg cttaaggagg aggtaacgcg
     3481 tgctttgtcg cgttttttgc ggtggcctgt aatggcaaag cggtgtgatt cgtcgcgcac
     3541 ggtttgcaat agatgcaagg ccgggctgtt gggcggcagg cggaagactt cgccggtaaa
     3601 aggcagtatg agttcttcca taccggcttt gcgctcaggg cctttggcga tgccgaccaa
     3661 gggaatgtgc agaccgagtt cttcccatac ggatacggcg acgccgattt gtcctttgcc
     3721 gccgtcaatc aatacgacat ccggccattt gacggtctcg ccgttggctt cggcttcctg
     3781 cattttgccg taacggcgca tcaatacttc gcgcatggcg gcgtagtcgt cgccgggctt
     3841 ggcggtcgtg atgttgtagc ggcggtattg cgaaggctgg atgttttgct catcgtacac
     3901 gacgcaggac gcaatagtgg cctcgccttg tgtgtggctg atgtcgaaac attcgaggcg
     3961 gttgaggccg tctgaatcca taccgaggat tttggcgagt tcgtcgatac ggtgttgctg
     4021 gctgctttgt tgcaggcggc gttgggcaat tgccatttgt gcgttttgct ccgccatttt
     4081 caaccagact ttgcgttcgc ctatggtttt ggtaacgaac tgcatttgtt tgccgtgttc
     4141 gctctctagg gcttctttca atgcgtcggg catggggaag ttgctgatga tgatgtcggg
     4201 tttgcttttg cccagatagt gttgggcgac gaaggcttcg gcgtagtctt gtccgtttgg
     4261 ttcgggatcg tttttggtgt cagggaaaaa acttttatcg ccgacgtgcc gtccgccgcg
     4321 gatgctgacc cagtgtacgc agacgatgcc gtctgaaacc gccagcgcga gcaggtcgat
     4381 atcgttggga ttgttcggat ttttactgtc gataaactga ttactctgca tgatgccgag
     4441 tgcttggatt tggtcgcggt agcgggcggc ttcttcaaac tgcaaattcg cggcggcggt
     4501 ctgcattttg tgttgcaggg tgcgggtcag ttcgtcggtc ttgccgttga gaaaagtcga
     4561 ggcctcgcgc acgctgtcgt ggtagtcttc ttcgctgata tggccgacac agggtgcgct
     4621 gcagcgtttg atttgataca gcaggcaagg gcggtcgcgg tgttcgaaca cgctgtcttc
     4681 gcaggtgcgc agcatgaaga ctttttgcag gacttggatg ctgtcgcgca cggcgttgct
     4741 gttaggatat gggccgaaat attgattagg ctttttcagc gtgccgcggt aatacgccat
     4801 ttgcggatat tgatggccgc tgagcatcaa ataaggatag cttttgtcat cgcggaaaag
     4861 aatattgtat ttgggcgaca gggctttgat gaagttgttt tcgagaatca gggcttcgga
     4921 ttcggagcgg gtgatggtgg tttcgatgtg gtgaacctgt ttcaccatca atgcgatgcg
     4981 cggggaatgg tcgtttttct ggaaatagcc ggacacgcgc cgcttgaggt tgacggcttt
     5041 gccgacgtat aagacgttgc cgctttcgtt gaaaaaacgg tatacgcctg gcaggttggg
     5101 caggtttttg aggaaaagtg ggatgtcgaa gtgttccgga aaacgggttt ctttattcat
     5161 atcaattact tgaaaaacgg tttcagacgg catattacca tatcgctgga gacagcctta
     5221 tccgttcgga caaggctgca gggcggttac atgctgctaa tcgattttta cccgtttcag
     5281 gcgcaaggca ttgctcaaca cggaaaccga gcttgccgcc attgccgcgc cagcgatgac
     5341 gggatttaaa aagccaagcg cggcgagagg aatgcccaag atattgtaga agaaggcgaa
     5401 aaacaggttt tgcttgatgt ttttcaaagt ggcttgcgac accagcagag catcggcgag
     5461 ttggttgacc gaatgctgca tcagcgtggc ggatgcggta tgttcggcaa cgtccgctcc
     5521 gcctttcatg gcgaagctga cgttagcggc ggcaagcgcg ggcgcgtcgt tgatgccgtc
     5581 gccgaccatc gccacggttt tgccggcggc tttgagtttt tgcacttcgg cagctttatc
     5641 gcgcggactc atgttgccga aggcgtgtgc gatgcccagt tgtttggcga cgtattcgac
     5701 cgtgccttgg ttgtcgccgc tcataatata gacatcgata ttgtgttttt tcagacggcc
     5761 tatggcttcg gcggtatcgg ctttcaacgc gtcggcaagt gcgaatgcgc cgatgggttt
     5821 gttatcgacc gagactgcaa caatgcttgc aatgtcccaa acgccgtctg aaaactcggg
     5881 caaggtaagt tcggcaaatt cggctttgcc tgctttcacc aaacccacgt cttccacttc
     5941 ggcggcaatg cctgcgccga caacggtttg agcgttttgt gcggcaggga tttccaagct
     6001 gcgcgtttgg gcggcggaaa cgatggcacg ggcaagcgga tgagtggcgt tttgttcgac
     6061 ggcagcggcg atgcggtaca aatcgtcttc gtcaaagccg ctgtcgggaa cacaataaac
     6121 ggcggcaacc tgcggcctgc cttcggtcag cgtgccagtt ttgtccaaca caacggcatc
     6181 gacgtgggcg gcttcttcca ttgccgctgc gtctttaaac caaatgccgt gtttcactgc
     6241 tttatccatg ccgaccataa tcgcggcggg tgtagccaga ccgagcgcac acgggcaggc
     6301 aatcaccaaa acggcgacgg cgtgcatcag cgcaaccgtc caatcgccct taatcagcca
     6361 agtaacaata aaagtcaaca acgcaatgcc cacgacggca ggcacgaata ccgcagccgc
     6421 tttatcggct acgcgcgcaa tcggtgcttt actgccttgt gcttcagaga gcgcgttcat
     6481 catgtcgccg agctgggttt ggctgccgag ctgcgtggcg cggtacacca cactgccttc
     6541 ggtcattaac gcgcccgcca acactttgcc gcccgccttt ttttcttcag gattggattc
     6601 gccggtaaga tggctctcgt ccgcccaacc gctgccgctt tcaatgatgc cgtctgcggc
     6661 aatgcgttcg ccgtggttgg cgcggataag gtcgccgatt tgcacttggt cgatgggaag
     6721 ctgtttccat tcgccgttgc gttgcacgtt gacttgggtt ggtgtaagtt tcaaaagcag
     6781 gcccaagctg ttcaggctgg attttttggt acggtgttcc aaaaatttgc ccagcgacac
     6841 aaaaccgatc accatcacgc ctgcttcaaa atacacatgc gccatgccgt gtgccgcatg
     6901 cggactgaaa aataacatat agacggaata cagataaatc gataccgtgc cgatggtaac
     6961 gagcacgtcc atattcgcca atccaccttt aatactcgcc cacgcgcttt tgtaaaacgg
     7021 gattgccagc caaagctgca ccacactcgc caatgcgaac tgccacaacg gcggaatcat
     7081 ccaatcgtgt ctgccgatca tcatccccgc catgccgata aggaacggga cgttgatggt
     7141 gaacagcagc cacagccgcc agccgatatg gtgttctgct tcgggttgcg gcaatgtatc
     7201 ttccgttttt tccttcgcgc cgtaaccggt tttctcaatg attttggcaa tgtcggctac
     7261 tgaggttttg ctgtcgtcaa acactacctg cgcctcctcg ctggcgaagt tcactcccgc
     7321 cgattcgaca aaatcttttt tgttcaacac tttttcaata cgcgaagcac atgcctgaca
     7381 ggtcatgcct tcgatttgga aacggatttt ttgttgcata agcgtgattc ctgctttgat
     7441 attcagacgg cagtcaaggt tgcgggatgc cgtctgaacg cccgtatggt cgaaatttct
     7501 caaacgcgcc gataattcga gtagaatccg aaaacttgac acagcaaagg aaaaacaatg
     7561 tcccaacacc gcaaactgat tattttgggt tccggccccg ccggatacac cgccgctgtc
     7621 tatgccgcgc gcgccaattt aaaccccgtc attattacag gtatcgcgca aggcgggcaa
     7681 ctgatgacca ccaccgaagt ggacaactgg cctgccgatg ccgacggcgt gcaagggccg
     7741 gaattgatgg cgcggtttct cgcccacgcc gaacgtttcg gaacggaaat catttttgac
     7801 caaatcaacg ccgtcgatct gcaaaaacgc ccgtttacac tcaaaggcga tatgggcgag
     7861 tacacttgcg atgccctgat tgtcgcaaca ggcgcgtccg ccaaatacct tggtttgccg
     7921 agtgaggaag cgtttgcagg gaaaggcgtt tccgcttgtg ccacctgcga cggtttcttc
     7981 tacaaaaacc aagatgttgc cgtagttggc ggcggcaata cggcagttga agaggcactc
     8041 taccttgcca atatcgccaa aaccgttacc ctgatccacc gccgcagcga gttccgtgcc
     8101 gaaaaaatca tgatcgacaa actgatgaaa cgcgtggaag agggcaaaat catcctcaag
     8161 ctggaaagca acctgcaaga agtactgggc gacgatcggg gcgtaaacgg cgcattatta
     8221 aaaaacaacg acggttctga gcaacaaatt gccgtcagcg gcatttttat cgccatcggg
     8281 cacaagccga ataccgatat tttcaaaggt cagttggaaa tggacgaagc tggttacctg
     8341 aaaaccaaag gcggcacggc ggacaatgtc ggcgcaacca atatcgaagg cgtatgggcg
     8401 gcgggcgacg taaaagacca tacctaccgt caggcgatta ccagcgcggc ttccggctgt
     8461 caggcggctt tggacgcgga acgctggctg ggcagccaaa acatttgaat gccgtctgaa
     8521 accgaaattt tcggcggaat gcaaattttt cgcaccaaat ccttgattcc gtcggcaaac
     8581 cccaatataa tgcctgctct ttgaaccttg ccttctgctt tcgcatggca gaaggctgtt
     8641 tttaccatcc ataaggagat aacatgcgtc attacgagat cgtgtttatc gttcatcctg
     8701 atcaaagcga gcaagtgcct gctatggttg aacgttacaa aaccatgatt gccgaagcaa
     8761 acggtaagat tcaccgtttg gaagattggg gtcgccgcca actggcttac ccgattaaca
     8821 aaatccataa agcccattac gttttgatga acatcgaaac cactcccgaa gtggttgaag
     8881 agttggaaac cgcattccgt ttcaatgatg cggtattgcg ccatctaacc atcaaaacca
     8941 aacacgccgt taccgaagca tcccctatgt tgggtggtga aaaggctaag aacctgctga
     9001 gcggtgcgtc tgaagaagcg gctgcccaat aattgggatt caataatctt gtttcgcttg
     9061 ccgcgttgat tgaaaaggtt ttccctattc gatatacgcc tgccggaatc tctgtcttag
     9121 atattatttt aaagcatgaa tcatggcagg aggaaaacgg gcagcaatgc cttgtccaat
     9181 tggaaattcc ggcacggatt ttaggcaggc aggcggaaga gtggcagtat cggcaaggtg
     9241 tatatgttca cgtcgaaggt tttttagctc aaaaaagcag acgttccctt atgccgatgc
     9301 tcaggataca aaatattcaa gaatataaag gttaaacgac aatggctcgt caatcattca
     9361 aacgtagaaa attctgccgt ttcacggctg aaaaaatcca agaagtcgat tacaaacaag
     9421 ttgatttgct gaaagacttt atctctgaaa acggtaaaat cattcctgca cgcatcacag
     9481 gaacgaaggc attctaccaa cgccaattgg ctgttgccgt aaaacgcgca cgcttcctgg
     9541 ctctgctgcc ttacaccgac caacacaaat aattttggag attgaatcat gcaaattatt
     9601 ctgttagaaa aaatcggcgg tttgggcaac ttgggcgaca tcgtaaccgt taaaaacggc
     9661 tatgcccgca acttcttgat tcctgctggt aaggcaaaac gtgctactga agcaaacatg
     9721 aaagagtttg aagcacgccg tgccgaattg gaagccaaac aggccgaaat tttggcagat
     9781 gcccaagccc gtcaggaaaa attggatggc caaaccatta ccgttgcaca aaaagccggt
     9841 gtggacggtc gtctgtttgg ttccgttacc aatgctgata ttgcggctgc aatcgttgcg
     9901 actggcatcg aagccgtgaa agcaaatgta cgtctgccga acggtcctct gaaagccgtt
     9961 ggcgagtacg aagtggaagt ggctttgcac actgatgccg ttgctaaaat taccgttgcc
    10021 gtcgttgccg caaccgagta attgaaatta tcagatgccg tctgaaaccc tgccgattgt
    10081 cggaatgtgg tttcagacgg tatttgtttt gttttccgta tattctgcaa aggtctcggt
    10141 caggttgtct gtccttttgt cggaacagga gactgggcag ctttgtcctg attaattact
    10201 tgtgcaaacg gtaaaaaggt tttttcgtgt gttttaaagc aggttgtaca gcatttttcc
    10261 ggcaatcaaa agcaacataa tgccgaagga ttttttgagt ttggcagaag aaagtttgtg
    10321 ggcggttttg acaccgagcg gggcaaaggc aatggttgcc gcgctgagga cggcgacggc
    10381 gggcaggtaa aggaagccca gtgacccttc gggcaatcct gcaatattca ggccgttgag
    10441 cagatacgat attgcgccgg agagtgcaat cggccaggca aggccggatg atgtgccgat
    10501 ggctttatgg gcggggaagc cgcagtggat taagaagggg acggaaagtg aaccgccgcc
    10561 tatgccgacc cagctcgaca ttgtgccgaa cagtgtggaa accgcagtca gtccgggcag
    10621 tccgggcagc gggcgggatg ccgtctgagg gtcggtatgc agtgttttga atgcgacggc
    10681 ggttaaaaac aggatgaaga aaatttgaag cccgaacgct gggatatatt ttgcggagag
    10741 tgcgccagcg aatacgccga ataccatacc cggcatcatc gtaaatacgg ttttccagtc
    10801 gaccgcctgt tttttgtgct gccccagcat actggaaaag gcggtgaaga ccatgacggc
    10861 gaaggatgtg ccgacggcga ggtgttgcgc gtaaggatgt tgtgccaaac cctgcaaatc
    10921 aagcacccat aaaacgacag ggacaatcag cgtgccgccg cctacgccga acaggccggc
    10981 aataaaacct gccgcactgc ctacggcaag caggattaag ataatgtccc aatgccacat
    11041 ttaatccgcc tttctgtatt ttttccagaa tcggcgtgcc gccataatgt agccggacag
    11101 gctgtatccg agaaagaaca ggaagaggac gagcgacggt tcccaagtga ccagaagcag
    11161 cagtaagacg gcaagcagca ttccgacaaa ggggacttgt ctgcggatgt tgatttcttt
    11221 aaaactccaa aaagggattt ggacaatcat cgacaggccg gcaaacagtg tgatgcccaa
    11281 tgcccaccag tggacggcgg ggaatttttc gacgctgtgg ttgacccaaa tcagcccgac
    11341 aatcagcgcg gcggcagtcg gactgggcac gccgataaac cagcgtttgt ccaccttgcc
    11401 gatgagcgtg ttgaacagcg caaggcgcag ggcggcgcag gcgcagtaga tgaaggcgac
    11461 ggaataaccg attttgccga actgccaaag ctgccatttg taggcaatca gagcgggagc
    11521 aacgccgaag ctgaccatat cggcaaggct gtcgagctgc tccccgaacg cgctttggct
    11581 gttggtcagc cgcgccacgc gcccgtccat accgtccagc aacatagaga tgaataccgc
    11641 gatggcggcg gtttcataac gtccgtgcat ggattgggtg attgcgtaaa atgcggaaaa
    11701 cagcgcggcg atggtaaagg agttgggcag caggtagata ctgttttgac gcagggagcg
    11761 ttgcggggtg ttggtcgggg tttccatagc tgaagtgtct cacggacgtg gagaatcgat
    11821 aggggattat aacccaaacc attatcgaac aatacgccct ttaggatgaa gccctcggag
    11881 gcggtttgtt tttctgatgg cggatgcttt gcggggcggt ttgactctgc ctgccaccgg
    11941 gtgtgttgtt tcggattttt gccggatgga tattgttgcc gggcatttct gggcttgccg
    12001 gaaggtatcg gggggagggc atcggaatat gcggcgcacc gttcaaaccg gggcggcgtt
    12061 tccctatttt cggggagttt gcaggcttca acaaattgaa agatattttt tgcatttccg
    12121 accgtatgct gattttgaaa cgggataaat tataggacaa ggtttttcgc ccgccgcaaa
    12181 ggttggccgc aatgccgtct gaaaacaacg gcttgccgct aaggcggcgg tatgcggatt
    12241 tgcgctgctg aaatcggttt gccatacccg gcggtgcaat ctgcggggag ggataagccg
    12301 ataaggcggg acttatcctg ttttttgttt ataatgcaac agaatttgga cgaaccgtcg
    12361 ggaatttatc atgatgaaaa agatactggc cgtatcggca ctatgcctga tgactgcggc
    12421 ggcacgggct gccgatactt acggctatct cgccgtttgg cagaatccgc agaatgcgga
    12481 cgatgttttg caggttaaaa ccacaaaaga agattcgacg aaaagcgaag cgttttccga
    12541 gttggaagcc ttctgcaaag gtcaggacac gcttgcgggc attgctgaag acgagccgac
    12601 cggatgccgg tcggtcgtgt cgctgaacaa tacctgtgtc gcgctggcat acccgaaagc
    12661 cttgggcgcg ctgcgtgtcg acaacgccgt cgtgattact tctccgcgtt ttacgagcgt
    12721 tcatcaggtc gcactcaacc agtgcatcaa aaaatacggc gtacagggac aatgcggctt
    12781 ggaaacagtg tattgcacat cttcttctta ttacggcgga actgtgcgct ctttgattca
    12841 aaatctcaaa taaaacggaa aatgccgtct gaaagatgtt cagacggcat ttctatatcg
    12901 acggtcagga ttctttcgga tcgggcagca ggctgttcaa cataatggca agtacggcgc
    12961 acaagcccac gccggcaaag ctgaagctgc ccaatttgag cgtcatgccg ccgatgcccg
    13021 tggtcagtac cgagctgacg atgaccaggt ttttcggcag catcaaatcg actttggcat
    13081 caatcagcgt tttcacgccc aaagaagcaa tcgtgccgaa cagcagcagc ataatgccgc
    13141 ccattactgg catcggaatg gaagccaaaa acgcattgaa tttgccgaaa aacgccatgc
    13201 agacggcaaa aaccgccgcc caagtcatga tgacggggtt gctgtttttg gtaatcatca
    13261 ccgcacccgt tacttcgccg taggtcgtaa ccggcgggcc gccgatcaga cccgcaacgc
    13321 acacgcccaa accgtcgcct gcaagggttt tgtccaagcc cgggtctttc gtatagtctt
    13381 tccccgtcac attgccgatt gccatgatgc cgccgatgtg ttcgatggcg ggggcgacgg
    13441 caacgggcag cataaacagt gccgcctgcc agttgacctg aggcgtttca aaatggggaa
    13501 cggcgaacca gggcgcgtgt gcaatgcttg ccgtgtccac cagtcccatc agcagtgcca
    13561 aaacataacc cgaagcgaca ccgatcaaga taggaatcag cttcatcatc ctgctgccga
    13621 aaaccgatac gatggcggta acggcaaagg taaagccgga aagaatcagt gaatcggtat
    13681 agtcgatgac ctgtttgccg tccgcctgac ccattgccat gctgcttgcc gccacggcaa
    13741 cagacagacc gatgaccatg atgacggggc cgatgacgac cggcggcagc agtttgtgta
    13801 ccgccgccag tccgcgccaa cgaattagca cggcaaacac aaaatacata aagccggcgg
    13861 caaacagtcc gaacatggtg gaaggcagcc cccattctcc gatggagtag ataatcggtg
    13921 cgataaaggc aaacgaagaa ccgagaaaaa tcggcacttt acgtttggtt gtaatttgga
    13981 acagcagcgt tcccaagcct gcgcccaaaa gcgcaagagc cggattcaga ccggtcagca
    14041 ggggaaccag caccattgcg ccgaatgcca caaataaaat ctgtgcaccg gaaacggcaa
    14101 gtttcagttg gttcatacca tcttctttca atcggttcaa acggcacgga ttatactggt
    14161 ttgccgaggg tctgcgtaaa aatacatacg tcgggcaaca gatatgccgt ctgaaaggca
    14221 atgtctgtct gttgcaggat gaaagcagga gaaatggagt ataatccgac actttccata
    14281 ttcaatccgc aagccgtccg gcttccttat ttcagacggc attttccggt aatacggcat
    14341 gacagaaaaa tcccataaaa aaacagcaaa aggcagggcg ggaagcccgt ccccaacgtc
    14401 cgcacgcaac aagaaagccg acaacggcgc acggggcaat aaagtttccg agcggctcaa
    14461 ggcggtcaaa gagttgcaga aaaccgaaac caaaaaggcg cgtcccgaac atgtcgtcaa
    14521 ccttatcggc gacgcactgt ggctgatggg tttggcggca accctgtatt tggcgatttc
    14581 cctgatcagt ttcgatatgg gcgatccgtc ttggtcgcac agttcgccgg ttgtggaaga
    14641 tgtcgccaat tggggcggac tgttcggcgc gtatgttgcc gatgtcggct attatctttt
    14701 cggctggtcg ttctggtggt ggatagcggc tgcctgcgtc gtgctgtata aaaatttccg
    14761 cctgcacgca aaacagacgg aaaacgaggc atacaaccac aaaatcgctg ccgccgcgct
    14821 gtttgtcctg acggtcttca gccccgtctt ggagtatttt gtgctgggcg gaaaatatgc
    14881 cgactccctg cctgtcggag caggcggtat ggtcggcata cgcgtcggcg cagtgtttgc
    14941 gtggctgctg gggaaatcgg gcagcctgct gattatcttg gttgttctgc tgttgtcgtt
    15001 gtccctactg gtgcagattt catggctgga atttttgaac ggtgcaggca gggcggttca
    15061 aaaccgcctg agtgccttat ccggcaaggt catggcttta ggaaaacgcc ggccgaatac
    15121 caaaacagac ggtgtcgata cccaaaatac acggcgcatg gtaaaagaag ccaagaatat
    15181 tacggccaaa cccgttgcct tgcccgaagg cagcagcagc aaccgcaaat ccgtcgcggt
    15241 ttccgtcgcg ccgccgccca aaattcaggt ttctctgttt gaagatgacg aacctcggca
    15301 ggcgggcgaa taccacaagc ctacattgaa cctattgcgg attcccgaca gcgagcccgt
    15361 cagcatcaat cccgccgaat tggagcgcac tgccgaacta atcgaatcca agctggcaga
    15421 attcggcatc ggcgtacaag tcgtatccgc cacatccggc cccgtcatca cgcgctacga
    15481 aatcgaaccc gcgcaaggtg tcaaaggcag ccaaattgtt gccttgtcga aagatttggc
    15541 acgctctatg tcgctgcaat ccgtgcgtat cgtcgaaacc atcgccggga aaaacacgat
    15601 gggcatcgag ttgcccaacg acaaacgcca agacgtgatg ttgagcgaaa tcttgtcctc
    15661 gcccgtgttt gccgaagcca aatccaagct gaccgtcgcg ctgggcaaag acattgccgg
    15721 tacccccgtt gtcggcgact tggcgaaaat gccgcacctt ttggtcgccg gtatgactgg
    15781 ttcgggcaag tccgtcggcg tgaacggcat gattatgtct atgcttttca aagctacgcc
    15841 cgacgaagtc cgcttcatta tgatagaccc gaaaatgctc gagttgagca tttacgacgg
    15901 cattccgcac ctgctctgtc ccgtcgtgac cgatatgcgc gaagcagggc aggcgttgaa
    15961 ctggtgcgtc gccgaaatgg aaaaacgcta ccgcctgctt tcccacgccg gtgtacgcaa
    16021 tctggaaggc ttcaaccaaa aagtcgaagc cgcaaaagcg gcaggcaagc ctttgcttaa
    16081 tccgttcagc ctgaaccccg acgagcccga gccgctggaa aaactgccgt tgattgtggt
    16141 cgttatcgac gaacttgccg acctgatgat gaccgaacgc aaagccgtcg aacagcaaat
    16201 cgcccgtctc gcccaaaaag cgcgcgccgc cggtatccat atgattgtcg cgacccaacg
    16261 tcccagtgtc gatgtcgtta ccggcctgat taaagccaac atcccgacgc gtatggcgtt
    16321 taccgtgcaa agcaaaatcg acagccgtac catcctcgac caaatgggcg cggacgaact
    16381 gctcaaatat ggcgattcgc tgttccttca gcccggcagt gccgaaccga cccgcctgca
    16441 aggcgcgttt gtttcagacg acgaagtaca tcaagtcgtc aactacgtca aatcgcaagc
    16501 cccagccgac tatattgaag gtctgctcag cggcgaggcc gcgctggaaa ctgccaatat
    16561 cgttaatccg aatgcagaca gcgacgaatt gttcgatcag gcagtcgcct atgttttgga
    16621 aagcaaaaaa acctccattt cgtctttgca gcggcagttg cgtatcggct ataaccgcgc
    16681 ggcaaacctg atggaggcac tggaaaatgc gggtgtcgtt tctcccaccg acctcaacgg
    16741 cagccgtaaa attttggcgc acaaggacca tttgtagccc gtattgcaaa atgccgtctg
    16801 aacggcggaa ttggcgtttc agacggcata ttatgtttca ggcgaaacat tgtgatatac
    16861 ttgccagcta aaatttcccc tttgcggcaa tgcggttcaa atatcgtacc gttgcgctgt
    16921 ttgcttcccc atgtagggaa gaaagtttat cattttatca acacaacaaa tttaagggct
    16981 tatgatgagc gtaactgttg aaactttaga aaatctggaa cgcaaagtag tgttgtccct
    17041 gccttggtcc gaaatcaacg cagaaaccga taaaaaactg aaacaaactc aacgccgtgc
    17101 aaaaatcgac ggtttccgtc cgggtaaagc acctttaaaa atgattgccc aaatgtacgg
    17161 tgcgagcgca caaaacgacg tgatcaacga gctggtgcaa cgccgcttct acgatgttgc
    17221 cgttgcccaa gagttgaaag tggcaggctt cccccgtttt gaaggcgttg aagaacaaga
    17281 cgataaagag tctttcaaag ttgccgccat ttttgaagtg ttccccgaag tcgttatcgg
    17341 cgatttgtct gcacaagaag ttgaaaaagt aaccgcttcc gtcggtgatg ccgaagtgga
    17401 ccaaaccgta gaaatcctgc gcaaacaacg tacccgcttc aaccatgtcg aacgcgaagc
    17461 tcaaaacggc gaccgcgtca tcattgactt tgaaggcaaa atcgacggcg aaccttttgc
    17521 aggcggcgca tccaaaaact acgccttcgt attgggcgca agtcaaatgc tgcctgaatt
    17581 tgaagccggc gtagtcggca tgaaggctgg cgaaagtaaa gacgttaccg tcaatttccc
    17641 tgaagactac cacggtaaag acgttgccgg taaaactgcc atgttcacca ttacgctgaa
    17701 caacgtttcc gaagcgactc tgcctgaagt cgatgcagat tttgcaaaag ccttgggtat
    17761 tgcggatggc gacgttgcca aaatgcgcga agaagtgaag aaaaacgtaa gccgcgaagt
    17821 ggaacgccgc gtaaacgaac aaaccaaaga atccgtaatg aacgcgctgc tcaaagccgt
    17881 agagctgaaa gcacctgttg ctttggtaaa tgaagaagcc gcacgcttgg caaacgaaat
    17941 gaaacaaaat tttgttaacc aaggtatggc tgatgctgcc aacttggatc tgcctttgga
    18001 tatgtttaaa gaacaagccg aacgccgcgt gtctttgggt ctgattttgg ccaaactggt
    18061 tgatgaaaac aaactggaac cgactgagga gcaaatcaaa gccgttgtcg ccaacttcgc
    18121 agaaagctac gaagatcctc aagaagtaat cgactggtac tacgcagagc cttcccgcct
    18181 gcaagccccg acttctttgg cgatagaaag caacgtcgtt gatttcgttt tgggtaaagc
    18241 caaagtaaac gaaaaagctt tgtcttttga cgaagtgatg ggcgcgcaag cctgattatc
    18301 ctgaaaatgc cgtcttaggg cagtttttga aagcaccgaa gccgctttta aagcccgctt
    18361 tggtgctttt acatcatgaa aggagacgaa atgtcttttg ataactatct tgtccctacc
    18421 gttatcgagc agagcggtcg cggtgagcgt gcattcgata tctattctcg gcttttgaaa
    18481 gagcgcatcg tattcttggt cggcccggta accgacgagt ccgccaatct ggtggttgcc
    18541 caactgttgt ttttggaaag tgagaatccg gataaggata ttttcttcta tattaactcg
    18601 ccaggcggtt cggtaacggc cggtatgtcg atttacgacg ccatgaattt tatcaagccc
    18661 gatgtatcga ctttgtgctt ggggcaggca gcaagtatgg gcgcgttctt attgtcggca
    18721 ggcgaaaaag gcaaacgttt cgccctgccc aacagccgga ttatgattca ccagccttta
    18781 atcagcggcg gcttgggcgg tcaggcatcc gatattgaaa ttcacgcacg cgaactgttg
    18841 aaaatcaaag aaaaactcaa ccgcctgatg gcgaaacatt gcggccgcga tttggcagat
    18901 ttggagcgcg acaccgaccg tgataatttc atgtccgccg aagaggctaa agaatatggt
    18961 ctgattgatc aaatcttgga gaaccgtgct tctttacagt tttaataaga aatctgagag
    19021 aaacaaatgc cgtctgaaaa cagttcagga cggcatttgt cttataggga ctgtataggg
    19081 atgattttgt tctggttgcg tttggagccg ttttcaccat agttggtttt gacatattcc
    19141 tctacaatag ctaggaattc agttgcaata ttatcacctt tcagtgtgac tttgcgctcc
    19201 ccgtccacat aaaccggcgc gacgggtgtt tcccccgtac cgggcaggct gatgccgatg
    19261 tcggccaatt tgctttctcc gggcccattg acaacgcagc ccattacggc aacgttcagg
    19321 gattcaaccc cgggataaag ggtacgccat atagacattt tttggcgcag gtaattttga
    19381 acatcttgtg ccagctcttg aaatacggta ctggtggtac gcccgcagcc ggggcaggcg
    19441 gtaaccatcg gcgtaaacga acgcaatccc atagtctgta aaatctcttg cccgacgacg
    19501 acctcctgag tacgcgggct gccaggttcc ggagtcagtg aaatgcggat ggtgtcgccg
    19561 attccttctt gaagcaagac ggataatgcc gccgttgatg cgacaatgcc tttgctgccc
    19621 ataccggctt cggtcaaacc caaatgcagc ggataggcgc aacggctgcc cagttcgcgg
    19681 taaacctgaa tcaaatcctg aaccgcgctg actttgcacg acaggatgat tttgtcttcg
    19741 ggcagtccca atagaacggc tttttcggca gattccaaag cggagacaat cagtgcttcc
    19801 ttcgtcactt cttcgggcgg tttcggcgcg gaaaaagcga ggttggcatc catcatacgt
    19861 ttggcgaggc tttgatccaa agaaccccag tttacgccga tgcggacggc tttatcgttt
    19921 tcagcagcag tccgaatcat aaaggcaaat ttttcatcgc cttttacgcc tttgccgaca
    19981 ttgccgggat tgatgcggta tttggacaat gctttgccgc attcgggaaa ttccgccaac
    20041 aggcgttcgc cgttgaagtg gaaatcgccg ataagcggtg tggcatagcc catatcgtcc
    20101 aagcggcggc ggatttcggc aactttggac gcggcttcgg ggctgttgac ggtaatacgc
    20161 accatttcgg atccggcatc gctcaattcc ttaatctgca atgcggtggc ttttgcatcg
    20221 gcagtgtcgg tgttggtcat agattggata acgacgggtg cttctgaacc gacggtaata
    20281 tgatcgatgc ggacttgatg cgtcttgcgg cgttggagtg tgttcatatg gtttgaatct
    20341 gtttattgac cggtgaggac ggtttgcaat tcttccgagt aggggaaatt cgcctgcaat
    20401 tgtgcttcat attcgtatgc cgcctgtgcg ttgccgaggg ctttggcaat tttccagcct
    20461 agcagcaaat catcggcctg aaggacttct accctgcttt ggtatttttt aaagtagtaa
    20521 tcggcatcgc ccaactgccc ggccagcatt ttggtgcgcg ccagttcttt aaatgcgggt
    20581 gggaactgcg gctgggcggc gagggaacgt ttcaaatagg cttccgccaa tccgaattgc
    20641 ccctgttttg cgctgcatat gcctttattc aggttggcaa tataaggggt cgggtaggtg
    20701 gggtcggcca gggctttgtc gaaatatgcc atagattcgg cagggcggtt gagcctgccg
    20761 cacaggaacc agccgtagtt gttgttgatt tcggcactgt cgggtttgat ggagagggct
    20821 tgccggaaac tttcctgcgc cttgtcgtta actttcaggt attgatagat ttcggcacgg
    20881 accagccagg caagctcgtt tttagggtct gatttcaagg cgtcttcaat acttgccgtc
    20941 gcctgacggt agtcctgacc gcgcatatat tccattgcca actgggtttt gatattggaa
    21001 acctgattgg ctttttctgc ccgcgagggg cggtaggaag tgctgcacgc gcccaaggca
    21061 aggacaagta ataaagagat tcgtttggat ggcttaaaag gcataattac ccctgttgtc
    21121 cgattaaaat ctgctgccat ttttgttggc ggcgcgtttt atcctgaacc tgccccgcca
    21181 actgtccgca ggcggcatcg atgtcgtcgc cgcgcgtttt tcgtacggta acgacaaatc
    21241 ctgcctgctg caaaatatcg cggaacacac ggatgttctc attgctggag cgttcgtatc
    21301 cggagtttgg gaagggattg aacggaatca gattgaactt gcagggaaca tctgtgacca
    21361 gttcgatcag ttcgcgcgca tgttgcgcct tatcgtttat tccgtccaac atgacgtatt
    21421 cgaaagtgat gaaatccctg ggtgctttga ccagatagcg ttggcatgcg gccatcaatt
    21481 ctttcaaggg atattttttg ttcaacggta cgatttggtt gcggacttcg tcattggaag
    21541 cgtggaggga aaccgccaaa gccaccggca tgacatcgcg caacctgtcc atttggggaa
    21601 ccatacccga agtggaaacg gttacgcggc ggcggctcaa accgtagccg tggtcgtcca
    21661 gcatgatgct taaggcggta acgacattgt cgaagttcgc catcggctcg cccatgccca
    21721 tcatgacgac gttggaaata acgcgctcgt ttttcggtgt aacgcccatc gctttgtttg
    21781 cccaccacaa ttgcccgatg atttcggcag cagtcaaatt gcggttgaag ccctgccgac
    21841 cggtcgaaca aaatgtacat tccaaagcgc agccgacttg tgaggaaatg cagagcgtgc
    21901 cgcgatccga ttcggggatg aagacggttt ccaccccgtt gcccgtaccg acatccaaaa
    21961 gccattttcg agtgccgtct gaagattttt gagacatcat cagcttggga atttcgatgc
    22021 ctgcctgttc gttcagttta tggcgcaacg attttgccaa atcggtcatt tcgtcaaaat
    22081 tttgcgcgcc ggattggtgc atccaacgca taacctgttt ggcacggaaa ggtttttcgc
    22141 ccatatcggc aaaatgtcgg gtcagccctt ggaggtcgta gttgagcaga ttggttttca
    22201 tgtatttgtg tttttcttaa attaaggctt caaataaaaa attgcagggc aggcaagttt
    22261 gatttgaagc cttttgcaac agagttttca gacggcatgg atatatttta tgccgtctga
    22321 agaggagttg tgcggtgtat tgtatcaacg ggggcagatt tcggtttggc tgaaaaagta
    22381 ggcaatttcc aaagcggcat tttccggagt gtccgagccg tgtacggcat tgatgctgac
    22441 cgaagtggca aagtccgcgc gtatcgtgcc ttcggcggct tcggaaggat tagttgcacc
    22501 catcagttcg cggtttttca gaacggcgtt ttcaccctct aatacctgaa tcataaccgg
    22561 accgccggtc ataaattcaa ccaatccggc gtagaagggg cggtctttat gaaccgcata
    22621 aaattcttgc gcctctttga gggtaagctg cttcattttg gcggcaacga ttttcagacc
    22681 gttctcctca aagcggctgt atattttgcc gataacgttt ttgccgacgg catcgggttt
    22741 gatgatggag atggtacgtt caatcgccat gctatatcct tatttttact taagaagaat
    22801 caaatcggta ttctatcaaa aaaataactt atccgcctga agccttggga gatagggtca
    22861 ggtgcggctg ttccaaataa acttcggact gcatttcgac catccggctg gcggtgcggg
    22921 taaattcttc ggcaaaatcg ccttctgtat agatatggtt gacatctacc gcgccggtgg
    22981 cacacagttt gacacggaaa tcgtagagta cgtcaatcag ccaagtcagc cgccgtgcct
    23041 ccgccttttc ttgcggtgag agttgttcca aacctgaaat aaaaaccatt tcataatgtt
    23101 cggccaaata caggtagtcg gactgtgagc gggggccgaa gcacagtgcg cggaaatcaa
    23161 accatatggc acggccggac tcggctttgt ggggaatctc ccgaccgtgg atggtgctga
    23221 tgccggggtt caaatcggta atgcctgtca tttctttgaa cagttttgcc agttttgcct
    23281 cattttcttc attggcaggc gtaaagaaaa tctcggcggg gcggagggta cgcagtcggt
    23341 agtcttcacc gccgtcaacg tttaagacgg tcaggctgga ctcgatgagc gcgattgtgg
    23401 gaagaaaact gctccggttt tgaccttgcg ggtagagttc ggaaggcgcg tagtttgaag
    23461 tcgccaccaa aacaacgccc tcgttaagca ggttttccag cagacggcct aaaatcattg
    23521 catccgcaat atcgctgaca tgaaattcgt caaaacataa tacgcgggtt tctttggcaa
    23581 tctcggcggc aacggatttc aacgggttgc tttcgctttt cagggttttc agcctctggt
    23641 ggatttctgc cataaaggca tgaaagtgga cgcggcgttt gcggcggtaa gggaggcagc
    23701 cgaaaaaagc gtccatcaga aagcttttgc cgcgtccgac cccgccatag aaataaagcc
    23761 ctttggggac ttgcggggag cgcaaactcc tgcctaaaaa acggtttctt ttgcgtttga
    23821 acatcatcaa ttcggtccaa agccgatcga ggtgttcgat ggcggctgtc tgcgcgtcgt
    23881 cgcggatgaa gttgggcagt tgtgaggcag cctgatacca ggtcagcggg ctgtggtttt
    23941 caaacggcgg ggctttaaaa agttggtctc tactactcat atttaacctt tttatacttc
    24001 ttgcggctaa gaaagaaaat gcttactgtc ggtattataa atgatttcct tcaaacttgg
    24061 cattttccgt ggaaaagaat aatgacgcgc acaagggggt atgagcaaag gcggcgtttc
    24121 aatatggtgc agatatgagc tcaaatgcct tttatagt
//
LOCUS       DAWGJV010000010        23772 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWGJV010000010 DAWGJV010000000
VERSION     DAWGJV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.010"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(93..932)
                     /locus_tag="WHQ94_000302"
     CDS             complement(93..932)
                     /locus_tag="WHQ94_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ2127651.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="WHQ94_000303"
     tRNA            1217..1292
                     /locus_tag="WHQ94_000303"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="WHQ94_000304"
     CDS             complement(1357..2550)
                     /locus_tag="WHQ94_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ2127652.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WHQ94_000305"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WHQ94_000305"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ2127653.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3806..4906
                     /gene="aroC"
                     /locus_tag="WHQ94_000306"
     CDS             3806..4906
                     /gene="aroC"
                     /locus_tag="WHQ94_000306"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ2127654.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4998..5423
                     /locus_tag="WHQ94_000307"
     CDS             4998..5423
                     /locus_tag="WHQ94_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ2127655.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5497..7482)
                     /gene="parE"
                     /locus_tag="WHQ94_000308"
     CDS             complement(5497..7482)
                     /gene="parE"
                     /locus_tag="WHQ94_000308"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ2127656.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7549..8073)
                     /locus_tag="WHQ94_000309"
     CDS             complement(7549..8073)
                     /locus_tag="WHQ94_000309"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ2127657.1"
                     /translation="MLDREGYRPNVGIILINNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWASVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            8239..9534
                     /gene="serS"
                     /locus_tag="WHQ94_000310"
     CDS             8239..9534
                     /gene="serS"
                     /locus_tag="WHQ94_000310"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ2127658.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYKFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9612..10610)
                     /locus_tag="WHQ94_000311"
     CDS             complement(9612..10610)
                     /locus_tag="WHQ94_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ2127659.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="WHQ94_000312"
     CDS             complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="WHQ94_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ2127660.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(11901..12671)
                     /locus_tag="WHQ94_000313"
     CDS             complement(11901..12671)
                     /locus_tag="WHQ94_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ2127661.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12674..13666)
                     /locus_tag="WHQ94_000314"
     CDS             complement(12674..13666)
                     /locus_tag="WHQ94_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ2127662.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TASGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQFPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13948..14631)
                     /locus_tag="WHQ94_000315"
     CDS             complement(13948..14631)
                     /locus_tag="WHQ94_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2127663.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILRFKPIARIMTPKRYEQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFIIMDGLAALISVPIWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14821..16155)
                     /gene="glmM"
                     /locus_tag="WHQ94_000316"
     CDS             complement(14821..16155)
                     /gene="glmM"
                     /locus_tag="WHQ94_000316"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ2127664.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRINGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIAAAIGGI"
     gene            complement(16285..17136)
                     /gene="folP"
                     /locus_tag="WHQ94_000317"
     CDS             complement(16285..17136)
                     /gene="folP"
                     /locus_tag="WHQ94_000317"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ2127665.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPCADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVIMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMVGELTGETDAAARVHGSVAAAVAAVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17276..18169)
                     /locus_tag="WHQ94_000318"
     CDS             complement(17276..18169)
                     /locus_tag="WHQ94_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ2127666.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18260..20374
                     /locus_tag="WHQ94_000319"
     CDS             18260..20374
                     /locus_tag="WHQ94_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ2127667.1"
                     /translation="MDLLSVFHKYRLKYVVAVLTILLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDTPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20438..21916)
                     /gene="ubiD"
                     /locus_tag="WHQ94_000320"
     CDS             complement(20438..21916)
                     /gene="ubiD"
                     /locus_tag="WHQ94_000320"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ2127668.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21936..22451)
                     /locus_tag="WHQ94_000321"
     CDS             complement(21936..22451)
                     /locus_tag="WHQ94_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ2127669.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYESDTFGMHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22458..22958)
                     /locus_tag="WHQ94_000322"
     CDS             complement(22458..22958)
                     /locus_tag="WHQ94_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127670.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            complement(23190..23759)
                     /locus_tag="WHQ94_000323"
                     /pseudo
     CDS             complement(23190..23759)
                     /locus_tag="WHQ94_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5384 a         6753 c         6217 g         5418 t
ORIGIN      
        1 tcctgatttt tgttaattca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttaaaatt ttgcaccgga ttggtttgcc
      121 atataatcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga cgggctggat acgggtctgc
      781 gttgccgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacagtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcgatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccaacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgtacg gtagcgatgc gtccttgcgc
     2281 gagcgcaggg ttgtctttgc cgaacaataa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgtggcg gcgcgggcgg tttcggcacg
     2401 gcgcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcagggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gcccggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcgcgtt cgaccgcatg ggtttccacc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttctgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga tggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcag catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtggacagaa attcgacggc ataccagcgt
     3361 tttttgagtt cgggggattg cgcggcggcg gcgatcagct cgggcatgag gcggttgaca
     3421 gcctcggaag ctgcttcaaa acggtcgcag cgtatcatgc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggta gtctttttta ccttgaagct gctgcgtgta gtcattcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattt gcctcgccgc acgcaaactc
     3721 tttgactgcc gccccttcaa tgacggacgg gcttttgtgc taaaatccgc catctttcca
     3781 cactataccg ataaagggaa aaatcatggc aggcaacact ttcggacaac tcttcaccgt
     3841 taccaccttc ggcgaaagcc acggcgcggg tttgggctgt atcatcgacg gctgcccgcc
     3901 cggcttggaa ttaagcgaag cggatatcca atttgacctc gaccgacgca aacccggcac
     3961 cagccgccac gttacccaac gccgcgaagc cgaccaagtc gaaatcctct ccggcgtatt
     4021 cgaaggcaaa accaccggca cgcccatcgc cctcttaatc cgcaataccg accagcgcag
     4081 caaagactac ggcaacatcg ccaccagctt ccgccccggc cacgccgact atacctattg
     4141 gcacaaatac ggcacgcgcg actaccgcgg cggcggcagg agctccgccc gtgaaaccgc
     4201 cgcccgcgtt gctgccggag ccgttgccaa aaaatggttg aaagaaaaat tcggcacgga
     4261 aatcaccgcc tacgttaccc aagtcggcga aaaagaaatc cggtttgaag gctgcgaaca
     4321 catttcccaa aatccttttt ttgccgccaa ccatagccaa attgccgagc tggaaaacta
     4381 tatggacggc gtgcgcaaat ccttggattc cgtcggcgcg aagctgcata tcgaagcagc
     4441 caatgtccct gtcggcttgg gcgaacctgt ttttgaccgc ctcgatgccg aaatcgccta
     4501 cgcgatgatg ggcatcaacg ccgtcaaagg cgtggaaatc ggcgcaggtt ttgacagcgt
     4561 aacgcaacgc ggcagcgaac acggcgacga actgaccccg caaggcttcc tgtccaacca
     4621 ctcaggcggc atcctcggcg gcatcagcac cgggcaagac atccgcgtca atatcgccat
     4681 caaacccacc agctccatcg ccaccccgcg ccgcagtatc gacatcaacg gcaaccccat
     4741 cgaactcgcc acgcacggca ggcacgaccc ctgcgtcgga ctgcgctccg cgccgatcgc
     4801 cgaagccatg ctcgcgttag tcctcatcga ccacgccctg cgccatcgcg cgcaaaatgc
     4861 cgacgttcag gttaatacgc ccgacattac cctttcaaac aaataaaaat ttagccaaaa
     4921 cacagacttt ataatagaat atcgagcatt gccgtgcagc cttgacgcac gggtttgttt
     4981 agaggaatac aaccgaaatg acacaagaaa ccgctttggg cgcggcacta aaatccgccg
     5041 tccaaactat gagcaaaaag aaacagaccg aaatgatcgc cgaccacatc tacggcaaat
     5101 acgatgtgtt caaacgcttc aaaccgttgg cgctcggcat cgatcaggat ttgattgccg
     5161 ctttgccgca atacgatgcc gcactgattg cacgcgtcct cgccaaccac tgccgccgtc
     5221 cgcgctatct gaaagccttg gcgcgcggcg gcaaacgttt cgatttgaac aaccgtttca
     5281 aaggcgaagt tacccccgaa gaacaggcga tcgcgcaaaa ccatcctttt gtgcagcagg
     5341 ctttgcaaca gcagtccgcc caagctgccg ccgaaacgct gtctgttgaa gccgaagcag
     5401 ccgaatcttc cgcagcagaa taaatcccca aacgaaatgc cgtctgaaaa ccgatttggt
     5461 ttcagacggc attttttcgt atgcggcaat cacggttcaa atatcgagtt ccgccgtatc
     5521 gccttcgcgt tccatccagg cacggcgggc ggcggcttcg cctttgccca tcagtttgac
     5581 gaagatgtcg cgcgtctcgt catccgcgcc ttctgggatt tgtacctgca acaggcggcg
     5641 ggtgtcgggg tgcatggtag tatctttgag ctggtctggg ttcatctcac ccaagccttt
     5701 gaaacggctg atggaataag cagtttcttt aacgccttct ttttgcagcc gctctaaaat
     5761 gccgtcaagt tcgttttggt cgagggcgta gaatttgcgg gcgggtttgc ttttaccttg
     5821 tgcgttgaca tctacgcgga acagcggcgg ctgggcgacg tagatgtgtc cgtcagcgac
     5881 cagtttcggg aagtggcggt agaacagggt cagcagcaaa acttgaatat gcgagccgtc
     5941 cacgtcggca tcggacagga tggcgatttt gccgtaacgc aggccgctta aatcgggatg
     6001 gtcgttgatg ccgtgcggat cgacgccgat ggcaactgaa atgtcgtgga tttcggcgtt
     6061 gccgaagagc tggtcggggt ggacttcaaa gctgttgagc actttgccgc gcaggggcag
     6121 gatggcctgg gtggctttgt cgcgggcgag tttggctgag ccgccggcgg aatcgccttc
     6181 gacaaggaag agttcgtttt cgcggatgtc ttcgctttcg cagtcggtca gcttgccggg
     6241 caggacggcg acgccgctgc cttttttctt ttcgattttc ttaaccgaac gcatccgcgc
     6301 ctgcgcctga cggatggcga gttcggcaat ttttttaccg aagtccacgt tttggttcag
     6361 ccacaattcc aaagggtcgc ccgctacagc ggcgacaagt ttcagcgcgt cgcggttggt
     6421 cagcttgtct ttggtctgac cttggaactg cgggtcgagg acacgggcag agaggacaaa
     6481 ggcggttttg ccgaacacgt cgtcgctttg tactttcacg ccgcgcggca agaggttgtg
     6541 cagattgatg aaattgttga cggcgttgaa cacggcttgt ttcaagcctg cttcatgcgt
     6601 gccgcccaaa ggggtgggga tgaggttgac atagctttcg ttggcacacg agccttcttc
     6661 cagccaagtc agggcaaacg ccgctccttc gccgatgctg aaatcgccgt tgtgttcgtc
     6721 tgaaatgtag ttttcgcaag agaacagcgg cacagcttct tgcgcgtcgg caatcaggtc
     6781 ggtcagatag cttttcaggc cgtcggggta gtgccaggtt tgggtgtgcg cttcgtcttc
     6841 gcctttgacc ggacgggtca gggaaacgcg cacacccggc agcagcacgg ctttggcacg
     6901 cagcaggcgt tcgagttcgg gaatgctgta attcgggctt tcaaaatatt tgccgtccgg
     6961 ccagacgcgc acttcggtgc cgctgtcttt gacggcgcat ttgcccactt gtgccaacgg
     7021 ttcgaccacg tcgccgccgg caaacacgat acggtggatt ttgccttcac gtttgaccgt
     7081 tacctcaagg cgggtggaga gggcgttggt gacggatacg cccacgccgt gcagtccgcc
     7141 tgaaaaggca tacgcgctgc cgccgtcttt tttgttgaac ttgccgcccg cgtgcagacg
     7201 ggtgaacacg agttcgacta cgggtacgcc ttcttcaggg tgcaggccga cgggaatacc
     7261 gcggccgtta tcacggacgg aaagcgaacc gtcttcatga atttgcacgt cgattgcagt
     7321 cgcgaaacca cccaacgcct cgtccgccgc attatcaatc acttcctgac agatgtgggt
     7381 cgggctgtcg gtgcgggtgt acatgccggg acgttctttg accggctcca agcctttgag
     7441 gacggtgatg ctggattcgc tgtattggtt gtttttagcc ataggaataa tctgaaagta
     7501 agaaaaacaa cgctttcaga cggcctgaaa gcgttgcgtt ccgttgtttt agcggttgtc
     7561 ggaagattgg cgggcggcaa agtcttcata actttccata ccgcgcagga agcgggaaga
     7621 gagttctttc aacgccccca aataaacgtc gcgtttgaaa tcaatcactt cgtcaacgga
     7681 cgcccaatat tgatgccaac gccagccgtc aaattcgggg tggcgggtgg cgcgcaggtt
     7741 gacatcgcaa tctcggccgg tcaggcgcag gagataccaa atctgcttct gcccgcgata
     7801 ggatccgcgc cattcgcggc gtacccagtt gttcggcacg tcataacgca gccagtcgcg
     7861 cgtgcggccg ataattttga cgtgttgcgg caaaagcccg acttcttcgt acaactcgcg
     7921 gtacatggcg gtttcggggc tttcgcccgg cttgatgccg ccttgcggaa actgccaaga
     7981 atgttcgcgc acgcgcttgc cccaaaagac ttcgtcgcgg ttgttgatta agatgatacc
     8041 gacattgggg cgatagcctt ccctgtccaa cacggtgtcg ccctccgtta aattcaatct
     8101 tgggattttc ccacaaatcg gacggttttg acaaatcgga cgatagggtt caggcatgtc
     8161 gaaacacaga cggattcggg aaaatatctt aaatttggtt tacaataata cgtttcaaat
     8221 taattcggga atcagaccat gttagatatc caattgctcc gcagcaacac cgccgctgtg
     8281 gcggaacgct tggcggcacg cggttacaag tttgatgccg cgcgtttcaa tgcgctggaa
     8341 gagcagcgaa aagccgtcca ggtgaaaacc gaagagctgc aagcctcgcg caacagcatt
     8401 tccaaacaaa tcggcgcact gaaaggtcag ggcaaacatg aagaggcgca ggcggccatg
     8461 gatcaagtcg cccaaatcaa aaccgatttg gaacaggcag ccgccgattt ggaagccgtg
     8521 caaaaggaac tggacgcatg gctgttgagc attccgaacc tgccgcatga ggacgtgcct
     8581 ttcggtaagg atgaaaccga aaacgtcgaa gtccgcaaag tcggcacgcc acgcgaattt
     8641 gactttgaaa tcaaagacca tgtcgatttg ggcgaacctt tgggtttgga tttcgaaggt
     8701 ggcgcgaaac tttccggcgc acgatttacc gtgatgcgcg gacaaatcgc ccgcctgcac
     8761 cgcgcgctgg cgcaattcat gctcgatacg cacacgctga aacacggcta caccgagcat
     8821 tacacaccct atatcgtgga cgacaccacc ttgcaaggca cgggtcagct gccgaagttt
     8881 gcagaagatt tgttccacgt tacccgtggc ggcgacgaga gcaaaaccac gcaatatctg
     8941 attccgacag ctgaagttac cctgaccaat accgttgccg gcagcattat cccgtccgaa
     9001 caactgccgc tgaagctgac cgcgcattcg ccctgtttcc gcagcgaggc gggttcgtac
     9061 ggcaaagaca cgcgcggtct gattcgccag caccagttcg acaaagtgga aatggttcaa
     9121 atcgttcatc ccgaaaaatc atacgaaacg ctggaagaaa tggtcggcca tgccgaaaac
     9181 atcctgaagg ctttggaact gccctaccgc gtgattaccc tgtgtaccgg cgacatgggc
     9241 ttcggcgcaa ccaaaacgta tgacttggaa gtttgggtac cggcgcaaaa cacctaccgc
     9301 gaaatctcaa gctgctccaa ctgcgaagat ttccaagccc gccgcatgaa ggcgcgtttc
     9361 aaagacgaaa acggcaaaaa ccgcttggta catactttga acggctccgg cttggctgtc
     9421 ggcagaacgc tggtcgccgt attggaaaac catcaaaacg ccgacggcag catcaacatc
     9481 cctgccgcac tgcaaccgta tatgggcggc gttgccaagt tggaagtgaa ataagtttgc
     9541 aagctgcctg aacgtcaaat gccgtctgaa acctggttca gacggcattt cctttaaact
     9601 tttaaaacac atcaaccgtc ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc
     9661 tcgcggatat tggacagcgt aacttccgaa atattaccca acgcctcttc cgtcaaaaat
     9721 gcctgatggc cggtaaacag cacattatga caagacgaca ggcggcggaa cacgtcgtcg
     9781 gtaatcacat cgttggattt gtcttcaaaa aacaattcgc gttcgttctc gtacacgtcc
     9841 atacccaatg cgccgatttt ccggcgtttc aacgcctcaa tcgcggcaga actgtcaatc
     9901 agaccgccac ggctggtgtt gataatcatc acgccgtctt tcattttgtc gaacgccgct
     9961 tcgttcagca tatagtggtt ttccggcgtg gaggggcaat gcagcgtgat gatgtccgac
    10021 cgggcataca gctcgttcaa ctccacatac ctgccgccga ttttttccgc ttcggggttg
    10081 caaaacggat cgtaagccag caggttcatg ccgaaaccct ttaaaatccg catggttgca
    10141 ataccgattt tccccgtgcc gataacgccc gccgttttgc cgtacatatt gaaaccggtc
    10201 agaccttcca gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg
    10261 ttcaacgtca gcatcagacc gaccgtatgt tccgcaaccg attcgggcga ataggcaggc
    10321 acgcgcacga ctttcaagcc caactcttca gccgccttta aatccacatt attgaagccg
    10381 gcacaacgca acgccacagt tttcacgcca atttgcgcca atttttccaa cacgggccgg
    10441 ctgccgtcgt cgtttacaaa aatacagacc gcttccgcgc cttccgccat tttcgccgtt
    10501 ttcgcatcca gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg
    10561 gtaaaatgtt cgcggtcata gcttttcgta ccgtaaatcg caatcttcat caatatgtcc
    10621 agttgtcgcc tatggttgag aaacggcata atacactgaa ttcaaacaaa tcagtagaat
    10681 atggcagatt aaaattgatt gcatacacgg catttccatt tcaaaacaca aaactcaatc
    10741 gcccattgcc gccagaagct ccgcctgatg ctcggcaatc agggcattgg tgatttctgc
    10801 caagtcgccg tccatcacaa aatccagctt gtgcagggta aggttgatgc ggtggtcggt
    10861 taccctgcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat
    10921 gagggatttg cgctcggcgg cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag
    10981 gcgggcggcg aggactttca ttgcctgcgc tttgttggca tgttggctgc ggccgtcttg
    11041 gcattcgacc accatgccgg tgggcaggtg ggtgatgcgg acggcggagt cggttttgtt
    11101 gatgtgctga ccgcccgcgc cggatgcgcg gaaggtgtcg atgcgcaggt cggctgggtt
    11161 cagttcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt
    11221 gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc
    11281 aaatttcaaa cggctgtacg ccccgagtcc gacaatacgg gcgatgactt ctttatagcc
    11341 gcccaattcg ctttcgttgg cggacacgat ttcaacctgc caacggttgc gctcggcgta
    11401 gcggctgtac atacgcagca aatcgccggc aaacagcgcg gcttcgtcgc cgcccgttcc
    11461 ggcgcgtatt tcgatgaaga tgtttttgtc gtcgtcggca tctttgggca gcagcagttt
    11521 ttgcagttcg gtatcgagcg tgttgatttt ggcttttgcc gcttcgattt cttcggcggc
    11581 aaaatctttc atttcggggt cggacaacat ttcttcggca tccgccaagt cgctttgggc
    11641 aagccgatag ttttggaaca cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag
    11701 cttgcggtag ttgtccatat ctgccgtggc ttcgggttgc cctaaaaggt gggtaacttc
    11761 ttccaaccgg tcgccgagtt gttgcagttt ttctaagata gacggcttca taattcttcc
    11821 ataacaaacg ccgcctgaat ttcagacggc atcaaaacgc ggtcattata atatattttc
    11881 tgaatattca aaaagataat ctagggacgg agcaggctgt cggcattgcg ttccgttatc
    11941 gcggcgatgt ccgccacgcc gacaccgcgt atctgcgcgg caatttcggc aatccaaaga
    12001 atattggcag gcgtattaat ctcttttttc agcataaacg ggctgtcggt ttccaatacg
    12061 aaatccccgt cgttcaaggc tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc
    12121 aacaacgaac cgatgccgat tttgaaaccc agtttcgtca acacgcgcgc ttcttccgcg
    12181 ctgcctgaga aggcgtgaac gatgccgcct tgggcaaagc ctgtctgttt gacggcggcg
    12241 gcgatgtcgg cggtggcttt gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg
    12301 atggcaagct ggcggacgaa aacttggatt tgccgttcgc gctgcggcgg cgtttgggtt
    12361 ttgtcgtaaa aatccaaacc gatttcgccg acccatgcct gcggataacg tgccaacatc
    12421 gcttccaggc ggacgcaatc acgctcggca atgccgtctg aaaaccaagg atggatgccg
    12481 agcgcaatac ggatttgacc gtgttcggac ggcatttccg acagttccgc cacgctttgc
    12541 caatcctgcg ggcgcgtcgc gggaacgata aaccgccaca cacccgcctg tcgcgcttcg
    12601 gcaagcacct gcgccagatt gccgcgcaaa acagaatcgg caagatggca atgtgtgtcg
    12661 gtcagcctca tcttcacacc agccccaacc gctgcaactc gtgtttcacc tcgtccgcgc
    12721 cccatccttc agaaacggca agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc
    12781 cgccgttgat gatgcccgag ttgcggaacg cgttgccttg cgcgtaaacg gcggcggttt
    12841 tgccttgtcg gacttggctg atgttgagca gcagtttgcc ctgccgcgcg aagtctcgga
    12901 cggcgcggat aaaaccttcg tctgcgggcg tgttgccgtg tccgtagctt tgcaggataa
    12961 gggcttgggc gggaaactgt ccgagtccgt cggcaagttc ttggacggca aagccgggga
    13021 taagcgtgcg gacggcgatt tttgcctgcg ggtcaggata gcggattttg aggccgtctg
    13081 aaacggccgc cgcatcttgg gacggaatac gaagattgta ccagccttga gtttcatccc
    13141 attcggcgat gacaccgaaa tgcggattat cgaagcctga ggcggtttcg gtgctgactt
    13201 tgctgctgcc gacggcggga tacagtttgc cgtcaaacgc gatgacggtt tgtttgagct
    13261 tgaggctgaa ggcggcaacg gcggtggaga ggttgcgcgg ggcatcgctg ttttcggcgg
    13321 cgtaaggcca ttgggaacca gtcaggacaa tcggtttgcc caaaccttgc agagcgagcg
    13381 cgaggagatt ggcggtgtac gccatgctgt ccgtgccgtg cagtatcagg atgccgtcgc
    13441 atgaagggag tttgtcggca atgatgtcca gccaatcgcg ccagtgttgc agcgtaacgg
    13501 aggaggaatc aatcaaggga ttgcagacgt gccactcgaa atcgaggccg tctgaaaagg
    13561 gggaaagggc ttggctaacc agtgcggtat cggggcgcag gccttcgctg ctttgggtca
    13621 tgcctatggt gccgcctgtg tagaggacga agattttttg tttcatggac atcatcgggt
    13681 cgtctgaaaa taataatacg gcttatttaa ctatatttcg gacagactgg caatttggcg
    13741 gcgcggacgg ttttcagacg gccttcaaat gaaaaagcac ccgagggctg tcgatatttg
    13801 attttccaag tagattttta ttcacgaaat aggagagccg caacaagctt aaatcccttg
    13861 tgaggttccc aacacggaag ataccgcttt gtggattaaa aaatacggaa actattgaat
    13921 atcgacaacc tatttaggtg cttgatttta ttgtttgctt tgcgcggctt ttttggctgc
    13981 cttggcggct ttgcgttgcg cccgcttttc tttcaatttg ctgcggtaaa actggatacg
    14041 ttggcgtttt ttccaccaaa tccaagcgac aacggtcgca cctataccca agataacaaa
    14101 aatacccgat tgcaggctgt gcattttcgc catcagccaa tcgatgttgt gcgcaccgta
    14161 ttcgcccaga taaatccaaa tagggacgga aatcagtgcg gccagtccat ccataatgat
    14221 aaaacgcaag tatgaaacct tgcggctgat accggctgta acaaatacgg ccgttctcaa
    14281 accgggcagg aaacgggcga caaataagac ccagttaccg tatttgtcga atttttcctg
    14341 aacctgctca taacgtttcg gcgtcatgat gcgcgcaatc ggtttgaacc ttaggatttt
    14401 ctgcccccaa attcgtccgg cggcgaacat gatgccgtcc ccgaccaata cgccgagcat
    14461 accgactgca aacataatat gcggattggt ataacccata cccgaaatca cgccgcctgt
    14521 taccaaggtc aaatcctcgg gaatcggcac gccgaaaccg cagatgacca aaacgaaaaa
    14581 cacggccgca tagccgtatt cgacaaaaaa ggcttccaaa agggcaaaca tagcggatat
    14641 tccatcgtcg ggggataaaa agtcagaaca agccgaaaca ttttctgcgc gaaacggcat
    14701 tctatcaaag attatgccgt ctgaaagcgg caaggggcag attcgcgccc tctgccggac
    14761 gttcagacgg catttaaaca ggaaaaccgc cgctgttgaa acggttttgc ctgattttgc
    14821 ctaaatgccg ccgatggcgg cggcaatgcg ttccgcccct tcgcgcgccc aatccgcctg
    14881 ccgcgcctcc accatcacgc gcacgacggg ttcggttccc gaagcgcgca acacaaccct
    14941 tcctttgcct tcgagttctt tttccacttc cgccaacacg tcttttgagg cttcctgcca
    15001 gttttgccct ttgccgatac ggacattaat catcgtttgc ggatacggct gccagtcggc
    15061 gcaaacggtg gcgaggtctt ggttcaggat ttgcagcgcc gccaaaactt gcagcgcgga
    15121 aataatgccg tcgccggtgt tgtgtttgtc catgcacaaa atatggccgc tggcttcgcc
    15181 gccgatgagc caaccgcgtt ggttcagctg ttccaacaca tagcggtcgc cgactttggc
    15241 gcggcagaaa tcgacgccct gctctttcag ggcgatttcc atcgccatat tggtcatgac
    15301 cgtgccgacc acgccgccga tgttgatacc ttctctggcg cgggctttag caatgacgta
    15361 aatcaggctg tcgccgtcgt aaaccttacc gtttttatcg accatcatca ggcggtctcc
    15421 gtcgccgtct aaggcgatgc cgtagtcggc ttcatgctgt aaaacggcag cctggagtgt
    15481 cttggtataa gtcgcaccgc atttttcgtt gatgttgtag ccgttgggtt cgtcgccgat
    15541 gctgacgact tgtgcgccca gttcgtgaaa cactttgggt gcgacgctgt aagccgcgcc
    15601 gttggcggca tcgacgacca gcttcagccc gcgcaaatcg gaatgcgtag ggaaggtgga
    15661 tttgcaaaat tcgatatagc ggtcgtccgc gccgttgatg cggcgtgcgc gaccgagacg
    15721 ggcggacggt tgggttttca tttcgccgtc gattttggct tcgatttcca actcgacttc
    15781 atcggaaagt ttcacgccgc cttcggcgaa gaatttgatg ccgttgtcgg aataggcgtt
    15841 gtgcgacgcg gaaatcatca cgccggcgga caggcgcaac gcgcgggtca gataagccac
    15901 gccgggcgtg ggcagcggtc cggtctgtac cacattcaca cccgccgccg taaaaccggc
    15961 caccaaagcg gcttccagca tatagccgga aatgcgcgtg tctttgccga tgaggacggt
    16021 cggtttctgg tcggtgtcgt gctgcaccaa aacctgcccc gccgcatagc cgagtttcaa
    16081 tacgaaatcg ggcgtaatcg gaaattgccc cacttcgccg cgcacgccgt ccgtgccgaa
    16141 atattttttt gccatgtgtt gctccgagaa tgtggaccgt tgtccgagat tatacagtca
    16201 gtttgtgcct tgctgtctgc accgttgatg ccgtctgaaa ccaacccgcc cttttcagac
    16261 ggcatgaagt atgcgaaccg ctgtttacag attgatgccc aacgcttccc acaccttcaa
    16321 cgcgtccgcc gtcgccttca cgtcatgcac ccgcacaatt tgcgcgccgc gcgccacagc
    16381 cgccacagcc gccgccacgc tgccgtgtac gcgcgccgcc gcgtcggttt cgcccgtcag
    16441 ttcgcctacc atgcgtttgc gcgatacgcc gatcagcagc ggaaagcctg tttccgccat
    16501 caattcgggc aaatgcctca tcagcgcgat attgtgttgc agggttttgc cgaagccgaa
    16561 acccggatcg agcgtgatgc gttgcggtgc gatgcctgcc gcgatgcatt ccgccgcccg
    16621 cgctttcaaa taccgcgcca cttcaccgac aacatcctga tatttcggat taatctgcat
    16681 gttttcaggc aaaccccgca tgtgcatcag gcaaatgccc gtgtccgtct ggcgcgccag
    16741 caattcgacc gcaccttcgt cggtcaacgc cgccacatcg ttaataatat cgatgccacc
    16801 gagtgccaac gctttttcca taatcaccgt gcggcgcgtg tccaaactga cgggaacgcc
    16861 ccaccccgcc acttctgcca atacaggctc aacccgcgcc cattcttctt caggcgaaac
    16921 ataatccgcg cacggccgcg tcgattcgcc gccgatgtcg agaatgtccg caccctcttt
    16981 caaaagccgt tcggcatgcg ccaaagcggt ttgggcgttt tgagaatacg cgccgccgtc
    17041 ggaaaaagaa tcgggcgtga gattcacgat acccatgatt ttcggtttgt ccaaaccgat
    17101 ttcaaaccgt cctgcctgcc aaacgtgtcg tgccatctga actcctccca aaataaaaaa
    17161 cagattatat gccgtctgaa accgtcttgt gcgcttcaga cggcaccgct attcgggcgg
    17221 cagacggcat gttgtccgaa tgtctgctcc gcctttgaat ctgccggtat gcctgctatc
    17281 cgcccgactt ttcaaaacag gttccgacga ttccgcacgc gcctgccgcc tttgccaagc
    17341 cgtacaggat ttcctgcggc atatcgcggt tccataatcc cgtaatattc gcaatcacgg
    17401 gcagatggct gatttggcgg actttcacga tggattcgac atccaaacgg tagggatggt
    17461 ctttggtatg gctcaacacg cccgactcgc ccagaatcag gtggcggttg ccgcgcgaga
    17521 cgacatattc tgcggcattc aaccaatctt cggcagaatg gtgcttgtct ttacacagca
    17581 ccaagggaat gttcaggctt ccggcttcat tcaatacggc aagatcggac atcagcccgc
    17641 cgcccaaata caggatgtcc gcccccgcat tcaaagccgc ttcgacatgg cggacgttgc
    17701 ggacgcgcac caatacgggt ttccctgcat catgcgccga tgcggtctgt tccgccaacc
    17761 gtctgcaccg tccccgccct tcatccgcac ttgaagtgtc gtataagttt gccgaagtga
    17821 aaaacggatc tagaaacact gcatccgcat tgcgccatac tgacggttct gcggcgatac
    17881 ggacggtttc cccgccgccg aaagccacgc ctttggcggc aacgcggctg tcttccgccc
    17941 gattttcccg actgacggtt ttccatgtat ccaaaatgcg gacggctttc tcgacctccg
    18001 gcagcgtctg cacctccctg acgctcaaaa ccctatcgtc gccgattgcg ccgatgacgg
    18061 tacgctcgtc gccgtgagaa atgtgttctc gcagacctct gctgcggata aaggcgacaa
    18121 cgccggcaat gtccgcttcg gcggcacgcc tgctcatgac aataatcata tttcctcctg
    18181 acacaagaaa cggcctgccc aaaataggat ttttgcaagc cgtgttatac tgtggcgtgt
    18241 tttacagatt gttcgggcta tggatttatt atcggttttc cacaaatacc gtctgaaata
    18301 tgtggtagcg gtgctgacga tactgctttt ggcggcaatc gggctgcacg cttccgtcta
    18361 tcgcatcttc acacctgaaa acatccgcag ccgcctccaa caaagcattg cacacacaca
    18421 ccggaaaatc tcgtttgatg cggacattca gcgcaggctc ctgccccggc cgaccgtcat
    18481 cctgaaaaac ctgaccatta ccgaacccgg cggcgaccgg actgccgttt ccgtccaaga
    18541 aaccaaaatc ggattgagct ggaaaaacct gtggtcggat cagatacaga ttgaaaaatg
    18601 ggtggtttcg agtgcggaac ttgccctgac gcgcgacggg aaaggtgttt ggaacattca
    18661 agacctgatc gacagccaaa aacgccaagc ctcagtcaac cgcattatcg tcgaaaacag
    18721 caccgtccgc ctcaatttcc tgcaggaaca gcttatcctg aaggaaatca acctcaacct
    18781 gcaatccccc gattcgtcgg ggcagccgtt tgaaagttcg ggcatactgg tttggggaaa
    18841 gctgtccgtc ccgtggaaaa gcagggggct gttcctttca aacggcatcg gcccgcccga
    18901 aatctcaccg ttccattttg aagcttccac ttcgctggac ggacacggca ttaccatttc
    18961 caccaccggc agcccttctg tccgcttcaa cgccggcgga gcggatgccg ccggcctcgg
    19021 cctgcgtgca gacacttcct tccgtaacct ccacctgacc gcccaaatcc ctacgctggc
    19081 actcaggaac aacagcatta aaattgaaac cgtcaacggc gcatttaccg ccggcggcga
    19141 atatgcccga tgggacggtt cgttcaaact cgacaaagcc aacctgcact ccggcatcgc
    19201 caacatcggc aacgccgaaa tctccggcag cttcaaaaca ccgcgccacc agaccaactt
    19261 ctccctcaat tcgccgctcg tatggacgga aaacaaaggg ctggacgcgc cgcgcctcta
    19321 tgtatcgacc cttcaggata ccgtcaaccg cctgccgcaa ccccgtttca tcagccggct
    19381 cgacggttcg ctgtccgtac cgaatctgca aaattggaat gccgaattaa acggcacatt
    19441 cgaccgccaa accgttgccg cgaaattcag atacacacat gaagacacac cgcatctgga
    19501 agccgccgtc gcactgcaaa aattgaacct gaccccctat cttgacgacg tgcggcaaca
    19561 aaacggcaaa atatttcccg acaccctcgc caagctgtcc ggcgacatcg aggcgcacct
    19621 gaaaatcgga aaagtccaac ttcccggcct gcaactggac gatatggaaa cctacctcca
    19681 cgccgacaaa ggccatatcg cgctcagccg tttcaagtca gggctttacg gcggccatac
    19741 cgaaggcggc atcagcatcg ccaacacccg tcccgccact taccgcctgc aacagaatgc
    19801 aagcaacatc caaatccaac cgctgctgca agacctgttc ggcttccaca gcttcagcgg
    19861 caacggcgac gcggtcatcg acctgaccgc gggcggcgaa acccgaaaag agcttatccg
    19921 ctcgcttcag ggcagcctgt cgctaaatat ttccaacggt gcatggcacg gtatcgacat
    19981 ggacaatatc ctgaaaaacg gcatttcggg caaaactgcc gacaatgccg cacccagcac
    20041 acccttccac cgattcacgc tcaacagcga aatttcagac ggcatcagcc gccacatcga
    20101 taccgaactc ttctccgaca gcctctatgt taccagcaac ggctatacca atctggatac
    20161 gcaggaattg tctgaagatg tccttatccg caacgccgtc catccgaaaa acaaaccgat
    20221 tcccctgaaa atcaccggta cggtggacaa gccgtccatt accgtcgatt acggcaggct
    20281 gaccggcggc atcaattcgc gcaaagagaa acagaaaatc ctcgaagaca ccctgctgga
    20341 acaatggcag tggctcaaac ctaaagaacc gtaaacatcc tgcgtacaaa aatgccgtct
    20401 gaaacgcccc cgcgcttcag acggcagacc gtaaagccta caaccccaat tcctcccaaa
    20461 tcccatcaat cttagccgta accgcagggt cttttttgat gacgcgtccc cattcgcggt
    20521 cggtttctcc cggccatttg ttggtcgcat ccaaacccat tttgccgccg agtccgctga
    20581 cggggctggc gaagtcgaga taatcgatgg gcgtgttttc taccaaaaca gtgtcgcgca
    20641 cggggtccat gcgcgtggtg accgcccaga tgacttcttt ccagtcgcgc acgttcacat
    20701 cgtcatccac cacgataatg aatttggtat acataaactg gcgcaggaac gaccagcagc
    20761 ccatcatcac gcgtttggcg tgtccggcgt actgtttttt catgctcacc accgccatgc
    20821 ggtaggagca gccttcgggc ggcaggtaga aatcggtgat ttcgggaaac tgcttttgca
    20881 agagcggtac gaacacttcg ttcaacgcca cgcccaaaac ggcaggctca tcgggcggtt
    20941 tgcctgtgta ggtcgaatgg taaatcgggt tttcgcgcat ggtgatgcgt tcgaccgtaa
    21001 acacagggaa ataatcctgc tcgttgtaat agcccgtgtg gtcgccgtac gggccttcca
    21061 acgcggtttc gttcggatga atcacacctt ccagcacgat ttctgcgcgt gcaggcactt
    21121 gcaaatcgtt gccgatacat ttcaccagct ccgtccgcga accgcgcagc agtccggcaa
    21181 actggtattc gctcaaggta tcgggaacgg gcgttaccgc gcccaaaatg gtggcagggt
    21241 cgcagccgag cacgacggcg acgggatacg gcgtatcggg attgagtttg cggaattcct
    21301 gataatccaa cgcgccgccg cgatgcgaca gccagcgcat aatcagcttg tttttgccga
    21361 tgagttgttg gcggtaaatg ccgagatttt ggcgtttttt gtgcggcccg cgcgttacgg
    21421 tcaagcccca tgttaccagc ggcgcaacgt cttccggcca gcaatgctga atcggaagtt
    21481 tatacaaatc aacgttttcg ccttcccaca cgatttcctg acacggcgcg tttttcacca
    21541 cgttcggcgc catgctccaa atgtctttca gtagcggcag tttggaaaac gcatctttga
    21601 tgcctttggg cggttcgggt tctttcaaat acgccagcgt ctgcccgatt tcacgcagct
    21661 tggacacgct gtccgcgccc atgcccatcg ccacacgttc gggcgtgccg aacaggtttg
    21721 ccaacacagg ataaccgtag cgcgtaccgt cgggcttaac cgggttttca aacagcaacg
    21781 ccggcccttc ggcgcgcagc acgcggtcgg cgatttcggt catttccaaa tgcggggaaa
    21841 cggggtgcgc gatgcgtttg agtttgccct gctgctcgag catggcgatg aagtcgcgca
    21901 ggtctttgta tttcatattc atcctttttg tccttttatc ctgagcaatc cgattcggat
    21961 accgccccta tccttgcctg tgcctcggca tattctatgc cgtgataaaa gtcgcgtacc
    22021 agcggatgtt cgctgccttg atggagttgc agcaaagggc gttgaccatc gggttgggta
    22081 acgacattgc aatgcatacc gaaggtgtcg gattcgtaag ggggcagccg gttgcagatc
    22141 atcccgaaat aaacgacatt ttccggattg tcgtgaaagt ggctttgata gtcgttaaaa
    22201 ctcttttcgc tgacggacac ccacacgcca tattccagcg tttcctcatg cccgataatc
    22261 ggaatcggca gcaccgcgcg gataaagcgg tcggtttggt cgggatagcg gatgatgcag
    22321 aaatcagaat cgcattctgc ttgataagca atgcgttctt cttcactgag ttgattatag
    22381 ggatcggggg cggtaaagcc gattgcgggc atatcttcat gtttttcgcc gcaggaagtg
    22441 caagtgtaca taaggtttca gactttcaaa acgagtttgc ggtaaagcca ttcgccggca
    22501 aacagcatcc ccatcaatac ataggcaatc acgcctgtat aaaccgccca ccaatcatat
    22561 cgccccaacc gtgccaacaa agcggcaagc gtcccgttga ttataaaaaa tacgcaccaa
    22621 acctgcgtta cccggcgggt atagcgcacg gctttttcag gcaggtcggg ctgttgcagc
    22681 cgcgcaagtt tttcaatcac cgtctgccct gcaaacaggc tgccgccgaa caccgccaac
    22741 atcatcagat tgacgaggac gggataccaa tacatcgaat catgccgccc gaacaccaat
    22801 actgcggcaa aaaacagtgt aataaacaaa gccgcataac gctgttgggg cagtttggca
    22861 gtcagggcgc gcagcagcca caacccgcac atcgccgccg ccagccagac aaaccagccc
    22921 gcctccctgc cgtaatatag tggattaaca aaaaccagta cagcgttgcc tcgccttgcc
    22981 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat accacaaagc
    23041 gggataggca atgcttaata cggtcagaaa aatatgtccg aaaaaaccgg gtttcatttt
    23101 gaatccgcac aaatgttttc agacggcatc cgataaaaac atgccgtctg aaaaataatt
    23161 agaaataccc gattagcccg cctgaatctt caataccgcc tgtaccacgt cgttgacggt
    23221 gcggacattg cggaaatctt cagcctgcag cttgcgggct gccacagcca ttcacgattg
    23281 cgatataccc ggcagcattc ccgactgccg ttcagcagaa agcgcaccca ttccaaaccg
    23341 ctccaatatg cctcgggcag cataccggct tcagacggca tatcgtccga agcagataaa
    23401 gtcaggctgt aacgcgtccc tttggtcaga accatcgcca aagcataagc aaacggcgcg
    23461 cgcgttgccg atacggcata tccttccggc agcggatcgt ccgccgccaa aaccaaaacc
    23521 gacccgcatc cctcttccaa cagtgatgcc gcttccgcca atgccgtttc cacaccgtcg
    23581 gcacacacgg ccaatgccgt ctgctcgctc atatcccgcc tcaatatcga ccattacccc
    23641 gccgttgcat tgtgcaccga caaaccgaac gaagtcggcg acacgatttg cgatttcaac
    23701 agttccaacc acaaatcgaa actgcgcgcc atttccccgt cgtgcgaggc ataaactata
    23761 gtggattaaa tt
//
LOCUS       DAWGJV010000011        23226 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWGJV010000011 DAWGJV010000000
VERSION     DAWGJV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.011"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(212..4381)
                     /locus_tag="WHQ94_000324"
     CDS             complement(212..4381)
                     /locus_tag="WHQ94_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ2127671.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLSWLAGTESGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISAGKAFDKPTVYLERLDASYRYDIKGHRLDLKAADTPWSNSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGNFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNVSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGIENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4436..6283)
                     /locus_tag="WHQ94_000325"
     CDS             complement(4436..6283)
                     /locus_tag="WHQ94_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ2127672.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6696..7925
                     /gene="sstT"
                     /locus_tag="WHQ94_000326"
     CDS             6696..7925
                     /gene="sstT"
                     /locus_tag="WHQ94_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ2127673.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8037..9458
                     /locus_tag="WHQ94_000327"
     CDS             8037..9458
                     /locus_tag="WHQ94_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ2127674.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEVKEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAATADNPKNEDEGAQDDM
                     PQKAAGTDSSTPNHTPAPNMPAGNMENQPDMANTADRMQGDDPSAGENAGNTAAQGAN
                     QAENNQAAGSSDSTPASNPAPTNGGGDFGRTNVANGVLIDGPSQNITLTHCKGDSCSG
                     DNLLDEEAPSKSEFEKLSDADKINNYKKDGKNNNKFVGLVADRVKKDGTNKYIIFYTD
                     NPPTRSARSRRSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT
                     YGAEKLSGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPSPFRGRFAAKVDF
                     VSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGK
                     YSYRPTDAEKGGFGVFAGKKGQD"
     gene            complement(9533..9748)
                     /locus_tag="WHQ94_000328"
     CDS             complement(9533..9748)
                     /locus_tag="WHQ94_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127675.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9741..9956)
                     /locus_tag="WHQ94_000329"
     CDS             complement(9741..9956)
                     /locus_tag="WHQ94_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ2127676.1"
                     /translation="MKEYKVVIYQESLLSSLFFGTAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10031..11374)
                     /gene="argG"
                     /locus_tag="WHQ94_000330"
     CDS             complement(10031..11374)
                     /gene="argG"
                     /locus_tag="WHQ94_000330"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ2127677.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11701..11841
                     /locus_tag="WHQ94_000331"
     CDS             11701..11841
                     /locus_tag="WHQ94_000331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127678.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11838..12638
                     /locus_tag="WHQ94_000332"
     CDS             11838..12638
                     /locus_tag="WHQ94_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ2127679.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12678..13664
                     /locus_tag="WHQ94_000333"
     CDS             12678..13664
                     /locus_tag="WHQ94_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ2127680.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14321..14583
                     /locus_tag="WHQ94_000334"
                     /pseudo
     CDS             14321..14583
                     /locus_tag="WHQ94_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14621..14995)
                     /locus_tag="WHQ94_000335"
                     /pseudo
     CDS             complement(14621..14995)
                     /locus_tag="WHQ94_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15168..15524)
                     /locus_tag="WHQ94_000336"
     CDS             complement(15168..15524)
                     /locus_tag="WHQ94_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ2127681.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15667..16011)
                     /locus_tag="WHQ94_000337"
     CDS             complement(15667..16011)
                     /locus_tag="WHQ94_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127682.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16170..16570)
                     /locus_tag="WHQ94_000338"
                     /pseudo
     CDS             complement(16170..16570)
                     /locus_tag="WHQ94_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16574..17314)
                     /locus_tag="WHQ94_000339"
                     /pseudo
     CDS             complement(16574..17314)
                     /locus_tag="WHQ94_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17384..17701)
                     /locus_tag="WHQ94_000340"
     CDS             complement(17384..17701)
                     /locus_tag="WHQ94_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127683.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17698..18300)
                     /locus_tag="WHQ94_000341"
                     /pseudo
     CDS             complement(17698..18300)
                     /locus_tag="WHQ94_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18726..19070)
                     /locus_tag="WHQ94_000342"
     CDS             complement(18726..19070)
                     /locus_tag="WHQ94_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127684.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(19108..19452)
                     /locus_tag="WHQ94_000343"
     CDS             complement(19108..19452)
                     /locus_tag="WHQ94_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127685.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(19455..19643)
                     /locus_tag="WHQ94_000344"
     CDS             complement(19455..19643)
                     /locus_tag="WHQ94_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ2127686.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(19713..20057)
                     /locus_tag="WHQ94_000345"
                     /pseudo
     CDS             complement(19713..20057)
                     /locus_tag="WHQ94_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(20141..20443)
                     /locus_tag="WHQ94_000346"
     CDS             complement(20141..20443)
                     /locus_tag="WHQ94_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127687.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(20466..20735)
                     /locus_tag="WHQ94_000347"
     CDS             complement(20466..20735)
                     /locus_tag="WHQ94_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127688.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(20756..20962)
                     /locus_tag="WHQ94_000348"
     CDS             complement(20756..20962)
                     /locus_tag="WHQ94_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127689.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(20928..21677)
                     /gene="mafB"
                     /locus_tag="WHQ94_000349"
                     /pseudo
     CDS             complement(20928..21677)
                     /gene="mafB"
                     /locus_tag="WHQ94_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(21783..22154)
                     /locus_tag="WHQ94_000350"
     CDS             complement(21783..22154)
                     /locus_tag="WHQ94_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ2127690.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(22151..22402)
                     /locus_tag="WHQ94_000351"
     CDS             complement(22151..22402)
                     /locus_tag="WHQ94_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127691.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(22534..22740)
                     /locus_tag="WHQ94_000352"
     CDS             complement(22534..22740)
                     /locus_tag="WHQ94_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127692.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(22750..>23226)
                     /gene="mafB"
                     /locus_tag="WHQ94_000353"
     CDS             complement(22750..>23226)
                     /gene="mafB"
                     /locus_tag="WHQ94_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2127693.1"
                     /translation="ATTVWGGKKVELNPTKWDWVKNTGYKKPAARHMQTVDGEMAGGN
                     RPPKSITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFAR
                     HIENIVSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
BASE COUNT         5585 a         5385 c         5705 g         6551 t
ORIGIN      
        1 cttagctcaa agagaacgat tctctaaggt gctcaagcac cgagtgaatc ggttccgtac
       61 tatttgtact gtctgcggct tcgtcgcctt atcctgattt ttgttaattc actatatcga
      121 ttctgttccc ttgcaggcgg acggcggcgg cggacggtgg aaaagcaggt tttcaaatgt
      181 ccggtttggc ggcgcgccgt ctgaaaaccg cttattttcc tttgctgttt ccggcggagt
      241 cttttttgtc cgaaccggag aagcggtcga aacgtatggt gtatgtcagc tcgccgcccg
      301 acgaacggct accgatacgg gcaaccgcct gtatggcgcg ggtcagccgg taaatcagtt
      361 tgacggactg ttccgcgctg gagatgctgt attcgtagcc gatgtagagt ttgccggtca
      421 gttgtttgcc gacggtcagc acctgttcgg cggggttgag ttcgccggtt tgcgcgttgc
      481 ggctgcgctt gctggtaaag cccaaatcat ccaccagccc gatgcggtcg ttgatttgcc
      541 cggcaagcag cgcgccagcg gctgcggaca gggcggcatt gtcgccgctg ctgccactgc
      601 cggcgcggtt gaggatgagc caggagagct tgtctttttc actcatcggt tcgtttgccg
      661 tcagcgtaat gcgcggactg ttgaggctgc ccaatatttc cacgcccgca ccgacggggg
      721 aaaggcggcg ttcggcgcgg atgttgaggt tggggtcgtt gagcgggccg acaaaggaga
      781 ccgtgccttt ggtaatgtcc aaatcctgcc cgtatgcctt ataacgccct ttgatgacgc
      841 ggaccgtgcc cacgccccgc acgcttccgc ccgattgggc ggtcagggtc agtttgccgc
      901 ctatggtaac gtccgcgccg tagccggcga agcggatgcc gtcattgagg tctaaagtca
      961 ggttcatatt gacggggagc ggtgccgccg cctctttttt gacttcgcct aatacgacga
     1021 catcgtcgcc gacggacggc atcgaggatt tttgcgaacc gaacagcccc tgatccgttt
     1081 taatcatccc ggtaacggat atgccttttt gcggcgaata gcgcaggcgg gtgttgccgg
     1141 aaaccgtcag gcggcggttg gggcgggaca ggatgcggta tttgtcgaac accgcgccga
     1201 tatcgacatc gggtccgctg ttttctatac cgaccgtacc ggagagttcc gccgtccctt
     1261 cgtgccggaa tttcaggctg tcgattaccc atttcctgcc cgcgatatgc gaacgcagcg
     1321 agccgttgtc caagatgatg ccttgggttt ggttgcggta atagagtttg tcgccgttga
     1381 tgctgccgcc caagtgcgga tcggcgatgc tgccgccgag ggttacggcg gcattcaggc
     1441 tgcctttgac ggtttgtccg acgggcagga agttgcggaa tacttcggca tcggcaacgg
     1501 tcaggttgag tttcccgccc aggggcgagg tactgaaaga attactttgc ccgacggtga
     1561 tgttgccgtt gattttcccg tagttgctgc tgacgttgac ggcggcattg acggacggag
     1621 agccgacccg tccgccgatt tgcgcggcgg cattcaggct gccggtaatg ttttgcgcgg
     1681 cggcgggcag aaagggcttc aatgtgccca agtcgggaag ggaggcggta atcctgccgc
     1741 cgagcggtgc atttgccata ttgccgccga aggcgttgcc gatgtccaaa tcggcgttaa
     1801 tccgcccgaa acgcgcgccg ccgtcaagca ggattccgat acggtcgttt tgaaagcgcg
     1861 ttttcaggga aaatgcgttc aaacccaaag cctgcccgcc gggcaatacg gcatcgccgc
     1921 tttgccggct gatattgagg tagccgcgcg cgttgcgccc gtaggcaaca tcccagtcgc
     1981 cgtttaaaac cagattgtgt tcgaagggcg gtttgaagaa attgtgcaac tcggcgatat
     2041 gcagaccgtg tgcgccgcct tttgccgata tgccggtttt tttatcccaa gaaaagtgtt
     2101 gcaggttgag gctgccgccc attgcctgcc aatttgccgc acttgccgcc acgcgttccg
     2161 caccggcttc gagcgtcata cggttttgca gcttgaggtt gaatgcgccg ccgatgtcga
     2221 ggatgccgat gctgcctttc catcgggtaa gttccctgtt gatgccgcct gaagcgtcca
     2281 aatcgaattt gaacggtttg ccatccagcg tcatggcggc gtgtgtgcgg atgcggtgct
     2341 gcacgcccgt gccgtccagc atcaggtcgg cggtatcgac aaccgccgct ccgcccgaca
     2401 gcgaaaggcg gctgcctttg atgtcggcgc gtatcgggcg gcttgtgtcg ggcgaacctt
     2461 tgagcgtgaa atcgagcgaa cggatgtctg ccgccttgcc gatgtgcagg ttgcgcgccg
     2521 cgccggaaag gtcggtttca aaggttcgga tgccgccgtc caaatcaccg gaaaggtgtc
     2581 cgcgtacatt taaagacccc gcgagtccga aaccgaaacg ggataaatcg ggtgcggtga
     2641 tattgaggtt aagccggtcg ccttttttgc cgaagccgcc gtctgtttta ataatgttcc
     2701 gccccagccg caaatcgacg gcggcacgcg gaaggtggcg ggactcgtaa acaatgtcgg
     2761 cactgccggc aatcggtacg ccgttgaacg tgccgggtaa aaaccgcatt ttgcctgtga
     2821 atttctcttt tgccagttcg ccggcaaggt ttattgagcc gttgatattg cctgccggaa
     2881 gttgcggatc gatgcgcgaa gggtcgaatg cgcgggaacg gatgtccagc ttgagcaggc
     2941 ggtctttaaa cagctcgaga tagccttgcg cggtcaggct gccttgcccg gcggcgatgt
     3001 tgacggtgtc gagcaccagt ttccgctgtc cgtttgcggg gtcgcttgca atggcgaggc
     3061 tgccgtccgt gcgcgctgtg ccgatgccga gttgccaaga gattttgggc gaggcggtcg
     3121 tgccaccgat gccgatgctg ccgtccaacc tgcctttgaa cgcggtttgc agcacgtctt
     3181 ccgcgccgac ggagttgatg cctatattta aatcgaggat gtctttttcg gtgtcgattt
     3241 tgcccgacag cctgatgccg ccccgtccga gcagggcggc ggacgtattg ccgatatgca
     3301 ccgtgccgtc ctgccggatg acaaagttgc ctaaaacctg acggacgggg atgccgttgc
     3361 ggtcggcaaa accggcttta gtgttttcca aatcgagcga gccttccagc gcgatgccgt
     3421 ctgaaaacga cgggatggcg gtcaggtcga aattcagccc ggcatcgggc agggaaggca
     3481 cgaaggcgga cggattgatg ttgaaccctt tgaccagtac ttcttccaat gttttatcca
     3541 atgattcggc aaacgggtgg atgacggatt ttcccgagag gcggatattg ccgccgtcga
     3601 tcgccagttc ggcgcgcaca tccttcaggc tgccgctcag ccgagccgta ctgtgtatgg
     3661 ttttgccttc gagtccgcct ttggtgtaaa tggcggtatc gagggcaaac ggttttttca
     3721 agccgaccga ggctgacccc gacgaattgc tccacggcgt gtcggcagcc ttcaggtcga
     3781 ggcggtgtcc tttgatgtca taacggtatg aagcatccag ccgttcgaga tagacggttg
     3841 gtttgtcaaa ggctttgcct gcgctgattt tgcccgtctc gaagcggtcg agatagacgg
     3901 cggcaggcag gtctatgctg tcgggaaggc tgagcggagg gcgttcttct ttaggcggag
     3961 tcggtttggt aacgatggcg atgtcgccgg cggaaatttc ggtgatgtgc aggctgcggc
     4021 gcatcagttc ggacggtttc cacgcgaagc ggaagcggct gattttaagg tctgccccct
     4081 cggtttctat cgaccagttg tcgccgtcga agccgtcgag cagcgtgcct ttgaggtttt
     4141 gggaggaaat gtttacgccg aaccaagacg ggatttggta cagcccgaaa cgcaaaccgg
     4201 attccgtacc ggcgagccag ctgaggaaac atactgccaa aatcaggaca gacagcagtg
     4261 ccgccgacag cttcagcagc gggcggcgtt tttttgccgg cggggtgggg cggtgttcag
     4321 acggcatttt gcgcgtgccg ttttcggtcg gatcggtatc tgtcggtgcg gtatcggtca
     4381 taatgtctgt ttcaaaatgg tttgctgtaa tgccgtctga agcggccgta tcggtttaga
     4441 agcgtgttcc caagctgatg tgccagcgga ttttcttatc gctgtgcccg taggcgatgt
     4501 cgaaggaaaa cggcgcaagc gggctgaacc agcgcacgcc cagtcccgaa ccgtgtttca
     4561 gcttcatacg tttgaaattg gcggcggcat cgcccatatc gtggaatacc gcgccggaaa
     4621 gggtgcgcgt aaacggcagt tggtattcca ggctgcccac caagagggcg cgttcgggca
     4681 ggaccgatcc gttcgggccg gcaagtccga tgctgtcgag ttcgtaaccg cgcacggaag
     4741 acgcgccacc gctgcggaac atcagccccg aggggacatc ggcattgtcg cgggcaaggg
     4801 tgtaacccgc ttgtccgcgt atgatgaacg tgccgagttt cttgttttcg ggcgtgaaga
     4861 aataacctgc gcgggcagag gtgcggatca gcgcggtgga ggacaggaat gtgcccaaag
     4921 tcgtaccgat tttgccgtcg aggtaatggc cgttttcggg atgcagcacg ttgttgagca
     4981 gctggcgttt ccaagaggcg gtcagcatcg tggcgtggct gttgcccaaa tcgatatccg
     5041 agccgggaat tttccggcct tctgcgagaa actctgcccc cagcctggca tcgatgcccg
     5101 cgcggtcgcg cacataccag atgccgccgg agaaggcgcg tttttcgagg ttttgggtgg
     5161 tcgaacggtt gtaggaaacg ttgcttgtcc agtagttgcc ccgatagttg cgcggctggc
     5221 tgatgccggc ggcaagcgtg gtttcgtatt tgtccatatc ccagacgacc gagccgatat
     5281 agcctttgtt gaagaggttg taatagtcgt aggcgatttt gccgcccaaa ccgtattccg
     5341 aatcgaggcg gatgccggtt tcgagcttgt ggcgtttgac ctcggttacg ctgactttga
     5401 cggggacgcg gtcgccttgg agacggtcga agtcggcttg tacggacgcg ccggaataat
     5461 gcccgttttg ttcgagtgcc tgttggaagt cgagcagcag gtcgaggtcg tagggcgtgc
     5521 ccggttggaa gcgcgccagg ccggagacga tttgttcggg gtaacgctgt gtgccggtga
     5581 tttcaaagtc gccgaaggcg atggggcggc cgctgtccac gacgacgttc aaatcgacgg
     5641 tggcggtatc ggggttgacg gccgcccggg tgttgccgag tttggcaagc gggtagcctt
     5701 tgcgcgttac cgcgccgagg acggaagttt tgctgttttc ccaactgtcc tgatcgaaat
     5761 cgctgcctac cggctgctgc cagttttcca gcgcgttgcg gtagtattcg gcgaggttgc
     5821 cgtctgaaag gatgtcgccg aggatggcga cgccgacgtt ggcgattttg gtgcgcgggc
     5881 ccggtgtgat gtgcaccgta taagctccgt ctttttccgt cagactgact ttgctgctga
     5941 aatagccttt gctgcggagc atcgttttaa cgttgtccgg tgcttcttcg gcgaggaagc
     6001 ctgtctgttc cttatccaaa acctcttcct gctgctgcgt gatgagcggc aggtgttctt
     6061 cgaccatatc tttgatttca ctgtcctgcg tgtcgatgcg gacggggaat ttgggtttta
     6121 atttaactga ttcggtgtca gggcttttgt ttttgaacaa tgcgaaaccc gccgccttgt
     6181 tttcggaaag gtcggcggca ggcgcgtatg cgtgcggaaa gaaaaataaa gccggcagga
     6241 gcagggcggt cggtttgatc atcatagtac gggtgtcgtg catcggtgtg gcggatttcg
     6301 attctaacac tgttcggaca gggtttcgaa cggccgtttt tgtgttgttc ggacggggcg
     6361 ggacgcttca gacggcatac tgccgccggt atttcggagg ttgcggcccg ccggaaatgt
     6421 atctgcttgt tttaaggtat tttgtttcca atctgcatat ggaaaacagg ctgcgtttcg
     6481 gaatagtgcc ggaaaataaa cgggattaaa taaataaatt tattttcgtt caataaatta
     6541 aaccgtcgcg ccgaatgata aagcaaagcc tgtccggcac tgcatagggc aaatcaacgc
     6601 accttaaaac acagccgaaa aataaaaaaa cacagagaat gagttttctt ttaaaataca
     6661 acatttttta acatatcaca acataaggaa gaattatggc gttcggcaaa tccttatttc
     6721 atgcgatcgg ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg ttgggtatcg
     6781 taatcggttc ggtttccccg caactgggct tggcggcagg cttgttcggc agcctgtttg
     6841 tcggcgcgct caaagcggtc gcgccggttt tggtatttat cttggtggcg gccacaatcg
     6901 cacagcacca aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt tacctcatcg
     6961 gcacgttttc cgcagccctg accgccgtca tcgccggtat ggttttcccg acgcacattg
     7021 ttttggcggg cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg gaagtgttga
     7081 aatcgctgct gatgaacctg gtcgccaacc cgattaacgc gattgccaat gccaactata
     7141 tcggcattct ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac ggttcggacg
     7201 ttacgcggca ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg aaatggatta
     7261 tccgttttgc ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg gagacgggtt
     7321 tcggcgcgct ggcgggttac gcgaagctgc ttgcggtgct gttgggctgt atggcgttta
     7381 tcgcgctggc ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac ccttatccgc
     7441 tggtgtttac ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt tcttccgccg
     7501 ccaatatccc cgtgaatatg gctttggcga aaaagctggg gctgcacgaa gacacttatt
     7561 cgatttccat cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt acgattaccg
     7621 ttttggctat ggcggcggcg cacactcaag gcatacaggt tgattttgcc accgccttgc
     7681 tgttgagcct ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggca ggcggttcgc
     7741 tgctgctgat tccgctggcg tgcagcctgt tcggcatcag caacgatgtc gctatgcagg
     7801 tggtcgccgt cggctttatc atcggcgtga ttcaggattc ggcggaaacg gcgttgaatt
     7861 cttcaaccga tgttttgttt accgctgccg ccgatttggg ccgacagagg aaccgggcgg
     7921 aataaaccaa gctatgccgt ctgaagcccg tttggcgttc agacggcata tttttacaat
     7981 tttatgggca tatggcggaa acaataccgg ctgccttgaa tatgaaggag atgatgatgt
     8041 ttaaacgcag cgtaatcgca atggcttgta tttttgccct ttcagcctgc gggggcggcg
     8101 gtggcggatc gcccgatgtc aagtcggcgg acacgctgtc aaaacctgcc gcccctgttg
     8161 tttctgaaaa agagacagag gtaaaagaag atgcgccaca ggcaggttct caaggacagg
     8221 gcgcgccatc cgcacaaggc agtcaagata tggcggcggt ttcggaagaa aatacaggca
     8281 atggcggtgc ggcaacagcg gataatccca aaaatgaaga cgagggggcg caagatgata
     8341 tgccgcaaaa agccgccggt acagacagtt cgacaccgaa tcacaccccg gctccgaata
     8401 tgccggccgg aaatatggaa aaccaaccgg atatggcaaa tacggcggac agaatgcagg
     8461 gggacgatcc gtcggcaggg gaaaatgccg gcaatacggc tgcccaaggt gcaaatcaag
     8521 ccgaaaacaa tcaagccgcc ggttcttcag attccacccc tgcgtcaaac cctgccccca
     8581 cgaatggcgg cggcgatttt ggaaggacga atgtggctaa tggcgttttg attgacggac
     8641 cgtcgcaaaa tataacgttg acccactgta aaggcgattc ttgtagtggc gataatttat
     8701 tggatgaaga agcaccgtca aaatcagaat ttgaaaaatt aagtgatgca gacaaaataa
     8761 ataactacaa gaaagatggg aagaataata ataaatttgt cggtttggtt gctgacaggg
     8821 taaaaaagga tggaactaac aaatatatca tcttttatac ggacaacccc cccactcgtt
     8881 ctgcacggtc gagacggtcg aggaggtcgc ttcctgccga gatgccgcta atccccgtca
     8941 atcaggcgga tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca
     9001 atatcttcgc gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg
     9061 gaggatcgta tgccctccgt gtgcaaggcg aaccggcaaa aggcgaaatg cttgcgggag
     9121 cggccgtgta caacggcgaa gtgctgcatt tccatacgga aaacggccgt ccgtccccgt
     9181 tcagaggcag gtttgccgca aaagtcgatt tcgtcagcaa atctgtggac ggcattatcg
     9241 acagcggcga tgatttgcat atgggtacgc aaaaattcaa agccgttatc gatggaaacg
     9301 gctttaaggg gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggtc
     9361 cggccggcga ggaagtggcg gggaaataca gctatcgccc gacagatgcg gaaaagggcg
     9421 gattcggcgt gtttgccggc aaaaaagggc aggattgatg ttgatgccgt ctgaagcggg
     9481 ggcgttcaga cggcattgtg atgtttgggg tggggtccgg ttcaaatccg catcagttga
     9541 taaacccgtc gtctttcatt ttcttttcgg aaaaatactg accgcatacc agtaaggcgg
     9601 cgaggatgat gacaaacagc cccgccatag tcagtgtgat tttgaagaac aattccgctt
     9661 cgagaaaaga aaaccagagt tgcacgatca gcagcagcac ccagccggca aacagtccga
     9721 gacaggtata aacgccgagc ttaaacacga tcccgctcca aaatgacgac gtaggcctcg
     9781 cgtttgaaaa acagcagcat acggcgcaaa tctttttcca tcgttacgac ccgccagcct
     9841 tcgggggttt gtttgttgag gaacgcgctg aaattgacgg ggttgacctt tgccgtgccg
     9901 aaaaacaggc tggacaacag gctttcctga taaatgacga ctttgtattc tttcatagga
     9961 ttccttttaa cataagggat catcttttca gacgacccct taagttgcta atgatgcaaa
    10021 acagcaaacc ttattgctta ttgcccaact gcggcaatac cgaaccttcg cccagcgaga
    10081 gcaaaccgct ttgcgaataa atacccagtt tggcgcgggt gtcggtgatg tcgaggttgc
    10141 gcatcgtgag ctgtccgatg cggtcgagcg gtgtgaacgc tgcatcttcg actttttcca
    10201 tactcaggcg ttcgggttgg taggtcaggt tgggcgattc ggtgttcaga atcgagtagt
    10261 cgttgccgcg ccgcagttcg agggtgactt cgccggtgat ggctttggcg acccagcgtt
    10321 gggcggtttc gcgcaacatc aaggcttggc tgtcgaacca gcggccttgg tagagcaaac
    10381 gtccgaggcg caggccgttg atgcggtatt gttcgatggt gtcttcgttg tggataccgg
    10441 tcaccaaacg ctcgtaggcg atgtggaaca acgccattcc cggggcttcg tagatgccgc
    10501 gcgatttggc ttcgatgatg cggttttcga tttggtcgct catacccaga ccgtggcggc
    10561 cgccgatgcg gttggcttcg aggaagagtt cgacgggatc agcgtattct ttgccgttca
    10621 gtgcaaccgg cacgccttct tcaaagcgca cgctgacttc ttcgggtttg atttcgacgt
    10681 tttcgtccca gaaggcaacg cccataatag gtttgacgat tttgatgccc gagttcaaaa
    10741 attccaaatc tttggcttcg tgggtcgcgc cgagcatatt ggaatcggtg gaataggctt
    10801 tttccaccga cattttgtag ttgaaaccgt tggcaatcaa aaattcgctc atttcatgac
    10861 ggccgccgag ttcgtcgata aattgctggt cgagccaggg tttgtagatt ttcagcgcgg
    10921 gattggtgag caaaccgtag cggtagaaac gctcgatgtc gttgcctttg taggtgctgc
    10981 cgtcgcccca aatattcaca tcgtcttctt tcatggcgga aacaagcata gtgccggtta
    11041 cggcgcgacc cagaggcgtg gtgttgaagt aggcgatgcc gccggtggaa acgtggaacg
    11101 cgccgcattg gatggcggcg atgccttcgt gtgccagctg tgtgcggcag tcaatcaggc
    11161 gggcgttttc cgcaccgtat tccattgctt ttttgggaat ggcattgtag tcgtcttcgt
    11221 cgggctggcc gaggttggca gtgtaggcat aaggtagcgc gcctttgagt ttcatccaca
    11281 acagcgcggc agaggtatca agaccgccgg agaaggcgat gccgactttc tgaccgacgg
    11341 ggaggttttg taaaatagta tggttttggc tcataagggt atccttcgga cttggaaaaa
    11401 ggagttcgtc gtgtttcctt ttcgattgaa accccgccac ttggacatct gtccttcggg
    11461 gcggtagaat cagactttat ttgggagggg cgtaatccct tccaaatcag gacggcacat
    11521 agggcggtgc tttatgtgtc gtcccgtgtg ttgaaacatt atcttataaa tataacccat
    11581 tgtcagccgt taaggctgtc tgaaaagaag ctttgcccgt ttcgctgaaa atcttttcag
    11641 acggtcttta cgccatcctc aacctgactc cctcccctgc gggggagggt tggggagagg
    11701 gtgcagacag cagcccttaa gcaaacgctg cctgaaaaac gcaagctcga ccctctccct
    11761 aaccctctcc catgggagag ggaacaaatc acagcaccat cgacattttc agacggcatc
    11821 ggtaaagaaa cccatctatg aacgcgttca acctcaccat catcggcgac gaaatcttac
    11881 acggcagccg ccaagacaag catttcgcct ttttcaaatc cctgctcgaa tccaaagggc
    11941 tgaagctcaa tcaggtgcaa tatctgcccg acgaacccga tttgctggtc aggcaactgc
    12001 gccgcagctt ttcagacggc ataccgacgt ttgttaccgg cggcatcggc gccacgcccg
    12061 acgaccacac gcgccaagcc gcagcggctg ctttggattt gcctgtcgtc cgccatcctg
    12121 aagccgccaa gtttatcgaa ggcgtaaccc ggaaacgcgg tgagccgctc gattcgcccg
    12181 aacacgccca acgcctgaag atggcggatt ttccccaagg cgcggaattg gtgcctaatc
    12241 cgtttaataa tatcgccgga ttttccattc gcgagcatta tttcttccct ggttttcctg
    12301 tgatggcgca cccgatggcg gaatgggttt tggatactta ttacgtcggc cgcttcaacc
    12361 aaaccaaacg cggcagccgc agcgtgtatg tgtttgagca gcccgaatcg cgcattacgc
    12421 cgcttatgga gcatatcgag caaacttatc ccggtgtgcg ttcgtacagc ctgcccagcg
    12481 tcggttggac gcattcagac ggcacgcagg tcaaaccgca cattgagttc ggcatcaaag
    12541 ccgaaggaga agccgtcaac cttttggacg cggcgtgggc ggaagtgttg cacagcttgg
    12601 acggattggg agcagagctg aaaaatcggg taaactgaaa accgtttgcc caaatcggac
    12661 gatatttaag gaaaaccatg caataccgaa tcagaagaga aaacgaagca cccgaagcaa
    12721 aaaatacggg ggaaacctta tgggagcgcg acatcatgcg cgaagtgctg ctgtccgcct
    12781 atcaggacag gcgcagggag cggatgtgga aaaacatctg gcgtgccgtc agcaccctga
    12841 ttctggttgc cctgattgca ggcattttcc gaaaagacga agccgcattg cagttggcgg
    12901 gcaacacgcc gcataccgca gtcgtcaacc tgtacggcga aatcggcaac ggtgtagaag
    12961 accaggtcaa aaaactcaaa gacggtatgg aggccgccta caaaaacccg caggcaaaag
    13021 ccatcgtcat ccgcgccaac agccccggcg gttcgcccgt cgtctccaac accgcttttg
    13081 aagaaatacg ccgtctgaag gcgcagcacc ccggcattcc cgtttatctc gtggcggaag
    13141 atatgtgcgc gtccggctgc tactacatcg cggcggcggc ggacaaaatc tatgccgacc
    13201 cgtccagcat cgtcggcagc atcggcgtta tcggcagcag tttcgacgcg accggcctga
    13261 tggaaaaaat cggcgtgaaa cgcagggtta aaattgcagg cagcaacaaa ggcatgggcg
    13321 atccgttttc gcccgaaacg cccgaacagt cgaaaatctg ggaagaaatg ctgaccggca
    13381 tacacggcga attcatcaaa gccgtcaaaa ccggacgggg cggccgcctc aaattccgac
    13441 aatatcccga cgtgttcagc ggtcgcgtgt acacaggcgc ggatgcgctg aaagtcgggc
    13501 tggtggacgg actcggcaat atttacagcg tggcgcgaga cgtggtcaaa gcccccgatg
    13561 tggtggatta cactccgaag gacgatttcg gcagaatctt gggcagacgc ttcggcgcag
    13621 aactcaaagc aagcgtgcgc gaagccctgc aggccgtccg gtagcgttgt ttacagtatt
    13681 tctaagggaa taccaaaata aaaatataca caaaaacact cacctcccca gtatgctgcc
    13741 actaagtcag ccgcgtcact atttaccgtg cacttaaagc cgtaaaaggc aggttgtccc
    13801 cgggaggttt tgtgcatcct gataccgatc ggttgttgtt gctcaaagga cagaaggccg
    13861 ctgataaacg agattacctg tttgtcgcta ttgacgattt ttatactctg ccattttgcc
    13921 agacaaaacc gcagacagtg ctgccaagtt tctgaccgaa catctggccg acccctgctt
    13981 gtacctgatt gagtacgttt actctgacaa tgataggtaa tataaagagc cgtccaacat
    14041 gctttcggtg cagcttgtta taataatggg attggttgga ggcttgcccg atttgcttgt
    14101 ccgcagacca acggtaaggc ggagcgggtt atccgtacct tgatggagat gtggcatgag
    14161 gaacagtcgt ttgacagtcc ggaacatcgg caaaaggagt tgtgctgctt tattaattta
    14221 ggggctaccc tagatcacta gggaaattca aattaagtta gaattatccc tatgagagaa
    14281 agtcgtctaa gccggtataa acaaaataaa ctcattttgt tatcgtggct atgatgtatt
    14341 ggatgcgggc gaatttagcc atttccgtat caatcacagc acacattttg ccgaacgaca
    14401 aaaccatatt aatggaactg agaacttttg gaatcaagca aaacgtcatt tacgcaagtt
    14461 taacggcatt cccaaagcgc attttgagct gtatttaaag gagcgcgaat ggcgttttaa
    14521 caacagtgaa ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc gagtttatcc
    14581 tagttgtcca gggcagcctc atctctttat agatagctgc ttaccaagta tccttaattg
    14641 ctaattcaag ccaatcttca aaactattaa aaaattcatg gttttctata ttatctaaat
    14701 taatgacaat aaccctagaa tttcctgtta catcattacc atcaaaataa gcttcccatt
    14761 ctccattact agcgaatggg ataagattta aatcaggcag gatagaattt ctaaatatat
    14821 aagctacttc tgccccttct gttccataat tctcaaacca ccagtgaaaa ttatgtggct
    14881 gaatataagg tattaaagat gtatcgttag agatacgaac atacttttca ggataacaaa
    14941 acctttcagg aagcatatta ctaggatata aattaaattc attttaaata atcatttcgt
    15001 taccttagga tcataaatac taccattagg aaactgggta gaccaatttg gtggtgctag
    15061 ttcattttca atttgttttt taattctaaa ttgaatagct tctccccatg tttgcggcat
    15121 ctaatgctgc ttgctctgca tcaatattac taaaaattgg attagtctca aaaatgaaaa
    15181 attactgttt tttcattttt tatagcatat tcaagtacat gaataatatt tttcactaag
    15241 ggctctgtag aaaaatctga attttgtaat ttttctttta cagccaaaat actttcctta
    15301 tctattagcc aatccccctc acttggtcca aataaccaat tatcggtaat ttcagaaaca
    15361 agtctccata atccacgatt ttgatattca ataatttgac tttccgtaat ttctacaact
    15421 gtttcagatt gaatgttttt ataagcccaa tcaatcattt cagtatattc actaattgaa
    15481 acattgtctt ttaaacagaa aattaacagg ttgtgttcat tcatttgtct aagttcttaa
    15541 tatgagtttg ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta aaggattaac
    15601 cgcaccttgg atgatgccgc gaatatcatc catacggtta ccccaccaaa ttttattggt
    15661 gtatcgttaa tcatttattt caaaaaagtt atttattatc atattatcag ggagcaggtc
    15721 gttaaaaaaa accccctcaa attccattaa attagtattt atccagcaca taaacgaacc
    15781 aaagcgttct ttccctgagt gtaaatattt aaaattaaac tcaaaataag tttttttgct
    15841 tagaaaattt aatggttttt ctatagtgaa aaaaaagccc ttactcgttt tagttcgaga
    15901 tactatttta aataaatttt tattttcaat aaaataagaa tattcattta ctaagttgtt
    15961 atgataaaaa agtaatttta ttaaaatgtt ttcataattt tctaggttca tttctagctc
    16021 aatttaaaat gatttcattg ttgccgtacc aatgcgaaat ttaccatcaa caaaatttcc
    16081 cttcactgat acaggtacgc catcaacttg ggtagtttta ttttcaggct gcctgaaaga
    16141 aagataggca acttattact catcttacgc tagccctcaa taattgaata cgttgcacta
    16201 gaaccaatta tttctatgaa tgtttgctca aacataaaaa tatagtattt aaagaattct
    16261 ctattcaaag gaaaataact ttctttcaat atccattccg cataacactc atcaagaagt
    16321 tcaaaaaaga ttgaccacca ttttcatgaa gcaaagctct atctgcatct tccgaaatta
    16381 aatccataaa agtatctata aaatacgatt catcaacaac cctaaaatga ataacatctt
    16441 caataaaaat ttttatggtt ttatccgtat ttctataacc aactgctata acttctactt
    16501 tatcagagtt tattgctgca ttatcaaaat agaaatagcc agttgggaaa tctaagcttt
    16561 ttaatttcat aatttatcgt gtatttccat atctgatttt tatttttcgg ttattactaa
    16621 ttgttatttc aagtgttggt tctccaccac tactatcatt acgagcatta accgcacgcc
    16681 tatcaggtaa attgcccgtt atcgttccat taggacgtgt ttgtacattt cgaacattca
    16741 gactattaaa atcatttagt gcgtcattgt aatcccccac tttatcatac tgaacagcgg
    16801 gaccttttgt tttactcctt tgagtagtat tgctcaaaat gtctttgatt tgtaaatcac
    16861 tgcgcacctt accggtttct atgcgagctg ccacactact cgggctatag gggtcgctat
    16921 caatcgcagg attaccaccg ctggtttttt gtccgctagc ctgtttccca acctgctgta
    16981 ctgtatcgcc aatttcatct accgtaccta acgccttagt ctgcatatgg cgaacagcag
    17041 gttttttata gccggtattt ttaacccaat cccatttagt cgggttaagt tctacttttt
    17101 tacctctcca aaccgtacct gcggcctctg ctacggaaag ggcagtttgg gctgttgcag
    17161 ttatattcgg atgggcatca gcccattgtc tggcagagtt gatactgtct tttaccgcaa
    17221 aagcactgtc ttgtaatgcg gttgcggccg caagttgtgc ttcgggactt aaatttccta
    17281 aattattaat accttgtaga gtctgctgcg cagagatgta gcaactgtat ttttaccccg
    17341 tcgggtaaaa atacagttgc tacggctgct gggaaagcag ttactatttt cccagtggct
    17401 caaaattaat tgtttcagca gatattttta taataatccc tacatcaata ataatttcac
    17461 tataaaattg gtatttatat agctctattt cgggtataaa caagggaaac aagctatctt
    17521 ccttatcctc ataatctacg aaattatttg tatctaattt aaaattcaaa atattactgc
    17581 atttgatata tccgaaataa ttaccttcaa ttgtatctgt aaaagataat gtaagggtat
    17641 ttccattatc ggaaaagatt atttcataaa ggcttacatt gtgaaaaata ttttttttca
    17701 tttgacaaaa tatccagaat gatgacgatt atttggcaat ggattgcctt cataatcttg
    17761 tcgttgcgga tattttccct gtacaccatc ggttcgcggc actctttggg ttcttataac
    17821 ctctccattt ggacgaatat ctattttcac gccatcagga cctttccatg tttcatattg
    17881 agcctcatct gaaccagtac gtctaaaacc ttgttcttgc aatgcttgac gtaattcatc
    17941 tcgcgtagtt ggtctgatag atttaacaac tggcttgttt cctcctgcca tctcaccgtc
    18001 caacgtctgc ataggtcggg cagcaggttt tttatagccg gtatttttaa cccaatccca
    18061 tttggtcggg ttaaggttta cttttttacc gccccaaacc gtacctgcgg cctctgctat
    18121 ggcaagagca gtttgggctg ttgcagttat attcggatgg gcatcagccc attgtctggc
    18181 ggaattgata ccgtctttta ccgcaaaagt actgtcctat aatgcgcttg cggcagcaag
    18241 ttgtgctttc ggacttaaat ttcctaaatc attaatacct tgtaaagttt gctgcgcaga
    18301 gatgtaggtc ggatatttgt atccgacaaa aatatttgac gcatctatcg tttccgaaat
    18361 gccgctgttg gaaaatgtcg gattcgagaa tccgacctac ggctatttcg tttaagtcat
    18421 aaatagccat tcatgttcct tctgaatttg cttttgctat ccttagaacc aatcatataa
    18481 acattatcat cattaattaa tgttctaagt ttatgaagcg gtcaaatctg tcaaactacc
    18541 agagatgaag catatcctcc tggaagtaat tctgttcgtg ctgtttccat atcttctttg
    18601 ccatcatcag ttacttctct gtctaaataa aatcttacga taagtttttg tctaatttca
    18661 tcaaattctg ccgtaattaa tcttatattg atatgaatac acggttcata cctgctatta
    18721 gccagtcagg tgtagttgga tggggattca tttttaataa tatcttcaag caagtgaaat
    18781 gtaagaaaag cgtagatact tgaaggccgg ctatctgatt ggcaaaaaga aatttcggag
    18841 ataaagaatt tccgtttata tttttcataa attaaatcaa caccttgttt tacataaaaa
    18901 ctaacttcct caggaaggat atttataaca ttctctcctt ttacttttat agttaatggt
    18961 ttgattttta tgttttcatg aatatctgat aggattattt ctaaaaaatt atttttattt
    19021 ccagtcattc tggctatttt atgtatattt ccattgtgtt cgattatcat tttatttttt
    19081 ccaaggattt ccttctatat tgatagttta tctaaagatt tcatttaaaa tatcctgact
    19141 aatttcttgt ttatataatt ctacaatttt ctgtttctgt gtatttgtat aaaatttttc
    19201 gttggtatct gcttgatatt ttaattgtct caatatttcc atgatttcat attctgattt
    19261 ttcatcatat tcttcaatca tgtaatcaac aaattgtttt agaatatcaa aggcttcgta
    19321 actttccaaa attttaatat gtttatcggc aaaattggac atataccatt cgtcagtgat
    19381 tggattagta tttaaatcat ctaaaaaaac atttaaatca tctaaaaaaa catttacatc
    19441 atctaaaaac atgactaatc cttaaactta aaatctgttt tctcaccggt tcttgagttt
    19501 tcaatgtaat gtatttcaat tttgtttacg ttttgaacac gcttaaccca accgtctttt
    19561 gctaaccaac catttttagt atcggacata ggaggtattc tcgcaggagt tttgccctgt
    19621 gggtttgcct taacttcttc cattgctaat tgttccttta aattattagg tattgtacga
    19681 ccggttgatt tttcaacaga ttgtgtccct atttttaatg atttgtttcc cccagccatt
    19741 tccccgtcta cagtctgcat agggcgggca gcaggttttt tatagccggt atttttaacc
    19801 caatcccatt tagtcgggtt aagttctact tttttaccgc gccaaaccgt acctgcggcc
    19861 tctgctacgg aaagggcagt ttgggctgtt gcagttatat tcggatgggc atcagcccat
    19921 tgtctggcgg aattaatgcc gttttttacc gcaaaagcac tgtcctgtaa taggctcgcg
    19981 gcagcaagtt gtgcttccgg acttaaattt cctaaatcat taataccttg tagagtctgc
    20041 tgagcggctg tatctgagcc ggtcagtgta ggttggatta aaacccagca ttttcaacat
    20101 cttgatgttt tactataaat ctattcttgt gcttcctttg ttagataaat ttcaacacaa
    20161 aagatacgta tgcattctct aattttattt gagataaata atgcattgct ttgtcgatac
    20221 tgttaaataa attacttccc tcgtaatacc aattatcata tgtttgataa aaataattat
    20281 ctttcttgat ataaatatct ccccccaata caagtattct ctcattgata aataaattta
    20341 atatatctat tgcaataggg acgggtaatg cggcttctct aattccaatt cctatcaaag
    20401 attccccttg tctcgaataa atttcaaaaa tttcatttaa catgttaatc tctatctact
    20461 atttttcaat cggtataaac cctattcaca gtttcatctt gtatttcttt atgaatactt
    20521 aatatattaa gatagcttag gaattttatt ttctcagatt gaggcaactc atcaaaacca
    20581 agccaacaat aaaaattgtt atatgatttt aaaaaatcaa caatttttat ttttcctaaa
    20641 accaaatctt cgactaactg ggttgcataa ataatatcat ttatgatttt aacaggtaaa
    20701 ttatttgata ggtaaccatg ggattccgtc atcattgatt tctctaggtg cttatttatc
    20761 tgattggtaa gccttttgcg taagcttctt tcgcctccgc aagtgtcatt tcattagggc
    20821 caccatctat attagtttct cctgtatttt gccactgaca aacgtcacag atatcatagt
    20881 ccataacttc agttccgcaa acaggacaat gtagccatat ttctccgtca atttcccatg
    20941 tcttttttga attctccatc ataatacttc tttcctaagt ttggtttaaa cattgttttg
    21001 attttgaatg tttttggatt ggccaaaaca tagatattgt tattgatatc atagcgcact
    21061 cgtctatgtt ccgtctgata acctaatatc ttgtccgatg tctgttttga taacaaatca
    21121 gacgccattt tttggtagtc ttcaatggtt atatcgccaa attcttttcc atgagctttg
    21181 aaatgtccat ttaaagattt ctcagtagga aacttggact ttgtccatct gatgcggtct
    21241 tgaattgttc ctgtcggaga aatagatgta tcaggtttaa catatgaggc aggtaagccg
    21301 atattgttat cagagtgtgt agggtgttgc tgcgtacttg gttttggtgg cttattccca
    21361 cctgccatct ccccatctaa agtctgcata gggcgggcag caggtgtttt atagccggta
    21421 tttttaaccc aatcccattt ggtcgggtta aggtttactt ttttaccgcc ccaaaccgta
    21481 gttgcggctt ctgctacgga aagggcagtt tgggctgttg cagttatatt cggatgggca
    21541 tcagcccatt gtctggcgga attaatgccg tcttttaccg caaaagcact gtcctgtaat
    21601 aggctcgcgg cagcaagttg tgcttccgga cttaaatttc ctaattcatt aatatcttgt
    21661 aaagtttgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat atttgacgcg
    21721 tctatcgttt ccgaaatgcc gctgttgaaa aacgtcggat tcgagaatcc gacctacggc
    21781 tgctaccaac catcattttc agcttctttt agccaagtat caaaattttc aaaagtctcg
    21841 taatattttg tattatctaa attaactact ataattttag gattacctga tttatctgaa
    21901 atatcaaaac aggcagccca ctcttggttt ctagcaaaag gtaataggtt tggaatgcca
    21961 gttatttcaa aataaatgtc aattacttct gatatattgc tttctgcatt ttcaaaccac
    22021 caaggaaaag aatattgttc atcgtagtta atggcatgag tggattgagc taattttaaa
    22081 taactttgcg gatatttaaa tcctttagga agcattgctt caggatacgg aacaaaacta
    22141 ttttgtttta tcatttccta agtaccttta catcataaat acttccgtta ggaaatcttt
    22201 tgctccaccc ttctggaact cctttttgtg tttcttgctt tctaattcgt agctggatag
    22261 cttctcccca agtttgaggc tcaataccat catgcttaaa cctgattctc tcttttgatg
    22321 ataatcggtc attaaaatta tgaacattcg cttccacttt tacccctgct tcacgtgctg
    22381 ccataatacg cgtgttatcc atactagtag gtgctccatc aggcatatta acaacatcaa
    22441 ccctgtcgcc aacccatgat ttttttcttt catttcggct acaatagaat catagttgta
    22501 atttgcacta ggctctgaca aaaatttggg ttgttaagtt gtcatttttt tataaattcc
    22561 agcaaactgt tgttgtaaat tatctacttc atgttttcta acaccagata cattttcaaa
    22621 ttctcttgaa tctacacata cgcccgcata tatctcattc aagatagctc gtaaaacaaa
    22681 aacttcatct tgagttagtg aaatagttga gttttgatta tttatggata gtatattcat
    22741 ggctttttcc tataaatcat cataatattt tttacctgat gttggtctaa atgctgtacc
    22801 tccatcgtca gaatttttat ctcggataac tattgtccct gttttatcat cccaatacgc
    22861 agttctaccg cgaggtaact ctttcatatt tgttggatgg ctaacaatat tttcaatatg
    22921 ccgagcaaaa tctgctggtg aattgatatt taaatccgta aattcttgtt gtcttatgac
    22981 atgcttgtta taagcatgcc cactactaat ttgttctcca attagttgcg cttgtaaaga
    23041 cggttgtgtg gaagcatctg ctttgctgtt ggacgttata gatttaggcg gtctattccc
    23101 ccctgccatc tccccatcta cagtctgcat atggcgggca gcaggttttt tatagccggt
    23161 atttttaacc caatcccatt tggtcgggtt aagttctact tttttaccgc cccaaaccgt
    23221 agttgc
//
LOCUS       DAWGJV010000012        22372 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWGJV010000012 DAWGJV010000000
VERSION     DAWGJV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.012"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(149..1990)
                     /locus_tag="WHQ94_000354"
     CDS             complement(149..1990)
                     /locus_tag="WHQ94_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ2127694.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALRA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2081..2551
                     /locus_tag="WHQ94_000355"
     CDS             2081..2551
                     /locus_tag="WHQ94_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ2127695.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(2623..3435)
                     /gene="hpnC"
                     /locus_tag="WHQ94_000356"
     CDS             complement(2623..3435)
                     /gene="hpnC"
                     /locus_tag="WHQ94_000356"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ2127696.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3522..4004)
                     /locus_tag="WHQ94_000357"
     CDS             complement(3522..4004)
                     /locus_tag="WHQ94_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2127697.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4477..4743
                     /locus_tag="WHQ94_000358"
     CDS             4477..4743
                     /locus_tag="WHQ94_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127698.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPASEAPASEAAASEAPAS
                     EAPAAEAPAAEAAASEAPAAEAPAAEAPAAEAAASEAPASEAAK"
     gene            4850..6217
                     /locus_tag="WHQ94_000359"
     CDS             4850..6217
                     /locus_tag="WHQ94_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ2127699.1"
                     /translation="MIDLHTEFSQLLPADEIIEPSPILLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCHKHRISVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELISHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELISRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPNSNLDGQLAEFLYEKGFTDSVLAQSEQERINMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYCNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            6252..6758
                     /locus_tag="WHQ94_000360"
     CDS             6252..6758
                     /locus_tag="WHQ94_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HEZ2127700.1"
                     /translation="MGMTATYQLVDNEKLALLKNTDTEWQELFEELEDLEDEAAVCLD
                     IDKMWDVLHFVLTGTSAENPLKGNLLSESIVGTAPISDGDGYAAYIESAQIHEIVSAL
                     EGFDIKSAMEHFSMQVCKEHHIYPDIWDYEDEIDDIKEEITDYFYKIGNFYKTARHSN
                     SNVLVSIY"
     gene            complement(7184..7630)
                     /gene="smpB"
                     /locus_tag="WHQ94_000361"
     CDS             complement(7184..7630)
                     /gene="smpB"
                     /locus_tag="WHQ94_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ2127701.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFLRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(7685..8695)
                     /gene="waaF"
                     /locus_tag="WHQ94_000362"
     CDS             complement(7685..8695)
                     /gene="waaF"
                     /locus_tag="WHQ94_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ2127702.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGLRGYDQVIVLPGSLKSAIIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPAKHFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINRLSDGICVNLCGKTDLSQAMDLLSLVDTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDKAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(8826..8901)
                     /locus_tag="WHQ94_000363"
     tRNA            complement(8826..8901)
                     /locus_tag="WHQ94_000363"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:Asn,seq:gtt)
     gene            complement(9092..9844)
                     /locus_tag="WHQ94_000364"
     CDS             complement(9092..9844)
                     /locus_tag="WHQ94_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ2127703.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERDEVQTALF"
     gene            10155..10922
                     /locus_tag="WHQ94_000365"
     CDS             10155..10922
                     /locus_tag="WHQ94_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ2127704.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKPLRQHRRRPVRSHIGGN
                     QFADLPHQVDAFVHGKTDAVMLQPRQHAAKRDLRRIVRHFFIRQHRRRIIKRRAHPPG
                     VRQLHRIAHCLRRPVLCGEPFYGFLFESNPLRLIEAV"
     gene            complement(11506..12651)
                     /gene="dapE"
                     /locus_tag="WHQ94_000366"
     CDS             complement(11506..12651)
                     /gene="dapE"
                     /locus_tag="WHQ94_000366"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ2127705.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(12764..13384)
                     /locus_tag="WHQ94_000367"
     CDS             complement(12764..13384)
                     /locus_tag="WHQ94_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ2127706.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(13409..13918)
                     /locus_tag="WHQ94_000368"
     CDS             complement(13409..13918)
                     /locus_tag="WHQ94_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2127707.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(14013..14564)
                     /gene="azu"
                     /locus_tag="WHQ94_000369"
     CDS             complement(14013..14564)
                     /gene="azu"
                     /locus_tag="WHQ94_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ2127708.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(14845..15267)
                     /locus_tag="WHQ94_000370"
     CDS             complement(14845..15267)
                     /locus_tag="WHQ94_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ2127709.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            15398..18148
                     /gene="secA"
                     /locus_tag="WHQ94_000371"
     CDS             15398..18148
                     /gene="secA"
                     /locus_tag="WHQ94_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ2127710.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            18292..20064
                     /locus_tag="WHQ94_000372"
     CDS             18292..20064
                     /locus_tag="WHQ94_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ2127711.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVIEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRTAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTTKQN"
     gene            20251..22179
                     /gene="rpoD"
                     /locus_tag="WHQ94_000373"
     CDS             20251..22179
                     /gene="rpoD"
                     /locus_tag="WHQ94_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ2127712.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         5847 a         6403 c         5257 g         4865 t
ORIGIN      
        1 aggattaagg gggtgtttgg gtaaaattag gcggtatttg gggcgaaaac agccgaaaac
       61 ctgtgtttgg gtttcggctg tcgggaggga aaggagtttt gcaaaggtct ccgtctgaga
      121 acttcagacg gcatttgaaa catcggaatc agcggttttg ttcataccac tcgataaact
      181 tgtctgcttt gacaaaaccc agcagcggct cgctgcggct gccgtcggag cggacgacaa
      241 atacgcccgg cggtccgaac agaccgtatt ctttcaacaa cgcctgatgt tcaggcgtgt
      301 tggcggttac gtcgatttgg aaaaagcgtt ccatatcaac cgcctgatgc acttccggct
      361 gattgagcgt gtatgccgcc atttctttgc aggaaataca ccagtcggca taaaaatcca
      421 aaacgacggg tttgtcggga tgttctttca acgccgtatc catcgctgcc ctcagcgcgg
      481 cagtatcggc aaacattttg ccgtgttccg aagatttgcc tgcttcggct ggtggattga
      541 gggtcaggaa atggtgcagc gcggtcgttt tgccgtttgc gccctgccag ccgaaccacg
      601 cgccgcctat cagcaatatg ccgcccaatg cgaatgccac agctttcgga cggcgtttct
      661 gcctgcgtcc gttgaccagc agcatacagg caggaaccag catcagcagc gtgtacagcg
      721 cgacgacgag ataatagggc aagtgcggcg tggcgaggta aacggcgacg gcaagcagga
      781 taaagccgaa agcgtatttg acggcattca tccaatcgcc tgccctaggc aggatatgcc
      841 cgccgaacgt gccgatggca atcagcggaa cgccggtgcc caacgccaaa gtgtaaagtg
      901 ccaaaccgcc taaaaccgca tcgcccgtct gaccgatgta gcccaaagca aatgccagcg
      961 gcggggcgac gcacggcccg acaatcagcg cggacaatat gcccataata aagacggaaa
     1021 cgattttacc gcctgaaagc ctgctgctct ggttttggaa atacgactgc acggcgttgg
     1081 gaagctggat gttgaacagc ccgaacatag acagtgccaa gacgaccatt aaagccgatg
     1141 ccgccaacac cacccaagcc tgctgcaacc atacggtcag cagcgcgccc gtcagtccgg
     1201 caacaatgcc gaccagcgta taagtcagag ccaaaccctg aacataaacg acggacagca
     1261 caaacgcccg cgccttgccc gcctttttgt cgccgaccac aatactggaa acaatcggca
     1321 acaggggata catacaggcg gtaaaactca gacccaaacc ggcgagaaaa aacgccaaaa
     1381 gattggcgtt gagcgtatcc caagacagct tgaaacggct gtcgccgccc tcatccccct
     1441 tcgggggcgg caacgccccg ctgccgtttt gagaggaagg ctgcaaaaag cggtctttgg
     1501 cggatgccgg ttcgtcggtt tgcggatggt aagtgccgtt gccggaaata tcaaactcgg
     1561 tatccacggg cggatagcac acgccggctt cggcacagcc ctgataggtc aaaaccaatt
     1621 tatacggttc gccgacagcc tttgcataag gaaaggcaac ctgcgcttcg tgatggtaaa
     1681 ccgtctgcct gccgaaaaac tcgtcttcct tctcttcgcc cttgctgaaa gaaggctgtc
     1741 ccaacaaatc cgccggatcg gtcttgccga cgattttcgc ctgatacata tagtatccgt
     1801 cggcaatcct gaaacggacg ttcacaccgt cgtcggcaac ggcaagctcc ggcacgaatg
     1861 ccttttccgg cggcagcaaa tcgttcgcat ccagcgcgaa agctcgtccg cacaacatca
     1921 aaaatacggc gaacaggcaa atcagttttt tcataatcga atccgtttca gacaaataat
     1981 ttgtctgcat tataaatggt aaggttgacg gtgggattta atttatgtaa aacccgccat
     2041 tatccgaacc tatttccata aacatcttac cgaacccgcc atgtacgatg tcaataccca
     2101 cgatgtccgc cgctttttcg cccgcgtgtg gcagcagcgg ctcaatccgc tgcaactgag
     2161 cgcactggaa cagaaagccc tccgcattat cgaagcccat cccgaatacc accgttatct
     2221 cgaacgcatc gaagaccatc tggacaccga ctggctaccc gaaaacggcg aaagcaaccc
     2281 cttcctgcat atgtcgctgc atttgtccgt ccaagaacag gcgggcatag accagccgca
     2341 cggcatacgc gcaatccacg acaccctgtg cgccaaacgc ggctggctgg aagccgaaca
     2401 cgaaatgatg gaggcactgg cggaaacact gtggacggcg caacgctacg gcaccggttt
     2461 ggatgtcaat ttctacatga cccgactgcg caaactcatc ggcttgggtg cagaagatca
     2521 agccaggttg aacccgcatg aaatcgcctg accataccaa ccgcctgcaa aatgccatct
     2581 gaagcggaac aacccctttc agacggcatg catttccccc aatcatttcc acaacgcctt
     2641 tttcagcata atcaaccaat ccttcttatc caaaacgggg cgttgtgcaa acacatcgta
     2701 tcggcacgcg tccagtttct gcaaaatcaa ctgcgccccc aacacaatca tacggagttc
     2761 caaaccgata cgccccttca gttcccttgc caaaggcgaa cccgccttca gcatacggaa
     2821 tgcgcgtcgg cactcatacg ccatcagccg ctgaaacgcc gcatccgccc gtcctgccgc
     2881 gatctgttcc tcagaaacac cgaatttcaa caaatcgtcc tgcgggatat aaaccctgcc
     2941 cttttgccaa tccacagcca catcctgcca gaaattgacc agttgcaaag ccgtacaaat
     3001 gccgtcgctt tgcgccacgc acaccgcatc cgttttcccg tacaaagcca gcataatgcg
     3061 tccgacagga ttggcagaac gtcggcaata atcggccagc tcgccgaaat ttccatacct
     3121 tgttttaacc acatcctgag aaaatgcaga aagcaaatca taaaacggct gcaaatccaa
     3181 accgaacggc acaaccgcct cggcatccaa tcgtgcaatc aaaggatgcg ccgaccggcc
     3241 gcccgatgcc aacacgtcca actcgcgccg caaaccctcc aaccccgcca acctggcttc
     3301 agacggcata ctgccctcgt ccgccatatc gtccgccgtc cgtgcaaacg cgtacaccgc
     3361 gtgaaccggc ttcctcaacc tgcgcggcaa aatcagcgaa ccgacgggaa aattttcata
     3421 atgcccaacc gacatacctt ctccatccat caaacaaaat gccgtctgaa atggaacaaa
     3481 cccttttcag acggcatcag atacctccaa gctgccggca atcagtggtg gtgatgaccg
     3541 tgcgggccgt ggacatgacc gtgtgcgatt tcctcatcgg atgcatcgcg cacactttca
     3601 accgtagcct taaagcggat tttcatgcct gccaaaggat ggttgccgtc caccaccgcc
     3661 ttaccgtcgg caacatcggt tacacgatag acgacaacat cgccggtttc aggatcgtcg
     3721 gcttcaaaca tcatgccgac ttcgacttca acagggaaca cgcccgcatc ttcaatacgg
     3781 accaactccg gatcctgctc gccgaacgca tcgtcgggcg aaagcgccac atcgaccgta
     3841 tcgccggcat ccttaccgtg caacgcctct tccaccaaag ggaaaatgcc gtcgtaaccg
     3901 ccgtgcagat acgcaatcgg ttcttcggtt ttgtccaaaa gctgattgtt ggcatcatac
     3961 atctcataat gcagcgaaac cacggaattc ttcacgatag ccatatttgt cctttcagga
     4021 acagcagatt gattacaggc gcattctaac acaaccgccg cgccggccga ttaccgttaa
     4081 cctgttcata aactgtacag cacatatttc aatgtaaatt tttgttattt tattgcgatg
     4141 taactttttt acaacattat taaaaccatt ccgacctgtc tgccgacttt cccaatccgc
     4201 cttaataaat catacaagat actgaaatta tattaatctc tataatattt atccctatcg
     4261 aatttttaac agcaaaaccg ttttacagga tttatcaatc cgcccgccag aaaacttttc
     4321 attcaaacct ttttcccatc tgtacgacat tgcaatccct tattccatag tgcataatta
     4381 cgcaaattca gcgatgaatt tccaacccgg tttgtagtat ggtcgataaa gacctatttg
     4441 tttcaataat ttaaattggt tctaaaggtt actaaaatga aaaaatccct gtttgccgct
     4501 gctttgttgt ctttggctct ggccgcctgt ggcggagaaa aagctgctga agctcctgct
     4561 tccgaagctc ctgcctctga agccgctgct tctgaagctc ctgcttccga ggctcctgcc
     4621 gctgaagccc ctgctgccga agctgctgct tccgaagctc ctgctgctga agctcctgct
     4681 gctgaagctc ctgctgccga ggctgctgct tctgaagctc ctgcttctga agctgcaaaa
     4741 taagcatttt ctgcttgcaa aaaagcagga tactttgcgt atcctgcttt tttaattttc
     4801 cggacgcatt taatcctatt tctcaatctc cactcttttt cccaaaaaca tgattgacct
     4861 gcataccgaa ttttcccaac tccttccggc agacgaaatc attgagcctt cccctatact
     4921 tttaaaagac cagcgcaacc gctttacgtc tgcaccagac atcattttgc agccgcgcag
     4981 cgttgaaagc gtgcaaacca ttatgcgttt ctgccacaaa caccgtattt cggttacgcc
     5041 gcaaggcggc aataccggtt tgtgcggcgc ggcagtatcg gaaaacggcg tattgctgaa
     5101 cctttccaaa ctcaaccgca tccgcagcat caatttgtca gacaactgca taaccgtcga
     5161 agcaggttcc gtactccaaa ccgtccaaca ggcagccgaa gcctcaaaca ggctgtttcc
     5221 actcagcctc gccagcgaag gctcgtgcca aatcggcggc aacatcgcct gcaatgccgg
     5281 aggtttgaac gtattacgct acggcacaat gcgcgacctg gttatcggtt tggaagtcgt
     5341 cctccccaac ggcgaactga tttcccatct tcacccgcta cacaaaaaca ccacgggtta
     5401 cgatttgcgc catctgttta tcggcagcga aggcacactg ggcatcatca ctgccgccac
     5461 gctcaagctt tttgcccgcc ccttagacaa agcaaccgca tgggtcggca tacccgacat
     5521 cgaatccgcc gtccgcctgc tgaccgaaac ccaagcacac tttgccgaac gcctatgtag
     5581 ttttgagctg atcagccgtt ttgccgccga gttgtcttcc gaattcagca aactccccct
     5641 gccgacacat tcagaatggc atattttact tgagttgacc gactctctgc caaacagcaa
     5701 ccttgacgga caacttgccg agttcctgta cgaaaaaggc tttaccgaca gcgtattggc
     5761 acaaagcgaa caagaacgta tcaatatgtg ggcgttgcgc gaaaacatct ccgcttcaca
     5821 acgcaaactg ggcaccagca tcaaacacga tattgccgtc cccatcgggc gcgttgctga
     5881 ctttgtccgc cggtgcgcca aagatttgga acagaatttc aaaggcatac aaatcgtctg
     5941 cttcggacat ctgggcgacg gcagcctgca ctacaatact ttcctgcccg aaatcctcag
     6001 caatgaagtc tatcgttacg aaaacgacat caacagcaca gtctattgca acgttcttgc
     6061 ctgcaacggt acaattgctg ccgaacacgg cataggtatc atcaaaaaac agtggctgga
     6121 caaagtacgc acacctgccg aaatcgccct gatgaaaagc atcaaacaac accttgatcc
     6181 atataacatc atgaacccgg gcaaacttct tccataaata actaattcaa aatgaaatta
     6241 taggaaatat catgggaatg acagcaacct atcaactggt cgataatgaa aaactggctt
     6301 tgcttaaaaa caccgatacc gaatggcagg aattgtttga agaactggaa gatttggagg
     6361 atgaggctgc cgtctgtctc gacatcgata aaatgtggga tgttttgcat tttgtactga
     6421 ccggaacaag tgcagaaaat cccttaaagg gcaatctgtt atccgaatca atcgtcggaa
     6481 cggcacccat ttcggacggc gacggatacg ccgcttatat cgaatccgcc caaatccatg
     6541 aaatcgtctc agctttggaa ggcttcgaca taaaaagcgc aatggagcat ttcagtatgc
     6601 aggtgtgcaa agagcatcat atctaccccg acatttggga ttatgaagat gaaattgatg
     6661 atataaaaga agaaattacc gactactttt acaaaatcgg caatttttac aaaaccgccc
     6721 ggcattcaaa tagcaatgta ctggtcagta tttactaaat atatttcaaa caccacggaa
     6781 actttaataa agacaaagta cggaatcgca ctcaaggaaa cctaggtatt tcaagccggg
     6841 agaccatcaa gagtaaaagt tacagaaaac accccttttc ccctataacc acggcagtca
     6901 gcgaaaaaca ggacaaacgt cttgctaacc agaaactacg gagaatagcg cgcgaatgtc
     6961 tgaaacaagg aaaagaacca ccccaccata tcaaggcagt cagcaacgtt tacgatttcg
     7021 ccaaagacgg gcgtttccgt ttttccgctt cccgtcatcc gcatctttta cgcaaataga
     7081 aaacagggca accgccctgt ttttccgttt cgtatttcaa caccattgtc caaacaaccg
     7141 tcagacggca ttgttcaaat gccgtctgaa accgcatccc ccatcagcgc gtatgcttaa
     7201 tcaaccgctg tttctcgcgt ttccagtcgg cttccttcat actctgtcgt ttatcgtgct
     7261 gtttcttacc ttttgccaaa ccgatttcca tcttgatttt tccgcgcaaa aaatgcaaat
     7321 ccagcggcac gatagtgtaa ccggcacgtt cggtttttcc gattaatttg ttgatttccg
     7381 actgtttcaa caagagcttg cgcgggcgta cggcatccgg tttgatgtgt gtcgaagccg
     7441 taggcaatgc ggtaatatgg cagccgacca gataaaacgc atcttttttc caatagatat
     7501 aactctcttt aagctgtacg cgcgcggcac ggattgcttt gacttcccag ccttccaata
     7561 ccaaaccggc ttcaatccgg tcttcaataa aaaaatcgtg aaatgctttt ttattattcg
     7621 caatagccat aaacatccta tcaatatccg ccgtcagacg gcataaaccc gaaaacagaa
     7681 cccatcatac cgcctcttca accgcctgca caatcttctc gggatacagc ctgttgaggc
     7741 agtcggtatg ccccagcgga cattcccgtt tgaaacacgg ggaacattcc aagtgcaggc
     7801 tgacgatttt tgccttatcg cttaaaggcg gcgtatgcgt cgggctggaa gaaccgtaaa
     7861 ccgccactac cttcctgccc aaagctgccg ccaagtgcat cagcccgctg tcgttacaca
     7921 caaccgtatc taccaatgac aacaagtcca ttgcctgaga caaatcggtc ttaccgcata
     7981 aattcacaca tatgccgtct gaaaggcggt tgatttcctc ggcgatttca tcatcttttt
     8041 gcgaaccgaa cagccaaacc tgccaacccg ccgccgaata atgtttgccc aactcggcaa
     8101 aatgctttgc cggccaacgc tttgccggtc cgaattccgc acccggacaa aaagccaaaa
     8161 caggctttcc aatatccaag ccaaaggttt cgacagaaat ttcccgccgc cgttcatcaa
     8221 tggaaaactc ggggaatccc gaatgcccgt caaaatcttc ctgactctga tgcgcgagag
     8281 ccgtatatct gtccaccatc aaaggcagac gttccttatc cagcctgcgt atatcgttca
     8341 acagaaaata acggctttcg ccgacataac ccgtcctttt ggcgataccc gttgccagcg
     8401 cgatgattgc cgatttcaaa gaaccgggca acacgataac ctgatcgtat ccgcgcagcc
     8461 ccaaatccct gccgacccgc cagcggcgtt tcaactccaa cgcaccatgt ccgaacggat
     8521 tttcaagaat ctcattcact tcaggcatac gctcaaacac cgccatcgac cacttcggcg
     8581 cgaacacatc aatcgtgcaa ccgggatgaa gttccttcaa acggcggaac aagggctggg
     8641 tcatcacgca gtcgcctatc caactggggg aaataatcag gattttgatg gacataacaa
     8701 gaaaccgaaa tcagacaggc agaattttac cgcgaaaccg ttggaaaacc tatcttgccg
     8761 cattccgaac gccggacgtg caaatatgaa aaagcccgaa cattcaagtt cgggcttcaa
     8821 aattctggct ccccgacctg ggctcgaacc agggacctgc ggattaacag tccgtcgctc
     8881 taccgactga gctatcgggg aatggggcgt attatagcgt ccggaaaaaa tgtgtcaatc
     8941 cttaattttg gaaaaatggg cgacaaaacg acaagcatat gaatcagaaa gacattaaga
     9001 ccgatgcctt aaaaggattg ccgttgtatg aatttccaca gccgtcatca caccatattt
     9061 aagcccagtg aaccgttctg catttccccg cttaaaacaa tgccgtctga acttcgtcgc
     9121 gttccaaagc cagtaaaaac tgtttgcggt tcaacccgcc tgcgtaaccg gtcagtttgc
     9181 cgtcgctgcc gatgacgcgg tggcagggaa tcagaatgga caccttgttc tgcccgttgg
     9241 cggcggcaac ggcgcggacg gctttggggt tgcccaaacg ctgcgcctgc tccttataac
     9301 tgcgcgtttc gccgtaagga atcgccaaaa gtgcgtccca cgcctgcttt tgaaactcgg
     9361 tgccaatctg ctccaaaggc gtggcaaagg ttttcagacg acccttgaag tataagtcca
     9421 attcctgccg caaaagttgc gtccgctcat cctcccgaaa cacaaaccgt ccgcgcaagg
     9481 ctttttggac ggcggcaatt tcctgttcca aatgcttctg tccgacaaac tccagcagac
     9541 acaatccctt atttccgaac accgccagca tctcgcccaa aggcgtggca atggcggcac
     9601 acaccagctc attcaaactg tcgggataac gcgcttccaa cagacggatg gctcggcgga
     9661 tacggacata ttcttcaggc gcgcagccga tattgtccca aaaatcccga tcgaactgcc
     9721 cggcttcgca ttccgtcaaa ttgggatgcg gcataacgcc gcactcacaa acccgggttt
     9781 cgagccaata gcggatttca tcccattttg acggcagatt gtttaaggaa ggcagggtaa
     9841 tcattcgttt gctccgtatc tctatcatgg atttggcggc aaaatcccca atttttgcca
     9901 ttcccgcacg ccggagcagg aacgggctat gacgtaaatc ttgagggtta ggttgcggca
     9961 atacctaaat attcgatatt tctaaagcct cagggaaagg aattaaagac ttcagacgac
    10021 ctgcaaaggc tcgtctgaaa ccacgatttt tgcatttgcg cattccggca catcatccaa
    10081 ccgtttcggc acattcctgc cgccgttgac agcctataat gaatccactt attcatcaag
    10141 caaaggaacc gtctatgcaa acccgcattc tctccgccgt actgctggct ttttcaaccg
    10201 ctgcctttgc cgggggcgca ttcacgctgc aattcgacaa cccgtccgaa gacggcggct
    10261 tcacgcaaaa ccagcttttg agcgcgcctt acggatttgg ctgttcgggc ggcaatgctt
    10321 cgcccgcgct gtcgtggaaa aatccgcccg ccgggacaaa aagtttcgtc ctgaccgttt
    10381 acgataaaga cgcgccgacc ggactgggct ggatgcaccg ggtggtcgcc gacattcccg
    10441 ccgatgtcca ccgccgcaac gcgacctcgc tgcaattaag ccgctgcgcc aacatcgccg
    10501 accggactgg gctggatgca ctgggtggtc gccgacattc ccgccgatgt ccgccgccgc
    10561 aacgcggcct cgctgcaatt aagccgctgc gccaacatcg ccgacgacca gtccgcagcc
    10621 atatcggcgg taatcagttt gcggatttgc cgcatcaggt tgacgccttc gtacacggca
    10681 aaaccgatgc cgtcatgctg caaccacgcc aacacgccgc aaagcgcgac ctccgccgca
    10741 ttgtgcggca cttcttcatc cgtcagcacc gccgccgcat aatcaaacgc cgcgcccatc
    10801 cgcccggggt gcggcagctt caccgcatcg cacactgcct gcgccgtccc gtattgtgcg
    10861 gcgaaccttt ctacggtttc ctgttcgaaa gcaatccatt gcgcctgata gaggccgtct
    10921 agatcgggaa tattaataac gtcaaacgtc tgtccgcctg ccaacgcgac cgcctcaccc
    10981 gccgcagctt cttacttccg cgccgcacga taagcacagc cggttcatat accgccacgc
    11041 tgcggtacaa ggcggtatga tgttgcacga tgccgcccaa agcacccaat cgtccgcgcg
    11101 tatataaagt aatttgacgg cacggttata ttcctcaatg tttcaacaca cagggccgca
    11161 cataaggcac agccctgttt accctgattt ggaaggggtt acttcccaaa aaataaacca
    11221 aaatcccatc gcccagaagg gccgggtttc aaccatattc cgtcggcgtg cctgcaactt
    11281 gactccctcc cctgtggggg agggttgggg agagggtaaa acggggcaga tacagacaat
    11341 atttccgtta ccgccccgat gccctcccct aaccctctcc cacgagagag ggaatggatt
    11401 gccgttgaaa taaatcgctc tacataaaaa atcaatgtgt tatctcaaac ccacgttagg
    11461 tctaagcaaa tggtcggaca tccgtatccg gcaagcaagc tgctttcaga cggcatttcc
    11521 agccaacaac cgcgccaata tcccctcata caccgcagac agcttcggaa tgtcgtttag
    11581 ccgcacgttt tcgttgattt ggtggatggt cgcattggac gggcctaatt cgatgagttc
    11641 ttttgcaatg gctttgatga agcgtccgtc cgaagtgccg ccggtggtgg acaattcggt
    11701 ctctacgccg cagatttcgg caatggcggc gcgtgccacg tcggtcagtt tgcccgcgtg
    11761 ggtcaggaag ggctgccccg aacacgacca ctgcaaatcg tattgcacac cgtgtttgtc
    11821 caaaatggcg tggacgcgtt gtttcagccc tgcttcggtg gactcggtgg agaagcggaa
    11881 attgaatttg acgctcagct cgcccggaat gacattggtc gcgcctgtac cgccgttgat
    11941 attggaaatt tgaaagctgg tcggcgggaa gtatttgttg ccttcgtccc agacttcctg
    12001 cgtcagctct aacaaggccg gggcaaaagt atgcacggga ttgattgcca aatgcggata
    12061 ggcaatatgg ccttgcttgc ctttgacggt caggttgccc gacagcgagc cgcgccggcc
    12121 gtttttaatc atatcgccca atttgtccac ggctgtcggt tcgccgacga tgcagtagtc
    12181 gataagctcg tcgcgcgctt tcaatacatc gacgactttg gtcgtgccgt ccaacgcgtc
    12241 gccctcttcg tcggaagtaa tcagaagcgc aatgctgcct tggtggtcgg gatgttcggc
    12301 aacgaagcgt tcacaggcgg taacgaagca ggcgatgctg gttttcatgt ctgccgcgcc
    12361 gcgcccgtat aatcttccgt cgcgctcggc cggttcgaac gggggcgaat cccatttttc
    12421 gacaggacct gtcggtacaa cgtcggtatg ccctgcaaaa cagacgacgg gagctttcgt
    12481 gccgcgtcgc aaccagatgt ttttggtgtc gccgaaatgg agttcttcag ccgcaaaacc
    12541 gattttgtgc aggcgttcgg caaggagttt ttggcaatcc ctgtcgtcag gggtaacgga
    12601 tggtcgggaa atcagctctt tggcaagctc tagggattga gtttcggtca tatttgttca
    12661 cttttgaaat tagaccgtct gaaacgttct gaatgtgatt ttcagacggc atttaggtta
    12721 ggttggcata cggggtgggt attttaccca tcagtcttct gaatcatttg ccgtggcagg
    12781 cttcgtaaag cggcagcaaa tcttccaccg tttccgctat ccatttcgcg acatcctgct
    12841 tgcccaaatc gtcgcgctcg atgtgtttgc cgatgcagaa aaagtcttcg tcgttttgca
    12901 actttcggtc ggattcgctt tgttgtgcga cggtgcggta gtcgtcatat tcgctttccg
    12961 cgccgtgcca catatcgaat gcggcgtatt tgtcggtatc gaaatcatcc aaccagcggt
    13021 tgtattcggg cagcgcaatc ggcgacacgt cggctttgta gcagtgccaa tccaagctga
    13081 cgctcaggcg gcggcggttc aataaaatcg ataaaatcgc ggcggaattt ttatattgtt
    13141 cgtatttgaa gtaggcaaag aaatgggcgc gcacctgcca gccgttacac cagcgttcga
    13201 tatgcggcgg cgcgaacggc gcacccaatt cggcggcaac ctgctggatc agctgctgcc
    13261 atacctgcca gttttcttta tagtctgcct tgatttgcgg aatgctttca ggctggtatt
    13321 ttttaagctg ggaaaattgg aaaaacggga tattgaacaa atcgcaactt ttcgaggtaa
    13381 gcatagtgta ttccttgaga cgattacctt atttgcgccg acgcgccgcc atgatttcac
    13441 cgatttcggt cagtttttct ttagggataa aggtgttgcc catatcaaac agcggctctt
    13501 caatcgccaa atgaacatca tatcccgcca caaaacgttt gaacgcttcc gcatcgggaa
    13561 cataagcatt gtctgcttcg agcttggcaa attcggcgga aacagccgcc cagttgtcgt
    13621 gcagcccgat atgttggcgt aaaagctcgt ccacaccttc acgtgcttgc ggcgcgtatt
    13681 gcagcagcag cgggaaaaag tcttcttctt cgtcctcatg atgcagcggc gcggcaacgt
    13741 tgaaataccg ggtgatttgg cggatggttt gcaaaacaat ttgattgcag ccgttttcgg
    13801 cgatgtagtc cgacagcatg gcgacttgtc cgcaaaaacg gcgcactttg ccgtggcagg
    13861 catacagcat ttcaatcggt tcggcaaagg taacgctttt ggtttcaaac ggattcatgt
    13921 tttcgttctc aacgggcact tttcaagcag tcattttata ataaaacagc ctgcacaaag
    13981 caggctgtcc gtcttttgag actttaagcg gattaatcga ccaaagtcac tttgccgttc
    14041 atcaaagcac cgtgacctgg gaaggtacaa gcgaatttat attcgccgtc ggccaattta
    14101 gcaggatcca gagtcaggga agcttcttcg ccgccgccga tcagtttggt atgggcaaca
    14161 acgcgtgcat catcaggttt gacatagtcg gtatcggcag cacctacgcc gtctttaaat
    14221 acgccgtcca tgtcttcagc tttggcaatc acgagattgt gacccatgct ggctttgggt
    14281 tgcgtaccgg tatgtttcag agtgatggta aactctttac atgctttgct gacttggatg
    14341 tctttggtgt tgaactgcat attgtcgttg gattcgacag ttgccgcaca gttgccgaca
    14401 gcaggggctt cggcagcatc tgcaggagca gcttcggcgg caggtgcttc ggaagcgggt
    14461 gcttcagcag caggagttgc ctcggcagca ggcgcggcag gttcttgaga gcaggcagcc
    14521 aaaccgataa cggcagcaga aatcagagcc agatacgctt tcataacaaa tctccaatcg
    14581 ataaaataat attcggtttt acagaaatca aagtgcaacc gccattaaca aaaccttgaa
    14641 aaagattccg ccgcgttgca caaacagatg tttcggagcg gcattttgct acaaatttca
    14701 tttgaaatca aagcctgtct gcaagtttac aatcgtttac tttaaaaggg gcaattttac
    14761 cccgaaccta tttctttagt attagaccca ttatccttta cttcttaata ttaacggatg
    14821 tttacacaaa ttcccgtata cattttatgc gccatgcctt ctaaccaagt ttgccaatgc
    14881 ctccgccaat tcgggatgcc gtttttccaa ctttaccgcc gccgaaccga aactctccag
    14941 cgcagcctta ctcaaatgca gggtattggt tttcggcggt ttttccggtt tcgggaccag
    15001 cctgaccgaa acagagcgta tcgaagcgtc aagccctgcc aactgcggca ataccgacgg
    15061 tgcaatcatt ttcaagcgcg atgccgccat attgtttgcc gccaaaagga caagcctgcc
    15121 gtcttcgata cataccgtct gaaaatgcgg gtgcaggttg gcaggcagca gttttttcac
    15181 ggcggcatcc aaccgccgcc actgtcccgc ctgtttcaaa agtccggaaa gcagcgcgtc
    15241 ccgcctgccc aactgttcca aattcataaa acatacaccc aaaaagattg aaataccgca
    15301 aacgcgcctt tatttcagac ggcattagca ctttgcacaa acgcttgtgt taaaatcgcg
    15361 ttttcgccca ctattatatc aggcgcagga attattcatg ctgacaaaca ttgccaagaa
    15421 aatcttcggc agccgcaacg accgcttgct gaaacaatac cgtaaatccg ttgccagaat
    15481 caacgcgctc gaagaacaga tgcaagccct aagcgatgct gatctgcaag ccaaaactgc
    15541 cgaattcaaa caacgcctcg ccgacggtca gactttggac ggcattttgc ccgaagcctt
    15601 cgccgtctgc cgcgaagcgt cccgccgcac cctcggtatg cgccacttcg acgtgcagct
    15661 tatcggcggt atggtgctgc acgacggcaa aatcgccgaa atgcgtaccg gcgaaggcaa
    15721 aaccctggtc gccaccctcg ccgtctatct caacgcgctg gccggcaaag gcgtacacgt
    15781 cgttaccgtc aacgactacc tcgcctcacg cgatgcgggc attatggagc cgctctacaa
    15841 tttcctcggc cttaccgtgg gcgtgattat ttcagatatg cagccgttcg accgtcaaaa
    15901 cgcctatgcc gccgatatca cctacggcac caataatgaa ttcggcttcg actacctgcg
    15961 cgacaatatg gttaccgacc aatacgacaa agtgcagcgc gaattgaatt ttgccgttgt
    16021 cgatgaagtg gattccatct tgattgacga agcgcgcact ccgctgatta tctccggtca
    16081 ggcggatgac aacatccagt tgtaccaaat catgaacacc gttccgcccc acctcgtccg
    16141 tcaagagaca gaagaaggcg aaggcgacta ttgggtcgac gaaaaggcac atcaggtcat
    16201 cctgagcgaa gcaggtcacg aacacgccga gcaaatcctg acccaaatgg gattgctggc
    16261 agaaaacgac tccctctatt ccgccgccaa tatcgccctg atgcaccacc ttatggcggc
    16321 attgcgcgcg cattccctct tccacaaaga ccaacattac gtcatccaag acggcgaaat
    16381 cgtcatcgtg gacgaattca ccggccggct gatgtccggc cgccgctggt cggagggtct
    16441 gcatcaagcc gtcgaagcca aagaaggcgt ggaaatcaaa cgcgaaaacc aaacccttgc
    16501 ctccatcacc ttccaaaact atttccgcct gtacaccaag ctctccggca tgaccggcac
    16561 agccgatacc gaagctttcg agttccaaag catctacaac ctcgaaaccg tcatcatccc
    16621 gaccaaccgc cctgtacagc gcaaagactt caacgaccag attttccgtt ccgccgaaga
    16681 aaaattcgaa gccgtcgtca aagacatcga agaatgccac aaacgcgggc agcccgtcct
    16741 cgtcggcacc accagcatcg aaaactccga actggtctcc cgccttctgc aaaaagccgg
    16801 actgccgcac aacgtcctca acgccaaaga acacgaacgc gaagccctga ttgtcgccca
    16861 agccggcaaa gtcggcgcga ttaccgttgc caccaatatg gcgggacgcg gtacggacat
    16921 cgttttaggc ggcaacctga agcaccaaac cgatgccatc cgtgccgacg aaaccttgag
    16981 cgacgaagag aaacaggcac aaatcgccgc actcgaaaac ggctggcagg cagaacacga
    17041 taaagtaatg gaagcaggcg gcctgcacat catcggtacg gaacgccacg aaagccgccg
    17101 tatcgacaac caattgcgcg gacgttccgg ccgtcagggc gaccccggat ccagccgttt
    17161 ctacctttcg tttgaagacc cgctgctgcg cttattcgca ctcgaccgcg ccgccgccat
    17221 cctcaaccgt ctcgcccccg aacgcggcgt cgccatcgaa cacaacctgc tgacgcgcca
    17281 aatcgaaggg gcgcaacgca aagtcgaagg cagaaacttc gatatgcgca aacaggtttt
    17341 ggaatacgac gacgttgcca acgaacagcg caaagtcatt tacagccagc gcaacgaaat
    17401 tctgaccagc aaagacatca gcgacctgat gcaggaaatc cgttctgatg tcgtcagcga
    17461 cctcgtggat acctatatgc cgcccgacag catggaagaa caatgggaca tcccgacttt
    17521 ggagaaccgt ctggctgccg aattcagact gcacgaagac atccaatcct ggctgaaggc
    17581 ggacaatgcg attgacggtc aagacatcaa agaacgcctg atcgaacgca tcgaaaacga
    17641 atatgccgcc aaaaccgaac tggtcggcaa gcaggcaatg gccgatttcg agcgcaacgt
    17701 gatgttgcag gtcatcgaca accaatggcg cgaacacctc gccgctatgg actacctgcg
    17761 acaaggcata cacctgcgca gctatgccca aaaaaatccg aagcaggaat ataaacgtga
    17821 agcctttacc atgttccaag acctgtggaa cggcatcaaa ttccatattg cctccctgct
    17881 tacctcggtt caaatcgaac aaaaccctgt cgcggtggtt gaagagcaac ccatcggcaa
    17941 catccagtcc atccattccg aatcgcccga tatggaagaa cttttgggtc agtcgcaaac
    18001 cgatttggtt accgaggcct ttaatcccga tggaacggat ttcagccccg aagccttgga
    18061 agcgcgggga caaatcgtcc accgcaacga cccctgcccc tgcggcagcg gtttgaaata
    18121 caaacaatgc cacggcaaac tggcttgagc gtttgaacgc aaatgccgtc tgaacatccg
    18181 gtttccggct tcagacggca ttttgcctga accgccacat ccgaccgcca ttccgaaaaa
    18241 tcccgatttc gtaccgtcca aactaaaaac aggcatccgc cccgccgaac tatgattcca
    18301 tccgacttca ttgacgaact tctggccaaa accgatattg tcgacatcat cgacgaacag
    18361 gttccgctga aaaaaggcgg ggcgaactat atggcgtgtt gcccgttcca caaggaaaaa
    18421 acgccgtcgt tttcggtcag tccgaccaag cagttttacc actgtttcag ttgcggggcg
    18481 cacggttcgg cgattggttt tgtgatagaa catcaggggc tgtcgtttcc ggaggcagtg
    18541 cagttccttg ccgaccgcgt gggtatgatt gtgcctaaag tgcgcgggca gaacgataat
    18601 cccgaagtcc gtgccgaacg taagaaaaaa cagcagacac tggaggaaac gacggctgcg
    18661 gcagctgatt tttacgcgca acagctaaaa ttcaatccag cggcaaaagc ctatttggac
    18721 aagcgcggtt tgagtgcgga agtcatcgcg cattatggtt tgggctatgc gcccgacggc
    18781 tggcagcctt tggcgcaagt gttccaaccg tacccgaata ccgcgttggt ggatactggg
    18841 atggtgattg acaatgaggg gcggtattac gaccgattcc gtcatcggat tatgtttcct
    18901 atccgcaatc cgcgcgggca ggtcatcggt ttcggcggca gggtgttgga cgactccaaa
    18961 cccaaatatc taaactctcc cgatacacct ttgtttgaca aagggaaaaa cctttacggc
    19021 ttgtatgagg ggcgtaccgc tgtcaaggaa gcggggcgga ttctggtggt cgaaggctat
    19081 atggacgtgg tcgcgctggc acagttcggc gtgggctacg gtgtggcggc cttgggcacg
    19141 gcaacaacag cggaacacgt caaaatcctg atgcgtcagg cggacagtat ttatttctgt
    19201 ttcgacggcg acagcgcggg gcgcaaagcg gcttggcgcg cgctggaaaa cgcgctgccg
    19261 cagttgaagg acgacaaatc gctgcatttt ttgttcctgc cggaagaaca cgaccccgac
    19321 agttacatcc gtgcctacgg caaagcgcaa tttgaagatg cgctgttgaa tcaaagcaag
    19381 cccttgtcgg aatatttctg ggaacacctt tcagacggca ttcatctcaa tacgcaggaa
    19441 ggcaaggcgg aattggtgaa aaccagttcg ccgcttttgg cgcagattac cgcgccggca
    19501 ttggcttatt tgttaaaaca acggcttagc gagctggtcg gcatcgaccc cgacaacctc
    19561 gcgcaactgc tgggacagga agcgccgaag cggcacgtca aacaaaaaaa ctacaaactg
    19621 ccgccgattt ccgtcaaaca gcccgttatg ccgacattgg tgcaacggca aatccgcagc
    19681 ctcttgataa atccggattg ggctgcatat atagacctgc ccgattatct ggcgttggac
    19741 ggtgatttcg cctgccttgc caatcttgcc gaatcgatta aaaaccatgc cgccgtaccc
    19801 gaaaccgctc aggttttaga gtatatgcgc ggctcgcctt acgaagaaac gataacccga
    19861 atcttccatt caacgcacca atcggaagaa atgaacagca gcagtgaaga agattgcgag
    19921 aatttccaaa tcggcatgaa aaaactgctc aatgagttaa aatacagcca aatcgaagca
    19981 ttgaaacaaa aaagcctgca atccggctta aatgaaagcg agaaaaaact tttgctgtcg
    20041 ttgctgacca caaaacaaaa ttgaccggcg gattccgcca cccgtaaacc cgttatgccg
    20101 tctgaaaagc acttaccccg gctgcaacaa cgacacctgc agaacaccca tcccaaaaag
    20161 ccttcagacg gcatcagagt acccgctctg ccacgccttc aggtgcgttc aaacgcaaac
    20221 cgtcggcacc ttaccaacag aaagcagaca atgtccagaa accaaaatca cgaagaatac
    20281 caagacgatg cccgccccct gacgattgag gaacaacgcg ctcgcctgcg ccaactcatc
    20341 attatgggca aagaacgcgg ctacatcacc tactccgaaa tcaacgacgc actgcccgac
    20401 gatatgtccg atgccgatca aatcgacaac atcgtcagta tgatttccgg tttgggtatc
    20461 caagttaccg aacacgcccc cgatgcggaa gacatattgt taagcgacaa tgccgccgtt
    20521 accgacgatg atgccgtcga agaagccgag gccgcccttt ccagcgcaga ttccgagttc
    20581 ggcaggacga ccgaccccgt ccgtatgtat atgcgcgaaa tgggacaggt cgacctgctg
    20641 acccgcgaag acgaaatcat catcgccaaa aaaatcgaaa acgccctgaa aaatatggtt
    20701 caggccatct ccgcctgccc gggttccatt gcggaaatct tagaactcat cgaaaaaatc
    20761 cgcaaagacg aaatccgcgt cgacgaagtc gtagaagcca ttatcgaccc gaatgaagta
    20821 ttgctcaacg aattgggctt ggggcacttg gaaaccacag cacccgagaa accttccaac
    20881 gacaattcgg acgaaaacga agacgacgaa gaatcggaag aagatgcgga tgaaatctcg
    20941 gcagccaatc tcgccgaatt gaaacaaaaa gtcatcggcc actttgccca aatcgaaaaa
    21001 gactacaaaa aaatgattgg ccgtttggaa aaacaccaca gccggcacaa agactatctc
    21061 gcctaccgcg acgcgattgc caacaaactt ttggaagtcc gcttcgccac ccggcaaatc
    21121 gacagcctca gcagcagcct gcgcggaaaa gtagaaaaca tccgcaaact cgaacgcgaa
    21181 atccgcgaca tctgcctcga ccgcgtccat atggagcgcg actacttcat ccaaaacttc
    21241 ctgcccgaaa tcaccaatct ggaatggatt gaagaagaaa tcgccaaagg cagggtttgg
    21301 agcgacgcgc tcgaccgctt ccgccacgcc atcctcgaaa aacaaaccga gttggcggat
    21361 atggaaaaag aaacccgcat ttccatcgaa gagttgaaag aaatcaacaa aaatatggtg
    21421 tcaagcgaaa aagaaaccgc agctgccaaa caggaaatga ttcaggcaaa cttgcgcctc
    21481 gtcatttcca tcgccaaaaa atacaccaac cgtggcttgc agttccttga tttgattcag
    21541 gaaggcaaca tcggcctgat gaaagcggtc gataagttcg aataccgcag aggctataag
    21601 ttctccacct acgcaacctg gtggatccgt caggcaatca cccgctcgat tgccgatcaa
    21661 gcgcgtacca tccgcattcc ggtacatatg attgaaacca tcaacaagat gaaccgcatc
    21721 tcgcgccaac accttcaaga aaccggcgaa gaacccgatt ccgccaaact tgccgaactg
    21781 atgcagatgc ccgaagacaa aatccgcaaa atcatgaaaa tcgccaaaga gccgatttcg
    21841 atggaaaccc ccatcggcga cgacgacgat tcgcacttgg gcgacttcat cgaagatgcc
    21901 aacaatgttg cgccggccga tgcggcaatg tacaccagcc tgcacgaagt aaccaaagaa
    21961 atcctcgaaa gcctgacacc gcgtgaggca aaagtcctgc gtatgcgttt cggcatcgat
    22021 atgaacaccg accacacgct ggaagaagtc ggcagacagt ttgacgtaac gcgcgaacgc
    22081 atccgacaaa tcgaggcaaa agcactccgc aaattacgcc atccgacaag aagcgaccgt
    22141 ttgagaagtt tcttggacag cgaagacagc aagctgtaaa ccaaaaaacc gcaggtttca
    22201 aatacctgcg gttttttctt acacaataaa caacgcttcc acatatccca cactcctatc
    22261 ccgagacctt tgcaaaatcc cccaaaatcc cctaaattcc caccaagaca tttaggagat
    22321 tttccatgag caccttcttc cggcaaaccg cccaagccat gattgccaaa ca
//
LOCUS       DAWGJV010000013        22344 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWGJV010000013 DAWGJV010000000
VERSION     DAWGJV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.013"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(120..1034)
                     /locus_tag="WHQ94_000374"
     CDS             complement(120..1034)
                     /locus_tag="WHQ94_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2127713.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1301..2770
                     /locus_tag="WHQ94_000375"
     CDS             1301..2770
                     /locus_tag="WHQ94_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ2127714.1"
                     /translation="MDKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3022..3393
                     /locus_tag="WHQ94_000376"
     CDS             3022..3393
                     /locus_tag="WHQ94_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127715.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3466..4875
                     /locus_tag="WHQ94_000377"
     CDS             3466..4875
                     /locus_tag="WHQ94_000377"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ2127716.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLETLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4922..5716
                     /locus_tag="WHQ94_000378"
     CDS             4922..5716
                     /locus_tag="WHQ94_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127717.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5904..6680
                     /locus_tag="WHQ94_000379"
     CDS             5904..6680
                     /locus_tag="WHQ94_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ2127718.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6756..6862
                     /locus_tag="WHQ94_000380"
                     /pseudo
     CDS             6756..6862
                     /locus_tag="WHQ94_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7441..9912)
                     /locus_tag="WHQ94_000381"
     CDS             complement(7441..9912)
                     /locus_tag="WHQ94_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ2127719.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     TQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9909..11087)
                     /gene="hflX"
                     /locus_tag="WHQ94_000382"
     CDS             complement(9909..11087)
                     /gene="hflX"
                     /locus_tag="WHQ94_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ2127720.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11092..12372)
                     /locus_tag="WHQ94_000383"
     CDS             complement(11092..12372)
                     /locus_tag="WHQ94_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ2127721.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12525..13250)
                     /locus_tag="WHQ94_000384"
     CDS             complement(12525..13250)
                     /locus_tag="WHQ94_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ2127722.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13323..14000)
                     /gene="radC"
                     /locus_tag="WHQ94_000385"
     CDS             complement(13323..14000)
                     /gene="radC"
                     /locus_tag="WHQ94_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ2127723.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVCSF
                     RQLGLMP"
     gene            complement(14123..15472)
                     /gene="gshA"
                     /locus_tag="WHQ94_000386"
     CDS             complement(14123..15472)
                     /gene="gshA"
                     /locus_tag="WHQ94_000386"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ2127724.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15730..17139
                     /gene="leuC"
                     /locus_tag="WHQ94_000387"
     CDS             15730..17139
                     /gene="leuC"
                     /locus_tag="WHQ94_000387"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ2127725.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLITVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17236..17490
                     /locus_tag="WHQ94_000388"
     CDS             17236..17490
                     /locus_tag="WHQ94_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127726.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17552..18193
                     /gene="leuD"
                     /locus_tag="WHQ94_000389"
     CDS             17552..18193
                     /gene="leuD"
                     /locus_tag="WHQ94_000389"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ2127727.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18367..19638
                     /locus_tag="WHQ94_000390"
     CDS             18367..19638
                     /locus_tag="WHQ94_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ2127728.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKTQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19654..19815
                     /locus_tag="WHQ94_000391"
     CDS             19654..19815
                     /locus_tag="WHQ94_000391"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ2127729.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            19816..20385
                     /locus_tag="WHQ94_000392"
     CDS             19816..20385
                     /locus_tag="WHQ94_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ2127730.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFEYLEDFVSAIEETVYQNPATRHNASLWKR
                     KFEEAYKKHYNRSISIPRWHEIAHKYQKK"
     gene            20419..21489
                     /gene="leuB"
                     /locus_tag="WHQ94_000393"
     CDS             20419..21489
                     /gene="leuB"
                     /locus_tag="WHQ94_000393"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ2127731.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVSYEYAPLGG
                     EAYDEYGHPYPEFTRNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21652..22215
                     /locus_tag="WHQ94_000394"
     CDS             21652..22215
                     /locus_tag="WHQ94_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ2127732.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5381 a         5879 c         5796 g         5288 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agtttttcgg atgatgatta ttaaaaaaaa gattggtttt attgcctgtt tgggctttaa
     1201 atggggtgac ggcttccttt cggtggcccg aacgcagccc atatcaaaaa gaaaagtcat
     1261 gcgccccttt tgcgaggcgc gatatataag gaggaagatt atggacaaac ataatgggac
     1321 ttatcgggat ttgcaccgtc cggcttcgga atttgcgacg cgggacgaat atttggaaca
     1381 tgaattgcag attatgcagc caaaacgctg gcggcccaac ctgccctttc gcgattaccg
     1441 cttcgagtgg gaagatttga ttcctgcgat ggcgggaacg attggaaaag tggtgatggt
     1501 gggggcggtg gcggcggcgt ttgccgcacc tttggggctg cctgacagct ttgtactgga
     1561 aaatgtgcgc tatgagcttt taatcgccgc cgcgtttatc ttattggtat cgggcttttt
     1621 tctgcccggc gccaacctgc ccggtacgca cgggccgctg attccgatga ttcccatcgt
     1681 tgtgtcggca ggcgggcatc ctttggcgtt cggcatttcg attgcggttt taggtctgct
     1741 gatggcttta tttcgcggcg gcagtattat ggcgaagctg acaagcaacg gcgtatgcgg
     1801 cggattatta ctctatttgg gctttatcgg cacgacgggg caagtaaaaa aattgttttc
     1861 gtgggcaggc ggttttaata tgccctacat cgcttttacc gtcattattg taacgattgt
     1921 gatgtacgct ttgttggagc attggaaaaa acgctggtta gccgtgcctt tgggatgctt
     1981 gattgccggt gtggtggcat ttgcattggg tgcgccgttt gagtttcaca ccgcccccgg
     2041 cctgcctcca atgagtcctg cttattggtg gggtgaaaac agcggctggc atctggggtt
     2101 gccgacggca gaaagttttt tggttgtctt tccatttgcg gtattggctg ttgcaatgtg
     2161 gtcgcccgat tttttaggac atcaagtgtt ccaaaaaatc agctatccgg aaaaaaccga
     2221 taaggtattg atgaatatag acgacaccat gacaagttgt tctgtccgtc aagcagtggg
     2281 ttctatttta gggggtgcaa attttacctc ttcttgggga acttatatcg taccggcatc
     2341 gattgccaaa cgccccattc cgggcggtgc ggttttaacg gcggttttat gtattatcgc
     2401 cgggttatgg ggctatccga tggacttggc gatttggcag ccggtattga gcgtagcctt
     2461 ggtcgtaggc gtatacttac cgcttttgga agcgggcatg gaaatgacgc gcaaaggcaa
     2521 aaccacccaa tccgccgcca tcgtggtgtt ctcttccgcc ttggtcaatc cggttttcgg
     2581 ctgggcgttg acgatgctgt tggataattt gggcttaatc ggctgcaaag agcgcagtgc
     2641 gcaattaggt ttcgccggac gcgtgttgat acccgcagta ggtttcttga tcttgtgtgt
     2701 ggcgatgggt gcggtcggga tgctgcccgg tatcccgccg tttttggaac acttcaaatc
     2761 tttgggctag gctgaaatcg gaaatgccgt ctgaaccgct ttcagacggc atttttgcaa
     2821 acaggcaaaa tgacggcggc gggatttttt attttcccga ttgaagtata atgttgccgg
     2881 gcttcaaccg gatattcaaa acggtttgtt ccaacactcg gaacggcgca taaaacgccg
     2941 cccttcgcgt tatcccgaac ggggcggcta atcagattct atcgccataa aaggcggggt
     3001 ttcaaccgaa aaggaattga gatgaataaa accttgtcta ttttgccggt ggcaatctta
     3061 ctcggcggct gcgccgccgg aggcggtaac acattcggca gcttagacgg tggcacaggc
     3121 atgggcggca gcatcgtcaa aatggcggtt gggagccaat gccgtgcgga attggacaaa
     3181 cgcagcgaat ggcgtttgac cgcgctggcg atgagtgccg aaaaacaggc ggagtgggaa
     3241 aacaagattt gcgcttgcgt cgcccaagaa gcacccgaac ggatgaccgg caacgatgtg
     3301 atgcagatgc tggctccgtc cacgcgcaat caggcacttg ccgccctgac cgccaaaacg
     3361 gtttccgcct gcttcaaaca cctgtaccgc taaccttctt cagacggcat gaggcagatg
     3421 ccgtctgaag ccgttttcct gcaatttcga cagacgagaa aaatcatgaa atacatcagc
     3481 acgcgcggcg aaaccgcaca caaaccgttc agcgaagttt tattgatggg gcttgcaccc
     3541 gacggcggcc tgatgctgcc ggaacattat ccgcaaatcg ggcgggaagc cttggacaaa
     3601 tggcgcggtt tggcttatcc cgaattggcg tttgaaatca tgcgcttgtt cgttacggat
     3661 attccggagg acgatttgcg cgatattctg aaccgtactt acacggaagc ggcgttcggt
     3721 actaaggaaa tcacccccgt ccgcacgctt tcagacggca tcaaaatcca agccttgtcc
     3781 aacggcccga cgctggcgtt caaagatatg gcgatgcagt ttttgggcaa tgcgtttgaa
     3841 tatgttttaa acaaagaagg taaaaaactc aatatcttgg gcgcgaccag cggcgatacg
     3901 ggttcggctg cggaatatgc cttgcgcggc aaaaaaggcg tgaacgtatt tatgttgtca
     3961 cccgacggta agatgagcgc gttccaacgc gcgcaaatgt acagcctgca agacggaaat
     4021 atccacaata tcgccgtgaa ggggatgttt gacgattgtc aggacattgt gaaggcagtg
     4081 caaaacgatg ccgcgttcaa ggaaaaatac catatcggta cagtcaactc gattaactgg
     4141 ggacgcatcg tcgcgcaagt ggtttattac tttgcgggct atttcaaggc gacgcaaagc
     4201 aatgacgagc aggtcagctt ctgcgtgccg agcggcaact tcggcaacgt ttgcgcggga
     4261 cacatcgcca aacagatggg tttgcctatc cgccgcctga ttgtcgcgac caatgaaaac
     4321 gatgtgttgg acgagttttt caaaaccggt gcataccgcc cgcgcaacag cgcgcatact
     4381 tatgttacct ccagcccgtc gatggacatt tccaaagcgt ccaacttcga gcgtttcgtg
     4441 ttcgacctga tggatcgcga tcctcaggaa atcaatacgc tgtgggcgga agtagcggcg
     4501 ggcaaaggct ttgacctgcg gtttgcttta gaaaaagtcg gtggcaaata cggttttacc
     4561 tccggcaaat ccacccaccc cgaccgcctc gccaccatca gacaggttta tgagcaagac
     4621 caagaactca tcgacccgca cactgccgac ggcgtaaaag tcgcccgcga agtgcgcgaa
     4681 gaaggggaaa cggttgtgtg tttggaaacc gcgttggcag cgaaattcga tgcgaccata
     4741 cgcgaagccg tcggcgatgc cgccattccg cgccccgccg cgctggaagg tttggaaaca
     4801 ttgccgcagc gcgtgcaaac cgtgccgaac agtgcggatg cggtaaaagg catcatcgaa
     4861 caaacccttg cctgatgttc cgtatgggcg gcattccgtc cgaacttaag attgaaagaa
     4921 aatgacctta cctatgcagg aaacccgttt ttccatactg ctggacgaat tggcggcaaa
     4981 acaggaggca gccatcgccc cccacctgct tgccgacggg agcaaggtat ggatacgcaa
     5041 agcaggcagg cacaatgcac gatggcgtta cgcgctgctc ggtatggttg cccgatattt
     5101 gaaactgggg gtgttgaagc cggtccccag cctcggcggc gagcctgcca ttgcaaccga
     5161 atcaaaacgc ctgtacgaat tgcgcgcggc ggggatagcc gttcccgaat tgctcgccgt
     5221 ccggaaaaat gcccttatgt tcggcaattt ggaaggcatc ccgctcgata cgcaaatccg
     5281 ccaagaagcc gaggctggaa agtcggacgc ttggcttgcc ggtttggaag ccattgcacg
     5341 cgtgcataaa aaacggcagt tcctcagtca ggcgtttgcg cgaaatatga tgtcggacgg
     5401 aaaaaacatc agctttttgg attttgaaga cgacccttcc gaagtcctga ccatagcgca
     5461 atgccaagcc cgcgattggc tctgctacat ccattcgacc gcgctgatat tgaaaaacgg
     5521 cggactgctg gaagcggctg cggaaaaatg gggcggcgta ttgtcagatc agcctgccga
     5581 aatacaaaag ctgattgccg ccaccgtcaa accgatcctc ccgatacgca ggctggaaca
     5641 cccccgctgg ggacgggacg cactcaggct ggcagcctcg atttccctga tttcccttgc
     5701 cgatatgccg ccgtaagccg ccgcctgccc cgcgatgccg tctgaagccc ttcagacggc
     5761 atataaccaa cgcgcatcaa gcattgtcgc aaccatccgt ttcgtttaga attttggctt
     5821 tatcccgaat cgggcagaca tttccactct gccgcacctc ccgaacaagc actatccatt
     5881 tcttttactg acaggaaacc caaatggcag cattcaatac ccaaaaagta ttgtccgtac
     5941 accactggac agacgcatat tttaccttta cctgcacccg cgacgaatcg ttgcgctttg
     6001 aaaacggaca gttcgttatg gtcggattga tggtggacgg caagccgctg atgcgtgcat
     6061 acagcgtcgc ctccgccaac tgggaagaac acctcgaatt tttcagcatt aaagtccaag
     6121 acggcccgct gaccagccgc ctgcaacacc tcaaagtcgg cgacgacgtg ttaatcagca
     6181 aaaaaccgac cggaaccctg gttgccggcg acctgaatcc gggcaaacac ctttacttgt
     6241 tgagcaccgg taccggcatc gctcctttct tgagcatcac caaagacccc gaaatttacg
     6301 agcaatttga aaaaatcatc ctcgtacacg gcgtgcgcta caaaaaagat ttggcgtact
     6361 acgaccgctt taccaaagaa ttgcccgaac acgaatacct cggtgacttg gttaaagaaa
     6421 aactgattta ctacccgatt gtttcgcgcg aagaattcga acaccacggt cgcctaaccg
     6481 acctgatggt gagcggcaaa ctgtttgaag acatcggcct gcccaaaatc aacccgcaag
     6541 acgaccgcgc catgctgtgc ggcagccccg cgatgctgaa agatacctgc aaagttttgg
     6601 acgatttcgg actgaccgtc tctccgaaaa caggcgtacg cggcgattac ctgattgagc
     6661 gtgcgtttgt agatcaataa gctgtttgaa aatcccgtct gaacggcacc agcaatcaaa
     6721 aaccccgaat cctgccaatc aggattcggg gtttttatag tgaattaaat ttaaatcagg
     6781 acaaggcggc gagccgcaga cagtacaaat agtacggcaa ggcgagccaa cactgtaccg
     6841 gtttaaattt aattcactat atagtgggta aaaattagaa atgcacattt ccgtcattca
     6901 cgcgcaggcg ggaatccaga cctatccgtt aattagcatt tgaaaataaa agaaaaacca
     6961 agcatatgga ttccccccta cgcgagactg acgacggtgt tctgcagtag ccaaagcaga
     7021 aaaccatcct tattgtcatt tcatcatgat ataacaataa ggatgttaaa aattagttat
     7081 cattatatgc atgatatata agaaaaattg tcccaataag aaatgttatg cagaaaataa
     7141 aatgaaaagg atggttttca ttgaaaatgg ttgttctgcc accgtcatac acagaaccgg
     7201 tatttggaaa aaaaataatc catattgaca tcattattcc atttatcgca taaatccact
     7261 ttttaatttt tttcctcatt aatacaatct cttatttaat aaaatcaaat acctagcaat
     7321 tcgatctcaa attttgaacg ccaacgtgtt tgagcggtaa caatacatac tttaagtttg
     7381 caattcttca tcgtcttatt tttgttttca tcaactatac gtctaaagca acgccgtccg
     7441 tcattgttcg gacggcattt tttcagactg cattttcccc cgtttgtgaa ggcgcagggc
     7501 gttgcccaaa accgccagcg aactgaagct catacccagt gcggctatcc acggttggac
     7561 atagcccaaa acggcaagcg gtacggcaat gatattgtac gcgcccgccc atatcaggtt
     7621 ttgccggata atatggcggg tgcgccgcgc ctgatcgagc aggtgggcga cggtacgcaa
     7681 atcttcgttc aataacacaa tgtccgcgcc gtccctcgca atatccgtcc cgcccgctgc
     7741 ggcggcggat acgtctgcct gcgccaaaac gggcgcgtcg ttgatgccgt cgcctatcat
     7801 cagcactttt ttcccttctt tttgcaaggc tttgacgtat tccagtttgt cctcgggcat
     7861 ggcttgggcg cggtagtgcg cgacacccag ggcgcgtgcg gtttcggcaa cggcggtttc
     7921 gcggtcgccg ctgaggatgt gcagggtcag gtttttgcct gccaactgcc gcaccgcctc
     7981 cgccgcgctg tctttcaagg ggtcggtcag gtagaacacg gcttggaaac cgctttgact
     8041 gccgaggtaa accgcgctgc cgccgccttc tgtttgcggt tctttacctg aaatttcggc
     8101 gacataggat gccctgccca atgcccaaac ctgtgtttcg ccgttgacgg tcagttgcgc
     8161 gcccacgcct tcgccgatgc ggttgaggcg ttgtttaata gcgatgtcgg ggacgctgcc
     8221 gtctgaaatg cggcagttga ggatggcgcg ggcaagggga tgttcggact gttgttctaa
     8281 agcctgcgcc accgcgagaa caaaggcttc gtctgtgcct ctcaacaatg agatacggcg
     8341 gacggcgggt ttgccttggg tcagcgtgcc ggttttgtcg aagatgatgt cggtggtttg
     8401 ggcgagggtt tcgattgcct gctttccgcc gattaaaata ccttcgcgcg ccagcgtacc
     8461 ggtagaagct gccagcgcgg tcggcgtggc aagcgataag gcgcaggggc aggtaatgac
     8521 cagcagggcg acggtaatcc acaatgcggt gtgcgcgtcg gcgtacagcg tccagccgat
     8581 gaagacgggg acggcaagca ggagttcgcc gaatatgaaa gacgaggcgt attgttccgc
     8641 caactcggca gtgcgcggtt tttgcgctaa ggcgcggtcg agcaggcgga cgatgtgcga
     8701 cagtcgcgtg ccaccgccgg tgcggtcggt gcgtataatc agggggcttt gcgtgttgag
     8761 tgtgccggcg gttacttttt cagacggcat tttggcgacg ggcaggctct cgccggtcag
     8821 catagattcg ttgacggcac tgcttccttc cagcaccgtg ccgtcaacgg ggatggtttc
     8881 gcccggtttg accagcacga tatcgcccgc tttcaatttg acgacagctg cctcgcaggt
     8941 ttcctgcgta tcggggtaat cgggcatatg atggcaaaac gcaggaatca gcttcaccag
     9001 cctctcggcg gcatcgcctg ccttacggcg ggcaatgtgt tccataaagc gtccgcccag
     9061 caggaaaaac agcagcatcg cgatggattc gaaatacatc ccttgccccg catttgtcgc
     9121 aaggctgtaa acgccggcga taaaggtcat gatgatggcg acggtaatcg gcgtatccat
     9181 gccgacgcgg cggtttttca agtcgcgcag cgcgccttga taaaacggga cggcgcaata
     9241 gaatacgacg ggcagcacca ttaaaaagcc gccccaatgg aggatttgca ggaaatcggg
     9301 ttcgatgtcg ccgccgtaaa ggtaggtcgg cagcgcgaac atcatcgtct gcatcatccc
     9361 cagcccggca acggcgaggc ggacgatgta ttgtttgcgt tctttttggt tggcggcttc
     9421 gattttttgc gtgtcatagg gtgcggcggt gtagcctatc tgcctgattt tcaacagaat
     9481 gtcggaaagg cggattttgc cgtcgtccca gacgacacgg cagcggtgcg tgctgtaatt
     9541 gaggtcgatg cggacgatgc cgtctgtacg caaaagctgc tgttcgatca gccagacgca
     9601 ggcggcgcag gtgatgccgc cgagcattaa aaccgcctcg cgcgtgccgc cgtgggtttc
     9661 cacaaagtcg gactggactt cgggcaggtc gtacaggcgg atttggtcga ggatttcttg
     9721 gggcggcagc tcggtttttt gcgcgtcggc ggtgcgttgt ttgtaataac tgcccaagcc
     9781 cgcgtcaata atgctttgtg cgaccgcctg acagccggcg cagcaggttt cgcggtcttc
     9841 gttttcgtaa cggacagtca ggtggaggtg ttcgggaaca tccagaccgc agtggaaaca
     9901 ggtttttttc atggcatttc ggtttcgtct gtgtttggtg cggcggcaca agactcggca
     9961 atggcttcgc gcagtgcgtc tataccggta ttttcagcaa cggaaatgcg gacggcggca
    10021 atttttcccg cagcgtcgcg ccatatgccc gtgttttgtt cttcagacgg cagcaggtcg
    10081 gttttgttgt acaccttgat gcacggaata tcgccggcat ggatttcttg cagtacgttt
    10141 tccacgtctt caatctgctg tccgctgttc ggagcggcgg catcgacgac gtgcagcagc
    10201 acatcggctt gcgcggtttc ttccagcgtg gcggaaaagg cggaaatcag tttgtgcggc
    10261 agatcgctga cgaatccgac ggtatcggtc aggataatgc tgcattcggg actgatgtac
    10321 agccgccgcg ccgtcgtgtc gagtgtggcg aaaagctggt ctttcgcata tatgcccgac
    10381 ttggtcagcc ggttgaacag gctggatttg ccgacattgg tatagccgac cagcgcaaac
    10441 gttttgattg tgcccgattc gcgggacttg cggcgcaggg cgcgctgttt tttgaggttg
    10501 gcaagctgtt ttttcaaggc attgatccga tgggcgatca atcggcggtc ggtttccagt
    10561 ttggtttcgc cggggccttt catgccgata ccgccgcgct ggctctgcag atggccgtaa
    10621 ccgcgtatca agcgtcccgc caaatggctc aattgcgcca actcgacttg cagcctgcct
    10681 tcctgcgtgc gggcgcggcg agcgaaaatc gccagaatca gccctaccct gtccaatacg
    10741 cggcatttca gttctttttc aaggttgcgt tcctgcgtgg gcgtgagttc gtggttgaat
    10801 acgaccaaat cgatgccgtc tgcggcaact gcttctgaca gctccgccgc cttgcccgtg
    10861 ccgacaaaca gcgcggtgtg cggacggtcg cgtttggcag tctccacgcg cacggaatcg
    10921 ccgcccgccg ctttgaccag ctcgacagct tccgccaatg ccgtctgaaa accgttcaga
    10981 cgggcggcac tactgcccgt accatctttg tccaacatta cgcccaccag catgacgcgt
    11041 tcgggttgcg cttgggtact gccgttgccg cctgttctgc ctgtcatgta ttcaatcctg
    11101 taaataaagg gttacgcctc gttccgtttc tccttccggt gcaagcgtga tgcggctgcc
    11161 cgccattccg tcaacgctga agacgatgcc gcccgcctcg tgccttccta tggacaggcg
    11221 gctgccgggt cgctgccggt aggtataacg ggtacacgct tccaaacacc agcgtccgcg
    11281 ccagtggatg aaatattttt cgccttttct aaccgcaagt gtctcgccgc cgtttacctc
    11341 aaaataaggg taatcgggtt ttcgggttct aaactgccat ttcgcctgcg ctcgccgtcc
    11401 gttgcggaca tagtcgaata ccgccgtata aagcgtgccg tattccaaag gcttgagcgg
    11461 aaaaagcgcg aattggtacg cggtcagcct gccgttgggg tcgttgccgg cggttaaaac
    11521 cctgacgggg cggatttcgt ttttaccctg atacagcttg aaacttttca tcgtaatttt
    11581 gcctgccgcc tcggaaaaat caatgctggc aggattgccc gtgatttcat attccggcac
    11641 ggggtctgga cgctcgccgt ggaaataagg cagtgcgccg ctgccgacgg gataggctgt
    11701 atagagcagc tcctgtgcgg gcatggcttc gtcggtgtac acgaccgcac cgttatggca
    11761 ggcgttgcgg taatatttcc gtcctgcttc cggctgattt ctgccttggg cgcaatgccg
    11821 ctcaaacctg ccgttgccct gattgaatac gagaacggtt ttaccgtttt cgcgcacaaa
    11881 tgccgctcct gcctcatccg tatggcggtc aagtagggaa aggcggtggt agattgcgct
    11941 cattaatccg tccacttggc gttgctgcgt gcggatgtcg ctgtcggacg attcggcggc
    12001 ttcttcttcc gtgctgatgt tttcatgcac gccgttgtag agatacccgg caaggcgtgt
    12061 gcgttcggtc agcttttgtg cggtgtagtg cggattgtcg ggatggtgtt cgccgtgtcc
    12121 gtcttcggga ttgagcgtga ggtagcttgc gtgcctgcgg gcggagtttt ccaaaaccgg
    12181 cgcgtgtgcc agcttgtgca aaccgatttg ggctcggatg cggttgaggt agtgcagcgc
    12241 atcgccgccg tccctgattt gcggtgcgga cggatagaca agttcgcccg cgggcaggga
    12301 ttggttttgg gtatggtaga aaacgcctgc cgccgaaccc aacaggagca gccgaataaa
    12361 aagggatttc ataatgccgt cctcccgacg cggcaacggg gtgttttatc aaaacggcaa
    12421 acttgccaat gcggtttccg attgcagatt gccaaaccgg caaacctttc cccgtgccga
    12481 tgccgaaccc gatgccgtct gaaccttcag acggcatcgg gtgtttattt gtccacccgg
    12541 atgggggcag gttcgtattg tgtcgattcg tcgccgtaat acagcacgcc gagtttgatg
    12601 gggattctgc cctgtgattt gcggtgggca ttggaatccc tcagggaata ggcacaaccg
    12661 caatattcct gctggtagaa gttttcacgt ttgctgattt caatcatgcg cgcgctgccg
    12721 ccgcctttgc gccagttgaa atcccaatac accacatcat cgtaaggcgc ggcggcgcgg
    12781 tgtccgcagt cgttgatttg cgccatattt ttccagcgtg aaatgcccag cgaactggta
    12841 aagacgggga acccgtgttc atgcgcgtat tgcgccgcct tttcaaaacg catatcgaaa
    12901 cacatggtgc agcggatgcc gcgttccggc tcaaactcca tgcctttggc tttggcaaac
    12961 cattctttgc ggtcgttttc gtagtcgtcg tctttatcga tgaaaggaat gccgaacttt
    13021 tccgcaaagc gcacgttttc ctcttttcgg agcatatact ctttgtgcgg atggatattg
    13081 ggattgtaaa aataaatggt gtagtcgatg ccgctggcca gcatggcttc catcacttcg
    13141 ccgctgcacg gggcgcagca ggaatgcagc aagacttttt tatgtccacc gggcgggacg
    13201 agtatagggc ggtcaatgtc ggtaacggta ggtttgtttt gtgtttccat taacttaaca
    13261 atatctgttg aatacggttg aagcggctat tttatcagag ccgccgcatg taaaacagag
    13321 tgtcagggca tcaacccgag ctgtctgaac gaacagacgc tttgcgaggt aacgataaaa
    13381 tggtcgagca gcgacacatc gaccagcgac attgcctgtg ccagccgcct tgtgaacatg
    13441 atgtcttcct gcgaaggttc gggcgagccg cccggatggt tgtgcgcaat aatcaggctg
    13501 tcggcatatt cgtccaatgc cagtttgacg atttcgcgga tgtaaatcgt gttttccgca
    13561 accgtaccgc gcgacagctc tctgaccgca atcagttggt tttggcggtt cagcagcagc
    13621 gcgacgctga cttcgacttt ttcctgcccc aaatgaaagc gtaaataatc ggccaccgta
    13681 tccggatcgg acaggacgat gctctcctgc aattcttccg ccaatatccg ccgcccgatt
    13741 tccctgacta cggcaaactg tgtgaaactt gccgtcccca tccctttgta tgccgacagt
    13801 ttgccgacct ccgcgctcat cagcctcccc aaactgccga actcctgcag caaataacgc
    13861 gccaaatcaa ccgcactcat tccgcgcgtg ccgacgcgca gcaggattgc caaaagttcg
    13921 gcatcactca aagccgccgc cccgcgttcc aacagctttt ccctgggcct ttccccctcc
    13981 ggccattgct tgatgctcat gttttccctt ttttaaatag tctgagatgc cgtctggaac
    14041 aaatgttccc acacgccaaa tatccgaaat gccgtctgaa ccggatttcc gcttcagacg
    14101 gcatctgtat ttcaaaacag tcttacgccg tttcttccaa ctcgatggac gccgccagaa
    14161 gcgacaggcg tgccattacg ccgtaaacgt agaggcggtt gtgttcgttg tccacgtcgc
    14221 agtctttttc agagcgcggc atacccagcg agtcccattg ttcgaatacg cgcttgcagg
    14281 cttcgggcgc ttcttgggag ttgtcgccgt tgccggtggg gatgctgttg gacagcggaa
    14341 caaacaccat accgccggcg ttgaggtttt cgtccgcacc gcgcccttcg tgtacgcgga
    14401 aaaagccgcc gatgacgaag cggtccatca tatacacgac gggttcgcac accgcgccgt
    14461 ttaaggtttc ataagtataa atcccttctt ggacaatcac ttcgctgact tccaagcctt
    14521 ctttgacttt cgccatttta ttgcggtttt tacggttcaa tccgcgcact tcgtcggcgg
    14581 atttgacgct catcacgccc atgccgtaag tgccggcatc ggctttgacg atgacgaaag
    14641 gtttgtcggt aatgcccgat tcgtcgtatt tggcttgaat tttcgccagc acacgttcta
    14701 ccgcttccgc caacgcgtct tcgccttcac gcccttggaa gtccaaaccg ccgatttttt
    14761 caaaataagg gttaatttgc cattcgtcga tgccgattaa cttggcaaat tcggcggtaa
    14821 cttggttgta cgcgccgaaa tgatttgttt tgcggcgcgt cgtccaaccg ccgtgcaacg
    14881 gcggcaaaac ggtttggctg atgcctttga ggatgtcggg aatgccggcg gacaaatcgt
    14941 tgttcaacaa aaccacgcaa ggcgaaaagc cgtctgcaag atggacgcgg tcgcgggtac
    15001 gcaataaagg ttccaacagg attttgtcgc ccaatgcggt ttcaaattcg gtcggttcgg
    15061 ttacttccgg attcaggctg cccaagcgca cttcataccc tgccgaacac aaaatctcgc
    15121 cgagggcgta aacgttttgc aggtaaaacg tattgcgcgt gtggttttcg ggaataatca
    15181 atacggattt cgccgtttca caggcgcgtt gcaccgcatc ttgcgcggca accgccgcca
    15241 gcgggataaa gttgggattc agattattga agccgccggg gaacaaattc atatcgatag
    15301 acgaaatttt gtaaccggca ttgcgtatat cgaccgaacc gtaaaacggc gggcggtgta
    15361 cgctccactg cgtgcggaac cacgcctcga ttttggcgtg attggacagg atttttgcct
    15421 caaacgcctg aagttgcgcc gaatgttcgg gcgacatcac cggtaatttc atctttcatc
    15481 tgcctctgtg tgttttttga caagatgcag atgataatgc ctttgaaaga atgacgacaa
    15541 gtattctttc gtatcgaatc atgctttaga tataactgtt tactgaaatg gacaatatgt
    15601 ctaattttta tctataaatt gttttcgatt ttctatttct tcaaaaatta gacaaatttt
    15661 gttgcatggc gtgtaaaatc cgtctagaat cttcatccta cattactacc cacataaaag
    15721 aacaacacca tgaccgcaca aaccctctac gacaaactct ggaacagcca cgtcgtccgc
    15781 gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc
    15841 agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc
    15901 gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac
    15961 ccgatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc
    16021 gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg cccggaacaa
    16081 ggcgctaccc tgcccggcat gaccgttgtc tgcggcgact cgcacacttc tacccacggt
    16141 gcattcggtg cgttggcaca cggcatcggc acttccgaag tcgagcacac catggcgacc
    16201 caatgtatta ccgcgaaaaa atccaaatcc atgctgatta ccgttgacgg caaattaaaa
    16261 gcgggcgtta ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcgggc
    16321 ggcacgggct acgccatcga atttggcggc gaagccatcc gcagcctttc tatggaaggc
    16381 cgcatgactt tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc
    16441 gaccaaacca ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg
    16501 gacaaagccg tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa
    16561 gaataccgtt tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa
    16621 atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa
    16681 cgcagcggta tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac
    16741 gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg
    16801 cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta
    16861 atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt
    16921 ttcatcgaag ccggttttga atggcgcgaa ccgggttgct caatgtgcct tgcgatgaac
    16981 gccgaccgcc tcgcaccggg gcaacgctgc gcctccacct ccaaccgcaa ttttgaaggc
    17041 cgtcaaggca acggcggacg tacccacctc gtcagccccg ctatggcagc cgccgccgcc
    17101 gttaccggcc gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa
    17161 gaatttgacg cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa
    17221 ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc
    17281 gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca
    17341 caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac
    17401 aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc
    17461 ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc
    17521 agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat
    17581 cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt
    17641 aaaatccatc aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga
    17701 ccacggcgaa ccaggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa
    17761 ccagccgcgt taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc
    17821 ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc
    17881 cagcttcgcc gacatcttct ttaacaactg ctacaaaaac ggccttttac ctatcgtgtt
    17941 gaccgaagaa cgggttgacc gacttttcaa agaagttgaa gccaacgaag gctatcagct
    18001 ctccatcgac cttgccgaac aaaccctgac cacaccaagc ggcgaaacgt tcacatttga
    18061 cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg gtctgaccct
    18121 gcaacacgcc gacgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct
    18181 gtttaacggt taagcctatg ccgtctgaag gccgttcaga cggcattcct gataacatat
    18241 aattgttgtg gctaatgtaa acagagggat tatcaattgc tttcatcttt taagcgggct
    18301 tagcaacact ctaaattaat gcaataatgc aactctccat ttcaaataaa tttttagatt
    18361 tttaatatgc aacaaattaa atttattgac ttattttccg gaatgagcgg tatcaggaaa
    18421 ggattcgaac aagcctgtcg gaaacagtcg gttgcttgcg aatgtgtttt tacttcagaa
    18481 atcaaaccgg cagctttgga agtattgaag caaaactacc ctgatgaagt gccgtatgga
    18541 gacataacga aaattgaaac gggagatatt cccgattttg acatcttgtt ggcaggcttc
    18601 ccttgccagg ctttttcttt cgccggaaaa agattgggct ttgaagatac gcggggaacg
    18661 cttttctttg atgtggcaag gattttaaaa gcaaaaaaac caaaaggttt tattttggaa
    18721 aatgtggaag gattggtgac acacgataga aaagatccga cacaaaaaat agggcgcacc
    18781 ctgaccgtta ttttggaaac cttggaagca ttgggctact atgtttcttg gaaagtttta
    18841 aatgcaaaag atttcggcat tccccaaaac aggaagcgca tttatctgac aggcagtttg
    18901 aaatccaaac ctgacttatc tttcgaaaca agcccaagtc cgaaattaaa aaatattttg
    18961 gaatcgggac tgcctaccga aagcagccct ttcatcaaaa aattgctaaa aaaatttccc
    19021 ccgtccgaac tgtacggaaa atcagtaaaa gacaaacggg gagggaaaaa caatatccac
    19081 agttgggata ttgaattaaa aggcacggta accgaagaag agaagcaatt gttaaatatc
    19141 cttctaaaag aacggaggaa gaaaaaatgg gcttcagaaa tcggcatcga ttggatggat
    19201 gggatgcctt tgacaaaaac acaaatttca actttctata aacaccccga tctccaaaat
    19261 attttggaca gcctgacaga taaaggctat ttggttttag agcatccgaa acaaaaaatt
    19321 ggcgggcaaa gaatcaaaga tgaatccctg cccaaaggct acaatattgt ttcaggtaaa
    19381 aaatcatttg aaatcaataa aatattagat ccaaacgatg ttgcgccaac cttagttgca
    19441 atggatatgg agcacctttt cgtcgttgac aacggcggtt tgcgtacact taccggaaaa
    19501 gaagggttgc gcttattcgg ctatccggac gattattcgt ttgatattcc caaaaaagac
    19561 agatgcgatt tattgggtaa taccgttgcc gtccctgtga ttaaggcggt atctgaaaga
    19621 cttctgcata ctttataaac cgacagggat aaaatgataa aacttactgc acaacaaata
    19681 tttgataagt tgctggatga agaaaaaatc ttatcggcca atggtcaaat tagatttttc
    19741 ttaggcgatg tggatattat cgtcaaacaa aaagatgttg tcggcaacat cattcaggaa
    19801 tggctcggcg gatgattgag aaaaagggaa attgaatttg atgtttcaac caatacccaa
    19861 atgcctcccg atttcttttt aaataagaaa gaccgcagca gagaattgtt ggaagtaaag
    19921 gcgttcaacc gaaatgccgg cccgggtttt gatattgcag attttaaaat gtattctgat
    19981 gaaatcattc ataagcccta tatgctggat gtagactatt taatattcgg ttacgatatg
    20041 gacgacaacg gcaatgtaac catcaaggat ttatggctta aaaaagtatg gcaaattacg
    20101 agaagcatgg atgggtgggc aattaatctt caagtcaaaa aaggcgtggt gcataaaatc
    20161 cgcccgggtg tttggtacag cataaataaa aagaatatgc ctatgtttga atacttggaa
    20221 gattttgttt ccgcaattga agaaaccgtt tatcaaaacc cggcaacacg gcataacgca
    20281 tccttatgga aaaggaaatt tgaggaagcc tataaaaaac actacaaccg atcgatttcc
    20341 ataccccgtt ggcacgaaat tgcacataaa taccaaaaga aataaccctt caaccatcaa
    20401 cccataagga aacttaaaat gaccaaacat atcgccatcc tccgaggcga cggcatcggc
    20461 cctgaaatcg tcgccgaaac cgtccgcgta ctcgacaaac ttatcgccca aggtttggat
    20521 gtcagctacg aatacgcgcc tttgggcggc gaagcctatg acgaatacgg ccatccctat
    20581 ccggaattca cgcgaaacct ctgccgcaaa gccgatgccg tcctgcttgg tgcagtcggt
    20641 tctccgcaat acgacaatct cgaccgccca ctgcgacccg aacgcggctt gttggcgatt
    20701 cgtaaggatt taaacctgtt tgccaattta cgccccgcca tcctctaccc cgagcttgcc
    20761 aacgcctcca cgctcaagcc tgaaatcgtt gccggtttgg atattttgat tgtacgcgaa
    20821 ctcaccggcg atatttattt cggcgaaccg cgcggcatcc gcgttctgga aaacggcgaa
    20881 cgcgaaggct acaacaccat gaaatacagc gaaagcgaaa tccgccgtat tgcccacgtc
    20941 gccttccaat ccgcccaaaa acgcagcaaa aaagtctgct ccgtcggcaa agccaacgtt
    21001 ttggaaacca ccgaactgtg gcgcgaaatt tttgaagaaa tcggcaaaca ataccctgat
    21061 gtcgagcttt cccatatgta tgtggacaat gccgctatgc agttggtacg cgcgcccaaa
    21121 caattcgacg tcattgccac cggcaacatc ttcggcgata ttttatcgga cgaggcctct
    21181 atgctgaccg gctccatcgg tatgctgcct tctgcttctt tggacgaaaa cggcaaaggc
    21241 ctgtacgaac cgtctcacgg ctccgctccc gacatcgccg gacaaaacaa agccaacccg
    21301 ctggccacca tcctctcgct cgcaatgttg ctgcgttaca gcctgaacga cgaagcgcgc
    21361 gcgcaacaag ttgaaaacgc cgtccaaaaa gtgctgcaac aaggcttgcg taccagcgac
    21421 atttacgaag aaggcacaaa actcgtttcc tgctccgaaa tgggcgatgc ggtactcgcc
    21481 gccttgtaaa aaaacaaagc gcggatcaca ccgcgctttt tcatctgccc ttttccgctc
    21541 gccccgttcg tcaaccgcct gcaaacccac cgtttaccgg caggaaatgc cataaatccc
    21601 tgcctatgtt aggataaacg cctttaccgg cccacacaaa ggattatcat catgaaaaaa
    21661 atcatcttcg ccgcactcgc agccgccgcc atcagtactg cctccgccgc cacctacaaa
    21721 gtggacgaat atcacgccaa cgcccgtttc gccatcgacc atttcaacac cagcaccaac
    21781 gtcggcggtt tttacggtct gaccggttcc gtcgagttcg accaagcaaa acgcgacggt
    21841 aaaatcgaca tcaccatccc cattgccaac ctgcaaagcg gttcgcaaca ctttaccgac
    21901 cacctgaaat cagccgacat cttcgatgcc gcccaatatc cggacatccg ctttgtttcc
    21961 accaaattca acttcaacgg caaaaaactg gtttccgttg acggcaacct gaccatgcac
    22021 ggcaaaaccg cccccgtcaa actcaaagcc gaaaaattca actgctacca aagcccgatg
    22081 gcgaaaaccg aagtttgcgg cggcgacttc agcaccacca tcgaccgcac caaatggggc
    22141 gtggactacc tcgttaacgt tggtatgacc aaaagcgtcc gcatcgacat ccaaatcgag
    22201 gcagccaaac aataaatctt gatgcgaatc agcacaaatg ccgtcttcat caaaccgccc
    22261 cgccaacggt aagccgatat ggcggagcat aatccgccga aagatatttc agcggcaggc
    22321 agggcttgcc ggaaagtccg gaaa
//
LOCUS       DAWGJV010000014        21680 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWGJV010000014 DAWGJV010000000
VERSION     DAWGJV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.014"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WHQ94_000395"
     CDS             complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WHQ94_000395"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ2127733.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2852..4057)
                     /locus_tag="WHQ94_000396"
     CDS             complement(2852..4057)
                     /locus_tag="WHQ94_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HEZ2127734.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            complement(4083..4805)
                     /locus_tag="WHQ94_000397"
     CDS             complement(4083..4805)
                     /locus_tag="WHQ94_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEZ2127735.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQEIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINTVLLRRSVADRLTQVVSLLPEMYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            complement(5217..6371)
                     /gene="prpC"
                     /locus_tag="WHQ94_000398"
     CDS             complement(5217..6371)
                     /gene="prpC"
                     /locus_tag="WHQ94_000398"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ2127736.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPEREGHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     EEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEDR"
     gene            complement(6457..7332)
                     /gene="prpB"
                     /locus_tag="WHQ94_000399"
     CDS             complement(6457..7332)
                     /gene="prpB"
                     /locus_tag="WHQ94_000399"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ2127737.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(7957..9267)
                     /locus_tag="WHQ94_000400"
     CDS             complement(7957..9267)
                     /locus_tag="WHQ94_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ2127738.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(9406..10584)
                     /gene="ftsZ"
                     /locus_tag="WHQ94_000401"
     CDS             complement(9406..10584)
                     /gene="ftsZ"
                     /locus_tag="WHQ94_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ2127739.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(10703..11947)
                     /gene="ftsA"
                     /locus_tag="WHQ94_000402"
     CDS             complement(10703..11947)
                     /gene="ftsA"
                     /locus_tag="WHQ94_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ2127740.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLQRWIENSF"
     gene            complement(12033..12761)
                     /locus_tag="WHQ94_000403"
     CDS             complement(12033..12761)
                     /locus_tag="WHQ94_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ2127741.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(12751..13665)
                     /locus_tag="WHQ94_000404"
     CDS             complement(12751..13665)
                     /locus_tag="WHQ94_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ2127742.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(13779..15188)
                     /locus_tag="WHQ94_000405"
     CDS             complement(13779..15188)
                     /locus_tag="WHQ94_000405"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ2127743.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(15273..15452)
                     /locus_tag="WHQ94_000406"
     CDS             complement(15273..15452)
                     /locus_tag="WHQ94_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127744.1"
                     /translation="MPSENHETGFRRPYILFFLVFRYMKSSLKMGRKAGFAAGCSLIF
                     NYIAIDMKSCMPKHL"
     gene            complement(15478..16545)
                     /gene="murG"
                     /locus_tag="WHQ94_000407"
     CDS             complement(15478..16545)
                     /gene="murG"
                     /locus_tag="WHQ94_000407"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ2127745.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIECTA"
     gene            complement(16549..17874)
                     /locus_tag="WHQ94_000408"
     CDS             complement(16549..17874)
                     /locus_tag="WHQ94_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ2127746.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(18000..19337)
                     /locus_tag="WHQ94_000409"
     CDS             complement(18000..19337)
                     /locus_tag="WHQ94_000409"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ2127747.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNVDDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCSLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(19422..20450)
                     /locus_tag="WHQ94_000410"
     CDS             complement(19422..20450)
                     /locus_tag="WHQ94_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ2127748.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGDAAAQ
                     YNLGAMYYKGRGVRQDDTEAVRWYRQAAAQGFAPAQALLGSMYAIGQGVRQDDAEAVK
                     WYRQAAEQGDAQAQVLLGVMYDKGEGVRQDDAQAMQRFRKAAEQGDAAAQHNLGLMYL
                     TGEGVRQDYAEAMQWFRKAAEQGIAEAQYNLGVMYHKGAGVRQDYAQAVQWFRKAAER
                     GKAEAQHNLGLMYLTGEGVRRDSKQAAQWFRKAAEQGIVQAQHNLGIRYYKGEGVRRD
                     YKRAAQWYRRAAEQGDAVAQHNLGLMYLKGEGVRQDRALAQEWLGKACQNGNQDGCDN
                     DQRLKAGY"
     gene            complement(20574..21656)
                     /locus_tag="WHQ94_000411"
     CDS             complement(20574..21656)
                     /locus_tag="WHQ94_000411"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ2127749.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIVLPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         5313 a         5928 c         5250 g         5189 t
ORIGIN      
        1 aaggtttcct caattccact ttctggattc ccgcctgcgc gggaatgacg atttacagta
       61 gatgcctgcc atatcgagaa ttacgtgaat gagcaaaatg ttgaacccga cccacgctac
      121 gtcttgcttt tcagacgacc tctaagccgc gttcccttcc aaaaaatcct gtgcaaaccg
      181 ttgcaacacg ccgccggctt catataccaa tacttcttct gcagtatcga ggcggcaggt
      241 aacgggaact tcaacggttt cgccgttttt acggtgaatc acgagggtca ggtcgcagcg
      301 cggtgtacgt tcgccgacca cgtcgtaggt ttccgtaccg tccagttgca gggtatggcg
      361 gttggtaccc ggtttgaact gcaacggcaa cacgcccata ccgatcaagt tggtgcggtg
      421 gatacgctcg aagccttcgg caacaatcgc ttccacgccg gcgaggcgta cgccttttgc
      481 agcccagtcg cggcttgagc cttgaccgta gtccgcaccg gcaatgatga tgagcggctg
      541 tttgcggttc atataggttt cgatggcttc ccacatgcgc atggtttcgc cttcgggttc
      601 gacgcgagcg aacgagcctt ggcgcacgct gccgtcttcg tttttcacca tttcgttaaa
      661 cagtttcgga ttggcgaagg tagcgcgttg ggcggtcaag tggtcgccgc ggtgggttgc
      721 gtaagagttg aagtcttctt caggcaaacc cattttcgcc aaatactcgc ctgcggcact
      781 gacggccaaa atcgcattgg acggcgagag gtggtcggtg gtgatgttgt cgggcaaaat
      841 cgccagcgga cgcatacctc ttaatgtgcg ttcccctgcc agcgcgcctt cccagtaagg
      901 cggacggcgg atgtaggtgg acatcggacg ccaatcgtac agcggactgg gtgctttttg
      961 cgctgtgccg gtgtcgaaca tcggtacata cacatcgcgg aactgctgcg gtttcacata
     1021 ttcggcaacg acggcatcga tttcttcatc ggcaggccaa atgtctttca ggcggatttc
     1081 cttgccgtct gcaacgccga gtacgtcgtt ttcaatatcg aaacggatac tgcctgccag
     1141 cgcgtaggca acgaccaacg gaggcgaagc gaggaaagcc tgtttcgcat acgggtggat
     1201 acggccgtcg aagttgcggt tgcctgataa tacggcggtg gcgtacaaat cgcggtcgat
     1261 gatttctttt tggattttcg gatccagcgc accgctcatg ccgttacagg tggtacatgc
     1321 gaaggcaacg ataccgaagc cgagtttttc catttcaggc agtaggcctg cttctttcaa
     1381 atagatttcg gctacttttg aacccggggc aaacgaagat ttcacccaag gtttacgttg
     1441 caagccgagg cggttggcat tgcgtgccaa cagcgcggcg gcgacaacgt tgcgcggatt
     1501 ggaagtattg gtacaggaag taatcgcggc aataatcact gcaccgtcag gcatttggcc
     1561 gtctgaaggc tcttcataag gtttagccaa gcctttgccg gccaaatcgg cagtcgcaaa
     1621 acgggcatgc gggttacttg ggcctgccat gttgcgcgtt acgctgctta aatcaaattt
     1681 caatacgcga ggataaacgg cggttttcaa ggcatctgcc cacaagcctg cggttttggc
     1741 gtaggtttcc accaatttca cttgcgcgtc gtcgcggcct gtcagtttca aataatcaat
     1801 ggtttgctca tcaatagcga acatcgcggc agtcgcgccg aactcagggg tcatgttgga
     1861 aatagtggct cggtcgccga tagacaggct tcttgcgccc tcgccgaaga attcgacaaa
     1921 tgcgccaacc acgcgctctt tacgcaagaa ttcggtcagt gccaacacga tgtcggtggc
     1981 agtaatgcca ggttgacgtt tgccagtcaa ctcaacgccg acaatatcag gcagacgcat
     2041 catggatgca cggcctaaca ttacggtttc ggcttccaaa ccgcccacgc ctacggaaat
     2101 cacgcccagc gcatcgacgt gtggcgtgtg cgaatccgtg ccgacgcagg tatcggggaa
     2161 agccacgccg tttttgactt ggacgacggg cgacattttt tccagattga tttggtgcat
     2221 aatgccgttg cccgccggaa tcacgtccac attttcaaat gcggtttttg tccagttgat
     2281 gaagtggaaa cggtcttcgt tgcgccggtc ttcgatttcg cggtttttgc ggaaggcatc
     2341 aggatcgtaa ccgccgcact ccaccgccag agagtggtcg acgataagct gggtttgcac
     2401 caccggattc actttggcag gatcgccgcc tttttcggca atcgcatcgc gcaagcccgc
     2461 caaatccacc agcgcggtct gccccagaat atcgtggcac accacccgcg cgggatacca
     2521 cggaaagtcg atttcctgct tcccttctat caactggccc agccagcttt gcagcgtcgg
     2581 caggtcgact ttgtctgcgc ggttgaccaa gttctcagcc aagatacggc ttgtataagg
     2641 cagcttgtcg taagagccgg gtttgatgtc ctcacacgcc gcacgcgcgt cgtagtattc
     2701 caaatccgta ccgggtagcg gtttgcggta acgttggttg gcagccatgt tcagttctcc
     2761 tgtggatcta tttttcttgt ggtttggggt ttcagacgac gttttttgaa gggggtcgtc
     2821 tgaaaaggtt catggattgt tagattagtc ttcagctcaa tagggcatat acaaacgaac
     2881 ctatatagtt accgataatg tagcctacag taccaattaa caaaattgca ccgactaaat
     2941 cacgccaacc tttactgatg gctaaggctg ctgcagtagt cggccctcca atattggcat
     3001 tacaagccaa tacaatttct tcaatactga atttgaacag cttgcctaac actaacgtca
     3061 gtaacaaatt caataccgca ataatcaaag taaaaacaaa gagtaatggt gctgttttaa
     3121 taattaacgg tatagaggca ggaacaccaa tcacaacaaa aaacagataa atacaatatg
     3181 tccctaattc ttgactgcca tctagttttt gaatgccttt acggaaaaat aaaacaactg
     3241 ccaatgtaaa tgtcgttaac agcaaatatt tgtcactaat taaacctgtc agcattttta
     3301 atgcaacacc atcaacactt tgaaaatgat tttgcaaaaa actggataat gagaatgaaa
     3361 ctgcgaccaa caaaatcgag gcagccaaag ataaggctat actctgtagc gaaatggatt
     3421 taggtttcca atactcggca gccttattca cactcggatc atgatattcc tgctccaatt
     3481 tatcctgata tggacttccc caaacttttc tgacccaata tgaggaagag ataccaatca
     3541 aaataaaaaa gtaacaagcc atcatcaaat tatcagcaac aattgttgct gcaataacat
     3601 ctttagcagg gttaagctta gctgacatag ctgcaaaatt aacaccacct cccgtataag
     3661 atgctgaaat catgccagct accttatcta gttcaggaat atagtttttc aatagtgcaa
     3721 atgagacaag cgttccaatg accgtcccta gtgaactaat caggaaaata aacagcatac
     3781 gtccactttc cttaaataga gcatgaagat ctaattgaaa caataacaac ggaatagcaa
     3841 gaggtacaat ataagaccat acagcatcaa atactggagc atccgtcgga ataacaccca
     3901 aattagaagc aatcaacgca atcaataaag ctaatactgc accagggact ttcccagcca
     3961 agcgactgtt ttgctcaata aaaatcgcag cagcggcagc taacataaca aaagcccaca
     4021 gcagcaaatg attatctgct gaaattaaag tttgcatttt tcctcccaat ttttatttta
     4081 atttaagtat cctgctcttc taccgcccca aaaactgcgt tgctttgttg ggtaaaataa
     4141 gcccaatttt ggttgccata atcgaaatgc caccattcgg ttggcaaatt actaaatcct
     4201 gcctctttca tcgcatgaat caaaatccgg cgtaaattac gagcagagga gaaatactca
     4261 ccttctttct ctaaagcatc gctataagat aaatgtccgg ttgcatcaaa ctcacttccc
     4321 atatccaaac ttttgccact ttttatatca aacaatccga catcaacaga tccgcctgtc
     4381 aaatggggca gaggcattcc tgcccgatct ggatcagcag caaaacagtc cagtgcttct
     4441 ctaacttgaa cttcatcata ttccggatgg cgtttttcaa tttcttgctt catattttct
     4501 cgtaatgctc tttgcactat gacaggacgc caaccatcaa aaatttccaa tccgtacatt
     4561 tctggcagca aagagacaac ctgagtcagc ctatcagcaa cacttctacg caataaaaca
     4621 gtatttattg caccttcaat accctgggaa aaataaacag gattgacacg aatgcgtccg
     4681 gtattaccaa cactgaccaa cggttcccca cattcagaaa taggtatttc ttgtattttc
     4741 tgccaaacta actctttggt attttgtacg ggaataccag cataagaccc cttgtcctta
     4801 tccatcaaat gttccgcatt aataattagt atgccaataa attcattaaa tttctttttt
     4861 cagacggcct gttgattcgg accttcaggc cgtctgaaac cataaattct gttttattgc
     4921 ttgttatctg aaatctattc gcggtatgaa aaaaattgct gctccgctac catcattccc
     4981 gtatagacgg aaatccaaac ttaaatcttt cagcactctt tacagcagaa catttcaact
     5041 tttgaatttc tgcctgtatg ggaatgacga cagcagagca gcatgttttc caaacggccg
     5101 gctctctctc gcatactgca ctaggccatc gaaaaacgaa aacatcattt attcattgtt
     5161 ttcaaacggc atttcgagat ggggcgggct ggcggcccac cctgctgtta cgcgtattag
     5221 cggtcttcaa tctccacaaa cgccaaatct tcagggcccg tgtagttcgc gctcggacgg
     5281 atgattttgc cgtctttgcg ttgctcgaga acgtgtgcgc tccaaccggt tgtacgagaa
     5341 atcacgaaca gcggtgtgaa catagcggtc ggtacgccca atttttgata ggaaacggcg
     5401 gagaaccagt ccagattcgg gaacattttt ttctcttccc acatcacgct ttccaaacgt
     5461 tcggcaatgt caaagaggcg catatcgccg gtttctttgc tcaaaccgcg tgccacttct
     5521 ttaatgacaa cgttgcgagg gtcggaaatg gtgtacaccg gatgaccgaa accgatcacg
     5581 atttctttgc ggccgatgcg ttcgcggatg tcggcttcgg cttcgtcggc attgcggtag
     5641 cgtttttgaa tatcgtaagc cacttcgttc gcgccgccgt gtttcggacc tttcaacgcg
     5701 ccgattgctc cggtaatgct ggagtacata tcagagcctg taccggcgat cacgcgggcg
     5761 gtaaaggtag aagcgttgaa ctcgtgttcg gcatacagaa tcagtgaaac gtgcatggct
     5821 ttgatgtgtg attcgcttgg gcgtttgccg tgcaacaggt gcaggaaatg accgccgatg
     5881 gtctcttctt cgctttcaac ttcaatgcgt ttgccgttgt gcgaatattg ataccagtac
     5941 aagaggatac tgccgaggct ggcgatcagt ttgtcggcaa tgtcgcgcgc ttcgctttcc
     6001 ggatggcctt cacgttcagg atgaacacag cccagcatgg atacgccggt acgcatcacg
     6061 tccatcggat gggtatgtgc aggcaggctt tccaaaactt taatcacacg gataggcagg
     6121 ccgcgcatgg atttgagctt ggctttataa gcggccagct cgaatttgtt gggtaaatgg
     6181 ccgtgaatca gcaggtgggc aacttcttca aactcacatt tttgtgccaa atccagaatg
     6241 tcgtaaccgc gatagctcaa atcgttgccg gtacggccaa cggtacacaa agcggtatta
     6301 ccggccgcaa cgccagaaag cgcaacggat tttttaggtt tgagggtcgg ggtttgagta
     6361 gtttcagtca tggtatttct cctttgtgtt tttatgggtt tcgggttttc agacgaccga
     6421 tgcggatttg ttgaaaggca gtctgaaagc ggtaaatcat ttttgaaaca atttatccag
     6481 tttttgctcg aaggcatgat agttcagatg ctcgtacagc tcggcacggg tttgcatact
     6541 gtccaccacc gccgcctgag tgccatcgcg cataatcgct tcgtaaacat tcagagcggc
     6601 tttgcttgct gcacggaacg atgacagcgg atacagcacc agcgacacgc cgttttcagc
     6661 cagctcgctt tgggtataaa gcggagtgga accaaactcg gtaatgttcg ccaacacggg
     6721 cactttcacc gcatctgcaa attggcggta catgttcaaa tcggtcatgg cttcagggaa
     6781 aatcatgtcc gcaccggctt cgacacaagc ttgggcgcgt tcgatagcgg catccaaacc
     6841 ttctaccgcc agcgcatcgg tacgcgccat aatcacgaag ttctcatcaa cgcgcgcatc
     6901 tacggcagct ttgatacggt cgaccatttc atctttagat acaatggctt tgttcggacg
     6961 gtggccgcag cgtttttgcg ctacctgatc ttcgatgtga accgctgcaa caccggcgcg
     7021 ttcaaagttg cgaatggtac gggcaatatt gaatgcaccg ccccaaccca catcgatgtc
     7081 caccagcaga ggcgtatcca cgttgtccgt aatgcgtcgt gcgtcgatca gcacatcttc
     7141 cattgtggta atgcccaaat cagggatacc gcaagaacag gctgccacgc cgccgccgga
     7201 cagatagatg gctttgaaac cgctttgggt ggccaatcgt gcaaaataag cattgacgca
     7261 accggcgacg gcaagcggat tcgattcttt cacggcttgg cggaaacgtg ctccggcaga
     7321 gtgttgactc atcatatttc tcctttatag acttttttca gtatggacag gcttccatca
     7381 cattcggacg gcaaaacaca gccatccgac gcgtcgggca tcccaatcca atcattaaaa
     7441 atatatgaga acaattatct tattgatatt taaaacgaat caaagaaaaa cagcagaccg
     7501 ttcggaatta tgcggcaaaa ccgcagacaa gaagaaaaca aggggattat tcagaaaagg
     7561 ggaaaacatc tgaattggtt tcatagtaat gttcctttgt agtattgtgt agttttattt
     7621 ttcgctaatc taaaacacaa gactgaaaat gtcaaacatt tttaaggaga ctcattacat
     7681 aattttaata ttttatttta attccatgat taaactagat taattttgaa ttatttgttt
     7741 caataaaatt gtcaggaaat caagaaaaat ttttgataat caaaatcctg aaaacttata
     7801 aaatatttta tacataaaaa caatgtatta tttttaagca atccgatgta ttttattatg
     7861 tatttgattt ttatttaatt taataaaaat ttaatatacc ggcaaacaaa atgccgtctg
     7921 aaaacctttc agacggcatt tttaatatcg aatcaatcag cccaaatacc agaatttcag
     7981 tgcccacaat acggcaacaa tccataccat aggcggaacg tctttggtgc ggcggcataa
     8041 aagtttaacc acggcatagc tgatgaagcc gaaggcgatg ccgtctgcaa tcgaataggt
     8101 aaacggcatg aagacaatgg tcaggaatgc gggtgcggct tccgtcatat cgtcccaatc
     8161 aatatccctc gcactgcgga gcatctgcgt gccgacataa agcagggcgg gcgcggtggc
     8221 aaatgccgga acacttttcg ccaaaggtga aaacatcagg caggcgagca tcaatacgcc
     8281 gacggtaacc gccgtcaggc cggtccgtcc gcctgccgat acgcccgccg cgctttccac
     8341 ataaggcgtg gtggaagaag tacccaaagc cgcacctgcc acaatggcgg tagagtctgc
     8401 aagcagtgcg cgtttcaggc ggggcagctt accgtccacc agcagcccgg cacggtggga
     8461 tatgccgacc agcgttccgg tactgtcaaa tagatcgacc aagaagaaga cgaaaatcac
     8521 actgaccatg ctgacggtaa acaggccttc aaaatccatc tgcataaaag tcggcgcaat
     8581 gctcggtact tcgccgatga tgccgtgaaa ttcattcaaa cccatcaggc tggcaatgac
     8641 ggtaatggtc aagatggtga tgatgattgc gccttgaacg cggaaatgtc ccaatacgac
     8701 caccatagca aaaccgaaca gtgccaacaa cgcggacggc tgatgaatat cgcccaaacc
     8761 gaccaaggtt gccggattgg caacgataat gcctgcgcct ttcagggaaa tcagtgccaa
     8821 aaacaaaccg ataccggcag caatcgacat tttcaaaccc ataggcagtg cgttgaccag
     8881 catttccctg actttaaaaa agctgaacag gatgaaaatc agaccggaga tgaacaccgc
     8941 acccaacgca acctgccaag gcacgcccat acccttaacg acggcaaagg tgaaataggc
     9001 attcagcccc atccccggtg cgagtgcaat cggatagttg ccgacaaaac ccataacaaa
     9061 acagccgatg gcagacgcga tacaggtagc gacgaatacc gcccccatat ccatgccggt
     9121 ctcgcccaaa atcagagggt tgacgataac gatgtagcac atcgtcaaaa aagttgtcaa
     9181 acccgccatc aactcggtac gcaccgtcgt accgtttgcc ttcagcttaa aaatcccgtc
     9241 caacagtgtt tgttttgaag tgtccatatc tgaaagtctg tatcaagtgg aaaaatctga
     9301 atccgccgac cgcaaaccgt acgaaattgc aacatcttga aaaacatacc tcccaaaccg
     9361 gacaaaccga ttcgggaggc agcaggttta cgggcaaaca gcacattatt tgtctgaatt
     9421 gtgttgacga cgcaaaatcg cagggatttc aaagtcgtca agtacggact gattgtcgaa
     9481 atccgcagcg gtaaggttca tcgtgcggat accgcgattg gtgcggatca taccttcgac
     9541 attatggctt tgctcctgtt tggacggggc aaccgcttct acctcccttg ccggaacaaa
     9601 atcgaccgcg cctttttctt tcagaccggt agcgataatg gtaatccgga tggcatcttc
     9661 gctcatggtc tcgtcttcag ccgcaccgaa tttgcattcc aaatcgggat gcgcgctttg
     9721 gttgacgatt ttcatgactt cggacaactc ggacattttc aagcaacccg gagcagtcgt
     9781 aatattgacc agcacaccgc gcgctccgtc caaggttaca tcgtccagca gcggactgga
     9841 aatggcctgg tcggtcgcca tacgcgcacg gtcgatacct tgggcataac ccgaacccat
     9901 catagcgata ccgcggttgc tcatcacggt tttcacgtcg gcaaagtcga ggttgatgat
     9961 ttcgctcggg caagttacca cttcggaaat gcctgcgacc gcatcgcgca atacattgtc
    10021 ggcggcacgg aaggcttcgc gcatcgttac gtcttcaccc aatgcagtca tcagtttgtc
    10081 gttcgggatg atgatcagcg aatcgacgtg ttctttcaac tgttccaacc ctgcctgtgc
    10141 gacatggacg cgcttacctt catatgcgaa cggtcgggta accacggcaa cggtcagaat
    10201 gcccaaagac ttggcaatct cagcaacaac cggcgcggaa ccggtaccgg taccgccgcc
    10261 cataccggtc gtgataaaca gcatattcgc accgcgaatg gcttcttcaa tggcttcccg
    10321 gtcttcctgg gctgccgcac ggccgatatc gggattcgcg cccgcgccca aaccgcgtgt
    10381 cagattcgta cccaactgga ttctcttcgc cgcatggttt tttgccagag actgcgcatc
    10441 cgtattggca ctgataaact ccacaccgcg cacattgttg gcaaccatgt tattgattgc
    10501 attgcaaccg ccgccgccca agccgattac tttaatcacc gcagggctga ctgccgattc
    10561 tgccacgtcg taaacaaatt ccattcaaaa actcctgctc gccccattca gaggacggtt
    10621 taaataaatt attattcatt atataagaag tatcttgctg ctggcaaaat acttctcacc
    10681 tgtcaaacgg caatccacct gttcagaagc tgttttcaat ccaccgttgc aatcttgcca
    10741 acaaaccgcc gcccccttcc ctctcttgca ctgcaccgtt ttccggctgc ggcaagtttc
    10801 cttccagctt gcatgctgca tgaagcagcc cgatagcggt agaaaaacgc ggtgtgcgga
    10861 cgcggtcgga caaaccgccc atttcttggg gtgcaccggt gcgtacaggc aaatcgaaga
    10921 ttttttcggc aaattccaca atcccggtca tcatggacac accgccggtc agaacgatac
    10981 ccgcattcag cacttctttg gggaaacccg atttttgcag ctcgcccagc actacgccaa
    11041 aaatctcctg aatacgtgca ctgatgattg ctgccaaaac cttactggaa acctgacgcg
    11101 atgtccggtc acccacgccc ggaacttcaa tcatctcacc caagccttcc gtatcgcatg
    11161 atgccacgcc ataatggatt ttaatgtact cggcggcatc gagaggtgtt ctcaacgatt
    11221 tggacaaatc tttggtaatc agattaccac cggccggaat gacggacgta tggcggatgg
    11281 cgccgttcat ataaacggca atatcggtcg ttccgccacc aatgtcgatg acgcatacac
    11341 cgaggtcttt ttcatcttca gtcagcaccg cctgcccgct tgccaacggc tgaagcataa
    11401 tctgatcgct tttcaaaccg caccgctcga tacatttttg gacattctgc actgccgtac
    11461 ttgcaccggt aatgatgtgc acccgcgtat ccagacgcac accgctcata ccgatgggct
    11521 ccctcacgcc aagttgggtg tcgataatgt agtcttgaac cacggcatcg agaatttttt
    11581 gatcgggcgg gatattgatt gcctttgccg tttcaatggc gcggtcgatg tctgcctgcg
    11641 tgacttcccc gtctttaatt ttaaccacac cttgcgaatt gagactgcgg atgtggttgc
    11701 ctgcgatacc tgtggtaacg tgggtaattt tggtatccgc catcagctcg gcatcattga
    11761 ccgcctgcct gatggcttgg acggtggcat cgatattggt taccatgccc gcgcgcaagc
    11821 cccgtgaagg agcctgcccc aaaccgacga tgttgatttt gtcgtcatct tgaacttccc
    11881 cgatcagtgc gaggacttta gacgtaccga tatccagtac gctgatgtat ctttgctgct
    11941 gttccattgt tcgtctgctc ttaaaactga ttgaaatttg cgtcgcaccg tttcagacgg
    12001 cacggccgta atctgtccga tacctgttcc cactattctt cggattcttt ttcgggtaaa
    12061 ccgtcggaag catagcggac tgaaaatccg tccttatacc tcatatccac ataggataac
    12121 cgatttttat ttttacgcaa cagatgctgc cacgcttcgg taaaaagccg gaggcgtttc
    12181 atctcgtttt cccgtccgag cctgacggtg atgccgttgt ccaaaacgac aatccacgcc
    12241 gaacgtgccg tataggtcat ctctttgatg cccaaaccct gttttgccaa aacagtcgaa
    12301 aattcgtcat aacggcggag catttcggca gacgttcctt ccgcgcctct gaataccggc
    12361 attccgggtc tgtccaagcg ggcttcaaaa acattgcctt cgccgtccac caaggcatgg
    12421 tcgccccaac gcgcgaccgg cttgcgctcg gtcaggacga cctcaaccgt gtcgggaaaa
    12481 cggcggcgca ccatgaccga cgcaatccac ggataccggc ggtaggcctc ctgtgcgcca
    12541 ttgatgtccg tcctcaaaat attcccatgg atgtattctt tcgccaaact gcccaatgcc
    12601 ttcttatcgg aataaaccag gttgcccttc agcgacacct gcttgacggg cagatgattc
    12661 gaattgtaaa accaaaccag cccggacgca gcaagcagca tcgccatcat gacaagcagc
    12721 cagcgcgtca gccgttccat cgcttcggca ttatcccaca tgtgcggtct tcaaaatttc
    12781 aatacataaa tcggcaaaac ccacgcccgt aacggcagcg gatttcggta ctaaactatg
    12841 gctcgtcata ccgggcaggg tgttgatttc caacagatag agtttgccgt cggtatcttt
    12901 gaggaaatcg acgcgcacgc agccttccgc accgattgcc tgcgcgccgc gaaccgccag
    12961 ttcgcgcatc aggctttctt cggcttcggt caaatcttcc gaaggacatt gataaatggt
    13021 gtcgtcgcgg ttgtacttgg cttcgtagtc gtaaaactcg gttgcgggaa tgatgtgtat
    13081 gccgggcagc cctttgccgt tcaggacggg gcaggaatat tcgccgccgc cgataaaacg
    13141 ttcggcaatg atttcgccct gaaggtgttt caattcttcg taaacgcttt tcagacggcc
    13201 tttttctttg acttttacca caccgacgct gctgccttcg gccgccggtt tcacaaacat
    13261 cggcaggccc aatttttctt cgacggcatc gaaatcagtg tcgtcgtgca ggacggcgaa
    13321 ctcgggaacg ggcaatccca atgcctgcca aatcagtttg cagcggtatt tgtccatgcc
    13381 gatggcggat gcggcgacac cgctgccggt atagggaatg cccaacagtt ccaatgcacc
    13441 ctgaaccgcc ccgtcttcgc cgtaagtacc gtgaaggatg ttgaatgccg tctgaaaacc
    13501 ttgtgccttc aattcagaca atggggtttc tttaggatcg aaggcgtatg cgtctatgcc
    13561 tttgcttttt aaagcattca aaatggcggt gccgctgtcc agcgagattt ctcgttcgct
    13621 ggaaaaaccg cccatcaata cggccacttt gccaaaattc tgcattgttt tttgttcttt
    13681 cctgattgct ttatgcttgt tgccagaggt cgtctgaaac ctaatttgcg gtttcagacg
    13741 acctttatat gatgttccgt ctgtcaggcg ggtgtgcctc aaatctgttt cgacaattcc
    13801 agtagcgcgg cggggacgcg gttgatgctt cccgcgccca tattcaacac gatgtcgccg
    13861 tcctgcaaaa cgttcaacag catttcgggc agatcggcaa cgttttcgca gtaaatcggc
    13921 tcgagtttgc ccaacacgcg gatggcgcgg gcaagagcgc gggaatcggc ggcggcaatc
    13981 ggctcttcac cggcggcata aacttcggtc agcaccagcg cgtcaacggt attgaggact
    14041 ttggtaaagt cttcaaacaa atcgcgcgtg cgggtatagc ggtgcggctg gaaggcgagt
    14101 accaaacgtt tttccggata cgcgccgcgt gcggcggaaa gggtcgccgc catttcgacg
    14161 gggtggtgtc cgtagtcgtc caccaagagc gcggtcccgc cgtttggcaa cttgatgtcg
    14221 ccgtattttt ggaagcggcg gccgacgcct tcaaagccga gcaagccttt ttggatcgct
    14281 tcaaccgatg cgccgacttc cagcgccacg ccgatggctg ccaatgcgtt cagcacgttg
    14341 tgtctgccgg gcatattcag cacgacttca aacgacccct gctcatgtcc tttcatttga
    14401 acatggacgg tgaatttcat ttgcgcgccg acgttttcga tgtcggtggc gtagatgtcg
    14461 gcggtatcgt ccaaaccgta agtagcataa ggtttgctca ctttgggcaa aatcgcgcgg
    14521 acgtgttcgc tgtcaataca caaaaaggct ttgccgtaga agggcatacg gtggatgaaa
    14581 tcgataaacg cctgatgcag tttttcgacg ctgtgcccgt aggtatccat atggtcttcg
    14641 tcgatattgg taacgacgga cataatcggt gtcaggtgca gaaaggatgc atccgactcg
    14701 tcggcttcgg caacgatgta ttcgcctttg cccaagcggg cgttggtgcc tgcggcgttg
    14761 agtttgccgc cgataacgaa agtcgggtca agtcctgccg cgccgaggat ggaggcggtc
    14821 aggctggtgg tcgtggtttt gccgtgcgtg ccggcaatgg cgatgccgtc acggaagcgc
    14881 atcaactccg ccaacatcag ggcgcgcgga ataacgggaa tttgctgctc caacgcagcg
    14941 acaacttcgg gattttcttt tttgacggcg gtagaggtaa cgacgacatc cgcaccgtta
    15001 acgtgttctg cggtatggcc gggataaact tgaatgccca ggctgcccaa atgctcggta
    15061 gcggcatttc gcgcctgatc cgaaccggaa actttaaaac ccaaattgtg caagacttcg
    15121 gcgatgccgc tcatgccgac gccgccgata ccgacaaaat ggatgttggt aactcgattt
    15181 ttcatcataa tgttgcgttc cggtggattt tcgatgcgta aaggcgttat tttaaagggc
    15241 tatccgcttc tacgccatag ttttcttgta aattacagat gtttcggcat acaggatttc
    15301 atatcgatgg caatatagtt aaaaataagg ctgcacccag cggcaaaccc tgctttcctg
    15361 cccattttca aactcgattt catataccga aacaccagaa aaaacaaaat ataaggccgt
    15421 ctgaaaccgg tttcgtggtt ttcagacggc atctgtttcg tttatcgcat catgtcatca
    15481 tgccgtacac tcgatagcgg cttccgccac gtcgtccgca ctgtgcggca gtgccaacgt
    15541 acgggcgttt tctgcccatt tgagacattt ttcgcggttc aggctgccga ggatttcggc
    15601 gagtttttcc gccgtcaact gggtttgcgg caacagcaat cccgcctccg cctgcaccat
    15661 aaaacgtgcg ttggcggttt gatggtcatc aacggcgtga ggatacggca ctaacaacgc
    15721 acccaatccc gccgccgtca actcggcaat cgtcagcgcg ccggcacggc aaatcaccaa
    15781 atcggcatcg cggtaggcgg acaccatgtc ggtaataaat tccacgcatt ccgcttgcac
    15841 gcccagcgcg tcgtaatccg cctgcaagct gcccagcttg ccccgtcccg attggtggta
    15901 catctgcgga cgcgcattgt cgggcagcaa agccaatgcc tgcggtacgg ttttgttcaa
    15961 aacgtccgcg cccaaactgc cgccgaccac caaaattttc agacggcctt cacgcccttg
    16021 gaagcgttcg gcaggcacgg gcaggttgct aatatcggcg cggacgggat tgccgaccaa
    16081 accgccttcg tggctgaacg ctttcggaaa agcgtacaac acccgcttcg cccagcgcga
    16141 caggtggcgg ttggacaaac ctgccacggc gttttgctcg tgaatcacaa tcggcacgcc
    16201 taataacttc gccgccaaac cgccggggaa ggtaacgaag ccgccgaagc cgatgacgca
    16261 ctcgacacgg tgtttgcgga taatccgctg cgcttcgcgg acggtttgat acaaagtaaa
    16321 cggcagcatc agcttgcgtt tgatgccgtt gccgcgcacg cctttaatcg ccagcgtttc
    16381 caagcgtatg ccgtattgcg gcacgatacg ctcttccatc gaatccttgc tgcccagcca
    16441 aatcacatga tggccgcgcg cgcgcaatga atccgccacc gccagcgcgg ggaaaatatg
    16501 tccgcccgtt ccgcccgcca tcagcataaa ggttttaccg cccatgattt actccacccg
    16561 ataaccgcgc attttccggc ggttttcata atctatacgc aacagcagca tcatgctgat
    16621 cagcatgaaa aagactgacg aaccgccata ggacatcaac ggcagcgtca gacctttggt
    16681 cggcaaagca ccgatgttca caccgatatt gaagaaactt tggataccga tccaaatgcc
    16741 gatacccgag gcgatatagg cgttgaaagt caaacccaaa tcgcgcgact gcttgccgat
    16801 ggaaaacgcc cgcaccacca gccagccgta acagaatatc agcacacaca taccgaagaa
    16861 accgaattct tcggcgatga tggcaaaaat aaaatcggta tgcgcttccg gcagaaagcc
    16921 gcgtttgctc aaactcgcac ccaaacccat accgaaccac tctccgcgcc cgattgccat
    16981 cagagagtgg gtaagctggt agccggcacc ctgagggtct ttccacgggt ccaaaaatgc
    17041 cactacccgc tgcacacggt agggagcggc ggtaatcatc agcaccatcc cgcccaagac
    17101 actgcctacc aggacgaaaa aatatttcca cggcaatcct gccaaaaaca gcattccaac
    17161 ggcaatgacg gtaatgacga caaacgaacc gaaatccggc tgtaccatta tcagcaccaa
    17221 accgaacgcc accagcataa tcggcaggat gatcgcccgg aaacggccgt acatttctaa
    17281 tgtttcacga cgtgcctgcg gattggtggc ggacatgatc agattggccg tcccccgcca
    17341 aatcgactgc caacccaaac tttccatgct gcgcaacact tcttcacggc gcgtgaacag
    17401 gcttgccaaa taaaggatga ccgccagctt gaacagctcg gtcggctgga aattcaacgg
    17461 acccaaaggt atccaacggg tcgcgccatt gatttcgcgc ccggcaatca atacgactac
    17521 cagcaacagg ccggataggg caaaaatcca cggcacaagc cgccgccatg tcctcatcct
    17581 gcaaagaaac cataacaaac cgctcgctat caagccggca acgacgaacc ccgcctgtct
    17641 ggtcaaatag aaaaactgat cgccgccttc ttttgatgcc aaatacacag aagccgaata
    17701 aatcatcagc aggctgaacg ccgtcatcag caccaccatc cacaaaagcg gcgcgtcgaa
    17761 tttcctgccg tcgcgcacaa tcggcctgtc gagcagcaga gtgtggacac cgtcgcccac
    17821 ttttaccaat acttccgaaa tcttcaaaaa aaccacctgc cagtctgttt gcacctgccg
    17881 caaagggcaa aaatttcaga cggcagacaa tgccgtccga acatacgata catcccaaat
    17941 cggtattcta aatctttact tgccgcccaa caatgacggc gtttgcattt cagacggcat
    18001 cacaaagcct taaacgcttc gataaacact tccgaacggt gcgcgtagcc tttaaacata
    18061 tcaaaactcg cacaagccgg gctgagcagc acgatatcgc acgcttcggc ttgggcatat
    18121 gccgtctgaa cggcttcttc caaagtggcg cagtcggtca tattcagact gcagccgtcc
    18181 aaatcgcggc ggatttgcgg cgcatcaacg ccgatcagga acacgccttt tgccttgccg
    18241 gcaagcgcgt cgcgcagagg cgtgaagtcc tgccctttgc ccatgccgcc taaaatcacg
    18301 aagagcggat tttgcaaacc ggcaatcgcg gcggcggtcg caccgacgtt cgtgcctttg
    18361 ctgtcgtcga tgaacaccac gccatttttc tcgccgattt tttccacgcg gtgcggcagg
    18421 ccttgaaagg ttttgacgtg ttcaagcaat gcttcgcgcg gcaaaccgac ggcttcgcac
    18481 aaagccacgg cagccatgac gttggcggcg ttgtgcaagc cttgcaacgg aatgtctttc
    18541 gtggcaatca aatcttcatt gccttgtttc aggcggcctg tctcacgttc caaccaaaaa
    18601 tcagcttcgt gttccaacga aaaccatttt acctcgcgcc ctgcccgttt catcgcgcga
    18661 cagaacgcat catccacatt caaaacctgc acgccatcgc cacggaaaat cttggctttg
    18721 gtatgcgcgt agtcgagcaa gtcgtcgtag cggtcgagat ggtcttcgga aatgttcagc
    18781 accgtcgccg cagtcgggcg caggctttcg gtgttttcca gttggaagct ggaaagctcc
    18841 aacacccaca cgtccgcctt tttgccttcg cgctgccatt ccgcctccaa aaccggcgtg
    18901 ccgatattgc ccgcgataac ggtatccagc ccgcacttga tacagagata gccgaccagg
    18961 ctcgttaccg tggttttgcc gttgctgccg gtaatcgcaa ttaccttgtc gccgcggcgg
    19021 ttcacaatgt ccgccagcaa ttcgatgtcg cccaacacgc gtccgccgtt ttgcttgaac
    19081 gcctcgatat ccggctgccg ctcgctgatg ccgggactga gcgccagaat atcgaaaccg
    19141 ttgtccagcg catctttcag acggcccgtg taaaacacca gcccgtcaaa catcttaccg
    19201 atttgcgaca cgcgttccgg cttcagctcc gcatcatacg cagcaacctc cgcgccgttt
    19261 ttgcgcaggt aggcaatcat ggaaataccc gtgccgccaa gtccggcgac gaggattttt
    19321 ttgttttgga aagtcatttt ggtttgtcct aaaacaaatc atattgggca gggggatgtc
    19381 tactcctgct caagccgctt tcagacggca ttgcgagctg ttcaataacc cgctttcagg
    19441 cgttggtcat tgtcgcagcc gtcttggttt ccgttttgac acgccttgcc aagccattct
    19501 tgtgcaaggg cgcggtcttg gcgcacgcct tcccctttga gatacatcag acccaaatta
    19561 tgttgggcta cggcatcccc ttgttctgca gccctacgat accactgcgc tgcccgttta
    19621 taatcccgac gaactccttc gcctttgtaa taccttatac ccaaattatg ttgggcttgg
    19681 acaatccctt gttctgcagc cttgcgaaac cactgcgctg cctgtttaga atcccgacgg
    19741 actccttcgc ctgtgagata catcagaccc aaattatgtt gggcttctgc cttccctcgc
    19801 tccgcagcct tgcgaaacca ctgcactgcc tgtgcatagt cttgacggac tcctgcgcct
    19861 ttgtgataca tcacacccaa attgtattgg gcttcggcaa tcccttgttc tgcagccttg
    19921 cgaaaccact gcattgcctc tgcatagtct tgacggactc cttcgcctgt gagatacatc
    19981 agacccaaat tatgttgggc tgcggcatcc ccttgttctg cagccttgcg aaacctctgc
    20041 attgcctgtg catcatcttg acgaactcct tcgcctttgt catacatcac gcccaacaaa
    20101 acttgggctt gggcatcccc ctgctccgcc gcctgccgat accatttgac cgcttcggca
    20161 tcgtcttggc gcacgccttg tccgatggca tacatactgc ccaacaaagc ttgggctggg
    20221 gcaaacccct gcgctgccgc ctgccgatac catctgaccg cttcggtatc atcttggcgc
    20281 acgccgcgtc ctttgtaata cattgcgccc aaattgtatt gggctgctgc atctccctgc
    20341 tctgccgcct gcaagttttc ccgaaaatcc gatacgtcat ccgcccacac cgcttggttc
    20401 aagcccaagg caatcagggc ggctgcaagc catttgactg tctgtttcat gttttctctc
    20461 ctatttcggt atggggcggt ttttcagccg ccgttaacga taagacggga cagattaccg
    20521 ccgcaggttt gcacgttcaa atgccgtctg aaaggtgttc agacggcata ggttcagcga
    20581 attttgaggg tactcaagcc gatcagcacc aagacgatgg taataatcca gaaacggaca
    20641 acgacttggg tttctttcca gcctttttgt tcgtagtggt ggtggatggg cgccatcagg
    20701 aagatgcgtt ttttggtttt cttataccag ccgacctgaa gcataacgga tacagcttcg
    20761 acgacaaata atccgcccat aatcactaaa acaaattctt gacggacgat gacggcaacg
    20821 gtaccaagcg cggcacccaa tgccaatgcg ccgacgtcgc ccataaagac ttgcgcggga
    20881 taagcgttaa accacaaaaa tccgaggcac gcgccgcaca tggcggtgca gaatatagcg
    20941 acttcgttcg caccggcgac atagggaagc tggaggtatt gggaaaattg gtagtgtccg
    21001 ctgacgtagg cgaaaatggc gagcccggcg gcaacgagga cgacggggaa ggcggcaagg
    21061 ccgtccaagc cgtcggtaag gttgacggcg ttggatgtgc cgacgatggt caggtaagac
    21121 aacaccaaaa agccgaccac gcccagcggc aggacaattt gtttaaagaa tgggacaatc
    21181 aaaatattgt tggcggaatt ggcggcaagg taaaacaatg ccaaaccggc gataacggca
    21241 acactcgact gccacaccat tttgaatttg gcggacacgc cgttggggtc tttatagacg
    21301 actttgcgcc agtcgtcgta aaaaccgagc gcgccggtgg caagcaagac gcccaagaga
    21361 atccagatat acgggttcgc ccagttgccc cacaacaggg tggacacggt aatggcggtc
    21421 agaatcagcg aaccgcccat cgtcggcgtg ccgtttttga cgaggtgggt ttgcgggccg
    21481 tcggtacgca ctgcctgccc gcatttgagc gcggtcagcc tgcgtatcgt ccacgggccg
    21541 aacatcaggg aaaacgccaa ggcggtcaac gccgccatga cggcgcggaa tgtggtgtat
    21601 tgaaaaatat tcagaccggt taaccagttg ctgaaatgtg cgagccataa aaacatgggg
    21661 cttccttttt ttgttttgtc
//
LOCUS       DAWGJV010000015        21610 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWGJV010000015 DAWGJV010000000
VERSION     DAWGJV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21610
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.015"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(48..1229)
                     /gene="odhB"
                     /locus_tag="WHQ94_000412"
     CDS             complement(48..1229)
                     /gene="odhB"
                     /locus_tag="WHQ94_000412"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ2127750.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1329..4157)
                     /locus_tag="WHQ94_000413"
     CDS             complement(1329..4157)
                     /locus_tag="WHQ94_000413"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ2127751.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNASKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNGSVKRVVLCAGQVYYDLEAGRTERKLEDDVAIVRVE
                     QLYPFPYEEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4362..5645)
                     /gene="gltA"
                     /locus_tag="WHQ94_000414"
     CDS             complement(4362..5645)
                     /gene="gltA"
                     /locus_tag="WHQ94_000414"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ2127752.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(5757..6005)
                     /locus_tag="WHQ94_000415"
     CDS             complement(5757..6005)
                     /locus_tag="WHQ94_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ2127753.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6009..6716)
                     /locus_tag="WHQ94_000416"
     CDS             complement(6009..6716)
                     /locus_tag="WHQ94_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ2127754.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDDLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6836..8599)
                     /gene="sdhA"
                     /locus_tag="WHQ94_000417"
     CDS             complement(6836..8599)
                     /gene="sdhA"
                     /locus_tag="WHQ94_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ2127755.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGEDYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8602..8943)
                     /gene="sdhD"
                     /locus_tag="WHQ94_000418"
     CDS             complement(8602..8943)
                     /gene="sdhD"
                     /locus_tag="WHQ94_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ2127756.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(8937..9314)
                     /gene="sdhC"
                     /locus_tag="WHQ94_000419"
     CDS             complement(8937..9314)
                     /gene="sdhC"
                     /locus_tag="WHQ94_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ2127757.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9593..10996)
                     /locus_tag="WHQ94_000420"
     CDS             complement(9593..10996)
                     /locus_tag="WHQ94_000420"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2127758.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVSQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11245..11982)
                     /locus_tag="WHQ94_000421"
     CDS             complement(11245..11982)
                     /locus_tag="WHQ94_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ2127759.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12261..14537)
                     /gene="metE"
                     /locus_tag="WHQ94_000422"
     CDS             complement(12261..14537)
                     /gene="metE"
                     /locus_tag="WHQ94_000422"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ2127760.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     QYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14675..15553)
                     /gene="metF"
                     /locus_tag="WHQ94_000423"
     CDS             complement(14675..15553)
                     /gene="metF"
                     /locus_tag="WHQ94_000423"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ2127761.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15753..16028
                     /locus_tag="WHQ94_000424"
     CDS             15753..16028
                     /locus_tag="WHQ94_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ2127762.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16028..16153
                     /gene="ykgO"
                     /locus_tag="WHQ94_000425"
     CDS             16028..16153
                     /gene="ykgO"
                     /locus_tag="WHQ94_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ2127763.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16259..16459)
                     /locus_tag="WHQ94_000426"
     CDS             complement(16259..16459)
                     /locus_tag="WHQ94_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127764.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16602..17741
                     /locus_tag="WHQ94_000427"
     CDS             16602..17741
                     /locus_tag="WHQ94_000427"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ2127765.1"
                     /translation="MSQNASVGIVMPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLSIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            17738..18319
                     /gene="metW"
                     /locus_tag="WHQ94_000428"
     CDS             17738..18319
                     /gene="metW"
                     /locus_tag="WHQ94_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ2127766.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18436..19281
                     /locus_tag="WHQ94_000429"
     CDS             18436..19281
                     /locus_tag="WHQ94_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ2127767.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPNKEDKTKETGASAASSPASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLTQGSEETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19330..19890)
                     /gene="efp"
                     /locus_tag="WHQ94_000430"
     CDS             complement(19330..19890)
                     /gene="efp"
                     /locus_tag="WHQ94_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ2127768.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(19933..21081)
                     /gene="earP"
                     /locus_tag="WHQ94_000431"
     CDS             complement(19933..21081)
                     /gene="earP"
                     /locus_tag="WHQ94_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ2127769.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTALVPDIVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSLQESVQKYFWFMGFSEKSGGLIRERDYRNA
                     VRFDTEALRERLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21100..21378)
                     /locus_tag="WHQ94_000432"
     CDS             complement(21100..21378)
                     /locus_tag="WHQ94_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127770.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5342 a         5494 c         5458 g         5316 t
ORIGIN      
        1 aattcagacg gccttttcat taacaaataa aaggccgtct gaaacgatta cagatccaac
       61 aacaggcggg ctgggtcttc caacgcgtct ttaatggcta ccaaggtcaa tacagcttcg
      121 cggccgtcaa tgatacggtg gtcgtaagac agagccagat acatcattgg acggacaaca
      181 acttgaccgt tttcaaccac agcgcgctct ttagtggcgt gcatacccaa aattgcagat
      241 tgaggcgggt tgatgatcgg ggtagacatc atagaaccga aagtaccgcc gttggtaata
      301 ctgaatgtac cgccggtcag atcttcgata gcgattttgc cgtctttggc ttttttcgcg
      361 taatcaacaa ttgcttgttc gatgtctgca atgctcattt ggtcggcatc gcgcaggatt
      421 ggcacaacca aaccgcgtgg gctgccaatc gcgataccga tgtcgaagta gccgtggtaa
      481 acgatgtctt tgccgtcaac agaagcattc acaaccgggt attttttcag ggcggcaaca
      541 gcggctttaa cgaagaagga cataaagccc aatttcacgc cgtgttcttt ctcaaatttt
      601 tctttgtact tcgcacgcaa gtccatgata ggtttcatgt tgacttcgtt gaatgtagtc
      661 agaatggcgt tttcttgttg agaagccaag aggcgttctg caacacgggc acgcaggcgg
      721 ctcattggta cgcgttcttc aggacgtgcg ccggcaggaa gtgcaacagc aggagcagcg
      781 gctgcggcag gtttggcagc ggcattttgt acgtcttctt tcaatacgcg accgtcgcgg
      841 ccggaacctt gcaatgcgtt cacgtcaaca ccggtctccg cagccagttt ggcggcggca
      901 ggcatagcgg cgttgttttg tgcggcagca ggagcggcag ccggggcggc ttcggcagga
      961 gcggctacgg gtgcttcggc agcggcagta gcagctgtat cgatgcgtgc caaaacttgg
     1021 tcggcaacaa cggtttcacc gtcttgcgct acgatttcaa ccaatacgcc ggcttgcggg
     1081 gaagggactt ccaaaaccac tttgtccgtt tcgatatcga tcaggatttc gtcacgggca
     1141 actgcttcgc caactttttt cttccattcc aagagcgtgc cttcagatac gctttcagac
     1201 agcataggta cttttacatc aataatcatt ttgtgtctcc aatggccctt tcagacggcc
     1261 tgttgtgtgt ttttcattct gcatatgggc attccgcctg agcagcctca gacggaatgc
     1321 cacttggctt acaacgccaa agcgtcttca accaattgtt tcaattgggc aatatgtttg
     1381 cttgagtagc ccactgcagg cgatgcgctg cttggacgac cggcataaga cagtttttgc
     1441 tcttcgctga taacgtcttc gatgcggtgg cggatttggt agaacgcacc ttggtttttc
     1501 ggctcttctt gtgcccaaac cacagatttt gcgttcggat atttcgccag ttcggcttta
     1561 acctcctcgt atgggaacgg atacagctgc tcaacgcgga caatcgcaac atcatcttcc
     1621 agtttacgct cggtacggcc tgcttccaag tcatagtaaa cctgaccggc acacaatacc
     1681 acacgtttca cgctgccgtt gcttgcgcgt tcggcggtat caccgataac cggacggaaa
     1741 gtcgaacctt cggtgaagtt ttccagcggg ctcattgcac ctttgaagcg caacaggcgt
     1801 ttggacatga aaatcaccag cggtttgcgg tatgaaccca aaacttggcg ttgcaagagg
     1861 tggaacattt gcgacgcttc agacggcatg atgacttgca tattgttctc ggaacacagt
     1921 tgcaaccaac gttctacgcg tgcagaggag tgctcggggc cttgaccgtc gtagccgtgc
     1981 ggcaggatgg tggtcagacc gcacaaacga ccccatttgg tttcgcctga agacaggaat
     2041 tggtcaatag tcacttgcgc gccgttggcg aagtcaccga attgagcctc ccaaatggtc
     2101 agcttgtcag gcgcggagca ggcaaagccg tactcgaacg ccatcacggc ttcttcgttc
     2161 aaaatggagt cgataaccag gaactcgccc ataccttcgc ccatatggcg cagagggaca
     2221 taagtaccgt cgtcccattt ttcgcgtttt tgatcgtgca ataccgcatg acggtgtgag
     2281 aatgtaccgc gcccagagtc ctcgccggaa atacgcacgc catggccttt ggttacgagg
     2341 ctggcgtatg cgagggtttc cgccataccc cagtcgatgg cttgtttgcc ggatgccatg
     2401 gctttacgcg cttcaatcac acgttttgca gtcggatgca gggcaaagcc ttccggtacg
     2461 gcggtaaact tctcagtgag acgctcaata tctgcggcag gcaaaccggt ttcgatgtgt
     2521 tcgcgccaat ctttgccttg gtatttgctc cagtcgattt gcgtgcgttg aaagttgctc
     2581 aacgttgttt gctcgacgtg ttcgcctttg tccaaagcat cacggtaggc ttggatgtaa
     2641 ccgtctgcct cggcttgggt taccacgcct tcggcaatca gttgctcggt gtacaaagca
     2701 cgcgcaccgg ggtgttgcga tacttttttg tacatcatcg gttgggtcaa ggtcggatca
     2761 tcgccctcgt tgtgacccca tttacggtag cagacaacgt caatcacgat gtctttatgg
     2821 aattttttgc ggtaatccaa agcggcttgg atggcaaagc aaacacgctc aggatcatcg
     2881 ccgttcacat ggataaccgg agcggaaacc atttttgcga tatcggtaca gtgtacggtt
     2941 gaacgggtat cgcggatatc ggaagtggta aagccgattt ggttgttgat gacgatatga
     3001 accgtaccgc cggtggtata accgcgcgtt ttagacaggt tgaatgtcgc ttggttgact
     3061 cccagaccga taaatgcgga gtcgccgtga atcaataccg gcaagacttt gtcgcggccg
     3121 ttttcgccca aacgtttttg tttggcgcgc gcagaacctt ccaccaccgg gttgacgatt
     3181 tctaagtgag acgggttgaa cgccaaagaa acatgcatcg ggccgtgcgg ggtagcaata
     3241 tcggagctga agcccatatg gtatttcacg tcgccgctgg gcagtttgat ttcggcacga
     3301 ccttcaaatt cggcaaacaa atcgccgggt tttttgccca aaatgttcac caaaacattc
     3361 agacggccac ggtgcgccat accgatgatg acttcttcca cgccgtcttt actggcgttt
     3421 tgaatcaggt agttcaaacc ggcaatcgcg ctttcgccgc cttcgacacc gaaacgtttc
     3481 tgaccgacat atttggtatg cagataacgt tccaaagttt cggcagcagt catctctttc
     3541 aagatacggc gtttttgatc ggcattgtaa tgcggtgtgg acaatacgct ttcaaaataa
     3601 ttgcgtaccc agcggcgctc ttcggtattg ggaatataga tatattccaa tgcgatgtgg
     3661 ccgcagtagg tttgtttgag gttgctgatg atttgggaca aaggcagttt gccgcgattg
     3721 gcaaaatcgc cctcgcccat attgaattga agcgtcatat cggcatctga cagaccgtgg
     3781 aatttcggat cgagggcttc aatatcgcgc ggggggatac gtttgagcgg atcaagttgg
     3841 gctgcaccca cgccttggat acgataggca gaaatcagcc gtaaaacgct gacttgcttt
     3901 ttcagcattg cctcatccgc accgcccgca acggcagatg caattttctt tttcgccaaa
     3961 gtaacaaatg attcgcgaat cggtgtgtgt gcgacatcga cagcaaccgt ccccggctgt
     4021 ttgctcaaat cggtgaaata ctgcttccat ttttcatcaa ccgcatcggg gttttccaaa
     4081 aaagcctcgt acaattcctc aatgtagggt gcgttcgaac cgaacaggta agagaaattg
     4141 agtttttcgt ccatcatggc gtgcgctctt ttctttaaaa tataaacaaa gggtagaagt
     4201 ccgcgtcctg acggctgaac tgatgccgtc tgaaaagaca ttttattcta ccctatttta
     4261 aaccggattg cctgtattga tttcatccga ctacaatcaa atgtatacag aaagcaatcc
     4321 gattctctaa tattgcctgt ttgacaatat attaatgtat gttacctctc tcctgccggc
     4381 acataatcgc ggcgttccga accagtataa agctggcgcg ggcggccgat tttcagcgaa
     4441 ggatcgctga tcatctcgtg ccagtgcgaa atccaaccta cgctgcgcga cagggcgaag
     4501 atgacggtaa acatttcggt cgggatgccc agcgcggaca ggacgatgcc ggaatagaaa
     4561 tcgacgtttg gatacagctt gcgttcgata aagaacgggt ctttcagcgc aatctgttcc
     4621 aattccatcg ccagtttgaa tttcggactg tcttccaagc ccaattcctt caaaacttca
     4681 tagcaggttt cgcgcataat gctggcacgc ggatccatat tgcggtacac gcggtgaccg
     4741 aagcccatca gacggtattt gcgttgtttc acaccttcca tgtatgcggc aacattagac
     4801 acatcgccga tttcgtccaa cattttcaac acggcttcgt tcgcaccgcc gtgcgaagca
     4861 ccccacaggc aggcgatacc ggcagcaata caggcaaacg gattcgcacc cgaagagcct
     4921 gccagacgga cggttgaagt tgaggcgttt tgctcgtggt cggcatgcaa aataaagatg
     4981 cggtcgagcg cgcgtgccaa aacgggattg ggtttgtagt cttcacacgg cgtggcgaac
     5041 atcatatgaa ggaagttttc ggaataagaa agattattct tcggataatt gaacggcaga
     5101 ccgtttgaat agcggtagca cattgccgca atggtcggga ttttggaaat caggcggtaa
     5161 atcgcgattt tgcggtgttc gggattgcta atgtccaagc tgtcttggta gaacgcagac
     5221 agcgcgccga ccacgccgac catcatcgcc atcggatgcg cgtcgcggcg gaacccccgg
     5281 aagaaccaag tcagctgttc atgcaccatc gtgtggcggc ggacggtatt gtcaaattct
     5341 gccttttgct cgggagtcgg cagttcgccg taaatcaaca ggtagcagac ttccaaataa
     5401 tcggattttt cggccagctg ctcgatgggg tatccgcgat aataaagcaa gccttgatcg
     5461 ccgtcgatgt aagtaatttt agactcacag cttgcggttg aaacaaatcc ggggtcgaag
     5521 gaaaacaaac ctgtattttt tgtcagcccc cgaatgtcaa ccacatcgtg cccgatgctg
     5581 gcttccaata ccggcagctc caaacctgcc tgacccggta cgttgagttt gattgatttg
     5641 gacatattat tgctcctttg caagttataa cccacgttac tgcatccgca gcacgttctt
     5701 tgccttcaga cggcttttgc attttgaaat ctgcctgcag gcagatttca gatgcctcat
     5761 gcccgtctga ttttttcaag catcgggata aggtgccctt tgtccgtttc cgaatgcccg
     5821 ttaatcaagg caagcaattc ttgatcttga aattcaagga tttcggaaaa ctcggacagc
     5881 tctttatcgc tcaaatgctc gaattctttt tccataaacc tgccgaagat taaatctaac
     5941 tccaacaatc cccggcgggt ttgaaaacgg attttccgtt tggcaatatc gtcaaaaacc
     6001 atcatttctt aaacggcccg cttcaacata atctctttaa tcttaccgat ggctcgggtc
     6061 ggattcaagt gtttggggca tacgtccaca cagttcataa tggtgtggca gcggaacaaa
     6121 cggtatgggt cgttcagatc atccaaacgc tcattagtga tggtatcacg gctgtccgca
     6181 atgaaacggt aagcattcag caaaccggac ggaccgacga atttatcagg gttccaccaa
     6241 aatgacgggc aggcagtcga acagcaggcg cacaaaatac actcgtacaa accgtccaac
     6301 tctttacgct cttcctgagt ttgcagacgc tctttgtccg catcaatcgg attatcgttg
     6361 acaacataag gtttgacgga atggtattgt ttgaagaact gggtcatatc cacaatcagg
     6421 tcgcggataa caggcagacc tggcagagga cgaattttaa ccggctgttt caagccacgc
     6481 agatcggtca gacacgccaa accgtttttg ccgttgatgt tcataccgtc cgatccgcaa
     6541 atgccttcgc ggcaggaacg gcggaaagac aatgtatcat cttgtgcttt caggcgcact
     6601 aaagcgtcca aaagtttaac gtcggttggt tccaattcca actcgtaacg ctgcatataa
     6661 ggtttggcat caacgtccgg gttgtaacgg taaatttcaa aactcatttt ttccatgaga
     6721 agtgttcctt ttctcataaa ctgcccaatg attttgtatt atgaattcaa gcagttcaat
     6781 gtgggtatgg ttagatttca gacgaccttt gaggcgaaga ccgtctgaaa acgcatcaat
     6841 aaacgcgctt ggccggtttg atgtattcca cgctcaaggg cttggtatgc accggtttgt
     6901 aagacaaggt attggtatct gaatggtaca gcgtatgttt catccagttt tcatcatcgc
     6961 gctcaggatg gtcgtctgaa gcgtgcgcac cgcgtgattc tttacgtgct tcggcagaca
     7021 ccaaagtcgc tttcgccact tcaatcaggt tatccaattc caaagcctcg atacgcgcgg
     7081 tattccacac tttgctcttg tctttgattt cggtacgttt cacacgctcg gcaatcgcca
     7141 tgacttctcg aacgcctttg ctcagaatct catcagtacg gaacacgccg gcgtgcagtt
     7201 gtacagagcg ttgcagttcg cgacgcaatg catcaacgtt ttcaccatcg gtttggttgt
     7261 ccaaacgctc gatacgttgg cgggtcaact cacctgcatt agcaggcaaa ggtttccagt
     7321 cgctttgctc tttgatgaat ttaatcatgc tgtcaccggc agctttaccg aataccacca
     7381 agtccaacag ggagttggta cccaagcggt tcgcaccgtg tacggaagca caagcgcact
     7441 cacctgccgc atacagacct tttacaggca cttcgtaatc ttcaccttgc ggaacgacaa
     7501 cttcgccgtg gtaattggtc ggaatgccgc ccatcatata gtgggtagtc ggcacaacgg
     7561 gaatcgggtc tttaatcgga tcgataccgg cgaactgaat ggaaatctcg cggatgcccg
     7621 gcagtttttc cataattttt tctgcgccga tatggtcgat tttcagtaag acatggtctt
     7681 tgtttttacc gcagccgcga ccttcgtaga tttccatcgc catcgcgcgg gaaacaacgt
     7741 cgcgagaagc caagtctttt acggtcggcg catagcgttc cataaagcgt tcgccgtcgg
     7801 cattcaacag aataccgccc tcgccgcgta cgccttcggt aatcaacacg cccgcacccg
     7861 ccacgccggt cgggtggaat tgccagaatt ccatgtcttc caacgggata cctgcacgcg
     7921 cacaaatacc caaaccatcg ccggtattca tataggcatt ggtagaagac gcataaatac
     7981 gaccgccgcc gccggtagca aacatcacag ctttagcgtg gaaaatataa acttcgccgg
     8041 tttccatttc catggcggtt acgccgacga catcgccgtt ttcatcacga atcaaatctt
     8101 gtgccgtcca ttccacaaag aattgcgtat tggcacggac gttttgttgg tacaaagtat
     8161 gcagcatcgc atgacctgta cggtcggcaa ccgcacaggc gcgttctacc gcgcgtttac
     8221 cgtgttcggc agtatggccg ccgaaaggac gctgataaat tttaccgctt tccacacggt
     8281 caaaaggcat acccatgtgt tccaactcaa ttacggcttc aggcgcggcg cggcacataa
     8341 actcaatcgc atcttggtcg cccaaccagt cggaaccttt cacggtatcg tacatgtgcc
     8401 agtcccaacg gtcttcctgc acattaccca gagaggcgga aataccgccc tgcgccgcta
     8461 cggtatgcga acgggtcggg aacactttag acaaaacggc acaattcaga ccggatttgg
     8521 ataattggag ggctgcgcgt aaacctgcac caccaccgcc gacaatcacg gcatcaaact
     8581 tgcgaacagg aaaacccata cttaccccca aatcacttta actgaataca cgagacagcc
     8641 gaccagccaa acgatggtgg caacctgcaa aaacaaacgc acgccgaagg gtttgatata
     8701 gtccatccac aaatcgcgga tacccaccca agcgtgcaag aatacggcga tgaagctcac
     8761 ttgggtaaat acttttaccc aagtttgact aaaaaatgcc tgccatgccg aatattcttt
     8821 aggcagggaa aatagaacca ctaaaagtgc aacggtataa atcaacataa taaccgcagt
     8881 cgcacgttgc atcacccaat cgcgcaaacc gtaatgggca ccggtcaatt tacgttctac
     8941 cataacaacg ctcccaaaac gacagtcaaa accaatgcag aagcaaatac ggctttagcg
     9001 gtattgcgcg cagtattcag ctcaaggcct ttgtgcgcat ccaaaaataa aaagcggata
     9061 ccggcgagag aatggtgcag ataagcccac aatacaccga ttaaaaccag cttgaccaaa
     9121 ggatgggaaa caatggcacg gtaagtttca aatgcagact cttgactcag ggtaccggac
     9181 aggaaataca gcaggaaagg cagcataata aacagcccga ccccgctgat gcggtgaagg
     9241 atggaaacta tcccgggtat cggcagacgg atgttcggca aatccagata aacaggacgc
     9301 ggtttggcag acatagtcaa ttcctctgta gttatatatc gactcaaaaa ccgtaaaccc
     9361 attgctacac tgggttacat aaacgttaat ttacctgttt tatcccattt tgaacagtgt
     9421 attttaaaaa caaactgata gattttgaca aacacgcgtg gaaacagcag attaaaagaa
     9481 agatttttag ttttgccgcc cccttcccga cacggtatca gcatccgacc ccatccccaa
     9541 aaacaaaatg ccgtctgaaa aaatttcaga cggcattgtt ttgccatatc ggtcaggctt
     9601 tcaatttcgc atcggcatcg cgcaacgcgg tacgcagacc ttcctcgata acgggatggt
     9661 agaacggcat atccagcatt tgcgaaacgg tcatcttcat ttgatgtgcc caagccaaca
     9721 ggtgcgccaa atgttcggcg gcagggccta cgatttccgc gccgataaag cggccggtgg
     9781 ctttttcggc atacaggcgc atatggcctt tgttcaccag catcacgcgg ctgcggcctt
     9841 ggtttttgaa cgatacttcg ccgatgacaa attcgtcggc ttggtattgc gcggcaacct
     9901 gcgcgtattt cagaccgaca aagccgattt gcggactggt aaacaccacg ccgatggtgc
     9961 tgcgccgcaa accgccgccg atattcgggt agcggcccgc gttatcgccg gcaatcttgc
    10021 cttggtcggc agcttcatgc agcagaggca gttggttgga cgcatcgcct gcgatgaaga
    10081 tatgcggaat actggtctgc atggtcagcg ggtcggcaac aggtacgccg cgcgcatctt
    10141 tttcgatatt gatattttcc aaaccgatat tgtcaacgtt cggacggcgg cccacggctg
    10201 ccagcatata ttcggcaaca aatacgcctt tttcgccatc ctgctcccaa tggacttcta
    10261 cattgccgtc tgcatcgagt ttgacctcgg ttttagcatc cagatgcagt ttcaattctt
    10321 cgccgaacac ggcgttcgcc tcgtctgaaa cgacggggtc ggaaatgccg ccgatgattc
    10381 cgcccaaacc gaaaatttca actttcacgc ccaaacggtg caatgcctga cccagttcca
    10441 aaccgataac acccggcccg aacacggcaa cgcgcttagg cagcgtatcc catgagaaaa
    10501 cgtcatcgtt gataatcaaa cgattgccca aagactgcca ttgcggcagg atgacgggac
    10561 gcgaaccggt agcaatcacg aaactttttg ccgtaatttg agtatgctcg tcgatttgga
    10621 cggtatgctc gtcgataaat ttagccgaac ccataatgcg cttgtcggca ggccactctt
    10681 ccacatcggc aacgacaaag ccgacaaaac ggtcacgctc ggatttaacg cgctgcatga
    10741 cctcttcacc gttgacgacg atgctgtctt tgtccaaatg cacgccgaac gggtcggtat
    10801 gcaatgcgtg atgacgcgcc tctgcggcgg caatcaagag tttggaaggc atacagccca
    10861 cgcgcgcgca ggtcgtgccg aacacattgt tttcaatcag gtaaacatta tccgaatgta
    10921 aacgggcatt gcgaaacgca cccataccgg cagtaccgcc gccgattacg acgacatccg
    10981 cttgaatttt tttcataatc tttgtccttc cgttttcgtc tgaaacattg ccgacacaga
    11041 acatatcggt ttcagacggc atttaaatat tttcccaatc aaaaacatca ttcagataaa
    11101 taaaaacaaa atttcgattg agtgcaacaa tatccaaatt ctcttataaa tatagagtta
    11161 ttcggagcgg tagtgtagaa ccgcccgaat aactctatcc ggtatatcag acagtttaat
    11221 taaaccgcct taagcaaaca gctatcagtt tttagccaag taagcttcca aatcttcgct
    11281 gccgccgatg tatttaccgc cgatgaagac ttgaggggca gtcatcttgc cggtaatggc
    11341 gcgaacggaa gtgacggttg catctttgcc caatacgatt tcttcgtaag acaaaccttt
    11401 gtcttgcaaa gcttgtttgg ctttagcgca gaattggcaa cctggtttag tgaaaattgc
    11461 cacagactct tgagccttcc aatcgggagc aacgaattgc agcatagtat ctgcatcgga
    11521 tactttgaac ggatcgcccg gttcttcagg ttcgatgaac attttttcaa ccacgccgtc
    11581 gttaaccagc atggagtaac gccaagagcg tttaccgaag cccaagtctt ctttaccgac
    11641 cagcataccc ataccttcgg taaattcgcc gttgccgtca ggaatcatgt agatgttgtc
    11701 ggattcttct tcggcagccc aagcgttcat tacgaacgta tcgtttacag atacgcagta
    11761 gattgcgtca acgccgtttt ctttgaacgc gccgaacaat tcgttgtaac gcggcaggtg
    11821 tgaagaagaa caagtcgggg taaatgcacc gggcagggag aatacgacta cttttttgcc
    11881 tttgaacaaa tcatcggtag acacatcttt ccaagtgtcg ccgacgcggg tgcggaatac
    11941 tacggaaggt actttttgac cggtacgatc ttgcaaagcc attttaagct ccttaataga
    12001 ttggtttgag aaataattgt gaatcgctgg gcattctacc ccatgcaaaa tgatttgtat
    12061 aatttatagt tcaaataata taaattattt aaaactatca aacacaactc cgatttgcct
    12121 gtttcccaca tataaagccg aattagaaaa aaacaagctg caatgaaaag attttccggc
    12181 aactgcgccc ttgcgccgca aatcaggaca aaataccgtc tgaaaggctt tcagacggta
    12241 tttattcata cggtctcggc ttatttcgcc aattcggcac gcagttttcg ggttacgttc
    12301 atcattactt ggagttgttc cagagtttct ttccagccgc gtgttttcag gccgcagtcc
    12361 gggttaaccc acagacgttc aaccggtaca acctcgatgg ctttgcgcaa caggtgctcc
    12421 acttcggctt ctgtcggtac gcgcgggctg tggatgtcgt aaacccccgg gccgatgtcg
    12481 ttcgggtatt ggaattcgcc gaacgcggtc aagagttcca tgtcggaacg tgaagtctcg
    12541 atggtgatca cgtccgcatc cattgcagca atcgcaggca ggatatcgtt gaactcggag
    12601 taacacatat gagtgtggat ttgggtgctg tcttcgcaac cggcagagga caggcggaag
    12661 gattcgcccg cccagttcag gtaggcatcc caatcggcgc gtttcagcgg caagccttcg
    12721 cggatggcag gttcgtcaat ttggatgact ttgatgcctg ctttttccag atccaatact
    12781 tcgtcgttca gagccagtgc gatttgtttg cacacggtag agcgaggaat atcgttgcgg
    12841 acaaaagacc attgcagaat ggttacaggg ccggtcaaca tacctttcat cgggcgtttg
    12901 gtcaggcttt gtgcgtaagt agaccaggcg acagtcatgg cttcaggacg gcttacgtca
    12961 ccgaagataa tcggtggttt aacacaacgt gagccgtagc tttgtaccca gccgtattgg
    13021 gtgaatgcaa aaccgctcaa caattcgccg aagtattcga ccatgtcgtt acgctcggct
    13081 tcgccgtgta ccagtacgtc caaatccagt ttttcttgtt cttcaaccac caaggcgatt
    13141 tcttttttca tcgcggcttc gtaatcggcg gcagacagtt cgcctttttt gaaggctgcg
    13201 cgtgcttggc ggatttcggt ggtttgcggg aaagaaccga tgttggtcgt cggcagcaga
    13261 ggcaggttca accatgcttg ttgcgctttg atacggtcgg caaatggaga tttgcgttgg
    13321 tctgcgttgg caggcaaatc ggccaggcgt ttggcaacgt ctgcacggtg gatttcgctg
    13381 ctgttggcgc gtgaatcggc agcagcttgg ctggcggcca gttcttcggc aacggaatcg
    13441 cgaccttcgt tcaatgcggc tttcagaacg cgcaattctt gggttttttg cagggtaaat
    13501 gccaaccaag agtacaggtc cggtttgttg gctttcagtt tttcttcaac tgacaagtca
    13561 aatggagtgt gcagcagaga gcaagagctg gaaatccaca aacggtcgcc cagtttggct
    13621 tgcagaggct caacagtttc caaaactttg ttcaggttgg cgcgccagat gttgcggccg
    13681 tcgatgatgc ctgcagacag aactttgtcg tagtcggcaa atgcgtccag ttgctcggga
    13741 gcgcgtacca agtcgatgtg caggccgtca acaggcaggg atttcaacaa tgcggcgtgt
    13801 tcggcaacag aaccgaagta agtgctcagc aggattttgg cacttacttt gctcaaagta
    13861 gcgtaaacgt ctttgtaggc ttctacccat tctttaggca gatcgacagt caaagcaggc
    13921 tcgtcgattt gaatccactc ggcaccggct tcaaccaaag cagtcaggat ttcaacgtaa
    13981 acaggcaaca gtttaggtaa caggctcaga cggtcgaatt caacggcgcc tttttcttta
    14041 ccaacccaca ggaaagtcaa cggacccaca acggtcggtt tggctttcaa gcccaaagct
    14101 tgggcttctt gcagttgttg gacgtagtgt ttggcgttgg ctttgaattc agtattggcg
    14161 tggaattcag gcaccaagta gtggtagttg gtgtcgaacc atttggtcat ttcgatagcg
    14221 aattggtctt tgttaccgcg cgccagttgg aagaattgtt ccaaagacag gttttggctg
    14281 tcgaagccga agcgggcggg aatcgcgccg gtggcgactt gcaggtcgag gatgtggtcg
    14341 tagaaagtga aatcgcctgc ggcaacgtaa tcggcgttgg cagcagcttg gtgtttccag
    14401 tttttctcgc gcaagtcttt agcaacagcc agcaattctt gctcgctgat ttctttgcgc
    14461 cagtattttt cttgggcgaa tttcaattcg cggaaggcgc cgacacgcgg gaagcctgag
    14521 aaatgtaatg ttgtcatgtt gaactctcca gttggatttt gtaaggaccg gttcaatccg
    14581 aatcggccgg tattgtaaat cagaatggct cggatggggc ggtgttttgc tttttagaca
    14641 ccttagatgc cgtctgaaac tgtttgatac ggctttaagg gcgcacgcct aaaatatggc
    14701 aaatggcgta agtcagctcg ctgcggttaa gcgtatagaa gtggaaatct ttcacgcctt
    14761 cgcgggacag gactttgacc atatcgatgg cgatacttgc cgccaccaga ttgcgcgtac
    14821 cttggtcgtc gtccaaacct tcatacattt gcgacagcca gcttgggatt ttgacgttgg
    14881 ttacttgagc cattttaccg agctgcttga agttggtaac aggcaaaata ccggggacga
    14941 tttccacatc gatacccaac atcacgcagc ggtcgcggaa gcgcaggtag cgttccacat
    15001 cgaagaagaa ttgggtgatg acgtggttcg cgcccgcatc gattttgcgt ttcaaattaa
    15061 tcaggtcggc ttgtgcggat ttcgcttcgg gatgcacttc gggatatgct gctacagaga
    15121 tgtcgaagtc ggcgacggag cgtaatagct taaccaagtc ttcggcgtaa aacggttttt
    15181 tctcataacc ggccggctcg tctccgcgca gggcgacaat gcggcggatg ccgctgtccc
    15241 aataatcttt ggcaatttgg cgcaattcgt cgggagaagc gtcgataccg gtcaggtgag
    15301 gcgcggcttc caagccggtt tcctgtttga tgcgtttgac gatgccgtgt gtgcggtcgc
    15361 gctcgcctga gtttgcaccg taagttacgg aaacaaattt cgggtgcaag gtttgcaggc
    15421 gatggatgga atcccacagc atggtttcca tttgttcgtt tttcggcggg aaaaattcga
    15481 atgaaacgtt gatgtcgcct ttcaaatcgg aaaggctgtt atttaacgca ttgatttctt
    15541 ttgcgtaatt catggctgcg taccttttat tatcttttat atgatgtttc aggcaacata
    15601 ataaagttgc gccaaacatg agtcaatttc aaaattttca gatttatcat gaaaatttct
    15661 aatatattac aaaaatagtt aaatttcctt gtacagccct tctaaactat gctagagttt
    15721 gttatgttat aacactgaaa gcaaggcaat atatgaaacc gaatattcat cccgacaatt
    15781 accgaaccgt cctttttttc gatagcagcg ccaatgaagg ctggttaatc cgctcctgtg
    15841 ccgaaacaca cggaaaaacg atggtttgga cagacggcaa agaatatccg ctgttttcgc
    15901 tggatacttc ttccgcatcc catcccgtct atactggcaa acagcgcaac gtcaacaccg
    15961 aaggccgtgc cagcaaattc aaccaacgtt tccaatccgt aatgtcatcg tttagaaagg
    16021 acaaataatg caggttctgt cttcactcaa aaccgccaaa caacgccacc gcgactgcca
    16081 aatcgtcagg cgcaggggca aagtttacgt catctgcaaa agcaatccgc gttttaaagc
    16141 gcgccaacgc tgaccccctg cccttcctgc ttcggttgta aatcccctcc taccgcaagc
    16201 ccttttcaaa aaatgccgtc tgaaacagaa aaatgcgttt cagacggcat tttttccgtc
    16261 aatacgaatc cggaatgtgt ttgaaataca gcataaacag ataccaaaac agcgtcgcgg
    16321 cggccgcacc gagcagcacc cgggcactga tttcggcaac ttcgcgcagg gcgcgtttct
    16381 gcctgcggct gaagaggcgg ttcataatca gcgcggcaag tgcgaaaaag aaaaaaatac
    16441 ggaaaagtct gcctatcatg tcgggaagcg aaaagtatgg ttcgggcgcg gcggcatggg
    16501 gaaggatgtt tcagcgcgtc aggctgcttg tccgaccgtg tcaaaaagtc atgccgtttg
    16561 cgatataata acgctttttg ccgtatttgt tggaacagga tatgagtcaa aatgcctcgg
    16621 tgggcattgt aatgccccaa aaaattccgt ttgaggtgcc gctggttttg gaaaacggta
    16681 aaactttgcc gcgtttcgat ctgatgattg aaacctacgg cgagctgaat gctgaaaaaa
    16741 acaatgcggt tttaatctgc cacgcgctgt cgggcaacca tcatgttgcg ggcaggcatt
    16801 cggcggaaga taaatatacg ggctggtggg acaatatggt cggacctggc aaaccgattg
    16861 atacggaacg ttttttcgtg gtcggtttga ataatctggg cggctgcgac ggcagcagcg
    16921 ggcctttgtc gatcaatcct gaaacgggta gggaatacgg cgcggatttt ccgctggtta
    16981 cggtgaagga ctgggtaaaa tcccaagccg cgcttgccga ttatctcagc atcgggcaat
    17041 gggcggcggt tgtcggcggc agcttgggcg gcatgcaggc tttgcagtgg gcgatttcct
    17101 atcccgaacg cgtgcgccac gccttggtga ttgcgtccgc gccgaaactg tccacgcaaa
    17161 atatcgcgtt caacgatgtg gcgcgtcagg cgattttgac cgatcccgat ttcaacgaag
    17221 gacattaccg cagccgcaac accgttcccg ctcggggctt gcggattgcc cgcatgatgg
    17281 ggcacatcac ctatcttgcc gaagacggtt tgggcaaaaa attcggacgc gatttgcgtt
    17341 ccaacggcta tcaatacggc tatggcgttg aatttgaagt agaatcctat ctgcgctatc
    17401 aaggcgacaa attcgtcggg cggtttgatg ccaacaccta tttgctgatg accaaggctt
    17461 tggactattt cgatccggcg gcagatttcg gcgacagcct gacccgcgcc ctgcaaaatg
    17521 tgaaagcgaa attctttgtc gccagcttca gcaccgactg gcgtttcgca cccgaacgtt
    17581 cgcacgaact ggtcaaggca ctgattgccg cccaaaaatc cgtgcagtat atcgaagtta
    17641 aatccgcaca cgggcacgat gcctttttaa tggaagacga agcctatatg cgtgcggtcg
    17701 ccgcctatat gaacaacgtt tataagggat gtcagcaatg aacctgcgcg atgatttgca
    17761 actgatttac gaccggatac ccgaaggcag ccgcgtcttg gacttgggct gcggcgacgg
    17821 cgaactgctt gccgcattgg tcgaacacaa aaaatgcagc ggctacggca tcgaaatcga
    17881 caccgacagc gtgattgccg cgatgtcgcg cggcgtgaat gttatccaag ccgatttgga
    17941 agaaggttta accgcattca acgatcaaag ttttgatgtg attgtgttga gccaaaccat
    18001 ccaagcgatg cagaataccg aaaaaatcct gcgctgcctg atgcgcgttg ccaaacaggc
    18061 gattgtcagc ttcccgaatt tcggctactg gcgcaaccgt gtccaaatcg cactcggcgg
    18121 acatatgccg gtttccgaac gtatgcccta ccattggtac gacacgccca atatccattg
    18181 gtgcacgctc aaagactttg atttgttgtg cgccaaaaac aacatccgcg tcctcgaacg
    18241 cgccgtgatg acgggcaaca gacaggtcaa acatttcccc aaccttttgg gcagcctggc
    18301 gttttaccgc gtcgggtaag acaggttgag aaccttgtcg gcaataacag gttcttcggt
    18361 tctattaaca acaggccgtc tgaaaaatga aacggcaggt tttcagacgg cctttttaat
    18421 attggaaaca atagaatgaa aaacaaaacc tcatcacttc tcttatggct tgccgcaatc
    18481 atgctgaccg cgtgttcccc gaacaaagaa gataaaacca aagaaaccgg cgcatctgcc
    18541 gcttcgtccc cggcatccgc cgcttcgtct tccgcgcccc aaaccgattt gcaaccggcc
    18601 gcatccgccc ctgataacgt caagcaggca gaaagcgcgc cgccgtcaaa ttgcaccgac
    18661 ctgcaccccg ccaccggcat tgacgatctc atgcagcaaa tcgccgaaca catcgactcg
    18721 gactgtctgt ttgccctttc ccatcacgaa ctggaaaccc gtttcggctt acccgacagt
    18781 ggctatgaca acatacagcg gctgctgttt cccgacatcc gccctgaaga tcccgactac
    18841 catcagaaaa tcatactggc aattgaagac ttgcgttacg gaaagcgcac gatcagccgg
    18901 caggcacaag atgccttgat ggaacaggaa cgccgcctcc gagaagcgac gctgttgctg
    18961 acacagggca gcgaagaaac ccgcgggcaa ggcgaggagc cgaaacgcac gcgttatttt
    19021 gaagtttcgg caacccctgc ctattcgagc cggcacaaca acggacttgg cggcaatttc
    19081 caatacatcg gccaattgcc cggctatctg aaaatacacg gagaaatgct tgaaaaccaa
    19141 tcactcttcc ggctgtccaa ccgtgaacgc aatcccgaca aaccgttttt agacatccat
    19201 tttgacgaaa atggcaaaat cacgcgtatt gtcgtttacg aaaaaaacat ctacttcaat
    19261 ccaaacttgg ggcgaagata aaaaaatgcc gtctgaaagc ctttcagacg gcatttttca
    19321 agtaggatat caggcgcgtt tgcggaattc gccggtgcgg gtatcgattt cgactttgtc
    19381 gccgttttcg atgtaagaca tcacttggat ttcggtgccg ccgaccagac gcgcggtttt
    19441 catcactttg ccggaagtgt cgcctttgac agcaggctcg gtgtactcga cttcgcgtac
    19501 gatgatggta ggcagttcga cagaaatggg gttgccttcg tagaaggtaa cttcgcattg
    19561 gtcttccata ccgtcaacga tgaatttcaa cgcgtcgccg atgttgtcgg cttcgatttc
    19621 gtattggttg aattcttcgt ccataaagac gtacatcggg tcggcaaaat agctgtacgt
    19681 acagtttttg cgggacagga tgaccacgtc gaatttgtcg tcggctttgt aaatggtttc
    19741 ggaagccgcg ccggtcagca ggtttttcag tttcatgctg actttggcgg aagagcggcc
    19801 gcctttgatg tattcggttt tttgaacgac cataggatcg ttgccgacca taaatacatt
    19861 gccggcgcgc agttcttgtg ctgttttcat tgtgattttc caatcaaatc ggttgtcgta
    19921 aaacgcgcta ttctagcgta ttttttgatg ctttgaaaca aaggcggcga gtttttcgga
    19981 tgcggaaggc tgcccgaaaa gataacggct ccaatcctcc gcgccttgcc gccagccgtt
    20041 ttgatgttgt tgcagggttt gccaacattc gaggcgttgc gtggcggata aagcctctcc
    20101 gccgttgagg tcgtctgaaa ggcggcggtg taccgatgcg gtttcgggcg agtaaaatcc
    20161 atgcgcctta tcccaaaagg cgtggagttt atcgagatgg acattctcgt cttgcgggta
    20221 gatgtgccaa aagaagggtt tgccggcaag ctgggcgcgc acgaaactgt cttcgccgcg
    20281 tatcacggcg cagtcggcga ggtgcagcag tttgtcgaag tcctgttgcg gcacgaaagg
    20341 gattttgacg aggcggacgg atgccgtctg aaaaacatcg ccgtcgtttt gcagggcatc
    20401 ttgcggaata acgccgcttt gtttgaggct gtcgatgatt tgcgtccccg ccagcaacag
    20461 tgtcatcggg ctgcctgcct gtcgccacat ttccagccac tttgcccaaa catcgctccg
    20521 atagccgaaa agcagccatt cgggggcgtt tttttcggga agcatcagcc gctctcgcaa
    20581 ggcttcggta tcgaaacgga cggcatttcg gtaatcgcgt tcgcgtatca acccgccgct
    20641 tttttcgctg aaacccataa accaaaaata tttttgaaca ctctcctgca gcgaaggcat
    20701 cagatgcagc ctttcattgc tttcttccgc gctcaaatat tcccaattca gccaaagcgg
    20761 cttgtgtcgg cggatgatgt gcagcacatt ttcgggcagg tcgcaggcaa aagtttcgat
    20821 aacgatatcg ggaacaaggg cagtatcgat atccgccgca tcggaatgcc aagtgcggac
    20881 atgaatatcc tgatgaacgc agggaacatc gggcaaatca gggcaaagcg cacgcaaggc
    20941 ggacacatcg tccgtccaca aatgcacctg ccaaccgagt tcgcggtgca aaacacgggc
    21001 gagccgccac gaaacgccga tgtcgccgaa attgtcgatg accttgcaaa aaatccaaca
    21061 gacaaaagga ggagtattca tagaaataac ggaaacaagc taaaacgcgc cgaaatagtg
    21121 gaacaaatac acaatcagca aaataatcgg aataaacagc aacatagttt gacgtttcaa
    21181 atcaaactct tgtttcaaat gatgcttgat ggtgtcgaaa aggacgaatt taaacaaaaa
    21241 cgtcgcttcg caggcaagga tgatgccgtt gatcagatgc tggagtttgt cttcgggcgg
    21301 attggcggcg aaaacggcaa gcgtcgccaa cgggatgagg cagacggcaa gccaacggac
    21361 aagcaggttt cgagacattg caaggctttc aaaaaaagca aaacgccatt atcgccccaa
    21421 gcgcacgcct tgtctaacgg acgcaccgga gatgccgtct gaagccttgc cgcaggaaaa
    21481 caccgcaccg ccgaaccttt aaatacgccc gagacgggcg ttccccgaaa aatcggacgc
    21541 tgccgccgat gcctgaggac aaggcggcag caggcacggg catattgaaa acccggcgcg
    21601 tcggtttgaa
//
LOCUS       DAWGJV010000016        21337 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWGJV010000016 DAWGJV010000000
VERSION     DAWGJV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.016"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(83..774)
                     /locus_tag="WHQ94_000433"
                     /pseudo
     CDS             complement(83..774)
                     /locus_tag="WHQ94_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1080..1637)
                     /locus_tag="WHQ94_000434"
     CDS             complement(1080..1637)
                     /locus_tag="WHQ94_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127771.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1881..2039)
                     /locus_tag="WHQ94_000435"
     CDS             complement(1881..2039)
                     /locus_tag="WHQ94_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127772.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2036..2878)
                     /locus_tag="WHQ94_000436"
     CDS             complement(2036..2878)
                     /locus_tag="WHQ94_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2127773.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3113..4033
                     /gene="pyrB"
                     /locus_tag="WHQ94_000437"
     CDS             3113..4033
                     /gene="pyrB"
                     /locus_tag="WHQ94_000437"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ2127774.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4043..4501
                     /locus_tag="WHQ94_000438"
     CDS             4043..4501
                     /locus_tag="WHQ94_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ2127775.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4566..5231
                     /locus_tag="WHQ94_000439"
     CDS             4566..5231
                     /locus_tag="WHQ94_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127776.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5294..6511)
                     /locus_tag="WHQ94_000440"
     CDS             complement(5294..6511)
                     /locus_tag="WHQ94_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ2127777.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAGE
                     RLKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6653..7198
                     /gene="def"
                     /locus_tag="WHQ94_000441"
     CDS             6653..7198
                     /gene="def"
                     /locus_tag="WHQ94_000441"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ2127778.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7285..8211
                     /locus_tag="WHQ94_000442"
     CDS             7285..8211
                     /locus_tag="WHQ94_000442"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ2127779.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8290..9549
                     /gene="rsmB"
                     /locus_tag="WHQ94_000443"
     CDS             8290..9549
                     /gene="rsmB"
                     /locus_tag="WHQ94_000443"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ2127780.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLGALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9515..10114
                     /locus_tag="WHQ94_000444"
     CDS             9515..10114
                     /locus_tag="WHQ94_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ2127781.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10114..12234
                     /locus_tag="WHQ94_000445"
     CDS             10114..12234
                     /locus_tag="WHQ94_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ2127782.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRKREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12227..13504
                     /locus_tag="WHQ94_000446"
     CDS             12227..13504
                     /locus_tag="WHQ94_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ2127783.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEVGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13595..14782
                     /gene="dprA"
                     /locus_tag="WHQ94_000447"
     CDS             13595..14782
                     /gene="dprA"
                     /locus_tag="WHQ94_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ2127784.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14808..15269
                     /locus_tag="WHQ94_000448"
     CDS             14808..15269
                     /locus_tag="WHQ94_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ2127785.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15341..17647
                     /gene="topA"
                     /locus_tag="WHQ94_000449"
     CDS             15341..17647
                     /gene="topA"
                     /locus_tag="WHQ94_000449"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ2127786.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAETCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17725..17991)
                     /locus_tag="WHQ94_000450"
     CDS             complement(17725..17991)
                     /locus_tag="WHQ94_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEZ2127787.1"
                     /translation="MASSEPFPCSACGLCCQKVNLSEQTIYLDRGDGTCRHFDDSTRL
                     CTIYETRPLVCRVEDYYHHYLSEQMSWQDFIQINIEVCKQFQSE"
     gene            complement(17991..18410)
                     /locus_tag="WHQ94_000451"
     CDS             complement(17991..18410)
                     /locus_tag="WHQ94_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127788.1"
                     /translation="MGKIQILNKLPTKNPATIPFDAASNSFFRILEARNEYKKIAAQE
                     KTKREVIGAWRDVRLAELKNQQEILRLYLENTFKERRKMIDGLFEALDKGMEAGNIHV
                     IDTAINGIVSIAKDSPLKQVDNLIAALKNDNVKVIDF"
     gene            complement(18410..19357)
                     /locus_tag="WHQ94_000452"
     CDS             complement(18410..19357)
                     /locus_tag="WHQ94_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127789.1"
                     /translation="MAIPVLLWGAAAALAATGIVKGAAALSDIDDAKEIGERAERRYK
                     RRKRELNSTKDETNLAFEELGQFKVDIFNNQIRHIVQVLNNTSNKHAKSELSGFNSTF
                     DIAELKEMETMVQTSLELSTNLGSSAITGALAGFGVYGAVPMIATASTGTAISTLSGA
                     AATNATLAWLGGGSLAAGGFGMAGGMVALGGIVAGPALAIGGFMLASKAEEALTEARA
                     YSAEIDEAIDEMDLITEGLEGLKENAREIRHTLEEMVKRFEKVRVDHADHPDFHTMLV
                     IGKAIKNLLNTPIMEEDGSPVANIRHQCEGCLNIELGTV"
     gene            19514..19891
                     /locus_tag="WHQ94_000453"
     CDS             19514..19891
                     /locus_tag="WHQ94_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2127790.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19980..20549
                     /gene="rsmD"
                     /locus_tag="WHQ94_000454"
     CDS             19980..20549
                     /gene="rsmD"
                     /locus_tag="WHQ94_000454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ2127791.1"
                     /translation="MAAGKHTKHSNQVRIIGGQCRGRKLSFASANGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20672..20923
                     /locus_tag="WHQ94_000455"
     CDS             20672..20923
                     /locus_tag="WHQ94_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ2127792.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21020..21103
                     /locus_tag="WHQ94_000456"
     tRNA            21020..21103
                     /locus_tag="WHQ94_000456"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21054..21056,aa:Tyr,seq:gta)
     gene            21134..21207
                     /locus_tag="WHQ94_000457"
     tRNA            21134..21207
                     /locus_tag="WHQ94_000457"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21166..21168,aa:Gly,seq:tcc)
     gene            21217..21291
                     /locus_tag="WHQ94_000458"
     tRNA            21217..21291
                     /locus_tag="WHQ94_000458"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21249..21251,aa:Thr,seq:ggt)
BASE COUNT         5306 a         5472 c         5770 g         4789 t
ORIGIN      
        1 ggtttcgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgatta ctatataaaa
       61 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
      121 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      181 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      241 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      301 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      361 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      421 caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc atttgggttt
      481 tatccatttt gtccaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      541 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      601 ctcgccgtgc tttccaactt cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      661 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      721 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      781 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      841 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      901 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      961 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
     1021 agttattaat gattatttcc gagaattgac tgcattgttg gcagcattgg ccccaaaacc
     1081 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1141 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgccgc agtagccgcg
     1201 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata ctcccatgca
     1261 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1321 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1381 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1441 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1501 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1561 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1621 ttgtgttttt gcatcatttc gtttgatttt ctagtttttg agaatgatac aaagtttttt
     1681 acaaagtaaa gagtcactct gaaaaacatt ttttcattat aaatcaaaat attgatagaa
     1741 taaatagcga gcatcgattc acggtgtact ttagtgcaaa gcgtacaacg cgagcctgaa
     1801 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1861 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1921 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1981 ccggaaaggg cgcaggcggc gagtgccgac agggtgcaga tggggagggg ggttttcatt
     2041 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2101 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2161 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2221 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2281 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2341 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2401 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2461 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2521 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2581 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2641 agtccatcag gaagcggaac atttgggggt tgtcggtttc cagtttttta tcgacggcga
     2701 cgcagcggat gttgtccacc agcatggggc gcacccattt cgaataggaa agcctgtggt
     2761 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2821 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2881 cggcttcctg aaaagaaatg tctagcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2941 gatatgccgt ctgaaaagta caggattcgt gcagtaaaac tttgcggcgg caaatgtgcg
     3001 ataatacgcg gcgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3061 acgtataacc ggatacccgc ctgacgcggg ttttttacgg aagggggcaa aaatgcctaa
     3121 tccgctttac agacagcaca tcatctcgat ttcggatttg tcgcgcgaac agttggaatg
     3181 cctgcttcag acggcattga agctgaaggc gcatccgcgc ggcgatttgt tggaaggcaa
     3241 acttatcggt tcgtgcttct ttgaaccgtc cacgcgcacg aggctgtcgt ttgaaacggc
     3301 ggtgcagcgg ctgggcggca aggtcatcgg cttttccgac ggcgcgaata ccagtgccaa
     3361 aaaaggcgag acgcttgccg ataccgcccg catcatttcc ggctacaccg acgcaatcat
     3421 ccaacgccac cccaaagacg gcgcggcgcg cgtggcagcg gagttttcgc gcgtccccgt
     3481 catcaacgcc ggcgacggca ccaaccagca ccccagccag accttgttgg atctggtaac
     3541 gatttatgaa acgcaggggc gtttggacaa gctcaaaatc gccatggcgg gcgacttgaa
     3601 atacgggcgc accgtgcatt cgctttgtca ggcgttgaaa cgctggggct gcgaatttgc
     3661 ctttgtttcg ccgcccagcc tcgcgatgcc cgactacatt accgaagagt tggacgaagc
     3721 cggctgccga taccgtatcc tcggcagttt ggaagaggcg gcggaatggg cggacatcct
     3781 gtatatgacc cgcgtccagc gcgaacgttt cgatgaacag gaatttgcca aaatccaagg
     3841 caaattcaac ctcgaagcgt ctatgctcgc ccacgccaaa ccgaacctgc gcgtgctgca
     3901 ccctttgccg cgcgtggacg aaatccatcc cgatgtcgat gccacgccgc atgcctatta
     3961 tttcgagcag gcaaccaacg gcgtttatgc gcgtatggcg atattgtcgc tggtgttgaa
     4021 cgaagaagtg tgaggaaccg atatggaaac cccgaaactc agtgtcgaag ccattgaaaa
     4081 aggtacggtt atcgaccata ttcccgctgg cagggggctg accatcctgc gccagttcaa
     4141 acttttgcac tacggcaacg cagtaaccgt gggcttcaac ctgcccagca aaacccaagg
     4201 cagcaaagac atcatcaaaa tcaaaggcgt gtgcttggac gacaaagccg ccgaccgcct
     4261 tgccctgttt gcacccgaag cagtggtcaa caccatcgac aatttcaagg ttgtgcagaa
     4321 acggcatttg aacctgcccg acgaaatcgc cgaagtgttc cgctgtccga acacgaattg
     4381 cgccggccac ggcgagccgg tcaaaagccg gttttatgtt aaaaaacaca acgggcagac
     4441 gcggctgaaa tgccattact gcgaaaaaac ctacagccgg gattcggtgg cggaagcctg
     4501 acggattccc ttaaaccgag cgggcggcat ttcgtctgcc gcttgttttg ccaatctgaa
     4561 atggaatgat gatgcacgct tctgtccaaa gccgtttcgc accgatactt tatgttttga
     4621 ttttctttgc cggttttttg accgcgcaaa tctggttcaa tcagaaagcc tatactgaag
     4681 agctgcctcc gcttctgtcc gcattgtccg ctgtcgcgct ggtgtggctg gcgtgggcgt
     4741 tcgtgtcggc gcgttcaaag gctaaggcgg aaaagttcta ccgcgaaaaa atgatacaga
     4801 acgaaagcat acaccccgtc ctgcacgctt ctttgcaaca cttggaacac aagccgcaaa
     4861 tgctcgccct gctggtcaaa aaccacggca aagggatggc ggaacaggtc aggttcaagg
     4921 cggaagtgct gcccgacgac gaagacgcgc gcacgattgc cgccgagttg gcaaaaatgg
     4981 atatgtttgc attggggacg gacgcggtcg cctcgggcga aacctatgga cgcgtgttcg
     5041 ccgatatttt cgagttgtcg gcggctttgg aagggcgcgc gttcaaagga atgttgaaac
     5101 tgacggcgga atataaaaac atcttcggcg atgcctgccg ttcggaaacg gcgttggagt
     5161 tgggcgcgct caatcaggcg ttgcaggaga tttcaaaaac atcggaaaag tccaaacgga
     5221 tattttattg aagatggaaa aatgccgtct gaaacggaag gtgtttcaga cggcattttt
     5281 gtcggatgat taattattcg gagcggttga agccaaactt cacgcggctg cggccctgat
     5341 ccggtatatt gtccaaatcg cgtcccggat tggcggcggt gtcgcctacg gaaatatcgg
     5401 agatgttttc caaaatgatg gcggacgaca ggtgttcgga ggtgcggtaa accattgcca
     5461 agcccacttc ttcggcagga gtggaaatca gctcgacggt atccctgctt ttgaaattgt
     5521 tggagaggtc gacctgcatc gttttcttgc gtttgtagag gctcaaaacc gtgcctttgt
     5581 ccaaaccgtc cgcctcgcct ttgtcgatgg tgatggtttg aaactggccg gcaatccttg
     5641 tgccttcaaa cacggaaacg attttagcct gaaccgggcg ggacggttcg tgcggcatca
     5701 tgttgaagcg gtcggtgtct tccggcattt tcatcaggta gtcgccctgc tgtatttcgg
     5761 aaatggcggt ttcgaccacc agcggctgta tcgaaggggt gcgcagcggg gtaatcaaag
     5821 gatgggtgcg ggtatggtat tcgttgtctt tcagccgttc tccagcctgt ttcgagcgtt
     5881 gttcgagggc ggagtcggta tagtcgaggg agcgcacgat gccgctgaat gcgacttcct
     5941 gcccgaggaa tttacccgta tccggatcgg tgatgttttt attgattcgg taggtcaggt
     6001 agcggcccgg ctctttcagg cctttggtgt aaaccctggt gcctttggtg tacagcagcc
     6061 tgccttccgg gcccgagagc aggcgcggcg cggcagcggt ttctttgcgg gaaacgattt
     6121 gcggatgccg cataaagatg cggtagaagt tgacatcgat ggcgggaata ccgtatccgg
     6181 acacttcctt atccggactc attttgacga cggggatgcc gtctgtctgt tccaagccga
     6241 ggcgcggttc gccgtcaacg tggcgcaaca ccaatacctg gtccggataa atcaggtcgg
     6301 gattgtggat ttgatcccgg ttcgcgtccc acaggcggct ccattgccac gggctgtaca
     6361 ggtatttgcc cgaaatgccc cacagggtgt cgccctgttt gaccgtgtag cgttccggcg
     6421 cgttcgggcg cacctccaaa tttgccgcca aagtttgtgt tgagaatgcc atacctgccg
     6481 cgcagagcag ggttataata cgacgttgca taaccgttcc ccttatctga taaatttcgg
     6541 tttgtcttgc ttgattgggt tggaaaaagc ggcggcagcc cctcgggatg tgccgcgtga
     6601 taaaaaatgt tccgcatttt aacatcgaat tatccgcacc atcacggtaa ttatgaaaaa
     6661 caggcggcgt atccgccgaa ggaaagagaa aattatggct ttattgaata tcttgcaata
     6721 tcccgacgag cgtctgcaca cggtggcaaa gcctgtcgaa caagtcgacg agcgcatccg
     6781 gaagctgatt gccgatatgt ttgaaacgat gtacgaatcg cgcggcatcg ggctggcggc
     6841 gacgcaggtc gatgtgcacg agcgcgtggt cgtgatggat ttgaccgaag accgcagcga
     6901 accgcgcgtg ttcatcaacc ccgtcatcgt tgaaaaagac ggcgaaacca cttacgaaga
     6961 gggctgcctg tccgtgccgg gcatttacga caccgtaacc cgcgccgaac gcgtcaaggt
     7021 cgaggctttg aacgaaaaag gcgaaaagtt cacgctggag gcggacggct tgctggcgat
     7081 ttgcgtgcag cacgagttgg accacctgat gggcatcgtg tttgtcgaac gcctgtcgca
     7141 gctcaagcag ggacgcatca aaaccaagct gaaaaaacgt cagaaacata cgatttgacc
     7201 cttttgccgt gccgtctgaa cgctgcaaag ttttcagacg gcacggtctt gtccgacaac
     7261 cttacgcata cgcaggaata tgctatgaaa gtcatctttg ccggcacgcc cgattttgcc
     7321 gccgccgcct taaaagccgt tgccgccgcc ggttttgaaa tcccgctggt gctgacccag
     7381 cccgaccgtc cgaaagggcg cggtatgcaa ctgaccgccc cgcccgtcaa acaagccgcg
     7441 ctggaactcg gtttgcgcgt ggcacagccc gaaaaactgc gtaacaacgc cgaagccctg
     7501 caaatgctca aagaggtcga ggcagacgta atggtggttg ccgcctacgg tttgattctg
     7561 ccgcaggaag tgttggatac gccgaaacac ggctgcctca acatccacgc ttcgctgtta
     7621 ccccgttggc gtggcgcggc gccgattcaa cgcgcgattg aagccggcga tgccgagaca
     7681 ggcgtgtgta ttatgcagat ggacatcggt ttggacaccg gcgatgtggt cagcgaacac
     7741 cgctacgcca tccaaccgac cgataccgcc aacgaagtcc acgacgcgct gatggaaatc
     7801 ggtgcggcgg cggttgttgc cgatttgcaa cagcttcaaa gcaaaggccg tctgaacgcg
     7861 gtcaaacagc ccgaagaagg tgttacttac gcccaaaaac taagcaaaga agaggcgcgt
     7921 atcgattgga gcgaaagcgc ggcggttatc gaacgcaaaa tccgcgcctt caaccccgtg
     7981 cctgccgcgt gggttgagta tcagggcaag ccgatgaaaa tccggcgggc ggaagtggtg
     8041 gcgcaacaag gcgcggcagg cgaagtgttg tcctgttcgg cggacggttt ggtcgttgcc
     8101 tgcggcgaaa acgcgctgaa gattaccgaa ttgcagcctg ccggcggcag gcgtatgaat
     8161 atcgcggcgt ttgcggcggg acggcatatc gaagcaggga cgaagctgta aatcccttca
     8221 gacggcattc cgatccgcaa acgggaatgc cgtctgaaac catcagttga agaaagcgaa
     8281 tcacataata tgagtatggc acttgcccaa aaacttgccg ccgacagcat tgcggcggtt
     8341 gccgaaggac gcaaccttca ggacgtgttg gcgcaaatcc gcaccgcgca tcccgacctt
     8401 atggcgcagg aaaacggcgc gttgcaggac atcgcctacg gctgccagcg ttatttgggc
     8461 agtttgaaac atatgctcgc gcagatgctg aaaaagccga ttggcaatcc gcagctcgaa
     8521 agcctgcttt tggcggcgtt gtaccagctg cattacacgc gcaacgcgcc ccacgccgtg
     8581 gtcaatgagg cggtggaaag catcgcgaaa atcggacgcg ggcagtaccg ttcgtttgcc
     8641 aacgcggttt tgcgccgctt tttgcgcgaa cgcgacaagc ttgtggcttc ctgtaaaaaa
     8701 gacgatgtag cgaaacacaa cctgccgctg tggtgggtgg cttacttgaa aaaccattat
     8761 ccgaaacact ggcacaacat cgccgccgcg ctgcaatccc atccgccgat gactttgcgc
     8821 gtcaaccgcc gacacggcaa tgccgaaagc tatttggaaa aactggtggc ggaaggtatc
     8881 gccgcaaagg cgttggacga atatgcggtt acgttggaag aagccgtgcc ggtaaaccgt
     8941 ctgcccggtt tttcagacgg cattgtttcg gtacaggact tcggcgcgca gcaggcggcg
     9001 tatctcctta acccgaaaga cggcgaacgg attttggacg cgtgcgccgc gccgggcggc
     9061 aagacggggc atattttgga actggcggat tgccgcgtta ccgccttgga catcgatgca
     9121 ggccgtctga agcgtgtgga ggacaatatc gcgcgcctgg gctttcagac ggcatcggcg
     9181 gcgtgtgccg atgcgcggga tttggcggcg tggtatgatg gaaagccttt cgacgcaatc
     9241 cttgccgacg tgccatgcac cgcttccggc gtggcgcggc gcaatcccga cgtgaaatgg
     9301 ctgcgccgtc cgaccgacgc gctcaaaacc gcccgccagc aggaagccct gctgggcgca
     9361 ctttggcaga cgctgaaaca gggcggcaga atgctgcttg ccacctgttc cgtgttcgtc
     9421 gaagaaaacg acgggcaatt gcaaaaattc ctcaaccgcc atgccgatgc cgaaccgatc
     9481 gaatcgcggg tgcttttacc gaacaaacac caagatggct tttattacgc gcttattcaa
     9541 aagcattaaa caatggcttg tgctgctgcc gatgctttcc gttttgccgg acgcggcggc
     9601 ggaggggata gatgtgagcc gcgccgaagc gaggataacc gacggcgggc agctttccat
     9661 cagcagccgc ttccaaaccg agctgcccga ccagctccaa caggcgttgc gccggggcgt
     9721 gccgctcaac tttaccttaa gctggcagct ttccgccccg ataatcgctt cttatcggtt
     9781 taaattgggg caactgattg gcgatgacga caatattgac tacaaactga gtttccatcc
     9841 gctgaccaac cgctaccgcg ttaccgtcgg cgcgttttcg acagactacg acaccttgga
     9901 tgcggcattg cgcgcgaccg gcgcggttgc caactggaaa gtcctgaaca aaggcgcgct
     9961 gtccggtgcg gaagcagggg aaaccaaggc ggaaatccgc ctgacgctgt ccacttcaaa
    10021 actgcccaag ccatttcaaa tcaatgcatt gacttctcaa aactggcatt tggattcggg
    10081 ttggaaacct ctaaacatca tcgggaacaa ataatgcgcc gttttctacc gatcgcagcc
    10141 atatgcgccg tcgtcctgtt gtacggactg acggcggcaa ccggcagcac cagttcgctg
    10201 gcggattatt tctggtggat tgttgcgttc agcgcaatgc tgctgctggt gttgtccgcc
    10261 gttttggcac gttatgtcat attgctgttg aaagacaggc gcgacggcgt attcggttcg
    10321 cagattgcca aacgcctttc tgggatgttt acgctggttg ccgtactgcc cggcgtgttt
    10381 ctgttcggcg tttccgcaca gtttatcaac ggcacgatta attcgtggtt cggcaacgat
    10441 acccacgagg cgcttgaacg cagcctcaat ttgagcaagt ccgcattgaa tttggcggca
    10501 gacaacgccc tcggcaacgc cgtccccgtg cagatagacc tcatcggcgc ggcttccctg
    10561 cccggggata tgggcagggt gctggaacat tacgccggca gcggttttgc ccagcttgcc
    10621 ctgtacaatg ccgcaagcgg caaaatcgaa aaaagcatca acccgcacaa gctcgatcag
    10681 ccgtttccag gtaaggcgcg ttgggaaaaa atccaacagg cgggttcggt cagggatttg
    10741 gaaagcatag gcggcgtatt gtacgcgcag ggctggctgt cggcgggtac gcacaacggg
    10801 cgcgattacg ccttgttttt ccgtcagccg gttcccaaag gcgtggcaga ggatgccgtc
    10861 ttaatcgaaa aggcaagggc gaaatatgct gagttgagtt acagcaaaaa aggtttgcag
    10921 acctttttcc tggcaaccct gctgattgcc tcgctgctgt cgatttttct tgcactggtc
    10981 atggcactgt atttcgcccg ccgtttcgtc gaacccgtcc tatcgcttgc cgagggggcg
    11041 aaggcggtgg cgcaaggcga tttcagccag acgcgccccg tgttgcgcaa cgacgagttc
    11101 ggacgcttga ccaagttgtt caaccacatg accgagcagc tttccatcgc caaagaagca
    11161 gacgagcgca acaggaagcg cgaagaagcc gccaggcatt atcttgaatg cgtgctggag
    11221 gggctgacca cgggcgtggt ggtgtttgac gaacaaggct gtctgaaaac cttcaacaaa
    11281 gcggcggaac agattttggg gatgccgctt acccccctgt ggggcagcag ccggcacggt
    11341 tggcacggcg tttcggcgca gcagtccctg cttgccgaag tgtttgccgc catcggcgca
    11401 gcggcaggta cggacaaacc ggtccatgtg aaatatgccg cgccggacga tgccaaaatc
    11461 ctgctgggca aggcaaccgt cctgcccgaa gacaacggca acggcgtggt aatggtgatt
    11521 gacgacatca ccgttttgat acacgcgcaa aaagaagccg cgtggggcga agtggcaaaa
    11581 cggctggcac acgaaatccg caatccgctc acgcccatcc agctttccgc cgaacgtctg
    11641 gcgtggaaat tgggcgggaa gctggatgag caggatgcac aaatcctgac gcgttcgacc
    11701 gacaccatcg tcaaacaggt ggcggcattg aaggaaatgg tcgaagcatt ccgcaattat
    11761 gcgcgttccc cttcgctcaa attggaaaat caggatttga acgccttaat cggcgatgtg
    11821 ttggcattgt atgaagccgg tccgtgccgg tttgcggcgg agcttgccgg cgaaccgctg
    11881 atggtggcgg cggatacgac cgccatgcgg caggtgctgc acaatatttt caaaaatgcc
    11941 gccgaagcgg cggaagaagc cgatgtaccc gaagtcaggg taaaatcgga agtggggcgg
    12001 gacggtcgga ttgtcctgac ggtttgcgac aacggcaaag ggttcggcag ggaaatgctg
    12061 cacaacgcct tcgagccgta tgtaacggac aaaccggcgg gaacgggatt gggtctgccc
    12121 gtggtgaaaa aaatcattga agaacacggc ggccgcatca gcctgagcaa tcaggatgcg
    12181 ggtggggcgt gtgtcagaat catcttgcca aaaacggtaa aaacttatgc gtagcagcga
    12241 tattttaatt gtagacgacg aaatcggcat ccgcgacctg ctgtcggaaa tcctgcagga
    12301 cgaaggttat tcggtcgcat tggcggaaaa cgccgaagag gcgcgcaagc tgcgccatca
    12361 ggcgcgtccg gcgatggtgc tgctggatat ttggatgccc gattgcgacg gcatcaccct
    12421 tttgaaggag tgggcgaaaa acgggcagct taatatgccg gtcgtcatga tgagcgggca
    12481 tgccagcatt gacacagcag tggaagccac caaaatcggt gcgctcgact ttttggaaaa
    12541 accgatctcc ctgcaaaaac tgctgtctgc cgtcgaaaac gcgttgaagt acggtgcggc
    12601 gcaaaccgaa acggggcctg tattcgacaa gctgggcaac agtgcggcga ttcaggagat
    12661 gaaccgcgaa gtcgaagcgg cggcaaagcg cacgtcccct gtatttttga cgggtgaggt
    12721 gggttcgccg tttgaaaccg tagcgcgcta tttccataaa aacggtacgc cgtgggtcag
    12781 tccggcaagg gtcgaatatc tgatcgatat gccgacggaa ctgttgcaga aagctgaagg
    12841 cggtgtttta tatgtgggcg atattgccca atacagccgc aatatccaaa ccggcattgc
    12901 ctttatcatc ggcaaggcag aacgctgccg cgtcagggtg gtcgcatcgg gcagcagggc
    12961 ggcaggttca gacggcattg cctgcgagga aaagctggcg gaactgctgt cggaatcggt
    13021 cgtccgtatt ccgccgctgc gtatgcagca tgaagacatt cccttcctga tacaggggat
    13081 tgcctgcaat gtggcggaaa gccaaaagat tgcgcctgcc tcattcagtg aagaggcact
    13141 tgccgcattg acccgttacg actggccggg aaatttcgac caactgcaaa gcgtcgttgc
    13201 aacgctgttg ttggaggcgg acggacagga aatcggcgca ggggcggttt cttccctttt
    13261 ggggcagaat gtgcctgccg agggagcgga agatatggtg ggcgggttta atttcaacct
    13321 gcccctgcgc gaattgaggg aggaggtgga gcggcgttat ttcgagtacc acatcgccca
    13381 agaaggtcag aatatgagca aagtggcgca gaaagtcggt ttggaacgca cgcaccttta
    13441 ccgcaaactc aaacagctcg gcatcggcgt ttcgcgccgg gcgggggaaa aaaccgaaga
    13501 ataggcccgg acggccggtt ttccggctgc gggcttttgt tttcagacgg catttggtgc
    13561 aaatgccgtc tgaaatcgta aggggacgga ttttatgaca gaggacgaac gtttcgcgtg
    13621 gctgcaattg gcgtttacgc cctatatcgg cgcggaaagc ttcctgctgc tgctgcgccg
    13681 tttcggcagc gcgtcaaacg ccctgtccgc accggcggaa caggtcgccc cggcggtgcg
    13741 gcacaagcag gcggccgagg cttggcgcaa tgcggaaaaa cgcgcctcgg cgcggcaggc
    13801 ggcagaagcg gcgttggaat gggaaatgcg ggacggatgc cgtctgatgc tgcttcagga
    13861 tgaagatttc cccgaaatgc tgacgcaggg gctgaccgcg ccgccggttt tgtttttgcg
    13921 cggcaacgtg cggctgctgc acaagccttc cgccgccatc gtcggcagcc gccatgccac
    13981 gccgcaggcg atgcggattg ccaaagattt cggcaagtcg ttgggcgggc aaaatatccc
    14041 cgttgtgtcg ggtatggctt ccggtatcga tactgccgcc catcagggcg cactggaggc
    14101 agaaggcggc accatcgccg tgtgggggac gggtatagac cgcatttacc cgccgtccaa
    14161 taaaaacctt gcctatgaaa tcgccgaaaa aggattgatt gtcagcgagt ttcccatcgg
    14221 tacgcgcccg tatgccggca actttccgcg ccgcaaccgc ctgattgccg cgctgtcgca
    14281 ggttacgctg gtggttgaag ctgcgttgga atccggttcg ctgattaccg ccggcttggc
    14341 ggcagaaatg gggcgcgaag tgatggcggt acccggttcg atagacaatc cgcacagcaa
    14401 aggctgccac aaactgatta aagacggcgc gaaattggtg gaatgcctgg acgacatcct
    14461 gaacgaatgc ccggggctat tgcaaaatac gggtgcttca tcatattcta taaataagga
    14521 tacgcccgac acgggcagtc gcgccgttca gacggcatac gccccgccgc ctgccgcaaa
    14581 aatgccgtct gaaggggcgg caggcggcac agccgtcggc ggcatactgg ataaaatggg
    14641 tttcgacccc atccatcccg acgtgcttgc cggacagttg gctatgcctg ccgcagattt
    14701 gtatgccgca ctgttggaat tggaattgga cggcagcgtt gccgcaatgc ccggcggcag
    14761 ataccagcgt atccgaactt gaacgcactt tatattaagg aacacgaatg accgaagtca
    14821 tcgcctacct catcgaacat ttccaagatt tcgatacctg tccgccgccc gaagacttgg
    14881 gtatgctgct tgaagaagcg ggtttcgata cgatggaaat cggcaacacc ctgatgatga
    14941 tggaagtatt gctcaacagc tccgaatttt ccgccgaacc tgccgacagc ggcgcattgc
    15001 gcgtgtacag caaagaagaa accgacaacc tgccgcagga agtgatgggg ctgatgcagt
    15061 atctgattga agaaaaagcc gtcagctgcg aacagcggga aatcatcatc cacgcgctca
    15121 tgcacattcc gggcgacgaa attaccgtag ataccgccaa agtgctgacc ctgctgcttt
    15181 tatgggcaaa caagagcgag ctgcccgtgt tggtcggcga cgagctgatg agcgcgcttt
    15241 tactcgacaa caaacccacg atgaactgaa gcggcttcag acggcccgcc cgagtccgtc
    15301 tgaaacgtcg gcatcaaaac caccatccag agaacgacaa atggcgaaaa acctattaat
    15361 cgtcgaatcc ccgtccaaag ccaaaaccct gaaaaaatat ttgggcggcg attttgaaat
    15421 ccttgcatcc tacggacacg tccgcgacct cgtccccaaa agcggcgcgg tcgatcccga
    15481 caacggcttt gcgatgaaat accaactcat cagccgcaac ggcaaacacg tcgatgccat
    15541 cgtcgccgga gccaaagaag ccgaaaacat ctacctcgcc accgacccgg atagggaagg
    15601 cgaagcgatt tcttggcatc ttttggaaat cctcaaatcc aaacgcggcc tgaaaaacat
    15661 caagccgcag cgtgtcgtgt tccacgaaat caccaaaaac gccgtgctcg atgccgttgc
    15721 ccatccgcgc gaaatcgaaa tggacttggt cgatgcgcaa caagcccgtc gcgctttgga
    15781 ctatttggtc ggctttaacc tttcgccatt gttatggaaa aaaatccgcc gcggtttgag
    15841 cgcgggtcgt gtgcaaagtc ccgctttgcg cctgatttgt gagcgtgaaa acgaaatccg
    15901 cgcgtttgaa gcgcaggaat attggacggt gcatctcgac agccacaaag gccgcagcaa
    15961 gtttaccgcc aaactcgccc aatacaacgg cgcgaaactc gaacaattcg acctgccgaa
    16021 cgaagccgcg caagccgacg tgttgaaaga actcgaaggc aaagaggccg tcgttaccgc
    16081 cattgaaaag aaaaagcgca gccgcaatcc cgccgcgccg ttcaccactt ctaccatgca
    16141 gcaggatgcc gtgcgcaaac tcggcttcac caccgaccgc accatgcgta ccgcccaaca
    16201 gctttatgaa ggtatagacg tagggcaggg cgcgatcggt ctgattacct atatgcgtac
    16261 cgacagcgtg aatttggcgg atgaagcctt aaccgaaatc cgccattaca tcgaaaacaa
    16321 aatcggcaaa gaatatctgc cgagtgccgc caaacaatac aaaaccaaat ccaaaaacgc
    16381 ccaagaagcg cacgaagcca tccgcccgac ttccgtgtac cgcacgcccg aaagcgtcaa
    16441 acccttcctg agcgcagacc agttcaaact ctatcaaatg atttggcagc gtaccgtcgc
    16501 ctgtcagatg acgcccgcca aattcgacca aaccaccgtc gatattaccg tcggcaaagg
    16561 cgtattccgc gtaaccggac aagtgcagac tttcgcaggc ttcctcagcg tttacgaaga
    16621 aagcagcgac gatgaagaag gcgaagacag caaaaaactg cccgaaatga gcgaaggcga
    16681 caaattgccc gtggacaaac tctacggcga acaacacttt accactccgc cgccacgcta
    16741 caacgaagcc acgctggtta aagccctcga agaatacggc atcggccgcc cctcgaccta
    16801 cgccagcatc atctccacgc tcaaagaccg cgaatacgtt acccttgagc aaaaacgctt
    16861 catgcccacc gacacaggcg acatcgtcaa taaattcctg accgaacact tcgcccaata
    16921 cgtcgattac cacttcactg ccaaactcga agaccagctt gacgaaattg ccgacggcaa
    16981 acgccaatgg attcccttga tggacaaatt ctggaaaccg ttcatcaaac aagtggaaga
    17041 aaaagaaggc atcgaacgcg ccaaatttac cacgcaggaa cttgacgaaa cctgccccaa
    17101 atgcggcgaa cacaaactgc aaatcaagtt cggcaaaatg ggtcgcttcg ttgcctgcgc
    17161 cggttatcca gagtgcagct acacccgcaa cgtcaacgaa actgccgaag aagccgccga
    17221 gcgcatcgcc aaagccgaag cagagcaagc cgaacttgac gggcgcgaat gccccaaatg
    17281 cggaggacgg ctggtgtaca aatacagccg aaccggcagc aaattcatcg gctgcgccaa
    17341 ctatccgaaa tgcaaacacg tcgagccgtt ggaaaaaccg aaagacaccg gcgtccaatg
    17401 cccgcaatgc aaaaaaggca acctcgtcga gcgcaaatcc cgctacggca aactgtttta
    17461 cagttgcagc acctatcccg actgcaacta cgccacctgg aacccgcccg ttgcagaaac
    17521 ctgtccaaaa tgcgcgtggc cggttttgac catcaaaacc accaaacgct ggggtgtgga
    17581 aaaagtctgc ccgcaaaaag aatgcggctg gaaagaacag attgaaccgc ctgcaccgaa
    17641 agagtgaagc ggttggggtt gagatgaaag aaaatgccgt ctgaaagggg tttcagacgg
    17701 cattttcttc atgtgaaaac aaacctactc agattgaaac tgtttacaca cttctatatt
    17761 aatctgtata aagtcctgcc acgacatttg ttctgataag taatggtgat aatagtcctc
    17821 cacacgacag accaaagggc gcgtttcata gatagtgcat aagcgcgttg aatcatcgaa
    17881 atggcggcag gtaccatctc cccgatccag gtatatggtt tgttcgctta aattgacctt
    17941 ttgacagcac aaaccgcaag cagagcatgg gaagggttct gatgaggcaa tcaaaaatcg
    18001 attactttca cattgtcgtt tttcaaagct gcaatcagat tgtccacctg tttcagtgga
    18061 gaatctttgg caatgctcac aatgccgtta attgctgtat caatgacgtg gatatttccc
    18121 gcttccattc ctttgtccaa tgcctcaaat aaaccgtcaa tcattttgcg acgttctttg
    18181 aacgtatttt ccagatacaa gcgcaaaatt tcttgttggt ttttcaattc tgctaatcgg
    18241 acatcacgcc atgcaccaat gacttcacgt ttggttttct cttgtgcagc aattttctta
    18301 tattcgttgc gtgcttctaa gatgcgaaaa aacgaattag aagctgcatc aaacggaatg
    18361 gttgctggat tctttgttgg tagcttatta aggatttgga tttttcccat tatactgtac
    18421 ctaattcgat attcagacaa ccttcgcatt gatgacggat attggctaca ggagatccgt
    18481 cctcttccat aataggcgta ttcagcagat ttttaatcgc cttgccgatg accagcatgg
    18541 tatggaaatc aggatggtcg gcatggtcga cacgaacctt ttcgaaacgt ttaaccattt
    18601 cttctaaggt atggcgtatt tcccttgcgt tttccttcaa cccttctaat ccttctgtaa
    18661 ttaaatccat ctcgtcaata gcctcatcga tttcagcact atatgctcgt gcttccgtca
    18721 gtgcctcttc cgccttgctt gccagcataa atccaccgat tgctaaagca ggtcctgcca
    18781 cgatgccacc caaagccacc atgccgcctg ccataccgaa tccacctgct gccaatgagc
    18841 cacctccgag ccaagcaagg gttgcattgg ttgccgcagc acctgaaagg gtggagattg
    18901 ctgttcctgt ggaagcagtt gcaatcatag gtactgcacc gtatacgcca aagcctgcca
    18961 gtgcgccagt aatggcacta ctgccgagat tggtgcttaa ctctaaagaa gtttgcacca
    19021 tagtttccat ttcttttaat tcagcaatat caaatgtact gttaaaaccc gacagttcgc
    19081 ttttcgcatg cttgttgctg gtgttgttta ggacttgaac aatatgtctg atttgattgt
    19141 taaaaatatc aaccttaaac tgtcccagct cctcaaaagc aaggttggtt tcgtctttag
    19201 tcgaattcaa ttctcttttc ctgcgtttat aacggcgttc tgcacgctca cctatttctt
    19261 ttgcatcatc tatatcgctg agtgcagctg ctcctttgac aatacctgtt gcagcaaggg
    19321 cggcggctgc accccaaagt aaaactggaa tagccatgtg taatttccta aagtgtataa
    19381 aaagttaaaa atcttgaagt ttcaatgtgt attgtaattt taaaattcta ttaaaaatac
    19441 atttgccatt ccagcaaact aaagtttaaa atctatccaa ataatttaac catctataca
    19501 gaaagttcag ctcatggaaa cccacgaaaa aatccgcctg atgcgcgaat tgaacaaatg
    19561 gtcgcaggag gatatggcgg aaaagctggc gatgtcggca ggcgggtatg ccaaaatcga
    19621 acggggcgaa acgcagttga atatcccgcg tttggagcag ttggctcaga ttttcaaaat
    19681 cgatatgtgg gacttgctca aatcgggcgg cggcgggatg gtgtttcaga ttaatgaagg
    19741 tgatagtggt ggcgatattg cgttgtatgc gtcaggtgat gtttcgatga aaatagaatt
    19801 tttaaaaatg gagttgaaac attgtaaaga aatgttggaa caaaaagaca aagaaatcga
    19861 gttgctccgc aagctgaccg aaaccgttta aacagatatg ccgtctgaaa aaagttttca
    19921 gacggcatat tctttgacag gtcttgtata ataccgtttg aacttacagg tttttgatta
    19981 tggcggcagg caaacatacc aaacacagca accaggtacg cattatcggc gggcaatgtc
    20041 ggggcaggaa attgagtttt gcatccgcca acggactgcg tccaaccccc gatagcgtgc
    20101 gtgaaaagct gtttaactgg ctgggacagg atttgacggg taaaacggtt ttggatctct
    20161 tcggaggcag cggcgcactc ggtatagaag ccgcttcgcg caacgccaaa cgcgtgctga
    20221 tttcggataa caaccgccaa accgtgcaga ccttgcagaa aaacagtcgc gaactgggtt
    20281 tggggcaggt gcaaatcgtc tgttcagacg gcatcgcata tttgaagacc gtatccgaac
    20341 agtttgatgt cgtctttctc gacccgccgt ttgcatggca ggactggcaa atcctgttcg
    20401 atgccttgaa gccgtgcctg aacccccggg cattcgtcta tctcgaggcg ggtaggctgc
    20461 cggatatccc cgattggctg acggaatata gagaagggaa atcagggcag agtacattcg
    20521 aattaagggt tttccaagtg gctgaataat atgcgctttg ataatcattt ccgagttgta
    20581 aacattcgtt tgcaaccgtc cggttcaaaa aaaccttgtg ctataatccg cgcccgcccg
    20641 gttttgataa tttagtggaa aaggaaaaga aatgtcgctt tttattaccg acgagtgcat
    20701 caactgcgac gtatgcgaac ccgaatgccc caatgatgcc atttcccaag gcgaggaaat
    20761 ttacgaaatc aaccccaacc tctgcacgca gtgcgtcgga cactacgatg agccgcagtg
    20821 ccagcaggtt tgcccggtgg actgcatcct gattgacgaa gaacatcccg aaacccatga
    20881 cgagttgatg gcgaaatacg aaaagattat ccagtttaaa taaattcttt ttaaaacatc
    20941 aaattatgtc tgttttgaaa taaaatcaaa aaaaaacttg acggaaaagc aagccgctaa
    21001 taaactaacg ttctcttttg gagggattcc cgagcggtca aagggggcag actgtaaatc
    21061 tgttgcgaaa gcttcgaagg ttcgaatcct tctccctcca ccaaaattct tacttggggc
    21121 agtagcgagt aatgcgggtg tagctcaatg gtagagcaga agccttccaa gcttacggtg
    21181 agggttcgat tcccttcacc cgctccaaac aattaggccc atgtagctca ggggtagagc
    21241 actcccttgg taagggagag gccggcagtt caaatctgcc catgggcacc atctctcgat
    21301 tattcatttc tttaaggctt agatatatag gatattg
//
LOCUS       DAWGJV010000017        21315 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWGJV010000017 DAWGJV010000000
VERSION     DAWGJV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.017"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..808
                     /gene="pglE"
                     /locus_tag="WHQ94_000459"
     CDS             <1..808
                     /gene="pglE"
                     /locus_tag="WHQ94_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HEZ2127793.1"
                     /translation="RYRTPNIKYIYQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINC
                     QVLLDFLGKDDSDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSAWNKIIKRELIIREKLFFERGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="WHQ94_000460"
     CDS             849..1379
                     /locus_tag="WHQ94_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ2127794.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1396..1527)
                     /locus_tag="WHQ94_000461"
     CDS             complement(1396..1527)
                     /locus_tag="WHQ94_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ2127795.1"
                     /translation="MPSEPEKRLSDGIFACNSNAAGQNQGASDTVIGHPVRMNLGLT"
     gene            complement(1536..3131)
                     /locus_tag="WHQ94_000462"
     CDS             complement(1536..3131)
                     /locus_tag="WHQ94_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ2127796.1"
                     /translation="MFQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCEDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3238..3633)
                     /gene="hisI"
                     /locus_tag="WHQ94_000463"
     CDS             complement(3238..3633)
                     /gene="hisI"
                     /locus_tag="WHQ94_000463"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ2127797.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3664..4431)
                     /gene="hisF"
                     /locus_tag="WHQ94_000464"
     CDS             complement(3664..4431)
                     /gene="hisF"
                     /locus_tag="WHQ94_000464"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ2127798.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4444..5181)
                     /gene="hisA"
                     /locus_tag="WHQ94_000465"
     CDS             complement(4444..5181)
                     /gene="hisA"
                     /locus_tag="WHQ94_000465"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ2127799.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5214..5852)
                     /gene="hisH"
                     /locus_tag="WHQ94_000466"
     CDS             complement(5214..5852)
                     /gene="hisH"
                     /locus_tag="WHQ94_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ2127800.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGYRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5983..7485)
                     /gene="pta"
                     /locus_tag="WHQ94_000467"
     CDS             complement(5983..7485)
                     /gene="pta"
                     /locus_tag="WHQ94_000467"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ2127801.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7670..8728)
                     /gene="fbpC"
                     /locus_tag="WHQ94_000468"
     CDS             complement(7670..8728)
                     /gene="fbpC"
                     /locus_tag="WHQ94_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ2127802.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8749..10266)
                     /gene="fbpB"
                     /locus_tag="WHQ94_000469"
     CDS             complement(8749..10266)
                     /gene="fbpB"
                     /locus_tag="WHQ94_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ2127803.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10334..11329)
                     /gene="fbpA"
                     /locus_tag="WHQ94_000470"
     CDS             complement(10334..11329)
                     /gene="fbpA"
                     /locus_tag="WHQ94_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ2127804.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11580..12050)
                     /locus_tag="WHQ94_000471"
     CDS             complement(11580..12050)
                     /locus_tag="WHQ94_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ2127805.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12107..13483)
                     /gene="argH"
                     /locus_tag="WHQ94_000472"
     CDS             complement(12107..13483)
                     /gene="argH"
                     /locus_tag="WHQ94_000472"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ2127806.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRTAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13502..14371)
                     /gene="galU"
                     /locus_tag="WHQ94_000473"
     CDS             complement(13502..14371)
                     /gene="galU"
                     /locus_tag="WHQ94_000473"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ2127807.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14399..14998)
                     /gene="rdgB"
                     /locus_tag="WHQ94_000474"
     CDS             complement(14399..14998)
                     /gene="rdgB"
                     /locus_tag="WHQ94_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ2127808.1"
                     /translation="MSEKPEKIVLASGNVGKLEEFGNLFKPYGITVLPQSEFGIPECP
                     EPYSTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(14991..15224)
                     /locus_tag="WHQ94_000475"
     CDS             complement(14991..15224)
                     /locus_tag="WHQ94_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127809.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVAAFLFA
                     FGALFGQIGALALYLRHKSLHAAQSAAKENRYV"
     gene            complement(15336..15869)
                     /locus_tag="WHQ94_000476"
     CDS             complement(15336..15869)
                     /locus_tag="WHQ94_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ2127810.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15971..16429)
                     /gene="nudB"
                     /locus_tag="WHQ94_000477"
     CDS             complement(15971..16429)
                     /gene="nudB"
                     /locus_tag="WHQ94_000477"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ2127811.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16640..16716
                     /locus_tag="WHQ94_000478"
     tRNA            16640..16716
                     /locus_tag="WHQ94_000478"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16674..16676,aa:Pro,seq:ggg)
     gene            16874..19273
                     /gene="mafB"
                     /locus_tag="WHQ94_000479"
     CDS             16874..19273
                     /gene="mafB"
                     /locus_tag="WHQ94_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ2127812.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYSAGNLSK
                     KVLTLVYINGKLKFGPSSLPSSALKAPVPIVGKAEAPPGKAVPVGEARIPSNPKAVKG
                     TTVTLSQEQRLANGELIPKGSVVTWSDNAVKVVRPDGKKRRFNSRDWEQKALPKPQLP
                     KDYRDDINIGGNNPRFNQQRHEKIESHNPNRIEEIRQRNAVSSAERQADALKRMQTSG
                     RTRLPGQPQKGKPGAAREMSSSDNPNQTAEAFARRYLGRKPTLMERVEGEKLYGGKCV
                     GCWKAQMPDGTFVVYRPAGNASMTKGTTASVDINNPTLNNLNINLKGKPTELKFKFPA
                     R"
     gene            19284..19745
                     /locus_tag="WHQ94_000480"
     CDS             19284..19745
                     /locus_tag="WHQ94_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127813.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            19771..20019
                     /locus_tag="WHQ94_000481"
     CDS             19771..20019
                     /locus_tag="WHQ94_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ2127814.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            20046..20465
                     /locus_tag="WHQ94_000482"
     CDS             20046..20465
                     /locus_tag="WHQ94_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127815.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            20543..20626
                     /locus_tag="WHQ94_000483"
                     /pseudo
     CDS             20543..20626
                     /locus_tag="WHQ94_000483"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20623..20865
                     /locus_tag="WHQ94_000484"
     CDS             20623..20865
                     /locus_tag="WHQ94_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ2127816.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCRYIDKKYIIPTGKKFSRDDFY"
     gene            20943..21188
                     /locus_tag="WHQ94_000485"
     CDS             20943..21188
                     /locus_tag="WHQ94_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ2127817.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTIKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
BASE COUNT         4936 a         4929 c         6141 g         5309 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggat tatcggaggc
       61 gagaaatacc ggaataaaaa attcaaacgg aaaatatata gcatttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttggtaaa gatgatagtg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcatatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtggaa taagattata aaaagagaat tgattattag
      361 agaaaaactg ttttttgaaa ggggaattta ttctgaagat atcgaatggt caatgaggtt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctattacgg gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttaa tagggatata tcgagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ctaataagtt
      721 gatatataag ctgatattcc cgataatcac attattcggg gttgatatta caggcaggat
      781 tttaaaagca atcaggggga atatttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttctg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaagggt
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gtatcggcaa ccgcgtcctg gtcggcatgg ggacgacggt tctggacgat gccgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgtgcctcc gcgcaaacgc ttggagggcg
     1261 gctatcttta tgtcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcctacg tcagccccaa attcatgcgg acgggatgcc cgataacggt
     1441 atccgatgcg ccttgatttt gaccggctgc gtttgagttg caggcaaaaa tgccgtctga
     1501 aagccttttt tcgggttcag acggcatttt atcgcctaaa gtttgaccga atgttcgcgc
     1561 gtttcgtgga acacgatgtc tggccaacgt tcttgcgtca accccaaatt cacgcggttg
     1621 ggggcgaggt aggcgaggtt gccgcccgcg tcgatggcga ggttgcccgc gttggctttt
     1681 tcaaattcag ccagcttttt cttgtcttcg cacgataccc agcgcgccga ccagatggat
     1741 gcgctgtcga acacggcttc tacgccgtat tcgttggcga ggcgcgaggt tacgacttca
     1801 aactgcaaca cgccgaccgc acccaaaatc aaatccgcgc cgctcatcgg tttgaacacc
     1861 tgtaccgcgc cttcttcgcc aagctgttgc aaaccttttt gcagttgctt gattttcagc
     1921 gggtttttga tgcggacgct gcggaacagt tcgggcgcga agaatgggat gccggtgaac
     1981 gccagttgtt cgccttcgga gaagctgtcg ccgatttgga tgttgccgtg gttcgggatg
     2041 ccgatgatgt cgcctgcgta ggcttcttcc accagctcgc ggtcgtgcga catgaatgta
     2101 accacgctgg aggcggcgat ttcgcggttg atgcgcaggt gtttcatctt catgccgcgc
     2161 tcgaatttac cggagcagac gcgcaagaag gcgatgcggt cgcggtgttt cggatccata
     2221 ttggcttgga ttttgaagat aaatccggag aatttcggct cgtccggctc gaccatgcgc
     2281 acggtcgcgt cgcgcggttt cggtgcaggc gcccattcaa tcaatgaatt gaggatttcc
     2341 tgaataccga agttgttaat cgccgaaccg aagaacacgg gcgtgagttc gccggcgaga
     2401 aattcgtcga gattgaactc gttggaagcc gcctgtacca attcgatttc gtcgcgcaac
     2461 tgctgtattt ctaacggaaa gcgttgttcc aattcgggat tatcgatgcc tttgatgatg
     2521 tcgaactcgt gcggcaagcg ttcgccgccc gcttcaaaga gatagatttc gtcgttcagg
     2581 atgtggtaca cgcctttgaa gtttttgccc atgccgattg gccaggtgac gggcgcgcag
     2641 cggatttgca ggatgttttc cacttcgtcc agcaattcca gggaatcgcg cacttcgcgg
     2701 tcgtatttgt tcatgaacgt aacaatcggc gtattgcgca ggcggcagac gttcaagagt
     2761 ttgatggttt gcgcttccac gccttttgcc gcgtcgatga ccatcaaggc gctgtccacg
     2821 gcggttaaaa cgcggtaggt gtcttcggag aagtcttggt gtcccggcgt gtccaaaagg
     2881 ttgacggtgt ggtctttgta gtcgaactgc atcacgcttg acgccacgga aatgccgcgc
     2941 tgcttctcga tgtccatcca gtcggaagtg gcgaatttgc cggttttctt gccttttacc
     3001 gtacccgcgc tttgaatcgc acctgaaaac agcaagagtt tttcagtcag cgtggtttta
     3061 cctgcgtcag ggtgggagat gatggcaaac gtgcggcggc ggcgcacttg gtcgaggatt
     3121 tcttggaaca tggttttctt tgcaaaaagg ttcaggccgc ttttcagacg gcccggacag
     3181 tgtttgagac ggcgaaattg tacaaaaaaa tgcctgataa ttcaatgttg gaggcggtca
     3241 gtgcgtgctg ccgtaaatct ctttttcgtc tttcaggacg gcatcggcgg tttcccacgc
     3301 gctgccacgc cagactttgt aaaagcagct ttctcgcccg gtgtggcagg cgatgccgcc
     3361 gttttgggca atgagcatca caatggcgtc gccgtcgcag tcgaggcgca gttcgcggac
     3421 tttttgcgtg tgtcccgact cttcgccctt catccattgt ttttggcgcg aacggctgta
     3481 atagtgggca aagccggttt cgacggtttt ttgcagggct tcggcgttca tccacgccac
     3541 cattaaaaca cgtttggttt cggcatcttg ggcgatggcg caaaccaaac ctttttcgtc
     3601 aaatttgacg gcttcaagca ggtttttatc catatttcct ttcagacggc atagtcggag
     3661 cggtcagagg cgcacttcga tgccggcttc gcgcatagcg cgtttggctt cgcggatggc
     3721 gatttccccg aaatggaaaa tgccggcggc aagtacggca tcggctttgc cttcggttat
     3781 accttcaatc aggtgccgga cattgccgac ccctccggag gcgatgacgg ggatgtcgac
     3841 ggcttcggca acggcgcggg tcagcggcag gttgaaaccc tgtttcgtac cgtccctgtc
     3901 catcccggtg agcaggattt cgcccgcgcc gcgtttttgc atttcgatcg cccattccac
     3961 cgcatccaaa ccggtcggat tgcgcccgcc gtgggtaaag atttcccagc gtgtgttttc
     4021 ggagttgacg gctttggcat cgacggcggc gacgatggct tgcgaaccga aaaatccggc
     4081 ggcttcgtca atcaggtcgg gacgggtaac ggcggcggtg ttgatgctga ctttgtccgc
     4141 gccggcattg agcaggcggc ggatgtcggc aacggtgcgt acgccgccgc cgacggtcag
     4201 ggggatgaag acttgtccgg caacctcttc gatgatgtgc aggatggtgt cgcggttgtc
     4261 ggatgaggcg gtgatgtcga ggaaggtcaa ttcgtccgcg ccttcgccgt tgtagcgttt
     4321 ggcggcttcg acggggtcgc ccgcgtcgcg caaaccgatg aagttcacgc ctttgacgac
     4381 gcgcccgtct tttacgtcga gacaggggat gatgcgtttt gccagtgcca taatcggatg
     4441 cctttagtcg agggaatctg ccagttgctg cgcttgggca aaatcgatgc tgccctcgta
     4501 aatcgcgcgg ccggtaatcg cgcctgctac gccatgtttt tcggcggcac acagggcgcg
     4561 gatgtcgtcc aagccggtca gtccgccgga ggcgatgacg ggaatgcgga cggcttgggc
     4621 gagtttgacc gtcgcgtcga tgttcacgcc gctcatcata ccgtcgcgcc cgatgtcggt
     4681 gtagatgatg ctgttgacgc cgtcgtcttc aaagcgtttt gccaaatcaa ttacatgatg
     4741 cccggttacg gttgcccagc cgtcgatggc ggccataccg tctttggcat ccagcccgac
     4801 aataatcctg ccggggaagg ctttgcacgc ctcgcgcacg aagtcggggt ttttgaccgc
     4861 cgccgtgccg ataatcacgt cgtttaagcc caaatccaaa tattgtccga tggttttcaa
     4921 atcgcgtatg ccgccgccga gctgtacggg gatgtctttg gcgacagcgg caaggatgtc
     4981 tttgatggcg ggcaggtttt gcggaacgcc ggcaaacgcg ccgttcaaat ctaccagatg
     5041 caggcggcgc gcgccttgtt tgaaccagtg cagcgcggtt tcggcgggcg aatcggaaaa
     5101 gacggtcgcc tcttccatca gcccttgttt caggcggacg cagcgtcctt ctttcaaatc
     5161 gatggcgggt atcagcagca taatttttct ccttgtgcgg ggccgtgtcc ggcttaccag
     5221 tttaaaaagt ttttcaacat cgtcagcccg gcatcgtggc ttttttcggt gtgaaattgc
     5281 gtggcgaata cgttgtcttt gccgacgatg caggcaaacg gggacgggta gtcgctttcg
     5341 cccaatatgg tttcgggatt ttcgggggcg aaatagtagc tgtggacgaa gtaaaaacgc
     5401 gtgtcttggg gaatatcttt aaacagcggg tggttttggg tttggcgcac ggtgttccag
     5461 cccatatgcg ggactttcag acggtatccc tgcgggtcgc ggaggtcgcg ctcaaagcgt
     5521 ctgactttgc cgccgaacca gcccaagccg tcggtgtttc cttcttcact gtggtcgaat
     5581 aaaagttgcg cgccgacgca gattccgaaa aagggtttgt tttttaaggc atctttgact
     5641 gcctcgtcca ggccgccgcg cgtcagtgcc gccatacagt cgggcatcgc gccctgcccg
     5701 ggaaaaatga ctttgtcggc gcgggacacg cgttcggggt cgccgcttaa aaagatttcg
     5761 gtattttttc cggcaagctg ccccgccgtc cggacggatt tcaatacgga atgcaggttg
     5821 cccataccgt aatcgataat ggcgatttgc atggcttcct cctctttttt tgcaatatgg
     5881 ctgcgatttt aacaaacaaa tgtgccgtgc tgataaaaat gccgtctgaa aacgggagtc
     5941 tgtcttcaga cggcataggg ttcaaacccg gaaagccgtt tgtcagcctt ccatttgttt
     6001 tgcctgaacg gcagtcaggg cgatggtgaa cacgatgtct tccaccagcg cgccgcgcga
     6061 caggtcgttg accggtttgc gcaggccttg cagcagcggg ccgacgctta agacgttggc
     6121 actgcgttgg acggctttat aggtgcagtt gccggtgttc aggtcgggga agaccaaaac
     6181 ggttgcctgt cctgccaccg ggctgcccgg ggctttggat ttgcccacgc ccggcacggt
     6241 tgccgcatca tattgcagcg ggccgtcgat ggcgaggtcg gggcgttttt cccgggcaag
     6301 tttggttgct tcgatgacgg tatcgacatc ggggccgctg ccggagttga cggtggagta
     6361 ggaaatcatc gccactttcg ggtcgatgcc gaaggctttt gcggaatcgg cagactggat
     6421 ggcgatgtcg gcaagctgtt gcgcggtcgg gttcggatta accgcgcagt cgccgaagac
     6481 gaggacttgg ttgggcagca gcataaagaa tacgctggac acgaggcttg cgcccggtgc
     6541 ggttttaatc agttgcaaag cggggcggat ggtgttggcg gtggtgtgaa ccgcaccgga
     6601 taccaaaccg tccacatcat tttgcgccat catcatcgta ccgagtacca cggtgtcttg
     6661 cagttgcttg cgcgcgtctt cgggtgtcag gcctttggat ttgcgcagtt cgcacatcgg
     6721 ctcgacgtat tgttcgacca atgaggcggg atcgatgatt tccaaagagt cgggcaggct
     6781 gatgccgcgt tctttggcaa cggcttcgac ttcttcgcgt ttggcaagca ggacgcagcg
     6841 ggcaatgcct ttttcgtggc agatggcggc ggcttggacg gtgcggggtt ctgcgccctc
     6901 aggcaggacg atgcgtttgt cggcttggcg ggcgaagtcg atcaggttgt agcggaattg
     6961 cgccggcgac aggcgttttg cttcgcggcc tgccaatacg gatacgtctt tcagcgcgtc
     7021 gctcgaaccg aagaaggtca ggccggtttt ttcggctgcc gcttcggcaa cggaggctgc
     7081 cgcgccgtcc acgacaaaac cttccaatac gcccggcgcg gcggcgaaga actgtttggc
     7141 aaggttcagc cgatttgcca gttcgtcggc atcggcgttg tcggaacgga cggcgaagac
     7201 ggctgccgcg tcaagggaca atgccagttc gacgtttttg cctgcgaggt agattttgtc
     7261 ggcatcgggc gcgatgcctt cgatgacgag gttggcggca tcgagtgcgg caactttgcc
     7321 gaccagtgcg tcgaaccagt cgtcgctttt gccttgcgcg agcagggttt cggcggttgc
     7381 gtcaacggct tggaaaattt gtgcgtccag tgcttttgca aaggcttgtg cggcggcgga
     7441 ggcgtccagt ccggcagata cgggtacgat gagtactttt gccatgatat atcctttcgt
     7501 atgctgcggt gtgcggcata tgtggttgga aggggcggca tataggcaga aacggctgcc
     7561 tgcgtgccgt gcgtgccgtg tttggcttga ggcgcgcagg ttgaatatag caaacaaatt
     7621 ctgtttccaa caagataaat atccgcaggc ttgtggatgc tgccgccttt cagagggtat
     7681 ttccggggaa gaacagggcg ggaccgtcca aatggaggac ggcggaaatg ccgtctgaca
     7741 gggtgggggc ggaagggagg ttgagcgtga ggacggtttg tccgacccgg agactgattt
     7801 cggtatgccg cgctttgggc gtggttttga gaaccacggc gtgaatggag gcggtgggtg
     7861 cggaatgggg gtgaaggctg aactgttccg gacggatgag cagtgtgccg cgtgtgcctg
     7921 cgggtgcgcc gctttgaacg ggcaggcggc ccaatccgca atcggcggtg ccgtcggcgt
     7981 tgagcgcggc ggggaacacg atgccttcgc cgataaacag ggcggcatca aggtcggcag
     8041 gttgtcggta caattcgtga gggcttgcag tttggaggat gcgcccctgt ttcatcacgg
     8101 caatccggtc ggcgtattgc agggcttctt cgcggtcgtg gctgacgaaa acggcagatt
     8161 tgccgttggc gcgcagggcg gcaatcatgt cttcgcgaat ctggcggcgc aactgttcgt
     8221 ccagcgcgct gaagggttcg tccaacaaaa tcagttcggg atcgggtgca agggcgcggg
     8281 cgagggcgac gcgctgttgc tgtccgcccg aaagttcgtg cggatagcgt ccggcaagtt
     8341 cggaaatgcc ggtcaattcc aacatagctt cgatgcgctg ccgttcttgc gccgtcttgc
     8401 ctttcccgtt gcccagcccg taggcggtgt tgcggtaaac ggtcaggtgg gggaacagca
     8461 caccttcctg tacgacataa cccaaacggc gttcgcggac ggggaggttg gtatttttcg
     8521 agaagatggt tctgccggaa agcgaaattt cgccaaaatc gggttgttca aaaccggcaa
     8581 ggcagcgtaa aagggtggtt ttgccgcagc cggacgcgcc gacgataaag aggatttcgc
     8641 ccgggtcgag gctgagcgaa atgtcgttta aaactggggt gttttgaaaa cttttggaca
     8701 ggtgtccgat gtgcagggcg gcggtcatgg cggtacttcc tcaagctgtt atttgaaggc
     8761 gtatttcttc agcaggaata cggggatgcc ggaaaataat accagcatca gcgcgtaagg
     8821 ggtggcggcg gcgtattgtg cgtccgatgt gtattcccaa acggcggtgg agagtgtgtg
     8881 gacatcgtcg gtggtcagca gcagggtggc ggtcagctct ttcatcagtt tgaggaagac
     8941 gagtgcgaat gcggcggtaa tgccgggcag gatggacggc agtaccaacg tcctgaaaat
     9001 aaagaagtgt ccgcgcccca atgttgcgcc gacctgttcc atccctttgg ggagttgttc
     9061 caaggaagtc ctcagggtgg tttgcgccat cggcaggtaa agcatgaaat aggcaaggat
     9121 gacgacgata aaggtttggt aaacggcagg ggtgtagttg atgctgaaat aaaccaagga
     9181 tagggcgata accaaaccgg ggacggcgtg cagtaaaaac ggcagcctgt ctatccaaac
     9241 ggttaaaaaa ttgcgatagc gaaccgatgc ccaaacaagg ggcaaggcac ataatatagt
     9301 caaaatcgca cctaaagccg atacgcttaa ggaacggata aaggcatcaa atacggatac
     9361 gagcgcgaat gtgccggaag tgccgaccat catccaatgt atcaatacgc caaaggggat
     9421 aataatgccc aaagtcaaca agctgcttaa aaaaacaatc gcgccgatct gaccgggcag
     9481 tttgagggtt ttgacgggat aaggacgggc aacgcctttg ccgctgtggt aaatcttggc
     9541 tttgccgcga aatatgcttt ctccaaatac gacgatgccg cacaccgcca ttaaaacagc
     9601 ggaaagcagg gcggcggtat tgttgttgta ggacatttcg tattcttgga aaatggcggt
     9661 ggtaaaagtg gggtagttca aaatagatac cgcgccaaat tcgaccagca tatgcagggc
     9721 aatcagtaac acgctgctgc cgatggcggg tttgagctgg gggaggatgg cggaaaaaaa
     9781 ggtttgcagg cggcttttgc ccaaggacag gctgacttct tcgtaagaca ggctgatgcg
     9841 tttgagtgcc gcctcgacgg gcaggtaggc gagcgggaac gaggacaggc tcataatcat
     9901 cactgtcccc caaaagcctt cgacacggaa ggtcaggctg atccaggtga aacagctgac
     9961 aaatgcgggg atgcacaaag gcagggtgat tgccgtctga aaaaaggttt tgccgaagaa
    10021 gcggtaacgt tggaacaaaa gggcgcaggc aatgcccaaa acaatggaaa tcagggtaac
    10081 gcccgccatc atcgtcaagg tgttggagag caaatcccac atacgcgggc ggaacaacag
    10141 ttcgacggcg cggttgatgc cgacctgcca cgaacgcata gcgacatata aaaaaggcag
    10201 ggtaagcggt agggcaatca gtaggatgag gccggtaagc caaatgggta tttttttagg
    10261 agacatagtg ttttttatcg gcaaaacggg cggacagtat aaatgtccac ccgtttgaca
    10321 atccgaaaac ggcttatttc ataccggctt gctcaagcag ccgggtggcg tgttcttttt
    10381 cggaaacagt ggtggcggac acttggggtg cttccaactt ggcgatgggt tccaaattga
    10441 aggtggatac cacgtgcgga ttcaaaggat actcggcacg gacggcggtc agggcgcgct
    10501 gtccttcctt gccggcgagg aaggcgacga attttttcgc ctcatccttg ttttgggagg
    10561 attttaacac ggctgcaccg gaataggtaa tgagtgcgcc gggatctctg tggcggacga
    10621 aattcaggcg ggtgtggacg ttttgtacgc ctttttcacg cgcgaaagcg tgccagtagt
    10681 agttgttgat gagggcggca tcgatttcgc cgttttcaac cgcttgaagg gcgacggagt
    10741 ttttagcgta aggcttgccg tattctttca gacctttgag ccatttcaat gcggccgctt
    10801 cgcctttcag tttgacgatg gcgacaacct gttccaagaa cgcgccggaa gtgggggcgt
    10861 aaccgatgcg gtttttccat ttcggcgtgg cgtaattcag gacggatttt tctaaatctt
    10921 tttcagacag tttgcgggtg tcgtaaacga cgacgcgcga acgtccgctc agtgccaccc
    10981 agtctttttt ggcggcaacc ggcacgccct tgccgcgtgt ttcgttgatg gtggaggcgg
    11041 gcaggggctc taggaggttg gctgcggaaa gggtggcgag tgccgggatt tgttcggaat
    11101 agaatacgtc ggcggggctt cggctgcctt cttctttgat ttgaccggca agctggtcgc
    11161 ctttggcgct gttgagtttg actttgatgc cggtagcccg ggtaaaggca tctgcaacgg
    11221 cttgtgctgc ttctttgtgt tggccgttgt acacggtaat gtctgccagc gcgggggttg
    11281 cggcggtcag ggctgcggca agcagtgcgt atcggataga tgttttcata tcgattttct
    11341 cctaatgaat gagagtgtac accttgttaa aacataacgg tgtgtagtgt attccttctt
    11401 ctttataaat gcaaataatt attttttaaa tttgttatta tccgatccgg ttattgtttg
    11461 ttctgacttg tattttttcc gtgcatcgcg cccgtaaggc ggaagcggcg ggcaatgcgt
    11521 ggcggaatgt gggtaaaggc ggcattttga tttgtcggaa tgcttgagaa cccctctctt
    11581 taaaacagcg tctgaacagg gttgccggaa tagtattgcc atcccggcag atacagtttg
    11641 tcggggtctt gccaatattg ttcatccaga ctgttcggca gtgaggcggt tttgttgtcg
    11701 agatgttttg ttatatccca catttctttc aggtttttac cttccgattg gaggtggcgg
    11761 atttcttttc tcaatgcgtc tgccgcccga tggatcagca ttgcgctgtg tatcgcgccg
    11821 atttttttca gaacggaaca agtcttttcg gcggcgggaa aaccccaatt gcagacaaat
    11881 tgcagtatgc cgccgttacc gatatcggct tctgtccgcc aaatcaaaac cagctcctgt
    11941 tcccgcccgt ccatgctttc gagtttgccg tcatgctgtt caaaaagttt gtccaccgcc
    12001 tgccacatca tctgttcgaa gcggtcggct tgggtatccg tatcggtcat cgtgttcttg
    12061 ccttttaaaa atgccgtctg aacatttctt cagacggcat cgggggttaa gccaacattt
    12121 cccgccagcg tttgacttgg aaacggactt gttcaggcgc ggtaccgccc aagtggttgc
    12181 gcgcgtttaa gctgccttcg ggtgtcagca cgccgtaaac gtcgtcggca atcaaatcgc
    12241 tgaaaccttg taagacttcg agcggcagtt tgctcaaatc gacacccgct tggtcggcgt
    12301 ggcgcacggc ttgggcgacg acttcgtggg catcgcggaa aggcatgcct ttttttacca
    12361 agtaatctgc caaatcggtg gcggtagcga agccctgcat cacggcggtg cgcatattgt
    12421 cgggtttgac ggttacgccg cgcatcatat cggcgtaaat ccgcaacgtg tcgataagcg
    12481 tgtcggcggt gtcgaacaag ggttctttgt cttcctgatt gtctttgttg tacgccaagg
    12541 gctgcgattt catcagggta atcaagccga taaggtgtcc gatgactcgg ccggatttgc
    12601 cgcgcacgag ttcgggcacg tcggggtttt tcttctgcgg catgatggac gaacctgtgc
    12661 agaaacggtc ggcgatgtcg ataaagccga aacgcggact catccacaaa atcaattctt
    12721 cagacaggcg gctcaggtga accataacca gcgaggcggc ggcggtgaac tcgatggcaa
    12781 aatcgcggtc ggatacggca tcgagcgagt tttggcagat ttgttcaaag cccaacagtt
    12841 tggcggtaat ttcgcgccga atcggataag tcgtaccggc aagggcggct gcgccgagcg
    12901 gcatacggtt gacgcggcgg cggcagtccg ccatccgttc gttatcgcgt ccgagcattt
    12961 cgacgtaggc gagcatatgg tgtccgaagc tgacgggctg ggcgacttgc aggtgggtaa
    13021 agcctggcat gacggtttcg gcgttttgtt ccgccaaatc cagcaatgcc gtctgaaggc
    13081 tttgaatcag gcttcggatg acggtaatct ggtcgcgcag ccaaaggcgg atgtcggtgg
    13141 cgacttggtc gttgcggctg cggccggtgt gcaggcgttt gcccgcgtcg ccgattttgt
    13201 cggtcaggcg gcgttcgatg ttcatgtgga catcttccaa atcggacgac cattcgattt
    13261 tgccgctgcg gatttcttcg aggatttccg ccatgccccg acggatgtcc gccaaatcgc
    13321 cttcgtccaa cacgccggtt tctttcaaca tttgcgcgtg tgccagcgag ccttgaatgt
    13381 cccattcggc aagccgtcgg tcgaaaccga tggaggcggt gtattgtttg acgagttcgg
    13441 aaacgggttc gttgaaacgt ccggaccagg ttttgtcgtg cataaggatt ccttgatggg
    13501 gttattcggt gcggtatttt tccaaaaggc ggcggaaggg ttcgccggtt tcggggtgtt
    13561 tcaggccgta ggcgacggtg gcttcgaggt agcccagttt gctgccgcag tcgtagcgcg
    13621 tgccttcaaa ggggtgcgcc aggacaaatt cgtgatcgag cagcttggcg atgccgtctg
    13681 taagctggat ttcgttgccc gcgccgcgcg gcagtccggt cagtaagtcg aaaatgcgcg
    13741 gggtaaggat gtagcgtccg acgacggcaa ggttggaggg cgcgtcttcg ggcttgggtt
    13801 tttcgacaat gccgataatg cgttggaact gtttgagctg ttcggtttcg acgatgccgt
    13861 atgagccggt ttgcgatgct tcaacggttt ctacgcccaa aatgctgttg ccgctgcgcc
    13921 cgtacacttc gaccatttgt ttgagcgcgc ctttgggggc atcaatcagg tcgtcggcaa
    13981 ggataacggc aaagggttcg tctccgatgg cggcgcgggc gcacaagacg gcgtgtccca
    14041 agcccagtgc ttccgcctga cggatgtaga ggcaggtaat gttcggcggc aggatgttgc
    14101 ggacgtgttc caacaatttg tctttatggc gcatttccaa ctcggtttcg agttcgtatg
    14161 ccttgtcgaa atggtcttcg atgctgcgtt tgttgcgtcc ggtaacaaac accatttccg
    14221 tgcagccggc ttccacggct tcttctacgg cgtattggat cagcggcttg tcgacgatgg
    14281 gcagcatttc tttcgggctt gccttggtgg cgggcaggaa gcgtgttccc atccctgcga
    14341 cggggaaaac ggctttccgt atgggtttca ttctttttcc tttgtattgt tttgatgttt
    14401 aaagggcgag tttgcgtaag agttcggcaa gtgcctgcgc gcggtggctt tcgcggtttt
    14461 tgacctccga atccaattcg gcggcggttt tgccgtgtcc gggcagataa aaatacgggt
    14521 cgtaaccgaa accgttttca ccggcgggaa cgccgttcca ttgtccgtgc catacgcctt
    14581 cggcaataat cggacgcggg tcgtctttat ggcggacaaa aaccaatacg cagacgtagc
    14641 aacagctttt gtcggctttg ccgacaagtt cggcggcaag tttcaggttg ttggcggtat
    14701 cggatttggg atggtcgccc gcgtaacgtg cggaatgtat gcccggtgcg ccgtttaggg
    14761 cggcggcaca gatgccgctg tcgtcggcga gtgcgggcag cccgctgtat tcggcagcat
    14821 ggcgtgcttt tgccagcgcg ttttcgacaa aggtggaata gggttccgga cattcgggta
    14881 tgccgaactc ggattgcggc aatacggtga tgccgtaagg tttgaatagg ttgccgaact
    14941 cttcgagctt gccgacatta ccgcttgcca aaacgatttt ttccggtttt tcagacatag
    15001 cggttttcct ttgcggcgga ttgggcggcg tgtagggatt tgtgccgcaa atagagggcg
    15061 agtgcgccga tttgtccgaa cagcgcgccg aatgcgaaaa ggaaggcggc gacggcaaag
    15121 gctttgctgc cggcggtcag cagataagca cccaagagga cgagcagcat aaatgccagt
    15181 gtgaacaggg caacagacaa cagatagatt tttcggcggt tcatggcgtt cggtcggaaa
    15241 cggtatgttc ggattatatc cgattgggac ggtattccct agagcttgga aaaatgccgt
    15301 ctgaagcggc tttcagacgg cagggcaggc ggatgttatg cctgtttgtt ccaacgttcg
    15361 atggattctt taataacttt ttttgcttct tccgcatcgc cccaggattc gactttggtc
    15421 gtaccggctt ttttcaggtc tttgtagtgg ttgaagtgga actcgatttg tttgatgagc
    15481 tgctgcggca aatcggacaa agttttgtag gcgttgccgt tgttgcggtc gtcggcggga
    15541 acgcagacga ttttgtcgtc cacttcgccg tcgtcaacga atttcatcac gccgataacg
    15601 cgcgcttcca agaatacgcc ggttgccaaa ggttgttcgg taacgagcag cacgtccaat
    15661 tcgtcgccgt cttcgtccaa agtttgggga atgaagccgt agttggtcgg tttggcgaag
    15721 atggcgggtt cgacgcggtc gagttggaat gcggccagtt tgcggttcca ctcgattttg
    15781 tggttgctgc cggcggggat ttcgttgacg acgttgatga tgccgccgtc cacatcgccg
    15841 ggggtcagga tttggttgaa gtctgccatt tggtttcctt tgtttgggaa agtttgaagt
    15901 ttgaaagtat agcacaaacg tccggctgaa aatgcgcccg atgccgtctg aaagggtgta
    15961 cgggcgcgtg ttaccgtttg cccaaaaacc tgcccagttc cagaatcgcg cgcctgttgg
    16021 acggggaaaa cactttttcc gccgcttctt ccaagccgaa ccagccgtag gagacgtgtt
    16081 cttcgggttg cagggcgatg ggcgtatcac gcgggatttc ggcagagaag aggtgttcgc
    16141 ggttttcaaa cacgcctttt ggatagcggt gccgccagtg gtggtagatt tcgtaaaccg
    16201 tgctgtcgtg ccagtcttga agctgcccgt ccgccagcag gatgccggtt tcttcccaaa
    16261 cttcgcgcct tgccgtttgg gcgacggttt cgcccggttc gaggctgccg gttaccgact
    16321 gccaaaatcc ttccggatgc gtgcgttcga tgagcaggat gccgccgtcc ccgctataaa
    16381 ggacgaccag tgcggagacg gggtatttga gcggttttgc catcggcatc tttcggcggg
    16441 ctgcggtaat gaaggggcgg attatagcaa acgccgcacg ttatggcgtt tatccttttc
    16501 cgtatccttt tttctgcacg gatgggacgc gccggtgttt gccggtaaat tttccgttgt
    16561 gtcaaaaaga taagggcggt tgtgatttta atgcttgcca aagcgtcggg cggaaactat
    16621 aatccgaaac ttatcgagtc ggagtgtggc gcagtctggt agcgcacttg catggggtgc
    16681 aaggggtcga aggttcgaat cctttcactc cgaccaaaaa ttccgaaagc cgctttcaaa
    16741 agcggctttt ttgccgtccg tatgattatg atgtagagta cgcggcgaca gacattcaaa
    16801 tgccgtctga aaaccgttca gatggcatct ctttatctta gtttcattcc gtaccatctt
    16861 aaggaacatc aaattgggca tttcccgcaa aatatccctt attctgtcca tactggcagt
    16921 gtgcctgccg atgcatgcac acgcctcaga tttggcaaac gattctttta tccggcaggt
    16981 tctcgaccgt cagcatttcg aacccgacgg gaaataccac ctattcggca gcagggggga
    17041 acttgccgag cgcagcggtc atatcggatt gggaaacata caaagccatc agttgggcaa
    17101 cctgatgatt caacaggcgg ccattaaagg aaatatcggc tacattgtcc gcttttccga
    17161 tcacgggcac gaagtccatt cccccttcga caaccatgcc tcacattccg attctgatga
    17221 agccggtagt cccgttgacg gattcagcct ttaccgcatc cattgggacg gatacgaaca
    17281 ccatcccgcc gacggctatg acgggccaca gggcggcggc tatcccgctc ccaaaggcgc
    17341 gagggatata tacagctacg acataaaagg cgttgcccaa aatatccgcc tcaacctgac
    17401 cgacaaccgc agcaccggac aacggcttgt cgaccgtttc cacaataccg gtagtatgct
    17461 gacgcaagga gtaggcgacg gattcaaacg cgccacccga tacagccccg agctggacag
    17521 atcgggcaat gccgccgaag ctttcaacgg cactgcagat atcgtcaaaa acatcatcgg
    17581 cgcggcagga gaaattgttg gcgcaggcga tgccgtgcag ggtataagcg aaggctcaaa
    17641 cattgctgtt atgcacggct tgggtctgct ttccaccgaa aacaagatgg cgcgcatcaa
    17701 cgatttggcc gatatggcgc aactcaaaga ctatgccgca gcagccatcc gcgattgggc
    17761 agtccaaaac cccaatgccg cacaaggcat agaagccgtc agcaatatct ttacggcagt
    17821 catccccgtc aaagggattg gagctgttcg gggaaaatac ggcttgggcg gcatcacggc
    17881 acatcctatc aagcggtcgc agatgggcgc gatcgcattg ccgaaaggga aatccgccgt
    17941 cagcgacaat tttgccgatg cggcatacgc caaatacccg tccccttacc attcccgaaa
    18001 tatccgttca aacttggagc agcgttacgg caaagaaaac atcacctcct caaccgtgcc
    18061 gccgtcaaac ggaaagaatg tgaaactggc aaacaaacgc cacccgaaga ccaaagtgcc
    18121 gtttgacggt aaagggtttc cgaattttga aaaagacgta aaatacgata cgagaattaa
    18181 taccgctgta ccacaagtga atcctataga tgaacccgtc tttaatccta aaggttctgt
    18241 cggatcggct cattcttggt ctataactgc cagaattcaa tacgcaaaat taccaaggca
    18301 aggtagaatc agatatatcc cacctaaaaa ttactctcct tcagcaccgc taccaaaagg
    18361 acctaataat ggatatagtg ccgggaattt aagcaaaaaa gtcctgactt tagtttacat
    18421 aaacggcaaa ctcaaattcg ggccaagcag tctgccgtct tcggcattga aagcacctgt
    18481 tcccattgtc ggcaaggctg aggctccgcc cggaaaagct gtgcctgtcg gcgaagcccg
    18541 cattccttca aatccaaaag cggtaaaagg aacaaccgtt accctgtcgc aggaacaacg
    18601 cctagccaac ggagagctga taccgaaagg ctctgtggta acgtggtcgg acaatgcggt
    18661 gaaggttgtg cggcctgatg ggaaaaagcg gagatttaat agtcgggatt gggaacagaa
    18721 ggcattgccg aaaccgcaac taccgaaaga ttacagagat gacatcaaca taggtggaaa
    18781 taatcctaga tttaatcaac aacggcatga aaaaatcgaa tcccacaacc caaacagaat
    18841 agaggagata cgccaacgta atgccgtttc ttctgcagaa aggcaggctg atgcattaaa
    18901 aaggatgcaa acgtctggcc gcacccgcct gcctgggcag ccacaaaaag gaaaacccgg
    18961 cgctgctaga gaaatgtcct cttcagacaa tccgaatcaa acagccgagg cttttgcacg
    19021 tcgatattta ggcaggaaac ctacactaat ggaacgagtc gaaggtgaaa aattatacgg
    19081 aggtaaatgt gtgggctgtt ggaaagcgca aatgccggac ggaacctttg tggtgtatag
    19141 gccagcaggt aatgcttcta tgaccaaggg aactactgcg tctgttgata ttaataaccc
    19201 aacattaaat aacttaaata ttaatttaaa aggcaagcca acagagttaa aatttaaatt
    19261 ccctgctaga taaggatgtt ttaatgaatt tagagacagc agaaaatatt caatgcgaat
    19321 ttgagtattt agcattagat ggttattttc caatgcattt tgcatcgcat ggtcaaggaa
    19381 acaaagattg gcaatttgca gttgaattca tatatagatt attaatatgt caattagcaa
    19441 ctcttgaacc tatcaacttt gaaacgaaaa atgatatttt agacttttgc cataatttag
    19501 caaaacaaag tccttttaat aatgataatg aggtgtggta tcagggggaa attgtattaa
    19561 caaaaaaagg gatagattta ataaaggaat atattccaga ggcgtttgaa gagtggaatg
    19621 gaaagaaatt tgaattaaac attcctttca ttaaaacatt aaaaaatata tttgttaatt
    19681 atgaggtagc atgggatgag aataatccac tattccccat tatatcttta aacctcggca
    19741 cctgataaac atggggttta tcaagcgaca gtggaaatta aaaagcctga tggaagttgg
    19801 gaaatgaaaa aaggaagggg cggaaaacct atgacaaatc attctatgtt cccaaaagat
    19861 tgggatgagg ctagaattag ggctgaagtt acttcggctt gggagaaaag aaaggaatta
    19921 gaaggtcaaa aatggagagg tacaagtaaa tctggtgttg aaatagaagg atttacggaa
    19981 cctaatcgaa cagcttatcc actttataat agtaaatgaa gtattgatta aaaaggtgtt
    20041 tttaaatgta taaaataaaa tgtttttatg atcagtttag ttctggtgaa ttatttaact
    20101 actgccagtt tttagataat tcttctaatc aaaagataaa tacacgagga acggtgtatc
    20161 aatatattat ttatgtttta acaggagacc tttatctaca aaaagacata gatgaaaatt
    20221 tagaatttat ccatcaagca gaaaataatc ccaatgaagt ttattcagga ggtggacaag
    20281 gtttttgctg ggatatttct gctgaaaaag tagttttcta tcacaacgaa tttgatgaag
    20341 aagacggctg gcctgattta agctgttcgc tgcatacttt taaaactgct ttaattgctt
    20401 ggaatgcttt tttgcaattg cctaaaagta ttcattcggt ggtggagact gtgattgagg
    20461 aataagcata attagcttaa tgaatagaat cagcgatata gattggactg caaatccacg
    20521 cttatacgct gtgccatgat taacaggaag agagcaactt ggatgggcta gtagggatgg
    20581 taagcattta aatatatcaa ttgatggaaa gattacacac aaatgactaa tttaaaatta
    20641 gatttctact ctgaagttat tataaaagat tcttgcccta atgatttgtt agaaaatggt
    20701 gagactatta aaggaaaaaa aggagtggta ttaggtataa gcgaagaaga tggtataatc
    20761 tatggctata ctattttact ttttgatata aaatattgta gatatataga taagaaatat
    20821 attataccga ctggaaaaaa attctctcgc gatgattttt attagctaat ttaagtaatt
    20881 taagcaagtt ttttagatac tgaaactggt aattatgttt atgtaagcat aaggagttca
    20941 gattgaagga ttctacaggg caaatgaggc tggcaaccaa ggatttggcg gaagccatta
    21001 aacgaggaga agtacgtagt tctgctttta caataaagca actaaaggca attgaaaaag
    21061 gagaatctaa aataccggat tatacttggc atcatcatca agacactgga aggatgcaat
    21121 tgattaatga agacttgcat catgataccg gccatatcgg ttggagagcg atgagtaaag
    21181 gaaagtaact atgtggaaaa tcataaaaga ggatagtgat gatttaggat ttgcaattaa
    21241 atgcttattc tctcagtcta ttgatttaaa tgaattcaag ttatggattg aacaagtaat
    21301 acgcgatatg cccat
//
LOCUS       DAWGJV010000018        20707 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWGJV010000018 DAWGJV010000000
VERSION     DAWGJV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.018"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(63..239)
                     /locus_tag="WHQ94_000486"
     CDS             complement(63..239)
                     /locus_tag="WHQ94_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127818.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WHQ94_000487"
     CDS             complement(242..1600)
                     /locus_tag="WHQ94_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ2127819.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMVAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALINNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1724..3370)
                     /locus_tag="WHQ94_000488"
     CDS             complement(1724..3370)
                     /locus_tag="WHQ94_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ2127820.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYVAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3393..4871)
                     /locus_tag="WHQ94_000489"
     CDS             complement(3393..4871)
                     /locus_tag="WHQ94_000489"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ2127821.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTLWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4896..6641)
                     /locus_tag="WHQ94_000490"
     CDS             complement(4896..6641)
                     /locus_tag="WHQ94_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ2127822.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="WHQ94_000491"
     CDS             complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="WHQ94_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ2127823.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="WHQ94_000492"
     CDS             complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="WHQ94_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ2127824.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="WHQ94_000493"
     CDS             complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="WHQ94_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ2127825.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8679..9896
                     /locus_tag="WHQ94_000494"
     CDS             8679..9896
                     /locus_tag="WHQ94_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2127826.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKDEGGSFEHVGVCTDNGRFAY
                     SARPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            10028..10104
                     /locus_tag="WHQ94_000495"
     tRNA            10028..10104
                     /locus_tag="WHQ94_000495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10062..10064,aa:Met,seq:cat)
     gene            10112..10187
                     /locus_tag="WHQ94_000496"
     tRNA            10112..10187
                     /locus_tag="WHQ94_000496"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10145..10147,aa:Ala,seq:ggc)
     gene            complement(10478..11299)
                     /locus_tag="WHQ94_000497"
     CDS             complement(10478..11299)
                     /locus_tag="WHQ94_000497"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ2127827.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLVGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGIAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11333..11977)
                     /locus_tag="WHQ94_000498"
     CDS             complement(11333..11977)
                     /locus_tag="WHQ94_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2127828.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVVVGGKYRVIFNNGFGGGVHTIKELVAKVRAERKLSNHPVQK"
     gene            complement(11980..12843)
                     /gene="ftsN"
                     /locus_tag="WHQ94_000499"
     CDS             complement(11980..12843)
                     /gene="ftsN"
                     /locus_tag="WHQ94_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ2127829.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12949..14445)
                     /locus_tag="WHQ94_000500"
     CDS             complement(12949..14445)
                     /locus_tag="WHQ94_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ2127830.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSANVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14459..14779)
                     /locus_tag="WHQ94_000501"
     CDS             complement(14459..14779)
                     /locus_tag="WHQ94_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ2127831.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15262..16788
                     /gene="putP"
                     /locus_tag="WHQ94_000502"
     CDS             15262..16788
                     /gene="putP"
                     /locus_tag="WHQ94_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ2127832.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDQVASMKGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17039..20644
                     /gene="putA"
                     /locus_tag="WHQ94_000503"
     CDS             17039..20644
                     /gene="putA"
                     /locus_tag="WHQ94_000503"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ2127833.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNT
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDTKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETVGISHVAALSP
                     LNSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4847 a         5993 c         5063 g         4804 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttac cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtccgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg gcttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcaaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcatacgc accgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca tatcgcccat tacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc cgggttcgca ttataagtat
      601 cgtcaatcag ggtcgcgccc ttgatgccgg ctttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtca aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgatca aagcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatgc ccatttcgat gacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttgcc gcccgaaccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccac catttgcaac gcggtaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt ttttatcgcc tgttttactg tggtaaaaca
     1681 cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt ttagacgtat
     1741 ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga
     1801 taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa aaatcatagc
     1861 cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat
     1921 taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac gaaattttaa
     1981 ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc
     2041 ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag ctttctttat
     2101 tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa
     2161 catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta tccaaacgtg
     2221 tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg
     2281 catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc aaccccgcgc
     2341 ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg gcataggtgg
     2401 aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc gtccgaaaac
     2461 ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc cccaaagtct
     2521 gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc aacagccctt
     2581 tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc atataatccg
     2641 aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca acatgacgga
     2701 tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg gcgacatatt
     2761 gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac aggccggcat
     2821 caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag gacaaaatca
     2881 aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt acgtcagcaa
     2941 ataccccccc cgatacacaa tatttccaaa ctgtcaagcc aaaaatgaaa aataatgcct
     3001 gcacaaaata aagcgacccg ggaagattgc cgacaaattc acgcgcctcg gcaggattgc
     3061 tttccaatat cgaaccgact atctgataag acggcgcacc atacaaccag ccgaccggca
     3121 aatatagggc ggttgtgccg acgtaaatca gtaaaacaac tgaagaaacg cgcgggaaac
     3181 cgcgtgccaa taataataaa aatacaaccg aagccaaagc acccacggca gaataacggt
     3241 agccgtattc atattccaag tgataacctg atgcgatggc cgcgccaaaa caaaaggcta
     3301 aaagcgcgca aatgcccaat gtatttttta atgtctgatc catatttttt attcgggcgg
     3361 attgtttcat acgatgccgt ccgaatatcc cattatttac gagttaacaa agcctgtccg
     3421 acgatttcaa gatcggaaaa acggtgcttc acgccttgta catcctgata gttttcatgc
     3481 cctttgccgg caatcaggat gatgtcgttt gcggcggctt gttcaaccgc ataacggatg
     3541 gcggcggcac ggtcggcttc gacgcattcg ggcgcgggaa cggcaggcag gatgtcgttg
     3601 atgatgtcgt gcggattttc caaacgcgga ttgtcgctgg tgacgacgac tttatccgcg
     3661 ccctgtacgg ctgccgcgcc catcagcggg cgtttgccgc gatcgcggtt gccgccgcaa
     3721 ccgaatacgc accacaaagc cgcaccctgc ggtttgattt cctgcaaggt ggcgagtgct
     3781 ttttccaatg cgtcgggcgt atgggcataa tcgacaacga ccaaaggttt gccgctgttc
     3841 atgatgcagt ccatgcgccc tgaagcggga cggatttttg ccagcacatc caatacatta
     3901 tcaagcggat agccgttggc gcacagcaag gcgatgcagg cggcgaggtt ttgcgcgttg
     3961 aaccgtccga gcaggcgcgt gcggcatttc ccttcgcccc acagggtttg gaatacggct
     4021 gctatgccgt ctgaagaggc ggtaaagtcg gtaatgcgga tgtcggcgtg ttcgctgaag
     4081 ccgtagctat aaacggccaa atcgggacaa tcttttttca gacgacctgc gagttccgcg
     4141 ccgtattcgt catctacatt gataacggcg tgtttcaagc cgtgccagta aaacaggcgc
     4201 gacttgatgg caccgtaggc ttccatcgtg ccgtggtagt cgaggtggtc gcgggtgagg
     4261 ttggtaaaga ttgcgctgcg gaatgacacg ccgttgacgc gcgactggtc aagcccgtgg
     4321 ctggagactt ccatcgcggc gactgttgcg ccttgttgac ggaaacggta gagcagggtt
     4381 tggacatcga cgggggcggg tgtggtatgc gtggtttctt ccaatgcacc ccaaaagccg
     4441 ttgccgaccg tgccgacaat ggcggttttt tcgcccaaca aatcggcagc ttgcgccagc
     4501 cattgcgtga tggaggtttt gccgttggtt ccggttacgc cccagacttt gagaccgtct
     4561 gaaacgttgc cgtaaacttg cgccgccaat atgccggcac ggtgtttcaa atctttgatg
     4621 ccttgattgg ggactttcca ttcgggattc cacgcgaatt tgccatcgtc gtcccaaaaa
     4681 acaaaagccg cgccgttggc aacggcggcg gggatataac tgcgtccgtc ggcatattcg
     4741 cccggacagg caacgaaaat atcgccttgt ttgatttggc ggctgtctga atgcaacaaa
     4801 cgccctgccg cgtttgcaca cgacaaggtc gggatgccgg tttcagccaa aggggttaac
     4861 ttgctgaaca taaaacaatc tcgttgatac tcggattaag acggtgtttt gacggctgcg
     4921 gcggtcagtg gcttggtcgg ggaaatgccc aagatgttca ggctgccgcc cataattttt
     4981 ttgaagggcg gccctgccac tacgccgccg taataaccgt gggcagtcgg ttcgtcaatg
     5041 gttaccgcca caatcacacg ggggtttttg gcgggggcaa aaccgataaa ggtagcgatg
     5101 tgtttgttgt cggcataacg cccgttgacg aacttgcgcg ccgtgccggt tttcgcgccg
     5161 acatcgaaac cgtccaccgc acccgccgta ccggtgccgc ccggctcggt cacggaaacc
     5221 atcagattgc gtacctcgcg cgcggtcgat tctttgaata tgcgtttgcc ttgcggcgca
     5281 accgcctgtt tttcaaagct gaccggcagc aaaacgccgt cgtgcgtcag tgcggtatag
     5341 gcgcgcgcca attgcagcag gctcaattgc aggccgtaac cgaaagacat cgtcgcctgt
     5401 tcgatgggcc gccacctgcg ccaatttctc aacaaacctg cagtttcgcc cggaaagccc
     5461 gagtgcatac gcacaccgat gcccaactca tgatagaagt catacatttc ttcggcaccg
     5521 aaacgcgcag acagtttgct tgtgccgacg ttggacgatt tctgcatgat gccgcgcaca
     5581 tccaaagagg ggtaaacatg ggtatcgcgc acgggagacg gtccgatttt ataaggctgc
     5641 gtattcagcc gttcgttcaa atcggttttg cccgcatcca atgctttcgc aatcacaaac
     5701 ggtttgattg ccgaaccggg ttcgatcata tcggttacgg cacggttgcg ccgctgttcg
     5761 ctgtctgccc ggccgggcct gttgggatcg taggcgggcg tattggccaa ggcgaggatt
     5821 tcccccgtgc gggcatccaa aaccaccacc gttccggctt ttgcctgatg gtattcgacc
     5881 gccttgttca actcttcata ggccaaggtc tgaatcctct gatcgaggga aaggatgatg
     5941 tctttgccgt ttttcggggc tttattgcgc ggggagtcca agctgtccac aatattgccc
     6001 tgccggtccc gcaaaacgac ttccgcgccg tcttcgccat gcaggctgtc ttcaagcgaa
     6061 agttccaaac cttcctgacc tttgccgtca atatcggtaa atccgatgac gtgtgcaaac
     6121 aggttgccca tcgggtaatg gcgttttaat tctttttcaa atacaaagtt ttccaaaccc
     6181 aaggctttga cctcttcggc aaccttggga tcgagctgcc gcttaatcca gataaacgac
     6241 ttgcctttct gttcgagctt gttcctcaaa acatcaaccg gcacatcgac aagctcggac
     6301 aggcgttcca attgtgcggc agacggcatt tccttcatct ctttaggcac ggcaaacagg
     6361 gactccgtcg gcgcactcaa cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt
     6421 gtagccggca atgtttgagt ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta
     6481 tacgttaccg tctgcagata cagtccgcgg gcaatcagac cggcaaacaa gaccgctatt
     6541 gccatcagga cgaagctgat ccgtccgtta ctggtcatcg gctttttgac ctgctcttct
     6601 ttgggcagca tccggggctt atattcactc ttaatcaaca tttttacttc tcgttattat
     6661 tatcctgacg caggaatccg attccggcac acaggctgct tctatctttg atgcgccacc
     6721 ataaaggtat tgcccgaaac cggcggatgg aggttttgtt tttctgccgc cgccctgatc
     6781 gcttcgtggt tcgccaaacg cgcctgttgc agcctcattt gcgcataatc ctgctccaag
     6841 gcgatttcct gttttttcgc cttatccaaa gctgtgaagt tgagcctgta ctggttttgc
     6901 tgcatcacaa cggaaaaagc ggaaacgcac accgcaagca gcagaaggaa attcaatttg
     6961 ttcattgcca ttcagacgtt tttctctgtg attgttccgg tatcggaccg gcagtccgct
     7021 ccgccacacg caaaaccgca cttctcgccc tcggattggc ggcaatttcc gcctcacccg
     7081 gctttaatgc cctgcccgcg attttcaggg gcggctcggg caaatccgct tccctgaccg
     7141 ccgcccagcg cggcaggggc gcgtgttgcg aatatttttt gacaaactgc ttcacaatgc
     7201 ggtcttccaa cgaatggaaa gcaatgaccg ccaaacgtcc gccctctttc agacgacaca
     7261 tgacctgcgg caatactgcc cctacttctt caagctcgcg gttaataaag atgcggattg
     7321 cctggaaggt gcgcgtcgca ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca
     7381 cgatctgcgc cagcttgcgg gttgtatcga ttggactttc cgcccgttgc gcaacaatgg
     7441 cgcgcgcaat ctggcggcta aaccgctctt caccataatt cttgattacc tcgtgcaaat
     7501 cctgttccga cgcaaccgct atccactctg cggcagacat accgcgcgtc gtatccatac
     7561 gcatatccaa aggggcatcg aaacggaagc tgaaaccgcg gctgccgtca tcgatttgcg
     7621 gggacgaaat ccccaaatca aacagcgcac cgtccacctt gccgataccc aaaccgtcca
     7681 atgccgtctg aaacgaagca aaaccgccat gcacgacacc gacccgtttg tccgaacgcg
     7741 ccagctcttc tgccacagca atcgcctgcg gatctttgtc gaaaacaatc aaccgccccg
     7801 catcgcccaa acgcgacaaa atcagccggg aatgccctcc cctgccgaac gtaccgtcca
     7861 catagacacc gtcttcgcgc acggcaagcg catccaccgc ctcattcagc aagaccgtga
     7921 tatgccggta actttctgct ccactcacaa ttgcaaatcc gtctgaccca actggaaggc
     7981 aagttcgtca ggatcgtcat ccaaagcctg aaccatctca gcctcccact gctcgcgacc
     8041 ccaaagctcc aaacggttgg cacgaccgac caaaacgact tcacggtcga aatccaccct
     8101 tttcctcagt ccggcaggaa gcagcacccg gccggcgctg tcccattcca aaatttccgc
     8161 gttatgcagc aaaagatttt gaaaccgccg caaaacaggg ttatccgcca cttttaagtt
     8221 taaaagttgc gccgcaacct tttcccactc cgcaacaggg tacatcaaca gcttgtgttt
     8281 cgactcgagc gttaccacta cggcaggcgt atagaggcgc gacagaatgt cacggaattt
     8341 ggcaggaaca gccaaccgcc ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat
     8401 gacatgtccc aagccgaaat caaaatcaca agggtaaaag agacactttg ccccacaatt
     8461 ccccaccaat cgacactata agaaatttta aacactcggt caaatcaggg catgaaaact
     8521 cattaacata tctgaaattt ttattccttt taaaaacaat aagataaaaa atgacgacaa
     8581 cggcacggcg ggtgcggtac agaataatcg aaccaataaa caactatata tgattaattt
     8641 aataatataa acacaatata tagtattaag ataaagccat gacagcaccc gtaccaacgt
     8701 gtaatatgtc gggaaatcca ataaatttac acaagctaac acttatcatg cccctccccc
     8761 tcccctctcc cgaagcacgg caattctcgc tcaaactgca aaccctcatt gccgaaaaaa
     8821 tcggcaaaca cggcaactgg attccatttt cacgttttat ggaattggtt ttatacgctc
     8881 cgcaatacgg ctactacacc ggcggcagcc ataaaatcgg caataccggg gattttatta
     8941 ccgcaccgac cctcacctct ctgtttgcac agacactggc acgccaactt caagaacttc
     9001 tatcccaaac ggcgggcaat atctatgaat tcggcgcggg aaccggacag cttgccgccg
     9061 atttgttggg cagcatttcg gacggcatca gccgttacta tattattgaa atatcgccgg
     9121 agctggcagc acgtcagaaa aacctgattc aagcccgcgc accggaagca tctcaaaaag
     9181 ttgtccactt gaccgcactt cccgaagcgt ttgacggcat catcatcggc aacgaagtac
     9241 tcgatgccat gcctgtcgaa atcgtccgta aagatgaagg cggctcattc gagcatgtcg
     9301 gcgtttgcac ggacaacggc cgtttcgcct attctgcaag acccttgcac gacccgtcgc
     9361 tttccacctc ggcttccctc tattttcctc aaacagatta tccctacacc agcgaactac
     9421 atccgcaaca atatgccttt atccgcaccc ttgcctcaag actcgaacac ggctgcatga
     9481 tattcatcga ctacggtttt gatgcagcgc agtattacca cccgcaacgc aatcaaggta
     9541 ctctaatcgg acactaccga catcacgtta tccacaatcc ttttgacttc atcggattgg
     9601 ccgacctgac cgcgcatgtc aactttaccg acattgccca agccgggacg gatgccggat
     9661 tagatttgat aggttacctt ccccaatccc atttcttatt gaacctgggc attaccgagc
     9721 tattggcaca gacggggaaa acgaattcgg cagcctatat ccgtgaagct gctgccgttc
     9781 agaaactgat tgaccagcac gaaatgggcg aactgtttaa agtcatcgca ttcggaaaaa
     9841 atatcggcat cgactgggca ggattccgct tcggcgacat ctgccacaaa ctctaaccct
     9901 catgccgcct gaatccgctt cagacgacat aaacttttta acatttaaaa acagtcaact
     9961 aattcaaaat taaaaaatac ggcttgtcaa aaaaacagaa aaacatataa tagcgtcttc
    10021 acgaaacggc gaattagctc agtcggttag agcagaggaa tcataatcct tgtgtccggg
    10081 gttcgagtcc ctgattcgcc accaaatttt cgggggtata gctcagttgg tagagcgctt
    10141 gcatggcatg caagaggtca gcggttcgat cccgcttacc tccaccagat aaaaaagcac
    10201 agaccgtaaa aaggtatgtg cttttttatt acctgattac cggcaaataa agaataaacc
    10261 actgccttca aaacaggcaa tcgactttaa acctatcgcc ccgcctgtcc tgattttata
    10321 gtgaattaaa tttaaaccgg tacagcgttg gctcgccttg ccgtactatc tgtactgtct
    10381 gcggcttcgt cgccttgtcc tgatttttgt taattcacta tatcagcccg ccagacaaac
    10441 ccgacccgaa taatgtcttc aggtcgggtt tatggtttca ttcccaactt atccagcccg
    10501 acaaccacaa tattatgatg acgatgccga aaacaatgcg gtaataggca aacgggatat
    10561 agtttttctt ggaaacaaac ttcagcagtg ctttgaccgc tactaaaccg gaaacaaaag
    10621 cggcaataaa gcctatcaga atcaaaccga catcatgcag ggtgaaaaac cggtaatgtt
    10681 tcaggacatc ataagccgtt gcggcaacca tcatcggaac ggccaagaaa aatgaaaact
    10741 ccgttgccgt tttccgctcg attccccaaa gcatcccgcc cataatcgta ctgcccgagc
    10801 gggatgttcc cggaatcaag gcaaacacct gcgcgatacc gatcatcaag gcatcaatcg
    10861 gacgcaatgc atcaacatcg gcaattttag gctctgctcg gctttggcgt ttctccaccc
    10921 acaaaataaa aaaaccgccc aaaaccagca tgactgcaac actcaagggg ttaaacagat
    10981 actctttgat ttgtttgccg aacaacagcc ccatcacggc ggcaggtata aaagcaatgg
    11041 caagattgag gacgaaacgg ttggctttcc ggtcttttcc taagccgtgc aacacattgc
    11101 tgaaacgctg ccggtattca aacactactg ccaaaaccgc accaagctgg atggcaattt
    11161 caaaaacctt gtggttgctg tgaaaaccga ccaagttacc gaacacaatc aaatgtccgg
    11221 tgctggaaat aggtaaaaat tcggtaaaac cttctaccaa gcccatcatc agggctttca
    11281 ggacaatcag aaaatccatt gcttggtttc tttcaaatgc gcggggatgc ggttattttt
    11341 gcacaggatg gtttgacagt ttgcgctccg ccctgacttt ggcaaccaat tctttaatcg
    11401 tatgaacgcc gccgccaaag ccgttattga agataacgcg gtattttccg ccgacgacaa
    11461 cggtcggcgt gctgtcaata ccgtattgtt ccgtcagttt ctgcattttt aatgcggcgg
    11521 cggcagcttc gggggaatca taggcgcgca tcagtttttt gccgtcaaag cctttttgag
    11581 acaaagccca ttttccggca acagccctgt tttccaagcg gattttttgt tcgtaaactg
    11641 ctttaaacac agcagggttt gcctgatatt tcaaacccga caaattgacg gcagccgcca
    11701 tcctggccag accgagcatt tcaggctgcc agaccacgtg ctccgtcctc aaataggcat
    11761 cagacggcaa tgccttgccc aatttcaata acaaaggatc gaaatgatgg caatgtacgc
    11821 agaaatagcc gaaaaattcc aaaacctcaa ttttgcccga ctgttcttga ggaatgggtt
    11881 tatccaacac aagatagtct tccctttccg tcagggcata tgcctgcgcg gacaacactg
    11941 ccgacagcag cagcggcaac agatgtttga gcttcataat tatttgcttt cgatagaacg
    12001 gatcaggctg gcgacttcat gttttttcaa ctcgtcctgc atttttttca ccgcatcggc
    12061 agacatattg ccgctttgca cccggtaaag cgttttatgt cccgcctgat aaccgaccac
    12121 cttggaagat atgcccaaga ttgccagttt ggcacgctgt ccttccgcgc tccggcggtc
    12181 ggcatacgcg cccatttgca gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt
    12241 ctgcacttct ttggcagcgg cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg
    12301 ttccggggtc ggtttgggtg caactttttc cttctccgcc tttttctctt cttttgaagc
    12361 ttttttctct gtttctttag atggttttac cgcttgtttt ttaaccgttt cggcatcttt
    12421 cttctgcgct ttttccccga cggtttgttc acgctcttcc gtcagtgctt tcttgcgcac
    12481 tgcctgtccg tccgactttt cccgctccgg ctcgtccgcc ttttcctcaa cctcgtcggc
    12541 tttgtcggca acgggctgct tgtcggcagc tttttccgca tccgactgct ctgcctcttt
    12601 cgcagcatcc ggttcggaca aggcgttttg atcggccggt tcaggttgga tgtcttcctt
    12661 aggctggttt ttcggtttca ggatttccgt ttctgcaggc tgcttcgacg gaaccgggat
    12721 tttgaacgca ttttgaccgc tctggttcag ataaaacaaa ataccggcaa taatgaccgt
    12781 cgccagtatc aaaccgaaga aaaaaccgga cagacctttt ccggattggg aaaatttgtt
    12841 cataaacata ccttaatgtg tttcagacgg cattaacgcc gtttgcttac gggcggatat
    12901 tctaacaata tcgccatatt tgggcaaaac ctgcttccat tcccgttcct ataaagcgcg
    12961 acggaaacct atggctttca tgacgtggct tctgccgact tcttcgtcgc ccgccaaatc
    13021 cgccaatgta cgcgccacgc gcataatgcg gtggaaactg cgggcggaga gggagagttt
    13081 ttccaacagt ccgcccaagg cttcctgcgc ttctttttgg atgcgggcgg acgtgtcgag
    13141 ttcgctgacg ctcaaggcgg cattcacttt gccctgccgt gcgtattgtt tgtcacgggc
    13201 ggcgatgacg cgttccaaaa cgtttgcact gctttcccct gcttcctgct gcatcagttc
    13261 ggcggcggac aggctgggga cttcgatggt caaatcgatg cggtcaagca gcgggccgga
    13321 aattttgctg cggtaacgcg cgacgctttc gggcgtgcag cggcagggtt tgacgggatg
    13381 cccgagataa ccgcacggac acgggttcat ggcggcgaca agttggaatt tggcaggata
    13441 gacggcttgg cgcgccgcgc gggaaatgtg gatttcgccg ttttccaacg gttcgcgcaa
    13501 aacttccaaa actttgcggt cgaactcggg caattcgtcc aaaaacaaaa caccgtgatg
    13561 cgccaatgaa atctcgcccg gacgcggatc cgaaccgccg ccgaccatag ccgccgcgct
    13621 ggcgctgtga tgcggactgc ggaaaggacg gttgctgtcg agttgttgct ggtggttggg
    13681 caggagcgaa cgcaatgccc aaacttcaac caattcgtct tcggtcagcg gcggcaggat
    13741 gccgggcagc cgttgggaga gcatagactt gcccgttccc ggcggaccca tcatcaagag
    13801 gctgtgtccg cctgcggcag cgatttccaa agcaaggcgg gcagtgtgct gacccttgac
    13861 atcgacgagg tcgagcaatt tggtgttttc agacggcatc tgaggaactt gacagtcggt
    13921 ttgcaccaaa ggttcgatgc cgttcaaatg ggcggcgact tcgcccaaag agcgcgcgcc
    13981 gtaaacggta atgccgcgca tcacggcggc ttgtcctgcg ttttcttcag gcaaaacaaa
    14041 tgcacgtttt gcctgcatac cctgccacgc catcgccaac gcgccgcgca cggggcgcaa
    14101 cagccccgac agtgccaatt cccccgcaaa ctcgtattcc gccagttttt cgggcgcaac
    14161 ctgccccgat gcggcaagga tgccgattgc aatcggcaaa tcgaaacgcc ccgactcttt
    14221 gggcaggtcg gcgggggcga ggttgacggt aatttttttg gcggggaatt caaaaccgct
    14281 ttgaataatg gcggcacgga cgcggtcgcg actttccttt acttccgtat cgggcagccc
    14341 gacgatgttg aaatgcggca ggccgttggc aaggtgggct tccacttcga ccaacggcgc
    14401 attcataccg ctcaaggcgc ggctgtaaac caaggcaagc gacatatttc agacggcctt
    14461 attcgccggc ttcggtttgc tgcctgattt cggcgacggc ttcttcggca gcggcttcag
    14521 ccgcttccaa tgctgcccgt tcgggatttt gcgcggcttc gagttttgcc aaacgcgctt
    14581 ccaaagccgc cagtttggta cgagttttga ttaaaacctg ctgctggatg tcgaattctt
    14641 cgcgcgtaac caaatccata cggttgaacg cgccgcccag catcgcctta atattttttt
    14701 ccacatcttt ggcagggctg ttggcgatgg tttcgctgat tttcgaaccg acttcttcaa
    14761 aaagctgctt gccgaacata atctgtatcc ttcctgaaca tatcaaattc aatcggctat
    14821 tgtataagga aaaatgccgt ctgaaaacgg gcggcggata atcggcaaaa cataccgcgc
    14881 ctcctttgcg gttgctaaat tacacctcaa aacacctgcc gcctgaaaac ggatttcaga
    14941 ttccgcgcaa cgcctgatta caagacacta caaaacaata tgctgtttta aatgattttt
    15001 ccgacgcgca tcgtttcaaa ctcggctttt taagccatta agtgctttgc aaacaacaga
    15061 aattgggtta tgctgaaacg gattatttac aatttcatat agttttatta catatcttat
    15121 tgtgattgaa gataatttat ccgaatcccc ctttcgggta tccggatttt ccgttgtact
    15181 tttattagaa aaacatttca ggcgcaagtt gcttgcaatt tcaaagcctg taattctgaa
    15241 gttttagacg aggaatagca catgaatccc atgtacatta cttttgcaat ctatctggtt
    15301 gcagtccttc tcatcgggct tgccgcctat ttttccacgc gcaatttcga cgattatatt
    15361 ttgggcgggc gcagcctagg cccgtttgtt accgcgatgt cggcaggcgc gtccgatatg
    15421 tccggctggc tgctgatggg tctgccgggc gcgatttacc tgagcggttt gaatgaggct
    15481 tggattgcca tcggcctctt ggtcggcgcg tatttcaact ggctcttggt ggcgggccgc
    15541 ctgcgcgtgc ataccgaata cgccaacaat gcgctgacgc tgccggatta tttcttccac
    15601 cgcttcggcg cgggcggaca cttgatgaaa gtggtttccg cactgattat cctgttcttc
    15661 ttcacgattt attgcgcctc gggcattgtg gcgggcgcaa ccctgttcca aagcctgttt
    15721 gaaggtatga cttacaatca ggcaatgtgg ctgggcgcgg gcgcgaccat cgcctatacc
    15781 ttcttgggcg gtttcttggc ggtcagctgg acggatacgt tgcaggcttc tttgatgatt
    15841 ttcgcgctga ttttaacgcc tgtgatggtc tatctgggct tgggcggcgc ggaacagatg
    15901 tctgccgcga ttcaaagcgt cgccgcaggc acgggcaaag aatacggcag cctgtttgcc
    15961 ggtacgaccg tcatcggcat catttccacc gccgcatggg gcttgggcta tttcggacag
    16021 ccgcacattt tggcgcgctt tatggcggcc gaaagcgcga aatcgctggt atccgcacgc
    16081 cgcatcggta tgacttggat ggcgttgtgc cttgcgggcg cggtggcggt cggttatttc
    16141 ggcattgcgt atttcggtgc caatcccgac caagtcgctt caatgaaggg caaccacgaa
    16201 cgcatcttca tcgcgctttc tactttgctg ttcaaccctt ggattgcggg catcatcctg
    16261 agcgcgattc ttgccgccgt gatgtccaca ctgtcatgcc agcttttggt ttgctccagt
    16321 gcgattaccg aagacttcta caaaggcttt ttgcgtaaaa acgcgcaaca gtcggaactg
    16381 gtatgggtcg gccgcctgat ggtcttggcg attgccgtga tttccatcct gattgcttcc
    16441 gatccgaaca gcaaagtatt gggactggtg tcttatgcgt gggcaggttt cggtgcggca
    16501 ttcggcccga ttgtgattct gtccgtattg tggaaacgca tcactgccta cggcgcgttg
    16561 tcgggtatgg ttgcaggtgc gttaaccgta gtcgtatggg cggaatgggt gaaaaaaccg
    16621 gctcaagcgg caggcgaaag cggcttgttg acgatgtacg aaatcgtccc gggctttatt
    16681 gtttgcttga tcgttgccgt attggtttcc ctgttcaaca aagaaccttc gcgcgaaacc
    16741 caagaacgct ttgaaaaagc cgacgcagat taccgcgccg cccgataaac ccctttcaga
    16801 cggcacggca tcccggttcg tgccgtctga acccgtttcc gggtcaaaca caaataaacc
    16861 ttgcctgcat acaggcctta tttgtgtttt acccgtttca ttttcaaaga aacggaagca
    16921 cctaccctgc ccgcatttga agccttgtat ttcatctgcg gatttattac gatattaccg
    16981 tattcagacc gcaccgatgc cgtctgaacc ccccgaaaaa ctttggagaa tccaaaaaat
    17041 gtttcatttt gcatttccgg cacaaactgc cctgcgccaa gcgataaccg atgcctaccg
    17101 ccgtaatgaa atcgaagccg tacaggatat gttgcaacgt gcacagatga gcgacgaaga
    17161 gcgcaacgcc gcctccgagc ttgcccgccg tttggttacc caagtccgcg ccggccgcac
    17221 caaagccggc ggcgtggatg cgctgatgca cgagttttca ctctccagcg aagaaggcat
    17281 cgcgctgatg tgtctggcag aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg
    17341 cctgattgcc gacaagattt cagacggcaa ctggaaaagc catttgaaca acagcccttc
    17401 cctcttcgtc aacgccgccg cctggggcct gctgattacc ggcaaactga ccgccacaaa
    17461 cgacagacaa atgagttccg cactcagccg cctgatcagc aaaggcggcg caccgctcat
    17521 ccgccaaggc gtaaattacg ccatgcgcct gttgggcaaa cagttcgtaa ccggacaaac
    17581 cattgaagaa gccctgcaaa acggcaaaga acgcgaaaaa atgggctacc gcttctcctt
    17641 cgatatgttg ggcgaagccg cctacaccca agccgatgcc gaccgctact accgcgacta
    17701 tgtcgaagcc atccacgcca tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa
    17761 cggcatttcc gtcaaactct ccgccataca tccgcgctac tcgcgcgccc aacacggccg
    17821 tgtgatgggc gaactgttgc cgcgcctgaa agagctgttc cttttgggta aaaaatacga
    17881 tatcggtatc aacatcgatg ccgaagaagc caaccgtctg gagctgtctt tggatttgat
    17941 ggaggcactc gtttccgatc ccgatttggc aggctacaaa ggcatcggtt tcgttgtcca
    18001 agcctaccaa aaacgttgtc cgttcgttat cgactacctg atcgaccttg cccgccgcaa
    18061 caaccaaaaa ctaatgatcc gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg
    18121 ggcgcaagtg gacggcttga acggctatcc gacctacacc cgcaaagtcc acaccgacat
    18181 ctcctacctc gcctgcgcgc gcaaactgct ttccgcgcaa gacgcggtat ttccgcaatt
    18241 cgccacccac aacgcctaca ccttgggcgc aatctaccaa atgggcaaag gcaaagactt
    18301 tgaacaccaa tgtctgcacg gcatgggcga aaccctgtac gaccaagtcg tcggcccgca
    18361 aaacttaggc cgccgcgtgc gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc
    18421 ctacttggtg cgccgcctgt tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt
    18481 cgatgaaaac atcagcatcg acacgctcat ccgcagcccg ttcgacacca tcgccgaaca
    18541 aggcatccac ctgcacaaca ccctgccgct gccgcgcgat ttgtacggca aatgccgtct
    18601 gaactcgcaa ggcgtggact tgagcaacga aaacgtattg cagcagcttc aagaacagat
    18661 gaacaaagcc gccgcgcaag acttccacgc cgcatccatc gtcaacggca aagcccgcga
    18721 tgtcggcgaa gcgcaaccga ttaaaaaccc tgccgaccac gacgacatcg tcggcacagt
    18781 cagctttgcc gatgccgcgc ttgcccaaga agcggttggc gcagccgttg ccgcgttccc
    18841 cgaatggagt gcgacaccag ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct
    18901 gctcgaacag cacacccccg cgctgatgat gcttgccgtg cgcgaagcgg gcaaaacgct
    18961 gaacaacgcc attgccgaag tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga
    19021 agccgaacac accctgcctc aagacacaaa agccgtcggc gcgattgtcg ccatcagccc
    19081 gtggaacttc ccgctcgcca tctttaccgg cgaagtcgtg tccgcattgg cggcaggcaa
    19141 caccgtcatc gccaaacccg ccgaacaaac cagcctgatt gccggttatg ccgtttccct
    19201 catgcacgaa gccggcatcc cgacttccgc cctgcaactc gtcctcggcg caggcgacgt
    19261 gggtgcggca ttgaccaacg atgcccgcat cggcggcgta attttcaccg gctcgaccga
    19321 agtggcgcgc ctgatcaaca aagcccttgc caaacgcaac gacaatcccg tcctgattgc
    19381 cgaaaccggc ggacaaaacg ccatgattgt cgattccacc gcacttgccg aacaagtctg
    19441 cgccgacgta ttgaactccg ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat
    19501 cttgtgcgtc caagaagacg ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga
    19561 cgaactcgtc gtcggcaaac cgattcaact caccaccgat gtcggccccg tcatcgatgc
    19621 cgaagcgcag caaaacctgt tgaaccacat caacaaaatg aaaggtgttg ccaagtccta
    19681 ccacgaagtc aaaaccgccg ccgatgtcga ttccaaaaaa tccacgttcg ttcgccccat
    19741 cctgtttgaa ttgaacaacc tcaacgaact gcaacgcgaa gtcttcggcc ccgtcctgca
    19801 cgtcgtccgc taccgcgccg acgaactcga cagcgtcatc gaccaaatca acagcaaagg
    19861 ctacgccctg acccacggcg tacacagccg catcgaaggc acggtacgcc acatccgcag
    19921 ccgcatcgaa gccggcaacg tttacgtcaa ccgcaacatc gtcggcgcag tcgtcggcgt
    19981 acagcccttc ggcggacacg gtctgtccgg cacaggtccc aaagcaggcg gttcattcta
    20041 cctgcaaaaa ctgacccgcg ccggcgaatg ggttgccccg accctgagcc aaatcggaca
    20101 ggcggacgaa gccgcactca aacgcctcga agcactggtt cacaaactgc cgttcaacgc
    20161 cgaagagaaa aaagccgcag cggccgcttt gggacacgcc cgcatccgca ccctgcgccg
    20221 tgccgaaacc gtccttaccg gaccgaccgg cgagcgcaac agcatctcat ggcacgcgcc
    20281 caaacgcgtt tggatacacg gcggcagcac ggttcaagcc tttgccgcac tgaccgaact
    20341 tgccgcctcc ggcatacagg cagtggtcga acccgacagc cccttggctt cctacactgc
    20401 cgacttggaa ggtctgctgc tggtcaacgg caaacccgaa accgtcggca tcagccacgt
    20461 tgccgccctg tcgcctttga acagcgcgcg taaacaggaa cttgccgccc acgacggcgc
    20521 actcatccgc atcctccctt cggaaaacgg actcgacatc ctgcaagtgt ttgaagaaat
    20581 ctcttgcagc gtcaacacca cagccgccgg cggcaacgcc agcctgatgg cggtcgccga
    20641 ctgattttac cgaaatcccc gggcgcgggc cgtgaaccaa tgccgtctga aaacctttca
    20701 gacggca
//
LOCUS       DAWGJV010000019        20567 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWGJV010000019 DAWGJV010000000
VERSION     DAWGJV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.019"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            99..740
                     /locus_tag="WHQ94_000504"
     CDS             99..740
                     /locus_tag="WHQ94_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ2127834.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            1719..2519
                     /locus_tag="WHQ94_000505"
     CDS             1719..2519
                     /locus_tag="WHQ94_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ2127835.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(2971..3609)
                     /locus_tag="WHQ94_000506"
     CDS             complement(2971..3609)
                     /locus_tag="WHQ94_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ2127836.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            3864..4706
                     /locus_tag="WHQ94_000507"
     CDS             3864..4706
                     /locus_tag="WHQ94_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ2127837.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVERPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            4772..5434
                     /gene="exbB"
                     /locus_tag="WHQ94_000508"
     CDS             4772..5434
                     /gene="exbB"
                     /locus_tag="WHQ94_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ2127838.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5455..5889
                     /locus_tag="WHQ94_000509"
     CDS             5455..5889
                     /locus_tag="WHQ94_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ2127839.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6380..6808)
                     /locus_tag="WHQ94_000510"
     CDS             complement(6380..6808)
                     /locus_tag="WHQ94_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ2127840.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(6873..8621)
                     /locus_tag="WHQ94_000511"
     CDS             complement(6873..8621)
                     /locus_tag="WHQ94_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ2127841.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTENLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            8948..10381
                     /gene="ccoN"
                     /locus_tag="WHQ94_000512"
     CDS             8948..10381
                     /gene="ccoN"
                     /locus_tag="WHQ94_000512"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ2127842.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10408..11019
                     /gene="ccoO"
                     /locus_tag="WHQ94_000513"
     CDS             10408..11019
                     /gene="ccoO"
                     /locus_tag="WHQ94_000513"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ2127843.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11024..11194
                     /locus_tag="WHQ94_000514"
     CDS             11024..11194
                     /locus_tag="WHQ94_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ2127844.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            11218..12315
                     /gene="ccoP"
                     /locus_tag="WHQ94_000515"
     CDS             11218..12315
                     /gene="ccoP"
                     /locus_tag="WHQ94_000515"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ2127845.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKALVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            12428..12553
                     /locus_tag="WHQ94_000516"
     CDS             12428..12553
                     /locus_tag="WHQ94_000516"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ2127846.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            12755..14164
                     /locus_tag="WHQ94_000517"
     CDS             12755..14164
                     /locus_tag="WHQ94_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2127847.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            14266..17475
                     /gene="recC"
                     /locus_tag="WHQ94_000518"
     CDS             14266..17475
                     /gene="recC"
                     /locus_tag="WHQ94_000518"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ2127848.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGSRDTLLHALQTDIQNLDMPSEKAGNINTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWQQPATVGEWVARCRDLLETLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGQDFSQTAARIRAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="WHQ94_000519"
     CDS             complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="WHQ94_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ2127849.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            19445..19657
                     /locus_tag="WHQ94_000520"
     CDS             19445..19657
                     /locus_tag="WHQ94_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127850.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYENIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(19750..20382)
                     /gene="mtrR"
                     /locus_tag="WHQ94_000521"
     CDS             complement(19750..20382)
                     /gene="mtrR"
                     /locus_tag="WHQ94_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ2127851.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT         5283 a         4712 c         5361 g         5211 t
ORIGIN      
        1 ttatttattt acggtgcata ggcgggaaga taaacttgtt ccaggcggcg cggggtgtta
       61 aggtagccag taccgacagg ttgacgagga atattttgat gaaactgtat atttacgacc
      121 attgcccgtt ttgcgtgcgt gcgcggatgg ctgccgggct gtttggcgcg gacgttgaag
      181 aagccgtgct ggcaaacgac gacgaggcaa cgccgatcgg tatgatcggt gcgaaacaag
      241 tgcccgtcct gcaaaaagaa gacggttcgt ttatgggcga gagtttggac attgtgcggc
      301 atttcgaccg cgaagaccgt ctgaaagacg aagtccgccc cgagatacgg gcgtggctgg
      361 acaaggtggg cggatacaac gacaaactgg tgcagccgcg cgtgatcaaa atcgggctgc
      421 ctgaatttgc aacgccggaa gcggtgaaat attttacaga caaaaaagag aaaagcatcg
      481 gcagcttttc cgccaatttg aataaaaccg cccaatacct cgaacggata aacgcagatt
      541 tgcaagagtt ggaaaatttg atggacggtg cttcagacgg catcaacggc ggaatcggta
      601 tggaggatat tttggttttc cccgttttga ggaatctgac cgtggtgcgc ggcatcgaat
      661 ggccccaaaa ggtgatgggt tatttgatga cgatgagtga aaaatcgggc gtgccgcttt
      721 attttgacag ggcattgtaa aagtggctgt tattaactga cagatgaagg aggttctacc
      781 ctatttgagg taaactctat ttaccattgg ccgaaatacc agtgcttatc tccaagacga
      841 ccttcccaat cccttttgcc tcttaaaaag aagtgactag tgtgtaaagt tatagttcct
      901 tttatagctt tccaataaaa ttaaagtatt taaaagcttg tttagttcta atttgttaat
      961 ttctgtatct aaataatgtt cagaatagta ttatttttta aataaaccat gaatagtact
     1021 tcacattgtt tgtcgtatta tttagctttt aaatttctaa tgtgaaagat agtttaatgg
     1081 acgcctttga tttttttatg caaggcaatt ttaaaaaaat tgcaactgaa caaggtttag
     1141 accgtaaata ttttatattc cactatatcg tatgtaattt atctgccatt tcttcttttt
     1201 gtttaatgaa ggttatccat agttatacta tcctttgttt attaataatg ataattggca
     1261 ttgcatttgg atttatcttg agaaataggg ttctatctat agtaaaaaat aaacaaaatt
     1321 ttttatctga tatgtttccc ttggaaatgc ctttcttttc tagagtatac aaaataccta
     1381 tcttggccag attcttgaat aggggaatga aatggcttta tctccaaaat tttttctgtt
     1441 tagttatgat ttgtttctcg atatctctta tccttaatga agaaaggggg tggatgcctt
     1501 attttatctg ctattggttt ggactttgtt atccagcaaa cacttattac gtttttttat
     1561 ccagagatta taaacatatc taaactatta tgcgtggcgg tacttccaaa ggtaaatttt
     1621 aacctaatca atttactcaa accatccaaa aattaccaag cagcaatata aacacagatt
     1681 tatttgagcc aacctgatca aaacaaggaa ctaaatagat gaaaaagacc atttttaaca
     1741 ttaccaaaat ggactgcccg tccgaagaac agctcattcg catgcgttta aaagatgtgt
     1801 ctgatattta tgaattacag tttgatattg ctgggcggtg cttaaccgtc tatcacgata
     1861 atcaagatac aacgatttta caagtacttg aaccgcttaa ttttgatagt catatcattt
     1921 ccaccgaagt gattgttgat aagatagtgt ttaacaagcc tgatgagcac ttagaaaaaa
     1981 gattattata tcaagtgctc atgattaact ttgtgttttt tatcatagag tgttcggttg
     2041 gtatttttgc taattctatg gggctgattg ctgatagtct agatatgctg gcggacagct
     2101 ttgtctatat attggcgcta tcagccattg gtatgacgct ggcgtataaa aaacgtgtgg
     2161 catttttggc ggggataacg cagataatat tggcgttatt tggggtgata gaagtgatta
     2221 ggcgttttat cggtgctgag cagctgccca attatcaact gatgattggc acggcatttt
     2281 tggcattgat tgcaaactgg ctgtgcttat atctactgag caaaaatcaa aataaagaaa
     2341 tccatatcaa agccagcatg atttttacat ccaatgacat tatcatcaat atcggtgtga
     2401 tagcggcggg agcattgacg ttattgactc attctagcta tcctgattta atcattggca
     2461 tgattgtctt tgtcattgta ctatttggtg ctagaaatat tttaaaattg gcaaaataat
     2521 tcctttcacc agcaaaggag tttttatgca aatatcggtc aaagctacct acatacgtgg
     2581 cagtacttcc aaaaacttga ttttttaaaa tgtggttaat gcgagtttgt ttatctggta
     2641 tttgattggt caagccttca tccaagcgtt tgacgatttc acgcaaaaat acccctgatt
     2701 tacacacagg gtctaagaat gtcgcctttg gatttgacca aatctcagac ggcaataaat
     2761 ccagcatttg attggcaagt tttggcgatg tgaaaacctc atcattagac agactggagt
     2821 ttcttaatca gtgtttcagt taatgaaagg tattatcatg caacctggtt ctaataatga
     2881 tgaaaaaaat aagattacac tacaggaaaa cgcggcaggg caatatgtcc tgccgcgttt
     2941 tctggagcgt ttatgccgtc tgaaagccag tcagacgaac atctgcacaa acgcaatcac
     3001 cagccgcaca atcggtgcaa taaacgcacc caaaaccccg gtcagcatca gcagtaggat
     3061 aatccacgtc ccataaggtt cgattttgcg gaacgcttgc gaatatttcg ccgacaggaa
     3121 ggtgtcgatg aaaatgccgc cgtcccaagg caggatgggg atgatgttga gcgcgaacag
     3181 aatcgcattg atcagaatac cgtagtttgc catttgagcc aacggcatct gatacgcccc
     3241 gccgacatac ggagtcagca ccaaaaccac gccccacaga acagccatcg ccagattcga
     3301 cagcgggccg gacgcggcaa cgcaacgcca ggcaaggcgc gggttgcgga agttgcgcga
     3361 atcgatagga atcggacgcg cccagccgaa caggaagggc gtgaacatca aagtaagcag
     3421 cggtacgatg attgtgccga ccaaatcgat atggggcagg gggttcagtg tcagcctgcc
     3481 gtattgttcg gcagtgttgt ctccccagta gcgcgccgta tagccgcgcg ccacctccct
     3541 gacggtaatg gcgagcagca cgggcaggac ggcaagcaga aacacgccca aatcaaaatt
     3601 ttgaaacatg gttttccttt gaacagatgt ggtgtgcagc ccgaaacggc tgacagacag
     3661 aagcggcaat atatcatcta acctgatttt attggaataa gaaatcagac ggaatcattt
     3721 tttctgccgg cccgttatct gatgatttgc tgtaatacag cgtttaaaca cggtttgcct
     3781 gtgaacggaa cagacattca tatgaccaaa aattgtaaaa ataggttgca aataggaatt
     3841 gttgctatta ttatgcccga attatggata aagaacgaat tttaaccccc gcagtcgtgt
     3901 tttccgtcgc actgctgcat ttggcaatgg tggcattgct ctggcaggcg cacaagctgc
     3961 ccgtgataga gtcaggaaat gttatcgaat ttgtcgattt gggtgatttt ggcggagggg
     4021 acggtgcacc cgaaggcgca ggtgcgcctg ctgcgcctga gccgcaacct gagcctgagc
     4081 cgcccaaacc tgtcgagccg cccaagccgg ttttgaagcc ggtggttacg aaaaaggcgg
     4141 atgcggatat tcagcagcct aaggaagagc cgaaacctga agaaaagccg aaacccgaag
     4201 aaaaaccgaa accagagcct aagccggaag cgaagcctgt cccgaaaccg gcggaaaaac
     4261 cggtcgagag gccgtctgaa aaacctgccg aacatcccag caatgcttct gccaaagcag
     4321 acagcgagca gggtaatggg gaagataagg gaaccggtac caaaggagac ggaacggggc
     4381 gcggagaagg cagcggtaaa ggtagcggcg gtgtcaaagg cgaacacggg gaaggagccg
     4441 gcagcagcaa aggcaatcct ttacgcgcca acggcagcat tccgcgcccg gcttatccca
     4501 cgctttctat ggagaatgac gagcagggta cggttgtttt gagcgttttg gtttctccgg
     4561 gcggtcatgt cgagtccgtt aaaatcgtga aaagcagtgg tttttctcgg ttggacaatg
     4621 cggcacgcaa ggcggcgcaa aacgggcatt ttcaagccaa tgcctggacg gagtttaaag
     4681 tccccgtcaa gtttgaattg aattaggcgg ttgccgcagc ggtttgcggc agcttggatg
     4741 tttgattggt tttttcagtg ttggaatcat tatgaatttg aaattagtat ttgaatcggg
     4801 cgatcccgtc ctgatcggtg tgtttgtgtt gatgctgttg atgagtatcg taacgtggtg
     4861 tttggttgtc ttgcgctgca tcaagctgta tcgggcgcgc aaagggaatg ccgccgtcaa
     4921 acggcatatg cgcgatactt tgtcgctgaa cgacgcggtc gaaaaagtgc gcgccgtcga
     4981 tgcgcctttg tccaaactgg cgcaagaggc attgcagtct taccgcaact accgccgaaa
     5041 cgaagcgtcc gaactggcgc aggctttgcc gttgaacgag tatttggtca ttcaaatccg
     5101 caacagtatg gcgcagatta tgcgccggtt tgattacggg atgaccgcgc ttgcctccat
     5161 cggcgcgacc gcgccgttta tcgggctgtt cggcacggtt tgggggattt accacgccct
     5221 gatcaatatc gggcaaagcg ggcagatgag tattgcggcg gttgccggcc cgattggcga
     5281 ggcactggtg gcgacggcgg cgggtttgtt cgtggcgatt ccggcggtgt tggcatacaa
     5341 cttcctcaat cgcggcacaa aaatactgac ccaggatttg gatgcgatgg cgcacgattt
     5401 gcacgtccgc ctgcttaatc aaaaggatag ctgattataa aaggatagct gattatggca
     5461 tttggttcga tgaattccgg cgacgattct ccgatgtccg acatcaacgt tacgccgttg
     5521 gtggacgtga tgctggtgtt gctgattgtg tttatgatta ctatgccggt gctgacgcat
     5581 tccatccctt tggaactgcc gaccgcgtcc gagcagacaa acaagcagga caaacagcct
     5641 aaagaccccc tgcgcctgac gattgatgcg aacggcggct attatgtcgg cggggattct
     5701 gcaagcaaag tggaaatcgg ggaagtggaa agccgtctga aagccgccaa ggagcagaat
     5761 gaaaacgtga ttgtggcgat tgcggcagac aaggcggtgg aatacgatta tgtaaacaaa
     5821 gctttagaag ccgcccgtca ggcaggaatc accaaaatcg gttttgtaac cgaaaccaag
     5881 gcgcaataaa gttcgacgct tgaacgaatg ccgtctgaaa gtctttcaga cggcatttct
     5941 tatgtttaat cagctatata tagttgatta aaacaaaaat gagacgacag agcgccgtct
     6001 ccatttctgc gcaggcggga atacaaacat ctgatttttc ttttattttc aaacattact
     6061 gaaatgggta agtctggagt cctgcctgcg cgagaataat gaaaatgtgc atttctaatt
     6121 tttatagtgg attaacaaaa atcaggacaa ggcaacgaag ccgtagacag tacaaatagt
     6181 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagttaaggc
     6241 gaggcaacgc cgtactggtt tttgttaatc cgctataccc actgtaaaaa cggagaagga
     6301 cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt tgcggtggaa tgccgcttga
     6361 aggtgttttc ggcttgtaat cagacgcggg cgttgtccag ccgctctctg aaattttcca
     6421 aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc aaccggcata ccttctgccg
     6481 cccggcggaa gccccacagg agggagcagc gcgtaccggt ccggcaatag gcaaggacgg
     6541 gatattcggc ttgtccgatg agttggcgga aggtttcgac atcgtgtttt tggatgtcgc
     6601 gtgcggtaac gggttggtga tggaatccag taacgcctgc ttgttccagc cactgtttga
     6661 tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg gcggttgcag atgacggttt
     6721 tgatgcccag ttgcgcgatt tgttccgcat cggctttggt cagttgcgga gaaatataga
     6781 ggtgttcgtc aagtttcaga atggccattt tgtttccttt actttgcggt tgtttttcag
     6841 acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga acaaccagtt gtccaacagg
     6901 ttccggtctg tgatatgtag gatggcagta tccgctgccg ctgcctgaac cgtcaagacg
     6961 gtttgacgca atatgccggc acggaagaag tggatattga tttttgcatt gacgtggtat
     7021 cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt ctaaagcaat gattttgtct
     7081 tgcggacaca gtgccgcaga ttccgcgctg ccgccgttga agacatgggt caggacgatg
     7141 tggtcggcgt tttgtttgaa acgtgcgcca aaatcgcctg ccgacgggac ggggcagatg
     7201 tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca ggaaggtcag tcccacgcct
     7261 gcggttgcca ggcattcggc aagcggcaaa ttttcggtac tgtataacgc tttttggaaa
     7321 aaatcttcca aatccaagcc ggtaatttcc tgacagcgga tttgccagtg tttttccgga
     7381 atacccgagt gtgtgtccct ccactcccga tagagtttgt ccattatcgt atcgagagaa
     7441 tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc acaatgcggc aagcgcgcct
     7501 ttctggtagt agctgacgat ggcgttgggg ctgttttcat caggtttgta aaatttgttc
     7561 cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac ggccgcgggt ttgttgtacg
     7621 cgcgtaatgc cttgtgccag caggtttaaa taagattcgg gcgagatggt gcggctgcgt
     7681 gccaaaaaca aatcgtcgta ataggatgta atgccttcga atgcccatag ttgttcggta
     7741 tagttttctt tgtcgaggtc ataaggggcg aacgcggcag gtttgatgga tttgacgttc
     7801 cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg tggtgtaggc atcgtcggca
     7861 tcggtcatac cgaacggcgg aaggctgtgg cggtcggcga gcagggcggt gctgtcggcg
     7921 tgttccaaac cgccgtaaat atggtcgccg acgtggagca ggaacaaata tttttgaaac
     7981 ggggcagggg aggaaaacac cgccagttct gtttcgcaga tttttttgat atccgaaacc
     8041 agcctgttgc ggtcgaaatc gggatagatg ccgcttaagg caattgtgtg cggaatgcct
     8101 gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga caggtcggtc aatcaattcg
     8161 gcataagatg ccgcctgaaa gacaaacctc cctgtttccg gcagcgttgt ggcaatacgc
     8221 cattcggacg gaatacccgt caattccaag cggtgcggca gcgtttccgt tccttcgact
     8281 ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg tcgtcaggaa agaacctcga
     8341 accgacaaat cgaatgcata tacggtgtag cggatttgcc actcgccgcg tacggcggca
     8401 gcgtgccagc ggttttttgc aatttgttcg agcggcatgg acgtgccgtt acaggatgca
     8461 tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat agctgcccgg aacccagttt
     8521 ggcaggctta tttctatagg aagatcatca tcttgtgtga atgtcagcag gatgtgccac
     8581 tcgtggtcga gaggggacgg ggttatttta taatgaatca taactatcaa atatatcgtt
     8641 aaacgggatg gattaaatac ctttaaaatg cagcttaacc tacatcaata aagtgccaca
     8701 aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt gtaatttatt ataacaatat
     8761 gatttatatg taataaaaaa cagattaagc aaagggctcg ccggcggcgc gaatgtaatt
     8821 ccgcttggcg taataaggca gatatgttaa atttagaaaa aattacgctt gcccggattg
     8881 caccttaaac aggctgcgga agcggcagga taaaaatctt tattcccata aattcatgga
     8941 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     9001 tgtagtttgg gggattgtgg gtatgttggt cggcgttatc gtcgccgccc agctttttgc
     9061 tcctgccctc gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     9121 gcacaccaat gcggttattt tcgcatttgg cggttgcggc ctgatcggca catcatacta
     9181 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     9241 ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     9301 aggtaaggaa tatgccgaac tggaatggcc gatcgatatt ctgattactt tggtttgggt
     9361 ggcttacgcc atcgtattct tcggtacgat tgccaaacgt aagattaaac atatttacgt
     9421 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttacaca tcgtcaacaa
     9481 tatcagcatc ccggcagggt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     9541 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     9601 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     9661 gtccgtcgtt cacttctggg cgttgatttt tacctatatg tgggcgggtc cgcaccatct
     9721 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ctttgattct
     9781 gttcgcaccc tcttggggcg gtatgattaa cggcatcatg accttgtccg gcgcgtggga
     9841 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
     9901 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
     9961 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
    10021 cggttccgtc tattacatga ttccccgtct gttcggcaaa gaacagatgc acagcaccaa
    10081 gctggtagaa gcacattttt ggattgcgac catcggcgtg gttctgtata tcgctgccat
    10141 gtggattgcc ggtgtgatgc agggtttgat gtggagttct ttgaacgatg acggtacgct
    10201 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacgtga tccgttttgc
    10261 aggtggttta ttgtatctga gtggtatgtg cattatggcg tacaacgtgt accgcacagc
    10321 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
    10381 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
    10441 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
    10501 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
    10561 ttgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactctcaa
    10621 atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc attactctgt tgccggagag
    10681 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
    10741 gtgggcggtc gctattccga cgaatggcac cgtatccacc tgctgaatcc ccgtgatgtc
    10801 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
    10861 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
    10921 attgcgaaag cacctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
    10981 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
    11041 tcgtgctctc ttcacggtat ggatctttat ctgtttcctg ttggtactct atatcgtctt
    11101 caacaggcgg aataagaaaa actacgataa tgccgcaaac agcatttttg atgaaaacca
    11161 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
    11221 aacacaacat cccaatttac tagtaatttc tggaatatat atattgcagt tattgtctta
    11281 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
    11341 aagggcgaag aagtacaaac tacgggtcat gagtgggacg gcattgccga atacgacaat
    11401 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
    11461 ttggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
    11521 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
    11581 gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag ccaagcaaat cgcccaaaac
    11641 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ctaaaggctc taaaggtttt
    11701 ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc ccgataaaat tcacgagacc
    11761 atcgaaaaag gccgtgttgc gactatgcct gcctggggcc ctgctttggg cgaagaaggc
    11821 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccgaaggtca gtatgatgag
    11881 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
    11941 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
    12001 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggtcg cagcagccaa
    12061 atgcccgctt ggggacattt cttggataaa gacaaactgc atattatgac tgcttatgta
    12121 tggggtcttt ccgataaaga cggtaaagct ctggtgaaaa aagccgagcc tgcaccgact
    12181 cccgcaccgg cggcagaacc cgcagcctct gctcctgcag aagcagcaca agccgcgtcc
    12241 gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa aagccgcacc tgctgccaaa
    12301 gcggacggca aataggttta tgaaaccgtt tgtgccgcct gccatggcaa tgcggttccg
    12361 ggtattcccc atgtaggcat caaagccgat tgggccgacc gcatcaaaaa aggcaaggac
    12421 acgttcgttg cacaaacacg cgatcgaagg tttcaatacg atgcccgcaa aaggcggtcg
    12481 cggcgatttg agcgatgatg aagtgaaagc tgcggttgac tatatggtca accagtcagg
    12541 cggcaaattc taacttgact gagtttccga tattatcggt tatgaaaatc cagattcagt
    12601 attgaatctg gattttttgt tttaacgggc atccggtttt aaacgcagac tgcctatttg
    12661 ttgtaatgaa ttttgacttt agtcataaag aattagtctt tattgtttat aatcgcgcat
    12721 catcacgata gccctatttt caaaaggacg attaatggat acacaaatca aaactgaagc
    12781 cgacaatcag agcaaccggc gttatctgac cgtttggcgg tggcattttt atgccggtct
    12841 gctggttatg ccttttctta ccctgcttgc cgttacgggt ttgggtatgc tgctgtttgc
    12901 caatattacc ggtaaggagg gcgagcggat tcatgttgtg ccgcaggcaa cggtacaacc
    12961 tctgtctgtt caggcggaag cggcacgcag tgccgttaat ccggaaactt cgtccgtcgt
    13021 tcagtatatt gcgccgcgtg ccgatgatat ggttgccgtg ttccgtgtca acaatgaggg
    13081 caaggcaacg atggtcgcgg tcgatcctta tacggcaaaa gtggtcagta ccatgccgcg
    13141 caatcagggt tggtattacg cgatggatga aatccacagc gatatgatgc tcggttcgac
    13201 aggtgattat cttttggaaa cggctgcatc gctgacgatt atcatgataa tcagcggttt
    13261 gtacctttgg tgggtgaaac ggcgcggcat caaggcgatg ctgctgccgc caaaaggcag
    13321 ggcgcgttct tggtggcgga atctgcacgg cgcgtttgga acttgggtgt cgttgatttt
    13381 actgttgttc tgcctgtcgg gtattgcttg ggcaggtatt tggggcggca agttcgtgca
    13441 ggcttggagt cagttcccgg ccggcaaatg gggtgtcgaa ccgaaccctg tttcagtcgt
    13501 gccgacccac ggcgaggtat tgaatgacgg caaggttaag gaagtgccgt gggttttgga
    13561 gcttacgcct atgcctgttt cagggacgac tgtgggcaaa gacggtatta accctgacga
    13621 gccgatgaca ttggaaaccg tcgaccgttt tgcgcgggaa atcggtttca aagggcgtta
    13681 tcagttgaat ttgcccaaag gcgaggacgg agtgtggact ttgtcgcagg attctatgag
    13741 ttacgacatg atcagcccgt ttgccgaccg cacggtgcat atcgaccagt acagcggcaa
    13801 aatccttgcc gacatccgct ttgacgatta caacccgttc ggcaaattta tggcggcaag
    13861 cattgcgctg catatgggta ctttgggctg gtggagcgtg ttggcgaacg ttttgttctg
    13921 ccttgccgtc atttttatcg gtatcagcgg ctgcgtgatg tggtggaaac gccgtccgac
    13981 cggagcggtg ggcatcgttc cgccggcgca gaaagtcaag ctgccggttt ggtggatgat
    14041 ggcattgccg ctattggcaa tcgcactgct cttcccgacc gcactgcttg ccattgccgt
    14101 gatttggctg ttggatacct tgctgctgtc gcggattcct gttttgagga gatggtttaa
    14161 atgacccgat gccgtctgaa gcgttcagac ggcattttgt ttgaaggcgg gcgggggaaa
    14221 ggctatataa tcccgaatac ttgaccacag cttctgtttg aaatcatgtt ttatctgtat
    14281 caatccaacc gtcttgaaac gctggcggca ctgtttgccc gcattcaaaa agtcaaaccg
    14341 ctgaaatcgg ttttacagcc cgaacagatt attgtgcaga gtcaggggat gcgccgctac
    14401 ctcaatacct gcctcgcccg cgatttgggc gtggcggcga atttgtcgtt cagcctgccc
    14461 gccggtctga cgtggaagct gatgaaaaaa ctgattcccg gtattccgga actcagcccg
    14521 tttgcgcctg aagtgatgcg ctggcggctg ttggatttgt tccgcagcga ggcatttcgg
    14581 aatacggcag aatttgaaga tgtgaggaat gtgctgcaag actatctggg cagcggcgaa
    14641 tcggcggatt atcagcttgc tggccagctt gcagacatat ttgaccaata cctcgtgtat
    14701 cgtccgcagt ggatagacgc gtggcagcag ggcaggatac tcagtttggg cgacgatgaa
    14761 atctggcagt ccaaactgtg gcgttatctt gatgacggca ggcagagcgc gccgcaccgt
    14821 gttgcgttgt gggaaaagct gttggaatct ttgagcagtg ataagctgcc cgagcgttat
    14881 ttcgtgttcg gcatttccac gatggcgccg atgtatttgc agcttttgca caagctgtcc
    14941 gaacattgcg atgtgttcgt gtttgcactc aatccgagtg ggatgtattg gggcaacgtc
    15001 atcgaagcgg cgcaaatcct caaaggcggc ggcgatcccg atttaactca ggcagggcat
    15061 ccgctgctcg cctcattggg caagcagggg cgcgactttt tcgacttttt gaacgaaatg
    15121 gaaatagaag gagaaacgcc ggtgtttgaa gaaggcagcc gcgatacgct tttgcacgcc
    15181 ctgcaaaccg atatccaaaa cttggatatg ccgtctgaaa aagcgggaaa tattaatacg
    15241 gacgacggat cgatacgcat cgtctccgca cacagccctt tgcgcgaatt gcagatactc
    15301 aaagacaaac tgttgaaaat tctgcatgaa catcccgatt ggcagccgca tgatatagcc
    15361 gtattaaccc cgaacatcga atcctatacg ccttttatcg aagccgtgtt cggacaggcg
    15421 cagcccggtg cgcaggcatt gccgtattcc gtttcagacg tgaaaatcag ccgccgacaa
    15481 ccgttttttt atacattgga atgcctgtcc gacttgttgg aaagccgatt tgaagtcgat
    15541 aaagtgcttg tgcttttaga aaccgcaccc gtgttgcgcc gtttcggact gactgaggac
    15601 gatttgccgc ttttgcacga catggttgcc gatttgaacg tccgctgggg tttggacgga
    15661 gaaatgcgcg gcggcacgga tcagctgttt acctggaagc aggcggtaga acgcatgata
    15721 ttgggctgga tgctgcccga aggcggcaat ccgatgtggc aggatgtcag cgcgtggtat
    15781 gccgacgtga accaaaccgc cctgttcgga cgttttgccg ccctcctcga aaccctgtcg
    15841 gatattgtac ggatatggca gcaacctgcg acggtcggcg aatgggtggc gcgttgccgg
    15901 gatttgcttg aaacattgtt ccaaaccgaa gccgatgacc aaaaggcagt ccaaaacctc
    15961 gaaaacgaat gggtcaaatg gcaggcggaa accgaattgg cgcaattttc cggacagttg
    16021 ccgccgcaca ccgtcatccg ccacatccgc cgctttctcg acagcgaaag cgaggcaggc
    16081 tttttacgcg gcggcatcac cttttgcagt atggtgccga tgcggagcct gccctttaaa
    16141 gtcatctgtc tgttgggttt gaacgacgga gattttcccc gcaataccaa agccgccgta
    16201 ttcgacctga ttgccaaaca tcccgccaaa ggcgaccgcg cccgccgcga tgacgaccgc
    16261 tacctgttcc tcgaagccct catcagcgcg cgtgaaatcc tctacctgtc ctacatcggg
    16321 cgcgacatcc gcaaagacga agagcttgcc ccgtcttccc tgttgggcga actcatcgat
    16381 accgttgccg ctatggcggg catcggtagt cgccaacttg cacaaaactg gatagaacag
    16441 catccgctgc aagccttttc acgccgatat ttccaagaag gcggacgttc agacggcata
    16501 ttcggcacgc gtaccgacta cgccaccgcg ctcggacaaa cgcccgaacc gccgcaaccc
    16561 tttttcgacc aacccgtaga aaacgccgaa cctgttgccg aaatcggaca ggacgaattt
    16621 atccgtttct ggcgcaaccc cgtcaaagta tggcttcagc agcagcttgc gtggagcgaa
    16681 ccccatatcg gcgaagcctg ggagcctgcc gaacccttcg agccgcaaca cgccgatcaa
    16741 atcgccgaaa tctatattga ggcgcgccgc gaaggacaag atttttccca aaccgccgcc
    16801 cgcatcaggg cggaaagcct gctgccgtcg ggagagttgg gcagactttg gcagcaggac
    16861 ttccaaaccg ctgccaaaca aatcgacacg gcggttttaa acagtcccaa acttcctccg
    16921 ctgccctacg ccataccgtc ggacgggcaa atcctgaaag gcagcttggg caatctgtac
    16981 cgctgcggac aagtgtttta cgcctacggc aaacccaacg cgccgcaacg tatcgctttt
    17041 ttgttggaac acctgatatt ttgcgccgtt atgccgtctg aagccgaaat gcggcaaacc
    17101 tttatcgtcc aatccggaga aaccgaaata ttggcggaaa tcgcgcaaga cagggcattg
    17161 cagctattgt cggaatggat ggcgtttttc aatatcgggc aaaaccgccc gctgccgttt
    17221 tttgccaaaa cctcgcttgc cgccgccgaa gcgtttgccc aaaaacaaga ttgggaagcc
    17281 gccctgaaaa aagcccaaac cgcctattac ggcagcaaag tcagcaaagg gcagaaagac
    17341 tataccgaag tcgccctcgt gttcggcaac gcaagccaaa acccgctcga acagcccctg
    17401 tttgaaaacc tcgtccgtct gcttgccgac acgtttgccg cagcggaaaa aaaggaagag
    17461 gccggagcag cctgacggga tggaaaggca aatgccgtct gaaaacagca aaaaggtttt
    17521 cagacggcat tttgtttatc gtgtttaagg tgcgggatag aatgtgggcg cgccgggacc
    17581 gacgggcagg cccaaaacaa atacccaaat gcagaataag gcaatccacg caatcaagaa
    17641 gaaagcggaa tacggcaaca tcatagaaat cagcgtaccc acgcccgcat cttttttgta
    17701 tttgatcacc gtcgccataa tcagcccgaa ataactcatc atcggcgtaa taatattggt
    17761 aacggaatca ccgatgcggt aagcggcttg aatgacttcg ggcgcgtagc cggccaacat
    17821 cagcataggg acgaaaatcg gcgcagttac cgcccattgc gcggaggcgg agcctatcat
    17881 cagattgata aaagcacaaa ttaaaataaa accgataaac aacacgctgc cgcccaagcc
    17941 gacttctttt aagaacgtcg cccctttaac ggcaatatat tgcccaatat tcgtccaatt
    18001 aaaaaatgcg acaaactgtg cggcaaaaaa gatgatgacc aaataaagcc ccagagtact
    18061 catcgattcg gccatcgcat taacgacttc ctgttcgccg cgcaaacttc gggttaccct
    18121 gccataaaca atgcccggca gcgcaaacaa caagaaaata aaaacaacaa tcgattttaa
    18181 aaacggcgaa ccggaaacca atcctgtttc aggatgacgc aaaataccgt cggcagggac
    18241 gatgctccaa gccaataggg cggataaggc aacaaacacc acgccagccc aaattaatcc
    18301 tttatattcc aaaggcgtga tttcattgga atgccgaatg tctttttctt cttgtgacaa
    18361 atctgattga taagggccca attgcggttc gacgattttt tcagtaacaa aataaccaat
    18421 caaagcaatc acaaacgtac tggctaccat aaaaaaccag ttggcttcag ggcctacgac
    18481 gtagtcggga tggatgattt gcgccgcctg ttgggtgatg cctgccaaga gcggatcgat
    18541 tgtgcctaag aacagattgg ccgaataacc gcccgaaacg ccggcgaaag ccgcagccag
    18601 accggcaagc ggatggcggc cgagggaatg aaagatgatg gcggacaaag ggattaggac
    18661 gacatagccc aattcagaag cggtattaga taaaatccct gtaaaaacaa ccataaaagt
    18721 agtgagtttg cgtggagatt ttgtgagcaa taagcgcatt aatgcggaaa tcaagcccga
    18781 tttttccgca atccccacgc ccaataaaga aaccaacacc gttcccaacg gcgcgaaacc
    18841 ggtgaaattt ttaacggtat gcgtcaggat tttgatcaaa ccgtcagcat cgagcaggct
    18901 gacaacgtga atcaaaccgt catcggcacg tcctttcgca ccaacagggc gcggatcggg
    18961 gacggatagt ccgaaatacg caccggcggc agaggcaatc agcaataaca caatgaaaat
    19021 aataaaaagc gtaaccgggt gcggcaacat attgcccagc cattcgactg tgcgtaaaaa
    19081 tcgtccgtcc cgttgcgtat cggtttgact catctgcttc tccttaaaaa atatattgag
    19141 tgcattaaat ttttgaatgt aacatgaagt gttttgtaag caaactaaaa taatcgcata
    19201 tgtatataac cgcaatagat taaaacagaa aatgcccaca tcgttattct cataaaggcg
    19261 gtaatccgaa aacttgacgt taaagcctta tcaggaatga ctggaactca acaaagcggt
    19321 ttcccgcttt cgcggaaatg gcgggatttt cggtttttgt ttgcaaaaat gccggagaaa
    19381 aatgcagttt acattttgaa tggtattgtt attaattgta aataaatatt ggaaggtaaa
    19441 tgatatgaaa aatgaaattc aaaaaattat ggacaaatat gatccatggc atgaagatga
    19501 ttttgagtcg tatgaaaaca ttgccagaga tgtatcgttg atgacagata aaacgttcat
    19561 tgaacattat ttgttagaag tttattcaga agaaaacgga cattttgacc aagagaatgt
    19621 ccatgcaatg atagaagaaa ttaaaaatgc aatttagtga atatgtattt ttagaagaag
    19681 gctttgtgtc attttgatgc cgtctgaaag ggtttgttcg tttcagacgg cattgttacc
    19741 aaggcttgat tatttccggc gcaggtcggg atggttttcc aagttgtcca tcattatccc
    19801 gatgatgcgc ggggcggttt tgcccaaatc gaaacgttcg caggaagaga accaccgcca
    19861 aatcagcccg tccaaggttg atttgatgaa gatgaccgcc gtttccttgt ccaaatcgtc
    19921 agccaaatcc tgattttcca ccgcttcggt caaaacggcg gtaattttct cgcgccagat
    19981 tgcctgatgc ttgcgggcaa tggcgataac ggcggcgttt tgctccgtgt gttcgcattt
    20041 taaaaacagg atgttgtgga atttgtagta gatgtcgttg ctttgcagcc gctcgaaaaa
    20101 gtgcagcagc gtgtggcgga ataccgccca agaccctcct tcggcatctt cggcatcttg
    20161 cgcgatgcag ttttcgatgt cgtcgcagat acgttggaac agcgcgtcga acaagtcttc
    20221 cttatttttg aaatgccaat agagcgcgcc gcgcgttacg ccggcggctt gggcgatttc
    20281 gttgagcgag gtgcgcgcaa tccctttgcg gtaaaaggtt tccaaggcgg caagcatcag
    20341 gtgttctttg gttttcaagg cttcggtttt ggtttttctc ataatggcgt tttcgtttcg
    20401 ggtcggtttg atgagggcgg attataaaaa agactttgta accatgcaat cgtgtatgta
    20461 taatgaaacc catgaaattg agactacatc tcaactttga aaacaggcat aacctgcttt
    20521 gcaacccgtt cgaacattcg ggtatcaaag caggcggttt ttatatc
//
LOCUS       DAWGJV010000020        20153 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWGJV010000020 DAWGJV010000000
VERSION     DAWGJV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.020"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="WHQ94_000522"
     CDS             complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="WHQ94_000522"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ2127852.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1327..2514)
                     /locus_tag="WHQ94_000523"
     CDS             complement(1327..2514)
                     /locus_tag="WHQ94_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEZ2127853.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRSNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="WHQ94_000524"
     CDS             complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="WHQ94_000524"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2127854.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="WHQ94_000525"
     CDS             complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="WHQ94_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ2127855.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPNKFEGSKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQADIQDDGCAGGACKI"
     gene            6386..6697
                     /locus_tag="WHQ94_000526"
     CDS             6386..6697
                     /locus_tag="WHQ94_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ2127856.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            complement(6971..7738)
                     /locus_tag="WHQ94_000527"
     CDS             complement(6971..7738)
                     /locus_tag="WHQ94_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ2127857.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(7826..8653)
                     /gene="mutM"
                     /locus_tag="WHQ94_000528"
     CDS             complement(7826..8653)
                     /gene="mutM"
                     /locus_tag="WHQ94_000528"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ2127858.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAL
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(8705..9370)
                     /locus_tag="WHQ94_000529"
     CDS             complement(8705..9370)
                     /locus_tag="WHQ94_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ2127859.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            9549..11525
                     /locus_tag="WHQ94_000530"
     CDS             9549..11525
                     /locus_tag="WHQ94_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ2127860.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASRSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRTRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFT
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            11593..12285
                     /locus_tag="WHQ94_000531"
     CDS             11593..12285
                     /locus_tag="WHQ94_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ2127861.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSIFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            12814..13257
                     /locus_tag="WHQ94_000532"
     CDS             12814..13257
                     /locus_tag="WHQ94_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2127862.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            13317..14126
                     /locus_tag="WHQ94_000533"
     CDS             13317..14126
                     /locus_tag="WHQ94_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2127863.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVTFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSVFGKNIFDAFDYISGNILFMLTAL
                     GSALFAGFVMKDEAKDELLYKGNHTTVNIWFAYVKYLVPLVILLIFVSNLF"
     gene            14370..15026
                     /locus_tag="WHQ94_000534"
     CDS             14370..15026
                     /locus_tag="WHQ94_000534"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ2127864.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            15181..16866
                     /gene="rpsA"
                     /locus_tag="WHQ94_000535"
     CDS             15181..16866
                     /gene="rpsA"
                     /locus_tag="WHQ94_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ2127865.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            16877..17191
                     /locus_tag="WHQ94_000536"
     CDS             16877..17191
                     /locus_tag="WHQ94_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ2127866.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(17542..17949)
                     /locus_tag="WHQ94_000537"
     CDS             complement(17542..17949)
                     /locus_tag="WHQ94_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ2127867.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            18097..19206
                     /locus_tag="WHQ94_000538"
     CDS             18097..19206
                     /locus_tag="WHQ94_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ2127868.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVLSVKPGDHVIPLYTAECGECEFCLSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVLQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IQLEPFVTHAMPLDQINEAFELMHEGKSIRTVIHY"
     gene            19215..20042
                     /gene="fghA"
                     /locus_tag="WHQ94_000539"
     CDS             19215..20042
                     /gene="fghA"
                     /locus_tag="WHQ94_000539"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ2127869.1"
                     /translation="MKLIEQHQIFGGLQQVWAHDAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQSINVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         5367 a         5160 c         4491 g         5135 t
ORIGIN      
        1 gatttttgtt aatccactat aatccactat aaaaggaaca ataaccgatc ctacccgctg
       61 tttttcccat catacaacat acaaatgccg tctgaaacat ccggcttcag acggcatttt
      121 ttcaaaaaac atttacaact caaaatcgcc caaatcatcc gtattcactt cagaatcgat
      181 ctgaccgatc aaataagagg atatttccac ttcctgcggc gcgacctgta cgttgtcgga
      241 cgacagccac gcattaatcc acggaatcgg gttttgattt gcgccttcaa atccggccgg
      301 caaccccacc gcctgcatac gcagattggt aatatattcg acgtattggg ataagatttc
      361 tttgttcaaa ccaatcatcg aaccgtcttt aaacaaatat gccgcccatt ctttttcctg
      421 ttccgccgct tttttgaaga gttggaaaca ttcgtcctgc aactcggcag caatttctgc
      481 catttcagaa tcatcaacac ccgaacgcat cagattaagc atatgctgcg tgccggtcag
      541 gtgcagggct tcgtcgcggg caatcagttt gatgattttg gcgttgcctt ccatcaactc
      601 gcgctcggca aaagcaaacg agcaggcgaa tgaaacgtag aaacggatgg cttccaacac
      661 gttgacgcac atcaggcaga gatagagttt tttcttcaac ccgcgcaaag acacagtaat
      721 gggttttccg ccgacattat gcatcccttc gcccaacagg ttgtaatact gggtgtattc
      781 gattaagtca tcgtaatagc aggcaatgtc ttcggcgcgg gcggtaatgt attcgttttc
      841 gacaatatca tcaaacacga ccgacggatc attcacaata ttgcggatga tgtgggtata
      901 gctgcgcgag tgtatggttt cgctgaagct ccacgtttca atccacgttt ccaactcggg
      961 aatcgaaacc aaaggcagca aggcaacatt cggactgcgc ccttggatgg aatcgagcag
     1021 tgtttgatat ttcagattgc tgatgaaaat atgtttttcg tgttcaggca ggttggcgta
     1081 gtcgatacgg tcgcgcgaca cgtcgatttc ttccggccgc caaaaaaacg acaattgttt
     1141 ttcaatcagt ttttcaaata cctcgtattt ctgctggtca taacgggcaa cattaaccgg
     1201 ctgaccaaaa aacatcggct cattcagcgc gtcgtttttg gttttgggaa aggtgctgta
     1261 tgacatagtt aagtgttcgc aggacataat ctattctcta tattaattaa aaatgatctg
     1321 taagttttaa ctaacctgct gagactttat atatttaata aaattttcta aagttaaaac
     1381 atgggttttc ccaaaagttc caaatgtttt tacggattct ttagggacaa ctaaggttaa
     1441 attctcatca ctcatttcct gaatttgagt atcagatata ccttcttgta gagtaaataa
     1501 atgcttatga ggaattcgat ctgcttcatt taaaacttgg cgccagcgat ccttacaagt
     1561 agtctttgaa cctagcattg tcagcttttc agtttgaaat atgaaattcc ctgattcatc
     1621 catcgcatgg tactcttcac ttccaggaaa taaaaaatct ggtttctttt tcccttctgt
     1681 cttcgcttgc gtctcaaatt ttaaggaaaa ttcgtcaaat attcttgata aatgtaactc
     1741 taaagatttt cctgctcttg cttttctgcg atttgtaaaa gaatgtgcaa aattaatgaa
     1801 gctatccaaa tcagtaattt ttgatttaat aatttcaaat tcacgttttt caaaaatgga
     1861 aaataattca tattcctttg aaacccattg ttttaacaca ttactaacgt tagttttatt
     1921 taatgcaata ttttcccttg ccaaaactgc catttcttca gttttgggga agttaggaaa
     1981 tagtgaaaat aattttgtta ggttatcctc tacttcttgt tttttaggta aatataaact
     2041 tcctggtaaa atgttagttt ctgcgataaa tatttcaata tcttcatctg aagataaaac
     2101 aaaagcatgg aataataaat ctttataaca agctctgcac aataccaata aggaaccact
     2161 atatttatca cttaaaaatt caaaattctt gccaaaaccc gtaattctcg cctcatttct
     2221 cgtgccttgg ccgtaatatt taaaattgct attagtgacg cttccatctt gccaattaat
     2281 tttgattgaa atagttttat ttgttccctt ttggcaaact acatcaaaga ggtcgtctga
     2341 aaaatgtttt tgaatataaa atcctgattg gtgactacca gtagtaccaa catcattgga
     2401 acgaatataa cgacagtata ctgcaattga tttatttgct gtctgtattg ctgaagctac
     2461 taagtcatcg gatttattta ctacttgtat tgtctgagct gccaagtcat tcatttttta
     2521 actcatttat aatttgttta gcaatagctt gaaccaacgg tacagcaatt gaattgccaa
     2581 actgcttgta tgcagctgtc ttggatactg catcaataac aaaatcttta ggaaatccca
     2641 ttaaacgcga gcactcccta ggtgtcagct tcctaggatt ttttcctttc tgagggatga
     2701 gtatttcgga accatctttg taatatcgtg cagatagagt tcgtgatatt ccatctaaat
     2761 caactaatcc aaaaccaaat ccattaccct ttgccttatg ttttttagcg taattttgaa
     2821 ggtaaagcca taagttatca gaaagagtaa aagaattatc tacatcatct tccaaaattt
     2881 gctttaattt tggttgtgat tctggtgggg aaggaaaatt gaaatttatt tccttattaa
     2941 aatattgtct atcaaaacct acaataaaaa tacgctccct attttgagga acataatatt
     3001 ttgcattcat aacttgataa aatatctgat agtcaagctc ttctaaagtc cctttaatta
     3061 ctttaaatgt atttcctttg tcatgcgaaa caaggttttt cacattctct aaaagaaaaa
     3121 ttttaggtcg atgttttcca ataatttcag caacatcaaa aaatagagtt ccctgcgcct
     3181 tatctaagaa gcctgtttct cgtcctaggc tttttttctt tgaaacacca gctatagaga
     3241 atggctgaca cgggaatcct gctgttaata catcaaactt acttggaata gctgctttgg
     3301 tttcctttaa tgtaatatct ccataaggaa tatcattaaa atttacttgg taggtttgac
     3361 gggctttatc atcccattca ctagaaaata cacatcgccc accaacattc tccattgcaa
     3421 tgcgaaaacc acctattccc gcaaataaat caataaaagt aaatttctca ttatttaagg
     3481 taactatatt atctaattga ttttgtattt tatttttcat attttatatt tcagatgatt
     3541 tattaatcta aaggccatct gaaaactccc cctttcatca aatcttacaa gccccgcccg
     3601 cgcagccgtc atcctgaata tcggcttgcg tatcgtccgc gccgtcgcgg gtgttgtggt
     3661 agtacagggt tttgacgccg tatttgtaag cggtcaataa gtctttgagc atttgtttca
     3721 tggaaacttt gctgccttcg aatttgttcg ggtcgtaggc ggtattggcg gaaatcgatt
     3781 gatcgacgaa tttttgcatc acgccgacaa gtttcaggta gccttcgttg ccgggaagct
     3841 gccacagggt ttcataggca tttttcaggg tttcaaactc cggcacgact tgtttcaaaa
     3901 tgccgtcttt cgatgctttg accgttacca atccgcgcgg cggctcgata ccgttggtgg
     3961 cgttggcgat ttgcgaactg gtttcagacg gcatgagtgc ggttagagta gagttacgca
     4021 ggccgtattt gacgatttcg gcacgcaggc tttcccagtc gtaatgcaaa ggctcgccgc
     4081 agacggcatc caaatctttt ttgtaggtgt cgatgggcag tttgccttgc gaataaacgg
     4141 tttggttaaa gagcgtacac gcaccgtatt ctttggcaag gtttgccgat gctttgagca
     4201 ggtaatactg tatggcttca aaggtacggt gggtcagacc gagcgcggag ccgtcgctgt
     4261 agcggacacc gtttttcgcc agataataag catagttaat cacgccgatg ccgagcgaac
     4321 ggcggcccat agtagaggta cgcgcggctt ctaccggata tccctgataa tctaaaagtg
     4381 catcgagcgc acgaacggtc aagtcggcaa gcccttccaa ttcgtccaag ctgtttaatg
     4441 cgcccaagtt aaaggcagac agtgtacaca gggcgatttc gccgttcgga tcgttgatat
     4501 tgtccagcgg tttggtcggc agggcgattt ccatacacaa gttggactga tgaacaggcg
     4561 cgacgcgcgg atcgaacggg ctgtgcgtat tgcagtgatc gacgttttga atgtagatgc
     4621 gcccggttcc ggcacgctcc tgcatcagcg tggaaaacag gtcggcagcc ggaatgatgc
     4681 gcttgcggat atcagggtct tgctcgtatt tcgtatagag ccgctcaaat tcgtcttggt
     4741 cggcaaaaaa cgcttcgtac aatcccggaa cctcgttggg cgaaaacagc gtaatgttgc
     4801 cgcccttaat caggcgggtg tacagcaggc ggttgatttg cacgccgtaa tcaagctgac
     4861 ggatacggtt gtcttccacg ccccggttgt ttttcaacac cagcaggctt tcggcttcga
     4921 tatgccacaa ggggtagaac aaggttgccg cgccgccgcg cacgccacct tgcgaacagg
     4981 atttgactgc cgcctgaaac attttaaaga agggaatgca gccggtatgc cgtgcttcgc
     5041 cgccccggat ttcgctgtcc agtccgcgga tacgtccggc attgatgccg atgcccgcac
     5101 gctgggaaac gtatttcaca atcgcgctgg tggtggcgtt gatggaatcc aggctgtcgt
     5161 cgcattcgat caatacgcag cttgagaact ggcgcgtagg cgtacgcacg ccgctcataa
     5221 tcggggtcgg cagcgatact ttaaatgtgg aaacggcatc gtaaaaccgt ttgacgtaat
     5281 cgaggcgtgt ctctttggga tatttgctga aaagacacat cgccaccaga acatataaga
     5341 actgcggtgt ttcataaatc tggcgggtaa cgcggttctg taccagatat ttgccttcga
     5401 gctgtttgac agcggcatag gaaaaggaca tatcgcgttc gtggtcgata taggcgttca
     5461 gctcgtcaaa ttcttcgcgg ctgtaatcct caaggatatg cctgtcgtat tttgcggcat
     5521 cggtaagttt tttaacgtgg tcgtaaaggt gcggcggctc gtactcgccg taggctattt
     5581 tacgcagatg gaaaatcgcc aaacgcgcgg caaggtattg gtagtccggg gtatcttccg
     5641 aaattaaatc ggcagcggct ttgatgatgg tttcgtggat gtcgtcggtg cggatgccgt
     5701 tgtagaactg gatgtgcgat ttcaactcga cctgagatac ggaaacattt tccaatccgt
     5761 ccgccgccca agtgacgaca cggtgaatct tatccaaatc aatggcttct aatcttccgt
     5821 ctcgtttggt tactttcaaa tcagtcggtg tattcatcgc ttcctcttcc actcttgata
     5881 ttcaagacac agtcttttca aataaattaa ggcagacaat atagtggatt tttggcattt
     5941 tctccagtct tgacaacggt tgtatttttc agtttcaggc aatgcgcgat aaaacccgcc
     6001 tgtcgtattt ttcctataat atttgtttta tctgataatt ctttaccgat aaaaaacggg
     6061 taaatttttt gccttttgac cggctccggc tacaaggcgg tgaaataagg attttccgac
     6121 gaaaaaggaa agcttcctgt tttctgcccc tgcaaattgt taaattttgc aaagtatgat
     6181 tttgccgcgc cgccgccgac aaattccatt ttcttaccga ttggaattta ttattgagat
     6241 taatgtgtta tttgaatctg catatcaaac ggcaagtttt gtcggctgaa tgagtctgaa
     6301 actgccgaca atttgcccgt tccatccttc catcctatac tggaaagaat gacaaactga
     6361 aaggatcgtc atgtctgtca aaaagatgct tgccatactg ttgtctgcaa tattgggact
     6421 ggtatcgaca actgccgctg ccggtacgtc agaacccgcc caccgccata ccaaacatat
     6481 ccgcaaggca aacaagcaga tgctgcaccc cgaatgcagg aaatatttgg aacgccgtgc
     6541 cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc gaaaacaaaa aggcgcgcaa
     6601 agcattccgc tccctgcctt atgcggaaca gaaaatccaa tgccgggcgg cttatgaggc
     6661 tttcgatgat ttcgacggcg gcagtttccg ccgttaatcc catataaaat atgccgtctg
     6721 aacacaggtt cagacggcat tttccataga tacaacaaac catccttacg ccatttcgtt
     6781 tgaggtactc ccgttgctgc agccttaata tagtggatta acaaaaatca ggacaaggcg
     6841 acgaagccgc agacagtaca gatagtacgg caaggcaagg cgaggcaacg ctgtactggt
     6901 ttttgttaat ccactataca tccgcacatc cgcaaaagcg aatcatactg ccacattccg
     6961 acaaccggtt tcagacagcg atatccgcat cgtcggcgac aacggcacgg atgctttctt
     7021 cgattttatc ttttaaagca taacggtctt cgctttccgc cgcatccgcc acgcaaacga
     7081 aatcgactct tatcgtcaat tttttcatag acacgatgcg ccacaggcag gtcggcaaac
     7141 cgacatcggc atatgaggga cgagccgtcc tttttcccgt ttcgtcataa taacgcagcg
     7201 cgaccgccaa aacctttgcc cccgcatcga tggcggattg gaacagcgcg gctttgaacg
     7261 gcaaaagccc caatccggag gaagtccgcg cttcggggaa aaaactgacg ttttgaccgc
     7321 gttgcaaggt ttcgcagacg gcgcggttaa tcggttcgat gtcgcgccgc gaattgcggt
     7381 tgatgaacac cgttcccgcg ttctgcccca tcttgcccaa taccggccag cttttgattt
     7441 cctgcttggc gataaagctg ctcggataaa ccgcgctcat cgcgaaaata tccagccagg
     7501 acacgtggtt ggcggcaacc aagacaccgt tcggatgttc gggtgcgggc ctgcccacct
     7561 ccaatccgat atccaaagcc gccaaaaccc ccctgcccaa ctcgattacc gcccgattgc
     7621 gcgactcggg gcaaccgccg tcaataccgc gcaggttttt cccggttttg aacagccaga
     7681 ccgccaaacg gcacaagcgg cgcagacgtg taaaaaatga agctttattt gaagacatga
     7741 gcaattcttt cccgataatc ggtgtttgtt gttgaaacgt tcggacggca taccgataaa
     7801 atgccgtctg aaaccgtttt cagtcctatt tctgacagtt cgggcaataa aacgtgccgc
     7861 gctgccccaa agtttctttc acaaccaaac cgccgcactg ggggcacggc tgattgtgcc
     7921 gcccgtacac tgtatattcc tgttggaagt agccgctttt gccgtcgctg tccacaaaat
     7981 ccctcaaggt actgccgccc gtttcaatgg cgcgctgcaa caccgctttg acggtttcaa
     8041 ccaaaagcgc acactctttc tttttcaggc ggttggcagg acggtggggc gaaatgcccg
     8101 ctctgaacag gctctcgttg gcataaatgt tgcccacacc gaccacgacc gcattgtcca
     8161 tcagggcaag tttgagcgcg cgtttctgcg ccttcagcct cacatacaga taatccgtgc
     8221 aaaatgcctc cgacaaaggc tccggcccca gtttttccaa cagcggatga tgttcttcga
     8281 ttccctcata ccaaagtatc gcgccgaact ttctcggatc gcggtaacgc atgaccgtgc
     8341 cgtctgaaaa cacaatatcg acgtgatcgt gtctgtccgg cctgccgata cgtccgtccg
     8401 acggcgtaaa aatccgcaag ctgcccgaca tccccaagtg aatcagcagc acgcccgttt
     8461 gaaagcggat aagcaggtat ttcgccctcc tgccgcagga caacacctgc cggccggaca
     8521 aaatctcccc caaatcggga ttaatctgcc agcgcagctt caattggcgc aataccacgg
     8581 cttccaccgt tttcccttca atatgcggcg cgatgccgcg caacgtcgtt tccacttccg
     8641 gcaattcagg cataacccct cccgacattt cttctgacag atgccgtctg aaagacggct
     8701 gtccctaatc cgcaaccctt gccgcacccg ccgcaagggc tttgccgccc aaatacccgt
     8761 cccactcggg caggggcgtt acgcccgccg cccttttgac caaaaccaaa ccgtacactg
     8821 cggcacattg cggccaccaa cggtcgcccg ccttttccat aaaccgccaa aagcgtattt
     8881 gcccgatcga cgaaacgggc ggcagataca ccataaattt cccaaattca atatcgaaac
     8941 cgacatccgc aagctgcctt ttcaactcgg gcagcggcag acaaaaccgt ttttccggca
     9001 ggcgttcgcc gtcaaaccaa cggctgaatc cccagagcga atacggattg aaacccgtca
     9061 gcatcaaacg tccgcacggt ttcaatatcc ggtgcgcttc cgacaggatt tgcgaaggga
     9121 taccgccttc aagcgtatgc gggaaaagca gcatatccgc agaaacatcc gccaaagcca
     9181 tattttccgc cgacatcgac atatctcgcg gcacacagac aacatcttca gacaggctca
     9241 gccacggacc gcccacctga accgcacaca ttcccgaaaa acggtatgaa tccagatacc
     9301 gcccgaagaa atcctgttcc aattttgcaa cataccgccc catcgccgta tcttcaaacc
     9361 atgaatccat attgcgtccg tttcaaacag attgcctgcc gatattctat tccaaacagg
     9421 atttctgtca aaaaacacat cggccgccca tttccgaatc cgcataaagt tcctgtaaaa
     9481 cttgacgctt tttcagtcaa acagtaccat cggacgataa aatatgttta ttccgccgaa
     9541 tataaatcat gtccaaactc aaaaccatcg ctctgaccgc atcaggtctg tccgtttgtc
     9601 cgggtttcct atacgcccaa aacacctcat cacaccaaat cggtttggcg attatgcgct
     9661 taaactcttc aatactcgac ctgcccccga caaaacaata tttccaatcc ggcagcctgt
     9721 ggagcgagct gcgccaaggc ttccggatgg gcgaagtcaa tcccgaactg gtacgccgcc
     9781 acgaaagcaa attcatcgca agccgcagct atttcaacag ggtcatcaac cggagtagac
     9841 cctatatgta ccatatcgcc aacgaagtca aaaaacgcaa tatgcccgcc gaagccgccc
     9901 tgcttccctt catcgaaagc gcgttcgtca ccaaagccaa atcacacgtc ggcgcatcag
     9961 gattatggca gtttatgccc gctaccggca ggcattacgg cctggaaaaa acaccggttt
    10021 acgacggcag gcacgacgtt tacgccgcca ccgatgccgc actcaactat ctgcaatacc
    10081 tctatggact gttcggcgac tggccgcttg cctttgccgc ctacaactgg ggtgaaggca
    10141 acgtcggacg cgccatcaac cgcgcccgcg cccaagggct cgaaccgacc tacgaaaacc
    10201 tgcgtatgcc caacgaaacg cgcaactatg tccccaagct gctcgccgtg cgcaacatta
    10261 ttgccactcc ccaatctttc ggcatgaata tcagcgacat agacaacaaa ccctattttc
    10321 aggcagtcga accggaccgt ccgctcgaca acgaagccat cgcccggctt gccggcatca
    10381 cgcaaagcga gctgctcgcc ctaaaccccg cattcaacgt ccccgcgttt atccccaaaa
    10441 gcaaacgcaa actgctgctt cctgtcgcgt ccgtacaaac cttccaaagc aactacctca
    10501 acgccgcacc cgacagcctg ttttcatggg aagtctatac gcctgccgcc aaaaccagct
    10561 tgtccgacat ctcgacggca accggcatga gcattgccga catcaaacgc ctcaacaacc
    10621 tgaacggcaa ccttgtcaac gcaggacgca gcatccttgt cgccaagaac ggcaagaccc
    10681 ttcatacggc atcggaatcc gtcgtttcca tcgacatcga caatacaccc gacacctacc
    10741 gttccaatat gccggcaggc acggtgaacg tcggcattgc ccgaatccga cccgccgccg
    10801 cacagacagc ggacattacc gtcgcacctt tgccgcagaa aaccgttcgt acggaacccg
    10861 atccccttgt ccgtattgcc gaacctgccc ttgcgacagc cgcagcgcaa cctcaaaccg
    10921 aaaaacagac cgccatgccg tctgaaaccc aaaccgcaac actcgcgcag atcgtccccc
    10981 aaaacgacat gcaggcggca gacgaactca tgcagcttgt tgcccgaaac aacctgcgcc
    11041 gacaggctga agaaaccatc tccgccgtca tcggcacgcc tgacacagtt gccgaacaca
    11101 acatttcctc atctccgcaa cataccgttg ccgccgacgg caaacgccgg acacgtttgg
    11161 aaacgcgcgt agccaaagct gccgacggcg aagccgaaat ctccccgctc catgccagca
    11221 tccaccgcgt tgtagaaggc gacaccctgt tcaacattgc caaacgctac aacgtcagtg
    11281 tagccgacct gattgtcgcc aacaacatca aaggcaacac catccaaaaa ggacaggtac
    11341 tccgcctggc gcaggcagcc cctgcccaaa cccgtattga aaaagtatcc tacaccgcgc
    11401 gcaaaggcga caccttcaca agtatcgccg cgcgcttcaa tatccatatc gacgacatcc
    11461 gccggctcaa tcccaacctg aacaccatca atccaggaca gagggttaaa ctgattggaa
    11521 gctgattcgg atacggcaca tgacaggact ttctcagtcc tgtctttttc atcccacatt
    11581 tcccatacca tcatgaaact catcaaatac ctgcaatatc aaggcatagg aagccgaaag
    11641 cagtgccaat ggctgattag gggcggctac gtctccatca acggaacctg tatggacgac
    11701 accgatgcag acatcgattc ctcatccgtc gaaacgttgg atattgacgg ggaagcagta
    11761 accgtcgttc ccgaacccta tttctacatc atgctcaaca agcctgaaga ttacgaaact
    11821 tcgcacaaac ccaagcacta ccgcagcata ttcagtctgt tccccgacaa tatgcggaac
    11881 atcgatatgc aggcggtcgg caggctggat gcagatacga ccggcgtatt gctgattacc
    11941 aacgacggca aactgaacca caacctgact tcgccgagca gaaaaattcc caagctatac
    12001 gaagtaacgc tcaaacaccc cacaggagaa acgctctgcg aaaccttgaa aaacggcgtg
    12061 ctgctccacg acgaaaacga aaccgtttgt gccgccgatg ccgttttgga aaacccgacc
    12121 accctgctgc tgaccattac cgaaggaaaa taccaccaag tcaaacgcat gatcgccgcc
    12181 gccggcaacc gcgtgcaaca ccttcatcgc cggcgattcg cacatctgga aacagaaaac
    12241 ctcaaacccg gggaatggaa atttatcgaa tgtccaaaat tctgaaataa catccaagaa
    12301 ttccatttat attcatccac atactcatta aatatatggt ttaacccaat ttaacgcaaa
    12361 aaatcaattt acgatataat ccatttgtct gagtaacgcc tgttcaagca ggcttatgag
    12421 taagacgttt tccccgtaat gtgttttgcc atctatactt ctccccttgt atagatggtt
    12481 ttttatttca aattccctct ctttcatcat tattaaaatg ctatcattaa tgtgattaac
    12541 aaaatttcat ttccatacac tgatgtagaa gaataacaaa ttaatctatt tataaactcc
    12601 gattcttcct catcgggtta tactcatcag aaatactcac tgaaatatat gccttttaca
    12661 tggaaaatca ctagacagtt gaaaaacaat cagtcaattc ctgttaaaaa acgcgtgtag
    12721 aatttccgct ttatctcaat cggacacggt ttcaatatgt cttccccttc aaataccaat
    12781 cgtcaaacct gatccagccg attaacctat atcctgaccg ttgccggcgc gactgtcggt
    12841 ttcggcgcga cgtggcgttt cccgtatttg gtcggtgaaa acggtggtgg cgcgtatgtg
    12901 tttttattct gtatcgcgat gctggttatc ggcattccga tgattttggt ggaaaatgtc
    12961 atcggacggc gcaaaggcgt gaacgcgctg gatgcgttcg gcggtccgat gaacggcaaa
    13021 cccattgcta aaatttggaa actggtcggc tggatggggc tgctcggcgc gttcggcatc
    13081 atggcttatt acatggcact cggcggctgg gtaatcagct atatcgttaa tattattgga
    13141 ggaaatttga atatttccag ccccatcgac ggtgtggtta caaaaggctt ctttgccgaa
    13201 cacattgaaa cagcccttgg gaaattgcgt tttatacgct gctttttgtc gccgtgaacc
    13261 aatggatttt ggtcaaaggc gttatcggcg gcattgaaaa agcggcaaaa tagctgatgc
    13321 cgctgctgtt tttgttccta atcgcgatgg tcgtccgcaa cgttaccctt ccgggcgcaa
    13381 tggaaggggt tactttctat ctgaaacctg atttcagcaa gattaccgcc gaactgttcg
    13441 tcttcgtttt ggggcaggta ttttttgccc tgagcctggg tttcggcgtg atgattacct
    13501 tgtccagcta tttggataaa aacgaaaatc tggttcagac ggcagttatc acggcaatta
    13561 ccaataccat catcgccata cttgcgggct ttatgatttt cccgtcgctc ttcagcttcg
    13621 gcgttgcccc cgattccggc ccgactttgg tgttccaaag cttgccgatt gtgttctcac
    13681 atatgtgggc gggatctgtg ttcgccgtga ttttcttctc gctgctcctg attgccgcgt
    13741 tgacaacttc gctgaccatt tatgaagtgt tgattacgac cattcaggaa aaaaccaaaa
    13801 tccgccgtac cgccgcgatt acgattgtat tggctgccat cttcattttc ggcaacatcc
    13861 cgtccattct gagctatggt ccgtggaaag acgtttccgt gttcggcaaa aatattttcg
    13921 atgccttcga ctacatcagc ggcaacatct tgtttatgct gaccgcgctc ggttccgcgc
    13981 tgtttgccgg ttttgtgatg aaggacgaag cgaaggacga attgctttat aaaggcaacc
    14041 atacgacggt caatatttgg tttgcttatg tgaaatatct tgtgccgctg gtgattctgc
    14101 tgattttcgt cagcaacctg ttctaatctg cagcaatcga tgccgtctga aggtcatacc
    14161 tctttcagac agcattgcct tgatgcccgc cacaataccc gcaacgccgg aatccgaacc
    14221 ggcttgccgg cttctgtcgg atgttttcgg gcaggcggcg ttccgtctgc ttagacaatc
    14281 gtcctttaaa acaggtagaa tccgccccaa cgggaaacac atccttcaga cggcaaaacc
    14341 cataccccaa accatcagga atccccctta tgaacagaca aaaagtcatc gccatcgacg
    14401 gcccgggcgc atcgggcaaa ggcacggttg ccgcccgcgt tgccgccgca ttgggatacg
    14461 attatctcga taccggcgca ctctaccgcc tgaccgccct atatgcacaa aaacaaggcg
    14521 tgggatggca cgatgaagaa aacgtttccg aactggcaaa aaaactgccc gccgtatttt
    14581 caggcagccg catcctgctc ggcggcgaag acgtttcaga cggcatccgg acagaagcca
    14641 tcggcatggg cgcatccgca gtcgcacagt tgcctaaagt ccgcgccgcc ctgctgcaac
    14701 gccaacgcga ttttctgacc gaaaaaggac tggtcgccga cggacgggac accggatcgg
    14761 tcgtcttccc ccaagccgaa cttaaaatct tcctgacggc agaatccaaa atccgtgccg
    14821 aacgccgcgc caaacaaatc ggcatcccct gcgaaggttt ggcattcgag cgcatcctgt
    14881 ccgacatcga agccagagac gaggcagacc gaaaccgcaa ggttgccccc ctgaaacaac
    14941 aacccgatgc cctgcttttg gatacgagcc gcctgactat agaagaaact gtaaaaaaag
    15001 tgcttgattg gtatcgtaaa gtttaaattt tcaggtataa tcgcccgcat tgcgtttcag
    15061 acagcatgaa tttccccata tgccgtctga aaccttatgt acccgtctgc cctgccaagg
    15121 acggcagaca cccaccaacc caccccgcac cccttggcgg tgtaccgaaa agagttatat
    15181 atgtctatgg aaaattttgc tcagctgttg gaagaaagct ttaccctgca agaaatgaac
    15241 ccgggtgagg tgattaccgc tgaagtagtg gcaatcgacc aaaacttcgt taccgtaaac
    15301 gcaggtctga aatcagaatc cctgattgat gtagctgaat tcaaaaacgc tcaaggcgaa
    15361 attgaagtta aagtcggcga cttcgttacc gttaccatcg aatccgtcga aaacggcttc
    15421 ggcgaaacca aactgtcccg cgaaaaagcc aaacgcgctg ccgattggat tgccctggaa
    15481 gaagccatgg aaaacggcaa catcctgtcc ggcatcatca acggcaaagt caaaggcggc
    15541 ctgaccgtta tgattagcag catccgcgca ttcctgccgg gttctttggt cgacgtacgt
    15601 cctgtaaaag acacttctca cttcgaaggc aaagagatcg aattcaaagt gatcaaactg
    15661 gacaaaaaac gcaacaacgt cgttgtttcc cgccgcgccg ttctggaagc cactttgggt
    15721 gaagaacgca aagccctgct ggaaaacctg caagaaggct ccgtcatcaa aggcatcgtt
    15781 aaaaacatta ccgattacgg tgcattcgtt gacttgggcg gcatcgacgg tctgttgcac
    15841 atcaccgatt tggcatggcg gcgcgtgaaa cacccgagcg aagtcttgga agtcggtcag
    15901 gaagttgaag ccaaagtatt gaaattcgac caagaaaaac aacgcgtttc cttgggtatg
    15961 aaacaactgg gcgaagatcc ttggagcggt ctgacccgcc gttatcctca aggcacccgc
    16021 ctgttcggca aagtatccaa cctgaccgac tacggcgcat tcgtcgaaat cgaacaaggc
    16081 atcgaaggtt tggtacacgt ctccgaaatg gactggacca acaaaaacgt acacccgagc
    16141 aaagtcgtac aactgggcga cgaagtcgaa gtcatgattt tggaaatcga cgaaggtcgc
    16201 cgccgtatct ctttgggtat gaaacaatgc caagccaatc cttgggaaga atttgccgcc
    16261 aaccacaaca aaggcgacaa aatctccggc gcggttaaat ccattaccga tttcggcgta
    16321 ttcgtcggtc tgcctggcgg catcgacggt ttggttcacc tgtccgacct gtcctggacc
    16381 gaatccggcg aagaagccgt acgcaaatac aaaaaaggcg aagaagtcga agccgtcgta
    16441 ttggcaatcg acgtggaaaa agaacgcatc tccttgggta tcaaacaact ggaaggcgat
    16501 ccgttcggca acttcatcag cgtgaacgac aaaggttctt tggttaaagg ttccgtgaaa
    16561 tctgttgacg ccaaaggtgc tgttatcgcc ctgtctgacg aagtagaagg ctacctgcct
    16621 gcttccgaat ttgcagccga ccgcgttgaa gatttgacca ccaaactgaa agaaggcgac
    16681 gaagttgaag ccgtcatcgt taccgttgac cgcaaaaacc gcagcatcaa actttccgtt
    16741 aaagccaaag atgccaaaga aagccgcgaa gcactgaact ccgtcaatgc cgccgccaat
    16801 gcgaatgccg gcaccaccag cttgggcgac ctgctgaaag ccaaactctc cggcgaacaa
    16861 gaataaggtt gcagacatga caaagtctga gttaatggtt cgtttggcag aagtgtttgc
    16921 cgccaaaaac ggcacgcatc ttctggcaaa agacgtagag tacagcgtaa aagtcttggt
    16981 tgacaccatg actagatcgc ttgcccgagg tcaacgcatc gaaatccgcg gtttcggcag
    17041 cttcgatttg aaccatcgtc ctgcccgcat cggtcgcaat cccaaaaccg gcgagcgtgt
    17101 ggaagtacct gaaaaacatg taccccactt caagcccggt aaagaattgc gcgagcgggt
    17161 cgacttggct ttaaaagaaa atgccaatta aaccttagca tcaaaacgcc gctgttacgc
    17221 ggcgtttttt ctgtggttta acttcatccg ctgcttcaat accttgagcc aagcaagcaa
    17281 cggattagag cgtggatttt tttatagtgg attaacaaaa accagtacgg cgttgcctcg
    17341 ccttagctca aagagaacaa ttctctaagg tgctgaagca ccaagtgaat cggttccgta
    17401 ctatttacac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatca
    17461 ttgcttacaa tccgcttttt aaacaacaaa tttttgattt ctattacgaa caggacaaaa
    17521 atcctgctta ttgcactaaa actaagccgt ttcaggaatt tgcggcaaat ttacggcttt
    17581 taccgaacct aatgctttcg ctttttggta aaacgccaat ttgtattcaa gcaaatctaa
    17641 atagcgtttt aattcggcaa tttgacactt cacattttct atttgatttt caaacaagga
    17701 aaggcgttct tcaatggtat cgtcgccaat gacggtacat tccgcaaagc gtttgatgtc
    17761 ttttaagctc attcccgtat ttttcaagca ttgcaataag cccaaccatt gcaaatcgtt
    17821 atcggtaaaa cagcggttac cgtattcatc acgtccgata ttaggcaaca agccttcttt
    17881 gtcgtaaaaa cgtagggtgt gggcggagat gcctattttt tcggcggctt tggcagtggt
    17941 ataagtcatt ttccctcctt ctaaacaaaa acagtaaaaa acacttgcct tagagttaac
    18001 tctaaagtgt aaactgttgc tatgttgctc aggtaaggca actttgtcaa tgaattaaga
    18061 ggaaagacaa tggaaatgaa acaaaccgat tcaaccatca aatctcgtgc ggcggtggca
    18121 ttcgccccta accaaccctt acaaattgtg gaaatcgacg tagaaatgcc tcgcaaaggc
    18181 gaagtgttaa tccgcaacac ccacacgggt gtgtgccata ctgatgcatt tacattgtca
    18241 ggaagtgatc ctgaaggcgt atttcctgtg gtgcttggac acgaaggtgc gggagtggta
    18301 gttgctgttg gtgaaggtgt gttaagcgta aaaccgggcg atcacgtcat tccgctttac
    18361 actgcagaat gtggcgaatg tgagttttgc ctctcgggta aaaccaactt atgcgtctca
    18421 gtgcgtgata cgcaaggtaa aggcttaatg ccagacggca caacacgttt ttcttatcaa
    18481 ggtcagccga tttatcacta tatgggttgt tccactttca gtgaatactc agttgttgca
    18541 gaagtttcac tggcgaaaat taatccagaa gctaatcacg aacaagtatg tttgctcggc
    18601 tgcggcgtta ccactggtat tggtgcggta cataacacgg caaaagtgca agaaggcgac
    18661 tctgttgccg tgttcggatt gggagcgatt ggtttggctg tgctacaggg tgcgcgtcaa
    18721 gccaaagctg gccgcattat cgctattgat actaatcctg ccaaattcga gttggcaaaa
    18781 cagtttggtg caacggattg tttaaacccg aacgattacg ataaaccgat caaagatgtg
    18841 ttgttagaca tcaacaaatg gggcattgat cacacctttg aatgtatcgg caatgtaaac
    18901 gtaatgcgtc aggcattaga aagcgcacac cgaggttggg gacaatccat tatcattggc
    18961 gtagcaggtg caggacaaga aatttcaact cgtccattcc agttggtaac aggccgtgtg
    19021 tggaaaggtt cagcatttgg tggtgtgaaa ggtcgctctg aacttccgaa aatggtggaa
    19081 gattcaatga aaggcgacat ccagttagaa ccgtttgtga ctcacgcaat gccacttgat
    19141 caaattaatg aagcctttga gttaatgcac gaaggtaaat cgattcgcac tgttattcat
    19201 tactaaggta cgctatgaaa ctgattgaac aacatcaaat ttttggcggt ttgcagcaag
    19261 tttgggctca tgatgcccaa acgcttcaat gcgagatgaa atttgccgtc tatttgccaa
    19321 ataattcgga aaatcaaccg cttggtgtga tttattggct ttctggctta acttgtactg
    19381 aacaaaattt cattactaaa tcaggctttc agcgttatgc agcagaacat caagtgattg
    19441 tggttgcccc tgataccagc cctcgcggag agcaagtgcc gaacgatgcg gcttacgatt
    19501 taggacaagg tgcgggcttt tatcttaatg caactgagca gccttgggcg acgaattatc
    19561 aaatgtatga ttatatcttg aacgaattgc ctgatttggt tgaagcaaat ttctctacta
    19621 acggcaaacg ttccattatg gggcattcaa tgggcggaca cggtgcattg gtattggcac
    19681 tgcgaaatcg gaaacgttat caaagcgttt ctgctttttc gcctatttta tcgccaagcc
    19741 tcgtgccttg gggagaaaag gcctttactg cttatttagg ggaagatcgt gaaaaatggc
    19801 agcaatatga tgccagctcg ctcattcaac aaggctataa agtgcaaggt atacgcattg
    19861 atcagggctt ggaagatgag tttttgccga cacaattgcg taccgaagat tttatcgaaa
    19921 cctgtcgtgc ggcaaatcag tcgatcaatg tgcgctttca caaaggctac gaccatagtt
    19981 attacttcat cgccagtttt attggcgagc atattgctta tcacgcggca tttttgaagt
    20041 aagccaaaga gcgttcagtg ttcaaagcag ttttgggata gccggcacga gggcggtaag
    20101 aagtgccggc ataaacgtat gccgtctgag ccggaagaag ccgactctac gga
//
LOCUS       DAWGJV010000021        19789 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWGJV010000021 DAWGJV010000000
VERSION     DAWGJV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.021"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(203..278)
                     /locus_tag="WHQ94_000540"
     tRNA            complement(203..278)
                     /locus_tag="WHQ94_000540"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WHQ94_000541"
     CDS             complement(371..730)
                     /locus_tag="WHQ94_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ2127870.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WHQ94_000542"
     CDS             complement(753..1397)
                     /locus_tag="WHQ94_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ2127871.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WHQ94_000543"
     CDS             complement(1399..1935)
                     /locus_tag="WHQ94_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ2127872.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WHQ94_000544"
     CDS             complement(1947..3044)
                     /locus_tag="WHQ94_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ2127873.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WHQ94_000545"
     CDS             complement(3047..4483)
                     /locus_tag="WHQ94_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ2127874.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WHQ94_000546"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WHQ94_000546"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ2127875.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WHQ94_000547"
     CDS             complement(5473..6120)
                     /locus_tag="WHQ94_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ2127876.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTGNTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELTALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQYRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WHQ94_000548"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WHQ94_000548"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ2127877.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVYL"
     gene            complement(7294..7431)
                     /locus_tag="WHQ94_000549"
     CDS             complement(7294..7431)
                     /locus_tag="WHQ94_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ2127878.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7495..8571
                     /locus_tag="WHQ94_000550"
     CDS             7495..8571
                     /locus_tag="WHQ94_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ2127879.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WHQ94_000551"
     CDS             complement(8778..10244)
                     /locus_tag="WHQ94_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ2127880.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WHQ94_000552"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WHQ94_000552"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ2127881.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGSEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYENG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WHQ94_000553"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WHQ94_000553"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ2127882.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WHQ94_000554"
     CDS             complement(13523..13807)
                     /locus_tag="WHQ94_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2127883.1"
                     /translation="MKKLLLAAVISLSAAAAFAGDHAERQIYGDPNFEQNRTKAVKML
                     EQRGYQVHDVDADDHLGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WHQ94_000555"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WHQ94_000555"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ2127884.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGGFPRMPYAEA
                     MFYYGSDKPDMRIGLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15824..16525)
                     /locus_tag="WHQ94_000556"
     CDS             complement(15824..16525)
                     /locus_tag="WHQ94_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ2127885.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAIKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="WHQ94_000557"
     CDS             complement(16668..17816)
                     /locus_tag="WHQ94_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ2127886.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGRDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WHQ94_000558"
     CDS             complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WHQ94_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ2127887.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18542..19684
                     /locus_tag="WHQ94_000559"
     CDS             18542..19684
                     /locus_tag="WHQ94_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2127888.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5104 a         5125 c         4570 g         4990 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg atcgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaat aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccacat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaagaccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcttta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg tgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatga gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgtg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcgctgtc caatatcggt actgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcggtaagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg gaaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgagtat ttcccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 gcggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggtata ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacaat cgtattctgg atgtgttttt gggtactgtc ggcggtctga tgggcaggca
     6241 ataataaccc tgcctttgca gatattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gagcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgaccct aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tagatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgca
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 agatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcgccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtcccg ggggcgaacg acgggtcgag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac ttggggggcg gtcaacacag taaaaggcaa ttttttactg tgttcggtac
    10801 gtatgccgat ttgcacggaa tgtgccggat cgatgatgcc ttccttgggg gcggtataaa
    10861 acatcgtgcc gtggtcgtat tcgctgccgt tttcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggg agggtgatga
    10981 aatggtcgcc gcccaagctc aaacagcgtt tgccggaaga gagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaaa tcgccgcagt
    11101 caataatgtt caggcgttcg cgtacgtcaa atgtccacgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt cagaaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa tgacccaatc tgcttggctt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tggtgattgg tgtttgagga agaaaatcgg gcttcagacg gcatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcagcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggcg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ctcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaagcagtt tgattgtgtt gcgccggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcatcttt ctatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccaaaacag
    13621 gtttgcccaa atggtcgtcg gcatcgacat cgtgaacctg ataaccgcgc tgttccaaca
    13681 ttttcacggc ttttgtgcgg ttctgttcaa aattgggatc accgtaaatc tgacgctctg
    13741 catggtcacc ggcgaatgca gcagcggcac tcagggaaat aacagcggcc aataacaatt
    13801 ttttcatttt cagtcctttc tttatcgact tattgaacaa gaagtgtttt tcaatgccgc
    13861 tattaaaaca cagccaaatt aggttcaaat tagcaaaaag ttaaaaagcg taaaatgccg
    13921 tctaaaaaaa cagagccgtc aaacggctct gttttcctta tgcttctttg gtttcagcca
    13981 ccttttggcg caggcgcagg ctcaattcac gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgt gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggcgcc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaagg ccgccgtgag
    14161 gaggcgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgcacgtc tgcgcggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcacgagcc aggcagtttg
    14341 cagggtcgga aaccatcagg tcttcatgac cttcttttgg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaagtcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aagtagccgt tatctttgcc gtgttccaag ccgactttga
    14521 tacgcaatgc gccgatggct tcgttcacga ctttggcttt gtccgcaccg aagaagatga
    14581 tgtcgccgtt ttccgcgccg gtacgctcga tgatttcttt cagggcattc tcagacagga
    14641 attttacgat tggagattgc aggccgctgt cttcgccgtt ggagaggttg cccacatcat
    14701 ttactttgat gtatgccaga cctttcgcac cgtagatgcc gacaaattta gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgcaccgt tcggcacacg cagggcaacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtaaa tttcaagccg atacgcatat ccggtttgtc agaaccgtaa tagaacatcg
    14941 cttcggcgta aggcatacgc ggaaaaccgc ccaagtccac gcccagcgcg tctttgaaga
    15001 cttgtttcgc cataccttcg gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgagtgaat tcaggctgac ggtcggcacg caagtcttcg tcacggaaac
    15121 atttggtaat ttgatagtaa cggtcgaaac cggccaccat caacagttgc ttaaataatt
    15181 gcggtgattg cggcagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttccggagtg gagcgggtca gcatcggcgt ttcgatgtcg atgaagcctt
    15301 gcgcgtccag ataacggcga acgcccatag cgacttgata acgcaggcgc aggttgcgtt
    15361 gcatcaccgg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtag cggcagcgtt caacacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tgtcgttggt cgtaccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcgagc ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgccgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcactg atcagaccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcaggcggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca acgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaatcgca ttcgacacct
    16081 gaccggacac gaatgcgatt gtccaaatac ccgattgggg aaacggcacg agtactggtg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggactt cacaaccgga atccgcccca acaagctgtc ccacgcggca agaatctgcc
    16261 ggcccaatac gtttgcggca aataatccgg ttacaaacaa tacagcaatg gcaacgataa
    16321 cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttac
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccag
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccctgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccgtcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaacc gcaccctttc cgccatttcg
    17881 ggattttgcc cccgcaatac agaaaacccc gaaaccgtca ggcttcaggg ttttccgctt
    17941 atcgcgtatc aaccgccttg gcggttttgc aaaaatcaag ccagtgcttt tacttttgca
    18001 gacaaacggc ttttgtggcg agccgctttg tttttatgga acacgccctt gtcggcgatg
    18061 cggtcgatga ctttgacgga ctcttggtaa accgcttgtg cggcagcttt atcgcctgct
    18121 tcgactgctt tcaatacttt tttcactgca gtgcggaatg cggtacgcag gctagcgttg
    18181 tgggcgcgtt gtttgacgga ctggcgggca cgtttgcgtg cttgtgcgct gtttgccata
    18241 ttggaatctc ctggaagaaa aataaattct gtaaacgcgc aattttaaag ggggtttaca
    18301 ttatatgcaa gccttttctc tctatccgcc cgtcggacac cctgacagcg caaaaaaacg
    18361 gcaaaatcgg gcggccgttt tcctcccctt ttagaaacag gataattttt taggaaaatc
    18421 agtccaatat cgctttgagt ttgcacaaat ttactttaca atgcccaaac cttgcgccgg
    18481 ggtaacgatg gggcgcgggg atttcttttt ttccaacctc tttcatcagg aatctgcccg
    18541 tatgaaaaaa tccgtattag ccgttttggc cgcgctgtct ctggcagcct gcggcggcag
    18601 cgaaaaaaac gccgtgcaac ctcaagcaga cgctgcgtct gctgccaatg ccgaagccgc
    18661 tgccaccgat actctgaaca tctacaactg gtcgaactat gttgacgaga gtacggtcga
    18721 agactttaaa aaagccaaca atctgaagct gacttacgac ttgtacgaaa acaatgaaac
    18781 gctggaagcc aaaatgctga ccggcaaatc cggttacgat ttggttgtgc cgggcatcgc
    18841 cttcctgcct cgccaaatcg aggcgggcgc atatcaaaaa gtcaacaaag acttgattcc
    18901 caactataaa aacatcgatc ccgaactctt gaaaatgctg gaaaccgccg acccgggcaa
    18961 ccagtatgcc gtcccctatt tctccggcgt aaacacgatt gcgattacgg cgaagggcaa
    19021 agagcttttg ggcggcaagc tgcccgaaaa cggctgggat ttgctgttca aacctgaata
    19081 cacccacaaa ctgaaatcct gcggcatcgc cctgtgggac accccgagcg aaatgttccc
    19141 gattttgctg aactacttgg gcaaagaccc caaaggctcg aatcctgaag atttgaaggc
    19201 ggcggcggaa gtgttgaagt ctatccgtcc tgatgtcaaa cgtttcagcc cgtccatcat
    19261 cgacgagctg gcgcgcggcg acatctgcct ggcggcaggc aacggcggcg atttgaactt
    19321 ggcgaaagca cgttccgaag aagtgaaaaa caacgtcggc atcgaagtgc tgaatccgaa
    19381 aggcatgggc ttctggattg agtcttggct gattcccgcc gatgcgaaaa acgtcgccaa
    19441 tgcccacaaa tacatcaact acacgctcga ccccgaaatc gcggcgaaaa acggcatcgc
    19501 cgtaaccttc gcccccgcca gcaaaccggc gcgcgaaaaa atgcctgccg aactggtgaa
    19561 cacccgttcc atcttcccga acgagcagga tatgaaagac ggtttcgtga tgccgcaaat
    19621 gagtacggat gcgaaaaaac tgtctgtcag cctgtggcag aaaatcaaag tcggcaccaa
    19681 ctgatttgaa gcattaaaaa tgccgtccga acatcgttcg gacggcattt tatattggat
    19741 tgaaatagaa atatttatat agtggattaa caaaaatcag gacaaggcg
//
LOCUS       DAWGJV010000022        19576 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWGJV010000022 DAWGJV010000000
VERSION     DAWGJV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.022"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            138..572
                     /locus_tag="WHQ94_000560"
     CDS             138..572
                     /locus_tag="WHQ94_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ2127889.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(620..1963)
                     /locus_tag="WHQ94_000561"
     CDS             complement(620..1963)
                     /locus_tag="WHQ94_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ2127890.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIRLANDSPFGLGACIYTADTGRA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1968..2621)
                     /locus_tag="WHQ94_000562"
     CDS             complement(1968..2621)
                     /locus_tag="WHQ94_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ2127891.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2772..3062
                     /gene="gatC"
                     /locus_tag="WHQ94_000563"
     CDS             2772..3062
                     /gene="gatC"
                     /locus_tag="WHQ94_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ2127892.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFAMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3125..4570
                     /gene="gatA"
                     /locus_tag="WHQ94_000564"
     CDS             3125..4570
                     /gene="gatA"
                     /locus_tag="WHQ94_000564"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ2127893.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLVPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMADFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4567..5499
                     /locus_tag="WHQ94_000565"
     CDS             4567..5499
                     /locus_tag="WHQ94_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ2127894.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5542..6972
                     /gene="gatB"
                     /locus_tag="WHQ94_000566"
     CDS             5542..6972
                     /gene="gatB"
                     /locus_tag="WHQ94_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ2127895.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKDQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITALRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7064..8074
                     /locus_tag="WHQ94_000567"
     CDS             7064..8074
                     /locus_tag="WHQ94_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ2127896.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSSRAVHFYLGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            8117..8623
                     /locus_tag="WHQ94_000568"
     CDS             8117..8623
                     /locus_tag="WHQ94_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127897.1"
                     /translation="MILSLKEKIEGIGNNVDHILLTTLILKWLHDYPGLEKEFLDHRS
                     NISEFMNIEILDIPNYPIKKSLISFYDLKALKRQIDISMQRNNPYIYQSLQNFLENLF
                     IIQEPDMECNICHGTYGFDLWKEIETNRLVYCCKTCAFAEYVNGEFRNNENHLTIPTN
                     DDLSLLNT"
     gene            8678..9190
                     /locus_tag="WHQ94_000569"
     CDS             8678..9190
                     /locus_tag="WHQ94_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127898.1"
                     /translation="MTNRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKKT
                     KRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKKL
                     IIDELLTERSRKLGGGVFGAGGKYSMHIYDFYLSEGEYLFEISDNSEYIPLYDEINNS
                     IRIVVNARIQ"
     gene            complement(9387..10019)
                     /gene="pdxH"
                     /locus_tag="WHQ94_000570"
     CDS             complement(9387..10019)
                     /gene="pdxH"
                     /locus_tag="WHQ94_000570"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ2127899.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            10250..11299
                     /locus_tag="WHQ94_000571"
     CDS             10250..11299
                     /locus_tag="WHQ94_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ2127900.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKTRPKGETRKTAAQAY
                     GQKVSDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(11368..12909)
                     /locus_tag="WHQ94_000572"
     CDS             complement(11368..12909)
                     /locus_tag="WHQ94_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ2127901.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAIKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(13202..14557)
                     /locus_tag="WHQ94_000573"
     CDS             complement(13202..14557)
                     /locus_tag="WHQ94_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ2127902.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAEERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            14754..15575
                     /gene="nadE"
                     /locus_tag="WHQ94_000574"
     CDS             14754..15575
                     /gene="nadE"
                     /locus_tag="WHQ94_000574"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ2127903.1"
                     /translation="MMRIFSTGGTMDTQAVITHIARWLDEYATQANAKGFVIGVSGGI
                     DSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(15646..16119)
                     /locus_tag="WHQ94_000575"
     CDS             complement(15646..16119)
                     /locus_tag="WHQ94_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2127904.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYHNVHRAQFEELVPLAQKVVQV
                     HADTFPAEIARLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            16417..16749
                     /gene="trxA"
                     /locus_tag="WHQ94_000576"
     CDS             16417..16749
                     /gene="trxA"
                     /locus_tag="WHQ94_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ2127905.1"
                     /translation="MSSELIIHTSDAAFEKDVLNSDTPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(16802..17749)
                     /locus_tag="WHQ94_000577"
     CDS             complement(16802..17749)
                     /locus_tag="WHQ94_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2127906.1"
                     /translation="MTDITPFANRLGKNIKHLMKWVKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMLDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            18077..19465
                     /locus_tag="WHQ94_000578"
     CDS             18077..19465
                     /locus_tag="WHQ94_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ2127907.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         5056 a         5508 c         4874 g         4138 t
ORIGIN      
        1 gggaatctgt tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact
       61 ttgaaccgaa cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa
      121 ccatcaagga aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt
      181 tcctgcccgt cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca
      241 gctattcgga tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga
      301 cgcgccaaac caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg
      361 gcgcggcaca ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa
      421 agaaaagaat tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa
      481 tgaacctgaa ggcggtggga aactcaaatg cgaaaaacaa ggacgatttg attcggaaat
      541 acaataacgc cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc cgatgccgtc
      601 tgaagcggca cgggtttgtt tatttctgcc agtaggtttt gacgttgacg aactcgtaca
      661 gcccgaattc ggacaattcg cgcccgtaac cggaatcttt aacccctccg aaaggcaggc
      721 gcaaatcgct gctggtatgg cggttgataa acaccgatcc cgcctgtatt ttttcggcaa
      781 accgccaggc gcgtccggta tcggcggtat aaatgcaggc accgagcccg aacggggaat
      841 cattggcaag gcggatggca tgttcttcgt tttcggcgcg caaaatcagg gcggccggcc
      901 cgaatacttc ttctctccag acgcggcagg caggatttac cctgtctaaa accgtcgcgg
      961 gataaaacca gcctcgccct tgtgggattt ttccgccggt caggcatacc gcgccgtttg
     1021 aaacggcatc ttcaacctgc ccgtgaaccc tgtcccgcaa atcttcgcgg tgcagcggtg
     1081 caagcgtggt atcgggatgt ttggggtcgc ccattttcaa tttagcgcat tcggcaagaa
     1141 acagcgtgat aaaacgatcg gctgcggctt cggttacgat gatgcgcttg gcggcgttac
     1201 acgattgccc cgcatcgcgg aaacgggaat aacaggcttc tgcggcggcg cgttccaaat
     1261 cggcatcggg catcacgata aaggcgttgc tgccgccgag ctccaacacg gttttcttga
     1321 ggtttgcgcc cgcatgtgcc gcaaggatgc gccccgtatg cgtcgagccg gtaaacgcca
     1381 tcgcatcggt atcttcaacc gccttgagcg tgcccgcctc atccaaccac gcgcctgcca
     1441 aaggaatgcc gtctgaagcc aaatcgaaca acgcctgact gacgcgtgcc acgctgggcg
     1501 cgggtttgac ggcgcacgcg ttgcccgcgc acatagcggg aacggcgaaa cgcaatacct
     1561 gccagacggg atagttccaa ggcatgacgg caaacaccac gcccaaaggc tcaaacctta
     1621 cctgactcaa actcgcctgc gtcgcgatgg ttttgtgggc gagcagttcg ggggcaaggc
     1681 gggcgtaata gcgtatcagt tcgatagact taccgatttc cgcgcggcat tcgtgcaggc
     1741 agcgaccgac ttcctcgcac accatttccg caaaacgctc tttctccgcc tccagacggt
     1801 cggcaaattt ttgcaggcgc gcggcacgtt cggttacgcc cagttgcgcg aacgccccgc
     1861 cgcgcatttt caaatccgcc agccgccgtt caaactccgc ataatcttga gcggggcggc
     1921 ggtaaagcgt ttcgcccgta aatacattga cactgtgaaa catcgaatca acctgccagt
     1981 tgcgggaata tcgttttcag tcccgacaca ataatctcca ccgataccgc cgccagcatc
     2041 atacccataa tgcggtttaa aatcgtcagc cccgtcgcgc ccagcaggcg gctgaccttc
     2101 ccggcaacga ttaaaatggc ataacaaatc gcactgacca ccaaaccggc cgcaataatc
     2161 aacgcgatgt cgccgtatgt tttagccgcc gaagcgtaaa taatcacggt cgaaataccg
     2221 cccgggccga tggtgatcgg tatggcgatg ggcacgacag caatcgctcc ggcattgcgg
     2281 gcggggcgcg cctgccccgt ttccggctgc gcgccgagat tctgcttggc gggattgtcg
     2341 ttgccgttca tcatagaaat ggcaatcagc aacaccaaaa ttccgcctcc gacctgaaac
     2401 gaaccgacgc tgatgcccaa aaccttcaac agcgtaccgc cgatcagcgc aaataccgca
     2461 atcacggcaa acacggcaac ggcggccgtc cgcgcgacct tcctgcgctc cttcgtgctg
     2521 tgcccgttgg tcaggtcaag gtaaagcgac aacgcgctaa acggattaat cagcaccaaa
     2581 aaagccacaa tcagcttgcc gatttccatg cccaatccca ttatttcccc ctccttcaaa
     2641 cccgtgcggc aggcatccga tgctgcaaat tgccgccgca acggattttt ccgttataat
     2701 taaaaattca agcaatacgc cccatcatac ccgaacgacg gtatctttac catcagacaa
     2761 ggatgctttt catggcactg acacttgccg acgtagacaa aatcgcccga ctctcccgac
     2821 tgcacctgac tgcggaagaa aaagaaaaat cgcttcaaga attaaacgac attttcgcta
     2881 tggtcgaaca gatgcagaac atcaacacag acggcatcga accgatggcg catccgcacg
     2941 aggccgccct gcgcctgcgc gaagacgaag taaccgaaac cgaccgcgcc gccgaatatc
     3001 aggcggttgc tccggaagta cgcaaccgtc tgtacatcgt accgcaagtt atcgaagaat
     3061 aatccgaata tgcttcagac ggcatcagca ataccgcccg aagcccttta aggatggaag
     3121 atttatgacc caatacacat tgaaacaggc aagcgtcctg ttgcagtcca aacagatttc
     3181 cgccgtcgaa ctggcaagcg cataccttgc cgccatcgcc gaaaaaaatc ccgccctcaa
     3241 cggctatatc accatcgacc aagataaaac ccttgcagaa gcccgtgccg ccgacgaacg
     3301 catcgctcag ggcaacgctt cggcacttac cggcataccc gtcgcctaca aggatatttt
     3361 ctgccaaacc ggctggcgca gcgcgtgcgc ttccaaaatg ctcgacaact tcatctcccc
     3421 ctacaccgcc accgtcgtcc aaaacctgct cgacgaaggt atggtaacgc tcggccgcac
     3481 caatatggat gagttcgcta tgggttcgac caatgaaaac tcattctacg gtgcagccaa
     3541 aaacccatgg aatcttgagc acgtccccgg cggttcgtca ggcggttccg ccgccgtcgt
     3601 tgccgcgcgc ctcgtccctg ccgcgctcgg ttcggacacc ggcggctcta tccgccaacc
     3661 cgcatcgcac tgcggcatta ccggcatcaa acccacatac ggcacggttt cccgcttcgg
     3721 tatggtcgcc tacgcctcca gcttcgacca ggccggtccg atggcgcaaa ccgccgaaga
     3781 ctgcgcgatt ctgttgaacg cgatggcaga tttcgacccc aaagactcca ccagcctcga
     3841 acgcgaaaaa gaagactaca cccgcgattt gaaccaaccg ctcaaaggcg tgaaaatcgg
     3901 cctgcccaaa gaatacttcg gcgaaggcaa cagcgccgat gttctgacgg cattgcaaaa
     3961 caccattgat ttgctgaaag cccaaggcgc ggaattgatt gaagtttccc tgccgcaaac
     4021 caagctgtcc atccccgcct actacgtcct cgcctccgca gaagccagca ccaacctttc
     4081 acgttacgac ggcgtacgtt acggacaccg tgccgcccaa ttcgccgatt tggaagaaat
     4141 gtacggcaaa acccgcgccg aaggtttcgg cagcgaagtc aaacgccgca tcatgatcgg
     4201 cacttatgta ctgtcgcacg gctactacga tgcctactat ctcaaagccc aaaaactgcg
     4261 ccgcctcgtt gccgatgatt ttcagacggc atttgcacgg tgcgacctca tcctcgcgcc
     4321 gaccgcaccc actgcagccc caaaaatcgg agcggatgct tcgccggttg aaacctactt
     4381 gagcgatatc tacaccatcg ccgtcaacct cgccggactg cccgcattga ccctgcccgc
     4441 aggcttcagc ggcggcggac tgcccgtcgg cgttcagctt gtcggcaact acttcgccga
     4501 agccaaaatc ctcggtgcgg cgcatcaaat ccaactcaac agcgattggc acggcaaacg
     4561 acccgaatga agcagaaccg cacctttacc ttccccgatt ttcgcaccgt ttacagctat
     4621 gcgcctttat atcggctgca acatttaaaa tacacattgc gaaaattttt cggaaaaaaa
     4681 gaaatttacg ccttcgagca gtttgtcaac gcctccccta tccgtcaggg gctgttcctc
     4741 cactgcccgc aaaatgccta tccgctgctg cgcgaatttg ttgacaggcg ttttaactgc
     4801 aaacgccgtt tagatgcgat gacggcagat tttctcatgg cggaaaaact gttcggcaca
     4861 gacatcctgc accaaatgga agactaccgc ttccatttgg tcttggcgca cctttcagac
     4921 ggcatcagct tgtggctcaa ccgcaacgac aactgcgtcg aagaaggcgc gtggtcttta
     4981 tctttgcgcg acgaagcagg caaccggctg tatatggcga ctttcgcctt tgtcggcaca
     5041 cacctgctga cagcctccgt acaagggcct gcgggtgaag aagccaaaga cactgtccgc
     5101 cgcataacca aacaacttca cggcttgcgt ccccaacaac tgatggtaac cgcactgcaa
     5161 tatttcgccg ccgtactcgg cttggacggc gcaatgggca ttgcacaaaa acatcaggtc
     5221 aaactgcgct ggaaacttaa aaagcgcgtc aaaatgaatt acgacgcatt ctggcaggaa
     5281 tacggcgcaa gtttggaacg ggacggctac tggcatctcc cccaaacccc cgcccgcaaa
     5341 gaccttgccg acatcgaaag caaaaaacgc tcgatgtacc gcaagcgtta tgaaatgctg
     5401 gacaacatgg ttgcagagat gaaggacggt ctgaaaacag aagcacgcgg catttcagac
     5461 ggcatccaaa cggaaaaacc gctccgccgg acagcctgac gcgaagacta tcgaattgat
     5521 attttagaga aagaagctct tatgacctgg gaaaccgtaa tcggcttgga aatccacgtc
     5581 caattgaaca ccaaatccaa aatcttcagc ggcgcatcga ccgcattcgg cgcagaaccc
     5641 aacgcgcacg ccggcgtagt ggaatgcgcg ctgccgggcg tactgccggt aatgaaccgt
     5701 gaagtcgttg aaaaagccat caaattgggt ttggctttag atgcgaaaat caaccggaaa
     5761 aacgtgttcg accgtaaaaa ctacttctat cccgacttac caaaaggcta tcaaatcagc
     5821 cagttggatt tgccgattgt cgaacacggc aaattggaaa tcgtagtcgg cgacgatgtg
     5881 aaaaccatca acgtaacccg tgcgcacatg gaagaagacg caggcaagtc cgtacatgaa
     5941 ggcttgaacg gcgcgaccgg tatcgacctg aaccgcgccg gcacgccttt attggaagtg
     6001 gtgtccgaac ccgaaatgcg ctccgccgcc gaagccgttg cctacgccaa agccttacac
     6061 agcttagtaa cctggctgga catttgcgac ggcaatatgg cggaaggttc attccgcgtc
     6121 gacgcaaacg tatccgtgcg tccgaaagat caagcagagt tcggcacgcg ccgcgagatt
     6181 aaaaacctca attccttccg tttcttggag caggcgatta attacgaagc agaagcgcaa
     6241 atcgagattt tggaagacgg cggcaaagta cagcaggcaa cgatgctgtt tgatcccgaa
     6301 aaaggcgaaa cccgcgtaat gcgcctgaaa gaagacgcgc aagactaccg ctacttcccc
     6361 gaccccgatt tgctgcccgt catcatttca gacgctcaaa tgcaaaaagc caaagcagaa
     6421 atgcccgagc tgccgaaaga aatggcagcg cgtttcgtgg cggactacgg cgtgtccgaa
     6481 tacgacgcgc gcctgctcac cgcaagccgc gcacaggccg cctattttga agaagccgcc
     6541 aaagagagcg gacaaggcaa gccgactgcc aactggatga acggcgaact cgccgccgca
     6601 ctgaacaaag aaggcatgga acttgccgac agcccgatta ccgctctgcg cctcgccgcg
     6661 ctggtaggca aaatcgccga cggcacatta agcagcaaat tagcgaaaaa agcctttgag
     6721 gctatgtggg cggaacccga agccactatt gccgaaatca tcgaaaaaca cggtttgcaa
     6781 cagatgaccg ataccggcgc ggttgaagcg atggtggacg aagtgctggc aaacaacgcc
     6841 aaagccgtgg aacagtttaa atccggcaac gaaaaagccc tgaatgcgat tgtggggcaa
     6901 gtgatgaagg ccagcaaagg caaagccaat cccgcgcagg ttcaagagct gattaaagcc
     6961 aaactggcct gataagcaag atgccgtctg aaacccagat ccgacgttca gacggcattt
     7021 tcacagtctg accacattaa aacagcataa aaaacaatat tccatgaacc acaccgttac
     7081 cctgcccgac caaaccacct ttgccgccgg cgacggtgaa accgttttgg ccgctgccgc
     7141 ccgtcaaaac ctcaatctgc cccattcctg caaaagcggt gtctgcggac aatgcaaagc
     7201 cgaactggtc agcggcgata ttcaaatggg cggacactcg gaacaggctt tatccgaagc
     7261 agaaaaagcg caaggcaaga ttttgatgtg ccgcaccacc gcgcaaagcg atatcagcat
     7321 caacatcccc ggctacaaag ccgatgccct acccgtccgc accctgcccg cacgcatcga
     7381 aagtattatt ttcaaacacg atgtcgccct cctgaaactt gccctgccca aagccccgcc
     7441 gtttgccttc tacgccgggc aatacattga tttactgctg ccgggcaacg tcagccgcag
     7501 ctactccatc gccaatccgc ccgaccaaga aggcattttg gaactacaca tccgcaggcg
     7561 caaaaacggt gtctgctcgg aaatgatttt cggcagcgaa cccaaagtca aagaaaaagg
     7621 catcgtccgc gttaaaggcc cgctcggttc gtttaccttg caggaagaca gcggcaaacc
     7681 cgtcatcctg ctggcaaccg gcacaggcta cgcccccatc cgcagcatcc tgcttgacct
     7741 tatccgccaa gacagcagcc gcgccgtcca tttctacttg ggcgcgcgtc atcaagatga
     7801 tttgtatgcc ctcgaagaag cacaagggtt ggcatgccgt ctgaaaaacg cctgcttcac
     7861 ccccgtattg tcccgccccg gagagggctg gcagggaaga aaaggccacg tacaagacat
     7921 cgcggcacaa gaccaccccg acctgtcgga atacgaagta tttgcctgtg gctctccggc
     7981 tatgaccgaa caagcaaaga atctgtttgt gcaacagcat aagctgccgg aaaacttgtt
     8041 tttctccgac gcattcacgc cgtccgcatc ataattcccc ggtatagtaa aagggcagta
     8101 attatgcaaa aaaaaaataa tactttcttt aaaagaaaaa atagagggta taggtaataa
     8161 tgtagatcat atcttactca caactttaat tcttaaatgg ttacatgatt atcctggttt
     8221 agagaaagaa tttttagatc accgttcaaa tatttctgaa tttatgaata tagaaatttt
     8281 agatatccct aattatccaa taaaaaaatc actaattagt ttttacgatt taaaagctct
     8341 taaacggcaa atagatattt ccatgcaaag aaataatcca tatatttatc aatcgctaca
     8401 aaatttttta gagaatttat ttattattca agaaccagat atggaatgta atatttgcca
     8461 tggcacctat ggttttgatt tatggaagga aatcgagact aatagattag tttattgctg
     8521 caaaacatgt gcattcgcag aatatgttaa tggggaattt agaaataatg aaaatcacct
     8581 taccattcca acaaatgatg atttgagttt attgaataca taattaatag aataagttat
     8641 agaaaaccat gtttacattt ttcatcatga gaagcttatg actaatagaa aaatattatt
     8701 aataatatct tgcctattga ttgctagctg cagttttgtt gaaactattt tttatatggc
     8761 tattagccca gaacctgttg tggtagactt tcctcttggt aaaaaaacaa aaagatctat
     8821 tgaactcaaa cagaaaattg gtaaacctta tgcaatatcg ttaggaacta attttataca
     8881 ttatgatcca aaacaggggg agaggtggat tgatgataag ttaaactatc catataatat
     8941 atcggttaaa atatttaaag tggaagaaga tggtaaaaaa cttattatag atgagttact
     9001 tacagagaga agtagaaaat taggaggcgg agtatttgga gctgggggaa aatacagtat
     9061 gcatatttat gatttttatt tgtctgaagg ggaatattta tttgagattt ctgataatag
     9121 tgaatatatt ccactttacg atgaaataaa taattctata agaatagtag ttaatgcacg
     9181 aattcagtaa atttttctag aaatgtgggg ttacttatgg ctgattattt tgcgataact
     9241 gtattaaatc ttgttttaga taacctcaaa aagaggactc gggctttccg ttcggaacac
     9301 aaaaaacttc ctgtccgtgt tttcccgtga aaaaatgccg tctgaaaccc gattccggct
     9361 ttcagacggc atatgttttt tcctgtttaa ggcgacagtc gctcgcgtat ccagccgcca
     9421 tccagcaaac ggtattggat gcggtcgtgc agcctgctcg gcctgccctg ccaaaactca
     9481 atcaaatcgg gaatcacaat atagccgccc caatgcggcg gacgcggcac gtgcaaagga
     9541 tgtttgagtc cgaccgccgc cgcctttgcc accaataccg ccttgttcgg aataacctcg
     9601 ctctgcgcac ttgcccacgc acccaaacgg ctctgatacg ggcggctctc aaaatattcg
     9661 tccgacaact tctccgccag cctttcaaca cgcccttcca cgcgcacctg acgctccagc
     9721 tccggccaaa aaaacgtcat cgccgcaaat ggatgagcat ccagcgaacg ccccttgcgg
     9781 ctgtgataat tcgtaaagaa aacaaaacct tcagaattaa cttccttcag cagcaccata
     9841 cggctgttag gcctgccgcg tccgtcaacc gccgccacat tgaccgccgt cggctcgttg
     9901 acctgtgcgc gtaccgcctc gtccaaccac cgctcgaact gctcgatcgg attgtcggcg
     9961 caatctcctt ccgacaattc ccgtttactg taatcttccc gaatattgtg caaatccatt
    10021 taccgctcct cttgtcattt gaaagattct acccccgcac gcaaaccgat ttcaaccgtt
    10081 gcacaaactt tgcctcgctt ctccaaacct caggacacaa cgatttcatc cgcaaaaccg
    10141 ccgcataagg tacaatatcg aaccgtccga ccgagggcgg cattttatca acccgtcctg
    10201 ccgcacacgc cgcagaagaa ccgccttatc aggcgagtta ggaaaaatga tgtccaagca
    10261 acccaccagc aaacgccaat ggcgcgacgg cgcagccccg tctgccaaga aaaccgccaa
    10321 accgttcaaa agcaaaaccc gtcccaaagg cgaaacgcgc aaaaccgccg cccaagctta
    10381 cggacaaaaa gtttcagacg gcatcaagcc gcaaaacgcc cccaaacagc gcgccgccaa
    10441 agccaaaaaa ctcgtcgtcc gcaatcccaa ccaaaaaatt atggaacacg cgcgcgattt
    10501 gaaagaacgc cgcagcgacc tatcgcgcat ggaacccgaa cgcctgcaaa aagtgcttgc
    10561 cgcgtccggc gtcggctcac gccgcgaaat ggaagaatgg attaccaacg gctggataac
    10621 ggtcaacggc aaaaccgcgc aactgggcga caaagttacc cccgacgacc acgttaccgt
    10681 caaaggcagc atcatcaagc tcaaatgggc ggaccgcctg ccgcgcatca tcctgtatta
    10741 caaacaagaa ggcgaaatcg tttcccgcga cgacccgcaa ggccgcgtca gtattttcga
    10801 ccgcctgccg caggccgcca gcagccgctg ggtcgccatc ggacgcttgg acatcaacac
    10861 cagcggactt ctgattctca ccacctccgg cgaactcgtc caacgtttcg cccaccccag
    10921 cttcgaagtc gaacgcgaat acgccgtgcg cgtcttgggc gggctgaccg gcgaacaaat
    10981 gcgcgtcctc accgaagaag gcgtgatgct cgaagacggc ttggcaaaag tcgaacgcat
    11041 ccgcgaacaa ggcggcgaag gcgcgaacaa atggtacaac gtcgtgatta aagaaggccg
    11101 caaccgcgaa gtgcgccgca tttttgaaag ccaagggctc accgtcagcc gcctcgtgcg
    11161 cgtcggcttc ggtcccatcg gactgcccaa ccgcctcaaa cgcgggcagt tctacgaact
    11221 caaccccgcc gaagtcgcca acatcatcaa atgggcggac atgctgctgc cgggcgaacg
    11281 ccgccgcaaa aaagcctaaa cccgccaaaa cacaaaaatg ccgtctgaaa catctgctgt
    11341 ttcagacggc attttattcg ggcgttttca ggagaaaagg tcgagtgctt tgacaaagac
    11401 catcaccacg ccgtaggcga gcggtatgac ggccaatgcc caacgccacc agaccgatat
    11461 gccgccgccg gaaacttttt cgcccacaag gtaagcgtcg gatatggcgg tttcgtcatc
    11521 ggggttgccg ctgtgtgcgg cggttttgat gtctttttcg tggtgttttt cgtgtacgga
    11581 tttgacggcg aggttgcaca acaaaccgat aatcagcaga cccgccatga tgtacatggt
    11641 tacgctgtat gcctgtgccg ccggtatgcc gctgtcgatt tggctttggc gtatgtaatt
    11701 gaccagtacc gggccgatga cggcggcggt tgaccaggcc agcaggatgc gcccgtgaat
    11761 cgcgccgacc tgataggtgc cgaacaggtc tttcaggtag gcgggaatgg cggcaaatcc
    11821 gccgccgtac atggaaataa tcacgcaaaa gccgatgatg aacagggctt tgctgccgcc
    11881 ctcgccgata gagggaacgg cgaaatacag cagcgaaccg agtacgaaga agatggtgta
    11941 ggtgtttttg cgtccgattt tgtcggaaac gctcgaccac aaaaagcgtc cgcccatatt
    12001 aaacaggctc aggaggctga cgaagcctgc cgccgcacct gcgccgactg ccgcctgcct
    12061 acctacggag gtttcggaaa agagttcctg aatcatcacg gatgcctgac ccaatacgcc
    12121 gatgccggca gttacgttca ggcacaatac ccagaacaac agccaaaact gcggcgtttt
    12181 aatggcttgg gacacgttga catgattgct gctgaccagc ttgttttgcg ttttcggcgc
    12241 ggtatagcct tcaggtttcc agccgtcggc aggtacgcgg atggtaaacg cgccaaacat
    12301 catcagtgcg aggtaaagca gacccaatac ggcgaaggtt tcggcaaccc caaccgaagc
    12361 agcgtttgaa aaggcgttca tcagtgatac ggaaagcggc gaggccagca ttgcgccgcc
    12421 gccgaaaccc ataatcgcca aaccggtcgc catacccggc ttgtcgggaa accatttcat
    12481 cagtgtggaa accggcccga tgtagcccaa acccaagcct acgccgccga tgacgccgtt
    12541 gcccaaatag agcaggaaga ggttgtgcgt acgcacgccg aatgcggata cgaagaagcc
    12601 caggctgaag cagcaggcgg cggcaaatat ggctttgcgc ggccctaccc gttccatcca
    12661 cgtaccgaac agggcggccg acgcgcccag catcgcgagt gcgatactga aaatccaacc
    12721 tacggtcgtc agcttccaat ctccggccgc cgattcggtt atgccgataa gtttggtcag
    12781 cggcgcgttg aatacggaat aggcgtaaat ctgcccgatg gcaaggtgca ccgccaatgc
    12841 tgcgggcggt acgagccaac ggttgaaacc cggcttggca atgcttgcct cacggtctaa
    12901 aaacttcata acatcctctt tctgtcagtt gaaaaataaa atttcatttg ctcaatggaa
    12961 acttattgaa aactataaaa aaatatcgga tcggtttttt atccgcccca agatgcgccg
    13021 tctgaaacat ttcgggtgta cggaaaggtt tctgtttttt ccgacaaatt cctgcggctt
    13081 ttcgcttccg gattcccgct ttttcaggaa tgacgaatta aagattatct taaggtcaag
    13141 ggactggatt cccgctttcg cgggaatgac ggcggagggg gagcggtttt tccgattggg
    13201 tttaaatgca atcgaacaaa tcctgctgcc cttgttcttt gcttacgcgc acgtcggttt
    13261 cgccgtcggc gaaggtaatg tgcagcttct gcccctgctt caaaacatcg gcgttgcgga
    13321 tgacttgtcc gcgtgtatct ttgacgacgg aaaagccgcg ttccaaaatg tgctgcggcg
    13381 aaacggcttc gagcaatgcg gcttgggcgg tcaggctttg gcggcggtgg gtaagcagtt
    13441 ggctgaaggc ggcgggcaat gccgtctgaa ggcggacgat gttgtttttg caaacggaaa
    13501 catcaggacg gtaatgttgc agggcttggg tttggcgttc aaaacgggcg gtgtgggcac
    13561 ggaggttttg cttcatagag taagacagcg tttgcgccag cttgccgatt gaagcgcgct
    13621 gctcatcaag tttttggcgc ggatgacgga tttgccgcgc aagccagtcg agtttttggc
    13681 tggcatcgaa atagcgttgt tccaaaacgg ttttcagacg gccttgcgcc tgggcgagac
    13741 gatgtagcga ttcttggcgg ttggggctga ccagttccgc cgcgccggtc ggcgtgggcg
    13801 cgcgcacgtc ggcgacaaaa tcggcaagcg tgaaatcggt ttcgtgtccc acgccactga
    13861 cgacgggaac cgtgcaggct tcgattgcgc gtacgaccgg ttcttcgtta aacgcccaca
    13921 aatcttcaat gctgccgccg ccgcgacaga caatcaacac gtcgcattcg gcgcgttgcg
    13981 atgcggtttt aatcgcttgg gcaatctgaa actcgctgcc tgtgccttga acaggtgtcg
    14041 gataaacgat aacgggaatt tcgggcgcgc ggcgtttcaa ggtggttaca acatcgcgca
    14101 atgccgccgc cgccaaactg gttacgatgc cgatacattg agggcggacg ggcaacggtt
    14161 ttttgcgttc ctccgcaaac gcgccttccg cctgcaactg cgccttcaac cgctcatagg
    14221 cttcgtaaag ctgccccaaa cctttgagcc gcacttcgtt cacggtaatc tgaaattcgc
    14281 cccgcgtttc ataaatactg attttgcccg ccacttcgat atggtcgcct tctttcaaag
    14341 gtttcgccaa acgcgccgcc gcacccttga acatcgcgca acgcacctgc gcgcggctgt
    14401 ctttgagcga gaaataataa tgcccgctgg cggcacgggt caggttggac acttcgcccg
    14461 caatccacag accggcaagg tggttttcca aaatactttt ggcaaatgcg ttcaactcgg
    14521 aaacggacaa cacgtcagaa tggaaaaaat cagacatcga atcaatcaaa tagtaaaaaa
    14581 tatgaatatg ttttgaagcc caaggcggca tagagccgcc taaattgtca acaatattat
    14641 aacacgcgcc atcttgccgc ccgactttgc ccgtatgact tttttaagcg gggaatggga
    14701 aaaatattca tcaacctgcc tgcaatctat tcaaattgaa ccgccgacaa gctatgatgc
    14761 ggatattttc gacaggagga accatggata cgcaggcagt tatcacacat atcgcccgat
    14821 ggttggacga atacgccact caggcaaatg caaaaggatt cgtcataggc gtttccggcg
    14881 gcatcgattc cgccgtcgtc tccacactcg ccgcccgcac cggccgcccc acgctgcttc
    14941 tggatatgcc gatacgccaa caccccggcc agcttgagcg ggcaaggctg cacatccgca
    15001 atctgcaacg gcaatatgcc aatgtaagcg cgcaaacggc cgatttgacc gacaccttcc
    15061 agacctttga acaaaccgtc ggcattcatc agacggcatt tgacaatcag ccgctttccc
    15121 tcgccaacgc caaaagccgc ctgcgtatgc tgaccctgta ctactacggg cagatacacg
    15181 gactgctggt tacggggaca ggtaataaga ttgaagattt cggcgtggga ttttttacca
    15241 aatacggcga cggcggcgta gacatcagcc cgattgccga cctgaccaaa acgcaggttt
    15301 accggcttgc cgaagcattg ggcgtggacg aggcgattca aaaagccccg ccgaccgacg
    15361 gcctgtggga tacggaacgc accgacgaag aacagatggg cgcaagctat cccgaactgg
    15421 agtgggcaat gggcgtgtac ggcacgcgca aacccgaaga ttttgaaggg cggcagcgcg
    15481 aagttctgga aatctatacg cggctgcacc gcgcgatgca gcacaaaatc aacccgattc
    15541 ccgtgtgccg cattccgccc gaattgttgg gctgaaacgt ggaaatgccg tctgaagcgg
    15601 aaaaaccgta tttcagacgg catggaaata tccggctccc gttccttaag aatcgagtac
    15661 gcgggcaaac agaatatcgt tttccaaatg aatgtggtcg ttcaaatcct ccaccatttc
    15721 tttggccaaa gcgtaaaggc gcgtccagct tccgcaagcc ccttctggcg gttggaaatt
    15781 gtcggtcagc tctttgagcc gtgcgatggc gcggtcgtgt tcttcgtgtt cgtgcatcat
    15841 cacgctgatg ggcattgccg caccgcgtcc gacaccctga ttaatcatcg gaaacagcat
    15901 cctttcctct ttcatcatat gcatcagcag ttcgttctgc atatcggcaa gcagcctggc
    15961 aatttccgcc ggaaaggtat cggcatggac ttggaccact ttctgcgcca gcggcaccaa
    16021 ttcttcaaat tgtgcgcggt ggacattgtg gtagcgttgc aggatataat cgacggttgc
    16081 gccaaagggg gctgtttccc aaacggaaaa atcagtcatt gcagtgttcc ttttataggg
    16141 tttcgggttt ggttttgaac attcatactt taagaatcta tttaaacgga gcatacaccg
    16201 cccgcgcgtt tctgtacagc ttcaaaaaca ttccttacac tttgataata aaagtaattt
    16261 tcaaaaataa aatactgtcc gaaccgtttt ttagaatttg caaaggcgat tggggcggta
    16321 cagaaaaact attatcccgc ccgcccactt gaaattttta tgcccaagcc ctatcctgca
    16381 cgctatcgtg ccaatcccaa ccgaaaagga aaaataatga gcagcgaatt gattatacac
    16441 accagcgatg ccgcttttga aaaagacgtt ttaaattcag atacccccgt cctgctggac
    16501 ttttgggctc cgtggtgcgg cccctgcaaa atgattgccc cgattttgga cgacatcgcc
    16561 gccgaatttg aaggccgtct gaaagtggtc aaaatcaaca tcgacgacaa cgaagccacc
    16621 ccgtcccgtt tcggcgtgcg cggcattccg accctgatgg tgttcaaaaa cggcgaagtc
    16681 gtggctacca aagtcggcgc attggcaaaa ggtcagctga ccgcctttgt cgaagcctcc
    16741 atcgcctgat aaagcgcaat cgaaaaagcc gccggaagat tccggcggct ttttcgcacc
    16801 cttaagattt gtggcggatt tcccagcacc tatggatttt tttgttgcgg aaatcttcgg
    16861 gaacggattg tttggaaatg tctttgacgg cgtattgttc cgataccaag tcgtctaaga
    16921 cgaagctgcg caggttgttg gaaaagtaca aaatgccgtc tgaagcgagc agcttcaccg
    16981 cgccgtcaat cagctttttg tggtcgcgct ggatgtcgag aatgtcgagc atctttttgc
    17041 tgttggagaa gctgggcggg tccatcacaa tgaggtcgaa ccgcctgcct tccccatatg
    17101 ccgtctgaag atattgaaac acgtcggcgc ggacgatttt gtgtcgttcc gtatcgatgc
    17161 cgttcaattc aaaattgcgt ttcgcccaat caagatatgt gttggacaaa tcgacggttt
    17221 cgctggatgc cgcgccgccg gtggcggcat agacggtgaa gctgccggtg taggaaaaca
    17281 ggtttaaaaa acgtttgccc gccgccgttt cgccgacttt tttgcgcgtg tttcgatgat
    17341 ccaaaaaaag ccccgtatcc aaatatttat caaggttgac ccaaaacttg cggccgtttt
    17401 cggtgatgac gaaatcgtcg cccgccttgc cggttttctc gtactgctgc aaaccttttt
    17461 ggcgttcgcg gcgtttgagg cggatttgtt cgggcgcaaa accggtaacg aaagcgacgg
    17521 cttccaagac ttcggcaagc cacgcttcgt attcttcggg ccgcatcagc cagccggtat
    17581 cgtattcctg aaggtggatt cgatcgccgt aaacatcggc ggcaaagggg aattggggga
    17641 tgtcgcggtc gtaaatgcgc caggcttcga tgccgttgcg tttcacccat ttcataaggt
    17701 gtttgatgtt tttgcccaag cggttggcaa acggtgtgat gtcggtcatt ggtttcaggc
    17761 ggaataaagt ggaaaacggc aattttactg taattaacgc ccgattgctt gaccgtttcg
    17821 ggcaaaccct ataccatccg tcgcttatct tgtcatacga agccatcgcc ttccaaccta
    17881 aaccgccctt acgggcgcgt ttcttctgtt gctttgattt tgcaaagcat atctgtgcag
    17941 gttgccgtcg atgtaaacca caagcaagcc gcttgcgaca accctgtaac ttcacattcc
    18001 ccgtatcgtt acccttccct gcttcaggcc gtctgaacct ttcggacgcg ggcgttgttg
    18061 tcttccaagg atagccatgt ctattaaatt tgccgatttg aaccttgata aaaacatttt
    18121 gtccgccgtc agcagcgagg gttacgaaag cccgacgccg attcaggcgc aagccattcc
    18181 gtttgctttg gaaggccgcg acatcatggc ttcggcgcaa accggctccg gcaaaaccgc
    18241 cgcctttctg ttaccgactt tgcaaaaact gaccaaacgc agcgaaaaac cgggcaaagg
    18301 cccgcgtgct ttggtgttga ccccgacccg cgaactggcg gctcaagtcg agaaaaacgc
    18361 gctggcgtat gccaaaaata tgcgttggtt ccgcaccgtc agcatcgtcg gcggcgcgtc
    18421 tttcggctac caaacccgtg ccctgagcaa accggtcgat ctgattgtcg ccacgccggg
    18481 ccgtctgatg gacctgatgc aaagcggcaa agttgatttt gaacgtttgg aagtgctgat
    18541 tttggacgaa gccgaccgta tgttggatat gggctttatc gacgacatcg aaaccatcgt
    18601 ggaagcaacg ccgagcgacc gtcagacttt gttgttctcc gccacttggg acggcgcggt
    18661 cggcaaactg gcgcgcaaac tgaccaaaga ccctgaaatc atcgaagtcg aacgcgtgga
    18721 cgatcaaggc aaaatcgaag aacaactgct gtactgcgac gatatgcgcc acaaaaaccg
    18781 cctgctcgat catatcttgc gcgatgccaa tatcgatcaa tgcgtgattt tcacgtccac
    18841 caaagccatg accgaagtca ttgcggatga actgtacgaa aaaggtttcg ccgcaaactg
    18901 cctgcacggc gatatgccgc aaggctggcg caaccgcacg ctgatggatt tgcgtaaagg
    18961 ccgctgcaaa attttggttg ccaccgatgt tgccgcacgc ggtatcgacg taccgaccat
    19021 tacccacgtt atcaactacg acctgccgaa acaggcggaa gactacgtcc accgcatcgg
    19081 gcgcaccggc cgcgcaggcc gcacgggtat tgcgattacg tttgccgaag tgaacgaata
    19141 cgtcaaagtc cacaaaatcg aaaaatacat taaccgaaaa ctgcccgaac tgaccatcga
    19201 aggcatggaa ccgacccgca aacgcaaatc cgcaggcggc aagccgaaag gcaaaggcgg
    19261 ctggggcgat cgtaaatccg gcggttggcg cggcgatcat aaaccgagca aagaaggctt
    19321 cggcggcaaa acgcgcggcg aaggtttcaa gaaagaaggc tttaagagag acggtttcaa
    19381 aaaaaccggc gaaggcttca aaggcaaacg caaagccggc gattcttttg caggcaaagg
    19441 cgaacgccgt tacaaagacc gctaagcccc aacctgccgc ataaaccaat gccgtctgaa
    19501 accgatttcg agtttcagac ggcatttttg caatgtttca gcaccgcccg gctttgatac
    19561 ccaaaggatt aatctg
//
LOCUS       DAWGJV010000023        19362 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWGJV010000023 DAWGJV010000000
VERSION     DAWGJV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19362
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.023"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(100..1269)
                     /locus_tag="WHQ94_000579"
     CDS             complement(100..1269)
                     /locus_tag="WHQ94_000579"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ2127908.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1443..1553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1606..2502
                     /locus_tag="WHQ94_000580"
     CDS             1606..2502
                     /locus_tag="WHQ94_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ2127909.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLANVKGVKTLFFCCERLPDGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2546..3610)
                     /gene="tsaD"
                     /locus_tag="WHQ94_000581"
     CDS             complement(2546..3610)
                     /gene="tsaD"
                     /locus_tag="WHQ94_000581"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ2127910.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3727..4914)
                     /gene="ccsB"
                     /locus_tag="WHQ94_000582"
     CDS             complement(3727..4914)
                     /gene="ccsB"
                     /locus_tag="WHQ94_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ2127911.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4907..6859)
                     /locus_tag="WHQ94_000583"
     CDS             complement(4907..6859)
                     /locus_tag="WHQ94_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ2127912.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7136..7759)
                     /locus_tag="WHQ94_000584"
     CDS             complement(7136..7759)
                     /locus_tag="WHQ94_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ2127913.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7965..8594
                     /locus_tag="WHQ94_000585"
     CDS             7965..8594
                     /locus_tag="WHQ94_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ2127914.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8715..11111)
                     /locus_tag="WHQ94_000586"
     CDS             complement(8715..11111)
                     /locus_tag="WHQ94_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ2127915.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11263..12378
                     /locus_tag="WHQ94_000587"
     CDS             11263..12378
                     /locus_tag="WHQ94_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ2127916.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGKAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12381..12980
                     /locus_tag="WHQ94_000588"
     CDS             12381..12980
                     /locus_tag="WHQ94_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ2127917.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASENAQEAN"
     gene            12981..13628
                     /locus_tag="WHQ94_000589"
     CDS             12981..13628
                     /locus_tag="WHQ94_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ2127918.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13646..14191
                     /locus_tag="WHQ94_000590"
     CDS             13646..14191
                     /locus_tag="WHQ94_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ2127919.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14210..16495
                     /locus_tag="WHQ94_000591"
     CDS             14210..16495
                     /locus_tag="WHQ94_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ2127920.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQATAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17372..17884
                     /locus_tag="WHQ94_000592"
     CDS             17372..17884
                     /locus_tag="WHQ94_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ2127921.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            17964..19043
                     /gene="aroB"
                     /locus_tag="WHQ94_000593"
     CDS             17964..19043
                     /gene="aroB"
                     /locus_tag="WHQ94_000593"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ2127922.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5149 a         5555 c         4455 g         4203 t
ORIGIN      
        1 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatataa aaatgccgtc
       61 tgaactgttc agacggcatt tttgattttc aaaccggaat tacagccccg ctgccgccct
      121 caatgcagca gctttgtcgg tgcgctccca agtgaactca ggttcttcgc ggccgaaatg
      181 tccgtaagcg gcggatttac tgtaaatcgg gcgcaagagg tcgagcattt ggacgatgcc
      241 tttggggcgc aggtcgaaat gttcgcgaac taaggtaatc agtttttctt cgctgatttt
      301 gcctgtgccg aaagtatcga tggaaatcga agtcggttcg gcaacgccga tggcgtagga
      361 aacttggatt tggcattggg ttgccaatcc tgcggcgacg atgtttttcg ccacatagcg
      421 gcaggcgtag gcggcggaac ggtcaacctt ggacgggtct ttgccggaaa acgcgccgcc
      481 gccgtgcgga gccgcgccgc cgtaggtatc gacgatgatt ttacggccgg tcaaaccgca
      541 gtcgccttgc gggccgccga tgacgaagcg gccggtcggg ttgatcaggt atttggtttc
      601 gtcggtcagc agttcagacg gcaggacggg cttgataatc tgctcgatga cggcgttttt
      661 cagttcttcg taagcgatgg acggatcgtg ctgggtagag aggacgacgg tgtcgatgcg
      721 ttttactttg ccggtttcgc tgtcgtaaac cacggtcagt tgggcttttg cgtcagggcg
      781 cagccaaggc aggcggccgt ctttgcgtaa ttcgctttga cgctgcatca ggcggtggct
      841 gtaatagatg gcaaacggca tcagggtcgg ggtttcgtcg caggcatagc cgaacatcaa
      901 accttggtcg cccgcgccct ggttcaggtc gatgccttcg ccttcgttca cgccttgggc
      961 gatgtcgggg gattgctggt cgtagtacac gccgaccgcg cagccgttgg catcaaagcc
     1021 cagctcggac gagttgtagc cgatgcgttt gatggtttcg cgtgcgactt tgatgtagtc
     1081 cacttgggcg gtggtggtga tttcgcccgc caatacgcac aagcctgtgt tgaccaaggt
     1141 ttctgcagcg acacgcgctt tggggtcttg cgccaagatg gcatccaaaa tcgcatcgga
     1201 cacttggtcg gcaactttgt ccggatggcc ttcggatacc gattcggaag taaacagata
     1261 ttcgctcatt gctattcttt cattaaaaac aggctgctgt gttttcagac ggccttttat
     1321 ttcggacagc ggaaatcata acaaaaactc cgctttttct ctatacaagt gagaaacatt
     1381 cgcacttgtc tgctgtttcg gaatcctgtt gcgcgtttct tacaggcaaa aaaattcccg
     1441 cattaagcgg gtttggattg ctctggtgag ccacatcggc ttttcaaccg tccaccttac
     1501 ttttcctttt gaaaagcagg ttggcatgga attcccaact cttaatgcag cgcggatggt
     1561 agcagaaaag cctttccgcc gcaatacttc cctttttcag acggcatgtt tcgtttacaa
     1621 ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc tgttgaccgc cctgctcaaa
     1681 tgcctctccc tgctgccgct ttcctgtctg cacacgctgg gaaaccggct cggacatctg
     1741 gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg ccaatatgcg tcaggcaggc
     1801 atgaatcccg accccaaaac ggtcaaagcc gtttttgcgg aaacggcaaa aggcggtttg
     1861 gaacttgccc ccgcgttttt cagaaaaccg gaagacatag aaacaatgtt caaagcggta
     1921 cacggctggg agcacgtgca gcaggctttg gacaaacacg aagggctgct gttcatcacg
     1981 ccgcacatcg gcagctacga tttgggcgga cgctacatca gccaacggct gccattcccc
     2041 ttaaccgcca tgtataaacc gccgaaaatc aaagcgatag acaaaatcat gcaggcgggc
     2101 agggttcgcg gcaaaggaaa aaccgcgcct accagcatac aaggggtcaa acaaatcatc
     2161 aaagccctgc gttcgggcga agcaaccatc gtcctgcccg accacgtccc ctcccctcaa
     2221 gaaggtgggg aaggcgtatg ggtggatttc ttcggcaaac ctgcctatac catgacgctg
     2281 gcggcaaaac tggcaaacgt caaaggcgtg aaaaccctgt ttttctgctg cgaacgcctg
     2341 cctgacgggc agggtttcga tttgcacatc cgccccgtcc aaggggaatt gaacggcgac
     2401 aaagcccatg atgccgccgt gttcaaccgc aatgccgaat attggatacg ccgttttccg
     2461 acgcagtatc tgtttatgta caaccgctac aaaatgccgt aacgaaaata aaaatgccgt
     2521 ctgaacaatt tcagacggca ttttgtcatt tgacgatttc cgacagcggc cagcgcggac
     2581 ggacattgaa cgcaccgacc tccctgccct tgcccaggtg catcgcaccg gcaaaggcaa
     2641 tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa aaacacgctg actttttcgg
     2701 acggatgttt cggcttgcct ttgggggtcg ggatttgcac cgtcatgttg ccgaaagttt
     2761 cacggagctt gcggtttgca ccgaccccgc cggcgaccac tacggttctg aaccctgtct
     2821 gcaacagggc ttttttcact ttcgccgcca acacatcgac taccgcatct tgaaacgcac
     2881 ggcagatgtc gttgcgtgtc tgctcaggaa tgtcatccgc cccgttttcc gcgcgcactt
     2941 tctcgacggc ggtcaatacg gcggttttca aacctgaaaa actcatctgc aaatcgtcgg
     3001 aatgaatcat cgggcgcgga aaaacaaacg cttcgaacct gcccgattcc gcaagttccg
     3061 acagtttcgc accgcccgga tacggcaagc ccagcagttt cgccgttttg tcgaatgcct
     3121 cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta gtcgcctatg cccctgaccg
     3181 ccataatctg cgtatgcccg cccgaaacca acagcgcgac aaaaggaaag tcgggttttt
     3241 cctccgccaa cagcggcgac agcagatgtc cttccaaatg atggacggga ataacaggct
     3301 tgtccaacgc taaagccagc gcgttggcgt agctcgaacc cgccagcagc gcaccgccca
     3361 aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc atacgatgcg cccgcctgcg
     3421 ccaaacagcc ttcagtcaac ggaacaaggc ggcggatatg gtcgcggctt gccagctccg
     3481 gcacaacccc gccgtattcg gcgtgcattg ccatctgcgt gtgcaggcag tgcgcccgca
     3541 atcctcgttc ggtgtcatac agcgcaacgc ctgtttcgtc gcaagaagat tcgattccca
     3601 ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt attttagcgg atttcggcac
     3661 gactgccgta tcccaaaaac ggaacatgcc gtctgaagac cgttcagacg gcatcgtcgc
     3721 accgtatcaa agcgttccgt aagaatgcag cccgctcaaa aacatattca cgccgataaa
     3781 agcaaatgcg gttacgaaca aaccgataat cgcccaccac gccagcactt tgccgcgcca
     3841 accggcaacc agccgcaagt gcagccaaac ggcgtaattg agccagacga tgaacgccca
     3901 cgtctctttc ggatcccaac tccaatagcg tccccaagca tctgccgccc acagcgcacc
     3961 caaaatggtg gcaatggtaa agaacagaaa gccgacggca atcgccttat acatcacctc
     4021 gtcgatcaat gccgacggcg gcagccacag ttttccgcct tttccttccg cacgcaggga
     4081 aaccagttcg gcaataccga gcatcgcgga aatgcaaaac gcgccgtaac cgataaagtt
     4141 tgccggaacg tggattttca tccaccagga ctggagcgcg ggaatcagcg gctggatggt
     4201 atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca accacgaccg ccataaagcc
     4261 gaacacgaag ccgcccaatt tctgtatggc gaacttacct tcataataaa gatacatcag
     4321 cgcggtaatc accaaaaaca ggatgaacac ttcatacagg ttggaaaccg gaatatgccc
     4381 cgcatcggga cggagcagat agctttcgtg ccaacgtacc agcagaccgg taaagcctgc
     4441 tacggcagac acccatgcaa acacggttcc catacccaac agcgtgttgg tcggcacatt
     4501 ttttacgctt gccaaaaccg cgcccgaaat ataggcgaac agggcgaaga agacaaaggc
     4561 gcactgccac atgatcgccg actggctgct gaggaaatac cgcaacagga aaatctctgc
     4621 cgatttgatg ttgccgccat acaaaccgac ggcggcatag gcaagcaata cgcttaaagg
     4681 aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac acggcaatac cggcacttgc
     4741 ccacaacatg accgtttcgt aaatgtccat atgcataccg gaacgggtct gtacgaaaac
     4801 cgtagccgca aaaaccagca cggcaaatac ccaatcccaa agattcagat tgctgatcaa
     4861 agacttctga atcagcagct cgtgttccgg aagggtttta tagtgttcag tcatgattca
     4921 agtccttgcc gagccgttgc agactctcga cgtgttttgg aaattccttc tgcaaatccc
     4981 gttcgctgcg ggccgaagac atggcaaaac ggattttgcc gtctgaaaac aatacccacg
     5041 cccgtttttc gcgcacataa aacatcaata ccgtacccaa taccaacagc accgagccga
     5101 gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa acccgacgaa cgcacctcgg
     5161 aaaacccatc aagttgcagc agcataggcg cgggatattc ggtcaaaccc gtgtacgcat
     5221 ccatactgtg cagcaggaaa cgattccgcg cttcatcctg ctgccattcg ggcaagccgt
     5281 accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc gtaaagcatt tcgtagaaat
     5341 aaccctgcat cttatcctgc tgctctttcg ggatattgga cgtaataaat tcgtccaatc
     5401 ccaaatagcc tttttgtgca aagatgttca gcgtgttttc cgcagccagc atgaattgtt
     5461 cgcggatttc ggcaggtgcg cctttggttg cgtcggcaac cagacgtttg cgcccttccc
     5521 catctttcaa aaactcacgc aatgccataa aggtgtccgc tttcaactgc ttgtccaagg
     5581 ggatacgcag ccagcggtat tgctgctgca agccgctgcg cgtgccggta atccaaaaat
     5641 aatcctgttc ctgcaaaacc ggcagcatat agtttttata ttcgaccgcc tgccctgccg
     5701 catcacggat acggtaaaca atggaagggc cgatattggt gtatttttta ccttcctgag
     5761 taacggcgcg gacatcgttc agcgtggatt tcaggctttt ttcccgttcc gcgccctcgc
     5821 tcatgtcctc cacattcata gaagtgaact gatcgaactc aagacgatat ttgtgtttgc
     5881 caatttccaa cggaaactgg tgtatggatg ttgccttcaa cacgacaggc tcgcgcgaag
     5941 catcacccaa attccacgcc ttgaatgtca aatccgaacc gccgtcggca aaactcgcct
     6001 gataaatcgt gatgccgtgc aaggtcaaag gatggttcac gcggatggtg cgctcgagtt
     6061 tctcaccggt tgccttatcc gttacttcaa tatcactggc aaaatcgcgc ggcatacccg
     6121 tattgtaaaa atcgatatgg aattttttca gtttgacttc aaaaggcaag tcctgaacca
     6181 atatcccgtt gtcggcattc aggaaaacca catccgcact ctgcccctcg gaaatattga
     6241 cgttgcccct aaatgagaga ttggacgcac tcaaaatact ttcgggcttg aaatccttgg
     6301 cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag catacccagt ttcaacagca
     6361 ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa agcaacatgg gcaaagatat
     6421 agccccattt gttcatcgtg ccttttttgg cggcaatcag aaccgacccg ccctcacggc
     6481 tgacggtttt tccctgaaaa ccctgcacct ccagataacg tttggcaacc tcgggcgcaa
     6541 tttttacatc caacagcgaa gaatggcgca tcgccgccag agatttttct ttaacctttt
     6601 cccgaaaaga cttcatttcg cgccagaacg gcggcacatt gcgaatcagg cacaaactgg
     6661 tagaaaccac caaaaacatc atgataacga caaaccatgc cgaagcatag acgtcataca
     6721 gtcccagaaa accaaaaatc tgcgcccaaa aaggtccgaa tttgaccaaa taatccgtct
     6781 gcggctggtt ttgctgcaac accgtaccga taaccgatgc aatacccagc agactgagca
     6841 aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca cggacgggaa agaagtgggg
     6901 gagatctacg ggatttactc attgtgtgtt tattccgcca tcaggaatat gggaaagcag
     6961 aattgggcaa acagaaaaca acgtcccgat tctactgtct tgatgctttt tatttcaaga
     7021 caatgaagac agcctgcatc gattccaacg gttgcgattg aaaaaactta tcgcagaatt
     7081 gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa cagactattg cggaattaac
     7141 gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc ttcagacata cggtttgcaa
     7201 tatcttccat gatggtattt ttacgctgac cggacttgta ggcattcatc tgttcaacaa
     7261 tatatgcctg atgctgaccg cccaaacgcg gataagcctg aatttcgctt ccgcctcccg
     7321 gcatacccgc accgctcgga ccgtggcagg acatacacgc cggcactttt ttatcgctca
     7381 aaccgccgcg atagattttc gcacccaatt cgggattttc cttaggattg gcttcaccgg
     7441 atttgggctg ctgtttggca tagaatgcgg atacgttcaa aatatcctga tcgctcaaat
     7501 tcattaccac cggtttcatc acagctgccg aaccgtgggt gcgtttaccg tcgcggatgc
     7561 cgatggtttg atgatagatg taagcagtat gctgtgccgc caaacgcgga tacatcgcaa
     7621 tgccgctgtt accgtctgct gcatggcaag ccgcacaaac cgttgcggca acctgtttgc
     7681 ctttttccac gtctgctttg ggagaggcgg aaaccgcacc ggcagccaaa acaaaggcca
     7741 ataaagtcaa tcgtttcatg gagtgctcct gattacagca ttggataacg caacaatgct
     7801 ctttttatat tcaaatacgg gatttttgac ccgattaaaa ccgatgattc tgtaaacgtg
     7861 ttattctata ctaaatttac attaaattac cactgtgttt cacataaaac caaccgcata
     7921 tttttgctgt cggacaaacg gcggcggaaa acaaggatat gcccatgaac ctttttcaaa
     7981 acgccaaatt cttcacgacg gtcaaccacc ttaaagacct gcccgacacc cctctcgaaa
     8041 ttgcctttgt cggcaggagc aatgccggaa aatccagtgc catcaatacc ctgaccaacc
     8101 atgtccgtct tgcctacgtt tcaaaaacac ccggacggac acagcatatc aacttcttcg
     8161 agctgcagaa cggcaatttt atggtcgatt tgcccggcta cggttatgcc caagtccctg
     8221 aagcagtacg cgcacattgg gtcaatctgc tcggcgacta tctgcaacag cgcaaacagc
     8281 ttatcgggct ggttttgatt atggatgccc gccatccttt aaaagaactc gacatccgta
     8341 tgctggattt tttccacacg accggcagac cggttcacat cctgctgtca aaagccgaca
     8401 aattatccaa aaacgaacag ataaaaaccc tgtcccaagt caaaaaactg ctcaaacctt
     8461 attccgacag gcaaaacatc agcgtacagc tgttttccag cctgaaaaaa caaggtattg
     8521 acgaggccaa ccgaactgtc ggaagctggt ttgacgcagc agatgccgcc gcttcctctc
     8581 caaaggaaaa ctgaccccaa ttatacggaa accgtattcc ccccacttga ccgaccgcaa
     8641 acatttaaaa aattgccact gccaaatcta aaatgccgtc tgaaagactt ttcagacggc
     8701 attttgcgga gtctttaaaa cagagaatcc aactgctgct gtttggaacc agtattactc
     8761 ggaagcaccg gcgtttcctg catatcttgg cggacttcgt catccgccgc ctgccgtccg
     8821 ccttctgccg cgcccccgtc atcttctttt gcccgtcggg aaggttgcgg cgcaataccg
     8881 ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt ccttcatata gtattcgcca
     8941 ttgctgctga ccacaccttc aggcattttc atccccttgc cctgctttcc tttcaacgca
     9001 aaacgcatat agtccaccca aaccggcacc gcaatcgtac cgccgtagcc gacacgcccc
     9061 atactcttag gtttgtcgaa gccgatatat acggcagtaa ccacatcagg gttaaaaccg
     9121 acaaaccacg catccttatt gtcattggtc gtacccgttt taccggcaat atccgttctt
     9181 cccaacgcag ctgcccccct tgccgtacca acacggacca catcctgcat aatcttatac
     9241 ataatatagg cattgcgcgg atcgattgcc tgaggcgcat tttgcccggc caccaaaggt
     9301 tgcatttggg cgcgcaacct gccgtctctg tcataaatct tatcgattac gtgcgaagaa
     9361 accctatatc cgccgttcgc aaatacgcta tatgcctccg ccactttcaa cggcgttgtc
     9421 tcgcccgtac ctaaagccat agacaggctt gccggcagct cggacgacct gaagccgaaa
     9481 cgccggatat actgttgcgc gtaaccgaca ccgatagaca tcaaaatacg gatggaaacc
     9541 atattcttgg aagccgtcag agcctgtctc aaagtaatgt agccggaata tctgccgtct
     9601 gaatttttag gtgtccagac cgaaccgttc ggccctttcc ccggcaggga aatcggcgca
     9661 tcgttaacca ctgtggacgc ggtcatcccc ttagataatg ccgccgaata gacaaacggc
     9721 ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac gattgaatgt tttgctgtga
     9781 aaatcataac cgccgaccag cgcgcgcaca gctccggttt ttgcatccag cgaaaccaaa
     9841 gccccctgca gcaacggctc ttgaaccacc gcccaacgcc cgccgttgtt tttgacacgg
     9901 atgaccgcgc ccctgcggat acggtcctcc cccatttttt cattattgac cgcgcgggcc
     9961 gcaaagccca aggcgcgcct atcaagcgta acccgcctgc cgccgggcag ctgtatgacg
    10021 acatttttct ttttagtcac atccaacaca acggcgggaa ccattttatc gacggtatag
    10081 agtcccgaca gatactggct gacagtctcc tcgacatctt cactcttact caaatcgata
    10141 tagttttccg caccgcggta gctgctgccg cgatcgaaat tccgtagagc cttgcgcaat
    10201 gcctcggttg ccaccttctg atgatcggcg cggaccgtgg tataaacctt aaaaccctgc
    10261 gtataggcat cttcaccgta tttctcatac agttcctgac gcaccatttc cgccacatat
    10321 aaagcactct gatcgatttt ccgaacaaac cgctcgtaat gcagttcctc attcaacgcc
    10381 tgatcgcgct gttgcacggt aatcatcttc tcctcgagca tattgttcaa aatatacttc
    10441 tggcgcaact tggcacgttc tggattaaca atcggattat aggcagacgg agccttgggc
    10501 agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc ggacattctt attgaaatag
    10561 atttgcgcgg cagatgcaaa accataggcg cgctgaccga ggtaaatctg attgaaatac
    10621 aactcgagga ttttgtcttt gcttaaagac tgctcgattt tataggcaag caacacctca
    10681 ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa aatttttcgc cacctgctgc
    10741 gtaatcgtac tcgcacccga ctgcacgctg ccggacacga cattgccgac ggcagcgcgg
    10801 gcaacacccc aaacatccac cccccaatgc cggtaaaagc gtttatcctc ggcggcgata
    10861 accgcattcc gcaacacctc tgggaaatcg ccgatttttg taaattcgcg ccgctgctcc
    10921 ccatacatac cgatgacttc cccatccgcc gaataaatag tcaacggcat tttaggctgg
    10981 taatgctgca aagaatccaa agacggcagt ttcggatacg ttaccaaaat agcaatggca
    11041 accaaaccca ctccaaatac acaaaaccca aaaaccaaac caaaacaagt cgttaaaatc
    11101 tttttaatca tagctgaata ataatttacc attattggta ttaaataaag taaaatagca
    11161 accgatttct acaaagcacg gtttcaatgt gcaaagaaca aggaatccat tacggatacc
    11221 gaaacggtta ctcactgtac aaataaagca ggaactttca tcatgcgctt gtttaaaagc
    11281 ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa aatcttcggg actcaataac
    11341 cgcgcggcaa tcggcatcga tatcgaccag cattccatca aaatggtcca attgtcagga
    11401 cgtagtttaa accaaattca attggaaaaa tacgtcattg ccaaattacc aaagaatatc
    11461 attcaaggca ataaagtcca aaattacgat caacttgtta catatttgca acaagcctat
    11521 gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg tcccgcaaaa tctggcaacc
    11581 atcgaacaat tgacctacac agacaaagat gcagaattag acctgcaggg gttcgtggag
    11641 tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag ccaattacga ctatcaggtc
    11701 ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg catcgagaaa ggatgaaatc
    11761 gaacccctga ttgacgcatt caacgccgcc ggtatgaaat tatccgcgct tgatgtggac
    11821 attttcggac aatacaacgc ctacgcgcta tggataaacc atttcgcccc cgagcttgca
    11881 gccgaaaaag tcgccatttt cggcgtatat gccgcacaga cctacgcctt ggtcatccaa
    11941 gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg aagaacagct caaccaactc
    12001 atccagcgca cctatcaggt aacagaagaa aaagcggaag aaatcatcaa ctccccgcaa
    12061 aaaccttccg attaccaaga aagcgtggca aactatttca accagcagat tacccaagaa
    12121 atacaaaggg tcttgcagtt ttattacacc acgcagaccg cagacgatat gaccgacatc
    12181 aagcatatcc tgctgaccgg gaaagcggcg cgccaggaag gtatcgccca aaccgtcgcc
    12241 tcacaaacca atgcagatgt acaatgcgtc catcccgcgc gttattttgc ggacaacctc
    12301 aaaacagaca aacaacaatt cgaacttgat gcgccgacac tgaccagggc gttcggtttg
    12361 gcggtacggg gattataatt atgaacaatt taatcaaaat caacctcctc ccctacaggg
    12421 aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct gatgtacggt gccgtgctga
    12481 cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga caatatgatc aataaccagt
    12541 cggaaagaaa cacgctgctg gaaacctcca tcgcacactt ggataccgag ctgtcggaaa
    12601 tacaaaagct caaacaggaa aaagatgcct tcctgattaa gaaaaacaaa atcgaggagc
    12661 tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag cctgaatgag gccgtccccg
    12721 gaagcaccta cctgacctcg ctggatgccg ttaccgccga ctcttatcgg ctcagcggca
    12781 ggacatccag cgacaaccgc gttgccgcca tgatgagggc gatgcccaat accggcatat
    12841 tcaagcaacc cgaattgtta agcatcaaga aaaacaattc gcatcaagaa tttacccttc
    12901 aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa agagaatccg gcttcggaaa
    12961 acgcacagga ggcaaactga atggcttcta aatcatctaa aaccaacttg gatctcaaca
    13021 accttcacct gctcaacctt cctgccaggc tttttatcgc cctgctggcc gttgccgccg
    13081 tgctggggct cggttatgcc ggattgttca aaagccagat ggaatccctt gaggaatacg
    13141 aagcaaaaga aaccgaactg aaaaacacct acaaacagaa aagtatcgac gcggccagcc
    13201 tgaacaacct gagggacgaa cttgcctcaa tccgctctgc cttcgatatc atgttgaaac
    13261 agctgccgac agatgcagaa attcccaacc tggttcaaga gcttcatcag gcaggttcga
    13321 gcaacggcct gcgcttggac agcgttatgc cccaacctcc cgtagatgac ggccccatca
    13381 aaagattacc ctattccatt tccattaccg gaaattacga acagatcagc caatttaccc
    13441 gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc gctgaaaatc gcccaatctc
    13501 cggaaaacgg cggcaatcct gacggcaaga gcagcatcct gaacctcagt gccattgcca
    13561 ccacctacca agcaaaatcc gtagaagagc ttgccgcaga agcggcacaa aatgccgagc
    13621 aaaaataact tacgttaggg aaaccatgaa acactatgcc ttactcatca gctttctggc
    13681 tctctccgcg tgttcccaag gttctgagga cctaaacgaa tggatggcac aaacgcgacg
    13741 cgaagccaaa gcagaaatca tacctttcca agcacctacc ctgccggttg cgccggtata
    13801 cagcccgccg cagcttacag ggccgaacgc attcgacttc cgccgcatgg aaaccgacaa
    13861 aaaaggggaa aatgcccccg acaccaagcg tattaaagaa acgctggaaa aattcagttt
    13921 ggaaaatatg cgttatgtcg gcattttgaa gtctggacag aaagtctccg gcttcatcga
    13981 ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat ttgggacaaa actacggtag
    14041 aatcgaaagc attaccgacg acagcatcgt cctgaacgag ctgatagaag acagcacggg
    14101 caactgggtt tcccgtaaag cagaactgct gttgaattct tccgacaaaa acaccgaaca
    14161 agcggcagca cctgccgcag aacaaaatta agaagaggat tactccatta tgaataccaa
    14221 actgacaaaa atcatttccg gtctctttgt cgcaaccgcc gcctttcaga cagcatcggc
    14281 aggaaacatt acagacatca aagtttcctc cctgcccaac aaacagaaaa tcgtcaaagt
    14341 cagctttgac aaagagattg tcaacccgac cggcttcgta acctcctcac cggcccgcat
    14401 cgccttggac tttgaacaaa ccggcatttc catggatcaa caggtactcg aatatgccga
    14461 tcccctgttg agcaaaatca gtgccgcaca aaacagcagc cgtgcgcgtc tggttctgaa
    14521 tctgaacaaa ccgggccaat acaataccga agtacgcggg aacaaagttt ggatattcat
    14581 taacgaatcg gacgataccg tgtccgcccc cgcacgcccc gccgtaaaag ccgcgcctgc
    14641 cgcaccggca aaacaacagg ctaccgcacc gtctaccaag tccgcagtat ccgtatccaa
    14701 accctttacc ccggcaaaac aacaggctgc cgcaccgttt accgagtccg tagtatccgt
    14761 atccgcaccg ttcagcccgg caaaacaaca ggcggcggca tcagcaaaac aacagacggc
    14821 agcaccagca aaacaacagg cggcagcacc agcaaaacaa accaatatcg atttccgcaa
    14881 agacggcaaa aatgccggca ttatcgaatt ggctgcattg ggctttgccg ggcagcccga
    14941 catcagccaa cagcacgacc acatcatcgt tacgctgaaa aaccataccc tgccgaccac
    15001 gctccaacgc agtttggatg tggcagactt taaaacaccg gttcaaaagg ttacgctgaa
    15061 acgcctcaat aacgacaccc agctgattat cacaacagcc ggcaactggg aactcgtcaa
    15121 caaatccgcc gcgcccggat actttacctt ccaagtcctg ccgaaaaaac aaaacctcga
    15181 gtcaggcggc gtgaacaatg cgcccaaaac cttcacaggc cggaaaatct cccttgactt
    15241 ccaagatgtc gaaatccgca ccatcctgca gattttggca aaagaatccg ggatgaacat
    15301 tgttgccagc gactccgtca acggcaaaat gaccctctcc ctcaaagacg taccttggga
    15361 tcaggctttg gatttggtta tgcaggcacg caacctcgat atgcgccagc aagggaacat
    15421 cgtcaacatc gcgccccgcg acgagctgct tgccaaagac aaagccttct tacaggcgga
    15481 aaaagacatt gccgatctgg gcgcgctgta ttcacaaaac ttccaattga aatacaaaaa
    15541 tgtggaagaa ttccgcagca tcctgcgttt ggacaatgcc gacacgaccg gaaaccgcaa
    15601 tacgcttgtc agcggcaggg gcagcgtgct gatcgatccc gccaccaata ccctgattgt
    15661 taccgatacc cgcagcgtca tcgaaaaatt ccgcaaactg attgacgaat tggacgtacc
    15721 cgcgcaacaa gtgatgattg aggcgcgtat cgtcgaagcg gcagacggct tctcgcgcga
    15781 tttgggcgtt aaattcggcg cgacaggcaa gaaaaagctg aaaaatgata caagcgcatt
    15841 cggctggggg gtaaactccg gcttcggcgg cgacgataaa tggggggccg aaaccaaaat
    15901 caacctgccg attaccgctg ccgcaaacag catttcgctg gtgcgcgcga tttcctccgg
    15961 tgccttgaat ttggaattgt ccgcatccga atcgctttca aaaaccaaaa cgcttgccaa
    16021 tccgcgcgtg ctgacccaaa accgcaaaga ggccaaaatc gaatctggtt acgaaattcc
    16081 tttcaccgta acctcaatcg cgaacggcgg cagcagcacg aacacggaac tcaaaaaagc
    16141 cgtcttgggg ctgaccgtta cgccgaacat cacgcccgac ggccaaatca ttatgaccgt
    16201 caaaatcaac aaggactcgc ctgcgcaatg tgcctccggt aatcagacga ttctgtgtat
    16261 ttcgaccaaa aacctgaata cgcaggctat ggttgaaaac ggcggcacat tgattgtcgg
    16321 cggtatttat gaagaagaca acggtaatac gctgaccaaa gtccccctgt tgggcgacat
    16381 ccccgttatc ggcaacctct ttaaaacacg cgggaaaaaa accgaccgcc gcgaactgct
    16441 gattttcatt accccgagga ttatgggtac ggccggcaac agcctgcgct attgatgcgt
    16501 caaaataagg gcatatgttt tacggcatat gccctttctt tatgcttttt gccgcgaccg
    16561 aaatgccgtc attcccgcgc aggcgggaat ccagtccgtt cagtttcggt catttccgat
    16621 aaattcctgt tgcttttcat ttctagattc ccacttttgt gggaatgacg gcggaaggga
    16681 taaatcctcg caatccaaag cctcgtcatt tccacaaaaa acagcaaccc gaaacacccc
    16741 gtcattcccg cgcaggcggg aatctaggtc tttaaacttc ggttttttcc gataaattct
    16801 tgccgtattg aaattctaga ttcccgcttt ctcgggaatg acgggatatg ggtttccgtg
    16861 cggacgtgtt cggatttccg cctgcgcggg aatgacggcg acagatgccc aacggtcttt
    16921 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
    16981 gaaccgattc acttggtgtt tcagcacctt agagaatcgt tctctttgag ccaaggcgag
    17041 gcaacgctgt actggttttt gttaatccac tatagtattg ataaacatat tatcttcaat
    17101 atattcaatt ggataattgt ttacctaagc aaagataatt gccttttcct gacaaataag
    17161 tgaaatcaac ggattgtcaa aacacagcct gaaataaaaa acctccctga tttcttttat
    17221 ttttccttaa aatcagaaag gttcgggatg gtcgggttat ttttccaaac gtaccgccgc
    17281 cctgccgatt tcgtataaaa ttccgccgta acccgacaag cccgaaccct gtcgccccga
    17341 aaggcggggt gtcaaacatt aaggaattgt gatgaaaaac tttaacggca aactcatcct
    17401 catcggactg atgggcgcgg gcaaaaccac gctgggccgg caaatggcgc agcggctgga
    17461 ttaccgtttt tacgacagcg atcacgaaat cgccgcagcc gccggcgttc ccatccccac
    17521 catatttgaa atggaaggcg aacagggatt ccgttcgcgc gaaaccgcca tactcaaaaa
    17581 gctggttatc ctgccccata tcgtcctgtc caccggcggc ggcgcggtgt taaaagaaga
    17641 aaaccgcgcc cttatccgca aaagcggcac ggtcgtctat ctgcacgccc cgcccgaaac
    17701 cctgctcgaa cgcacgcgct gcgacaacag ccgtcctttg ctgcaagttg ccgatccttt
    17761 ggcgaaatta cgtgaactct acgccgcacg cgaccccgtt taccgccaaa ccgccgactt
    17821 taccgtagaa tcggcaaact gccgggaaac cgtgcaaacc ctgctcaaac gcttatcccg
    17881 ataaaccggc atatgcgccg cgcccagaaa accaagccgc gccccgcccg gcgggccggc
    17941 ggttcaaact ttaaggaaca acaatgaaaa cactgaccgt acacaccccg tcccacagct
    18001 accccatctt tatcggcaac ggactgctgc cgcaggcagg aagcctgctc aaaccgcatt
    18061 tgggcaaacg cgccgccatc atcgccaacg aaaccgtcgc cccgctctac ctcggcacgc
    18121 ttcagacggc attggatgcg gcaggcgtat cccatttcag catcatcctg cccgacggcg
    18181 aggcgcacaa aaactggcag acgctcaacc tcatctttga cgggctgatg caaaaccgcg
    18241 ccgaacgcaa aaccacatta atcgcactgg gcggcggcgt gatcggcgac atggtcggct
    18301 ttgccgctgc cacctaccag cgcggcgcac cgttcgttca aataccgacc acactgttga
    18361 gtcaagtcga ctcatcggtg ggcggcaaaa ccgccatcaa ccacccgctc ggcaaaaata
    18421 tgatcggcgc attctaccag ccgcaggcgg tgcttgccga cttggacacg ctgcacaccc
    18481 tgcccgtccg cgaattgtcc gccggtatgg cggaagtcat caaatacggc gcgctcggcg
    18541 acatcggttt tttcgaatgg ctcgaacaaa acatatccgg actgatgacg ctcgatcggg
    18601 aaaaactcgc ccaagccgtg taccgctgct gccaaatgaa ggcagacatc gtcgcccaag
    18661 acgaaaccga acagggcata cgcgcatggc tcaacctcgg acacaccttc ggacacgcca
    18721 ttgaaaccga gatgggttac ggcacttggc tgcatggaga agccatcgcc gccggctgcg
    18781 tgttggcggc gcgtttgtcc gaacaactgg gcaaaacctc cgccgcagat accgcgcggc
    18841 tcgccgccct gctcgaagcc gctggactgc cgtccgcacc gcccgtgttt gcctttgaaa
    18901 aatggctcga acatatgagc cacgataaaa aagtcagcgg cggcatcatg cgctttatcg
    18961 gtctgaaccg gctgggcgaa gccaacatca ccgaaattac cgacacggac atcctccgcc
    19021 gcaccctgca accgtatctc tgatttcctc tgccgatgtg ctgccgcgcg ggtttgacgc
    19081 acgatgatga ttttccatca tctttctccg taaaagcggg aatccggtcc gttcggtttc
    19141 ggtcgtttcc gataagttcc cgttgctttt catttctaga ttcccacttt cgcaggaatg
    19201 acggtggaaa ggtttttgtt gtttcggaga agtttctgca accctagaat ctcgttattt
    19261 ccacaaaaaa cagaaaccca aaacagcaac ttaaaacctc gtcattcccg caaaagcggg
    19321 aatccggtcc gttcggtttc ggtcgtttcc gataaattcc cg
//
LOCUS       DAWGJV010000024        19108 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWGJV010000024 DAWGJV010000000
VERSION     DAWGJV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.024"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            209..1273
                     /locus_tag="WHQ94_000594"
     CDS             209..1273
                     /locus_tag="WHQ94_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ2127923.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WHQ94_000595"
     CDS             1414..1737
                     /locus_tag="WHQ94_000595"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ2127924.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WHQ94_000596"
     CDS             1809..2132
                     /locus_tag="WHQ94_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ2127925.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WHQ94_000597"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WHQ94_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ2127926.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WHQ94_000598"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WHQ94_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ2127927.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSDTSAEILGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WHQ94_000599"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WHQ94_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ2127928.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WHQ94_000600"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WHQ94_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ2127929.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGSIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WHQ94_000601"
     CDS             4530..4883
                     /locus_tag="WHQ94_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ2127930.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WHQ94_000602"
     CDS             4873..6687
                     /locus_tag="WHQ94_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ2127931.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WHQ94_000603"
     CDS             6877..7554
                     /locus_tag="WHQ94_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ2127932.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WHQ94_000604"
     CDS             7568..8974
                     /locus_tag="WHQ94_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ2127933.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WHQ94_000605"
     CDS             9092..11518
                     /locus_tag="WHQ94_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ2127934.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WHQ94_000606"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WHQ94_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ2127935.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WHQ94_000607"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WHQ94_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ2127936.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHEQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WHQ94_000608"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WHQ94_000608"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ2127937.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WHQ94_000609"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WHQ94_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ2127938.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13483..13788
                     /locus_tag="WHQ94_000610"
     CDS             13483..13788
                     /locus_tag="WHQ94_000610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127939.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPRSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHIIITALPLSLSKPQIIMTPSLLLSGLTFRLVLALIAVSLLWGVYFWAVSA"
     gene            13785..14510
                     /locus_tag="WHQ94_000611"
     CDS             13785..14510
                     /locus_tag="WHQ94_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ2127940.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14534..15409
                     /locus_tag="WHQ94_000612"
     CDS             14534..15409
                     /locus_tag="WHQ94_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ2127941.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVIRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15486..16400
                     /locus_tag="WHQ94_000613"
     CDS             15486..16400
                     /locus_tag="WHQ94_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ2127942.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILSDVAKQIGGERVS
                     IQNLVGANQDTHAYHMTSGDIKKIRSAKLVLINGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            complement(16680..19097)
                     /locus_tag="WHQ94_000614"
     CDS             complement(16680..19097)
                     /locus_tag="WHQ94_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2127943.1"
                     /translation="METVAAKGFSGSLFDHNGNGIRTATGWVSADDGLLVRDLNGNGI
                     IDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVWQDLNQDG
                     ISQTNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAA
                     DNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQ
                     LALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSD
                     KAKATIDAARDRIAVLDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLL
                     FQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRS
                     WYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKN
                     VSLYGNDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYDYATGRKDIIRFTD
                     GITADMLTFTREGNHLLIKAKDGSGQVTVQSYFQNDGSGAYRIDEIHFDNGKVLDVAT
                     VKKLVQQSTDGSDRLYAYQSGSTLNGGLGDDYLYGADGNDLLNGDAGNDSIYSGNGND
                     TLNGGEGNDALYGYNGNDALNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSGSDTYVF
                     GEGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFYG
                     ENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANTQSVQQP
                     LLVTPSA"
BASE COUNT         4951 a         5912 c         4217 g         4028 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac tgaggaaggc caagcaggaa
     2581 ttcgaagctg ccgccgctca ggttcgagac agcctcaaag aaatcggtac ggatatggag
     2641 ggtaatctgc acgacatttc caatggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgccg atttcggtgt cgatgaaaac ggcaatcccc ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tccgaacgtt ccgatacttc cgccgaaatc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgc
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg atactgctgt tgaaacgcct gttccgcaca ccacttccct gcgcaaacag
     3001 gcaataagcc gcaaacgcgg tttgcgtccg aaacaccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtcgag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcctaa tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccgtt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tctccttcat cttggggatg tttgtcgcat tcggtacaac
     3601 gtttgaagtc cccattgtcg ttatcctgtt aaccaaaatt ggtgtggtaa caaccgaaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacagcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctatcaggtc gtcaagctgc ctgccaacat cgacgaaacg gtcagacaga
     4021 acgaagaccc tgcccgttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattaggca aaccccactc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtc caaagcggcg aaccgatgga caaagccggt gcctacgccg
     4381 tacaaggcat aggcagcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccaccct
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttgcct
     4561 ctttcagcct gatcgcactg ataatcctgt ccctttcctg ggaactgtgg attgcaccgt
     4621 tgcgcccggg cggctcgtgg ctcgcgctca aagccctccc cctctgtctg ccgctttcag
     4681 gcatcttgaa aaagaaaatc tatacttacc aatacagctc catgctggtt ctgatttact
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatcccgc agaacagatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgacc gtatccggcg cgcaccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aaactgaaac cgtcgcccga cttttaccac gatgccgccg ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaaactg tttgatgtca aaatccccgc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca cttcggacaa gaacgcatcg tgaccctgtt tgcctggtcg
     5281 ctgcttatcg gctccctgct tcaatcctgc atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgccgc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaactc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cggcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 actggcatcc gctacgaaac tgccgtcgaa cgcgtcgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 tacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctta aacgccccct gacccccgca tcgcttttcc taatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccctttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa atcactaaaa taccgccccc actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcaccgc tgctacccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggt
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atgcggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtaccccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat cgtcgtcgga ctgctgatgg cgtacatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgca tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggcgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc caccacgttt cccacgaaat gcgctccccg cttgcccgta tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacgggct gcggatgcgc ttcatcctac ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa tgccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc ccggctactt
     9121 cttcatgccc aaccacatta tcctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagccg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgtctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacatccg ccgattggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgacctcgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 acatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcaaccg tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag ccctcgaagc cgccgacccc gccctaatcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgtc caattccaat tcggcagcca
    10501 ctccctctat ggcgacatcc tgaccgcacg ttgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac catcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcatacccca aaacactgtc
    10981 cctaagaaac aacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaaccgcaac gaatgcgaat ttggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcgcacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccccttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa tgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatt cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc gtcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacagcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcgcggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gagcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt acccgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcctccc aagctgcaaa agtcatctac ctcagccccc
    12601 aagggaaacc gctgacacac caaaaagcgg tagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acggtatgag ggcatagacg aaaggcttct gcaaagcagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcagtttaa
    13021 tcccaaagga atcccgcatc ttgaataaaa tcctacaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggtc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgcc ccgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatct acctttctta
    13921 aatccttgat gggattgcag cccatcgaca ctggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcagt ccgacatcga ccgctcgcag cccatgaccg
    14041 ttttcgactt ggcggcaatg gggctgtggt atgaaatcgg ctttttcaaa gggataaata
    14101 ccgctcaaaa acaacgcgtt cacgaagcct tggagcgtgt aggaatgcgg cagtttgccc
    14161 gccgccaaat cgcccacctc tcaaacggac aattccaacg cgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctag acgaaccttt caacgctgtt gatgcacgga
    14281 caacctacga acttctcgac gtattgcaga aatgccattg cggcggacac gctatcattg
    14341 ccgttttgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcgtcataa atgccgtctg
    14521 aaaccgaaaa accatgaatc tctacgacct gctccttgcc ccctttgcag aattcgactt
    14581 tatgcgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac ccgtcggcgt
    14641 attcctcgtc atacgccgta tgagcctgat aggcgacgca ttgagccacg ccgtcttgcc
    14701 cggtgctgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg gtttgggcgg
    14761 cgtagccgca ggtatgctga tggcactgct tgccggactc gtcagccgct tcaccaccct
    14821 gaaagaagat gccaactttg ccgcctttta tctcagcagc ctcgccatcg gtgtagtcct
    14881 cgtcagcaaa aacggcagca gcgtcgattt gctgcatctg cttttcggct ctgtacttgc
    14941 cgtcgatatt cctgccctgc aactcatcgc cgccgtatcc accctcacac tgcttaccct
    15001 tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgttcc tcaaatccgt
    15061 cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca tgaacctcgt
    15121 atccggcttt caagccctcg gaacactcat gtccgtcgga ctcatgatgc tgccagccat
    15181 taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg ttctgacagc
    15241 cctgctgtgc ggcttgagcg gactgcttat ttcctaccac atcgaaattc cctccggtcc
    15301 cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg gcaaagaagg
    15361 cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac cccgcaagcc
    15421 gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat cacaggagaa
    15481 cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc gccgcaactg
    15541 ccgcccccct tccggttgta accagtttca gcattttgag cgacgtagcc aaacaaatcg
    15601 gcggagagcg cgtatccata caaaatttag tcggcgccaa ccaagatacg cacgcctatc
    15661 atatgaccag cggcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg attaacggct
    15721 tgggacttga agctgccgat gtgcaacgcg ccgtcaaaca aagcaaagta tcctataccg
    15781 aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac catcacgacc
    15841 acgatcatga ccacgaagga caccaccacg accacggcga atatgacccc cacgtctgga
    15901 acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg ataaaggccg
    15961 accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg cagctcaaaa
    16021 aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa cgcaaagtcc
    16081 tgaccgggca cgatgccttt tcctatatgg gcaaacgtta ccatatcgaa ttcatcgccc
    16141 cgcaaggcgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc atcatccgac
    16201 aaatcaaacg cgaaggcatc aaagccgtat ttaccgaaaa tatcaaagac acccgcatgg
    16261 ttgaccgcat cgccaaagaa accggcgtca acgtcagcgg caaactgtat tccgacgcac
    16321 tcggcaacgc gcccgcagac acctacatcg gcatgtaccg ccacaacgtc aaagccttga
    16381 ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg caaaaggccg
    16441 tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
    16501 agatagtacg gtaaggcgag gcaacgctgt actggttttt gtgaatccac tatattgcta
    16561 gaagtgcacc gattaaggtt aattaaaata cgacccttat cccaatatta agaaaaacag
    16621 caatcaaaat aaacttgatt gctgtttttc agtttaagcc atgaatgtga ttaggctcct
    16681 tatgcagatg gcgttaccaa taacggctgc tgtacggatt gtgtattggc atccacattt
    16741 cctcctgtcg cagcagtatt agaaccgaac acagacatag actgtaccaa attattgcca
    16801 gcattaatat acttggcaaa atctggatta ctgatagctg catcatcaaa gacaaatgta
    16861 tctacacgat ggttttcacc atagaagaat ccggaaatac gtacgttgtc ttgttcagaa
    16921 gcgctaagca ccaaatcact tccggaacgg ataaaatgaa catctgctgc tttaaatcct
    16981 ttaaagtgca tagtgtcaga gtttttatcc acatggtaat tatagaccgt atcctgaccg
    17041 aagccttcgc cgaagacata agtatccgaa ccgctgccgc cctccaagta atcattaccg
    17101 gcaccgccga tcagagtgtc gttaccgtct tcgccgttca aatgatcatt gccttcgcca
    17161 ccattcagtg catcgttacc attatagccg tacagggcgt cgttgccttc tcctccattg
    17221 agcgtatcat tgccattgcc actgtagata ctgtcgttgc ctgcatcacc attcagcagg
    17281 tcattcccgt cggcaccgta cagatagtca tcgcccaatc cgccatttaa ggtacttccg
    17341 gattggtagg catacaatct gtccgaaccg tcggtggatt gctgtaccag ttttttgaca
    17401 gtggcaacat ccagtacttt gccgttatcg aaatgaatct catcgatacg gtaagcacct
    17461 gagccatcgt tctggaaata ggactgaaca gtcacttgtc cactgccgtc ttttgccttg
    17521 ataagaagat ggttgccctc tcgggtaaaa gtcagcatat cggctgtaat accgtcggta
    17581 aagcggatga tgtctttgcg tccggtagcg tagtcgtaat tatagaccgt atcctgaccg
    17641 aagcctttgc cgaagacata agtatccgaa ccgctgccgc cctccaagta atcattgcct
    17701 gcaccgccga tcagagtgtc gttgccgtca ttaccatata aagaaacatt tttattatga
    17761 ccaaagccta cattttgcag gatatcatct gcttgcgtac ccgatgtttt agctaataat
    17821 gcaacggtct cctgacccaa cactttctgg taatcttcaa atttacctgc tttttttgcc
    17881 tcctccacat aatcggccat tagtcttcgg ccttcatacc aagaacgaag ttcgccatat
    17941 gcaagcatct cggccaaatc cacaaaagct ttttgcggat tagtttcttt gacatggtta
    18001 aatgcttgaa caagaccact aaaatccaaa gtgaacgtat cattttccat tttgaaactg
    18061 atttgattca aatatggctg caaacgggtt tggaataaca ggttttggta gatgtttttg
    18121 gcgagatggt cgtatgtatc gttggttact ttgacgatat taagcgcatc ttcctcgctc
    18181 atgtaataga gtgtgttgga atcctgcccc gtgtaggcat caagcacggc aatgcggtcg
    18241 cgggcggcgt caatagttgc tttagcttta tcagaaaggg aaactaaagc gttctttttt
    18301 agttgtgcta cttgggatgg tgtcagtgca ataccttcat tagccgtttg cgtccaatcg
    18361 gttgaaagtc gcattggcga ttttttgccc cagttcgaat cggtttccgc ccatttgtga
    18421 atcaaattat ctaacaatgc caactgtgct tctttagttt cggcggcaga ataagctttc
    18481 agcatattgg ccaaatcgcc ggacaatgcg gcagcttcgc gcaaatcgcg cagacgacca
    18541 atgcccgcaa gattggcggc ttttgcctgt tcggcagtga gttccacttt gtctttgaag
    18601 cggctgtgca ggttgtcggc tgctaaaagt aaatccccca tttttgcggt tgtaccgttt
    18661 gttttggtgt agctgccttg ctgagccaaa gtgttaccgt taccgagatt tttatttaca
    18721 tctttatagg cgagatccaa agattgaata cccaattctt caagggtgcg gagttcgttg
    18781 gtttgggaaa tgccgtcctg gttgagatcc tgccatacac gcagggattg gaatgcggcg
    18841 tctgccgcgt tgatgatgtt gtcgccgttt gaatccaatt cggccaaagc tgcgtagccg
    18901 tgtttggcaa aagaaccgtc tgccagtttg gtgttgtcgc cgaagagttc cgcgccgttg
    18961 tcgatgatgc cgttgccgtt caaatcgcgg acgagtaaac cgtcatcggc agaaacccag
    19021 ccagtggcgg tgcggatgcc gttgccgtta tggtcgaaga ggctgcctga aaagcctttg
    19081 gcggcgactg tttctatgcc gtcgccgt
//
LOCUS       DAWGJV010000025        18865 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWGJV010000025 DAWGJV010000000
VERSION     DAWGJV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.025"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..79)
                     /locus_tag="WHQ94_000615"
     CDS             complement(<1..79)
                     /locus_tag="WHQ94_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ2127944.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WHQ94_000616"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WHQ94_000616"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ2127945.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WHQ94_000617"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WHQ94_000617"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ2127946.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVVDMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WHQ94_000618"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WHQ94_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ2127947.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     AADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WHQ94_000619"
     CDS             4804..5868
                     /locus_tag="WHQ94_000619"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ2127948.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5888..6847
                     /locus_tag="WHQ94_000620"
     CDS             5888..6847
                     /locus_tag="WHQ94_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ2127949.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPSGKAEYHG
                     KAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            6986..7663
                     /gene="tsaB"
                     /locus_tag="WHQ94_000621"
     CDS             6986..7663
                     /gene="tsaB"
                     /locus_tag="WHQ94_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ2127950.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRTDGETHLFHQEAGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSGYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRALL"
     gene            7660..8100
                     /gene="rimI"
                     /locus_tag="WHQ94_000622"
     CDS             7660..8100
                     /gene="rimI"
                     /locus_tag="WHQ94_000622"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ2127951.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQHQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8094..8831
                     /locus_tag="WHQ94_000623"
     CDS             8094..8831
                     /locus_tag="WHQ94_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ2127952.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAALIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAIYGQLFHGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVNPERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8893..9534
                     /gene="pyrE"
                     /locus_tag="WHQ94_000624"
     CDS             8893..9534
                     /gene="pyrE"
                     /locus_tag="WHQ94_000624"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ2127953.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9608..10042
                     /locus_tag="WHQ94_000625"
     CDS             9608..10042
                     /locus_tag="WHQ94_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127954.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10039..11349
                     /gene="argA"
                     /locus_tag="WHQ94_000626"
     CDS             10039..11349
                     /gene="argA"
                     /locus_tag="WHQ94_000626"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ2127955.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDHHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGSETQRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPEARRKAYRNNGRNSHILVRRLHR"
     gene            11445..13460
                     /locus_tag="WHQ94_000627"
     CDS             11445..13460
                     /locus_tag="WHQ94_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ2127956.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDAAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSESEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPRHIGLQGGSNGGLITAAAFVCEPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLHETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(13574..14530)
                     /locus_tag="WHQ94_000628"
     CDS             complement(13574..14530)
                     /locus_tag="WHQ94_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2127957.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14766..15731
                     /locus_tag="WHQ94_000629"
     CDS             14766..15731
                     /locus_tag="WHQ94_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2127958.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            complement(16038..16190)
                     /locus_tag="WHQ94_000630"
     CDS             complement(16038..16190)
                     /locus_tag="WHQ94_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127959.1"
                     /translation="MRFVVLASFSGRSCNESVIDFDVSLRLTALSLTLSHRERGRVAV
                     ASLFAA"
     gene            16221..16544
                     /locus_tag="WHQ94_000631"
     CDS             16221..16544
                     /locus_tag="WHQ94_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ2127960.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            16658..18836
                     /locus_tag="WHQ94_000632"
                     /pseudo
     CDS             16658..18836
                     /locus_tag="WHQ94_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT         4242 a         5874 c         5109 g         3640 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctacctccc aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtagttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccga agctggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacttgca cgacgacacg ccgcatgaag aaaaagtgcg ccgcctcgaa gctttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggttga aggtatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgatcaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaattgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgcag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcgacga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggatgtgg aagaatgccc tacgccgaac gcgtcgtatt ccgactcgct gcgttttggg
     3301 aagccagcta aaccgccgta ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccatacca gcttgtcttc gggcgtatcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccggtcagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagc tcgaacagag cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggccgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gccttcagct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc actgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgaaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaactggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gttccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgataccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaaacc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagca aacctgtgaa ccgaactgcc ttctgctgcc tttctttgac
     6061 cgccgccctg attctgaccg cctgcagcag cggaggcggc ggtgtcgccg ccgacatcgg
     6121 cgcggggctt gccgatgcac taaccgcacc gctcgaccat aaagacaaaa gtttgcagtc
     6181 tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct caatacgggc aaattgaaga acgacaaggt
     6301 cagccgcttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caaatataca aacaggacca ctccgccgtc gttgccctac agattgaaaa
     6421 aatcaacaac cccgacaaaa tcgacagcct gataaaccaa cgctccttcc ttgtcagcgg
     6481 tttgggtgga gaacataccg ccttcaacca actgcccagc ggcaaagccg agtatcacgg
     6541 caaagcattc agctccgacg atgctggcgg aaaactgacc tataccatag atttcgccgc
     6601 caaacaggga cacggcaaaa tcgaacactt gaaaacaccc gagcaaaatg tcgagcttgc
     6661 ctccgccgaa ctcaaagcag atgaaaaatc acacgccgtc attttgggcg acacgcgcta
     6721 cggcggcgaa gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg cccaagaaat
     6781 cgccggctcg gcaaccgtga agataaggga aaaggttcac gaaatcggca tcgccggcaa
     6841 acagtagcat ttgaaaaatg ccgtccgaac acgatagttt accgttcgga cggcattttt
     6901 gcgccatact gccctaggac acgacggcac ggcaggcaag ctgatttcac tatataatgc
     6961 cgtctgaacc aacgagaacc ataccatgca agctgatttt aaccgtcccg tcctggccat
     7021 cgataccggt acttcctatt tgtcgctcgc gctgcgcacc gacggcgaaa cccacctgtt
     7081 ccatcaagag gccggcagcc gccaatccga actgatcctg ccggaaatcc gcacgctgtt
     7141 tcacgatgcg ggcattaccg ccgccgattt gggggcgatt gtttatgcga aaggccccgg
     7201 cgcgtttacc ggactgcgta tcggtatcgg cgtggcgcaa ggtttggcaa cgccgtttga
     7261 tacgccttta atcggcatcc ccacgctcga tgccgccgcc tcgctgccgc cgccgcaaag
     7321 ctgcatactc gccgctgcgg acgcgcgtat gggcgaagtg ttttatgcgt ggttcgacac
     7381 gctgaaccgc cgccgtttga gcggttatca agtcggtagg gcggcagaca tccggctgcc
     7441 ggagggatgt gccttttcag acggcatcgg caatgcgttc gcgctggaaa accgtccgcc
     7501 cttctcaggc aaacccgata tgccgactgc cgccgatttt ctcaatctgg cggaagaagg
     7561 cggttttccc gccgtcgctg cggcacacgc cgagctgctc tacgtccgca acaaaatcgc
     7621 cttgactgcc aaagaacagg cggaacggag ggcgctcctg tgaacatccg ccgcgccgtt
     7681 tatgccgatt gcgcggcact cgccgcactc gatgccgtct gcaacccgtc cgcgtggacg
     7741 caacaccaat ttgagtccgc attggtttcg ccgtccgaac aggttttcct tgcggaaaaa
     7801 gacggcaggt tggccgcctt tatcgtttgg cagaacctgc ccgacgaatc cgaactgcac
     7861 ctgattgcca ccgcgcccga atgccgccgc cggggtgttg cgtccgccct gctcgaatat
     7921 tggtacgcac acctgcccga aggcacgcaa cgcctgctgc tcgaagtccg cgcaggcaac
     7981 accgccgcac aggcactgta cgcggcgcac ggcttcagca ttgcgggcag gcggaaaaac
     8041 tattaccgta cagccgacgg taaaaccgaa gatgccgtct tgatggagaa aatatgttaa
     8101 gcgcgcgcta cctccacctg cacgaagctt tgggtttagg cccaatgtgg ctgaaacagg
     8161 ccgccgccgt cctgccgccc aaaaacacac ccgcaacccc ggcacaggca cgtccccaaa
     8221 ccgtccgcgc cgccctgatc cgcccttccc aaccccataa cggtcaggcg cggctcgaaa
     8281 cgatgaaagc gttggaaacc gccgccgtac ctacgcgcaa acccgcgcct gaaaccgaaa
     8341 cgcctctgcc cggcctttca gacggcatcg cccccgttcc cgccgcttcg ggcatcacca
     8401 agcttgccgt cgtcagcctt tgcccaccga tcgaggatgc gatttacggg caactgttcc
     8461 acggcaaagc aggtgttctg cttgacaaca tactcaaagc cgtaggactg gatgccgctt
     8521 atgtccacaa aacctgttgg gtgaaaaccg ccgctgtcgg caacccgatg ccgtctgaag
     8581 cggcaatcga aaccgcgacg gtttccgtca tgcaggaact cgacggctgc cgcgcccccg
     8641 ccgtcctatt cctcggacag gcgtttgtca acccggaacg gcaggcgatg attgaaactt
     8701 tgtgcggcag ccgtcccttc ttcatcatcg accatcccgc ccggctttta cgccaacccg
     8761 aactcaaagc ccgcgcctgg caggtgttga aacagttgaa acgcgccttg cggcaaggcg
     8821 gcggcagttg aagcgcaccg cacggggcgg tagaatcgca actgcgtccc aatatctgac
     8881 agaaagcaca aaatgaccga tttccgccaa gatttcctta aattctccct cgcccaaaat
     8941 gttttgaaat tcggcgaatt caccaccaaa gccggacggc ggtcgcccta tttcttcaat
     9001 gccggtctct ttaacgacgg cttgtccacg ctgcaactgg caaaatttta cgcacaatcc
     9061 atcattgaaa gcggcatccg attcgatatg ctgttcggtc ccgcctacaa aggcattatt
     9121 ttggcggcgg caaccgcgat gatgctggcg gaaaaaggcg tgaacgtccc gtttgcctac
     9181 aaccgcaaag aagccaaaga ccacggcgaa ggcggcgtgt tggtcggcgc gccgcttaaa
     9241 gggcgcgtgc tgattatcga cgacgtgatt tccgccggca catccgtacg cgaatcgatc
     9301 aaattgattg aagcggaggg tgcaaccccc gccggtgtcg ccatcgcgct cgaccgcatg
     9361 gaaaaaggca cgggtgaatt gagcgcggtt caggaagtgg aaaaacaata cggcctgccc
     9421 gtcgccccca tcgccagcct gaacgatttg tttatcctgt tgcaaaacaa tcccgaattc
     9481 ggacagttcc tcgaacccgt ccgagcctac cgtcggcagt acggcgtaga ataaaaacaa
     9541 agcatatgcc gtctgaaccg ccttacgcct cagacggcat caaacctgac acacacgagg
     9601 aaataccatg cccgcctgtt tctgccccca ctgcaaaacc cgtctctggg tcaaagaaac
     9661 ccaactcaat gtcgcccaag gcttcgtcgt ctgccaaaaa tgcgaaggac tgtttaaagc
     9721 caaagaccat ctggcaagca cgaaagaacc catattcaac gatttgcccg aggctgtttc
     9781 ggatgtcaaa ctcgttcacc gtatcggcac gcgcgccatc ggcaagaaac agatttcccg
     9841 tgacgaaatc gccggcatcc tcaacggcgg cacaacccag cccgatattc cgcccgcaac
     9901 cgccgccacc cctgctgccg caccgcaggt taccgtaccg cccgccgcgc ccgcccgtca
     9961 ggatgggttc aactggacga ttgcaaccct gtttgccctt atcgtcctca ttatgcagct
    10021 ttcctacctc gtcatcctat gagcgcgccc gacctctttg tcgcccactt ccgcgaagcc
    10081 gccccctaca tccgccaaat gcgcggcaaa acgctggtcg ccggcataga cgaccgcctg
    10141 ctcgaaggtg ataccttaaa caagctcgcc gccgacatcg ggcttttgtc gcaactgggc
    10201 atcaggctcg tcctcatcca cggcgcgcgc cacttcctcg accaccacgc cgccgcgcaa
    10261 ggccgcacgc cgcattattg caggggcttg cgcgttaccg acgaaacctc gctcgaacag
    10321 gcgcagcagt ttgccggcac cgtccgcagc cgttttgaag ccgcattgtg cggcagcgtg
    10381 tccgggttcg cgcgcgcgcc ttccgtcccg ctcgtatcgg gcaacttcct gaccgcccgt
    10441 ccgataggtg tgattgacgg aaccgatatg gaatacgcgg gcgttatccg caaaaccgac
    10501 accgccgccc tccgtttcca actcgacgcg ggcaatatcg tctggctgcc gccgctcgga
    10561 cattcctaca gcggcaagac cttctatctc gatatgcttc aaaccgccgc ctccgccgcc
    10621 gtctcgcttc aggccgaaaa actcgtttac ctgacccttt cagacggcat tgcccgcccc
    10681 gacggcacac tcgccgaaac cctctcggcg caggaggcgc aatcgctggc ggaacacgcc
    10741 ggcagcgaaa cgcaacggct gatttcgtcc gccgttgccg cgctcgaagg cggcgtgcat
    10801 cgcgtccaaa tcctcaacgg ggccgccgac ggcagcctgc tgcaagaact cttcacccgc
    10861 aacggcatcg gcacgtccat tgccaaagaa gccttcgtct ccatccggca ggcgcacagc
    10921 ggcgacatcc cgcacattgc cgccctcatc cgcccgctgg aagaacaggg catcctgctg
    10981 caccgcagcc gcgaatacct cgaaaaccac atttccgaat tttccatcct cgaacacgac
    11041 ggcaacctgt acggttgcgc cgccctgaaa acctttgccg aagccgattg cggcgaaatc
    11101 gcctgccttg ccgtctcgcc gcaggcacag gacggcggct acggcgaacg cctgcttgcc
    11161 cacattatcg ataaggcgcg cggcataggc ataagcaggc tgttcgccct ctccacaaat
    11221 accggcgaat ggtttgccga acgcggcttt cagacggcat cggaagccga gttgcccgaa
    11281 gcgcggcgca aagcctaccg caacaacgga cggaactcgc atattctggt gcgtcgcctg
    11341 caccgctgac cgcaacggaa agccgccgca gaaatgccgt ctgaaccccg tttcagacgg
    11401 catttccccg attataaaga cctgcccaac cctcaaggac cccgatgaaa tcctaccccg
    11461 acccctaccg ccattttgaa gacctcgatt ccgccgaaac gcaaaacttc gctgctgaag
    11521 cgaatgccga aacgcgcgcg cgttttttaa acaacgacaa ggcgcgcgca ttgtctgacg
    11581 gtattttggc gcagttgcag gacacgcggc agattccgtt ttgtcaggaa caccgtgcgc
    11641 ggatgtacca tttccatcag gacgcggaat atccgaaggg cgtgtaccgc gtgtgcacgg
    11701 cggcgacgta tcgttccggc tatcccgagt ggaaaatcct gttttcagtg gcggatttcg
    11761 acgagctgct cggtgacgat gtgtatttgg gcggcgtgtc gcacttggtg gaaaagccca
    11821 accgcgcgtt gttaacactg agcaaatcgg gcggcgatgc ggcgtacacg ctggaagtgg
    11881 atttggaagc aggggagttg gtagaaggcg gttttcactt tccggcaggt aaaaaccatg
    11941 tgtcgtggcg cgatgaaaac agcgtatggg tgtgtccggc ttgggacgaa cgccagttga
    12001 ccgaatcggg ctatccgcgc gaggtgtggc tggtggaacg cggcaaaagt ttcgaggaaa
    12061 gcctgcctgt gtatcaaatc gataaagatg caatgatggt gaacgcgtgg cgttatctcg
    12121 atccgcaggg ttcgccgatt gatttgattg aagcgtcgga cggtttttac accaaaacct
    12181 atttgcaggt gtcgtcggaa agcgaggcga aaccgttaaa cctgcccaac gattgcgacg
    12241 tggccggcta tctggccgga catcttttgc tgacgttacg taaagactgg aaccgcgcga
    12301 accaaagcta tccgagcggc gcattggtgg cggtgaagct gaatcggggc gaactcgggg
    12361 cggcacagct tttgtttgcg cccgatgaaa cgcaggcatt ggaaagcgtg gaaacgacca
    12421 agcgttttgt ggtggcgagc ctgctggaaa acgtacaagg ccgtctgaaa gcgtggcggt
    12481 ttgccgatgg caaatggcag gaagctgagt tgccgcgcct gccttcgggc gcgttggaaa
    12541 tgaccgatca gccttggggc ggcgacgtgg tttaccttgc cgccagcgat ttcaccacgc
    12601 cgctgacgct gtttgcgctg gatttgaacg tgatggaact gaccgtcatg cgccgccagc
    12661 cgcagcagtt tgattcagac ggcattaacg tgcagcagtt ttggacgact tcggctgacg
    12721 gcgagcgcat tccttatttc cacgtcggca aaaacgccgc gcccgacatg ccgacgctgg
    12781 tctatgccta cggcggtttc ggcattcccg aattgccgca ttatctgggc agcattggca
    12841 aatattggct ggaagagggc aatgcctttg tattggcgaa catccgcggc ggcggcgagt
    12901 tcggcccgcg ctggcatcag gcggcgcagg gcatcagcaa acataaaagc gttgatgatt
    12961 tattggcagt cgtgcgcgat ttgtccgaac gcggcatgag ttcgcccaga cacatcggtt
    13021 tgcagggcgg cagcaacggc ggcctgatta ccgccgctgc cttcgtgtgc gaaccgcaaa
    13081 gcatcggcgc gctggtgtgc gaagtaccgc tgaccgacat gatccgttat ccgctattgt
    13141 ccgccggttc aagttggacg gacgaatacg gcaatccgca aaaatacgaa gtctgcaaac
    13201 gctggctggg cgaattgtcg ccgtatcaca atctttcaga cggcatcgat tatccgcctg
    13261 cgctcattac caccagcctc agcgacgacc gcgtccatcc tgcccacgcg ctcaagtttt
    13321 acgccaaact gcatgaaacc tcggcgcaat cttggctcta ctcgcctgac ggcggcggcc
    13381 ataccggcaa cggcacccaa cgcgaatccg ccgacgaact cgcctgcgtc ttgctgtttt
    13441 tgaaagagtt tttgggctaa gggcggggag cggcactgcc gccgcgaatg aaaaaaggtc
    13501 gtctgaaact gctttttcag acgacctttt ttaatggttg tttcaaatca aaatatctat
    13561 gccgccggcc ccatcagcac ttcttcacat ccgaaggcaa aaatccgtaa tgccgtctga
    13621 acgcttcgtt gaaccgtccc gcgtggcggt agccgcaaaa gtgcatggcg gcttggacgg
    13681 tgctgccgga ttcgatgagg gcgagcgcgt gttccagccg caggcggcgc aggcatccgg
    13741 cgacggtttc accggtttgc gctttgaaat agcgtttcag gtagcattcg ttcagtccga
    13801 cgcggcgggc gatttcggcg atggtcagcg gacgggcgaa ttcgtgttgc aggatgtcgg
    13861 cggcttcgtc tatgcgccgg cggcggtaac cgttgtcgtg gcggcggaag gtgaagcgca
    13921 ataatcgggc ggagagttcc agcgcggcgg cttcgtcggc aagcaggccg aagccgtccg
    13981 attcgaacgg gcgttgcagc agtgggcagg ccgccgccgt cagtgccgct gcgttttgcg
    14041 ccagccgttg cagggcgaat cggcctattg tttgcggcga aaacaggcgt tcgtccagca
    14101 agccttcgtc gtgccagcgg cgcagttttt ccagcgaaaa atccaaatgc agcgcgcaca
    14161 tgccgctgtt gtcgggcagc agggtttcgg atacgtccgc caactcgccg cgtaccagcc
    14221 agatttcgcc ggcagacggg cggtattccc tgccgcccat ttgtaaccgg ttctgccccg
    14281 acaccatgac gaacaaggcg cagttgtggc tgaaattgtg gatttcggtg ggaaacgcgc
    14341 ccgttccgcc gccgcgcatc cgcgacaagg tgatgcccga gtcgaagcgg ttgatgcaca
    14401 tttccagatg caaaccgggc tgttttgcct gcgcaatgag cgcgctgtcg gaacagccgt
    14461 ccaacgccca gccggattta tcggagcgga cataggtttg gtactggcgg tagatggcgg
    14521 cggtgttcat gattggatag gaacgagttg tctaacaaat gaattaaata ggaattatta
    14581 ccaataatca agcgcaggga ttggttgaaa cggaaaaggt cgtctgaaag ggtgtttcag
    14641 acgacctttt ccgtatcggg aatttgtttt gccgtatcgg gaattttgcg ttttgcggcg
    14701 tggtttctgc aggttgtttg cttaataata aacattctta ttcgtatgca aaggaaccgc
    14761 acaccgtgaa accgcgtttt tattgggcag cctgcgccgt cctgctgacc gcctgttcgc
    14821 ccgaacctgc cgccgaaaaa actgtatccg ccgcatccgc atctgccgcc acactgaccg
    14881 tgccgaccgc gcggggcgat gccgttgtgc cgaagaatcc cgaacgcgtc gccgtgtacg
    14941 actgggcggc gttggatacg ctgaccgaat tgggcgtgaa tgtgggcgca accaccgcgc
    15001 cggtgcgcgt ggattatttg cagcctgcat ttgacaaggc ggcaacggtg gggacgctgt
    15061 tcgagcccga ttacgaagcc ctgcaccgct acaatcctca gcttgtcatt accggcgggc
    15121 cgggcgcgga agcgtatgaa cagttggcga aaaacgcgac caccatagat ctgacggtgg
    15181 acaacggcaa tatccgcacc agcggcgaaa agcagatgga gaccttggcg cggattttcg
    15241 gcaaggaagc gcgcgcggcg gaattgaagg cgcagattga cgcgctgttc gcccaaacgc
    15301 gcgaagccgc caaaggcaaa ggacgcgggc tggtgctgtc ggttacgggc aacaaggtgt
    15361 ccgccttcgg cacgcagtcg cggttggcaa gttggataca cggcgacatc ggcctaccgc
    15421 ctgtagacga atctttacgc aacgaggggc acgggcagcc tgtttccttc gaatacatca
    15481 aagagaaaaa ccccgattgg attttcatca tcgaccgtac cgccgccatc gggcaggaag
    15541 ggccggcggc tgtcgaagta ttggataacg cgctggtacg cggcacgaac gcttggaagc
    15601 gcaagcaaat catcgtcatg cctgccgcga actacattgt cgcgggcggc tcgcggcagt
    15661 tgattcaggc ggcggagcag ttgaaggcgg cgtttgaaaa ggcagaaccc gttgcggcgg
    15721 ggaaagagta gggagtcgtc tgaaaacgga gcttccgaag gaagcggggg gtttctgcga
    15781 agctaaagtg cggtttcaac gaattgaaaa gcagcctgta ttttgaaaat accgctcaag
    15841 caaacctacg gtttgccgcc ctctccctag ccctctccca cagggagagg ggattgggtt
    15901 gcaggctgcc tttaaggttt aggcaaattt ttaacttcgt tgaagctccg ttttcagacg
    15961 accttttgga acaccacagg cacacgcatc gaacggctga atcaaagatt cagaccgatg
    16021 gcagtccgca cccgagttta tgcggcaaac agcgaggcta cggcaacccg ccccctctcc
    16081 ctgtgggaga gggttaggga gagggcggta agccgcaggc ttacatcaaa gtcgataacg
    16141 ctttcgttac aactccgccc actgaacgaa gccaaaacga caaaccgcat cgtaaaccac
    16201 ccaacccata ggagaacccc atgcaaaacg aaaccatcaa cctgaaacag caccttgccg
    16261 ccatcaaaga atactggcag cccgaaatca tcaaccgcca cgggttccaa ttccgtttgg
    16321 tcaaactttt gggcgattac ggctggcata cgcacggata cagcgacaaa gtgctgtttg
    16381 ccgtggaggg cgacatggcg gtggacttcg ccgacggcgg cagcatgacg atacgcgagg
    16441 gcgagatggc ggtcgtgccg aagtcggtgt cgcaccgccc gcgttcggaa aacggctgct
    16501 cgttggtgct gattgagttg tccgacccgt ccgaggccgt ctgaaaacga agtttccgaa
    16561 ggaagctgag tttctgcgaa gctaaaagca gcctgcacct tcaatcaata tgccgaaaat
    16621 acaaccccac cgcacaccaa cacacaaagg aaatcccatg acacgcttca aatattccct
    16681 gctgtttgcc gccctgttgc ccgtgtacgc gcaggccgat gtttctgttt cagacgaccc
    16741 caaaccgcag gaaagcactg aattgccgac catcaccgtt accgccgacc gcaccgcgag
    16801 ttccaacgac ggctacactg tttccggcac gcacaccccg ctcgggctgc ccatgaccct
    16861 gcgcgaaatc ccgcagagcg tcagcgtcat cacatcgcaa caaatgcgcg accaaaacat
    16921 caaaacgctc gaccgcgccc tgttgcaggc gaccggcacc agccgccaga tttacggctc
    16981 cgaccgcgcg ggctacaact acctgttcgc gcgcggcagc cgcatcgcca actaccaaat
    17041 caacggcatc cccgttgccg acgcgctggc cgatacgggc aatgccaaca ccgccgccta
    17101 tgagcgcgta gaagtcgtgc gcggcgtggc ggggctgctg gacggcacgg gcgagccttc
    17161 cgccaccgtc aatctggtgc gcaaacgccc gacccgcaag ccattgtttg aagtccgcgc
    17221 cgaagcgggc aaccgcaaac atttcgggct ggacgcggac gtatcgggca gcctgaacac
    17281 cgaaggcacg ctgcgcggcc gcctggtttc caccttcgga cgcggcgact cgtggcggcg
    17341 gcgcgaacgc agccgcgatg ccgaactcta cggcattttg gaatacgaca tcgcaccgca
    17401 aacccgcgtc cacgcaggca tggactacca gcaggcgaaa gaaaccgccg acgcgccgct
    17461 cagctacgcc gtgtacgaca gccaaggtta tgccaccgcc ttcggcccga aagacaaccc
    17521 cgccacaaac ttgggcgaac agccgccacc gtgcgctcaa cctgttcgcc ggcatcgaac
    17581 accgcttcaa ccaagactgg aaactcaaag ccgaatacga ctacacccgc agccgcttcc
    17641 gccagcccta cggcgtagca ggcgtgcttt ccatcgacca caacaccgcc gccaccgacc
    17701 tgattcccgg ttattggcac gccgacccgc gcacccacag cgccagcgtg tcattgatcg
    17761 gcaaataccg cctgttcggc cgcgaacacg atttaatcgc gggtatcaac ggttacaaat
    17821 acgccagcaa caaatacggc gaacgcagca tcatccccaa cgccattccc aacgcctacg
    17881 aattttcccg cacgggtgcc tacccgcaac catcatcgtt tgcccaaacc atcccgcaat
    17941 acggcaccag gcggcaaatc ggcggctatc tcgccacccg tttccgcgcc gccgacaacc
    18001 tttcgttgat actcggcggc agatacagcc gttaccgcac cggcagctac gacagccgca
    18061 cacaaggcat gacctatgtg tccgccaacc gtttcacccc ctacacaggc atcgtgttcg
    18121 acctgaccgg caacctgtcg ctttacggct cgtacagcag cctgttcgtc ccgcaatcgc
    18181 aaaaagacga acacggcagc tacctgaaac ccgtaaccgg caacaatctg gaagccggca
    18241 tcaaaggcga atggcttgaa ggccgtctga acgcatccgc cgccgtgtac cgcgcccgta
    18301 aaaacaacct cgccaccgca gcaggacgcg acccgagcgg caacacctac taccgcgccg
    18361 ccaaccaagc caaaacccac ggctgggaaa tcgaagtcgg cggccgcatc acgcccgaat
    18421 ggcagataca ggcaggttac agccaaagca aaacccgcga ccaagacggc agccgcctga
    18481 accccgacag cgtacccgaa cgcagcttca aactcttcac tgcctaccac tttgcccccg
    18541 aagcccccag cggctggacc atcggcgcag gcgtgcgctg gcagggcgaa acccacaccg
    18601 accctgccac gctccgcatc cccaaccccg ccgccaaagc ccgcgccgcc gacaacagcc
    18661 gccaaaaagc ctacgccgtc gccgacatca tggcgcgtta ccgcttcaat ccgcgcgccg
    18721 aactgtcgct gaacgtggac aacctgttca acaaacacta ccgcacccag cctgaccgcc
    18781 acagttacgg cgcactgcgg acagtgaacg cggcgtttac ctatcggttt aaataaggtc
    18841 gtctgaaaac ggagtttctg cgaag
//
LOCUS       DAWGJV010000026        18835 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWGJV010000026 DAWGJV010000000
VERSION     DAWGJV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.026"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(161..532)
                     /locus_tag="WHQ94_000633"
     CDS             complement(161..532)
                     /locus_tag="WHQ94_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127961.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(666..1109)
                     /locus_tag="WHQ94_000634"
     CDS             complement(666..1109)
                     /locus_tag="WHQ94_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127962.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1152..2021)
                     /locus_tag="WHQ94_000635"
     CDS             complement(1152..2021)
                     /locus_tag="WHQ94_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ2127963.1"
                     /translation="MKRIFLPALPAILPLSAYTDLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2185..3109
                     /locus_tag="WHQ94_000636"
                     /pseudo
     CDS             2185..3109
                     /locus_tag="WHQ94_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
     gene            complement(3187..3828)
                     /locus_tag="WHQ94_000637"
     CDS             complement(3187..3828)
                     /locus_tag="WHQ94_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2127964.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3883..4674)
                     /locus_tag="WHQ94_000638"
     CDS             complement(3883..4674)
                     /locus_tag="WHQ94_000638"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ2127965.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTHQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4840..4915
                     /locus_tag="WHQ94_000639"
     tRNA            4840..4915
                     /locus_tag="WHQ94_000639"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4873..4875,aa:Thr,seq:tgt)
     gene            4950..5025
                     /locus_tag="WHQ94_000640"
     tRNA            4950..5025
                     /locus_tag="WHQ94_000640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4983..4985,aa:Thr,seq:tgt)
     gene            5167..5958
                     /gene="panB"
                     /locus_tag="WHQ94_000641"
     CDS             5167..5958
                     /gene="panB"
                     /locus_tag="WHQ94_000641"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ2127966.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGSKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHNSVQAAVRAYVEEVKAKTFPAAEHIFTD"
     gene            6081..6917
                     /locus_tag="WHQ94_000642"
     CDS             6081..6917
                     /locus_tag="WHQ94_000642"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ2127967.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLELVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7082..8920
                     /locus_tag="WHQ94_000643"
     CDS             7082..8920
                     /locus_tag="WHQ94_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127968.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLEKAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9060..9641
                     /locus_tag="WHQ94_000644"
     CDS             9060..9641
                     /locus_tag="WHQ94_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ2127969.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9651..10496
                     /gene="ispE"
                     /locus_tag="WHQ94_000645"
     CDS             9651..10496
                     /gene="ispE"
                     /locus_tag="WHQ94_000645"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ2127970.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            10504..10579
                     /locus_tag="WHQ94_000646"
     tRNA            10504..10579
                     /locus_tag="WHQ94_000646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10537..10539,aa:Gln,seq:ttg)
     gene            10592..10667
                     /locus_tag="WHQ94_000647"
     tRNA            10592..10667
                     /locus_tag="WHQ94_000647"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10625..10627,aa:Gln,seq:ttg)
     gene            10676..10751
                     /locus_tag="WHQ94_000648"
     tRNA            10676..10751
                     /locus_tag="WHQ94_000648"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10709..10711,aa:Gln,seq:ttg)
     gene            10918..11901
                     /locus_tag="WHQ94_000649"
     CDS             10918..11901
                     /locus_tag="WHQ94_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ2127971.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11968..12540
                     /locus_tag="WHQ94_000650"
     CDS             11968..12540
                     /locus_tag="WHQ94_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ2127972.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEDQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12665..13834)
                     /locus_tag="WHQ94_000651"
     CDS             complement(12665..13834)
                     /locus_tag="WHQ94_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ2127973.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILAITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQIYGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQVLGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWVRLTGQ"
     gene            13982..15508
                     /gene="ilvA"
                     /locus_tag="WHQ94_000652"
     CDS             13982..15508
                     /gene="ilvA"
                     /locus_tag="WHQ94_000652"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ2127974.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15565..16638)
                     /locus_tag="WHQ94_000653"
     CDS             complement(15565..16638)
                     /locus_tag="WHQ94_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ2127975.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(16635..17495)
                     /gene="cysW"
                     /locus_tag="WHQ94_000654"
     CDS             complement(16635..17495)
                     /gene="cysW"
                     /locus_tag="WHQ94_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ2127976.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNTV"
     gene            complement(17865..18701)
                     /gene="cysT"
                     /locus_tag="WHQ94_000655"
     CDS             complement(17865..18701)
                     /gene="cysT"
                     /locus_tag="WHQ94_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ2127977.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         4448 a         4369 c         5298 g         4720 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tttgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaattttaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agataccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa atagtaagga atatatatat atgcatattc cttactattt tttcacaaat
      661 tatgcttatc tatgcttatt tccataaaaa gcatgccctc gaattttttg acttgagttc
      721 tggaaatgag aataatttga atggatttcc ttctgtccat gtctaataac attttgtctt
      781 ccccaagctt ggtttgctac cgctcttgct tgttgagatg ttcgtgcata gacattagct
      841 cctcttgata aagcttgtgc tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg
      901 cccgcatgca aaattcctat tgccaccaac ggtgccgcag ccccttcagt ttctttcatc
      961 tccttttgag aaagctccgc caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg
     1021 ttttcaaatg tctgattgtc caagccgttt gccattgcgg gggcaatcat agacagcatc
     1081 attacggctg cggtgatttg ttttttcata ataactcctt tggattacaa ggttggaaaa
     1141 tcaaaacctg cttaaaacgt atgctgtacg ccaaatttca gttcggaact gctttgccct
     1201 gaaacgttga aacgtgcgga tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg
     1261 ccgaaatgag cgtaggtgga tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg
     1321 ggctgtgtgc ccagccattg gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat
     1381 gagatgttgg ggttgagcag caggtagttg cccgatttgt agcggatgcc gtctgaaagg
     1441 gttttactgc cgttgatgcg gtaggcggcg gtgagggaaa ggacaatcgg atctatggct
     1501 ttgtaggtgg tggcgccgat gagccaggat tttcccgacg aggctttgtt gcgcgatttt
     1561 tcgtaaaccg tgctttcaag aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa
     1621 gtgtggctga tgccgaggga tacgtcggac atccgtttgt tgcgggtttt gccgttgccg
     1681 tcgagtttgc gttcttcgtg ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg
     1741 gtcagtccgt agcgcaaacc gagcgtgccg acgagcatat cggtattgct gccgttttct
     1801 tggatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
     1861 gcaagttcgg cgcggttgtt ttcgctgttc aggtaggtaa gggaagtttc cagtttccat
     1921 tttcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggtata agcggataaa
     1981 ggcaggatgg cgggcaaggc gggcaaaaag atgcgcttca tatttctcct attgatatta
     2041 attcttattt actgataata atgaatatcc gataatcttg tctaaataaa tttttaaatc
     2101 ataaaaaaca atatgtttgt ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg
     2161 tgaaaaacgg ttacaatccg ccgcatgaaa aaacgcaata atcctcttcc gctgttgaac
     2221 ggtgtcaaac ccagttattt ggtgctgccg catgaaaagc agttttacgg gctgccgctg
     2281 ctgcattttc tgtgcatccg ctttcctttt gtgggcgcgg acgattggcg caggcggttg
     2341 aacagcggtt ttgtggtcgg ttcggatggt gcggcgttgg acgaacattc tttgttcgag
     2401 tcgggtaagg tggtgtttta ttaccgtgaa accagccgtg agagcgagcc gcgtattccg
     2461 tttgaagaaa agattttgca tattgatgag catttgattg tggtggacaa accgcatttt
     2521 ctgcccgtca tccccagcgg caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg
     2581 cggcctgaat tgcagcattt gaatgttgag gacattacgc cgctgcaccg cttggacaag
     2641 gatacggcag gcgtgatgct gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg
     2701 atgtttcaaa acaaaacggt atggaaaacg tatgaggcgc ttgcgccgac aaggacggat
     2761 ttgccgtatc cgattgatgt ggtttcgcgt ttggtaaggg gtgagaaatt ttttacgacg
     2821 caagaggcgg aaggcgaacc aaacgcacat acgacggtcg aactgattga aaacaggggg
     2881 ggaattcagc ctttaccgcc ttacgccgca taccggcaag aaacaccagt tgcgcgtgca
     2941 tatgatgggt ttgggtatgc cgctgttgaa tgacgcgctc tatcccgtgc cgtctgaagc
     3001 gggcagcgag gattatcgga aacctttgaa gttattggca aaaaagattg cgtttgcaga
     3061 tcctttgtcg ggtcgggaaa gggtgttttg cagtaggttt tgcctataat gagagggaat
     3121 gcaaacccgt atcggcagat gccgccgata cgggtttgat attatcgatg ccgtctgaaa
     3181 ggggttttat ccgttcaggc atacgggcgc gaagcggagg atggtgtcgc gcaggacgat
     3241 gagtttgccg tgaaagaaat gcgtggaacc ggcgatggta atgacgggca aatcttgcgg
     3301 ttccgcccat ttcagggctt tttcgatttc gacgacttcg tcttccgcgc cgtgtatcat
     3361 cagcgttttg gcaacattgg gaacggcgga cggctcgggg cggccggtat agtggcagac
     3421 tgccgcgccg atgagcagca ataaatcggg tatgcgcgct tgtgcggcaa atgtggcgac
     3481 ataaccgccg aaggagaagc cggataaggc aaattcgggg gcttcgggat gttgggcgcg
     3541 ggcatagtcg atgacggcga ggcagtcttg cgtttcgccg cgtccgtaat cgtgtgtgcc
     3601 ttcgctgttg cctacgccgc gaaggttggg caggtagcag tggaagccaa gtttgcttaa
     3661 ggctttggcg gcggtttgga tgactttgtt ggtgtttgtt ccgccctgga gggggttggg
     3721 atgattgatg acggcaacac cgcgtgccgg tacttgttcg gacgggatat ggatggtttc
     3781 taaaatgccg gcaggaccgg atatgtgtat ggtttcgggt ttcagcataa tgatttcctc
     3841 ttgattttcc gcatgttcca atgcagccgt gaagatagtt gcttaaaggt ggaaatgctt
     3901 gccgttgttg gggttggacg ctttcaggcc ggcaagcata cggtggaagt ccaatccgta
     3961 tgcgttgctc agtttgtcgg cacgtttttt gagtttgtct atgttttgct ctttggggct
     4021 gctttggaag gccattaccg cgacattgcc gtggctttcg gcaggaagtt ccaagacgcg
     4081 cccttcaaaa acgctcaaca accgttcgat gaagcgttgg tagcgtttgt cgccgctcca
     4141 ccagttggtt acgaatatgc cgtctgaaga gagtgcgttt cggcagtctc ggaaaaacgg
     4201 ttcttcaacc agtgcatcga tgatttgttc gccgtcgaac ccgtccacca aaatcacatc
     4261 ggtgttgtgg cggaagactt tgatatattc tgcaccgtct gcttcgataa tttcaaattt
     4321 ctcgccctcg aaaggcaact cgaacaggct gcgggcaatg gcgatgacct gcgggttgat
     4381 gtccacggcg gtttggtgcg tgtcgggcag gtaggtgtct atccagcgtg caaacgaacc
     4441 gccgcccaag ccgatttggg tgatatgctg cggaagagcg tcggtaaaca gcagccagcc
     4501 catcatggca cggctgtaag agagtaccag ctcggacggg tggtcgaggt tcattgagct
     4561 ttggatggtg tcgctgccca agtgaagcga acggatattg ccttcttcgg aaatgccgac
     4621 ttcgggaaag ctggattttg caggacgcag gcggcggtag ggatgtcttg ccatcggtgt
     4681 gaacggtcgg tttgaaaggc agatatttta tcagaatgcc gatggttgtc ctgcttcaaa
     4741 ttaatttctt ttcaaataaa ttacttattc ggatttgccg gggctttcgg ataaattcct
     4801 tgccaaggtg cggcattgcc tgcataattc gctttctttg ccggtatagc tcagttggta
     4861 gagcacctga cttgtaatca gggggtcccg agttcgactc ttggtgccgg caccatttct
     4921 gctgatggtg ttgcaaaaca tttcagtaag ccggtatagc tcagttggta gagcacctga
     4981 cttgtaatca gggggtcccg agttcgactc ttggtgccgg caccagattt gacagtccga
     5041 ttgtgtaaaa cagtcgggct gttttgcatt tgcggcacgg tcggtggcag ggctgcgggc
     5101 atttcactat aatagcccgt ttcaaactga aaaggccgtc tgaaaagggc ggggtaacaa
     5161 tatctgatga ttactgtgaa cacactgcaa aaaatgaagg cggcgggcga gaaaatcgct
     5221 atgctgaccg cttacgaatc cagttttgcc gcgctgatgg acgatgccgg cgtggatgtg
     5281 ctgctggttg gggattcttt gggaatggcg gttcaagggc ggcagtcgac gctgcccgtc
     5341 agcctgcgcg atatgtgtta tcacaccgaa tgtgtagcac gcggtgcaaa aaatgcgatg
     5401 attgtcagcg atttgccgtt tggtgcatat cagcagagta aggagcaggc gtttgccgcc
     5461 gccgccgaac tgatggctgc cggcgcgcat atggtcaaac tcgaaggcgg tgtgtggatg
     5521 gcggaaacta ccgaattctt gcaaatgcgc ggtattccgg tttgtgcgca tatcggtctg
     5581 acgccgcaat cagtgtttgc ctttggcgga tataaggttc agggtcgcgg aagtaaggcg
     5641 caggcgttgc ttaacgatgc caaggcgcat gacgatgcgg gggcggcggt cgtgttgatg
     5701 gagtgtgttc ctgcggaact ggcaaaaaag gtaactgaat ccgtttcttg tccgaccatc
     5761 ggtatcggtg cgggtgtaga ttgcgacggg caggttttgg taatgcacga tatgctcggt
     5821 atttttccgg gtaagacggc gaaattcgtc aaaaacttca tgcaggggca taatagcgtt
     5881 caagcggcgg ttcgtgcgta tgtcgaagaa gttaaagcaa aaacctttcc tgctgcagaa
     5941 catatcttta cagactgaac ggtttacatg cagcatgccg tctgaaagcc gtttcagacg
     6001 gcattttgtt ttgccgtgcg cggcgcgtat aatcggcgcg ttttgtcggg caggaagccc
     6061 gaaggataag gattaccgtt atgcaaatca tacataccat tcgagaactg cgcgcgtggc
     6121 gtaaaaatgc gggaaaggtg gcatttgtgc cgaccatggg caatctgcat gaagggcatc
     6181 ttgagcttgt gcgtgaggcg aaaaaacgcg cggacagtgt cgtggtcagc attttcgtca
     6241 accgcctgca attcggtcag ggcgaggatt tcgacaaata tccgcgtact ttgcaacagg
     6301 atgcggacaa gctggaagcc gagggtgttg ccgttgtttt cgcgcccgat gaaaaagaac
     6361 tctatccgaa cgtggagcaa cggtataacg tcgaaccgcc caacctgcaa aatgagttgt
     6421 gcggcaagtt tcgtccggga catttccgcg gggtggcgac ggtggtgtcc aagctgttca
     6481 atattgtcgc gccggatgtt gcctgtttcg gcaaaaaaga ctatcagcag cttgccgtga
     6541 ttaaaggttt gaccgaggat ttgaattttg atgttgaaat agtgcctgtc gatacggggc
     6601 gcgcggaaga cggtttggca ctctccagcc gcaaccagta tttgagtgcg gcggaacgcg
     6661 acgaagcacc gcgcctgtac cgcgaattaa aggctgttgc cgaatccttg gcgcagggca
     6721 gtttggatta tgcaggtttg gagcggcagg ctgccgacca tttgaccgct gcgggctggg
     6781 tggtcgatta tgtcgaaatc cgccgcgccg atacgctcga agtggcgcgg gcgggagata
     6841 agaaactggt ggtcttggcc gccgcccgtc tggggacgac gcgcctgatt gacaatttgg
     6901 aaataaaact cccttaaacc gcaagcgtcg ggaatgccgt ctgaagcgga tttgcgtttc
     6961 agacggcatt tatttttttg aacggggttt cgcaaatcta caaacgattc tgcttgtgat
     7021 aaagttacgc ctgattatat gccgtctgaa ggttcggacg gctgtcggat aaaggaagat
     7081 tatgttaccc gcccgtttca ccattttatc tgtgctcgcg gcagccctgc ttgccgggca
     7141 ggcgtatgcc gccggcgcgg cggatgcgaa gccgccgaag gaagtcggaa aggttttcag
     7201 aaagcagcag cgttacagcg aggaagaaat caaaaacgaa cgcgcacggc ttgcggcagt
     7261 gggcgagcgg gttaatcaga tatttacgtt gctgggaggg gaaaccgcct tgcaaaaggg
     7321 gcaggcggga acggctctgg caacctatat gctgatgttg gaacgcacaa aatcccccga
     7381 agtcgccgaa cgcgccttgg aaatggccgt gtcgctgaac gcgtttgaac aggcggaaat
     7441 gatttatcag aaatggcggc agattgagcc tataccgggt aaggcgcaaa aacgggcggg
     7501 gtggctgcgg aacgtgctga gggaaagagg aaatcagcat ctggacggac tggaagaagt
     7561 gctggctcag gcggacgaag gacagaaccg cagggtgttt ttattgttgg cacaagccgc
     7621 cgtgcaacag gacgggttgg cgcaaaaagc atcgaaagcg gttcgccgcg cggcgttgaa
     7681 atatgaacat ctgcccgaag cggcggttgc cgatgtggtg ttcagcgtac agggacgcga
     7741 aaaggaaaag gcaatcggag ctttgcagcg tttggcgaag ctcgatacgg aaatattgcc
     7801 ccccacttta atgacgttgc gtctgactgc acgcaaatat cccgaaatac tcgacggctt
     7861 tttcgagcag acagacaccc aaaacctttc ggccgtctgg caggaaatgg aaattatgaa
     7921 tctggtttcc ctgcacaggt tggatgatgc ctatgcgcgt ttgaacgtgc tgttggaacg
     7981 caatccgaat gcagacctgt atattcaggc agcgatattg gcggcaaacc gaaaagaagg
     8041 tgcttccgtt atcgacggct acgccgaaaa ggcatacggc agggggacgg aggaacagcg
     8101 gagcagggcg gcgctaacgg cggcgatgat gtatgccgac cgaagggatt acaccaaagt
     8161 caggcagtgg ttgaaaaaag tgtccgcgcc ggaatacctg ttcgacaaag gtgtgctggc
     8221 ggctgcggcg gctgtcgagt tggacggcgg cagggcggct ttgcggcaga tcggcagggt
     8281 gcggaaactt cccgaacagc aggggcggta ttttacggca gacaatttgt ccaaaataca
     8341 gatgctcgcc ctgtcgaagc tgcccgataa acgggaggct ttgagggggt tggacaagat
     8401 tatcgaaaaa ccgcctgccg gcagtaatac agagttacag gcagaggcat tggtacagcg
     8461 gtcagttgtt tacgatcggc ttggcaagcg gaaaaaaatg atttcagatc ttgaaaaggc
     8521 gttcaggctt gcacccgata acgctcagat tatgaataat ctgggctaca gcctgctttc
     8581 cgattccaaa cgtttggacg aaggcttcgc cctgcttcag acggcatacc aaatcaaccc
     8641 ggacgatacc gctgtcaacg acagcatagg ctgggcgtat tacctgaaag gcgacgcgga
     8701 aagcgcgctg ccgtatctgc ggtattcgtt tgaaaacgac cccgagcccg aagttgccgc
     8761 ccatttgggc gaagtgttgt gggcattggg cgaacgcgat caggcggttg acgtatggac
     8821 gcaggcggca caccttacgg gagacaagaa aatatggcgg gaaacgctca aacgtcacgg
     8881 catcgcattg ccccaacctt cccgaaaacc tcggaaataa tgcaggtcca tcctttcaga
     8941 cggcataagg tttgccggga agccggggca ttcgggcaaa cggcacgcag ttcgcacgcg
     9001 ttttgcacgg cacgccgaac ccatcggccg gcaggatggc atccgttaag gaaattctga
     9061 tgaaacacac cgtatccgca tcggtcatcc tgcttttgac cgcttgcgcg caattgcctc
     9121 aaaataacga aaacctgtgg cagccgtccg aacacacccg cagtttcacg gcagaagggc
     9181 ggttggcagt gaaagcggaa gggaaaggtt cgtatgcaaa tttcgattgg acataccaac
     9241 cgctcgtgga aaccatcaat atcaacaccc ctttgggcag tacgctcggg cagttgtgtc
     9301 aagacaggga cggcgcattg gcagtggacg gcaaaggaaa tgtctatcag gcggaaagtg
     9361 cggaagaatt gagcaggcag ctggtcggtt tcaaactgcc aatccaatat ctgcatatct
     9421 gggcagatgg caggcgtgtg gcgggcgcgc cttaccgcat cctgccggac ggcatattgg
     9481 aacaatacgg ttggactgtc ggcagaaccg ccgacagtgg ggggcaagtc cgaacgttgc
     9541 aactgaataa cggaaatttg aacatcaggc tggttttcac cgaaatcggt atgccgtctg
     9601 aaaccgaaac cccggaacgc tgtgcggcgc gcacgagata aggcggacag atgaatattg
     9661 cggacggacg gcaggcgttt cccgcacctg caaaactgaa tctcgatttg aggattactg
     9721 gaaagcggga agacggttat cacaatatcg aaagcatatt ctgcctgata gatttgcagg
     9781 ataccgtata tttgaaacta agggacgacg gcaaaatcat cctgcacaat ccggttgatg
     9841 gaatgccgca ggaagtagat ttaagctacc gtgccgcatc gttgctgcaa aaatatgcgc
     9901 gcaaccccgc cggcgtggaa atatggctgg acaaaaaaat cccgacaggg gcgggtttgg
     9961 gcggcggaag ctcggatgcg gcaaccgttt tgctggtgtt gaaccgttgg tggcagtgcg
    10021 gtctgacgca gcggcagctc attgattcgg gcgcggctct gggggcggac gtaccgtttt
    10081 ttattttcgg aaaaaatgcg tttgcgcggg gtataggcga caggctggac gaaatggata
    10141 ttccgaaaca gtggtatgtc atcgtcaaac cgcccgtcca cgtttccact gcaaaaattt
    10201 tcacacacga aagcttgaca cgggattctg cctcaagcat aatgccgact ttccaaaacc
    10261 tgcaaccgtt tagaaatgat atgcaggcag ttgtatttaa agaataccct gaagtttgga
    10321 aagcctattc cgagttgtcc cgatatggat ttgccttaat gacaggttct ggtgcgtgtg
    10381 tattcacggc gtgtcaagat aggaatagcg catacaatat ataccgacaa gtttcagatt
    10441 tgtacgaggc atatttggca gagggtcttt caaaacatcc ttggttgtcc gtataaacat
    10501 tgttggggag tcgtcaagcg gttaagacac tggattttga ttccagcatg cgaaggttcg
    10561 aatccttcct ccccagccaa gtcaaacgag ttggggagtc gtcaagcggt taagacactg
    10621 gattttgatt ccagcatgcg aaggttcgaa tccttcctcc ccagccagcc ctttttgggg
    10681 agtcgtcaag cggttaagac actggatttt gattccagca tgcgaaggtt cgaatccttc
    10741 ctccccagcc aaataaaagc gtgtaagcct gcttacacgc ttttatttca tagaaataaa
    10801 aatattgaaa tgcctttgtt tgtgtcggat gttgcaggta taatgtcggg cttggtacaa
    10861 gcagagggaa gcattgtgtt ttctgagcgg aagttaaaca taaaatcagg tgagaatatg
    10921 gctgcgtacg acagtttgat ggtatttaca ggcaatgcca atcccgaatt ggcacaacgt
    10981 gttgtcaggc atttggacat ttctttgggc aatgcttccg tatccaagtt ttcagacggc
    11041 gaagttgctg tcgaactgtt ggaaaacgta cgcgggcgcg atgttttcat ccttcagccg
    11101 acctgtgcgc cgaccaatga caacctgatg gaaatcctga cgatggcgga tgcactgaag
    11161 cgtgcttcgg caggtcgtat taccacagcc attccgtatt tcggctatgc gcgccaagac
    11221 cgccgtccgc gttccgtccg cgttccgatt tctgccaaac tggtggcaaa tatgctgtat
    11281 tcggcaggga tcgaccgtgt tttgactgtc gatttgcatg ccgaccagat tcaaggtttc
    11341 ttcgatattc cggtggacaa tatttatgcc accccgattc tgttgaacga catcaaacaa
    11401 cagcggattg aaaatctgac cgtcgtcagc ccggacatcg gcggtgtcgt ccgcgcccgc
    11461 gccgtggcaa aatccctgaa tgccgacttg gcaatcatcg acaaacgccg cccgaaagcc
    11521 aatgtggcgg aagtcatgaa catcatcggc gatattcaag gcagaacctg tctgattgtg
    11581 gacgatatga ttgacaccgc aaatacgctg tgcaaagccg ccgtcgctct gaaagagcgg
    11641 ggggcggaac gtgtcttggc atatgccagc cacgccgtat tctccggaga ggcagtcagc
    11701 cgtattgcct catccgaaat cgaccaggtg gtcgtaaccg ataccattcc tttgtctgaa
    11761 gcggctaaaa actgcgaccg tatccgtcag gtaacgattg ccggtctgtt ggccgaaacc
    11821 gtccgccgca ttagcaatga agaatccgtc tcatatcttt tcaatgaaga agtgatgaca
    11881 ggcagcatgt tgctgccata agcccgaagc cgtcttaagc tggtcgcggc cgatgacggc
    11941 gattttacct aacttggagt atttaacatg acttatgaaa ttcaagcttc tgttcgtgaa
    12001 gcacaaggca ctggtgcgag ccgccgcctg cgtcgcgaag gccaaatccc cggcattctg
    12061 tacggtgaag accaagagcc cgttgcaatc gctgtggatc acaaaaccgt attctacgca
    12121 ttggaaaaag aatctttcca tactgcgttg attaaattgt ctctgaacgg tgaaaccaaa
    12181 gacgttatcg tccgcgattt ccaaatgcac ccgtttcgcc gcgaagttca acacatcgac
    12241 ttccaagctg tgaaagccga tcaacttgta cgcatccgtg ttcccctgca catcgttaac
    12301 gctgaaaatt cccaagctgt taaattgcaa ggcggccgtg tatctctgtt gaacacgact
    12361 gttgaagtgc ttgcattgcc tgccaatatc cctgctttct tggatttgga ttgtgccgaa
    12421 gtggttgccg gcgacattct gcacttgtca gacatcaaac tgcctgaagg tgtagaaagc
    12481 gtttccttga aacgtaacga aaatctggct gttgccaccg ttaccggtaa aaaacgctaa
    12541 ttgatttcag cagcagggcg gcgcgtatgc aatacgtacc gccctgttgt tttatgccgt
    12601 ctgaaccgtg tttcagacgg catttcttta tttgttggaa aagcgggata tttgaaacgg
    12661 cggattactg ccctgtcaga cgcacccaaa gcctttgcca ccggcttctt ttttttacat
    12721 tttccagtgc gacgatttct ttttcggcaa tggtgtatcc gttttgtctg attttgattt
    12781 ttcctaggac ctgccctttt tttaccgggg cgggaatcgg ctgtatggtt tccaaaatct
    12841 gttctgccat tttcgcttcc ttatgtggca gagtgatgta ggcttctttg aggaagcctg
    12901 cgcggacggt ttttttgctg cctccgtaaa tttggatttg ggcaacggtt tttcctttcg
    12961 gatatatttt gggcgtatcg aaggcctgca atgcccagtt cagcagcttg ctgttgtccg
    13021 atgcgcgtgt ttccgccgat tccgaaccca atgtgatggc aaggatgtgc ctgccgttgc
    13081 cggagtatga cacggcaagg ttgtagccgc cgctttctgt gtgtccggct ttcagaccgt
    13141 ttacattgtt gtccctatat aaaaggatat tgcggttgtt ttgttctata tttttgaatt
    13201 tgaaagattt gatggaaaac agcgggtaat attccggaaa gtcgcgcatc aatgcttcag
    13261 acagcagggc gaggtctttg gcggtggaaa cctgtccttc tctactcaag cctgtcgggt
    13321 ttttgaatac agtgttcttc atgcccaagc gtcgggcttc tttgttcatt tgttgcacaa
    13381 aattttcaat cgagccgttg cccagccggc cggcaagggt tagggcggca tcgtttgcgg
    13441 atagtgcaat catgcctttt aagagtttgt cggtgctgac cgtatcgccg ggacgtacaa
    13501 acattctgct tccttctgaa gcccatgcgg attcgggtat ttttaagttt tcttcagatc
    13561 ggatatttcc cgatttcatg tttttgaaaa ccagatatgc ggtcatcagt tgggttagtg
    13621 ccgccggttc gacaggggta ttgatgtttt tggcggataa aatctgtttg ctttgaaggt
    13681 cgataacgat gtgtgccgct gtgagggttt cgggtgtttg gaacgtgggg gcggcgtgta
    13741 ccgccggttt gttgggcgcg ggcgatgcgg ccgttgcgtg agaaacgcct aagatgatgg
    13801 aaagcaggac gggcaggatt ttatgtgctg tcatgaaata ttctaattgt gtgcgtgttt
    13861 cagtctgccg attatacgct tagggtgtct gatcgggcgg atttttcttg ctttcgcgcc
    13921 gtcttgggcg tatggttttg ggttttgcga ttttaataaa ccgattatcc catattgaat
    13981 tatgaacacg ccccttcctt attccaatta cctcatccgc atcctgacgg catctgtcta
    14041 tgatgtcgcg gtcgaaacgc ctttggaacc ggcacgcagc ctttctgtac gtttgaaaaa
    14101 caacatcctt ttgaaacgcg aagatttgca gccggttttt tcgttcaaaa tacgcggcgc
    14161 gtacaacaaa atgtccaagt tgccgaaaga tgcgctcgct tgcggcgtga ttgcggcaag
    14221 cgcgggcaat catgctcaag gcgtggcatt gtccgcacag cgtttgggct gccgtgccgt
    14281 tatcgttatg ccggagacta cgccgaaaat caaagtggat gcggttaaga gccgtggcgg
    14341 cgaggtggtt ttgcggggcg tttcatacaa cgatgcctac gattatgcga tggagttggc
    14401 ggaaaaagaa gggttaacct atatcgcgcc gtttgatgat cctgatgtga ttgcgggaca
    14461 ggggacggtg gggatggaaa ttgtcagcca gcatcccgat ccaatccgcg ccgtattcgt
    14521 accgataggc ggtggcggtt tggcggcggg cgtggcggca tttatcaagc aggtccgtcc
    14581 cgaaatcaaa gttatcggcg ttcagaccaa cgattcctgc tgtatgaagc agtcggtcga
    14641 agcgggtgaa atcgtccatt tgaaagatgt cgggctgttt tcagacggca cggcggtcaa
    14701 agtcgtcgga aacgaaacct tccgtctctg caaagaactt ttggatgaaa tcattacagt
    14761 cgataccgat gcggtttgcg gcgcggtcaa ggatattttc gatgacacgc gcagcattac
    14821 cgagccggcg ggcgcgttgg cgttggcggg tctgaaagcc tatatcgccc gagaaggcgc
    14881 ggaaaaccaa accctgattg ccgttaccag cggtgcgaat atgaattttc accgtttgcg
    14941 ccacgtttcg gaacggagcg aattgggcga gggcaacgaa ggtatttttg ccgttaccat
    15001 ccccgaagag cgcggcagct tccttaagtt tgtcaatata ttgggaaata ggaatattac
    15061 cgagttcaac taccgctacg gggacgatga aaaagcgcat atctttgtcg gacttcaagc
    15121 ggcaggcccg caggatttgg cggttatcgg cagccggttg gatgaggcgg gattgcccaa
    15181 tgtcgacctg accgatgatg agattgccaa aatccatatc cgctatatgg tcggagggcg
    15241 gacggacaaa gtagaaaacg agcgtctggt cagttttgag tttccggagc gtccgggggc
    15301 gttggcgcgt tttttgaacc atatgcaggg cggttggaac attacgctct tccattaccg
    15361 caaccacggt gcggattacg ggcggatttt ggtcggtatc gatgtgccgc cgcacgatgc
    15421 cgccgcattt gacggtttct tggaaagtct gggatacagc tatcacgagg aaacgcaaaa
    15481 tgccgcgtac aagctgtttc ttgcctgacg cttgaaagca caatgccgtc tgaaagcctt
    15541 tcagacggca ttgcgctttc atggttaaat cgaatattca atcagttcgt tttgagagaa
    15601 gacataaacc tgtttcggaa tcagcttcaa ttctttgcct tcggcgattg ggtaacgcgc
    15661 ggcatcgctg ccagccagcg tgatatgcac gtcttgtttg ccgtgtttca ccaaaatatg
    15721 cgtcaatgcg ccgacggcgt ggactttttc gatttcggca caaatcatcg gtgtttcgtg
    15781 ttcggcggcg atctgccatt cgtgcgggcg gatatagccg gtggcggttt gttcctgcca
    15841 tttgtattgt gcgtccaatt tccacgcgaa gccgttataa tgccagaagc ctttttcgat
    15901 gcgtccttca aaagcgtcgg tttcgccgag gaactcggta acgaaggcgt tttcgggttt
    15961 gcggtaaatg gcttcggcac tgccggtttg ttcgattttg ccgtggttca tcacgacgat
    16021 ttcgtcggaa acttcgaggg cttcttcttg gtcgtgcgta accagaatgc tggttacacc
    16081 caggttgtga tggatgtcgc gcagccaggt gcgtaattct ttgcgtactt tggcatccaa
    16141 cgcgccgaag ggttcgtcca aaagcaagag tttgggttcg accgcaagcg cgcgggcgag
    16201 ggcgatgcgc tggcgttgcc cgccggagag ttggtgcgga taggattttg ccaaatgaga
    16261 gagctgcacg agcttgagta attcttcgac tttggcgcgg atttgtcctt tggacgggcg
    16321 ttcggacttg ggcaatacgg tcaaaccgaa agcgacgttg tcaaacacgt tcatgtggcg
    16381 gaagagggcg tagtgttgga acacgaagcc gactttgcgc tcgcgcacgt gtttggcggt
    16441 tacgtcttgc ccgtcaaaga ggatttggcc gccgtcggcg ttttccagtc cggcgataat
    16501 gcgtaaaagc gtggttttgc cgcagccgga cgggccgagt agggaaacga gtttgccggt
    16561 ggggacgttg aggttgatgt ttttcagcgc gtgaaaattg ccgaagtgtt tgtttaagtt
    16621 ttggatggtg atactcatac tgtgttcctt tcggcggcgg cgagtttttt gtcttgtaat
    16681 ttggtaatga tgttttgcac cgccagcgtc gccagtgcta aaagtgccaa tacgccggag
    16741 agggcgaatg cgccggtaaa gttgtattcg ttgtagaaga tttcgaccaa aagcgggacg
    16801 gtgttggttt cgccgcgtat gtgtcccgat accacgctga ccgcgccgaa ttcgcccatc
    16861 gcgcgggcgt tggtgaggat gatgccgtag agtaacgccc atttgatgtt gggcagggta
    16921 acgcgccaaa acatctgcca gccgcttgcg ccgagtatca gcgcggcctg ttcttcgctg
    16981 tcgccctgcg cctgcattaa aggaatgatt tcacgcgcga caaaggggaa ggtaacgaac
    17041 aaggtcgcca aaataatgcc cggaatggca aagataatct gtatgccttg cgcttcgagc
    17101 cggccaccca atgccgtgtg cgcgccgaac aataagacga acatcaaacc ggccaccacg
    17161 ggcgatacgg aaaacggcaa atcgagcagg gtagtcagca actgcttgcc gcgaaaatca
    17221 aaacgggtca gcagccacgc catcgccacg cccaagacgg cattgacggg gacgacaatc
    17281 agcgcggtaa tcagcgtcaa tttgatggca gaccacgctt cgggatcgtt taaggatttc
    17341 aggtacaaat cccaaccgcc ttttaaggct tcgtaaaaca cggcgacgag cggcacgacc
    17401 agcatcagca gcagaaagcc cagcgcggcg gcaatcagca acacgcgcag ccagcgcggt
    17461 tcggtcaggt tgggattggc ggaatagggt ttcatggcgg tggtgttccc cggttggttt
    17521 aaaatgtttg gaggtcgtct gaaaagggat tgaggggttt cagacgacct ttttgttcat
    17581 taagtagtag gtcggatact tgtatccgac aaggttgctg aatcgcaaat gtttgtcgga
    17641 tacaagtatc cgacctacga attctgtgtt ttgtggggcg gattcgtttt cagacgacct
    17701 tttattgatt aagggaataa aaaaacgtct agctgccgtc attcccgcgc aggcgggaat
    17761 ccaccacagg gcaacaggaa aacagaaaat aaataaggca gccgaaattc accaatggat
    17821 tcccgcctgc gcgggaatga cggtaacagg tatttcagac gacctcaacc cttcgcgccc
    17881 gaacgcctgc ccaacgccca ctgcatcacg ttcagcgcaa acagaatcac aaacgaaacc
    17941 agcagcataa acaacgccac cgccgacgcg ccctgtacgt cgaactgttc cagcttgccc
    18001 gtaataatca gcggcaggat ttcagaaacc atcggaatat tgcccgcgat aaaaatcacc
    18061 gagccgtatt cccccgttgc ccgcgcgaac atcattcccg cgccagtcaa gagtgccggc
    18121 gtgatttccg gcagcagcac gcggcggaac gtcgtaaagc ggtttgcgcc caaagtcgcc
    18181 gccgcttcct catattcgcc cgataattcc tccaacaccg gctgcacggc gcggacgata
    18241 aagggcaggc tgacgacgac cagcgcaatc caaatgccga cgggcgtaaa cgcgattttg
    18301 atgcccaaag gctcgaaaaa acggcctatc caaccgttgg gcgcatacag ggttgccaac
    18361 gcgatacccg taaccgccgt cggcagcgca aacggcaaat caaccagcgc gttcgccaga
    18421 cccttgcccg gaaattcata gcgcaccaat acccacgcca ccagcgtgcc gaacacgaca
    18481 ttggtcagca tcgcataaaa cgacatccgc aaactcagcc acaccgccgc caacacgttc
    18541 ggctcggcaa tcgtgttcca aaagccgccc cagccgattt ccgccgcctt cgccgccatc
    18601 atcgcaaacg gcaagaccac aagcagcgac aggcacaata cggtcagacc gaggctgagt
    18661 ttgaagccgg gcagtacgcc gggcgttttg agcgctaaca taaaacaatg ctgaaaataa
    18721 ggaaaaggaa ggactacttt aacgatgccg tccgaaaaac ggaaagaatg gaaagtttgg
    18781 tgcaaagacg aatttgttat aaagcggttg gcagtttctc aagcgggcgc gatgt
//
LOCUS       DAWGJV010000027        18623 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWGJV010000027 DAWGJV010000000
VERSION     DAWGJV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.027"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(470..745)
                     /locus_tag="WHQ94_000656"
     CDS             complement(470..745)
                     /locus_tag="WHQ94_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2127978.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(742..2025)
                     /locus_tag="WHQ94_000657"
     CDS             complement(742..2025)
                     /locus_tag="WHQ94_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2127979.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2181..3599)
                     /gene="glnA"
                     /locus_tag="WHQ94_000658"
     CDS             complement(2181..3599)
                     /gene="glnA"
                     /locus_tag="WHQ94_000658"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ2127980.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVFTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWSSGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3683..3832
                     /locus_tag="WHQ94_000659"
     CDS             3683..3832
                     /locus_tag="WHQ94_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127981.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            3910..4719
                     /gene="aroE"
                     /locus_tag="WHQ94_000660"
     CDS             3910..4719
                     /gene="aroE"
                     /locus_tag="WHQ94_000660"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ2127982.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERASAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVNPEIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFTPNIRPVIEYMKAM"
     gene            4721..5422
                     /gene="mtgA"
                     /locus_tag="WHQ94_000661"
     CDS             4721..5422
                     /gene="mtgA"
                     /locus_tag="WHQ94_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ2127983.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGIFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5625..5700)
                     /locus_tag="WHQ94_000662"
     tRNA            complement(5625..5700)
                     /locus_tag="WHQ94_000662"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5665..5667),aa:Phe,seq:gaa)
     gene            complement(5891..6625)
                     /gene="lptB"
                     /locus_tag="WHQ94_000663"
     CDS             complement(5891..6625)
                     /gene="lptB"
                     /locus_tag="WHQ94_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ2127984.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGENFKY"
     gene            complement(6699..7229)
                     /gene="lptA"
                     /locus_tag="WHQ94_000664"
     CDS             complement(6699..7229)
                     /gene="lptA"
                     /locus_tag="WHQ94_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ2127985.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7210..7791)
                     /gene="lptC"
                     /locus_tag="WHQ94_000665"
     CDS             complement(7210..7791)
                     /gene="lptC"
                     /locus_tag="WHQ94_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ2127986.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7788..8324)
                     /locus_tag="WHQ94_000666"
     CDS             complement(7788..8324)
                     /locus_tag="WHQ94_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2127987.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEETECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8389..9363)
                     /locus_tag="WHQ94_000667"
     CDS             complement(8389..9363)
                     /locus_tag="WHQ94_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ2127988.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLST
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9460..10515
                     /gene="tal"
                     /locus_tag="WHQ94_000668"
     CDS             9460..10515
                     /gene="tal"
                     /locus_tag="WHQ94_000668"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ2127989.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKTAYQDWEQYFTAPKFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            10669..10959
                     /locus_tag="WHQ94_000669"
     CDS             10669..10959
                     /locus_tag="WHQ94_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2127990.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            10976..11863
                     /gene="gluQRS"
                     /locus_tag="WHQ94_000670"
     CDS             10976..11863
                     /gene="gluQRS"
                     /locus_tag="WHQ94_000670"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ2127991.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRVIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQSVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(11933..12943)
                     /gene="dusA"
                     /locus_tag="WHQ94_000671"
     CDS             complement(11933..12943)
                     /gene="dusA"
                     /locus_tag="WHQ94_000671"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ2127992.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGITVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(12974..13465)
                     /locus_tag="WHQ94_000672"
     CDS             complement(12974..13465)
                     /locus_tag="WHQ94_000672"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ2127993.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHDDTEK
                     DARYIADKIVHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13539..14297)
                     /locus_tag="WHQ94_000673"
     CDS             complement(13539..14297)
                     /locus_tag="WHQ94_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2127994.1"
                     /translation="MKQKKTAATVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14368..15234)
                     /locus_tag="WHQ94_000674"
     CDS             complement(14368..15234)
                     /locus_tag="WHQ94_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2127995.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYR
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15283..15561)
                     /locus_tag="WHQ94_000675"
     CDS             complement(15283..15561)
                     /locus_tag="WHQ94_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ2127996.1"
                     /translation="MSAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15561..15851)
                     /locus_tag="WHQ94_000676"
     CDS             complement(15561..15851)
                     /locus_tag="WHQ94_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ2127997.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(15854..16384)
                     /locus_tag="WHQ94_000677"
     CDS             complement(15854..16384)
                     /locus_tag="WHQ94_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ2127998.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16590..>18623
                     /locus_tag="WHQ94_000678"
     CDS             16590..>18623
                     /locus_tag="WHQ94_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ2127999.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFDGLFGGSYTLLLAGGGAALIALLL
                     LLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLA
                     LDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADAD
                     WNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNH
                     IGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAET
                     APETPDFNAAADDLSALLQPAEAPSVEENITETVAETPDFNATADDLSALLQPS"
BASE COUNT         4396 a         5319 c         4717 g         4191 t
ORIGIN      
        1 cttacaatat aaaacaaaca aatatagtgg attaacaaaa accagtacgg cgttgtctcg
       61 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
      121 cactataagt tgacatattg tgcgatatcg agggctgtac cgtattttcc catcgtattt
      181 tcccatcgta ttttcccgtt ctttatatga gcggcaggca tcctcataaa agaagggata
      241 ccgataagga aaaatacccc agcataaaaa atatactgat taatcaatac aaatccaaca
      301 aacagttcat cgtattcttt tctgttggcg tataaaaaat actgatatat gccttcactt
      361 tcatagggcg acgctttatg taccgtcctg tgtgttgaaa cgtcggtttt ccttaccgcc
      421 tgccccgctt ttccctgatt gctgccaact gccgtccgaa cctgaaccgt caggtttcag
      481 acggcatttt ttccgcctgt tcccacaatt tccgtttttg tgcggcggtc agttttttga
      541 cggcgatttc ctgtttgagc gcagtgcctt tatccatccc gcctgcaacg atacgcatcg
      601 ccaccggttt gaatacgcgg gtatatttcg cgccttttcc ggccgtgtgg gcggcaagcc
      661 gctgttgcgg attcgggctg atgccgcaat agaacgcgct gttttcacac aatatcaggt
      721 aaacgctcca gtttgacgcg ttcatctgcc tgcatcctga gttttctccc cgttccaagg
      781 cgcaactttc aacagcagca gcatacccgg tagggcgagt gcgaaacaca gttggaaaaa
      841 cggtacataa ccgagccatt caatcagata accggcaaag gaattgatga ccgtgcgcgg
      901 gacggcggac aggctggtaa acagcgcaag ctgcgttgcg gtaaatgcgg gattggtctc
      961 gcgcgccata tacgatacga acgccgccgt ccccaaaccc acgccgactg cctctgcgcc
     1021 gataactgcc gccagcatca gcctctcgcc tgtgccgacc gtgtcgaaat gtccgaaccc
     1081 tgccagccat acaaacccca aaacggttac agcctgcacc gcgccgaata gccacaaggc
     1141 tttgtttacg ccgattttca gcatccacac gccgcccaag atacctgccg ccactgccgg
     1201 ccacagtcct gcatttttcg caatcaaacc gatgtctgtc ttgctgaaac ccatatccag
     1261 ataaaacggc gttgccaacg cggttgccat actgtcgccg agtttgtaaa ggaagataaa
     1321 cagcagcacg cacaccgccg aagcgatgcc cttgcgcgta aaaaattctt taaacggctc
     1381 taccacggtc tgcttcaacg ttttaggaac ggcaggcggc aacacgggtt cgcgcgcaag
     1441 aaacagcgtc atcagaaggc cgggcagcat aaataatgaa gtgataacaa atacttctga
     1501 ccacggcatc ctgtctgcca acaccaaact caatgaaccg ggaatcaggg cggcaatccg
     1561 gtaggcgttc acatggactg agttgcccaa acccaattcc tcgtccgaca aaatctcgcg
     1621 cctgaacgca tccaatacaa tgtcctgact ggcggaaaaa aaagcgacaa gcaccgacaa
     1681 gccggcaatc agcggcagat gattacgggg gtttaaaaag gcatatgccg ccaaagctgc
     1741 cagcaaccct gcctgcgtca gcagcatcca cccgcgccgc cgccccaaaa cgggcagcct
     1801 gaccgcgtcc atcagcggcg accacaaaaa tttccaagta aacggcagac cgattaacgc
     1861 catcagcccg atgctcttca aatccacctg ctcgctgcgc aaccacgccg gaatcaggtt
     1921 aatcagaaag tacagcggca gccccgaggt aaaccccgta aaaatacaga cgagcatatt
     1981 tcgggaaaag atttgcccga taaaacctga tttcgatgca gtcatcgttc gtgcagccaa
     2041 aaaaaacaaa agcctattgt agcaaatatc gcccgcctgc tgaaaaacac agcccctgag
     2101 ccgccgtttt gccgttccaa tgcaaaatgc cgtctgaaac tgtgttcaga cggcattgct
     2161 tttctgaaaa cccgacgtgc ttacaggctg taatacattt caaattccag cgggtgcggc
     2221 gccatgcgga tgcggcgtac gtcttcttct ttgaaagcga tgtagctgtc gatccagtct
     2281 ttgctgaaca cgccgccgcg caggaggaat tcgtggtcgg ctttgagggc ggccagtgct
     2341 tcttccaaag aagcgcaaac ggtcggcacc aatgcatctt cttccggcgg cagatcgtac
     2401 aggtttttat cggcagggtc gcccggatgg attttgtttt gaatgccgtc caaacccgcc
     2461 atcaacaggg cggcaaatgc caaatacggg ttggcggtcg gatcggggaa acgcgcttcg
     2521 atacggcgcg ccttgctgct gttcacggac ggaatgcgga tggaagcgga acggtttttg
     2581 gcggagtatg ccagtttggt cggcgcttca aagtgcggca cgaggcgttt gtaggagttg
     2641 gtggacggat tggtaatcgc gttcaaggct ttggcgtgtt tgatgatgcc gccgatgtag
     2701 tagagcgcgg tgtcgctcaa gccggcatag ccgtcgcctg cgaacaggtt ttgaccgtct
     2761 ttccaaatgg attggtgaac gtgcataccg ctgccgttgt cgcccataat gggtttgggc
     2821 ataaaggtgg cggttttgcc gaagttgtgg gcaacgtttt gaatcacata tttcatgtct
     2881 tgggtttggt cggcgcgttt gaccaaagta gcaaagcgcg tgccgatttc catttggctg
     2941 ccggtgccga cttcggagtg gtgcacttcc acttcgatgc cgagttcttc caaaatgttt
     3001 accatcgcgg agcgcaaatc ttggccgcaa tcaatcggag caacgggcgc gtaaccgcct
     3061 ttgacggtcg ggcggtggcc ggtgttttga ccgtccatgt gcaggccgct tgaccatgcg
     3121 ccgctttcgg aagtgatttc gtaacgggtt ttgtgcatgt cggtttcaaa ttctacgccg
     3181 tcaaaaacga agaattcagg ctcagggccg aaataggcgg tgtcgccgat accggaagat
     3241 ttcaaatagg cttcggcgcg gcgtgcgata gagcgggggt cgcggtcata gccttggccg
     3301 tcagccgggt cgataacgtc gcaggtaaag acaacggtgg tatcgtcgta gaaaggatcg
     3361 acgaaggctg tagacgcatc gggacgcaac tgcatatctg aagcctgaat acctttccaa
     3421 ccgccgatgg acgaaccgtc aaacgcctga ccgttttcaa accactcttc ggggtcttcc
     3481 aacacgatgc gcgcaggcac ggtaaagtgg tgctgcttgc ctttggtatc ggtaaagcgc
     3541 aaatcgacaa agcgggcttc gctttcttca atcaatttta cggcgttttt aatagacatt
     3601 tttcagctcc tggaaaaggg aaatagcaaa atatgcggaa cgcggcggct tgacgcaaag
     3661 tcggcatgat tctaccacgt ccatgccgtc tgaaactcgc cctgcgtcaa atttcaagct
     3721 ggaaaatata gtttcagacg gcaagattgt caacaatatg cttctgtttg tctgttcaaa
     3781 agcggaatac ccgccctttt gtccgaatgc cgacaaaaca gatgccgtct gaaagcagtt
     3841 cagacggcat cgttcccatc ttcgcgccgc cgttataaaa tgaccgcccc gccttgatgg
     3901 aatatgacta tgcacgccct cccccgctac gccgtttttg gcaaccccgt cgcccacagc
     3961 aagtcgccgc agattcatca acaatttgcc cttcaggaag gcgttgacat cgaatacgaa
     4021 cgcatttgcg ccgacatcgg cggtttcgcg caggcggttt cgacattttt tgaaacaggc
     4081 ggttgcgggg caaacgttac tgtaccgttc aagcaggaag cgtttcatct ggcggacgag
     4141 cattccgaac gcgcatcggc ggcaggcgcg gtcaacacgc tgattttgct gaaaaacgga
     4201 aaactgcgcg gcgacaatac cgacggtatc ggtttggcca acgacatcac gcaggtcaaa
     4261 aacattgcca tcgaaggcaa aaccatcttg cttttgggcg cgggcggcgc ggtgcgcggc
     4321 gtgattcctg ttttgaaaga acaccgtcct gcccgtatcg tcattgccaa ccgtacccat
     4381 gccaaagccg aggaattggc gcagcttttc ggcattgaag ccgtcccgat ggcggatgtg
     4441 aacggcggtt ttgatatcat catcaacggc acatccggcg gtttgagcgg tcagcttccg
     4501 gccgtcaatc ctgaaatttt ccgcgactgc cgccttgcct acgatatggt gtacggcgaa
     4561 gcggcaaaac cgtttttgga ttttgcccgg caatcgggcg cgaaacaaac cgccgacgga
     4621 ctgggtatgc tggtcggtca ggcggcggct tcctacgccc tctggcgcgg atttacgccc
     4681 aatatccgcc ctgttatcga atacatgaaa gccatgtaat atgttccgca tcgtcaaatg
     4741 gctgattgcc ctgcccgtcg gcatctttat ctttttcaat gcctatgtgt acggcaacat
     4801 catcacctac cgcgccgtcg cgccgcatcg gactgccttt atgtcgatgc ggatgaagca
     4861 gtttgaacag gaaggtcgcg atgtcgcact ggattaccgc tgggtaccct acaaccgcat
     4921 ctcggtcaac ctgaaaaaag ccctgattgc ttccgaagat gcccgtttcg ccggacacgg
     4981 cggcttcgat tggggcggca ttcaaaacgc catcaggcgc aaccggaaca gcggcaaagt
     5041 gaaggcgggc ggttcgacca tcagccagca gcttgccaaa aacctgtttt taaacgaaag
     5101 ccgcagctat atccgcaaag gcgaagaagc ggcgattacc gcgatgatgg aagctgttac
     5161 cgacaaagac aggatttttg aactgtattt aaactcaatc gagtggcact acggtatttt
     5221 cggcgcggaa gccgcgtccc ggtattttta tcaaataccc gccgccaaac tgagcaaaca
     5281 gcaggcggcg aaactgacgg cgcgcgtccc cgccccgctc tactacgccg accatccgaa
     5341 aagcaaacgg ctgcgcaaca aaaccaatat cgtgctcaga cgcatgggtt cggcagaatt
     5401 gcctgaaagc gatacggact gagtgccgcc gcaatataaa atgccgtctg aaatatcatt
     5461 cagacggcat atgctttctg cctttaccct cttctccaaa acgcaacggg gcggtcatta
     5521 tcatttttgc ccttctacat aacggtcaga cacaaaacgt gtctgacacg gacaacttcc
     5581 caaaaacggc aggcaaaaaa aataaccgct tcaaggcggt tttttggtgg ccagaggcgg
     5641 aatcgaaccg ccgacacacg gattttcaat ccgttgctct accaactgag ctatctggcc
     5701 tttgtctgtc tcgttaagag atgcgtatta aaccaaatta cgggacgaca tgcaagcata
     5761 tcgggcaata ttctttgaaa acaggtttaa gtcaatgttt taaatttgaa ttaatttatg
     5821 taaaaacaac ataaatccgt atttttgccg cctgcccgac gagattaggc cacccgtctg
     5881 aaaaatattt tcaatatttg aagttctcac ccagataaac agaacgaacc tgttcgtttc
     5941 cgaccaaatc atcaggtttt cccgatgcca acaccgtgcc gtctgaaata atgtaggccc
     6001 gatcgcagat gctgagggtt tcgcgtacgt tgtggtcggt aatcaatacg ccgataccgc
     6061 gcgacttgag aaatccgatg attttctgga tgtcgatgac ggcgatggga tcgacgccgg
     6121 caaaaggctc gtccaacaaa ataaaatgcg gtttcatggc gagtacgcgg gcgatttcga
     6181 cgcgtcggcg ttcgccgcct gacaaagatg gggcaggatt attgcgcaaa cgctcgatat
     6241 tcaggtcggc aagcagtttt tcgatttccc tgtcgatttg atttttatct ttggttctga
     6301 tttccaagat ggcgcggatg ttttgttcga cggtcatttt acggaaaata gaggcttcct
     6361 gcggtaggta gccgacaccc aggcgggcgc gttcgtgtat gggcaggtga cgcaattcct
     6421 gcccgtccag catcacgctg cccgcatcgg cggcaatgag tccgactatc atgtagaagc
     6481 tggtggtttt acccgcaccg ttgggcccga gcagtccgat gacttcgccg ctttcgattt
     6541 cgagggagaa gcttttaacg acttggcgtt ttttaaaact tttttgcagg ttttgaacaa
     6601 caaggcggct gacgtttgcg ctcataaata tctcttcaat atcttcggtc ggcatatgcc
     6661 gtccgaaccg ggtttatgtt tcagacggca ttttgggatt attcggattt ttgcgtactc
     6721 gaaggctgga taacgacgct gaccctgccg gatttggaag cggattttgc gccggatttt
     6781 gtgctgccgc tgatggtata gacttcggtt ttggtgttgt atgtaatcac cgcaccttcg
     6841 gcgacatcgc cgccgcgctg tactttggca ttaccggtta agactacggt gctgcctgca
     6901 gatgaataag caacgttgtt tgcctgtccg cgcaccgtgc ctttgccgcc gtccaatgtc
     6961 tggctgaagc ggactggcga accttccgcc ctcacggatt cgccgccttt gccgccgcgt
     7021 gtaacattga cgcgggcggc ggaaatattg agcgtaccct gtctgatgac gacgtttccg
     7081 ctgaatgtgg tgctttggtt ggcttgatcg agcgaacctt ggtcggcctc aatctgaata
     7141 ggctgcctgc tgtcgctttg aagggcaaaa gcgggggacg ccgaaaaaaa tgctattaaa
     7201 acaaatagct tacatatctt ttgtatcata aatcgtggct ttcactttag atgagaagtt
     7261 caacatgcct gttttgtggt cgtaagtcat gccgcccgcc tgaccgtgcg atgcaccata
     7321 ttggaaactg acaggcgtat cggtttgggc atattgagat tcggtatcga cgtgcagctt
     7381 ttcggcttca actttacccg cctgccgttt gccgtcggcg gttttggtca gcacaacgtt
     7441 gtttttaaaa agaacctgtt tgttttcggt atggtaaacg gcttcgtcgc tgccgacttc
     7501 gtacaacaac ctgccttctt ggaagaacac gagatgcggc gaatcaaaat ggatgtcgct
     7561 gctttccgga aactgtttcg cgcccttcgc gctcaaatgt tctttcaagt atccctgttc
     7621 gtcaaaccgc ctgccgtcca agccgtccat tgtgtattgc ggttcgtcgg gattgagcct
     7681 gacttcttca atctcgactt cgctgatacg acccaaccat gccgacagac cgcccaaggc
     7741 aaccgccaat atcaatggga acgcaattcc gtaccgccat cttattttca tttgatgtac
     7801 tcgttcaaag ccgcgcccaa agtcccttgc gcctgcataa tcaggtcgca cacttcgcgc
     7861 accgcgcccg caccgccggg gcgtttggca atataggcgg cgtgttgcag cgtaaaccaa
     7921 tgcgcgtcgg ggacggcaac cggcaatccg cagcgcacca tcaccggcaa atcgaccacg
     7981 tcgtcgccga caaaggcaca ctcggtttct tccacgccga cctgagcgcg cagctcttca
     8041 tacgcggcgc gtttgtccga aatgcctttg aagtagtaat ttatgcccaa ctgtttgacg
     8101 cggatgccga cggagggcgc gtcccgacct gtgataatcg cagtctgcac gccgcttgcc
     8161 tgaagcattt tcagaccgtg tccgtccagt gtgtgaaacg atttgatttc ttcgccgtta
     8221 tcgcggataa agatgcgccc gtcggtcaaa acgccgtcca catccaggat caacagtttg
     8281 attttggcgg cgcgcgcctg taattcggga gaaattgcct gcatgggatt tccttgtttt
     8341 cagacggcat atcttaccac aaatgccgtc tgaaacggct gcaacgcctt atacaatccg
     8401 tgccgccagc aggtcgtgca tattcagcgc gccgatcagc acgccatctg catcggtaac
     8461 cagaagcccg ttcacatggt ttgcctgcat gactttcagg gcttcggtgg cgagacgttc
     8521 ggcggagatg gttttaggat gcgtatgcat gacttcgtct atcgaaagac cggtaaaatt
     8581 gtcgcattct tgaaacaggc ggcgcaaatc gccgtcggtg aatacgcctt tcagacggcc
     8641 ttgcccgtcc gttaccgcca acatgcccag ccctttttca ctcatgctga cgatggcttc
     8701 tttcaagggc gtgctgagtc ggacggcagg caggccgccg cctttgtgca taatgtcggc
     8761 aacgcgcaaa agtaggcgtt tgccgaggct gccggcaggg tggctcaagg cgaagtcgtc
     8821 gggcgtgaac gcgcgcgcgc gcaacagaac gaccgccaac gcatcgccca aagccatgac
     8881 ggcggtggtg ctggtggtcg gggcaagccc cagcgggcag gcttctttgg aaaccgacgc
     8941 cgtgatgtgg atgtcggcat ggcgcgccat ggttgaatcg gggcgggcgg tgatgcagac
     9001 gagggtaatg tctttgcgtt tgagtgcggg aatgatggcg gcgatttcat cgctttcacc
     9061 ggaattggaa atcgcgacga ccacgtcgtt gtccacaatc atacccaaat cgccgtgtgc
     9121 cgcttccgca gggtggacga aaaacgcagg cgtgccggtc gaggccatgg ttgccgccat
     9181 tttgcgcccg atatgtcccg acttgcccat gcccgtgata acgaccctgc ctttgcagtg
     9241 caacaacgcg tccgccgcaa gggcgaagtt ttcgtccaaa tccgccgcaa tttcgcgcaa
     9301 gccttccgct tcggtgtgca acacttcgcg cgcccaatcg agatattttc cgttttctgc
     9361 cattgccatc cgcctttgct gtaacaaaac tacccaaaat ttccgaatcg gggtagcata
     9421 agcctttgaa acatacacat ccatccaaag gaatcagaaa tgactatttt atcggacgtt
     9481 aaagcattag gacaacaaat ctggctggac aacctttccc gctcgctggt gcaaagcggc
     9541 gaattggcgc aaatgctgaa acaaggcgtg tgcggcgtaa cttccaatcc cgccatcttt
     9601 caaaaagcct tcgccggcga cgcgctttac gccgacgagg ttgccgccct caagcagcaa
     9661 gacctgaccc ccaaacaacg ctacgaaacg atggcggttg ccgatgtgcg tgccgcctgc
     9721 ggcgtctgtc ttgccgaaca cgaatccacc ggcggcaaaa ccggcttcgt cagcctcgaa
     9781 gtttcgcccg aattgtccaa agacgcgcaa ggcacggttg aagaagcgcg ccgcctctac
     9841 gccgccatcg ggtgcaaaaa cgcgatgatt aaagtgcctg ccaccgacgc aggcatcgat
     9901 gcgctcgaaa ccctcgtttc ggacggcatc agcgtgaacc tcacgctgct gttctcacgc
     9961 gcccaaaccc tcaaagccta cgccgcctac gcgcgcggca ttgccaaacg cttggcagct
    10021 ggacaaagcg ttgcccatat ccacgtcgtc gccagcttct tcatctcccg cgtggacggc
    10081 gcgctggaca caacactgcc cgaccacctc aaaggcaaaa tcgccatcgc ccttgccaaa
    10141 accgcctatc aagattggga acaatatttc accgccccca aatttgccgc actggaaacc
    10201 aaaggcgcaa accgcgtgca gcttttgtgg gcatctaccg gcgtgaaaaa cccagcctat
    10261 cccgacacgc tctacgttga cagcctgatc ggcgcgcaca ccgtcaacac cgttcccgat
    10321 gccacactca aagcctttat cgaccacggc acagccaaag ccacgctgac cgaaggcgta
    10381 gaagaagcac aagcgcagct tgctgaaacc gccgcgctcg gcatcgatgt cgaaaccttg
    10441 gcggcgcgtt tgcaggaaga cggtttgaaa caatttgaag aagcgtttga aaaactgctc
    10501 gcaccgttgg tttaaacttt tcatccccga tgccgtctga aaggctttca gacggcatcg
    10561 cccgtttgac cggacacagt gcggcatatc aaggattcac cctaaaaata ttaatcaaca
    10621 agaagttaat gcacgacatt acccaccaac acaaaaggaa atagaatcat ggcaaaagca
    10681 ctcgaaatca tttcacccaa tgaaatttat tcagatctga tttttaagga tccggtacct
    10741 ccccatacaa tatatacaga attgatgaaa ctgattggta gaaatgcaag taatgagaga
    10801 atcgcagata gagccttcga ttttttctcg tccggcctta tggatggttc agctaccgaa
    10861 gagcaatata atgccctata tgatttaacc ttacttgaag aacccgggat ggaactgaat
    10921 aaagatgaga taatggcttt gattaatagt cttaaataaa ggggccgtac cggatatgta
    10981 cacaggacgt ttcgccccca gcccgaccgg gctgctccac atcggctcgc tgctgaccgc
    11041 cgccgcttcc tatgccgatg cacgctcaaa cggcggcaaa tggctggtcc gcatggaaga
    11101 cctcgatcca ccgcgcgaaa tgccgggggc ggcaagccat atcctgcaca cgcttgaggc
    11161 attcggattc gagtgggacg gagaagtcgc ctatcagagc cgccgttacg ccctgtatga
    11221 agaaacccta tgccgcctga aaaccgccgg actggtctat ccctgccatt gcagccgcaa
    11281 agactggcag gccggggcaa ggcggggcgc agacgggttc gtctataacg gacgctgccg
    11341 ccaccccggt caacgccctg caccgcaagg caaacagccg gcgtggcgca tccgcgtccc
    11401 cgaccgcgtt atcggttttt cagacggcat cgtcggcggt tacgcccaaa acctcgccgg
    11461 cgacatcggc gatttcgtcc tgcttcgtgc agacggttac tgggcatacc aactcgccgt
    11521 cgttgccgac gatgccgaac agagcgttac ccacatcgtc cgcgggcaag acctgctcgt
    11581 ttctacgccg cgccaaatct atttgcagca gtgtttgggc gttccaacac ctcaatatgc
    11641 ccacctgccg ctgctgacca acgcgcaagg gcagaaatgg tcgaaacaga cgctcgcccc
    11701 cgcattggat ttaaaccgcc gcgaacaact cctccgccaa gtgttccgtt acctcaagct
    11761 gcccgaagca ccggaaaccg accgccctgc cgaactgctc gactgggcgg tggcacactg
    11821 ggatatgggc aaagtgccga aacacgccat taccgccccc taaacaccgc ccgacaaacg
    11881 cacaaatgcc gtctgaaacc ttcagacggc atttcataca cacagccccg ccctattcgc
    11941 gcatatttgc ccgttcgact tctttccacg cttcgagaat caggctgccg tcgttgtctt
    12001 tcaagagcgt tgcgtcggaa agcatacgcc gccaagtccg cgcgcctttc aagccgtgca
    12061 tcagcccgag gctgtggcgg acgatatggc gcaggattgt gccgcgtccg gcttggattt
    12121 gggcttggct gtatgtgtag aggcgttgca ccaaatcggc gtatccgatc gggttcgcag
    12181 tgtcgccgta aaacaatctg tcccattcgc gcatcaccat cgggttgtgg tacgcctcgc
    12241 gcccgaccat cacgccgtca acgtgttgca ggtgtccggc gattgcttcg ttggtggtga
    12301 tgccgccgtt gatgatgatt tccagcccgg gaaactcctg cttgaggcgg taaacataat
    12361 cgtatttcaa gggcggaacg tcgcggtttt ctttgggtga aagaccgtcc agccaagcgt
    12421 tgcgggcgtg gacgataaag gttttgcagg cggttttgtc gcgcagcgtg ccgacgaaat
    12481 cggcaacggt ttggtattcg gtctgcctgt ccacaccgat gcgatgtttg acggtaacgg
    12541 taatgccgac cgcgtcctgc atggcgttga ggcagtcggc aaccagcccg acttcgttca
    12601 tcagacacgc gccgaacgaa cctttctgga cgcgcggact gggacagccg cagttgaggt
    12661 tgacctcgtt gtagccgtat gcttcggcgg ctttggcggc tttcgccaaa tcggacggat
    12721 cgctgccgcc cagttgcagg gcgacgggct gctcgccttc attgaacatc aaaaagcggt
    12781 ctttgtcgcc gtaaacaatc gcgccggcgt tgaccatttc gctgtacaac caagcatttc
    12841 gggtaatctg gcgcgccata taacggtagt gcctgtccgt ccagtcgagc atgggggcga
    12901 cggacaggtg gcgcggggga agggtgtgtg tattgtcgtt cattgtcgat atttcaccat
    12961 tgcggattca atttcaatcc ggaacaacct tcatttttgg ggaaatccgc gtcatgaaag
    13021 agtccagcag tatggttacc ggcccatcgt tgcagagcga cacctgcata tgcgtgcgga
    13081 aacgccctgt ttcgacatga atcccgtgtc cgcgcaacag ttccgccgtt cgcaggtaaa
    13141 gctgctgcgc ctgttctgca ggtgcggctt gggaaaacga aggccgccgc ccgcttgccg
    13201 catcggcata aagtgtaaac tgcgacacca gcagcaccgc gccgccgaca tctttcaaag
    13261 acaggttcaa cttgcccgct tcgtcttcaa acacgcgcaa atggacgatt ttgtcggcga
    13321 tatagcgcgc atctttttct gtgtcgtcgt gcgttacgcc gagcagcacg acgaatccgc
    13381 cgtcgatttt gccacaggtt tccgtgccgt tttcggacac gacatccacc tttgcaccta
    13441 ccgttttctg tatgaccgca cgcataagcc gtcctcctta cctgaacgtg caatgccgtc
    13501 tgaaaaggct tcagacggca tcggtattgg aaatgccatt acggtttgac accgttttct
    13561 tccaaaatgg catcgatttt caagcgggct ttttcctgtc tcaaaccttg ttccaactgg
    13621 tttctgacca actcgaaagg ctgcgcgtcg gggtttttcc cgacctcgct gagtttgaac
    13681 agataatagc gttcgcccaa tttgaccgga tcgcgggtaa cgtcgccccg attcatcgcg
    13741 gcaaactgcg aagccagcgg ctcgggaagc tgctgcgcca taatgaaacc gtcaaaagcc
    13801 tgctcgtcgt tcggataacg cttcatcagc ccttcaaaag acagcccttt gagcaggagc
    13861 tgctgcgcct gacgcgcctc ctcttcggtt gcgaagctga cctgctgcaa tttgatcatg
    13921 cggatttgcc gctcataaaa ctgacgcagt gcgctttcgg aaaccgtttc cgaacgttcc
    13981 agaaaacgga cgtactcctc ggcataaaaa gacgcttcgg cgattttaaa gcggttttgg
    14041 acatccttat ccttatccaa accttccttc aatgccctgt ttttcaaaac ttccaaagtt
    14101 tgcagccgac ggacggcatc gtttcggatt gcctgcccgt ccggtttttg ggactgctcc
    14161 gcatgccggt ctgcctgctg catgatctgc gccaccagcg tatccaccaa agccgggtcg
    14221 atttcgggtg ctttggctgc cgcaaaacct gccaacattg cagcaataac tgtggcagcg
    14281 gtttttttct gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc tgtctgaacg
    14341 tcttgccgcc atatcccgtt ttcagaatta ttttgcaggt ttgatgtttg ccttgcccaa
    14401 cagtgcaccg acggcacggt caatccgttc cgcctgaagg ttgccggcaa tctgtccttt
    14461 catttcatca aaagaaggca cttttacctc gcggctgtcg ttgacataat aaacgccgta
    14521 gaaatcgccg tttttcagcg gcgttgccgt aaattcgcct tttttcaagt ccttaattgc
    14581 ccgataaagc ggcggaacac cctgttccaa atctttcagc ggcacatatc cgaccggcgc
    14641 gccggtctgt ttggtacggt cgttgaggga atattgtttc aagacggcat cgaaaccttt
    14701 tttcgccttc aagtcggcaa ccgctttttt tgcattttct tccttgtcgg tcaggatttc
    14761 gcccaactgg acttcctgcg tacctttgta aaaaccgctg atattgtcat atgcggcttt
    14821 tacttcctgc tcggaaaccg gttgggtttt ggcgatatgc agtgcgtatg cctcgccgtt
    14881 caagccatat tttaccgcct gccaaacggt tttgaaggac ggtttcttgt cgtcgcccga
    14941 ctttttcgct tcggcacgca atttggcaag cgcattttta aactctgccg accggtcgag
    15001 tttcaggcgt ttcacttcct gtgcgaccac ggtgttgacc acttcgtttt ccagcaggga
    15061 ttggcgcagt tgcggcgtgt cttcggcacg gctgttttcc gcacggaatg cggcaacctg
    15121 cgcatcgatg acggaactgt cgattttctg accgttgacg gttgccagcg tttgggcaaa
    15181 caggctgccg gaacaggcaa gcagtgcaac ggaagtcagg atttttgctt tcataatctt
    15241 ctctctttaa attttaaatt gaaatgttcg ggcagacttc attcagtccg ccgtatggaa
    15301 atactcgtca ggggtagccg cccggatgcc gagcgcgtga atcatgccgc ctgaaaacaa
    15361 atctttgagc agcgatttga ccgttttctg gcggttcagg cggcttacgc cttcaaaacg
    15421 gccgctgacg accaaaacgg cataatgtcc gccgccggta ttgcccgcgt gtcctttgtg
    15481 cagatggctc tcatcgccga tttccaacac cagcggatcg agcgtctgca ggcgttcgcg
    15541 gatcaaatcg acggccgaca ttatttgaac accgctttaa acggcttgat cagcacttcg
    15601 ctgtacacgc ctgcatgaac atagggatcg tcttccgccc aagcctgcgc cgcgtccaaa
    15661 gactcgaact gcgccacaat caaactgccc gaaacgcggt tggagtcctc cggcaaagga
    15721 ttcgggcctg ccgtcaacag ccggccttcc gacttcagcg tctccagccg tttgaggtgt
    15781 tcgggacgtg ccgccatacg cgcttcatac acattctccc cgtctgttgc cagcaacata
    15841 aaatattcca cagtcaatcc tcctttttca gacaggtact cagataaata ccctgaataa
    15901 taaagaaaac aagcatcagt gcagtcgagc cgaacatttt atagttgacc cattgcgact
    15961 cgaaccgggt aaacacaaac cagttggcga tacccataaa aatcaggaaa ccgacccaca
    16021 tatacgtcaa tttcgcccat acggcatccg gaagctgaat ctccctgccg atactcgcct
    16081 tcaagccgtt tttaccggcg aggtggctgc cccacaggaa cagcgcaccc agccaaaaca
    16141 aaacgctcgg cttccacata atgaagcggc tgtcgcccaa aacaatggtt gcgccgccga
    16201 atacgacaat cagcaccagt ccgacccact gcatcgtatc cagcttttta tatttccaat
    16261 acagaaaagc cgcctgaacc acaccggcaa ccaaggcaac cgccgtagcg gcaatcatat
    16321 ttttggtaac ggtataggtg gcgaaaaaca ggatgacgga caaaaggtcg ctgacgaatt
    16381 tcatatccaa atgagaaagc aaatcaaata aacgctaatc atacaggcag aaccctgttt
    16441 ttgcacctgc aaaaccaaca aattttgttt tgattgatgc aaatatgcac agatgttttt
    16501 gaaaaaagat ggagatatgt cataatttgt aaaaacggcg aatgattgcc gtttaaaatg
    16561 tggcggcggt cggtacattc acatattaaa tgcccgccgg ccgactgccc cgccgatgcc
    16621 cgatgatgac gaaatttaca gactgtacgc ggtcaaaccg tattcagccg ccaacccaca
    16681 ggggatacat cttgaaaaac aacagacaaa tcaaactgat tgccgcctcc gtcgcagttg
    16741 ccgcatcctt tcaggcacat gctggactgg gcggactgaa tatccagtcc aaccttgacg
    16801 aacccttttc cggcagcatt accgtaaccg gcgaagaagc caaagccctg ctaggcggcg
    16861 gcagcgttac cgtttccgaa aaaggtctga ccgccaaagt ccacaagttg ggcgacaaag
    16921 ccgtcattgc cgtttcttcc gaacaggcag tccgcgatcc cgtcctggta ttccgcatcg
    16981 gcgcaggcgc acaggtacgc gaatacaccg ccatcctcga tcctgtcggc tactcgccca
    17041 aaaccaaatc tgcactttca gacggcaaga cacaccgcaa aaccgctccg acagcagagt
    17101 cccaagaaaa tcaaaacgcc aaagccctcc gcaaaaccga taaaaaagac agcgcgaacg
    17161 cagccgtcaa accggcgtac aacggcaaaa cccataccgt ccgcaaaggc gaaacggtca
    17221 aacagattgc cgccgccatc cgcccgaaac acctgacgct cgaacaggtt gccgatgcgc
    17281 tgctgaaggc aaacccaaat gtttccgcac acggcagact gcgtgcgggc agcgtgcttc
    17341 acattccgaa tctgaacagg atcaaagcgg aacaacccaa accgcaaacg gcgaaaccca
    17401 aagccgaaac cgcatccatg ccgtccgaac cgtccaaaca ggcaacggta gagaaaccgg
    17461 ttgaaaaacc tgaagcaaaa gttgccgcgc ccgaagcaaa agcggaaaaa ccggccgttc
    17521 gacccgaacc tgtacccgct gcaaatactg ccgcatcgga aaccgctgcc gaatccgccc
    17581 cccaagaagc cgccgcttct gccatcgaca cgccgaccga cgaaaccggt aacgccgttt
    17641 ccgaacctgt cgaacaggtt tctgccgaag aagaaaccga aagcggactg tttgacggtc
    17701 tgttcggcgg ttcgtacacc ttgctgcttg ccggcggagg cgcggcatta atcgccctgc
    17761 tgctgctttt gcgccttgcc caatccaaac gcgcgcgccg taccgaagaa tccgtccctg
    17821 aggaagagcc tgaccttgac gacgcggcag acgacggcat agaaatcacc tttgccgaag
    17881 tcgaaactcc ggcaacgccc gaacccgctc cgaaaaacga tgtaaacgac acacttgcct
    17941 tagatgggga atctgaagaa gagttatcgg caaaacaaac gttcgatgtc gaaaccgata
    18001 cgccttccaa ccgcatcgac ttggatttcg acagcctggc agccgcgcaa aacggcattt
    18061 tatccggcgc acttacgcag gatgaagaaa cccaaaaacg cgcggatgcc gattggaacg
    18121 ccatcgaatc cacagacagc gtgtacgagc ccgagacctt caacccgtac aaccctgtcg
    18181 aaatcgtcat cgacacgccc gaaccggaat ctgtcgccca aactgccgaa aacaaaccgg
    18241 aaaccgtcga taccgatttc tccgacaacc tgccctcaaa caaccatatc ggcacagaag
    18301 aaacagcttc cgcaaaacct gcctcaccct ccggactggc aggcttcctg aaggcttcct
    18361 cgcccgaaac catcttggaa aaaacagttg ccgaagtcca aacaccggaa gagttgcacg
    18421 atttcctgaa agtgtacgaa accgatgccg tcgcggaaac tgcgcctgaa acgcccgatt
    18481 tcaacgccgc cgcagacgat ttgtccgcat tgcttcaacc tgccgaagca ccgtccgttg
    18541 aggaaaatat aacggaaacc gttgccgaaa cacccgactt caacgccacc gcagacgatt
    18601 tgtccgcatt acttcaacct tct
//
LOCUS       DAWGJV010000028        18336 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWGJV010000028 DAWGJV010000000
VERSION     DAWGJV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.028"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(56..1582)
                     /locus_tag="WHQ94_000679"
     CDS             complement(56..1582)
                     /locus_tag="WHQ94_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ2128000.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVSEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(1694..3100)
                     /locus_tag="WHQ94_000680"
     CDS             complement(1694..3100)
                     /locus_tag="WHQ94_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2128001.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLSISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            3384..4163
                     /locus_tag="WHQ94_000681"
     CDS             3384..4163
                     /locus_tag="WHQ94_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ2128002.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4160..4861
                     /locus_tag="WHQ94_000682"
     CDS             4160..4861
                     /locus_tag="WHQ94_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ2128003.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMCAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNISAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4858..6312
                     /locus_tag="WHQ94_000683"
     CDS             4858..6312
                     /locus_tag="WHQ94_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ2128004.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6495..6980)
                     /gene="purE"
                     /locus_tag="WHQ94_000684"
     CDS             complement(6495..6980)
                     /gene="purE"
                     /locus_tag="WHQ94_000684"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ2128005.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7028..7744)
                     /locus_tag="WHQ94_000685"
     CDS             complement(7028..7744)
                     /locus_tag="WHQ94_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ2128006.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            complement(7744..8412)
                     /locus_tag="WHQ94_000686"
     CDS             complement(7744..8412)
                     /locus_tag="WHQ94_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ2128007.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTVLALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8423..8608)
                     /locus_tag="WHQ94_000687"
     CDS             complement(8423..8608)
                     /locus_tag="WHQ94_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ2128008.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8751..10727
                     /gene="mutL"
                     /locus_tag="WHQ94_000688"
     CDS             8751..10727
                     /gene="mutL"
                     /locus_tag="WHQ94_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ2128009.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIGLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCTTMLERLALAHPHIAFSLKRDGKQVFKLPTQSLPERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELNRLEQARFGNMPSETPIPKT
                     DTPLSDGLPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     RENGKLQSQRLLIPSTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRTVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENRILATMSCHGSVRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10822..12951
                     /gene="dnaX"
                     /locus_tag="WHQ94_000689"
     CDS             10822..12951
                     /gene="dnaX"
                     /locus_tag="WHQ94_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ2128010.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    12011..12050
                     /gene="dnaX"
                     /locus_tag="WHQ94_000689"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            13031..13366
                     /locus_tag="WHQ94_000690"
     CDS             13031..13366
                     /locus_tag="WHQ94_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ2128011.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(14198..15244)
                     /gene="recA"
                     /locus_tag="WHQ94_000691"
     CDS             complement(14198..15244)
                     /gene="recA"
                     /locus_tag="WHQ94_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ2128012.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            15498..16262
                     /locus_tag="WHQ94_000692"
     CDS             15498..16262
                     /locus_tag="WHQ94_000692"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ2128013.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPIVTMSMGQTGTVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            16282..18297
                     /gene="rep"
                     /locus_tag="WHQ94_000693"
     CDS             16282..18297
                     /gene="rep"
                     /locus_tag="WHQ94_000693"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ2128014.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAEMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVVKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIIKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGVRIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQTEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         4508 a         5097 c         4957 g         3774 t
ORIGIN      
        1 tctttgagct aaggcgaggc aacgtcgtac tggtttttgt taatccgcta tatttctata
       61 ataaaccttc tatgggcagc agggatagga tttttgcggc gatgcgtttc caaagtttgg
      121 cttcgggttc gttcgggtag gtttttcggg tggcgggatc gtgccattgc aggcggttgt
      181 gcctgtcgag ggtaacgcgg taggcgtatt cgggtgtggt atcggcaagg gtgcgctcca
      241 tctgttctgc gattttgggg ctttcgatga cgacacccat ttcggtattg agccgtgcgg
      301 aacgggggtc gaggttgaat gagccgatga agatgcgttt gccgtccaca atgaaggttt
      361 tggcgtgcag gctggttacg gagctgccgg tcaggccttt gtcttttgtg gcggggacgg
      421 catggttggg ttgcagctcg tagagtttga tgccggcttt gagcaacggt tttcggtatt
      481 tgacatagcc agaatggacg gcggcaacgt cggtcgcctg tagcgagttg gtcagaacgg
      541 taacgtctat gccgtcctgc accagttttg ccagtgcgtc tgtgccggat tttgtgggga
      601 cgaaataggg tgaaaccaga tagacgcttt tttcgggctg tttgagcgcg tcttgcagcc
      661 gcccggcaat cggcggtttg cggcggtcgc ggtcgagtcc ttttgcaggg tcgtcgctga
      721 tgaggcgggt ttggacgctc tgccagtcga tgcgtcctgc ctgtattttt tggtagaggg
      781 gcgactgttc gacggtttcg cggtagcgca ggagcgcgtg tctggacgtt tcgtcgttgt
      841 atccgagtgc ttgaagaccc ttgccgatgt tgccgctgcg gatgatgcgc gtggcgttgt
      901 gggcggaatg gcttgcccag tagcggtcaa agtcgtgcga tacttcgcta acgacgctgc
      961 cggtggcgag gatgtccagg tcggcgaaaa cggtgtcctc accgactttg aagtattcgt
     1021 cgccgatatt gcgtccgccg agtatggtgg cgcggttgtc ggcggtaaag gatttgttgt
     1081 gcatgcggcg gttgaggcgg gggaagtcgg tcaggtagcc gagtgcgcgc cattttcgta
     1141 agacgaaggg gttgaacagg cgcacttcga tattgggatg gctgtcgagg gcgagcagga
     1201 ggtcgtccaa tccgcgcgtg ttgttgtcgt ccaacagcag gcgtacgcgc acaccgcgtt
     1261 ctgcggcaag gtacacgagg ttgaacagca gtcggccgga aatgtcgttg cgccagatgt
     1321 agtattgcaa atcgaggctg tgttcggcag attcgataag ggcggcgcgg gcggcaaagg
     1381 cttcgtgggg gtcgttcaac agatagatat cggatagccc gttggtatga ggggtgtgcc
     1441 ggatttgcag gatgttgtcc aggcggacgg gtttggaagt attgaaatga cggctttccg
     1501 tccgttcttc cagtgggggc aaccatgaag aacatgaaca gagaaggagg cataaaaggg
     1561 aaattaggct gcgtgttttc atcagggata tggtttcaga cggcattgcc tgtgttttgg
     1621 ggttggcgcg catggaagtg cggaatcata atccaaacgt tgaaacgggt aaaagttttg
     1681 cgtgcggacc gctttaggac ggtgtgttcc gtgtcaggtt tgcaccgtct gaaacgtgca
     1741 gacgcttgaa aaccaacgac gaggcaaggg taatgccgcc gatactgagc agggtcatac
     1801 ggaaggcgga atgcagacct gaagaagccg gtatcagaaa tgtccagttt ttaaggatta
     1861 atgcgccggc aacaatgccc atgctgatgg aaagctgttg gttgaccgcc atcaggctgt
     1921 tgccgctgcc ggtctgttgc gggcgcaaat cggcgagggt cagtgtgttc atggcagaaa
     1981 actgtaggga gttgcacgcg ccgatcgcca gcgagaggaa aatccaaatc cacagcggcg
     2041 agtttccatc aggcagggcg agcagcatga tgaaggcggc aagcagcttg gtgttccaaa
     2101 gcagtaccgt gcggtagccg aaacgtttca tgagcggtgc aatcagcggt ttgaccagca
     2161 gcgaagacag ggcgacgggg gcgaccagcc aacccgacag gcttgcgccg aagccgaaag
     2221 cgacttgaaa catcaggggc atcagaaaag gaatcgagct gatgccgaga cggctgaaca
     2281 gattgcccgc cagtcctaga cggaaagtgc gtatcagaaa caggtcggcg gaataaatcg
     2341 gtttggacgc ggttttcata tgtcggaaat aacggcgtgc aaacagcagt ccgccgcaca
     2401 gcggcaacag tgcaaaatac ggaggcagcg cgtgcgacag gctttctgcc gaaagtaaca
     2461 agaggcacgc ggcggcagaa aaaatcagat aacctttgaa gtctaaagag atattgctgc
     2521 ctttaatatc gggcatgatg ttgcgtccca atatgaaacc caaaagaccg atgggcaggt
     2581 tgagcaggaa aatccagtgc cacgaagcgt attcgaccag ataaccgccc gccaaaggcc
     2641 ctaaaaccgg tccgattaat gcgggcataa ccgcataatt gatggcgttg agcagcttgg
     2701 acttgtcgta cacacgcaag atggtcagac gcggtatcgg aaccagcatc gaaccgccga
     2761 tgccctgaac gacacgggaa agcgtcaatt caaacagcga acccgatgcg gcgcacaatg
     2821 ccgatccgag cataaaaacg gcaatcgaac cgaaaaagac ttttttcgtt ccgaacctgt
     2881 ccgccaaata accgctcaaa ggaatcagca gggcaaccgt cagcgtgtag gaaataaccg
     2941 ccagctgcat gttcaatggc gactcattca ggtcggcggc aatttcaggc agtgcggtat
     3001 ttaaaatggt cgcatccaac atctgcataa aaatggcaat tgccagcaga agcggcagcc
     3061 aaggggatgg tgcgcgggcg gatagggtgt ttttttccat agagcgattg taccccatcc
     3121 ttgtgccgtt attgttttca gatgctgtct gaatgccgtc agagtcggca tcttgaatgt
     3181 tcacaagcaa acgaaccggc attgcattgt aatgataatt attatcgaaa accatcagat
     3241 taaggtacag taagcgttat ggggtagttt gtaagaaaaa ccggattatt ttttaaaatt
     3301 agacttgacc cgcaacagtc aattacttaa agtaaacgct tacctttcta cagaaaaaaa
     3361 cgggtttccc gttatcaaaa aacatgagcg caaccattcc cccaaaaatc atccgatacg
     3421 acagcaatcc gaccgatgtc tattttttcg gcacttgcgt ccttgatctt tttatgcccg
     3481 aagcaggcat ggatgccatt accctaatcg agcaacaggg catacgcgtc catttcccga
     3541 tggcgcaaag ctgctgcggt cagcccgcct attcatccgg ccatccgacc gaagcctttg
     3601 atgtcgccag agcgcaactc gaccttttcc ccgaggagtg gccgattgtc gtaccgtccg
     3661 gttcgtgcgg cggtatgatg aagcaccatt ggccgacgct gtttaaaggc agcgagtacg
     3721 aggaaagggc tgtggattgc gccggccgca tcatcgagtt tacccatttc ctgcttgcca
     3781 tcggtttcaa acccgaagac aagggcgaac cgctcaaagt cgccgttcac acttcctgcg
     3841 ccgcccgccg cgaaatgaat gtccatcttt caggctggca actgattgac ggtatggaaa
     3901 acgtcgaacg catcgtccac gaccacgaaa gcgaatgttg cggcttcggc ggcacatttt
     3961 ccgtcaaaca agccgatatt tccggcgcaa tggtaacaga caaagtcgcc gcgctgaaag
     4021 aaaccggcgc aaccgaaatc atcagcgcgg actgcggctg tatgatgaac atcggcggca
     4081 aaatcgccaa ggacgagccg gatatgccgc gtccgaaaca tatcgcatcc ttcttgttgg
     4141 aacgcaccgg aggcaaagca tgagcgcgcg tgaaaatatt ttggcaaaac tgaaaaaagc
     4201 cgatgccttg ccgatggaag agcctgcggt tttcgattat taccgcgaaa agggcgtttc
     4261 ttgggacagc gaagccgagc gtctgaaaca ttgggctgcc gcaatgtgcg cggtcaaaac
     4321 cgaaatttat tgggtgacga aaagcaattg gatgcaggtt ttccgcgaag cggcagaagg
     4381 caagggtttg aaaaacatcc tgctgccctt ggcgaccgaa cacggacaaa ttgcccgtgc
     4441 cgcattggcg gacagcaata tcgaaccgat tgccttcgag cgcgaaatcg atacttggaa
     4501 aaccgagttt ttcacgaaca tcagtgcggg cttcagcggc gcgcaatgtg gcatcgcccg
     4561 caccggcacg ctgatgctgt tttccagccc cgaagaaccg cgtactttga gtctcgttcc
     4621 gcccgtgcat ttctgcctgt tcgatacgtc caagatgtac aacgagtttc ataatgccgt
     4681 cgaaggcgaa aaactggtgg aaaacggtat gccgaccaat gtattcctga tttccggccc
     4741 gtccaaaacc gcagacatcc aactgacgct tgcttacggc gcgcacggcc cgcgcgattt
     4801 ggtcatcctc gccatcctgc ccgaccacat ttcccctgcc gatttggagg aaaacgcatg
     4861 actacgcaaa ccatcaagtt ccacatgaag ccggaaactt tcaagcaaaa cgccgcaatt
     4921 tcccttcaag acaagccttt gcgcaaaagc ctgcgtaccg cgatggatat gctgatgacc
     4981 aaacgcaaag ccgttttgac cgacgaagaa gagctgcaaa gcctgcgcga tttgtgcgaa
     5041 cacgtccgtc agcgttcatt gtctaaatta cctgccctgc tggagcagct ggaagaaaac
     5101 ctaactaagc tgggcgtgaa agtgcactgg gcagaaaccc cgaccgaagc ctgccaaatt
     5161 atccacgaca tcatcacagc caaaaacggc aagctgatgg tcaaaggcaa atcgatggtc
     5221 agcgaggaaa tcgagctgaa ccattatctt gaagcaaaag gcattaaagc ggtagaaagc
     5281 gacttgggcg agttcatcgt ccaaatggca ggtgaaaaac cgacccatat cgtgatgcct
     5341 gctatccaca aaaccaaaga acaggttagc gaactgttcc accaaaacct cggtacgccg
     5401 ctgacagacg atgtagacca actgaccggc ttcgcccgta aagcactgcg cgatatttac
     5461 agcactgccg atgtcggttt gagtggcgta aactttgccg ttgctgaaac aggtacgctg
     5521 tgtctggtgg aaaacgaggg caacggtcgc ttgagtacca ccgttccgcc cgtgcatatc
     5581 gctattaccg gcattgaaaa agtggtggcg aaattgtccg acatcccacc cttgtacagt
     5641 ctgctgccgc gctccgccat cggtcaaaac atcacaacct atttcaatat gattaccggc
     5701 ccgcgccgca gtgaagaatt agacggtccg caagaaatgc acttggttct gctcgacaac
     5761 ggccgcagcc aggcttatgc cgaagaccaa atgcgccgca ccctgcaatg tatccgttgc
     5821 ggcgcgtgta tgaaccattg cccggtttat acccgcatcg gcggcgcggc atacggcaca
     5881 acctatcccg gcccgattgg cgagattatt tcccctcacc tgttgggtct ggatgccacc
     5941 cgcgatctgc caaccgcctg taccatgtgc ggtgcgtgtg tggaagtttg tccggtacgc
     6001 atcccgatta ccgaacaaat gcagcgtttg cgcgttgaag cgcaacgttc gccgaccgaa
     6061 accgtgccgc accccatccg ggggcaaggc gcatcgcata ccttcggcga acaaatggcg
     6121 tggcgcacat tcaacggtat tttcagcggc agcaaaacct accgcgcctt cggttgggca
     6181 gccaccaagt tccgcaacct gaccccgcgc aaacagttgg gttggacgca aaaccgcgtg
     6241 ccgatgaaac cggcgaagaa aaccctgcac gaactgatgg cagaaaaaat gcgccaaaaa
     6301 gaacaggcat aaaaagttgt tcgcaaaaat gccgtctgaa acccgaaaca gggcttcaga
     6361 cggcatttgt atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     6421 aaatagtacg gcaaggcgag gtaacgccgt actggtttaa atttaagcca ctatatattc
     6481 gcggacggcg ggttttaaat ctgttccaat tccatactca aaacagcctg ttcctgtttg
     6541 gctcggaagt ctgccagttt ttgcgccagt tcgggggttt cgttggcgag catggaaacg
     6601 gcgaacaatg cggcatttgc cgcgcctgcc tcgccgatgg cgaatgtggc gacgggtacg
     6661 cctttgggca tttgtacaat cgataaaagc gaatcttcgc cgcgcaggta tttgctgggg
     6721 acgggtacgc ccaaaacggg gacggtggtc ttggcggcaa ccatgccggg caggtgcgcc
     6781 gcgccgcccg cgcccgcgat aatggctttg atgccgcgct cccgtgcggt ttcagcgtat
     6841 tggaacatta aatccggggt gcggtgtgcg gaaacgacgc gcgcctcata ttctacgccg
     6901 aactcttcaa gaaactgcgc tgcctgccgc ataacgggcc aatcgctgtt gctgcccatg
     6961 atgatgccga tttgaatcat aaatcctcct tggtgcggat ggggtaaaaa gcggaaaaat
     7021 ggaaaaacta tcgtttgcgt atggctgagg cggcgcgttt tgccgccggg ctgcccgggt
     7081 aagcctgtat cagactgcgc caagtcgccc ttgcaatgtc tttctgctgc aacctgtatt
     7141 ggcattcgcc gattttgaat atggcttcgg gcgcggttgg gctgtctttg aaacggttgg
     7201 cgtaacgccc tccgatttcg atgacggatt cgcagttccc catacgcgcc ctgctttgca
     7261 gcaacaggta catactgcgt tgcgcgatgc tgccgccgtc gcctccgtcc gcgcctttca
     7321 acagggcggc agcggcagaa aacctgccgc ttttatagtg tttgagcgcc tgattgtaga
     7381 ggttttgtgc ggtttcaacg gtatgtgcgg atgcgctgcc gccttcggta ttgaggtaat
     7441 gctctttcaa cttgcggtca tcgagttttt ggacgtatgt cctgccggaa gggtgtgttt
     7501 ttgcgtgttc cagtgctttg actttgccgt ttaaggtttc cacttcgttc gacagccgga
     7561 cgattttgcc ttccagatag tccaaacggt cttgcagagt gggaacggga tagggaatag
     7621 cgtccgaact acctgcctct gcctgcggtt cgggccgcat atcctgaata ttgcgggaaa
     7681 caggggaaga acaggcggcg gacacagaca gccaaatgat aaaaagcggt aatttggtct
     7741 tcattatttt ttcagaagca gggtcaaacc gtcgccgacg ggcagggtga tggggacgat
     7801 gcgcgggtcg ttcggcaggt tttgattaaa atctttgagg atgccgacgc tgggcggcgc
     7861 atcggaagcc gcttcgcgca tcacccttcc gttcaacaaa atattgtcga tggcaatgat
     7921 gccgccttgc cggacaagct tgaggcaacg ctcgaaatat tgcggcgtgg gcggtttgtc
     7981 tgcgtctatc agtgccaaat cgtagcttcc ggcttcaccc tgtgcaatca aatcatccaa
     8041 tgtcagcaat gcgggttgca gatgcaggct gattttatgt gccacgcctg cctcgttcca
     8101 aacctgacgc gccgtatcgg taaaggttac attgatgtcg caggcggtaa tccgcccgtg
     8161 ttttggcaac gccaatgcaa gcacggtgct gctgtatccg gtaaatacgc cgacttccag
     8221 atatttttcc gcgcgaatca gctttgccag ccaaaccaaa accgccgcct gttcgcgcgc
     8281 aatcgccatt ttgcccatac ggtgatgctc ggtcttctca cgcagccgcg tcaaaacggg
     8341 atgttcgggt tcgccgatgg cgttcaaata gttttgcagg tccggtgcga cattggacag
     8401 atgggtcgtc atttcggtgg gttcagtctt ggtaataggt ataaggtttt ttcgctactt
     8461 ttgccgcctc gaagttttcc tgttcttcgg gattgagttc gacatcccac aaaagccccc
     8521 ggttttccaa acgctgctgt tccaactcag gtttttcttc aatcagacgg ttgaggaatt
     8581 gtgtggcttc ggattggtag tgatacatct ttgcgctcca attttacggg atacggcgtg
     8641 attatactgg tattttccaa acgggataaa cggcttttat caagaataca ggcagaaaag
     8701 taaggggttt tattatagaa taaagcgttt ttgcaacgga agcctacctt atgccacgca
     8761 ttgccgccct gcccgaccat cttgtcaacc aaatcgccgc cggcgaagtg gtcgaacgcc
     8821 ccgccaacgc cttaaaagaa atcgttgaaa acagcataga cgcaggcgcg acggcgattg
     8881 atgtcgaact ggcgggcggc ggcatccgtc tcatccgcgt cagcgacaac ggcagcggta
     8941 tccatccaga cgacatcggg cttgcgctcc accgccacgc caccagcaaa atcaaaactc
     9001 taaacgattt ggaacacgtt gccagcatgg gctttcgcgg cgaaggtttg gccagtatcg
     9061 cctcagtcag ccgcctaacc ctgaccagcc gtcaagaaga cagttcgcac gcgacccaag
     9121 tcaaagcaga agacggaaaa ctcagcagcc ctacggcagc cgcccacccc gtcggcacca
     9181 ccatcgaagc cgccgaactc ttcttcaata cccctgcgcg gcgcaagttc ctcaaatccg
     9241 aaaacaccga atacgcccac tgcaccacca tgctcgaacg ccttgcactg gcgcatcctc
     9301 acattgcctt ctcactcaaa cgcgacggca aacaagtatt caaactccca acccaaagct
     9361 tgcctgaacg gattgccgcc attgtcggcg atgattttca gacggcttca ttggaaattg
     9421 acagcggcaa tggcgcattg cggctctatg gcgcaattgc caagccgact tttgccaaag
     9481 gcaaaactga caaacaatac tgcttcgtca accatcgttt tgtccgcgac aaagtgatgc
     9541 tccacgccgt caaacaggca taccgcgacg tattgcacaa cgcccttacc cctgctttcg
     9601 ttctctttct cgacctgccg cccgaagccg tggatgtcaa cgtccacccg accaaaaccg
     9661 aaatccgctt ccgcgacagt cagcaggtgc accaacttgt gttccacacg ctcaacaaag
     9721 cccttgccga cacacgcgcc aacctgaccg aaagcgtcgg caacgcaggc gaagtgttgc
     9781 atgacattac cggcgttgtc tccaccccaa tgccgtctga aaacgacagc gaaaatctgt
     9841 ttgatagcgc atccgactac ccgacaggca acaaacccga tacacgcaat gcctttggtt
     9901 catcaggcaa aaccgcgccc atgccttacc aagccgcgcg tgcgccgcaa caacacagcc
     9961 tgtccctgcg cgaaagccgc gcggcaatga atacttacgc cgagctttac aaaaaaaccg
    10021 acgacatcga ccttgagttg aaccgattag agcaggcgcg tttcggcaat atgccgtctg
    10081 aaacgcctat cccaaaaaca gatacgccgc tttcagacgg tcttccctcc caatccgaac
    10141 tgccgccgct cggtttcgcc attgcccaat tgctaggcat ctacatcctt gcccaagccg
    10201 aagacagcct gttgctcatc gatatgcacg ccgccgccga acgcgtcaac tacgaaaaaa
    10261 tgaaacgcca acgtcgggaa aacggcaaac tgcaaagcca acgcctgctt attccctcaa
    10321 cctttgccgc gtcccacgaa gaatgcgccg cccttgccga ccacgccgaa gcactggcag
    10381 gcttcgggct ggaactgtcc gatatgggcg gcaataccct tgccgtccgc acagttcccg
    10441 ccatgctcgg caaagccgac gtcgtctcac tcgccaaaga cgtattgggc gaactcgccc
    10501 aagtcggcag cagccaaacc atcgaagaac acgaaaaccg catcctcgcc actatgtcct
    10561 gccacggctc cgtccgcgcc ggccgccggc ttaccctgcc ggaaatgaac gcgctgctgc
    10621 gcgatatgga aaacacgccg cgcagcaacc aatgcaacca cggcaggccg acttgggtca
    10681 aactgacttt gaaagaattg gacgcactgt tcttgcgcgg acaataaacc gaaagtgcta
    10741 gaatacgccg cccgaagccg ccgttcagac ggcattccga cgcaccgaca gaaacatcac
    10801 gaccgaaacc aagagaaaaa catggcctat caagttctcg cccgaaaatg gcggcccaaa
    10861 accttttccg acttagtcgg tcaggaacac gtcgtcaaag ccttgcaaaa cgccttggac
    10921 gaaggcaggc tgcaccacgc ctacctgctg accggtacgc gcggcgtagg caaaaccacc
    10981 atcgcccgca tccttgccaa aagcctcaac tgcgaaaacg cgcaacacgg cgaaccttgc
    11041 ggcgtgtgcg aaagctgcac ccaaatcgat gccggacgct atgtcgacct gctcgaaatc
    11101 gacgccgcct ccaacacagg catcgacaac atccgcgaag tattggaaaa cgcccaatat
    11161 gcaccgactg ccggcaaata caaagtctat atcatcgacg aagtgcatat gctttccaaa
    11221 agcgcgttca acgctatgct caaaacgctg gaagagccgc ccgaacacgt caaattcatc
    11281 cttgccacga ccgatccgca taaagtcccc gttaccgtct tgagccgctg cctgcaattt
    11341 gtcttgcaca atatgacttc gcagcaggtt gccgaccacc tcgcccacgt cctcgacagc
    11401 gaaaaaatcg cctacgaacc ccctgccctg caacttttgg gacgcgccgc cgccggatcg
    11461 atgcgcgatg ccttgagcct gctcgaccaa gccatcgcca tgggttcggg cagcgttgcc
    11521 gaacaagacg tccgccaaat gatcggtgcg gttgacaaac aataccttta cgaactcttg
    11581 acaggcatca tcaaccaaga cggcgcagcc ctgaccgcca aagcgcagga aatggcggcg
    11641 tgtgccgtcg gctttgacaa cgccttgggc gaacttgcca tactgctgca acacctcgcc
    11701 ctgatacagg cggtgccctc cgccttggcg cacgacgacc ccgattccga tattttgcac
    11761 cgcctcgccc aaaccataag cggcgaacaa atccagcttt actaccaaat cgccgtccac
    11821 ggcaaacgcg acctgagcct cgcccccgac gaatacgccg gctttatgat gaccctgctg
    11881 cgtatgctgg cgtttgcgcc cttggcggca gcttcgtgtg atacaaacgc cgtgattgaa
    11941 aataccgaac taaaatcccc atcggcacaa accgccgaaa aggaaaccgc cgtaaaaaag
    12001 ccccaaccgc gccctgaagc agaaaccgcc caaacacccg ttcagacggc atccgcagcg
    12061 gcaatgccgt ctgaaggcaa aactgccgga cccgtttcca atcaagaaaa caacgatgtt
    12121 ccgccttggg aagacgcgcc agacgaagcc caagccgcag ccggcacggc gcaaacgtcg
    12181 gcaaaaagca ttcagacggc atccgaagcc gaaacgccgc ccgaaaacca agtttccaag
    12241 aacaaggcag ccgacaacgt aaccgatgcc cccttgtccg aagtgccgtc tgaaaacccc
    12301 attcaggcaa caccgaatga tgaagccgtt gaaacggaaa catttgcaca cgaagcccct
    12361 gcagaacctt tctacggtta cggctttccg gataatgact gccccgtaga agacggcgca
    12421 gaaatcccgc cgcccgattg ggaacacgcc gcccctgccg ataccgcagg cggaggcgcg
    12481 gacgaagaag cagaagcagg cggcatcggc ggaaacaaca cgccgtccgc cccgccgccc
    12541 gaattttcca ccgaaaactg ggcagccatc gtccggcatt tcgcccgcaa actcggcgcg
    12601 gcgcaaatgc cggcgcaaca ctccgcgtgg acggaatacc atcccgacac cggcctgatg
    12661 gttttggcga tgaccgccga agcccgcgcc accgccgaca aaaaacgtct cgacaaaatc
    12721 cgcgacaccc ttgcccaagc ctacggactg caactcaccc tgcaaaccca agactggcgc
    12781 gacgaagccg gcagggaaac ccccgcgatg caggacaagc gcgtccaagc cgaagacagg
    12841 caaaaagcac aagcattgct cgaagccgac cccgccgcac aaaaaatcct ccaagcattc
    12901 ggcgcgcaat ggcagcccga atcactggaa ttggcagcaa accggccgta aacagatata
    12961 atgccgcccg aacccttcgg acggcattgc cgtttccctc attcaatcaa aacagacagg
    13021 agtattcagt atgttcggaa aagccggatt aggcggcctg atgaaacagg cgcagcaaat
    13081 gcaggaaaat atgaaaaaag cgcaagccaa acttgccgaa accgaaatcg aaggcgaagc
    13141 aggcaacggc ctggtcaaaa tcacaatgac ctgcgcgcac gaagtacgca aaatcgacat
    13201 cagccccgat ttgattcaag aagccgccga cgacaaagaa atgctcgaag acctcatcct
    13261 cgccgccctc aaatccgccc ggggcaaagc cgaagaaacc gcaaacaaaa caatgggcgc
    13321 attcacgcaa ggtctacccc ccggagtggg cgacttcttc cgctgatccc cgaccgtcat
    13381 tcccacgcag gcgggaatct agaacgtaga atctgagaaa ccgttttact cgataaattt
    13441 ccgtgccgag gggtctggat tcccgccttc gcgggaatga cggcatcagt ttgcaagatt
    13501 cggcgtgaac ggtaaaaaca gtgagaatga taagaacgca aaaacggcaa gaatagcggg
    13561 aatcggcagg ctgaagccca ccctaccatt atttacacat ccgtaccgct taaatgccgt
    13621 ctgaaacttc gtcattcccg tgaaaacggg aatccaaccc cgtgggagca gaaacttaca
    13681 ccccgtcatt cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga atctatcgga
    13741 aaaacagaaa ccctcgaccg tcattcccgc gaacgcggga atccagtaac cgaaaaacca
    13801 cgggaatcta tcggaaaaaa cagaaaccct cgaccgtcat tcccgcgaac gcgggaatct
    13861 agaacgtaga atctgagaaa ccgttttact cgataaattt ccgtgccgac aggtttggat
    13921 tcccgccttc gcgggaatga cggcatattt tttgcatttg atataaaggg gcgtttgaat
    13981 tttgttcagc aagtgcaaag tgttgcacat aaaaaggcgc aggcttcatc cgtaaagtcg
    14041 gcgcaggata gaggcaaagc gggcgtaggt cgggctgtag caactgtatt tttcaccccg
    14101 tcgggcaaaa ataccaaaac tcaaatcaag ccgttcggag gcggctcaaa aaaacggtac
    14161 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggg cgttcgccgt
    14221 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
    14281 ttgcgtcgat ttcattggcg acttcgggat tttccttcag ccagacgcgg acgttgtctt
    14341 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
    14401 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
    14461 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
    14521 tttcgttgcc caatacctct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
    14581 ggacggaaga atagaatttc agcgcgttgc cgccggtggt ggtttcgggg ctgccgaaca
    14641 ttacgccgat cttcatccgg atttggttga tgaacacaac cagcgtgttg gtttttttga
    14701 tgtgtccggt cagtttgcgc aaagcctggc tcatcaggcg cgcctgcagt ccgacatggc
    14761 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
    14821 ctaccatatc tatgccgccc gaacggacga gtgtgtcgca gatttccaaa gcctgttcgc
    14881 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
    14941 cgggatcaaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
    15001 gggcgacggc ttcgaggcag agggtggttt tgccggagga ttcggggccg aagatttcga
    15061 cgatgcgccc gcgcggcaga ccgccgactc cgagggcgag gtctaatccg agcgatccgg
    15121 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
    15181 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
    15241 acattggggt tactccggaa caaatgcggt atgtgggatg cggcgcaaca cgggctgcgg
    15301 cgcgggatgt gtatcgtttt cccgatgtgc gggctatcgg taatgctgct tcacggggtt
    15361 gccattatcg catatttcct tgcttgccga tatgcggcag gacgcggcgg cttgtgccgg
    15421 aatggaatct ggatgccgtc taaaaggcgg ccggctttgt tataatggcg gctgtttttt
    15481 ctgtgtgtgc ctgttttatg tgttcctgcc ttgttgtcaa aaataccgtt atcggaagcg
    15541 gacgcaccaa aatcgccgtg ccgcttgtcg cccgcgatgc tgccgaactt tccgccgtac
    15601 ttgagcaaat caaaaatatg cccttcgata ttgcggagtt ccgcgccgac tttttggaat
    15661 gcgcgggcag catcggcgaa gtattgcgcc acacgcagac cgtccgcgac gcgctgcccg
    15721 acaaaccgct gctgtttacg ttcagacggc atggcgaagg cggctcgttc ccgtgttcgg
    15781 acgattatta ttttgaactg ctcgacgcgc tgatcgaaag ccgcctgccc gacatcatcg
    15841 acatcgagct gttttccggc gaaaccgccg tccggcgcgc cgtggcaaat gctcaaaaaa
    15901 acggcatcgc cgccctactc tgcaatcatg agtttcaccg cacgccgccg caagaagaaa
    15961 tcgtatgccg tctgaaacag atggaggact gcggcgcgga catctgcaaa attgcggtga
    16021 tgccgcaaag cgcggaagat gtgctgactt tgctttccgc cacgctcgaa gcgaaacggc
    16081 ttgccgccaa accgattgtt acaatgtcga tgggacagac ggggacggtc agccggcttg
    16141 ccggacaggt gttcggctca agcatcacgt tcggttcggg aacgcaaaac tccgcgccgg
    16201 ggcaaatcgg cgtatccgcc ctccgtgcgg cactcgactg tctcgaaagc ggcgcagact
    16261 gatttcagac ggcatcaaaa catgatgaaa ctcaatcccc aacagctcga agccgtccgt
    16321 tacctcggcg gccccctgct cgtccttgcc ggtgcaggca gcggcaaaac cggcgtaatt
    16381 actcaaaaaa ttaagcattt gattgtcaat gtcggctatc tgccgcatac cgtcgccgca
    16441 attaccttta ccaacaaagc cgctgcggaa atgcaggagc gcgtcgccga aatgctgccc
    16501 aagtcgcaaa cgcgcgggct gacaatttgc acgttccatt ctttgggcat gaagattttg
    16561 cgcgaagagg cgaaccatat cggttacaaa aaaaactttt ccatcctcga ttccaccgac
    16621 agcgcgaaaa tcatcggcga acttttgggc ggtacgggca aagaagccgt attcaaggcg
    16681 cagcaccaga tttccttgtg gaaaaacgat ttaaaaacgc ctgaagatgt cgttcagacg
    16741 gcctctaatg cgtgggaaca acaaaccgca cgcgtgtatg cgagctatca ggaaacctta
    16801 caaagctatc aggcagtgga cttcgatgac ttaatccgcc tgcctgccgt gctgttgcag
    16861 caaaacagcg aagtgcgcaa caaatggcag cggcggctgc gttatctgct ggttgacgaa
    16921 tgccaagata cgaatacctg ccaatttgcg ctgatgaaac tcttgaccgg tgcggaaggt
    16981 atgtttaccg ccgtcggcga cgacgaccag tccatctacg cgtggcgcgg tgcgaatatg
    17041 gaaaacctgc gcaaaatgca ggaaaactat ccgcagatga aggtcgtcaa actggagcaa
    17101 aactaccgct ccaccgcgcg gattcttaaa atcgccaaca aagtcatcga aaacaacccc
    17161 aagctgttta ccaaaaaact ttggtcgcaa ttgggcgaag gcgagccggt caaagtcgtt
    17221 gcctgccaaa acgagcaaca cgaagccgac tgggtcgtca gccaaatcat caaacaaaaa
    17281 ctcatcggcg gcgacaaaac ccaatacgcc gatttcgccg tgttataccg cggaaagcat
    17341 caggcgagga ttttcgagga agcattgcgc ggcgtgcgca tcccctaccg gctctccggc
    17401 ggacaaagct ttttcgacaa agccgaaatc aaagacgtgt tgtcttatgt gcggctgctt
    17461 gccaacccca acgacgatcc cgcctttctg cgcgccgtaa ccacgcccaa acgcggcatc
    17521 ggcgatgtaa cgctgggcaa gctcaacgcc tacgcgcacg aacacgaatg cagcctgtat
    17581 gaagccgcgc aaaccgaaga agcccttgcc acgctgaaca ataccaaccg ccaacacctg
    17641 caagccttta tggatatgtt cggcagctac cgcgccaaag ccgaaacagg cgaagcaggc
    17701 gagttcatca acagcctgct cgaagaaatc gactatgaaa accacctgct tgccagtgaa
    17761 gaaggcaaag ctggcgaaat caaatggcgc aacgtcggcg atttggtatc atggtttgcg
    17821 cgaaaaggcg gggaagacgg caaaaacatc atcgaactcg cccaaaccgt cgccttgatg
    17881 acgcttttgg aaggaaaaga cgaagaagaa accgatgccg tctcgctatc cacgctacac
    17941 gccgccaaag gtttggagta tccctatgtt ttccttgtcg gttgcgaaga aggtgttttg
    18001 ccgcacaacg acagcatcga agaaggcaac gtcgaagaag aacgccgcct gatgtacgtc
    18061 ggcatcaccc gcgccaaacg ccagcttacg ctgacccact gcgtcaaacg caaaaaacaa
    18121 ggcatatggc agttccccga acccagccga ttcatagacg aaatgccgca ggaagatttg
    18181 aaaatcctgg ggcgcaaagg cggcgaaccg attgtcagca aagaagaagg cagacgcaac
    18241 cttgccgata taatcggaag gctcgacagc atgaaaaaag gcggtccgga ggattaaacc
    18301 aagataggga aacgaagccg gagacaatgt atagtg
//
LOCUS       DAWGJV010000029        17824 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWGJV010000029 DAWGJV010000000
VERSION     DAWGJV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.029"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(131..466)
                     /locus_tag="WHQ94_000694"
     CDS             complement(131..466)
                     /locus_tag="WHQ94_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ2128015.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            595..1500
                     /gene="mtrA"
                     /locus_tag="WHQ94_000695"
     CDS             595..1500
                     /gene="mtrA"
                     /locus_tag="WHQ94_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ2128016.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1562..2050)
                     /locus_tag="WHQ94_000696"
     CDS             complement(1562..2050)
                     /locus_tag="WHQ94_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ2128017.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2197..3099
                     /locus_tag="WHQ94_000697"
     CDS             2197..3099
                     /locus_tag="WHQ94_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2128018.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3140..4270)
                     /locus_tag="WHQ94_000698"
     CDS             complement(3140..4270)
                     /locus_tag="WHQ94_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2128019.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIARRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4475..7099
                     /gene="alaS"
                     /locus_tag="WHQ94_000699"
     CDS             4475..7099
                     /gene="alaS"
                     /locus_tag="WHQ94_000699"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ2128020.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     TDLVKEMGDAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(7188..8210)
                     /locus_tag="WHQ94_000700"
     CDS             complement(7188..8210)
                     /locus_tag="WHQ94_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2128021.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8264..9415)
                     /locus_tag="WHQ94_000701"
     CDS             complement(8264..9415)
                     /locus_tag="WHQ94_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ2128022.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQYLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
     gene            10038..10268
                     /locus_tag="WHQ94_000702"
     CDS             10038..10268
                     /locus_tag="WHQ94_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128023.1"
                     /translation="MFKTLKSIWQGLTQKGKIFPYQLAFTLLIPLRNWALSPQQITQR
                     LHFVPHHRICCGAGYFSCVGCASAHRFLIFAC"
     gene            10509..11453
                     /gene="tehA"
                     /locus_tag="WHQ94_000703"
     CDS             10509..11453
                     /gene="tehA"
                     /locus_tag="WHQ94_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ2128024.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYAGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GILSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(11527..12210)
                     /locus_tag="WHQ94_000704"
     CDS             complement(11527..12210)
                     /locus_tag="WHQ94_000704"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ2128025.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSGQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            12506..14809
                     /gene="parC"
                     /locus_tag="WHQ94_000705"
     CDS             12506..14809
                     /gene="parC"
                     /locus_tag="WHQ94_000705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ2128026.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            14830..16347
                     /locus_tag="WHQ94_000706"
     CDS             14830..16347
                     /locus_tag="WHQ94_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ2128027.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASVTFILVAASYLSAIFTSLSVRYIDSAGRLARDNHLAYRRIKGLSQTVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTALFDPITVLWDKASPHTFEYVLGTSGLNA
                     GIRAVPVNKGPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            16399..16866
                     /locus_tag="WHQ94_000707"
     CDS             16399..16866
                     /locus_tag="WHQ94_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ2128028.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQADKRLGISFRSMRYRMERLNIG"
     gene            16970..17722
                     /locus_tag="WHQ94_000708"
     CDS             16970..17722
                     /locus_tag="WHQ94_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2128029.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         4260 a         5003 c         4379 g         4182 t
ORIGIN      
        1 cgcttacccc ttgtttctca aatgccgtct gaaaaaacgg ctgcatatgt tgtctacaaa
       61 atatcgggaa gcataacaga caaaatgccg tctgaaatat tttcagacgg catttcttat
      121 ccgaaacgga ttatttttgc gtttcaaccg cttccaatgc acgcagggcg taagtgtaag
      181 cggcaccggc attcagggcg atggcggttg ccaatgcgcc tgcgatttcg ctgtcggtcg
      241 caccggcttt ggcggcagcg gcggcgtgaa cgctgatgca gctctcgcaa cgagtggtaa
      301 tggcaacggc gatggcaatc agttcgcgcg ttttggcatc aagtgcttcg gcggcggcgg
      361 cctgttccaa tgcgccgtag gcctgcagca ttttaggatg cgccttaccc agctcgccga
      421 acgatttttt aaccaatgcg gtatgttctt tccaatcttt aaacattttc ttttcctttc
      481 tcttgcgttt aaccctgata cgcgcttgcg tatctgtttt cgatgtgcgt attattgcaa
      541 ttattcagtt gtgtttctcg tttaatcatc tcattttatg gttcaaaaag atttatggac
      601 attctggaca aactggtcga tttcgcccaa ttgacgggca gtgtggatgt gcagtgcctt
      661 ttgggcggac aatggtcggt acggcatgaa accttgcaac gcgaaggact ggtacacatt
      721 gttacatcgg gcagcggcta tctctgcatc gacggcgaaa cttccccgcg tccggtcagt
      781 acaggggata ttgtattttt cccgcgcggc ttgggtcatg tgttgagcca cgacggaaaa
      841 tgcggagaaa gtttacaacc ggatatgcgg cagcacggtg cgtttacggt caaacagtgc
      901 ggcaacggac aggatatgag cctgttttgc gcccgtttcc gctacgacac ccacgccgat
      961 ttgatgaacg ggctgcctga aaccgttttt ctgaacattg cccatccgag tttacagtat
     1021 gtggtttcaa tgctgcaact ggaaagcaaa aaacctttga cggggacggt ttccatggtc
     1081 aacgcattgt cgtccgtcct gctggtgctt atcctgcgcg cctatctcga acaggataag
     1141 gatgtcgaac tctcgggcgt attgaaaggt tggcaggaca aacgtttggg acatttaatc
     1201 caaaaggtga tagacaaacc ggaagacgaa tggaatgtcg acaaaatggt ggcggctgcc
     1261 aatatgtcgc gcgcgcaact gatgcgccgt ttcaaaagcc gggtcggact cagcccgcac
     1321 gcctttgtga accatatccg cctgcaaaaa ggcgcgttgc tgctgaaaaa aaacccggat
     1381 tcggttttgt cggtcgcact gtcggtaggc tttcagtcgg aaacgcactt cggcaaggcg
     1441 ttcaaacggc aatatcacgt ttcgccgggt caataccgga aagaaggcgg gcaaaaataa
     1501 atcggggctt caaacgcaaa tgccgtctga aaaggctttc atacagcatt tgcgtaccgc
     1561 gtcatttcaa gggctgcatc ttcatcactt ccatcaaaaa gttggtaaat gcggggttgt
     1621 tgggtttgac atccatattt ttccaacgct gctgccagcc gcgcaaggca ttctggatat
     1681 acagcttgga ctgttccgta ttgattgcgc cccgctggct gtctatcgcc gaacgcaggt
     1741 agatttcata catactgtca tcgacggcat tgcgtccgac caggcgtttt ctgaagttgt
     1801 tcagatattg cgccgcctga accttggtca ttttaccgat acccacctga tagcccaagc
     1861 gcgtcgcttc atcgctgatt ttggcaacat ccgtccaatg cgaagaggca aggcggaaac
     1921 cttttgcagg tgcttccgtt ttgacggtat tgataggatt cacggggatt tccgtcaatg
     1981 tgggcacata aatagactgg cagccggaaa gaactgccac aatggaaaga gggataaggt
     2041 attttttcat gcccccatta taatcaagtt tgccttgaga aaacaaattg ttcggcaaga
     2101 aaaataaaat ttcggcatca gaagcaggca aaaacacatt ccacaagcct tgccgcaagg
     2161 tttacaatcc gaccgtcctt atcgcaacga ccgtttatgg ataccgcaaa aaaagacatt
     2221 ttaggatcgg gctggatgct ggtggcggcg gcctgcttta ccattatgaa cgtattgatt
     2281 aaagaggcat cggcaaaatt tgccctcggc agcggcgaat tggtcttttg gcgcatgctg
     2341 ttttcaaccg ttgcgctcgg ggctgccgcc gtattgcgtc gggacacctt ccgcacgccc
     2401 cattggaaaa accacttaaa ccgcagtatg gtcgggacgg gggcgatgct gctgctgttc
     2461 tacgcggtaa cgcatctgcc tttggccacc ggcgttaccc tgagttacac ctcgtcgatt
     2521 tttttggcgg tattttcctt cctgattttg aaagaacgga tttccgttta cacgcaggcg
     2581 gtgctgctcc ttggttttgc cggcgtggta ttgctgctta atccctcgtt ccgcagcggt
     2641 caggaaacgg cggcactcgc cgggctggcg ggcggcgcga tgtccggctg ggcgtatttg
     2701 aaagtgcgcg aactgtcttt ggcgggcgaa cccggctggc gcgtcgtgtt ttacctttcc
     2761 gcgacaggtg tggcgatgtc gtcggtttgg gcgacgctga ccggctggca caccctgtcc
     2821 tttccatcgg cagtttatct gtcgtgcatc ggcgtgtccg cgctgattgc ccaactgtcg
     2881 atgacgcgcg cctacaaagt cggcgacaaa ttcacggttg cctcgctttc ctatatgacc
     2941 gtcgtttttt ccgccctgtc tgccgcattt tttctgggcg aagagctttt ctggcaggaa
     3001 atactcggta tgtgcatcat catcctcagc ggtattttga gcagcatccg ccccactgcc
     3061 ttcaaacagc ggctgcaatc cctgttccgc caaagataaa aaatgccgtc cgaacatcct
     3121 tcagacggca tatcgggctt tatttccccg ccttcacatc ctgccactgg cgcaccataa
     3181 acttcaatgc cgccggctgg ataggcacca tgataaagct gtttttcaaa tcctcctcgg
     3241 ttgggaaaat cgtattgtcg tttttaaatt cgtcttccat cagctcacgc gccggcttgc
     3301 tcgaaggcgc ataagtaacg aaattgccgt ttttcgccga cacttccggg tcgaggaagt
     3361 cgttgatgta tttgtgcgcg ttggcgacgt ttttcgcatc tttcggaatc acgaaagaat
     3421 ccacccaaat ccccacgccc tctttgggca tcatcacgcg gattttttcc ttgccgcccg
     3481 cttcttcggc acgccgtctg gcgatgttca aatcgccgcc gaaaccgatt gttacgcagg
     3541 tatcgccgcg cgccaaatca tcgataaagc cggacgaagt aaagcgtttg atattggggc
     3601 ggtttttctt gagcagggcg gttgcctccc tgatgtcttc cgtattgctg ctgttcgggt
     3661 ttttacccaa atagttcaac accataggat aaatttccgc cgcgctgtct aaataactga
     3721 taccgcattg tttgagtttg gacgtgtatt cggggtcgaa caccaaatcc cactggttgt
     3781 cgggcagctt gtccgtaccc aaagcctttt tcacgcgttc ggtattgatg gcgaaggtat
     3841 ttgtccccca ataaaacggc acggcgtatt cgtggccggg atcgaccccg tccatcagcc
     3901 tcatcatttc agggttgaga tgtttataat tgggaatcag tgacttatcg attttctgat
     3961 aagcccccgc cttaatctgc ctgcccacaa acgcattgga cggcgcgaca atgtcgtaac
     4021 cggatttgcc ggtcagcacc ttgctttcca gtgtttcgtc gctgtcgtac acatcataag
     4081 taaccttgat gccgtttttc ttttcaaaat cggcaacggt ttccggatcg acgtattccg
     4141 accagttgta aattttcaat acgttttggt tttccgccgg tgccggtttt tcggcaggcg
     4201 gtttgtccga accgccgcac gctgcaagca gcaaagcagt caggacggcg agtggcagat
     4261 gtttggccat tatcattcct tgcatatcgg gttggagaaa gcggtcatta tagccgatat
     4321 tggcaacagg gcttcagacg gcattcaaaa tcccgccaca cccttccgaa aaccgccgct
     4381 tccatagcta gaaacaggga tttgcggtaa gataccgccg ttcgttttcc ctgcttttac
     4441 catgacaaga catttgagag acattgaaaa aattatgaaa acctccgaac tgcgccaaaa
     4501 attcctaaaa ttttttgaaa ccaaaggcca caccgtcgtc cgctcctcca gcctcgtgcc
     4561 gcacgacgac ccgaccctgc tgtttaccaa cgcgggcatg aaccaattta aagacgtatt
     4621 tttaggtttc gacaaacgcc cgtacagccg cgccaccacc gcgcaaaaat gcgtacgcgc
     4681 aggcggcaaa cacaacgact tggaaaacgt cggctacacc gcccgccacc acaccttctt
     4741 tgaaatgatg ggcaacttct ccttcggcga ctacttcaaa cgcgacgcca tccacttcgc
     4801 ttgggaattt ctgacttccc ccgaatggct caacatccct aaagacaaac tgttggcgac
     4861 cgtttacgcg gaagacgacg aagcctacaa catctggttg aacgaaatcg gtatgccgtc
     4921 cgagcgcatc gtccgcatcg gcgacaacaa aggcgcgaaa tacgcatccg acaacttctg
     4981 gcaaatgggc gacaccggcc cttgcggccc ctgctccgaa attttctacg accacggcga
     5041 agaaatctgg ggcggcattc ccggcagccc cgaagaagac ggcgaccgct ggatcgaaat
     5101 ttggaactgc gtatttatgc agttcaaccg cgacgaacaa ggcaatatga atccgctgcc
     5161 caagccgtcc gtcgataccg gcatgggttt ggagcgcatg gcggcagtaa tgcaacacgt
     5221 ccacagcaac tacgaaatcg acttgttcca agacctgctc aaagccgttg cccgcgaaac
     5281 cggcgcagct ttcagcatgg atgaacccag cctgaaagtc atcgccgacc acatccgctc
     5341 ctgctccttc ctgattgcag acggcgtatt gccgtccaac gaaggccgcg gctacgtatt
     5401 gcgccgcatt atccgccgcg ccgtgcgcca cggttacaaa ctgggtcaaa gcaaaccgtt
     5461 cttccacaaa ctcgttaccg atttggtcaa agagatgggc gatgcctacc ctgaattgaa
     5521 agaaaaacag gcgcaaatcg aagaagcgtt gaaaaacgaa gaaagccgtt ttgcccaaac
     5581 tttggaaacc ggtatggctt tgttggaaaa tgcactggcc aaaggcggca aaacactcga
     5641 cggcgaaatc atcttcaaac tctacgatac ttacggtttc ccatacgacc tgaccgccga
     5701 catctgccgt gaacgcaata ttgaactgga cgaagcaggc ttcgagcgcg aaatggaagc
     5761 ccaacgcgca cgcgcacgcg ccgcccaaag cttcaaagcc aacgcccaac tgccttatga
     5821 cggtcaagac accgagttta aaggttatag cgaacgccaa accgaatcca aagtcctcgc
     5881 cctctacaaa gacggcgagc aagtcaacga attgaacgaa ggcgacagcg gcgcagtcgt
     5941 tatcgacttt accccgttct atgcagaatc cggcggccaa gtcggcgacg tcggctatat
     6001 cttctcaggc gaaaaccgct ttgaagtacg cgatacccaa aaaatcaaag cggccgtatt
     6061 cggtcaattc ggcgtacaaa cttcaggccg tctgaaagtc ggcgacagcg ttaccgccaa
     6121 agtggatgac gaaatccgca atgccaatat gcgcaaccac agcgcaaccc acttgatgca
     6181 caaagccctg cgcgatgtat tgggcgaaca cgttgaacaa aaaggctcgt tggttaccgc
     6241 cgaatccacc cgtttcgaca tttcccatcc tcaagcggta actgccgaag aaatcgccga
     6301 agtagaacgc cgcgtcaacg aagccatttt ggcgaacgtt gccgtcaacg ccgccattat
     6361 gagcatggaa gacgcccaaa aaaccggcgc gatgatgctt ttcggcgaaa aatacggcga
     6421 cgaagtacgc gtactgcaaa tgggcggttt ctctaccgaa ttgtgcggcg gcacacacgt
     6481 ttcacgcacc ggcgacatcg gtctcttcaa aatcatcagc gaaggcggta ttgccgcagg
     6541 cgtgcgccgt atcgaagcca tcaccggcct gaacgcgctc aaatgggcgc aagagcaaga
     6601 acgcttggtt aaagacatta ttgccgaaac caaagcccaa accgaaaaag acgtactggc
     6661 gaaaatccaa gcaggcgcgg cacacgccaa agcattggaa aaagaattgg cacgcgccaa
     6721 agccgaactc gccgtccacg caggcgccaa actcttggac gatgcaaaag acttgggcgc
     6781 agccaaactc gttgctgccc aaatcgaagc cgacgcagcc gccctgcgcg aaatcgtaac
     6841 cgacctgacc ggaaaatccg acaacgccgt gattcttttg gcggcagtaa acgacggcaa
     6901 agtctccctg tgcgcaggcg tatccaaagc cctgaccgga aaagtgaaag caggcgatct
     6961 ggttaaattt gcagccgaac aagtcggcgg caaaggcggc ggcagaccag atttggcgca
     7021 agccggcggc acggatgccg acaaattgcc cgccgtgttg gatagcgtga aagactgggt
     7081 cggcgcgaag ctggtttgat gtgggaaagg cagcctgaaa gggattcagg ctgccttttg
     7141 tgttaaagag gccgtctgaa agatattttc aaacggcctc tgtcagttta tttggcttta
     7201 tacctaccat tgaatagcga ttcagaaggt atccagccat ctgccaaatc atccaacatc
     7261 ttaatccaat actctaggcg tttttctgtc gggacgcttt ctccccaata ttcgacaatt
     7321 agagcatcat ccatttccaa agcagctttt tcaatttcat gctcaatcaa ttttaaagat
     7381 ttttctacat agtttccgcc acggtagtct tccgccgagt tggcaacgct gcccgcactg
     7441 aataagtccc gcgcctgatg acaaagcaca ccgcgatagg taatcagata caggacggca
     7501 ttatcgtatt tgtgcttcgc cctgcctgag cagacaatac ggggggagac taatgctgct
     7561 tcagctcgga ggcggatttt ttcttcctgc ggcagggaag tgtaaaggcg cacttccaaa
     7621 ggcagatcat ggctgtctac tgcgtcaatg tcttctatcc accataaccg ctcgccttct
     7681 ttcaacctgt tacgggcata ttttcggata tattccattt tttcatgcat atccaaatcg
     7741 gcaaattcag cataggaaat accgaaaccc tcaaacagag acggtcgatc agattctatt
     7801 tctacctcaa agcgcggaac atgcgatgtg cggacatgaa ttacgctatc ttcgtaaagt
     7861 tgccatttca catcgggaat gccccccatt tttccgaata ccacatagat atgctgtaca
     7921 ccgtcagctt tgtttttctc ttggatactg tttgctacgc tgcgccaagt atcttttagc
     7981 gtgaatttga cttctacacc atattcccct attggaacat cggggaaaat ttgcggcaca
     8041 ggttcaggat tgataactat gtcagcataa gcgggaattt ttgcaatgca cagcgcacga
     8101 actctgtttt caaaaatatc ttgggatttg aaaggtgcgt tttttgcctc ttgcctcaat
     8161 acggtgcaaa cctcgtctaa aaacttctca aactcctttt ttgtcagcat aatgcattac
     8221 cacttaaaaa tataaagtag taattttatc ttatttaaca gggttattta agtgccttac
     8281 taacggcggc ggcgatgtgc caccctaaaa caggagggac ggcgttgcca atttgccgtt
     8341 ctgtggcacg caattttgcc tggaaaataa aattgtcggg aaacgattgg atgcgcgccg
     8401 cctcacgcat ggagatgcgg cggggtaatt cgtaatgcca ctgtatgttt ccatggcatt
     8461 ctgccctaat agtgtagccc gccctttcgg catccaattt tcgactacct tgatcggggc
     8521 ttttttctgc tctactccaa atatggttgg caatttcgtc ttctactttg tcgaccaaat
     8581 cgcctatagc ctgtctagct gttacatagc tgttgctggt atttgttttt tcaatctgta
     8641 tttcgggaat ttcaggtagc cttccaacga tgataactcg ctcccgagtc tgtggaacac
     8701 cgtaatcggc tgcattcagc aagtagtaat ctacctgata acccaaattg cgaaaatctg
     8761 aaagcactgt ttccaaatct ttccgatgat gcttcatcag tagccctttg acattttcag
     8821 caacaaatat tttggggcga acgcgttcaa cagcagacaa catggcttta tacagcccgc
     8881 tgcggctgcc tgaaatacct gcacctttac cattaataga aatatcctga caaggaaagc
     8941 caccgataat gatgtctgcg ctagtgggta tttcatccaa atactgccaa atatcaccat
     9001 gtacaatgtg atttcctaag ttgtggcggt aggttctaac agcgtgttcg tcgatttcgt
     9061 tcgcccaaat aatatcaaaa tcatgtttgt cataagtacg gtttaaaaac tcaaagcccc
     9121 ccagaaaacc caaatccatt ccgccacaac cgctaaataa agaaataatt ttatattttt
     9181 tttcgcggtc ttcttgcttt tcctgtttgg tcattaactc catacattta agataaaaag
     9241 catgggaaaa agtacctcta tttactttgt tacaaattgc atttgccgtt tcactttccc
     9301 cttgctccaa gaataattct gataattgta taaagccaat tccatgctca tgcattttgg
     9361 cttttatggc atctccagct aacttgttcc aattttttcg tgcaaattcg gtcatggtta
     9421 atctttcagt cattcataaa tgacattatt ttagcgtttt ttatagttta agccaagatt
     9481 taaaatctac ctttaggttt acaggccgtc tgaaaacaaa aatctcgttt tcagacgacc
     9541 tttttccgct aaaatccccc taccgtccaa cccgaaacaa ggagaaccat catgcaagtt
     9601 cctacttccg ccatcatcaa cggcgaattt gccgacaaat acggcaaacg cggcagcaag
     9661 ccgtagccag cacccctgtc aaccgcgtgc gtggctagac cacacaccct accactgcgg
     9721 gcgacgcaaa ccttaagatt gaggtgtagg gtgtgtgcgg tacgcacgca cgcggttttt
     9781 gttactaccg aatgattcca aaccatgccg taatccagtc agcagcaaac gctgcttggg
     9841 tcatgtacca acaaaactca atatcaaagg ccgtctgaag aataattttc agacggcttc
     9901 tctctatcaa aagcagcctg cacattcaaa aatcgaagtg caggctgctt tttgtgatga
     9961 ttggaaaaaa cacacactat ttttatactg tatattgtgc aggtacaaag taaaacgaat
    10021 tacccaaaga aattcccatg tttaagacgc tcaaaagcat ttggcaggga cttacgcaaa
    10081 aaggcaaaat cttcccgtac caactggcat tcacgctgct gattcctttg cgtaactggg
    10141 cgctttcacc gcaacaaatc acgcaacggc tgcactttgt tccgcaccac cgaatttgct
    10201 gcggggcggg ttatttcagt tgcgtaggat gtgcttcagc ccaccgtttc ctcatttttg
    10261 cttgttaatt ggtgggctga agcccacact acggttaata cggttactga ttcgtttccc
    10321 cctgcatccg aatcacgccg cacaccgcaa aaaacgcgcg cccgatgcgc aaccgcttcc
    10381 gacatctggc gcaatgagac aaaacggcaa tatgccgata cggatacgtc atcctccgcc
    10441 tgcctgtttt ttatatcata ctcaatttta tctgtgcgtt acgacggcat gccgtctgaa
    10501 gggtgtttat gtctgcatct aagaaatttc cgattccttt gagctatttc agcatcgcgc
    10561 tgggcttgtt tgccttgggg ctgtcgtggc gttacggcgc gtctgtcggg ctgctgcccg
    10621 ccttggccgc cgaatcgctg cttgcggcgg cttcggtcgt ctggctcttg ctggtggcgg
    10681 catacctgat caaaatgttt gcgtaccgaa acgatttttt gtctgatttg cgcgacttgg
    10741 tgcaatgctg cttcatcagc gcgattccga ttaccgctat gctggaggga ctcgcgctga
    10801 agccctatca ggcaggcgcg gcggcagtcc tgatttatgc cggcgttgcc ggacagttgg
    10861 cgttttcgat gtatcgggcg gccggtctgt ggcgcggcct gcattccttg gaggcgacga
    10921 cgccgattat ttatctgcct acggttgcga caaactttgt cagcgcgtca tctctggccg
    10981 cgttggggca tcatgattat gcagctttgt ttttcggcgc gggtatgttt tcctggctga
    11041 gcttggaagc ctccatcttg ggcaggctgc gcacggcggc accggtcggc acggcggcgc
    11101 gcggcgtggt cggcatccag cttgcgcccg cctttgtcgg ctgcggcgcg tattttgccg
    11161 tcggcggtaa agtcgacggt tttgcgttgg cattaatcgg ctacggctgc ctgcagcttt
    11221 tgttcttgct gcgcctgacc cgctggtttt gggaaggcgg ttttacgatg agcttttggg
    11281 gattttcatt cggtttcgcg gcaatggcag gatgcggtct gcatctggcg gcttctggca
    11341 tattgtcggg cttggggctg acgcttgcca ccgccggatc ggcaggcgtg gcgctgctgc
    11401 ttgtcggtac gctgcaccgg atagcgacgg ggcgtttctt ggtacgcggc tgatgcgttt
    11461 tgccgccttg tcaaaaatgc cgtctgaacg ctcaagcctt cagacggcat tttttatttc
    11521 acacccttac aggtagaatt tttcgatgac tttcaaattg tcgtccaatt tgtacaccaa
    11581 cggctgaccg gtcgggattt ccaagcccat gatgtctttg tcggaaatgc cctcgatgtg
    11641 ttttgccagc gcgcgcaggg agttgccgtg cgccgccacc aagacgcgtt tgccgctcaa
    11701 aatcgcgggg gcgatttggt cttcccaaaa cggcaatacg cgctccagcg ttactttcag
    11761 gttttcgccg tcgggtacga catcggcagg cagatgggca tagcggcggt ctttgtgtgc
    11821 ggaaaactca tcgtctttgt ccaaaagcgg cggcagggtg tcgtagctgc gccgccagat
    11881 gcggacttgc tcgtcgccgt attgttcggc ggtttgtttt ttgtccaaac cttgcagtcg
    11941 gccgtagtgg cgttcgttca gccgccatgt tttgatttgc ggtacgaaca gttggtcgga
    12001 ttcttccaaa acgatgttgc aggtcttaat cgcgcgggtc aggacggatg tgaaggcgat
    12061 gtcgaactca tagccgtttt ctttcagttt tttaccggcg gcggcggcct cggcaagccc
    12121 ctgcccgctc agcttcacgt cgcgccagcc tgtaaacagg tttttcgcgt tccattcgct
    12181 ttgtccgtgg cggataaata ccaattccat atcgtctcca atgtgtgaaa gtgggaaagc
    12241 cttatttata acatattttc acattcccgt atttgattca gattcagaca cgcgcccact
    12301 atggtttgcc gttttgattt acaataatgc cctttgcttt acattccgca tacacaatgt
    12361 ttcaacacac aggacgacac ataaagcacc gccctatgtg ttgccctgat ttggaagggg
    12421 ttgccccctc ccaaataaag tctgattcta ccgccccgaa ggacagatgt ccaagtggcg
    12481 ggggttcaac cgaaaaggaa atacgataaa tatgcaaccg cacgctccac ataccgattc
    12541 caatacgctg atgctcggcc gatacgccga acgcgcctat ctcgaatacg ccatgagcgt
    12601 ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccaa aagcccgttc agcggcgcat
    12661 tttgtttgcg atgcgcgata tgggtttgac ggcgggagcg aagccggtga aatcggcgcg
    12721 cgtggtcggc gagattttgg gtaaatacca cccgcacggc gacagttccg cctatgaggc
    12781 gatggtgcgg atggcgcagg attttacctt gcgctatccc ttaatcgacg gcatcggcaa
    12841 cttcggttcg cgtgacggcg acggggcggc ggcgatgcgt tacaccgaag cgcggctgac
    12901 accgattgcg gaattgctgt tgtccgaaat caatcagggg acggtggatt tcgtgccgaa
    12961 ctacgacggc gcgtttgacg aaccgctgca cctgcccgcc cgcctgccta tggtcttgct
    13021 caacggtgcg tcaggcattg cggtgggtat ggcgaccgaa atcccatcac acaatttgaa
    13081 tgaagtcaca caggcggcga ttgcactgtt gaagaagccg acgctggaaa ccgccgacct
    13141 gatgcaatac atccctgctc ccgattttgc cggaggcggt caaatcatca cgccggcgga
    13201 cgaattgcgc cgtatttacg aaaccggcaa gggcagcgtg cgcgtgcgtg cgcgttacga
    13261 aatcgagaag ctggcgcgcg gacaatggcg cgtcatcgta accgagctgc cgccgaacgc
    13321 caattccgcc aaaatccttg ctgaaatcga agaacagacc aacccgaaac cgaaagcagg
    13381 caaaaagcag ctcaaccaag accagctcaa tactaaaaag ctgatgctgg atttaatcga
    13441 ccgcgtgcgc gacgagtcgg acggcgaaca tcccgtgcgc cttgtatttg aaccaaaatc
    13501 cagccgcatc gataccgata ccttcatcaa cacgctgatg gcgcaaactt cgctggaagg
    13561 caatgtgtcc atgaacttgg tgatgatggg gctggacaac cgccccgcgc agaaaaacct
    13621 gaaaacgatt ttgcaggaat ggttggattt ccgcaccgtt accgtaacac gccgtctgaa
    13681 attccgtttg aaccaagtgg aaaaacggct gcacatcctc gaaggccgtc tgaaagtctt
    13741 tctgcacatc gacgaagtga ttaaagtcat ccgcgaatca gatgacccga aagccgattt
    13801 gatggcggcg ttcgggctga ccgaaatcca agccgaagac attttggaaa tccgcctgcg
    13861 ccagttggca cgtttggaag gtttcaaact cgaaaaagaa ttgaacgaat tgcgcgaaga
    13921 acaaggccgt ctgaacatcc ttttgggcga tgaaaacgaa aagcgcaagc tgattatcaa
    13981 agagatgcag gcggatatga aacaatacgg cgatgcacgc cgcacgctgg tggaagaggc
    14041 cggacgcgcc gtgctgacac aaaccaccgc cgacgaaccc atcacactga ttctgtcgga
    14101 aaaaggctgg atacgcagcc gtgccggaca caatctcgat ttgagccaaa ccgcgttcaa
    14161 agaaggcgac cgcctcaaac aaacccttga aggcagaacg gttttacccg tcgtcatcct
    14221 cgattcattg ggcagaacct atacactcga tgccgccgaa attcccggcg gacgcggcga
    14281 cggcgtgccg gtttcatcct tgatcgagtt gcaaaacggc gcgaaacccg ttgcgatgtt
    14341 gacaggattg ccggaacaac attatttatt atcaagcagc ggcggctacg gcttcatcgc
    14401 caagttggct gatatggtcg gacgcgtaaa agcgggcaaa gtggtgatga ccacagacag
    14461 tggcgaaacc gtcctgccgc cgattgccgt ctatgcctcc tcgttcatca accccgactg
    14521 caaaatcatt gccgccacca gtcaaaaccg cgccctcgcc ttccccatcg gcgaattgaa
    14581 aattatggcg aaaggcaaag ggctgcaaat catcggatta aacgccggcg aatcgctgac
    14641 gcataccgcc gtttcttccg agccggaaat cctgattgaa agcgaaggca ggcgcggcgc
    14701 ggcgcacaaa gaccgcatcc ccgtctccct gcttgaggca aaacgcggca aaaaaggcaa
    14761 actattaccc atatcgggca gcctgaaaca gctttcttcc cccaaataaa cccgattcca
    14821 cacataatta tggtgatttc caacccccgc gaacttgaaa aactcaaaga ccggattccc
    14881 aatctgatca acatcatccg cgtcgccatc gtttttccgc tgatgattat gcacatcctc
    14941 gggctggaaa ccggcagccg tgcgaacctg cacgcttcgt ggacggcgtg ggcgttttat
    15001 gtttggctcg ccattgcctg ctggctgatt ttcttttcca tcatccatcc gcattggcaa
    15061 tggcagtcgc tgaaaatgcc gcgtttcagc gcggtggcgg acatcacgat gatcggcgtg
    15121 ctgacctacc tgtttggcgg catcgattcc ggcttcggca tcctgatcct gcccttcgtc
    15181 gtctgctcct gcctgctcag ctacgggcgt taccccctgc tctattccag ctacgccgcc
    15241 atcctgctga tattcaacgc cattgccgac ggcgatatcg gcaaataccc gctcatatcg
    15301 gatgcccgaa ccgcctcggt aaccttcatc cttgtcgccg cctcctatct ttccgccatc
    15361 ttcacctcac tgtcggtcag atacatcgac agcgccggcc ggctggcacg cgacaaccac
    15421 ctcgcctacc ggcgcatcaa aggcttgagc caaaccgtac tcaaccgcgt tcaggaagcg
    15481 gtcgtcgtca tcaacgtcga gcatcagacc atactgttca ataaaaaggc aaaagacctg
    15541 ctacccatgc ttgaaatcgg acagcatacc gccctgttcg accctattac cgttttatgg
    15601 gacaaggcct ctccgcacac tttcgaatac gtcctcggca catccggcct gaacgccggc
    15661 atccgcgccg ttccggtcaa caaagggccg gacaagctgc ttatcctcta catccgcccg
    15721 caaagcgaaa ttcaggcgga agccctgtcc gtcaaacttg ccgcgctcgg acaactgacc
    15781 gccaacctcg cccacgaaat ccgcaacccg atgtccgcca tccgccacgc caacgacctg
    15841 ctgcgcgaaa atatggaagc gggggcggca gatccgttca acgccaaatt gtgcaaaatc
    15901 atcgacggca acgtctgccg catcgacaaa atgctcgaag atatttcctc gctcaacaag
    15961 cgcaacaaaa ccgaacgcga aaccatcggc ctgataccgt tttgggaaga attcaaacaa
    16021 gagttcctgc tcggccatcc cgatgccgcc ggctgcatcc gcttggatat gcagggcaat
    16081 cacccgaccg cctatttcga tcccgcccac ctgcggcaga ttatgtggaa tctcgccaac
    16141 aacgcgtggc ggcacagccg caaacagccc ggctcgattt ccgtcaccat ccgccccgcg
    16201 caaaaaaaca ccgtctgcat cctctttgcc gaccgcccga agtgcaggaa cacctgttcg
    16261 aaccctttta caccacggcg gaaaacggca ccggcctcgg gctgtatgtc gcccgcgaac
    16321 tggcgcacgc caatttcggc gatttgacct acctaccgga agccaaatgt ttcgaactca
    16381 cattaccgga aaaaaccaat gactgaactg caacaccccg tcctcgtcgt cgatgacgaa
    16441 accgacattc tcgacctgat ggaaatgacc ctgatgaaaa tgggcttgcg cgtccatacc
    16501 gcgtcaggcg ttgccgaagc caaaaacaag ctcgacagcc aacgctattc gctcgtcctg
    16561 accgatatgc gtatgccgga cggctcgggg ctagaagtcg tccaacacat caacagccgc
    16621 ctgctcgata cgccggttgc cgtcatcacc gccttcggca atgccgatca ggcacaggaa
    16681 gccctgcgtt gcggcgcgtt cgaccccgat accatgcaga tacaggacta tctcgaccaa
    16741 atcgaacgcg acatcatcga acaaaccctc aaacaaaccg aaggcaaccg cacgcaggcc
    16801 gacaaacgct tgggcatcag cttccgttcc atgcgctacc gtatggaacg cctcaacatc
    16861 ggctgacgac aaaacggcat ccgcaccatc tccgcccacc cgaaaaaatg ccgtctgaaa
    16921 cggcacggga aagcgggttc gccccacgcc cgaacggaca caaaacacca tgaccgacat
    16981 ccttattgac aacaccgcca ccgaaaccgt ccgcaccctg atacgggcat tcccccttgt
    17041 gcccgtttcc caaccgcccg aacaaggcag ctacctcctt accgaacacg ataccgtcag
    17101 cctcaggctt gtcggggaaa aaagcagcgt catcgtcgat tttgcctccg gcgcggcaca
    17161 ataccggcgc acaaaaggcg ggggcgaact catcgccaaa gccgtcaacc acaccgcgca
    17221 ccccaccgtt tgggacgcaa ccgcaggatt ggggcgcgac agcttcgtcc tcgcctcgct
    17281 cgggctggcc gttaccgcct tcgagcaaca tcccgccgtc gcctgcctgc tttcagacgg
    17341 catccgccgc gccctcctca atcccgaaac gcaagacacc gccgcgcgca tcaccctgca
    17401 tttcggcaac gccgcagtac aaatgcccgc acttgttcaa acacaaggca aacccgacat
    17461 cgtctatctc gaccccatgt atcccgaacg ccgcaaaagt gccgccgtta aaaaagaaat
    17521 gacctacttc caccggctcg tcggcgaagc gcaagatgaa gcggcactcc tgcataccgc
    17581 acgccaaaca gcaaaaaaac gcgtcgtcgt caaacgcccc cgcctcggcg aacaccttgc
    17641 cggacaagcc cctgcctacc aatacaccgg caaaagcacc cgcttcgacg tttacctgcc
    17701 ctacgggacg gacaagggat aacgcccata aaacaagaca ccgaaaaatt tgccgttctt
    17761 atgcaaacga gaaaccggtt tttgcgtttc gactgttttg gataaatcat cacaccttaa
    17821 agtt
//
LOCUS       DAWGJV010000030        17310 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWGJV010000030 DAWGJV010000000
VERSION     DAWGJV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.030"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WHQ94_000709"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WHQ94_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ2128030.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WHQ94_000710"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WHQ94_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ2128031.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WHQ94_000711"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WHQ94_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ2128032.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3348..3497)
                     /locus_tag="WHQ94_000712"
     CDS             complement(3348..3497)
                     /locus_tag="WHQ94_000712"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2128033.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3494..3751)
                     /locus_tag="WHQ94_000713"
     CDS             complement(3494..3751)
                     /locus_tag="WHQ94_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128034.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMLINIG"
     gene            complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="WHQ94_000714"
     CDS             complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="WHQ94_000714"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ2128035.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="WHQ94_000715"
     CDS             complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="WHQ94_000715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ2128036.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTKEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12743..13114)
                     /gene="rplL"
                     /locus_tag="WHQ94_000716"
     CDS             complement(12743..13114)
                     /gene="rplL"
                     /locus_tag="WHQ94_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ2128037.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13172..13672)
                     /gene="rplJ"
                     /locus_tag="WHQ94_000717"
     CDS             complement(13172..13672)
                     /gene="rplJ"
                     /locus_tag="WHQ94_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ2128038.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13902..14597)
                     /gene="rplA"
                     /locus_tag="WHQ94_000718"
     CDS             complement(13902..14597)
                     /gene="rplA"
                     /locus_tag="WHQ94_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ2128039.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14597..15031)
                     /gene="rplK"
                     /locus_tag="WHQ94_000719"
     CDS             complement(14597..15031)
                     /gene="rplK"
                     /locus_tag="WHQ94_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ2128040.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15132..15668)
                     /gene="nusG"
                     /locus_tag="WHQ94_000720"
     CDS             complement(15132..15668)
                     /gene="nusG"
                     /locus_tag="WHQ94_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ2128041.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15670..15948)
                     /gene="secE"
                     /locus_tag="WHQ94_000721"
     CDS             complement(15670..15948)
                     /gene="secE"
                     /locus_tag="WHQ94_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ2128042.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16064..16139)
                     /locus_tag="WHQ94_000722"
     tRNA            complement(16064..16139)
                     /locus_tag="WHQ94_000722"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16104..16106),aa:Trp,seq:cca)
     gene            complement(16146..17279)
                     /gene="tuf"
                     /locus_tag="WHQ94_000723"
     CDS             complement(16146..17279)
                     /gene="tuf"
                     /locus_tag="WHQ94_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ2128043.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4325 a         4482 c         3891 g         4612 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcggac ggcctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgagcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggagtttc cacttcaact gccataatcg
      361 gctcaagcag ggcaggagat gcttgacgca taccttcttt aaacgcttga gaagcggcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gtacatcaac tacaggatag ccggcaacga taccgttagg caatgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacgccacc tttaatttca tcgataaact
      601 cgtaaccttc gccccccggt tccataggtt ccatttcaat cacaacgtga ccatattgac
      661 ctttaccacc ggattgtttc gcgtgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaaccg gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg gccttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg tttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtgacct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagtatacag tcaagcataa cgacaagaag aaagatgatt ttcttgtttg ccgataaata
     3301 tttaccgaca tccctcacaa tcagtcggac gactgaagaa aatactgtca atacgttttt
     3361 tctatactga caaaccgttc cttgccgctg cgcgaataaa aggcgggcat attgctgttg
     3421 atcttgttgt aacggaaatt tatttggggt gtaaatcctt tccacgacag ttttttatgc
     3481 cataaagccg cgctcatccg atgttaatca gcattcctat ccggtcaccc cgaacatcgc
     3541 cgcctgccgc gcttttagga acttcagacg gcattgcatc gcgaaccggc ggtacatcct
     3601 cggcaaacgg caggagggcg aggctttccc cttcagacgg catcgcaatc cgattaccga
     3661 cgttaatcag ttcgacaggt acgccgatat cctcggcaaa cgtcgccgaa gtcagcaggc
     3721 agcctgcaaa aaagagattt ctgggtttca tatttatttt ttaaaggaaa tgccgtctga
     3781 agtttcagac gacatttgtt taaatggaaa acattaagac gctacttttt ttgaccgccg
     3841 aaaccaacat ccctagcaaa gttattttgg tctccggacg taccgacgac acccgccacc
     3901 tcgttggtct ccggcccgaa aatacctaag gaataatcca cacgcctcaa aatgacgaaa
     3961 acgcttgttt ttgccttaat cagacaaaga aaaaccgcaa gcttaaagct tgcggttttg
     4021 tattgaataa acgcaccaaa gattattcat ccgttacttg ggtttcagca gtctcctgtt
     4081 ccacctcttg ccattgttga tgacggctgc ggtggtaagt caaaccggta ccggcaggaa
     4141 tcaggcggcc gacgatgacg ttctctttta aaccgcgcag ttcgtcttgt ttgcccataa
     4201 tcgcggcttc ggtcagaaca cgggtcgttt cttggaacga tgcggcagaa atgaaactgt
     4261 cggtggacag ggaggctttg gtaataccca gcaatacgtt ttcgtaacgt gccggctctt
     4321 tgccttcttc caaagctttt tcattggccg ccatcacatc gccgcgttcg acctgctctc
     4381 cggtaatgaa cccggtttcg ccggcatccg caatgttcac acggcgcaac atttgacgga
     4441 tgatgacttc gatgtgttta tcagaaatct tcacaccttg cagacggtaa acctcttgca
     4501 cctcttggac aatgtagcgt gccagtgctt cgataccttg caaacgcaga atatcatgcg
     4561 gatcaacagc tccgtccacg atggtttcac cgcggtttac cacttgaccg tcgtgtacca
     4621 gaatttgttt ctctttggaa atcaaggtct cgtatgctac accgtccacg tcagtaacaa
     4681 tcagacgttg cttgcctttg gtctctttgc cgaaggaaac ggtaccggta atttccgcca
     4741 acataccggc atctttcggc acgcgtgctt caaacaattc ggcaacgcgc ggcagaccac
     4801 cggtaatgtc gcgggttttg gaagaggctt gcgggatacg cgccaatacg tcgcctttac
     4861 cgatttcctg accttcgcgt acggtaatca ccgcaccaac ggggaatgcc atcgaaaccg
     4921 gagtggacgt accgggaata cagatttcca cgccgttttc atccaacaat tttacagtcg
     4981 gacgcagcag tttggacgcg ctgctggaac ggcgtttacc gtcaatcacc accaaagtgg
     5041 acaaaccggt tacatcgtca gtttgtttcg caacggtaac accctcttcc acgttctcaa
     5101 atttcaccat acctgcatgt tcggtgatca tcggacgggt atgcggatcc caggttgcca
     5161 aggtttgacc ggctttaatg gccataccgt cttgtaccag caggatggca ccgtaaggta
     5221 ctttgtggcg ttcgcgttca cggccgatat cgtcgtgaat cacgacttca caagaacggc
     5281 cgatgacaac caactcgcct ttattgttgg caacataacg catttggctg ctgaatcgtg
     5341 ccgtaccgtt ggatttggct tccacttggc tggctgctgc cgcacgggat gccgcaccac
     5401 cgatgtggaa cgtacgcata gtcagctggg tacccggttc gccgattgat tgggcggcaa
     5461 tcacaccaac tgcctcaccg gcattgacca gcttaccacg tgccaagtcg cgaccataac
     5521 agtgcgcgca caaaccgtgg cgggttttac aagtaatcgg cgtacggact ttgacttcat
     5581 cgacaccgga ttggtcaatc atatccacca gtttttcagt caacaacgta ccggcttcaa
     5641 ccaaggtctc gccgcttgac ggatcgacaa cgtcagacgc agtaacacgg cccaaaatac
     5701 gatcgcgcaa tgcttcaatc acatcaccgc cttgtaccac tgccttcata acaaagccgt
     5761 ctgaagtacc gcaatcgtct tcaacgacga ccaaatcttg agttacgtct accagacgac
     5821 gggtcaggta accggagttc gcggttttca atgcggtatc cgccaaaccc ttacgcgcac
     5881 cgtgggtcgc aataaagtat tgcaatacgg tcagaccttc gcggaagttt gaggtaatcg
     5941 gcgtttcaat aatcgagccg tcaggtttcg ccatcaaacc gcgcatacct gacaactgtt
     6001 taatctgagc cgcagaacca cgggcaccgg agtctgccat catatagatg gagttgaatg
     6061 actcttgatc gacttcgttg ccggcacggt cgataacttt ttgtttggac aggttgtcca
     6121 tcatcgcctt agcgatttta tcgccggcac gaccccaaat atcgaccacc ttgttgtagc
     6181 gttcgccgtt ggtaaccaaa ccttgacggt attggtcttc gatttcttta acctcggcat
     6241 tggcttcagc cagcaaggcc gctttttctt ttggaatttc catatcgtca acggcaatgg
     6301 aaataccgcc ttttgccgca aatccgaaac cggtgtacat caggtggtca gcaaagataa
     6361 ccgtatcgcg caagccgcac aggcggaacg atgcgttaat cagtttagaa atttcttttt
     6421 tcttcaacgc tttgttgaca tattcaaacg gcaggccttt cggcaggatt tcgctcaaca
     6481 atgcacggcc gacggttgtt tcgtaacggt taacgacagg ctcaaactca cctgcttcgt
     6541 ttttcaccca ttcgcgcaga cgtacggtga tttttgtacc cagctcgacc tgtttggtat
     6601 ggtatgcgcg atgcacttct ttcacatcgg caaacaggct gccttcgcct ttggcattga
     6661 tacggtcgcg ggtcatgtag tacagaccca atacgatatc ttgggaaggt acaataatcg
     6721 gttcgccgtt ggcaggagac aatacgttgt ttgaagccag catcagcgtg cgtgcttcca
     6781 tttgtgcttc caagctcaat ggaacgtgta ccgccatttg gtcgccgtca aagtcggcgt
     6841 tgaacgcggc acacaccaat gggtgcaact gaatcgcttt accttcaatc aagataggtt
     6901 caaacgcttg aatacccaaa cggtgcaggg tcggcgcacg gttcagcata atcggatgtt
     6961 cgcggatgac ttcttccaag atgtcccata cttccggtac ttcttgctct accaattttt
     7021 tcgctgcttt aacggtggaa gccagacctt gtttttccaa tttgtggaaa atgaacggtt
     7081 tgaacagttc caaagccatt tttttcggca gaccgcattg gtgcagacgc aggtatgggc
     7141 ctacggtaat cacggaacgg ccggagtagt ccacacgttt gcccaacagg ttttgacgga
     7201 agcgaccgcc tttaccttta atcatgtctg ccaatgattt cagcgggcgt ttgttggcgc
     7261 cggtcatggc tttaccgcga cggccgttat ccaacagcga gtcaactgct tcttgcaaca
     7321 tacgtttttc gttgcggacg atgatgtcgg gcgcatgcag ttccaacaga cgtttcagac
     7381 ggttgttacg gttaataacg cggcggtaca aatcgttcaa atcggaagtg gcaaaacgac
     7441 caccatccaa tggaaccaac ggacgcaaat caggcggcaa taccggcagc acatccataa
     7501 tcatccattc cagtttcata ccggaacgat ggaaggcttc caatactttc aagcgtttgg
     7561 cgattttttt gattttggtg tcggaaccgg tcgattccaa ctcttggcgc aggatttcga
     7621 tttcgcccgc tacattcagg gtacgcagca attcgcggat accttccgca cccattttgg
     7681 catcgaaatc gtcgccgtat tcgtccagct tgttgtagta atcgtcttca gtcagcaatt
     7741 ggcggcgttg cagcggagtc ataccgggat cggttaccac aaatgcttca aagtacaata
     7801 cgcgctcgat gtcgcgcaaa gtcatgtcta acaccatacc caagcgggaa ggcagggatt
     7861 ttaagaacca aatatgtgcg acgggcgcag ccaattcgat atgacccatg cgttcgcggc
     7921 gcactttgga cagggttact tccacgccgc atttttcaca cgttacgcct ttaaatttca
     7981 agcgtttgta ttttccgcac aagcattcgt agtctttgac cgggccaaag attttggcac
     8041 agaacaaacc gtcacgctca ggtttgaacg tacggtagtt gatggtttca ggttttttga
     8101 cttcgccata agaccatgag cggatggttt cgggagaggc aataccgatt ttaatggcat
     8161 caaactcttc ttccatgccg gcagtttgca acggattaaa taagttcaac aaattcattt
     8221 ttgctccttg aaggaagtat ttttaccgca tggcggattt tcgatattgg gaaacggtac
     8281 ttttgatttc agacggccta aatgccgatt gcttcattct tattctgaaa ccactttttc
     8341 agacgaccct gaaaggccgt ctgaaaactt ttgtttagta acgttccaaa tcgatatcca
     8401 agcccagtga gcgaatctct ttgaccaata cgttgaagga ctcgggcata ccggcatcga
     8461 ttttgtgttc gcctttgacg atgttttcgt acattttggt acggccgttc acgtcgtcag
     8521 acttcacagt cagcatctct tgcagcgtgt atgccgcgcc gtatgcttcc aatgcccaaa
     8581 cctccatctc gccgaaacgt tggccgccga actgggcttt accgcccaaa ggctgctggg
     8641 taaccagact gtacggaccg gtagaacgcg cgtgcatttt ttcgtcaacc aagtggtgca
     8701 gtttcagata gtgcatcaca cctactgtaa ccttgcggtc aaatgcttcg cctgaacggc
     8761 cgtcatacag cgtgatttgg gttttactgt cgttgaagcc cagtttttca acctcaggat
     8821 cgtcgctcgg ataagccaag ttcagcattt cgcggatttc agactctttc gcaccgtcga
     8881 atacaggaga ggcgaaagat gcacctttgc gcaggttgga ggccagttcg atgatttctt
     8941 catcagtcag ggcatccaaa tcttctttct taccgctgcc gttgtagagt ctgttcaaga
     9001 actcgcgcaa ctcgcctgct ttgcgttgct ctttcagcat acggtcgata cgctcgccga
     9061 taccttttgc tgcccaaccc aagtgaactt ccaaaatctg accgatgttc atacgggaag
     9121 gtacgcccaa tgggttcagt acgatgtcta ccggacggcc gtccgccatg taaggcatgt
     9181 cttccactgg cagaatgcgc gataccacgc ctttgttacc gtggcggccc gccattttgt
     9241 cgccggcttg cagacggcgt ttgatggcga taaaaacttt caccattttt tgtacgccgg
     9301 gttgcaattc gtcgccttgg gtcagttttt tcttcttgat ttcgtataac tcgtccgctt
     9361 cttcgcgttt ttgttgcagg ctcactttaa tcagttccaa ctgcttggcc aaatcttcat
     9421 cggtcagacg gatatcgaac caatcgtgcc tgctcggcag acccgccaaa tattcggtag
     9481 taatttcgct gcctttagcc agcttcatcg gaccaccatt ggctttttga cccacaatca
     9541 tacgttcgat acggtcgaat gcgtcgttgt cgaaaatacg caattggtcg ttcaaatcca
     9601 aacggtagcg tttcaactcg gaatcgataa tggattgggc gcgtttgtcg cgttgaatac
     9661 cttcacgagt gaagacttga acgtcgataa ccgtaccgct cataccggta ggcatacgca
     9721 atgaagtatc ttttacgtca gacgcttttt cgccgaagat ggcgcgcaac agtttttctt
     9781 ccggcgtcag ctgggtttcg cctttaggcg ttactttgcc taccaacaca tcgccggctt
     9841 ctacttctgc accgatgtaa acgataccgg attcgtccaa gcggttttgc atacgctcgg
     9901 acaagttcgg aatatcgcgg gtaatgtctt ccgcacccag cttggtatcg cgggcaacga
     9961 cattcaattc ctcaatgtga atcgaagtgt aacgatcatc cgcagccact ttttctgaaa
    10021 tcagaatcga gtcttcatag ttgtaaccgt tccatggcat gaaggcgatg gtcatgtttt
    10081 gacccaaagc caattcgccc aagtcggtag acgcaccgtc ggccaccaag tcgccgcgtt
    10141 gcaacacgtc gcctgctttt acggccggac gttggttgat gttggtagat tggttggaac
    10201 gggtaaattt aaccaagttg taaatatcga cacccacttc gccggcagtc gcttcgtcgt
    10261 catggacacg aatcacaaca cggttggcat cgacatactc gaccacgccg cctcggcggg
    10321 caacgattgc agtagcagag tcaacggcaa cggaacgctc gataccggta ccgaccatcg
    10381 gtttttcagg acgcaagcaa ggcactgcct gacgttgcat attcgcaccc atcaatgcgc
    10441 ggttcgcgtc atcgtgttcc aagaacggaa tcagggatgc tgcaacggat accacttgac
    10501 cagttgccac gtccatatat tggacgcggt cgggcgttgc cataatggtt tcgccttttt
    10561 cacgacaggt aaccaaatcg ccaatcagat tgccatctga atccaaatcg gcattcgcct
    10621 gtgcaatcac atagcggcct tcttcgatgg cagacaagta atcgatttcc ttggttactt
    10681 tgccgtcgat aacgcggcgg taaggcgttt ccaagaaacc gtaatcattg gtgcgcgcat
    10741 aaacggacaa tgagttgatc aaaccgatgt tcggaccttc aggcgtttca atcggacata
    10801 cgcggccgta gtgggtcgga tgcacgtccc gtacctcgaa gccggcacgt tcgcgggtca
    10861 aaccgcccgg acccaatgcg gatacgcggc gtttatgggt tacttcagac aaggggttgg
    10921 tctgatccat aaactgactc aattggctgg agccgaagaa ttctttaata gcggcagaaa
    10981 caggttttgc attaatcaaa tcgtgcggca tcaagttttc tgattccgcc tgattcaaac
    11041 gttcttttac agcacgttcc acacgggcca aaccgctgcg gaattggttt tcagtcagct
    11101 cgcctaccga acgtactcgg cggttgccca agtgatcgat atcgtcaact tcaccatggc
    11161 cgttacgcaa ctcgaccaaa gtcgcaatcg aggcgacaat atcttcgacg ctcaggacat
    11221 aaccgccttt ttcggcagca ccggcaaacg tttcgttcag caggcggccg taccaagagt
    11281 tttgttgggc ttcggacagt ttttgttcgt atgtgcgcgt attaaatttc atacggccta
    11341 cgcgggacaa atcgtagctg tcttcactga agaacaagcg gttaaacaat tgctcgaccg
    11401 cctcttcggt gggcggttcg cccggacgca tcatacggta aatcgcaaca cgcgccgcct
    11461 gccggccggc agtctcatcc gtacgcaagg tgttggagat ataagcaccc tgatccaact
    11521 cattgatgta aagggtcgta atttctttta cgccgttgat atcaaatttg gccaacaact
    11581 cttctgtaat ttcatcattg gcagaagcca atacctcgcc ggtttccgaa tcaatcagat
    11641 cggcagccaa tgctttaccc agcaggcttt ccggttctac atccaaacgg gtcaggcctg
    11701 cattggtaat atcacggata tttttcgcag taatgcgctt acctttggca accaatacat
    11761 tgccttcttt atccaagata tcgaccttgg cagtttcgcc tttcagacgg cctgcgacca
    11821 aatcggtttg aacaccgttt gaagacaaat agaacgtttc tttgtcgtag aaaatatcca
    11881 agatttgctc attgccgtag cccaaagcct tcaacaaaat cgttaccggc attttacggc
    11941 ggcggtcgat acggaaatac agcaaatctt tcgggtcaaa ttcaaaatcc aaccatgaac
    12001 cgcggtaggg aatgatacgg gcggagaata acaacttgcc ggaagagtgc gtcttacctt
    12061 tgtcatgctc gaagaatacg ccgggcgaac ggtgcaactg ggagacaatc acacgctctg
    12121 tgccgttaat cacaaaagaa ccgctcgggg tcatcaacgg aatttcgccc atatacactt
    12181 cgttttcacg aacttctttt accgtcggtt tagatgcttc cttatccaaa atcaccaaac
    12241 ggatacgcgc gcgcaagggg gctgcataag tgattccgcg caactgacat tcgggaatat
    12301 cgaacaaagg ctcgcccaat gtgtaatgca caaactccaa tcgcgcataa ccgttatggc
    12361 tcacaatcgg gaaaatagaa ttaaatgccg cctgcagacc gtcatcggta cgtttgtcaa
    12421 aagcattttc cagctgcaaa aacttcgcat aagaatcaat ttgggttgct agcaagaaag
    12481 gaacttccaa aacattttcc cgctttgcaa aactcttacg gatacgtttt ttctcggtaa
    12541 acgaatagtt catacacact ccgaaaagta attttcaata ataggccgtc tgaaacaacg
    12601 gtacgcatca atctgatatt tacatttatt tgcaatgact tgataaaaac tatgcaaata
    12661 aatgtaaaca atacttgatg tttattttaa gaagcaaaat aaggctggca gaaaactgcc
    12721 agccttcata agaagcatca aattatttga tttcgacttt agcgcctgct tcttccagtt
    12781 gtttttggat gtcttcggct tcagctttag aaacaccctc tttaatggtt ttaggtgcgc
    12841 cgtcaacgat gtctttagct tctttcagac ccaaaccggt aattgcacgg acaactttaa
    12901 tcacgccgac tttttgatca ccggcagaag ccaaaacgac atcaaattcg gttttttctt
    12961 cagcatcggc agcaccggca ccagcaggac ctgcaactgc aacagcagca gcagaaacac
    13021 cgaatttttc ttcaaaagct ttaaccaagt cgttcaattc cattacggtc aaagaaccaa
    13081 ctgcttccaa aatgtcttct ttagtaatag ccatgctatt ttactccaaa tattgtatta
    13141 aaaaataatt gattaacaga aacaaaatcg attaagcggc ttcttcgccg gcttttttct
    13201 ctgccaaagc agccaaaccg cgcgcaaagc ccgatacagg agcttgcata acgaacaaca
    13261 gtttggacaa cagctcttcg cggctcggaa tagaagccaa ctcagcaacc tgagcagcat
    13321 tcattacttc gccattgtaa gaaccggctt taacgacaat tttgtcatct tttttcgcga
    13381 attggtgcaa cactttagca gcagcaacag catcttcaga agcagcgtaa accaacggac
    13441 caaccatttg atcggccaat tctgcaaatg aagtaccttg cactgcacga cgagccaaag
    13501 tatttttcag aacacgcaaa taaacgcctt ctttacgtgc attcgcacga agctcagtca
    13561 tactggaaac actgataccg cgatattcag cgactacgag ggtttgagcg ttagcaattg
    13621 ccgcgctaat ttcctcgacc gccactttct tggtttcaat attgagactc aaggtctacc
    13681 tcccactgtt tataaacagg ataccaaaac gatatcccac cagcgcggta acctaaaaag
    13741 acatcttaca agcaatcttg caatgtgttt caggactacc gtctgcgtag ggcagttacg
    13801 attaaatctt acgatcccta cggtcttgga catctactta attttaagta gcccaaattc
    13861 aagcagccca gaaaaccaaa ctgcttgaaa attccttaag attagttatt tacgcttgat
    13921 gtatcaacgc gaatacccaa acccatggtg ctagacacag caactttttt cagatactga
    13981 cctttagcgg cagcaggctt ggctttaacg atagcatcca gcaacgcatc aaagttctct
    14041 ttcaaatcag cttcagcgaa tgaagcacga ccaatcgttg catgaacaat acctgccttg
    14101 tcggtacggt actgcacttg acctgctttt gcatttttta ctgcttcggc aacattaggg
    14161 gtaaccgtac ctactttagg gtttggcatc agaccacgag gacccaagat ggtacccaat
    14221 tgaccaacaa tacgcattgc atcgggagaa gcaataacaa cgtcaaagtt cagattgcct
    14281 gctttgattt cagcagccaa atcttcgaaa ccgacgatat ctgcaccagc ttctttagca
    14341 gcttctgcat ttgcaccttg agtaaataca gccacacggg ttatcttacc ggtgccttta
    14401 ggcagaacga ccgaaccacg gataacttgg tcagatttac gcggatcaac gcccaagttg
    14461 aaagatacgt caacagactc gtcaaattta gcagtcgctg ctttttttac caaagcaatt
    14521 gcttcatcaa ttgcatataa tttattggct tccacagaag agcgaagagc tttcaagcgt
    14581 ttagatactt tagccattat acaacaccct ccacatccaa gcccattgag cgagcagaac
    14641 ctgctatagt acggacagcc gcatccaagt cagcagcagt caaatcagga tctttagttt
    14701 tagcaatttc ttccaactgg gcacgggtca atttacccac tttgttggtc agaggattag
    14761 aactaccttt ttgcaaaccg gcagcctttt tcaacaagat agaagctggc ggggttttca
    14821 tcacaaatgt gaatgattta tctgcaaatg cagtaatcac aaccggaatc ggtaagccag
    14881 gctccatacc ttgggttgca gcattaaatg ccttacaaaa ttccataata ttcaaaccgc
    14941 gctgacccaa agcaggacca accggaggag atggattggc tttacctgca ggaatttgca
    15001 gtttaatata gccgataatt ttctttgcca cttaacgaac tcctaaaaac gggtaagacg
    15061 cggaaccatt tccgctcccc aagttaattt ttgattctat cctcttattg cttttttttc
    15121 aagtataaaa atcagttaat cttttcaacc tggctgaact ccagctcaac gggtgtttct
    15181 ctaccaaata tctgaacaga cacgcgtaac ttattccgtt cataattgac ctcctcaacc
    15241 accccgttaa aatccgcaaa cggcccttca tttacacgaa cctgttgacc gacctcaaat
    15301 tcaacttttg gtttcggctt ctctatgccg gtctgaacct gctgtaaaat aatttcagcc
    15361 tctctctgac taatcggcgt aggtctatta gccctccctc caataaaacc ggaaacacgg
    15421 ggggtgcttt ttacaagatg ccaagagtca tctgtcattt ccatctcaac tagcacataa
    15481 ccaggatatg actttctttc actaatagtc ttacgaccat tgcggatatc aacaactttc
    15541 tctacaggca ccagaatttg tccgaaataa tctcccatct cctcacgggc aatgcgctct
    15601 tccaatattc gttggacatt cttctcaaac cccgaatacg cctgtacaac ataccatttt
    15661 ttcgacatct caaccttccc ttctcagcaa tacatcaaaa aataaccacg aaattgctgt
    15721 atctgccgca tagataaata tagaaagcac agcaacaaac actataacaa atacagtcat
    15781 tctgacagca tcttcacgct taggccaaac cacctttttg aattcggacc aagaatttga
    15841 gaaatatgca aaaaaccctt ccttaccgga attagatgca gattctttat cttgaacaac
    15901 cagttgatcc actttaacgt tctttttttc aggcgtatgt tctgtcatat tatttatcca
    15961 tcatagcatc tgtctattct caatccatgt aaatggcaag agagtttact aaataacaaa
    16021 tacaaaaaaa attaaccggc acaaggccgg ttaatttttt atttggcagg ccaagagggt
    16081 ctcgaacccc caaccctcgg ttttggagac cgatactcta ccaattgagc tattggcctc
    16141 taaacttaag cgataacaga agaaaccacg ccggcaccca cggtacggcc gccttcgcga
    16201 atcgcaaagc gcaaaccttc ttccatagcg ataggcgcaa tcagttctac ggtgatggtt
    16261 acgttttcac ccggcattac catttctaca ccttcttcca aagtaaccgc gccagttacg
    16321 tcggtggtac ggaagtagaa ttgcggacgg tagttagcga agaacggggt atggcggcca
    16381 ccctcttctt tgctcagtac gtatacttct gctttgaact tggtgtgagg agtgattgta
    16441 cccggtttag ccaatacttg accacgctct acgtcttcac gtttggtacc gcgcagcaat
    16501 acgcctacgt tgtcgcctgc ttgaccttcg tccagcagtt tgcggaacat ttcaacaccg
    16561 gtacaagtgg ttttttgagt ttctttcaga cctacgattt cgatctcgtc accaacgtgg
    16621 atgataccgc gctctacacg accggttact actgtaccac gaccggaaat agagaatacg
    16681 tcttcgatag gcagcaggaa cggtttgtcc acggcacgct cgggagtcgg gatgtagctg
    16741 tccaatgcgg cagccagttc gaagattttc tcttcataag cggcatcgcc ttccaaggct
    16801 ttcagtgcag aaccttgtac aatcgggcag tcgtcgccgg ggaagtcgta gctggacagc
    16861 aggtcgcgga tttccatttc aaccagttcc aacagctcgg catcgtcgac catgtcgcat
    16921 ttgttcatga acacgatgat gtaaggtacg cctacttgac gggccagcag gatgtgttcg
    16981 cgggtttgcg gcatagggcc gtcggctgcg gaacatacca ggattgcacc gtccatttgt
    17041 gcggcgccgg taatcatgtt tttaacgtag tcggcgtgcc ccgggcagtc tacgtgtgcg
    17101 tagtggcggg tttcggtttc gtattccacg tgcgaggtgt taatggtaat accgcgtgct
    17161 ttttcttcgg gtgcgttgtc gatttggtcg taagcttttg cagcaccacc gaatttttta
    17221 gccaaaatag tagtcaaagc ggcagtcagg gtggttttac catggtcaac gtgaccgatg
    17281 gtgccaacgt ttacgtgcgg tttgctacgt
//
LOCUS       DAWGJV010000031        16742 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWGJV010000031 DAWGJV010000000
VERSION     DAWGJV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.031"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            263..907
                     /gene="rplC"
                     /locus_tag="WHQ94_000724"
     CDS             263..907
                     /gene="rplC"
                     /locus_tag="WHQ94_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ2128044.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            907..1527
                     /gene="rplD"
                     /locus_tag="WHQ94_000725"
     CDS             907..1527
                     /gene="rplD"
                     /locus_tag="WHQ94_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ2128045.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1524..1838
                     /gene="rplW"
                     /locus_tag="WHQ94_000726"
     CDS             1524..1838
                     /gene="rplW"
                     /locus_tag="WHQ94_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ2128046.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1844..2677
                     /gene="rplB"
                     /locus_tag="WHQ94_000727"
     CDS             1844..2677
                     /gene="rplB"
                     /locus_tag="WHQ94_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ2128047.1"
                     /translation="MAIVKMKPTSAGRRGMVRVATEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2684..2962
                     /gene="rpsS"
                     /locus_tag="WHQ94_000728"
     CDS             2684..2962
                     /gene="rpsS"
                     /locus_tag="WHQ94_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ2128048.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            2971..3300
                     /gene="rplV"
                     /locus_tag="WHQ94_000729"
     CDS             2971..3300
                     /gene="rplV"
                     /locus_tag="WHQ94_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ2128049.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3310..4002
                     /gene="rpsC"
                     /locus_tag="WHQ94_000730"
     CDS             3310..4002
                     /gene="rpsC"
                     /locus_tag="WHQ94_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ2128050.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            3986..4402
                     /gene="rplP"
                     /locus_tag="WHQ94_000731"
     CDS             3986..4402
                     /gene="rplP"
                     /locus_tag="WHQ94_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ2128051.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4402..4593
                     /gene="rpmC"
                     /locus_tag="WHQ94_000732"
     CDS             4402..4593
                     /gene="rpmC"
                     /locus_tag="WHQ94_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ2128052.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4593..4856
                     /gene="rpsQ"
                     /locus_tag="WHQ94_000733"
     CDS             4593..4856
                     /gene="rpsQ"
                     /locus_tag="WHQ94_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ2128053.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5079..5447
                     /gene="rplN"
                     /locus_tag="WHQ94_000734"
     CDS             5079..5447
                     /gene="rplN"
                     /locus_tag="WHQ94_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ2128054.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5459..5782
                     /gene="rplX"
                     /locus_tag="WHQ94_000735"
     CDS             5459..5782
                     /gene="rplX"
                     /locus_tag="WHQ94_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ2128055.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5792..6331
                     /gene="rplE"
                     /locus_tag="WHQ94_000736"
     CDS             5792..6331
                     /gene="rplE"
                     /locus_tag="WHQ94_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ2128056.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6334..6639
                     /gene="rpsN"
                     /locus_tag="WHQ94_000737"
     CDS             6334..6639
                     /gene="rpsN"
                     /locus_tag="WHQ94_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ2128057.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6655..7047
                     /gene="rpsH"
                     /locus_tag="WHQ94_000738"
     CDS             6655..7047
                     /gene="rpsH"
                     /locus_tag="WHQ94_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ2128058.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7061..7594
                     /gene="rplF"
                     /locus_tag="WHQ94_000739"
     CDS             7061..7594
                     /gene="rplF"
                     /locus_tag="WHQ94_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ2128059.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRTFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7608..7961
                     /gene="rplR"
                     /locus_tag="WHQ94_000740"
     CDS             7608..7961
                     /gene="rplR"
                     /locus_tag="WHQ94_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ2128060.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            7980..8498
                     /gene="rpsE"
                     /locus_tag="WHQ94_000741"
     CDS             7980..8498
                     /gene="rpsE"
                     /locus_tag="WHQ94_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ2128061.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8491..8676
                     /gene="rpmD"
                     /locus_tag="WHQ94_000742"
     CDS             8491..8676
                     /gene="rpmD"
                     /locus_tag="WHQ94_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ2128062.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8678..9112
                     /gene="rplO"
                     /locus_tag="WHQ94_000743"
     CDS             8678..9112
                     /gene="rplO"
                     /locus_tag="WHQ94_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ2128063.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9124..10434
                     /gene="secY"
                     /locus_tag="WHQ94_000744"
     CDS             9124..10434
                     /gene="secY"
                     /locus_tag="WHQ94_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ2128064.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10439..10657
                     /gene="infA"
                     /locus_tag="WHQ94_000745"
     CDS             10439..10657
                     /gene="infA"
                     /locus_tag="WHQ94_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ2128065.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10678..10791
                     /gene="rpmJ"
                     /locus_tag="WHQ94_000746"
     CDS             10678..10791
                     /gene="rpmJ"
                     /locus_tag="WHQ94_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ2128066.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10857..11219
                     /gene="rpsM"
                     /locus_tag="WHQ94_000747"
     CDS             10857..11219
                     /gene="rpsM"
                     /locus_tag="WHQ94_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ2128067.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11239..11634
                     /gene="rpsK"
                     /locus_tag="WHQ94_000748"
     CDS             11239..11634
                     /gene="rpsK"
                     /locus_tag="WHQ94_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ2128068.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11654..12274
                     /gene="rpsD"
                     /locus_tag="WHQ94_000749"
     CDS             11654..12274
                     /gene="rpsD"
                     /locus_tag="WHQ94_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ2128069.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12300..13286
                     /gene="rpoA"
                     /locus_tag="WHQ94_000750"
     CDS             12300..13286
                     /gene="rpoA"
                     /locus_tag="WHQ94_000750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ2128070.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13310..13678
                     /gene="rplQ"
                     /locus_tag="WHQ94_000751"
     CDS             13310..13678
                     /gene="rplQ"
                     /locus_tag="WHQ94_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ2128071.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13825..14538
                     /gene="minC"
                     /locus_tag="WHQ94_000752"
     CDS             13825..14538
                     /gene="minC"
                     /locus_tag="WHQ94_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ2128072.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14567..15382
                     /gene="minD"
                     /locus_tag="WHQ94_000753"
     CDS             14567..15382
                     /gene="minD"
                     /locus_tag="WHQ94_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ2128073.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15386..15649
                     /gene="minE"
                     /locus_tag="WHQ94_000754"
     CDS             15386..15649
                     /gene="minE"
                     /locus_tag="WHQ94_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ2128074.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15653..16573
                     /gene="oxyR"
                     /locus_tag="WHQ94_000755"
     CDS             15653..16573
                     /gene="oxyR"
                     /locus_tag="WHQ94_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ2128075.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16570..>16742)
                     /locus_tag="WHQ94_000756"
     CDS             complement(16570..>16742)
                     /locus_tag="WHQ94_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128076.1"
                     /translation="HCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNILAYRVVG
                     SGKWRFENNACA"
BASE COUNT         4592 a         3151 c         4161 g         4838 t
ORIGIN      
        1 atcaaccata ttccggtttg tcggagaaag atgcatacgc tgtgatgacc ggataccgac
       61 cctttaaaag agtccgaccc tatgccgtct gaaaattcaa aacgcttcag acggcatatt
      121 gaagatattt ctgatatttc tgttgatatt tctttaactt gtcagatata atgccgagct
      181 tggtacattt gtgccaagtt taactttgtc tgaaagacag gccaatcgca gcctgtccct
      241 ttactttaaa aggaaaataa tcatgacttt aggtctggtt ggacgcaaag ttggtatgac
      301 ccgcgtgttc gacgaacagg gtgtttctgt tccggtaacc gttttggata tgtcagccaa
      361 ccgcgtcaca caagtaaaat ccaaagatac tgacggctat actgccgttc aagttacctt
      421 tggtcagaaa aaagccaatc gtgtcaacaa agccgaagcc ggacactttg caaaagcagg
      481 tgttgaagcc ggtcgcggtt tgattgagtt tgctttgact gaagaaaaat tagctgaatt
      541 gaaagccggt gacgaaatca ccgtttctat gtttgaagtt ggtcaactgg tcgatgtaac
      601 cggtacctct aaaggtaaag gtttctccgg tacgatcaaa cgtcataact tcggcgccca
      661 gcgtacttcc cacggtaact cccgttctca ccgtgttcca ggctctatcg gtatggcgca
      721 agacccgggt cgcgtgttcc ccggtaaacg catggccggc caatacggca acaccaaagc
      781 aactgttcaa aaattggaag ttgtccgtgt tgacgcagaa cgccaactgc tgttggttaa
      841 gggtgctgtt ccaggtgcgg tcaacagcga tgttgtagtt cgtcccagcg tgaaagtagg
      901 tgcgtaatgg aattgaaagt aattgacgct aaaggacaag tttcaggcag tctgtctgtt
      961 tctgatgctt tgttcgcccg cgaatacaat gaagcgttgg ttcatcagct ggtaaatgcc
     1021 tacttggcaa acgcccgctc tggtaaccgt gctcaaaaga cccgtgccga agtaaaacac
     1081 tcaaccaaaa aaccatggcg tcaaaaaggt accggtcgcg cccgttccgg tatgacttct
     1141 tctccgctgt ggcgtaaagg cggtcgcgcg ttcccgaaca aacccgacga aaacttcact
     1201 caaaaagtaa accgcaaaat gtaccgtgcc ggtatggcga ctattctgtc ccaattggct
     1261 cgtgacgagc gtttgtttgc gattgaggcg ttgactgccg aaactcctaa aaccaaggtt
     1321 tttgctgaac aagtgaaaaa tctaggtctg gagcaagtgt tgtttgtaac caaacagctc
     1381 gacgagaatg tttacttggc ttcacgcaac ttgccaaacg tgttggtttt ggaagctcaa
     1441 caagttgatc cttatagctt gctgcgttac aaaaaagtaa tcatcactaa agatgcagtt
     1501 gcacaattag aggagcaatg ggtatgaatc aacaacgttt gactcaagtg attttggcac
     1561 ctatcgtttc tgaaaaaagc aacgtattgg ctgaaaaacg caaccaaatg acgtttaagg
     1621 ttttggcaaa tgcaaccaaa cctgaaatca aagctgctgt tgagctgctg tttggtgttc
     1681 aagttgcttc tgtaactacc gttacaacta aaggcaaaac taagcgtttt ggtcgtactt
     1741 tgggccgccg cagcgatgtt aaaaaagctt atgtaagctt ggctgccggt caagagttgg
     1801 atttggaagc cgctgctgca gctgcagata aggaataaac aaaatggcaa tcgttaaaat
     1861 gaagccgacc tctgcaggcc gtcgcggcat ggttcgcgtg gcaacagaag gtttgcacaa
     1921 aggtgcacct tatgcgccct tgcttgaaaa gaaaaattct actgccggcc gtaacaataa
     1981 tggtcatatc accacccgtc acaaaggcgg cggtcataaa caccattacc gtgttgtaga
     2041 ctttaaacgt aacaaagacg gcattcctgc caaagtagag cgtatcgaat acgatcctaa
     2101 ccgtactgcc ttcatcgcac tgttgtgcta tgcagacggc gagcgtcgtt acatcatcgc
     2161 tcctcgcggt attcaagtcg gtgccgtatt ggtttccggt gctgaagctg ccatcaaagt
     2221 aggcaacacc ctgccgatcc gcaacattcc cgttggtacg actatccact gtatcgaaat
     2281 gaaacccggt aaaggtgctc aaatcgcacg ttctgccggt gcttctgcgg tattgctggc
     2341 taaagaaggc gcgtacgctc aagtccgcct gcgctctggc gaagtccgta aaatcaacgt
     2401 agattgccgt gcaaccatcg gtgaagtcgg taacgaagag caaagcctga aaaaaatcgg
     2461 taaagccggt gccaatcgtt ggcgcggtat tcgtccgact gtacgtggtg ttgtcatgaa
     2521 ccctgtcgat cacccgcatg gtggtggtga aggccgtacg ggcgaggccc gcgaaccggt
     2581 cagcccatgg ggtactcctg ctaaaggcta ccgcactcgt aataacaaac gcacggataa
     2641 catgattgtt cgtcgccgtt actcaaataa aggttaattt agtatggctc gttcattgaa
     2701 aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgctc gcgcaagcaa
     2761 cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg attttatcgg
     2821 tctgaccatt gctgtgcaca acggccgcac ccatgtgcct gtgtttatca gcgacaatat
     2881 ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc acttggccga
     2941 taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca taaaaatgcc
     3001 cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa agacgttgcc
     3061 caagctttga atattttggc tttcagtcct aaaaaaggtg ccgagctgat taaaaaagta
     3121 ttggagtcag ctattgctaa tgccgagcac aataacggtg cggacattga tgaactgaaa
     3181 gtggtaacta tctttgttga caaaggccca agcttgaaac gttttcaagc tcgcgccaaa
     3241 ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt gggtaactaa
     3301 ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt aactaaagac
     3361 tgggcttcaa aatggtttgc taaaagcacc gacttttcta ctgttttgaa gcaggatatc
     3421 gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg agtggttatt
     3481 gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg tgtggttatc
     3541 ggtaaaaaag gtgaggatat cgaggttttg aaacgtgact tgcaagtctt gatgggtgta
     3601 cctgttcatg taaatattga agagattcgc cgtcctgagt tggatgctca aattattgct
     3661 gacggtattg cccagcagtt ggaaaagcgc gttcaattcc gtcgtgctat gaaacgagca
     3721 atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc aggccgtctg
     3781 aatggtgcgg atattgcccg tagtgaatgg tatcgtgaag gtcgcgtgcc actgcatact
     3841 ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg tgtattgggt
     3901 ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga acatgagagt
     3961 aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat accgtaagca
     4021 acaaaagggt cgcaatactg gcatcgctac tcgcggtaat aaggtaagtt tcggtgagtt
     4081 cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag ctgctcgtcg
     4141 tgcaatgacc cgtcatatca aacgtggtgg tcgtatttgg attcgtgtat tccctgataa
     4201 accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta acgtggaata
     4261 ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg ttccagagga
     4321 actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta caacctttgt
     4381 agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc cgttgagcag
     4441 ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat gcaaaacgct
     4501 accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat tgctcgtatt
     4561 aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat gttcgtactt
     4621 tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg gttgagcgta
     4681 aagtaaaaca tccgctgtat ggtaagatta ttcggttatc tactaaaatc catgcccatg
     4741 atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc cgtccattgt
     4801 caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct atttaagaat
     4861 taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt tgcgtgtaaa
     4921 cttcgtttcc tgtctttcag tttcttctgg aagtttcttc ccttttcggg gtccaagact
     4981 ggtttacttg aaccgcaagg tttcatttaa taagcagcgg ctttgctgta agttatctga
     5041 aagtggtaaa ttaagttggt taatttaaag gtaataacat gattcaaatg cagaccatct
     5101 tagatgtggc tgataactct ggtgcgcgtc gcgtaatgtg tatcaaggta ttgggcggat
     5161 ctaagcgtcg ctacgcttct gttggcgata ttattaaagt ggcagttaaa gatgcggctc
     5221 cgcgtggccg tgtcaaaaaa ggcgatgtat ataatgcggt agttgttcgt actgctaagg
     5281 gtgtacgtcg tcctgatggt gcgttaatta aattcgataa caatgccgcc gtgttactga
     5341 ataataaact tgaacctttg ggtactcgta tctttggtcc ggtaacccgt gaattgcgta
     5401 ctgagcgatt tatgaaaatc gtttcattgg cacctgaagt attataagga atggcacgat
     5461 gaataaaatc attaaaggcg atagggttgt agtaattgct ggtaaggata aaggtaagca
     5521 gggtcaagta gttcgagtgt tgggtgataa agttgttgtt gagggcgtta atgttgtaaa
     5581 acgccatcaa aaacctaatc caatgcgtgg cattgagggc ggtattatta ctaaagaaat
     5641 gcctttggat atttctaata tcgcaatcct gaatccggaa actaataaag cggaccgtgt
     5701 tggtattaag ctgattgaaa atgaaggcaa agttaaacgc gttcgtttct tcaaatcaaa
     5761 tggctctatc attggggcat aaggagataa catggctcgg ttgagagagt tttataaaga
     5821 gacagttgtt cctgaattgg ttaaacaatt tggttacaaa tcagtaatgg aagtcccgcg
     5881 tattgaaaaa attaccttga atatgggtgt gggtgaggct gttgctgata aaaaagttat
     5941 ggaacatgct gtttccgatt tagagaaaat tgccggtcaa aaaccggttg ttactgttgc
     6001 ccgtaaatct atcgcaggtt ttaaaatccg tgataactat ccggttggtt gcaaagtaac
     6061 attgcgtcgt gatcaaatgt ttgaattctt ggatcgtttg attactattg cattacctcg
     6121 cgtacgtgac ttccgtggtg tgagcggtaa atcatttgat ggccgtggca attacaatat
     6181 gggtgttcgt gagcaaatta tttttccgga aattgaatac gataaaattg atgctttgcg
     6241 tggtttgaat attactatta ctactacagc aaaaaccgat gaggaagcga aagctttatt
     6301 gtcattgttt aaatttccgt tcaaaggata atcatggcta agaaagcact tattaatcgt
     6361 gatctgaaac gtcaagcttt ggctaaaaaa tatgcggcta aacgcgcggc aattaaagcg
     6421 gtaatcaatg attcgaatgc aactgaggaa gagcgttttg aggctcgttt gaggtttcaa
     6481 tccattcctc gtaatgcggc acctgtgcgt caacgtcgtc gttgtgcttt gacaggtcgc
     6541 cctcgtggta ctttccgtaa atttggtttg ggtcgtatta aaatccgtga aatcgccatg
     6601 cgtggcgaaa ttccgggtgt tgttaaagcc agctggtaat aggagtaatt aagaatgagt
     6661 atgcatgatc ctatttccga tatgttgact cgtatccgca atgcgcaacg tgctaataaa
     6721 gcagcggttg caatgccttc ttcaaaatta aagtgtgcta ttgcaaaggt attgaaagaa
     6781 gaaggatata ttgaggactt cgcagtttca tctgacgtaa agtctatatt ggaaattcaa
     6841 ttaaaatact atgcaggtcg tcctgtaatt gaacaaatca agcgtgtatc tcgccctggt
     6901 ttgcgtattt ataaagcgtc tagtgagatt ccaagtgtta tgaatggctt gggtattgct
     6961 attgttagta cttctaaagg tgtaatgact gatcgtaaag cacgttctca aggtgttggt
     7021 ggtgagttgt tatgcattgt agcctagtgg aggaaaagaa atgtcacgtg tcgcaaaaaa
     7081 cccagtgact gttcccgctg gtgtagaagt aaaatttgga acagaggcat tagttattaa
     7141 gggtaagaac ggtgaattgt cttttccttt gcattctgat gtagccattg aatttaatga
     7201 tggcaaattg acttttgttg cgaataacag cagtaaacaa gcaaatgcaa tgtctggtac
     7261 tgctcgcgca ttagtcagca atatggttaa aggtgtttca gaaggttttg agaaaagatt
     7321 gcaattgata ggtgtgggtt atcgtgctca agcacaaggt aaaatcttga atctgtcttt
     7381 gggtttttct catccgatcg tatatgaaat gcctgaaggt gtctccgttc aaactcctag
     7441 ccaaacagag attgttttaa ccggctcgga taaacaagtt gttggtcaag ttgctgctga
     7501 gattcgtacg ttccgtgctc ctgagcctta taaaggtaaa ggtgttcgct atgtaggcga
     7561 agtagtggta atgaaagaag ccaagaaaaa ataattgagg ttcactaatg gataaacata
     7621 caacccgact ccgtcgtgca cgcaaaaccc gtgctcgtat tgcggacttg aaaatggtaa
     7681 gattatgtgt gttccgaagc aataatcata tttatgctca agtaattagt gctgaaggtg
     7741 ataaagtatt ggctcaagcc tctacattgg aagctgaggt gcgcggtagt ctgaaatctg
     7801 gaagcaatgt tgaagcagct gcaatagttg gtaaacgtat tgctgagaag gctaaagcag
     7861 caggtgtaga aaaggttgct tttgatcgtt caggtttcca atatcacggt cgtgtgaagg
     7921 ctttggctga agctgctcgt gaaaatggtt taagcttcta aatatttgga gactttcaga
     7981 tggcaaaaca tgaaattgaa gaacgcggtg acggtctgat tgaaaagatg gtcgcagtta
     8041 accgtgtaac caaagtagtt aaaggtggtc gcattatggc tttctctgcg ctaactgttg
     8101 ttggtgatgg agatggtcgt attggtatgg gtaaaggtaa gtcaaaagaa gtgcctgttg
     8161 cagtccaaaa ggcgatggat caagcacgac gctctatgat taaggtacca ttaaaaaatg
     8221 gtacgattca tcatgaggtt attggtcgac atggtgctac taaagtattt atgcagcctg
     8281 ctaaagaggg tagtggcgta aaagccggtg gacctatgcg tttggttttt gatgctatgg
     8341 gcattcataa tatctccgcc aaagtgcacg gatctactaa cccatataat atcgtacgtg
     8401 caacattaga tggtttgtct aagttgcata ctcctgctga tatcgcagcc aaacgtggct
     8461 tgacagtgga agacattttg ggagttaacc atggctgaac aaaaaaagat tagggttaca
     8521 ttggttaaaa gcctgattgg tacaattgaa tctcatcgtg catgtgcacg cggtttaggt
     8581 ttgtgtcgtc gcgagcatac ggtagaggtt ttagataccc ctgaaaaccg tggtatgatt
     8641 aataaaatca gctacttgtt gaaagtggag tcttgatatg tttttgaata caattcaacc
     8701 tgctgttggt gctacgcatg ctggtcgtcg tgttggacgc ggtattggta gtggtcttgg
     8761 caaaacgggt ggtcgtggtc ataaaggtca aaagagccgg tctggtgggt ttcataaggt
     8821 gggtttcgag ggtggtcaaa tgcccttgca acgacgcctc cctaaaagag gttttaaatc
     8881 tttaacagca tcagctaatg cacagcttcg tttaagtgaa ctggaatcaa ttgctgttaa
     8941 tgagattgat attttggtct taaagcaagc gggtctgatt gcatctacag tctctaatgt
     9001 taaagttatt gcttctggtg aaatttctaa ggcagttgct ttgaagggta ttaaagttac
     9061 caaaggtgcg agagctgcta tcgaggctgt tggtggtaag attgaaatgt aaggtttaat
     9121 attgtggcta atcaacaaac gtcatcaggt tcatccaaat ttggagatct taagaaacgt
     9181 cttttgtttc tatttggagc attgattgtt tttcgaattg gtgcccatat acccgtacct
     9241 ggagttgatg ctgttgcttt agctaaatta tacgaaagcg ctggaaacgg catcctggga
     9301 atattgaata tgttttccgg tgggtcgtta gagcgcttta gtatatttgc aataggaatt
     9361 atgccatata tttcagcttc tattattgta cagctcgctt ctgaaatttt gccatcattg
     9421 aaggctttaa aaaaagaagg ggaggctggt agaaaggtaa ttacgaaata tactaggtat
     9481 ggtactgttt tgttagcaat tcttcaaagt ctaggtgttg catctttcgt atttcagcaa
     9541 ggaattgttg taacaagttc atttgagttt catgtttcca cggtagtttc tttggtaacg
     9601 ggaaccatgt ttcttatgtg gcttggggag caaattactg aaaggggtat cgggaacggt
     9661 atttctttaa tcattacggc aggtattgct tcaggtattc cttcgggtat tgcaaagctg
     9721 gttacactga cgaaccaagg ttctatgagc atgcttacgg cgttgtttat tgtatttggt
     9781 gccttattat taatttattt ggttgtatac tttgaaagtg cacagcggaa gattcctatt
     9841 cattatgcaa aacgccagtt taatggtagg gcgggtagtc aaaatacgca tatgcctttc
     9901 aagttgaata tggctggtgt tattccccca atttttgctt ccagtattat tctatttcca
     9961 tctactcttt taggttggtt tggttcggct gatacaaata gtgttttgca caaaatagct
    10021 ggattgttac aacacggtca attgctgtat atggctttat ttgcagcgac agttattttc
    10081 ttttgttatt tttatacggc tttggttttt agccctaaag aaatggcaga gaatttaaaa
    10141 aagagtggtg cttttgttcc tgggattaga cctggtgagc agacctctag gtatttagaa
    10201 aaagttgtat tacgtttgac attgtttgga gctctttata ttacaactat ttgtttaatt
    10261 ccagagttct taactacggt tttaaatgta cctttttatt tgggtggcac gtctttgttg
    10321 attctagttg ttgtaacgat ggattttagt acacaaataa attcgtatag gcttactcaa
    10381 cagtatgata agttaatgac tcgttcagaa atgaaatcat tttctcggaa atagaattat
    10441 ggcgaaagaa gatactatcc aaatgcaagg tgaaattctt gaaactttac ctaatgcaac
    10501 atttaaagta aaacttgaga atgaccatat tgtattgggt catatttctg ggaagatgcg
    10561 gatgcattac attcgtattt ctccgggaga taaggtcaca gtagagctga caccttatga
    10621 tctaactagg gctcgaatcg ttttcagagc aagataaacc aataaaagga aaataaaatg
    10681 cgtgtacaac catctgttaa gaaaatttgc cgaaattgca agattattcg tcgaaatcgt
    10741 gtagttcgtg taatttgtac tgatctccgt cacaaacagc gtcaaggtta atggaatatt
    10801 tcttttaatg tgattctgtg atatagtgac acactttgcc ctaaaaagga aaaaatatgg
    10861 ctcgtattgc aggggtaaat atccctaata acgcacacat cgtaattggt cttcaggcta
    10921 tttacggtat tggtgctact cgtgctaaat tgatttgtga ggctgcaaat attgcgcctg
    10981 atactaaagc aaaagatttg gacgagactc aattagatgc tttgcgtgac caagttgcca
    11041 agtatgaagt agaaggtgat ttgcgtcgtg aggtaactat gagtatcaag cgattgatgg
    11101 acatgggctg ctatcgtggc ttccgtcatc gtcgcggctt accatgccgc ggtcaacgca
    11161 ctcgtacaaa tgcgcgtacc cgcaaaggtc cgcgtaaagc gattgctggt aagaaataaa
    11221 ttttaaggaa ttttattaat ggctaaagca aacacagctt cacgtgtacg taaaaaagta
    11281 cgtaaaaccg tgagtgaggg tattgtgcac gttcatgcat ctttcaacaa taccatcatt
    11341 acaatcactg accgtcaagg caatgcgttg tcttgggcta cctctggcgg cgctggtttt
    11401 aaaggttctc gtaaaagtac accatttgca gcacaagttg cagcagaagc agctggtaaa
    11461 gttgcccaag agtatggcgt taaaaattta gaggttcgta ttaaaggtcc aggtccaggt
    11521 cgtgaatcct ctgtacgtgc tttgaatgct cttggtttca agattaccag cattactgac
    11581 gttaccccgt tgcctcataa cggttgccgt ccgcctaaaa aacgtcgtat ttaatattgg
    11641 agtgatttga aacatggcac gttatattgg ccctaaatgt aagttggcac gtcgcgaagg
    11701 tacggatttg tttttgaaga gtgcgcgccg ctctttggat tctaaatgta aaattgattc
    11761 cgctcccggt cagcatggtg caaaaaaacc gcgtttgtca gactatggtt tgcagttgcg
    11821 tgaaaaacaa aaaatccgcc gtatttatgg cgtattagaa cgtcagttcc gtcgttattt
    11881 cgcagaagct gatcgtcgta aaggttctac cggcgagttg ctgttgcagt tgctggaatc
    11941 tcgtttggat aatgtcgttt atcgtatggg tttcggttct acccgagctg aagcaagaca
    12001 gcttgtttct cataaggcga tagttgtgaa tggacaagtt gtcaatattc cttctttcca
    12061 agtgaaagct ggtgatgttg tctcagttcg tgaaaaagcc aaaaaacagg tacgtattca
    12121 agaagcattg ggtttggcaa ctcaaatcgg cttgccgggt tgggtttctg tagatgcgga
    12181 taaacttgag ggtgtgttca aaaacatgcc ggatcgctcg gaattgaccg gtgatattaa
    12241 tgaacagctg gtggtagagt tctactctaa ataatgctag ctcagtgagg gacagttaaa
    12301 tgcagaatag cacaaccgaa tttttgaaac ctcgtcaaat tgatgtaaat actttttctg
    12361 caactcgtgc aaaagtatct atgcagccat ttgaacgtgg tttcggtcat accttaggta
    12421 atgctttgcg ccgtatctta ctgtcatcca tgaatggttt tgctcctact gaagtagcta
    12481 ttgccggtgt attacacgaa tattctactg ttgatggtat tcaggaagat gttgttgaca
    12541 ttttgctgaa tattaaaggt attgtgttta aactccatgg tcgtagccaa gttcaacttg
    12601 tgttgaagaa atcaggttca ggtgtcgtat ctgccggtga tattgagttg ccgcatgatg
    12661 tagaaattct gaatcctggt catgtcattt gtcatttggc tgataacggt caaattgaga
    12721 tggaaattaa agtagagcaa ggtcgtggtt atcaatctgt ttcaggtcgt caggtagttc
    12781 gtgatgagaa ccgtcagatt ggtgcaatcc agttggatgc gagcttttcg cccatcagcc
    12841 gtgttagctt tgaggttgaa cctgcacgtg tagagcagcg gacggatctt gataagttgg
    12901 ttttggatat cgaaaccgac ggttctattg atcctgagga agctgtacgc agtgcggcac
    12961 gtattttgat tgatcagatg tctatttttg ctgatttgca gggtactcct gtggaggagg
    13021 ttgaagaaaa agcacctcct atcgaccctg ttcttttgcg tccggtggat gatctggaat
    13081 tgacagtacg ttcagctaat tgtttgaaag ctgaggatat ttattatatt ggcgatttga
    13141 ttcaacgcac tgaaaccgag cttcttaaaa cgccgaattt gggacgtaaa tctttgaatg
    13201 agattaagga agtattggca tctaaaggtt tgacactggg ttctaagttg gaagcatggc
    13261 cacctgtagg cttggaaaag ccttaatgaa gaattaaagg ataattgata tgcgtcatcg
    13321 taatggcaat cgcaaattaa accgtaccag cagtcatcgt gctgcaatgc tgcgtaatat
    13381 ggcgaattca ttattgactc acgaaactat tgtaacaact ctgcctaagg ccaaggaatt
    13441 gcgccgtgta gtagagccgt tgattacatt gggtaaaaag ccgtcattgg caaaccgccg
    13501 tttggcattt gaccgtactc gcgaccgtga tgttgtagta aaactgtttg gcgatttggg
    13561 tcctcgtttt actgctcgta acggtggtta tgttcgggtg ttgaaatacg gattccgtaa
    13621 aggtgataat gcacctctgg cactggttga attggttgac aaaccggctg ctgagtaatt
    13681 ttagtcatat aacgccatct gccgaaaagc aggtggcgtt atttttgcaa tatctgatag
    13741 gtaatagggt attggctatc atgtttaaaa tattaattga atagctaagg tttgcgcggt
    13801 aaacttacat cattaaaaaa ttctatgatg gtttatataa tgaatgcttt cgatataaag
    13861 tcgacaaaga tggacgtatt gtctatatct ttgcatacgt cagacttgtt tgatttggaa
    13921 gatgtgctgg tcaaattggg caagaagttt caagagtctg gtgttgttcc atttgtgctg
    13981 gatgttcaag agtttgatta tcccgagtct ttggatcttg ctgcattggt ttcgttgttt
    14041 tcaaggcatg gtatgcaaat tttgggtctg aagcattcta atgaacgttg ggctgctgcg
    14101 gctatgaagt atcatttgct gttttgtctg tctcattcgg aaaatgttaa agaactgggt
    14161 caggttgagg tgcagaacac ggaggatggt cagaaagcaa ggaaaacagt attgattaca
    14221 tcccctgtcc gtaccggtca gcaggtttat gccgaagatg gcgatttgat tgttacgggg
    14281 gcggtcagcc agggggcgga attgattgca gatggcaata tgcatattta tgcgccgatg
    14341 agagggcgtg ctttggccgg tgccaaaggc gatacttctg cccgcatatt tatccactcc
    14401 atgcaggcag aactggtttc tgtggcgggt atttaccgta attttgaaca ggatttgccg
    14461 gaccatctgc acaagcagcc ggtacagata ttgttgcagg ataaccgatt ggttatcagt
    14521 gcaattggct cagaataatt gtttgatatt ttaaaaagga aatattgtgg caaaaattat
    14581 tgtagtaact tcaggtaagg gcggtgtcgg taaaacgact accagtgcca gtattgcgac
    14641 aggtttggca ttacgcggat ataaaactgc ggtaattgat tttgatgtgg gtttgcgtaa
    14701 cctcgacctc attatgggtt gcgagcgtcg tgtcgtttat gacctgatca atgtcattca
    14761 gggggaggcg acgctcaacc aggctttgat taaagataaa aattgtgaaa acctgtttat
    14821 tttgccggct tcccagactc gggataaaga cgctttgaca cgcgagggag tagaaaaagt
    14881 gatgcaggag ctgtccggca agaaaatggg ctttgagtat attatttgcg actctcctgc
    14941 cggtattgag cagggtgcat tgatggcgtt gtattttgct gatgaagcca ttgtaacgac
    15001 caatcctgag gtttccagtg tgcgtgactc cgacaggatt ttgggaattt tgcaaagcaa
    15061 atcccataag gcagagcaag gcggttcggt taaagaacat ctgttgatta cgcgttattc
    15121 tcccgaacgt gtggcaaaag gcgaaatgct gtctgtacag gatatttgcg atattctgca
    15181 tatccctttg ctgggtgtga ttcctgaatc ccaaaacgtc ttgcaggcat ccaattccgg
    15241 agaaccggtc atccatcagg acagcgtggc ggcttccgag gcatataagg acgttattgc
    15301 ccgtcttttg ggcgagaacc gtgaaatgcg cttcttggaa gctgagaaaa aaagcttctt
    15361 caaacgtctg tttggaggat aaggtatgtc attaatcgaa tttttattcg gcagaaagca
    15421 gaaaacggca accgttgccc gcgaccgcct tcaaatcatc attgcccaag agcgcgccca
    15481 agaaggtcag gctccggatt acctgccgac tttacgtaaa gagttgatgg aagtcctgtc
    15541 caaatatgtg aacgtttcat tagacaatat ccgtatttcc caggaaaaac aggatggtat
    15601 ggatgtgctt gagttgaaca ttactttgcc ggaacagaaa aaggtatagg acatgacctt
    15661 aaccgaattg cggtacatcg tcgcagtcgc ccaagaacgt catttcggca gggcggcgcg
    15721 gcgttgtttt gtcagtcagc ccactttgtc tattgccatt aagaaattgg aagaagagct
    15781 tgccgtctct ttgtttgacc ggagcagcaa cgatattatt acgaccgagg cgggggaacg
    15841 tatcgttgca caggcgcgta aggtattgga agaggcggag cttatcaggc atttggcaaa
    15901 tgaagaacaa aacgagctgg agggtgcgtt caaactcggg ctgattttta cggttgcgcc
    15961 gtacctgctg ccgaaactga ttgtctcgtt gcgccgtact gcaccgaaaa tgcctttgat
    16021 gttggaagag aattacacgc atactttgac cgagtcgctc aaacgcgggg acgttgatgc
    16081 gattatcgtt gccgaaccgt ttcaagagcc gggcattgtt accgaaccct tgtatgacga
    16141 accgtttttc gtgattgtcc cgaaagggca ttcatttgag gaactggatg ccgtttcgcc
    16201 ccggatgctg ggtgaggagc aggttttgct gctgacggaa ggcaactgta tgcgggatca
    16261 ggtactctca agctgttccg aattggcggc gaaacaacgc atacaggggt tgaccaatac
    16321 attgcagggc agctcgatca atacaatccg ccatatggtc gccagcggtt tggcaatcag
    16381 cgtgttaccg gcaaccgcgc tgaccgaaaa cgatcatatg ctgttcagca ttattccgtt
    16441 tgaaggtacg ccgccaagcc ggcgggtcgt attggcgtac cgccgcaatt ttgtccgtcc
    16501 gaaggcgttg tcggcgatga aggcggcgat tatgcagtcg cagcttcacg gggtaagttt
    16561 tatccgcgac taggcgcagg cattgttttc aaaacgccat ttccctgagc cgacaacacg
    16621 gtatgccaag atattgccgt catcatcgat tttgagtata gcatcgccac ggaaactgcc
    16681 gtcctgaaga tattcgactt ttgcatcact gtgaatgttt tcatcagtgc cgatgcaatg
    16741 cc
//
LOCUS       DAWGJV010000032        16428 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWGJV010000032 DAWGJV010000000
VERSION     DAWGJV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.032"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            117..1292
                     /locus_tag="WHQ94_000757"
     CDS             117..1292
                     /locus_tag="WHQ94_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ2128077.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            1340..3250
                     /gene="pglD"
                     /locus_tag="WHQ94_000758"
     CDS             1340..3250
                     /gene="pglD"
                     /locus_tag="WHQ94_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ2128078.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDPANWQSFLLTAFLTACLFFRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMAPVIIYGAGRSGRQLLKA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RLRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            3306..4598
                     /locus_tag="WHQ94_000759"
     CDS             3306..4598
                     /locus_tag="WHQ94_000759"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ2128079.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDNLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4650..5486)
                     /locus_tag="WHQ94_000760"
     CDS             complement(4650..5486)
                     /locus_tag="WHQ94_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ2128080.1"
                     /translation="MIDLHCHSTVSDGMLSPAEIVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIADKLEKKGIGGAYDGALELAANKEMVSRTHVAEFLIKNGYVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            5495..5737
                     /locus_tag="WHQ94_000761"
     CDS             5495..5737
                     /locus_tag="WHQ94_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128081.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            complement(5779..5988)
                     /locus_tag="WHQ94_000762"
     CDS             complement(5779..5988)
                     /locus_tag="WHQ94_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128082.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(5966..6199)
                     /locus_tag="WHQ94_000763"
     CDS             complement(5966..6199)
                     /locus_tag="WHQ94_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ2128083.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            6738..10172
                     /gene="dnaE"
                     /locus_tag="WHQ94_000764"
     CDS             6738..10172
                     /gene="dnaE"
                     /locus_tag="WHQ94_000764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ2128084.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQKRLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQDNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQMARERYARSLSLALAPHHDIGKLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10320..10469
                     /locus_tag="WHQ94_000765"
     CDS             10320..10469
                     /locus_tag="WHQ94_000765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128085.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(10606..11538)
                     /locus_tag="WHQ94_000766"
     CDS             complement(10606..11538)
                     /locus_tag="WHQ94_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2128086.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFSAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(11608..13764)
                     /locus_tag="WHQ94_000767"
     CDS             complement(11608..13764)
                     /locus_tag="WHQ94_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ2128087.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     PAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIESGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            13924..14583
                     /locus_tag="WHQ94_000768"
     CDS             13924..14583
                     /locus_tag="WHQ94_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ2128088.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(14670..15638)
                     /gene="ispH"
                     /locus_tag="WHQ94_000769"
                     /gene_synonym="lytB"
     CDS             complement(14670..15638)
                     /gene="ispH"
                     /locus_tag="WHQ94_000769"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ2128089.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(15668..16162)
                     /locus_tag="WHQ94_000770"
     CDS             complement(15668..16162)
                     /locus_tag="WHQ94_000770"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ2128090.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
BASE COUNT         3777 a         4822 c         4295 g         3534 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaacc cgacggacca aacaccatgc
      121 tgaacacctc cctctccccg tggccctgct ttacccaaga agaagccgat gccgtttcca
      181 aagtcctgct gtccaacaaa gtcaactact ggacgggcag cgaatgccgc aaatttgaaa
      241 aagaatttgc cgcctttgcc ggcacgcggt acgccgtcgc cctttccaac ggcacgctgg
      301 cactcgatgt cgcgctcaaa gcaatcggca taggcgcggg caacgatgtg attgttacct
      361 cgcgcacttt cttggcttcc gcttcctgca ttgtgaacgc gggcgcaaat cccgtgtttg
      421 ccgatgtgga tttgaacagc caaaacatca gcgccgaaac tatcaaagcc gcgctgacgt
      481 cgaataccaa agccatcatc gtcgtccacc tcgccggtat gcccgccgaa atggacggca
      541 tcatggactt ggcaaaagaa catgatttgt gggtcatcga agactgcgcc caagcgcacg
      601 gcgcgaaata caaaggcaaa tccgtcggca gcatcggaca cgtcggcgca tggtcgttct
      661 gccaagacaa aatcatgacc acgggcggcg aaggcggcat ggttacgacc aacgacaaaa
      721 ccctgtggga aaaaatgtgg tcgtacaaag accacggcaa aagctacgat gccgtgtacc
      781 accgcgaaca cgcgcccggt ttccgctggc tgcacgaaag tttcggcaca aactggcgta
      841 tgatggaaat gcaggcggca atcgggcgca tccagctcaa acgcctgccc gaatggactg
      901 cctgccgtca agcgcacgcc gccaagctgg cggaaagcct aggcaaattc aaaagtatcc
      961 gcttggttga agtcgccgac tacatcggac acgcgcaata taagttctac gctttcgtca
     1021 aacccgaaca cctcaaagac ggctggacgc gcgaccgcat cgtcagcgaa ctgaacgcgc
     1081 gcaaagtccc ctgctatcaa ggcagctgct ccgaagtcta tttggaaaaa tctttcgacg
     1141 acacgccttg gcggccgaaa gagcgtttga aaaatgctgt cgaactgggc gacaccagcc
     1201 tgatgttctt ggtgcacccg acgctgaccg acgacgaaat cgcgttctgc aaagaacaca
     1261 tcgaagccgt attggcagaa gccacacgat aaccattcag acggcatatg ccgtctgaaa
     1321 aatacacgcc gccgacaaca tgactctgga aaccctgatc gccctgccgc gcaacatcaa
     1381 gaaaatctgt ttcctcatac acgattttct gatgattttc attgcctttt ggttcaccca
     1441 aagcctaaag gccgactact cggacgaatg gttcgacccc gccaactggc agtctttcct
     1501 attgaccgcg tttttgaccg cctgcctgtt cttcagaatg gggctttacc gcgccgtcac
     1561 acgcttcatc agcttccgca tcctctccac cgcgctggca ggcagcctcg cctccgccgt
     1621 attgtttttc ctcaatacgc tgatatttga agaacggctg cggctcgccc tgccgattgt
     1681 ctatttctta ctgctgtttg tttccgtgac cggctcgcgt atggttttgc gcggactgtt
     1741 gtccgaacac cccaaaaaac agatggcccc cgtcattatt tacggcgcgg gacggtcggg
     1801 cagacaactg cttaaggccg tcaaacaaat gcgcgaatat tccgccgccg cctttgtgga
     1861 cgacgacccc aaactgtggc acaccgtcat ctacgacctt gccgtttacc agcccgatgc
     1921 catcgccttc ctcatcgaac gctacggcgt ggaaaaaatc ctgctcgcca tccccggcgc
     1981 gactcaggaa caacgcctcc gaatcatcaa caaactggaa gcctatccct gcgaagtgtt
     2041 gaccattccc ggaatgaaag acctgatgga cggaaaaatc agcatcggca cgctcaaaaa
     2101 aatctctgtg tccgacctgc tcgggcgtga ttccgtcgca ccagacgacc gcctcatgaa
     2161 tgccgacatc gaaggcaaaa ccgtcatggt aaccggcgcg ggcggctcca tcggttcgga
     2221 actctgccgc cagattatcc gccgccgccc cgaaaagctg ctgctgttcg agttatccga
     2281 attcgccctg tacgccatcg aaaaagaatt gcgcgaaacc tgcatccaaa aacgcctcga
     2341 taccgaaatc ctgccctttc tcggttcggt gcaaaaccgc acgctgctcg aacacgtcat
     2401 gaccgccttt tccgttgcga ccgtctatca cgccgccgcc tacaaacacg tccccatggt
     2461 cgagttcaac accgtcgaag gcatacgcaa caacatcttc ggcacactcg agtgcgcgct
     2521 tgccgccacg gcatcgggcg taagaacttt cgtcctcatc tccaccgaca aagccgtccg
     2581 ccccaccaac accatgggtg ccggcaaacg catggcggaa ctctgccttc aagcgctcgc
     2641 cgccgaaccc ggacaaaaaa cccgcttcag catggtgcgt ttcggcaatg ttttaggttc
     2701 gtccggctcc gttgtcccgc tgtttgaaaa acagattgcc gaaggcggcc cgcttaccct
     2761 gacccaccct gaaatcacac gatatttcat gaccataccc gaagccgccc aactcgtcat
     2821 acaggcaggt gcgatgggta cgggcggcga cgtattcgtc ctcgacatgg gcgaatccgt
     2881 caaaatcatc gaccttgccc gccaaatgat taccttaagc ggcctcaaac ccaaaacacc
     2941 cgaacaaccc gacggcgaca tcgaaatcct cattaccgga ctgcgtcccg gagaaaaact
     3001 ctacgaagag ctgctcatcg gcgacaacgt ccgcaaaacc ggccatccgc gcattatgac
     3061 ggctgacgag gcgatgctgc cgtggcacga gctctctgtc ctgctcgacc gcatccgtgc
     3121 ggcctgcgac cgttacgacc agcaggcaat ccgcaccctg ctcataaacg ccccgaccgg
     3181 ctttacccca agcgacggta tctgcgacct gctttgggta cgagaaacac acagaaaaaa
     3241 tgccgtctga accttcagac ggcataacgt acaaaccaac ctaccttaca cacacggagt
     3301 ttgacatgca gttttcagca ttcggcgaaa aattcacgca acacagcggc atcctccaac
     3361 taatggacaa cctcggcgac gcgctcaaaa gcgacaagcc cgtcaacatg ctcggcggcg
     3421 gcaatccggc gcgcattccg gaaatcgatc gggcgtttgc cgacatattt tccaaactgg
     3481 cggcagaaca cgccgtcgaa aacatcggca actactccaa tccccaaggc gatgccgtgc
     3541 tgattgacgc gctgaccgcc ttcctcaacc gcgaatacag ctggaatctg accgccgaca
     3601 atatcgcgct gaccaacggt tcgcaaaacg cgtttttcta tcttttcaac ctcttcggcg
     3661 gcaaattcaa gctttcagac ggcacatccg cagaaaaagc cattttgttg ccgctcgcgc
     3721 ccgaatacat cggctatgcc gacgtgcatg tcgaagggca gcacttcgtt tccgtcaagc
     3781 ccaaaatcga aaacgtcgaa cacgaaggcg aagcaggctt cttcaaatac cgcgtggact
     3841 ttgacgcact ggaaaacctg cccgaactca aagcgggcaa aatcggcgcg atttgctgtt
     3901 cgcgcccgac caacccgacc ggcaatgtgt tgaccgacgg cgaaatggcg cgtttggacg
     3961 ctttggcgcg tgaacacggg attccgctga ttatcgacaa cgcctacgga atgccgttcc
     4021 ccaacatcat ttacagcgac gtaacgctga attggcatga aaacatcatc ctctgcttca
     4081 gcctttccaa agtcggcctg ccgggcgttc gcaccggcat catcgtcgcc gcgccggaag
     4141 tggtcaaagc cgtcagcagc ctgaacgcga ttgtgaacct tgcccccacg cgcttcggcg
     4201 cggccatcgc cgccccgcta ctggaaagcg gcgagatgaa acggcttgcc gaccaagtca
     4261 tccggccgtt ttaccgcaat caggcgcaaa ccgccgtctc gctgctcaag cgcgagctgg
     4321 gcgcgtaccc gatgaaaatc cacaaacccg aaggcgcgat tttcctgtgg ctctggtttg
     4381 aaaacctgcc cgtttcttcg caaaccctgt acgaaatgct caaagccgaa ggtacgctga
     4441 ttattccggg cgaacatttc ttcgtcggca tcgacacgca ggattacccg cacgcgggcg
     4501 agtgcatccg catgagcatc gcgcaggacg ctcaaacgct ggaaaaaggc attgccgcca
     4561 tcggcaaaac cgtccgaaaa ctgtacgaca acgtttaaac cgcaaaaaat gccgtctgaa
     4621 aggttttcag acggcatttt tatctgcatt caatgtcggg aaaaatgcgc ccacaccggc
     4681 ctgcagtttt ccggcagctc gggacacgcg ccgaggatgc cgccgctgaa gtcgtttaaa
     4741 cggtggaagt cgctgcccgc gctggcgagc ataccgaagc gttctgccaa aagcgcgtag
     4801 ttgaggcggt cgtttttgca acagtttccg ctgtggactt cgatgcctgc gccgccgagg
     4861 tttttaaatt cttcaaacag attgcgcttg gcggtggcgg acaaatcgta gcgcatcgga
     4921 tgggcaatca ccgccaaccc acccgcgccg ttgacggcgg agacgcagtc ttccagcgtc
     4981 gcccattcgt gtcggacggc gcaggatttg ccgtcgccca agtatttggt gaacgcctgc
     5041 tgcttgtttt tgacgtatcc gtttttgatg aggaactcgg cgacgtgggt gcggctgacc
     5101 atttctttgt ttgccgccaa ctccagcgcg ccgtcgtatg cgccgccgat gcctttcttt
     5161 tcgagcttgt cggcgatggc ttcaagacgt ttcagacggc cttgccgcac ttgcgccaac
     5221 aagttttgca ggttctcgtc ctgctcgtcg aaatccaaac cgacaacgtg tatggtacgc
     5281 ccgcgccacg ttacggagat ttccacgccg ttaatcaggc gcaaaccgag cctgtcggct
     5341 tcggcacgcg cttcggcgat gccgcccgtg tggtcgtggt cggtcaacgc cagcagcgtg
     5401 cagccatttt gatgcgcgag gcgcacgatt tcggcggggg agagcatacc gtcggaaacg
     5461 gtggaatggc agtgcaggtc tatcataagg cgttatgtgc gtggtaaatg gtgggcaggg
     5521 tgtttcaata ttgtcgggcg gtgtggcggc ggtgatttca aaaaacaagt gtatggcaaa
     5581 tgcaaaggaa aagccgctat gccgtcattc ccgcgcaggc gggaatccag actttgattt
     5641 attggaaatg tttaaggtta accgctattt caaacttccg gattcctgct ttcgagggaa
     5701 tggcgatatg accgttttca attttaatct actataaaag actgtctgaa aacgtggttt
     5761 tatcaagccg aactggtttc agacggcatt ttcgtcttcc gcccaacccg cttctttcaa
     5821 cttccgccgt tgcgcgtcgt agcgtgcctt atccgcccag taaatcgtct gaatgcacgc
     5881 ctcgccgcag tcgctgccgc agcattccca cgactcgggt cggacgggtt cgtctaaaag
     5941 cggctcgccc aaaagggctt cggctttatg cttcagggtc atatccatcg gcgatttcca
     6001 agcgcgcacc gtcaaactcg atgacttcgc cgccgcgtat tttggcggtt ttacgggttt
     6061 cggtttcgcc gttgcgcgac accaaccctt cggcaatcaa cgccttcgcc tgtccgccgc
     6121 tttcggcaag tccggccaat ttcaagaggt cgcacagggc gatgtattcg ttgtcttcga
     6181 gatagacggt ggcttccata atgttccctt gcagaaagag gccgttattg tagcacctgc
     6241 cgccgccgta cccaaaatta ccgcaaaacc ggcagcacat cccacaccag ctgttccgta
     6301 aaagtaaaaa tgccgtctga aaccccatat gccgcaatcc gttcaaagaa atcctgccca
     6361 acggcagact gcaaatcctg ttccccgacg aatccgcatt gacgctgatg cacatcctca
     6421 aacgcgaact gcccgataca ccggcaatcg gcatcaaaac gaaatcaaaa acctgtttga
     6481 acgtaaaatt aatttaactg cttgccatcc gtccgaataa ggtatatagt tctttataac
     6541 tagttttata gtcctttata tctatcaata ctccttggga agcctccacc acacggtagg
     6601 aggcattttt ttgccgtcgt aaaagctcaa aaacatttgc aggtcatgcc gtctgaatct
     6661 gaaacggcat gacctacacc gccatctaaa gacaaccctg ctacaatacg ccttttattg
     6721 ttcacgccga ttttgccatg accgagccga cctacatccc cctgcgcctg cataccgagt
     6781 tctcaattac cgacggtatg gtgcggatta aaaaactgat tgccaaagcg caggaatacg
     6841 gtttgcctgc tttgggcatc agcgatttga tgaacgaatt cggtttggtg aaattctata
     6901 aagcctgccg cggcgcaggg attaagccca tcggcgcggc ggacgtgtgg ataggcaatc
     6961 cgaatgcgcc cgacaagccg ttccgcgcta tgctgattat ccgcaacgat gcgggctatc
     7021 tgcgtttgag cgagcttttg acagaggcct atgtcggcaa agaccgcaat atccatcatg
     7081 cggaactcaa ccccgaatgg ctggaaaacg gcgacaacag cggcttgatt tgtttgagcg
     7141 gcgcgcatta cggcgaagtg ggcatgaatc tgttgaacgg caatgaagac gcggcgcgcg
     7201 cggcggcgtt gaaatatgcg gcatggtttc ccgatgcgtt ttatttagag ctgcaacgcc
     7261 tgcccgaacg ccccgaatgg gaggcttgcg tttccggcag cgtgaagctg gcggaggaat
     7321 tgggtttgcc ggtggtggcg acgcatccga cacagtttat gagccgcgac gatttcaacg
     7381 cgcacgaggc gcgagtgtgt atcgcaggcg gctgggtatt gacggacaag aaacgtccgc
     7441 gcgatttcac gccgggccag tttttcattc cgccggaaac catggccgaa cgtttcgccg
     7501 atttgcctga agccttggaa aacacggtag aaattgccaa acgctgcaac ctgcacatca
     7561 cattgggcaa aaacttcctg ccccttttcc ccacgcccga cggtttatca ctcgatgact
     7621 atctcatcaa actctccaac gagggtttgc aggaacgtat ggttcagctt tatcccgacg
     7681 aggcggagcg ggcggcgaaa atgccggaat atcaaaaacg gctggatttt gagctgaaca
     7741 tcattatcca gatgaaattc cccggctatt tccttatcgt acaagacttt atcaactggg
     7801 cgaaaacaca cggctgtccg gtcgggccgg gccgtggttc gggcgcgggt tcgctggtgg
     7861 cgtattcatt gaagattacc gaccttgatc cgctcaaata cgcgctgctg ttcgagcgtt
     7921 tcctaaaccc cgaacgcgtt tctatgcccg acttcgacgt ggacttttgc caaagcaacc
     7981 gcggccgcgt gattgaatat gtgcgcgaga aatacggcgc ggaggcggtc agccagattg
     8041 ttaccttcgg cacgatgtcg tccaaagcgg tcatccgcga cgtcgggcgc gtgttagagc
     8101 tgccgtttat gctgtgcgac aaactgtcca agctgattcc gttggaagcc aacaaacccc
     8161 tgagtttgga aaaagccatg gagaccgagc cgcagatcca ggaattgatc gaagcggaag
     8221 aagcggacga actgattacg ctggcgaaaa agctggaaga tttaacgcgc ggtttgggta
     8281 tgcacgcagg cggcgtgttg attgcgccgg gcaagatttc cgattacagc cccgtgtatc
     8341 aggcggacga atccgcctcg cccgtatcca tgtacgacaa gggcgacgtg gaagatgtgg
     8401 gtttggtgaa attcgacttt ttgggtctgc gcaacctgac cattatcgaa atggcgcagg
     8461 acaacatcaa aaacactacc ggcgacatca tcgatgtcgg caaaatcccg cttgacgacc
     8521 aggtcgccta ccaaatcttc cgcgatgcga acaccaccgc cgtcttccag ttcgagtcga
     8581 ccggcatgaa aaaaatgctg aaaacggcgc acacgaccaa gtttgaagaa ctcatcgcct
     8641 tcgtatcgct ctaccgcccc ggcccgatgg acaacattcc cgacttcgtc gcacgtatga
     8701 aggggcaaga attccaatac atccatccgc tactcgaagg catcctcgcg ccgacctacg
     8761 ggattatggt gtatcaggaa caagtgatgc aggcggcgca aattatcggc ggttactcgc
     8821 tcggcggcgc ggacttgttg cgccgcgcca tgggtaagaa aaagcccgaa gaaatggtga
     8881 aacaccgcga aatcttcgcc gaaggcgcgg caaaacaagg catttcgcgc gaaaaatccg
     8941 acgaaatctt caactacatg gaaaaattcg ccggctacgg tttcaacaaa tcccacgccg
     9001 ccgcctacgc cctgatttcc taccagaccg catggctcaa agcccactac cccgccgaat
     9061 ttatggcggc gaccatgtcg tccgaattgg acaacaccga ccagctcaag catttctacg
     9121 acgactgccg tgccaacggc atcgagttcc tgccgcccga catcaacgaa tccgactacc
     9181 gcttcacgcc gtatccagac atgaaaatcc gctacgcact cggcgcgatt aaaggcacgg
     9241 gcgaagccgc cgtcgaatcc atcatcgccg cgcggcaaag cggcggcaag tttaccggcc
     9301 tcttggactt ctgcgagcgc gtcggcaaag aacacatgaa ccgccgcacc ctcgaggccc
     9361 tgatacgcgg cggcgcgttc gacagcatcg aacccaaccg cgccatgctc ttggcgaaca
     9421 tcgacctcgc tatggacaac gccgaccaaa aagccgccaa cgccaatcag ggcgggcttt
     9481 tcgacatgat ggaagacgcc atcgaaccgg tgcggctcat cgacgcgccg atgtggagcg
     9541 aatcggaaaa actcgccgaa gaaaaaaccg tcatcggctt ttacctgtcc ggccacccgt
     9601 tcggcccgta tgcccaagaa gtccgccaaa tcgccccgca gaaactgtcg aaactcaaac
     9661 cgcaagacag cgtgcgcctc gccggattcg ttaccgccgt acgcaccatg atgggcaagc
     9721 gcggcaaaat cgccttcgtc agccttgaag atttgagcgg acaggttgaa atcatggtcg
     9781 gcggtcagac gttggaaaac tgcgccgact gcctcaaagc cgaccaagtg ctgattatcg
     9841 aatccaaagt cagccgcgac gactacggcg gcggcgacgg gctgcgtatt ctggcaaacc
     9901 aagtcatgac cctgcaaatg gcgcgcgaac gctacgcccg aagcctcagc ctcgccctcg
     9961 ccccgcatca cgacatcggc aaactggtac aactgctcgc cgcccaccaa ctgcccgaca
    10021 cgccgcgcat cccgctgcaa ctgtcgtatg ccaacgaaaa agcgtcgggc aggcttcaag
    10081 tgccgccgaa atggacggtt acgccgagtt ccgcattgtt cggcgaactg gaaacattgc
    10141 tcggcagccg gtcggtgcgc gtcaactggt aacccaaaat ataaatgccg tctgaagccc
    10201 aaaaaccggt ttcattcgta ctttattcga atgattgaat aaaagtaact gccaagaaaa
    10261 acgtattttt tggttatttc gccagtctaa atagagcaac cgggacgatt gatatccgtg
    10321 tgcatgacac agacagcacc aaagggaaaa acggcatttt ccaaagtatc ggtatcaaaa
    10381 ccgccctttc actccaaaaa taccaaatcg acaaaccggg caaagaaatc agaccgtgcc
    10441 gcctgaaaaa acgcccaccc gtccgttaaa ttaacctatc cctgtttcag acggcctgaa
    10501 gcagggattt ttatatcaaa ataaaatgag aaagggagca ataaccctta ggtagctctt
    10561 gttattttcc gatgcaaaac aaagcagtca tatatttaat tccccctacc tctgccaagc
    10621 cttcctcaaa tattcgacgc aatcggtcag cgagtagaac gagacattgc cgtggtcggc
    10681 attcggatat tcccggaaaa agacggcggc cccgtgcctg tccaactctg ccgccatgcg
    10741 ttcggacagt cctgccatat cgcgttcttc cctgcgtttg caaccgctac cccgttcgag
    10801 cgcgccgatg ttgaggcaga catcgatgcc gtttagccgg ttttcagacg gcataaaatc
    10861 gagtatccgc ctgttgtgcc accaaatcga tggggatacg agccaatgcc gtctgaaacg
    10921 gcggtgggaa agcagggaat acagtccgaa cagtgcgccg aacgagtgtc cgaatacggc
    10981 ggtttcattg cggttgaggg tgtagcggct ttctaaaaag gcggtcagct cgctgtcgat
    11041 aaaggcggag aagcggtctg cctgtccgaa ctgctgccgt tcgtctgctg tggcgttgtc
    11101 tccaagcggc ggcgtgtagt cggcggcacg ttgtgccaaa tcgcgcacac tgcctgtcgt
    11161 gtagccgata ccgacaatca ggcagggggc gttgcttcgg gtaacggggt tgggcatcag
    11221 cgactgcatg atgttgaaaa gtgcggggaa aaaggcttcg ccgtccaaaa cgaagaggac
    11281 gggatagcct tcagacggca tttcgccgag tgtcgccgtc tgaatccgat agattcgccc
    11341 cgtgcaggtg gatttgattt cggtttcaaa ggcttggggc agtatggcag gttggaatgt
    11401 gtcggtcggt atgggtttca tgatgttcgg cttgtgggtc agactggcgg caagcggtaa
    11461 aaccgaaccg gcaaagcggt ctgcctgccg gttcggtctg ttttccttcg caatgccata
    11521 ctccagttgt gagagcatag ggtatgcagc gcagcttgtt gtggtttgat gatgctgcgg
    11581 ctgccgtttc agacggcata ttggtcttta aaaactgtaa cgcaggtttg ccgtcaggct
    11641 gcgctccgaa ccgggaatgt taaaggtgtt cgcgccgccg acgcgggcgt agtaatggcg
    11701 gttgaagatg ttgtcggcgt tgatttgcag cttcagcttg ggcgtgaagc ggtatgccgc
    11761 catcgcatcg aacgtggcat aaccgcctgc atgcatccct gcagatgaag taatgccgct
    11821 catcgcgttc actccgccgc cgatggtcag ctcgggcgta acttggtaag tcgtccacag
    11881 gtttgcgctg tgtttgggca tcaataggaa gatgccgtcg tcgcgtgaat tggaggcggt
    11941 tttgatttgg ctgtgcagat agctgtaacc tgcatggatt tgccatttcg gtgtaaccgc
    12001 gccgctgatt tcggtctcaa cgccttccat cacgcgtttg cccaatgcgg cgtaacgggt
    12061 ttttttgttg ttcgggttca acggtgcggc ggcgtttttg tctttcatgc ggtagaacga
    12121 aactcgggca ttgaggcggt cgtccatgta gctgcctttg taaccgactt caaactggtt
    12181 gccttggcgc ggtttgagca gcttgccgtc ggcatcgagg ttggtttgcg gtgtgtagag
    12241 ctgggacagg ctcaggtaga ggctgttgtt gtcgttcaag tcgtaaaccg cgcctgcgta
    12301 gccggtgaac ttggttttgc tggctttgtg cagggttttg ccttcgcccg actcgatttt
    12361 atagtgtccc aaacgtccgc cgccgataag cgacagcccg tcggcaggat ggaaggtgga
    12421 tttgccgtaa atgccgaatt cgtcgaggtt ttcggtagcg acggtatggc tgtaaccgcg
    12481 cacgcctttg cgcgcgttgg caatcaaatc aacctgcggt atgctgcgga actcgtttaa
    12541 agccaggccg ccgcgtgcat ataaagtagt acggccttgt tcgttggtgc tgcggaagcg
    12601 gttgtaatcg gcgccgatga caaattcgtt ggccgtattg cccaagcgga aaggcctgct
    12661 gtagctggcg tcaaacgcga gggctttttg tttgatttct gtacccaaac ccaccgcgca
    12721 ggctttgtcg tcagccgtat tgcagcccgg gcggcctgcc ggggttttca tgcccagctt
    12781 gctgccggca aaggcatagt tggagtcggc atcgcggtcg gaatagcgca taccgacttt
    12841 gccgtagccg ccgttgccga agtaatgttt caaatcggcg aacacgtcgt ggctgtgcat
    12901 tttaaatttg ttccaatccg cgccgacaaa tacgtgttgc ggcagggacg gtaatttgtt
    12961 attggcatcg gctggcaagc cgttgtacgg cgcgaggtgg cgttgctggt aaagatagcc
    13021 cgcgcccaaa accgtatcgg ggttgatgtc ccaatccgcc gccgcgtaga aggtttcgtg
    13081 ccggttgttt ttctcggcgg gacgcggaga cgcgccgacg gtctgcgcca tcacgcggcc
    13141 gcgcacgctg ccgtctgaat tgaggctgcc cgatacgtcc gcctcggctt tatattgttt
    13201 gtgcgtaccg aaccctgccg cagcatgacc ttggaacgct ttggccgggc gtttgcacac
    13261 cagatttacg atgccgccca tctcgccgct gctgtcgaac aagccgctcg gcccgcgcat
    13321 cacttccaca cggtcgaagg caaacagatt gggcagcgtg ccgttgatac tctgcatctg
    13381 cgcgggcagg ccgtcgatgt tgtattcgct gtattcgtaa ccgcgcgcgt aaaccgaaga
    13441 gcgtccgtcg tcgttgctca acacgcgcag gccgggcgtt ttgcgtgcca actggtcaaa
    13501 cgtatcaaca ttgcggtctt tgacctgttg attggtgatg atgctgacag attgcggaat
    13561 ttcgcgcaaa gaagcgggga tttttgtgcc gacggtggcg gcaaacgagc tgtaatcgcc
    13621 gtttttctcg gtggcaatcg cgttgtacga acgctgaccc ttaatatgga cggtttccaa
    13681 atccgccgtt tgtgcggcaa aaaccgaaga cgaaagtgct gccaaaaccg tggcggcggt
    13741 catattgatg cggaaaactg acataaactg tcccattcac ataaatgata atggttctat
    13801 tttaataaag cacaacgcgg cttgttcgga aaaacatatc gcgcagccga caaattttgt
    13861 cgaaaatgcg acacgtctgc gttttccgca taaaatctga cttttcaccg taaacaacct
    13921 gccatgacca cgcccaaact catcatcttc gactgggacg gcacgcttgc cgatacgacc
    13981 caacccatca tcgacaccat gcgccgcagc ttcgccgaat gcggttttcc gccgcccgaa
    14041 gcggaacgcg tccgcagcct gattggctac agcctgcccg aaatcatccg cgacctgctc
    14101 gaaatgccgt ctgaaaccgc cattgccgac atcacacgca cttattccgc acattacctc
    14161 aatcccaaca accgcaatat gacgctcttt cccgatgccc tgccctgtct ggacaagctc
    14221 aaagcacaag gatactggct tgccgtcgcc acgggcaaag ggcgggcggg tttggacaac
    14281 gccatcagcc aaaccgccac cggcggctat tggctcgcca ccgcctgcgc gggggaatat
    14341 ccctccaaac cctcgcccga aatggtattc ggaatctgcg gcgaactggg gttcgacccg
    14401 aaagaggcat tggtcgtcgg cgatacggcg catgacctgc atatggcggc aaacgcaggc
    14461 gcggcggcag tcggcgttgc caccggcgca cattcgcgcg aacagctcct tagcgcaccg
    14521 catctcgccg tattggacgg tttgtccgaa ctgcccggtt ttcttgcaca gcattacgcc
    14581 tgattggttt ccgcatccgg cactcgaaac aaatgccgtc tgaaggttgt tcagacggca
    14641 tttgttttgc ccaaacattc aacgcttgct caacgtttgc acaaatcggg tttggtttcg
    14701 ccctcgcggc gcaactcttt gggcaggacg aacacgatgc tttcttccgc gccctcccct
    14761 tcgcgtacgg tttcatgccc ccacccgcgt atggttgcca atacttcctg caccaacact
    14821 tcgggcgcgg acgcgcctgc cgttacgccg actttgttct tgccctcaaa ccacgcgcgt
    14881 tgcaggtagc ctgcattgtc caccatatac gcatcggttc cgatgcctgc cgccacttcg
    14941 cgcaggcggt tgctgttgga cgaattgggc gaaccgacca caatcacgat gtcgcactgt
    15001 tctgccaact ctttgacggc ggtttgccgg ttggtcgtcg catagcagat atcttccttg
    15061 tgcggattgc ggatattggg gaaacgcgcg ttcagcgcgg cgatgatgtc tttggtttca
    15121 tcgaccgaga gcgtggtttg gctgacatag gcgagtttgt cggggtttct gacttcgagt
    15181 tttgccacat ctccgaccgt ttcgaccaaa agcattttgc ccggcgcaag ctgccccatc
    15241 gttccttcaa cctcgacgtg ccccttatgc ccgatcatga tgatttcaca gtcttgggca
    15301 tccagtcggg cgacttcctt atgcactttc gtcaccagcg ggcaagtcgc atcaaacacg
    15361 cggaaaccgc gctccgccgc ttcttgccgc accgccttcg atacgccgtg tgccgaataa
    15421 accagtgtcg cgcccggcgg cacttccgcc aagtcttcaa taaacaccgc acctttttca
    15481 cgcaggttgt ccacgacgaa tttgttgtga acgacttcgt ggcgcacata aatcggcgcg
    15541 ccgaattctt ccaaagcacg ttcgacaata ctgattgccc gatccacgcc ggcgcagaag
    15601 ccgcgcggat tggcaaggat gatggttttc tcgttcataa gttttgcatt ccgtgttcag
    15661 acggcattca cgtttttttg ccatctttgc gatggacgat attgtcaagc actgccaata
    15721 ccgcgccgac gcagataaaa ctgtcggcaa tattaaaggc gggataaaac caattttgcc
    15781 aataaaacaa taagaaatcg acgacatgac cgtgtatcag gcgatcgatg acattgccta
    15841 acgcaccgcc gataatcatc gccgcacccg ttttgccgag ggctgcaaac tcatcgcgca
    15901 agatggcgcg taccaaatac gcgctcaccg ccaccgccag caccaaaaaa aagtattttt
    15961 gccagccgcc ctgatcggca aggaagctga acgccgcacc cgggttgtac accagcgtca
    16021 gatcgaaaaa ggaaggaatg acattgacgc gttcccgata ctgaaacgac gacagcaccg
    16081 cccacttcga ccactggtcc agcacgatgg cggcaagtgc caatacccaa tagcgcgttt
    16141 tacttgaaac agatgaagac atatttttca acagccggta aaagagtacc attttacccg
    16201 aaaaccccct ttcctgtacc cgaaacggca aatgccgtaa tcttaaaacc cgtcattccc
    16261 gacaacaccg taatctcgaa acccgtcatt cccgcgtagg cgggaatcca gacctgtccg
    16321 cacagaaact tatcggataa aaacagttgc tcaaacccct cgttctagat tcccactttc
    16381 gtgggaatga cggttcagtt gcgttccaac aacaccgcaa tctcgaaa
//
LOCUS       DAWGJV010000033        15451 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWGJV010000033 DAWGJV010000000
VERSION     DAWGJV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.033"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            307..1461
                     /gene="obgE"
                     /locus_tag="WHQ94_000771"
     CDS             307..1461
                     /gene="obgE"
                     /locus_tag="WHQ94_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ2128091.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPIGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETANPAEEALAIINELRKYDEELYD
                     KPRWLVLNKLDMLDEEEAQARSAAFLEAIGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1544..2593
                     /locus_tag="WHQ94_000772"
     CDS             1544..2593
                     /locus_tag="WHQ94_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2128092.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3281..4702
                     /locus_tag="WHQ94_000773"
     CDS             3281..4702
                     /locus_tag="WHQ94_000773"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ2128093.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPIEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4873..5652
                     /gene="xth"
                     /locus_tag="WHQ94_000774"
     CDS             4873..5652
                     /gene="xth"
                     /locus_tag="WHQ94_000774"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ2128094.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDNPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5639..5983
                     /locus_tag="WHQ94_000775"
     CDS             5639..5983
                     /locus_tag="WHQ94_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128095.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFVLPMSVWAIFMLARM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5994..6524
                     /locus_tag="WHQ94_000776"
     CDS             5994..6524
                     /locus_tag="WHQ94_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2128096.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAVSYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTRKKIANIILVPQHPD
                     IFSDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6753..7868)
                     /gene="asd"
                     /locus_tag="WHQ94_000777"
     CDS             complement(6753..7868)
                     /gene="asd"
                     /locus_tag="WHQ94_000777"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ2128097.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            8044..8625
                     /locus_tag="WHQ94_000778"
     CDS             8044..8625
                     /locus_tag="WHQ94_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ2128098.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8687..9541)
                     /gene="folD"
                     /locus_tag="WHQ94_000779"
     CDS             complement(8687..9541)
                     /gene="folD"
                     /locus_tag="WHQ94_000779"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ2128099.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVKFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9764..9841
                     /locus_tag="WHQ94_000780"
     tRNA            9764..9841
                     /locus_tag="WHQ94_000780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9799..9801,aa:Pro,seq:tgg)
     gene            9866..9942
                     /locus_tag="WHQ94_000781"
     tRNA            9866..9942
                     /locus_tag="WHQ94_000781"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9900..9902,aa:Arg,seq:tct)
     gene            9970..10045
                     /locus_tag="WHQ94_000782"
     tRNA            9970..10045
                     /locus_tag="WHQ94_000782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10003..10005,aa:His,seq:gtg)
     gene            10256..10762
                     /gene="rfaE2"
                     /locus_tag="WHQ94_000783"
     CDS             10256..10762
                     /gene="rfaE2"
                     /locus_tag="WHQ94_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEZ2128100.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10759..12537
                     /locus_tag="WHQ94_000784"
     CDS             10759..12537
                     /locus_tag="WHQ94_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ2128101.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12650..13474
                     /locus_tag="WHQ94_000785"
     CDS             12650..13474
                     /locus_tag="WHQ94_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ2128102.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14237..15025)
                     /locus_tag="WHQ94_000786"
     CDS             complement(14237..15025)
                     /locus_tag="WHQ94_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ2128103.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15239..15433)
                     /gene="thiS"
                     /locus_tag="WHQ94_000787"
     CDS             complement(15239..15433)
                     /gene="thiS"
                     /locus_tag="WHQ94_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ2128104.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3677 a         3931 c         4310 g         3533 t
ORIGIN      
        1 cgagtttatc ctagttatct aggacagccc caaaacttat tatatgtaac ggtttgaacg
       61 gacattccgt cgggtcggaa tgtcaaaaag gcgcgattgt accaaagaag ttggacataa
      121 ttttgtttgc aggctgaaga tttgcttaaa aattcattaa gatgggcgga acaaatagtt
      181 tgggtacggc gtgttgaaat accgcttatc ccttataata gcgtccgaaa ttctgttcgg
      241 acggcatcaa aacacacggt aatccgtctg aaccccccat ttgaaaccaa caaacaagag
      301 cattgaatga aattcatcga cgaagcaaaa atcgaagtcg ccgcaggcaa aggcggtaat
      361 ggcgcaacca gtttccgccg cgaaaaattc gtaccgcgcg gcggcccgga cggcggcgac
      421 ggcggcaaag gcggtagcgt ctgggcggaa gccgacgaaa acaccaacac cctcgtcgaa
      481 taccgcttcg ttaaacgcta ccaagccaaa aacggcgaaa aaggccacgg ctccgatcgt
      541 tacggcgcag gtgcggacga catcgtcctc aaaatgccca tcggcaccct tatccgcgac
      601 ctcgacaccg acgaaatcgt tgccgacctc acctaccacg gtcagcgcgt ctgccttgcc
      661 aaaggcggca aaggcggctt gggcaacatc cacttcaaat cctccgtcaa ccgcgcccca
      721 aaacaatcca cgcccggcga agaaggcgaa acccgctccc tgcaactcga actcaaagtc
      781 ctcgccgatg tcggcttatt gggtatgccc aacgccggca aatccaccct gattaccgcc
      841 gtatccgccg cacgccccaa aatcgccaac taccccttca ccaccctgca tccaaactta
      901 ggcgtggtac gcatcgacga aaaccacagc ttcgtgatgg ctgacatccc cggcctgatt
      961 gaaggcgcgg cagaaggcgc gggtttgggg catcgtttcc tcaaacactt atcgcgcacc
     1021 ggattattgc tgcacgtcgt cgatttggca cccttcgacg aaaccgccaa ccccgccgaa
     1081 gaagccctcg ccatcatcaa cgaattgcgc aaatacgacg aagagctcta cgacaaacca
     1141 cgctggctgg tgctgaacaa actcgatatg ctcgatgaag aagaagccca agcgcgtagc
     1201 gccgccttcc tcgaagccat cggctgggac tacccgaaac ccgacgaccg tttccaattt
     1261 gacatggaaa ccccgcgcct cttccaaatc agcgcgctga cccaccaagg cacgcaggaa
     1321 ttggtacacc aaatcaacca atacctgacc gagaaaaaac gtatcgaagc tgaaaaagcc
     1381 gaagcggaga aagcagcggc aaatgttgaa attatcgaac agcagcctaa aacggatacg
     1441 ggcgtgttta agccggagtg aaaaaagaaa gggtttcaga cgatatttgt tctacaaaag
     1501 gttgtttgaa acaattttaa agcaagattg aaatggatta aaaatgtcat ctaaccaata
     1561 tatccagtcg cttgagttga caaactttac cgttcttcca aacgatactt ttgaattttc
     1621 tgaaaatctc aatgttattg ttgccgaaaa cggttgtggc aaaacacatt tgttaaaaat
     1681 tttatacagc ctgctagaag taacatctaa caccaaaaac agattactga aaacagaatt
     1741 gcaaaaaagt tttgccgata aattactgaa tgttttccgt cccgattcct tgggcaggtt
     1801 gagcaaacgt ttgcaggggc gcgggcgtac ggaaatcgtt ttaaaactgc aaaacggcac
     1861 tacgcatagt agtctgaatt tttccagtaa ctcatcatcg caagtcaatg ttcaaagcat
     1921 cggattgaaa gaaaatgaat atactccgac acctatcttc ttacccagcc gcgaattaat
     1981 tacactttgc ccttggttca cttctttgta tcaaaaccaa agcatccctt tcgaagaaac
     2041 ttggtttgat acctgtatgc aattaaatca cccgcttgcc aaaggcccca gagaaacaaa
     2101 aattcgagaa ttgctcgaac ctattgagaa cgcgatgggc ggaaaagtat ctgaagaaca
     2161 aggaaggttt tatctatcgc tgacaaatac gggcggtaag attgaagcac ctttggtagc
     2221 agagggtttg cggaaatttg tcatgattgc ccggttaatc gcaacagggg cattgctgga
     2281 taaaggctat ctattttggg atgaaccgga agctaatctg aatcctaaat tgatcaaagt
     2341 ggctgcccgt attatttggt ctttatctca acagggtatt caggttttta ttgcaacaca
     2401 ctctttattc ttattgagag aattggaact tttgaagctt gaaaataaca cggatttacc
     2461 tgtacgtttt ttctcgttga ttgcttccga tgatggaaca aaagtggagc aagggaacag
     2521 tattaatgat ttgaacacat tgatattgtt agatgaaaac attatgcagg cagacaggta
     2581 tctgaatatt tgaggttaag ctatgactac aatcacagaa aagagattaa cctttgcttt
     2641 tcctgaagat tattacgtta ccaagtatga cgaatgggaa cattataaaa tttttcagaa
     2701 cagttgtaat ttacgtaata aaattgatac aaatgaaaag ggaaagaacg ggataaatca
     2761 aagcgtagat gacgataatg gcagctcagg tgtagatata atagcccttc atgagtcgac
     2821 actttggcta atcgaaatta aagattatta ccgacttggg cttgagccaa atgcccaatc
     2881 aatagatgaa aaactttctg acctaccata tttaatcgca agaaaaattc gagattcatt
     2941 ggcaggattg gtttctgcta aatttaaagc agaaaaacag gaagaaaaag atttttctcg
     3001 cttggcttta gattgtaacg aaataaaaat agtccttcat attgaaatgc ctagtataag
     3061 aagtaaactt tatccctcgt catcagattt agcaaactta ttaaaagata agtttaaatt
     3121 atcagaattt acaaaaaatt ttgcaaactg ttatgcagaa ccaattttca caaatatcag
     3181 ccatattaat aatccgcaac tacgtaatgt tccttggtcc gtatctacag gaacagaaca
     3241 gaaattatca tccgagcaac aaagattaat ccataatccc atgaccacca tctacaacac
     3301 ccttacccgc caaaaagaac cctttgcccc catcgaccct gaaaacgtgc gtatgtacgt
     3361 ttgcggcatg actgtttacg actattgcca cttaggccat gcccgtgtga tggtggtgtt
     3421 cgacatgatt gcccgttggt tgcgcgagtg cggttatccg ctcacttatg tgcgcaatat
     3481 caccgacatc gacgacaaaa tcatcgcccg tgcggctgaa aacggcgaaa ccatcggcga
     3541 gctgactgcg cgtttcattc aggctatgca cgaagatgcc gatgctttgg gtgtgttgcg
     3601 tccggatatt gagccgaagg caacagaaaa cattccgcag atgattgcca tgattgagac
     3661 cctgattcaa aacggcaaag cctatcctgc cgcaaacggc gacgtttact acgccgtgcg
     3721 cgagttttcg gcttacggac aattatcggg caaatcattg gacgacctgc gcgcgggcga
     3781 gcgcgtggaa gtggacggtt tcaaacgcga tccgcttgat tttgtattgt ggaaagcggc
     3841 gaaagcaggc gaaccggcgt gggaaagccc gtggggcaac ggccgtccgg gttggcacat
     3901 tgaatgctct gccatgagtg aaaacctgtt cggcgatacc ttcgacatcc acggcggcgg
     3961 cgcggatttg cagtttccgc accacgaaaa cgaaattgcc caaagcgtcg gcgcgacggg
     4021 gcatacctgc ggtcatcatc acgcgcaaac ccaccacggg caaagcatcg ccagccacgt
     4081 caaatattgg ctgcacaacg gctttatccg cgtggacggc gaaaaaatgt ccaaatcact
     4141 gggcaacttc ttcaccatcc gcgaagtgtt gaaacaatac gaccccgaag tcgtgcgctt
     4201 cttcatcctg cgcgcccact accgcagccc gttgaactat tccgacgcgc atttggacga
     4261 tgcaaaaggt gcgctgaccc gtctgtacac caccttgaaa aacacgccgg cggctgagtt
     4321 tgatttatcc gaaaacgcca acggctacac ccgccgcttc tacgccgcca tgaacgacga
     4381 tttcggtacg gtcgaagcgg ttgccgtgtt gttcgaactg gcaggcgaag tgaacaaaac
     4441 caatgacgca cagctcgccg gctgcctgaa agccttgggc ggcatcatcg gactgctgca
     4501 acgcgatccg attgagttcc tgcaaggagg cgcggcttca gacggcctct ccaacgaaga
     4561 aatcgaagac ttaatcgcac ggcgcaaaca agcgcgcgcg gataaaaact gggcagagtc
     4621 cgaccgcatc cgcgaccttc tgaacgaaca caaaatcatt ctggaagaca acgccggcgg
     4681 tacgacttgg cggcgcggtt gaactgtatc ggaaagggca aaagtaccgc cttttgctct
     4741 ttttccgttt tcagacggca tttcccgctt gaagccccgc cgttttcagc aggcagggcc
     4801 gaatgggaaa tgccgggcgg cgcaccgggt gctgctttgt gtatgggaac attgtgagaa
     4861 cttattttta ttatgcttaa aatcatttcc gccaacgtca acggcatccg ctccgcctac
     4921 aaaaaaggtt tttacgaata tatcgccgca tcgggcgcgg acattgtctg cgtgcaggaa
     4981 ctcaaagcgc aggaggctga tttgtctgcc gatatgaaaa atccgcacgg gatgcacggc
     5041 tattggcatt gcgccgagaa gcgtggttac agcggcgtgg cggtgtacag caaacgcaaa
     5101 cccgacaatg tgcaaatcgg tatgggcatt gaagaattcg acagggaagg gcggtttgtg
     5161 cgttgcgatt tcggcaggtt gagcgttatt tcgctttatt tgcccagcgg cagcagcgca
     5221 gaagaacgcc agcaggtgaa ataccgtttc ctcgatgcgt tttaccctat gctcgaagcg
     5281 atgaaaaacg aagggcgcga cattgtcgtc tgcggcgact ggaacatcgc ccaccaaaac
     5341 atcgacctga aaaactggaa gggcaatcag aaaaattcgg gcttcctgcc cgaagagcgc
     5401 gagtggatag gcaaggtcat ccatacgctg ggctggacgg atatgtggcg cacgctttat
     5461 cccgacaatc cgggctacac ttggtggagc aaccgcggac aggcgtatgc gaaagatgtc
     5521 gggtggcgca tcgattatca gatggttacg cccgaacttg ccgccaaagc cgtgtccgca
     5581 catgtttata aagacgaaaa attttccgac catgcgccgc tggtcgtgga gtatgactat
     5641 gctgccgaat aaagttttgg gtaaatatga ttggaatgtg gacggaaaaa caggaatagg
     5701 cgcggcatgg gtcgtggcgg catttgtgct gcctatgtcg gtgtgggcga tatttatgct
     5761 ggcgcggatg cagggctggc ttgcgccgac caaggcaaac ccgatatggg cgttggtgtg
     5821 gctgctgatt tgcctgccct gcctgctgat tgcgtccaaa tgtttgggtt ggaaaggctg
     5881 gcggcgggtg gtgaatattt ttgtctgcct gaccgtctgc gccatattga gcgtccccgc
     5941 ttcgctgctc atcgccttta ccctccggga cctgctgaaa taggcgcggc ggtatgcagg
     6001 gtttcgaact cgaagatttg acgctttggc tgatacgcga tgccggagaa gatcagatgt
     6061 ggatagaccg ttgggcggtc agttatcccg tcgtgcagat gtccgaagca tcggccggtc
     6121 aaagcatagg ggaatggcag gcagggcttc agacggcatt cgaacggata cagggcaggc
     6181 atatcgccgt tgtcgcacac ggcgcgggtg cggccgcatt cttggcgtgg ctgtaccgcg
     6241 cagacatcct gacacggaag aaaattgcca acatcatcct tgtaccgcag catcccgata
     6301 ttttctccga cgatgcggaa cacactttcc aacgcgtccg ctgtccctgc cgtgccgcat
     6361 tggtggtgtc cgaacacggg gatgtgccgc gagattgggc gcaaaaacag gcggatacgt
     6421 ggaacgcgcg cctgttggtt tccccgcatt cgggcagttt gaacggtatg ctcggcggct
     6481 ggcagtgggg tatgaagctg atgcaggaaa tgttgctggc gtgaacgctt gccttatcgg
     6541 aatttcggct gtagcaggaa gtccgcatat cgggctgttt tcattgacgt gcaggcgcat
     6601 caggttcgga taaagtcggg ctgccgcata ccgccggaaa tggaatcctt tattttttac
     6661 gtcggcagtg ccggcagggc gttatccggc agggttgcaa ggaaaatgcc gtctgaaaca
     6721 ggcttcagac ggcatttcca ttcaaacagg gcttacaggc tgcccaacac gatacgcaat
     6781 acgcggcgca gcggctcggc cgcgccccac aagagctggt cgccgacggt gaacgcgctg
     6841 atgtattcgc cgcccatacc cagtttgcgg atgcgtccga cggggacgga cagcgtgccg
     6901 gtaactttgg cgggggtcag ctcgcggatg ctggcttctt tttcgttggg gatgactttc
     6961 acccagtcgt tcgcgcctgc caaaatcgct tcgatttcgg aaacgggcag gtcttttttc
     7021 aacttcagag tgatggcttg gctgtggcag cgcatcgcgc cgacgcggac gcacaggccg
     7081 tcaatcacgg ttggattgtc gctgcggccg aggattttgt tggtttccac gccgcctttc
     7141 cattcttctt tggactggcc gttgcccaaa tccacgtcaa tccacggaat caggctgccg
     7201 gcgagcggta cgccgaagtt ggctttcgga tagtcttcgc tgcgcaggaa atcggatact
     7261 ttgcggtcga tgtcgagaat cgagccggca ggatcggcaa gttcgtccgc cacttgggcg
     7321 tgaaccgcgc ccataccgct gatgagttcg cgcatgtttt tcgcacctgc gccggaagcg
     7381 gcttggtagg tcatgctggt tgcccattcg accaaatcgt tttggaacag gccgcccaaa
     7441 gccatcagca tcagggagac ggtgcagttg ccgccgatgt agtttttcac gccgtttttg
     7501 aggccgttgt cgataacgtt gcggttgaca gggtcgagga cgataatcgc gtcgtctttc
     7561 atgcgcagag aagatgccgc gtcaacccag tagccgttcc agccgctgtc gcgcagggct
     7621 tggaacacgg atttggtgta atcgccgccc tgacaggtaa cgatgatgtc catttttgcc
     7681 aattcggcaa catcgttggc atccaacaat gttttggctg cctgaccgaa atcaggggct
     7741 gcgccgccga cgttggaagt ggtaaagaaa aacgcttcgg gaatatgggc gaagtcgttt
     7801 tcttcttgca tacgctgcat caaaacagag ccgaccatgc cgcgccagcc gacgaaacct
     7861 actttcattg tgttgctcct tgaggaaaaa tcgttaacaa aattatgtat ggcgtaaagg
     7921 aagcgtttta acgccgtatt gggaaaatgt aaagttcttt atcgggattt ctttaagtat
     7981 tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt aagaacaatt atcaggaacg
     8041 gatatggaag aaaaaaacga ctacaccgac gcggcatccg acaaccgcaa cgggcaggaa
     8101 attgaagtcg gaattgccgg cgcaggcgac cggatacttg ccgcgctgct gaaccaattg
     8161 tttacctttt tggttttgtt ggtgccgttt gccggactga ttgctttcgc catcaagaat
     8221 gaaggcagga ttggcggcag tgaggaaata ttcgggctgc ttttgggcat gacttccttt
     8281 tgggtaggac tggcgggcat tttggtttat accgtcgttc aaatctacta tatgagtcgg
     8341 gacgggcagt cattgggtaa gaagatcatg agaatcaggg tgttgaaaac cgacggccgc
     8401 aatccgggtt ttgtcggcac ggttttggta cgcgaaatcg catggtcggt tttggttgcc
     8461 attattgccg ccgttatcgg tcttgcagta ggtgacaacg gagaaaacgc catcaacctg
     8521 ctggcattcc ttgccaactt tgtcctgctc tttatggtca aacgcgaccg ccgcacgctt
     8581 tacgacatac tggcggatac ggttgtcgtc aagctgccca aataaacgga cacggcaaaa
     8641 tgccgtctga aagcctttca gacggcattt ttatcttcag aaccgctcaa gcatcgtgca
     8701 gtgaagccgc gtgcagggtg ttttccatca atgtggcaat cgtcatcgga cccacgccgc
     8761 cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt ttcaaatttc acgtcgccgc
     8821 acaggctgcc gtcgtccaaa cggttgatgc ccacatcgat aacgaccgcg ccgggtttga
     8881 tccattcgcc tttgacaaag ttcggaatgc ccacgccgac caccaaaata tcggctccgg
     8941 caacctcgtc tgccagattt tcggttgcgc tgtggcagac cgttaccgtt gcgcgcgcca
     9001 gcagcagttc caaagcctgc gggcggccga cgatattcga cgcgccgacc acgaccgctt
     9061 ttttcccctt cggatcaatg ccgtaagctt ccaaaagcgt catcacgccc ttgggcgtac
     9121 acgggcgcat cagcggcatt ttgaccgcca gcctgccgac attgtaagga tggaagccgt
     9181 ccacgtcctt atccggcgaa atacgttcca aaaccgcctg gctgtcgagg tgcttcggca
     9241 gcggtagctg aaccagaata ccgtccactt cggaatcggc attcaggcgg tcgaccagtg
     9301 ccagcagttc ttcctgcgat gttgattcgg gcagctcgta agacagtgat ttgatgccgc
     9361 atttttggca ggcagttttc ttgttgcgga cataaaccgc gctggcaggg tcgcctccga
     9421 ccaaaaccac ggccaggcaa ggcatgtgca gattgtcctg ttggcgttgc gccaccgctt
     9481 cggcaaccgc ctgcaggcgt ttttgcgaaa cttctttacc gttgatcagt tgtgccgaca
     9541 tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat tatcgcattt tccgtttgtt
     9601 tttacagcag gatgccgaaa ataaatttgc cctgaaataa aatacggctg tttggacgga
     9661 ttaaatattt tttcaaatca aaaaactata tttttcatct tggtgtcttg aaaataaatg
     9721 ttgacgcatc tttccctgag ttgtatagtt cgcttcttca agtcggggcg tagcgcagcc
     9781 cggttagcgc atctgctttg ggagcagagg gtcgtgagtt cgaatcccac cgccccgacc
     9841 aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa ccggatagag caccgacctt
     9901 ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc caagaatttg attgaaaacc
     9961 atttttatgg tggctgtagc tcagttggta gagccccgga ttgtgattcc ggttgtcgtg
    10021 ggttcgagcc ccatcagcca ccccagataa aggcccgtac ttttcggtac gggtcttttt
    10081 ccgtttgcac gtactgtccg caccgtcgta ccggtgcggc ggtatgccgt ctgaaagccg
    10141 ggccggtcat acggaaaccg cttcagacgg catttgcgcc gccggctttg cctttcgcat
    10201 atgcttctgt atcatgtagg ttttctgtct tttatggttt ttgtcggaga ctttgatggt
    10261 tgacgcttgg tctgttcctg attttgaatc gaaaatctgt ccgcccgagg cgttggcggc
    10321 gcgtttggcg ttgttgccgc gcccgctggt gtttaccaac ggctgtttcg acatcctcca
    10381 cagggggcat gttacttatc tggcgcaggc gcgttcggca ggggcggcgt tggtgctggc
    10441 gttgaatacc gatgcttcgg tgcggcgttt gggcaagggc ggcgaccgcc cgattaatcc
    10501 tttggagaac cgtgccgccg ttgccgccgc gttggaaagt gtggatttgg taacgtggtt
    10561 tgacgaggat acgccggcgg cgttgattga ggcggtcaaa cctgagattt tggtcaaggg
    10621 cggcgattgg gctgcggata agattgtcgg tgcggcagaa acgttggcgc gcggcggtca
    10681 ggtgttttca attccgtttc tgcaccagac ttcgacaacg aagactttgg caaaaatccg
    10741 tgcggcagag ggcggaaaat gacggttttg aagctttcgc actggcgggt gttggcggag
    10801 cttgccgacg gtttgccgca acacgtctcg caactggcgc gtatggcgga tatgaagccg
    10861 cagcagctca acggtttttg gcagcagatg ccggcgcaca tacgcgggct gttgcgccaa
    10921 cacgacggct attggcggct ggtgcgccca ttggcggttt tcgatgccga aggtttgcgc
    10981 gagctgggag aaaggtcggg ttttcagacg gcattgaagc acgagtgcgc gtccagcaac
    11041 gacgagatac tggaattggc gcggattgcg ccggacaagg cgcacaaaac catatgtgtg
    11101 acccacctgc aaagtaaggg cagggggcgg caggggcgga agtggtcgca ccgtttgggc
    11161 gagtgtctga tgttcagttt tggctgggtg tttgaccggc cgcagtatga gttgggttcg
    11221 ctgtcgcctg ttgcggcagt ggcgtgccgg cgcgccttgt cgcgtttggg tttgaaaacg
    11281 caaatcaagt ggccaaacga tttggtcgtc ggacgcgaca aattgggcgg cattctgatt
    11341 gaaacggtca ggacgggcgg caaaacggtt gccgtggtcg gtatcggcat caatttcgtg
    11401 ctgcccaagg aagtggaaaa cgccgcttcc gtgcaatcgc tgtttcagac ggcatcgcgg
    11461 cggggaaatg ccgatgccgc cgtgttgctg gaaacgctgt tggcggaact tgatgcggtg
    11521 ttgttgcaat atgcgcggga cggatttgcg ccttttgtgg cggaatatca ggctgccaac
    11581 cgcgaccacg gcaaggcggt attgctgttg cgcgacggcg aaaccgtgtt tgaaggcacg
    11641 gttaaaggtg tggacggaca aggcgttctg cacttggaaa cggcagaggg cgaacagacg
    11701 gtcgtcagcg gcgaaatcag cctgcggccc gacgacaggc cggtttccgt gccgaagcgg
    11761 cgggattcgg aacgttttct gctgttggac ggcggcaaca gccggctcaa gtgggcgtgg
    11821 gtggaaaacg gcacgttcgc aaccgtcggt agcgcgccgt accgcgattt gtcgcctttg
    11881 ggcgcggagt gggcggaaaa ggcggatgga aatgtccgca tcgtcggttg cgccgtgtgc
    11941 ggagaattca aaaaggcaca agtgcaggaa cagctcgccc gaaaaatcga gtggctgccg
    12001 tcttccgcac aggctttggg catacgcaac cactaccgcc accccgaaga acacggttcc
    12061 gaccgctggt tcaacgcctt gggcagccgc cgcttcagcc gcaacgcctg cgtcgtcgtc
    12121 agttgcggca cggcggtaac ggttgacgcg ctcaccgatg acggacatta tctcggggga
    12181 accatcatgc ctggtttcca cctgatgaaa gaatcgctcg ccgtccgaac cgccaacctc
    12241 aaccggcacg ccggtaagcg ttatcctttc ccgactacaa cgggcaatgc cgtcgccagc
    12301 ggcatgatgg atgcggtttg cggctcggtt atgatgatgc acgggcgttt gaaagaaaaa
    12361 accggggcgg gcaagcctgt cgatgtcatc attaccggcg gcggcgcggc aaaagttgcc
    12421 gaagccctgc cgcctgcatt tttggcggaa aataccgtgc gcgtggcgga caacctcgtc
    12481 attcacgggc tgctgaacct gattgccgcc gaaggcgggg aatcggaaca tacttaaaaa
    12541 atacaccctt tttcaacacg gggcggtatc cgtccgaatc cgtccggatt ctgccgcccg
    12601 acagactgcc gcccaggcgg gcggggtttc aaacattgag gaaattctaa tgaaatggct
    12661 cttcaccctt ttggcggtac tcaatattgc cgtattcggc ggcacggtgg gctataaatt
    12721 ggccgttaaa gcgacgggcg gcgtgccgga aaaccgggcc gtcgaaaaca cgccccccgc
    12781 aacgcccgcc gccgacaatg cggcggcatc cgtggaggat acggcggcac tgctcaaacc
    12841 gggcgacatc ctgaccgaag agcaggccga gcagttgcgc ttgaaaaagg aagcggaaca
    12901 gaaaaaactg agggagaaaa aacagcgtga agaaaaagcc cgccgcgaaa aactcgccgc
    12961 cgaaaaggcg caggcggaac gcgaaaacgg cgcggcggat gccttatgcg ccgcgcaggc
    13021 aagcctcacg atggacgaag atgactacca ccgcatcaaa ggacttttgg gcaaatggtc
    13081 gcacgttgcc agcaggagcg tcgaaaaacg caccgccaaa gccaaacctg ccgacaaaac
    13141 ctaccgcgtc gtcctgcccg tttccgccga tgccgaaaat caggcggcgg agctgtctgc
    13201 caaaggtttc aaccccatac cgtttgacgg cgcattgagt ttgggtgtcg gcaacagccg
    13261 ggaaaacgcc caagccctgc aaaaccggct tgccggcgcc ggattcggcg gggcgcatat
    13321 tgtcgaacac tttgccgaag ccgacaggca ggacgattct ttgagcgtgt cgcgtatgac
    13381 ggttttgttt accggcgtga atgccgccga tgcggacgaa attcgtaaaa tcacgtccct
    13441 atacggcaaa ctgaacctca agtcttgcaa ataggcgcga aagccggacg ataaacgcca
    13501 aacccaccgc gaaggtgggt ttggcgtttc ggttttgagt atctgatata taaaaaggga
    13561 atgtatgtct cgtatatgcc gactccaagt gtgaaagtga tgatggggaa atatggtgtt
    13621 taatggttcg gaacggacga cttccgagtg gttttgatta tacgctaata ataataatta
    13681 tcaataaaaa tttaaaaata aatgcggatt ttttgtaatg agatgaaaaa taaacaaatt
    13741 aaactatttg ccgatgtttg tgattcgggt cggcaaactt tcattgtgcc ggacaaatcg
    13801 gggtcggcag gcatagctga gggcttcgtg cctgacggaa ataaggtcgc ccgtgtttga
    13861 acattcgtcc gatttggcgc ggacagggtt gttatttggg ggtgtttgag cgtggattcg
    13921 gggatgcgaa agcggtaaaa ccgttgcgga tgaaggctta cggacggctt taggtatttt
    13981 taagtgtatt caaacaacaa caaaacgctt cttctattgc cgtatttagg gcttgcgcgg
    14041 cagcctgcac accgtggagt ccgggtattt cgttggttgc ggcatcggga cgggcggcaa
    14101 ggttttccga ccggtttttc cattttttcc gacaaagccg tgatgccggg gaatggcggt
    14161 ttgcccgttt cggaccgccg ccgttttgag ccgcgatgcc gtctgaagcc ttcagacggc
    14221 atttttgccg cctttttcaa tattccgccg aatgccaaaa cggctgtccg actgtcggcg
    14281 tgctggcttg cgctttgtcg cgtgcttcga ccggttcggc ttcaaatgcc agccgtccgg
    14341 attcgacggc gagtgcgaag gcgcgtgcca tattgaccgg atcgccgctg cgggaaacgg
    14401 cagtattcaa aagcacgccg tcaaaacccc attccatcac ttgtgccgcc tgtgagggca
    14461 aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag gcgttcgcgc aggacgttca
    14521 acgcgtaggc gtgaaccgcg cccaaacccg tgccgatcgg agccgcccac ggcattaacg
    14581 cctgacagcc cacatcgagc aggcggcggc aggcaatcag gtcttcggtg caataaggca
    14641 gcactttgaa gccgtcttta atcaggattt ccgccgcttc gacaagttgg aacacatccg
    14701 gctgcaaggt gtcgtcgtcg ccgatgagtt cgagtttaat ccaatcggtt tcaaacactt
    14761 cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct ttggcagcct gccgtgttcg
    14821 gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa cccctgaccg tgcgcctcgc
    14881 cgccgcttcc cgtgcggcgc agcgagacgg taatcatcgc gggttgggcg atacggacgg
    14941 attgtttgag gatttcaggg gtcgggtagg cagccgtgcc gagcagcagc cgcgagggga
    15001 aagtttctcc gtatagggtg agcataatgg gttcctttgt atagtggatt aacaaaaacc
    15061 agtacggcgt tgcctcgcct tagctcaaag aaaacgattc tctaaggtgc tgaagcacta
    15121 agtgaatcgg ttccgtacta tccgtactgt ctgcggcttc gtcgccttgt cctgatttaa
    15181 agttaatcca ccataacgtt gttttgggga caggggtcgt ctgaaaaggc aaaaccgcct
    15241 agccgccgac caccggccgc acgatatcga ttttgtcgtt ttcgtttaaa accgtttccg
    15301 catacgcgcc tttggggacg aaaacggtgt tgaccgccac ggcaaagggc ttttgcggcg
    15361 cggtttgggc gatgaggtcg gcaacggtcg tgccgtgaag ttcggcgggt ccgccgttta
    15421 agatgatgtt catggtttct cgatgtttct g
//
LOCUS       DAWGJV010000034        15386 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWGJV010000034 DAWGJV010000000
VERSION     DAWGJV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.034"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(103..1890)
                     /gene="lpdA"
                     /locus_tag="WHQ94_000788"
     CDS             complement(103..1890)
                     /gene="lpdA"
                     /locus_tag="WHQ94_000788"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2128105.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1908..2345)
                     /locus_tag="WHQ94_000789"
     CDS             complement(1908..2345)
                     /locus_tag="WHQ94_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128106.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2439..4046)
                     /gene="aceF"
                     /locus_tag="WHQ94_000790"
     CDS             complement(2439..4046)
                     /gene="aceF"
                     /locus_tag="WHQ94_000790"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ2128107.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LMLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4196..6859)
                     /gene="aceE"
                     /locus_tag="WHQ94_000791"
     CDS             complement(4196..6859)
                     /gene="aceE"
                     /locus_tag="WHQ94_000791"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ2128108.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7554..9266
                     /locus_tag="WHQ94_000792"
     CDS             7554..9266
                     /locus_tag="WHQ94_000792"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ2128109.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVASVTA
                     SLNG"
     gene            9329..9986
                     /locus_tag="WHQ94_000793"
                     /pseudo
     CDS             9329..9986
                     /locus_tag="WHQ94_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            complement(9996..10451)
                     /gene="ruvX"
                     /locus_tag="WHQ94_000794"
     CDS             complement(9996..10451)
                     /gene="ruvX"
                     /locus_tag="WHQ94_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ2128110.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10444..10992)
                     /locus_tag="WHQ94_000795"
     CDS             complement(10444..10992)
                     /locus_tag="WHQ94_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ2128111.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10994..11554)
                     /locus_tag="WHQ94_000796"
     CDS             complement(10994..11554)
                     /locus_tag="WHQ94_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ2128112.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11882..13687
                     /locus_tag="WHQ94_000797"
     CDS             11882..13687
                     /locus_tag="WHQ94_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ2128113.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13807..15291
                     /locus_tag="WHQ94_000798"
     CDS             13807..15291
                     /locus_tag="WHQ94_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ2128114.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELLKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3682 a         3792 c         4258 g         3654 t
ORIGIN      
        1 cggaaaaaat attttaatcc gctgtaaaat agaaaatgcc gtctgaacat ttcagacggc
       61 atttgctcaa ataaacctta tcggctgttt attcagtcgg atttattttt tcttttgcgg
      121 aggcaggtcg gtacaagtac ccaatgccac ttccgccgcc ataccgatgg attcgcccaa
      181 ggtcgggtgc gggtggatgg ttttgccgat gtctgccgcg tcgcagccca tttcgatggc
      241 aaggcagact tcgccgatca tgtcgccacc gttcggaccg acaatgccgc cgccgatgat
      301 gcggccggtt tcggcatcga aaatcagctt ggtaaagccg ttgtcgcaac cgttggcaat
      361 cgcacgaccg gaagccgccc acgggaagtt ggctttggtg attttgcggc cggaggcttt
      421 ggcggacaac tcggtttcgc ccacccacgc cacttcgggg gaagtgtagg caacgcccgg
      481 aatcacgcgc gcgtcgaagt aggctttgtg gccggcgcag ttttcagcgg caacgtggcc
      541 ttcgtgaacg gctttgtgcg ccaacatcgg ttgaccgacg atgtcgccga tggcgtagat
      601 gtgcggcacg ttggtacgca tttgtttgtc cacttcgatg aagccgcgat cggttacggc
      661 aacgcctgct ttttccgcgc tgatgagttt gccgttgggc gcgcggccgg cggcaaccaa
      721 tacggcatcg tagcgttgcg gctctttcgg tgcatttgca ccttcaaagg taacgtaaac
      781 gccgtcttct ttcggctcga ctgcaacggt tttggtgttg accataatgt tgtcaaaacg
      841 gtattcgttt tgtttttgcc atactttcac caagtcgcgg tctgcgcctt gcatcaggcc
      901 gtccatcatt tcaaccacat ccaaacgcga acccagcgtg ctgtaaaccg tacccatctc
      961 gaggccgata atgccgccgc cgataatcag cagtttgccc ggtacttctt tcagagccaa
     1021 tgcgccgctg gaatcgatga tgcgcggatc ttcaggaatg aaaggcagtt tggttacgcg
     1081 gctgcctgct gcaatgatac agtttttgaa ggcaacgatt tttttctcgc cggtagtggc
     1141 tgcctgttcg tacgcgtcgc cggcagtcag cgacacttcc aagtggtgcg ggtctaagaa
     1201 ttgcccgtcg ccttggataa cgtccacttt acggcttttc gccatacctg ccaaaccgcc
     1261 cgtgaggcgg gaaactacgc cgtctttgta ggcgcgaagc atatcgatgt cgagttccgg
     1321 ctcggggtat ttgataccgt tggcagccaa gtggcgcact tcgtcgataa cggcggcatt
     1381 gtgcaacaag gctttggaag ggatacagcc gacgttcagg caaacgccgc ccaaagtttt
     1441 gtaacgttcg acgatggcga ctttcaagcc ttcatcggca gcggcaaatg cagcggagta
     1501 accgccggga ccgccaccca atacgaccac gtcgtactcg gcatcggcag aaccgccgaa
     1561 ttgcgcggct tgcggagcag gagcagcagc tttaggggct tcttgcgccg gggcggcagc
     1621 aggtgcttca gctttgggag cggctgccgt gccttcagct tcaacgacga caatcaagcc
     1681 gccttcggag attttgtcgc cgactttgac tttcacttct ttgaccacgc ccgcaacttc
     1741 ggcaggcacg tccatcgtcg ctttgtcggt ttccagggta atcagggtat cgtcaacggc
     1801 gatggtgtct cctacgttca cttcaactgc gataatatct acattttcgt gtccgccaat
     1861 gtcgggcact ttcaattcaa ctaaagccat tatattgacc tttcaactta gttaatttct
     1921 atcaactctt tagcaataat cacttcttca ggaatacagc cacaatcttc agctctgatt
     1981 gttacttcct tctcatttgg gttttctgga tgttcaacct catattcaaa aatagaatat
     2041 tgaccaaaca aatccctatt taaaacatat atatagccat tttcgatgcc ggaacttgtt
     2101 gcaaatttct tggcaatttc cttgtctgtt gtcgtagata tataacatcc gtcatataga
     2161 cctgtttcaa tttgatgggc gtaaactgca ttcttcacac ttggaccatg tgtagcttta
     2221 ccatcatatt taaacttacc atcataacga attgcaactt cagctttatt acctttaggt
     2281 tttaactgtc cattattttg ctcatcttgt tggcaactaa tgcctctata taagaaattt
     2341 cccataagtt attaatttaa aggttgaatt taacaccatt actcatcaat ctcaagcaat
     2401 ccaaacatta tcagactgct tgagagggat gttttatttt ataaggtaat gcggcggaag
     2461 tctttcaaca gcttcgccaa gaatacggtg aagcgcatac cggccgcacc gtcgatgacg
     2521 cggtggtcga aggacaggct caacgggcac atcaggcgcg gggcaaactc tttgccgttc
     2581 caaaccggtt tgatttggga tttgcacacg cccaagatgg cgacttcggg agcgttcaca
     2641 atcggcgtga agcctgtgcc gccgatgccg cccaagctgg aaatggtaaa gcatgcgcct
     2701 tgcatttctt gtggtttgag cttgccttcg cgggcttttt tggacaattc tgtcagctct
     2761 tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac aaccaagccg
     2821 ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcatcag gttgtcgccg
     2881 tccagagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc tttgatgatg
     2941 aacgccagag gagacagttt cacgccttcg cgttcccatt ctttgttcag ttgtttgcgg
     3001 aattcttcca gctcggtcat atctgcttct tcgtgtacgg taacgtgggg aatcaccacc
     3061 cagttgcgag acaggttttg accggagatt ttcttaatgc gggacaattc tttaacttcg
     3121 acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag actgctgccc
     3181 aaagatgcgc cggctgcggc aggttttgcc gcgccaccct gcattacgga tttcacaaag
     3241 gctttgatgt cgtcgcccac gatacggcct ttcaagccgg tacctttgac ttggcccaaa
     3301 tccacgccca attcgcgcgc cagtttgcgt gcggaagggc cggcgtgtgc tttggcgaaa
     3361 gcggcctcgt cgattttggc agcggcaggt gcagaaggtg caggcgcggc ggcgggtgcg
     3421 gcggcagcag tcggaggcgg agcggcagcc ggtgcggcag cttgagcggc ttgagcaggg
     3481 gctgctgcgg cagagccggc ggtttctact tcgataatgg cagtaccttc ggacactttg
     3541 tcgccgactt ttaagaatac ggctttaacg acaccggcag cggtacaagg tacgtccatc
     3601 gttgctttgt cggtttccaa agtaatcagc gtgtcgtctt cggcaacggt atcgccgact
     3661 ttgatctcga cggcgattac atccacatcg gtatggccgc cgatgtcggg aacggctact
     3721 tgaacggttg caccgcctgc ggcaacgggt gcggcagcag gtgcaggttg tgcttcggca
     3781 gcagcaggag cggcttcggc agcggcggca ccggtttcaa cggtcaaaat tacgccgcct
     3841 tcggagattt tgtcgccgac tttgactttt acttctttca cgacaccggc cgcatcggca
     3901 ggcacatcca tcgtggcttt gtcggtttcc agtgtaatca gggtgtcgtc aacggcgatg
     3961 gtgtcgcccg ctttaacttc tacggcgatg atgtcgacgt tttcgtgacc gccgatatcg
     4021 gggactttga tttctacgat actcatttga gttcctttaa tctttttcaa ttggatgccg
     4081 tctgaaagct gcttcaggcg gcatcggtac ggtttgtctg ctcaaaccat tcgggcattc
     4141 ggttttcaga cggcctgatg tcggaatggg gcaaacaggc cgtctgaaac atcaatcagc
     4201 gtttccagct aggagctgaa tcggctttga tgccgtattt ctcaatggct tgttgaacgg
     4261 tttctttgct gactttgcct tgttccgcca atgcggccaa tgcggccacg gcaacgttgt
     4321 agcgatccac ttcaaagaag cggcgcaggt tggcgcggct gtcggagcgg ccgaagccgt
     4381 cagtacccaa gacgtggtag tcgttcggga tgtaggcgcg gatacggtca gcatagctgc
     4441 ggatatagtc ggtagcggca ataaccggac cgtcatgacc ttgcagttgg gaagtaacga
     4501 aaggtacttt ttcggcttcc agcggatgca ggcggttgaa gcgttctacc tcgacagcgt
     4561 cgcggtgcag caggttgaag gatgggcaag accaaatgtc tgcttccacg ccgaagtcgg
     4621 ctttcagcag ctcggcaccg gcaatcactt cttgcaaaat cgtaccggaa cccatcagtt
     4681 ggactttctt gtcgcctttg ccgccggctt tcagcaggta catacctttc aagatgtctt
     4741 gttccgcacc ttcgggcata tccggatggg tgtagttctc gttcatcagg gtgatgtagt
     4801 agaacacgtc ttcattattg gcatacatac ggcgcagacc gtcttgtacg atgacggcga
     4861 cttcgtattg gaaagtcggg tcataagata cgcagttcgg aatcaggtcg gcctggatgt
     4921 ggctgtggcc gtcttcgtgt tgcaggcctt cgccgttcag cgtcgtacgg ccggcagtac
     4981 cgcccagcag gaagccgcgc gcgtgcatat cgcccgccgc ccaagccaag tcgccgatac
     5041 gttggaaacc gaacatagag tagtagatgt agaacggaat catcgcgaag ttgctgttgg
     5101 cgtagctggt tgcagccgca atccagtcgg ccatcgcgcc cggttcgtta ataccttctt
     5161 gcaagatttg accgtcaacg gattctttat agaacatcag ttggtctttg tcttgagggg
     5221 tatattgctg acctttcgga ttccaaatac cgtattggcg gaacatacct tccatgccga
     5281 aagtacggct ttcgtcggga acgataggta cgacgcgttt gccgattttt ttgtctttca
     5341 gcaaagtgga caggatgcgg acgaatgcca tcgtggtcga gaactcgcgt tcaccgctgg
     5401 atttgagttg tgcgtcgaat gctgacagct cgggcacttc caatacttcc tgcgtcggtt
     5461 tgcgttgcgg caggtagccg cccaaagcat cgcggcgtgc gtgcaggtat ttgtattctt
     5521 ccgtatcggg ggcaaaagtc aggtaaggca gatcgccgct ttcgatttgt tcgtcggtaa
     5581 ccggaatgtc aaagcggtcg cggaattgtt tcagggacgc tttgtccatt tttttggctt
     5641 ggtgggcaac gttctgacct tcgccggatg cgcccatacc gtaaccttta atggttttcg
     5701 ccaagatgac ggtaggtttg ccgtcggcat ggttcgctgc gcggtcgtag gcgttgtaca
     5761 ctttttgcgg gtcgtggccg ccgcggttca atgcccagag ttgctcatcg gtcatatcgg
     5821 caaccaatgc tttcagttcg ggcgtattga agaagtgttc gcgcacatac gcgccgtctt
     5881 tggatttgta agtttggtag tcgccgtcca aacattcttc catacgttgg cgcaggatac
     5941 cgtctttgtc tttcgccaag aggcggtccc agcggcggcc ccaaatgact ttgacgacat
     6001 tccagccggc gccggcaaag ttgccttcca attcttggat gattttgccg ttgccgcgta
     6061 ccggaccgtc caagcgttgc agattgcagt tgatgacgaa aatcaggttg tccaagcctt
     6121 cgcgtgcagc cagtgcgatt gcaccttgag attcgggttc gtccatttcg ccgtcgccgc
     6181 agaaacacca tactttacgg cctttggttt ttgccaaacc acgcgattcc aagtatttca
     6241 ggaaacgcgc ctgataaatc gccatgatgg gccccaagcc catggatacg gtcgggaact
     6301 gccaaaagtc gggcaagagg tgggggtgcg gataggaagg cagaccgtgt ccgtccactt
     6361 cttggcggaa gttgttcagc tggtcttcgg tcagacggcc ctcgacgaat gcgcgtgcat
     6421 agatgcccgg ggcgacgtga ccttggaaga agaccaaatc gccttcttcg ccttcgcctt
     6481 tggctttcca aaagtggttg aaaccgactt cgtacatggt ggcggcagat tggaaagatg
     6541 cgatgtgccc acccagttcc aaatctttct tgccggcgcg caatacgatg gcggcggcgt
     6601 tccagcgcac gaatgcgcgg atgcggtgtt cgatgttttg gtcgcccgga atgccttttt
     6661 cgttttcaac cgaaacggta ttcaaatacg gagtggtcgt gccgtgtggc atacgtacgc
     6721 ccttgtcgcg gcagtatttg accaggtttt ccaagaggta ttgcgcgcgt tcgccgcctt
     6781 catattcgag gacggagctt aacgcgtcca gccactcttg ggtttcgata gggtcaacat
     6841 cgtgtaattg ggtggacata gtatctatcc tttatgttga gtgtttaatc ggcagggagg
     6901 gtgtagccaa cccctttatt ttcgtttgtg aaaaataata agcaaaaacg aaaatttcta
     6961 aatcaaaccg aaaaaaaata atttaagttt aatgttttca aaaaattata aaagttttgg
     7021 aaacaaggga aaacttgaaa aaaatgttcg atctgctcca cacgaaatcc tatcagcaat
     7081 cgggtctgct ggcaaataac catgcctgta aaagcaggtt ttatactgaa gttggcgttt
     7141 ggtaaggtca agtctttttg atttcgaaat attctgtttg attttggcga tattttttcg
     7201 gaattgtggt atatcgaatc attatgtctc tgtcggcaat tttcctattg tagaaaatct
     7261 atacacaagc tttgtggata accgtgttga taagccacgg cttgtgttaa aaaagtctta
     7321 cgcaacaaat gtacgcttat tttgattaaa aaataggcac atttaaatct ataaaaatca
     7381 tgtgcttatg ttcaatatga tgtttctggt catgtttttg ccgtctgtta tgggatttgt
     7441 cccgcgcttg gggcaggggg tgaattttgg aggttggtgc aaaacctgct tttttcgcgt
     7501 tacaatctgc acccgattta cttttgaatt tgcctgtttg gaaaaatact tttatgaaag
     7561 ccagccaatt ctttatctct actttaaaag aagcccctgc cgaagccgcg cttgccagcc
     7621 acaagctgat gattcgcgcc ggtctgatta aagccaacgc gtccggcctt tatacttgga
     7681 tgccgatggg gctgcgcgtg ttacgcaaag tcgaaaacgt cgtgcgcgag gaaatggcgc
     7741 gcgcgggcag cgtggagttg ctgatgccgg tggtgcagcc tgccgagctg tggcaggaat
     7801 ccggccgctg ggagttttac ggtaaagaat tgctgcgcct gaaagaccgc cacgaccgcg
     7861 atttctgcat gggcccgacc tgcgaggaag tcatcgccga catcgtgcgt aaagagatca
     7921 acagctacaa acaactgccg aaaaactttt accacatcca aaccaaattc cgtgacgaag
     7981 tgcgcccgcg cttcggtgtg atgcgtgcgc gcgagtttgt catgaaagat gcttattcct
     8041 tccatgccga ctacgcctcg cttcagacga cctatgatgc catgtatgac gcttactgcc
     8101 gtatcttcac ccgtctgggc ttggcattcc gccccgtcgc cgcagatacc ggcagcatcg
     8161 gcggtaccgg ctctcacgag tttcaagtgt tggcggaaag cggcgaagat gtgattgcat
     8221 acagcgacac ttccgattac gccgccaatg tcgagctggc accgaccttg ccgcttaaag
     8281 gcgaacgcac cgctgctcag gcagaactcg tcaaagtaca cacgccaaac gtcaaaacca
     8341 ttgagtcttt ggttgaattc ctgaatattc cggttgaaca aaccctcaaa tccatcgtgg
     8401 tcgaaggcga aaacgaaggc gaaatcgtct tattactgtt gcgcggcgac catgagttca
     8461 acgacatcaa ggcagaaaaa ctggcgggcg tgaaatcgcc attgacgatg gcaagttctg
     8521 ccgcgattgt cgaacagttc ggcgcaaacg gcggctctct tggtcctgtc ggcttcaaag
     8581 gcaaagtcta tgccgatttc gctaccgaaa aaggcgcgga ctgggtcatc ggtgcgaacg
     8641 aagacggcta ccactatacc ggcttcaact tcggtcgcga tgccgccgag cctgagtttg
     8701 tcgatttgcg caacgtcgtc gaaggcgacg aaagccccga cgggcaaggc cgtctgaaac
     8761 tggcgcgcgg catcgaagtc ggacacgttt tccaattgcg cgacaaatac acccaagcca
     8821 tgaacgtaag cttccttgac aacaacggca aatcgcaaat catggaaatg ggctgctacg
     8881 gtatcggcat cacccgcgtc gtcgccgccg ccatcgagca gaataacgac gaaaaaggca
     8941 tcatctggac caaagcaatg gcgccgtttg aagtcgttat cgtgccgatg aactacaaaa
     9001 aatcagacac cgtacgcgaa gccgccgaca aaatctatgc cgaattgctg gcggcaggcg
     9061 cggatgtgct gctggatgac cgcgacgaac gcgcaggcgt attgctgaac gattccgagc
     9121 tgttgggcat cccgcaccgc atcgtcatcg gcgaccgcgc cttgaaagaa ggcaatgtcg
     9181 aatacgccga acgccgcaac aacgaagcgc aggcagttgc aatcggagaa attgttgcga
     9241 gtgtaacagc ttcattaaat gggtaagctt ggtcaaatgc cgtctgaaaa taatttcaga
     9301 cggcattttt atttggggag aaagtgaagt ggcctcagta tttttagaag gagaagcagt
     9361 cgaggtcggc aatattttct gtatcggcag gaactatgcc gcacatattg aagagttaaa
     9421 aaacgaaata ccttccgaac ctgtggtgtt tatgaagccg tcaggcagca ttttgaacag
     9481 cggcggtaca atcttactgc ccgagttcag tagggatgtg cagtttgagt gcgaactcgt
     9541 gctgctggtc ggtaaggatt cagacggcac gggcgagggt aaagatattt tgggatgtgt
     9601 cgcaggatat ggtgtagggc tggaccttac ggcacgggat atccaatgcc gtctgaagga
     9661 aaaaagggct gccctggctg aaggcaaaag ggttcaggca ttccgcgtgc gtgtcggatt
     9721 ttgcggcggc aggcagaatc ggcaatcccg agaatgtgtt gttttcatta aaacaaaacg
     9781 gcgtattgaa acagcgcggg gataccggtt tgatgattta cccgattcgg gaaattttac
     9841 acaaactggc ggcagattac ggtttgggga aaggggattt ggtgtttacc ggaacgccct
     9901 ccggcgtggg cgcaatcggt gcgggggaca atttggcatt ggagctggac ggcttggtgc
     9961 gcgcctcttt cacggtaggg tgttaaccgc gccgcttaac cctcacgccc gttgaaacat
    10021 tccgccggac cgccctcgaa aaaaccgtgc aagatggctt gcgccgccac ttggtcgagc
    10081 accgatttgc gttttttgcc gaagacctgt gcttccgaaa gcaggctttc ggcatagacg
    10141 gacgacagcc gttcgtcaac ccaatagacg gggagattga acctgccgtt cagcctgcgt
    10201 ccgaacttgc gcgacaggtg cgtcatttca tgtttcgtgc cgtcggtatg cacgggcagt
    10261 ccgacgacaa aataacgcgg ctgccattct tgaaccagct tggcgattgc cgcgaacttt
    10321 tcatcgttgc tgccgccggt aacggtgctc aaaggatggg atagccctaa ttccgcgtct
    10381 ccttgtgcca cgccgatacg cgcttcgccg aagtcgaatg ccagtgccgt tccttttgga
    10441 attttatgca tggccggctc ccgaaaacag ggcgagcggg tcgatgccga gtttggcgaa
    10501 tgcggcggcg taacggtgtt cgtaggggat gtcgaacagg atgtgttcgt cggcgggaac
    10561 agtcagccac gcattgtcgg caagttcgcg ttcgagctgg ccttttcccc agcttgaata
    10621 gcctatgctg atcaaggctt tgtcaaccgc accttcgcgt gaaatatttt caatcacgtc
    10681 tcgggaagaa gttagcgcga tattgtctga aacgccgata ctgctttgcc agttgccgat
    10741 cggggtatgc acgacataac ctcgctcgac ttgcaccggc ccgcccatca tcacgctgtc
    10801 gtgctgcatc cgcatgggga tgtttttgcc ggtggcggaa aaaatcatgt ccatcgtaat
    10861 cggagagggt ttgttgatgg cgatgccgag tgcgccgtct tcatcgtgtt tgcagatata
    10921 gacgaccgat tgtgaaaaaa acgcgtcttc catatcgggc atggcgacga gaaagtggtt
    10981 ggagaggttc atatcagcgt ttgtcgggat tttctatcgg gttggaaatc atgcaggcgc
    11041 cggcaagcag gttgccggaa gtatcggctt gcaccccatc ggtttgcggt atgccgccgc
    11101 cgaaacagga aaccgcgctt aaggaatttt tcggcgagcc ttggatgatt ttatcgctgt
    11161 acaccaaata ggcgaaggtt ttgcgtttgg ggtcgtaata acggacaatc tgccggctct
    11221 tgaacgcgaa gctcgcaccg tgtttgaaaa cttctttcgg tttgcgcacg gcggtttcgt
    11281 caaaagaaat cgaagatgcc gtctgaacgc acgaaaccga tgcgtcggac gcgtcctctt
    11341 ccaaattgac catttccttc aagccgcctt tttttgcata cgaaatataa caggcaaccc
    11401 cttgaacgtc gggatcgtcg aatccttcca cttcgatacg gtcgtttttg cccagtatgt
    11461 tgaaaacggt acttgcccgt ccgattttat cggtttcgcc gctgccgcag gcagtcagca
    11521 ggacggcggc agacagcagt agcagtctgt tcatcaaaat ttccttatcg gttgaatccc
    11581 cgttttccgt gaaggcggca gggcttgctt tgtttggagg ggcgcacgtt cccaaaatcg
    11641 ggcaacatac ggtatgcggc ttaatgtgct accctgtgtg tcggaacaca atatccgttg
    11701 cggcattgaa aatatgatgc acaatataag gctgtttgag ccgattgcaa gtatccgcgc
    11761 aaagaaggcg caagattggg caaacggcat tttcagacgg cataactgac agtataatcc
    11821 aaacgtccgg ctgctttcgt gcagccttca gtattatcca actttttccg aaggtcgaac
    11881 catgcgctac aaaccccttc tgcttgccct gatgctcgtt ttttccacgc ccgccgttgc
    11941 cgcccacgac gcggcacaca accgttccgc cgaagtgaaa aaacagacga agaacaaaaa
    12001 agaacagccc gaagcggcgg aaggcaaaaa agaaaaaggc aaaaatggcg cagtgaaaga
    12061 taaaaaaaca ggcggcaaag aggcggcaaa agagggcaaa gagtccaaaa aaaccgccaa
    12121 aaaccgcaaa gaagcagaga aggaggcgac atccaggcag tctgtgcgca aaggacacga
    12181 aggggataag aaatcgaagg cggaacacaa aaaggcacat ggcaagcccg tgtccggatc
    12241 caaagaaaaa aacgcaaaaa cacagcctga aaacaagcaa ggcaaaaaag aggcaaaagg
    12301 acagggcaat ccgcgcaagg gcggcaaggc ggaaaaagac actgtttctg caaataaaaa
    12361 agtccgttcc gacaagaacg gcaaagcagt gaaacaggac aaaaaataca gggaagagaa
    12421 aaatgccaaa accgattccg acgaattgaa agccgccgtt gccgctgcca ccaatgatgt
    12481 cgaaaacaaa aaagccctgc tcaaacaaag cgaaggaatg ctgcttcatg tcagcaattc
    12541 cctcaaacag cttcaggaag agcgtatccg ccaagaacgt atccgccaag agcgtatccg
    12601 tcaggcgcgc ggcaaccttg cttccgtcaa ccgcaaacag cgcgaggctt gggacaagtt
    12661 ccaaaaactc aataccgagc tgaaccgttt gaaaacggaa gtcgccgcta cgaaagcgca
    12721 gatttcccgt ttcgtatcgg ggaactataa aaacagccag ccgaatgcgg ttgccctgtt
    12781 cctgaaaaac gccgaaccgg gtcagaaaaa ccgctttttg cgttatacgc gttatgtaaa
    12841 cgcctccaat cgggaagttg tcaaggattt ggaaaaacag cagaaggctt tggcggtaca
    12901 agagcagaaa atcaacaatg agcttgcccg tttgaagaaa attcaggcaa acgtgcaatc
    12961 cctgctgaaa aaacagggtg taaccgatgc ggcggaacag acggaaagcc gcagacagaa
    13021 tgccaaaatc gccaaagatg cccgaaaact gctggaacag aaagggaacg agcagcagct
    13081 gaacaagctc ttgagcaatt tggagaagaa aaaggccgaa caccgcattc aggatgcgga
    13141 agcaaaaaga aaattggctg aagccagact ggcggcagcc gaaaaagcca gaaaagaagc
    13201 ggcgcagcag aaggctgaag cacgacgtgc ggaaatgtcc aacctgaccg ccgaagacag
    13261 gaacatccaa gcgccttcgg ttatgggtat cggcagtgcc gacggtttca gccgcatgca
    13321 gggacgtttg aaaaaaccgg ttgacggtgt gccgaccgga cttttcgggc agaaccggag
    13381 cggcggcgat gtttggaaag gcgtgttcta ttccactgca ccggcaacgg ttgaaagcat
    13441 tgcgccggga acggtaagct atgcggacga gttggacggc tacggcaaag tggtcgtggt
    13501 cgatcacggc gagaactaca tcagcatcta tgccggtttg agcgaaattt ccgtcggcaa
    13561 gggttatatg gtcgcggcag gaagcaaaat cggctcgagc gggtcgctgc cggacgggga
    13621 agaggggctt tacctgcaaa tacgttatca aggtcaggta ttgaaccctt cgagctggat
    13681 acgttgatcg gattgccgaa acggtaggct ttatgcaaat cgtgccgata tggtgcgctg
    13741 ttccgtgaag tttgccgtac ctgtattttc cggtttttgg aaatcaagag agaaagttaa
    13801 agaagaatgt cgaaacctgt ttttaagaaa atcgcacttt atactttggg tgcaatcagc
    13861 ggcgtggccg tcagtctggc ggtgcagggt tttgccgccg agaaggacag gcgggataac
    13921 gaagtcctgc cggtgcaatc catccgcaca atggcggagg tttacggtca aatcaaggca
    13981 aactactatc aggacaaacc cgatgccgat ttgtttgaag gtgcgatgaa gggtatggtg
    14041 gccggtttgg atccgcattc cgaatatatg gataaaaaag gttatgccga gataaaggag
    14101 tccaccagcg gcgaatttgg cggcttgggg atggaaatcg ggcaggaaga cggatttgtc
    14161 aaagtggttt cgccgattga ggacacgcct gcggaacggg cgggggtgaa aagcggcgat
    14221 ttcattgtga aaatcgataa tgtttcgaca cgcggcatga cggtcagcga agcggtgaag
    14281 aaaatgcggg gcaagccggg tacgaagatt actttgacgc tgtcgcgcaa aaatgccgac
    14341 aagccgatag tcgtcaacct gacccgtgcc attattaaag tgaaaagcgt ccgccatcac
    14401 ctgatcgaac ccgattacgg ctatatccgc gtgtcgcagt tccaagagcg gacggtcgaa
    14461 agcgtcaata ccgccgcaaa agagctgcta aaggaaaata aaggaaaacc gctcaagggg
    14521 ctggtgttgg atttgcgcga cgaccccggc gggcttttga ctggcgcggt cggcgtgtcg
    14581 gcggcatttc tgccgtctga agcagtcgtc gtcagcacca agggacgcga cggcaaagac
    14641 cgcatggtac tgaaagccat tcctgaagat tatgtgtacg ggatgggcgg cgattcgttg
    14701 gcgggcattc ctgccgagtt gaaaaccata ccgatgacgg tattggtcaa ttccggttcg
    14761 gcttccgcgt cggagattgt cgcaggtgca ttgcaggatc ataaacgcgc ggtcatcgtc
    14821 ggtacgcaga gcttcggcaa aggttcggtt cagactttga ttcctttgtc caacggcagc
    14881 gcggtcaagc tgacaacggc actgtattat acgccgaacg accgttctat tcaggcgcag
    14941 gggattgttc ccgatgtcga agtaaaagat aaggaacgca tttttgaaag ccgcgaggcg
    15001 gatttggtcg gacacatcgg caatcctttg ggcggcgagg atgtgaacag tgaaaccctt
    15061 gccgtgccgc ttgaaaaaga tgcggataag cccgctgtaa aagaaaaagg taaaaagaaa
    15121 aaggacgagg atttgtcttc aaggcggatt cccaaccctg ccaaagacga ccagttgcgg
    15181 aaagctttgg atttagtcaa gtcgcccgag cagtggcaga agtctttggg gctggcggcg
    15241 aaaaagccgg tttcaaataa agataagaaa gataaaaaag ataagaagta ggttttgacg
    15301 atgccgtctg aaaggatttc agacggcata ttatgtttga aaggacaggt tatgagcgat
    15361 tttttaccgg aatttgaacc gtgcgg
//
LOCUS       DAWGJV010000035        15218 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWGJV010000035 DAWGJV010000000
VERSION     DAWGJV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.035"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..619
                     /locus_tag="WHQ94_000799"
     CDS             <1..619
                     /locus_tag="WHQ94_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ2128115.1"
                     /translation="NGMTDNMLSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVK
                     PAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADF
                     GGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLL
                     VGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(826..1308)
                     /locus_tag="WHQ94_000800"
     CDS             complement(826..1308)
                     /locus_tag="WHQ94_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ2128116.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(1461..1622)
                     /locus_tag="WHQ94_000801"
     CDS             complement(1461..1622)
                     /locus_tag="WHQ94_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128117.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(1696..2157)
                     /gene="recX"
                     /locus_tag="WHQ94_000802"
     CDS             complement(1696..2157)
                     /gene="recX"
                     /locus_tag="WHQ94_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ2128118.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(2225..2932)
                     /locus_tag="WHQ94_000803"
     CDS             complement(2225..2932)
                     /locus_tag="WHQ94_000803"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ2128119.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRNHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            3339..4604
                     /locus_tag="WHQ94_000804"
     CDS             3339..4604
                     /locus_tag="WHQ94_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ2128120.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(4662..5468)
                     /locus_tag="WHQ94_000805"
     CDS             complement(4662..5468)
                     /locus_tag="WHQ94_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ2128121.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPIKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            5813..6022
                     /locus_tag="WHQ94_000806"
     CDS             5813..6022
                     /locus_tag="WHQ94_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ2128122.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            6111..7325
                     /locus_tag="WHQ94_000807"
     CDS             6111..7325
                     /locus_tag="WHQ94_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ2128123.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(7412..9991)
                     /gene="clpB"
                     /locus_tag="WHQ94_000808"
     CDS             complement(7412..9991)
                     /gene="clpB"
                     /locus_tag="WHQ94_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ2128124.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            10252..11262
                     /gene="trpS"
                     /locus_tag="WHQ94_000809"
     CDS             10252..11262
                     /gene="trpS"
                     /locus_tag="WHQ94_000809"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ2128125.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            11343..11888
                     /locus_tag="WHQ94_000810"
     CDS             11343..11888
                     /locus_tag="WHQ94_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128126.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENMAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNVVWLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            12207..12551
                     /locus_tag="WHQ94_000811"
     CDS             12207..12551
                     /locus_tag="WHQ94_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128127.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAAKDTLNKAADAT
                     QEAADKMKDAAK"
     gene            complement(12760..14268)
                     /gene="ppx"
                     /locus_tag="WHQ94_000812"
     CDS             complement(12760..14268)
                     /gene="ppx"
                     /locus_tag="WHQ94_000812"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ2128128.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDSMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(14384..15116)
                     /locus_tag="WHQ94_000813"
                     /pseudo
     CDS             complement(14384..15116)
                     /locus_tag="WHQ94_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3433 a         3800 c         4188 g         3797 t
ORIGIN      
        1 gaacggcatg accgacaata tgttgagcat cggtcagatt gttaaagtca aaccggcagg
       61 atatgccgca ccgaaaacct cagccgtaga aagcaggccc gccgtaccgg ctgccgtgca
      121 aacccctgtg aaacccgccg cgcaaccgcc tgtgcagtcc gcgccgcaac ctgccgcgcc
      181 cgctgcggaa aataaagcgg ttcccgcgcc cgccccgcaa tctcctgccg cttcgccttc
      241 cggaacgcgt tcggtcggcg gcattgtttg gcagcgtccg acgcaaggta aagtggttgc
      301 cgatttcggc ggcaacaaca agggtgtcga tattgcagga aatgcgggac aacccgtttt
      361 ggcggcggct gacggcaaag tggtttatgc cggttccggt ttgaggggat acggaaactt
      421 ggtcatcatc cagcataatt cttccttcct gaccgcatac gggcacaacc aaaaattact
      481 ggtcggcgaa ggtcagcagg tcaaacgcgg tcagcaggtt gccttgatgg gcaataccga
      541 cgcttctaga acgcagcttc atttcgaggt gcggcaaaac ggcaaaccag ttaatccgaa
      601 cagctatatc gcgttctgac tttctgcctg aaatcaagtt ggaaatgccg tctgaaaggt
      661 tttcagacgg catttttgtt tttggcggct gtgttttcaa atcattcgga agggacgggg
      721 catataaatt tgtttgactg tgtttgggta tttgcttgat ttcatccgtt tgcagtagaa
      781 acggatggcg cgatgtgcct gtctttcaga cggcatagtc cgccgtcagc agccgcagga
      841 catgtcttca gaggcttgca ggctgatgtc tctgcgtttt ttggcttttt cgtagcggca
      901 gcgttggcgg tcggtcagga tttccagcgg agggacaggg acgggtttgc cgtctttgac
      961 cgcaaccatc gtgaagtagc agctgttggt gtggcggatt tcgccggtgc ggatgttttg
     1021 tgcttcgaca cggatgccga tttccatcga ggtgcgtccc gtgtagttga cagcggcgta
     1081 aaaggtaacc aaatcgccga tgtggatggg ttctttaaac aggactttgt caaccgacag
     1141 ggtaacgcaa taattgccgc tgtaacggct ggcgcaggaa taggcgactt ggtcgagcag
     1201 gagcaggagt tcgccgccgt gtacgttgcc gctgaaattg gcggtgtccg gcatcatcag
     1261 ttcggacata atgagttcgt gggaaggcag ttggcgttgt tgtgtcatgg tgtacctgtt
     1321 ttaagaaagg gtgttttgaa atgggtgcgg ggaaatttta gggaatgggg aagtggaatt
     1381 caagctaaaa aaagctatga tgggatagtc tgccgaaatg gaccgccgat gccgtctgaa
     1441 gccttcagac ggcattttgt ttaggaaagc caaaaacgga agaccaggaa aaacacgagt
     1501 gccaatacgg cgacccaaac gacgagcatg aatccggcaa ctgcccgtcc catacggtaa
     1561 accgggtttt tgcgttcgtc ttcgatgggg gaggtgtggt gtttttcggg ttgtccggac
     1621 attttgttct caatcggaaa agttggaaac gggcgtaagg ttatgttgcc gtgcaaaaag
     1681 attcaagaag gaaggtcagc agccgtcttc ccaattttcg tcccaagcgt gtttcaatgc
     1741 cgtctgaacg gtatcggcat cgaatccgcg ataggcgagg aaacgcgcct gtttttgttt
     1801 ttctttgagg tcggcggcgg ggtgtttgaa ttttttacgc aacacggcta tggcggcctg
     1861 tttttcactt gagcggtcgg gaagcaggtt gcggctggtt tcttcatcta tgccctgttg
     1921 cgccaaagcc tgtttcagcc tcaatgaacc gtgtttgcgg cttttgctgc ggatataggc
     1981 ttcggcatag cgcaaatccg actgccagtt gcgttcggca aattcgttta acacgttttc
     2041 caactcttct tcgctttcgg cgtgcggtgc aagtttgcgt ttcagaccga tgcggctgac
     2101 ttcttggcgc gagaggatgt ccatcgcgcg ggcgcgtagg gatttttgcg gtttcatggc
     2161 ggcggtttcg gcgtggggca ggtttcagac ggcatagcgg cgcaaaccgg agccgtccga
     2221 atgcctactc ttctttgttt ttctgaggtt gcaggttttc gtaaatttcg ggcagcgagc
     2281 cgataatcca gtcggggcgg gtcgcatcgt cttgcgagag cagcgtcata tcgccgtaac
     2341 cgaaggtaac gccgacgctc aggcagccgg cggctttggc ggcgatgatg tcgttgcgcg
     2401 agtcgccgac cataaccatg tttgcaacat cgatacccaa aacttcggcg gcgtgccgca
     2461 gcggcagggg gctgggtttt ttctcgggca ggctgtcgcc gccgagtatc aggctgaagt
     2521 agtcggcaag tccgagctgt ttcaacagtt cgacggcaag gatttcgttt ttgttggtaa
     2581 tgactgccag cgggatgccc aaagatttga gcaatgccag cccggcttcg gtttcgggat
     2641 aggggcgggt gaagacgctc aaatggttgc ggtagtattt catataggat acgaaacctt
     2701 tttcccacag ttcggaatcg gcttcgcggt cgcggtcgtt ggtgaggacg cggtgaacca
     2761 gtttgccgat gccgtcgccc acatagcttt cgaccacttt ggcgggcagc ggtttcatac
     2821 cgagttgttc caacatcgct tctgcggcgg cggcaaggtc ggggacggaa tcgcacagtg
     2881 tgccgtccaa atcgaaggcg acggcttgga cgtgttcgat ggctgcattc ataatctgat
     2941 ttcctgtcgg gttgaaaggg tttattttaa catcgtttgc acagggcttc taatgtatgg
     3001 attgttaaca atcgcaacag gttttgcagg cggtgttttc ggatttcggg gcggaatcgg
     3061 gcaagggttt gaaatcccag cgatgttcca attaaatttt taaaaaataa aaggatgcct
     3121 atatctttat ttaatttgac atttattttt cggacgcgcg cgctgctttt tcaggcggag
     3181 tccgggctga ttccgtgcgg cttgaaaaat aaaatcatat atagattaat caaatcgggc
     3241 ttatgccgac tacacgctat tacattttgt cgggaaattg gttatagtaa tgagcgaaat
     3301 gcacgcccct gaaaatcttt ttgtgaaaag gaagcaaaat gtctgaagcc cttgcaaaag
     3361 aaaccctcaa tccgtttgaa atcgcgcgca aacaggtcaa aaccgcctgc gaccggctga
     3421 aaaccgatcc ggccgtttat gaaatcctga agagtccgac ccgcgtgttg gaagtcaatt
     3481 tccccgtcaa actcgacgac gacacggtca aaaccttcac gggctaccgt tcgcaacaca
     3541 acaacgccgt cggcccctat aaaggcggcg tgcgcttcca tcccggagtg aatctggacg
     3601 aagtcaaagc cctgtcgatt tggatgacca tcaaatgctg cgtagcgggc attccttacg
     3661 gcggcggcaa aggcggcgtt accttggatc cgcgcgatta ttccgaggcg gaactggaac
     3721 gcatcgcccg cgcttattcc gaagccattt ctccgctgat cggcgaaaaa atcgatattc
     3781 ccgcccccga tgtgaacacc aacggcaaaa tcatgtcttg gatggttgat gcctatgaaa
     3841 acgtggtgaa acattctgca ccgggcgtat ttaccggtaa accggttgag ttcggcggct
     3901 ctttggcgcg taccgaggct accggttacg gcgtgaacct cgccgccgtc caagccttgg
     3961 aaaaactggg caaggacgtg aaaggcgcga cttacgccat ccaaggcttc ggtaatgtcg
     4021 gttaccacac aggttattac gcgcatcaat ccggcgcgaa agttgttgcg gtttctactg
     4081 ttgacgttgc catttacaac gaaaacggtt tggatatgga agccctgttc aaagagttcc
     4141 aagaaaaagg cttcatcacc aacgaagccg gttacggcaa ggaaattacc aacgccgaac
     4201 ttttggcttt ggatgtggac gtactcgccc cttgcgcgct ggaaaatcaa ctgacttctg
     4261 aaaacgccgg taaagtccgc gcgaaaatcg tggtcgaagg cgcgaacggc ccgactacgc
     4321 ccgaagccga cgttatcctg cgtcaaaacg gcgtattggt cgtgcctgat attctggcga
     4381 actgcggcgg cgtggtcgtt tcctatttcg agtgggtgca aaacctgcaa ggctattatt
     4441 gggagtttga cgaagttcaa gagaaagaaa ccgtcgtcct gcgccgagcg ttccgcgata
     4501 tttggaactt ggctcaagaa tacgatgtcg atttgcgaac cgcgtcttat atgatgagta
     4561 tccgccgtgt tgaaaaagcg atgaaactgc gcggttggta ttgatgtttg aacaaaaaat
     4621 gccgtctgaa gccttcagac ggcatttttg tgcgggcggg attaatggtg cgaatggccg
     4681 atttggaagg ttaaagtcga gtagttcgcc tgtttttggc acacgctttg atcggggaag
     4741 tcggctttgt gttcgacatt ggctttccag aagccttggc gcaaggggat gatgtccact
     4801 tcgcctttgt cgtctgtgct gtcggagaaa gcctgtgctt cggttttgtg cgttttgctg
     4861 cggtcgctgg tgtcgaagcc gtcaaaggtg gcggtaacgg tggcattggg cagcggttcg
     4921 ccacggaaca gaacgcggac tttgaagcgt tcgcctacgt gaatgttggc gggattgtcc
     4981 agcgggacga tttccaagtt ttgtccgacc ggtttggtga tgatggcggt gtccgcgctt
     5041 tcgtgtccga cgttgacgat gtttttgccg aacatacggg tttgttcgca atagcttgcg
     5101 tcaggcattt ctttgatgcc cgcctgtttc cagcctgctt tgttttttga ccagaaagta
     5161 ggctgatatt cggcgatgac gaggtaactg ccgtccttaa tgggacggtt gcttcggtac
     5221 tggtagttgt atgtgccgcg ttgaatcatg ttttccttgc ctttttcggt aaccagctgc
     5281 atcggtttgc tgaagatgtg caggcggtct ttggcgatgg gttcgagttc gggaaattcg
     5341 ccgtagccca agtcggcttt aaggtattcg ccgccgtgcg tgtgggcggt ttcgacccag
     5401 acgcggtggg cgtgggcgga tgtggcaaat agggcggaaa cggcgagcag tgtcaatgtt
     5461 ttcttcatgg tgcagccttt gtctcgtgtg ggttaaagat tttgttatgt tataacaaaa
     5521 taggtatcgt gtgaagacgg atttgctgtc tgaaaactgt ctttttgtat agtggattaa
     5581 caaaaaccag tacggcgttg gctcgcctta gctcaaagag aacgactctc taaggtgctg
     5641 aagcaccaag tgaatcggtt ccgtgctatt tgtactgtct tcggcttcgc cgccttgtcc
     5701 tgatttttgt taatccgcta tatcgatttg aatttttcag aaaatgaagc ggacgtttgg
     5761 gcggaggcaa cttgtttgat aagatagcaa tattttaaaa cggagaaaga tcatgcctta
     5821 tgtcaatatt aaagtaaccg gcggcaagga agcaccgact gccgcgcaaa aagcggaact
     5881 gatcggcggc gtaaccgaat tgctggcacg cgtgctgggc aaaaatcccg aaacaacggt
     5941 tgtcgtgatt gacgaagtgg ataccgataa ctggggaata ggcggcaaaa gcgtcagcga
     6001 acggcgcaaa gagggcaggt aaaaagcctg aaaatctcgg tttgatgctt taaattccgc
     6061 gtgaaaaaga gtacattccc acccattgcc caaaatttac ggaacacatc atggataaat
     6121 ttcccaagtc tgcaaagctc gatcacgtct gttacgacat acgcgggccg gttcacaaaa
     6181 aagcccttca gttggaagag gagggcaata aaatccttaa actcaatatc ggcaaccctg
     6241 cgccgttcgg ctttgaagcc cctgatgaaa tcttggtcga tgtcatccgc aacctgccga
     6301 cttcgcaagg ctattgcgat tctaaagggc tgtattccgc ccgcaaagcc attgttcact
     6361 actatcagac caaaggtttg cgcgatatta cggttgatga tgtctatatc ggcaacggcg
     6421 tgtccgagct gattacgatg tctatgcagg cattgctcaa cgacggcgac gaaatcctga
     6481 ttcccgcgcc cgactaccct ttgtggacgg cggcggcaac gcttgcgggc ggtacggtac
     6541 gccattatct gtgcgacgaa gaaaacggct ggttccccaa ccttgccgat atggaagcca
     6601 aaatcacgcc caaaaccaaa gccatcgtcg tcatcaatcc caataatccg acaggcgcgg
     6661 tgtacagcag ggaaatcctg ttggaaatcg ccgaactggc gcgcaagcac ggtttgatta
     6721 ttttcgccga cgagatttac gataaaatcc tttatgacgg cgcggttcac caccacatcg
     6781 ccgcgcttgc ccccgacctt ttgacggtaa cgttcaacgg tttgtccaaa tcctaccgcg
     6841 tcgccggctt ccgccaaggc tggatggtgc tcaacgggcc gaaacatcac gcaaaaggtt
     6901 acatcgaggg tttggatatg ctctcgtcca tgcgcttgtg tgccaatacg ccgatgcagc
     6961 acgcgattca gacggcattg ggcggctatc agagcattaa cgaatttatc ctgccgggcg
     7021 ggcggctttt ggaacagcgc aacagggcgt gggaactggt cagccaaatc cccggcgtgt
     7081 cctgcgtcaa accgatgggc gcgatgtata tgttcccgaa aatcgatacc gaaatgtacc
     7141 gcatccgcga tgacatgaaa ttcgtttacg atttgctggt gcgcgaaaaa gtcttgctgg
     7201 tgcagggaac ggggtttaat tggattaaac ccgaccattt ccgcattgtt acgctgcctt
     7261 acgtccatca gattgaagag gcgatgggca ggctggcaag attcctgcaa acctaccgcc
     7321 aataagggga cggtttgtct gccgaggata aaaaatgccg tctgaaacgg agattcccgt
     7381 ttcagacggc atttccaaca gcaggaacga atcaggcaaa tttcagtctg tcgccgtcgg
     7441 cttccaccct gatttcgctt tcgggcgcat agtttccggc aagcagggct tttgccagcg
     7501 ggttttcgat ttccgactgg atggcgcgtt tgagcggacg tgcgccgtaa atcgggtcga
     7561 aaccggcttt ggcgatgatg tccagtgcgg catcggaaac agtcaggcgc aggttttgtt
     7621 tttccaaacg tttttccaaa cctttgagct ggattttcgc aatgctgcgg atgtttgcct
     7681 gatccaaacc gtggaacacg accacttcgt caatacggtt gatcatttca ggacggaaat
     7741 gttctttcac atcctccatc acaacttctt tcaccgcttc gtagtcctgc gtgcccattt
     7801 gttggatgtg ctggctgccg atgttggaag tcatgacgat aacagtgttt ttgaagtcca
     7861 cggtgcgacc ttgcccgtcg gtcaggcggc cgtcatccaa tacttgcaac aggatattga
     7921 acacgtcggg atgggctttt tccacttcgt ccaacagaat cacgctgtac ggtttgcggc
     7981 ggacttgttc ggtcaggtag ccgccctctt cgtagccgac atagcccgga ggcgcgccga
     8041 ttaagcgggc aacggcgtgt ttttccatat attcggacat atcgatacga atcagatgat
     8101 cttcactgtc aaacaggaag cctgccaatg ccttacacaa ttcggtctta cccacgccgg
     8161 tcgggcctaa gaacaggaag ctgccgtaag gtttgttcgg atcggcaaga ccggaacggc
     8221 tgcggcggat agcgtcggac acggcacgca cggcttcatc ttgaccgacc acgcggcggt
     8281 gcaatacttc ttccatcttc agcagtttgt cgcgttcgcc ttccatcatt ttggacacgg
     8341 gaataccggt catacgggaa acaacttctg cgatttcctc cgcgccgaca ttattacgca
     8401 agagtttgtt tgccggtttt gtgctgtccg tatctgcccg ttcggcggct gcacgctgtt
     8461 tttccaaatg ctccaaatct tcatacatca attttgaagc cagtgccaaa tcgccttgcc
     8521 gttttgcctg ttcgatttta attttgactt cgtcaatttg tttcttaata ttagcagcac
     8581 cgtctgaaat tgctttttcg gctttccaga tttcgtctaa atcggcgtat tctttttgca
     8641 gaccgttgat ttcctcgtct atcagttcca aacgtttttt gctggcatcg tctttttctt
     8701 tttcaacgtg tgccttttcc atccgaagct gaatcagacg gcggtcgatt ttgtccattg
     8761 cttccggctt ggtttctttt tccatcttga cacggctggc ggcttcatca atcaaatcaa
     8821 tcgctttgtc gggcaggaag cggtcggtga tgtagcggtc gctcaactcc gctgcggcaa
     8881 cgatagcagg gtcggtaata tcgataccgt ggtggatttc ataacgctcc tgcaaaccgc
     8941 gcaagatggc gatggtgtct tccacgctgg gctcgccgac caatactttt tggaagcggc
     9001 gttcgagtgc cgcgtctttt tcgatgtatt ggcggtattc gtccaaggtg gtcgcgccga
     9061 tacagtgcag ttcgccccgc gccaaggccg gtttcagcat attgcccgcg tccatcgcgc
     9121 cgtcggtttt gcccgcgccg accaaagtat ggatttcatc aatgaaaatc agagtgttgc
     9181 cgtcgtcttt cgccaaatcg ttcaacacgc ctttcaagcg ttcttcaaat tcgccacggt
     9241 atttcgcgcc ggcaatcaat gccgccaagt ccagaaccaa aaggcgtttg ttgcgcaggg
     9301 attcgggtac ttcgccgttg acgatgcgtt gcgccaagcc ttcgacaatg gcagttttac
     9361 ccacgcccgg ttcgccaatc agcacggggt tgtttttggt gcggcgttgc aatacctgaa
     9421 ttgcacggcg gatttcgtcg tcacgaccga taacggggtc gagcttaccg tcgcgggcgc
     9481 gttgggtcaa atcgagcgta tattttttca gcgcatcgcg ttggtcttcg gcattggcat
     9541 cgttcacgtt ttgtcctcct cgtaccgcat cgattgcggc attaatgttt tgttcggtcg
     9601 cgccggcttc tttcaaaatt ttgccggttg catcgttctg ctgcaccaag gcgagcagga
     9661 aaagttcgct ggcgatatag gcatcgccgc gtttggttgc tgctttgtcc atcaggttca
     9721 acaccgcctg caattcgcgg ctgggcataa tgtcgccgcc ctgtccggat actttcggca
     9781 ggctgtttaa atgctgctgc aaacgctgtt tcacctgcgg cacgttcacg cccgcatgag
     9841 ccaagagcgc ggcggctccg ctgttttggt cgtcaagcag ggcttttaac acaaagcccg
     9901 cttccagata gctgccgtcc gcagccaacg ccaaactctg agcttctgca agggcttgtt
     9961 ggaatttggc ggttaatttg tcgtatcgca tttttgtttc cttttcaaaa tgtccgctgt
    10021 cgaagcctat atgtgcataa ttgtggataa ctcaagttct gttttctgtt tttctatatt
    10081 taattcgata tatcattgaa tttaaagtat ataaaaatgt ataataatgt gtataactat
    10141 atcttcttaa tatggaaaag tctgttgtcg gctggatgta ggtggcaaat cgggtataat
    10201 cggcgcatct ttttcccttt cagacggcat tgatgccgca aggacatttt tatgagcaaa
    10261 aaacgagttc tgaccggcgt aaccaccacc ggcatcccgc atctgggcaa ctacgtcggc
    10321 gccatccgcc ccgccgtccg cgcggcgcaa aaccctgata ccgaatcctt cctcttcctc
    10381 gccgattacc acggtatcat caaatgccac gagccggaga tgattcacca atccacccaa
    10441 gccgttgccg ccacttggct tgcctgcgga ctcgaccccg agcgcaccac cttctaccgc
    10501 caaagcgaca ttcccgaagt gatggaatta aactggattc tgacctgcat caccgccaag
    10561 ggtttgatga accgcgccca cgcctacaaa gccgctgtgc aggcaaatgc agaaaacaat
    10621 caggaagatc ccgatttcgg cgtggaaatg ggtttgttca gttatccgat tctgatgact
    10681 gccgacatcc tcatgttcaa cgccaacgaa gtgcccgtcg ggcgcgacca aatccaacac
    10741 gtcgaaatgg cgcgcgacat cgccggccgc ttcaaccacc gctttaaaga aatctttacc
    10801 ctgcccgaag tgaaaatcga cgaaaacgtc gaactcttgg tcggtttgga cggacgcaaa
    10861 atgtccaaat cctacggcaa caccattccg ctttgggaaa acgacaaaaa aacccaaaaa
    10921 tcggtcaaca aaatcatcac caatatgaaa gagccgggcg agccgaaaca gcccgacgaa
    10981 agcccattgt ttgaaatcta caaagccttc tccacgccgt ctgaaacggt ggaatttacg
    11041 aaaatgcttg ccgacggctt ggcttggggc gaggcaaaaa aacttttggc ggcgaaaatc
    11101 aacgccgaac tcgccgaacc gcgcgaacgc tacaacgagc tgaccgccga cccttcgcaa
    11161 atcgaagaga ttttacaggc aggcgcgcaa aaagcgcgta aagaagcacg cgaattgttg
    11221 gacaaagtac gcgatgcggt cggcatccgc ccgttgaaat aaacccaatg ccgtctgaac
    11281 atccattgtc cgtgtttcag acggcatttc agacggcatt ttgaaaccat caggagtgtg
    11341 gaatgcttaa aacatctttt gccgtgttgg gcggctgcct gctgcttgcc gcctgcggca
    11401 aatccgaaaa tatggcggaa cagccgcaaa acgcggtaca aagcgcgccg aaaccggttt
    11461 ttaaagtcaa atatatcgac aatacggcga ttgccggttt ggctttggga caaagcagcg
    11521 aaggcaaaac caacgacggc aaaaaacaaa tcagttatcc gattaaaggc ttgccggaac
    11581 aaaacgtcgt ctggctgacc ggaaagcata ccaacgactt ggaagccgtc gtcggcaaat
    11641 gtatggaaac cgacggaaag ggcgcacctt cgggctgggc ggcaaacggc gtgtgccata
    11701 ccttgtttgc caaactggtg ggcaatatcg ccgaagacgg cggcaaactg acggattacc
    11761 tgatttcgca ttccgccctg caaccctatc aggcaggcaa aagcggctat gccgccgtgc
    11821 agaacggacg ctatgtgctg gaaatcgaca gcgagggggc gttttatttc cgccgccgcc
    11881 attattgagg tattcggaca tcccggaata tatttgggtt tttcaaacct tgcgaaaaaa
    11941 gtccgcaccg ccgggaaact caaaaggagg gggatatgtg ttacaatttt ccgaactgtt
    12001 ttcataaaat agttttcgga cgtgtttcaa tatggcattg atgccgcctt attgttcgag
    12061 aaaaaaacct ttatatttaa atataatggg ttttaactaa acgggaaacc gttttctctc
    12121 cggccgatgg gcaaaatcag ccgattgatg gaacacggtc cgatttttaa gcaaaacctt
    12181 aatcaacaac tcaagaagga taagacatga aaaaattatt gattgccgca atgatggcgg
    12241 ctgccttggc agcttgttcg caagaagcca aacaggaggt taaggaagcg gttcaagccg
    12301 ttgagtccga tgttaaagac actgcggctt ctgccgccga gtctgccgct tctgccgtcg
    12361 aagaagcgaa agaccaagtc aaagatgctg cggctgatgc aaaggcaagt gccgaggaag
    12421 ctgtaactga agccaaagaa gctgtaactg aagccaaaga agctgtaact gaagcagcta
    12481 aagatacttt gaacaaagct gccgacgcga ctcaggaagc ggcagacaaa atgaaagatg
    12541 ccgccaaata atttgttgcc ttggcaaaaa tgatgggatg ccgcctgccg gcaacccaaa
    12601 cgaaaccgcc tgaagatttt caggcggttt ttgagttgta tgccccttgt tttttacgcc
    12661 tgcatgaccg ttccgagcag atgtgccgcg catatcggga tgttgcggac aggcaaatgc
    12721 cgtctggaca gggtttggac ggcatttgtt ccgtaaggtt cagacggcct cgactttaaa
    12781 cggcatattg attttttgcc attggacgct ttcatattcc aatgcgtcgg caatcagggg
    12841 atggcgttcc agccattccc tgtcaatacg caggatgaag ccgcagcttt ccgtatccgt
    12901 gcgcaactgc atatttttcg ggaaagacag gtcttggcgc gaacggcaga acagtgcggc
    12961 aaggcgcagg gacaaaacgg cataccacaa catttcgttg gtgccgatga tgccgctcat
    13021 ttttttcata tcgccgcgat gaccgatgac cagttgggca agtatggtct gttctttgcg
    13081 tgagaaaccc ggcatatcgg cgttttcgag gatgtaggcg gaatgcttgt gatagccggt
    13141 gtgggcgatg tccaaaccga tttcgtgcag cgcggcggcg cgtccgagat actgttgcca
    13201 taaggcaagc tcttgaactg taacgttttt agcgtggcag aggctgtcca taaaggtttg
    13261 cgcggtctcg gcggtgcgtt tcgcctgatt gaggctgacg tggtagcggt gttggaactc
    13321 ggcaaccgtt tgtccgcgca tatcttcgtt taaaccgcgc ccgatcaaat cgtaaaacac
    13381 gccgtcgcgc agggcggctt cggttacggt catactgtcg agtttcattt cctcaaacgc
    13441 cgccatcatc acggcaagtc cgccggcaaa aacttcgatg cgttccggtt tcaggttttc
    13501 aaatttggct tttttgaccg aaccggcttc gatgatgcgt tcggcaaggg cgcgcatacc
    13561 tttgtaggta atgtccgcct cttggggcat ttcggcggca agcacgtcgc ggatggattt
    13621 tgccgaaccc gatgtgccga cggcgaaatc ccaaccttcg cgcctcatat ttttgctgat
    13681 gcgctggatt tcgttgcggg cggcggaaat ggcagattgg aagtctttgg cggtgatttt
    13741 gttttggaag aagcgcaggc tgtaggttac gcagcccaag ggcaggcttt cggtaatgtc
    13801 gggattcagc gtcgagccga tgacaaattc tgtcgaaccg ccgccgatgt cgataaccag
    13861 cattttgccg ccgcccgggg ggagggtgtg gatcacgccg gtataaatca gccgcgcctc
    13921 ttcgcgcccg gcgatgattt cgatggggaa acccagtgcc gcttcggcct tgggaaggaa
    13981 atctgcgatg tttttggcga cgcggaatgt attggttgcc acggcgcgta cctgttcagg
    14041 gcggaagccg cgcaggcgtt cgccgaattt tgccagacag tccaaagcct gttcttggga
    14101 agcggcactc aggtttttct gttcgtccag accggcggcg aagcgcacca tctgtttgaa
    14161 cgaatcgatg acttttaatt gtccgttgtt gttttcgcaa atctggaggc ggaaactgtt
    14221 ggaacccaaa tcgacggagg cgaggatgtt tgcgggggtg gtggtcatgg cggatatcgg
    14281 tgggggaaaa acgcaatgtt actctgacgg cgcaggcgtt gacaataaat gatgcggcgg
    14341 tttttgattc tgcccacgga tgttgccgac ggcatttttg cgcttatttg aaatcctttt
    14401 ccacgctcat gaaaatctgc gtgtttttgc gtgtgtaaaa acttttcata ttgctgtcga
    14461 ttttcagata gcggaaattg agttgcggcg taaagccctt ccaagagatt ttgtcgtgcc
    14521 acaacgacag gtttgcctga tattcgtggt ctttgcgcgg gaagcggtac acaatggtcc
    14581 cgggtgcgtc aaacatcctg cgggtatagc gcaggtttgc ccgcagaccc aagcccgccg
    14641 tcgaacgttt tgactgcgcc gacacgcaaa cccttgcgga cggaagcctg ttccgcctct
    14701 ttcgtcatgt tgtgcgacca gtccgcaccg ccgtaaagca gccagtcttt cggcgcggaa
    14761 tacatcagcg tcgcgcccgc cagcggcata tggctgtcgt atcgggcggc ggtgcggtct
    14821 tcctgataac gcttccacat attgcccacg ttcagcgtca gccgccagcg ttcgctcaag
    14881 cgtcgggaga aatcggcatt gaagccgccg acgaaattgt atcggctgcc gcctaagagg
    14941 ttttgcccga taaacggcac gatgccgaac gagcgcgtta ccgaacgatt tttatagccg
    15001 agcgacaggc gcaggcactg ttcgctgaaa tctttgttat cccaataatg cacgccgccg
    15061 ccgctgatgc cgccgtagag gaaatgatgc ccgcccgcat tgatttcgcg cgacacgcct
    15121 aagccctagc gcaaaccgtg tgccttttgc ggcaggctgt cggcggtttt cgtccagctt
    15181 ctgcccgcaa attcaatcgt ttttttggac gaagcgtt
//
LOCUS       DAWGJV010000036        15088 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWGJV010000036 DAWGJV010000000
VERSION     DAWGJV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.036"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(79..471)
                     /gene="rpsI"
                     /locus_tag="WHQ94_000814"
     CDS             complement(79..471)
                     /gene="rpsI"
                     /locus_tag="WHQ94_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ2128129.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(481..912)
                     /gene="rplM"
                     /locus_tag="WHQ94_000815"
     CDS             complement(481..912)
                     /gene="rplM"
                     /locus_tag="WHQ94_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ2128130.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1090..1269)
                     /gene="ssrS"
                     /locus_tag="WHQ94_000816"
     ncRNA           complement(1090..1269)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WHQ94_000816"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1275..1580)
                     /locus_tag="WHQ94_000817"
     CDS             complement(1275..1580)
                     /locus_tag="WHQ94_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ2128131.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1591..1920)
                     /locus_tag="WHQ94_000818"
     CDS             complement(1591..1920)
                     /locus_tag="WHQ94_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128132.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(1975..2964)
                     /locus_tag="WHQ94_000819"
     CDS             complement(1975..2964)
                     /locus_tag="WHQ94_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ2128133.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3087..5789)
                     /gene="ppc"
                     /locus_tag="WHQ94_000820"
     CDS             complement(3087..5789)
                     /gene="ppc"
                     /locus_tag="WHQ94_000820"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ2128134.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5832..6725
                     /locus_tag="WHQ94_000821"
     CDS             5832..6725
                     /locus_tag="WHQ94_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ2128135.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(7124..7348)
                     /locus_tag="WHQ94_000822"
     CDS             complement(7124..7348)
                     /locus_tag="WHQ94_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ2128136.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7349..8737)
                     /locus_tag="WHQ94_000823"
     CDS             complement(7349..8737)
                     /locus_tag="WHQ94_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ2128137.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLVFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8823..9644)
                     /gene="prmC"
                     /locus_tag="WHQ94_000824"
     CDS             complement(8823..9644)
                     /gene="prmC"
                     /locus_tag="WHQ94_000824"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ2128138.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(9634..10095)
                     /locus_tag="WHQ94_000825"
     CDS             complement(9634..10095)
                     /locus_tag="WHQ94_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128139.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(10160..11644)
                     /gene="tldD"
                     /locus_tag="WHQ94_000826"
     CDS             complement(10160..11644)
                     /gene="tldD"
                     /locus_tag="WHQ94_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ2128140.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRMNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDSAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASVPMPRMTNTFMENGGYEPDEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      11719..11822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11889..13112
                     /gene="cytX"
                     /locus_tag="WHQ94_000827"
     CDS             11889..13112
                     /gene="cytX"
                     /locus_tag="WHQ94_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ2128141.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            13109..14209
                     /locus_tag="WHQ94_000828"
     CDS             13109..14209
                     /locus_tag="WHQ94_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ2128142.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRNIRGHLKTPAMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAIHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKNAPERDEESGLAYIRRQD"
     gene            14231..14848
                     /gene="thiE"
                     /locus_tag="WHQ94_000829"
     CDS             14231..14848
                     /gene="thiE"
                     /locus_tag="WHQ94_000829"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ2128143.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3143 a         4298 c         4210 g         3437 t
ORIGIN      
        1 tctttagatt gccaatggga aaccgtctac tacaaataaa aaaccctgcg ataagcaggg
       61 ttttttgaat ttccaacatt aacgtttgga gaattgtttt gcacggcgtg ctttgcgcag
      121 acccggtttt ttacgttcga cttcgcgggc atcgcgggta acaaaaccag cttgagacaa
      181 ggcgggtttc aacgcggcat cgaagtcgat cagggcacgg gtaatgccgt ggcggattgc
      241 gccggactgg ccggtttcgc cgccgccaac aacattgact ttgatgtcga aagattcggc
      301 gttttcagtc agaaccaagg gttggcgaac aaccattcgg ctggtttcgc gtgcgaagaa
      361 ttcgtcaacg ggacggccgt ttacgatgat ttgacctgta cctttaatca ggaatacacg
      421 agccactgaa cttttgcggc ggcctgtgcc gtagtagtat ttaccgttca tgtcgcgtcc
      481 ttatttcagt tccaaaactt tgggttgttg cgcagcatgg gcgtgttccg cacccgcata
      541 cactttcagt tttttaatca tggcgtaacc caaaggacct ttgggcagca taccttttac
      601 agcttgttcc aaagcgcggc ccgggaattg ctcttgcatt tcgcggaaag tgcgttcgta
      661 gataccgcct gggaaaccgg aatggcggaa gtatttttta tcttcgaatt tggcaccggt
      721 tacacgcagt ttgtccgcat tgataacaat gatgtaatcg ccggtatcga cgtggggggt
      781 gtattcaggt ttgtgtttgc cacgcagacg gctggcgact tcggccgcaa cgcgacccaa
      841 gactttgtct tgggcatcga tgacgaacca ttcgcgcttc acctcgtggg gtttcgctga
      901 aaaggttttc atagtggaaa tccagataga tatagaaagt tgtaaatttt aaagacagga
      961 ttcgattttg tcaatcgcat taccgcgtta cggaaggatt ttccggattt cggcagactg
     1021 catactgctt tttcgggcgg ggcggcggcc aatgtgaaaa accgcatcgt tgcgatgcgg
     1081 ttttgaatgg gaatccccgc gagagccgtt tcggccgaat ccgcttgaac cttgctgaca
     1141 aggcggctgc ctcgggtagt ttcgggtgcg tccgcaaaag gacgctcgcg cccactactg
     1201 ctcccggcaa ccttaagcga acttattggt tcaaaggaat atatgccttc gcggacaccg
     1261 cagggaaaaa ggggttattc ctgcgccaag cgggatagtg ctttttggca ggcgttgtcc
     1321 atatcggcta ttttacgcgc aaaatcgccg attgccaaat cgccgccgtt cagggaggtt
     1381 ttcaacaggt cgtggacgac gttgagcgcg gccataatga cgattttttc gctgtccgcg
     1441 acgcgtccgc cttcgcggat ggcttcggct ttgccgttga gcattccgac tgcctgcaac
     1501 agtgtgtctt tttcttctgc cggcgtgttg acggtcagcc gggcgtgcat gacttcgatg
     1561 tggacttgtt cgatgttcat cctttaatcc ttattgctgc gtttcctgcc attgggggag
     1621 gcgcgctgcc agtgcgctga ttttttccct gctctgttcg agcaggctgc ggtatcgtgt
     1681 attttcttct gtcaggctgt caattttgtt ttgcaggtct tctttgagtt tgccgacttg
     1741 gacgagcagg gcttcgctga gttcgtcgac ggcggtttcg tgttcgagtt tttgccgctc
     1801 gtgcgcccgt ttgagttcgg cgacggtttc tttgaggcgg cggttttcgc tgacgagggt
     1861 ttcgaatttt tgtaccagcg tataaacgct gctttcgagt ttttcgatat tttgtttcat
     1921 aaccttacct gtccgtatgc cgtctgaagg cctcagacgg catctgtctg ttgtttattc
     1981 gaaacgcgcg ctgcgttcca tcagtctttc gacgacctgc tgaggggtca tttccttacg
     2041 gatgagttgc agcagggttt gggtaatcgg catatcgatt tggtacttac aggcagtatt
     2101 gaagacttct tctatcgtgc tgaccccttc ggaaacgtgt ccgatttcga ccagcacctg
     2161 atgcagttcc ttgccttctg ccaaacccaa gccgacgcgg cggttgcgtg agagtgcgcc
     2221 agtgcaggtc aagatgaggt cgccgatgcc tgccagcccc atcatggttt tgggctgtgc
     2281 gcccattgcg gaggcaaggc gggtaatttc agctaatccg cgcgtaacca gtgcggcacg
     2341 ggcgttaagc ccgtactcta ggccatcgga caatccggtg gcaatcgcca taacattttt
     2401 taccgcgccg ccaaccgcca cgccgataac gtcggtactg ccgtaaagcc tcatgacagt
     2461 cgtgttgagc tgcggtacga gttcttcaat ccactcttgg ttttcggagg cgaggacgac
     2521 ggcacagggc agttgtttgg cgagttcctg tgcaaaactc gggccggaga gtacgccgat
     2581 tttcttattg tcgggcaata cttctttcaa tacttgaaag gtcagcagcc ctgtatcctg
     2641 ctcgaatcct ttgcaggcgg cgaggacggg gaggtgtccc gcgccgtact gtttgagcag
     2701 ctctgcgctg cttctcaatc cggcaacgga ggttacgata aggacaagtc cgctgtcttt
     2761 gagcgcgtct gccaaatccg cacacacttc caaggtttcg ggaaaggaaa agccgggcag
     2821 tccgcgtttg ttttcacgcg cttcctgcat ttggcggact tggtctgcgt tgcgcgtcca
     2881 cagggatacg cggttgccgt gttgggaaaa gtgcagggcg agcgccgtac cccacgaacc
     2941 tgcgccgata acggtaattt tcattggtcg tctttcaaca tatcactgcc gttcacttta
     3001 aaacaatcgg tgtttctctg caagtgcggt cagggaaatg ccgtctgaaa ggtgttcaga
     3061 cggcattttg ccccgatgcg gcacaatcag cctgtattgc gcaaaccttg cgccacgccg
     3121 ttgatggtca ggtgaaccat cagcagcgcg tgcggattgt cgggttcttt acgcaggcgt
     3181 ttgagcatgg cgacctgcag gccgttgagc gcgttcaaat atggaatcct caaggcaagc
     3241 gaacgggcga ggctgcggtt gtcgcgtaaa agttcttcgg tttgcagcag gtcgagcagg
     3301 gctttgcggc tgcgttggta ttcttcctta atcatgccga agatgacggc ggctttttcc
     3361 ggcgattcgc tcaagccggc atagttttcc gcgagggtga tgtcggtttt cgccatcact
     3421 tgctccatat tggaaagcat ggcttggaag aacgggttgc tttgggcgtg ttcgcgcagg
     3481 gcggcgagcg tttcgggttt gtcttcgcac aaggtttcca ccgcgctgcc gaaaccgtac
     3541 caagcaggca gcatgaggcg gttttgcatc catgagaata cccacggaat agcgcgcaag
     3601 tcctgaatcc gcgccaaggt tttgcggctg gcaggacggc tgccgaggtt gagggtggcg
     3661 atttcctgaa tcgggctggt ttgcaggaag tagtcgatga agtcggggtg ggtaatcagt
     3721 tcgcggtagt atttgaacga tacgtccgac aatgcctgca tcagtttggc atcagggtct
     3781 tttttatccg gcaggatgct ggcttccaaa gtcgcggcaa ccaaggtttc caagttgcgt
     3841 tgggcattgc cgggatcggc gtatttggcg gtaatgactt cgccttgttc ggtaatgcgg
     3901 atttgtcccg ccacgctgcc cgccggttgg gcgagaatgg cttggtaaga agggccgccg
     3961 ccgcgtccga cgctgccgcc gcgaccgtgg aacaggcgca tgcggacatc gtattttttg
     4021 aagagttcga ccaagcccaa ttccgcctga tacagacacc atgagctggt aacatagccg
     4081 ccgtctttgt tggagtcgga gtagccgagc ataatttctt ggatgttgcc gcagctttcg
     4141 agcagggcat cgtaccaatc gaggcggaac atggtttcca tgaccggaca ggcgttttcg
     4201 agtgcttcga tggtttcaaa cagcggcacg atattgatgc ggctgcgcgg tttgccgttt
     4261 tccaccgcca gcaggccgct ttctttcagc agcaatgcca aggcgagcag gtcgctgggt
     4321 tgttcgcagt tggaaataat gctttgtgta acggcatctt cgccgaattc gtctttgatt
     4381 ttgcgcgctt cgttgaaaat tgccagttcg tggcgggtat ggtcgctgta tgtgatgaac
     4441 gggctgtaca gcggacgttg atggctcaat tcgcgcaaca gggcggcttg tttttgctct
     4501 tcgttcaggc tgttgtagtc ttccaagcct gcgtgttgga aaagctcggc aaccacatcg
     4561 gcgtgtttgc ctgcgtgttg gcgcaggtcg agcggcatca tgtggaagcc gaacacggac
     4621 acggaacgga tgaggtctgc caaacggcct tcagcaagca ggcggctgcc gttgtcgata
     4681 agggaacgtt gcaatttttt caaatcatcg agaaattctt gtgccgaagc atacggctcg
     4741 agaaagccga atttgcagcc catgcccaaa ccgagcgcgc gcgctttgcc catagcgcgc
     4801 gccatgatgt aagcaatggc gcggcggtag ggttcttcgg tgcgagcgat ttcttcgtca
     4861 ggcgagaggg ctgccagtgc cattacgtcg tcgttgactt tgacgcggcg gatggagagc
     4921 ggcagttcgc ggtaaagttt gtcgagttcg ccacggtaaa aacggaacac ggcatcggcg
     4981 tggcggcgga aggcaaaacg caaggtttcg gcagaaacga acggattgcc gtcgcggtcg
     5041 ccaccgatcc agccgccgat tttgaggatg ttcggaacgc agacatcggg atagaccatc
     5101 tgaaagtcgt gttccatatt gcggtagagc ttgggcaggg cttcgaaaaa gctcatcggg
     5161 aagatggaca cgccgttgtt gatttcgtcg ttgacgctga gtttgtggcg gcgcgtttcg
     5221 ctggtctgcc acaagcccag caggatagtg tcgatttcgc ggcgcagccg tgccagcgcg
     5281 tcggcattgg tgcagcgttc gcgttgcggc aacagtgcgc ggatgcggcg gttgaagctt
     5341 aagacggttt ggcgttgcac ttcggtcggg tgcgcggtca aaacggcggt aacggacgta
     5401 ttgtccaact gccgctgcac cgatttgccg tcggctttcc ccgctttgag cctgcggacg
     5461 gtttccgtca ggctgccttc cgcgccgccg cgtccggctt cttcgtggat ttggcggcgg
     5521 cgttcgtggt gcacgtcttc ggcgatattc aaaatctggg cgaacaggcc gcaggccaag
     5581 gttaaatcgt gggtttgttg ttcgtccaat tgcggcaata ctttttcaat caatgccgcg
     5641 ctgtcgtcgg aagtggacaa gagtttgacc gtttcgacaa ccaatggcga ggcttcttcg
     5701 tgcaggaggt tgaacaggga ttgtttcaga aattccgcgt ccgccgccaa agccgcgtcc
     5761 tttggattgt tcagaatatg cagttgcatg atttttctct cattgccgta aatactgtaa
     5821 atgtacctca aatgccgcat ccgtgccaaa ccgttcacac tttaaccgcc cgtgtcccga
     5881 aatgccgtct gaagttgaac gccgcccgac ggcagcgtta caatcgccca caactgtttt
     5941 ttccgaacat catcatgacc acgaccgaac acgacaacga cgatgcattc ctgctgcggt
     6001 acagccgcca catcctcttg gacgaaatcg gcatcgaagg gcagcagaaa ctttccgccg
     6061 cgcatatttt ggtcgtcggc tgcggcggtt tgggtgccgc cgccctgccc tatctcgccg
     6121 cttccggcat cggcacgctg accatagccg attccgacac ggtcgagctg cacaacctgc
     6181 aacgccaagt cgcatttgac gagggcgatg tcggcaagcc caaagccgaa gccttggcag
     6241 accgcctgaa acgcatcaac cataacgtcg atgtccgcac cgtcaacgaa aaactcgacg
     6301 gctgccgcct gaccggtttg gttcaaaccg ccgacatcgt tttagactgc tgcgacaact
     6361 acgccacgcg ccaagccgtc aaccgtgtct gcgtacaaac gaaaacaccg ctggtttcag
     6421 gagcagcggt gcgctttgaa gggcaacttg ccgtgtaccg tcccgacttg cccgactcgc
     6481 cgtgttacgc ctgcctgttt gacggcggat cggcttcaga cggcatctgt tccctcttcg
     6541 gcgtgttctc gccgctggtc ggcatcatcg gcagtaccca agcggcggag gctctgaaaa
     6601 tcctgctgga tgcgggcgaa ccgtcgcacg gcaggctggc ggtttaccgt gccttggaag
     6661 ggggctggca atatttcgac ctgccgcgca accccgaatg cccgatttgc ggcgcggaac
     6721 ggtaaaaccc tgccgccgtt tcagacggta tccaaacgga tgcggcggaa cggttttaaa
     6781 aatttaaaaa tttacatttc tttgcaaaaa aaataaactt accttataat tgcagttgtt
     6841 ttagcaatgt ctgtttcgca gactcattga gtaagacgtt ttccccgtaa tgtgtttggc
     6901 cgtctgtccc ctttgggttc ggacggcttt tttttggctg tgtatgaata cccggttggt
     6961 tttatctgtt tacaggggaa aaccgcttat ttccgttcgg gcggaaaacg gttccatcgg
     7021 ataaaaggca ttttgtccga ctgattaaag ttacatctta ggtttgcatc ggcaaaatat
     7081 tcaaacacag ccttttttaa ggaaatccgg atacggcggc gcatcaataa tgcggcggaa
     7141 tctcgtcgcg cagggaatac ggctcttgcg cgtcgggatt cctgtcctgc attttttgat
     7201 acagcagcct caactgagcc tgctgcaaat ccagcgtctg ccgcaattcc gccaccatcg
     7261 cgttcaggcc ggcgattacg tcctcctgaa gcgcggattg gatttccagt tcgacaatac
     7321 ggcgttccaa ctcttgaacc gcgtccattt acagcaccat cgcggcaatc cagccggcaa
     7381 tcagcagcgg gatgttgtag tggatgaagg tcgggataac ggaatcgcgg atgtggtcgt
     7441 gctgcccgtc ggcgttcagc cccatcgtcg ggcccagcgt ggaatcggac gcaggcgaac
     7501 cggcatcgcc caacgccccc gccgtgccga caatggcgac ggtggcaagc ggcgaaaaac
     7561 ccaaaccgac acacaaaggc acataaatcg cggcaataat cggcaaagtg gaaaaggacg
     7621 aaccgatgcc catcgttacc aaaagcccca ccaccagcat cgccaacgcc gccatacctt
     7681 tgctgttgcc gaatatcgcc atactgcttt ccaccagcgg ctgaatatgc ccggtcgcat
     7741 tcatcacggc ggcaaaaccc tgcgcggcaa tcataatgaa gccgaccatc gccatcatct
     7801 tgatgccttc gccgaatacg tcgtttgcct tgtcgcggtt gatgacgccc aacatcataa
     7861 atacggcgaa accgagcatc gcgcccaaca ccagcgagtc ttcatacatc aactggatgg
     7921 caaagcatac ggcaatggcg gcggcggcgg ccaggctgcg gtaggcggac ggctgcggac
     7981 ggtttgccgc atcggcgttg cccgccgtat cggcattgtt gctttggtac aggcgcggtt
     8041 tgcggtaatg gacaaacacc agcaggagtc cggccagcat tcccaacgcg ggaatcgcca
     8101 ttgccgccat tacgttgatg tttttcacat caagctgcgg cgcggcggaa tggatgttgc
     8161 ccaacaggat ttcattcaaa aaaatcgcgc cgaagccgta aggcaggaac atataagtcg
     8221 taaccagccc gaaagtgatg acgcacgcaa tcaggcggcg gtcgattttc aggcggttga
     8281 acaccaaaag cagcggcgga acaatcatcg ggataaaggc gatgtggatg gggatgatgt
     8341 tctgactcat aatgcccatc acaaggatga tggaaagcag cagccatttg accgcgccct
     8401 cgcccgaacg cacgctgtcg ggcataccgc cccggttcag cttgcggacg accgcgccgg
     8461 caagctgttg cggcaggccg gaatgggtaa tcgccattgc aaacgcgccg agcatcgcat
     8521 aagaaagcgc aatcttcgca ccgccttcca aacctttgtt gaacacgggg ataatccccg
     8581 cctgactgac ctgtcccgcc gcatcggcaa tgttttgcag cggcataccc gccaccgcgc
     8641 cgccgacaaa cgcgccgacc gtcaggctca ataccacgtg cacgcgcgac agcgacagca
     8701 ccagcataac gattacggca actacgactg cattcattgt ttatcgctcc aaacctataa
     8761 atgtttacat atcgaaacac atcataaccc aataatggaa aacccgccaa ttttgcaaac
     8821 aattatttca aatgcttcat atacttcccc agcgtaaccc tgtccaaacc cgccaagtcc
     8881 ggcagggttt ccactcctga aaaaccattc tccgccaaca cgccgcgcac cgccgcgccc
     8941 tgatcgaaac cgtgttccaa caacaaaaaa ccgccttccg ccaaacggtc gggcgcgcct
     9001 tgcgccaagg tgcggatgca gcttaggccg tctgaaaagt cggtcagcgc gatttgcggc
     9061 tcaaaccgca aatcgccttg caacaaatgt ttatcgccgt tttcgatata gggcgggttg
     9121 gacacgatga tgtcccattt cccttcagac ggcatatcgg tgtcgaacca cgaaccgtgt
     9181 gcaaattcga cccgcgcgcc caaatctgcc gcatttttcc gcgccgtttc aagggcgggc
     9241 gtgctgatgt cggatgcgcg cacaaacgca tcggggcgtt cgagtgcgac ggttacggca
     9301 accgcgccgc tgcccgtccc caaatcccac acgcgcccgt tttcgggcag gcgcgccaat
     9361 acggcttcga ccaagtgttc ggtttcgggg cgcggaatca gcacgctcgg attgactgta
     9421 aagcgtctgc cataaaattc gcgcacacct aaaatatagg caaccggctc gccgttcaga
     9481 cggcgttgcg ccagcctgtc cgcccgctgt cggacttcgt ccggcatttc ttccccgccc
     9541 cgcgtcaaca actgcacgcg cgtatattcc gaaacatatt gcagcagcat tcttgcttca
     9601 attttaggca gttttgacaa gcccaaccat tcgtcaaacg tcatttttta tcccgtctgc
     9661 cgccgatgcg gcttttcttt ccttattctt tccggcaaac gtaccgatgg tggcaaccgc
     9721 aaatgcggca taccacaaat aaaatcctgc accgtagcgc acaatatccg atgtattccc
     9781 tgcttcatcg acgtatacgg ctttcacact gaaagccacc aacgccaagc cccaaagtgc
     9841 cgcatggaca ggcacgacct tcttccgcaa cgccagcaaa acaatggccg ccaaccaaac
     9901 ataattcgca tagaccgcac aatacctgat atccaaagaa gcaaatatcg accccaaaat
     9961 caaaacggtc aaaccctcca tgcttccatg attgcccaaa taaaatgcaa cattggataa
    10021 agacgctatc cacagggcaa ccgacaccag caacatcact atgggaaaac ttgatttccg
    10081 attctgttcc tgcatggttt tatcctaatg taaaaggccg cctgaaaacc tttcagacgg
    10141 catcgtgccg gattccgcgt cagattgcgc tgccgccgac ggtcagtccg gcatcaatcc
    10201 gcaaagtcgg ttgccccacg ccgacgggga cgctctgccc ttctttgccg cacacgccga
    10261 caccgctgtc gagcgcagta tcgttgccta tcatggaaac gtgtttcagc acttcggggc
    10321 cgttgccgat aatggtcgcg cctttgacgg ggtattgcag cctgccgccc tccacccacc
    10381 acgcttcgga cgcgctgaac acgaatttgc cgctggtaat gtccacctgt ccgccgccga
    10441 agttgacggc gtaaatgcct ttgtcgatgg acgcgatgat ttcgtcgggt tcgtagccgc
    10501 cgttttccat aaaggtattg gtcatgcgcg gcatagggac ggaggcgtag ctttcgcggc
    10561 ggccgttgcc ggtcgattgc gtgcccatca ggcgggcatt ggtttcgtcc tgcatataac
    10621 cgaccaagat gccgtcttca atcaatacgg tgcggcgggt ttcgttgcct tcgtcgtcga
    10681 tgttgagcga accgcgccgg ccggcaatat cgccttgatc cacaacggta acgcctttgg
    10741 cggcgacgcg ctcgccgatt cggccggaaa agacgctggt tcccttgcgg ttgaaatcgc
    10801 cttccaaacc gtgtccgacc gcttcgtgca gcaacacgcc cggccagccg ttgcccaaaa
    10861 cgacggtcat ttcgccggca ggcgcaggac gggattcaag attggtcagg gcttgtttga
    10921 cggcggaatc gacaaaccga tgcaccaagt tttcatcgaa ataagccaag tcgtagcgtc
    10981 cgccgccgcc cgcgctgccc tgttcgcggc gttcgccctg tttggcgata acggtaacgt
    11041 tcatgcgcac catcgggcgg atatcggcag cgtgtttacc gtccagacgg gcgaggtaaa
    11101 ccatgtcgta ttcgcaggtc aaaccggcca tcacttgcac gatgcgcgga tcggcggctt
    11161 tggcgattgc ttccactttg ttcaacagcg cgactttggc ggcggaatcg aggccggcaa
    11221 tggggtcgga cgcggaacaa accggcttgc cgcgcgtttc agacggcatt ttggcggaca
    11281 ccttgccgcc tgccgcccca atcgcgcgga cggcgcgggc ggaacggttt atcgaatcga
    11341 tgcacaggct gtcggcgtag gcaaaggcgg ttttatcgcc cgaaacggcg cgcacgccca
    11401 cgccctgatt gatttggaag ctgcccgatt tgacgatgcc ctcttccaaa tgccagcttt
    11461 cataagcggt gcgctggcag tagatgtcgg cgtaatcgac gtggtgcgcg ccgatgatgc
    11521 acaggctttt ggcgagcagt tcgggggaaa ggcggttggc ttcgagcagc cgcgcctgta
    11581 cggcggaata ggtcggatgc atagtgtcgg cgcataaaaa atcaggggct tgattatacg
    11641 gcatttgtta tggaaatgcg ccgtgccgcc gaaaatgtaa gaattttgcc aacgcacgtt
    11701 gctttgtgta aacttaaagc tttcttgtcg gagtgccgcc gccgggcggc tgagattgcg
    11761 aaagcagaat ccgtagaacc tgtcggggta atgcctgcgt aggaaacaaa ccgtcaaatg
    11821 ccttatcagg cttccgttcc cttttccgca cttccccgcc caccttcctt ctttttaaag
    11881 gacgtcatat gtcgggcaat gcctcctctc cttcatcttc cgccgccatc gggctgattt
    11941 ggttcggcgc ggcggtctcg attgccgaaa tcagcacggg tacgctgctt gcgcctttgg
    12001 gctggcaacg cggtctggcg gctctacttt tgggtcatgc cgtcggcggc gcactgtttt
    12061 ttgcggcggc gtatatcggc gcactgaccg gacgcagctc gatggaaagc gtgcgcctgt
    12121 cgttcggcaa acgcggttca gtgctgtttt ccgtggcgaa tatgctgcaa ctggccggct
    12181 ggacggcggt gatgatttac gccggcgcaa cggtcagctc cgctttgggc aaagtgttgt
    12241 gggacggcga atcttttgtc tggtgggcat tggcaaacgg cgcgctgatt gtgctgtggc
    12301 tggttttcgg cgcacgcaaa acaggcgggc tgaaaaccgt ttcgatgctg ctgatgctgt
    12361 tggcggttct gtggctgagt gccgaagtct tttccacggc aggcagcacc gccgcacagg
    12421 tttcagacgg catgagcttc ggaacggcag tcgaattgtc cgccgtcatg ccgctttcct
    12481 ggctgccgct tgccgccgac tacacgcgcc acgcgcgccg cccgtttgcg gcaaccctga
    12541 cggcaacgct cgcctacacg ctgaccggct gctggatgta tgccttgggt ttggcagcgg
    12601 cgttgttcac cggagaaacc gacgtggcaa aaatcctgct gggcgcaggt ttgggtgcgg
    12661 caggcatttt ggcggtcgtc ctctccaccg ttaccacaac gtttctcgat gcgtactccg
    12721 ccggcgcgag tgcaaacaat atttccgcgc gttttgcgga aacacccgtc gctgtcggcg
    12781 ttaccctgat cggcacggtg cttgccgtca tgctgcccgt taccgaatat gaaaacttcc
    12841 tgctgcttat cggctcggta tttgcgccga tggcggcggt tttgattgcc gactttttcg
    12901 tcttgaaacg gcgtgaggag attgaaggct ttgactttgc cggactggtt ctgtggctgg
    12961 cgggcttcat cctctaccgc ttcctgctct cgtccggctg ggaaagcagc atcggtctga
    13021 ccgcccccgt aatgtctgcc gttgccattg ccaccgtact ggtgcgcctt ttctttaaaa
    13081 aaacccaatc tttacaaagg aacccgtcat gacccgtatc gccatcctcg gcggcggcct
    13141 ctcgggaagg ctgaccgcac tgcagcttgc agaacaaggt tatcagattg cacttttcga
    13201 taaaggctgc cgccggggcg aacacgccgc cgcctatgtt gccgccgcca tgctcgcgcc
    13261 tgcggcggaa gcggtcgaag ccacgcctga agtcatcaaa ctgggcaggc agagcatccc
    13321 gctttggcgc aacatcagag gccatctgaa aacgcctgcc atgatgcagg aaaacggcag
    13381 cctgattgtg tggcacgggc aggacaagcc attatccagc gagttcgtcc gccatctcaa
    13441 acgcggcggc gtggcggatg acgaaatcgt ccgttggcgc gcagatgaaa tcgccgaacg
    13501 cgaaccgcaa ctcggcggac gtttttcaga cggcatctac ctgccgaccg aaggccagct
    13561 cgacgggcgg caaatattgt ctgcacttgc cgacgctttg gacgaactga acgtcccctg
    13621 ccattgggaa cacgaatgcg tccccgaagg cctgcaagcc caatacgact ggctgatcga
    13681 ctgccgcggc tacggcgcaa aaaccgcgtg gaaccaatcc cccgagcaca ccagcaccct
    13741 gcgcggcata cgcggcgaag tggcgcgggt ttacacaccc gaaatcacgc tcaaccgccc
    13801 cgtgcgtctg ctccatccgc gttatccgct ctacatcgcc ccgaaagaaa accacgtctt
    13861 cgtcatcggc gcgacccaaa tcgaaagcga aagccaagcc cccgccagcg tgcgttcagg
    13921 gttggaactc ttgtccgcac tctatgccat ccaccccgcc ttcggcgaag ccgacatcct
    13981 cgaaatcgcc accggcctgc gccccacgct caaccaccac aaccccgaaa tccgttacaa
    14041 ccgcgcccga cgcctgattg aaatcaacgg ccttttccgc cacggtttca tgatctcccc
    14101 cgccgtaacc gccgccgccg tcagattggc agtggcactg tttgacggaa aaaacgcgcc
    14161 cgaacgcgat gaagaaagcg gtttggcgta tatccgaaga caagattaaa gccgcccgaa
    14221 aggacacctt atgaccttcc cgcccctaaa atccccgctc aaattctacg ccgtcgtccc
    14281 caccgccgat tgggtggggc gcatggtcaa agcaggtgcc gacacggtgc aactgcgctg
    14341 caaggccctg cacggcgatg aattgaaacg cgaaatcgcc cgctgcgccg cagcctgtca
    14401 gggcagccgt acgcagcttt tcatcaacga ccactggcgc gaagcaatcg aagcgggcgc
    14461 gtacggcgtg catctcggac aagaagacat ggacaccgcc gaccttgccg ccatcgccgc
    14521 cgccggtttg cgcttgggtt tgagtacgca ctccgttgcc gaactcgacc gcgccctgtc
    14581 cgtacaccct agctacatcg ccagcggcgc gattttcccg accacgacca aacaaatgcc
    14641 caccgccccg caaggcttgg acaaactgcg cgaatacgtc aaacaagcag gcggcacgcc
    14701 cgtcgtcgcc atcggcggta tcgacttgaa caacgcccga gccgtactcg ccaccggcgt
    14761 ttcctcactc gccgccgtcc gcgccgtaac cgaagcggca aatcccgaag cggtggttaa
    14821 agcgtttcag gctttgtggg atggataaaa ccgaaagaag aaaattcaat tgccgtgtcg
    14881 gcaaaactta gcccgttatc gcaaacatac ttaactttaa atgtggcata tcatcaaatt
    14941 ccgtcattcc cgcgtaagcg ggaatccgcc ttaaaacttg agaaaccatc atttgaaaaa
    15001 cagtttccga atttcaaaaa tggattcccg cccgtgcggg aatgacggca accggtcagt
    15061 tgcgtatcaa aaaataaagt aattcggc
//
LOCUS       DAWGJV010000037        14896 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWGJV010000037 DAWGJV010000000
VERSION     DAWGJV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.037"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(116..3601)
                     /gene="smc"
                     /locus_tag="WHQ94_000830"
     CDS             complement(116..3601)
                     /gene="smc"
                     /locus_tag="WHQ94_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ2128144.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHASDGLSDDIATLQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            complement(3680..3826)
                     /locus_tag="WHQ94_000831"
     CDS             complement(3680..3826)
                     /locus_tag="WHQ94_000831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128145.1"
                     /translation="MSWRIITRPAAGQYGKCRAKHRALAEPGFGALKMPSEGFRRHRG
                     CGMR"
     gene            3850..4572
                     /gene="lpxH"
                     /locus_tag="WHQ94_000832"
     CDS             3850..4572
                     /gene="lpxH"
                     /locus_tag="WHQ94_000832"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ2128146.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4841..6427
                     /locus_tag="WHQ94_000833"
     CDS             4841..6427
                     /locus_tag="WHQ94_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ2128147.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6595..6798)
                     /locus_tag="WHQ94_000834"
     CDS             complement(6595..6798)
                     /locus_tag="WHQ94_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ2128148.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6914..7411
                     /locus_tag="WHQ94_000835"
     CDS             6914..7411
                     /locus_tag="WHQ94_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ2128149.1"
                     /translation="MTALTLPEDILQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADEGRDIPSLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIRSNWETFA
                     HIGQA"
     gene            7493..8686
                     /locus_tag="WHQ94_000836"
     CDS             7493..8686
                     /locus_tag="WHQ94_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ2128150.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIATEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9089..10024)
                     /gene="hemC"
                     /locus_tag="WHQ94_000837"
     CDS             complement(9089..10024)
                     /gene="hemC"
                     /locus_tag="WHQ94_000837"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ2128151.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10143..10658
                     /gene="ybeY"
                     /locus_tag="WHQ94_000838"
     CDS             10143..10658
                     /gene="ybeY"
                     /locus_tag="WHQ94_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ2128152.1"
                     /translation="MKRAKQYPFLSLQRQRFHLNFENASSTANLPSERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10660..11484
                     /locus_tag="WHQ94_000839"
     CDS             10660..11484
                     /locus_tag="WHQ94_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ2128153.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11613..12992)
                     /gene="nhaC"
                     /locus_tag="WHQ94_000840"
     CDS             complement(11613..12992)
                     /gene="nhaC"
                     /locus_tag="WHQ94_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ2128154.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13282..14505)
                     /locus_tag="WHQ94_000841"
     CDS             complement(13282..14505)
                     /locus_tag="WHQ94_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ2128155.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3521 a         4178 c         3824 g         3373 t
ORIGIN      
        1 tgagctaagg cgagccaacg ctgtaccgat ttaaatttaa ttcactatac cgtctgaaaa
       61 cctgtatcgc aacacgtcat taaaaacggc aagattcagc cgttctgcaa cccgttcaaa
      121 cggcttccgc catttccaac gcctgcttga tgtccacggc gacgacgcgc gatacgcctt
      181 tttcctgcat ggttacgccg accagctgct ctgccatttc catggtcagg cggttgtggg
      241 agatgtagag gaactgggtt tgcgccgaca tttctttgac caggttgcag aaacgcgagg
      301 tgttggcgtc gtccagcggg gcatcgacct cgtccaaaag gcagaacgga gcggggttga
      361 ggctgaacag agcgaacacg aggctcatgg cggtgagggc tttttcgccg ccggagagga
      421 ggtggatggt gctgtttttc ttgccgggcg gacgcgccat aatggacaca ccggcggtca
      481 gtaggtcgtc gcctatcatt ttgagagtgg cttcgccgcc gccgaacagg gtcgggaaga
      541 aggtttggac tttgccgttg acggcgtcga aggtttcttt gaaacgcgct ttggttttgt
      601 cgtcgatttg ggcgatggct tcttccaaaa tggtgatggc ggcctgcacg tcttcgcttt
      661 ggctgcggta gtagccgtcg cgttcgcgcg cttcttcgag ttcttgcagg gcggcgaggt
      721 tgaccgcgcc gagggcttcg atttgttgcg agaggctgcc gatgctgctg ttcaatactt
      781 tcggcgattc tttcgccaac gcttcgagcg catccaaatc ggcggcgcgt tcggtcaggt
      841 tttgatggta gcgtttggcg ttgatcaggg cttcctgttg ttgcaacagg gcggtttggg
      901 tggcggcctg aagctgtggc aactgggttt gcagggtttg tacgcgggcg tattgttccc
      961 taccctgctc ctgaatctgc gtgagttttt cctgcacggc gatgtattct tcgtccagcg
     1021 tgtgtacgga ttcggtcagc tcgtcgagct tgatgtgctg ctcgtcgttt tggaactcgg
     1081 tttcataggc gaggacaagc tcttgctggc gttcctgcca gtctagggtt tgctgttcga
     1141 gtcgggcgat ttgctgctgg tagttttgtt tttgctggtt gagtttgtgg acggcgactt
     1201 cggcaagccc atattggcgg ttggcttcca gcagggcaag ctgcgcctgt ttcagacggc
     1261 cttgctgctc ttggcggctg tgtgcggcgg tttgctgctg gtgttcgagt tcggcggcgg
     1321 cttcctgcaa agtagcaatg tcgtctgaaa gcccgtcgga cgcgtgttgc aacacggtct
     1381 gctcttccgc caactgcgcc agttcgcgct cgatgtgttc gcggcggatt tgcccttggt
     1441 tggtacgcgc cagaagttcg gcggcgcgtt gctgcgcttg gctgtattgg cgcgtgtgct
     1501 gctgttgctg ctgcatcagg tttttatgct gcacttcgga cgagcgcacg gcggcttccg
     1561 cctgtttgaa cgcggcttcg gcggcagaaa gttcgggggc gaggctttcc agttcggaag
     1621 cgatgccgtc gaggcgcgct ttttgggcaa tcaagctttc ctgcgcgggc ttggcataga
     1681 gcagaacgct gactttatcg acctgatggc cttcgggtgt gagccagatt tggtgtgcgc
     1741 ccaaatcgct ttgatgcgcg agggcgtagc tcaaatcggg cgcgcacaat acgccgtcga
     1801 gccagtggtg caatgccgtc tgaaacggcg gctgcgcttg gatttggttc agcaatgcct
     1861 gtacgggcag ggattttttg atgccgccgg agagaccgtc tgaaagccac gccgcctgcc
     1921 cctgcggcaa aggttcgggc ggtacgaaac cttgcggcac gtcgcgggcg tgcaggcgtt
     1981 cggcaaggat gacggacagg gcgtgctgcc actcagcagg cgcggtaatg tgttgccaca
     2041 gttgcggcgc ggcggcgtgg ccggttgcct gccagaaatc ggctgcttcc tgctgttgcg
     2101 acaggatttg cgacaacgcc tgctgctgcg cctgcaaggt gatatgttgc tgcttcaggc
     2161 tttggaagcg gtttgaggcc gtctgaaacg cctcgcgggc ggcgtgtaag gcttcttcgg
     2221 cggcgatgat ttgttcttcg taatgctctt gccggctttg caacaaggca gcggcctcct
     2281 gcgcggcggc ggtttccgct tcgtcgggca ggttcagggc ttggttctcc tgtttcagac
     2341 ggcctttgcg ctcttcgtgc ttggcaacgg tttgttcggc atgggcaagc tgctgctgct
     2401 tcaacgccag ttcgcggcgg atgcggtttg cctcgtcctg ctgggtttgg aaggcggcat
     2461 tgagcgcggt ttgggtttct tccaattcgg gcagacgctc ttcgtgttcg gcaacctgca
     2521 tcgcccactc cgccagctcg gtttgctttt cttcgacgta taactcgttt tcttcaagct
     2581 gcacgcggat ttgctgctgc tcttgatgaa tacgctgcat ctgcgcctgc gccgcctgct
     2641 tgtcgcgttc gatgcgttgg tgcaggtttt gctgatggcg gatttgttct tccaaacggg
     2701 caatctgctc gcgcaacacg ccgcgtttgt tgctcaattc atgcaccgcc tgttgctgcg
     2761 actgttcggc ggtctgcaag gcgtgtactt cgtcgtttaa cgcctgaacc tgcgcggcgg
     2821 tttcgtcctg ctgcgcctgc aaagactgat gctgctcggt cgccttgtcg gcggcggcaa
     2881 gcgattgctg ccattgggcg taatcgagca aatcctgttg ccggttcaac tgcgcggtca
     2941 gggatttgta gcgttcggcg gtttccgcct gtttctccag cttttccacc tgacgcgcca
     3001 attcgttctg caaatcgccc aaacgctgca aatgctcgcg cgtgtctttc agacggcctt
     3061 ccgtctcctt gcggcgttcc ttatatttgg acacacctgc cgcctcctcg atataggcac
     3121 gcaactcctc cggccgcgct tcgatgatgc gcgaaatcat cccctgctcg ataacggcat
     3181 aaccgcgcgc acccacgccc gtacccaaaa ataaatcggt aatgtcgcgg cggcgcacgg
     3241 tctgattgtt gatgaaataa gtcgattcgc cctgacgcgt cagctgccgc ttgatgctca
     3301 cctcggcata ctgtccccac gcaccctgca aactgtggtc gctgttgtca aacaccagct
     3361 ccaccgaagc cctcggcgca ggacggcgcg tcgccgcacc gttaaaaatc acgtcctgca
     3421 tactctcgcc acgaagctgc ttcgccgaag cctcacccaa cacccagcgc accgcgtcaa
     3481 tcacattcga cttgccgcaa ccgttcggcc cgataaccgc gacaagctgc cccggaacat
     3541 gaatcgtggt cgggtcggta aaagatttga agccggagag tttgatgtgg gtcaggcgca
     3601 taatggtcgg aaaaaataaa aaagaagcgt attttaacgg aaatcccgcc gcaccacccc
     3661 atatcttgcc ggcaaaacct taccgcatcc cgcacccgcg atgccgtctg aagccttcag
     3721 acggcatttt taacgcgccg aaccccggtt ccgccaatgc ccgatgcttg gcgcggcatt
     3781 tgccgtattg ccccgccgcc gggcgcgtta taatccgcca cgacatccgc ccgatttgga
     3841 aacctgccta tgaaacccgc ctatttcatt tccgacctgc atttgagcga aaagcagccc
     3901 gaactgaccg cgctgttgct gcgtttttta cgttcttccg ccgccgggca ggcgcgggcg
     3961 atttatattt tgggcgattt gtttgatttt tgggtgggcg atgacgaagt ttccgagttg
     4021 aatacttcgg ttgcgcggga aatcaggaaa ttgtccgaca aaggcgttgc cgtgttcttc
     4081 gtcaggggca accgcgactt cctgatcggt caggattttt gccggcaggc gggtatgacg
     4141 ctgctgccgg attactcggt tttggacttg ttcggctgca aaaccctcat ctgccacgga
     4201 gacacattat gcaccgacga caaggcatac caacgtttcc gcaaaatcgt gcatcggaag
     4261 cggctgcaaa aactgttcct gatgctgccc ctaaagtggc gcacgcgcct tgctgccaaa
     4321 atcaggcgtg tcagcaaaat ggaaaaacag gtcaagcccg ccgatattat ggatgtcaat
     4381 gccgccttta ccgcgcggca ggttcgcgcc tttaacgcgg aaaggctgat acacggacac
     4441 acccaccgcg agcatatcca tcacgaaaac ggctttaccc gcatcgtttt gggcgactgg
     4501 cataacgact atgcttcaat cctccgcgtg gacggggacg gcgcggtatt cgtgccgctg
     4561 gaaaaatgct gaaaatgccg tctgaagcct ttcgggcggc tttttttaca cgcccctttc
     4621 ccacactccc tccgccgcat catcaccatc cttaacccgc attaatagcc attatcgata
     4681 aaatgcttga ccgattacat aagattacgt aaagtgtgca aacgcataat aattggtctt
     4741 accaatttat cttttaacca aaatatcggg tgtacgggat ttgaacgtct ttccaaacct
     4801 cgacgcgcct tatgatgcaa atgcctaaaa aggattcgtt atggcacttt tcctcagcat
     4861 attccccatc gtcctgctga tttggctgat ggtgaaaaaa aacagtatgc cctcctacgt
     4921 cgcactgccg attaccgcag tgctgattta cgccatcaaa cttttctact tcggcgatgc
     4981 gggcatgctg ctcaacgcca ccgccgcttc cggcctcgtc aaaacgctca cgccgattac
     5041 cgtgattttc ggcgcgatta tgttcaaccg tatgatggaa accacgggct gcattgatgt
     5101 catccgcaaa tggctggcga ccatcagccc caaccccgta gcgcaactga tgattatcgg
     5161 ctgggctttt gcctttatga ttgaaggcgc atccggcttc ggcacgcctg ccgcgattgc
     5221 cgcgccaatt ctgatgagct tgggcttcaa cccgttgaaa gtggcgattt tcactttggt
     5281 gatgaactcc gtccccgtct ctttcggcgc ggtcggtacg ccgacctggt tcggtttcgc
     5341 accgctgaac ctgagtgccg aagacatcct cgccatcggc aggcagaccg gcgtaatgca
     5401 cttcttcgca ggtttcgtca tccccgtcat cggcttgggc ttcatcgtac cttggtctga
     5461 aatccgcaaa aacttgggct tcgtcgccat tgccgtcttc tcctgcacca ttccttatgt
     5521 cgcattggcg atggtcaacg aagaattccc gtcgctcgtc gccggcgcga tcggcctgat
     5581 ggtgtccgta ttcgccgcca accaaggctg gggcttgagc aaagaccacg ccaaagaccc
     5641 gaatgccgaa aaagtgccgt tcgcccaagt cgccaaagca ctcgcccctt tgggtatgct
     5701 gatcggcatg ctggtggtta cgcgcatcaa acagctcggc atcaaaggca ttttgaccag
     5761 caaagaagaa tggttcagct tccaactgcc gtttgatttg tccaaaatca ccgtcagcga
     5821 ctccctgacg attaccttcg gcaatatttt cggacaagat gtcagcgcgt cttaccaaac
     5881 gctgtacgtc ccggcttgga ttccgtttgt gctgaccgtt tggatttgca tcctgctgta
     5941 taaaaccaaa ttcaaagatg cctggacgat ttattccgta actttcaatc aaaccaaaaa
     6001 accgctgctt gccctgatgg gcgcgctgat tatggttcag ctgatgctgg tcggcggcga
     6061 caattcgatg gtgaaaatca tcggtaagga atttgccgca atggcgggcg aacactgggt
     6121 ttatttctcg ccgtatctgg gcgcgatcgg tgcgttcttc tccggctcca acaccgtgtc
     6181 caacctgact ttcggcccga ttcagcagca aatcgccttg gataccggcc tgtccgttac
     6241 cctgattctg gcgttgcagt ccgtcggcgg cgcgatgggc aatatggtgt gcctcaacaa
     6301 catcatcgcc gtatgtaccg tattggatgt gaaaaattcc gaaggtgcga ttatcaagaa
     6361 aaccgttatc ccgatggcga tttacggcgt gattgccgtc gtcgcggcaa tgattttctt
     6421 cctctaggcg aaacgccacc atccatagcg gcagcacgcg gggaacggaa accgccgttc
     6481 cccgtctggg cggcgcgtcg ggcagaatcc cggtgccgga acagcccgcc ccgccaaaca
     6541 aatgccgtct gaaaccggaa aaggcttcag acggcatttt tccgccgttt gcattcaggc
     6601 gtaatgatga tgcggcatct tgccgtcaag gcggtagcgc gtgccgcaat acgggcaggc
     6661 aacgctgccc gattctcctt cgcacaaagg caaaaacact ctcggatgcc cgttccactg
     6721 ctcgttgtcg ggtcccgagc aatacagcgg cagattttcc ggcaacacgg aaatttcctg
     6781 cagattcaga ttgtccattt gattttcctt tgcgtggttg gcgtgcgccc attttacgcc
     6841 atcggcaggc taaaggatat tttcggcgca aagccgcaat ccgctataat ccccactttt
     6901 cagacggcat accatgactg cgcttaccct tccggaagac atcctccaac aagaaccatc
     6961 cgttctgctc tataccctcg tttccgccta cctcgaacac accgcccaaa ccggcgacga
     7021 atccctctcc tgcctgtccg acgaccagca cacgctgacc gcattctgct acctcgacag
     7081 ccaagtcgaa gaaggcggct tcgtgcaact catcgcctcc ggttacggcg aatatatttt
     7141 ccgcaacccg cttgccgaca gcctgcgccg ctggaaaatc aaagccgtgc cgaaagtctt
     7201 ggacaaagcc aaagccctct acgaacaaca cggcgaaacc atcgaaacgc ttgccgacga
     7261 agggcgggac atcccttccc tgcgcaaaca gttccccgac tttgaagaat gggacggcgc
     7321 atactacgaa gccgccgaac aagacctgcc cctgcttgca gaacacatac ggtcaaactg
     7381 ggaaaccttc gcccatatcg ggcaggcgtg attgcgtctg tttccagccc gtgtaaaaca
     7441 gcgtaaaatc ggcaaacccg aatgaccttc cgtccccatc aaggagcaag ctatgttctt
     7501 caagcacatc gaagccgccc ccgccgatcc gattctcggt ttgggcgaag cattcaaagc
     7561 cgaaacccgc cccgaaaaag tcaacctcgg catcggcgtt tataaagacg catccggcgc
     7621 gacacccctc gtcaaagccg tcaaagaagc cgaaaaacgc ctgttggaaa gcgaaaccac
     7681 caaaaactac ctcaccatcg acggtgttgc cgactacaac gagcaaaccc aaatcctgct
     7741 gtttggcaaa gaccacgaaa tcatcgccag ccgccgcgcc aaaacagcgc aaagccttgg
     7801 cggtacaggc gcattgcgta ttgcgaccga gtttgccaaa cgtcagttga acgcgcaaac
     7861 catctggatt tccaatccga cttggccaaa ccacaacgcc atcgccaaag cggtcggtat
     7921 ccaagacaaa ccttatcgtt actatgatgc cgccaaacac ggtttggatt gggacggtat
     7981 gattgaagac ttgagccaag cgcaaaaagg cgacatcgtc ctgctgcacg gctgctgcca
     8041 caaccctacc ggcatcgacc ctacgcccga acaatgggaa actctggcaa aactttctgc
     8101 cgaaaaaggc tggctgccgc tgtttgactt tgcctaccaa ggcttcggca acggtttgga
     8161 agaagatgcc tacggcttgc gcgtgttctt gaaacacaat acagaattgc tgattgccag
     8221 ctcttattcc aaaaacttcg gtatgtacaa cgagcgcgtc ggcgcgttca ctttggtggc
     8281 cgaagatgaa gaaacagcag cccgcgccca cagccaagtc aaaaccatca tccgtacctt
     8341 gtattccaac ccggcttcac acggtgcgaa caccattgcg ctggtgttga aaaatgatga
     8401 tttgaaagca caatggattg ccgaactcga tgaaatgcgc ggccgcatca aagccatgcg
     8461 ccaaaaattt gtcgagttgc tcaaagccaa aggtgcaagc caaaactttg atttcattat
     8521 cgaacaaaac ggtatgttct ctttcagcgg attgactccc gaacaagtcg accgtttgaa
     8581 aaacgagttt gccatttatg ccgtccgctc cggccgcatc aacgttgccg gcattaccga
     8641 cgacaacatc gattatctgt gcgaaagcat cgtgaaagtc ttgtaaaaca aaagcgcata
     8701 cgaaatgccg tctgaacctt aaccagttca gacggcatct gttttttgtt aaaaaacaat
     8761 aaaccataac aatttcaaac aagctaaaaa catctataaa atcaacccaa tatgatgaac
     8821 tcaaataaaa aatacgaata tcatcattct caaattcaaa atttgaacgc cacagtatta
     8881 tccgaagcca caaaaacctt tccgccgtca ttcccgcgca ggcgggaatc cgggaaccca
     8941 atgctgcaag aattgattag aaacaactga aaccgagcaa actggattcc cgcctgtgcg
     9001 ggaatgacga aataaggcga gtaaaactga tgaaacaaat tgattaaaaa caataactta
     9061 tattatttta tttaaatttt cgataaaatc aattttccgt attcaatact gctccaatca
     9121 attcccgcgc accgtcgtcc gccaatttct tagcgacagc gcgtccgagc gcgtcggcat
     9181 attcggcagg ggcttgcgcg tccgcctgca aaatgaccga accgtcgggg tgtccgacca
     9241 gtccgcgcaa ggtcagcaag ccgttttctt ccgtgcaata tgcggccaaa ggcacttggc
     9301 agcttccgcc caaagcgcgc gccaaagcgc gttcggccgt aacgcaggcg tgtgtggtat
     9361 cgtggtttag cggttttaaa acctcataca aatcttcgcg gtgcgcggca atttcgatac
     9421 ccaatgcgcc ttgtccggcg gcaggcaggc tgtcggattc cgacaaaatc atgcggatgc
     9481 gtccgtccaa ttccagacgc tgcaaaccgg cggcagccaa gataattgcg tcgtattcgc
     9541 cgttatcgag tttagacaaa cgggtttgca cattgccgcg caaaggtttg ataagcaaat
     9601 gcggatagcg cgcacgcaac tgggcttcgc ggcgcaggct ggatgtgcct acgacggcgc
     9661 ctttgggcat ttcttccaaa cgcgcgtatt ggttggacac aaacgcgtca aacggattgg
     9721 cgcgttcgcc gatggctgca agcgcgaaac cttcaggcaa atccatcggc acatctttaa
     9781 tcgaatgcac ggccaagtca gcgcgcccgt catataaagc ctgttccaac tctttgacaa
     9841 acaagccctt accgccgact tttgacaaag ttttgtccaa aatctggtcg ccgcgcgtgg
     9901 tcatgcccaa aatctcgact tcgcaatcgg gatacagggc tttcagacgg ccttggatat
     9961 gctttgcctg ccacatggca agcaggcttt cgcgactggc gataacgagt tttttcgggt
    10021 tcataatggt tgcctgaaag atggaaaacg gcatgaagtc tatcatattt tgggcggtct
    10081 ttcagacggc atgagggtga atatgttata tttacgccta aaaaccaaat tttccggaca
    10141 acatgaaacg cgccaaacaa tatccttttt tatccttaca gcgacaacgt tttcatctga
    10201 actttgaaaa cgcttcttcc accgccaacc ttccgagcga acgcgatttc taccgctggg
    10261 cgtggtctgc cttgaaaaat gaataccgcc gcgccgacat cagcctgatt cttctggacg
    10321 aagaagaagc ccgcgcttac aaccgcgact accgcggcaa agattacgcc accaatgtat
    10381 tgagtttcgc gctcaacgaa ggcgaaatcc tgccctgcca agtttcggaa aaactgtacg
    10441 gcgatttgat tatctgcccg caagtggttt tgaaagaagc cgccgaacag ggcaaaacgc
    10501 ccgaacggca ttttgcccac ctgaccatac acggcgtttt gcacctgatg ggctacgacc
    10561 acatcgaaga cgatgaagcc gaaataatgg aagccgaaga aatccgcctg atgctggcgg
    10621 caggcttccc caacccctac caagaggacg aatattaaaa tggacggtac gcagtcgaaa
    10681 cccaagtttt tcgaacgcct gatctcccga cttgccggcg agcccgactc cgccgaagac
    10741 gtattgaccc tgctgcgcca agcgcacgag caggaagttt ttgatgcgga tacgctttta
    10801 aggttggaaa aagtcctcga tttttccgat ttggaagtgc gcgatgccat gattacgcgc
    10861 agccgtatga acgttttaaa agaaaacgac agcatcgagc gcatcaccgc ctacgtcatc
    10921 gataccgccc attcgcgctt ccccgtcatc ggcgaagaca aagacgaagt tttgggcatt
    10981 ttgcacgcca aagacctgct caaatatatg ttcaaccccg agcagttcca cctgaaatcc
    11041 gtcttgcgcc ctgccgtttt cgttcccgaa ggcaaatctt tgaccgccct tttaaaagag
    11101 ttccgcgaac agcgcaacca catggcgatt gttatcgacg aatacggcgg cacgtcgggt
    11161 ttggtcacct ttgaagacat catcgagcaa atcgtcggcg aaatcgaaga tgagtttgac
    11221 gaagacgaca gtgccgataa tatccacgcc gtttcttccg aacgctggcg catccacgcg
    11281 gctaccgaaa tcgaagacat caataccttc ttcggcacag aatacagcag cgaagaagcc
    11341 gataccatcg gcggcctggt cattcaagag ttagggcatc tgcccgtacg cggcgaaaaa
    11401 gtccttatcg gcggtttgca gttcaccgtc gcccgcgccg acaaccgccg cctgcacacg
    11461 ctgatggcga cccgcgtgaa gtaagcaccg ccgtttctgc acagtttagg atgacggtac
    11521 gggcgttttc tgtttcaatc cgccccatcc gccaaacata aaaatgccgt ctgaaaccgt
    11581 tgcaagtttc agacggcatt tcgcttatcc gcttattttt tgctcaaagt cagccccgtc
    11641 caaccgaata acagggtcaa agccaaactc aaatagcaga aaaaggcata cggcagatat
    11701 tcccaaaccg gcacgcccag cgcgtggctg atgaacacgc cgcatacgct ccacggtacg
    11761 agcgggttaa tcaccgtccc cgcatcttcc agcgtccgcg acagattgcg ggaatgtagt
    11821 ccgagcctgt cgtaaacggg tttaaacgtt tctccagaaa gcaggatgct caaatattgt
    11881 tcgccaatca ggaaattaac cccgaccgaa gtcatggcaa cgctgaacgt cgcgcgtccg
    11941 gcattcgtca agaaggtacg gatggcctcc aacagggaag gaatcacacc gagcgcaaac
    12001 aacagtccgc ccaaactcat cccgagaatc acgatggttt gcgtgaaaaa catactttcc
    12061 aaaccgccgc gcgaaatcag tttgacaaca tctttaaacg cttcgccttc gagtttgtag
    12121 ccgccgtaaa accacgcacc gagctgacgc agatcgggcg tgctgtgcag atacgttaca
    12181 gcaacggcaa ccatgacggt aaagagcatg gcgacgacgg cgttgatgcg catcaatgcc
    12241 aaaatgacca acagcgcaaa cggaatcagc gaatagccgt gcaccaatcc cgtggcttca
    12301 agctggctgc ggaaggattc gacgctgttc aaatcctgcg cggcgacatt cggcaaaagc
    12361 caaagcatca gtgccgcact aatgagccac gcggggatgg tggtgtacat catatttttg
    12421 atgtgctcaa acaagtcgat gccgacgatg gacgcggaaa tacccgtcgt atccgaaagc
    12481 ggggacattt tgtcgccaaa aaatgcgccc gaaacaatcg cgcccgccgt catcgccata
    12541 tcggcctgaa acgccgccgc catccccata aaggcaacgc cgacagtggc gcaggtggtc
    12601 aggctgctgc cgatggacac gccgatgacg gaacacagcg cgaaggcgga aaaataaaaa
    12661 taagtcgggg aaatcagtcc gaaaccgtaa tacatcagcg tcggaatcgc gccgctcatc
    12721 atcagcgcgc tgaccatcag cccgatgaag aaaaacaggt aaatcgcgcc cataccctga
    12781 ttcaacgcgc ctatcatgcc ctgctgcata tcgttgtatt tcaaaccgcg cgccaagccg
    12841 tacaaaatca gcacgacgat ggcggcaata atggacatat gcggcagcca ctccaatgaa
    12901 atgatggtat agcccatcgc ggcaatcaga gcgacgacga cggcaagtgc ctcaccgcgc
    12961 ggcatatcga gtaaggattt gaaagcgaac atgattcttt gcccgatttg atataaagtt
    13021 aacggattct aacacaccat accgaatccg ccgacgtttt cagacggcat tttagcgttt
    13081 tacaaaagcc ggacagaaac aaaacggcaa ttttctcaat ttaaattaat ttttttgaaa
    13141 tataattttt tatttttttg tttgttgaca tataagactt ttatggattg aagtattaaa
    13201 acctcggcac acacgccgca aacaccggta cgcaaaaatg ccgtctgaaa gatgttcaga
    13261 cgacatttct caagaaaaag attatttgtc cgccctatat ccgtacaccg caaaaaacac
    13321 gatatacaga taacagatga cggaaacgac aaacgaagac atcagggtgt aagtatctgc
    13381 cacccagccc tgcacgacag gaacgaccgc accgccgaca atcgcggtac acagtacacc
    13441 ggaagccgcg ttggtaaatt ttcccaatcc tttggttgcc aaagagaaaa tcgtcggaaa
    13501 cataatcgag ttgaaaaaac cgatggcaag cagcgaccac atcgccacat ccgcattgcc
    13561 gctacccgtc gccatcgcga cggcaagcag tacgaccgca gccgatgcgt taaacgccaa
    13621 ataacggttg ggcgcgaatt tcgccatcac cgccgaaccg aggaaacgtc cgaccatcgc
    13681 gccgccccaa tagaacgaca ggtaatgcgc ggcagaagca tgatccagcc ctttcagata
    13741 acccaatacg ttgaccatca acgaaccgat agacacctcc gcgccgacat agcagaaaat
    13801 gcctgccgta ccgaacacga gatgtttgta ttgccatacg ctggttttgc cgtcgtggtt
    13861 gtgcgcgctt tcctcggcgg caattttgcg cgcgtcgggc agccggatca ttttcacgaa
    13921 aacggcaagg ataatcagca gccccgccag tcccaaatag ggaatctgta cggaagaaat
    13981 ctgttccgcc ttgctgacgg tttgggttgc gtccgccaga atcaggaacg cgccgatttg
    14041 cggcgcaatg gtcgtaccca aagcgttaaa cgcctgaacc agcgtcagtg ttgccgattc
    14101 cttgccgggt ttcgccagca gggtaacata aggattaccg gcgacctgaa gcagcgttac
    14161 gccggaagcc aaaataaaca acgcgcccaa aaataccgcg taagaatggc tacccgcagc
    14221 aggataaaac agcaggcatc cgaccgccgt cagcagaaag ccgccgataa cgccgttttt
    14281 gtagccgact ttgccgacaa aagcccccat cgggatggac atcaccgcat aggcggtaaa
    14341 gaaacagaat tggatcagca tcgcctgaac gtaagacagg tcgaaaattt ctttcaaatg
    14401 agggataagg atgtcgttca tgcaggtaat aaaacccatc atgaagaaca gcgtggtcaa
    14461 gacgaccaat ggggacgtat gattgttttg tgattgcgca gacatgattt gcctttcgtg
    14521 gtgtcggaat attacccgtt tcaagaaaat agaaattgac atttcttaac ggcaatgcaa
    14581 atcatactga tatttgaaca ttttgcaaac tttatcggat gcaggcaatg cgccgatctg
    14641 aaattcaagc agcagaaatt aatattttac agttgcttgc atagatttta atagatttta
    14701 aaaaattccg gcggacttgc aaaaaccgtt cccaataaaa tgaaaatccg ccgccgtatc
    14761 cggaaaattc ggaaatgccg catcgtggca tcaaaaccgg agcggaaaga atgtcgtatg
    14821 cccgacaaaa aaggggcata cggctgtgcc gtattttttg caccgtcctc cgcccttcgg
    14881 cgggggcgtg aaaata
//
LOCUS       DAWGJV010000038        14776 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWGJV010000038 DAWGJV010000000
VERSION     DAWGJV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.038"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            43..675
                     /locus_tag="WHQ94_000842"
     CDS             43..675
                     /locus_tag="WHQ94_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ2128156.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WHQ94_000843"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WHQ94_000843"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ2128157.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPEIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="WHQ94_000844"
     CDS             2197..4065
                     /locus_tag="WHQ94_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ2128158.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVEFSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(4160..4773)
                     /locus_tag="WHQ94_000845"
                     /pseudo
     CDS             complement(4160..4773)
                     /locus_tag="WHQ94_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="WHQ94_000846"
     CDS             complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="WHQ94_000846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ2128159.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            7063..7287
                     /locus_tag="WHQ94_000847"
     CDS             7063..7287
                     /locus_tag="WHQ94_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128160.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPTMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            complement(7356..8657)
                     /locus_tag="WHQ94_000848"
     CDS             complement(7356..8657)
                     /locus_tag="WHQ94_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ2128161.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDNGTTYADFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSASPPAVLSDGIFLPRNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKNTSLMGAYLSAGCISPRLLARKSLERRLNAWTDNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLPAAVIHTPWRAAGSIDTHGYPAHPVAGIP"
     gene            complement(8647..9558)
                     /locus_tag="WHQ94_000849"
     CDS             complement(8647..9558)
                     /locus_tag="WHQ94_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2128162.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGVSTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9666..10064
                     /locus_tag="WHQ94_000850"
     CDS             9666..10064
                     /locus_tag="WHQ94_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ2128163.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            10113..10718
                     /locus_tag="WHQ94_000851"
     CDS             10113..10718
                     /locus_tag="WHQ94_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ2128164.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLATVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGEAGEKTFEPVAERLKVFGA"
     gene            10904..13015
                     /locus_tag="WHQ94_000852"
     CDS             10904..13015
                     /locus_tag="WHQ94_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2128165.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNLTLNGDYTIGRFENHLTVGMDYSREHRNPTLGFSSAFSASINPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTLSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLLNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            13213..13872
                     /locus_tag="WHQ94_000853"
     CDS             13213..13872
                     /locus_tag="WHQ94_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ2128166.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            14043..14738
                     /locus_tag="WHQ94_000854"
     CDS             14043..14738
                     /locus_tag="WHQ94_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2128167.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAP
                     LTEGVNYTVLSTPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEASESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         3328 a         3833 c         4102 g         3513 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcagcc
      601 tctttgttcg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaaattttg
      721 atgtcttttc cgaacaggat gccgatatga tcaaccccat cgcctcgctt tcccccctag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccggaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttctcact tgaagacgcg gccgccgtca aagccatcga agccaccacc aatcacgatg
     1021 tcaaagccat cgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagcgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gatcttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggc ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcacacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag gctttatcgg atcgctggaa atccccgtcg
     2041 aagccaaagc aaaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagttt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcgc tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgctt tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggg tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tatacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttctcgcgac ggtgcattgc agtagcctgc cttgccgtcc
     4081 gatatcgttt gtgccgccgt ttgcctttcg gggcggcggc ggtttttatt ttccttcccc
     4141 tgcgggaggg aattttgaat caaaacccca attgcctttc caagttttcc accagattgt
     4201 catccagttc caaagcctgc gacaggcggg cgaggaagac ggtttctttc cgcgacaaat
     4261 cggcacatac cagtcttgcc gccagatagg cctccgccgc caacgcctca tcgttgccga
     4321 cggcggcggc gatgtcttcg atgcttgcgg gcaggcggta ttcggcggcg agccatgcag
     4381 cagtttcggg gtctgtgccg ctttcctgtt cgatagtccg gcgttcggct tcgtctatca
     4441 tgccgtctga agcggcggtg gctatcatgg tgcgcaatac ggtgcggctg tatgtttctt
     4501 cggtttctcc ggcaggttgg aaatcgcttt gtgttacggt tgcccgccct ttgttttgct
     4561 gccacatctg atagccccgg taggcgaggt agcccaaagc ggcggtcgaa ccgattttgg
     4621 tgatggtttt gcggttttta ccgttcagca gcatggaggc gacaccggca accagcgcgc
     4681 ctccgccgaa tgaattgagc gggctgtcgg agaatgtgtt gccttttttt gaaccgtgct
     4741 taagacttgg ttgagcagtc gggtaaagtt catgaatttt tcctttctgt tgcgggaagg
     4801 gcggtatgtt taccctatcc ttttaaacgg cggcaggccg gccaataatt gttggccgta
     4861 ccgctgtgtt ttgatgcggt tgtcgaggat ggttacgcgg ccgtagtctt gttcggtgcg
     4921 gatgaggcgg ccgacggcct ggatgagttt gatgccggct tcggggacgg tgatttcaat
     4981 aaaaggattg ccgccgcgct gttctatcca gcggttttgg gttttttcga tggggttgtc
     5041 aggcatcgca aacggcagct tggcgatgat gacttgcacg caggcggtgc cgggcaggtc
     5101 gagtccttcg gcgaaactgt cgagtccgaa gatgatgctg gctttgcctt cttctatggc
     5161 ccggtggtgt ttttgcagga ggacggcttt gggtaattcg ccttgtacga gcaagagcgg
     5221 caggtagtct ccgggcaggc gcagggcgac atcctgcatt tgtttgcgcg aggaaaacaa
     5281 gacgagcgtg ccgatggctt cggtgggcga aataagcttg ggcagccatt cgatgacggc
     5341 ggcggtgtgg gcttcggggt ctttggggct ggcgtatatg ggggggatgt agagttcgcc
     5401 ctgtttttca aagtcaaagg ggcttttgag ggcgagggtg gtggtttcgg gcagccattg
     5461 cagcccggtt tggcgcagca tcaggttgaa gttgcccaag gattgcaggg tggcggaagt
     5521 caataccgcg cctgccgcac gccgccacag gctgttggca aggtgggatg cgctgctgat
     5581 ggggctggcg ttgaaaatgt agtcgttttt gtcgtcggca cggcgggtta tccattttgc
     5641 cagcggctct tcgccttcga gggggacggt ggagagcaaa tcccaaaccg cgctgatttg
     5701 ttcgatacgg acgatgaaga tgccgaactc gccggtcagg cggtcgagga gcgcgccgtc
     5761 ctgttctttt tcgcggcgcg cggcggaaag cgcatcgttc agcccgacaa cgtgtttgag
     5821 caggctgcgc gcagcaatgg ccgtattgga aacggtggtt tcgaggcctt cggggatttt
     5881 gccgtcttcc cacagccaag tcggttcgct gttggttcgt ctgtcgtttt cagacggccc
     5941 catacttaaa gacggctctt ccgccaaatg gaactgccat tcgtgcaggc tgtcgagcaa
     6001 ggatgcggcg gcttcgtcgg ccaggttggc aagttcggct ttatcggtaa gcgcggcaat
     6061 tttgccggtc agctgcggca gtttttccag cgtccaaacg gcaatattcc atgaatgttc
     6121 ggcggcaaaa cggctgaggg cttttttggg caggtggtgc gcttcgtcta tgcaatagaa
     6181 actgttttcg ggcgcaggca gaatcacgcc gccgcccata ctgatgtcgg caagcagaag
     6241 atcgtggttg gcaacgacga catcgacggt ttccaagaca tcgcgtgcta ggtaaaacgg
     6301 acattccgga cggttgggac aggcggtttt caggcagccg tggcggtcgt tggtcacttt
     6361 gagccaaatc gcgtcatcga ttttttccgg ccaagtgtcg cggtcgccgt tgaaccgtcg
     6421 ggcggaaaat tcgtcggcga tgtcgcgcag cagcttcaat tcttcgggct tgggtttgct
     6481 gtcccacaag acggcggggg cttcaaagcc gagcaggttt tgctgggcat tgctttgcgt
     6541 cagttgatag agtttgtagg ggcagagata gcggccgcgc cctttggcaa gcgcgaagct
     6601 cagttccaaa ccgctttttt cgaccaaaaa cggcaggtcg cggtctacca actgctcctg
     6661 caaggcaacc gttgcgctgc tcacaatcag ccgcttgccg cgcgtctgcg ccatgatgcc
     6721 gcccgccagc aggtaggcaa gcgatttgcc cacgccggtc ggcccttcga ttacggcgat
     6781 gctctcgccc tcgcgcttgg gcggctcgcc gccttcttcg cgcgccaacg tccgcgaaaa
     6841 agcgttggca accgccgcaa tcatttcccg ctgcgaagcg cgcggacgga aaccgggcag
     6901 gtttttgccg atgttttggt aatggtcgcg gatggcgttt ttttctaaat cggtgagcat
     6961 ggcgttttgt acggcggtag aagtgggctt attttaacat tgcacggaag cggtacaata
     7021 tcgttgtcgg aatggggggt gaggtgaatc gtgcggacgt ggttggtttt ttggttgcag
     7081 cgtttgaaat acccgttgtt gctttggatt gcggatatgt tgctgtaccg gttgttgggc
     7141 ggcgcggaaa tcgaatgcgg ccgttgccct gtgccgccga tgacggattg gcagcatttt
     7201 ttgccgacga tgggaacggt gtcggcttgg gtggcggtga tttgggcata tctgattatt
     7261 gaaagtgaaa aaaacggaag atattgagta gtccgtataa aaatgccgtc tgaaacggaa
     7321 gttcagacgg catttgtttt aggttgccgt gccgcttagg gaataccggc gacaggatgg
     7381 gcgggatagc cgtgggtgtc gatgctgccg gcggcgcgcc acggggtatg gatgactgcc
     7441 gccggcaggt gggcaagctc ggggatgtgc cgtctgacaa aggtgccgtc ggggtcggtc
     7501 ttgtgtgcgg cggcggcaat gtcggggcag gtgtgccgtg aggcggcaag ccgccagttg
     7561 ccttggttga ttgccgcatc gaaatcggtc agctgccggg caaaccatat ctcgccttcg
     7621 cggcggggga ggtttaaaac gtggcagaaa aaatccgcgc tcaagcgtct cagggcgggg
     7681 tggaggctgc cggttttgtg caaacagcgc atcgcggcat cgataatcgg aatgccggtc
     7741 cggccctgct gccaaagcgt caggcgcagg gtgtgttcag gattgccgtc tgaagggtcg
     7801 tcatccgtgt gctgcaaggc aagttgaagg aaaaaatcgc ggcggatgat gttgtccgtc
     7861 cacgcgttca gacggcgttc gaggcttttc cgcgcgagca ggcgcggcga gatgcagccg
     7921 gcactcaaat acgcgcccat cagcgaagtg tttttgcgcg aggggaaatc ctttaagacg
     7981 gagtaggaat ccgcctgttc gagaaaccgc cgccactgcc gccaagccgc cgtttcaccg
     8041 ccgtttcgcg gcaggaagat gccgtctgaa agcacggcag gcggcgaggc ggaaaggttt
     8101 tcggggaagg gttggcggta tgccgcgaat aggtccggac cggcgggggg ctgcttggaa
     8161 aagcggtcga gccatacttc gcggtagcgg tcgaaatcgg catatgtcgt gccgttgtcg
     8221 ggtatcaggt cggttttgcc gaaaacggcg cggtcattga cgaaggttaa cgcgatgccg
     8281 tgtttgtcca attcatgcca aagggcgttg tcggcgagtt tgtcggcaaa agtatgggat
     8341 tcgtcggcga tgacggcgcg gatattgagg cggacggcga gccggacgag ctcggcaggc
     8401 gatgccgccg tgtagagcgg gatgccgcgc cctgcaagcc cttgggcgag ttcggcggcg
     8461 gattggcggt agaacgcggc gcggcgaggg ttgtctgttt cggcatcgtc aatccaaata
     8521 ccgataacgg gcaaaccttc ggcaacggcg gcgcataagg cggcgttgtc gcggatgcgg
     8581 aggttttggc ggaaccagac gagcgtgtgt gcggcgcgcg tgtccgcata aagggggcgg
     8641 gcggtttcag acggcatttc ggcagccttt cctgctggcg attttttcgt tcagaaaatc
     8701 gatgaagctg cggactttcg cgcttaagaa tgccctgtcc gcataaacgg cgttcagccg
     8761 gtcggtcggg acggcgtatc cgggcagcag cctcaccagc gtgccgcagc gcaaatcgtg
     8821 ttccgccgcc caaagcggct gataaccgat gcacgcgccc gccttaatca tttcgcgcat
     8881 catcagcgtg ttgtcggtac ggatgacggg ggtcagttca agccggtatt ttttgccgtc
     8941 cgatttgcgg gtgaggtcga gtttctgctg gttggtgtag gtcggcagga cggcgggcag
     9001 ccccgccact tcttccggcg tttccggcac gccgttgcgc ctcaggaaat cgggcgaggc
     9061 gagcagggca aattcgattt ccgccagcgg gcgcgcaatc agcgacgggg acagggtttg
     9121 ggaaacgcgc aacgccaaat ccacgccttc ggcaatcaaa tcgacgtggc ggttgtccaa
     9181 aatcagttct aatgttactt cgggataacg ttcgcggtat tccgccagcc agttgcatat
     9241 ctggctgccg gcaaaccaca gtggcatcgt tacgcgcagc agcccctgcg gtttttccgt
     9301 ccccccggcg gctttttgcg cggcatcgtc gagcgtgtcg agcgcgtaac tgcattgccg
     9361 gtagtattct tccccggctt cggtcaggct gaggttgcgg ctgttgcggt gcaggagttt
     9421 ggcttggacg gtgttttcca agtggctgac gtgtttgctt gccattgcgg tggagacgcc
     9481 gagcgcgtcg gcggcgcggg tgaagccgcc gctttggacg acttggcgga aaaccttgag
     9541 gctgaacagg gtgtccatat tttcttgtgt ggaaaagttg tatcaataaa agcagtatat
     9601 atttgaaaag gggaaacatc tatactctac cgcctgaaat gaagacaaat atcaaaggag
     9661 cttttatgtc cgattgctgc aaccgtatcc aaccggtttt gctttctgtt ttgcgtatcg
     9721 taaccgccta cctgtttttg ttgcacggta cgtcgaaaat cttcgccttc cccattgaaa
     9781 tgggcagcgg ttcgcccggc gggctgttgc tgcttgccgg tattttagaa attgtcggcg
     9841 gcattttgct ggtgttgggc ctgtttgcgc gccctgccgc gtttgttttg tccggtcaga
     9901 tggcggttgc ctattttatg gcgcacgctt ccggaaatgc tttgttcccg attgccaacg
     9961 gcggcgagtc cgcagtgctg ttctgcttcg tattcctcta tatcgcggca gcgggcggcg
    10021 gagcatggtc gctggacagg ctgtttttca agcgtaaagc ctgaatcgga ctgcctaaag
    10081 tgtattttgt tgaatgtttt tgaggaaaag aaatgacccg tcaatctctg caacaggctg
    10141 ccgaaagccg ccgttccatt tattcgttaa ataaaaatct gcccgtcggc aaagatgaag
    10201 ttgtccaaat cgtcgaacac gccgttttgc acacaccttc ttcgttcaat tcccaatctg
    10261 cccgcgtggt cgtgctgttt ggcgaagagc atgataaggt gtggcaattt gtcgaagacg
    10321 cgctgcgtgc cgtcgtgcct gccgacagtt ttgaaccgac cgcgcaaaaa ttgaacctgt
    10381 ttaaggcggg tgcggcaacc attttgtttt atgaagatca aaatgtcgtc aaaggtttgc
    10441 aggagcagtt ccctgcttat gccgccaact ttcccgtttg ggcggaccag gcgaacgcga
    10501 tggtacagta tgccgtctgg acgacacttg ccacggtcgg cgtaggtgca aacctgcaac
    10561 attacaaccc cttgcccgat gcggcgattg ccaaagcgtg gaatattccc gaaaactggc
    10621 tgttgcgcgc gcaaatggtt atcggcggta ttgaagggga ggcaggtgaa aagacctttg
    10681 aacccgttgc agaacgtttg aaagtgttcg gcgcataatt tcgcggtcaa aaaaatgccg
    10741 tctgaaccct gttcagacgg catttttcag tatcaggcgg cgagttttcc gcattctgag
    10801 acctttgttt acaaatatca tgttcaatat agttaaaaga aattattctc atttcctccg
    10861 tgaggcaata taattcggtt gttttgttaa attgagtata aaaatgaaaa tatcatttca
    10921 tttagcttta ttacccacgc tgattattgc ttccttccct gttgctgccg ccgatacgca
    10981 ggacaatggt gaacattaca ccgccactct gcccaccgtt tccgtggtcg gacagtccga
    11041 caccagcgta ctcaaaggct acatcaacta cgacgaagcc gccgttaccc gcaacggaca
    11101 gctcatcaaa gaaacgccgc aaaccatcga tacgctcaat atccagaaaa acaaaaatta
    11161 cggtacgaac gatttgagtt ccattctcga aggcaatgcc ggcatcgacg ctgcctacga
    11221 tatgcgcggt gaaagcattt tcctgcgcgg ttttcaagcc gacgcatccg atatttaccg
    11281 cgacggcgtg cgcgaaagcg gacaagtgcg ccgcagtact gccaacatcg agcgcgtgga
    11341 aatcctgaaa ggcccgtctt ccgtgcttta cggccgcacc aacggcggcg gcgtcatcaa
    11401 catggtcagc aaatacgcca acttcaaaca aagccgcaac atcggtgcgg tttacggttc
    11461 gtgggcaaac cgcagcctga atatggacat taacgaagtg ctgaacaaaa acgtcgccat
    11521 ccgtctcacc ggcgaagtcg ggcgcgccaa ttcgttccgc agcggcatag acagcaaaaa
    11581 tgtcatggtt tcgcccagta ttaccgtcaa actcgacaac ggcttgaagt ggacggggca
    11641 atacacctac gacaatgtgg agcgcacgcc cgaccgcagc ccgaccaagt ccgtgtacga
    11701 ccgcttcgga ctgccttacc gcatggggtt cgcccaccgg aacgattttg tcaaagacaa
    11761 gctgcaagtt tggcgttccg accttgaata cgccttcaac gacaaatggc gtgcccaatg
    11821 gcagctcgcc caccgcacgg cggcgcagga ttttgatcat ttctatgcag gcagcgaaaa
    11881 tggcaactta atcaaacgta actacgcctg gcagcagacc gacaacaaaa ccctgtcgtc
    11941 caacttaacg ctcaacggcg actacaccat cggccgtttt gaaaaccacc tgaccgtagg
    12001 catggattac agccgcgaac accgcaaccc gacattgggt ttcagcagcg ccttttccgc
    12061 ctccatcaac ccctacgacc gcgcaagctg gccggcttcg ggcagattgc agcctattct
    12121 gacccaaaac cgccacaaag ccgactccta cggcatcttt gtgcaaaaca tcttctccgc
    12181 cacgcccgat ttgaaattcg tcctcggcgg ccgttacgac aaatacacct ttaattccga
    12241 aaacaaactc accggcagca gccgccaata cagcggacac tcgttcagcc ccaacatcgg
    12301 cgcagtgtgg aacatcaatc ccgtccacac actttacgcc tcgtataaca aaggcttcgc
    12361 gccttatggc ggacgcggcg gctatttgag catcgatacg ttgtcttccg ccgtgttcaa
    12421 cgccgacccc gagtacaccc gccaatacga aaccggtgtg aaaagcagtt ggctggacga
    12481 ccgcctcagc actacgttgt ctgcctacca aatcgaacgc ttcaatatcc gctaccgccc
    12541 cgatccaaaa aacaaccctt atatttatgc ggttagcggc aaacaccgtt cgcgcggcgt
    12601 ggaattgtcc gccatcgggc aaatcatccc taaaaaactc tatctgcgcg gttcgttggg
    12661 cgtgatgcag gcgaaagtcg ttgaagacaa agaaaatccc gaccgagtgg gcatccattt
    12721 gaataacacc agcaacgtta ccggcaacct gtttttccgt tataccccga ccgaaaacct
    12781 ctacggcgaa atcggcgtaa ccggtacagg caaacgctac ggttacaact caagaaataa
    12841 agaagtgact acgcttccag gctttgcccg agttgatgcc atgctcggct ggaaccataa
    12901 aaatgttaac gttacctttg ccgctgccaa tctgctcaat caaaaatatt ggcgttcgga
    12961 ctctatgccg ggtaatccgc gcggctatac tgcccgggta aattaccgtt tctgatgaaa
    13021 tcaggcaaag gccgtctgaa aatcctgata atccgtaaat tcagcatacg aactttttga
    13081 caggctttat ctacatcagt ataatccaaa aagaaatagt gcccttggca gtacgcatta
    13141 cccattaaat catagcgggt atgccgatcc caagggcttt tttatatctt tgacaaatat
    13201 aggggaagca atatgaaaac cgccattttg attgacggtg cattttttat taagcgggta
    13261 cggtcattag gcctgtctca aaatccacac gatgcaaaaa gaattgctga tttggctttc
    13321 cgatattcga tgctgcatct gaaagataaa atcaacggca cagatcaaat ggatgattta
    13381 tatagaattt tcttttacga ctgcgtgccg tttgaaaaga aaatgcacaa tcccgtatct
    13441 catcgtgcca tagatttttc aaagactccg gaagccatat ttcgtcgcaa tctgcatacc
    13501 gaattgaaga agaagcgtaa attagcgtta cgtttaggca agctgtcgga caatacagca
    13561 tggatattaa aaccccaagt catgaaaaat cttctgaaaa acccgtcaac tcaaattacg
    13621 gaaaacgatg tcgtgctcga tgttaaacaa aaaggtgtag atatgcgtat aggcttggat
    13681 atttcatcta ttaccttaaa aaaacaagcc gataaaatca tcttgttttc tggtgattcc
    13741 gattttgtcc cagcagccaa attagccaga cgggaaggta tcgattttat tcttgatcct
    13801 atgtggaatc atgtcccacc tgacttaatg gaacacatcg acggattgaa gacaaaatgt
    13861 ccgaaaccgt aaattactag aacaaaggcc gtctgaaaat aaggctaatt atctaagcca
    13921 tatttcagac ggcctttata aaggctgaaa taaaactaaa cacatttttt cactcaaatc
    13981 gaacacgcct tcaataaaat gtcataaaat ccgcacatta atctgacaca caagagatac
    14041 ctatgaaact gaaaacctta gctttgactt cattgaccct gttggcattg gccgcttgta
    14101 gcaaacaggc tgaaaccagc gttccggcag acagcgtcca aagcagctca tctgctccgg
    14161 cagccccagc cccattgacc gaaggcgtga actacactgt attgtccacg cctatcccgc
    14221 aacagcaggc cggcaaagtc gaagtcttgg aatttttcgg ctacttctgc ccgcattgcg
    14281 cccatcttga gccggtcttg agcgagcaca tcaaaacgtt taaagacgat acctatatgc
    14341 gccgggagca tgtcgtgtgg ggtgatgaaa tgaaaccttt ggcacgtttg gcggccgcag
    14401 tggaaatggc cggtgaatca gataaagcca acagccatat tttcgatgcg atggttaatc
    14461 aaaaaatcaa tctggccgat accgataccc tgaaaaaatg gctgtccgag caaacagcgt
    14521 ttgacggcaa aaaagtattg gctgcatttg aggcttctga aagccaagcg cgtgcggctc
    14581 aaatggaaga gttgaccaat aaattccaaa tcagcggcac accgactgtg atcgtcggcg
    14641 gcaaatacca agttgaattt aaagactggc agtccggtat gaccacgatt gaccagttgg
    14701 tggataaagt acgcgaagag cagaaaaagc cgcaataagt tgaggattga atgagtaaag
    14761 gccgtttgaa aatagg
//
LOCUS       DAWGJV010000039        14475 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWGJV010000039 DAWGJV010000000
VERSION     DAWGJV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.039"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            167..655
                     /locus_tag="WHQ94_000855"
     CDS             167..655
                     /locus_tag="WHQ94_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128168.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNSQPASQPASQPASQPASQPANYT
                     LN"
     gene            1702..2346
                     /locus_tag="WHQ94_000856"
                     /pseudo
     CDS             1702..2346
                     /locus_tag="WHQ94_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2336..5116
                     /locus_tag="WHQ94_000857"
     CDS             2336..5116
                     /locus_tag="WHQ94_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ2128169.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKYRLPKFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQE
                     KGVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIF
                     TVDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAF
                     EGKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVL
                     ATFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQE
                     TQFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSK
                     LNIQNRNELQNKLLKDWQIEFLGLGQDKQVCPDDKLAGCLKILEMVEKHLNESDKPFI
                     GTKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISA
                     RLGDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQI
                     FIEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFT
                     ESFPLGAASLEK"
     gene            complement(5265..6437)
                     /locus_tag="WHQ94_000858"
     CDS             complement(5265..6437)
                     /locus_tag="WHQ94_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ2128170.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVPAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6787..7233
                     /gene="iscR"
                     /locus_tag="WHQ94_000859"
     CDS             6787..7233
                     /gene="iscR"
                     /locus_tag="WHQ94_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ2128171.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7262..8476
                     /locus_tag="WHQ94_000860"
     CDS             7262..8476
                     /locus_tag="WHQ94_000860"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ2128172.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            8622..9467
                     /locus_tag="WHQ94_000861"
     CDS             8622..9467
                     /locus_tag="WHQ94_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEZ2128173.1"
                     /translation="MKIANALIHNTVRIECLSADGQSISTGTAFLFLFDFDKTGVIPV
                     LVTNKHVVFVESAKKIAITLTKDENGSPNHKENITFTIEDFIQNCLPHPDENIDLCIA
                     PVGHFFNQLINHNFSPFIKGIRESDIMQTEEMDQLSAFEEVFMIGYPNGLWDSKNNLP
                     IFRTGITATHPAIDFEGKKEFMIDVACFTGSSGSPVFKYIPAGLRKIENDGFAITADE
                     TFKLMGILYAGPQQTISGEIYITTVNKPISVSSIPINLGFVIKAEKLLDFKPILKNFL
                     EKNRE"
     gene            9810..10196
                     /gene="iscU"
                     /locus_tag="WHQ94_000862"
     CDS             9810..10196
                     /gene="iscU"
                     /locus_tag="WHQ94_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ2128174.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            10283..10603
                     /gene="iscA"
                     /locus_tag="WHQ94_000863"
     CDS             10283..10603
                     /gene="iscA"
                     /locus_tag="WHQ94_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ2128175.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(10563..10784)
                     /locus_tag="WHQ94_000864"
     CDS             complement(10563..10784)
                     /locus_tag="WHQ94_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ2128176.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            10866..11366
                     /gene="hscB"
                     /locus_tag="WHQ94_000865"
     CDS             10866..11366
                     /gene="hscB"
                     /locus_tag="WHQ94_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ2128177.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            11464..14214
                     /gene="gyrA"
                     /locus_tag="WHQ94_000866"
     CDS             11464..14214
                     /gene="gyrA"
                     /locus_tag="WHQ94_000866"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ2128178.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            14371..14475
                     /locus_tag="WHQ94_000867"
                     /pseudo
     CDS             14371..14475
                     /locus_tag="WHQ94_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         4476 a         3491 c         3111 g         3397 t
ORIGIN      
        1 gcaaaatcac gacacactcg gacacccgcc ccgcttatcg caactttgcg aacgccccgg
       61 aaacagcaaa tacagcaaat aattgatttt attagagttt atttgcaaaa ccatttgccg
      121 ttacacaaga atggcacatt aaaatgactg atgaggattt ataacgatga agacagacat
      181 tcaaaccgaa ttaacccaag ccctactacc acacgaaaaa gtatgggcga acgaagaaaa
      241 aaccatttta gccaaaaaca ttctgttgga tttggtggaa aaaaccgacc cgaccattat
      301 cggtttgtta ttgaataatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt
      361 tttggtgttt aaattgcagg atttacgttt tttcttggac aaacacagcg tcaataattc
      421 ctacacaaaa tacgccaacc gcattggttt gacggacggc aaccgctttt tgaaagacaa
      481 ttcggatatt gtgttggatt ttccgtttaa agattgcgta ttgaatggcg gacaaagcac
      541 cgaagaaggc gaagagattt attttaaacg cagtaatagc cagccagcca gccagccagc
      601 cagccagcca gccagccagc cagccagcca gccagccaat tatacactaa attaacccga
      661 aaaagacaag aaatcttttt taatcaaacg cttgcttttg atgaaatcga tcggcttttt
      721 gacgcaaaag cattctcaaa attctctcgc tataccgcag acggcaaaca agcggttggc
      781 gaaatcaaac gtcattcaga cggcacaccc gccgaaaatc tcattatcaa aggcaataat
      841 ctgatagccc tacattcgct tgccaaacag tttaaaggca aagtgaagtt gatttatatt
      901 gatccgcctt attattttgt taaaaagaaa caacaagata gttttggtta taacactaac
      961 tttaaattat ctacttggct tacatttatg aaaaatcgtt tatcaattgc caaagaatta
     1021 ttaactgatg atgggattat tgttatttcc attgatgatg acggaaatgc ttatttaaag
     1081 attttgttag atgaaatttt tggatttgaa aattttatcg gaaatttacc cactattatg
     1141 aatttaaaag gtaataatga tgaatatgct tttgcaggca ctcacgaata cacactagtc
     1201 tttgctaaaa ataaagataa atctactttt tatgaatttc caatagatga agataatttt
     1261 ttagagaaat gggaagaaga tgaaattggg ttttataaaa aaggagctcc aatgcgagca
     1321 acaggaaccg aagataaaag agaagataga cctgaaatgt tctatccttt tttggtaaaa
     1381 aataacacag ttagtacaat tacagatgaa gaattttctc aaatctataa taaagattta
     1441 gaagtattca atgatgattt tattcaaaaa ctaaaagaga aatacgaaaa tcttggatac
     1501 aattttattt tacctattgc tgataaacaa tgggggcgtt ggcgttgggg gtattcaata
     1561 aagaataagg caaggctgca gacggatatt attgttagcc aatctaaaaa tggaatttcc
     1621 ttgtataaaa aacaaagacc tgaattagat gatttaccta caaaaaaacc taaaactatt
     1681 ttttataaac ctgaatacag tagtggtaat ggaactgaac agatgaaaaa tctttttggt
     1741 gaaaaggcat ttaaaaatcc taaaccagaa gaacttatcc aagattttat taccatcaca
     1801 accaacgaat ccgacatcgt cttagactac catcttggta gtggcacaac cgccgccgtt
     1861 gcccacaaaa tgaaccgaca atatatcggt attgaacaaa tggattatat tgaaacgctt
     1921 gccgttgaac gcttgaaaaa agtgattgat ggcgagcaag gcggtatttc caaagccgtg
     1981 aattggcaag gtggtggcga gtttgtttat gccgaacttg ccccatttaa cgaaaccgca
     2041 aaacaacaaa ttttggcttg cgaagattca gacggcatca aaacgctgtt tgaaggttta
     2101 tgcgaacgct atttcttgaa atacaacgtc agcgtaaatg aatttagtca aatcattcaa
     2161 gagcctgaat ttcaatcttt gccattagac gaacaaaaac aaatggtgct tgaaatgtta
     2221 gatttaaatc aaatgtatgt ttcattatcc gaaatggacg acgagcaatt tgcagattgc
     2281 ctgaacgatg acgataaagc ggtaagccgt gcgttctatc aagcggagaa aaaaaatggc
     2341 gaataataaa actttgtttg aagtaattga aaaccgcaaa gctgtgtatt tggaagacgg
     2401 agatgatgaa aaatacaggc tgcctaaatt tgtttcaaat aatttgaaat atccgttttt
     2461 tgaatggcaa aaatcggctt tagaaaactt tgtgattttt gaccgcactt caaaattaaa
     2521 ggattttccc gatatcaaaa accgccctac tcatttgcta tttaatatgg caacaggtgc
     2581 aggcaaaacg atgatgatgg cggcattgat tttgtattat tttgaaaaag ggtatcggca
     2641 ttttctgttt tttgtgaatc aaaacaatat cgtggataaa acggaaaata attttattga
     2701 cccgacacac gcaaaatttt tatttaccga gaagattttg cagggcgata cggtaattcc
     2761 cattcgcaaa gtggaaacgt tcagcccaca ttcagacggc attgaaatta aatttaccag
     2821 cattcaaaag ctgtataacg atattcacac cgaacgggaa aaccaaacca cattggcgga
     2881 tttgcacgaa ttgaatcttg tgatgctggg cgatgaagca caccatttaa acgcgcaaac
     2941 caaaggcaaa aaacaaggcg agttggattt ggaagtggaa ttaaaagcca atgcaggcaa
     3001 tgccgaaatt gaacgcaaag gctgggagca tatggttttg gaattgttac tcaataaaaa
     3061 tggcaatccc agccaaaatg tgctgttgga atttaccgcc acgctgcctg aaaatgctga
     3121 cgtgcaacaa aaatacgcgg ataaaatcat cacaaaattt ggcttaaaag aatttttgca
     3181 aaaaggttat accaaagaaa tcaatttggt atccagtacg ctgggcaaga aagagcgggt
     3241 gttgcacgct ttattatttg cttggtatcg ccatcaaatc gctttgaaat atggcattgc
     3301 caatttcaag cctgtgatgt tgtttagaag taagacgatt gatgaatcaa aagcggatta
     3361 tctggcattt ttaaattggg cagaaaatgt gcaggcggtt gatttttcgt ttttaactac
     3421 attttcaaca agcttgaacg atagcgatag cgataacgcc aacgaacaag gcaaaacccg
     3481 cactgaacaa gccctaaaat ttatgcagga aaaaggcgtt gagtttgcac atttggcaga
     3541 ttgggtaaaa caaaattatc aaaaacacaa tgtgattatt accaactccg aaaccaacaa
     3601 aaccaaaacc gaaaaaaccg acagcgaaac agaaaaattg ctgaataatt tggaagcggc
     3661 tgataatccg attcgtgcca tttttacggt ggacagatta accgaaggtt gggacgttct
     3721 gaatttgttt gatattgtgc gtttgtatga agggcaaaac ggcggcggtt caaataaaaa
     3781 atcaggcaaa acggctgccg ccaccgtatc ggaaaagcag ttgattggtc gtggcgtgcg
     3841 ttattttcca tttgcgtttg aaggtaaaca gccgaataaa cgcaaatttg acaacgatat
     3901 gcaacacgaa ttgcgtatct tggaagaatt gttttattac acgcacgatg agcaatcccg
     3961 ctatatttca gaactgaaaa acgagttgcg aaaagacggt tatttgcctg aaaaagacga
     4021 tgataaggta ttggcaacat ttaaactcaa atctgaattt gccgctaata aggattttag
     4081 agaattgtta atttgggcga ataaaaaaat ccccaaccca aacgccaaaa ccaataatgc
     4141 aaacagcctg caagccaatc cgcaaacgct ttcattccaa gttcacggca atcaactgtt
     4201 gcaggaaacg caatttacag ccgatgaaaa tgatgaaaaa gtcaagcaac ttggcacgca
     4261 aaataatttt actcaaacca taaaaatgag tgaaatggag cggcacattt tcaataaagc
     4321 cctgcatatc aaagggaaaa atagtcaatc tttattccat tttgaccgcc tgcaaagcaa
     4381 actcaatatt caaaatcgca atgaattgca aaataaattg ttgaaagact ggcaaattga
     4441 atttttagga ttagggcaag acaaacaggt ttgcccggac gataaacttg caggctgcct
     4501 gaaaatcttg gaaatggttg aaaagcattt gaatgaaagt gataagccat ttatcggtac
     4561 aaaagaattt acgcctaaaa aattatggga aattttcggc acgccaaaac aaaaatgggt
     4621 caaaaaagat gatgtaaaaa cgactattgc cacgcaaaat gattggtatg tgatgaataa
     4681 ttttgccgga acgggtttgg aagaagcgtt aattcaattt atttcagcgc gtttgggcga
     4741 tttgaaatct caatatgaca ttcatttaat ccgtaacgaa gaagtgttta aattgaataa
     4801 ctttgccaat ggcgaaggat ttatgcccga ctttatttta ttgctgaaag ataaacaaaa
     4861 atcttcttcc aatggcgtga atgacttttt gcattaccaa attttcattg agcccaaagg
     4921 tgagcattta gtggaaactg atggatggaa agaagaattt ttagaagcca tcacagtgga
     4981 atatgggatg gataagatcc tgcaaaaaga tacaccgcat tatcgtttga tcggtttacc
     5041 gttttttact gatcatcaga aaaatggaca atttacggag tcattccctt taggtgcggc
     5101 atcgcttgaa aaatagagta gtgcattgca ggcaaccccg tttgacaaaa cttcctttac
     5161 aaaagggcgt tttgtcagat atttaatcaa cacattatta aaatacagcc aaattttaat
     5221 gccgtccgaa ccctgtgttc agacggcatc gtatttttca gtatctaaac cgtttcccta
     5281 ccccaatctt taccccttaa aatcgaggca tcgacatctt gaatatcgcg gtgtcccgta
     5341 aacgccatag atatatccat ttctttatat agaatttcca gcgcacgggt tacgccttct
     5401 tcgccatacg cgcccaagcc atacaggaac gcccgaccta tcattgtacc tttcgcgccc
     5461 aaagcccacg ccttcaaaat atcctgaccg ctgcggatgc cgctgtccat ccaaacttcg
     5521 atgtcgctgc ccactgcgct gacgatgtcg ggcaaggctt tgatggcagg cacggtatcg
     5581 tcgagctgtc ggccgccgtg gttggaaaca atcaatgcgt ccgcgccgct tttcgctgct
     5641 ttttccgcgt cttcgggttc cataatgcct ttgataatca gcttgccgcc ccacaaatct
     5701 ttaatgcgcg ccacatcgtc ccagctcagg cgcgggtcga attgttcgga agtccatgaa
     5761 gacagcgaag acaaatcgcc gacgttcttc gcgtgtccga cgatattgcg gaacgtgcgg
     5821 cgttccgtgt tcagcatttt catgcaccat tccggtttgg tcgccagatt gattaaattg
     5881 gcgatggtcg gtttcggcgg cgcggacagg ccgtttttga tgtctttgtg gcgttgaccc
     5941 aaaacctgca aatcggcggt caataccaat gccgaacatt tggcatcctt cgcgcgctta
     6001 atcaggtttt ccataaactc gcggtcgcgc atcacataaa gctgaaacca aaacggcgcg
     6061 ctggtgttct cggcaacgtc ttcaatcgag cagatggaca tggtggacaa cgtaaacgga
     6121 ataccgaact tctccgccgc ccgcgccgcc aaaatttcgc cgtctgcgtg tgccatgccg
     6181 gtaaaacccg tcggcgcaat cgccaccggc attttcacat cctgcccgat cattttagtc
     6241 tcaaggctgc gtccctccat attgaccaat actttttgac ggaagcggat gtctttgaaa
     6301 tccgaagtat tttcccgata agtcgtttcc gtccacgaac ccgaatcgat gtaatcgtaa
     6361 aacatacgcg gcattttgcg cttggcaacg cggcgcaagt cttcgatgca ggtcattttg
     6421 ctcaaatcac gtttcatctt gtttttccct gtttgacagc ctgatggaat atgccgtccg
     6481 aaaccgcttg agccttcaaa cggcatttca ggcaataccc aaataaatca ctaagattta
     6541 gataatctat atcagtatca aaaataaatc aaatcgtttt gaaaccgctt gccggcaaaa
     6601 taccccgacc gccgctcctc aaaaaacata accaactgat ttaaaaaacc aaacaaataa
     6661 agcaaaatca gactataccg ccatttaaac aaacaatcgg caacagctcc cccttacttg
     6721 actgaaacac tcagatattg gacaattctc cccactataa aaaagccgac acgggcaacc
     6781 accaccatga gactgaccac caaagggcgt ttcgccgtta ccgctatgct ggatttggcg
     6841 atgaacgcgc aaaccggcgc cgtcaaactc agtgccatca gcgaacgcca aaacatatcc
     6901 ctctcctatc tcgagcaatt gttcggcaaa ctccgccgcg ccggactggt tgaaagcctg
     6961 cgcgggcccg gcggcggcta catcctcgcc gcaccggcgg cacgcatcaa catcgcccaa
     7021 atcatcgccg ccgccgaaga ccggctggac gcgacccaat gcggcagcaa agccaactgc
     7081 caccacggcg cgccctgcct gacgcacgac ctttgggaga atttaaacaa aaccatcaac
     7141 gactacctcg gcagcgttac cctgcaaagc atcatcgaac agaaaaacaa cggcgacggc
     7201 agccgcgtcg tccaatttac acacatccat taaataacac ccgaaaaaga aagagcaaac
     7261 catgaccgtc aaaacccccg tttacctcga ctacgccgcc accacccccg ttgacaaacg
     7321 cgttgccgaa aaaatgattc cctatctgac cgaaaccttc ggcaaccccg cctccaacag
     7381 ccacgcattc ggctgggaag cagaagaggc cgtcgaaaaa gcccgcgccg acatcgctgc
     7441 cctgattaat gccgacccca aagaaatcgt cttcaccagc ggcgcgaccg agtccgacaa
     7501 cctcgccatc aaaggcgcgg caaacttcta caaaaccaaa ggcaaacacc tcattaccgt
     7561 caaaaccgaa cataaagccg ttttagatac aatgcgcgaa ctcgaacgcc aaggttttga
     7621 agtaacctac ctcggcgttc aagaaaacgg tttgattgat ttggaagaac tcaaagccgc
     7681 catccgcgac gataccatcc tgatttccgt aatgtgggta aacaacgaaa tcggcgtggt
     7741 gcaagacatt cccgccatcg gcgaaatctg ccgcgaacgt aaaatcgtct tccacgtcga
     7801 tgccgcccaa gcctgcggca aagtgcctgt cgatgtcgaa gccgccaaaa tcgacctgct
     7861 ctcgatgtcc gcgcacaaag tgtacggccc caaaggcatc ggcgcgctgt acgtccgccg
     7921 caaaccccgc gtccgcctcg aagcccaaat gcacggcggc ggccacgaac gcggcttccg
     7981 cagcggcaca ttaccgaccc accaaatcgt cggtatgggc gaagccttcc gcatcgccaa
     8041 agaagaactc gaacaagata tggcgcacta ccgcaaactg cgcgatatct tcctcaaagg
     8101 catcgaaggc atcgaagaag tctacatcaa cggcgacctc ggacaccgcg ccccgaacaa
     8161 cctgaacgtc agcttcaact tcgtcgaagg cgaaagcctg attatggcgg tgaaagaact
     8221 cgccgtatcc agcggctccg cctgcacctc cgcctcgctc gaacccagct acgtcctgcg
     8281 cgcgctcggc cgcaacgacg aactggcgca ctcatccctg cgtatcacct tcggtcgcat
     8341 gaccaccgaa gaagaagtgc aattcgccgc cgaactgatt aaatccaaaa tcggcaaact
     8401 gcgcgaactg tcgccgctgt gggaaatgtt caaagacggg attgatttga actcgattga
     8461 gtgggcggcg cattaattta tgaattagta cgatgatatg agtatagtag ataagatttt
     8521 caaatattct cgccacctcg attattgaga tatagtgaac ttcttatcta cggataaaat
     8581 ttttatagat agtttaatat ttaaatagaa cttaaaaaat tatgaaaata gccaatgcat
     8641 taattcacaa tacagtccgt attgaatgcc tttcagcaga cggccaatct atatcaacgg
     8701 gaacggcatt cttattcctt ttcgatttcg acaaaacagg ggtaattcca gttcttgtaa
     8761 caaataaaca tgttgttttt gtagagtccg caaaaaaaat agctattact ttgacaaaag
     8821 atgaaaatgg ctctccaaat cataaagaaa atataacttt tacaattgaa gattttatac
     8881 aaaattgttt acctcatcct gatgaaaata ttgatctatg cattgctcct gttggacatt
     8941 tttttaatca gctgattaat cataatttct cgccttttat aaaaggaata agagaatctg
     9001 atattatgca aactgaagaa atggatcaat tatcagcttt cgaagaagtt tttatgatag
     9061 gctatcctaa tggcttatgg gactcaaaaa acaacttacc aatatttaga acaggaatta
     9121 ctgctaccca tccagctatt gattttgaag ggaaaaaaga atttatgatt gatgttgcct
     9181 gtttcactgg ttcaagcggt tctccggtgt tcaaatatat acctgctggg ttaagaaaaa
     9241 tagaaaacga tgggtttgca attacagcag atgaaacatt taaattaatg ggaatcctat
     9301 atgctggacc tcagcaaaca atatcagggg aaatttatat aacaactgtt aataaaccaa
     9361 tttctgtatc ttctattcct attaatttag gttttgtcat aaaggcagaa aaacttttag
     9421 actttaaacc aattttaaag aattttttgg aaaaaaatag agaatgaaat tatattaaaa
     9481 gattaataaa tattagagac tggcaatcca ccttacctaa tctacattaa aaataaaaaa
     9541 gaccgtttga aacatcaaaa agattttttc aaacgaccta aaagccacca catgaaatca
     9601 aggatacaaa accatgaacg aacaagaact tgacctcgac aatctcgaca acctgcttga
     9661 cgattttgac ggcgttaccg tcgaaggcgg cgtcgattcg gaaaacgacg aaggctgcga
     9721 aggcggcgcg tgcaaaatct gactcaggcc gtctgaaaac cggcaaacaa accactttaa
     9781 actatcaaaa acattaagga aaccacatca tggcatacag cgataaagta atcgaccact
     9841 acgaaaatcc ccgcaacgtc ggtactttcg acaaaaacga cgaatctgtc ggcaccggca
     9901 tggtcggcgc gcccgcctgc ggcgacgtca tgcgcctgca aatcaaagtg aacgacgagg
     9961 gcatcatcga agatgcgaaa tttaaaactt acggctgcgg ctcggccatc gcttcgtcca
    10021 gcctgattac cgagtgggtt aaaggcaaaa gcctggatga cgcgctggca atcaaaaaca
    10081 gcgaaatcgc cgaggagttg gaattgccgc cggtaaaaat ccactgctcc atcttggctg
    10141 aagatgcggt aaaagcggcc gttgccgact accgcaaacg tcaggaaaac agataaagcc
    10201 cttcagacgg catcatcccg caatgccgtc cgaaccaccc gccgcttcag gtccgtccgg
    10261 ggcggtacaa caaggaagaa atatgattac ccttaccgag aatgccgcaa aacacatcaa
    10321 tgactatctc gccaaacgcg gcaaaggctt gggcgtacgc ttgggtgtga aaaccagcgg
    10381 ctgctcgggg atggcgtaca accttgaatt tgtcgacgaa gccgatggcg acgacctgat
    10441 tttcgaagga cacggcgcgc gcatttatat cgatccgaaa agcctggttt atctggatgg
    10501 cacgcaagtc gattacacca aagaaggttt gcaggaaggt ttcaaatttg aaaaccccaa
    10561 tgtcaaagac tcctgcggct gcggcgaaag cttccacgtt taaggcataa aaacggcggg
    10621 accgtatcaa aaccgtcccg ccattttttc gcttcctgcc tgttgtagct gcctttgcct
    10681 ttccttttcc gttccacctt gtgccggaac aaatcggatt tcactaaggc ttttaaagca
    10741 ttgtcgcgta ttttgccttt attgtgctgc actttgccgc ccatattcag tcctttcgtt
    10801 taagaagcgg cagattataa ggcaaaaaca gttttctgcc aaaatcttac atttatcatc
    10861 ctactatgtc ccaatatttc accctcttcc ggattgaacc cgctttcgat atcgacaccg
    10921 aaaacttgga acaaacctac cgcgccttgg ccgcccgttt ccatcccgat aaattcgctt
    10981 cagcttccgc ctttgagcaa aagcaggcag tgatgatgtc ttccaccatc aacgatgcct
    11041 accgcacctt gaaaaacccc atcgaccgcg ccgcctacct gctgaaaaca tcgggcatcg
    11101 atgccgacgc gccggagcat accgctttcg cccccgaatt ccttatgcag caaatggaat
    11161 ggcgcgaaac gctgatggag gcacgggcag gcaacgacct tgaatccttg aaaaatctcg
    11221 acaacgaaat ccgcgacgaa caagaaaaac tgttctgcgg tctgaaacag tcgtttgccc
    11281 gacaagatta cgacacagcc gcacaacaag tccgccaagg caggtttctc gacaaactcc
    11341 gcaacgaaat ttcctcggca ttataatccg caccgtgttt cagacggcgt aaccgccgca
    11401 ccgttccgcg tcaaaatatg ctaaaataag caacaatttt ttgccatacg aaacattgaa
    11461 accatgaccg acgcaaccat ccgccacgac cacaaattcg ccctcgaaac cctgccggta
    11521 agccttgaag acgaaatgcg caaaagctat ctcgactacg ccatgagcgt cattgtcggg
    11581 cgcgcgctgc cggacgttcg cgacggtctc aagccggtac accgccgcgt actgtacgcg
    11641 atgcacgagc tgaaaaacaa ctggaatgcc gcctacaaaa aatcggcgcg cattgtcggc
    11701 gacgtcatcg gtaaatacca cccccacggc gataccgccg tatacgacac catcgtccgt
    11761 atggcgcaaa atttcgctat gcgttatgtg ctgatagacg gacagggcaa cttcggatcg
    11821 gtggacgggc ttgccgccgc agccatgcgc tacaccgaaa tccgcatggc gaaaatttcc
    11881 cacgaaatgc tggcagacat tgaggaagaa accgtcaatt tcggcccgaa ctacgacggt
    11941 agcgaacacg agccgcttgt actgccgacc cgtttcccca cactgctcgt caacggctcg
    12001 tccggcatcg ccgtcggcat ggcgaccaat atcccgccgc acaacctttc tgataccgtc
    12061 aatgcctgcc tgcgcctgct cgatgcaccc gacaccgaaa tcgacgaact gatcgacatt
    12121 atccaagccc ccgacttccc gaccggggca accatctacg gcttgagcgg cgtgcgcgaa
    12181 ggctataaaa caggccgcgg ccgcgtcgtt atgcgcggta agacccatat cgaacccata
    12241 ggcagaaacg gcgaacgcga agccatcgtt atcgacgaaa tcccctatca ggtcaacaaa
    12301 gccaagctgg tcgagaaaat cggcgatttg gttcgggaaa aaacactgga aggcatttcc
    12361 gagctccgcg acgaatccga caaatccggt atgcgcgtcg ttatcgagct gaaacgcaac
    12421 gaaaatgccg aagtcgtctt aaaccaactc tacaaactga ctccgctgca agacagtttc
    12481 ggcatcaata tggtggtttt ggtcgacgga caaccgcgcc tgttgaacct gaaacagatt
    12541 ctctccgaat tcctgcgcca ccgccgcgaa gtcgttaccc gacgtacgct tttccggctg
    12601 aagaaggcac gccatgaagg gcatatcgcc gaaggcaaag cggtcgcact gtccaatatc
    12661 gatgaaatca tcaagctcat caaagaatcg cccaacgcag ccgaggccaa agacaaactg
    12721 cttgcgcgtc cttggcgcag cagcctcgtt gaagaaatgc tgacgcgttc cggtctggat
    12781 ttggaaatga tgcgtccgga aggattggct gcaaacatcg gcttgaaaga gcaaggttat
    12841 tacctgagcg agattcaggc agatgctatt ttacgcatga gcctgcgaaa cctgaccggc
    12901 ctcgatcaag aagaaattgt cgaaagctac aaaaacctga tgggtaaaat catcgacttt
    12961 gtggatatcc tctccaaacc cgaacgcatt acccaaatca tccgcgacga actggaagaa
    13021 atcaaaacca actatggcga cgaacgccgc agcgaaatca acccgttcgg cggcgacatt
    13081 gccgatgaag acctgattcc gcaacgcgaa atggtcgtta ccctgacaca tggcggctat
    13141 atcaaaaccc agccgaccac cgactatcag gcgcagcgtc gcggcgggcg cggcaaacag
    13201 gcggctgcca ccaaagacga agactttatc gaaaccctgt ttgttgccaa cacgcatgat
    13261 tatttgatgt gctttaccaa tttgggcaag tgtcattgga ttaaggttta caaactgccc
    13321 gaaggcggac gcaacagccg cggccgtccg attaacaacg tcatccagtt ggaagaaggc
    13381 gaaaaagtca gcgcgattct ggcagtacgc gagttccccg aagaccaata cgtcttcttc
    13441 gccaccgcgc agggaatggt gaaaaaagtc caactttccg cctttaaaaa cgtccgcgcc
    13501 caaggcatta aagccatcgc gctcaaagaa ggcgactacc tcgtcggcgc tgcgcaaaca
    13561 ggcggtgcgg acgacatcat gctgttctcc aacttaggta aagccatccg cttcaacgaa
    13621 tactgggaaa aatccggcaa cgacgaagcg gaagatgccg acatcgaaac cgaaatttca
    13681 gacggcatcg aagatgaaac cgccgacagc gaaaacgcac tgccgagcgg caaacacggt
    13741 gttcgcccgt ccggtcgcgg cagcggcggt ttgcgcggta tgcgcctgcc tgccgacggc
    13801 aaaatcgtca gcctgattac cttcgcccct gaaaccgaag aaagcggttt gcaagtttta
    13861 accgccaccg ccaacggata cggaaaacgc accccgattg ccgattacag ccgcaaaaac
    13921 aaaggcgggc aaggcaatat tgccattaac accggcgagc gaaacggcga tttggtcgcc
    13981 gcaaccttgg tcggcgaaac cgacgatttg atgctgatta ccagcggcgg cgtacttatc
    14041 cgcaccaaag tcgaacaaat ccgcgaaacc ggccgcgccg cagcaggcgt gaaactgatt
    14101 aacttggacg aaggcgaaac cttggtatcg ctggaacgtg ttgccgaaga cgaatccgaa
    14161 ctctccgacg cttctgtaat ttccaatgta accgaaccgg aagtcgagaa ctgaaaatca
    14221 tctcccgatg ccgtctgaag attcagacgg catttatttt atccctcatc cgtcatccag
    14281 cttctcacaa tatagcggat tatagtcaat taaaaacaag gggctgtcct agataactag
    14341 ggaaattcaa attaagttag agttgcccct atgagaaaaa gtcgtctaag ccggtataaa
    14401 caaataaact cattgaactg tttgtcgcag gtgtaactgc aagaacgaca gcagagttag
    14461 taggcgttaa taaaa
//
LOCUS       DAWGJV010000040        14252 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWGJV010000040 DAWGJV010000000
VERSION     DAWGJV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.040"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(199..1242)
                     /locus_tag="WHQ94_000868"
     CDS             complement(199..1242)
                     /locus_tag="WHQ94_000868"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ2128179.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSNGICPETVLRDYYRDSLFVDI
                     LPTGSAPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLDAIPLLP"
     gene            complement(1239..1520)
                     /locus_tag="WHQ94_000869"
     CDS             complement(1239..1520)
                     /locus_tag="WHQ94_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128180.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1772..2092
                     /locus_tag="WHQ94_000870"
     CDS             1772..2092
                     /locus_tag="WHQ94_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ2128181.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLLYNH
                     IIVD"
     gene            2111..2920
                     /locus_tag="WHQ94_000871"
     CDS             2111..2920
                     /locus_tag="WHQ94_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ2128182.1"
                     /translation="MPRLAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQ
                     LTAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHL
                     AVGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYE
                     AFSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKIS
                     VDELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            3339..3698
                     /locus_tag="WHQ94_000872"
     CDS             3339..3698
                     /locus_tag="WHQ94_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ2128183.1"
                     /translation="MERKNNIIELSIEDLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAATGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDSTNYC"
     gene            3759..4385
                     /locus_tag="WHQ94_000873"
                     /pseudo
     CDS             3759..4385
                     /locus_tag="WHQ94_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
     gene            4331..4486
                     /locus_tag="WHQ94_000874"
     CDS             4331..4486
                     /locus_tag="WHQ94_000874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128184.1"
                     /translation="MGMVSSDLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEA
                     DILHEKR"
     gene            complement(4735..4842)
                     /locus_tag="WHQ94_000875"
                     /pseudo
     CDS             complement(4735..4842)
                     /locus_tag="WHQ94_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4852..5154
                     /locus_tag="WHQ94_000876"
     CDS             4852..5154
                     /locus_tag="WHQ94_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128185.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5438..5677
                     /locus_tag="WHQ94_000877"
     CDS             5438..5677
                     /locus_tag="WHQ94_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ2128186.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            5738..7480
                     /gene="hrpB"
                     /locus_tag="WHQ94_000878"
     CDS             5738..7480
                     /gene="hrpB"
                     /locus_tag="WHQ94_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ2128187.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7590..13568
                     /gene="hrpA"
                     /locus_tag="WHQ94_000879"
     CDS             7590..13568
                     /gene="hrpA"
                     /locus_tag="WHQ94_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ2128188.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLA
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTGAGAAIVVIIVTALTYGYG
                     AAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVG
                     KALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGR
                     LATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAK
                     QFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKI
                     IAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQSI
                     MPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAIS
                     TCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINN
                     LANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQ
                     AISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALR
                     NFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            13587..13961
                     /locus_tag="WHQ94_000880"
     CDS             13587..13961
                     /locus_tag="WHQ94_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ2128189.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT         4101 a         3250 c         3434 g         3467 t
ORIGIN      
        1 aatcgaagcg tgtggaaatg tgtcggaaat tgctgaagct ggataaacta gattcccgct
       61 tttgcgggaa tgacggtatt ttagattttt gttttcgtgt gaatggatgg gtcaaaagcc
      121 gtccgaaccc aaaaaaacgt cgctttcaga cggcatcaaa aaccgcaggc acgatgcctg
      181 cggtttgctg tttgcgcttc aggggagcag ggggattgcg tccaagccgt gtgtttcctc
      241 cagtccgaac ataatgttca tattttggac tgcctgaccc gccgcgcctt tgacgaggtt
      301 gtcgatgacg gaaaggacga cccacacatc ggattgcgcc gcctgttgga tgctgatgcg
      361 gcagaggttt gcgccgcgca cgctgcgggt ttcgggggcg gaaccggtcg gcaggatgtc
      421 cacgaacagg ctgtcgcggt aatagtcgcg caggacggtt tcggggcaga tgccgtttga
      481 aaggtggagg taaacggtgg cgtgcatacc gcgtatcatc ggcgcgaggt gcggcgtgaa
      541 cacgaatcct tcggcgatgc cgtcctgaag cccggcgatg gtctgcctga tttcgggcag
      601 gtggcggtgt ccggctatgc cgtaggcttt gaagttgtcg ccggcttcgc acaacagcga
      661 accgacattg cctttcctgc ccgcgccgga cacgccggat ttgcagtcgg caatcagcgg
      721 cataccgggc ttcagacggc attgccgcaa cagcggcacg agcggtaggg atacgcaggt
      781 cgggtagcag ccggggttgg cgacgaggcg cgcctgtgcg acggcttcgc ggttgagttc
      841 gctcaatccg tacacggctt gggaaacgag gccgggggcg gcgtgggtca tgccgtacca
      901 gtgttcccag gtcggaatgt cccgtatgcg gaagtcggcg gaaaggtcga tgacgcgcac
      961 gccctgttcc aaaaggcgca gcgcgtcttt catagcgatg ccgttgggcg tggcgaagaa
     1021 gacgatgtcg cattgttcca aacctgcctc gtcgggcgtt tggaaggcga ggccgtacac
     1081 gccgcgcaga cttggaaagt aatcggcaac tgcagttccc gcttcgctgc ggctggttac
     1141 ggcggcgact tcgacatcgg gatgggcggc aagcaggcgc agcagttcca cgccggtgta
     1201 gcccgtcgcg ccgacaatgc cgactttgat ttttttactc atggtttttc ctttgtgtgg
     1261 ttggcgggta tgccgtccga acgctgtccg acggggtttt gattggcggc ggaaggacgg
     1321 tacacaaagc gtttgaaaag cgtgtctaat ttaatggcat ccgcctctgt ttttaaaacc
     1381 cagccctgcc gtccggaata aacataaccg taggcgcgaa agtcggcagg atatttcggt
     1441 gcctgcccca aacccaaacc gtatgcggcg aaagcggtgg tcgtggagaa ggaaagcagg
     1501 aggaggacga gggttttcat aaggttccaa accgaacggg gcgatgaagc ccgattatag
     1561 caaaaggtcg tctgaaaacc gttttcagac gaccttttat ttaattacaa taaaaatgtt
     1621 aacaacaaaa aacaaaccgc ttttttccgt ttggagacaa atttttaact aaaattgtca
     1681 acaattcctt gacgcacaca caaaaaactt taatatttgt aaatatatgt aacattataa
     1741 attttaaata ttttctgagt aaggggaagt aatgagcatc catctcgact tcggcattag
     1801 tcctaaaacg ttccgacaga cttatctgta tcaaaagccc aagctcttta aaggagcggt
     1861 tcggaatctc gaagccgcat cttggaaaga tatcaacgag atataccaac gagcagaccc
     1921 aaccgcaccg ctgtttcatc tgcgtaaaaa aggtgcaatt gttcctaaag aagaatacgt
     1981 cgaaagtttc gacgatttgg gcaaaactcg ctaccgtttt attaaatccg ttatctacga
     2041 acatatgaag aatggtgcgt cgttgctcta taaccatatt atagtggatt aaatttaaac
     2101 cagtacagcg ttgcctcgcc ttgccggcgc acatactatt gttagtggat atcttgcttt
     2161 tggcagcgac gaatcttata aaaaccattg ggacacccgc gatgtgtatg ccatccagct
     2221 tttcggcaag aaacgttggc aacttactgc ccctgatttc cctatgccat tgtatatgca
     2281 acagactaaa gatactgata tttccattcc tgaacatatc gatatggata ttatccttga
     2341 agcaggtgat gtcctctaca tcccgcgtgg ttggtggcac agacctatcc cgctcggctg
     2401 tgaaaccttc cacctcgctg tcggtacctt tccgcccaac ggctataatt acctcgagtg
     2461 gctaatgaag aaattcccca ccatagaaag tctgcgccac agtttctcag actgggagca
     2521 agataggacg cgtatcaacg atactgccgc acaaattgct gccatgattg ccgaccccgt
     2581 caattacgaa gccttcagtg aagacttcct cggcaaagaa cgcaccgata ccgcttttca
     2641 tctcgaacag ttcgcgaatc ccaacgctac tccgctttca gacgacgtca ggttgagact
     2701 aaatgccaat aatttggata cgttggaaaa gggatatttg attgggaatg ggatgaagat
     2761 aagcgtagat gaattgggga aaaaagtttt agaacacatc ggtaagaatg aaccgttatt
     2821 gttgaaaaat ctactggtta acttcaatca gggaaaacat gaagaagtta ggaagttgat
     2881 ttatcagttg atagagttag attttctgga acttttgtga gggattctat gaaaaactgg
     2941 aagcaattta catttttcgt aatattagta atagcttgtt atcaattgct atatttttta
     3001 tcagatatgt ttcttctcga ctacattaac aaatatagtt ggaatttgaa ttttattcag
     3061 ggtactttga atttttttca atatatctgc catatgtttt tgttagccga atttttagga
     3121 atgccaacca atgaaagact aggctatgaa atcaaaattt gtgtataagt tactggtgct
     3181 tgcaaagtac ctgtatattt taatttgtat atcaatacca atcgttttta tttatgaaag
     3241 ctaccaagga gatgatgttg attttatgat ttatattcgt gcttttatta ttttttccat
     3301 attgggtgta ttagctcaat attctatgtc taattttttt ggaaaggaaa aataacatca
     3361 ttgaactatc cattgaggat ctggagctaa tttatggcgg aggcttaggg gagagaatcc
     3421 ttgaaggagg agcaaatggt gcacaaatag gaggatcaat tggtgcaggt attggtctgg
     3481 gtgtcggtat agtttctggt ggtattcctg ccggaccggc tattattctc ggtggcacag
     3541 ttggtgctgc tgttggtgct gcaactggta tgggtgttgc agcatatgag tattaccaag
     3601 aaactcagcc caaagggaca gtaacggtcg gtccgatttc tgcttttgct gtggacaaat
     3661 caggaaacgg ctacactgac agcacaaatt actgctgata tttctcatcg tccagacaat
     3721 ctctaggggt cgtctgaaac tttcttaact tcaattttat gaatagaccc aagcaaccct
     3781 tcttccgtcc cgaagtcgcc gttgcccgcc aaaccagcct gacgggtaaa gtgattctga
     3841 cacgaccgtt gtcattttcc ctatggacga catttgcatc gatatctgcg ttactgatta
     3901 tcctgttttt gatatttggt aactatacgc gaaagacaac agcggaggga caaattttac
     3961 ctgcatcagg cgtaatcagg gtgtatgcac cggatacggg gacaattaca gcgaaattcg
     4021 tggaagatgg agaaaaggtt aaggctggcg acaagctatt tgcgctttcg accttacgtt
     4081 tcggcgcagg aggtagcgtg cagcagcagt tgaaaacgga ggcagttttg aagaaaacgt
     4141 tggcagaaca ggaactgggt cgtctgaagc tgatacacgg gaatgaaacg cgcagcctta
     4201 aagcaactgt cgaacgtttg gaaaaccaga aactccatat ttcgcaacag atagacggtc
     4261 agaaaaggcg cattagactt gcggaagatc agtagcaaaa acggcactgg gcagacagga
     4321 attgtcggga ttgggcatgg tatcctccga tttggcgaag agcaacgaac ctgtttatct
     4381 tgtgaaaata aaacccgaca aatcaaccat cactgcatac ggtgaggaaa aaccgctgca
     4441 aatcggcatg acgttggaag cagacatcct acacgagaaa cggtgattgt acgaatgggt
     4501 attggagctg atttatagta tgtcgggcaa actgtaagac ttgaggctgt aaagataaag
     4561 aagttgtctg aaaccttttc agacgaccag aagacaatat ataaaaacac aaacttcaaa
     4621 actgcctgaa accatcccct tttacaggca ctccgtttgt aaaagcaagg atagggcatc
     4681 tgcccttttg attgtttgat taacgataca aggagtttca aaatgagagt tttatagtgg
     4741 attaaattta aaccagtaca gcgttgcctc gccttgccgt accatttgta ctgtctgcgg
     4801 cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat gtgttcatga aatgacttgg
     4861 gtcggaggct caggtaatgc gcaacaaagc tcatattatt gcgaaatttg cgaatctgca
     4921 gggcttaacg atacgggaaa tcctgataaa tctttaggat tgccaaacaa tacgttcagt
     4981 aatccgcctg gttggggagc tacaatcgga gctttagcag gtagccgcat agggatgcct
     5041 gaatttggta cgtttgcgag ccatgccatt gaaaatttcg actggtcatg gtatcgacgt
     5101 tatagggaaa ttgccgaaac gattgaacga gaatattcag gcggtttgcc ttaatagttg
     5161 aggaggtcat gatgtttgcc aaacattatc aattcatcgc actcggcatc atgctgcttc
     5221 tttatatgtt gattctctat acgaccgatt tttccaatct gacgtattgg atgctgtttt
     5281 ttatctgttt tattacagga aaaatattag ctcgtttgtt agagaaaagc tttaaataaa
     5341 atagcagcta gtcgcaaaag gtcgtctgaa accttttcag gcggcctttc taaaatacat
     5401 ccaacttcct aatccctatt tttcaaaaag gaaatctatg ccccatctgc aaaacctgtc
     5461 tttgggctta aagaaaaagc tgcctgttat cctgcaaaca gaaatatcag aatgcggctt
     5521 ggcatgtctg gcggctgtgg cgggatttca tggtttccat acgaatttac gcgcactgcg
     5581 ttcaaaatac tgtccgagac ctttgcaaaa ttccccaaaa tcccctaaat gtcttggtgg
     5641 gaattttggg gaattttgca aaggtctcat tctataactg taaatacttt taaatttatg
     5701 acaaaatagt aaatattgct aaaataatat tgatgtcatg aaattttttc ctgctccatg
     5761 tctgttggtt atcctggctg tcatacccct taaaacctta gctgccgatg aaaacgatgc
     5821 agaacttatc cgttccatgc agcgtcagca gcacatagat gctgaattgt taactgatgc
     5881 aaatgtccgt ttcgagcaac cattggagaa gaacaattat gtcctgagtg aagatgaaac
     5941 accgtgtact cgggtaaatt acattagttt agatgataag acggcgcgca aattttcttt
     6001 tcttccttct gtgctcatga aagaaacagc ttttaaaact gggatgtgtt taggttccaa
     6061 taatttgagc aggctacaaa aagccgcgca acagatactg atcgtgcgtg gctacctcac
     6121 ttcccaagct attatccaac cacagaatat ggattcggga attctgaaat tacgggtatc
     6181 agcaggcgaa ataggggata tccgctatga agaaaaacgg gatgggaagt ctgccgaagg
     6241 cagtattagt acattcaata acaaatttcc cttatatagg aacaaaattc tcaatcttcg
     6301 cgatgtagag cagggcttgg aaaacctgcg tcgtttgtcg agtgttaaaa cagatattca
     6361 gattataccg tccgaagaag aaggcaaaag cgatttacag atcaaatggc agcagaataa
     6421 acccatacgg ttcagtatcg gtatagatga tgcgggcggc aaaacgaccg gcaaatatca
     6481 aggaaatgtc gctttatcgt tcgataaccc tttgggctta agcgatttgt tttatgtttc
     6541 atatggacgc ggtttggtgc acaaaacgga cttgactgat gccaccggta cggaaactga
     6601 aagcggatcc agaagttaca gcgtgcatta ttcggtgccc gtaaaaaaat ggctgttttc
     6661 ttttaatcac aatggacatc gttaccacga agcaaccgaa ggctattccg tcaattacga
     6721 ttacaacggc aaacaatatc agagcagcct ggccgccgag cgcatgcttt ggcgtaacag
     6781 acttcataaa acttcagtcg gaatgaaatt atggacacgc caaacctata aatacatcga
     6841 cgatgccgaa atcgaagtgc aacgccgccg ctctgcaggc tgggaagccg aattgcgcca
     6901 ccgtgcttac ctcaaccgtt ggcagcttga cggcaagttg tcttacaaac gcgggaccgg
     6961 catgcgccaa agtatgcctg caccggaaga aaacggcggc gatattcttc caggtacatc
     7021 tcgtatgaaa atcattactg ccggtttgga cgcagccgcc ccatttattt taggcaaaca
     7081 gcagtttttc tacgcaaccg ccattcaagc tcaatggaac aaaacgccgt tggttgccca
     7141 agataaattg tcaatcggca gccgctacac cgttcgcgga tttgatgggg agcagagtct
     7201 tttcggagag cgaggtttct actggcagaa tactttaact tggtattttc atccgaacca
     7261 tcagttctat ctcggtgcgg actatggccg cgtatctggc gaaagtgcac aatatgtatc
     7321 gggcaagcag ctgatgggtg cagtggtcgg cttcagagga gggcataaag taggcggtat
     7381 gtttgcttat gatctgtttg ccggcaagcc gcttcataaa cccaaaggct ttcagacgac
     7441 caacaccgtt tacggcttca acttgaatta cagtttctaa cctctgaatt tttttactga
     7501 tatttagacg gtctttcctt atcctcagac cgtcaaactt tacctacgta cttggcgcgc
     7561 agtacgttca tcttcaaaat ggaatagaca tgaataaagg tttacatcgc attatcttta
     7621 gtaaaaagca cagcaccatg gttgcagtag ccgaaactgc caacagccag ggcaaaggta
     7681 aacaggcagg cagttcggtt tctgtttcac tgaaaacttc aggcgacctt tgcggcaaac
     7741 tcaaaaccac ccttaaaacc ttggtctgct ctttggtttc cctgagtatg gtattgcctg
     7801 cccatgccca aattaccacc gacaaatcag cacctaaaaa ccagcaggtc gttatcctta
     7861 aaaccaacac tggtgccccc ttggtgaata tccaaactcc gaatggacgc ggattgagcc
     7921 acaaccgcta tacgcagttt gatgttgaca acaaaggggc agtgttaaac aacgaccgta
     7981 acaataatcc gtttctggtc aaaggcagtg cgcaattgat tttgaacgag gtacgcggta
     8041 cggctagcaa actcaacggc atcgttaccg taggcggtca aaaggccgac gtgattattg
     8101 ccaaccccaa cggcattacc gttaatggcg gcggctttaa aaacgtcggc cgcggtatct
     8161 taaccaccgg tacgcctcaa atcggcaaag acggtgcctt gacgggattt gacgtgcgcc
     8221 aaggcacatt gaccgtcggt acgtcaggtt ggaacgacaa aggcggagcc gattacaccg
     8281 aagtgcttgc ccgtgcggtt gctttgcagg ggaaattgca gggtaaaaac ctggcggttt
     8341 cgaccggcgc acagaaagta gattacgcca gcggtgaaat cagcgcagga acggcagcag
     8401 gtacgaaacc gaccgttgcc ctcgatactg ccgcattggg tggtatgtac gcagacagca
     8461 ttaccctgat tgccaatgaa aaaggcgtag gcgtcaaaaa tgccggcaca ctcgaagcgg
     8521 ccaagcaatt gattgtgact tcgtcaggcc gcattgaaaa cagcggccgc atcgccacca
     8581 ctgccgacgg caccgaagct tcaccgactt atctctccat cgaaaccacc gaaaaaggag
     8641 cggcaggcac atttatctcc aatggtggtc ggatcgagag caaaggctta ttggttattg
     8701 agacgggaga agatatcagc ttgcgtaacg gagccgtggt gcagaataac ggcagtcgcc
     8761 cagctaccac ggtattaaat gctggtcata atttggtgat tgagagtaaa actaatgtga
     8821 acaatgccaa aggctcggct aatctgtcgg ccggcggtcg tactacgatc aatgatgcta
     8881 ctattcaagc gggcagttcc gtgtacagct ccaccaaagg cgatactgaa ttgggtgaaa
     8941 atacccgtat tattgctgaa aacgtaaccg tattgtccaa cggcagtatt ggcagtgctg
     9001 ctgtaattga ggctaaagac actgcacaca ttgaagcagg caaaccgctt tctttggaag
     9061 cttcaacagt tacctccgat atccgcttaa acggaggcag tatcaagggc ggcaagcagc
     9121 ttgctttact ggcagacgat aacattactg ccaaaactac caatctgaat actcccggca
     9181 atctgtatgt tcatacaggt aaagatctga atttgaatgt tgataaagat ttgtctgccg
     9241 ccagcatcca tttgaaatcg gataacgctg cccatattac cggcaccagt aaaaccctca
     9301 ctgcctcaaa agacatgggt gtggaggcag gctcgctgaa tgttaccaat accaatctgc
     9361 gtaccaactc gggtaatctg cacattcagg cagccaaagg caatattcag cttcgcaata
     9421 ccaagctgaa cgcagccaag gctctcgaaa ccaccgcatt gcagggcaat atcgtttcag
     9481 acggccttca tgctgtttct gcagacggtc atgtatcctt attggccaac ggtaatgccg
     9541 actttaccgg tcacaatacc ctgacagcca aggccgatgt caatgcagga tcggttggta
     9601 aaggccgtct gaaagcagac aataccaata tcacttcatc ttcaggagat attacgttgg
     9661 ttgccggcaa cggtattcag cttggtgacg gaaaacaacg caattcaatc aacggaaaac
     9721 acatcagcat caaaaacaac ggtggtaatg ccgacttaaa aaaccttaac gtccatgcca
     9781 aaagcggggc attgaacatt cattccgacc gggcattgag catagaaaat accaagctgg
     9841 agtctaccca taatacgcat cttaatgcac aacacgagcg ggtaacgctc aaccaagtag
     9901 atgcctacgc acaccgtcat ctaagcatta ccggcagcca gatttggcaa aacgacaaac
     9961 tgccttctgc caacaagctg gcggctaacg gtgtattggc actcaatgcg cgctattccc
    10021 aaattgccga caacaccacg ctgagagcgg gtgcaatcaa ccttactgcc ggtaccgccc
    10081 tagtcaagcg cggcaacatc aattggagta ccgtttcgac caaaactttg gaagataatg
    10141 ccgaattaaa accattggcc ggacggctga atattgaagc aggtagcggc acattaacca
    10201 tcgaacctgc caaccgcatc agtgcgcata ctgacctgag catcaaaaca ggcggaaaat
    10261 tgctgttgtc tgcaaaagga ggaaatgcag gtgcgcctag tgctcaagtt tcctcattgg
    10321 aagcaaaagg caatatccgt ctggttacag gagaaacaga tttaagaggt tctaaaatta
    10381 cagccggtaa aaacttggtt gtcgccacca ccaaaggcaa gttgaatatc gaagccgtaa
    10441 acaactcatt cagcaattat tttcctacac aaaaagcggc tgaactcaac caaaaatcca
    10501 aagaattgga acagcagatt gcgcagttga aaaaaagctc gcctaaaagc aagctgattc
    10561 caaccctgca agaagaacgc gaccgtctcg ctttctatat tcaagccatc aacaaggaag
    10621 ttaaaggtaa aaaacccaaa ggcaaagaat acctgcaagc caagctttct gcacaaaata
    10681 ttgacttgat ttccgcacaa ggcatcgaaa tcagcggttc cgatattacc gcttccaaaa
    10741 aactgaacct tcacgccgca ggcgtattgc caaaggcagc agattcagag gcggctgcta
    10801 ttctgattga cggcataacc gaccaatatg aaattggcaa gcccacctac aagagtcact
    10861 acgacaaagc tgctctgaac aagccttcac gtttgaccgg acgtacgggg gtaagtattc
    10921 atgcagctgc ggcactcgat gatgcacgta ttattatcgg tgcatccgaa atcaaagctc
    10981 cctcaggcag catagacatc aaagcccata gtgatattgt actggaggct ggacaaaacg
    11041 atgcctatac cttcttaaaa accaaaggta aaagcggcaa aatcatcaga aaaaccaagt
    11101 ttaccagcac ccgcgaccac ctgattatgc cagcccccgt cgagctgacc gccaacggta
    11161 tcacgcttca ggcaggcggc aacatcgaag ctaataccac ccgcttcaat gcccctgcag
    11221 gtaaagttac cctggttgcg ggtgaagagc tgcaactgct ggcagaagaa ggcatccaca
    11281 agcacgagtt ggatgtccaa aaaagccgcc gctttatcgg catcaaggta ggtaagagca
    11341 attacagtaa aaacgaactg aacgaaacca aattgcctgt ccgcgtcgtc gcccaaactg
    11401 cagccacccg ttcaggctgg gataccgtgc tcgaaggtac cgaattcaaa accacgctgg
    11461 ccggtgccga cattcaggca ggtgtaggcg aaaaagcccg tgtcgatgcg aaaattatcc
    11521 tcaaaggcat tgtgaaccgt atccagtcgg aagaaaaatt agaaaccaac tcaaccgtat
    11581 ggcagaaaca ggccggacgc ggcagcacta tcgaaacgct aaaactgccc agcttcgaaa
    11641 gccctactcc gcccaaattg tccgcacccg gcggctatat cgtcgacatt ccgaaaggca
    11701 atctgaaaac cgaaatcgaa aagctgtcca aacagcccga gtatgcctat ctgaaacagc
    11761 tccaagtagc gaaaaacatc aactggaatc aggtgcagct tgcttacgac agatgggact
    11821 ataaacagga aggcttgacc ggagccggtg cagcgattgt ggtgattatt gtaaccgctc
    11881 taacttatgg atacggagcg gctgcagcgg gtagcgtaac tgccgcagga agtagtacag
    11941 ccgcagctgc aacaacgaca gcggcagcaa ctaccactgt ttctactgca gctgccatgc
    12001 aaaccgcagc tttagcctcc ttgtatagcc aagcagctgt agccatcatc aataataaag
    12061 gcgatgtcgg caaagcgttg aaagatctcg gcaccagtga tacggtcaag cagattgtta
    12121 cttccgcact gacggcgggt gcattaaacc agatgggagc agatattgcc caattgaaca
    12181 gcaaggtaag aaccgaactg ttcagcagta cgggcaatca aaccattgcc aaccttggag
    12241 gcagattggc caccaacctc agcaatgcag gtatctcagc tggtatcaat accgccgtta
    12301 acggcggcag cttgaaagac aacttaggca atgccgcatt gggagcattg gttaatagct
    12361 tccaagggga agccgccagc aaaatcaaaa caaccttcag cgacgattat gttgccaaac
    12421 agttcgccca cgctttggcg ggttgtgtta gcggactggt acagggaaaa tgtaaagatg
    12481 gggcaattgg cgcagcagtt ggggaaatcg tagctgaatc catacttggc ggcagaaacc
    12541 ctgctacact cagcgatgca gaaaagcata aagttatcag ttactcgaag attattgcgg
    12601 gcagcgtggc ggcactcaac ggcggcgatg tgaatactgc ggcgaatgcg gctgaggtag
    12661 ctgtggtgaa taatgctttg aattttgaca gcacccctac caatgcgaaa aagcatcaac
    12721 cgcagaagcc cgacaaaacc gcactggaaa aaatcatcca aagtattatg cctgcacatg
    12781 cggcaggtgc gatgactaat ccgcaggata aggatgctgc catttggata agcaatatcc
    12841 gtaatggcat cacaggcccg attgtgatta ccagctatgg ggtttatgct gcaggttgga
    12901 cagctccgct gatcggtaca gcgggtaaat tagctatcag cacctgcatg gctaatcctt
    12961 ctggttgtac tgtcatggtc actcaggctg ccgaagcggg cgcgggaatc gccacgggtg
    13021 cggtaacggt aggcaacgct tgggaagcgc ctgtgggggc gttgtcgaaa gcgaaggcgg
    13081 ccaagcaagc tgctcctaaa gaaacaataa acaatttggc aaatttagcc aaagcagaac
    13141 agcagatttt attccgtatt gcccaacgcg atacgcaact ggatgcatgg aagacgggat
    13201 ttaacaatag agtaaggaaa ggagcaggct tgcttgatgc aagtaatatt ccgataacca
    13261 ttaacggaaa aaccatcaaa cctgtacaag ccataagctt aaagggagca cccgtttaca
    13321 gcggcgtaag cgaacaggag atttttgcgc tttatcggca gatgactggc cagaatccga
    13381 attttagagt tttgcctgac ggaagattag caaatggcat tatcagtact ggagaatggg
    13441 caggaacaaa aattgcatta agaaattttt caaaaacaga gaattcaact caagcacgat
    13501 ggacattaga tttgcagaat cctccatcat ttattaaagg tactaaattg gagcttaaat
    13561 tccaataatt tacaaaggat tttaccgtgg atgagaaaca aaaaattaag attcttgatt
    13621 ttcaaatcga tttatcctca atttttaact cttataaaaa tcaaatgggt attaatattc
    13681 aagataaaaa cttaaaaaac aatttctgtt cttttatgga agaactgtta aatgacggtt
    13741 caatccgttt acatgattat accgacggta tcggaattcc tctaactgga acttcaaaag
    13801 aacaagtgca gaaattgaaa gacatatggc ctactttgga agatgcccaa gcaatatggc
    13861 ctgaagaccc ttggtattac ttagaatggc tttggtggga tattgcgtgt ccaatagatt
    13921 tggccgattt gccgaatatt gatatttatg agcaagcgta ggtatggtta gccgccttta
    13981 gcggcgtaac cgtacgcata tcagcaaact ttataaaata acaaggccgt ctgaaatctg
    14041 tttttcaact ttttcagacg gccttgcaac ttggcatttc attcgtacgg ttacgccgct
    14101 aaaggcggct aaccgtacct acgagctctg ataaaaatga tttatggaag caagctgtag
    14161 cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctt cagcgcgcac gcgttccatg
    14221 atttacggct caatgccgtc tgaaaagctc aa
//
LOCUS       DAWGJV010000041        14079 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWGJV010000041 DAWGJV010000000
VERSION     DAWGJV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.041"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(53..361)
                     /locus_tag="WHQ94_000881"
     CDS             complement(53..361)
                     /locus_tag="WHQ94_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128190.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WHQ94_000882"
     CDS             complement(345..647)
                     /locus_tag="WHQ94_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ2128191.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WHQ94_000883"
     CDS             complement(721..3084)
                     /locus_tag="WHQ94_000883"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ2128192.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFTG
                     VVIAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3139..4164)
                     /locus_tag="WHQ94_000884"
     CDS             complement(3139..4164)
                     /locus_tag="WHQ94_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2128193.1"
                     /translation="MVSAIDSLKKNVNYNYVNSSNTHTIQIVHVEKPEGPIKIKRGDT
                     ETSISKQAIWRLADALSTGEPVNVDRVFGASYNFRSALEALLAHTPEIYWCKLQRIQP
                     GLNKSEVVEGHKHIIWLPDRPHEKGVMKEYETDVVISEIPSNNVVYDALSLPSTHSEI
                     DIDVKRRHVQIQIALSAIGFQLGFRTWIARNDQGISYGDKKIADLDGIVQKIESEKLI
                     SSFDGAVQAAMNIDCIWFRNGKLMPAVMEVEHSTGVRSGLARMKQLKDLLPPYANTRW
                     VIVAPDEDRDKVFKEANVPMFKNLDTQYFPYSAVEELYSLCIRRKLTNKAVNEEFLDC
                     FMEKCVM"
     gene            complement(4166..5260)
                     /locus_tag="WHQ94_000885"
     CDS             complement(4166..5260)
                     /locus_tag="WHQ94_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ2128194.1"
                     /translation="MIFSFFSGAGFLDLGFELSGFDIAFVNEVHPPFLEAYKYSRSRM
                     DIPKPKYGYFKGSIDECLYAEKAKDLAGWVKKEKQNGIIVGFIGGPPCPDFSIAGKNK
                     GKDGENGKLSQSYVDLICKNQPDFFVFENVKGLYRTAKHREFFNALKRQLSDFGYVCT
                     EKLINAIEYGVPQDRERIILVGFLSQHVDALQKFDWDAHISFPDALEKDWPTTEEVGR
                     VVSQPANIYPELTVQYWFNRNGVDTHPNASKHFQPRAGLEKFQTISEGDDKKKSYKRL
                     HRWRYSPTAAYGNNEVHIHPYLPRRISAAEALAIQSLPKEFELPDNMTLSNMFKTIGN
                     GVPFLAAKGIAMTLKSYLENHYERTKTDGC"
     gene            complement(5331..5777)
                     /locus_tag="WHQ94_000886"
     CDS             complement(5331..5777)
                     /locus_tag="WHQ94_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ2128195.1"
                     /translation="MDRLTPEQRKKCMQSNKSKGTKPELALAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQRKAEIKGNREFWIAKIERNIRRDMEVTG
                     RLKVEGWAVLRFWSNDVVKNTTCCAEKVRQAVRDKQGLSKFQTREK"
     gene            complement(6101..7093)
                     /gene="pheS"
                     /locus_tag="WHQ94_000887"
     CDS             complement(6101..7093)
                     /gene="pheS"
                     /locus_tag="WHQ94_000887"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ2128196.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSGEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
     gene            complement(7439..7798)
                     /gene="rplT"
                     /locus_tag="WHQ94_000888"
     CDS             complement(7439..7798)
                     /gene="rplT"
                     /locus_tag="WHQ94_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ2128197.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKSALAA"
     gene            complement(7811..8008)
                     /gene="rpmI"
                     /locus_tag="WHQ94_000889"
     CDS             complement(7811..8008)
                     /gene="rpmI"
                     /locus_tag="WHQ94_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ2128198.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(8155..8676)
                     /gene="infC"
                     /locus_tag="WHQ94_000890"
     CDS             complement(8155..8676)
                     /gene="infC"
                     /locus_tag="WHQ94_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ2128199.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(8694..10607)
                     /gene="thrS"
                     /locus_tag="WHQ94_000891"
     CDS             complement(8694..10607)
                     /gene="thrS"
                     /locus_tag="WHQ94_000891"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ2128200.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(10660..10736)
                     /locus_tag="WHQ94_000892"
     tRNA            complement(10660..10736)
                     /locus_tag="WHQ94_000892"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10700..10702),aa:Val,seq:gac)
     gene            10915..12030
                     /gene="tgt"
                     /locus_tag="WHQ94_000893"
     CDS             10915..12030
                     /gene="tgt"
                     /locus_tag="WHQ94_000893"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ2128201.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            12040..13122
                     /locus_tag="WHQ94_000894"
     CDS             12040..13122
                     /locus_tag="WHQ94_000894"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ2128202.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            13314..13715
                     /locus_tag="WHQ94_000895"
     CDS             13314..13715
                     /locus_tag="WHQ94_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ2128203.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(13778..14068)
                     /locus_tag="WHQ94_000896"
     CDS             complement(13778..14068)
                     /locus_tag="WHQ94_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128204.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3475 a         3804 c         3188 g         3612 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacagctctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgactccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta gggttacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccacccc attgttcggg cattgccgcg ccgtaccaca gaccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgtgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc ggcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcagtccg acctgcttgg cttcgggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgt tccatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacgatctga accaacccac ccgtacccgc
     2881 atcgacttgg gtaacgttca aacggtcggc atcaggatgt ttttcaacgg atttcacttc
     2941 ggcaattacc acacccgtaa acgcaggcgc ggcagtctcg gcttcttcca cttccaagcc
     3001 ggacatcgtt aacaggtgtt ccagcttatc ggcggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatattttt cctaaggatt aaatttgatt ggacaactta
     3121 agggtaaccg agtgtaaatt acattacaca tttttccata aagcagtcta aaaattcttc
     3181 attgacagct ttatttgtca atttccggcg aatgcacaag gaatacaact cttcaactgc
     3241 ggaataagga aaatattggg tatctaaatt tttaaacatc ggaacattcg cttctttaaa
     3301 aactttatcc ctatcttcat caggagcaac gataacccag cgcgtattag catatggtgg
     3361 caacaggtct ttcaactgct tcatccgtgc aaggccggaa cgtacacccg tactatgttc
     3421 cacttccatc accgccggca tcagttttcc attcctgaac cagatgcaat caatattcat
     3481 tgccgcctga accgcgccgt caaacgaact gatcagtttc tcgctttcta tcttttgtac
     3541 gataccatcc aaatcagcaa ttttcttatc cccataacta ataccttggt cgtttctcgc
     3601 aatccacgtc ctgaaaccaa gttgaaaacc tattgctgaa agggcaatct ggatttgcac
     3661 gtggcggcgt tttacatcta tatctatttc tgaatgggtt gaaggaaggg ataaagcatc
     3721 gtaaaccaca ttgttggagg ggatttccga aataacgaca tctgtctcat attccttcat
     3781 tacgcctttt tcatgtggcc tatcgggaag ccaaataatg tgcttgtgcc cttccaccac
     3841 ctcgctttta ttcagacccg gttgtatccg ctgcaatttg caccagtaaa tttcaggtgt
     3901 atgggcaagc aatgcctcca gtgcggaacg aaagttataa cttgcgccaa acaccctgtc
     3961 cacattgaca ggctcccccg tggacaacgc atctgccaac cgccaaattg cttgtttcga
     4021 tatcgaagtt tccgtatctc cccttttaat cttaatcgga ccttccggtt tttcaacatg
     4081 gacgatttgt atggtatgcg tatttgagct gttcacatag ttataattga catttttttt
     4141 caagctatca atcgcactga ccaaattagc agccgtcagt ttttgtccgc tcatagtgat
     4201 tctccaaata agattttaat gtcatcgcta tcccttttgc tgccaaaaac ggtacaccgt
     4261 tgcctatagt tttgaacata ttgctcaatg tcatgttatc aggcagctca aactccttcg
     4321 gcaaagactg tattgccaat gcctccgcag cggaaatcct tcttggcaga tagggatgaa
     4381 tgtggacttc gttgtttccg tatgcagcag tcggagaata acgccagcgg tgtaggcgtt
     4441 tataggactt ctttttatca tccccttcgc ttatggtttg aaatttttct aaacccgctc
     4501 ttggttggaa gtgtttgctc gcattcgggt gtgtatccac cccattgcga ttgaaccaat
     4561 actgaacggt caactcaggg tatatgtttg caggctgact gaccactctt cctacttctt
     4621 ctgtcgtagg ccaatctttt tccaaggcat cagggaatga aatatgagca tcccaatcaa
     4681 acttctgcaa agcatcaaca tgctgactca aaaaacccac taggataatc ctttccctgt
     4741 cttgcggtac gccgtattcg atggcattga tgagtttttc agtgcataca tatccaaaat
     4801 cagaaagttg ccgtttcaac gcattgaaaa actccctatg cttggcagtc ctatatagtc
     4861 ctttcacatt ctcaaataca aaaaaatcag gctggttttt acaaattaaa tccacataac
     4921 tttgtgataa cttaccattc tccccatcct tacctttatt tttccctgca atagaaaaat
     4981 ccggacatgg gggacctcca ataaatccga caataatgcc attttgtttt tcctttttaa
     5041 cccagccagc caaatctttt gctttctcag cataaagaca ttcgtcaata ctacccttaa
     5101 aatatccgta tttaggcttc gggatatcca ttctgcttcg agagtattta tatgcttcca
     5161 aaaatggggg atggacttca ttaacgaagg caatatcaaa tcctgacaac tcgaagccca
     5221 aatccaaaaa gcccgcacca gagaaaaaag aaaaaatcaa agtttttttt ctttcctgcc
     5281 gtatgcttat atccaaattg cagcttttat ccgtcaactg gaaggggaga ttatttttct
     5341 ctcgtttgaa attttgacaa tccctgtttg tcgcggacgg cttgtctgac tttttcggca
     5401 cagcaggttg tatttttaac gacatcatta ctccaaaaac gtaaaaccgc ccagccctcg
     5461 accttcagac ggcctgttac ttccatatcc cttcggatat tgcgttctat cttggcaatc
     5521 caaaattcgc ggttgccctt tatttccgcc tttctctgtt cccaatcctt gccgtgccaa
     5581 aactcgccat caacaaacac ggcaacttta tattttttaa atgaaaaatc aggcttccca
     5641 aaaatgcttc cgctattttt ccgatacctg agccccaaag cccacatcgc cttcgccaac
     5701 gcaagctcgg gcttcgtccc cttgcttttg ttggactgca tacatttttt gcgctgttcg
     5761 ggggttaatc tgtccatttc gtgaggcctg aaacgcgttt atacgaaagc gcattcattt
     5821 tacgcggcat cgttgcggat tccggcagtt ttgcacccct tgcaaggaaa caacaaaacc
     5881 caatcgcgca ggaagccacc tgtttcgtca ggttttccct gcaaagccgc aaggttcgca
     5941 gccggacgga ctcaaaatta ccaagcatcc catccgcaca aatcttcaga acgcatcaat
     6001 tcatctgccg aaatcccgcc gaaatcttgc cgcaggcttt ttcagcccgt ccggccggac
     6061 aatatgcggg ttgcactccg cttgcaggct gcccgcacca ttatttcgca aactgcttca
     6121 aaaaattcaa atcgttatca aaaaacaagc gcaaatcgtt cacgttgtaa cgcagcatcg
     6181 cgaaacggtc gagaccgata ccgaaagcga aaccggtgta tttttcggga tcgatattca
     6241 catttctcaa cacgttggga tgcaccatgc cgcagccgcc gacttccagc catttgccgt
     6301 tttcgcccat aatgtcgatt tcgccagacg gttcggtgaa cgggaagaaa gacgggcgga
     6361 aacgtacttg caaatcatcg cgttcaaaga agcgacggat aaaatccgtg aacactgctt
     6421 ttaagtcggc aaaagttaca ccctcttcta cccacaaacc ttccgcctga tggaacatag
     6481 gcgagtgcgt ggcatcgctg tccacacggt aaacgcggcc gggggcgata atgcggatgg
     6541 gcggctcttt tttatcgagc atatagcgga tttgaatcgg ggaagtgtgc gtacgcaaaa
     6601 catcaccgtt ttcaacgtaa aacgtatcct gcatcgcacg ggcaggatgg tttgcaggga
     6661 tgttcagggc ttggaaattg tgaaaatcgt cttcgatttc aggcccgtcc gccacttcga
     6721 aacccattcc gtgaaagagt tcgaccacac gttgcaaggt cagggttacg ggatgcaggc
     6781 tgccgccttc ctgagcgcgt ccgggcaggg taatgtcgag ggcttcggcg gcaagtcggg
     6841 cttgcagctt ggcttcgttg agggcatcgc gtttggcatt aaaagccgtc tgaaaccggt
     6901 ttttgcattc attgatatgc gcacctatgg ttttgcgctc ttcaggcgac atttgcccca
     6961 aagttttcag aagtccggtc aactcgccgg ttttgccaag ataacgggct ttgatttgtt
     7021 ctagagcgtt gaagtcttgc gcagcttcta ctgcggcaat gccttctgca acgatgcggt
     7081 ttacattttc cataatatca agtctgtcaa ttaatgatat gccacctgaa acaacgaggc
     7141 cgcctgtata gcctgacata tcagactctt cagacgacct tattttcttc acggtcaatc
     7201 cattggataa attttctgta tagtggatta aatttaaacc agtacggcgt tgcctcgcct
     7261 tgccgtacta tctgtactgt ctgcggcttc gtcgctttgt cctgatttaa atttaatcca
     7321 ctatatttta acgcaaattt atttaaaaaa atagacttta aagaaagaaa atcaatcaca
     7381 tagaatttct gcaaacaaag aaaaaaggaa gccgcagctt ccttttcaat tttttggatt
     7441 aagcagccaa agcagatttg gctttttcaa ccaattgtgc aaaagcggct ttatcgaaca
     7501 cggccaaatc agccaatact ttgcggtcga tttcaataga ggcgcgtttc agaccgttca
     7561 taaatttgct gtaagacaac ccgttttcac gcgcacctgc attgatacgg acaatccaca
     7621 attgacggaa ttggcgtttg cgttggcggc ggtcgcggta tgcgtattgg ccagctttca
     7681 ttaccgcctg cttggcaacg cggtaaacgt ttttacgacg accgcggtag cctttggcta
     7741 acgcgaagat tttttggtga cgggcacggg cggtaacacc gcgttttacg cgtggcatat
     7801 tctaaactcc ttaagcgtag ggtaacattt tagcaacaga agccaaatcg cgatcattta
     7861 ccatagaggt accgcgcagt tggcgtttgt ttttggtggt ctttttagtc aagatgtggc
     7921 gtttgaacgc atgagcgcgt ttcacaccgc cgttacccag tactttaaag cgttttttcg
     7981 cgctagactt ggttttcatt ttaggcatgg gaaaactcca ttcgttatcg gataaggcat
     8041 tagggggttt taaaacatcg gtttcaaaca cttgaaccca caatgccacg gttttttgcc
     8101 gcaattaaaa acttactccg aagcggcgat cggagtaagc ggagaattat agctttattt
     8161 tttcttcggc gcaatcatca tcaccatttg gcgaccttcc attttgggaa aggactcgat
     8221 ttgcgccact tcagccaaat cttcttttac acgttccaaa agttgcgcgc cgagttgctg
     8281 gtgagccatt tcacggccgc gaaaacgcaa tgtcactttg actttatcgc cgtcggcaag
     8341 gaagcggtta atgttgcgca tcttgatttg ataatcgccc tcatccgtac ccggacggaa
     8401 tttgatttcc ttgatttgca cctgcttttg atttttcttg gcttcgtcgc gcttcttggc
     8461 ttgttggtat ttgtatttac cgtaatccat cagtttgcac acaggcggtt tagcagttgg
     8521 ggaaatctct accaaatcga catcctgccc ttcggccata gccaaagctt cacgaactga
     8581 aacgacacca agctgttcgc ctgactcact gattaaacgc acttctttgg cggtaatttc
     8641 gccgttgatt cgtgcttcgc gttcttgagc gatgatgaat actcctataa aaattaatga
     8701 ttgacgaggg catcagtgat ttcttgctgc aattgcgcaa tgaaatcatc caaatccaaa
     8761 gaacccaaat cttctgcttt gcggcgtacc gccactttgt tttcctgctt ttctttatcg
     8821 ccgacaacga tttgataagg aaaacggtat tggctgttgt cgcggatttt gtaaccgatt
     8881 ttttcgttac gcaaatccaa ctcggcgcgg aatcctgccg cctgcaattt ggcagccact
     8941 tcacggcagt aatccgcttg attttcggtg atattcataa tcaccatttg aaccggcgcc
     9001 agccataacg ggaaagagcc tgcatggttc tcaatcagaa tgccgataaa ccgctccaaa
     9061 gaacccaaaa tggcgcggtg caacattaca gggcgagcgc ggtcattgtt ttcggttaca
     9121 tattctgcat ccaagcgttc cggtaagacg aaatctagtt gcaatgtacc gcattgccaa
     9181 gaacgaccca aggcatcttt gacgtgatat tcgattttag gcccgtaaaa cgcaccttcg
     9241 ccaggcaact cttcccactc tacgccacaa gcggtcaatg catcacgcaa gccttgttcg
     9301 gccttatccc aaacttcgtc agaacctgca cgttgttcag ggcggaggga caacttcaca
     9361 gcaacatcat ggaaaccgaa ctgtttgtag atgcgaacca acaattcatt gaatgcgcgc
     9421 gcttcgctga cgatttggtc ttcggtacag aaaatatgcg catcatcttg cacaaaaccg
     9481 cgaacacgca tcagaccatg caatgcaccg cttggttcat tgcggtggca agaaccgaac
     9541 tcggccaaac gcatcggcaa atcgcgatat gagcgcaaac cgttattaaa aatttgcaca
     9601 tgacccggac agttcatcgg tttcaccgcg tattcgcgtt tttctgaact ggtcacgaac
     9661 atattgtctt tgtagttgtc ccaatggccg gatttttccc aaaaggtttt atccataatt
     9721 tgaggcgttt tgacctcttt ataaccggcg gcgttcagct ctttacgcat atgctgttca
     9781 atcacttgcc acaaagccca gcctttagga tgccaaaaca ccatgcccgg cgcttcgtct
     9841 tgcagatgga acagatccaa ttgcttgcca agtttgcggt ggtcgcgctt ttcggcttct
     9901 tcgatacgtt gaatataggc ttttaattcg tcttttgtcg cccaagccgt accgtatatg
     9961 cgttgcagca tttcattatt gctgtcgccg cgccagtatg cgcccgccaa cttggtcagt
    10021 ttgaagtttt tcaggaaacg ggtattcgga acgtgcggtc cgcggcacat atcgacatat
    10081 tcctgatgat gatacatccc catcgcttcc acttcgggca tatcgtcaat caggcgcagt
    10141 ttgtattctt cgccgcgctc ttgaaaaatt ttaatcgcct ccgcacgcgg agtcatgatt
    10201 ttgaccacat catagtcttg ggcaatcaat tctttcatac gcgcttcaat ggcggcaaca
    10261 tcttccggtg taaacggttt ttccgtggcg atgtcgtaat aaaagccctc ttcaatgacg
    10321 gggccgataa ccatttttgc attaggatag agttgcttga cggcatgccc gacaagatgg
    10381 gcgcaggaat ggcggatgat ttcgatgcct tcctgatctt tcggagtaat gatttgaaca
    10441 gcagaatttt caacaatcgg gtcgcacgca tcgaccaatt tgccgtttac cctgcctgcc
    10501 accgccgccc tcgccaaacc gacaccgata gacgcagcaa tttgagccac ggtaacgggg
    10561 gattcgtatt ggcggactga gccgtccggc aaggtaatgt tcaacatcaa acgctccgaa
    10621 agattaaaaa ataacggcat aaatgccgtt atgggaattt ggtaggcacg attggattcg
    10681 aaccaacgac ccccaccatg tcaaggtggt gctctaacca actgagctac gtgccttcaa
    10741 agaatttttg cattttatcg ggtcgtttta atttttgcaa gaggtatgtg catttcttta
    10801 tgcaacattg tggcaagatt tggggatgtc gtctgaaaaa ccgtgggaat tcatttataa
    10861 tgccgtcttt taaaacatat ttcgacccct gtcgcccaac tgacattcaa tcttatgctt
    10921 aaatttacct tacacaaaaa agacggatac gcccgacgcg gcacgctgga gttgaaccac
    10981 ggcaaaatcg aaacgccggt atttatgcct gtcggtactt acggctcggt taaggcgatg
    11041 aacccgcaaa acctgcacga catcaaggcg caaattattt tgggcaacac ttatcatttg
    11101 tggctgcgtc cgggcttgga ggtcgtcgaa cagtttggag ggcttcacgg ctttatcggc
    11161 tgggacaaac cgattctgac cgactcgggc ggttttcagg ttttttcttt gtcggatatg
    11221 cgcaagctga cggaagaagg ctgtacgttc aaaagcccga tcaacggcga caagctgttt
    11281 ttatcgcctg aaatttccat gaaaatccaa acggttttga actcggacat cgcgatgcag
    11341 ctggacgaat gcacgccggg cgaagcaacg cgcgaacagg cgaaaaaatc gctgcaaatg
    11401 agcctgcgct gggcggaacg gagcaaaaaa gcctttgaag atttgaaaaa cccgaacgcg
    11461 ctgttcggca tcgtgcaagg cgcgatgtat gaggatttgc gcgaagaatc gctgcgcggt
    11521 ttggaacagt ttgattttcc cggccttgcc gtcggcggtt tgtccgtcgg cgaacccaag
    11581 cccgaaatgt accgtatgct gcgcgccgtc ggtccgattc tgccggaaca caagccgcat
    11641 tacctgatgg gcgtaggcac gccggaagac ctcgtgtacg gcgtggcaca cggcatcgat
    11701 atgttcgact gcgtgatgcc cacccgcaat gcacgcaacg gctggctgtt tacccgtttc
    11761 ggcgatttga aaatcaaaaa cgccaaacac aagctcgaca agcgtccgat agacgaaagc
    11821 tgcacctgct acgcctgcca aaacttcagc cgcgcctacc tgcaccatct gcaccgcacc
    11881 ggcgaaatct tgggcgcaca gctcaacacc atccacaatc tgcatttcta ccaagtcatt
    11941 atggcggaaa tgcgcgaagc cgtcgaacaa ggcaaatttg ccgactggca ggcgcgattc
    12001 cacgaaaacc gcgcacgcgg cacagactga ttccctgcaa tgccgtctga agccgcttca
    12061 gacggcattt tgccgcttta ccctacccgc actatttttc agaaaccgat catgcttcca
    12121 ttctttccag aaccctccct ttcctatacg caacaaaacc gtaccgccgt tttgctgttg
    12181 aacctcggca cgcccgacgc gccgaccgcg caggcggtac gaccttatct gaagtccttt
    12241 ctgaccgacc ggcgcgtcgt cgaactgccc aaatggctgt ggtatcccat tctgcatggc
    12301 ctggtactga cgctccgccc caaaaaaagc gcgcacgcct atgaaaaaat ctggtttaag
    12361 gaaggttcgc ccttagaagt ttacacagca cgccaagctg ccgcgcttgc caaacgtatg
    12421 cccgacctca tcgtccgcca cgccatgacc tacggcaatc cttcggtggc agacgtattg
    12481 tcggaactga aggcgcaagg cgcgggcaga ctgctggtca tcccgatgta cccgcaatat
    12541 gccgcttcca gcagcggagc ggcagtagat aaggtgtgcg aacaattact gctgcaacgg
    12601 aaccaaatga gcgtccgcac cgtttcgcgc ttttatgacg ataccggcta cattgatgcg
    12661 atgaaaaacc acatcctccg atattgggcg gaacacgggc gcgggaaaaa actgatgttg
    12721 agttttcacg gcgtaccgca gaagcaccac gacctcggcg acccctatcc cgacgagtgc
    12781 cgccacaccg ccaaactgct tgccgaagca ctggaactga ccgaagacca atatgtcgta
    12841 tcgttccaaa gccaattcgg cagggcaaaa tgggttacac cgagcacgca ggatttgttc
    12901 ggcaaactgc ccaaacaggg ggtaaccgag ctggacgtat tttgcccggg ctttttggca
    12961 gactgtttgg aaaccatgga agaaatcgcc ctgatggggc gggaacagtt ttacgaagca
    13021 ggcggcaaaa gctaccgcta catcccctgc ctcaacgaca accccgactg gatagacgca
    13081 ctcgtcgcac ttgccgaaga aaaccttggc ggctggcgtt gaaactgttt gacccatgtc
    13141 taaaatgccg tctgaacgcc gtgttcagac ggcattttcc cgctacttta gcgtatgatg
    13201 gcatctatgc cggacaacaa catcaagtgc aaccttacta ccgattcggc aaaaaaatct
    13261 ttcacccgct acgcaaaatc aacaacaagg tttcaaacat taaggaaaca ccaatgaaca
    13321 caacccgact gccgaccgcc ctcgtcttgg gctgcctctg cgccgccgct tctgccgccg
    13381 acaacagcat catgacaaaa gggcaaaaag tgtacgaatc caactgcgtc gcctgccacg
    13441 gcaaaaaggg cgaaggccgc ggaaccatgt ttccgccgct ctaccgctcc gacttcatca
    13501 tgaaaaaacc gcaggtgctg ctgcacagca tggtcaaagg catcaacggt acaatcaaag
    13561 tcaacggcaa aacctacaac ggattcatgc ccgcaaccgc catcagcgat gcggacattg
    13621 ccgccgtcgc cacttatatc atgaacgcct ttgacaacgg cggcggaagc gttaccgaaa
    13681 aagacgtaaa acaggcaaaa ggcaaaaaaa actaaacaga caaaatgccg tctgaaaccg
    13741 gcaatccggc ttcagacggc attcaaatca aacctttcta acgcggtact ttcagcccca
    13801 tagacccgat attgccgtcc ccgtcaacct ccttcgcggt cagcataaag ccgccgatgt
    13861 caatacggcc gcactcgacc ggctcctgac tgtcggaacg cttatcgaaa agctcggcaa
    13921 ggctcaaacc ctcttcgccc gcttccagcc tcaaaccgta agcaagtgcc aaatcacccg
    13981 aacgtgctgc cggcaaaacg acaaactcac cgaagaaatc aaaatactca cggacgctaa
    14041 tacccgtctc ggtaaagtat ttcgccattt tatagtgaa
//
LOCUS       DAWGJV010000042        13754 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWGJV010000042 DAWGJV010000000
VERSION     DAWGJV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.042"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(113..1438)
                     /locus_tag="WHQ94_000897"
     CDS             complement(113..1438)
                     /locus_tag="WHQ94_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ2128205.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1649..2488
                     /locus_tag="WHQ94_000898"
     CDS             1649..2488
                     /locus_tag="WHQ94_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ2128206.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIIVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2485..3651
                     /locus_tag="WHQ94_000899"
     CDS             2485..3651
                     /locus_tag="WHQ94_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ2128207.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3915..5180
                     /gene="efeB"
                     /locus_tag="WHQ94_000900"
     CDS             3915..5180
                     /gene="efeB"
                     /locus_tag="WHQ94_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ2128208.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5252..5581)
                     /locus_tag="WHQ94_000901"
     CDS             complement(5252..5581)
                     /locus_tag="WHQ94_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ2128209.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5658..7028)
                     /gene="glmU"
                     /locus_tag="WHQ94_000902"
     CDS             complement(5658..7028)
                     /gene="glmU"
                     /locus_tag="WHQ94_000902"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ2128210.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNQIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(7083..8063)
                     /locus_tag="WHQ94_000903"
     CDS             complement(7083..8063)
                     /locus_tag="WHQ94_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ2128211.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPPKIRKNVMRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGT
                     PVYYQDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            8122..9123
                     /locus_tag="WHQ94_000904"
     CDS             8122..9123
                     /locus_tag="WHQ94_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2128212.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIAHFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVAINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9217..10065
                     /locus_tag="WHQ94_000905"
     CDS             9217..10065
                     /locus_tag="WHQ94_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ2128213.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10091..10576
                     /locus_tag="WHQ94_000906"
     CDS             10091..10576
                     /locus_tag="WHQ94_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ2128214.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10678..12246)
                     /gene="msrAB"
                     /locus_tag="WHQ94_000907"
     CDS             complement(10678..12246)
                     /gene="msrAB"
                     /locus_tag="WHQ94_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ2128215.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFGSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12391..13656
                     /gene="ftsY"
                     /locus_tag="WHQ94_000908"
     CDS             12391..13656
                     /gene="ftsY"
                     /locus_tag="WHQ94_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ2128216.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKDALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAHGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGESIDDLRPFDARAFVDALLD"
BASE COUNT         2950 a         3524 c         4113 g         3167 t
ORIGIN      
        1 cgtcaaataa gaaaggcatt ttgtccagcc ggcttgtacc ggcggtatcg caaacttcgc
       61 cttaatgcgc ccgattggcc gtgatgggct tcagatggca ttgttttccg gtttacgggc
      121 ggtattcggg cttcataccg ttgggcagaa gctgccagac atatcccgtg gttttctgtt
      181 tgccggcaag ttcgccggct tcgtcgccgt atccccaaaa ataatccacg cgcaccgcgc
      241 ctttaatcgc gctgccggta tcctgcgcca taatcaggcg gttgagggct ttgcgggtaa
      301 ccggatgggc ggtggcgaca aataagggcg cgcccaaggt aatgtagtgc cggtcgactg
      361 cgccggcata ttcccccatc aacggcgtgc ccagtgcgcc gacgggaccg tcattgctgc
      421 ttccggcaag ctcgcggaaa aagatatagc tggggttttg acccaaaact tcggcgaggc
      481 gttgcggatt ttgccgcata taagacttaa tgccctgcat ggaggtttgt ccgagtttga
      541 ggtagccctt atccgccatg tattttccga tggaaacata cggatgctcg tttttgtcgg
      601 catagccgat gcggatgtat ttgccggacg gggttttcag acggcccgag ccttggatgt
      661 gcataaaaaa aagttcgaca gggtcttcgg cgtaaccgag tatcggggct ttgccgtcaa
      721 gcgcgccgcc gttgatttgg ttgcgcgtgt ggtaggggag gaagcggctt ccttcaaacc
      781 tgcctttgat tgccgttgtg cgcgcggtga tggggaatcg ggagaggtcg gcggtatgtg
      841 tgccgccggt attgtcgatt gtgccgctgt ttttccccgt ctgcctgatg cggacaaggg
      901 cttttccgct ccgcaaaccg gcaggcaggg ggacggagat aaaatcgtcg ggaataccgt
      961 aaatcgggaa gcgggcttgt gccgtccgcc tgtcgtcgcc cttcagcacc ggttcgtaat
     1021 agccggtaac cgtaccggca aggcttccgt tgcctgcaac ctgccacggc gtgaaatagc
     1081 gttcaaaaaa ctgttttgcc tgaaaggaat ggacgggggt ttgaaaggct tgggcgcaca
     1141 catcctgcca gccttggcgg tttttcaaat tggcgcagcc gaggcggaag gattgcaggc
     1201 ttttggcgaa atcctgcgcc gcccagtggg gcagggacag gtgcggtaca acggtataga
     1261 cggccccgcc gccgccgacc gtcgttccgg cggggtcggg gatgccgacc ggccggtccg
     1321 ggccgttgat gacggatgtg tcgggttgcg gaaaggtttg gatgctcttg ctttggcagg
     1381 cggcgaggat ggcggcggcg atgccgtaca gggcggcgcg gaataggtat tttttcataa
     1441 tggacaatgt tgccggcagt aataagaaag atggtttcgg gcggcgttgc ggcagccgtg
     1501 gagaggggat tttaacacag ggcgcagctg cagcctgcgg aactttccgc tgcgcggtac
     1561 tgcagataaa aataacttgc atttgtattt acaagcaatg aaaatattcc gataatatat
     1621 tattcatcat ccttgttcgt tcgcgtttat gctggtcgct tttttaatta tgttgcgcga
     1681 gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt tttctgaaac agtccggaca
     1741 ttccaaactg atgcctaagg tctggttcgg ggttgtcctt gcttctttga tgtgtttggg
     1801 gctggggtac ggaatccatt cggcaacagg cgagattccc caaaagcagc aggagttcgt
     1861 cgtcggcatt atcggtttgg ttgctgtcgc gatgctgact tatatgattt tatggatgaa
     1921 aaaagccgcc cgttcgatga agcggcagct tcaggattct gtgcaggcgg ctttgaaccg
     1981 tggcagcggt caaggatggg ccttggtcgg tatggcgttt cttgccgtgg cgcgcgaagg
     2041 tttggagagc gtttttttcc tgcttgccgt attcaaacag agcccgacgt ggcagatgcc
     2101 ggccggtgcg gtagcggggg ttttggctgc cgccgtgatt ggcgcgttga tttatcaggg
     2161 tgggatgcgc ctgaatctgg cgaagttttt ccgttggaca ggggcgtttc tgattatcgt
     2221 tgccgctggt ctgcttgccg gttcgctgcg tgcgctgcac gaagcaggca tttggaacgc
     2281 gcttcaggac attgtgttcg actcatcaaa atatttgcac gaagacagcc cgttgggcgt
     2341 gctgctcggc ggatttttcg gctataccga ccatccgacg cagggcgagg ccttggtttg
     2401 gctgctgtac cttattcccg tcataacttg gtttttgtgc ggcagcaggc cgtctgaaac
     2461 tttaacccgt aaagaggagc tgaaatgaga aaattcaatt tgaccgcatt gtccgtgatg
     2521 cttgccttag gtttgaccgc gtgccagccg ccggaggcgg agaaagctgc gccggccgcg
     2581 tccggtgaga cccaatccgc caacgagggc ggttcggtca gtatcgccgt caacgacaat
     2641 gcctgcgaac cgatggaact gaccgtgccg agcggacagg ttgtgttcaa tattaaaaac
     2701 aacagcggcc gcaagctcga atgggaaatc ctgaaaggcg tgatggtggt ggacgagcgc
     2761 gaaaacatcg cccccggact ttccgataaa atgaccgtca ccctgttgcc gggcgaatac
     2821 gaaatgactt gcggtctttt gaccaatccg cgcggcaagc tggtggtaac cgacagcggc
     2881 tttaaagaca ccgccaacga agcggatttg gaaaaactgt cccaaccgct cgccgactat
     2941 aaagcctacg ttcaaggcga ggttaaagag ctggtggcga aaaccaaaac ttttaccgaa
     3001 gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt ttgccgacac ccgcgtccat
     3061 tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac tcgaccccgt catcgatgcg
     3121 cgtgaagacg acttcaaaga cggcgcgaaa gatgccggat ttaccggctt tcaccgtatc
     3181 gaatacgccc tttgggtgga aaaagacgtg tccggcgtga aggaaattgc agcgaaactg
     3241 atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat tggcgtttcc tccgggcaag
     3301 gtggtcggcg gcgcatccga actgattgaa gaagtggcgg gcagtaaaat cagcggcgaa
     3361 gaagaccggt acagccacac cgatttgagc gatttccaag ccaatgtgga cggatcgaaa
     3421 aaaatcgtcg atttgttccg tccgctgatc gaggccaaaa acaaagcctt gttggaaaaa
     3481 accgatacca acttcaaaca ggtcaacgaa attctggcga aataccggac taaagacggt
     3541 tttgaaacct acgacaagct gggcgaagcc gaccgcaaag cgttacaggc ctctattaac
     3601 gcgcttgccg aagaccttgc ccaacttcgc ggtatactcg gcttgaaata agccgcaagc
     3661 gttcagacgg tatttggcgg cagataccgt ctgaagtttt atagtggatt aacaaaaacc
     3721 agtacggcgt tgcctcgcct tgccttgccg tgctatttat actgtctgcg gcttcgtcgc
     3781 cttgtcctga tttttgttaa tccactatat ccgccatata ttgcagggcg ggatttcaac
     3841 ctgccgctat cggttaatgg aaaaacggcg tgcagggata cccatcctgc tgcacggata
     3901 ttgaaggaaa caccatgagc aaaaaccaac ccgcacaacc gaccaggcgc actcttttta
     3961 aaaccgcgat cgcagccgga gcagtcggcg caatcggagg ttatctcggc ggcaaaaaac
     4021 agggcgaaac cgccgaacgc accgccgaaa gccaacactc gccccaagcc tatccctgct
     4081 acggcgaaca tcaggcaggc atcgttacgc cgcagcaggc gttttcgatt atgtgcgcct
     4141 tcgacgtaac cgcgcaaagt gccaagcagc tggaaaacct gttccgcacg ctgaccgccc
     4201 gcatcgagtt tctcacccaa ggcggcgaat accaagacgg cgacgacaaa cttccgccag
     4261 ccggcagcgg cattttgggc aaagccttca accccgacgg gttgaccgtt accgtggggg
     4321 tgggcagcag cctgtttgac ggccggttcg gactcaaaga caaaaaaccg attcatttgc
     4381 aggaaatgcg cgacttctcc aacgataagc tgcaaaaaag ctggtgcgac ggcgatttga
     4441 gcctgcaaat ctgtgccttc acccccgaaa cctgccaagc cgccctgcgc gacatcatca
     4501 aacacaccgt ccaaaccgcc gttatccgtt ggagtatcga cgggtggcag cccaaatccg
     4561 aacccggcgc gatggcggcg cgcaacctgt tgggcttcag ggacggcacg ggcaacccca
     4621 aagtttccga tcccaaaact gccgacgagg ttttgtggac gggtgtggcc gccaacagcc
     4681 tcgacgaacc ggagtgggcg aaaaacggca gctatcaggc agtccgcctt atccgccact
     4741 ttgtcgagtt ttgggacagg acgccgcttc aagagcaaac cgacattttc gggcggcgca
     4801 aatacagcgg tgcgccgatg gacggcaaaa aagaagccga ccaaccggat tttgccaaag
     4861 accccgaggg tgatatcacg cccaaagaca gccatatacg cctggcgaat ccgcgcgatc
     4921 ccgaattcct caaaaaacac cgcctcttcc gccgcgccta cagctattcg cgcggactcg
     4981 cctcaagcgg acagcttgat gtcgggctgg tgttcgtctg ctatcaggca aaccttgccg
     5041 acggattcat cttcgtgcaa aacctcctca acggcgaacc gctggaagaa tacatcagcc
     5101 ccttcggtgg cggctatttc ttcgtcttgc ccggcgtgga aaaaggcggc tttttggggc
     5161 aagggctgct gggcgtataa atccgccata taaaaaacgc cgtccgaacc ttgccaaacg
     5221 ggttcggacg gcgtttcttg tttttgggcg gtcaggcttt tttgacgaat tcggatttta
     5281 aattcatcgc gctgccgtcg attttgcagc cgatgttgtg atcgccttct tgcaggcgta
     5341 tgcctttgac ttttgtgcct tgtttgatca ccatcgagct gccttttacc ttgaggtctt
     5401 tgatgaggat gacggtatcg ccgttttgca gcactgcgcc gttggcatcg agcacttgag
     5461 ccgcaaggtc ggcggcggat tcggtttcat tccattcgtg ggcgcattcg gggcagatgt
     5521 attgtccgcc gtcttcataa gtgtattcgg aggcgcattg tgggcatggg ggtaatgaca
     5581 tggtttgcag tccttatctg atatcggagg agtttgatgc cgtctgaaac ctgaaaccgg
     5641 cttcagacgg catagcttta ttgtttgttt ttttcaggcc tcacccagcc gtcgatgatg
     5701 gtttggcggg agcgggcgag ggcgagtttg ccgtcttcga cattgcgggt aatcgtgctg
     5761 cccgcgccgg tggttacttt gttgccgatt ttgacggggg agaccaagac gcagtttgaa
     5821 ccgatgcgca cttcgtcgcc gatgacggtt ttgtgtttgt gcacgccgtc gtagttggca
     5881 ataatcgtac ctgcgccgaa gttggttttg ctgccgactt cggcatcgcc gatgtaggtg
     5941 aggtggttgg ctttggtgcc tttgccgatg gcggcgtttt tgatttcgac gaagttgccg
     6001 acgtgtacgt cgttagctag tttggcttgc ggacgcaaac gggcgtatgg gccgatttgg
     6061 ttgttttcgc cgacttcgca gtcttcgagg tgggagaagg gggcgatttt gctgtttgcg
     6121 ccgattttgg cgtttttgat gacgcagttt gcgccgattt cgacgttgtc gccgatttcg
     6181 acttcgcctt caaagataca gttcacatca attacgacat cctgtccgtg tttcagacgg
     6241 cctcgtaaat cgaaacgtgc cggatcgcgc agggttacgc ctgctttgag caattcttgt
     6301 gcctgttcgg tttggaagat gcgttcgagt tcggcgagct ggcgtttgtt gttcacgccg
     6361 gcggcgaggt gggaggcgcg cactcggacg ggatgaactt taataccgtc ggcaacggct
     6421 ttggcgatga ggtcggttaa ataatattcg ccttgggcat tgttgctgga aaggctgttc
     6481 agccagtttt ccagtttggc gttgggcagg acgagaatgc ctgtgttggt ttctttcacg
     6541 gctttttggg cggcacttgc gtctttttct tcgacaatgg cggttacgct gccgctgccg
     6601 tcgcggatga tgcgccccaa acctgacggg tcggcgggca cgtcggtcaa cagcccgact
     6661 tcgttgcctg cggcttcgag cagggtttcg agggtttcaa cgtcaattaa aggaacgtcg
     6721 ccgtacaaca ccagcgtgcg gccttcggcg gcaaggtggg gcagggcggt tttgacggcg
     6781 tggccggtgc cgagctgttc ggtttgttca acccagacga catcgcgttt gacggtgtcc
     6841 aaaacttgct ctttgccgtg gccgacgacg acgcagatgt tttgcggatt cagcgcggcg
     6901 gcggtgtcga taacgcgccc gagcattgtt tcgccgccga tttcgtgcaa aaccttgggc
     6961 attttggaat acatgcgcgt gcctttgccg gcggcgagga tgacgatgtt taaagtgttt
     7021 tgcggcatag cgttttcctg tgcaatgccg tctgaagcgg cttcagacgg catagggtgg
     7081 gtttatcggt tttgaaattt gggcttttgc cagtgttggc gatgctcttc gtcggcgttg
     7141 ttgccggttt ggttgggtaa cacggcatgg cgttcgggac ggtattggtt gtagttcata
     7201 tttttcgagt agctgccgtc ttggtaataa acgggcgtgc cggcgggata tttttgacgg
     7261 acggcggtct tgccgttgcc gtcttgataa gtttcccacg cgcagcccga caaaagggcg
     7321 gcggcagtca tcaggataag gaaggtttta cgcataacat tctttcgtat tttcgggggg
     7381 ggtgtgcggg tattgtaatg attttggcgg tgttctgaca aagtttctgc ataccgagcc
     7441 agttgcgcca tatcgcttac ggaagcatcg ataaagggca ggtcgtgcac ttttgcaccg
     7501 aaccggacgg ttttcatgcc caatgctttt gcctgatgca ggttgtccgc gctgtcgtcc
     7561 accataatgc agcattcggg cggtacgtcc aaaaggcggc agacattgag atacgcttgc
     7621 ggattgggtt tgtacagcag cccgaaatca tccgtgccga acagtgcgtc gaaacggttt
     7681 tccaaaccga gtgcgccgac aacggcacgg acgtaaaacg acgggccgtt ggaaaaaacc
     7741 gccttgcgcc cgttcagacg gcatagggtg ttttccgtat ccgccatgcc gtgcagcctg
     7801 gtcaggattg catcgatcgg atggctttcg cgcaaaaatt cggcgatgtc gatttcggga
     7861 tggtggattt gcagtccggc gagcgttgcg ccgtagcggt gccaatagtc ttgacgcagg
     7921 tcggacgcgg cagattcgga gagtttgagg cggcgtgcca tatagcgtgt catagcgcgg
     7981 ttgatgagtg tgaagaggcc ggcatcggca ttgtgcagcg tgttgtcgag gtcgaacagc
     8041 cacacggtcg ggttttcttg cattttgaac cgtgaaaatt tgttagaatg ttattttaca
     8101 gcgaatagag gaggactcgg aatgaaacgg aaaatttggc tgctgccgct gctggcggtt
     8161 tcggcatacc tgcaggcgca gacggaagtc aggctggcgg tgcataagtc gttcagcctg
     8221 cccaaagggc tgattgcgca tttcgagcgg gcaaacgatg cgaaggtgtc gattattcag
     8281 gcgggcggcg cgaacgaaat gctcaacaaa ctgattttga gccgcgccaa cccgattgcc
     8341 gacgctgtgt atggtttgga caacgccaat atcggcaagg cgcgggaaat gggcattttg
     8401 gcggcggcgc aacccgaatc cgcccccgtc gcggtcgggc tgccttcggc tttggcggtc
     8461 gattacggct atgtggctat caattacgac aaaaaatggt ttgaaggcaa aaagctgccc
     8521 ctgccgcaaa ccctgcagga tttgacccgc cccgaatata aaaacctatt ggtcgtaccg
     8581 tctcccgcca cgtcgtcccc ggggctgggc ttcctgatgg cgaacatcag cggtctgggc
     8641 gaagaaggcg cgttcaaatg gtgggcacag atgcggcaga acggcgtgaa ggtcgccaaa
     8701 ggctggagcg aggcgtatta caccgacttt tcgcacaacg gcggcgcgta tccgctggtg
     8761 gtcggttatg ccgccagtcc ggcggcggaa gtgtattttt ccaaaggcaa atacagcgag
     8821 ccgccgacgg gcaacctgtt tttaaaaggc ggcgtattcc gccaggtcga aggcgcggca
     8881 gtcttgaagg gcgcgaaaca gccgaagctg gcggcaaaac tggtgcaatg gctgcaaagt
     8941 cgggaagtgc agcaggcggt tccgtccgaa atgtgggttt accccgccgt caaaaacacg
     9001 cgcctgcccg acgtgttccg cttcgcccaa gccccgacgc acaccaccgc ccccgcgcag
     9061 cgcgatattg atgcgaacca gcgcggatgg gtttcccgtt ggattagaac ggttttgaaa
     9121 taaaacaaac ataccgcccc gcagggcttc atacggcatt tttacacctg tgccgattac
     9181 gccgcatggg gcggatgttc gatcaagagg aaaacaatgg acttcaaaca atttgatttt
     9241 ttacacctga tcagtgtttc cggttgggag catctggctg aaaaggcgtg ggcgttcggg
     9301 ctgaaccttg ccgccgcgct gcttattttt ttggtcggaa aatgggcggc gaaacgcatt
     9361 gtcgctgtga tgagggcggc gatgacgcgc gcgcaggtcg atgccacgct gattagtttt
     9421 ttgtgtaatg ttgccaatat cggcttattg attttggtga ttattgccgc attgggcaga
     9481 ttgggcgttt ccacaacatc cgtaaccgcc ttaatcggcg gcgcgggttt ggcggtggcg
     9541 ttgtccctga aagaccagct gtccaatttt gccgccggcg cgctgattat cctgttccgc
     9601 ccgttcaaag tcggcgattt tatccgcgtc ggcggttttg aaggatatgt ccgagagatt
     9661 aaaatggtgc agacttcttt gcggacgacc gacaacgaag aagtcgtgct gcccaacagc
     9721 gtggtgatgg gcaacagcat cgtcaaccgt tccacactgc cgctgtgccg cgcccaagtg
     9781 atagtcggcg tcgattacaa ctgcgatttg aaagtggcga aagaggcggt gttgaaagcc
     9841 gccgtcgaac accccttgag cgttcaaaac gaagagcggc aggccgccgc ctacatcacc
     9901 gccttgggcg acaatgccat cgaaatcaca ttatgggctt gggcaaacga agcagaccgc
     9961 tggacgctgc aatgcgactt gaacgaacaa gtggtcgaaa acctccgcaa agtcaatatc
    10021 aacatcccgt tcccgcaacg cgacatacac atcatcaatt cttaaacgcc gtctgaaaga
    10081 ggagtgggaa atggacgcgc tgcacaccat cgcccgaaac ctgacgaaaa aacgtcaaac
    10141 cgtaagctgt gccgaatcct gcacgggtgg gctgcttgcc gccgtattca caagcgttgc
    10201 aggcagttcg caatggttcg atcaaagttt tgtaacatac agcaacaaag ccaaagaaga
    10261 ccgcttgggc gtgttgcccg aaaccctgct cgaacacggc gcggtcagcc gccaaaccgt
    10321 ctatgagatg gcgcgcggcg cgaaagccgt ggcgcaggcg gattacgccg tcggtatttc
    10381 cggcatcgcc ggtccgggcg gcggcagcga aagcaaaccc gtcggcacgg tttggttcgg
    10441 gtttgccttt ccgggcggaa gttgcgaagc aatgcgccgt tttgacggca accgcgaatc
    10501 cgtccgcgcg caggcggtcg ccttcgcgtt ggaacggttg gcggggctga ttgaaaacgg
    10561 cggcgatgcc gtctaaacaa aatctccgtc tggacaaaat ccccgccgga taaaaaatac
    10621 cgtctgaaac gttgcaggtt tcagacggta ttttgtcggg ttaggcggcg gtgcggctta
    10681 tttcacttta ctcttcaacg cgccgtagcc tgccgcgtcc atttgttcca gcgggatgaa
    10741 tttcaagctc gcgccgttga tgcagtagcg cagtccgcct ttgtcgcgcg ggccgtcggg
    10801 gaagacgtgc cccaagtgtg agtcggcggc gtggctgcgc acttcggtgc ggcgcatgtt
    10861 gtagctgaaa tcatcgtgtt cggtaacgga ttttgcatca atcgggcgcg tgaagctcgg
    10921 ccagccgcag ccggaatcat atttgtcggc ggagctgaac aaaggttcgc cgctgacaac
    10981 gtccacataa atgccgggtt tgaacaagtg gtcgtattcg tggctgaagg catattcggt
    11041 cgcgctgttt tgggtaactt ggtattgctc ttcggtcagg gtgcgtttga gttcggcgtc
    11101 actcggtttt ttatacgttg ccgcatcgaa gcctttgcct tgcggggcgg tcttggtttt
    11161 gcccggcagc ggttcgtcgg ctttgcggat atcgatgtgg cagtagccgt tggggttttt
    11221 aatcaggtag tcctgatggt attcctcggc atcgtagaag tttttcagcg gctcgttttc
    11281 aacaacgagg ggcagttggt atttttgctg ctcgcgtttg agggcggcgg cgatgacggc
    11341 tttttcggcg gggtcggtgt agtacacgcc gctgcggtat tgcgtaccgg tgtcgttgcc
    11401 ctgtttgttg aggctggtcg gatcaacgac gcggaagaaa tattgcagga tgtcgtctag
    11461 gctgagtttg tcggcatcgt aggtcacttt gacggtttcg gcgtggcccg tatggcggta
    11521 ggacacgtct tcatagctcg gatttttcgt gttgccgttg gcgtagccgg ataccgcgtc
    11581 aaccacgccg tcgatgcgtt ggaaataggc ttccaagccc cagaagcagc cgccggcgag
    11641 gtagatggtg cgcgtgttca tgatttttga atcctttttc tgagtgtcgg gtttgtagaa
    11701 cgaatgtttc aaactgccca aatcggcatt cgggtcgcgg attaacgcca acgcctgcgc
    11761 ttcgttgatg ctgcctttga cgatgcgctg cacgtcgccg tctttaccga ttaacgccca
    11821 cgaagggtaa acgctgatat tcaggctttg ggcgatcgtg ccgccgttgt cggttacgac
    11881 gggcagcttg ggataattca aaccggcata ccatttttgg aagtcgccgt cttttttctc
    11941 gtgcaaaaag cccggagacg cgacggtaat caggttggcg gaaccgaatt ttgcatcttg
    12001 cgcccatttt tcggtctgtc ccaattcgga cagacacaaa ggacaccagc tcgcccaaaa
    12061 tttaatcagc gtcggtttgt cttttttcaa gtaaacactg gcggggcggt tgtccgcagt
    12121 tttcaaagtg gataaagtgt gcggcacggt cgcggctccg gcatcgacga ttttgggcga
    12181 acaagcaccc agcgcaagca ggcagccgaa cttggcgcaa agggaaaaga aagtacggtg
    12241 tttcattttg agtttcctgt gtggacggtt tgcatgatta gacgtttgag atgccgaaac
    12301 cttacagctc ggattttcag acaaccttgc cgcgtaaaat acgctacaat actacctatt
    12361 tcaagtttct aaaattaaaa ggaaaattca atgttcagct tcttccgtcg caagaaaaaa
    12421 caggaaacgc cggctctcga ggaggctcaa attcaggaaa ccgcagcaaa agcagagtcc
    12481 gaaaccgatc aagtaattga aaatattaaa gaagatgctg aatctttagc agaaagcgtc
    12541 aaagggcagg tcgaatctgc cgttgaaacc gtcagcggtg cggttgaaca ggtaaaggaa
    12601 accgttgccg agatgctgtc tgaagcagag gaagcggcgg aaaaagcagc ggaacaagtc
    12661 gaagcggcaa aagaagccat tgccgaaacc gtcggcgagg ctgtcgggca agttcaagaa
    12721 gccgttgcga caactgaaga acacaagctc ggttgggcgg cgcgtttgaa acaaggtttg
    12781 accaaatcgc gcgacaaaat ggcgaaatcg ctggcgggcg tgttcggcgg cggacaaatc
    12841 gacgaagatt tgtacgaaga gctggaaacc gtgctgatta ccagcgatat gggcatggaa
    12901 gccaccgaat acctgatgaa agacgtgcgc gaccgcgtca gcctcaaagg gctgaaagac
    12961 ggcaacgaat tgcgcggcgc gctgaaagat gcgctgtacg acctgattaa gcctctggag
    13021 aaacctttgg tcttgcccga aaccaaagag cctttcgtga ttatgcttgc aggtatcaac
    13081 ggcgcgggca aaaccacgtc tatcggcaaa ctcgccaaat atttccaagc gcatggaaaa
    13141 tccgtattgc tggcggcagg cgatactttc cgtgccgccg cgcgtgagca gcttcaagct
    13201 tggggcgagc gcaacaacgt aaccgtgatt tcgcaaacca cgggcgattc cgccgccgtg
    13261 tgcttcgatg ccgtccaagc cgccaaagcg cgcggcatcg acatcgtgct tgccgacacc
    13321 gccggccgcc tgcccacgca gcttcatttg atggaagaaa tcaaaaaagt gaaacgcgtg
    13381 ctgcaaaaag ccatgcccga cgcgccgcac gaaatcatcg tcgtgcttga tgccaatatc
    13441 gggcaaaacg ccgtcaacca agtcaaagcc tttgacgacg cattagggct gaccggttta
    13501 atcgtgacca aactcgacgg cacggcaaaa ggcggcatcc tcgccgcgct tgcctccgac
    13561 cgccccgtcc ccgtccgcta catcggcgtg ggcgaaagca tagacgacct gcgcccgttt
    13621 gacgcgcgcg cgtttgtgga cgcactgctg gattgagccg aaatgccgtc cgaaaacagc
    13681 agaccgatgc cgtcattccc gcgcaggcgg gaatccagac cttgggataa cggcaatatt
    13741 caaagtttat aaaa
//
LOCUS       DAWGJV010000043        13587 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWGJV010000043 DAWGJV010000000
VERSION     DAWGJV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.043"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            244..5694
                     /gene="iga"
                     /locus_tag="WHQ94_000909"
     CDS             244..5694
                     /gene="iga"
                     /locus_tag="WHQ94_000909"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ2128217.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWNITSG
                     TNSKIGSTAVRLANNERNANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGAN
                     NDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTDRASTITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRK
                     PILQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWVFMGYKQEEAQKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFGATQINGN
                     VNLSQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGA
                     SLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQ
                     IGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQK
                     ANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTL
                     TAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQKYSAGAALL
                     YRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5872..7074)
                     /gene="trpB"
                     /locus_tag="WHQ94_000910"
     CDS             complement(5872..7074)
                     /gene="trpB"
                     /locus_tag="WHQ94_000910"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ2128218.1"
                     /translation="MKNYHAPDEKGVFGEHGGLYVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7259..7948)
                     /locus_tag="WHQ94_000911"
     CDS             complement(7259..7948)
                     /locus_tag="WHQ94_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ2128219.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8145..8924
                     /gene="rsmA"
                     /locus_tag="WHQ94_000912"
     CDS             8145..8924
                     /gene="rsmA"
                     /locus_tag="WHQ94_000912"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ2128220.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVDINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9097..9825
                     /locus_tag="WHQ94_000913"
     CDS             9097..9825
                     /locus_tag="WHQ94_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128221.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9898..10257
                     /locus_tag="WHQ94_000914"
     CDS             9898..10257
                     /locus_tag="WHQ94_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128222.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            10262..10702
                     /locus_tag="WHQ94_000915"
     CDS             10262..10702
                     /locus_tag="WHQ94_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ2128223.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            10734..12008
                     /gene="folC"
                     /locus_tag="WHQ94_000916"
     CDS             10734..12008
                     /gene="folC"
                     /locus_tag="WHQ94_000916"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ2128224.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDMPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12021..13019
                     /gene="ftsN"
                     /locus_tag="WHQ94_000917"
     CDS             12021..13019
                     /gene="ftsN"
                     /locus_tag="WHQ94_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ2128225.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            13012..13509
                     /locus_tag="WHQ94_000918"
     CDS             13012..13509
                     /locus_tag="WHQ94_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ2128226.1"
                     /translation="MNSLPIADLLVSAVIAACIVISTMRGVITEAGSMAAWVVSFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         3938 a         3571 c         3288 g         2790 t
ORIGIN      
        1 cacaagcggg aatccaaaaa caaaaagccg cagaaattta acggaagaac cgaaccccga
       61 aaaccggctt cccgccttcg cttgaataac agaacaaagg aagcggaaat aaaaacatcg
      121 ggattctttt tttaaattgg caataaaaca ccaaaatgaa tgatttacac aacaaaaatt
      181 agtcaacacc gccaaaccgg cgtaaaatac aaaacattat cacccttaaa acggtaaaac
      241 cttatgaaaa ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
      301 gcccttacgc catactcaga agcagcattg gtcagagacg atgtcgatta tcaaatattc
      361 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
      421 aacaaacaag gtcaaaacat cggcaacgca ttatccaacg taccgatgat tgattttagc
      481 gttgcagatg tcaacagacg tacattaacc gtcattgatc cccagtatgc cgtcagcgtc
      541 aaacatgtaa aaggagacga aatctcttat tatgggcatc acaatgggca cttagatgtt
      601 agcaatgatg aaaacgaata tcgttcagtt gcacaaaatg actacgaacc aaataaaaat
      661 tggcatcacg gtaatcaagg tcgtcttgaa gactacaaca tggcacgcct taataaattc
      721 gtaacagaag tcgcacctat tgcaccaacc agtgcaggcg gcggcgttga aacctataaa
      781 gataaaaacc gtttttctga gtttgtgcga gttggagcag gtacgcaatt tgaatataac
      841 agccgctaca atatgacaga gctttcacga gcttatcgtt atgccattgc aggtacgcct
      901 tatcaagacg tgaatgtcac atcgaatcta aatcaagaag gcttgattgg cttcggtgat
      961 aattcaaaac atcattcccc agaaaaactc aaagaagttt tatctcaaaa cgcattgact
     1021 aactatgctg tgttaggcga tagcggttca ccattatttg cttatgataa acaagaaaaa
     1081 cgctgggtct ttttaggtgc ttacgattat tgggcaggct atcaaaaaaa ctcttggcaa
     1141 gaatggaata tctataaaaa agaatttgca gatgaaatca aacaacgcga taacgccggc
     1201 accatcaaag gttatggcga acatcattgg aacattactt ctggcacaaa cagcaagatt
     1261 ggttcgacag cggtaaggct tgccaacaat gaaaggaatg caaacaacgg acagaatgtt
     1321 acctttgaaa acaacggcac tttggtattg gatcaaaaca tcaaccaagg cgcgggcggt
     1381 ctgtttttca aaggcgatta cacagtcaaa ggtgcaaata atgacatcac ttggctaggt
     1441 gcggggattg atgttgccga cggcaaaaaa gtcgtttggc aagtcaaaaa tccgaatggc
     1501 gacagattgg caaaaatcgg caaaggcaca ttagaaataa acggcacagg cgttaaccaa
     1561 gggcaattaa aagtcggcga cggtacggtt attctgaatc aacaagccga tgccgacaaa
     1621 aaagtccagg ctttctccca agtcggcatt gtgagcggac gcggtacatt ggtgctaaat
     1681 agttcaaatc agattaatcc cgataaccta tatttcggtt tccgtggcgg tcgtttggat
     1741 gccaatggca atgacttgac ttttgaacac atccgcaacg tggatgaagg cgcgcgcatt
     1801 gtcaaccaca acacagaccg cgcctccaca atcacattaa ccggcaaatc attaattacc
     1861 gctcctcaaa atctttctgt ttatgaaatc cgaaatgatt acgatgatga cgattattat
     1921 ggctattaca gctatagaaa acccattctg caaggcaaag atctttatta caaaaactat
     1981 cgttattacg ccctaaaatc cggcggcagc gtgaacgcac cgatgcccga aaacggacaa
     2041 acggaaaata acgactgggt ctttatggga tataagcaag aagaagccca gaaaaacgcg
     2101 atgaaccaca aaaacaatca gcgtattagc ggttttagcg gtttctttgg cgaagaaaac
     2161 ggaaaaggac ataacggcgc attaaacctt aatttcaacg gcaaaagcgc gcaaaaccgt
     2221 ttcttgttaa caggcggcac gaatttaaac ggaaaaataa gcgtaactca aggcaatgtc
     2281 ttgttatcag gtcgtccaac accacacgca agagattttg tgaacaaatc ttcagcccga
     2341 aaagacgcac atttctccaa aaacaatgaa gtcgtatttg aagacgactg gataaaccgc
     2401 acattcaaag ccacagaaat tgcggttaat caatccgcat cattctcttc cggcagaaat
     2461 gtatccaaca ttaccgccaa catcaccgca acagacaacg ccaaagtaaa tttgggttac
     2521 aaaaacggcg atgaagtttg cgtgcgctcg gactataccg gttacgttac ctgcaacaca
     2581 gacaacttat ccgataaggc tttaaacagc tttggagcga cacagattaa cggcaatgtg
     2641 aatttgagtc agaatgcggc attggtctta ggcaaggctg cattatgggg gcaaattcaa
     2701 gggcaaggaa acagcagcgt cagcctaaac caacatagca aatggcattt gaccggcgac
     2761 agccaagtac acaatctgtc attggcggat agccatattc atttgaacaa cgcttccgac
     2821 gcgcaaagtg caaataaata ccacacgctc aaaatcaatc atttatccgg taacgggcat
     2881 tttcattatc tgacgcacct ggcgaaaaat cttggggata aggtgcttgt gaaggaatcc
     2941 gcatccggtc attatcagct ccatgttcaa gataaaacag gcgaacccaa tcaggaaggg
     3001 ctgaatctct ttgatgcatc atccgtacaa gaccgttcgc acctttctgt ttccttggcg
     3061 aataaccatg tagatttagg cgcattgcgt tatacaatca aaacagaaaa cggtattacc
     3121 cgattgtaca atccttatgc cgagaaccgc cgccgagtca aaccggcccc gtctcctgcc
     3181 acaaacacgg cttctcaagc acaaaaggca acacaaacgg acggtgcaca aattgccaag
     3241 cctcaaaata tcgtcgtcgc accacctagc ccgcaggcaa atcaagccga agaagcaaaa
     3301 cgccaacaag caaaagcgga gcaagtgaag cgtcagcaag cagaagcaga gcgtaaatcg
     3361 gcagagttag caaaacaaaa agcagaagcc gaacgcgaag caagagagct ggcgacccgc
     3421 caaaaagcag aacaagaacg cagcagcgcc gaacttgcac gtcgacatga aaaagaacgc
     3481 gaagcggcag agttgtcggc aaaacaaaaa gtcgaagccg aacgtgaggc tcaagcattg
     3541 gcggtacgcc gaaaagccga agccgaagag gcaaaacgcc aagctgcaga gcttgcacgc
     3601 cggcatgaaa aagaacgcga agcggcagag ttgtcggcaa aacaaagggt cggggaagag
     3661 gaacgccgtc aaacagccca atcccagccg caacgccgca aacgccgtgc cgcaccgcag
     3721 gattatatgg cagcttccca agaccgtcct aaacgccgcg gacaccgttc ggtacagcaa
     3781 aacaatgtgg aaattgccca agcccaagca gaacttgtgc gccgacagca ggaagaacgc
     3841 aaagcggcag agttgttggc aaaacaaagg gcagaagccg aacgtgaggc tcaagcattg
     3901 gctgcgcgcc gaaaagcaga agccgaagag gcaaaacgcc aagctgccga acttgcacat
     3961 cgccaggaag cagaacgcaa agcggcagaa ttgtcggcaa accaaaaagc cgcagctgaa
     4021 gcccaagcat tggcggcacg ccaacaaaaa gcccttgcgc gtcaacagga agaagcacga
     4081 aaagctgcag agttagccgt caaacaaaaa gcggaaaccg aacgcaaaac cgccgaactt
     4141 gccaagcaaa gggcagcagc cgaagcagca aaacgccagc aagaagctcg ccagaccgcc
     4201 gaacttgcac gtcgtcaaga agcggaacgc caagcagcag agttgtcggc aaaacaaaaa
     4261 gccgaaacag atcgagaagc ggcagaatcg gcgaaacgaa aagctgagga agaagagcac
     4321 cgtcaagcag cccaatccca gccgcaacgc cgaaaacgcc gtgccgcacc tcaggattat
     4381 atggcagctt cccaaaatcg tccgaaacgt cgcggacgca gatctactct gccggcaccg
     4441 ccctcgccat catttgattc gtcagcttac gcagcaccca gggccttgca taatccggac
     4501 tggtatgaaa atgactatga agaaatcccc ttggatgcgt tggaagatga aaatgtatct
     4561 gaatcagttg acacatcaga caaacagcct caagacaata cggaacttca tgaaaaatat
     4621 gaaaatgatt atgaagaaat ccccttggat gcgttggaag atgaagatgt atccgaatcg
     4681 gttgacacat cagacaaaca gcctcaagac aatacggaac ttcatgaaaa agttgagaca
     4741 gtgagtttgc aaccaagagc cgcgcagccg cgagcccaag ccgccactca gccgcaagcc
     4801 caagccgccg cgcaagccga tgcagtcagc accaatacca actcggcttt atctgacgca
     4861 atggcaagca cgcaatctat cttgttggat acaggtgctt cattaacacg gcacattgca
     4921 caaaaatcac gcgctgatgc cgaaaaaaac agcgtttgga tgtcgaacat cggttatggc
     4981 cgtgattatg cttccgcaca atatcgccgg tttagttcga aacgcacgca aacacaaatc
     5041 ggcattgacc gcagcttgtc cgaaaatatg cagataggcg gcgtattgac ttactctgac
     5101 agtcagcata cttttgatca ggcgagcggc aaaaatactt ttgtgcaagc caacctttat
     5161 ggtaagtatt atttaaatga tgcttggtat gtggccggcg atattggtgc aggcagcttg
     5221 agaagccggt tacaaacaca gcaaaaagca aactttaacc gaacaagtat ccaaaccggc
     5281 cttactttgg gcaatacgct gaaaatcaat caattcgaga ttgtccccag tgcgggaatc
     5341 cgttacagcc gcctgtcatc tgcagattac aagttgggta acgacagtgt taaagtaagt
     5401 tctatgtcag tgaaaacact gacggccgga ctggattttg cttatcggtt taaagtcggc
     5461 aaccttaccg taaaaccctt gttatctgca gcttactttg ccaattatgg caaaggcggc
     5521 gtgaatgtgg gcggtaattc cttcgcctat aaagcagata atcaacagaa atattcagca
     5581 ggtgccgcgt tactgtaccg taatgttaca ttaaacgtaa atggcagtat tacaaaaggc
     5641 aaacaattgg aaaaacaaaa atccggacaa attaaaatac agattcgttt ctaaaatact
     5701 aaattcatag caaaataaaa tgccgtctga actcaggctt cagacggcat ttttatagtg
     5761 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag acggcaaggc
     5821 gaggcaacgc cgtactggtt tttgttaatc cactatatca gacggacgag gttacagttt
     5881 aatccctttg agcttcgcga cggtattgat gtctttgtcg ccacgacctg agaggttgac
     5941 cagaatcact tggtctttac ccattttcgg cgcatttttc accgcccaag caacggcgtg
     6001 gctggattcg agcgcgggga tgatgccctc gaagcggcag agcaagtcaa aggcttcgag
     6061 tgcttcgtcg tctttggcaa cggtgtattc gacgcgcttg atgtcgtgca gatggctgtg
     6121 ttccgggccg atgccggggt aatccaagcc tgcggaaaca gagtgtgtac ccaagacttg
     6181 accgttttcg tcctgcatca gatagctgcg gaaaccgtgc aatacgccaa tcggtgagcc
     6241 cgaagtaatc ggcgcggcgt gatcgggggt gttcacgccc aaaccgccag cctccacgcc
     6301 gacgaggcgc acgttttctt cgccgatata cgggtggaac aaaccaatgg cgttcgatcc
     6361 gccgcccacg caggcaacgg caacgtcggg ctgtctgccg atggcttcca acatttgttc
     6421 tttggcttca ttgccgatca cgcattgaaa gtcgcgcacc atttcgggat acggcgcggg
     6481 accggcggcg gtaccgatga tgtagaatgt gtcatccacg cgggcgaccc attcgcgcat
     6541 ggcttcgttc atcgcgtctt tcaaagtacg gctgccgctg tcaacgccga cgacattcgc
     6601 gcccaataat ttcatacgga acacgttggg catttggcgt tggatgtcgt ccgcacccat
     6661 gtacacgtcg caagtcatgc cgaagcgggc ggcaacagtg gcactcgcca cgccgtgctg
     6721 acccgcgccg gtttcggcga tgacgcgttt tttacccata cgttttgcca acagtgcctg
     6781 accgatggtg ttgttgactt tgtgcgcgcc ggtgtggttc aagtcttcgc gcttcaacca
     6841 aatttgcgcg ccacccaaat gctcggacaa tcgcgcggca tggtaaacgg ggctgggcct
     6901 gccgacgtag tgtttcaaat cgcggtggaa ttcttcccaa aacgacgggt cgtttttcgc
     6961 tgccttatag gcatccgcca gctcttgcaa ggcgggaatc agggtctcgg aaacataaag
     7021 cccaccgtgt tcgccgaaaa cgcccttctc gtcgggtgcg tggtaattct tcatcagatt
     7081 tcgtccttgt gcttcaaatt ttcagacggc aatttatagt ggattaacaa aaaccggtac
     7141 ggcgttgccc cgccttgccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga
     7201 tttttgttaa tccactatat ttttcaaaca tgccgtccga acgggaaatt gtcggcaatt
     7261 ataactgctt ttcacacaag cccgccattt caaaaaacgc ccgatacgcc gccgcttttt
     7321 tcgccaaact caaaggatag gcacgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc
     7381 gtccggcaaa cggaaacggc acggtttcgc ccgttttcgg cattttgatg ccgccaccct
     7441 gaatatcgag caggatttcc gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg
     7501 gtatttttgc caacaccgcc gccaaatcga cggtttcaac tacctttaaa aacttgtcgg
     7561 acgcattgcc gtgttccctt gccgccttca acacggtcgg acaggacgca attccccgtt
     7621 cgcgcaaaaa cgccttgatt ttctcggcat caaacgcttt ttcagacaac ctttggaaat
     7681 gcgccgcatc attaaaaaac accagcccgt aaacgcgcca catatcgttt tggaaattcg
     7741 gataatgaaa ctgcatcgcg cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga
     7801 ccgcagcctt cggcggcaac acggaatcga agggatgggt ttcgatttca agcagatttt
     7861 cagacacgtt caatcctcca acaggcaaaa cggcagacag ccggtttttg caccaaaccg
     7921 ccaaacacgg caagccttca aacagcatta tcacgccctg ccgttttcgg ttacaatgcc
     7981 gacatcaaac ggatactgta aacacattta tgttccaaca cacaggacga cacttaaagc
     8041 accgccctat gtgccatcct gatttggaag gggttacgcc cctcccaaat agagtctgat
     8101 tctaccgccc taaagggcgg ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag
     8161 cccgcaagcg tttcgggcag aattttttgc aggacacgcg gattatcagc gatattgtca
     8221 acgccgtgcg cccgcaggcg gatgatgtcg tgattgaaat cggtccgggt ttggcggcga
     8281 ttaccgaacc tttggcgaaa aagctgaacc gcctgcacgt tgtcgaaatc gaccgcgaca
     8341 tcgtatgccg tctgaaaacg ctgccgtttg cggacaagct cgtcattcat gaaggcgacg
     8401 tgttgcagtt tgatttcaac ggcatcgcag gcaaaaagaa aatcgtcggc aacctgccgt
     8461 acaacatttc cacgccgctt ttgttcaagc tggcggaggt ggcggacgat gtcgtcgata
     8521 tgcactttat gctgcaaaaa gaagtggtcg agcgtatggt tgccgcgccg aaaagcaacg
     8581 actacggccg cttgggcgtg atgttgcaat atttcttcga tatggaaatg ctgattgacg
     8641 tgccgcccga atcgttcgac cccgcgccga aagtcgattc cgccgtcgtc cgcatgattc
     8701 cggtgaaaca ccgcatcggc agggcggacg atttcgagta tttcgccaaa ctcgtgaaac
     8761 tcgccttcca ccaacgccgc aaaaccatac gcaacaatct gaaagagctt gcaggcgacg
     8821 atgatttgca ggcagtcgac atcaatccgc aagaccgtgc cgaacatatc gcgccggaga
     8881 aatatgtggc gttgagcaat tatctagcgg gcaaggccgt ctgaaaaggg agtaagccga
     8941 tgacgaaata cctgctttta cccctgatac tgccgcttgc agcctgcctg accaatccga
     9001 acgaccgttt ttttgacggc agacgctaca cgcttcatca tatggagctc ccgtaacgac
     9061 aacctgcaac ggcaaatgcc gtctgaaaga aaagacatga tcaaattcaa aaacgtacac
     9121 aaacatttca aagacctgca cgtcatcaac ggcgtgaact tggaaatcaa aaaaggcgaa
     9181 gtggtcgtcg tctgcggacc ttcgggcagc ggcaaatcca cactcatccg caccgtcaac
     9241 cagcttgaaa gcatcgaaag cggcgagatt tgggtggacg gtgtcaacgt tgccgaccca
     9301 aaaaccgatt tgaacaaaat ccgcgaagaa gtcggctttg tgtttcaaag tttcaacctc
     9361 tacccgcacc tgaccgtgtt ggaaaacatt accctcgcac cgatgaaggt taaaggtcaa
     9421 aatgcggaac aggcagagaa aaaagcgatg gaacttttgg aacgcgtcgg actggcacac
     9481 aaaaaagacg ccttcccttc ccaactttcc ggcggccagc agcaacgcgt ggcgattgca
     9541 cgcggtttgg cgatggaacc gcgcgtaatg ttgtttgacg aaccgacctc cgcactcgac
     9601 cccgaaatgg tcggcgaagt gttgaaagtg atgaaagact tggcggaaag cggtatgacg
     9661 atgatgtgcg taacccacga aatgggtttt gcccgcgaag ttgccgaccg agtgcttttt
     9721 gtcgataaag ggcaaatcct cgaagacgaa acgcccgaag cgttttttac caacccgaaa
     9781 cacgaacgcg ccaaacaatt cctgcaacag gtcatgaccc actgatgcta tgccgtctga
     9841 aaaccgaacg ttcagacggc atagcccgac accgcaagat gattgagaaa gaaaaccatg
     9901 tattttgttg accgcaccgc cgtcgtcctc aagccgaccg tccgcttttt ggaatggctc
     9961 aaaagcaccg acgaaaatat gcccgacctg accatagaac agcttcgtgc caactgttcc
    10021 gtcttcctcg ttccgcagtt cgacgaaccg gaagccgtcg tttcctattt cgacgaacgc
    10081 taccgccaga ttttcgaggc ggagctggca ggttgggaca tcgataagga taaatggccg
    10141 caagatatgg ggctgaaggc gttttgggaa tttttcgaca tcgaaatcca cgatatggtg
    10201 ttggacatgg aagaagcgga attgaacatt acccccgtgt tcgacaacat gatgtaaggc
    10261 tgtgcgcgcc tttcagacgg cattgaggcc gtcccgcatc ctgaacatcc tgactgtatc
    10321 gcttcacgcc gcctcccttg ccgtctgcct gacttggttt tacggctgga tgatgtggtt
    10381 cggtttggcg gctttggtgg caagttacgc atactcgctc aggataacga atctgaaaca
    10441 ccgccatgcc ataaccgcca ttaccataga ccgcgacgga caggcggaaa tcgtatccgg
    10501 caaagacaaa acagcggcaa cacttgcggg cagcagtatg gttacgcctt atgccttatt
    10561 cttacaatgg gacacaggcg gcacaaccgt cagccaatgc atcatacccg acatgacgga
    10621 caaagaatcc taccggcggc tgaaagtctg gatactttgg cggcagccga aaaaaaccgc
    10681 cgaaaccgat acaccaagct gatttcagac ggcatttgaa cacggaacac tcaatgaaaa
    10741 cactgcaaga ctggctctcg catttggaaa ccgcccacag cggcggcttg atcgatatgg
    10801 gcttggaacg cgtaggcgaa gtgaaaaaac ggatgaagct cacgccgcaa tgccccgtcg
    10861 tcgttgtcgc gggaacaaac ggcaaaggtt cggtctgcgc ctacctgaca cagatttaca
    10921 aacaggcggg ctacaaaacc ggcacactga ccagcccgca tttattgcgt tacaacgaac
    10981 gcatcgccat caacgccgaa cccgtttcgg acgatacgat tattgcctct ttcgagcgca
    11041 tcgaagcggc gcgcagcgaa atatcgctga cttattttga attcaatacg ttggcggccg
    11101 tcgacatctt catgcgcgaa caggttgacg tgatgatatt ggaagtcggc ttgggcggac
    11161 gcttggacgc ggtcaacgcg tttgacggcg attgcgcggt ggttaccagc gtggatttgg
    11221 accatcaggc atttttgggc gatacggtcg agcaggtcgg ctttgaaaaa gcaggcgtgt
    11281 tccgtagcgg caaacccgcc atctgcggtc aaaaccccgc gcccaaatcg ttgctttcac
    11341 acgccgaagc cataggcgcg aaactgctga tggtgcagcg cgatttcgag tttcacgcgc
    11401 tggaaaacat ccaatggaac taccgcttcc gaccgcagca ttcagacagc cccgctcgca
    11461 accgtaattc cctgcccttc cccgcattgc gcggcgcata ccaactttcc aatgccgcct
    11521 gtgcgctgac cgtgttggaa tgtttgaacg acaaattgcc ggtggacatc ggtgcaatca
    11581 aacgcggatt gctgctggtt gaaaatcccg gacgcttcca agtcctgccc ggccggccgc
    11641 tgaccgtttt ggacgtcgga cacaatcccc acgctgcccg cgccctgcgc cgcagcctga
    11701 ttaatttggc gtttgcgcaa aaacgcaccg ccgtgttcag tatgctgtcc gacaaagaca
    11761 tagacggcgt gttggaaatt gtcaaagacc agtttgacga atggtatatc gcacctttgg
    11821 acatgccgcg cggcatgacg ctcgagagcc tgcaaaacaa actgcacgaa cacagcatcg
    11881 aaaacataca gacattcgcc gccgtccgcg aagcataccg ggcggcagcc tcaaaagcgg
    11941 gcgagaacga cagaatcgtc gttttcggct catttcatac cgttgccgat gtcatgtcgg
    12001 tactttaaaa ggaaacttct atgtccgaaa acaaacaaaa cgaagtcctg agcggttacg
    12061 aacaactcaa acggcgcaac cgccgccgcc tcgtaacggc aagttgcttg gttgccgcct
    12121 cctgcatcct gctggcagcc gccctcagtt ccggccctgc cgaacagact gccggcgaaa
    12181 caagcggcgt agaaaacaaa gcggcaggtg cggcacaaac ccctgccttg aaatccgccg
    12241 ccgacaaacc gcaggacttg gcaggcgaag acaagccttc tgccgccgac agcgaaatca
    12301 gcgagcctga aaacgtaggc gcgccgctgg tgctgattaa cgagcgcctc gaagacagca
    12361 acatcaaagg tttggaagca tccgagaaac tgcaacaggc agaaaccgcc aaaaccgcac
    12421 cgaagcaggc aaaacaacgc gctgccgaaa aagtgccggc aactgccgac agtacggata
    12481 cggtagcggt tgaaaaaccg aaacgcactg ccgaaacaaa accgcaaaaa gcggaacgca
    12541 ctgccaaagc caagcccaaa gccaaagaaa ccaaaaccgc cgaaaaagtt gccgacaaac
    12601 cgaaaactgc cgccgaaaaa accaaaccgg atacggcaaa atccgacagc gcggtaaaag
    12661 aagcgaaaaa agccgacaag gctgaaagca aaaaaacagc cgaaaaagac cgttcggacg
    12721 gcaaaaaaca cgaaacggca caaaaaaccg acaaagcgga caagaccaaa accgccgaga
    12781 aggaaaaatc cggtaaaaaa gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct
    12841 taagcctcca gcgcaaaatg aaggcggcgg gtatcgattc gaccatcacc gaaattatga
    12901 ccgacaacgg caaagtttac cgcgtcaaat caagcaacta taaaaacgca agggatgccg
    12961 aacgcgattt gaacaaattg cgcgtacacg gtatcgccgg tcaggtaacg aatgaatagc
    13021 ctccccattg ccgacctcct cgtctccgcc gtcatcgccg cctgcatcgt catttccacg
    13081 atgcgcggcg tgattacgga ggcaggctca atggcggcat gggtggtttc cttctttttc
    13141 gccaaactct ttgccgcacc cttcgccgac atcgcctttg catcgttcca accccgcctg
    13201 tttgcattgg ctctgtcgtt catttcccta ttcgtcattg cctgtctgat ccagaaaata
    13261 ctccgctcgc tgctgaccgg ggcagtttcg gcggtcggtt tgggctttgc caaccgcatt
    13321 ttgggcggcg tattcggtgc attgaaaggc attttgatta ttaccctgct ggtcatgctc
    13381 gcttcaaaaa ccgacctgcc cgataccgaa gaatggcggc aatcttacac actgccgttt
    13441 ttcgtatcgc tttccgaagc cgtgttgaac catagcggcg gcacggcgga aactccggaa
    13501 gacgattgat tgccatacgg atatttcaga cggcattctc ttttatgccg tctgaaatgt
    13561 tttttccttt cctcagccat atcacag
//
LOCUS       DAWGJV010000044        13484 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWGJV010000044 DAWGJV010000000
VERSION     DAWGJV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.044"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(143..233)
                     /locus_tag="WHQ94_000919"
     tRNA            complement(143..233)
                     /locus_tag="WHQ94_000919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ser,seq:gga)
     gene            complement(272..358)
                     /locus_tag="WHQ94_000920"
     tRNA            complement(272..358)
                     /locus_tag="WHQ94_000920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Leu,seq:cag)
     gene            complement(394..480)
                     /locus_tag="WHQ94_000921"
     tRNA            complement(394..480)
                     /locus_tag="WHQ94_000921"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(444..446),aa:Leu,seq:cag)
     gene            569..2944
                     /gene="rnr"
                     /locus_tag="WHQ94_000922"
     CDS             569..2944
                     /gene="rnr"
                     /locus_tag="WHQ94_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ2128227.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIEALVRELSIKEEEYEFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGDNNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAVEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3283..4746)
                     /gene="guaB"
                     /locus_tag="WHQ94_000923"
     CDS             complement(3283..4746)
                     /gene="guaB"
                     /locus_tag="WHQ94_000923"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ2128228.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4947..5027)
                     /locus_tag="WHQ94_000924"
                     /pseudo
     CDS             complement(4947..5027)
                     /locus_tag="WHQ94_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5073..5534)
                     /locus_tag="WHQ94_000925"
     CDS             complement(5073..5534)
                     /locus_tag="WHQ94_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128229.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5625..8183)
                     /gene="glnD"
                     /locus_tag="WHQ94_000926"
     CDS             complement(5625..8183)
                     /gene="glnD"
                     /locus_tag="WHQ94_000926"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ2128230.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8231..8551)
                     /locus_tag="WHQ94_000927"
     CDS             complement(8231..8551)
                     /locus_tag="WHQ94_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128231.1"
                     /translation="MMGNKLTPPAKLPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8876..9349)
                     /gene="bfr"
                     /locus_tag="WHQ94_000928"
     CDS             complement(8876..9349)
                     /gene="bfr"
                     /locus_tag="WHQ94_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ2128232.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9377..9841)
                     /gene="bfr"
                     /locus_tag="WHQ94_000929"
     CDS             complement(9377..9841)
                     /gene="bfr"
                     /locus_tag="WHQ94_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ2128233.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10304..11287)
                     /gene="lipA"
                     /locus_tag="WHQ94_000930"
     CDS             complement(10304..11287)
                     /gene="lipA"
                     /locus_tag="WHQ94_000930"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ2128234.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(11280..11903)
                     /gene="lipB"
                     /locus_tag="WHQ94_000931"
     CDS             complement(11280..11903)
                     /gene="lipB"
                     /locus_tag="WHQ94_000931"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ2128235.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11905..12180)
                     /locus_tag="WHQ94_000932"
     CDS             complement(11905..12180)
                     /locus_tag="WHQ94_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ2128236.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12365..13435
                     /locus_tag="WHQ94_000933"
     CDS             12365..13435
                     /locus_tag="WHQ94_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ2128237.1"
                     /translation="MYQRKGWGIKPWMGAGAAFAALVWLIFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIS
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2943 a         3179 c         3813 g         3549 t
ORIGIN      
        1 taccacaaaa cataccataa aaaaacggta acggatagtt gtaagcggtt gatgacgatt
       61 atagacaata acgggttttc ccaatgaaat tattgtttga ataataaaaa atcccaaacc
      121 gtaaaagttt gggatttgta tttggcagag aggaagggat tcgaaccctc gatacgctat
      181 tcacgtatac acgctttcca ggcgtgcgac ttaaaccact catccacctc tctaatggcg
      241 gaaattatcc caatcgggat aatttattat ttggtgcccg ggagaagact cgaacttcca
      301 cacccgtgag gataccagca cctgaagctg gcgcgtctac caattccgcc acccgggcaa
      361 tctaaatatt gaaataaagc aaagcatttg atttggtgcc cgggagaaga ctcgaacttc
      421 cacacccgtg aggatactag cacctgaagc tagcgcgtct accaattccg ccacccgggc
      481 tttgctttat ttgctgtttt gcggtacagt gtcctgccgc aaagaagtcg ctattatata
      541 gttcataaag agaatgtcaa cagtccaaat gaataaaaat attaaatctt taaatttacg
      601 ggaaaaagac ccgtttttaa gtcgtgaaaa acagcgttat gaacatcctt tgcccagtcg
      661 ggaatggata atcgagctgc ttgaacgtaa aggcgtacca tccaagattg aagctttggt
      721 acgcgaattg tcgattaagg aagaagagta cgaatttttc gaacgtcgtc tgaaggcgat
      781 ggcgcgggac ggtcaggttt taatcaaccg tcggggcgcg gtttgcgcgg cggacaaatt
      841 ggatttggtc aaatgccgtg tcgaggcgca caaagacggc ttcggtttcg ccgtgccgct
      901 cacgcccgcc aaagacggtg attttgtttt gtacgaacgc cagatgcgcg gcattatgca
      961 cggcgatatt gtcactgttc gtcctgccgg catggaccgt aggggccgcc gcgaagggac
     1021 ggttctggat attgtcgaac gcgcgcaaag caaagttgtc ggccgtttct atatggatag
     1081 gggcgtggcg attttggagc cggaagacaa gcgtctgaac caaagcatcg tattggaacc
     1141 ggacggcgtg gcgcgtttca aacctgaatc cggtcaggtc atcgtcggcg aaattgaggt
     1201 ttatcctgag caaaaccggc cggcagtggc aaaaatcatc gaagttttgg gcgattatgc
     1261 cgacagcggc atggagattg aaattgccgt gcgcaagcat catttgccgc accaattcag
     1321 tgaagcgtgt gccaaagccg cgaaaaaaat tcccgaccat gtacgcaaaa gcgatttgaa
     1381 aggccgcgtc gatttgcgcg atttgccttt ggtaacgata gacggcgaaa cggcgcgcga
     1441 ttttgacgat gccgtgtttg ccgaaaaaat cggacgcaat taccgtctgg tcgtggcgat
     1501 tgccgatgtc agccattatg tccgccccga tgacgctatc gacacggacg ctcaggaacg
     1561 cagcaccagt gtttacttcc cgcgccgcgt gattcccatg ttgccggaaa acctgtccaa
     1621 cggcatctgc tcgctcaatc ctaatgtcga gcgtttgtgt atggtgtgcg atatggtcat
     1681 cacttacgcg ggcaatatta aagaataccg cttctacccc gccgtgatgc gctctcatgc
     1741 ccgcctgacc tacaaccaag tttggaaatg gctttcagac ggcatcgggc atccgttcaa
     1801 agcacaaatc gacacgcttt acaaactctt caaaatcctt cagaaaaagc gtttcgaacg
     1861 cggggcggtg gagtttgaca gcatcgaaac ccaaatgctt ttcgacgaca acggtaaaat
     1921 tgaaaaaatc gtccccgttg tccgcaacga tgcccacaag ctgattgaag aatgtatgtt
     1981 ggcggcaaac gtttgcgcag cggattttct gttgaaaaac aagcataccg cattgttccg
     2041 caaccatttg gggcccacgc ccgaaaaact cgccgccttg cgcgagcagc tcggtctgtt
     2101 ggggcttcaa cttggcggcg acaacaaccc gtcgccgaaa gactatgccg cgcttgccgg
     2161 acagttcaaa ggcaggccgg atgccgaatt gctgcaagtc atgatgttgc gctccatgca
     2221 acaggcggtt tacgaaccgc attgcgacgg acactttggt cttgcctacg aagcatacgc
     2281 ccacttcacc tcgcccatcc gccgctatcc cgacctgacc gtacaccgcg ccatcaaagc
     2341 cgtgttgaat cagcaaacct acacgccaaa aaaaagctgg caggctttgg gcgtgcatac
     2401 ctcgttctgt gagcgccgtg ccgacgacgc cagccgcgac gtggaaaact ggctgaaaac
     2461 ctattatatg cgcgataagg tcggcgaagt attcgaaggt aaaatctccg gcatgaccag
     2521 ttttggtatc tttgtaacac tggacggcat ccacattgac ggcttggtgc atatcagcga
     2581 tttgggcgaa gactatttca acttccgccc cgaaatcatg gcaatcgaag gcgaacgcag
     2641 cggcatccgt ttcaacatgg gggacagggt tgccgtccgg gtcgcccgtg ccgatttgga
     2701 tgatggaaaa atcgattttg tcctgattgc cgaggggagc ggcagggggc ggaaagttaa
     2761 atcatccgcg tctgccaaac cggcagggac ggcggggaaa gggaaaccga aaaccgccgt
     2821 cgagaaaaaa acagcccgag gcggcaaagt aaggggaagg ggcgcgtctg ccgccgcaga
     2881 atcggggaaa aaggcaaaga aaccggttcc gattaaggta aaaaaacgga aaggcaaatc
     2941 ataatgctga cggggtggct tggggagggc ggggcatact ttcccgaagc cgccgcccga
     3001 ttttttgata tggcggccgg cgcatcattt caggccaatc gaaacgcccg tattgaaaga
     3061 ttgcgtttat ttccaccgcc gttttaaagg ccggcggtat tcggcagacg gggcgcaaac
     3121 ggcgttcaga cggcattttc attctttcgg cgtgtccgtc cgaatggctt tgcccgtccg
     3181 cgcaatcagc ccgtgcggct tgccttgaac ggacaaaaaa tgccgtctga aacccgaaaa
     3241 tcaggtttca gacggcattt tgccttgaaa aggctgttca aatcagcggt ggtagttcgg
     3301 tgcttcttta gtgatttgaa cgtcgtgaac gtgcgattcg ctcatacctg cggaagtgat
     3361 ttccacaaat tcggcttttt cgtgcatttc ggcaatattg gcgcaaccca agtaacccat
     3421 actggaacgc agaccgccgg tcagctggtg gatgatgttc acaatcgggc ctttgtaagg
     3481 aacgcggcct tcgatgcctt cggggacgta tttgtcggtg ctgtcggttt tgtcttggaa
     3541 gtagcggtcg gcagaacctt ggctcatcgc gcccaatgaa cccataccgc gataggattt
     3601 gtacgagcgg ccttgataga gttcgatttc gcccggcgct tcttccgtgc cggcaaacat
     3661 accgccgagc atgacgctgt acgcgcctgc ggcgagggct ttggcgatgt cgccggagaa
     3721 gcggatgccg ccgtcggcaa tcaggggaac gcctgtgcct ttgagggctt cggcaacgtt
     3781 gtggatggcg gtcagttgcg gcacgcccac gcctgccacg atacgggtgg tgcaaatcga
     3841 tcccgggccg ataccgactt tgacggcatc cgcaccggcg gcgaccaaat ccaatgcggc
     3901 tttggcagtg gcgatgttgc cgccgatgac ttggatgtgc ggataggttt ctttcaccca
     3961 tttcacgcgg tcaatcacgc cttggctgtg cccgtgggca gtatcgacga caatcacgtc
     4021 cacgccggct tcgaccaagg ctttgacgcg ctcttcggta tcgccgccgg tgccgactgc
     4081 cgcaccgacg cgcagacggc cttcggagtc tttgttggcg ttgggaaact cggtggtttt
     4141 taaaatatct ttaacggtaa tcagaccttt gagttcgtct ttttcgttca gaaccaaaac
     4201 gcgctcgact ttgtgcgtgt gcatcagttc gcgcgcttcg tctatgcttg tgccttcggg
     4261 gacggtaacc aaacgttcgc gcggggtcat aatggcggaa acgggcaaat cgacgcggtt
     4321 ttcaaaacgc aggtcgcggt tggttacgat gccgacgact ttgccgtttt caacgacggg
     4381 caggccggac attttgcgtt tgcgctgcgc acgcatttcc aagacttcgc ggatgagcgt
     4441 cgtcggtgca acggttacgg ggtctttgac cacgccgctt tcgtggcgtt tcactttgga
     4501 aacggcgcgc gcctgcattt cgggcggcat gtttttatgg atgatgccga tgccgccttc
     4561 ctgtgccatc gaaatggcga ggcgcgcctc ggtaacagtg tccatcgcag cggaaagcag
     4621 ggggaggttg agtgtgattt cgcgggtgag cttggtttga agtttaacgt ctcgcggcag
     4681 cacggtcgaa tgtgcgggaa ccaacaaaac atcgtcgaaa gtataggctt tttctacgat
     4741 acgcataatg ctcggtcttt cagtttgtgc aagatgcacg gcattatagc acgttaccgg
     4801 cggcttgaca gtttatcagg tttaattttg gtcccctttg aatagcccgg ttttcctttg
     4861 ccgaccactg ttgctcccgt tctttcaatt tcaggaaaag cttttttcta atttttggta
     4921 agtggctcag ttattgaagc cctatatcgg gcggtaactt ccagcacaaa aaaacggagt
     4981 agttctttat ttattttttc ctttaatttt cagtatatta tcttaatatt tcgagggtaa
     5041 catatctgct aatctagtta cagccccata tattatagat tcaattgaaa aataacagat
     5101 tcaactgtac ctttcttata cctgatttct ttaaagtttt tcccattgtc aaaactataa
     5161 aaaagtctct cttcctcacc agaatatccc atttctaata ctaaacaatc tatttcacaa
     5221 ttaccccctc cactcatata tgtagtatca atagaaaata tattgcataa ttccagagaa
     5281 ttatatttat ctatatcaaa tagttgctct ccaaaatcta aaccattgtt tccacctgca
     5341 taatacttct cttcaaagga tgacttcaaa ctattaaaaa ctgcatttag cgtaaaatat
     5401 ccatatgggc atatcttagg atatatattc tctcctatta ttatctgaat ttttccataa
     5461 ataaaaccat tacattcaga gttttcttcc cataaaatcc caaattttct tgttgagtcc
     5521 ataataatat tcatataaat ccttatatta ataaattatt tacaatatcc cccgctttca
     5581 gacggcatac ggcgtggcgg cggaatgccg tctgaaggcg ggcgttatga taattgttcc
     5641 agcagcgtct gcttcaatga ggactggatt tttgggtttt tcaggtctag gctaaaaacg
     5701 gtaaaactgt cttcggcgcg ttcatccagt gtggagattt tggcatagcg caggctgacg
     5761 ttgtgggcga aaaagacttc cgccatatcg gcgagcagga aggggcggtt gacggcggtg
     5821 atttcgacgg agtaccaatc ggggtagtct tcttcggggg tgatggtgat gcttggcgcg
     5881 atcggcatat agcggctgcg gcggctgatg cggcggctat ggctggagat ttcggcaacg
     5941 gtgtgtccgt ggataaagct gttgagttcg gcttcgagcg cgctttgaat gtcggggtag
     6001 tcttcgggag cgtgctgcga ggggatttgc acgatgaagg tgtcgaggat gtagtcgtgt
     6061 tcggtgatga aggcgcgggc ggcgagaatg tcgaagccgt ggcggctgaa gatgcggcag
     6121 aggcgggcga acaggcgcgg gccgttgggc ataaagacca tcacttggaa gctgtcgctt
     6181 tggggcagga tgcggctgcg gacgatgggg gtttcaaaat cgtgtacgag gttggcggcg
     6241 tgccacagga tttcgcggga ctggtggcgg gcgaagtagg cggaaccgag cgcgttccat
     6301 agttttttct gctgtttttc ggggacggcg gcgcgggtga gtaagtcggc ggcttcctgc
     6361 cggcggcggc cgaagagggt gtgcgggttg ccgccgttgc ctgtaaggta gcgtccggcg
     6421 gcatggaaga ggctttccag caggctggcg cgccatgcgt tccacagctt gggattggta
     6481 ccgcgtatgt cggaaatggt cagaaggtag agcgcgctga ggcgttcgtg ggtttggacg
     6541 cgtttgcaga aagcatcgag tacgtcgggg tcttggatgt cttctttttg ggcgacggca
     6601 gacatcagca ggtggttttc gaccagccaa gcgagcaggt cgctttcttc tccggtcagg
     6661 aagtggtcag cggcaaattg gcgcgcgtct gcgatgcctt gtatggcatg gtcgccgccg
     6721 cgtcctttgg cgatgtcatg gaaaaaggcg gcaaggtaga ggatgtcttg tttttcaaag
     6781 gactgcatca gtgcagaggc gtagggcagc tcgtggctgt gcatatccaa ggcaaggcgg
     6841 cggacgttgc ggacgacggt gaggatgtgg tcgtccacgg gatagatgtg gaacaggtcg
     6901 tgttggagca ggccgatgat tttttcccac gcgggcaggt agcggcccaa cacgccgtag
     6961 aggttgagaa agcgcagggt ctgggtcagc ccgttgccgt tgcggaaaaa accggcgaag
     7021 cggcggcggt tttcgggatt ttggtagaag ctgcggttga ttttgcgcgt cgcgccccac
     7081 caggcgcgca gggtttgcgg ttcgagcgcg gtaatgtcgt tgcgctgctg catgatttcg
     7141 acgattttga aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg cgcggcgatt
     7201 tggttgttga cttggatgta gtcgtcgtca atccgcaggg taacgcgcaa cggggtggag
     7261 gaaacgcggc tttgcagcat aggcgtgagg atgccgccca gttgtttgac ggttttaatc
     7321 gcgcggtaaa acacgcgcat cagttcttcg ctttggcggc ggaggttcaa gccttcataa
     7381 cccatgcttt cggcgacttg cggctgcaaa tcgaacagca ggcggtcttc ggcgcgattg
     7441 gcgtttaaat gcagatggat gcggatgtgg gcgaggcggc ggtagccgtg cgaaagcata
     7501 ccggcttcgg cacgcgtcaa aatccgctgt ttgagcaggt cgggcaggtc ggtcgccaag
     7561 ccttgcgcct tcgctatcca aagcagggtg tggatgtcgc gcagaccgcc cggacagctt
     7621 ttgatattcg gctccaatac tgcccccgaa ccttgcgatt tggcgtggcg gtgttccatc
     7681 tccaccagtt ttgcctcgac aaacgccgcc acattgcgct gcgcgttcat tttttccgcc
     7741 agtttatcca ccgtttggcg gttgccaaac aaaaacctag cctctaaaaa cgctgtgtcc
     7801 cccgtaatat cattgcgcac gctttcacat agttcatcaa cgctgccgct ttttacagac
     7861 ggcatcagtt tgcagtccca cagggtttga acaaaccggg caatctgttc ctgaatgccg
     7921 tctgaaagcg gggcagggga gacaaccgcc aaatccacat ccgaacaggg atacagttcg
     7981 ccgcgcccga agccgcccac cgccatcagg cataacgcgc tgttttgaaa atgttctacc
     8041 cacaatgccg ccagtaaggt ttcgactgcc gccgtatatt ccctgaaaaa taccgacacg
     8101 cggttggctt tcaaataatg cgcttcggcg gcatcgcgct gccgtttgaa ggtttccagt
     8161 gctgaagaca ggtttgcagg catttttatt ctttcgattg gcgggaaaag ggaggtggat
     8221 ggttcggcgg tcaaataccg ctttcagacg gcatttgtcg ggtatcggcg gaattggtca
     8281 tcagcttcaa ccgttcctgc ggcggcagca gcaactgata cgccgccacg cccaaagccg
     8341 ccgccatttt ttcgatattc gacagggcga tgttccagcg tttgcgctcg actgccgaca
     8401 cataagtcct gtccaaaccg cattgccgcg ccaattcttc ttgcgaccaa cccttgttca
     8461 cgcggaaaag ccgcatattg tatgccaata ccgcccgcaa atcctgttcg tcaggcagtt
     8521 tggcaggcgg ggtcaatttg ttgcccatca tttgcttcca gataaatggt taaagttaag
     8581 catttgctgt tacggatttt acttcacata aaagccaaaa aaaaggggtg gcggcagcca
     8641 cacccaaaca cacacacatc aagaggaaag aggtaaaatc aagacagatt gggaaacagc
     8701 aaacaagcaa tctagatttc atatcggtat tatcaaacaa aaaccgttat cgagtattga
     8761 ttcaaatcaa tttagatttg attcgcaaac cggcaaagaa aaacggcata cccgtttata
     8821 cggatatgcc gtctgatgat gcaggggact gctatatttg gcagtggttt gtgttttagt
     8881 cctcttgcgc cgctgtttgc aggtagttcg gcaaaccaat tttgccgatc agttcctgct
     8941 gggtttcaag ccagtcgatg tgttcttcgt tggtgtcttt ttgtttttcc aacaaatcac
     9001 ggctgacgta atcctgttgc gcttctgctg tggcgatggc ggcaagcagg gcttcgtgtt
     9061 tttcctgttc tttggtcaaa tcgcaggcga tgatttcttc ggtggactcg ccaatcagaa
     9121 gcttgcccag ttcttgcagg ttcggcagac cttcgaggaa cagaatgcgc tcgatcaaat
     9181 cgtcggcagc tttcatttca acgatggact gtttgaagaa atgttcgccc agttcttcaa
     9241 agccccagtt tttcaaaata cgggcgtgaa ggaaatattg gttgatggtt accagcagca
     9301 agcctaagtt tttgtttagc tcgcggataa ccagacggtc gcctttcata cggactcctt
     9361 ttattccgaa tacggattac agttggcttt ggtagtagtt gccttcgcca attaactcga
     9421 tcaggcgaag ctgctgttcc agccagtggg cgtggtcttc ttcggtatct ttcagttggg
     9481 caaccatcag gtcgcgcgta acatagtctt gagcctcttc gcacagtttg atgccttttt
     9541 tcaacgcatc acgtacttca tattcggttt gcaggtcggc tttgaggcag gaaaccacgt
     9601 ccgtgccgat attcagttcg gcgcgtgcca ttttcggcgt gccgcccagc atcaggatgc
     9661 ggcggatgaa gtcttcggcg tgtgtggttt cttcttccat ctcgtggttg agacgttcaa
     9721 aaagtttggt gtagccccat tcggagtaga ggcgggagtg gataaagtat tggtcgcgtg
     9781 ccgccagctc gccagacagc aattcgttca tgtaatctaa aactgcttga ttgccttgca
     9841 taatatttct cttttcttaa tttgggtttc gtgtagttaa acagtggatg tagcactgtt
     9901 cggaatgcgt gcattgtatg caaaagctgt cggcatgaca aattttctat ttaaaataca
     9961 aacaattatc aaaagaaata gggcgtattc cccgatgcct tccaaatcag tatgctgttt
    10021 tttatcggtt ttttgtgctg ttaaaactaa taatccatat tatccataaa gaacatttac
    10081 acttgttaac catagctaaa agcaaatacg gtacggtttt ttatcaaaat ttataatgaa
    10141 tcgttctcat taaccgacag attcttttga gattatcgga ttttgggaat aaattatgcc
    10201 gtctgagggg ctttcagacg gcatagacaa ttaaagctga ttaaacaacg cggcagacag
    10261 cagcgtagga aattattttt tacttcggaa cggtattttg ggtttaatga tgcccgcaac
    10321 cgccgtggct ttcgcgtaag gcttcggcag cctgttcgtc ggcgtggtag ctggaacgta
    10381 ccatcgcgcc gatggcggca ttgctgaagc ccagttcgta tgcttctttt tcaaagattt
    10441 tgaactgctc gggcgtaacg tagcgcagga cgggcaggtg tccgtctgaa ggctggaggt
    10501 actgtccgat ggtaatcatt tcgatattgt gcgcccgcat atcgcgcata atttcacgca
    10561 cgtcttcgtc tgtttcgccc aagccgacca tgatgccgga tttggtcggg atgtgcggca
    10621 tcatttcttt ataacgtttt aataagtcta aagaatgttg ataattggca ccgggacggg
    10681 cttttctgta caggctcgga tgggtttcta ggttgtggtt catcacgtcg ggcggggttt
    10741 cggcaaggat tttgagtgcg atgtccaagc gtcctcggaa gtcggggacg aggatttcga
    10801 ttttggtgtt cgggctggtt tcgcggatgg ctttgatgca gtcggcgaaa tgctgtgcgc
    10861 cgccgtcgcg caggtcgtcg cggtcgacgg aggtgatgac gacgtaacgc aggttcatgg
    10921 ctttgacgga ttcggcgagg tttttcggtt cgtcggggtc gagcatattc gggcggccgt
    10981 gtcccacgtc gcagaacggg cagcggcggg tgcagatgtc gcccataatc atgaaggtcg
    11041 ccgtgccttt gctgaagcat tcgccgatgt tggggcagga ggcttcctcg caaacggtgt
    11101 gcattttttg ttcgcgcaaa atgtctttga tttcaaagaa tttgcgcgat ggaagtttgg
    11161 cgcgtatcca ttcgggcttt tttaattttt cctgaagggg gacgactttg atggggatgc
    11221 gggcggtttt gtccgcgcct ctgagtttga tgccgcgttt ggggtcgtcg gttttgattt
    11281 cactcattgt tgtctgcttt cggtgtgaat tgtgtttcaa ggtgtgcggt aagtttggcg
    11341 gcgacttcgt ccggcgtggg gcagggttgg acaaaatccg cgatttgcgt catttccata
    11401 ccggcgtagc cgcaggggtt gatgtgggta aacgggctta aatccatatt gacgttgagc
    11461 gcaagcccgt gatagacgga gccgtttttg atacgcagcc ccagtgaggc gattttgcgt
    11521 tctccgacat aaacgccggg gcgtttgggg tctgccgccg cttcgatgcc gtattctgcc
    11581 aatgtggcga tgatgctgtt ttcaagcgcg gaaacgatgt tgcggacgct ggttttgcgc
    11641 cgtttgaaat caatcatcgt ataaacgacc aattgcccgg gcccgtgata ggtaatctgc
    11701 ccgccccggt cgatttggac gacgggaatg tcgtcgcgaa tcagcaggtg ttcgggtttt
    11761 cccaccagtc cttgtgtgaa cacgggcggg tgttcgacga cccacagttc gtcttcggtg
    11821 tcagcattcc gtccggcatt aaaggttttc atcgcttcaa aagtcggcag atattcgacc
    11881 aaacctttgt gtatgatttt catctcaaag taccactttg accagttcgt gcgaagtcag
    11941 cgcgcggtag atgttgtcca attgttcttg gttttcaacc tttacctgaa cggtggcgcc
    12001 ggtatagttg cctttgctgc tcggacgcgt ggtgatgtgg tgcgcctgcg tgtcgggggc
    12061 gtggaggcgg acggtgtcca ataccgcctg ctcgaactcg ggatgaaccg cccccattac
    12121 ttttaatggg aaggtgcagg gaaattcgat gagggatgtt ttgttttctt gttcggtcat
    12181 gatgtgctgc cttgttgtgt acggtatgcc gtctgaaggc gggtttgcct ttcagacggc
    12241 atcggatgtg cgttatttta gcctaaaccg cgataacagg ctatcgggaa ggcggaggct
    12301 tttttgacgg cgcggcggtt ctgctatact ggcgcgcaat attattttta gaagggtggt
    12361 ttttatgtat cagaggaaag ggtggggcat caagccgtgg atgggtgccg gtgcggcgtt
    12421 tgccgccttg gtttggctga ttttcgcgct cggcgatact ttgactccgt ttgcggttgc
    12481 ggcggtgctg gcgtatgtgt tggacccttt ggtcgaatgg ttgcagaaaa agggtttgaa
    12541 ccgtgcatcc gcttcgatgt ctgtgatggt gttttccttg attttgttgt tggcattatt
    12601 gttgattatt gtccctatgc tggtcgggca gttcaataat ttggcatcgc gcctacccca
    12661 attgatcagt tttatgcaga acacgctgct gccgtggttg aaaaatacaa tcggcggata
    12721 tatggaaatc gatcaggcat ctattattgc gtggcttcag gcgcatacgg gcgagttgag
    12781 caacgcgctt aaggcgtggt ttcccgtttt gatgaggcag ggcggcaata ttgtcagcag
    12841 tatcggcaac ctactgctgc tgcccttgct gctttattat ttcctgctgg attggcagcg
    12901 gtggtcgtgc ggcatcgcca aactggttcc gaggcgtttt gccggtgctt atacgcgcat
    12961 tacgggtaat ttgaacgagg tattgggcga atttttgcgc gggcagcttc tggtgatgct
    13021 gattatgggt ttggtttacg gcttggggtt ggtgctggtc gggctggatt cggggtttgc
    13081 aatcggtatg gttgccggta ttttggtttt tgttccctat ttgggcgcgt ttacaggact
    13141 gctgctggca accgtcgccg ccttgctcca gttcggttcg tggaacggca tcctatcggt
    13201 ttgggcggtt tttgccgtag gacagtttct cgaaagtttt ttcattacgc cgaaaatcgt
    13261 gggagaccgt atcgggctgt cgccgttttg ggttatcttt tccctgatgg cgttcggaca
    13321 gctgatgggt ttcgtcggaa tgttggcagg attgcctttg gccgccgtaa ccttggtctt
    13381 gcttcgcgag ggcgtgcaga aatattttgc cggcagtttt taccggggca ggtaggcggt
    13441 tccgaaacat atttgaagcg ggatacaacc ctgcccgggt ttaa
//
LOCUS       DAWGJV010000045        13414 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWGJV010000045 DAWGJV010000000
VERSION     DAWGJV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.045"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..246
                     /locus_tag="WHQ94_000934"
     CDS             <1..246
                     /locus_tag="WHQ94_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ2128238.1"
                     /translation="ITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNIS
                     DREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
     gene            663..1379
                     /locus_tag="WHQ94_000935"
     CDS             663..1379
                     /locus_tag="WHQ94_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128239.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1482..1907
                     /locus_tag="WHQ94_000936"
     CDS             1482..1907
                     /locus_tag="WHQ94_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128240.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            2151..2351
                     /locus_tag="WHQ94_000937"
     CDS             2151..2351
                     /locus_tag="WHQ94_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128241.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2419..2778
                     /locus_tag="WHQ94_000938"
     CDS             2419..2778
                     /locus_tag="WHQ94_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128242.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2803..3657
                     /locus_tag="WHQ94_000939"
     CDS             2803..3657
                     /locus_tag="WHQ94_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ2128243.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3727..3942
                     /locus_tag="WHQ94_000940"
     CDS             3727..3942
                     /locus_tag="WHQ94_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128244.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNPKNAA"
     gene            3977..4237
                     /locus_tag="WHQ94_000941"
     CDS             3977..4237
                     /locus_tag="WHQ94_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128245.1"
                     /translation="MKYLIRTALLAVAAVGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            4234..4527
                     /locus_tag="WHQ94_000942"
     CDS             4234..4527
                     /locus_tag="WHQ94_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128246.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4530..4721
                     /locus_tag="WHQ94_000943"
     CDS             4530..4721
                     /locus_tag="WHQ94_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128247.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4721..4861
                     /locus_tag="WHQ94_000944"
     CDS             4721..4861
                     /locus_tag="WHQ94_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ2128248.1"
                     /translation="MDTLLKIIIALSFSGAEALVVWLFITAADAVLRRFQKPSHYRRV
                     KK"
     gene            4858..5067
                     /locus_tag="WHQ94_000945"
     CDS             4858..5067
                     /locus_tag="WHQ94_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ2128249.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(5125..6042)
                     /locus_tag="WHQ94_000946"
     CDS             complement(5125..6042)
                     /locus_tag="WHQ94_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ2128250.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6911..7267)
                     /locus_tag="WHQ94_000947"
     CDS             complement(6911..7267)
                     /locus_tag="WHQ94_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ2128251.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAM
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(7815..8195)
                     /locus_tag="WHQ94_000948"
     CDS             complement(7815..8195)
                     /locus_tag="WHQ94_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2128252.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8350..9546)
                     /locus_tag="WHQ94_000949"
     CDS             complement(8350..9546)
                     /locus_tag="WHQ94_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ2128253.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVTR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(9737..9826)
                     /locus_tag="WHQ94_000950"
     tRNA            complement(9737..9826)
                     /locus_tag="WHQ94_000950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9790..9792),aa:Ser,seq:cga)
     gene            complement(9930..10020)
                     /locus_tag="WHQ94_000951"
     tRNA            complement(9930..10020)
                     /locus_tag="WHQ94_000951"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9984..9986),aa:Ser,seq:tga)
     gene            complement(10103..10882)
                     /gene="yaaA"
                     /locus_tag="WHQ94_000952"
     CDS             complement(10103..10882)
                     /gene="yaaA"
                     /locus_tag="WHQ94_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ2128254.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(11144..12331)
                     /gene="dapC"
                     /locus_tag="WHQ94_000953"
     CDS             complement(11144..12331)
                     /gene="dapC"
                     /locus_tag="WHQ94_000953"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ2128255.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNPDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLALARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVF
                     LYR"
     gene            complement(12407..12859)
                     /gene="dut"
                     /locus_tag="WHQ94_000954"
     CDS             complement(12407..12859)
                     /gene="dut"
                     /locus_tag="WHQ94_000954"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ2128256.1"
                     /translation="MNIEVEMKVLDERMADFVPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            13002..>13414
                     /locus_tag="WHQ94_000955"
     CDS             13002..>13414
                     /locus_tag="WHQ94_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ2128257.1"
                     /translation="MTHTSSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNLAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKGIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
BASE COUNT         3663 a         3465 c         3066 g         3220 t
ORIGIN      
        1 ataaccaaag cattgaaagc ggagacttat ttttgtcgcc cttaccattc ttgggagaaa
       61 gggctgaatg agaacaccaa cggactcatc cggcaatact tccccaaaca aaccgatttc
      121 cgtaacatca gtgatcggga gatacgcagg gttcaagatg agttgaacca ccgaccaaga
      181 aaaacacttg gctacgaaac gccaagtgtt ttattcttga atctgttcca accactaata
      241 cactagtgtt gcacttgaaa tccgaatcca agcaatatta aaaattatcg tcatcaatgc
      301 ggcttcccca gcccgcggcg agctttccac gacaggcttt ttttatttgg gttggtcgtc
      361 tggtttcttt gagttcaaaa acaggtcggg atacttaagt tttattcgtg caggtatccc
      421 gcgcttcgtc caattgaaaa cgcattgagg gctattccct gttattcgac caacttccgc
      481 gtaactgccg attgattgca acaggcgttt gtcttcattg actcttttat ccataaataa
      541 accaaatgtt taaatctaat gctaatatta aacactatgt ttagataaaa atcaagtctt
      601 gtgtaacaac attttgttta aatatgggag aataattaag ccaaccgcga ataagattaa
      661 aaatgacaat gcacgagaca actgacagac tttttgagat agccaaagag cagggagttt
      721 taaagccggc tgacatagca gagcgtctga atatcagcca acaggctttg aaaaactggg
      781 aaagtcgtgg catagcggca aaggcgctgc ctgaagtagc aaaagcattc ggtgtatctg
      841 aaacatggct gagaacaggt gaaggcagcc gaaccgcgcc cgtccttatt gaccccgacc
      901 taccccacga agtcaaagac atccaccgcc cgatgacgtg gagcagcaac gacccgctgc
      961 ccgacgatga ttatgttttc gtcccctacc tcaaagagag ctgcttcaaa ggcggagtag
     1021 gcacgtatga aatccccgat tacaacggct accgcctgcc gttcggcaaa tccacgctta
     1081 aacgcaaagg catcaatccc gacaacgtgt tttgctgcac cctgaccggc gacagtatgg
     1141 aggaaaaaat cgcagaagac gcggcaattg ccgtagatac gggcgaaacc gccatacgcg
     1201 acggcaaaat ctacgccttc gcccaggacg gtatgttccg cgtgaagtac ctgatacggc
     1261 agcctggcaa cagcgttctg atacgcagcc acaacagcgg tttctatggc gacgaaaacg
     1321 cccccttgga cagcctgacc gttatcggca gggtattttg gtggagcgtg ctggattgaa
     1381 aaatgttgct attacgacga gataatattg atagggcttt caagattgtc aaaaataggc
     1441 gttttgattc cccttggtgg cctggtgagt acgatgccgg tatgaatttc cttggcgtac
     1501 aaggagagtt gaaggttcac gagctgcatc acagaacagc aaccctgtgc tttgagtggc
     1561 ttggcgaagt atctgcgccg cgcagaaaag agaactacaa agacctcaag ccaaatgtat
     1621 tgtatgactt tgatggttcc ggtaagcatt ttgccaaccc tgacgcaagg tatctattgc
     1681 ccgtcggttc aagcggctta atcttaaaac atatccaaat cgatgacgaa gacactctgt
     1741 tgcggctatg gtgcgcacga aatatcccaa tgccacaccg gctatctaaa atcccgatgc
     1801 ttcggcagta ttatctaagc aaggcatggc acgaaattta tgctatcaac caacacctaa
     1861 ggaaaacgaa gctgatagtt gatgtggcat acgaccccac agattaaaca acccgcccgc
     1921 attatgcggg cttttttcat gccccgccga acctgaaaac aacacaaaac cgacaaagcc
     1981 gccttaatag gcggtttttt tgcgcctgcg aaatctcaaa aataaattct tttaataatc
     2041 aaaatgttta taaaccttaa acaaaaatct taaacagatt gttgcatatt attaaacgct
     2101 atgtttaaat acacccatcg aaacaaacaa cagccacatg gagtaccgac atggaaacca
     2161 tcaccatccc taaagaaaat ttcgacgctt tgctaaatct cgcaagaacc gccgtcgccc
     2221 tgaacgaaaa ttttattcca aaaataaatt acggagcatc caccatacca gcagccggct
     2281 ttgccgcact taacgagttt ggaatagccc gctccaccgt ggaatctttg gaaattctca
     2341 aaattaaata acctcctcgg tattgtcatc ccgtgatacc ggcaacctaa ctaacctctc
     2401 tttaaaggaa aatcaaaaat ggaaacccaa gaaaagaaat ttgttcctgc ctccaagcaa
     2461 ataccgaccg taaatagcgg ctggttagac cgtgaattag gcgaagccat gagtgaagcg
     2521 gtgcgtgcct gccttgctca cggtaaacag tcagaaatta ctgtcaagct caaaatccaa
     2581 ccccaaaaca tccaaagcgg aacggtaaaa atcagccacg atgtagcaac caaactgccc
     2641 aaagaaaaac gcgaaggcgg catcgtcttt gctacacctg acggcaacat ccaagccgac
     2701 gatccggcac aaggaaaatt gaaactcaaa caagtttcca atacgtcaag cacgttgaaa
     2761 atggtcagat ccaactaact caaacacaaa aggaaatcta aaatggaaac ccaagaaaac
     2821 atgattaaaa ccgccttaca agcagctcaa aaaccttttt ttgagtttgc accgaacaat
     2881 actccgcttg tattcacacc agaccaagac ggtggctgga gatacaaatc acaccccgaa
     2941 ttgatgcaaa acccgtaccg caagtgcggc aaattcctca tgcacgatac cgccagtctt
     3001 attaagtttg tacaaaaaca caaacaggac ggcacacaaa tctacattga tgccgatttc
     3061 aaatcagggc gtattgatgt caccgccgtc atcaatggcc atacgcaaca agctccgggc
     3121 tggctcgatt tttatgccgt atacaccccg caacacacta catcggcatc caactggctg
     3181 aacaacaatg cgcaccgcat gaatcagatg gagttttccc atttcctgac caacaatgcc
     3241 cgcaacatcg tatccaaaaa tcccggtaac gaaaattccg tttacccgac cgccgccgaa
     3301 gtattggatt tcgcgctcaa tctcgagtac accgaaaaaa ccaccttcaa acaaggctat
     3361 cgtgaacagg acggcagaat caactttacc ttccaatccg aagactcagg gcaaacagag
     3421 aaaaacctca aaatgtttga acgtttcggc atcgagttca ccccgcatca aggcggtgca
     3481 tcctactttg ttgaagccct gttgaaattc cgcatcgaca aaaacagcgg cgcactcgtc
     3541 ctgtggtacg aattgcagca aatcgatgct gtaatcgaac aagcggcaaa agacatttcc
     3601 gaagccgtac aaaaagcctt ccctgaaatc gacatctatt tcggcgtaat cgcctaaaaa
     3661 aaagtcgtct gaaatcgtga accttcagac gacctccaca aaaacccaaa tccgaaagac
     3721 cccaaaatga ccatcacaga agaatactcc atcgacatcc gagtaacctc agaacagggg
     3781 aaaaacgact acggctaccc caccgaacgc tacggatgcg acatcatcaa cacagacggg
     3841 gagctacttg tcggcataga gccagagtac aaaaccccct ttgccgccgt cagaaaggcg
     3901 ctcatctgtc tgacaaaaga caaccccaaa aacgctgcct gatattaagc cgttacccca
     3961 ataatgagga atcaaaatga aatacttgat ccgcaccgcc ttactcgcag tcgcagccgt
     4021 cggcatctac gcctgccaac cgcaatccga agccgcagtg caagtcaagg ctgaaaacag
     4081 cctgaccgct atgcgcttag ccgtcgccga caaacaggca gagattgacg ggttgaacgc
     4141 ccaaatcgac gccgaaatca gacaacgcga agccgaagaa ttgaaagact accgatggat
     4201 acacggcgac gcggaagtgc cggagctgga aaaatgaact ttcaggaaga aaacgagaac
     4261 atcaagaatg acatagagat taatctcttg cattccgctg aagccgacag cgtggaggca
     4321 atgatggact tggcggttta cgggctggcg gcgatggttg ccaaacacaa caaaacgcgc
     4381 ggcgagcagc ttgtgttaca ggcgattgcc ggtcatatgc ggaagctttt ggccgcgctt
     4441 cccgaaaccg agtcattggt gcagaccggg aaggtttacc gccgacttga agatttgatt
     4501 tttaaaacgt atttgccgga gcagtaaaga tgaacacgaa tcaagatacg attccatttg
     4561 gcggcaacct tgtgatttgc tgttcgacgg gaaacggcgg ggacgggcgg ttttcttgtc
     4621 tgattaccga tcagataccg gatttaaatc ttgtcagaag cgggcaggcg ttcccgatgt
     4681 tcatttatga gtacgaaact agagaaaaaa ggaaagcctg atggacaccc tattaaaaat
     4741 catcatcgcg ctgtcatttt ccggcgctga ggcattggtg gtatggcttt tcatcacggc
     4801 cgccgatgcc gtattacgcc gcttccagaa accgagtcat tacaggagag taaaaaaatg
     4861 aacatcaacg aactgggcgc aaggatagac cgcccgacca tccgcgaact gattgcctac
     4921 gcaacctgcc gcaaccgccc catttcaaac tcgacactgc tgcgtatgga aaaagacggg
     4981 cggataccgt gccgtctgaa aaccccactc acatcccccg tatgggacac ccgcgaagtt
     5041 ttggaagccc tggggttaca gcaataaaaa aacgcccgat tagggcgttt tcacattcag
     5101 acggcttttg tattccctac tgcatcaaac cgacgacagg ttgcggattt cgggcagcat
     5161 cgggcggatt tttgccgcgt gttccgcgtc ggcgtgtgcg tttaaggctt cgagggcgtt
     5221 tgcggcggct ttgaggcggc tgcgtgtttc cgcccagacc gtccacatcg ttaccgcctg
     5281 tttgcagccg agctgcttca gcggcaggga aacgtctctg cccatttgga tggcccacgc
     5341 gccgtatctg acggcaacgg cgaggtcgta cagggcgttg ccgctgatgg gcagggcagg
     5401 ttgcggtgcg ggcaatggtt cgcggtcgag gacttcgccg tacagcacgc cgttttcaac
     5461 tgccacgcgg cccatcacgc ttaaaacttc ggggaactgt tcggcaggta cttctttgta
     5521 gctgcatccg aacttgcttt tgacggcaga ccaaagggta atggcgatac gcgcctgcgc
     5581 ttctttgggt gcggatttgg tcagggcgtt gtgcagtttt ttgacggctt cgatttgttc
     5641 gccggtcaag ccgtccggaa gggcgcgggt gggtttgggg gtaactgttt ttatgccgtc
     5701 tgaaattttg ccgttaagta aaatttcaat ctgctcatcg caccaaaccg caaatttcgg
     5761 attaagccaa cgggcaaagt gaatagcgag tttgggatgc agccatgtgc cttgctcact
     5821 gccaccacgc ttcacgataa ctatttgatt tgcttccgtt aggatttttc tcctaacgct
     5881 taaattctca gcaagtgcag agatatattg ttgagtttgt tcacttttta ggtagtcttt
     5941 aggtaacttg ccaaagtgag atgcaatggc ggttgcattt aaaaaaccgt cttgacggaa
     6001 agatacaggg gtgttaccaa aattgagaac agatacgttc atgatagttt cctagtttag
     6061 tttcgaaaga cccaaatggg tggtcgggag gttcgaaaac ctactagaaa aggccggcgt
     6121 tattcccctt gcgggtattg tattccgcac cctcccgacc ataggaaact agttcggata
     6181 tatcgaaaca aacaaccaaa caagaaaact tatggacgta aaaaattcac gctgacgggg
     6241 taaatgccgt tctagtagag gttttcgacg cctcgtgttt cgtaatatat ccaatctttc
     6301 tattttctgc aagcataaaa aagccgccat gtggcggata gggtcgcggt cttcaacagc
     6361 atgtaggatt taggaatgcc caaattgggc gcaggggtgt tgaaaacacg caaacaggcg
     6421 cgccgccagc cttacgggta ggcgcacccc cacataggag taaatcgatt tgatacgtat
     6481 agacgtaaaa aattccactc tatcgtgttg gatatacgct gtttgtcagg tgttttcaag
     6541 caccgtggga aatattatat agagttatgg aaatgtgtca agagaattag cccaaatggg
     6601 cggacgcgcg gttaagaacc cacataggag ggcggactta ttcccctttc gggtcttgta
     6661 ttcgtcgccc acgcggtcat agaaacttcc tgctatcgaa acaaacaaca aggaaagaaa
     6721 actatagaca tgaaaaaatc acattgacgg agtgattgcc gctgtagtgt ggtttctgac
     6781 gccacgaaca ggaatataaa acaaaacccc ctgcacatgc aaggggtttc ccaaaaaacc
     6841 gtaggcggca aactgaaagg ccgtctgaat ttcagacggc ctatgttgcg gcggatgtta
     6901 agctattaag ctatttggtt ttcagaggct ttttcttcca ttttcgggct acggcttcaa
     6961 attcttgaaa ggccgtcggt gcgctcctga tacggcgtat ttcaaaaata aagctgctta
     7021 ggttgtccca gtcacgtaac agcatggagt attcgaaatc tttatacatt ttctcgtgca
     7081 gtgctccgcc taatacgccc gcacagacaa attcgcgctg gtttaaaact gtcagcatgg
     7141 cttcacggtc tttcttcttg tccggggtat ccgatgtata ggtggcgagt atgcatccgt
     7201 ctgtttttgc cagcccgttt actatggtta tggcttcttg aagggcggca ttgttacgtt
     7261 cggccatgat cagcgcgctt tttggcgttt tctgtattcc tcatgatgcc gtaagcggca
     7321 acaaaaacgc cgataacagt caatatcggc gttgctatct gtattaggtt gtcagtcatt
     7381 gccgtcccag ccgtctgaaa ggcggaaatc acggctttcc gaataaatat gtcccattgt
     7441 catcccctta agctgatgtt gaaaataaat ccttgcgtcg gaatatacgg cattgcttga
     7501 ctattttcaa gattctgtac ctcgtgaacc ggactataca aaaaaaccgc cttcgtgtaa
     7561 agcggttctt aaatcgcccg ttaaggcgga cacaacgata gccaaactgg aacaggaggc
     7621 ggcaaaggcg ggtttgtcca aaacagcctt gattgaaagg ctgattgggg atttgtaggc
     7681 gtttgcagat acgacaaggg gcgcattccg cgccccatca cacgcacgga cggcttgtca
     7741 tcgcctgtcg cccgtgcgcg gctgcccata tttcagaaca gtcttgcaag cccctttaag
     7801 ggaacggttt tattctagta cagtttgaat gccttggcaa cggctgtttt aatcgcctga
     7861 aaatcctctt cactgattat tggaatgcag cggtcgcgcc ctttgggttt gtatcggtct
     7921 aatcgtgcca atccgactgt tgccgtcatg tcgcattttg cccaacattg gatgtgcggc
     7981 ttgtccggta aggggtttcc actcattttg tggtggtagt ccgccaaagg gacaggttct
     8041 gtgctgctta agggtacgac cgttaccagt ttgccgttgt gcctgtttcg cgctatgacg
     8101 acgacagggc gtttcttgac catttccggt tcttcataac cgcgaaagtc gcacatgata
     8161 accgaacgtt cccttggttg gaattttaaa ggcattagcc gctcctgacg atgacaggct
     8221 gctattatag cagttcttac acaaaaaacc gccttcgtgt aaagcggttg caaaaaagcc
     8281 ttccaataaa aatgcggaaa cggtttttta tttgcgtttc cgcataatac aaacacacac
     8341 acacaaaaat cattttccgt ttttggcaag cctcaaaaca tcattccccc accgttccag
     8401 cgcatcctta cgctcctccc aaagctccga gcggttgtaa gccgtttgcg tcctcgtccc
     8461 caacgaatgg gcaagcagct tttccgccac atcattatcc accttcagaa cctcgcgcaa
     8521 ataagtacgc gcaagcgagc gcaaaccgtg cgccgtcgta tccagccccg cccgctgcat
     8581 cgccacgcgc ggactttcct tattgatatg cccttgaaaa ttcacacttt caaacacaaa
     8641 ccgcccgttc acattcaacg cccgcgcctg atccaacacc cactgcatcg cccgcgacag
     8701 cggcacatca tgcccccgcc gctccttttt ccgttcggcg gggatacgcc aaacctcccc
     8761 gtccaactcc gaccactcca tcaacgccgc ctcctgaatc cgcgtcattg tcaacagcag
     8821 ccaatagata caaagccgcg ttacaggatg gatacgcacc ctgcccgcat aaaccgcaaa
     8881 ttcattgcgc tgctccaaaa aatcaatcag gcgcggcaat tcagacggac tcaacgctgc
     8941 catattcccc gttttcgccc gttcaaacac acccgaaccg atttgcgcga cagggttgat
     9001 ttccatcatt cccgaaccga ccgcaaacgc aaacaccatc ttcagactgt ttttcgtttt
     9061 gcgcaacgta tcaacgatac cgcgcgcctc catcacacgg aggcagccga ccacatccgc
     9121 cgtcctgacc tggcgaatat caagattgcc gatagccgga aaaacccacc gttcaaaatt
     9181 ccgcataacc tgtccggcat acttttcaga ccgccccttc gaccaacgca caaaccaatc
     9241 acgcgtcacc ttctcaaaag caaaatccgc ccgcaccttc ttattgacga cattttcccc
     9301 gtgcgcccgt tttcggcgca cctcctcccg ccattcccgc gcatcggcca gcgaaaaatc
     9361 aggataccgc cccagcgaaa ttgtctgctg ccttccatcc tgcacaaacg acagcttcca
     9421 actccgcccg cccgtcgggt agacccacaa agccagcccg cccccgtctg acaacttata
     9481 cagcttatcg cgcggcttcg catttttaac ctgatttgcc gacagcggcg taatgatttt
     9541 cgccattatg gtaatttccc acttatccaa aaattaccat atacattacc ataaaaaaat
     9601 gcaataagaa gataccgcaa gattgcaaat gatttacttt gacgcgtttt gatagggcaa
     9661 accgatgatt tttatatggt aattgacaca acttgaacaa aaaagaaccg ccctgaatca
     9721 gggcggtttt gttttctggc ggaaacggtg ggattcgaac ccacggagga tttgcaccct
     9781 cagcggattt cgagtccgct gcattcagcc tctctgccac gtttccgaaa gatgtttcaa
     9841 cctaaaagga aacacgatga agtaaaacca aataaaaatg ccgatgcttt atgctacttc
     9901 atttttattt ggtttttgac ctcaaggatt ggcggaagcg gtgagattcg aactcacgga
     9961 gggctatcaa ccctcgacgg ttttcaagac cgttgcatta aaccgctctg ccacgcttcc
    10021 gtcttcttga agagtaggaa taataatgaa gtttatacat tttgccaagc acttttttcg
    10081 gaaaatactt aatttattgt tttcatttta tttgttccga acgcataaaa acccattcgc
    10141 tttcatttga agccgcgtca ttgaatgcgt agccttcgta attaaaattt ttcagcattt
    10201 cagggtcggt aatatgatgt tctaccgcat agcgcaccat taatccgcgc gcgcgcttgg
    10261 catagaaact gatgatttta tatttaccgt ttttttcgtc tttaaatatc ggggtaatca
    10321 gccgcgcccg aagttttttc gtgttgacgg acttgaaata ttcctgtgag gcaaggttga
    10381 caagcgtatt gctgcctgct tgggcaagcg tgtcatttaa aaggttggta atgatgtcgc
    10441 cccaaaactc atacaaattc ttgccgcgca aattggcaaa tgccgtcccc atttccaaac
    10501 ggtagggctg tatcaggtct aacgggcgaa gaagaccgta cagaccggac agcaggcgga
    10561 catggttttg cagatagcgt atctgtccaa tatccaatgt gtttgcatcc ataccttcgt
    10621 aaacatcgcc gttgaacata aagaccgcct gtttggcgtt ttccggcgta aacggcgtgt
    10681 gccattctgc attgcgctgc gcgtttaaga gggcaatttt gtcggaaacg tgcatcagtt
    10741 cggcaatctg ttgcggcgca agctcgcgca actgctgcat tagaatgtcg gactctgcca
    10801 gcaggtcggg ttgggtaaac tcgctgacag gggcagggtc tttttcatta aggttcttcg
    10861 caggggaaag gacaaaaaac ataatctgct cggtattgtg tcaaacacgg ggattttaag
    10921 tttaaacgtg tttgcgggca agccgattaa aagtgaagta tagtggatta aatttaaacc
    10981 agtacagcgt tgcctcgcct tgccgtacta tctgtactgt cttcggcttc gtcgccttgt
    11041 cctgattttt gttaatccac tataaatacc gttatagcaa attcaaccgt aaaatattga
    11101 aaaatgccgt ctgaacaaaa gttcagacgg catttccgat tccttaccga tataggaaaa
    11161 caatggtttc cgcagctttg acacaagttg cgacatcggc aaccaaagcg atacgcacat
    11221 aaccttcccc gggattgccc tgttcggtat cccgcgccaa aaaacgtccg ggcaatactt
    11281 ggatagccgc tttttgccat aaattgcgtg caagtgccaa atcgtcgcca tcagggactt
    11341 tcaaccagat gtaaaacgag gcatccggta atttaacgtc aaatacctgt tgcaaaatgg
    11401 gaataacgcg ctcaaatttt tcctgataca tacggcggtt gtcgataacg tgctgttcat
    11461 catcccaagc agcaatgctt gcgcgctgca cgggaatact cattgcactg ccgtgatagg
    11521 ttctgtaaag cagaaagttt ttaagcagtt cggcatcgcc ggcgacaaaa ccggaacgca
    11581 ggcccggaac gttggaacgc ttggacaaac tggtgaacat aagcagtttt tgcctgcttc
    11641 gacccaactg tgcagcggct tgcaggcagc ccaaaggttt gttgccgtcg aaatagattt
    11701 cggaatagca ttcatccgag gcaataatga aaccatattt atcctgtaaa tcaaaaactt
    11761 ctttccagcc gtccaaatcc agcacgctgc cgctggggtt gttgggtgag cagacgaaca
    11821 ccagtttggt gcgtttccaa acctcttcgg aaatactgcg ccaatcgggg ttgaaagacg
    11881 gcgcggggca attagcaaaa tggatttcac cgccgcccaa aagtgtcgca ccttcataaa
    11941 tctgataaaa gggattcggg ctgacgatta cgggtttgag gccgtctgaa acagggttca
    12001 acacggtttg gacaaaagaa aacaacgcct ccctacttcc caaaaccggc agaatttcgg
    12061 tgtcgggatt cagcgtcaag ccgtcataac ggcgttttaa ccagttcgca cacgcctgac
    12121 gcagttcagg caaaccggcc gtcagcggat atttttccaa ctcgtgcaat gaggcggtca
    12181 gcgcatccgt aatgactttc ggtgttggat gtttcggttc gccgatatgc agtgggacgg
    12241 cttccagacc ttcgggggcg gaaatgccct gcatcgcttc acgcagtcgg gcaaagggat
    12301 agggtttaag ctgcttgagt aaggtattca tgattttctc cggttgtcgg tatatcggaa
    12361 gatatgccgt ctgaacctga cgtgcccttc agacggcatt ttatattcaa tgcaaacccg
    12421 tactgccgaa gccgccctca ccccggctgc ttccgacaaa ttcctcgaca cgtttgaaac
    12481 gcgcctgcac gattggcacg acaaccatct gcgcgatacg ctcgaacggc ttgacggcaa
    12541 atggctcgct gcttctgttc cataacgaca ccttcaattc cccttgataa tcggagtcaa
    12601 tcaaaccgac caagttaccc aagacaatac cgtgtttatg ccccaatccg gaacggggca
    12661 gcaaaacggc ggcatatgca ggattggcca aataaattgc caaacctgtc ggcacaagaa
    12721 acgtttcacc cggctgcaaa acgacttcct catccaaaca ggcgcgcaag tccaaaccgg
    12781 cagaaccctc cgttgcatag gcagggacaa aatccgccat cctctcgtcc aacactttca
    12841 tttctacttc aatattcatc gtcttgctcc tgcatacggc cagtttcaaa gatgggagat
    12901 tatacacaag ccgtgtttac gctgaaacac ggcaaatagg ttttgttctg aaatacaccg
    12961 tctgtgtata atgctcgacc ttcttacata tcaatattaa catgacacat acaagttcac
    13021 cccgtaacga atttatacgc ggcatcaaag aaagttcgcc catgctgatt gggcttttgc
    13081 cttgggcatt aatactcggt atgcagggcg gacaaaaagg catgagctgg ctggaaatgt
    13141 tgttgatgac cagtatgaac ctcgccggcg gctccgagtt tgccacggtc aacctgtggg
    13201 cggaacctct gccgatactg cttatcgcca ccgtaacctt tatgattaat tctcggcata
    13261 tcctgatggg ggcggcgctt gccccgcacc tgaaaggaat accgctgaaa aaagccgtgc
    13321 ccgcactgtt ttttatgtgt gatgaaagct gggcgatggc attctccgaa atccaaaaac
    13381 ggaaagcagc cggtttgccc gcattcaata tgcc
//
LOCUS       DAWGJV010000046        13388 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWGJV010000046 DAWGJV010000000
VERSION     DAWGJV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.046"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..289)
                     /locus_tag="WHQ94_000956"
     CDS             complement(<1..289)
                     /locus_tag="WHQ94_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128258.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFY"
     gene            complement(348..580)
                     /locus_tag="WHQ94_000957"
                     /pseudo
     CDS             complement(348..580)
                     /locus_tag="WHQ94_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            516..743
                     /locus_tag="WHQ94_000958"
     CDS             516..743
                     /locus_tag="WHQ94_000958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128259.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            905..1960
                     /gene="aroG"
                     /locus_tag="WHQ94_000959"
     CDS             905..1960
                     /gene="aroG"
                     /locus_tag="WHQ94_000959"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ2128260.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            2028..2516
                     /locus_tag="WHQ94_000960"
     CDS             2028..2516
                     /locus_tag="WHQ94_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ2128261.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2866..3342)
                     /locus_tag="WHQ94_000961"
     CDS             complement(2866..3342)
                     /locus_tag="WHQ94_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ2128262.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(3354..3782)
                     /locus_tag="WHQ94_000962"
     CDS             complement(3354..3782)
                     /locus_tag="WHQ94_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ2128263.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            3894..4139
                     /locus_tag="WHQ94_000963"
     CDS             3894..4139
                     /locus_tag="WHQ94_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128264.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVR
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            5164..7281
                     /locus_tag="WHQ94_000964"
                     /pseudo
     CDS             5164..7281
                     /locus_tag="WHQ94_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            7523..8989
                     /locus_tag="WHQ94_000965"
     CDS             7523..8989
                     /locus_tag="WHQ94_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2128265.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEAKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(9190..10158)
                     /locus_tag="WHQ94_000966"
     CDS             complement(9190..10158)
                     /locus_tag="WHQ94_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128266.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            10236..11528
                     /locus_tag="WHQ94_000967"
     CDS             10236..11528
                     /locus_tag="WHQ94_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ2128267.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(12031..12717)
                     /locus_tag="WHQ94_000968"
     CDS             complement(12031..12717)
                     /locus_tag="WHQ94_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ2128268.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(12793..13266)
                     /gene="queF"
                     /locus_tag="WHQ94_000969"
     CDS             complement(12793..13266)
                     /gene="queF"
                     /locus_tag="WHQ94_000969"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ2128269.1"
                     /translation="MSRNNEELQGISLLGNQKTRYPTEYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDSQ"
     regulatory      complement(13270..13314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3314 a         3255 c         3767 g         3052 t
ORIGIN      
        1 tatagaacgt tgcggctacg cggaagccgt actcgttgga tttggagcgg cccattttgg
       61 ttttgtcacc gtccaagaca atctcacggg gtttgtagat tgttttgtga cggtagtatg
      121 gatcaaactc gagaccgacg ctgtcggtca actgtttgcc tacattcaga ccgataccga
      181 cactccaacc tttggcgctt ttgctgacat cgcgggaagc acccatctgg gtcgtcatca
      241 ctttggtttt gccgcgcaaa tctgcatatg catccgccca aggggtcaag gatcatccgt
      301 cccccaaatc ttggcggatt tcgccatgga ctttcaaagc aaggttttca tgcttggtaa
      361 cggtgttttt ccttatcgcc gatgatggct ttgcccttgc cgttagactc gggaatatcg
      421 gctaccgtaa cggcggacac ggctgcaagt gagagtgcaa gcagggtttt ttcatgtttt
      481 tcttcctata atgaggatga ataaatggag aaagtgtggg aaatacccgc attccaatta
      541 aatctttttt caagcaatta gttctttttg ttttcaacat tttccttgcg tatgaaattt
      601 tgccggatgt gcaggcggaa tcggcagcgg ggtgtcctgt accggattgt cgtggaaacg
      661 ggaaaacgga tgttccgtgc ggttttgtcc gaatgaatgg cgggtattgt ttttatcaat
      721 ctgtttcttt ttctttggaa taaaattcct aaaatattaa aaatatgaaa tttaaaatct
      781 atgaaaaaag atatatcagt tattttgaaa taaaatagct ttgtagtaat atgttgcact
      841 tgtttgtgca aggtaaacga tgtaacctaa gccgcgtata aaaaaccatc aggaaagatg
      901 caagatgaca caccattacc ccacagacga tattaagatt aaagaagtta aagagttgtt
      961 gccgccgatt gcccatcttt acgagctgcc gatttccaaa gaggcttcgg gcttggttca
     1021 ccgcacccgt caggaaattt ccgatttggt tcacggcagg gacaagcggc tgttggttat
     1081 tatcgggccg tgttcgattc acgatccgaa agcggcgttg gaatatgcgg agcgtttgtt
     1141 gaaactccgc aagcagtatg aaaacgagct tttgattgtg atgcgcgttt atttcgagaa
     1201 gccgaggacg acggtgggtt ggaaaggttt gattaacgac ccgcatttgg acggtacgtt
     1261 cgatatcaat ttcggtttgc gtcaggcgcg cagcctgttg ttgtcgctga acaatatggg
     1321 tatgcctgcc tctaccgagt ttttggatat gattacgccg caatattatg cggacttgat
     1381 ttcttggggg gcaatcggtg cgcggacgac cgaaagccaa gttcaccgcg aattggcaag
     1441 cgggctgtcc tgccccgtcg gctttaaaaa cggtacggat ggcaatttga agattgccat
     1501 cgacgcaatc ggtgcggcga gccattcgca tcatttcttg tctgtaacca aggccggaca
     1561 ttccgccatt gtccataccg gcggcaatcc cgactgtcat gtcattttgc gcggcggaaa
     1621 agagccgaat tatgatgcgg gacacgtcag cgaggcggcg gaacaactgc gtgcggcagg
     1681 ggtaaccgat aagctgatga tagattgcag ccacgccaac agccgcaagg attacacccg
     1741 gcagatggaa gtggcacaag atattgctgc ccaattggaa caggacggga gcaatattat
     1801 gggcgtgatg gtggaaagcc atttggtcga aggcagacag gataagccgg aagtgtacgg
     1861 caaaagcatt accgatgcgt gtatcggttg gggcgcgact gaagaactgt tggcattgtt
     1921 ggcaggtgca aacaaaaaac gtatggcgcg cgccggttga gatttttgac acggaatgtc
     1981 ataaaatgcc gtctgaagcg ttcagacggc atttttgtgg aggaaatatg ctcaaaataa
     2041 ccctaattgc ggcgtgtgcg gaaaacctgt gcatcggggc gggcaatgct atgccttggc
     2101 acatccccga agatttcgca tttttcaaag cctatacctt gggcaaaccc gtcattatgg
     2161 ggcggaaaac gtgggaatcc ctgcccgtca aacccctgcc cggacggagg aacatcgtca
     2221 tcagccggca ggcggattat tgcgcggcag gcgcggaaac ggcggcaagt ttggaggcgg
     2281 cattggcatt gtgcgcaggc gcggaagaag ccgtcattat gggcggcgcg cagatatacg
     2341 gacaagcgat gccattggcg accgatttgc ggataaccga agtggatttg tctgtggaag
     2401 gagatgcatt tttccccgca atagaccgga cgcattggaa agaagcagag cggacggaac
     2461 gccgtgtcag cagcaaaggc acgcgctatg cttttgtgca ttatttgaga tattgaaata
     2521 taaactctct ataaaatccc ccgcaaatga tgggctgaaa tagaaaatat tgttattccc
     2581 ccgaagatgg gaatccggga ttttaaagtt agggtaattt atccgaaata acaacaatct
     2641 tccatcgtca ttcccgcaaa agcgggaatc cggaaacgaa aagctaaagc aatttatcgg
     2701 aaaaaaccga agtttaaaga accggattcc cgcctgcgcg ggaatgacga gattttaggt
     2761 tatggggatt tattgggaat aatggaacaa agaaagcaga aataaggata tagaggctgt
     2821 ctttggattt gcgatggttg tcggagaatg ccgtctgaag ccgtttcaga cggcattttt
     2881 ccagcttgag aacggatgcc tgctcaaata ggcattggta aacataccgt cggcagtgat
     2941 ttcccgtccc agccagtccg gacggtcaaa atcggcattc tcgtcgggca actcgatttc
     3001 cgcgacgacc aaaggcgcat tatcgccaag aaaaacatcg atttcaaaca ggctgccgcc
     3061 ccatctgacc ggataacgcc atttttccat tttaaacggg cacatcgttt ccatcatctt
     3121 ttccgcatcg gcaagcggga tttcgtattc aaactcactg cggctgattt ccgaaatata
     3181 gcctttcagc gtcagccacg cctgttttcc ggcaatgcgg acacggacgg tgcgttcttt
     3241 ttcaacagac agataaccct gcctcaacag cagcggttcg tcggcgtatt gccgccagtt
     3301 gtcgtttcca atcaaaaaac ggcgttcgat ttctatcggc ataagatgct ccgtcaaaac
     3361 ggtttgaaca cgaccagata cagcgcggca accatcagca gcacggggat ttcgttgaac
     3421 acgcggtacc agcggtgtga aaaagcattg ctgtaatcct gaaaacggcg cagcagcacg
     3481 ccgcaataca actggtaagc caagagcatc aagcccaaac acagtttgac gtgtacccag
     3541 ccgctgcccc accagccggc ggcaaacggt atcgccgcgc cgaacacgac cgcgccgaag
     3601 cccaacggcg acataaaacg gtacagccgc accgccatgc ccgacagacg cacatactcg
     3661 ggattgccgc gcggcacatc aatcatcgcc atattgacga aaatcctcgg caggtaaaac
     3721 agccctgcaa accacgaaat gacaaaaaac aagtgaaaca gcttgaacca agaaaacatc
     3781 atcgcccaca ccctgccgaa aagcggtatt gtacaggcaa accgcttggg aaacgtgata
     3841 aaatcaggcg gataaacaaa tcgaataaat ccttaccgca aaacggaggc aaaatgctca
     3901 aatccatcga actcaattcc cacatccgca accgccttgc agaatatctg aaaggcaggg
     3961 gtatggattt tcagacggca atgcaggaag aaaaaggcaa caaagaaatc gccgccatcg
     4021 tccgcagcgg tttgcccact ctggtccgca aactgtattc cgaacaaaaa atgcagaagt
     4081 ttttttggga aaagcgggat ttgattgccg actacatcag ccgccggatg cagggatagg
     4141 tggctgaaat ctgttttcag gcaagtgaaa agacaatatg gcagattgaa attacgctta
     4201 tcgtcattcc cgcccgcgcg ggaatccgac ttgtttggtt tcggttattt ttcgtttcgt
     4261 aacttttgag ccgtcattcc cgcgcaggcg gtaatccggc ttgttcggtt tcggttcttt
     4321 ttctcgtttc gggtgatttc taaaccgtca ttcccgcgca ggcgggaatc cggcttgttc
     4381 ggtttcggtt ctttttctcg tttcgggtga tttctaaacc gtcattcccg cgcaggcggg
     4441 aatctaggtc tttaaacttc ggttttttcc gataaatttt tgccgcatta aaattctaga
     4501 ttcccgcttt cgcgggaatg acggcggagg gtttttagtt ttcccgaaaa tgcacatcat
     4561 ccaaaatccc gttattccca caaaaacaga aaatcaaaaa caacaatctg aaattccgtc
     4621 cttccctccc gtgcgggaat ccgacttgtc cggtttcggt tctttttctc gtttcgggtg
     4681 atttctaaat cgtcattccc gcgcaggcgg gaatctaggt ctttaaactt cagttttttc
     4741 cgataaattc ttgccgcatt aaaattctag attcccgctt tcgcgggaat gacggcggag
     4801 ggtttttagt tttctcgata aatgcacatc atccaaaatc ccgttatttc cacaaaacag
     4861 aaaatcaaaa acagtaacct gaaatcccgt cattcccgcg caggcgggaa tccggcttgt
     4921 tcggtttcgg ttctttttct tgtttcgggt gatttctaaa ccgtcattcc cgcgcaggcg
     4981 ggaatccaga cctttaaacc ccgaccatcc ttgataaatt cttgcggcat taaaattcta
     5041 gattcccgct ttcgcgggaa tgacggcgga gggttttttg cttttcctga tttttcattg
     5101 cgatgtagta taatgtagta tataatcatt ataattttaa cacttgacaa aggaaaattt
     5161 ctcatgacac tgaaagcaag caagcaagca agcaagcaag caagcaagca agcaagcaag
     5221 caagcaagca agcaagcaag cggtcgggtt aatctattaa cattatctgt tttatcgctg
     5281 ttttgcacgc catatgtttg aggttcggat gcgtacgatc ccgtcaaaga agccgagatt
     5341 aaaaacaaat ttattttaga agcggcggaa gacagaaatt cccacgtttg gcgcggcccg
     5401 tgcagcatat cttttgattg cttcggtatg ttcagagctc agcttggttc aaatactcgt
     5461 tctaccaaaa tcggcgacga tgccgatttt tcattttcag acaagccgaa acccggcact
     5521 tcccattatt tttccagcgg taaaaccgat caaaattcat ccgaatatgg gtatgacgaa
     5581 atcaatatcc aaggtaaaaa ctacaatagc ggcatactcg ccgtcgataa tatgcccgtt
     5641 gtcaaaaaat atattacaga gaagtatggg gctgatttaa agcaggcggt taaggaacaa
     5701 ttaaaggatt tatacaaaac aagacccgaa gattgggaaa aaaataaaaa aatagttgaa
     5761 gaagcgtaca tacaaaagtt tggagaaaga tttaagaaac ttaaacagga acaacccaat
     5821 ataattaatc cgttgataga agacgccgta cttactcctt atacatcaca gactaatctc
     5881 gacaacatct tcaataaaac actgcacgtc aaaatcgaaa acaaatccca cgtcgccgga
     5941 caggtgttgg aactgaccaa gatgacgctg aaagattccc tttgggaacc gcgccgccat
     6001 tccgacatcc atacgctgga aacttccgat aatgcccgca tccgcctgaa cacgaaagat
     6061 gaaaaactga ccgtccataa agcgtatcag ggcggcgcgg atttcctgtt cggctacgac
     6121 gtgcgggagt cggacgaacc cgccctgacc tttgaagaca aagtcagcgg acaatccggc
     6181 gtggttttgg aacgccggcc ggaaaatctg aaaacgctcg acgggcgcaa actgattgcg
     6241 gcggaaaagg cagactctaa ttcgtttgcg tttaaacaaa attaccggca gggactgtac
     6301 gaattattgc tcaagcaatg cgaaggcgga ttttgcttgg gcgtgcagcg tttggctatc
     6361 cccgaggcgg aagcggtttt atatgcccaa caggcttatg cggcaaatac tttgtttggg
     6421 ctgcgtgccg ccgacagggg cgacgacgtg tatgccgccg atccgtcccg tcaaaaattg
     6481 tggctgcgct tcatcggcgg tcggtcgcat caaaatatac ggggcggcgc ggctgcggac
     6541 gggcggcgca aaggcgtgca aatcggcggc gaggtgtttg tacggcaaaa tgaaggcagc
     6601 cgactggcaa tcggcgtgat gggcggcagg gccggccagc acgcatcagt caacggcaaa
     6661 ggcggtgcgg caggcagtta tttgcatggt tatggcgggg gtgtttatgc tgcgtggcat
     6721 cagttgcgcg ataaacaaac gggtgcgtat ttggacggct ggttgcaata ccaacgtttc
     6781 aaacatcgca tcaatgatga aaaccgtgcg gaacgctaca aaaccaaagg ttggacggct
     6841 tctgtcgaag gcggctacaa cgcgcttgtg gcggaaggcg ttgtcggaaa aggcaataat
     6901 gtgcggtttt acctgcaacc gcaggctcag tttacctact tgggcgtaaa cggcggcttt
     6961 accgacagcg aggggacggc ggtcggactg ctcggcagcg gtcagtggca aagccgcgcc
     7021 ggcattcggg caaaaacccg ttttgctttg cgtaatggtg tcaatcttca gccttttgcc
     7081 gcttttaatg ttttgcacag gtcaaaatct ttcggcgtgg aaatggacgg cgaaaaacag
     7141 acgctggcag gcaggacggc gctcgaaggg cggttcggca ttgaagccgg ttggaaaggc
     7201 catatgtccg cacgcatcgg atacggcaaa aggacggacg gcgacaaaga agccgcattg
     7261 tcgctcaaat ggctgttttg atgcgtcggg aaatgttttg acgcacaggc ggcacaccgg
     7321 cacggccccg cgcgccgccc cgcaaaccaa tccgaaccct gccgccccga agggcggggc
     7381 ataatgatga aaccggcgga aaaccgccgg ttttttgctg ccgtctgaaa cctgattctg
     7441 gcttcagacg gcattgtcgc ggcatcgggc ggcagggttt ggaacagcgg cataaaaaac
     7501 tgatacaatc cgccggttga taatggttat tttttatttt tgtgggaaga catttatgcc
     7561 tgcacgaaac agatggatgc tgctgctgcc tttattggca agcgcggcat atgccgaaga
     7621 aacaccgcgc gaaccggatt tgagaagccg tcccgagttc aggcttcatg aagcggaggc
     7681 caaaccgatc gacagggaga aggtaccggg gcaggtgcgg gaaaaaggaa aagttttgca
     7741 gattgacgga gaaaccctgc tgaaaaatcc cgaattgttg tcccgcgcga tgtattccgc
     7801 agtggtctca aacaatattg ccggtatccg cgttattttg ccgatttacc tacaacaggc
     7861 gcagcaggat aagatgttgg cactttatgc acaagggatt ttggcgcagg cagacggtag
     7921 ggtgaaggag gcgatttccc attaccggga attgattgcc gcccaacccg acgcgcccgc
     7981 cgtccgtatg cgtttggcgg cagcattgtt tgaagacagg cagaacgagg cggcggcaga
     8041 ccagttcgac cgcctgaagg cggaaaacct gccgccgcag ctgatggagc aggtcgagct
     8101 gtaccgcaag gcattgcgcg aacgcgatgc gtggaaggta aacggcggct tcagcgttac
     8161 ccgcgaacac aatatcaacc aagccccgaa acggcagcag tacggcaaat ggactttccc
     8221 gaaacaggtg gacggcacgg cggtcaatta ccggctcggc gcggagaaaa aatggtcgct
     8281 gaaaaacggc tggtacacga cggcgggcgg cgacgtgtcc ggcagggttt atccggggaa
     8341 taagaaattc aacgatatga cggcaggcgt ttccggcggc atcggttttg ccgaccggcg
     8401 caaagatgcc gggctggcag tgttccacga acgccgcacc tacggcaacg acgcttattc
     8461 ttacaccaac ggcgcacgcc tttatttcaa ccgttggcaa accccgaaat ggcaaacgtt
     8521 gtcttcggcg gagtgggggc gtttgaagaa tacgcgccgg gcgcgttccg acaataccca
     8581 tttgcaaatt tccaattcgc tggtgtttta ccggaatgcg cgccaatatt ggatgggcgg
     8641 tttggatttt taccgcgagc gcaaccccgc cgaccggggc gacaatttca accgttacgg
     8701 cctgcgcttt gcctgggggc aggaatgggg cggcagcggc ctgtcttcgc tgttgcgcct
     8761 cggcgcggcg aaacggcatt atgaaaaacc cggctttttc agcggtttta aaggggaaag
     8821 gcgcagggat aaagaattga acacatcctt gagcctttgg caccgggcat tgcatttcaa
     8881 aggcatcacg ccgcgcctga cgctgtcgca ccgcgaaacg tggagtaacg atgtgttcaa
     8941 cgaatatgag aaaaatcggg cgtttgtcga gtttaataaa acgttctgat tgctgttcct
     9001 tttcggagga aaccctgccg gcggcggtat cacggcgggc atcggcggct ttcgggcggt
     9061 gctttgcgtg ccgccgcgtg tgcggaaacg cattccggtt tttccggcat aacggcgatg
     9121 cgaggtaaaa tgccgtctga aacccgattc gggcttcaga cggcattgtc gcggttgcgg
     9181 caggcgggtt caccagattc cgtcaaaggt tttcgcgccg cgccaaaatt tccacctgtc
     9241 ggcgggtttg aaggtcagcg taccgccgtg ttgtccgtcc gtggtgatgt ccagccgttt
     9301 gattttgccg gtacggacgg cttcgtaaag cggtgcgaac cagcgttctt cccactgctg
     9361 caatattgcc gcataccgct ccctgtcccc tgtcagggcg gtcaggcgca aatcgtccat
     9421 aaacaggata tggtgcgtgt caggcaggtg tgccgccgtt tcttcatagg cgcggaagtt
     9481 gtcgggcaat gcgcggcggt cggggtggaa acggctccaa accgtatcgg cgaaaagcgt
     9541 gccgccttgc gcgccgccgt ttgtgccgtc ccaaagccat aagccgttca actcgggcag
     9601 cccgcgtttt ttgcggttat ggttgacggg gtgcgccgcc agccacattt ggatttcggt
     9661 ttggacgcgc agccattcca acgcgtcttc cccgtccggt tgatcgtccg cacccaacag
     9721 tccgcccaag tccaaaacgg gcttcgcgcc ccagcggtac gcgcggggaa gggaaaccag
     9781 ccataattcg ggcaggacgg gaacgaaacg ccacggaata tcgccgtaaa acgccgacag
     9841 atcgcggcag agccgttccg cttcatccgt accgacgttc agatattccg ccgttagcac
     9901 atttgcctga tgcagcccca ttttttgcca gacgggcgtg gcgagcgcga cggtttcaga
     9961 cggcatattc aggctttgcg ccgcgcgttc cgccagtctg ccgcaccaca aataacgcgc
    10021 gtaaaatgcc gaagccgtgc agctttggcg gtgcagcgag ccgtattgca ggattttgtt
    10081 gaaggcgggc aggcagagag gtattcgggt ttcgtcttca tccaaattga gcgagggaat
    10141 ggcgagggtg agtttcatcg tttgacgttt cagaaatgca ggtcaggcgc aacattatag
    10201 aggattcggc gcaaacgccg tcaaaaagga acaatatggc tgtcttccca ctttcggcaa
    10261 aacatcggaa atacgcgctg cgcgcgcttg ccgtttcgat tattttggtg tcggcggcat
    10321 acattgcttc gacagagagg acggagcgcg tcagaccgca gcgcgtggaa caaaaactgc
    10381 cgccgctgtc ttggggcggc agcggtgttc agacggcata ttgggtgcag gaggcggtgc
    10441 agccgggcga ctcgctggcg gacgtgctgg cgcgttcggg tatggcgcgg gacgagattg
    10501 cccgaatcac ggaaaaatat ggcggcgaag ccgatttgcg gcatttgcgt gccgaccagt
    10561 cggttcatgt tttggtcggc ggcgacggcg gcgcgcgcga agtgcagttt tttaccgacg
    10621 aagacggcga gcgcaatctg gtcgctttgg aaaagaaagg cggcatatgg cggcggtcgg
    10681 cttctgaggc ggatatgaag gttttgccga cgctgcgttc ggtcgtggtc aaaacgtcgg
    10741 cgcgcggttc gctggcgcgg gcggaagtgc ccgtcgaaat ccgcgaatcc ttaagcggga
    10801 ttttcgccgg ccgcttcagc cttgacggtt tgaaggaagg cgatgccgtg cgcctgattt
    10861 acgacagcct gtatttccac gggcagcagg tggcggcggg cgatattttg gcggctgaag
    10921 tcgttaaggg cggcacaagg catcaggcgt tctattaccg ttcggacaag gaaggcggag
    10981 ggggcggcaa ttattatgat gaagacggca aggtgttgca ggaaaaaggc ggcttcaaca
    11041 tcgagccgct ggtctatacg cgcatttctt cgccgttcgg ctaccgtatg caccccatcc
    11101 tgcacacatg gcggctgcac acgggcatcg attatgccgc accgcaggga acgccggtca
    11161 gggcttccgc cgacggcgtg attaccttta aaggccggaa gggcggatac ggcaacgcgg
    11221 tgatgatacg ccacgccaac ggtgtggaaa cgctgtacgc gcacttgagc gcgttttcgc
    11281 aggcacaagg caatgtgcgc ggcggcgagg tcatcggttt tgtcggttcg accgggcgtt
    11341 cgaccgggcc gcacctgcat tacgaggcac gcatcaacgg gcagcccgtc aatcctgttt
    11401 cggtcgcatt gccgacaccc gaattgacgc aggcggacaa ggcggcgttt gccgcgcaga
    11461 aacagaaggc ggacgcgctg cttgcgcgct tgcgcggcat accggttacc gtgtcgcaat
    11521 cggattgaag tttgaaccgg cgacgaaaac aatgccgtct gaaaatctgc aaacaggttt
    11581 tcagacggca tttatagtgg attaacaaaa accagtacgt cgttgcctcg ccttagctca
    11641 aagagaacga ttctctaaga tgctgaagca ccaagtgaat cggttccgta ctatttgtac
    11701 tgtctgcggc ttcgttgcct tgtcctgatt tttgttaatc caccatacag tcgattaaaa
    11761 taaaactaag ccaaggaagc actgccgtca ttcccgtacc gtcgggaatc cggacaccac
    11821 ggtacggaaa cccatccgcc gtcattcccg cgaaagcggg aatctagaaa tacaatgcgg
    11881 caggagttta tcggaaatga ctgaaaccca acgcaccgga ttcccgcttt cgcgggaatg
    11941 acgaagtggg cgggaatccg gatttatccg ttccgacagt gtttgcaaat aaaagaaaac
    12001 ccaaccgtcc cgatttccgg cagggctgtt ttacggattt tgcagcgagg gcgcggggcg
    12061 gtcttgcgcc tgtttggttt gcagggtcgt cagttttttc gtcagcagat tcagaatcac
    12121 gccgtaggcg ggcaggaaaa acagaccgca gacggtgagt ttgaacaggt aatcgaccaa
    12181 ggcgatgccc tgccagtttg ccgccataaa tccatcgctg ctggcgtaaa aggcaacggc
    12241 gaaaaatacc agcgtatcca aagcgttgcc gatgacggtt gatgcggtcg gggcaatcca
    12301 ccacgctttc agacggcgta atttgttgaa cacaaaaata tcaaggattt gtccgagcgc
    12361 gtaggcggca aagctggcca gcgcgatgcg tccgacaaag gtgttgaatt cggacagtgc
    12421 gcccaagccc gtccaactgc cgttgtggaa caaaacggaa aagacatagg aaagcaaaag
    12481 ggcggggaac atgacccaaa agataatccg ccgtgccaag tgcgaaccga aaatgcggac
    12541 ggtcaggtcg gtggcaagga agatgaaggg aaaggaaaac gcgccccaag tggtgtggat
    12601 gccgaaaatt tggaagggga actgcaccag atagttgctg gcggcgatga tgaggatatg
    12661 aaaaagcacc agccggaaga gtgccttctg ttgctgtgcg tcggtaaatg cgtacataaa
    12721 aaaacctttc ggaaaggcgt tcagacggca taccgtatcg aaagaatgcc gtctgaaagt
    12781 gggaaggacg gattattggc tgtcgtgttc gaacaagcgt ttacgcgcca atgcttcaaa
    12841 ctcggtgcct gctttgccgt agttggcaaa cggatgaatg gcgatgccgc cgcgcggtgt
    12901 gaactcgccg aatacttcga tgtatttcgg atccatcagg gcaatgaggt ctttcatgat
    12961 gatgttgaca cagtcttcat gaaaatcgcc gtggttgcgg aagctgaaga ggtagagttt
    13021 cagggatttg ctttccacca ttttgatgtg cggaatatag cggatgtaga tggtggcgaa
    13081 gtcgggctgc ccggtcatgg ggcagaggct ggtgaactct gggcagacga atttgacgaa
    13141 atagtcgttg tcgggatgtt tgttgtcgaa tgcttccaaa atttcgggcg cgtattcggt
    13201 cgggtatcgg gttttctgat tacccaaaag cgagatgcct tgcagctctt cgttgttgcg
    13261 ggacataggg tttccttagt tttttagtgt gggaggtttt cgaaccacgg ggcggcgatt
    13321 gtaatataag cggcggtatc tgtgtagttt tcttcagacg gcatggtttg gacggcggcg
    13381 ttttccgt
//
LOCUS       DAWGJV010000047        13083 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWGJV010000047 DAWGJV010000000
VERSION     DAWGJV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.047"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..82)
                     /locus_tag="WHQ94_000970"
     CDS             complement(<1..82)
                     /locus_tag="WHQ94_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ2128270.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHII"
     gene            complement(83..1126)
                     /gene="galE"
                     /locus_tag="WHQ94_000971"
                     /pseudo
     CDS             complement(83..1126)
                     /gene="galE"
                     /locus_tag="WHQ94_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WHQ94_000972"
                     /pseudo
     CDS             complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WHQ94_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2152..4425)
                     /locus_tag="WHQ94_000973"
     CDS             complement(2152..4425)
                     /locus_tag="WHQ94_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ2128271.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRNENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEIGDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4489..5139)
                     /locus_tag="WHQ94_000974"
     CDS             complement(4489..5139)
                     /locus_tag="WHQ94_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128272.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5136..5933)
                     /locus_tag="WHQ94_000975"
     CDS             complement(5136..5933)
                     /locus_tag="WHQ94_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2128273.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5933..7096)
                     /gene="ctrB"
                     /locus_tag="WHQ94_000976"
     CDS             complement(5933..7096)
                     /gene="ctrB"
                     /locus_tag="WHQ94_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ2128274.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDVFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7111..8286)
                     /gene="ctrA"
                     /locus_tag="WHQ94_000977"
     CDS             complement(7111..8286)
                     /gene="ctrA"
                     /locus_tag="WHQ94_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ2128275.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8421..9555
                     /gene="neuC"
                     /locus_tag="WHQ94_000978"
                     /pseudo
     CDS             8421..9555
                     /gene="neuC"
                     /locus_tag="WHQ94_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
     gene            9559..10245
                     /gene="neuA"
                     /locus_tag="WHQ94_000979"
     CDS             9559..10245
                     /gene="neuA"
                     /locus_tag="WHQ94_000979"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ2128276.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10246..11295
                     /gene="siaC"
                     /locus_tag="WHQ94_000980"
     CDS             10246..11295
                     /gene="siaC"
                     /locus_tag="WHQ94_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ2128277.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11379..12866
                     /locus_tag="WHQ94_000981"
     CDS             11379..12866
                     /locus_tag="WHQ94_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ2128278.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
BASE COUNT         3781 a         3093 c         2671 g         3538 t
ORIGIN      
        1 gaatgatatg gcggacaacg gcagagccga taaagcccgc gccgccggta acgaggatgg
       61 tttttttgtt tgcggtttgc atttaatccc catatccgtt ggggttgcgg ctgacccaac
      121 gccacgaatc ctccatcatt tgcgcaaggc tgcgtttggt ttcccaacct gtctcttgtt
      181 tggtatggga cgggtcggca aaggaacacg ccaagtctcc ggcgcgtcgg ggttggattt
      241 gatagggaat atttaagccg gatgcggctt caaaggcacg gatgatttcc aaaacggaat
      301 aggcgcgccc cgaacccaag ttgaacaaat gtacgccggc aacgccgcct tttgcctgca
      361 tagccgcgac atggccttct gccaaatcca tcacatgaat atagtcacgc atccccgtgc
      421 cgtcgggggt agggtagtca tcgccaaata ccgccaattg cggcagtttg cctgccgcca
      481 cttggcagat ataaggcaac aaattattcg ggatgccgtt tggctgctcg ccaatcaaac
      541 cactttcgtg cgccccaatc gggttgaaat agcgcaacaa aatcacgctc caacgcggat
      601 cggccttttg gatgtcggtt aacatccgtt ccaccatcgc tttggatgcg ccataaggat
      661 tagcggtatc gcccgggcgc atatcttccg tatagggcat tttacgtatc atggcgcgac
      721 gcattaaagt aaacgccaaa gcgcgtttat actggttatc cagtttccct atatttttac
      781 gttacaaatc aagttgttgg tattcatcgt gaaaatagaa tttttcggca taacgctgac
      841 tcataaaacc ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg
      901 tttcatggtt gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt
      961 tagtaatcac acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa
     1021 atacatctgc aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt
     1081 tctctttatt accgatatag ttaattttcg gatattgctt attcattttc actcattttt
     1141 aaaatacttt cagcaatagc ttttgccaat aaaggcggta cggcgttacc aacctgctgc
     1201 tgctgggcaa tttttctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct
     1261 aattcacgaa cggttaacgc ccgattctgt tcatagtgaa aaactttgcg catatctcct
     1321 gtaatacaaa cggctggttt tgttgctgtt gtaacggatg tatttacgga tatcacctgt
     1381 tttcggacgt aatggttcag gaatatcgtt acggttacct ccatttttaa caaatgccat
     1441 tttttctaac atttgtgccg aatgattcat agcttcatga tttgcaacgt gtggattgct
     1501 ttcgccagca gccagttttg gaaaatgtcc tattgctgat ccaacagtct gatgggaaat
     1561 ctgcaaaggt tcgggaaagg aaattttgcc tttatccctc ctcccgataa atatcactcg
     1621 gctacgtatc tgaggaacac cgaaatcggc tgcactcagt atcttacatt ccaccgaata
     1681 accgatattc tgaaatgctt gaataatctc aatacgtgtt ttacctgaat tgtgtgtata
     1741 gagtcgcgct acattttcca taacaaaaaa atatggttgg acaattttaa ctattcggac
     1801 aaactcttta aataaatggt tgcgtgggtc atctgtaaat gtccgtccaa tctttcctgc
     1861 catactaaaa ccttgacaag gtggtcctcc aataatcaaa tcaactgctt gtccgttaag
     1921 acaattgatt aaatcttgtt cggttagtgt ggttaaatct ttttgcagta attgatgatg
     1981 ggggaagttg gtacggtaag tctgacaata atcagactcc atttcaacag aaagcaattg
     2041 ttggaatccg gcttgttcaa aacccaagga taggcctcct gctcctgaga aaaggtcaat
     2101 ataggtaagt ggtgtgtgca taagtcaaaa tccataaact cttcgattat tttaccgctt
     2161 cagcttcgca aacgcatccg ccatcgccga attggctggg gcgcggtcgt tgcgttgggg
     2221 tttgcggccg gctgtccgtt cgcggctgcg gttttcagac ggcattttat gttttgcgcc
     2281 gcccggttcg tcatccaagc gcatggtcag cgcgatgcgt ttgcgtgcag catcgacttc
     2341 cagcactttc actttcacca cgtcgccagc tttcaccact tcgcgcgggt cttggacgaa
     2401 cttgttggac agggcggaga tgtgcaccaa gccgtcctga tggacgccga tgtccacgaa
     2461 cgcgccgaag ttggcgacgt tggaaaccac gccttcgagt atcataccga cttgcaagtc
     2521 gccgatttcg tggatgcctt cggcaaacga tgccgtctga aacgcgccgc gcggatcacg
     2581 gccgggtttt tccagttcgg acaggatgtc caaaatggtc ggcaggccga agcgttcgtc
     2641 ggtgaagtcg gacgctttga tttgcttcac gcgctcgcgg ttgccgatga gttcggcggc
     2701 gctaatgcct tgttgcgcca gcattttggc gacgacggga taggcttcgg ggtggacggc
     2761 gctcgcgtcc aacggctctg tgccgccgtt aatccgcaaa aagcctgccg cctgctcgaa
     2821 ggttttttcg cccaaacgcg gtactttcag caattttttg cggctgtcga acgcgccgtt
     2881 ttcattgcgg taggcgacga tgttttgggc aagggtctga ttcaaaccgg aaatccgcgc
     2941 caagagcggg gcggaggcag tattcacgtc cacgccgacg gcgttcacgc agtcttcgac
     3001 caccgcgtcc agcgatttgg cgagctggct ttggttcacg tcgtgctgat actggcccac
     3061 gccgatggat ttggggtcga ttttgaccag ctcggcaagc gggtcttgca gcctgcgggc
     3121 gatggacacc gcgccgcgca gggaaacgtc caagtcgggg aactcgcgcg ctgccagttc
     3181 ggacgcggaa taaatcgacg cgccggcttc ggaaacgacg attttgtgca gtcccatttc
     3241 cggcattcca cgcaccagtt cgcccgcgat tttgtcagtt tcgcggctgg cggtgccgtt
     3301 accgatggcg atgagcttca cgccgtgctg tttaatcagg cgcgacagcg ttgccaacat
     3361 attgttttct tgatgcaaat agacaatgac ggtatccagc agcttgccgg tgtcgtccac
     3421 cacggcgcat ttcacgccgt tgcggtagcc ggggtcgaga cccagcgtgg tcagccgtcc
     3481 ggcgggcgca gcaagcagca agtctttgag attgcgggcg aacacggtaa tcgcgtcggt
     3541 gtcggcggct tctttcaaac gattgagagc ttcaagttcc aacgacaaaa agattttcgc
     3601 gcgccaagtc aggcgcacgg tgtcgcgcag ccatttgtgg ccgtctgaaa ctttgaagcg
     3661 gcgggcgatg atttgctcgt attcgctttg ctgcgtaatc ggcgtgtcgt cgggctgata
     3721 tttgagcgcg atgttcaaca cgccttcgtt gcgtccgcgc aaaaccgcta gcgcacgatg
     3781 gctgggcatc gcacgaatag gttcgcggtg gtcaaaataa tcgctgaatt tttcgccttc
     3841 ggtttctttg cctccaacaa cttgcgcgtg gatttcggct tcgttccaca gcttgtcgcg
     3901 cagcgtgccg atgagttccg cgtcttcggc aaactgctcc atcagaatcg cacgcgcgcc
     3961 gtccaacgcg gctttggcat cggggacgtt ttcgttcagg tagccttgtg cggcggcttc
     4021 cacgtcctgc gactgctcgg caagcaacac gtccgccagc ggctgcaaac cgtgttcgcg
     4081 cgcaatttgc gccttggtgc ggcgtttggg cttgtagggc agatacaggt cttccagcgc
     4141 ggttttgttg tcggcggctt cgatttgcgc gcggaggccg tctgaaagtt tgccttgctc
     4201 ttcaatgctt tttaacacaa cggctttgcg ctcttccaac tcgcgcaggt attgcagccg
     4261 ctcggcaagc cggcgcaact gcgtatcgtc caacccgccc gtcgcttcct tgcggtagcg
     4321 ggcgataaac ggcacggtcg cgccgtcgtc caaaagctcg acggcggcgg tgatttgcgc
     4381 ggcagtcgcg gagagttctt gggaaagaat ttgagtaatg ttcatcaata ggattccaac
     4441 ggacaggccg tctgaaattt cagacggcct gatttaaaaa caatcgcttt aaggcagcga
     4501 attataatat tcgtaggctt tgtccatatc ttcaaactgg tacatatgcc ctttttccag
     4561 caccatcgca ttatcgcaat attgcttcat ggcgctgtgg ctgtgcgaca ccaagatgat
     4621 ggaacggtct ttgcgctttt caaacaactc gtacttacat ttatcggcaa aacgcgagtc
     4681 accaactgca atcacttcgt caatcaaata gcaatcaaac tctaccgcca acgacaaagc
     4741 aaatgccaaa cgcgccttca tacctgaaga ataacgcttg accggctcgt aaagatattg
     4801 ccccaactcg gaaaactctt cggtaaagtt cttcacataa tcgatatcga cattgtaaat
     4861 ccggcagatg aaacgcaaat tgtccatacc ggtcagacta ccttgaaacg caccggagaa
     4921 tgccaaaggc caagaaatac tcattgtccg cttgatttca cccgtggtcg gcggctcaac
     4981 gccactgatc aaacggatga gcgtcgattt acctgcaccg ttgcggccga gaataccgat
     5041 tttctcgccc ttctccattt tgaagctaat atcgtgcaag actgtccgcc aaccttggcg
     5101 ggtcagatag cgtttggaaa cgtgttcaac tgaaatcatt gcggctcaac ccctttgctg
     5161 aatctcgcca ccaccgccaa accaagcagc agcaaaacca agttacacaa caaaatatac
     5221 caaggatttt cgtaagtcgt gacgctgtcg ccaaaatatc cggcacggaa catttctgtg
     5281 ccatgcacca tcgggatcat caatgcatac tcttgcactt tcggaggcaa attatgtaca
     5341 aaaaagaatg cacccgataa cggcatcatc acaaaagtca atgtgcccca aatcttgcca
     5401 aacggctcga aattaaaggc aatcgaacaa atcaccaaac ccaaaccaat cgcaaaaaaa
     5461 gccatcaaaa gccaagccat cagcatataa aacatatctg ccggcatttc aatccagcca
     5521 atcgcaatca ataccgccat aatcacaatc tgcgcaatgg ttgcaccagc aatttccaaa
     5581 atcatgcgcg ccaagatggt atccaaaact cttacattgc ggtgataaag caagctggca
     5641 tttgaagaaa tcgacccaac tgcccgtttt gaggcattac gccacatcat caacatcgga
     5701 tagccagtaa tcgcaaatgc gacaatattc aaagttgaat atcggtctgc ctttaaaaat
     5761 ttccacatca agacgataac gaatgtcatc agcaacggct caacaaacag ccataaaaag
     5821 ccaatattat tgcgaccgta acgggtgata atttcccgca tcaaaagcgc accgattacg
     5881 cgcctttgaa tggctaaaga ttcccaaaat gatgttttat gcaaggcttt catcagtttt
     5941 tatgctcacg aatgctggca gtcaacaggc tcaaaatacc ataaaccatc aagccgataa
     6001 tcagagtggc aacaatgttg tataaccgtt taggctcatg tgccaaatcc ggcaggctcg
     6061 gttgcgagat cacttccaaa taaagctgct gacggtctgc ttcaaccttg gcactttcca
     6121 aagaagtcat ggcggctgcc aactgctgct ctgccaactg gttttccaaa tacacacgct
     6181 gatattcggc agcctgatta gacaacgaag aatgcccacc gcccgaaatg gcacgtaact
     6241 gttggtcaat ttctttacgc aagctctgct cacgcgcctg caaacccgga atctgcggat
     6301 tctccggagt gactgctttc acctgatcca gctgggtttg aatcacaatc aattcatctt
     6361 gcagcttgga aaccaacccc atttgcactt ccgattgcgc tttcaaatca aaaacgccat
     6421 tggcaatccg gtaatccgtc agattctgag aggcttcctt tacccgctct gccgccgttt
     6481 tcactacttc ttccgcatag cgcaccgtat cagcacgtgc acgatcgttc aactggttaa
     6541 tcaatgcttc accttgtttt aacaaagcct cattgatttt cttagattcc agcgcatcaa
     6601 aggaagttac attcaacgtg gaaatacccg aaaccgtatc aaaattgatc atcacctgat
     6661 ttttatagta ttgataaaaa gcctcttcct cgccacggaa tccaaaacca ttaaatcggc
     6721 taaatacatc gcccttagtt tcataaaatt cacgcaccgg caagatttta cgcagttcat
     6781 ccaaagacga gcgcgaacgc atatactccc caaccgtgta aatatcatct tgcgcacggg
     6841 caaaacctgt gccctgcaaa atggcaccca ggccattgag agaagattgg cttttaggcg
     6901 agcgcaccac aaagctcgat tgcgacgtaa aacgatcgga agcgaagaag ccgaaataca
     6961 ccaacgaaat taccgtaggg ataatcaccg ttacccaaaa taaagggctt agctttttaa
     7021 tccaactttt ctttttcggc tttttacgct cggctttggt ttcagtggca actgccacag
     7081 gaagttgctc agacatctta attactcaca ttaattagtt aaattattaa tactgttcgc
     7141 gccactggta accggcgaga acacaaacga caagaatttc tgcacttcag ccaacggcgc
     7201 attcgacaca tacaatacat ctttattctt cacaggaaag cgctgcatag aaaatagcga
     7261 atgcgcatca gccatattca cacgatatac cgttggaatc tctgcctcac tgccataacc
     7321 ttgagcaatc catttatcct gacgttctgc cggcaattcc accaatggcg tatagcggaa
     7381 cacaaacaca ccacgcgcat cagaacggcg atcttgcaaa ccgcccatac ggccaatggc
     7441 ttcagaaagc gataagcctc tggctgaaaa accgatttct tgtgttctcc ccaccgcacc
     7501 catagacgta aaggtatagg gattggtaat catggtaacc acatcaccgc gacgcagcaa
     7561 aatattttgt cgcggatttg caactaaatc ttccaaggca acagttcgta ctacattgcc
     7621 acgtgtcagc tgcacattcg tatcctgcac atttgccgtt gaaccaccta ccgcagccac
     7681 cgcatccaac acacgctcac cggctgccgt cagcggcata cgcacactat tcccagcacg
     7741 aatcaccgac acattcgccg cattattctg caccaaacgc accatcactt gtggctgatt
     7801 ggccattttt ttcaggcggc ctttaataat ttcctgaacc tgaccaggcg ttttaccgac
     7861 caccgaaata tcgccaacaa acggcacaga aaccgtacca cgtgccgtga ccaactgctc
     7921 tggcaactta gtttgatgcg cactacccga gcccatcgaa gaaaggccac caccaaacaa
     7981 tactgccggc ggcgcttccc aaatcataat atccaataca tcaccaatat ttagcgtacc
     8041 agccgaagca taaccatcgc caaactgagt gaatgactga tttatctgag ccttatataa
     8101 taactgagca accgtatgat tcacatcaat cagctccact tcaggaattt gaacttcaga
     8161 ttgttgccct aaagagacaa ttttttttgc gctggggcct gatgaaggaa tcgcagagca
     8221 tcctacaatt aaacttccac acaataataa tactgcgtga cgaatataaa atttcacttt
     8281 aaacacaagc caaatcctaa tataattata aatggcctaa ttatagcact taatcgaaat
     8341 aaatttatga gtacgtagag tataattagt attcttcttt ccaacttcct tatacttata
     8401 tacttataga ttctaaaatc atgaaaagaa ttctttgcat tacaggtacc agagccgact
     8461 tcggcaagct aaaaccttta ttagcctata ttgaaaatca cccagacctt gaattgcatt
     8521 tgattgtaac tggtatgcat atgatgaaaa catatggcag aacctgcaag gaagtaactc
     8581 gagaaaacta tcaacataca tatctgtttt caaatcaaat ccaaggtgaa ccaatggatg
     8641 ccgttttagg caataccatt acgtttatct ctcgtctatc tgatgaaatt gaacctgata
     8701 tggtcatgat tcacggcgac cgtttagaag cactagcagg cgcaactgta ggtgcattaa
     8761 gcagccgttt agtttgccat atcgaaggtg gtgaactatc tggtacagta gatgactcca
     8821 ttcgtcattc tattagtaaa ctttctcata tccacttggt agcaaatgaa caagctgtca
     8881 ctcgcctagt gcaaatggga gaaaaaagaa agcatattca catcatcggc tcccccgatt
     8941 tagatgttat ggcctcttcc accctcccat ccttagaaga agtcaaagaa tattacggtt
     9001 taccatacga aaattatggt atttctatgt ttcaccccgt gactacagaa gcacatttaa
     9061 tgccacaata tgcggcccaa tatttcaaag cattagaatt aagtggccaa aatatcatta
     9121 gcatttaccc taacaacgac acgggtactg aaagtatttt acaagagtta ttaaaatgtc
     9181 aatctgaaaa atttatcgca tttccatcta tacgatttga atacttttta gtcttattga
     9241 aacatgctaa atttatggtc ggcaactcaa gtgcaggtat tagagaagct cctctctacg
     9301 gtgtcccttc aattgatgtt ggtacacgcc aaagtaaccg ccatatggga aaatctatta
     9361 ttcatacaga ttatgaaact aaaaatatct ttgatgcgat tcaacaagca tgcagtttag
     9421 gcaaatttga agcagatgat accttataat ggcggagata ctcgcaccag cacagaaaga
     9481 tttgctgaag taatcaacaa tcctgaaacg tggaatgttt ctgctcaaaa acgttttatc
     9541 gatttgaatc tttaaattat ggaaaaacaa aatattgcgg ttatacttgc gcgccaaaac
     9601 tccaaaggat tgccattaaa aaatctccgg aaaatgaatg gcatatcatt acttggtcat
     9661 acaattaatg ctgctatatc atcaaagtgt tttgaccgca taattgtttc gactgatggc
     9721 gggttaattg cagaagaagc taaaaatttc ggtgtcgaag tcgtcctacg ccctgcagag
     9781 ctggcctccg atacagccag ctctatttca ggtgtaatac atgctttaga aacaattggc
     9841 agtaattccg gcacagtaac cctattacaa ccaaccagtc cattacgcac aggggctcat
     9901 attcgtgaag ctttttctct atttgatgag aaaataaaag gatccgttgt ctctgcatgc
     9961 ccaatggagc atcatccact aaaaaccctg cttcaaatca ataatggcga atatgccccc
    10021 atgcgccatc taagcgattt ggagcagcct cgccaacaat tacctcaggc atttaggcct
    10081 aatggtgcaa tttacattaa tgatactgct tcactaattg caaataattg tttttttatc
    10141 gccccaacca aactttatat tatgtctcat caagactcta tcgatattga tactgagctt
    10201 gatttacaac aggcagaaaa cattcttaat cacaaggaaa gctaaatgca aaacaacaac
    10261 gaatttaaaa ttggtaatcg ttcagtaggt tacaaccacg aaccattgat tatctgtgaa
    10321 atcggcatca atcatgaagg ctctttaaaa acagcttttg aaatggttga tgctgcctat
    10381 aatgcaggcg ctgaagttgt taaacatcaa acacacatcg ttgaagacga aatgtctgat
    10441 gaggccaaac aagtcattcc aggcaatgca gatgtctcta tttatgaaat tatggaacgt
    10501 tgcgccctga atgaagaaga tgagattaaa ttaaaagaat acgtagagag taagggtatg
    10561 atttttatca gtactccttt ctctcgtgca gctgctttac gattacaacg tatggatatt
    10621 ccagcatata aaatcggctc tggcgaatgt aataactacc cattaattaa actggtggcc
    10681 tcttttggta agcctattat tctctctacc ggcatgaatt ctattgaaag catcaaaaag
    10741 tcggtagaaa ttattcgaga agcaggggta ccttatgctt tgcttcactg taccaacatc
    10801 tacccaaccc cttacgaaga tgttcgattg ggtggtatga acgatttatc tgaagccttt
    10861 ccagacgcaa tcattggcct gtctgaccat accttagata actatgcttg cttaggagca
    10921 gtagctttag gcggttcgat tttagagcgt cactttactg accgcatgga tcgcccaggt
    10981 ccggatattg tatgctctat gaatccggat acttttaaag agctcaagca aggcgctcat
    11041 gctttaaaat tggcacgcgg cggcaaaaaa gacacgatta tcgcgggaga aaagccaact
    11101 aaagatttcg cctttgcatc tgtcgtagca gataaagaca ttaaaaaagg agaactgttg
    11161 tccggagata acctatgggt taaacgccca ggcaatggag acttcagcgt caacgaatat
    11221 gaaacattat ttggtaaggt cgctgcttgc aatattcgca aaggtgctca aatcaaaaaa
    11281 actgatattg aataatgctt attaacttag ttactttatt aacagaggat tggctattac
    11341 atatagctaa ttctcattaa tttttaagag atacaataat gctaaagaaa ataaaaaaag
    11401 ctctttttca gcctaaaaag ttttttcaag attcaatgtg gttgacaaca tctccatttt
    11461 atcttacccc cccacgtaac aatttatttg tcatatctaa tttaggtcag cttaaccaag
    11521 tccaaagcct aattaaaata caaaaattaa ccaataattt actagtaatt ttatatactt
    11581 ctaaaaactt aaaaatgcct aagttagttc atcaatcagc taacaagaat ctatttgaat
    11641 ctatttatct atttgagctt cctagaagcc ctaataatat aactcctaaa aaattacttt
    11701 atatttatag aagttacaaa aaaatcctta atattataca gcctgctcat ctctatatgc
    11761 tgtcttttac aggccactac tcctatctga ttagtattgc aaaaaaaaag aatattacga
    11821 ctcatttaat tgatgaaggg actggaacat atgctccttt attagaatca ttttcatatc
    11881 atccaacaaa attagaacgt tatttgattg gaaataatct taatattaaa ggatatatag
    11941 atcattttga catattgcat gtcccctttc ctgaatatgc taaaaaaata tttaatgcaa
    12001 aaaaatataa ccggtttttt gcgcatgctg gaggaataag cattaataat aacattgcaa
    12061 acttacagaa aaaatatcaa atatctaaaa atgactatat ttttgttagt caacgctacc
    12121 ccatttcaga tgatttgtat tataagagta tagtagaaat cttaaacagc ataagtttac
    12181 aaattaaagg aaagatattt attaaactac acccaaaaga gatgggcaac aactatgtaa
    12241 tgtctttatt tctaaatatg gtagaaataa accctcggct ggtagttatt aatgaacctc
    12301 cttttctaat tgagccccta atatacttaa caaatcctaa aggaattata ggcctggcct
    12361 ctagttcttt aatttataca ccattactct caccctcaac ccaatgtctt tctattggag
    12421 agttaattat taacttaatt caaaaatatt caatggtgga aaacactgaa atgatccaag
    12481 aacacttaga gattattaag aaatttaatt ttattaatat actaaatgat ttaaatgggg
    12541 taataagtaa ccctctcttt aaaacagaag aaacatttga aacacttctt aaatctgcag
    12601 aattcgcata taaatctaaa aactactttc aggctatttt ttactggcaa cttgccagca
    12661 aaaacaatat taccttatta gggcataaag cattatggta ctacaatgca ctttataatg
    12721 taaaacaaat ttataagatg gaatattcag atatttttta tatcgataat atctccgtag
    12781 actttcatag taaagataaa ttgacatggg aaaaaattaa acattattac tattccgccg
    12841 acaatagaat tggtagagat agataaaaat actatgactt ctgatctcca tacgtatgct
    12901 tgttttcaaa aagcagcatt aagatagata agtgaagctt gatgagagcc atttttgtgg
    12961 tcatcgccaa ggtacacgcg gagagtaatc gacaacagag ctgctgtatt ctggctttta
    13021 aagcccagta gtaaagttta taaggctgag acctttgcaa aatcccccaa aatcccctaa
    13081 att
//
LOCUS       DAWGJV010000048        12786 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWGJV010000048 DAWGJV010000000
VERSION     DAWGJV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.048"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..1191)
                     /locus_tag="WHQ94_000982"
     CDS             complement(<1..1191)
                     /locus_tag="WHQ94_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ2128279.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNNDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQC"
     gene            complement(1224..3749)
                     /gene="ligA"
                     /locus_tag="WHQ94_000983"
     CDS             complement(1224..3749)
                     /gene="ligA"
                     /locus_tag="WHQ94_000983"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ2128280.1"
                     /translation="MNPNSKHNTNLTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     IQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYGHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQALIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(3897..5183)
                     /locus_tag="WHQ94_000984"
     CDS             complement(3897..5183)
                     /locus_tag="WHQ94_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ2128281.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVCDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5377..5949)
                     /gene="ampD"
                     /locus_tag="WHQ94_000985"
     CDS             complement(5377..5949)
                     /gene="ampD"
                     /locus_tag="WHQ94_000985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ2128282.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6033..7028
                     /gene="mltG"
                     /locus_tag="WHQ94_000986"
     CDS             6033..7028
                     /gene="mltG"
                     /locus_tag="WHQ94_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ2128283.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7088..7708
                     /locus_tag="WHQ94_000987"
     CDS             7088..7708
                     /locus_tag="WHQ94_000987"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ2128284.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            7930..9210
                     /locus_tag="WHQ94_000988"
     CDS             7930..9210
                     /locus_tag="WHQ94_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ2128285.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            9293..9563
                     /locus_tag="WHQ94_000989"
                     /pseudo
     CDS             9293..9563
                     /locus_tag="WHQ94_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9603..10637
                     /locus_tag="WHQ94_000990"
     CDS             9603..10637
                     /locus_tag="WHQ94_000990"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ2128286.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIAAALVSGLREKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10843..11424
                     /locus_tag="WHQ94_000991"
     CDS             10843..11424
                     /locus_tag="WHQ94_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ2128287.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11493..11675
                     /locus_tag="WHQ94_000992"
     CDS             11493..11675
                     /locus_tag="WHQ94_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ2128288.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            11672..12433
                     /gene="kdsB"
                     /locus_tag="WHQ94_000993"
     CDS             11672..12433
                     /gene="kdsB"
                     /locus_tag="WHQ94_000993"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ2128289.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            12456..>12786
                     /locus_tag="WHQ94_000994"
     CDS             12456..>12786
                     /locus_tag="WHQ94_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ2128290.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EASGEFWFRMAETLSTLPPNRSLDLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKIT
                     ALNYSGLN"
BASE COUNT         2826 a         3218 c         3771 g         2971 t
ORIGIN      
        1 acattgcagc aaatcgttta aaaacaagcg tcctttttcg gtcgggcgga atacggcggg
       61 gtcggtttcc agcaggcctt tttgccttgc cgtttcgatt tgcgccatga ttttggcact
      121 cggtacgccc gtgcgctcct gcaacatcgc ggcgggtacg ccgtcggtca ggcgcagggc
      181 gttcatcata aactcgaacg gcaaatcttc ggcggcaacg gttttgcgtt cgacggcttc
      241 actcggttgg ctttgcatta aggcgaggta gtcgttgggg tggcggcggc ggacggtgcg
      301 ctcgatgcgg tcgggatagg aaattttgcc gtgagcgccc gcgcctatgc ctaaataatc
      361 gccgaactgc cagtagttca aattgtggcg gcactgcatg gctggtttcg caaaagccga
      421 tgtttcgtag tggacaaaac ccgcgccttc cagcgcaccg tgtaccgcgt cttcgatgtc
      481 gagcgccgct tcgtcttgcg gcaaaccttt cggcggcgta tgaccgaacg gcgtgttcgg
      541 ctccatcgtc agatgatacg cgctgatgtg ggtcgcaccc gtagcgatgg cggtttgcac
      601 gtcgtccaat gccgtctgaa cggtctggtt cggcagggca tacatcaagt cgatattgac
      661 tttatcaaat aatttcaagg cggtgtcgat ggcggttaag gcttctttgc cgttgtggac
      721 gcgccccagc cttgagagca tatcgttgtt gaaactctgc acgccaatag aaagccgcgt
      781 aatacctgcg tctttaaatc cttgaaactt ctcgatttca aaggtgcctg gattggcttc
      841 caaggtaatt tccgcctccg gctgcaagcg caacagtgaa cgcacgccgc tcaacaaacg
      901 gtcaatcgat tccgcctgaa acaggctggg cgtaccgcct ccgaaaaaga tcgtttccac
      961 cggcctgccc caaatattgg gcaattcaag ctgcaagtcg gtcagcagcg cgtcgatata
     1021 ggcggcttcg ggcaatccgt ttttcaggct gtgggaatta aagtcgcaat acgggcattt
     1081 tttgatgcac cacgggatgt ggatatagag tgacaggggc ggcagggcgg tgagccgggt
     1141 gcggtttgga aaggaaatgg tgtgcatggt gtggttcgga aaagtggaca atgccgtctg
     1201 aaggcggttc agacggcatg ggttcagccg agcagggtaa gcagttcggc ttcgctgagg
     1261 acggaaacgc ccaaggcatt ggctttttcc agcttgctgc ccgcggcttc tccggcgacg
     1321 acgtaatcgg tttttttgga aacgctgccg gaaactttgc cgcctgcggc ttcgattagg
     1381 gcttgggctt ggtcgcgttt gagcgtgggc agcgtgccgg ttaatacaaa ggttttgcct
     1441 gctacggctt tattgatgcc gtctgaacct tgcgccgcct cgtcttcaga cggcatttgc
     1501 gcgaagaagg tttttaggtt ttcgagcagg gcggcgtttt gtggttcgct gcgccatgct
     1561 tgccagtcgg cggggagggc tttgtcgttt tgtagacctt ctatgttttt accagcgagt
     1621 tcccataagg cttgggcttt gttttcgctg attttgaaac cgggcaggcg ggcgatccag
     1681 cgttgcggtt cggcatattg tgcggcaggc aagctgacgg cttgggcttg cggggcaacg
     1741 cctgcggcga gcagctcgtc tatcatcgcc tgctgttcgg cttgggcgaa gaagtgggca
     1801 atggaacgcg ccactaccgt accgatgtcg ggcaggcagg cgaggacggg ttcgggggcg
     1861 cggcggacgc gttccaatgt gccgaatgcc tgtgccagcg ttttggcggt gcgttcgccg
     1921 acgtggcgga tgccgagcgc gaacaggaag cgcgcgagtt cgggcgtttt gccggattct
     1981 atgcctgcga ggatgttttc cgcccacttg gtcggttgtt ttttatgttt gcccgacgcg
     2041 ccgaccgaac tgccttcaga cggcatttga tccgattcgg caacggtttt gtccgccgtt
     2101 tccttcattt tttgcagggt cggaatatcg aggcggtaga ggtcggcgaa gtggcggacg
     2161 aggtcttggg cgacaagctg ttcgatttgt ttttcaccca agccgtcgat gtccatcgct
     2221 ttgcgcgagg cgaagtggat taagccttgc gcgcgttgtg cctgacaaag catgccgccg
     2281 ctgcatcggg cgacggcttc accttcttcg cgttcgattt cgctgcggca gatggggcaa
     2341 tgggtcggca ggcggtaggg cttgtagagc ggaacggatt gggtttggtt tgcagccggt
     2401 gcttcggcaa acaaatcgtc ctgccgatgc ccgatgccgt ctgaaacagc aacggcggtt
     2461 tcctgcatcg ggcggcgttc aaaaatcaca cgcacgactt cgggaatcac gtctccagcg
     2521 cggcgcacga cgacggtatc gccgacgcgc acgtctttac gcgatacttc gtcctgattg
     2581 tgcagggtgg cgttggtaac ggttacgccg ccgacgaata cgggttgtaa tcgggcaacc
     2641 ggcgttaccg cgcccgtcct gccgatttgc acgtcaatcg cttcgacaat ggtcagggct
     2701 tcttcggcag ggaatttgtg ggcaaccgcc caacgcggcg cgcgggagat gaagccgagt
     2761 tcgtgctgtt gcgccaagct gttgactttg acgaccatgc cgtcgatttc gtagggcagt
     2821 tcggggcgtt tttgctgcat gtgtccgtaa aacgccaata cttcgtcgat atttttgaaa
     2881 cagccgaaat tgccattggg cagactgaag ccgagtgctt ggaaataggc gagttcttgg
     2941 atgtgttctt ccgcgacgaa accgccttgc tggcgggcaa cggagtaggg gaaaaagtgc
     3001 agtttgcgct gcgcggtgat gcgcgaatcg agttggcgta ggctgccggc ggcggcgttg
     3061 cgcggattgg caaagggttt ttgcccgttt tcggcttgtc ttttattgag ggcggcaaaa
     3121 tcggcttttt gcatcagtac ttcgccgcgt acttcgatga gttcgggcgt attttcgccg
     3181 tgcagccgca aggggatgtt ggatacggtt ttgatgtttt gggtaacgtc ttcgcccgtc
     3241 gtgccgtcgc cgcgcgttgc tgcttgaatc aatacgccgt cgcgatagag caggctgatg
     3301 gcgaggccgt cgaatttagg ttcgataacg tattcgggat tgccgccgtc caaaccgtcg
     3361 cgcacgcgtt ggtcgaaagc gtacatttcg gcatggtcga acacgccgtt ttcatcttgc
     3421 ggggaaaaag cgttggtcag cgacagcatc ggcacttcgt ggcgcacttc ggcaaagcct
     3481 gccaaaggct cgccgccgac gcgctgggtc gggctgtcgg gcagtttgag ctcgggatgg
     3541 tttcgttcca acgcttcgag ttcacggaat aatttgtcgt attcggcatc gggtacgctg
     3601 ggcgcgtcga gggtgtagta ttcgtaggcg tagcggttga gcaggtcggt gaggtggcgg
     3661 atgtgttgtg cggcaaattg ttttatatca ctatcagacg gtttaagaag attggtaagg
     3721 ttagtgttat gttttgagtt tggattcatg agagaaggtt ttcagacgac ctttgtctga
     3781 tacgggatga aacgggcaaa ggtcgtctga aaaatgatag gttgaaaaca gctgaatttt
     3841 acccgaaaaa aagcggatat gccgtaacga catatccgct ttgattgcat tcgattttag
     3901 gagaacaggc gcaatgcggt tttgccgccc ggttcgatac cgactttgag catctcggac
     3961 tgacgagcca atacataagt gcgtacgtct ttgagccatt gggttgaaac ttcttccatt
     4021 ttgtcgttga ccagattcag gttcaactgg ccggacaggc gtaccgccaa atccataaac
     4081 aaatcgtcga aggttttttc gcctgccgga gagtgcggaa tatcgagcag catactgaag
     4141 cctttatagg attggttgtc caaaagggca ttggtaaacg gctcgttgtt gagcgagcag
     4201 atggagaaca tggtcgagcc cgacgtgtcg gtatagtgga acgcgccgtc gtcttccagg
     4261 acgaaaccca cgcccgttac ggcggaacgc agttctacgc cgctgatgct ggtcggggaa
     4321 accaaatgga tggcgatggt ctggtcgacg cgcgcgcaga atgcgtccag tgcggaagcc
     4381 acttcgataa aggcggcaag gtcggtgtgc agcgtctgac cgcccatgct ttgtgcgaat
     4441 gcgtccacct ggcggttgaa tgcggagagt tcttcctgcg aggcaagtcc gttgcggctg
     4501 actgcctgaa tacccacgat aaatgcctga tagcggatgc ccgggatggg ttcggcaatc
     4561 tggaaatggt cgtccatggt gcagccgaca atctggtagc ggcagcggtt ggaaaggcgc
     4621 ggcagtgcgt gcagttcttt ggcttcggtc agcgcgatat aggagatgaa gtcgaagcgc
     4681 acgtcaaacc agggtaattc gacttttgac agttctttga gcgtaatcag cggttttgca
     4741 ggtgtttgcg gaacgggtgc aggttttgcc ggcgcgtcgg caggtttcgg tgcggaatgt
     4801 ccggtttggg gttcggaaac ggtgtgggcg gagttgccga taatgccgct ttcttccaag
     4861 gcggtttcga tttcggtttt gaacggggag gcttttgcct gtttctgctt ggcgatgtag
     4921 acggcatcct gttcttgcag gttgcgcatg gcggggtctt ggggttttgc cgtttttttg
     4981 accgccggtt ggggtttcgg catcatgact gacccgccgg acggtttgcc gtcgcggaca
     5041 tggctggttt tgctgttgag cagggcatct ttgtcggagt gtccgaactg gtcgcacact
     5101 tttttgcggt attggttttc ctgatacata ttgtaggcga caacggcgag gacggcggcg
     5161 aggaacagta cgatgtaaat catggcaatc actttaaatt tcgggatgca ggatgcgcaa
     5221 agtgcgggta ctgcggttaa atcgggcttg cgtttccggc agtctgacgg aacggccggt
     5281 tataacgttt gaattataac gaaaattgca gggtctgaca gcagtgtgtc gaaataagcg
     5341 gaaattttcc gaaatgccat ctaaaatctg tggttttcag acggcatttc tgtccacggg
     5401 aaaccctttc ccccgtatcc gccgccagtc gaaaaaatga ccggggtcgg ttttgcggcc
     5461 gggcgcgatg tcttggtgcc ccgttaccgc cgtgatgggg tagcggcggc agattgcgtc
     5521 caacaaggct tcgagcgaac ggtattgcgc ttcggtaaag ggttcgaaat cgcagccttc
     5581 cagttcgatg ccgatggaaa atgcgttgca tttttcccgt ccgcgaaacg aggatacgcc
     5641 ggcatggtat gccatattgt cgcaggaaac gaactgtacc gtttcgccgt cgcgtttgat
     5701 taaaaaatgg ctggatacgc gcaaagtgtg tatcaggctg aagaacggat gtccgtcggg
     5761 gtcgagccgg ttggcaaaca gcttttccac cgcatccgta ccatattcga acggcggcag
     5821 cgaaatgttg tgcaacacga tcagggaaac cgtttccccc gtttccctcg ggctgaaatt
     5881 gggcgacggg gtatggcgta tgctttgaag ccagccgttt tgccagtgtg cttcggcgtg
     5941 attgtccatg atgttcttcc tgtccggcgg gcaatttggg ttatactgtc gcccgaattt
     6001 taagacgtat tccgaatgct gggaatccta ccatgttgag aaaattgttg aaatggtctg
     6061 ccgttttttt gaccgtatcg gcagccgttt tcgccgcgct gcttttcgtc cctaaagaca
     6121 acggcagggc atacaggatt aaaattgcca aaaaccaggg tatttcgtcg gtcggcagga
     6181 aacttgccga agaccgcatc gtgttcagca ggcatgtttt gacggcggcg gcctacgttt
     6241 tgggtgtgca caacaggctg catacgggga cgtacagact gccttcggaa gtgtctgctt
     6301 gggatatctt gcagaaaatg cgcggcggca ggccggattc cgttaccgtg cagattatcg
     6361 aaggttcgcg tttttcgcat atgaggaaag tcatcgacgc aacgcccgac atcgaacacg
     6421 acaccaaagg ctggagcaat gaaaaactga tggcggaagt tgcccctgat gccttcagcg
     6481 gcaatcctga agggcagttt ttccccgaca gctacgaaat cgatgcgggc ggcagcgatt
     6541 tacggattta ccaaatcgcc tacaaggcga tgcaacgccg actgaatgag gcatgggaaa
     6601 gcaggcagga cgggctgcct tataaaaacc cttatgaaat gctgattatg gcgagcctga
     6661 tcgaaaagga aacagggcat gaagccgacc gcgaccatgt cgcttccgtc ttcgtcaacc
     6721 gcctgaaaat cggtatgcgc ctgcaaaccg acccgtccgt gatttacggc atgggtgcgg
     6781 catacaaggg caaaatccgt aaagccgacc tgcgccgcga cacgccgtac aacacctaca
     6841 cgcgtggcgg tctgccgcca accccgatcg cgctgcccgg caaggcggca ctcgatgccg
     6901 ccgcccatcc gtccggtgaa aaatacctgt atttcgtgtc caaaatggac ggtacgggct
     6961 tgagccagtt cagccatgat ttgaccgaac acaacgccgc cgttcgcaaa tatattttga
     7021 aaaaataaac catgccgtct gaaaagtttg tgttttcgga cggcataccc ttaccggaac
     7081 tgcaagcatg aaaccgcaat tcatcacttt ggacggcata gacggtgccg gcaaatccac
     7141 caaccttgcc gtcatcaagg catggtttga acggaggggg ctgcccgtgc tgttcacgcg
     7201 cgaaccgggc ggaacgccgg tcggtgaggc cttgcgcgaa atcctgctca accctgaaac
     7261 caaagccggt ttgcgtgcgg aaacactgat gatgttcgcc gccagaatgc agcatattga
     7321 agacgtgata ttgcctgcac tttcagacgg catccatgtc gtgtccgacc gttttaccga
     7381 cgcaaccttc gcctatcagg gcggcgggcg ggggatgccg tctgaagaca ttgaaatttt
     7441 ggaacattgg gtgcagggcg gtttgcgccc cgatttgacc ctgctgttgg atgtgccgct
     7501 ggaagtgtcg atggcgcgta tcggacagtc gcgcgagaaa gaccggttcg agcaggaaca
     7561 ggcagatttc ttcatgcgtg tgcgcagcgt ttatctgaac cgtgccgccg cctgtcccga
     7621 acggtacgcc attatcgaca gtaaccgcag cttggatgaa gtcagaaaca acatagaaaa
     7681 agtgttggac ggacatttcg gctgttaatg cggcaaatat tgaaacaagc gcatccaccc
     7741 gcgccgaaaa tcaaacggca gtgccgcagg tgaaaatggc ggtatgcgcc aaactttcgg
     7801 catgatagaa ttacgctcgg ttacaaggca ggatgcgtcg gcaatattaa cgaaccgccc
     7861 gtaacatgat gacccgaaag cgtttcggac agtccgattc aaatcttttt ctcgcaacag
     7921 gattgacaca tggaaaactc attgaaagaa gccgccctca agttccacga attacccgtt
     7981 ccgggcaaaa tttccgttac cccgaccaaa tctttggcga ccgacaaaga tttggcgttg
     8041 gcgtactctc cgggcgtagc cgctccttgt atggaaatcc atgccgatcc gcaaaatgcc
     8101 tacaaataca ccgccaaagg caacttggtc gccgtcattt ccaacggtac ggccgttttg
     8161 ggcttgggcg acatcggcgc gctggcgggc aaacccgtga tggaaggcaa aggcgtattg
     8221 ttcaaaaaat tcgccggtgt ggacgtgttc gacatcgaaa tcgatgaaaa agacccgcaa
     8281 aaactcgtgg acatcatcgc cgctttagag ccgaccttcg gcggcatcaa cctcgaagac
     8341 atcaaagcac ccgagtgttt ctacatcgaa cgcgaattac gcaaacgctg caaaatcccc
     8401 gtattccacg acgaccagca cggcacggcc atcattaccg ccgctgccgt attgaacgcc
     8461 ctgcgtttta ccggccgtaa aatcgaagaa gcgactttgg tgtgttccgg cgcaggtgcg
     8521 gccgcgattg cctgcttgaa ccaattgctg gatttgggct tgaaacgcga aaacgtgacc
     8581 gtttgcgact ccaaaggcgt gatttaccaa acccgcgaag acaaagatcg catggacgaa
     8641 tccaaaaaat tctacgccgt tgaagacaat ggccagcgcg tacttgccga tgccgtcaaa
     8701 ggtaaagaca tcttcttggg cctctccggc gcgaacctgc tgacccccga aatgttgaac
     8761 acgatgaacg aaaaacccat cgtgttcgcc atggccaacc cgaatccgga aatcctgccg
     8821 ccgctggcaa aagaaacccg tccggacgtg gttatcggta ccggccgttc cgacttcccg
     8881 aaccaagtga acaacgtatt gtgcttccca ttcatcttcc gtggcgcgtt ggatgtcggc
     8941 gcgacgacca tcaacgaaga aatgaaacgc tcctgcgtgt atgctttggc ggatttggcg
     9001 atggaagaag taaccgaaga agtggttgcc gcttacggta agaaatttga attcggcgcg
     9061 gaatacctga ttccgactcc gttcgattcc cgcctgctgc cgcgcgttgc tacggctgcc
     9121 gccaaagcag cgatggaaag cggcgtggca acccgtccga ttgccgactt tgaagcctac
     9181 gccgccaagc tgggcgaatg gaagctgtaa gccgtttgcg gtttaaaatg ccgtctgaac
     9241 tgttttcagg cggcattttg ctgtcagatt gataaatgaa agatactgga aaatgaaaga
     9301 gatggagact gtccgttatc atatcggcga tatgcccgag acttcaaaac aaaccgcccc
     9361 tgtcatgacg acagggcagt gggtgttgac gatgattgtt ttcatgattc ctttggtcaa
     9421 tttttgtttg ggtgttcggc agaggcaacc cgaaccgcgc caatttctgt aaagcgcagt
     9481 tgcttattta cctgattggt tcgcttatcg gtttggtctt cgcgttgttt ataggtgggt
     9541 ctgtatcggg tacgcatgat taatgccccg ggctgatttt gcttcgagga tttgtatcga
     9601 atatgccgaa ttgtttcaaa tttcataccg ttatcgaacg gcattggcaa aaaccttatc
     9661 cggttttgtc ttttctgctt aagccgctct ccgggctgtt tgccaaaatt gcggcaaaac
     9721 ggcggacgga ttttttatcg ggaaaacggc aaagcgaaaa gctgcccgtg cctgtggtcg
     9781 tggtcggcaa catccacgcg ggcgggacgg ggaaaacgcc gattgccgcc gcgctggtgt
     9841 cgggtttgcg ggaaaaaggt gtcaaggtcg gcatcatcag ccgaggctac gggcgcaaga
     9901 gcaaggcggt tcatgtattg aatgccgcga gccgtgcgga agatgcgggc gacgagcctt
     9961 tgctgctgtt cagcaaaacc ggtgcgccga cggcggtggg cagcagccgt gcagaggcag
    10021 gcagggcgtt gctggcggcg catcccgaca tcggactgat tgtggcggac gacggtttgc
    10081 agcattacgc cctgcggcga gatgtggaaa tcgcggtgtt tccggcggcg gatacggggc
    10141 gcacggattt ggatttgctg cccaacggca atttgcgcga acctttgtcg cggctggatt
    10201 cggtggatac ggtcgtcgtc agcggcggca aggcggatgc gctgtttagg ccgtctgaaa
    10261 atatgtttca cagccgtatc gaagcgggac gaatttaccg tttgaacaat ccgtccgaaa
    10321 tactggacac aggccgtctg aaaaatcaaa ccgtcgtcgc cgtggcaggt attgccaagc
    10381 cggcgcgttt tttcgattcg ttgcggaata tgggcattac cgtgaagcga accgtcgcgc
    10441 tgcccgacca cgccgacatt tcggcggcag atttgcccga tgcggacgcg gtcattatta
    10501 cggagaaaga tgcggtcaaa ttttcagacg gcatttgcac cgataatgtt tgggtgttgc
    10561 ccgtttgtgc gataatcgaa cctgatttgg cggcgtttgt gttggagcgg ttggaagatg
    10621 taccgaaggc cgtctgaaag cacggtttgg gcggagtgat tacggatttg aataagaacg
    10681 cctcgcgccg tcattcccgc gcaggcggga atctaagtct cgaattttca ggaatgccta
    10741 ggaggcttca gaaatcccaa atctccggat ttccacttgg acaggaatga gaaaatcggt
    10801 cgtatttttt atctgcatta atcattcgtt aaaggattga atattaaact gaaaaccttg
    10861 ttattgccct tcgccacgct ggcattgtgc accaacgctt ttgccgcccc gcccagcgac
    10921 gcgtcgttgg cgcgttggct ggatacgcag aattttgacc gggatataga aaaaaatatg
    10981 attgagggct ttaatgccgg atttaaaccg tatgcggaca aagcccttgc cgaaatgccg
    11041 gaagcgaaaa aagatcaggc ggcagaagcc tttaaccgtt atcgtgagaa tgttttgaaa
    11101 gatttgatta cgcccgaagt gaaacaggct gtccgcaata ctttattgaa gaatgcccgt
    11161 gagatataca cgcaagaaga aattgacggc atgattgcct tttacggttc gcctgtcggt
    11221 cagtccgtcg ttgccaaaaa tccgcgctta atcaagaaat cgatgagtga aatagcagta
    11281 tcttggactg cattgtcagg gaaaatcgcg cgacatcatc tgcccgcgtt tacggaagag
    11341 ttgcggcgca tcatctgcgg cggtaaaaat cccgatgcgg gctgtaaaca agccggacag
    11401 gttgggaaaa ggcatcagaa ataaatgata gccgtctgaa atattgaaga gggcatccga
    11461 ttgattgaac catcaaaccc gaaagcaacc ctatggaaaa aaaattctta gacatcctcg
    11521 tctgccccgt taccaaaggc aggctggaat atcatcagga caaacaggaa ttgtggagcc
    11581 gtcaggcgaa gctggcctat ccgattaaag acggcattcc ctatatgctg gaaaacgaag
    11641 cgcgtccgtt gggcgaagag gaactcgaag catgaccgaa ttcgtcgtat tgattccggc
    11701 gcggctggat tcgtcgcgcc tgcccggaaa agccttggcg gacatccacg gcaaaccgat
    11761 ggtcgtgcgc gttgccgaac aggcggcaaa aagtaaagct gcgcgcgtcg ttgttgccac
    11821 cgaccatccc gatattcaga cggtctgtca ggcgcacggt atcgaagtcg tcatgacttc
    11881 aaaccggcac gaaagcggca cgacgcgcct tgctgaagcc gccgccgcgc tgaagctgcc
    11941 gccgcatctg gtggttgtga acgtacaggg tgacgagccg ctgattgccc ccgaactcat
    12001 cgaccgcacc gccgaagtac tcgtcgaaaa caacgtccaa atggcgaccg ccgcccacga
    12061 attgcacgat ttcgacgaat tgatgaatcc caatgccgtc aaagtcgtcc tcgacaaaaa
    12121 ccgcaacgcc atctacttca gccgcgcccc gattccctat ctgcgcgatg caatgcgtgc
    12181 cggaaaacgc gaaatgccgt ctgaaaccgc cgtcctgcga catatcggca tctacgctta
    12241 ccgcgccggc ttcctgcaac gctatgccga aatgagcgtt tcgccgctgg aaaccatcga
    12301 atcgctggaa cagctgcgcg tcctgtggca cggttatccc attgccgtcg aaaccgccaa
    12361 agaagccccc gccgccggtg tggatacgca ggaagatttg gacagggtcc gcgccgtatt
    12421 tcagaccgta taaaacaggt tcaaagggaa aagatatgca gcaacatatt gaaaagtggc
    12481 agcacttgag ccgtgaagaa cagaaaatcc ttgctgaagt atggggtctc gtgcaaaacg
    12541 acgatcagga ggttcactat gaaatgctca aattgaacgc acccgacgaa gccagtggcg
    12601 aattttggtt cagaatggca gaaacactca gcaccctgcc gcccaaccgt tccctcgacc
    12661 ttagaatgaa cggcggcagg ctggcgaccg ccgtatccat cctttccgtc atgattgaag
    12721 acaatcccga cataccgcag ctttgggcgc aaaaaattac cgcgctcaat tatagtggat
    12781 taaatt
//
LOCUS       DAWGJV010000049        12370 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWGJV010000049 DAWGJV010000000
VERSION     DAWGJV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.049"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            200..1876
                     /gene="ettA"
                     /locus_tag="WHQ94_000995"
     CDS             200..1876
                     /gene="ettA"
                     /locus_tag="WHQ94_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ2128291.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGNLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            complement(1936..2421)
                     /locus_tag="WHQ94_000996"
     CDS             complement(1936..2421)
                     /locus_tag="WHQ94_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ2128292.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(2414..3370)
                     /gene="thiL"
                     /locus_tag="WHQ94_000997"
     CDS             complement(2414..3370)
                     /gene="thiL"
                     /locus_tag="WHQ94_000997"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ2128293.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            complement(4239..4967)
                     /gene="rmpM"
                     /locus_tag="WHQ94_000998"
     CDS             complement(4239..4967)
                     /gene="rmpM"
                     /locus_tag="WHQ94_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ2128294.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            5331..6281
                     /locus_tag="WHQ94_000999"
     CDS             5331..6281
                     /locus_tag="WHQ94_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ2128295.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(6394..7128)
                     /gene="fnr"
                     /locus_tag="WHQ94_001000"
     CDS             complement(6394..7128)
                     /gene="fnr"
                     /locus_tag="WHQ94_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ2128296.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            7333..8754
                     /gene="hemN"
                     /locus_tag="WHQ94_001001"
     CDS             7333..8754
                     /gene="hemN"
                     /locus_tag="WHQ94_001001"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ2128297.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(9107..10681)
                     /locus_tag="WHQ94_001002"
     CDS             complement(9107..10681)
                     /locus_tag="WHQ94_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ2128298.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            11006..12106
                     /locus_tag="WHQ94_001003"
     CDS             11006..12106
                     /locus_tag="WHQ94_001003"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ2128299.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
     gene            12225..>12370
                     /locus_tag="WHQ94_001004"
     CDS             12225..>12370
                     /locus_tag="WHQ94_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ2128300.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEM"
BASE COUNT         3201 a         3465 c         3013 g         2691 t
ORIGIN      
        1 ctttggaaaa tccgaaatgg ttgccggggc ggcgatcccc tatcattatt atttttttgt
       61 ctatataatt tcaaagggat aagcggattt tatgaatcct gcccgatttt ggcaataccg
      121 gttcgcggat aaactggctt aaatcaaatt atcggttaaa atggccgtct gaaatttgtt
      181 tgatgaaaac gagaaaacca tgtcccaaca atacgtctat tctatgctgc gcgtgagcaa
      241 ggttgtgccg ccgcagaaaa ccatcattaa agatatttcc ctttctttct tccccggcgc
      301 gaaaatcggc ctgctcggtt tgaacggcgc gggcaagtcc accgtgctgc ggattatggc
      361 gggcgtggat aaggaatttg agggcgaagc cgtgccgatg ggcggcatca aaatcggcta
      421 cctgccgcaa gagcctgagc ttgatccgga aaaaaccgtg cgcgaggaag tggaaagcgg
      481 tttgggcgaa gtggctgccg cgcagaaacg tttggaggaa gtgtatgccg agtacgccaa
      541 tcctgatgcg gattttgacg cgttggcaga agagcagggc cgcttggaag cgattattgc
      601 ggcaggttcg tccacgggcg gcggtgcgga acacgaattg gaaatcgccg ccgatgcgct
      661 gcgcctgccg gaatgggatg ccaaaatcga taatttgtcc ggcggtgaaa aacgccgcgt
      721 tgccttgtgc aaactcttgt tgagcaagcc cgatatgctt ttgctggacg agccgaccaa
      781 ccacttggat gcggaatcgg tcgaatggct ggagcaattt ctcgtgcgct tccccggcac
      841 agtcgttgcg gtaacgcacg accgctactt cctcgacaat gccgccgaat ggattttgga
      901 actcgaccgc ggccatggta ttccgtggaa aggcaattac tcgtcttggc tggagcagaa
      961 agaaaaacgc ttggaaaacg aggcaaaatc cgaagccgcg cgcgtgaagg cgatgaagca
     1021 ggaattggaa tgggtgcgcc aaaatgccaa aggccgccaa gccaagtcca aagcgcgttt
     1081 ggctcgtttt gaagaaatga gcaactacga ataccaaaaa cgcaatgaaa cgcaggaaat
     1141 cttcattccc gtcgccgagc gtttgggtaa cgaagtgatt gaatttgtga atgtttccaa
     1201 atcgttcggc gacaaagtgc tgattgacga tttgagcttc aaagtgcctg cgggcgcgat
     1261 tgtcggcatc atcggtccga acggcgcggg taaatcgaca ctgtttaaaa tgattgcggg
     1321 caaagagcag cccgattccg gtgaagtgaa aatcgggcaa accgtgaaaa tgagcttgat
     1381 tgaccaaagc cgcgaaggtt tgcaaaacga caaaaccgtg ttcgacaaca ttgccgaagg
     1441 tcgcgatatt ttacaggttg gtcagtttga aattcccgcc cgccaatatt tgggacgctt
     1501 caatttcaaa ggcagcgacc aaagcaaaat cgccggcaac ctttcaggtg gcgaacgcgg
     1561 tcgtctgcac ttggcgaaaa ccttgttggg cggcggcaat gtattgctgt tggacgaacc
     1621 gtccaatgac cttgacgtag aaaccctgcg cgcgctggaa gacgcattgc tggaatttgc
     1681 cggtagcgtg atggtgattt cgcacgaccg ttggttcctc gaccgtattg ctacgcatat
     1741 cttggcttgc gaaggcgact ccaaatgggt gttcttcgac ggcaactatc aggaatacga
     1801 agccgacaag aaacgccgac tcggcgaaga aggcgcgaaa ccgaaacgca tcaaatacaa
     1861 accggtaacg cgttaacctc cgaaacaatg ccgtctgaaa ggctttcagg cggcattttt
     1921 acaaggcagc accgtttaaa acagcattgc aatcctcaag acaatcaaag tcatcaccgc
     1981 agccgccata tcgtccgcca taatgcccaa accgccgtgc agattcttgt caaaccaacc
     2041 gacgggagac ggtttgagcg cgtcaaacag acggaatagg acaaatgccg ccagccacca
     2101 cgtccacctg aacggcacaa acgccagcac aaacagcatg gcgacaatct cgtcccaaac
     2161 aatcccacca tggtcgctga cacccgtttc acgttccgca taagcgcaaa tgcgtatgcc
     2221 ccacataaac agcacgatac acaaaaaagc cagcagcagc ccgtctatgc cgagcaaaat
     2281 cagcacaaat gccaaaggca gtgccgccaa agtgccaaat gtacccggcg cgaacggagc
     2341 cagcccgctg ccgaaaccaa aagccaaaaa acacaacggt cgtttcaaca gccacgcaaa
     2401 gtcaggttta aaatcagcca aaatgatcga atcctaaaga atgtagttcc aattccctgc
     2461 cgtcggcatc taaaaccttc agacggcatc ctccgttgat tttgccgatg cgcgttaccg
     2521 gcacgccgca ccgttctgcc gcatcaagca cgcggctgcg gcaactttcc ggcgcggtaa
     2581 acaccagctc gtaatcgtcg ccgcccgcca aagtataaga cagccattgc gctcggggca
     2641 aaatatcttt caatacggat aaagacggca gcgaatcggc ccaaatttcc gcccccacgc
     2701 cggaagcggt caggatatgc cccaaatctt gcgcgaggcc gtctgaaaca tcctgcgccg
     2761 cccgggcaaa cggcaacagc gcaagcccca gcccaaccct tggctcgggg cggagcagct
     2821 tttgttcgca ttcagcaaac acatcatccg gcaacacaca ccgtttcaga cggcagttca
     2881 aagccgccgc cgccataccg acacgccccg acacccaaat atcgtcgccc gcaaccgccg
     2941 catcacgccg caacgcccta cccttcggca attcgccgat aatggttaca ttgaacgcca
     3001 tatcgccctt ggtcgtatcg ccgccgatta acgttacgcc aaactttttt gccaaaccga
     3061 aaaagctgcc gcaaaaccgt ttcagccata cctcatccaa ttcgggcaac gccgcgctca
     3121 gcaacaccca acgcggtatc gcacccatcg ccgccatatc cgaaacattg acggccaaaa
     3181 ccttccaagc caagtcttca ggtttgacat ttgcaaaaaa atgcctgtcc ttcaaaagca
     3241 tatccgcact gaaacacaaa tcgaagcctt cacgcgggcg gacaatcgcc gcatcgtcgc
     3301 ctatgcccaa tacgacatca ttatccgtgc ctgtttgcaa ataccgtttg atgaagtcaa
     3361 attctttcat atcccaaaat atccggcggg cgaatccaat ttaaatcata gcaaggcagc
     3421 aaaggccgta caaacaataa agcggctcat tcagtgtttc agcgtatttc acttttagct
     3481 ttggccatac cgtacccgta tgcaccgacc accctgtttc aacacacaaa cggcatatca
     3541 aagcaccgca ctgcacgcca ccccgaatgc aaagcggcaa cttccgccac cgattaggct
     3601 caatcgctgc aactgacggg gtttcaaccg aaaagggctg atgataaaac catatccggc
     3661 atacagtgcc gcacacatct ggcacgattc catccatttc ccaatgaagg ggaaaggcgt
     3721 gaatttgtat gaattatccc tttcagggct tgatacagaa aggattccct ttctatgggc
     3781 aaacccacat cctaccgcct taaaatcaga gtttcaaccg atatagtgga ttaaatttaa
     3841 accagtacag cgttgcctta ccttagctca aagagaacga ttctctaagg tgctgaagca
     3901 ccaagtgaat cggttccgta ctatttgtac tatctgcggc ttcgtcgcct tgtcctgatt
     3961 tttgttaatc cactataaag cacggcaatg ggacacaaac ctgaatctga atctgaatct
     4021 gaatctgaat ctgaatctga atctaaatct gaatccgaat ccgaacctgc acctcgattc
     4081 tgacgatagc cgtttcagaa aaccacccaa aaaaacaaac cccgcaaaag cgggggtttg
     4141 tttcacatca tcgatgccga ccgacgtttc acgaatattg caacagtacc tggtacaaaa
     4201 tccactaacc tcatgcatcg gcaagatatt gcctagcctt agtgttggtg atgattgtgt
     4261 gccggcacaa cctgacgggt tacgatgcta cggattttca catccacacg gcggtcaggt
     4321 tcgatacatg caatcagagc ctcacgtttt ttggctttag agactttcgc acccagtttg
     4381 gcaacttcgg cttcacaaac ttgagtcatt tgcgcttgag attcgcccaa gccgacagca
     4441 gaaattctag aaacaggtac gccgttgctg accaggttgt ttgccactac gtatgcgcgg
     4501 cgttcggaca gggcctgatt gtatttgtca gaacccataa agtcggtatg gccttcgacg
     4561 cggacagatt ggatattggt tcgactcagg cgttgcgcca atactttcag gttgtcttga
     4621 gcttcggcgc gcaatgaatc cttatcgaaa ccgaacaggg ttttggcaga cagggaaatg
     4681 gtttcatcaa catattgcgg agcctgctcc acaacgacga caggcgcggg tgcgggttcg
     4741 ggttctggct cgggttcggg ggcagcaacc gcatcgccgc attctacgcg accttggctt
     4801 gctttatcaa agtaggcgtt tttccagcat tcgccatagt tgttgcgtac gatttcgttc
     4861 gactggccgc ttacggtgta accctgaacg gacgcctcgc ccgcaacagc agtgccggaa
     4921 gcgagcaatg caacgaataa tgcgcttaat ttcagctgtt tggtcatttt attccctcat
     4981 taaatttgta cagcagatac agaagcaact gctgaattgc caacatacag gccgctgtag
     5041 aaacatggcg gcaaatcgga tatttcagtc tccactataa agaagcaggg ggcggactgt
     5101 caatactcaa cgcctgaaaa caaccgtaaa acagcctgtt tcctgatagt ctatcatgcc
     5161 gaaaaatcaa ttccgtgtca ttactttagg gggatttttt ccatatcgca cgattgccgt
     5221 tgcacaaaaa caacaaacac aacggcaaag ccccataccg taccggcaag aaaatatgat
     5281 aaattataaa caatgttcag ccacccgaca cagacccaca ccgacccgcc atgaaattac
     5341 aacaattgaa atacgcctta gaagtttacc agcacaacct gaacgtttcc gaagcggccg
     5401 aagccttgtt tacttcgcaa cccggcatct ccaaacaaat caaattgctg gaagaagaaa
     5461 tcggcattca gatttttatc cgcagcggca agcgcgtggt ttcggtctcg cagccgggca
     5521 aggtggtttt ggatattgcg gaacgtattt tacgcgatgt acagaacatt aaaaatatcg
     5581 gcagcgagtt taccggacag gacagcggtt cgctgacggt tgccacgacg catactcagg
     5641 cacgctatgc cctacccttg attgttgccg attttgtgaa acgctatccg aaagtcaacc
     5701 tgaccatcaa acaggggagc cctgccgcca tcgcacgtat ggttacctca ggcgaggcgg
     5761 acttggcgat tgttacggaa cggatagacg accatcccga actgggaaaa cttccctgct
     5821 atgactggac tcatgcggtt atcgtaccga acgaccaccc cttgctcgaa tgcagaaacc
     5881 ccctccgtat tgaagatttg gcgaggtttc cgctgattac ttatgaattt gcattcaatg
     5941 cgggcagcag catcgcgcgg gcattttcca aagcccgttt ggaacagccg gatgtcgcat
     6001 tggcggcggc ggatacggac gtattgaaga cttatgtgcg cttgggtttg ggcgtgggac
     6061 tgatggcgaa aatggcgtac aacccggata cggacggcga tttgcagctt gtggatgcgg
     6121 cacacctgtt cgagccgtcg ccgacgtgga ttgctttgcg cagcgatact tatttgcgcg
     6181 gatatgccta cgactttatc caagcgtttg cgccgcacct gacacgcgag aaggtggata
     6241 ggattctgta cacgcccatc agcgaggatt tttcgattta ggcggctgcc ggttttcaga
     6301 cggcattttg cggcagataa aacaaacagg gcagatgttt tcttctgccc tgtgtttatt
     6361 gagaatgccg tctgaaatgt tcgtacaggt taatcaaatg gcgtgcgagc agccggacac
     6421 catttttttc aacacctgca gattgaggat cttgatgtgt ttgtgttcga cggaaatcaa
     6481 tccttcctga tgaaatttag ataatgtgcg gctgacggtt tcaagtttca gcccgagata
     6541 actgccgatt tcttcgcggg acattcttaa gatgaagtcg ttggcagcaa aacctcggga
     6601 ataaaggcgt tgggaaaggt tcagcaggaa ggcggcaatc cgctcttcgg cgcgcatatt
     6661 gcccaacagc agcataacac cttggtcgcg cacgatttca cggctcatca tgcggaagaa
     6721 gtgcgtacgc aggctgggga tgttttgccc cagttcttcg atgtgggtaa acggcagttc
     6781 gcacacttcg ctgtcttcca aggcgaccgc gtcgcaactg tgcacatggg aacagatgcc
     6841 gtccatgccg atgagttcgc ccgacataaa gaaacccgtt acctgatcgc ggccgtcctg
     6901 actggcgacg gttgttttga agaagcccga acggatggca aagagcgagg taaaggcttc
     6961 gccgacacag aacaggtatt cgcccttttt caggcggcgg ctttgacgga tgacggcatc
     7021 gagttggctg agctcgttgg gcagcagccc gacaggcagg cagagttccc gcaaagaaca
     7081 ggaagaacac agcgttttca tctgatgtgt agtattatgc gaagccatac cgtacctttt
     7141 tgtgcgcttt gccccatcat gattatagtg gattaaattt aaaccagtac ggcgttgcct
     7201 cgccttgtcc ttatttaaat ttaatccact atatgtgctt attgacacat atcaagacag
     7261 gtttatcata ctgtggcatt ctaccaaact atccaaaatt tgactaacat cataaaaccg
     7321 cacacacaca ccatgaaaat cattcagata cagaacaatc acaatgtaaa cgatgaccgc
     7381 cccgagtttg accgcgcgct gattgccagc ctgcccgcca gcggcccgcg ctacacttcc
     7441 taccctaccg ccgaccgttt ccatgacggt ttccgcgaag gcgaatatat caaagcttta
     7501 catttgcgcg gtatgggcgc gttaaacaaa ccgctttccc tttacattca cattccgttc
     7561 tgcaacacca tctgctacta ctgcggctgc aacaaaatca tcaccaaaga caaaagccgc
     7621 gccgatgcct acatcgaata tcttgaaaaa gaaatggaac tgctcgctcc acatctgaac
     7681 ggacggcacc agcttgccca actgcacttc ggcggcggca cgccgacctt tttgagcgac
     7741 gaacagatcg aacgtgtctt ccgcatgata cgcaaacatt tcgagttaat ccccaccggc
     7801 gaatactcca tcgaaatcga cccgcgcaaa gtcagccgcg acaccgtcct catgctcggc
     7861 agactcggct tcaaccgcat gagcatcggc attcaggatt tcgaccccaa agtgcaggcg
     7921 gcggtcaacc gcatccaaag ttacgaagaa accaaagaag tcatcgatgc ggcgcgcgaa
     7981 gcggggttca aatccgtcag cgtcgatttg atttacggcc tgccgcacca gacttcggaa
     8041 agcatcaaaa ccaccatcga taccgttttg tcgctcgatc ccgaccgcct cgccctttat
     8101 cactacgccc acctgccgca cgtgttcaaa ccgcaacgcc gcatcgatac cgccgccgtt
     8161 cccgacagcg aagagaagct cgatatgctg caatactgcg tccaaaccct aaccgaacgc
     8221 ggctacgtct tcatcggcat ggatcatttc gccaaacctg acgacgaact ctccatcgcc
     8281 ctcaaagaag gcttcctcca gcgcaacttc caaggctatt cgacctacgc ggattgcgat
     8341 ttggtcgcca tcggcgtgtc gtccatcggc aaaatcggca gcacctattc ccaaaacgaa
     8401 cgcgacatcg atgcctacta tgccgccatc gacgaaggca gactgcccat catgcgcggt
     8461 tatcagctca atcaggacga catcctgcgc cgcaacatca ttcaggattt gatgtgccgt
     8521 ttcgcgctcg actaccggat ttacgaaagt atgttcggca tcccgttcga ccgctacttc
     8581 aaagacgaac tggcggattt ggaaaaactc gccggtttgg gattggtgcg cctgaacagc
     8641 cacgggctga ccgttacccc gaaaggacgc ttcctcatcc gcaacatcgc catggtcttc
     8701 gattaccacc tgcgccataa agaaaccaag gcgaaatatt cgcaaacggt gtgattgcgt
     8761 ctaacgtaca aatgccgtct gaaaggcttt ttcagacggc attttgctgc cggcaggata
     8821 agtgttttca agaacaggcg gcggcatatc ataacgttcc gcacctttgt gtccgaccgt
     8881 tccgaaacca agatatagtg gattaacaaa aaccagtaca gcgttgcctc gccttgccgt
     8941 actagctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat cactatacca
     9001 aacggcatat cccgacagaa cagattgtgc ataaggcaca agcccgcaca ttccatcaac
     9061 aaaatgccgt ctgaacacgg gttcagacgg catcagtatt ttacaatcag aatactgcct
     9121 gtaaaaccaa gtagcagaca accgacaata cggcggcggc aggcagggta atgacccacg
     9181 ccaaaccgat gggcttcatc agtttccagt tggcattgcg gttgaccaga ccgatgccga
     9241 gtaccgcgcc gaccaagata tgcgtactgg acacgggcag ccccatcagc gacgcgccca
     9301 tcacgacgga ggcggcggac agttcggcgg taaaacccga agcaggatgc atttccgcca
     9361 aactcgtacc gacggtttta atcacctctt taccgacaaa ccacaaaccg acaatcagcg
     9421 cgataccgaa agtcagcatc gcaatcgggg ggacgacatt ttgcgcggca acgctgttgg
     9481 tacgcaaaac atccataatc gcggcaaacg gaccgatggc gttggcgata tcgttcgcac
     9541 cgtggctgaa tgcgaagccg caggcggtaa agacctgcat ccatgaaaac atctgaaagg
     9601 tcgatttgcc caagtcttta cgcttgaggc ttttggcaaa cacaaacgtc cccatccaca
     9661 ccgccgcgcc tatcataaag atggtcagga agctgttgac gttgctcatc cccaaatgca
     9721 ggtttttcaa gcccttgaaa atcagcatag cggaaatcat catcgcgccg aacgaagcga
     9781 taaagggaat ccaagaatgc agcgctttgt aggaatcgac attgttttta cggttgtcga
     9841 acgcataaag accgcggtaa tactccgatt gcagctcttg cggatcgaat tcgggttcgt
     9901 cgtaaatttg cgcgtcgtgc gccattttgg tggcgtactc gactttttcg gcttcggaca
     9961 aaccctcgaa aaacaggcgg tgccgttctt tataggcctt tttttcctgc ttgatgccct
    10021 tgagcgtgcc ttccgcccaa gcgttgtaat ctaagacgtt tttcttgacg cgcgaaaaca
    10081 gaaaatagga caccgcgccg cccaacacgg gcgacaatac ccaagaaaca ccaataccgc
    10141 ccagcttgcc ccaacgtatc aaatcgcccg atgcggcatc gttcattacc gccatacata
    10201 ccgcgctgcc gacaatgccg ccgataatgg aatgggtggt agataccgga agcccttttt
    10261 tcgaggcaaa caacagccac aacgccgccg ccaaaagcgc ggacatcata ataaacacaa
    10321 actgtatggg ttcgaaatca acacccttca aatcgacgat gcctttgcgt atggtattgg
    10381 ttacctcgcc gcccgcgatg accgcgccgc tgacctcaaa taccgccgca atcagcaaag
    10441 cctgcgggat ggtcagcgta cccgcaccga cactggtgcc gaaagaattg gcaacatcgt
    10501 tgccgccgat gttgaacgcc ataaacacgc cgaacatcgt ggcaataatg aataaggcgg
    10561 cgtaattgta atgggtataa cccaagcccc aataaataaa atagccgact gtacctacca
    10621 gcatggcggc aaagacggcg ttgatggttt taacatttgc gctcatttgg atttgagcca
    10681 tggcaatctt cctttaatgt ggtggaaatc atgagtgttg ccgacactgt actgctgcct
    10741 ccggtcgaac tgccgtttcg ggatatggca gacgcatcct tccttctgaa ccgcatccga
    10801 atacccccaa acctgaccga atgcaaaact gctgccggaa acggtttgta tgacacttct
    10861 actactggat gttgcgggcg cattataata tttttccatc cgtcttgaaa catttattta
    10921 cactttattt acactgcggc ggcaaatcgg tatacgagcg tcaatacacg ttaaaatggc
    10981 gttttgcacc agtttgggag tgatgatgga aacacagctt tacatcggca tcatgtcggg
    11041 aaccagtatg gatggggcgg atgccgtgct ggtacggatg gacggcggca aatggctggg
    11101 cgcggaaggg cacgccttta ccccctatcc cgacaggctg cgccgccaat tgctggattt
    11161 gcaggacacg ggggcggacg aactgcaccg cagcaggatt ttgtcgcaag aactcagccg
    11221 cctgtacgcg caaaccgccg ccgaactgct gtgcagtcaa aacctcgcgc cgtccgacat
    11281 taccgccctc ggctgccacg ggcaaaccgt ccgacacgcg ccggaacacg gttacagcat
    11341 acagcttgcc gatttgccgc tgctggcgga actgactcag atttttaccg tcggcgactt
    11401 ccgcagccgc gaccttgcgg ccggcggaca aggtgcgccg ctcgtccccg cctttcacaa
    11461 agccctgttc cgcgatgaca aagaaacacg cgtggtactg aacatcggcg ggattgccaa
    11521 catcagcgta ctcccctccg acgcacccgc cttcggcttc gacacagggc cgggcaatat
    11581 gctgatggac gcgtggacgc aggcacactg gcagcttcct tacgacaaaa acggtgcaaa
    11641 ggcggcacaa ggcaacatat tgccgcaact gctcgacagg ctgctcgccc acccgtattt
    11701 cgcacaaccc caccctaaaa gcacggggcg cgaactgttt gccctaaatt ggctcgaaac
    11761 ctaccttgac ggcggtgaaa accgatacga cgtattgcgg acgctttccc gttttaccgc
    11821 gcaaaccgtt tgcgacgccg tctcacacgc agcggcagat gcccgtcaaa tgtacatttg
    11881 cggcggcggc atccgcaatc ctgttttaat ggcggatttg gcggaatatt tcggcacacg
    11941 cgtttcctta cgcagcaccg ccgaactgaa gctcgatccg caatgggtgg aagcagccgc
    12001 atttgcgtgg ttggcggcgt gttggattaa tcgcattccc ggtagttcgc acaaagcaac
    12061 cggcgcatcc aaaccgtgta ttctgggcgc gggatattat tattgagcac aaataccgtc
    12121 tgaaagccat ttccgccgct cagacggcat tttcgcccct tttgtttaca aacccttaaa
    12181 atccatttac actcaaactc gttcaaaaca aaacaaaccc tgccatgaaa accctgctcc
    12241 tcctcatccc cctcgtcctc accgcctgcg gcacactgac cggcataccc gcccacggcg
    12301 gcggcaaacg ctttgccgtc gaacaggaac tcgtcgccgc atcgtcccgc gccgccgtca
    12361 aagaaatgga
//
LOCUS       DAWGJV010000050        12347 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWGJV010000050 DAWGJV010000000
VERSION     DAWGJV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.050"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..342
                     /locus_tag="WHQ94_001005"
     CDS             <1..342
                     /locus_tag="WHQ94_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2128301.1"
                     /translation="LLEGLPKGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRP
                     LSEVQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCLNLLK
                     AANRLSAPAAA"
     gene            560..1339
                     /locus_tag="WHQ94_001006"
     CDS             560..1339
                     /locus_tag="WHQ94_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ2128302.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALAEHPLVGEYGHSFRFVPVTTRAANPSGLGGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1550..2962)
                     /locus_tag="WHQ94_001007"
     CDS             complement(1550..2962)
                     /locus_tag="WHQ94_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ2128303.1"
                     /translation="MLFEFKENKMIVTSRWPLLEKAFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDSMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            3053..3129
                     /locus_tag="WHQ94_001008"
     tRNA            3053..3129
                     /locus_tag="WHQ94_001008"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3087..3089,aa:Arg,seq:acg)
     gene            3147..3221
                     /locus_tag="WHQ94_001009"
     tRNA            3147..3221
                     /locus_tag="WHQ94_001009"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3180..3182,aa:Glu,seq:ttc)
     gene            complement(3397..3660)
                     /locus_tag="WHQ94_001010"
     CDS             complement(3397..3660)
                     /locus_tag="WHQ94_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128304.1"
                     /translation="MNLKDFLYIRRVLEEYMRYLHNPDHYPDIEAIQNFLGDASSGGG
                     FECLSTAIYNKVYKVDLPAKIEKMIDDGLFEHPLYPSYYKKNE"
     gene            4263..5048
                     /locus_tag="WHQ94_001011"
     CDS             4263..5048
                     /locus_tag="WHQ94_001011"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ2128305.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            5085..5957
                     /locus_tag="WHQ94_001012"
     CDS             5085..5957
                     /locus_tag="WHQ94_001012"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ2128306.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKQRISG
                     LITLLCRQDKVSAA"
     gene            complement(6017..6628)
                     /locus_tag="WHQ94_001013"
     CDS             complement(6017..6628)
                     /locus_tag="WHQ94_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ2128307.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            6737..6961
                     /locus_tag="WHQ94_001014"
     CDS             6737..6961
                     /locus_tag="WHQ94_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ2128308.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            6962..7567
                     /locus_tag="WHQ94_001015"
     CDS             6962..7567
                     /locus_tag="WHQ94_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128309.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSCVRHQDFARVAVDLF
                     RSACVLTQGSFEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYL
                     SLPRDCCSVKAERADSAVLLEHFDGLVRQPPKNTNIELRAEQSVQALFETRQISYAWR
                     ESGSPEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            7627..8661
                     /gene="pyrC"
                     /locus_tag="WHQ94_001016"
     CDS             7627..8661
                     /gene="pyrC"
                     /locus_tag="WHQ94_001016"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ2128310.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVVPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATSELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFTSKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(9028..9453)
                     /gene="nusB"
                     /locus_tag="WHQ94_001017"
     CDS             complement(9028..9453)
                     /gene="nusB"
                     /locus_tag="WHQ94_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ2128311.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9531..10007)
                     /locus_tag="WHQ94_001018"
     CDS             complement(9531..10007)
                     /locus_tag="WHQ94_001018"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ2128312.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPITLMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(10062..10385)
                     /locus_tag="WHQ94_001019"
     CDS             complement(10062..10385)
                     /locus_tag="WHQ94_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ2128313.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQSVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQAVAD
                     WQPQD"
     gene            10552..11271
                     /gene="rnc"
                     /locus_tag="WHQ94_001020"
     CDS             10552..11271
                     /gene="rnc"
                     /locus_tag="WHQ94_001020"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ2128314.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYTFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     SAAKEALQWLEEKLPLKKKKK"
     gene            11318..12241
                     /gene="era"
                     /locus_tag="WHQ94_001021"
     CDS             11318..12241
                     /gene="era"
                     /locus_tag="WHQ94_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ2128315.1"
                     /translation="MDIETFLAEERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDQRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         2933 a         3101 c         3278 g         3035 t
ORIGIN      
        1 ttgctggaag gactgcccaa aggtacgacc gtctatgccg acaaaggcta cgacagtgcg
       61 gaaaaccggc aacatctgga agaacatcag ttgttagacg gcattatgcg caaagcctgc
      121 cgcaaccggc cgctgtcgga agtgcaaacc aaacgtaacc gatatttgtc gaagacccgt
      181 tatgtggtcg aacagagctt cggtacgctg caccgtaaat tccgctatgc gcgggcagcc
      241 tatttcgggc tgctcaaagt gagtgcgcaa agccacctga aggcgatgtg tttgaacctg
      301 ttgaaagccg ccaacaggct aagtgcgccc gctgccgcct aaaaggcggt ccggatgcct
      361 gattatcggg tatccgggga ggattaaggg gatatttggg taaaattagg aggtatttgg
      421 ggctaaaaca gccgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
      481 caaaggtctc agaccgtcaa tgcggaaaaa aacacccaaa ttggctataa tcccgacaaa
      541 cacactcaag gacaacaaca tggcagcctc gcccgaagca aaattcactg aagaaaagat
      601 tttgtgggtc aaacaccaca cgccgaaact catcactttc gccatcagcc gtcccgaatc
      661 ctaccgtttt aaagccggac agttctcccg actcggtttc tacgaagggg aaggctttat
      721 ttggcgtgcc tactccatcg tttccgcaga atatgccgac acgctcgaat attttgccgt
      781 actcatccaa gacggcccca tgtcggcccg tttcgccaaa atgcaacagg gcgataccat
      841 cctgctcgat aaaaatgcca ccggcttcct cctgcccgaa cgcttccccg acggcaagga
      901 tttggtgatg ctctgcaccg gctccggcat cgcccccttc ctttccattc tcgaacaacc
      961 cgaaatccgg cagcgtttcg ataccgtcaa cctgatacat tccgtatctt ttcccgaaga
     1021 attgattttc aacgaccggc tcgccgcatt ggccgaacat cccctggtag gcgaatacgg
     1081 acactcgttc cgtttcgtcc ctgttaccac ccgtgccgcc aacccctcgg gcttaggcgg
     1141 aaaacgcatt ccggaactct taaaaaacaa cagcatcgaa caggcgctgc ataccaagtt
     1201 tacccccgaa tccacacggt ttatgatttg cggcaatccg gaaatggtca aagacacctt
     1261 ccaaacgctg cttgacatgg gttacgccat gcaccgcaac cgcattcctg gtcaaatcat
     1321 gatggaaaac ggcttctaaa aaccaccctg cttgtccgat gccttcggtt ggaagagcaa
     1381 accgacacgg cacgaaaacc gcgtcggcaa aaatgccgtc tgaaaaaatt cagacggcat
     1441 cttcggacac attacctgca aacggcaaca cattccaacc caaaccgatt aggcaatcaa
     1501 tacgaacggc tgtttacata cttaccggct ttcatcaacc ggtatcgatt taaccgattt
     1561 ccttaatatt tttcctgtcc gttttaaact tcgccttaaa cgcatccggt aaatctttat
     1621 cgaaatacca aagcccgtca cccatttcca atgcgccgcc cattccgtgc agaacaactt
     1681 tttcccccgt taaaggatca gtttcggtaa tttctacctc atgcattttt ccccaatcca
     1741 aaataggcaa cttgtgtgca aacgccaaaa cctgttcgtc agaacggccg cacgccttat
     1801 cgcatcggcc tgaaacatat acgggacaag gctcatcctc tgcataaccg tccggaagga
     1861 taccggctga ggcaggacac aatccgtatg tttccgccca cgacgacaaa gcccgtctcg
     1921 ccgccttttt attggcaaat aatccggtag gcggattatc cgtcacaccg tttttcaaag
     1981 ccgctgtttt cgcattcaac atgccgtctg aacctttttc aaacctgacg gtcgtaaatg
     2041 ttttaagcag atttttggca gatacataac aacccgaatg ataacgtccg accaattccg
     2101 ctttaatctt atatgcatgc aggctgccca atgcaggaaa aaaacggact tcctccgtat
     2161 tgcaccaatc aaacggggct tttccggaat ccaataaagc cgaaacctcg ctatataccc
     2221 gttcaaacgt accgatgtaa tttactttcc ctcctccgtc gaaacaagcc agcaccccca
     2281 tactgtcagg caaaccgtac aactgttccc tcaaccgttc gggcagcgcg gcaggcagcg
     2341 gtttcggatt catcaaacgg aaacactgcc tgatccatgc ctcgaccccg tgttccgaca
     2401 gactgtattc caaataatcg cacaatgccg atacatccgc catcgcacga tgcctgtctt
     2461 ccacaacaat ccccaacctt tcgatgatac tgtccaggct gtgcttgtaa aattgcggat
     2521 acagacaccg ggacagttgc acactgcaca aagcaggcga tgaaaatccg atacccgcac
     2581 gatgaaactc atgctttaaa aacgtatagt cgaaacggct gttatgtgca accagcacac
     2641 aacccttcaa taccgaaaac aactcgccgg caatctctgc aaaaacaggt gcgtcggcaa
     2701 ccatgccgtc tgaaatcccc gtcagccccg caacaaactg cggaatcggt ttttgaggat
     2761 taaccaacca ctcatgcctc accacccttc cctgctcaaa cttgaccaaa gccacttcgg
     2821 ttaccctgtc ttcatacaga ttgccgcccg tcgattccaa atcaaccacg gcaacaggca
     2881 ttccaaaccg taaaaatgcc ttttccagca agggccaacg ggaagtaaca atcattttat
     2941 tctctttaaa ttcaaacaac aaaccgatat tttacacttt tgaggcattt catccaacaa
     3001 aacaattgac agaatccgat gattacccta aaattcaaat ctttcttgca gcgcacccgt
     3061 agctcagttg gatagagtat ctggctacga accagagggt cgggcgttcg aatcgctccg
     3121 ggtgcgccag taagaaaata caatatgcgc ccatcgtcta gcggttagga catcgccctt
     3181 tcacggcggt aaccggggtt cgattccccg tgggcgtgcc aaattccaaa tcccccgaaa
     3241 taatccctcg gggatttttt attgcctcgg caactcgtta ccatagttaa aatttaacaa
     3301 gcactatttt cactttttat cctttcctac aacttcgaat ttacgtatcc acataaattt
     3361 atattttcta caatttcaac actatccaat tattgcttat tcatttttct tatagtaact
     3421 gggatataga gggtgttcaa ataaaccatc atcaatcatt ttctcaatct tcgccggcaa
     3481 atcaacttta tatactttgt tataaatagc tgtgcttaaa cattcaaatc cgcctcccga
     3541 agaagcatca cctaaaaaat tttgaattgc ttctatatcg ggataatgat caggattatg
     3601 tagataacgc atatattcct ctaaaactcg tctaatgtat agaaagtctt tcaggttcat
     3661 tgttattttt tccttatcag aaatagcatc taatccatat tcaacgatat cttttatact
     3721 tgtatatttt atcatcatct ttacctaccc ccttcatcag aatctcagac gtaatcgaat
     3781 catattcaaa cctttgccgt gcaaaccgat atcacataac cggatgcggt gtccgttcaa
     3841 cattttaccc gattgaaacg cctgatatat cgcaccccat caaaggggca ttgcttttct
     3901 taacaatccc ctttgacagg gcttttttat gccatcatca aatttatagt ggattaactt
     3961 taaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgga
     4021 gcaccaagtg aatcggctcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     4081 atttttgtta atccactata atccgagatg cttgccgttt atttccgcct cgttcaaacg
     4141 gcggctctga tttgcgcggt ttctgtttgc cgtattcgcc tatccgtacc gcaaatgtta
     4201 tactgggaaa aatttactga ttgtgtttta cggcatattt gccgatagga tggaagagac
     4261 aaatgagcag aatccggcag gcttttgccg ctttggatgg cggaaaggca ttgattccct
     4321 atattacggt gggcgacccc gatattcgga caactttggc attgatgcac ggcatggttg
     4381 caaacggtgc ggatattttg gagttgggcg tgccgttttc cgatccgatg gcggatggac
     4441 cggttattca gcgtgcggcg gagcgggcgt tggcgaacgg gatttcgctg cgcgatgtct
     4501 tggatgtcgt cagaaaattc cgtgaaaccg acacgcaaac tccggttgtt ttgatgggat
     4561 atttgaaccc tgtacataag atgggttatc gggagtttgc tcaggaagcc gcaatggcgg
     4621 gtgtggacgg cgtgttgacg gtggattccc ctgtcgaaac catcgatccg ctctatcgcg
     4681 agctgaagga taacggggtc gactgtattt tcctgattgc gccgacgacg acggaagacc
     4741 gtattaaaac cattgccgag ctggcaggcg gatttgtcta ttatgtttcg ctcaagggcg
     4801 taacgggcgc ggcaagtttg gatacggatg aggtttcgcg taaaatagag tatttgcatc
     4861 agtatatcga tattcccatc ggtgtcggtt tcggcatcag caatgcggaa agtgcgcgca
     4921 aaatcggccg ggttgccgac gcggtcattg tcggcagccg gattgtgaaa gagattgaaa
     4981 acaatgcggg caacgaggct gcggccgtcg gtgctttggt caaagagttg aaggatgccg
     5041 tgcgctgatt ctgtcacgca ttctaaatat tttaggagtt gtccatgagc tggttagata
     5101 aaatcctgcc acccaaaatc aaaaaccgca gcaaagacgg ttcttccaat gttcccgagg
     5161 gtttgtggca caaatgtccg tcttgttcgg caaccgttta ttcgaccgag ttgcagcaga
     5221 acaatcaggt ttgtccgaaa tgcaaccacc acaatccgtt gtcggcacga caacgcctga
     5281 atctgctttt ggatgaggat ggcagagagg aggttgccgg aaatgtcaaa ccgacagatc
     5341 ctttgaagtt taaagacggc aaaaaatatc cggatcgttt gagtgcggca cgcaagctga
     5401 ccggggaaga tgatgctttg gtggtaatga aaggcaagat gaacggtctg cccgttgtgg
     5461 ttgctgcgtt tgagttccgc tttatcggcg gttcgatggg ttcggttgtg ggcgaacgat
     5521 tcgtacaagg tatccgtcgg gcggttgccg acaattgtcc gttcgtctgt gtggcggctt
     5581 ccggcggtgc gcgtatgcag gagggtgtga actcgttgat gcagatgacg aaaaccagtg
     5641 ccgcgctgca tttgctgacg gaaaaacgcc tgccgtttat atcggtattg accgatccga
     5701 ctatgggcgg cgtatccgcc agcttcgcat ttttgggcga tgtcgtgctt gccgaaccca
     5761 atgcattaat cggctttgcc ggtccgcgcg tgattgagca gacggtgcgc gaaacgctgc
     5821 cggaaggctt ccagcgtgcc gagtttttgc tggaaaaagg cgcaatcgac cagattgtcg
     5881 accggcgcga gatgaagcag agaatcagcg gtttgattac gctgttgtgc cgtcaggaca
     5941 aagtttctgc cgcctgatgg ctgatgaatc gaataccgtc tgaaaccgat gtttcagacg
     6001 gtatttttgt gtctggttat ttgtcgtgcg gcttcatcga cggggcatag cgtccggcac
     6061 gttctttcag gcgttgtacc aaacctttcg tgtcggcggg tacaccgccc tcgcagaatg
     6121 cctgatacag gacggtgcgc agtgcgtcgt tgcggcttaa tgtaccgcct atcggtttcc
     6181 attcggcgtt tcggggctgt atccagcggc ggttgaccgt gtcgccgtag ccgaacactt
     6241 tataggagga aggtttgccg ttgccgaacc tgatggagaa gcgggcgcag aatatgcctt
     6301 cggcagtcag gttgtcgtag cctttgtcgg agcggatatt gagaatgtag cggatgctgc
     6361 cgtcgggcgc gggcataatt tgcaggctgt cgagcaggat tttcggctgt ttgccgtaat
     6421 tttcatccac ataaatgtca aaccagccgt ccgagtgcgt atcgggcagc ggcggcagtt
     6481 tggcggtatg ttctttaaat tcgcgggcgg cggcttcttc gggcgtttcg cggtagcggg
     6541 tgttgatcgg cgtgtctttt tggttgaagc cggcagcaag ggacgtgccg gcggtgagtg
     6601 cgagcagcag gagggcggtg cggcgcataa gtttctccaa attgaaaacg gcgttatttt
     6661 atgggttggc aaagggggct gcaagcaact ggggtataat ctccccccga ttcccatttt
     6721 ttaaacggta caaacgatga acagcgaaac tttagacgta accggattga aatgtcccct
     6781 gccgattttg cgggcgaaaa aggctttggc gcaaatgcag cagggcgacg tgttgaccgt
     6841 tctggcaacc gacggcggcg caccggggga ttttgaggct ttttgccgcc aaaccggtca
     6901 tgtgctgttg gatgcttccg aacaggacgg cgtgttcacg ctggtcgtca agcacaaata
     6961 aatgccgtct gaaatgcgga tgtcccgatt gatgttattg ctgtcggcat ttgtgcttgc
     7021 tgcgtgtaat gcccaaaacc ggcaatcctg tgtacggcat caggatttcg cacgtgtagc
     7081 cgtcgatttg tttcggagtg cctgcgtcct gactcaaggc agtttcgaac cagtttccaa
     7141 gtttgccgct gtcggacatt ttgtccctgt cggaaaggaa gacctgtccc gtctgccgcc
     7201 cgctgttgca gaacccgatg tacaggcgtt gtggacgctt gaacgcgatg gcggcattta
     7261 ttatttgagc ctgccgcgcg actgttgcag tgtgaaagcc gagcgggcag acagtgccgt
     7321 gcttttggaa cattttgacg gacttgtccg acagccgccg aaaaatacca atatagaatt
     7381 gagggctgaa cagtcggtgc aggcactgtt tgaaaccagg caaatcagct atgcctggcg
     7441 ggaatcgggc agccccgaag aaaccgtatt gaccgcacaa accgccgatt ccccggattt
     7501 gcccgtacag gcagttttga acctgacgca ccgttcccac aacgggaaac ccctaattct
     7561 cccctaactt cagacggcat aaagtcggca tgccgtctga acattcaaat aaaacaaagg
     7621 aaaaccatgc aaaccctgac cattatccgc cccgacgata tgcacctgca cctgcgcgac
     7681 ggcgacgcgc tcaaagccgt tgtgccttat accgccagac agatggggcg cgccgtgatt
     7741 atgcccaatc tcaaaccgcc tgtcgtcagc gttgccgacg cgcttgccta caaagcgcgc
     7801 attatggcgg cgttgcccga aggcagcgcg tttgagccgt tgatgacgct ttatttgact
     7861 gataacgcca cgtccgaact tgtacgcgaa gccaaagctg ccggcatcgt tgccttcaaa
     7921 ctctaccccg ccggcgcgac caccaattcc gattccggcg taaccgacct gttcaaactt
     7981 atccccgttt tggaagaaat ggcgaaacag ggcatcctgt tccttgttca cggcgaagta
     8041 actgaccccg aaatcgacat cttcgaccgt gaagccgcct ttatcgggcg cgtgatgaaa
     8101 cccgttttgg cgcaagtacc aaatcttaaa gtcgtgttcg aacacatcac caccgccgaa
     8161 gccgcccgcc tcgttttgga agcaggcgac aacgttgccg ccactgttac cccgcaacac
     8221 ctcttgctca accgcaacga cctcttggtc ggcggcgtgc gcccccatca tttctgcctg
     8281 cccgtactca aacgcgaaac ccaccgtcag gcattggttg ccgccgttac cggcgagaag
     8341 gcgcacaaat tcttcctcgg cacagattcc gcgccgcacg ccaaatccgc taaagaaaac
     8401 gcctgcggtt gcgccggtat gttcagcgcg atgaccgcca tcgagcttta cgccgaagtg
     8461 tttgaaaaag caggcgcgtt ggacaaactc gaagccttta cctcgaaaaa cggcgcaagg
     8521 ttttacggca ttccggaaaa cgccggcacg atcaccctcg tcaaacaaag ccaaaccgtc
     8581 cccgcaagtg ttccctacgg cgacggcgaa cttgtcccga tgcgcgcggg cggcgaaatc
     8641 ggctggacgg tacagtattg attgaacggt caagcaaaat gccgtctgaa aggtttttca
     8701 gacggcattt gtgttgttga ctgattgata tgtcaacggc gtttgagttt gttacgtttc
     8761 ggttatttcc gataaattcc cacaattttc aaatttcgcc attcccacga aggcaggaat
     8821 ccggaaattc gatgcgacca gagtttatca aaaacggcag caactcaaaa aaccggattc
     8881 ccgcctgcgc gggaatgacg agattgaagt ttcagaattt atttgaaata cccaaaattc
     8941 aaaaaaccaa attcccacct gcgtgggaat gacgaaacaa agaaagcaga aataaggaca
     9001 tagaactttc tttaaatttg tgatgcatca acggcgtttg ggctcgtcgg ggcggatttg
     9061 ggcggcgagt ttgtcgagga tgccgttgac gaatttgtgc ccgtccgtac cgccgaaggt
     9121 tttggtgact tcgatggctt cgttgatgat gacggggtag ggcgtttcgg gcatggcgga
     9181 caactcgtgg caggcggtca gcaggacggc gcgttcgatg gggttgaggt ctttttcgtc
     9241 cctgtcgagc agcgggcgga tttgccggat gtattctgcc gcattggttt gcgtgccgaa
     9301 gaaaagtttg ttgaacaatt cttcgtccgc tttggcaaag tcggacattt cgcggatgtt
     9361 tttagcaatc tcgggtgctg cggtgcggtt gataagggat tggtaaacgg cttgtacggc
     9421 aagctcgcgg gaacggcggc gggctgtttt cataattttt ccttgaaacg gttgggcggc
     9481 acggtatgcc gtctgaaacg gaaagggcgc atcggtgtac gccctgtttg ttattcttcg
     9541 tcttcaaact gttcttcgag cagaaggttg acgaggttgg cgcattctac ggcgactttg
     9601 gcggcatccg aggctttttc ttcaatccgt tcgattgcct gtgcgtcgtt ttcggtggtc
     9661 aggacggcat tggcaatcgg gatgttgtag tcgagtgcga cgcggctgac ccctgctccg
     9721 gattcgttgg aaaccagctc gaaatggtag gtttcgccac ggataacgac gccgatggca
     9781 atcagtgcgt caaatttttc agaagaggca aggttcatca gcgtgatggg gatttcaagt
     9841 gcgccgggta cggtggcgac ggtaatgttt tcgtctgcca cgcccaattc ttggagggtg
     9901 cggcagcaga ctttgagcat ttcgctgccg atttcgttgg tgaaacgtgc ctgtacgatg
     9961 ccgatgcgga ggtgtttgcc gtcgaggttg ggggcgatgg tgttcatctg gattcctttg
    10021 tggatcggta atttggatgg ttgggatgcc gtctgaaggt ttcagtcttg cggctgccag
    10081 tcggcaacgg cttggaatgt gccgtcttcg gcaagctccc acgcgctgcc ttcgggttgg
    10141 gaaagcagtg cggcggtttc agggttggtt ttggcgatgt cggcgaggct gacgatgctg
    10201 aagttgtccg gatcgtcggt gtattcgtcg gtctcgtcgc cgctgaagaa acgccagccg
    10261 ctgtcatttt cgaaaacggg ggcttcgcgg tagaggaagc cgacagactg gttttgtttg
    10321 gcgacggtgt tggtggcgat acagcggtcg agtgccgagg aaagtgcttg tgcaaatgcg
    10381 ttcattacgg gaatacgttg ggggaaaact tacggatttt accacgattc gtgcgttgtc
    10441 ggcagacggc ggcggtttgg tggtacaatg tgcgccgttt gcagccttaa ggtgtttctg
    10501 tatttttgga gtatggaaac gcattcgggc tgttttttgc ggaagacggt aatgaaagac
    10561 gatgttttga aacagcaggc acacgcggcg atacagaaga aactgggcta cacgttccgc
    10621 gatatttcgc ttttgcggca ggctttgacg cacaggagcc atcatgcgaa gcacaacgag
    10681 cggttcgagt ttgtcggtga ttcgattttg aattatacgg tggcgcggat gctgtttgac
    10741 gcgtttccga agttgaccga gggcgagttg tcgcggttgc gggcaagtct ggtcaatgag
    10801 ggcgtgctgg cggaaatggc ggcggaaatg aatgtcggcg acggtctgta cttgggggcg
    10861 ggcgagttga agagcggcgg cttcagacgg ccttcgatac tggcggacgc gatggaggcg
    10921 atgtttgccg ccgtcagctt cgatgccgat ttcaacacgg cggaaaaggt ggtgcgccat
    10981 ttgtttgccg atcgcgtccg gcgtgccgat ttccaaaatc aggcaaaaga cggcaaaact
    11041 gctttgcagg aggcgttgca ggcgcgccgt ttcgccttgc cgaaataccg tatcgaagag
    11101 caaatcggtc atgccaacga cagtatgttt gtgatttcct gcgatttggg cgaactgggt
    11161 ttcgtgtgcc gtgccaaagg gacgagccgt aaggcggcgg agcagtcggc ggcgaaagag
    11221 gcgctgcagt ggctggaaga aaagctgccg ctgaagaaga aaaagaaatg aggcggcgtg
    11281 tgaatatgcc gtctgaaatg tggacatgaa agcgaatatg gatattgaaa ccttccttgc
    11341 agaggaacgt gccgccgacg gttaccgttg cggcttcgta gcgattgtcg gccgtccgaa
    11401 cgtgggcaaa tcaacgctga tgaaccatct catcggtcag aaaatcagca tcaccagcaa
    11461 aaaggcgcag acgacgcgca accgtgttac ggggatttat accgacgata ccgcgcagtt
    11521 cgtgtttgtc gatacgcccg gttttcaaac cgaccagcgc aacgcgctca acgaccgttt
    11581 gaaccaaaac gttaccgagg cactcggcgg tgtggatgcc gtggttttcg tcgtggaggc
    11641 gatgcgcttt accgatgccg accgagtcgt gttgaaacaa ctgcccaagc acacgccggt
    11701 cgttttagtg gtcaacaaaa ttgacaagga caaggcgaaa gaccgttacg cgttggaggc
    11761 gtttgttgcc caggtgcgcg ccgaatttga atttgcggca gcggaggcag tcagcgcgaa
    11821 acacggattg cggattgcca acctgttgga gctgattaaa ccgtatctgc ccgaaagcgt
    11881 gccgatgtat cccgaagaca tggttacgga caaatcggcg cgttttttag cgatggaaat
    11941 cgtgcgtgaa aaattgttcc gctatttggg cgaggaattg ccttatgcga tgaacgtcga
    12001 agtggagcag tttgaagagg aagacggttt aaaccgcatc tatatcgccg ttttggtcga
    12061 taaggaaagc caaaaggcga ttttaatcgg caaaggcggg gagcgtttga agaaaatttc
    12121 caccgaagcg cggttggata tggaaaaact gtttgatacc aaagtgtttt tgaaagtgtg
    12181 ggtcaaagtc aaatccggtt gggcggacga catccgcttc ctgcgcgagc tgggtttgta
    12241 gtttttcttg ctgaacttta cgcaaatgcc gtccgaacag gtttcagacg gcattttgtt
    12301 tcaatcggga atatctttgt taaaaacggg ttgatattat ctgtgca
//
LOCUS       DAWGJV010000051        12239 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWGJV010000051 DAWGJV010000000
VERSION     DAWGJV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.051"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            244..981
                     /locus_tag="WHQ94_001022"
     CDS             244..981
                     /locus_tag="WHQ94_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128316.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIEARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            983..1669
                     /locus_tag="WHQ94_001023"
     CDS             983..1669
                     /locus_tag="WHQ94_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2128317.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPLAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            1828..2691
                     /locus_tag="WHQ94_001024"
     CDS             1828..2691
                     /locus_tag="WHQ94_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128318.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2753..3322)
                     /locus_tag="WHQ94_001025"
     CDS             complement(2753..3322)
                     /locus_tag="WHQ94_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ2128319.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETDYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3481..4341
                     /locus_tag="WHQ94_001026"
     CDS             3481..4341
                     /locus_tag="WHQ94_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ2128320.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     VALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4476..4814
                     /locus_tag="WHQ94_001027"
     CDS             4476..4814
                     /locus_tag="WHQ94_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128321.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNESIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5182..5535
                     /locus_tag="WHQ94_001028"
     CDS             5182..5535
                     /locus_tag="WHQ94_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ2128322.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5525..6391
                     /gene="atpB"
                     /locus_tag="WHQ94_001029"
     CDS             5525..6391
                     /gene="atpB"
                     /locus_tag="WHQ94_001029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ2128323.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6448..6684
                     /gene="atpE"
                     /locus_tag="WHQ94_001030"
     CDS             6448..6684
                     /gene="atpE"
                     /locus_tag="WHQ94_001030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ2128324.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6755..7225
                     /locus_tag="WHQ94_001031"
     CDS             6755..7225
                     /locus_tag="WHQ94_001031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ2128325.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7230..7763
                     /locus_tag="WHQ94_001032"
     CDS             7230..7763
                     /locus_tag="WHQ94_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ2128326.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7774..9321
                     /locus_tag="WHQ94_001033"
     CDS             7774..9321
                     /locus_tag="WHQ94_001033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ2128327.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9346..10221
                     /gene="atpG"
                     /locus_tag="WHQ94_001034"
     CDS             9346..10221
                     /gene="atpG"
                     /locus_tag="WHQ94_001034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ2128328.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10259..11656
                     /gene="atpD"
                     /locus_tag="WHQ94_001035"
     CDS             10259..11656
                     /gene="atpD"
                     /locus_tag="WHQ94_001035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ2128329.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11667..12089
                     /locus_tag="WHQ94_001036"
     CDS             11667..12089
                     /locus_tag="WHQ94_001036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ2128330.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTILADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3007 a         3142 c         3155 g         2935 t
ORIGIN      
        1 ttcaattatt tgccgttttg gtgcgaaccg ctgcctttgc ccgtttcaga cggcattgtc
       61 cgaaatggtt gcctgcttcc tgccttattg acaaaaaaat gctttcccga taatatccta
      121 cgaaaattaa cctgccgatt tgacacagct tgcgggcata acagctaaag cgttccgaca
      181 attccagctt tatcttccgc gcccgttgtg tccgacatcg ggctttgttg tatgggaaag
      241 acaatgatta ttttggacaa ggtttccaag cattaccaaa cgcgcgacaa gacccgtttt
      301 gccgctgtcg agccgaccag cctcgaaatc cgcgacggtg aaatcttcgg gctgatgggt
      361 tattcgggtg caggcaaatc caccctgttg cgcctgatta acctgttgga acgtcccgac
      421 agcggcaagg tcaacgtctg cggacaagag ctgaccgcgc tcgatgccgc cgcattgcgt
      481 caggctcggc agaatatcgg catggtgttt cagcagttca acctgttgaa caaccgcacc
      541 gtcgccggca acgtcgcctt cccattggaa atcgccggat ggccgtctga aaaaatcgaa
      601 gcgcgcgttg ccgaatgtct cgaaatcgtc ggactgaccg aacgcgccgg tcactatccc
      661 gcccagcttt ccggcggaca aaaacagcgc gtcggcatcg cccgcgcgct cgcatccaag
      721 ccgcaagtca tcctcgccga cgaacccact tccgccctcg accccgccac cacgcgcagc
      781 gtcttggaat gtttggaaga catcaacaaa cgcttcaacg taaccatcgt catcgtaacc
      841 cacgaaatga gcgtcatcag acgactgtgc gaccgcgccg ccctgttgga taagggaaaa
      901 gtcgtcgaaa tcgtcgaagt acgcggcaac caaatccacg cccgatccga catcgggcgc
      961 gaactcatca gagaggactg aaatggcaga cctgaccttc caacaagccg tttccaacat
     1021 catcggtatg aaaggcgaaa tcgtccgcgc attgggcgaa accttcgtga tggtcggctt
     1081 gtccaccaca ttcgccgtcg ttttcggcac gttgctgggc gtgttgctct tcgtaacttc
     1141 cagccgccaa ctgcattaca acaagccggt gaacttcctg ctcgacaacc tcgtcaacct
     1201 catgcgcgcc ttccccttcg tcattttgat gattgcgatg atacccgcca cacgcgccat
     1261 cgtcggcagc accatcggcc cgattgccgc ctcgctggtg ttgagcgtgt cgggattgtt
     1321 ttattttgcc cgactggtgg aacaaaacct gcgcgaagtc cccaaaggcg tgattgaagc
     1381 cgccgccgcg atgggtgcgc cgccgcttgc catcgtccgc aaagtcctct taaacgaagc
     1441 gcgcgcgggt atggtttcca gcattaccgt gcttgccatc gggcttttgt catacagcgc
     1501 ggcggcaggg atgataggcg gcggcggctt gggcgacctc gccatccgct acggctacta
     1561 ccgctaccaa accgaagtca tcgtcttcat cgtcgccctc ctcgtgctgc tggtcatcct
     1621 gattcaaagc accggcaaca cgttggcgcg gaaactcgac aaacgttaaa cccgaatgcc
     1681 gtctgaacgc caaaaccccc accgctatcc gaaaaatgct ataaaatccc cctgttcgcg
     1741 gcaaatgccg tctgaacgcc gaatccggac ggcaggactc cctgcccgtc atttttgttt
     1801 gaaactgcca caacatcagg agaaaatatg aaaaccttct tcaaaaccct ttccgccgcc
     1861 gcactcgcgc tcatcctcgc cgcctgcggc ggtcaaaaag acagcgcgcc cgccgcatcc
     1921 gcttctgccg ccgccgacaa cggcgcggag aaaaaagaaa tcgtcttcgg cacgaccgtc
     1981 ggcgacttcg gcgatatggt caaagaacaa atccaagccg agctggagaa aaaaggctac
     2041 accgtcaaac tggtcgagtt taccgactat gtacgcccga atctggcatt ggctgagggc
     2101 gagttggaca tcaacgtctt ccaacacaaa ccctatcttg acgacttcaa aaaagaacac
     2161 aatctggaca tcaccgaagt cttccaagtg ccgaccgcgc ctttgggact gtacccgggc
     2221 aagctgaaat cgctggaaga agtcaaagac ggcagcaccg tatccgcgcc caacgacccg
     2281 tccaacttcg cccgcgtctt ggtgatgctc gacgaactgg gttggatcaa actcaaagac
     2341 ggcatcaatc cgttgaccgc atccaaagcg gacatcgccg agaacctgaa aaacatcaaa
     2401 atcgtcgagc ttgaagccgc gcaactgccg cgtagccgcg ccgacgtgga ttttgccgtc
     2461 gtcaacggca actacgccat aagcagcggc atgaagctga ccgaagccct gttccaagaa
     2521 ccgagctttg cctatgtcaa ctggtctgcc gtcaaaaccg ccgacaaaga cagccaatgg
     2581 cttaaagacg taaccgaggc ctataactcc gacgcgttca aagcctacgc gcacaaacgc
     2641 ttcgagggct acaaatcccc tgccgcatgg aatgaaggcg cagccaaata aggcagtcgt
     2701 ataaaatgat gccgtctgaa ctgtatccgt gttcagacgg catttttgtc ctttaatccg
     2761 ccattccctg ccattccgcc gaatccggcg tatcgattcc gaacagcgac aaagcgtgtg
     2821 caacactgtg cgccactatg tcgtccgccg tctgcggttt gcggtacatc gcaggaacag
     2881 ggggaaacac cacgccgccc atttccgtta cccgcttcat attgtccaaa tgggcaaggt
     2941 tcagcggcgt ttcgcgcacc atcagcacca gccgccgcct ttccttcaaa accacatccg
     3001 ccgcacgcgt cagcagattg tcgccgaagc cgtgcgcgac cgaggcaagc gtccgcatcg
     3061 aacagggggc gaccagcatc ccatccgttt taaacgtacc gctggcaatg cacgccccga
     3121 tattgccgat cggatgcacg aagtccgcca aggcatatac ctcgtctctc gcataatccg
     3181 tttccgaagc gcgcgccatc tccgcacctt tcgataccac aaggtgcgtt tcgatatctt
     3241 gcgcgcgtaa aagttccaaa gccttcacgc cgtattggaa accgctcgcc ccactgatgc
     3301 cgattatcaa acgccgtacc atcatccgcc tttcccataa aaccgcctgc aacggcaaac
     3361 cggctattat agtaaaaaaa cagaaatccg ataaacacgg atacaaattg tcggcaatac
     3421 ccaatatccg ataaaatatc cgatttaaca tcctatctga aataggcacg ggagggcggt
     3481 atggcaaaag taaaaggcgg attggggcgc ggtttggatt cgctgctcgc caacggcgca
     3541 gacaacagca gcggcgaccg attgaccacg gttgcggtta aagatatccg gcccggccgc
     3601 tatcaggcgc gtgttcaaat cgatgacgaa gccttgcagg agctggcaga ttcgattaag
     3661 gcgcaaggcg tgatacagcc cgtcatcgtg cgcgaacacg gactgtcccg atacgaactg
     3721 attgcaggcg aacgccgttg gcgcgccgca cagattgccg gcctgaccga aatccccgcc
     3781 gttatcaaaa ccatcagcga cgaaaccgca ttggcaatgg gtttgatcga aaacctccag
     3841 cgcgaaaacc tcaaccccat cgaagaagca caaggcttga aacgccttgc cgacgagttc
     3901 gggctgaccc acgaaaccat cgcccaagcc gtcggtaaaa gccgaagcgc gatttccaac
     3961 agcctgcgcc ttttaagcct gcccgaaccc gtgcaggaaa tgctttacca acgccgcctc
     4021 gaaatggggc acgcccgcgc attgctgacc ctgcccgtcg tcgaacagct cgaattggcg
     4081 caaaaggccg tcaaaaacgg ctggtcggtg cgcgaagtcg aacgccgcag ccaggtcgcc
     4141 cttcaaaaca aacgtcccga gcccaaaaag actgccgccg ccgacatcgg ccgcctgaat
     4201 gatttgctga ctgaaaaact gggtgtcaac gccgaaatca aaaccgccaa tcataaaaaa
     4261 ggcagaatca tcctgcattt cgacacgccc gaaaccttcg accatatctt gaagcagttg
     4321 ggcatagatt accgacctta attttgcggg atataccgtc tgaaatatag agaacagctt
     4381 tccagatttt aagtgggaaa tataattcta ttgacatttt tctgcttcac gtaagaatcg
     4441 ttttcctgtt ttcattttta attttcgagg aaattatgaa cacacgcatc atcgtttcgg
     4501 ctgcgttcgt tgcgttggca ttagcaggtt gcggctcaat caataatgta accgtttccg
     4561 accagaaact tcaggaacgt gccgcgtttg ccttgggcgt cagcccaaat gccgtaaaaa
     4621 tcagcaaccg cagcaatgaa agcatacgca tcaactttac cgcaactgtg ggtaagcgcg
     4681 tgagccaatg ctatgttacc agtgtaatca gcacaatcgg cgttaccact tccgatgcaa
     4741 tttgtttggg aggcggaacg cacaaaggca aaagtcaatg caatgctttg cttaaagcgg
     4801 caggccgttg ctaatccttt attcggaaaa ggtcgtctga aaatattttc agacgacctt
     4861 ttatttattg agcaaattcc ccaaactgcc gacaatccga ccgataaact gctacaattt
     4921 cgcgttgatt ttttattatt tccacgtaaa gcaaaccaat cacgtaagaa cattaaaaat
     4981 gtaaagaaat tgcggacaaa ctccttaata tcctgtaatt atatgtaatg tgtgttagaa
     5041 tgacacctgt cgccgttatt caggcggcga gcgcggcgga ttgggatacg ttgtttcaca
     5101 aagattaact gtcgcttgac gtaataaggc ttctaaatta tattagccgc gccccaaatc
     5161 cgaggcggaa acattccctt tatgaagcag attatcatcc tacaatccgc cgtattgtcc
     5221 atatgcgccg cagttgcctt tgctgtatgg ggttttgccg gattcctttc ggcggtcggt
     5281 ggcggtttgt cctacctgct tcccacgttt gttgcagttt tacttttaaa acttttcagg
     5341 ggaaacccct tcctgcaaag caggatgttc gtcttcggag agattttaaa agtagtgctg
     5401 tcgctgttgt ccatgcttgc cgtatttgca atatggcatc aatcgctggt gttcgccccg
     5461 tttctgatgg ggctgctcgg tgtcagccat ttggtttttt tagtattgtt gagagtgaaa
     5521 gattatggca ggtgaaacca ttaccgctgc cgactacatc aagcaccact tgcagagctt
     5581 gaccagtttg tcggatgtta ctcagggtca gggactgaaa aacattgctg atttttcgtt
     5641 tattaacctt gatgccgtct tttttgccgt cctgttgggc gtaatcggca gctttctgtt
     5701 gtggcgcggt gcaaaaaaag cgacggcagg cgttcccgga cgtttccagg ccgcagtgga
     5761 aatcttgttt gagtttgtgg acgatatgtg taagagcatc attcacaacg aaaagtctag
     5821 aaaagccgtc gcgccgttgg gtctgacgct gtttgtctgg atttttctga tgaacgcgat
     5881 ggatatgctg ccggtcgatt tgctgccgat ggtatggcag ggcattaccg gcaaccatca
     5941 cgccctgctg cgcgtcgtac cgactgccga tttgaacacc actttggcac tcgctgtcgg
     6001 cgtgttgctg atttgtatct attacaacat caaaatcaaa ggattgggtg gctggtttca
     6061 cgagttgttc agcgcgccgt tcggtgcaaa actcgcacct gcgaacttcc tgttgaattt
     6121 ggtcgagttt ctttctaaaa ccgtatccca cggtatgcgg ttgttcggca atatgtatgc
     6181 gggcgaattg gtattcttgc tgatagcctt gcttggcggt gcttgggcgg cttccggcag
     6241 cgtcgaagtc atggatccga ttctgtttgt attccacatt attgccggtt tggcatgggc
     6301 gattttccat attttggtga ttaccctgca ggcgtttatt ttcatggcgt tggcgttcgt
     6361 ctatatcgga caggcacatg atgcgcactg attttcctaa gtagtttgtt tttgtagttt
     6421 aacctttgtt tctttaagga gtttaaaatg ggtttgattg ctatcgcatg tggtttgatc
     6481 gttgcattgg gtgcattggg tgcctctatc ggtatcgcaa tggtcggttc taaatatttg
     6541 gagtcttctg cccgtcagcc cgaactgatc ggtccgctgc aaaccaaact gttcctgatt
     6601 gccggtttga ttgatgccgc attcttgatt ggtgtcgcca ttgcactgct gttcgccttc
     6661 gttaacccgt ttgcaggtgc ataatcagga cggattcgtc cgttccgtac aggcgcagcc
     6721 tgtttgatta accccagtac gaaggttaag taacgtgaat atcaatgcaa cattattcgc
     6781 tcaaatcatc gtctttttcg gtttggtatg gtttaccatg aaattcgtgt ggccgccgat
     6841 tgcaaaagct ttggatgagc gtgccgcaaa agtcgccgaa ggcttggctg ccgccgagcg
     6901 tggtaaaagc gatttcgagc aggctgaaaa aaaggttgca gaactcttgg cagaagggcg
     6961 taatcaggtt tccgaaatgg ttgccaacgc cgaaaaacgt gccgccaaaa ttgtcgaaga
     7021 agccaaagaa caggcttctt ccgaggcggc gcgcattgca gctcaggcaa aggccgatgt
     7081 ggagcaggaa ttgttccgcg cacgcgaatc cctgcgcgag caggttgccg tgttggctgt
     7141 caaaggtgcc gaatctattt tgcgcagcga agtcgatgct tccaaacacg caaaactgct
     7201 cgataccctg aaacaggagc tgtaatctta tggcagagtt cgcaacgatt gccagacctt
     7261 atgcaaaggc attgttcggt ctggctcagg aaaaaaacca aattgagtct tggttgggcg
     7321 gactggaaaa acttgcggcg gttgttcagg aagggaaggt ggcttcattg attgaccgtc
     7381 ctgaaacgaa tgcttcagaa aaagcagata tcctcatcga tttggtcggt ttgaaagaca
     7441 aggagttgaa aaactttgtt attgtcttgg ccgggcagaa acgtttgtcg atattgccgg
     7501 aagtatatgc tcaatatcaa gacttgacct tatcattcaa ccatatcaaa tctgccgtca
     7561 tttacagtgc ctatccgttg accgacaaac aggtcggcga gttggcgcaa atgctgaata
     7621 agcgtttcga cagcgagctg aaaatctctg tcgaaatcga accggagctg attggcggca
     7681 taaaagttga agtgggtgat caggttttgg atttgtctgt acaaggcaaa ttgagtgcct
     7741 tgtacacgac tatgacgaat taggagagtt ttcatgcagc ttaatcctgc tgaaattagc
     7801 gatttgatta aagccaagat cgaaaatctg tctgtaaatg ccgaagtgcg tacccgtggt
     7861 acggtgattt cggtaactga cggtatcgtt cgcatccatg gtttgtcaga tgcaatgcaa
     7921 ggtgagatgc tcgaattccc gggtaacact ttcggcttgg cgatgaacct ggagcgcgac
     7981 tccgtcggcg ccgtagtgtt gggcgagtac gaacatatta aagaaggcga cacggttact
     8041 tgtaccggtc gtatcttgga agtgccggtc ggacgcgaac tggtcggacg cgtcgttgat
     8101 gcattgggtc gtccgattga cggcaaaggc ccgattaata caactttgac cgcccctatc
     8161 gaaaaaatcg caccgggcgt gattgcgcgc aaatcggttg accagccgat gcaaaccggt
     8221 ctgaaggcga ttgactctat ggttcctgtc ggtcgcggtc agcgtgagtt gattattggc
     8281 gaccgtcaga caggtaaaac ggccgtagca ttggatgcca tcgtcaacca aaaaggtacg
     8341 ggcgttatct gtatctatgt cgctatcggt caaaaagcat cttctattgc caacgtggtc
     8401 cgcaaattgg aagagcatgg cgcgatggaa cacaccatcg tggttgctgc aactgcatct
     8461 gaagcggcgg cattgcaata tatcgcacct tactccggtt gtacgatggg tgaattcttc
     8521 cgcgaccgcg gcgaagatgc cttgattgtt tatgacgatt tgtccaaaca ggctgtggct
     8581 taccgtcaaa tttccctgct tttgcgccgt ccgcccggcc gcgaagccta tcccggcgat
     8641 gtgttctacc tgcattcccg tctgttggaa cgtgcagcac gtgtcaatga acacgaagtg
     8701 gagaaattga ccaacggcga agtaaaaggc aaaaccggtt ctctgaccgc gttgccgatt
     8761 atcgaaaccc aagcgggcga cgtatctgct ttcgttccga ctaacgtcat ttcgattacc
     8821 gacggtcaga ttttcttgga aaccgacctc ttcaacgccg gtatccgtcc tgcaatcaat
     8881 gccggtattt ccgtatcccg cgtaggcggt gctgcacaaa ccaaagtgat taaaaagctg
     8941 ggtggcggta tccgtttggc gttggcgcaa tatcgtgaat tggcggcttt ctcgcaattt
     9001 gcatccgatt tggatgaagc tacgcgcaaa caattggagc atggcgaagt cgtaaccgaa
     9061 ctgatgaaac agaaacagtt cagtacgttg aatacggctg aaatggcttt gaccctttgg
     9121 gcaatcaaca acggttcgta ttctgatgtt ccggttgcca aagctctggc ttttgaatct
     9181 gaatttttga gctttgtccg tacccaacat ccggaagttt tggaagccgt caatgcttca
     9241 ggtgcaatgt ccgacgagag cgagaaaacg cttgaagcag ccatgaaatc cttcaaatct
     9301 tcttacgcct atcaggcata aggcttgaag tgaaaggagt ctgaaatggc agtaggaaaa
     9361 gagattctca ccaaaatccg cagtgttcag aatacccaaa agatcactaa agcgatgcag
     9421 atggtgtcaa cctctaaaat gcggaagact caggaacgga tgcgtttggc gcgtccgtat
     9481 gccgaaaaag tgcgtatggt gatgagccat cttgcgcaaa ccaataccga tcatggtatt
     9541 ccgttactgg aatctcatcg ggaaatcaga cgtgtcggtt ttattttgat tacgtctgat
     9601 aagggtttgt gtggcggttt gaacgccaac gtgctgaaaa agtttttggc acaagttcaa
     9661 gagtatcgga atcaaggtat tgaagaggaa atcgtatgcc ttggcagtaa aggtctgatg
     9721 gcgtgtcaga gcattggtct gaatgtggtt gccagtgccg taaatttggg cgatacccca
     9781 aaaatggaaa tgctgctcgg acctttgaca gaactcttcc aacggtatga gaaacatgaa
     9841 attgacagaa tccatctggt gtattcgggt tttgtcaata ccatgcgtca agaaccgaga
     9901 atggaagtat tgctgcctat cggtgagaac gtgattggcg attcagctcc caaatcaccg
     9961 ttcagctggg aataccgcta cgaaccgact gcacttgcag tgttggaata tctggttcgc
    10021 cgctatttag agtctgtggt ttatcaggcg ttgagcgaca atatggcatc cgaacaggca
    10081 gcccgcatgg ttgccatgaa agctgcaaca gacaatgcag gcaatgccat caaagagttg
    10141 cgtttggtat ataacaaatc gcgtcaagct gcgattacca cggaattgtc agaaattgta
    10201 gcaggtgcgg cggctgtctg atgccgtctg aatcctgtac ggaaattagg atacgataat
    10261 gagccaaggc aaaatcgtac aaattatcgg tgcggttgtt gacgtggaat ttccacgcga
    10321 catgattccg cgcgtttacg acgctttgaa attagacgaa aacggtctga ctttggaagt
    10381 ccaacagctt ttgggcgacg gcgtagtccg tgccattgcg atgggcagct cggacggttt
    10441 gaaacgcggc atgactgtga gcaataccgg tgcgcccatt actgtgccgg taggtaaagg
    10501 tacgttggga cgcattgtcg atgtattggg aacgcctgtt gacgaagcag gtccgatcga
    10561 taccgacaag agccgtgcca tccaccaagc tgcccctaag tttgacgaac tgtcttccac
    10621 aaccgaattg ctcgaaacgg gcattaaagt gattgacttg ctgtgtccgt ttgccaaagg
    10681 cggtaaagta ggtctgttcg gcggtgccgg tgtgggtaaa accgtgaaca tgatggaatt
    10741 gatcaacaac atcgccaaag cgcacagcgg cttgtccgtg ttcgcaggcg tgggtgagcg
    10801 tacccgcgaa ggtaacgact tctaccacga gatgaaagat tccaacgtat tggataaagt
    10861 ggcaatggtt tacggtcaga tgaacgaacc tccgggcaac cgtttgcgcg tcgcattgac
    10921 cggtttgacc atggcggaat acttccgtga cgaaaaagac gaaaacggca aaggccgcga
    10981 cgtattgttc ttcgtggaca acatctaccg ttacaccctg gctggtaccg aagtatccgc
    11041 attgttgggt cgtatgccgt ctgcagtggg ctaccaaccg acattggcag aagaaatggg
    11101 tcgtttgcag gagcgtatta cctctaccca aaccggttct attacttcca tccaagccgt
    11161 atatgtacct gcggacgact tgaccgaccc gtctccggca acaacgttcg cccacttgga
    11221 cgcaaccgtc gtattgagcc gcgatattgc ctctttgggt atttacccgg cagtcgatcc
    11281 gcttgattct acttcgcgcc aactggatcc gatggtattg ggtcaagagc actacgacgt
    11341 agcgcgcggc gtacagtcca ccctgcagaa atacaaagaa ttgcgcgaca tcatcgccat
    11401 cttgggtatg gacgaattgt ctgacgaaga caaactgacc gtaatgcgtg cgcgtaaaat
    11461 ccaacgcttc ctgtcccaac cgttccacgt tgccgaagtg tttacaggtt ctccgggcaa
    11521 atatgtcgcc ctgcgcgata ccattgccgg cttcaaagcc atcttgaacg gcgaatacga
    11581 ccatctgccc gaacaggcat tctacatggt cggcagcatc gaagaagcgg ttgagaaagc
    11641 gaaaacctta aactgaggag gtcggcatga gcatcatgca agttgaggtg gtcagtagcg
    11701 agcaaaacat ctattcgggc gaagccagct ttgtagtggt tccgaccgtt caaggtgagc
    11761 ttggtattta cccgcgacac gagccgatta tgagtttggt gcggccgggg gctttgcgtt
    11821 tgaccgttcc gggcgaggat aaagaggttt tggttgctgt ttccggcggt gttttggaag
    11881 tacagcctga taaagtaact atcttggcgg atgttgccgt ccgcagtgcg gagatggatc
    11941 aggcacgtgc ggaagaggcg aaaaaagccg cagaagcggg catttcccaa gctaaagacg
    12001 ataaggcttt ggcggaggcg cataaagcat tggctgctgc gattgcacag ctcaaaactt
    12061 tggactatat ccgttcgcac aagaaataaa cttgtttggt ttggaaaagc acgaccgttt
    12121 ggtcgtgctt tttattttcc ggatttaaat taaagactgt gtttggatat ggcgttggca
    12181 ggcatagcct gctttaaggt attcaatagg gcagcctgcc gcctaaccga aacatatag
//
LOCUS       DAWGJV010000052        12124 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWGJV010000052 DAWGJV010000000
VERSION     DAWGJV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.052"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(59..988)
                     /locus_tag="WHQ94_001037"
     CDS             complement(59..988)
                     /locus_tag="WHQ94_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2128331.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1119..1868
                     /locus_tag="WHQ94_001038"
     CDS             1119..1868
                     /locus_tag="WHQ94_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ2128332.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAMEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1991..2572
                     /gene="petA"
                     /locus_tag="WHQ94_001039"
     CDS             1991..2572
                     /gene="petA"
                     /locus_tag="WHQ94_001039"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ2128333.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2591..3940
                     /locus_tag="WHQ94_001040"
     CDS             2591..3940
                     /locus_tag="WHQ94_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ2128334.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            3955..4743
                     /locus_tag="WHQ94_001041"
     CDS             3955..4743
                     /locus_tag="WHQ94_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ2128335.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5082..6866
                     /gene="rmuC"
                     /locus_tag="WHQ94_001042"
     CDS             5082..6866
                     /gene="rmuC"
                     /locus_tag="WHQ94_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ2128336.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7073..7705)
                     /locus_tag="WHQ94_001043"
     CDS             complement(7073..7705)
                     /locus_tag="WHQ94_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ2128337.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7796..8620
                     /locus_tag="WHQ94_001044"
     CDS             7796..8620
                     /locus_tag="WHQ94_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ2128338.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGMSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8689..9252
                     /locus_tag="WHQ94_001045"
     CDS             8689..9252
                     /locus_tag="WHQ94_001045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ2128339.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9334..9771
                     /locus_tag="WHQ94_001046"
     CDS             9334..9771
                     /locus_tag="WHQ94_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ2128340.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9840..10109
                     /locus_tag="WHQ94_001047"
     CDS             9840..10109
                     /locus_tag="WHQ94_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ2128341.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10109..11884
                     /gene="ptsP"
                     /locus_tag="WHQ94_001048"
     CDS             10109..11884
                     /gene="ptsP"
                     /locus_tag="WHQ94_001048"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ2128342.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
BASE COUNT         2944 a         3069 c         3409 g         2702 t
ORIGIN      
        1 tgttaatcca ctatacatga aaaaatagaa aaactgccgg gtatggttga ataaggggtc
       61 agaccggttc caattcgctc agtccgggca aatctgcaaa accgcgttcg cgtatgattt
      121 ggcaaaaatt gttcagatag ctcttgtccg tatcttcggt acggatggcg gcatacagtt
      181 tgctttgcaa tccgtcggca gtaatttggc ggtggacgac atagcctttt tcaaggtagg
      241 gcatgactgt ccaataggga agggcggcaa tgccacgtct gctggcaacc agttggataa
      301 tggcgatggt cagttcgctg tgtcggcgcg gcgggttgat gtttttcgga atcaggattt
      361 ttttgggcaa atccagcatc tcgtcgggaa cgggataagt aatcagggtt tccccgataa
      421 agtcttccgc cgtccaaacg tttttggcgg caagcggatg gtctggtgcg caaatgccga
      481 ccatttcgta ggcaaacagc ggttggaaag aaatatcgtt ttgtttttcc gcttcggaaa
      541 caatggcaag gtcggcacgg tgttgcagca gcagtccgac gggatccgct tggaatcccg
      601 atacgatatc caattcgact tggggccaca tcgggcggaa ttcgcccatg gcgggcatca
      661 gccagtcgaa acaggtatgg cattcgacgg caatccgcag ctctcccgcc tctccttccg
      721 tgattcgcgc caaatcccat tctgcaacag caacttgagg tataagttcg tgggcgaggc
      781 gcagcagcct ttcgcccacc ggggtaaagc gcaagggcgt ggatttgcgt tcgaacagcg
      841 gcgtgccgta gtggttttcg agcatacgga tctggtggga aagagcggat tgggtaagga
      901 aaacccgttt ggcggcaagg gagacgctgc cggtttcttc aagtgccagc agggtcttga
      961 ggtggcgcag ttcgataatg gaatccatgg ggcttcagac ggcatattga accggcgcat
     1021 attaaaataa ttcatatgcg ggtgcaaatc ggaaaaatgt gaattccgga cattttgcga
     1081 ttagaatgcc cgcttgttta aagcgattag gaaaaaagat ggtattgcgc agggattttc
     1141 tgacttggtg taatgaaaca ttgcagacag cgttgtttaa agattacgcc cctaacggtt
     1201 tgcaggttga agggagggaa tatatcggga aaatcgttac gtcggtaacg gcaagcaggg
     1261 cagcgattga ttttgctatg gagcagaagg cagatttgct tttggtacat cacggtatgt
     1321 tctggaaaaa cgagttgccg accgttactg gttggaaaaa agaacggatt gccgcactgt
     1381 tacggcacga catcaatatg gcaggctacc atctgcccct ggatgcacat cccacactgg
     1441 gcaacaatgc ccaactcgcc gacagattgg gttttgcgac agaaaaacgg ttcggcgaac
     1501 aaaacctgct caactcgggc agcctgaaac aagccaagac actcggcgca ttggcggcgc
     1561 atattgaaac agttttgcaa cgtaagcctg tcgtcatcgg caaacccgaa cgcgaaatcc
     1621 gacgggttgc atggtgcagc ggcggggcgc aggggttttt tcagacggca atagacgaag
     1681 gtgtcgatct gtatttgacg ggggaaattt ccgaagccca ataccacctt gccaatgaaa
     1741 cgggtacggc tttcatttcg gcagggcatc acgcgacgga acgttacggc gtacgcgcgc
     1801 tggcagaatc ggcggcagag gttttcgggt tggaagtgtg ccattttgac gaaaacaatc
     1861 cggcttgagt cttgagaaat atcataaaac tttaccttat tttaaataat gctttgagta
     1921 tcactgcaaa ctgggtaaaa ttgcaacgtt taatggctcg gtattcccaa aatattagga
     1981 cgactgaata atggataatc aagaaatcaa caacggccgc cgccgtttcc tgacactcgc
     2041 gacctgcggc gcgggcggag tggcagcatt gggtgtggca acgccgtttg tggccagttt
     2101 tttcccttcg gaaaaagcca aggccgccgg tgctgccgtc gaggtggatg tcagtaaaat
     2161 cgaagcgggt cagctgctga ccgccgagtg gcaaggcaaa ccgatttggg tgctcaaccg
     2221 tacagatcag cagcttaaag acctgaaagg cctgaacggc gaacttaccg atcccaattc
     2281 cgatgcggaa cagcagccgg agtatgctaa aaacgagacc cgttcgatta agccgaacat
     2341 ccttgtcgcc atcggtatct gcacccattt gggctgctcg cccaccttcc gtcccgacat
     2401 tgcccccgcc gatttgggtg cagactggaa aggcggcttc ttctgcccct gccacggttc
     2461 gaaattcgac ttggccggcc gcgtatataa aggtgttcct gccccgacca acctggttgt
     2521 cccgccatat aaatacttga gcgacacaac tatcttggtg ggcgaagact aagaataagg
     2581 aacgataatt atggcaaacc aaaccaatag caaagcaaaa gcattgttag gctggatgga
     2641 cgctcgtttc cccctgtcta aaatgtggaa tgagcatttg gctcaatact acgcaccgaa
     2701 aaactttaat ttttggtact acttcggctc tttggccctg cttgtcctcg tgattcaaat
     2761 cgtcagcggc attttcctga ccatgaacta caaacccgac ggcaacctga acgcctacca
     2821 tctgcctgct gcctttactg cagtcgagta catcatgcgc gacgtgtccg gcggttggat
     2881 tatccgctac atgcattcca ccggcgcatc tttcttcttc attgttgttt atctgcacat
     2941 gttccgtggt ttgatttacg gttcgtacaa aaaaccccgt gagttggtgt ggattttcgg
     3001 ttctctgatt ttcttggcat tgatggcgga agcctttatg ggctacctgc tgccttgggg
     3061 tcagatgtcc ttttgggggg cgcaggtaat tattaacctg ttctccgcca tccctgttat
     3121 cggtccggat ttgtccactt ggattcgcgg cgacttcaac gtttccgacg ttaccctgaa
     3181 ccgtttcttt gccctgcacg ttatcgcggt acctctggta ttgctcggct tggttgtggc
     3241 gcacatcatc gccctgcacg aagtgggttc taacaaccct gacggcgttg aaatcaagaa
     3301 aaacaaagac gaaaacggta tcccgcgcga cggtatccca ttccatcctt actataccgt
     3361 taaagacatc ttgggtgtgg ttgtattcct gattgtcttc tgtgccgtat tgttcttcgc
     3421 accggaaggc ggcggctact tcctcgaagc gccgaacttc gatgcggcaa atgcgctgaa
     3481 aacacccccg cacattgcgc cggtatggta tttcacaccg ttctacgcca ttttgcgtgc
     3541 gattccctcg ttcttgggaa cgcaggtttg gggtgtaatc ggtatgggcg cggcggtcgt
     3601 gttgattgcc ctgctgcctt ggctggatag gggcgaggtg aaatccgtcc gttaccgcgg
     3661 tccgattttc aaaaccgcat tggtcttgtt catcatttcc tttatcggtt tgggtatttt
     3721 gggcgcgatg gtggcaaccg atacgcgtac tttggtcgcg cgcatcctgt ccttcgtcta
     3781 tttcgcattc ttcctgggta tgccgttcta taccaagctg gataaaaaca aaccggttcc
     3841 cgaacgcgta accatgagca ccgccaaaca aaaaatcatg ttctttgttt acgtcggcat
     3901 taccgccgtg ggtgcttacc tgtttgcaac caacatctga tgagggcagt caaaatgaaa
     3961 caagctatga aaaattggtt tgctgccttg ctgttggcag tacccatggg tgcggcattt
     4021 gcttcgggcg gtcatgcaca ctatgagaaa gtcgatatcg atttgcgcga ccaagtcagc
     4081 ctgcagcgcg gcgcacaaat ctttaccaac tattgcctgt cctgccactc ggcaagcggt
     4141 atgcgtttca accgtttgaa agacatcggt ttgactgacg aagaaatcaa gaaaaacctg
     4201 atgtttacca ccgataatgt cggcgatgtc atgcattcgg cgatgaaccc gaaagatgcg
     4261 gcaaaatggt ttggtgctgc tccgcccgat ttgacattga ttgcgcgttc caaaggtgca
     4321 gactaccttt acgcttatat gcgcggcttc tataaagatc cgacccgtcc gagcggctgg
     4381 aacaatactg tattcgataa agtcggtatg ccccacccgt tgtgggaaca gcaaggcgtt
     4441 caagccgttg agttggatgc caaaggtcag ccggttatgg taaaagacga acacggcgag
     4501 atgaagccta agctgtattg ggaatctacc ggtttgcaca gccgccgtct gcctaacggc
     4561 aaagtgatcc aaaaagagta cgacgcatat gtacgcgatt tggtcaatta ccttgtgtac
     4621 atgggcgaac ctgcacaact gcaacgcaaa cgtataggct atgtcgtgat gattttccta
     4681 tttgcggtta tgctgccttt ggcgtatttc ctgaaaaaag aatattggaa agacgtacac
     4741 taagcgtttg gaacaaaagg gcaaatcctt tagggtttgc ccttttttca ttttgcctgc
     4801 cgtttgaaaa gcctaaatcc gtatgccgtc tgaacatagc tgcaacattt cagacggcat
     4861 cattctaaaa atgtcagaca tcgggaactt aatcagggtt tgtggcagat tgatattgaa
     4921 ttaggaaaag gtctgaaacc gcaagcagca tggtaaggcg atgtcgaaca ggtttaaagc
     4981 caatccgcta tattttttgg agctatgtcg aacaaacagg tagaatcgga ataagcggcg
     5041 gttttgtccg gtgtgaaaat gtttgaatat taggatgatt gatggaactg atgactgttt
     5101 tgctgccttt ggcggcgttg gtgtcgggcg tgttgtttac atggttgctg atgaagggca
     5161 ggtttcaggg cgagtttgcc gatttgaacg cgcaactggc ggaaaaggcg gcaagatgtg
     5221 attttgtcga acaggcacac gcagaaattg catcggaatt ggccgttttg gacgggaaat
     5281 accgacattt gcaggacgaa aattatgctt tgggcaaccg tttttccgca gccgaaaagc
     5341 agattgccca tttgcaggaa aaagaggcgg aatcggttcg gctgaagcag tcgtatatcg
     5401 agttgcagga aaaggcacag ggtttggcgg ttgaaaacga acgtttggca acgcagctcg
     5461 gacaggaacg gaaggcgttt gccgaccaat atgccttgga acgccaaatc cgccaaagaa
     5521 tcgaaaccga tttggaagaa agccgccaaa ctgtccgcga cgtgcaaaac gaccttgccg
     5581 atgtcggcaa ccgttttgcc gcagccgaaa aacagattgc ccatttgcag gaaaaagagg
     5641 cggaagcgga gcggttgagg cagtcgcata ccgagttgca ggaaaaggca cagggtttgg
     5701 cggttgaaaa cgaacgtttg gcaacgcaaa tcgaacagga acgccttgct tctgaagaga
     5761 agctgtcctt actgggcgag gcgcgcaaga gcttgagcga tcagtttcaa aatcttgcca
     5821 acacgatttt ggaagaaaaa agccgccgtt ttaccgagca gaaccgcgag cagctccatc
     5881 aggttttgaa cccgctaaac gaacgcatcc acggtttcgg cgagttggtc aagcaaacct
     5941 atgataaaga atcgcgcgag cggctgacgt tggaaaacga attgaaacgg cttcaaggat
     6001 tgaatgcgca gctgcacagc gaggcaaagg ccctgaccaa cgcgctgacc ggcacgcaga
     6061 acaaggttca gggtaattgg ggcgagatga ttctggaaac ggttttggaa aattccggcc
     6121 tccagaaagg gcgcgaatat gtggttcagg cggcatccgt ccgaaaagag gaagacggcg
     6181 gcacgcgccg cctccagccc gacgttttgg tcaacctgcc cgacaacaag cagattgtga
     6241 ttgactccaa ggtctcgctg acagcttatg tgcgctacac gcaggcggcg gatgcggata
     6301 cggcggcacg cgaactggcg gcacacgttg ccagcatccg tgcacacatg aaaggcttgt
     6361 cgctgaagga ttacaccgat ttggaaggtg tgaacacatt ggatttcgtc tttatgttta
     6421 tccctgtcga accggcctac ctgctggcgt tgcagcatga cgcgggattg ttccaagagt
     6481 gtttcgacaa acggattatg ctggtcggcc ccagtacgct gctggcgact ttgaggacgg
     6541 tggcgaatat ttggcgcaac gaacagcaaa atcagaacgc actggcgatt gcggacgaag
     6601 gcggcaagct gtacgacaag tttgtcggct tcgtacagac gctcgaaagc gtcggcaaag
     6661 gcatcgatca ggcgcaaagc agttttcaga cggcattcaa gcaacttgcc gaagggcgcg
     6721 ggaatctggt cggacgcgcc gagaaactgc gtctgttggg cgtgaaggca ggcaaacaac
     6781 ttcaacggga tttggtcgag cgttccaatg aaacaacggc gttgtcggaa tctttggaat
     6841 acgcggcaga agatgaagca gtctgacttg tgcggaaaaa tattgtttca gccggggcgg
     6901 gaatgccgaa agcgcggcgt aactgtactg gttctgattt tgggtttgtt tgaagtactt
     6961 accaaagcct tgtccggcga ggtacaccgg caaggcggcg gttgtctgag ccttttgtgt
     7021 ttcagacggc atcagtgcag atgatgccgt ctgaagaccg tagggaaggc ggttagaaaa
     7081 acggattgtg ccgtttttcg tgtccgatgg aagtcatacg cccgtgtccg gcgacaactt
     7141 gcacggtttc gggaagggtg aataatttgt tgcggatatt attgattaag tcggcgtggt
     7201 tgccgcgcgg aaaatcggtt ctgcctatgg tttcgtaaaa cagcacgtcg cccgcaatca
     7261 gcaattccgc ctcggcacaa taaaagacga tatgtcccgg cgtatggccc ggaatatgca
     7321 gcacttgaaa ggcatagcgt ccgaccgtga gcgtttcgcc ttcttcgagc caacggttcg
     7381 gcgcaaaggc gggcgagacg ggaaatccgt attgcgcggt ggtttgcggc agcgattgga
     7441 gcaggaattc atcgtccgga tgcggcccga ggacagggac tttatgcgtt ttcaacattt
     7501 cgaccacgcc gcccgcgtga tcgagatggc cgtgcgtcag ccagattgcc gtgagcgtaa
     7561 gtttgcggtt tgccaacgct tgcagcagga acggcacgtc gccgccgaca tcggtcagga
     7621 cggcttcgcc gctttcgtcg tcccaaatca gggtgcagtt ttggcggaag ggggtaacgg
     7681 ggaagatttc gtaacgtagg gtcatcggcg tgttcctaaa cggttttcag acggcatcgg
     7741 gtttgccgtt tgttttatgg cggtttgccg cctgtttgga cattggggga atcgggtgat
     7801 taagaagaca atcggcggca tcataccgat ttttatggcg gttttcatcc ctgcatcggc
     7861 aggcgcggcg gatttgatgc tggcgcagga atacaaaggg caggatattg ccggctgggc
     7921 gatgagcgag aaactcgacg gcgtgcgcgc gtattgggac ggaaagcacc tgataagccg
     7981 tcagggctat gcgtttacgc cccccaaagg ttttaccgct cagtttccgc cttatccttt
     8041 ggacggcgaa ttgtatagcg gacgcggtca gttcgagcag atttccgccg ccgtgcgttc
     8101 tgcttcttca gattggcgcg gcatccgcct gcacgttttc gatgtaccca aggcgcaggg
     8161 caacctctac caacgtttgg cagtcgcaac gcagtggctg aaaacgcatc cgaacgcgcc
     8221 gattaccatc atcccgcaaa tcaaagtgcg cgaccggcag cacgcgatgg actttttaaa
     8281 acaaatcgaa gcgcagggcg gcgaaggcgt gatgctgcgt cagcccgaat cccgttacag
     8341 cggcggcatg agcagccaat tattgaagct gaaaagccaa tacgacgacg aatgcacggt
     8401 aacgcggcac tatgagggca aagggcgaaa cgccggacgg ctgggcgcgg tcggctgcaa
     8461 aaaccgacac ggcgaattcc gcatcggcag cggtttcaag gacaaagacc gcgacaaccc
     8521 gcccaaaatc ggcacgctga ttacctaccg ttaccgtggc tttacgcgga aaggcacgcc
     8581 gaaatttgcc acatttgtgc gcgtgcgtac cgaccgctga gcaggacagt tcagacggca
     8641 gccgatactt tggtttataa ttttcctttt accgaccgat tccgacatat gaccgattta
     8701 gaaaccaaac gccttgaaac acaggcgatg cttgaaaacg ctgatctttt gttcgaccaa
     8761 ggccaatgcc gtgccgcact gcaaaaagtg gcggacgaga ttacgcgcga tttgggcggc
     8821 aaatatccgc tgctgctgcc cgtgatgggc ggcgcggtgg tgtttacggg gcagttgctg
     8881 ccgctgttgc gttttccctt agattttgat tatgttcacg tttcccgtta cggcgacaag
     8941 ctggagggcg gcgcgttcaa ctggaagcgt atgcccgatg cggaacaaat ccggggcagg
     9001 cacgtcgtcg tgctggacga tattttggac gaagggcata cgatgtccgc cattcaagcc
     9061 aaacttttgg aaatgggtgc ggcaagctgc cgtgcggcgg tgttcgccaa caagctgatt
     9121 gacaaaccca agcctatccg agccgattat gtcggactgg atgtgccgaa ccgttatgtt
     9181 ttcggttacg gcatggatgc ggcgggctgc tggcgcaatc tgggcgagat ttacgcattg
     9241 gacggaaaat aaggacgcga tgccgtctga aggcctttca gacggcatcg cgcccatacg
     9301 ccggcaggat aatgaagaac aggacgcatc aatatgatag ggcttttaat catcacacac
     9361 gaaaccatag gcgaagccta ccgcaagctg gcacatcatt ttttcccggg cgggctgcct
     9421 gaaaacgtcc gcatactcgg cgtgcaaccg acggaagacc aagacgacat catcaacaac
     9481 gccattgccg cgcttcagga atttcccgac aacgacggcg tgctgattat gaccgatatt
     9541 ttcggcgcga ccccctgcaa tgcagcccgc cgcctcgtgc gcgaaaacaa atcggcgatt
     9601 ttgaccggac tgaacgcgcc gatgatggtt aaggccgtcc aatattcgcc ggcggcggaa
     9661 gaccttgccg cctttaccga atgcgtcagg gaggcggcgg taaaaggcat tttcgccatc
     9721 acgtccgcgc ccgaagattt ggtgtgccgg cgcagcggcg atgccgtctg aagaagcggc
     9781 agggcaggaa aacattttaa acggcgtctg ctgccgatat acataacacg ggaatcgaaa
     9841 tgctcaaaca atccatcgaa atcatcaaca aactcggact tcacgcccgt gcctccagca
     9901 aattcaccca aaccgcgtct cagtttcaaa gcgaagtctg ggtaacgaaa aacggcagcc
     9961 gcgtcaacgg caaaagcatc atggggctga tgatgctcgc cgccgccaag ggtacggtca
    10021 tcgagctgga gacggacggc gcggacgagg cggaagcgat gcgcgccctg accgacttaa
    10081 tcaacggcta cttcggcgag ggcgaataat gagtatcgtg ctgcacggcg tggcggcggg
    10141 caaaggcatt gccgtcggtt gcgcccacct gattgcgcgc ggtacggagg aagtgccgca
    10201 gtatgatgtt gcggaggcgg acaccgatgc cgaagccgaa cgtttcgatg ccgccgtcaa
    10261 agccacgcgc aaagagttgg aacagctccg cagcgcgatt cccgaaaacg ccccgaccga
    10321 gttgggcgcg ttcatctcgc tgcacctgat gctcttgacc gatgttacct tgtcgcgcga
    10381 acccgtcgat attttaaggg aacaaaaaat caacgccgag tgggcattga agcagcagag
    10441 cgacaaactc gccgcccaat tcgacaatat ggacgatgcc tatttgcgcg aacgcaagca
    10501 ggatatgctg caagtcgtcc gccgcatcca caacaacctg atcgggcagg gcaacgagtt
    10561 ggaagttgcc gacaacctgt ttgacgaaac cgttctgatt gcaaacgacc tttcgcccgc
    10621 cgacacggtt ttgtttaaag agcagcgcat tgccgccttc gttaccgatg ccggcggccc
    10681 caccgggcat acggcgattt tgggcaggag cttggacatc ccgtccgtcg tcgggctgca
    10741 caacgcgcgc aaactgatta ccgagggcga aacggtcatt gtggacggta tcaacggcgt
    10801 gttgattatc gcgccggatg agtcggtgtt gaacgaatac cgccgccgtg cccgcgaata
    10861 ccgcagccac aaacgcgatt tgaacaagct caaaaaaacc gccgccgcca ccgctgacgg
    10921 ggtctgcatc gagcttgtgg gcaatataga atccgccgaa gacgtgaaac cgctgcacaa
    10981 cctcggcgca gacggcatcg ggctgttccg cagcgagttt ctttacctga accgcgatac
    11041 gatgccgtct gaagacgagc agtacgaagt gtacagcgcg attgtcaaaa aaatgaaagg
    11101 caaaagcgta acgatacgga cagtcgattt gggtgtggac aaaaacccgc gctggttcgg
    11161 gaaaaacagc acgcccaacg gcagcctcaa ccccgcgctg ggcatgaccg gcatccgcct
    11221 gtgccttgcc gaaccggtca tgttccgcac ccagatgcgc gccatcctcc gcgccgccgc
    11281 ccacggcccc gtgcggatga tgtggccgat gattacctcc gtatccgaag tgcgccagtg
    11341 cctcatccac ctcgacaccg cgcaacgcca gcttgccgaa cgcggcgatg ccttcggtaa
    11401 agtcggcatc ggctgtatga ttgaaattcc gtctgccgcg ctgaccgtcg gcagtatttt
    11461 gaaactggtc gatttcatct ccgtcggcac caacgacctg attcaataca tcttgtccgt
    11521 cgatcgcggc gacgacagcg tcagccacct ctaccagccc ggccatcccg ccgtgctgaa
    11581 aatgctgcaa cacgtcatcc gcaccgccaa ccgcatggac aaagacgtgt ccgtatgcgg
    11641 cgagatggcg ggcgatacgg cgtttacccg catcctgctt ggtatggggc tgcgccgttt
    11701 ttccatgaac cccaacaaca tcctgcccgt caaaaacatc atcctgcaca gcaatgtcgg
    11761 acagctcgaa agcgatattg cgaaagtcat ccgctgcgaa gacgaagaga agtcggaaaa
    11821 gctgatcaaa cagatgaaca gcgtgtctgt cgaggaagaa gccgacttca aggggcggaa
    11881 ataaatacgg caggtaaaaa atagaaatac ttaacaatgc ccgcaatctg aaattttgcc
    11941 attcttgcaa aatagaaaac cgaaacagaa acccaaaatc ggtcattccc tcaaaaacag
    12001 aaaaccaaaa tcagaaacct aaaatccgtc attcccgcgc aggcgggaat ctaggtttgt
    12061 cggcacagaa acttgtcggg aaaaacggtt tctttagatt ttacgttctg gattcccgcc
    12121 tgcg
//
LOCUS       DAWGJV010000053        12075 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWGJV010000053 DAWGJV010000000
VERSION     DAWGJV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.053"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="WHQ94_001049"
     CDS             complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="WHQ94_001049"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ2128343.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="WHQ94_001050"
     CDS             complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="WHQ94_001050"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ2128344.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="WHQ94_001051"
     CDS             complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="WHQ94_001051"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ2128345.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGNDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(2757..3257)
                     /locus_tag="WHQ94_001052"
     CDS             complement(2757..3257)
                     /locus_tag="WHQ94_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ2128346.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="WHQ94_001053"
     CDS             complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="WHQ94_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ2128347.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMFGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="WHQ94_001054"
     CDS             complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="WHQ94_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ2128348.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7148..8383)
                     /locus_tag="WHQ94_001055"
     CDS             complement(7148..8383)
                     /locus_tag="WHQ94_001055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ2128349.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8388..9185)
                     /locus_tag="WHQ94_001056"
     CDS             complement(8388..9185)
                     /locus_tag="WHQ94_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ2128350.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVIGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9188..9934)
                     /locus_tag="WHQ94_001057"
     CDS             complement(9188..9934)
                     /locus_tag="WHQ94_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ2128351.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9990..10547)
                     /gene="frr"
                     /locus_tag="WHQ94_001058"
     CDS             complement(9990..10547)
                     /gene="frr"
                     /locus_tag="WHQ94_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ2128352.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10727..11854)
                     /locus_tag="WHQ94_001059"
     CDS             complement(10727..11854)
                     /locus_tag="WHQ94_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ2128353.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11930..12005)
                     /locus_tag="WHQ94_001060"
     tRNA            complement(11930..12005)
                     /locus_tag="WHQ94_001060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11970..11972),aa:Thr,seq:cgt)
BASE COUNT         2268 a         3081 c         3767 g         2959 t
ORIGIN      
        1 tttaaaacac cacttttgta gtgtccagta atttcagcac agaatattca ataagacaat
       61 atgttctttt gaaaaatact tttggttttt tcgccgaaag caggtcggtt caagctgcgg
      121 aaattgtttg caattcttta aaagcagcgg cggaggtcac aatgaaatgt ccgaatgggg
      181 atgtggtcgg cggcagaaat catcaatgct gccgactgcc atacttctga aatctacaaa
      241 atgatgcatc gatcaaacaa tataccgctt taaaaaaacc gatgccgtct gaaacgcttt
      301 cggggtttca gacggcatca aaagggtacg gtcagcggat gatgccgcgc gccgattgtg
      361 cgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc ggcgcggcgg aggatgtctg
      421 ccttggcctc ttcaaacgga atgccgcgat ggtagagggt tttgtacacg tctttgacgg
      481 cggaaatctg ttctgcggta aaaccgttgc ggcgcatgcc ttcgctgttg agccccgccg
      541 gttcggcgcg gtagcccgat gccataaagt agggcggtac gtctttgtgt acgcctgcgg
      601 cgaaggcggt catggcgtag tcgccgatgt ggcagaattg gaaaaccagc gtgtagccgc
      661 ccaaaacgac gtagtcgccg atggtaacgt gtccggcaag cgaggcgttg ttggcgaaga
      721 tggtgtggtt gccgatgacg cagtcgtgcg cgaggtggca gtacgccata atccagttgt
      781 cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc taaattaaag gtggtgaatt
      841 cgcggatggt gttgccgttg ccgataatca gcttggtcgg ctcgtcgcgg tattttttgt
      901 cctgcgggat ttcgccgagg ctggcaaatt ggaaaatgcg gttgttttcg ccgatgctgg
      961 tgtggccgtt gatgacggcg tgcggaccga tttcggtatt cgcgccgatt tggacgttgg
     1021 ggccgataac ggtgtacgcg ccgactttga cgccggagtc gagttcggct ttggggtcga
     1081 tgacggcggt cgggtggatg agggtcatgt ttttcctttc ctgtcgtgtt gccgcgaaga
     1141 tgcgcgacgg caacaggttg tctgaaaact ttcagacgac ctttttctga acactcaaac
     1201 tacgcgtttg gcacacatga tgatggcttc gacggcaact tgcccgtcca ctttggcgac
     1261 ggcgttgaat ttgccgatgc cgcgccgact ggtcagcagc tcgacttcaa agacgagttg
     1321 gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct atgccggcga agaagaagaa
     1381 ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat
     1441 cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg ggaaaatggc cttggaactg
     1501 gggttcgttt atggtgacgt ttttaatcgc ggtcagggtt ttcatcggct cgaaggcggt
     1561 gatgcggtcg agctggagaa acggatagcg gtgggggatg agtttttgga tgtctttggc
     1621 ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt gaataaagtc ggtttggtta
     1681 tttgctgtct tgaccggcat ctgaaagctg ctgctccagt gttttgagcc gtttgttcat
     1741 ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat tctttatggg tggacatcgg
     1801 gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg acgtgccgcc
     1861 gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc cgccgccgat
     1921 gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg cgatgacggt
     1981 gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt tggtgccgtt
     2041 gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc cgatttctac
     2101 gtcgttgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat cgccggcaaa
     2161 agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga cgcgtctgcc
     2221 cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt cgcccagttt
     2281 gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga gcacggtgtt
     2341 tgcgccgatg tagacgttcg cgccgatttc gcagctggcg ggaacggtcg cgcccggttc
     2401 gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa acaggcgggc
     2461 gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc cttcaaatcc
     2521 gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga cttcggcttt
     2581 gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga gcgggcgcac
     2641 ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg tgatttggga
     2701 cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg aaagggtcag
     2761 cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt gacgtaaatc
     2821 acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat gacgcggttg
     2881 gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc ttcttcaaac
     2941 tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc ggcttgcgcc
     3001 ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccag accttcgcgt
     3061 tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt tttttgaatc
     3121 ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc gattttttgg
     3181 aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc ggcaaacgcg
     3241 cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc cgtctgaagg
     3301 gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg aagcgttgga
     3361 tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc gggcctaaag
     3421 gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta aaggtggatt
     3481 tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg gaactgctgt
     3541 tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg ctcaggcgga
     3601 cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag ctcggcggag acgttggctt
     3661 ttttgttgcc gccgtagctg attttttcgc cgtattcgtc atacactttc ggaccgagcg
     3721 tgccgctttc gtatccgcgc accgaaccca ggccgccgcc gtagaagttt tcaaagaagg
     3781 ggatttcttt ggttttgccg tagccgcccg caatgccgac ttcgccgccg aacatcagcg
     3841 tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag tagtattgca
     3901 gtttgctgcc gggcagggcg atttcagcgt tcacgcccgt caggtagccg cgcgtcggcc
     3961 ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac agccagcctt
     4021 tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt gataaagtcg gcatagtgtt
     4081 tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc caccaaaccg aaattcacgc
     4141 ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg gtggttttat
     4201 attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa acatcgtagc
     4261 ctaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag ccgttgagcg
     4321 tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac aggttgtctt
     4381 gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg ctcaagtcga
     4441 gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg ggtgtgccgg
     4501 caagcgggac ggcatcaaac tgtacgttgt cgaagtagcc caaaagctcg acgcgctctt
     4561 tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc aattcgcggc
     4621 gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg gatttcgttg acgtagattt
     4681 tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg tttggcagcg
     4741 gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc tgaatctcac
     4801 ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg gtcagcagtt
     4861 tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact ttgccccaac
     4921 ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg tcttcgttgg
     4981 tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg tagaagtcgg
     5041 ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt cgtgtcagcc
     5101 atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg tcggaataga
     5161 cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg tcaatcgtga
     5221 tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt tggatattga
     5281 gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg agtgtcgcct
     5341 gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta atggcgtcgt
     5401 tttgcagcat ttttgcgccg gtgatgttga gcgagccgat ggtggggcgt tcgataacgg
     5461 tcagcaggag ctgcccgtcc gcagtttcga cgcgtacgtc gtcaaagaaa ccggtggcgt
     5521 acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg actttgacgg
     5581 gcaggtagtt gaatacggta ctcggctcgg tacgctgcaa gccttcgacg cggatgtctt
     5641 ggatggtgaa gtcggcaagt gccaaaggcg atatgcccaa gaccatcagt gcggaagcaa
     5701 tctgtttcag tttcattgtc agttccttgt ggtgcggaat gcggtttcag acggcattcc
     5761 gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg aagaaggcga ccgccatcat
     5821 cagcatcatg agggcaagcc cgaagcgcaa accgatgttt tggacgcgtt cgcccaaagg
     5881 tttgccgcgt atccattcgg cagtataaaa cacgaggtgg ccgccgtcca aaacggggac
     5941 gggcagtagg ttcagcacgc cgaggctgat gctgaccagt gccaaaaatt ccaaataact
     6001 ttgcaagccg agttcggcgg actgtccggc aatgtcggca atggtcagcg gcccggaaat
     6061 atggctgacg gaggcgttgc cgctgattag tttgccgaaa aatttgaggg ttgtccacga
     6121 gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg acaacagacg gacggtagct
     6181 gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga cggaggccga cgcgcccgat
     6241 cagggtgtgg tcgggctgtt cgacagtatc ggggcggatg tcggcggtat gggtttgtcc
     6301 ggcgcgttcg tagttcaggg tgattttttt gccggggctt tggcgggtca ggtttgccca
     6361 ttcttgccat gaggtaatgg gtttgccgtc ggcggcagtc agcctgtcgc ccggtttcag
     6421 gcctgctttt tcggcggggc tgcctttttc cacgccaccg gcaacggttg tgattttaaa
     6481 gggcatcagt ccgatgtagc cttggttttt tgcgatttta ccggcttccg gcgtgcctgc
     6541 ggcatcgatg gtgcggacgg tttgcgcgcc tgatgccgtc tgaacgccga cggcgacttt
     6601 gccggcttcg aggttgagga cgatttcggt ttgcgcgctg ccccaatctg caacgggtgt
     6661 gccgttgacg gattgtattt tgtcgccgct ttggaagccg gcgcgggcgg caatggtgtc
     6721 gggttcgact gtgccgacat aggggcgcag ttcggttacg ccgaaggaaa agctcagtcc
     6781 gtacagcaaa accgccagtg cgagattggt cagcgggccg gcggcgacga tggcgatgcg
     6841 tttggcgggg tgttgtttgt caaaagcgta gggtaaatcg gcttctgata cttcgccttc
     6901 gcgcgtatcg accattttga cgtaaccgcc caacggaatc ggggcgaggc accattcggt
     6961 gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg acggaaaagc gtacgacttt
     7021 gacgccgcac aatctggcaa cgatgtagtg tccgaactcg tgcaggctga ccaaaatcag
     7081 gatggcgaag ataaaagcta gaagggtgtg caaatggttt tcctttgata acggtgttca
     7141 gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg tgcgcgtgtc cgggcatctt
     7201 gcgccaagag cccccctatg tcgcctatgc cgtctgaaaa gtcttgtgaa agacaatggg
     7261 cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc caaaaaggcg gcgacggcgg
     7321 cttcgttggc ggcgttcaat acgcagggcg cggctccgcc tgcgttcatg gcttcatagg
     7381 cgagcttcag gcaggggaag cggtcaaagt cgggcttttg gaaggtcagc gcggacaatg
     7441 cgtcgaaatc caggtcgccg acacccgaat cgatgcgctc gggcaaaccc aaacaataag
     7501 caatgggcgt acgcatatcg ggattgccca gttgcgccaa cacggagccg tcgcggtagc
     7561 gcaccatgct gtgtatcacg gattgcggat ggatgacgac ttcgagcttg tcaggcggac
     7621 agttgaacag ccaatgcgct tcaatcagct ccaaaccttt gttcatcatg gtggcggaat
     7681 cgacggagat tttgcgcccc atgctccaat tggggtgttt gaccgcttgg gcgggcgtaa
     7741 tgctgtcgaa cgtgtttaaa tcggcggtca gaaacgggcc gccggaagcg gtcaggataa
     7801 tcgaagcgat gccgtgttcg ttcagacggc cggcgtaatc gcgcggcaaa acttggaaaa
     7861 tggcgttgtg ttcgctgtcg acgggcagca ctgccgcgcc gtttgcacgg gcggtttcca
     7921 taaacaacgc gccggaaacc accagcgtct ctttgttcgc cagataaatg gttttgcctt
     7981 tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc gacgatggcg cacatgacac
     8041 cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg cgcgccgtgt aaaacctgcg
     8101 tcgccgtgcc gtcgcctttc aacaaagctt caagccgggc ggcgtgttcg gcatcggcaa
     8161 cgacggcata ttcggggcgg aacgtccggc attgcaccgc cagtttctcg acctgcttat
     8221 gccctgccag cgcgaatacg cggaattttt cggggtggcg ggagacaacg tccagcgtgc
     8281 tttcgcctat gctgccggta ctgcctaata tggtcaggac ttgtggtgtc ataatgggga
     8341 taactttata ccggatgccg tctgaagcgt tttcagacgg catagaatca atttaaaacc
     8401 gacatcatcg ccgcatagac gctgataacg gcaatcaggc tgtcggtacg gtcgaacacg
     8461 ccgccgtgtc cgggtagcag gttgctgctg tctttgatgc ctgccgcgcg cttgagccag
     8521 ctttccaaaa ggtcgccgca tacgctgaca acggtcaaca ccaaaccgat taacacggta
     8581 tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc gtacggcggt catgtacacg
     8641 gccacgcaaa ccgcgccgcc gattgcgcct tcccagcttt tgccagggct gattgccggt
     8701 gcgattttgt gtttaccgaa cgccttgccg ctgaaatacg cgcaaacgtc ggcaacccag
     8761 accagaccta tcacggcgag cagcggcagg gcatcatcgg gatgcgggcg cagggatacg
     8821 agcgcgaacc aaaacggcat gaccagaagc caaccgacgg cataaacctg ccaaccgccg
     8881 ttgagcctcc atttgaatct caaccataaa ggcatgatgg cgagccaaaa tgccaaaaca
     8941 acataccaaa ccaaattagg cagcatccag ccgcccgcat aggcaaccac gccgaaaacc
     9001 aaggttgcgg cgaggtaatg gttggtttta attttgcaca aaccgctcat acgggcatat
     9061 tcccacaagg caatcagggc aatcagtccg caaaatgcag cccacaacca ttgcggcgcg
     9121 taaaacagca tgcccagcat cagcggcagc agccacatag cagttatcac ccgttgtttc
     9181 agcatattca gttcctttgc tgttcgatag gcagttgctc ggaggtgcgt ccgaaccgcc
     9241 gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc cttgccgtca aaatcgggcc
     9301 acaaaatatc ggtgaaatac agttctgcat atgccatctg ccagagcagg aaattgctga
     9361 tgcgcgtttc gccgccggtg cggatgaaca aatccggttc cggtgcatcg cccagcatca
     9421 agtgtttcgc cagcgtgtct tccgtaatct cggatacgcc ttcggcaatc agtttgtttg
     9481 ccgcctgcaa aatatcccaa cgtccgccgt aatcggcggc aatgctcagg gtcaggccgg
     9541 tattgtttgc cgtcaaggct tccgcctctt cgatgccttg cagaatctgc cgattaaagc
     9601 gttcgcggct gcccagtatt tttaggcgca tattgttttc gtgcagacgg cgtacctgtt
     9661 tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac ttcgtcttcg gggcggcgcc
     9721 agttttcggt tgaaaaggca aacacggtca gatattgcac acccagtttg gcgcaatgct
     9781 tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc cattatgcgc gggaggaaac
     9841 gttttttcgc ccaacggccg ttgccgtcca taatcacggc gatatgcttg ggaatggcgg
     9901 tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc ctttcgcggt tcggcattca
     9961 aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca tcaaatcttc ttctttggca
    10021 gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca gtttttgaac cgcttcttcg
    10081 ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga ggagtttttt gatgtggtcg
    10141 ttggcatcgc ggcgcacgtt gcggatggag acgcggcctt cttccgcttc gccgcgtacg
    10201 actttaatca ggtctttgcg gcgttcttcg gtcagcatgg gcatcggcac gcggatcagg
    10261 tcgccgacag ctgccgggtt cagccccaag tttgaatcgc ggatggcttt ctcgactttg
    10321 accgccatat tgccctcaaa cggtttcacg ccgatggtgc gcgcgtccag aagcgttacg
    10381 ttggcaactt ggctgacggg gaccatgctg ccccagtatt cgacttccac ttggtcgagc
    10441 aggccggtat gcgcgcggcc ggtacgcact ttcgccagat tttctttcag tacttcgacc
    10501 gaacgctgca ttttgccttc ggctgttttt tgaatatcgt tgatcatatt gttctttcgg
    10561 tgggataagg tgggcgggag accgtctgaa cgcgtttcaa gccgttcaga cggcataaag
    10621 accgttaacc gcgaatagta ccgttattcg ggcataacga caaggtaggc ggattgggga
    10681 tgccgtctga agcgacaggc gtttcagacg gcatcgtgtc cgaccgtcag ccgtgttccc
    10741 gtgtttcaag caggctttgg cgcaggtgtt ggcgttcgtg ggcatccagc catttgcggc
    10801 gggtgcgttg cagcaggatg acgagggcgg aaatttcctg acgcatattg gtgctgagcc
    10861 agaggaagcc ctgccattgg tagtggaggt gttcggcgag ggcttccagt tcggggttga
    10921 tagcggtgtc gatgcggatg cggcgggcgt gtctgccgtt gataagggcg acggtttgtt
    10981 gcaggtcggt ttggagcagt gtgaagtggc ggtcgagcag ccggatttcg ctgccgttga
    11041 gtttgggaga ttgcagcttg gcggcagtgg tcaggagcag ctcggtggtg ttgacaattt
    11101 tacggtgggc gtgctgcatg gcttccatca tggcggggct gatgcggctt tcgcccgatg
    11161 tggcggcgag gtggctgcgg cttttgacca tgcgtgcgtt gatttggcgc attttcgcca
    11221 tgttctcttc gaggcgttcg cgggtcatgc gcctgccgtt gctgatttcg gcaatcattt
    11281 tgctgcagtc ggtcaggttg tcggcaagca tgaaacgcca catcagtgtg gatttcagcg
    11341 gcagcagttt ggcggcggcg atggcgatgg ccgcgccgat gaggacgttc atcgcgcgca
    11401 tcaggccgct gtcgaaccat tcgctgccgt tgtcgccgat gagcatgcac atcgtcagcc
    11461 ccgccagcat agggacgtag ccgtttttgc cgaccgccgc ccagccggcc agtgcgcttg
    11521 ccgtgccgac ggtgaggtag aagaggaggt tgccgtggaa ataatgctgg ttcagccata
    11581 aaacgcccaa acccgcgccc agcccgatga ccgtgccgag catacgttcc accgccttgg
    11641 agtaaatcgc cccttgaaac tggagcatgc cgaggacgac gaagacggtc atccctatcc
    11701 actcgccgtg ttggaggtgg agcagccggg cggaggcggt ggcgaacagg acggccccgc
    11761 cgagccggac ggcgtggatg aggcggcggt agcggtagcg ttcgtaggag ttgagccagc
    11821 ggctgacgag gcggttgcgt tgcgaggtgt tcatatcggt tgtgccgtct gaagcggaaa
    11881 tgtgaaaaag cacaggcttc ccgaggaagg gagggtctgt gcttggtatt ggtgccggag
    11941 aagggaatcg aacccccgac cttcgcgtta cgaatgcgct gctctaccga ctgagctaca
    12001 ccggcgtttt ttcgtcatga tatatatgaa cggttgtttg tgcaactttt cgggcgggcg
    12061 gcaaggcagt gcgcg
//
LOCUS       DAWGJV010000054        11878 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWGJV010000054 DAWGJV010000000
VERSION     DAWGJV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.054"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(403..1449)
                     /locus_tag="WHQ94_001061"
     CDS             complement(403..1449)
                     /locus_tag="WHQ94_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2128354.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAK
                     VAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKE
                     AQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRD
                     FADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKA
                     DSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFE
                     EAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVN
                     LLRDLHSGAYVDIR"
     gene            complement(1551..3959)
                     /locus_tag="WHQ94_001062"
     CDS             complement(1551..3959)
                     /locus_tag="WHQ94_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ2128355.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4029..5033
                     /locus_tag="WHQ94_001063"
     CDS             4029..5033
                     /locus_tag="WHQ94_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ2128356.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(5090..5788)
                     /locus_tag="WHQ94_001064"
     CDS             complement(5090..5788)
                     /locus_tag="WHQ94_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2128357.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            5815..6006
                     /locus_tag="WHQ94_001065"
     CDS             5815..6006
                     /locus_tag="WHQ94_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128358.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            6006..7544
                     /gene="murJ"
                     /locus_tag="WHQ94_001066"
     CDS             6006..7544
                     /gene="murJ"
                     /locus_tag="WHQ94_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ2128359.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            7676..8632
                     /locus_tag="WHQ94_001067"
     CDS             7676..8632
                     /locus_tag="WHQ94_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ2128360.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARTVPGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRTDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            8682..9464
                     /gene="trpC"
                     /locus_tag="WHQ94_001068"
     CDS             8682..9464
                     /gene="trpC"
                     /locus_tag="WHQ94_001068"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ2128361.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAENAPPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            9529..11829
                     /gene="recQ"
                     /locus_tag="WHQ94_001069"
     CDS             9529..11829
                     /gene="recQ"
                     /locus_tag="WHQ94_001069"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ2128362.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTADFSHNAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2405 a         3333 c         3406 g         2734 t
ORIGIN      
        1 aatccaaatt cttcggtaca gaaacttatc ggataaaacg gtttctttag attctacgtc
       61 ctagattccc gcctacgcgg gaatgacgat ggaaagattg ttgttgcttc ggataaattt
      121 ctgcagtttt aaataaccgg attcccgcct gctcgggaat gacggcatag gtttttttgt
      181 ttttccgata aattaccaca acccaaaatc ccgtcattcc cgcgacggcg ggaatccagt
      241 cctttaaact ccagccattc ccgataaatt cctgttactt ttcgtttcta gattcccgct
      301 ttcgcgggaa tgatgaatga cggtgtaaaa gtaactcgaa atccaaaaag ccataccgcc
      361 cggatttttg ccgatcggta tggctttttg cttcaaaccg ccttagcgga tgtcgacata
      421 cgcgccggaa tgcaggtcac gcaacaggtt gacggttgcc tgttcggctt tttgttggaa
      481 gatgtattgc cgcacggaat tgcggatacg ttcctgaggt gtgccggcat cgcgcacttc
      541 gttcaatttg atgatatgcc agccgaattg ggtgcggacg ggcgcgccga cctgtccggg
      601 tttgagcgcg tggacggctt cttcaaaggc gggaaccatc acgccgtcgg caaaccagcc
      661 caaatctccg ccgttgcccg cgctcgcgtc ttgcgaatat tggcgcgcta ggctggaaaa
      721 gtctgtgccg ctgcgggcct ctccgtagat tttgcggatg gtgctttccg cgccgacggc
      781 ggcgttttcg ctgtcggctt taatcaggat gtgttgggcg cggtattggc gcaacggtgc
      841 gccttcgggc agggtgatgc cttgtttttg cgcctgctcg aggaaggcat cgatttcagc
      901 ttcgctcacg cggctgttct gcatcactgc ctgctggcgg actttttcgg caatgatgtt
      961 gtcggcaaaa tcgcggcgtt gggcggggct gaggtttttg agggcggaat tttttgcgac
     1021 gacggcatcg atttccgctt cgcttgcttg aatgttgcgg cgtttgcccg cctgtacaat
     1081 cagggattgg ttgacaagct gcatcagcac ctgtcgggac agctcggatt cgcttatctg
     1141 cgcctctttg ggcaggttgg ctttggcttc ggcaacggct tcggcaagcc ggcggcgcgt
     1201 gatgacttcg ttgtcggcaa cggcggcaat gccgtctgaa aagcggatac cgccctgctg
     1261 ttgtgcgggt gcggcttctt tttgcgccgt ggcggcaact ttggcaactt tggcaacttt
     1321 ggcaactttg gcaactttgg caactttggc aactttggcg gatgcggttt ttgccttttg
     1381 cggtgcggcg tggacatcgg ctgctgccag caatgcggcg gcaatcatca gggctttgat
     1441 tttcatcatt ctttccttat cgtttgaatc ctacctttga tggcgggttt ggggttgtcg
     1501 gtggaatgcc gtcttgtgcg ggaacggtgc tcctgccggg tttccgacgg tcagggtcgt
     1561 ttgttacgtc cggcggaaag agagtgggcg gggatgtagc cgggaacggc gacatccatc
     1621 ctgtctgcgg ggtttctgcc gacactgctg aggtctttca actgaagtga gaaaaagaca
     1681 gcgtttttgt aggtgttttc gccggtaacg tagcgttggg cgtacacgcc cgcgccccag
     1741 cagccgcaac tgcttttgta ttccgcaccc gccagcacct ctatcggttt tttggcttca
     1801 aaaccgtagt tgtaacggac gacggccgac aggttgcgcg tcagcggcca ttgtgcggac
     1861 aggtcgagct ggctgagttt gtcgtaaaaa taggaaccgt cggacttcag gtagattttt
     1921 tcgttgcgcc cgtatttgta gcgggcgttc agcactttgc cctgtgcggg acggtagctt
     1981 gcaccgacgg cgtagttctc ggcgcgtttg tcgttttggt tgtagtggat gctgctgtcg
     2041 aggatgaagc ggctgccgat gctgccggag gcaaatgcca cccagtcgga acggttgcgc
     2101 ggttttttgc cgacgctgcc gtcaagcatc accgcatcat ccttgaaata gaatttctga
     2161 ccgatgccgg cgcggaaacg ctcttccccc gtcgcgccgt ccaaaatacg gctttgcacg
     2221 gcggcggaaa ggctgtttgc ggtgttaatc ctgtcgttgc cgtaatagag gttttcgcga
     2281 aagagctgcc cgtagccgaa gctgctttcc gacgaatcga aattgggcag gtcgttttgg
     2341 gatttggcag gaatatagtt gtagaacagg cgcggctcga gggtttgcag gactccgccg
     2401 ccgaacatcc gcgtattccg ctcaaaagtt gcgccgctgt cgatgttgac aatgggcaga
     2461 gtgcggctga cgcgtcgggc ttcttggctg ccgaagcggt tgaggctgta ataggtggcg
     2521 tgcagtccga gtttgggacg gacgtaaccc cagctgttgc tgaaatccca tttgatgtcg
     2581 ggatagacga cgaggcggct gccgtcttgg cggctgtcgt ggctgaagcg ggtaaattgt
     2641 gcggacacgc cgatttgcgc cctgccggtg tttttgcgcc aatcggcgga aaggcgcggc
     2701 atcagggcat acggtttgtc tttgtagccg ctttggtttg ccagcgtctg gtatttcaga
     2761 accgaaaggc cggcattcag gctgccgccc gccgccctgc cgccataatc cagccataca
     2821 cggcggttga ggttgacgtt gccggcgatt tctttgttgc cgtaaaagtc gcggtagtag
     2881 ccgctgtcgg agacttggtt gaaatcgaca cccgcctgaa gcgtgtcgga aatgtcgtgc
     2941 cgatgctgcc atttcgcctg atagcggtta ttcctgccgc ttttcttgtc gtgcggcagc
     3001 caggtcaggt cggactggcc ggcataatcc ggccgcaggt agcgtacctg cccgtcaaag
     3061 accgcgccgc gttcgccgat cacgctgggc gcgaacgtgg catcgagatt gggggcaagg
     3121 ttgaaataat agggaacgga aagggaaacg ccgtccgaac cggcggacag tgagggaaca
     3181 agcaggccgc ttttgcggtt gccgtcaagc gggaagtccg cccaaggggt gtagaaaatg
     3241 ggaacgccgc cgaacacgaa ggcggcgtgt ttggcaacgc ctatgccttt ttcccgatcg
     3301 gcttcgacgg aagcggcctt gacataccag ccggcatcgc cggcggaaca ggtgttgaat
     3361 tgggtttccg tcagtttgta atgcccttcg cccaacattt cggcggtgcg gctgacgctt
     3421 tgcagccgcc gtccgccgtg ttcggtttcc atacggacgt tgtgcgcttc cccggtctgc
     3481 tgctcgagat tgtaggtcag ggtttcgccc cgaatcagcg taccgtcctg ttggagggcg
     3541 aaccggtcgc ctgcggtaac ggtgtcgccc gactggtcgt aatccgccca atcggtattg
     3601 agggtcgtcc ggttgcgttc gacgacgacg ttgccttcgg cacgcacctg cacctgcgac
     3661 tgtccttcca ccttgtcggc aacaatgcgc gtatagtctt cggggatgga tgcttcgccg
     3721 ctgcctcgga cggcggcttc ggttctctcg gggctgccgc tttcgttact gcaaaacagg
     3781 caggtcgaac cgaggctcag gctggtaggc tgtatgggtt cggacacgct ccgaacgctt
     3841 cctccggcgg tcggattgtc cgtttcctcc gccgcaacgg catcggcggc ggcgcaatgc
     3901 gtgccgaagc agaggcccaa tgccagcacc agtggtttga gtgaaaataa acgagccaaa
     3961 atcgcccctc aagtcggttt gccagttaga atagcgttta ttgtaacctg aaatgcttta
     4021 gtactgttat gcaacgacaa atcaaactga aaaattggct tcagaccgtt tatcccgaac
     4081 gggacttcga tctgactttt gcggcggcgg atgctgattt ccgccgctat ttccgtgcaa
     4141 cgttttcaga cggcagcagt gtcgtctgca tggatgcacc gcccgacaag atgagtgtcg
     4201 caccttattt gaaagtgcag aaactgtttg acatggtcaa tgtaccgcag gtattgcacg
     4261 cggacacgga tttggggttt gtggtattga acgacttggg caatacgacg tttttgaccg
     4321 cgatgcttca ggagcagggc gaaacggcgc acaaagccct gcttttggag gcaatcggcg
     4381 agttggtcga attgcagaag gcgagccgtg aaggggtttt gcccgaatat gaccgtgaaa
     4441 cgatgttgcg cgaaatcaac ctgttcccgg aatggtttgt cgcaaaagaa ttggggcgcg
     4501 aattaacatt caaacaacgc caactttggc agcaaaccgt cgatacgctg ctgccgcccc
     4561 tgttggcgca gcccaaagtc tatgtgcacc gcgactttat cgtccgcaac ctgatgctga
     4621 cgcgcggcag gccgggcgtt ttagacttcc aagacgcgct ttacggcccg atttcctacg
     4681 atttggtgtc gctgttgcgc gatgccttta tcgaatggga agaagaattt gtcttggact
     4741 tggttatccg ctactgggaa aaggcgcggg ctgccggcct gcccgtcccc gaagcgtttg
     4801 acgagtttta ccgctggttc gaatggatgg gcgtgcagcg gcacttgaag gttgcaggca
     4861 tcttcgcacg cctgtactac cgcgacggca aagacaaata ccgtcccgaa atcccgcgtt
     4921 tcctcaatta cctgcgccgc gtatcgcgcc gttatgccga actcgccccg ctctacgcgc
     4981 tcttggtcga actggtcggc gatgaagaac tggaaacggg ctttacgttt taaacccaat
     5041 caaaatgccg tctgaaaacc aagtttcaga cggcattttt caaacgggct tactgcgcgg
     5101 ctttttgttc ttcgcgtact ttgtccgcca aaaggtcgat ggtgttcata ccggactccc
     5161 agtcggcaaa ttcaacttta tatttaccgc cgacgataac cgtgggcgta ccgtcgattt
     5221 ggaaggtttc ggtcagctcc tgcattttgt cggcgcgcgc ctggctttcg ggggactcgt
     5281 aggcggcaag gacttttttg ccgtcaaagg cggtttgttc gcccagccat tttttgagga
     5341 cttccggctc ttgcagcttg attttttggt tgaccatcgc atcgaaaata tggctgttcg
     5401 ccacatcttt gctgtcggcg gcagccatat cgacggcggc ggcgaggcgt gccagcggca
     5461 gcatttcttt ttgccaaacg acatgctcgg tacgcaggta catatcgtct ttaaaagact
     5521 tggcgtgttt gctcaaaaca ggttcgaggt gggcgcagtg cggacagaaa tagccgaaaa
     5581 actcaaggac ttcgactttg cctgcctgct gttggggaat cgggttggca aggacggtat
     5641 agttttgccc ttcgaccagc cctgccgggg cggcggctgc cgaagcggca ggcgcgctgt
     5701 cggcggggac gctggtttgg actttgctgt cgcacgcggc aagggcgaac agggcggcaa
     5761 cgccgagggc gagttgtctg gatttcatac ggctctccgt gatgttggaa acagatgttg
     5821 gaaatcaatc ggaatccggc tattttattg catttccgcg tgttgataca gtttgccgcc
     5881 ggaaaaggac gttttcgttt cgggaaaccg cttcagacgg catcaaaccc gatgccgtct
     5941 gaagcggttt ctgtcgtaca atacgcgccg ttgccccaaa cgggtacgac tgttgaggaa
     6001 caatgatgaa tatgctggga gctttggcaa aagtcggcag cctgacgatg gtgtcgcgcg
     6061 ttttgggatt tgtgcgcgat acggtcattg cgcgcgcatt cggcgcgggc atggcgacgg
     6121 atgcgttttt tgtcgcgttc aaactgccca acctgcttcg ccgcgtgttt gcggaggggg
     6181 cgtttgccca agcgtttgtg ccgattttgg cggaatataa ggaaacgcgt tcgaaagagg
     6241 cgacggaggc ttttatccgc catgtggcgg ggatgctgtc gtttgtgctg gtcatcgtta
     6301 ccgcgctggg catacttgcc gcgccttggg tgatttatgt ttccgcaccc ggttttgcca
     6361 aagatgccga caaatttcag ctctccatcg atttgctgcg gattacgttc ccctatatct
     6421 tattgatttc actttcctct tttgtcggct cggtactcaa ttcctatcat aaattcagca
     6481 ttccggcgtt tacgcccacg ttcctgaacg tgtcgtttat cgtattcgcg ctgtttttcg
     6541 tgccgtattt cgatcctccc gttaccgcgc tggcttgggc ggtttttgtc ggcggcattt
     6601 tacaactcgg cttccaactg ccctggctgg cgaaactggg ttttttgaaa ctgcccaaac
     6661 tgaatttcaa agatgcggcg gtcaaccgcg tcatgaaaca gatggcgcct gcgattttgg
     6721 gcgtgagcgt ggcgcagatt tctttggtga tcaacacgat tttcgcgtct tatctgcaat
     6781 cgggcagcgt ttcatggatg tattacgccg accgcatgat ggagctgccc ggcggcgtgc
     6841 tgggggcggc actcggtacg attttgctgc cgactttgtc caaacactcg gcaaaccaag
     6901 atacggaaca gttttccgcc ctgctcgact ggggtttgcg cctgtgcatg ctgctgacgc
     6961 tgccggcggc ggtcggactg gcggtgttgt cgttcccgct ggtggcgacg ctgtttatgt
     7021 accgcgaatt tacgctgttt gacgcgcaga tgacgcaaca cgcgctgatt gcctattctt
     7081 tcggtttaat cggcttaatc atgattaaag tgttggcacc cggcttctat gcgcggcaaa
     7141 acatcaaaac gcccgtcaaa atcgccatct tcacgctcat ctgcacgcag ttgatgaacc
     7201 ttgcctttat cggcccactg aaacacgtcg gactttcgct tgccatcggt ctgggcgcgt
     7261 gtatcaatgc cggattgttg ttttacctgt tgcgcagaca cggtatttac caacctggca
     7321 agggttgggc ggcgttcttg gcgaaaatgc tgctctcgct cgccgtgatg ggcggcggac
     7381 tgtgggcggc gcaggcttgc ctgccgtttg aatgggtacg cgccggcgga atgcggaaag
     7441 cggggcagct ctgcatcctg attgccgtcg gcggcggact gtatttcgca tcactggcgg
     7501 ctttgggctt ccgtccgcgc catttcaaac gcgtggaaaa ctgaccgatg ccgatatcct
     7561 ttttcggcaa acgcttttcc acgcctatat tcgggcgcgc atcccccacg tccgcaccag
     7621 ccggacgggc tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat accttatgat
     7681 tttaacaccg ccggacacgc cctttttcct ccgcaacggc aatgccgaca cgattgccgc
     7741 caaattcctg caacgccccg cacccacata ccgccgcgag ctgcttcccg acagcacggg
     7801 taaaaccaaa gtcgcctacg atttttcaga cggcatttcg cccgatgcgc cgctggtcgt
     7861 gctgtttcac ggtttggaag gaagcagccg cagccattac gcggtcgaac tgatgctcgc
     7921 ggtacgcagc cgcggttggc acggcgcagt cgtccatttc cgcagctgcg gcggcattgc
     7981 caacaccgct ccggtgttct accacttggg cgataccgcc gaaatcgcct ttactttgga
     8041 cacgcttgcc gcgcgttacc gtgaaatata cgccgtcggc gtatcactgg gcggcaacgc
     8101 gctggcaaaa tatttgggcg aacagggcga aaacgcgctg ccgcaagccg ctgccgtcat
     8161 ctccgccccc gtcgatgcag aggcggcagg cagacgcttc gacagcggca tcacgcggct
     8221 gctctacacg cgctacttcc tccgcaccct gatacccaaa gcacggacag ttccgggttt
     8281 tcagacggca tttgccgcag ggtgcaaaac actgggcgag tttgacgacc gtttcaccgc
     8341 accgctgcac ggctttgccg accggcacga ctactaccgc cgaacttcct gcaaaccgct
     8401 gctcaaacac gttgccaaac cgctgctcct gctcaacgcc gccaacgacc ccttcctgcc
     8461 gcccgaagcc ctgccccgca cagacgaagt gtccgaagcc gttaccctgt tccagccggc
     8521 acacggtggt catgtcggct ttgtcggcag caccggtggc aggctgcacc tgcaatggct
     8581 gccgcagacc gtcctgtcct atttcgacag cttccgcaca aacagtcgtt aacggtttga
     8641 tgctaatatt ccccctttcc cagacgaata cggagcccga catgaccgac atcctcaata
     8701 aaatccttgc caccaaagca caggaagttg ccgcccaaaa agccgccgtc aacgccgaac
     8761 acatccgcgc acttgccgaa aacgcaccgc ccgtccgcag cttcatcgat tcgatacgcg
     8821 gcaaacaccg tctaaacctg cccgctgtca ttgccgaaat caaaaaggca agcccgagca
     8881 aaggcctgat ccgtccggac ttccgaccgg ccgagattgc acgcgcctat gaaaacgccg
     8941 gagcggcgtg tttgtccgta ctgaccgacg aaccctattt ccaaggttcg cccgaatacc
     9001 tcaaacaagc gcgcgaagcc gtatcgctgc ccgtgctgcg caaagacttc atcatcgacg
     9061 aataccaggt ttatcaggcg cgcgcatggg gggcggatgc cgtcctgctg attgccgcag
     9121 cactggaaca ggaacaattg gaacgctttg aagcggtggc gcacgaattg ggcatgaccg
     9181 tcctgctcga gctgcacgat gaaaccgaat tggaaaaatg ccgcaatctg accacgccgt
     9241 tgtggggcgt aaacaaccgc aacctgcgga cttttgaagt ctccctcgac caaaccctgt
     9301 cgctgctgcc cgcgctggaa ggcaaaaccg tcgttaccga aagcggcatt acaggtaagg
     9361 cggatgtgga atttatgcgg gcgcgcggcg tgcacacctt cctgatcggc gaaacgttca
     9421 tgcgtgccga cgatattgaa gcagaagtgg gcaaactctt ctaaatctcg atttcagacg
     9481 gcatattgcc gccgaccgat caacatcctt accgagcacg gaaccatcat gcaccgaccg
     9541 accgccaaac agattttgca cgaagtattc ggttatcccg aatttcgcgg caggcaggag
     9601 gctgtcatca atactttggc aggcggcggg agtttgacgg tgctgatgcc gacgggcggc
     9661 ggcaagtctt tgtgttacca gattcccgcg ctgatgcgcg aaggcgtggc ggttgtcgta
     9721 tcgccgctga ttgcgttgat gaacgaccaa gtggcaaacc tgcacgccgc cggcatcgaa
     9781 gcggcggcag tcaacagcgg cacatcggca gatgaggcgc gcgagattgc cgacaagctt
     9841 gcccaaggcc gtctgaaact gctttatgtc gcaccggaac gcttggttac cgaccgcttt
     9901 ttgcgttttc tcgaccaaca aaccgtcagt ctgtttgcca ttgacgaggc gcattgtgtc
     9961 agccagtggg ggcacgattt ccgccccgaa taccaacagc tcggcatcct tgccgaacgc
    10021 tatccgaacg tcccgcgcat cgccctgacc gctaccgccg atgccgccac gcgcgccgac
    10081 atcaagcatt atctgcactt ggacaatgcg cccgaatttg tctccagctt cgaccgtccg
    10141 aatatttatt atcaggttat cgaaaaaaac aacggcaaaa aacaattgct ggatttcatc
    10201 cgcaaagaaa tgacggggca aagcggcatt gtgtattgcc taagccgcaa aaaggttgaa
    10261 gatgtggcgc agtttttgcg tgaaaacgga ttaaacgcga ttccgtatca tgctggtttg
    10321 agcatggacg tgcgcgagga aaaccaacgc cgctttacgc atgaagacaa tattatcgtg
    10381 gtggcgaccg tggcgttcgg catgggcata gacaaacccg acgtgcgctt tgtcgcccat
    10441 ctcgatatgc cccagagtgt cgaacatttc tatcaggaat cggggcgtgc cggccgggac
    10501 gggctgcctg ccgcgagctg gctgtgttac ggtttgaacg attgggtgtt gctgcgcgaa
    10561 cggattgccg aaggcaacag cgacgaggtg caaaagcaaa tcgaaatgca aaaactcgat
    10621 gccatgcttt ccgtctgcga aaccgccgcc tgccgccgcg tactgctgct caaacatttc
    10681 ggcgaagaat ccgaaccctg cggccattgc gacaactgcc tgcatccgcc cgtacggttt
    10741 gacggcacgg tgttggtgca aaaattactc agctgcgtgt accgcgccgg acaacgtttt
    10801 gccgccggtt acatcaccaa ccttttgcgc ggcaaaagcg acgattggat acgcggcaac
    10861 cggcacgaac aactgtccac cttcggcatc ggtacggagt tgtccgacaa agaatggcgc
    10921 agcgtcatcc gccagtgtat cagcctcggc tacctcaccg tcaacattac ccgatatcag
    10981 gcattgcaac tgaccgaagc cgccaaaaaa gtcctcaaag gcgaaaccga agtgatgctg
    11041 cgtccgctca agcgcgacaa gcccgccgcc cgcaccctca aagacaactg gctgcgtacc
    11101 gaacgcgaag aacgcctgtg gcaggcattg cgcgtttggc gtatgaaaca ggcagaagcc
    11161 gaagtcatcc ccgcctatat gattttcggc gacaaaaccc tgcgcgacct tgtcgaaaaa
    11221 atgccgcaag acctcaacgg actgcacgac atctacggtt tgggcgaagc caaaatcgac
    11281 cgtttcggac acggcatcct cgaagtctgc cgcaacaccg ccgactttag ccacaatgcc
    11341 gtcatccgcc cgcaaaccga acgcgaacaa caactgcgtc aaaaactcga ggcctggcgg
    11401 tatgaacagg caagggcgga aaactgcgcc ctgcatgccg tcctctccga cgaaagcctt
    11461 gccgatatgc ttgccgatac gcccgaaacc gaaaccgacc tcgaaggcgt acacggcttg
    11521 ggcagcgtac gcgccgccaa atacggacgg gacatcctcg ccgtctgccg tccgttttca
    11581 gacggcatcg atgaaaccgc caaacacaaa cgccgcctga tgcgcgccct gatccaatgg
    11641 tgcaacgaaa cggcaaaaca cgaacagtcc gaaccctacc gcattctcag caaagccgcc
    11701 ctacgcgcca ttgccgccaa acagccggaa ggtttggcgg agcttgccgc cgtatatggc
    11761 gtaggcgaag aaaaagccgc acgttacggt gcggcggtgt tggcggtatt ggaacgaaat
    11821 gccgtctgaa aaccgttacc aagtttcaga cggcattgcc tctatttaaa aattcctg
//
LOCUS       DAWGJV010000055        11772 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWGJV010000055 DAWGJV010000000
VERSION     DAWGJV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.055"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            98..814
                     /locus_tag="WHQ94_001070"
     CDS             98..814
                     /locus_tag="WHQ94_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ2128363.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WHQ94_001071"
     CDS             963..1865
                     /locus_tag="WHQ94_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ2128364.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGLSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1908..2681
                     /locus_tag="WHQ94_001072"
     CDS             1908..2681
                     /locus_tag="WHQ94_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ2128365.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPASRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WHQ94_001073"
     CDS             2793..4481
                     /locus_tag="WHQ94_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ2128366.1"
                     /translation="MLNKDQFSDNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WHQ94_001074"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WHQ94_001074"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ2128367.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDRRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5842..6225
                     /locus_tag="WHQ94_001075"
     CDS             5842..6225
                     /locus_tag="WHQ94_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ2128368.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6340..6813
                     /locus_tag="WHQ94_001076"
     CDS             6340..6813
                     /locus_tag="WHQ94_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ2128369.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6939..8042
                     /gene="mnmA"
                     /locus_tag="WHQ94_001077"
     CDS             6939..8042
                     /gene="mnmA"
                     /locus_tag="WHQ94_001077"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ2128370.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8113..9783
                     /locus_tag="WHQ94_001078"
     CDS             8113..9783
                     /locus_tag="WHQ94_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ2128371.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLHHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9895..11529
                     /locus_tag="WHQ94_001079"
     CDS             9895..11529
                     /locus_tag="WHQ94_001079"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ2128372.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRSNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHNQGIDSIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVVGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2916 a         3188 c         3106 g         2562 t
ORIGIN      
        1 aatttgtgca atgctttaat ttccccgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaagaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ctggcaaaaa
      241 gctgaccgaa tccgaacttt gccgccagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcagggga gaccgcgttg gctggaaccg
      541 cctgtccaat gcctttcacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc acatatcatg cggcaataaa ggaggtcagg
      961 agatggacag cctgatgaca ttgatttcgg ttttgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcgggacg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggtgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cctatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg gcagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat attgccgccc cgtttctgat ggtggtgttt tccacgctgg
     1861 gctgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggagggcgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 aggggctgcc tgccagccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aagccgtggc atcggagctg gtcaggcggc gcggcagcct gcggattatt accaacaata
     2281 tccacgtcgc ctccatcgtt tcggcgcgga cggattacac ggtcattatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa ttttcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaaaccatcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaatttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaagaaatgg gcgatcgcga agtacctgtt tcacaaacca
     3961 tttacatcga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggttggc ggctgtttca
     4681 taccttgagc ggggaattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaagtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac attatcatca aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttggacagac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttctctca gccggcaggg atgcggttaa tgccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     5701 tgcagacagt acaaatagta cggaaccgat tcactcggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg gggcaacgcc gtacaggttt ttgttaatcc actatattga
     5821 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
     5881 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
     5941 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
     6001 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
     6061 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
     6121 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
     6181 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
     6241 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
     6301 taagcacatt ccccgtctga tattataagg agcagaaaga tgaaaaaatt attggcggcc
     6361 gtgatgatgg caggtttggc aggcgcggtt tccgccgccg gagtccacgt tgaggacggc
     6421 tgggcgcgca ccaccgtcga aggtatgaaa ataggcggcg cgttcatgaa aatccacaac
     6481 gacgaagcca aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa
     6541 gtgcacaccc acatcaacga caacggcgtg atgcggatgc gcgaagtcaa aggcggcgtg
     6601 cctttggagg cgaaatccgt taccgaactc aagcccggca gctatcacgt gatgtttatg
     6661 ggtttgaaaa aacaattaaa agagggcgac aagattcccg ttaccctgaa atttaaaaac
     6721 gccaaagcgc aaaccgtcca actggaagtc aaaatcgcgc cgatgtcggc aatggaccac
     6781 ggtcatcacc acggcgaagc gcatcagcac taatctgctg aaaatatttg aaatgccgtc
     6841 tgaaaaagcc cgggctttca gacggcattt ttatgaccgc ctttaaaatg tgttaaaatc
     6901 tgcctttaaa aaccgccgtt tccaaccata ttctacgcat gaatacgaca gcaaaccctt
     6961 ccaatatcat cgtcgggctc tccggcggtg tcgattcttc cgtaaccgcc gccctgctca
     7021 agcagcaggg ttatcaagtg cgcggtgtgt tcatgcagaa ctgggaagac gacgacaacg
     7081 acgaatattg cagcatcaaa caggattcgt tcgatgccat cgccgttgcc gatattatcg
     7141 gcatcgacat cgacatcgtt aatttcgccg cgcaatataa agacaaagtc tttgcttatt
     7201 ttcttcagga atacagtgcg gggcgcacgc cgaatcccga tgtgttgtgc aacgccgaaa
     7261 tcaaattcaa atgctttttg gattacgccg tagggcaggg cgcggatacc atcgccaccg
     7321 gacactatgc gcgcaaagaa gtccgcaacg gcgtgcatta cctgctcaaa ggtttggatc
     7381 gaaacaaaga ccaaagctat ttcctctacc gcctcaagcc tttccaactc gagcgcgcga
     7441 tttttccgtt gggcggtttg gaaaaacctg aagtccgccg ccttgccgcc gaatttaatt
     7501 tgccgaccgc cgccaaaaaa gacagcacgg gcatctgctt catcggcgaa cgcccgtttc
     7561 gcgagtttct gcaaaaatac ctgccgaccg acaacggtaa aatggttacg cccgaaggga
     7621 aaaccgtcgg cgaacacgtc gggctgatgt tctacacgct cggacagcgc aaaggtttgg
     7681 gcatcggcgg cgcgggcgaa ccgtggtttg ttgcggctaa agatttgacg aaaaacgaac
     7741 tgattgtcgt gcaaggacac gaccatccgc tgctctatac ccgcagcctt gtgatgaacg
     7801 atttgagttt cacgcttccc gaacgcccga aggcaggacg ctatacctgc aaaacgcgtt
     7861 accgtatggc ggacgcgcct tgtgagttgc gctatttgga tgaagaaacc gccgagctgg
     7921 tgtttgacga accgcaatgg gcggttacgc cgggtcagtc cgccgtgctg tacgacggcg
     7981 acatctgttt gggcggcggc atcatccaaa cgaccgacaa gcctgtcatc atcacacgat
     8041 aaaacttatg ccgtctgaaa cagttttcag acggcattgt tccgctcaat tccactttaa
     8101 agaccgatac ctatgaaaaa aatttggtta gacagctacg agaagggcgt cagtgccgaa
     8161 atcgacatca cgcaatacaa ttccatcagc gacgtattcc gccaaagcgt ggaaaaattt
     8221 gcccgtctgc ccgcttttca aaatatgggc aaaacgctga cttatgccga aaccggcaaa
     8281 ctggcgactg atttcgcctc ttatctgcaa aacgtcctca agctgccgcg cggcgaacgt
     8341 gttgccatta tgatgccgaa cgtattgcaa tacccgatag cccttttcgg cattttgcag
     8401 gcaggtttgg tggcggtgaa caccaatccg ctctatacgc cgcgcgagct ggagcatcag
     8461 ctgaaagaca gcggcgcgac cgccatcatc gttttggaaa atttcgccaa cacgctggag
     8521 ctggtgctgc cgcgcacgca gatcaaacac gtcatcgtcg cctccgtcgg cgaaatgttc
     8581 gggctgctta aaggttcgct gatcaatttc atcatccgga aaatcaagaa aatggtgccc
     8641 gaataccgta ttcgggaaac cgtttccttt cagacggcat tgaaagaggg agcgaagcac
     8701 gttttccaac ctgtcgcatt aaaccgcgaa gataccgcgc tgctgcaata cacgggcggc
     8761 acgacaggtg ttgccaaagg ggcggtgctg agtcacggca acatctgcgc caatatgctt
     8821 caggcaaaag aatggattaa aaaccagttg cgtgagggaa aagaaaccgt tatcgccgcc
     8881 ttgccgctgc accatatttt tgccttaacc gtgaatctga tgatttttgc caatgccggc
     8941 tcgaaaatca tcctgattac caacccgcgc gatatgaaag gctttatcgg acaactgaaa
     9001 aaacagcggg ttaatgtatg tatcggcgtg aacacactgt ttaacgcaat ggttaaccgt
     9061 cctgattttg ccgaagtcga tttttcagga ttgcggctga ctttgggcgg cggtatggcg
     9121 acccaaaaag ccgttgccga aaaatggaaa aaaatcaccg gcacgcccat cgtcgaagcc
     9181 tacggtctga ccgaagccag ccccggcgtg tgctgcaacc ccttaaacat cgaatcatac
     9241 agcggcagca tcggtttgcc cgtctcgtcc accgaagtcg aactgcgcga cgcaaacggc
     9301 aaagaagtcc ccgtcgggca gccgggcgaa ttgtgggtaa aaggcccgca agtcatgaaa
     9361 ggctattgga accgtcccga agaaaccgct aaagccatag acgcgcgcgg tttcttggaa
     9421 accggcgata ttgccgtcat ggacgaaaaa ggctggttga agctggtcga tcgtaagaaa
     9481 gacctcgtcg ttgtttccgg attcaatgtt tatccgaacg aaatcgagga agtcatcgcg
     9541 catcacggca aagtcatgga agtcgcctgt atcggcgttc ccgacgaaaa aaccggcgag
     9601 gcactcaaag tattcgtcgt caaaaaagac ccgtcattga ccaaagaaga acttatcgcc
     9661 ttttgccgca ccgaattaac cgcatataaa gtaccgaaaa atatcgaatt ccgcgacgag
     9721 ttgcccaagt ccaacgtcgg caaaatcctg cgccgcgagt tgcggcaaag taccgggaaa
     9781 taaagaaaag atgccgtctg aaaacagccg tccaccgttc agacggcatc cgtccgtttg
     9841 ccagaaccgc gcgcttcacg ttaaaatcac gcattccaac atgggtattc catcatgacc
     9901 aaattcattt tcgtaaccgg cggcgttgtc tcctcactgg gtaaaggtat cgccgccgct
     9961 tctattgccg ccatcctcga atcgcgcggc ttgaacgtta ccatgctcaa gctcgatcct
    10021 tatatcaacg tcgatcccgg cacgatgagc ccgttccaac acggcgaagt gttcgtaacc
    10081 gacgacggcg cggaaaccga cctcgacttg ggacactacg aacggtttat cgattccacg
    10141 atgacccgcc gcaacagctt cagcacgggg caggtgtacg aaaacgtcat cgccaaagaa
    10201 cgacggggcg actacctcgg cggcacggtt caagtcattc cgcacattac cgacgaaatc
    10261 aaacgccgca tccacgaagg cgcggcaggt tatgatgtgg cgattgtcga aatcggcggc
    10321 acggtcggcg acatcgaatc gctgccgttt ttggaagcca tccgccaaat gcgaagccag
    10381 ttgggacgca gcaacaccct gttcgcccac ttgagctacg tcccctacat cgccgccgcc
    10441 ggcgaaatca aaaccaaacc gacccagcat actgttaaag aaatgttgag catcggtttg
    10501 caaccggaca tcctgatctg ccgtatggac agagaaatgc ctgcggacga gcgccgtaag
    10561 attgcattgt tctgtaacgt tgaagaacgt gccatcgtgg gcagttacga cgtggacagc
    10621 atttacgaat gccctgaaat gctgcacaac caaggcatcg acagcattat taccgagcag
    10681 ttgcagctta atgtgcagca ggcggatttg accgcgtgga aaaaaatcgt ccacgccatc
    10741 caaaacccga aacacaccgt caaaatcgcc atggtcggca aatacgtcga tttgaccgag
    10801 tcctacaaat cgctgatcga agccttgaaa cacgcaggca ttcacaccga aaccgacgta
    10861 caaattacct ttgttgacag cgaaagcatc gagaaaaaca acggcgacgt ttctatgctt
    10921 aaagatatgg acgccatcct cgttcccggc ggttttggtt cgcgcggcgt ggaaggcaaa
    10981 atcgccgctg tgcgctatgc ccgcgaaaac aacgtgccat acttgggcat ctgcctcggt
    11041 atgcagattg cgctgatcga atacgcccgc gacgtggcag gtttgaaagg tgcgaattcc
    11101 accgagttcg acctcaaatg cgccgccccc gtcgttgccc tgattgacga atggcaaact
    11161 gccgacggca gcgtcgaaac ccgcgacgaa tccgccgatt tgggcggcac catgcgtttg
    11221 ggcgcgcaag aggttgagtt gaaagcaggc agcctcgccg ccaaaatcta cggcagcgaa
    11281 cacatccgcg aacgccaccg ccaccgctac gaagtcaaca acaactacgt tcctacgctg
    11341 gaacaagcag gtttggtcgt cggcggcgta tccgcaggac gcgaacgctt ggtcgaaacc
    11401 atcgaactgc cgaaccatcc ttggttcttc gcctgccagt ttcatcccga attcacttcc
    11461 aacccgcgca aagggcatcc cttgttcacc gcgtttgtca aagctgcgtt gaacaataaa
    11521 aaagcctgat aaagcgttac ttgatgatca aaatgccgtc tgaaagcctg acaaagactt
    11581 tcagacggca ttttcgcaag tcggggatta taaaagccat caatttaatt tttatggttt
    11641 tatctaatat ttaccaactg aatattgtta agcggggcgt gaaactcaag ttgagacctt
    11701 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggggat ttctcatgag
    11761 caccttcttt ca
//
LOCUS       DAWGJV010000056        11729 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWGJV010000056 DAWGJV010000000
VERSION     DAWGJV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.056"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(339..761)
                     /locus_tag="WHQ94_001080"
     CDS             complement(339..761)
                     /locus_tag="WHQ94_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ2128373.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WHQ94_001081"
     CDS             complement(879..1550)
                     /locus_tag="WHQ94_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ2128374.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..2192)
                     /locus_tag="WHQ94_001082"
     CDS             complement(1566..2192)
                     /locus_tag="WHQ94_001082"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ2128375.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="WHQ94_001083"
     CDS             complement(2299..3117)
                     /locus_tag="WHQ94_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2128376.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="WHQ94_001084"
     CDS             complement(3376..3816)
                     /locus_tag="WHQ94_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ2128377.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="WHQ94_001085"
     CDS             complement(3879..5285)
                     /locus_tag="WHQ94_001085"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ2128378.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5455..6570
                     /gene="lptF"
                     /locus_tag="WHQ94_001086"
     CDS             5455..6570
                     /gene="lptF"
                     /locus_tag="WHQ94_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ2128379.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6567..7637
                     /gene="lptG"
                     /locus_tag="WHQ94_001087"
     CDS             6567..7637
                     /gene="lptG"
                     /locus_tag="WHQ94_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ2128380.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7937..10522
                     /gene="acnB"
                     /locus_tag="WHQ94_001088"
     CDS             7937..10522
                     /gene="acnB"
                     /locus_tag="WHQ94_001088"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ2128381.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPHTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVHEGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10664..11659
                     /locus_tag="WHQ94_001089"
     CDS             10664..11659
                     /locus_tag="WHQ94_001089"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ2128382.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2692 a         3257 c         3044 g         2736 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttcg
      301 gacggcatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgcttg
      661 gccgcacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgttgttccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacgcctga aaatcgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtagg ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcaacagcca aaacccgggc ggcaacatcg tctgccgtat cgccgtcgag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggcaggacgg acgggtgaat gttcatcagc ctgccttcgt aatgggcgca aaactcaggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg cggtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctgtcg
     2041 gtcggaatac cctgaccggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tacggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccat
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cggaccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 accctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa aagattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgagc tcttccaggc tgttgccgac
     3481 gatttctaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cggacaggtt
     3541 caaaacggcc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaataa aactctcggc
     3661 ctcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgagcatt tggcaggacg
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccggtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgtgc ccatggatag tcttcggtaa agtaggatag gaatgttgcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atgtttggaa tgtcggcaaa gttggatttg
     4081 agttggtctt tgtaggtctc aaagagcggc agttgccacg ctttgtcgtc cacgttgtag
     4141 gaagcggcaa gcaggctgtc gaccaaatct tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg caacaataca cgcaccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgtt cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc agcatcggtg
     4321 ttcaacactt cgatggtcag acctttcata cttttcacga catcgcccgg tttgtttgcc
     4381 gcgccggaag gcatattttc acaagtggcg acgacggcaa tcaggttaat cggcagttgc
     4441 agtttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat atcaaacttc
     4501 atttcgtcca tgttcaggcc gggtttgagg gaaatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca ataccacagg cgcggcttct ttgtccgccg caccgaaata gctcagttcg
     4621 accaaatagg gatcttcgac actgcctttg gcgaccgacc aaaacgaacc catgttttct
     4681 ttgatgtagt ctttttcgat gattttggcg tgcgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttgcc caagtcgcgg
     4801 cagaggcttt gtccgtaaac ttgcgcttcg gcgacgcgca aggcttcttt aacgacggct
     4861 tcgtgtgcgc tgtggaacac ggcggtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc gttgccgaac gcaatcgcaa acgcttcggc tacggcggcg
     4981 gcttgcgctt catcgaaggc atggatgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa attttgacct
     5161 tcttcaagcg aagacaaaag ggcaagggcg gtcggatggt ttaattgcgc ctcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc ctgaccgcga ttctatgatt
     5461 tatcaaagaa acctcatcaa agaactctct tttaccgccg tcggcatttt cgtcgtcctc
     5521 ttggcggtat tggtctccac gcaggcaatc aacctgctcg gccgtgccgc cgacgggcgt
     5581 gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg tcatcggtat gacgccgctt
     5641 ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt tgacccgcta ctggcgcgac
     5701 agcgaaatgt cggtctggct atcctgcgga ttggcattga aacaatggat acgcccggtg
     5761 atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca tgcagctttg ggtgataccg
     5821 tgggcagagc tacgcagccg cgaatacgct gaaatcctga agcagaagca ggaattgtct
     5881 ttggtggagg caggcgagtt caacagtttg ggcaagcgca acggcagggt ttattttgtc
     5941 gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt tcctgcgcga acaggacaaa
     6001 aacggcggcg acaacatcat cttcgccaaa gaaggtaact tctcgctgaa cgacaacaaa
     6061 cgcacgctcg aattgcgcca cggctaccgt tacagcggca cgcccggacg cgccgactac
     6121 aatcaggttt ccttccaaaa actcaacctg attatcagca ccacgcccaa actcatcgac
     6181 cccgtttccc accgccgtac cattccgacc gcccaactga ttggcagcag caacccgcaa
     6241 catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca gcgtcctcct actctgcctg
     6301 cttgccgtgc cgctttccta tttcaacccg cgcagcggac atacctacaa tatcttgatt
     6361 gccatcggtt tgtttttaat ttaccaaaac gggctgaccc tgctttttga agccgtggaa
     6421 gacggcaaaa tccatttttg gctcggactg ctgcctatgc acatcatcat gttcgtcatc
     6481 gcaatcgtac ttctgcgcgt ccgcagcatg cccagccagc ccttctggca ggcggttggc
     6541 aaaagtctga cattgaaagg cggaaaatga acctgatttc acgttacatc atccgtcaaa
     6601 tggcggttat ggcggtttac gcgctccttg ccttcctcgc tttgtacagc ttttttgaaa
     6661 tcctgtacga aaccggcaac ctcggcaaag gcagttacgg catatgggaa atgctgggct
     6721 acaccgccct caaaatgccc gcccgcgcct acgaactgat tcccctcgcc gtccttatcg
     6781 gcggactggt ctccctcagc cagcttgccg ccggcagcga actgaccgtc atcaaagcca
     6841 gcggcatgag caccaaaaag ctgctgttga ttctgtcgca gttcggtttt atttttgcta
     6901 ttgccaccgt cgcgctcggc gaatgggttg cgcccacact gagccaaaaa gccgaaaaca
     6961 tcaaagccgc cgccatcaac ggcaaaatca gcaccggcaa taccggcctt tggctgaaag
     7021 aaaaaaacag cattatcaat gtgcgcgaaa tgttgcccga ccatacgctt ttgggcatca
     7081 aaatttgggc gcgcaacgat aaaaacgaat tggcagaggc agtggaagcc gattccgccg
     7141 ttttgaacag cgacggcagt tggcagttga aaaacatccg ccgcagcacg cttggcgaag
     7201 acaaagtcga ggtctctatt gcggctgaag aaaactggcc gatttccgtc aaacgcaacc
     7261 tgatggacgt attgctcgtc aaacccgacc aaatgtccgt cggcgaactg accacctaca
     7321 tccgccacct ccaaaacaac agccaaaaca cccgaatcta cgccatcgca tggtggcgca
     7381 aattggttta ccccgccgca gcctgggtga tggcgctcgt cgcctttgcc tttaccccgc
     7441 aaaccacccg ccacggcaat atgggcttaa aactcttcgg cggcatctgt ctcggattgc
     7501 tgttccacct tgccggacgg ctcttcgggt ttaccagcca actctacggc atcccgccct
     7561 tcctcgccgg cgcactacct accatagcct tcgccttgct cgccgtttgg ctgatacgca
     7621 aacaggaaaa acgttgaacc aatgccgtct gccccccttc ggacggcatt ggtttgtatt
     7681 atcatattcc cgtagacaga tagccgttcc ctattatagt ggattaacaa aaaccagtac
     7741 ggcgttgcct cgccttgccg tactattgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     7801 ttaaaataca ttacctgtca taacagttcc atttttgtta aaactagtct atgatagcgg
     7861 tacaaatatt gtttacaata tttaacgcaa atcatttgca acccgacaaa agaaaaacag
     7921 aaaaaggaac aaagagatgt tagaagccta tcgtaaagcc gccgccgagc gcgccgccct
     7981 tggtattccc gcccttcctc tgactgctca gcaaaccgcc gatttggttg agctgctgaa
     8041 aagcccgccc gcaggcgaag gcgagttctt ggtcgaactg cttgcccacc gtgttccgcc
     8101 cggtgtggac gatgccgcca aagtcaaagc ctcattcctg gctgccgttg ccgaaggcag
     8161 cgcgtccagc ccgctgatct cccccgaata tgcgaccgaa ctcttaggta cgatgctcgg
     8221 cggttacaat atccacgcct taatcgaact cttggacgac gacaaactcg cgcccattgc
     8281 cgccaaaggc ttgaaacata cgcttctgat gttcgattcc ttccacgacg ttcaggaaaa
     8341 agccgaaaaa ggcaacaaat acgcgcaaga agttttgcaa tcttgggcag atgccgaatg
     8401 gttcgcctca cgcgccaaag ttcccgaaaa aatcaccgtt accgttttca aagttgacgg
     8461 cgaaaccaat acagacgacc tctcccccgc gcccgacgcg tggagtcgtc ccgatattcc
     8521 gctgcacgcg ctggccatgc tgaaaaaccc gcgcgacggc atcacgcccg acaaaccggg
     8581 cgaagtcggt ccgattaaat tgttggaaga actcaaagcc aaaggccatc cggttgctta
     8641 cgtcggcgac gtggtcggta ctggttcttc acgcaaatcc gcgaccaact ccgtcatttg
     8701 gcataccggc gaagacattc cgttcgtgcc gaacaaacgc ttcggcggcg tatgtttggg
     8761 cggcaaaatc gcgccgattt tcttcaatac ccaagaagat tccggcgcgc tgccgattga
     8821 agtcgatgta tctgctctaa aaatgggtga tgtcgtcgat atcctgcctt atgaaggcaa
     8881 aatcgtgaaa aacggcgaga ctgttgccga gtttgaattg aaatcacaag tattgctgga
     8941 cgaagtgcaa gccggcggcc gtatcaacct gattatcggc cgaggtctga ccgccaaagc
     9001 gcgcgaagcc ctgaaactgc ctgcctctac tgcattccgc ctgccgcaag cgcctgccga
     9061 aagcaaagcc ggtttcacct tggcgcaaaa aatggtcggc cgcgcctgcg gtctgcccga
     9121 aggacaaggc gtgcgtccgg gtacttactg cgaaccgcgt atgacgacgg tcggctcgca
     9181 agacacgacc ggcccgatga cccgcgacga gttgaaagac ttggcttgtt tgggcttctc
     9241 cgccgatatg gtgatgcagt ctttctgcca caccgccgcc tatccgaaac ctgtcgatgt
     9301 aaaaacccat aaagaactgc ccgcctttat ttccacccgt ggcggcgtgt cactgcgtcc
     9361 gggcgacggc gtcatccact cgtggctcaa ccgcctgctg ctgcccgata ccgtcggcac
     9421 tggcggcgac agccataccc gtttccccat cggtatttcc ttccccgccg gctccggctt
     9481 ggttgccttt gccgccgcaa cgggcgtaat gccgctcgat atgcctgagt ctgtattggt
     9541 acgcttcagc ggcaagctgc aaccgggcgt aaccctgcgc gatttggtga acgccatccc
     9601 gctgtacgca attaaacaag gtttgctgac cgttgccaaa gccggtaaga aaaacatctt
     9661 ctctggccgc atcctcgaaa tcgaaggcct gcccgatttg aaagtggaac aagcctttga
     9721 attgaccgac gcatccgccg aacgctccgc cgccggctgt accgtgaagc tcaataaaga
     9781 gccgattatc gagtacatga aatccaacgt cgtgttgatg aaaaacatga ttgccaacgg
     9841 ctatcaagat ccgcacactt tggaacgccg catcaaagct atggaaaaat ggctggcaaa
     9901 tcccgagttg ctcgaagcgg ataaagatgc cgaatacgcc gccgtgattg aaatcaacat
     9961 ggacgacatc aaagagccga ttatcgcctg cccgaacgat ccggacgacg tatgcttcat
    10021 gtccgaacgt tccggcacca aaatcgacga agtgttcatc ggttcgtgca tgaccaacat
    10081 cggccacttc cgcgccgctt ccaaactctt ggaaggcaag agcgacatcc ccgtccgctt
    10141 gtgggttgcg ccgccgacca aaatggacgc gaaagagttg tccgacgaag gccactacgg
    10201 tgtgctcggc cgtgcaggcg cgcgtatgga aatgccgggt tgctcattgt gtatgggtaa
    10261 ccaagcccaa gtacacgaag gcgcaaccgt catgtccact tcgacccgca acttcccgaa
    10321 ccgcttgggc aaaaacacct ttgtttacct cggttcggcg gaattggcag cgatttgctc
    10381 caaactgggt aaaatcccga ccgttgaaga atatcaagcc aatatcggca tcatcaacga
    10441 acagggcgat aaaatctacc gctatatgaa cttcaacgaa atcgacagct acaacgaagt
    10501 agccgagacc gtgaacgttt aatccccgtc atccgtatga agtaagggat tgaccgcaat
    10561 gccgtctgaa caaccttcag acggcattgc aacattccgc taacccttct ttccgcaaac
    10621 gctgcaaata cggcgttcac gcccccacat aaaggaaacg acagtgaacc tgaaaaaccg
    10681 ccattttctg aaacttttag acttcacgcc ggaagaaatc accgcctacc tcgaccttgc
    10741 cgccgaattg aaagccgcca aaaaagcagg gcgcgagatt cagcggatga aagggaaaaa
    10801 catcgccctg atttttgaaa aaacctctac tcggacgcgc tgcgcgtttg aagtcgccgc
    10861 gcgcgatcaa ggcgcgggag tgacttattt agagccgtcc gccagccaaa tcgggcataa
    10921 ggaaagcatc aaagacaccg cccgcgtgtt gggcaggatg tacgatgcca tcgaatatcg
    10981 cggtttcggt caggaagttg ttgaagaatt ggcgaaatac gcgggcgtac ccgtgttcaa
    11041 cgggctgacc aacgagttcc atcccacaca aatgcttgcc gacgcactga ctatgcgcga
    11101 acacagcggc aaacctttga accaaaccgc gtttgcctac gtcggcgacg cgcgttacaa
    11161 catgggcaat tccctgctga ttttaggggc aaaattgggg atggacgtgc gtatcggcgc
    11221 accgcaaagc ctgtggccgt ctgaaggcat tattgccgcc gcacacgccg ccgccaaaga
    11281 aaccggcgca aaaattaccc tgaccgaaaa cgcgcatgaa gccgtgaaga atgttgattt
    11341 tattcatacc gatgtgtggg tcagcatggg cgagccgaaa gaagtctggc aggaacgcat
    11401 cgatttgctg aaagattacc gcgttacgcc cgaactgatg gcggcatcgg gcaatccgca
    11461 agtcaaattc atgcactgcc tgcccgcctt ccacaaccgc gaaaccaaag tcggcgaatg
    11521 gatttacgaa accttcgggc tgaacggtgt ggaagttaca gaagaaatat tcgaaagccc
    11581 cgccagcatc gtgttcgatc aggcggaaaa ccgtatgcac acgattaaag cggtaatggt
    11641 cgcggctctg ggcgactgac agaactgtgc ctgtttaaat tcatccgcaa cacagatacc
    11701 gtctgaacac gatgttcaga cggtatcca
//
LOCUS       DAWGJV010000057        11326 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWGJV010000057 DAWGJV010000000
VERSION     DAWGJV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.057"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(221..1018)
                     /locus_tag="WHQ94_001090"
     CDS             complement(221..1018)
                     /locus_tag="WHQ94_001090"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ2128383.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVAYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFILFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAVAAKIQAAVQDVLKD"
     gene            1173..4019
                     /gene="uvrA"
                     /locus_tag="WHQ94_001091"
     CDS             1173..4019
                     /gene="uvrA"
                     /locus_tag="WHQ94_001091"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ2128384.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(4051..4221)
                     /locus_tag="WHQ94_001092"
     CDS             complement(4051..4221)
                     /locus_tag="WHQ94_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128385.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(4236..4433)
                     /locus_tag="WHQ94_001093"
     CDS             complement(4236..4433)
                     /locus_tag="WHQ94_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128386.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            complement(4437..4706)
                     /locus_tag="WHQ94_001094"
     CDS             complement(4437..4706)
                     /locus_tag="WHQ94_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128387.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            4844..5785
                     /locus_tag="WHQ94_001095"
     CDS             4844..5785
                     /locus_tag="WHQ94_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ2128388.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(6012..6278)
                     /locus_tag="WHQ94_001096"
     CDS             complement(6012..6278)
                     /locus_tag="WHQ94_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128389.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            6527..6856
                     /locus_tag="WHQ94_001097"
     CDS             6527..6856
                     /locus_tag="WHQ94_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2128390.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(6922..7062)
                     /locus_tag="WHQ94_001098"
     CDS             complement(6922..7062)
                     /locus_tag="WHQ94_001098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128391.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFEAGRIFIGQTGA
                     NP"
     gene            complement(7091..7981)
                     /gene="sucD"
                     /locus_tag="WHQ94_001099"
     CDS             complement(7091..7981)
                     /gene="sucD"
                     /locus_tag="WHQ94_001099"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ2128392.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(7992..9158)
                     /gene="sucC"
                     /locus_tag="WHQ94_001100"
     CDS             complement(7992..9158)
                     /gene="sucC"
                     /locus_tag="WHQ94_001100"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ2128393.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(9257..9544)
                     /locus_tag="WHQ94_001101"
     CDS             complement(9257..9544)
                     /locus_tag="WHQ94_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128394.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(9669..11102)
                     /gene="lpdA"
                     /locus_tag="WHQ94_001102"
     CDS             complement(9669..11102)
                     /gene="lpdA"
                     /locus_tag="WHQ94_001102"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2128395.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGGCRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2844 a         2970 c         2586 g         2926 t
ORIGIN      
        1 gttttttaag ttttaggtaa ctaatgagtt gtcattcccg cgcaggcggg aatccaattc
       61 gttcggtttc agccgtttcc gataaatcgc tgtaacgtta agttcccgga ttcccatttt
      121 cgtgaacata acggtgtgaa cattagcaaa aattcaaaaa accaaacttc ctcattcgtg
      181 tgaatgatga tactgatatt ttctatttca gttcgcaaaa ttaatctttc aacacatctt
      241 gcacagccgc ctgaatctta gccgccactg cctcgatttg ccgttgttcg gcagatgggt
      301 ttgccgctgt ttcaaccgga gcagtttgta aagcaggctc agattcaggc tcggattcga
      361 tttgcggcgc agtcagcggc aaacgcgcca atacagtgct gacaccgttt actttatcgc
      421 caatcgccac ttgcgcctgc gcatcgacag gcagatacat atccacgcgc gaaccgaaga
      481 ggataaagcc ataacgttcg ccgcgggaca gtttcgcacc tgcttgggtg tagcacaaaa
      541 tacggcgcgc caccaaaccg gccacttgaa caaaagtaat ttcacgaccg gaagccgtag
      601 tcgccaacac cgcattacgt tcgttttccg tgctggcttt gtccaaatcc gcattcacga
      661 atttgccttt gttataggcc actttcgtta ccgtacaatc ggcaggcgat ttttgcgaat
      721 gcacgttgaa cacgttcata aaaatactga ttttcaaagc atcgacatca cgatacggat
      781 cgcgtgcgcg ctcgaccacc acgatacggc cgtcaacagg actcaacacc gcttcaggat
      841 tttgcggaat ctcacgcgca gggtcgcgga aaaactgcaa agcaaatacg gtaaacaccc
      901 aaaacggcaa agaccaccag ccgcaacaca tcgacaccag caggctcaaa gccaaaccgc
      961 cgccaataat cggccagccc tcacgggcga taatcgggtg ggggtaaaga cggttcattt
     1021 gtcatccttt tgttcaagcg ggttaaaatt gccgccatta taacggaatc tgccgcgttc
     1081 cgccgacaaa tgccgtctga acgcggcttc agacggcatc cgagaaagtg ctacaatatc
     1141 ggttttcctt tatttttcag gcggcatcca ttatgtgcaa ccatcatccc caacattcgc
     1201 acgacaacga caccatccgc atccgcggcg cgcgcacgca taatttgaaa aacatcgatt
     1261 tggacattcc gcgccacaag ctcgtggtgg taacaggatt gtcgggcagc ggcaagtcgt
     1321 cgctggcatt tgatacttta tatgccgaag ggcagcggcg ttatgtcgag agcctttctg
     1381 cctatgcgcg gcagtttttg cagatgatgg acaagcccga tgtcgatttg atcgaaggct
     1441 tgtcgcccgc gatctccatc gagcagaaat ccaccagcca caatccgcgt tccaccgtcg
     1501 gcacggttac ggaaatccac gactacctgc gccttttgta cgcccgtgtc ggtacgccgt
     1561 attgtcccga acacaatctg ccgctatcca gccaaacagt atctcagatg gttgatgccg
     1621 tgttgaagct gccggaagac acgcgcgtga tgattctggc gccggcggtg cgcgagcgta
     1681 agggcgagtt tgtcgacttc tttgccgact tgcaggcgca gggttttgcg cgcgtgcgcg
     1741 tggacggcga agtttatcag ttggacgaag taccgaagct ggaaaaaaac atcaagcaca
     1801 atatcgacgt ggtcatcgac cgcgtgaaag tgaaggcgaa catcaagcag cggctggcgg
     1861 aaagttttga aaccgcgctg cgccacggca acgagcgcgc gctggcgatg gaaatggaca
     1921 gcggcgaaga gcattggttt tccgcgcgtt tcgcctgccc cgtatgttcg tacagcctgc
     1981 ccgaattgga gccgcgcctc ttttcgttca acaacccgat gggctcctgc ccgacttgcg
     2041 acggcttggg taacaccaat ttcttcgact ccgaaaaagt ggtcgcgcat cccgaattgt
     2101 cgctggcttc gggcgcgatt gacggctggg acaagcgcaa ccagttctat ttccaaatga
     2161 ttcagtcgct ggcgcggcat tacggtttcg acgtgcaggc tgcttgggaa accctacctg
     2221 caaaaatcaa aaaagtcgtg ttgcacggct cgggcaaaga agtcattgat ttcacttacc
     2281 tgtccgaacg cggcaccacc ttcaaccgca gccacgcctt cgaaggcatc atccccaatc
     2341 tcgaacgccg ctaccgcgaa accgacagcg aaaccgtgcg cgaaaaactg cgcgaatacc
     2401 aaaaccaccg cgcttgcccg agctgcggcg gcgcacgttt gcgcaaagaa gcccgctacg
     2461 tttacgtcgg cggcgagccg ttgcacgaag tttccgcctg gccgctgacc aaaacccacc
     2521 gattctttga aacgctggat ttggacggca acaaaaaaca aatcgccgaa aaaatcctca
     2581 aagaaatcac cgaacgtctc ggctttttga ttaatgtcgg gctggattac ctgaatctct
     2641 cccgttccgc cgaaaccctt tccggcggcg aagcccagcg catccgcctc gccagccaaa
     2701 tcggcagcgg cctgaccggc gtgatgtacg ttttggacga accctccatc ggcctgcacc
     2761 agcgcgacaa cgaccgcctg cttgccaccc tcaaacgcct gcgcgatttg ggcaacagcg
     2821 tcatcgtggt cgaacacgac gaagacgcca tccgcgaagc cgatttcgtc gtcgatatgg
     2881 gcccgggcgc aggcgaacac ggcggcaacg tattgattgc cgacacgccc gaaaacgtcg
     2941 ccaaatgcga aaactccgtt accggacaat acctcagcgg caaaaaatcc attgccgtgc
     3001 cgtctgaacg cacgccagtc aatcccgacc gaatgctcgt cctcaaaggc gcgcgcggca
     3061 acaacctcaa aaacgttacc cttgagctgc cgctcggttt gattacctgc atcaccggcg
     3121 tatccggcag cggcaaatcc accctgatta acgacaccct cgccaaaatc accgcccgcg
     3181 aactcaaccg cgcccaagaa gaacccgccc catacgacga catccgcggc ctcgaacacc
     3241 tcgacaaagt catcaacgtc gaccaatccc ccatcggccg caccccgcgc tccaaccccg
     3301 ccacctacac cggcctgttc acccccatcc gcgaactctt cgccggtgta cccctctcgc
     3361 gcgaacgcgg ctacaacgtc ggcagattct ccttcaacgt caaaggcggc cgctgcgaag
     3421 cctgccaagg cgacggcgtc atcaaagtcg aaatgcactt cctgcccgac gtgtacgtcc
     3481 cctgcgaagt ctgccacggc aaacgctaca accgcgaaac gctcgaaatc caatacaaag
     3541 gcaaaaacat cagccaagtc ctcgacatga ccgtcgaaga agcccgtgaa tttttcgacg
     3601 ccgtccccac cgtatcgcgc aaactgcaaa ccttgatgga cgtaggctta ggctacatcc
     3661 gtctcggcca atccgccacc accctctccg gcggcgaagc ccagcgcgtc aaactcgcct
     3721 tggaactctc caaacgcgac accggcagaa cgctctacat cctcgacgaa cccaccaccg
     3781 gcctacactt cgccgacatc gccctgctgc tggaagtcat agaccgtctg aaaggcaaag
     3841 gcaactcgat agtgattatc gagcataatc tggatgtgat taaaaccgcc gattggattg
     3901 tggacttagg gccagaaggc ggcgacggcg gagggaggat tattgcttca ggtagcccgg
     3961 aggaagtggc gaaggttaag gggagttata ctgggaagta tttgaagaat atactttaaa
     4021 aaacgggatg gtttaaacca tcccgttttt ttattccttc tgttttcgat tttgtttggc
     4081 tttgaagcta tcaaaaataa tgctaaatcc caaacaggtg aacattaaag gcatccccca
     4141 tttcatatcc gaagaaataa cattaaacat ttctaataat gtaagaatgc ctgataaagc
     4201 tataaaaata cctaaaaaca ttagaactat ccttattatc tggagctatc tggagttctt
     4261 ttaatttgaa tccatgagct tcctggattt tgagtgggtg gtgctttttg attatcttta
     4321 atggttgcgt agttatcttt tcgaccacct cgtgaaccaa tttcttgata aattccaccg
     4381 ttatttcctg tattttgacc aggtttgatt ttgttcgtca ttatcatctc cataatttaa
     4441 atagttaaag tatgagtcca ttgcggatac caatttgcag ataaaaaatc tactagattt
     4501 ttgtctccat agaattgtgt acgcccatgt tgttcaaata ttaacgcaga ttggtcaatt
     4561 tttaatccat ttctacgagc ttcagtggtc aattccttca gtacgacact atggttacca
     4621 ttagttgatt tagcatcaaa aacagcaaag ttaatatatc ctccacctac ggctggatgt
     4681 cttaagtgag caaattttat tttcataaat aaatcctata aaaaattaca taacagaaat
     4741 tttaagtcag agagctacta tgaagtagct ttttgcatta tatgcactct ttttgattac
     4801 gtcaagcaat ttttttgaat tcatattgcc atgaaaaaga ttgatggaat ataatgcaat
     4861 tatgaaaaca atcgctcctt cccatataaa gccccttatg ccagagttga accaatctca
     4921 acgcaatagg attatattta ttgatttcta tctatgcttt ctaggaaaga taaatagaaa
     4981 agatttgatg caggcatttc aaatttcccc agctgttgct actagggatt ttgcgctata
     5041 ttgctctagt ttttcgaaaa atctccgttt tgataccaaa agtaaatttt acctctctac
     5101 tgaagacttt gaacctcaat tcccccattc atttgaaaat gcaattaaaa ttcttaatgg
     5161 cagtttaata tttgatattg attcccaaca tcaacctctt cttccgtttg atttacctcc
     5221 tgttttaata aatcctgatg tcttaatctt ttctaaaata tcgaaagcat tatccttaaa
     5281 aaaacctcta aaaatctcat atcattcaat ttcatctgga ttatctgaga aagttattgt
     5341 gcctcttgct ctagttaatg acggatttcg atggcattta cgggcattcg atagaacaaa
     5401 aaaccgattt gcagattttg tcctaacacg aataattcaa gcaaatattt tattagctga
     5461 aaatattgaa gaaaatgaaa cgattaacaa tgatattgat tggaatagaa ttgttaaaat
     5521 cgagttggtt ccccatccta atcatgatga agctaaaaaa gtgattgaaa tggactttgg
     5581 aatgacaaat ggaaaattag aattggcgct tagagcttca aatatcggat atgttttaca
     5641 gcaatggcga gtcgattgtt cgtcagatca tcatatcgat gatcctgctt ttagattatg
     5701 gctgttagat aatttagttt tatacggcat caacaatgct gaacacttag cgcctgcctt
     5761 tgataaaaaa cgtagcaagc cgtagcctac atatccaacc gaccgcgtgc gtggctgcgc
     5821 cacacacgcg gttgtttgat tgatggcttt ctgttgtaaa ttgaacttag ttaaaactaa
     5881 cactttcaga cggcatgaaa aatggccgtc tgaaaactga aaaagcttta tctaaaacaa
     5941 ctaccctaac tcacaaaaca aaaaacggat tcccaatcat tggaatccgt ttttctgtaa
     6001 agctatccga attatttagc ttcttcttta gcgtcttcag cagcttcttt ggcatcttcc
     6061 gctgcgtctt ttgcttcttc tttagcctct tcagcggcgt ctttggcttt ttcttcagga
     6121 gtttctgcag cagcaagccg tagcctgcat acccaacacc gcgtgcgtgg ctgcgccaca
     6181 caccctacct gtgaattaca cggaacttca aaatcaacgt gtagggtgtg tgcggtacgc
     6241 acgcacgcgg ttgcttgatt gacggtttgc cgtttcaact tttaacttcg ttgaagctag
     6301 cgctttcaga cggcctccaa tgtcgtctga aagcgctagc ttcaacgaag ttaaaaacct
     6361 taaacaacaa gccattcctt ctccaacgct caaaccaaaa acaaaaatcc tatggcgcgt
     6421 tatcgcagaa acttcattgc cggcggcaca ttctttttca ccgtcaaact cgccgacccg
     6481 aaatcgcgcc tgcttgtcga acatatcgac ttcgcgggcg gcttatatgg atgtgcaaaa
     6541 acaatattcc tttgaaaccg tcgccgtatg cgtgctgccg aaccatattc acgccatttg
     6601 gacgctgccg cccgacgatg cggattattc cctactccgg cggctgatta aaaccaaatt
     6661 ctccgcatat tccccttata ctaaaaactt atccgccggc aaacagcggc agcacgaacg
     6721 cggtatttgg caacggcgtt tttacggaca caccgtgcgc gacgaaacgg atttgcagcg
     6781 ttgcgcggac tacatccatt ccaatcctat caaacaaaaa atgtcgcgtg ctaaaacaga
     6841 atcctgcaaa aaataatatc agtcttttca aatgcgatag ttatcattcc ccaacttcaa
     6901 acaaggcgtg tgcaacctga cttacgggtt tgctcctgtt tgaccgataa atattctgcc
     6961 tgcctcaaag ggcaaaaata atgtgggtat gttgaggctg cctctttttg aaatgacaaa
     7021 caaaatgccg tctgaaaccc gatttccggt ttcagacggc atttgatgtt caatcagttg
     7081 tcaaacctga ttatgccaaa ccttttgttt tcaacacttc cagcatggta gtgcccaact
     7141 cggcagggct gcgggtgtaa gcgataccgg ctttttcaaa cgcggcgaat ttttcctcgg
     7201 cagtaccttt gccgccggaa ataatcgcac cggcgtggcc catgcgtttg cctttaggag
     7261 cggtaacacc ggcgatgtag ccgacaacag gtttgcttac gttggattgg atgtattcgg
     7321 ctgcttcttc ttccgcagta ccgccgattt caccgatcat gatgatggcg tcggtatctg
     7381 ggtcttcttg gaaaagtttc agtgcgtcga tttggttcat accgggaatc gggtcgccgc
     7441 cgataccgat acaggttgat tgacccaagc ccagtttggt ggtttgtgcc acggcttcgt
     7501 aagtcaatgt accggaacgg gaaataatac cgatgcggcc cggagtgtgg atgtggcccg
     7561 gcataatacc gattttgcac tcgcctgggg taatcacgcc cgggcagtta gggccgacca
     7621 agcgggtacc gttgccgttg gtttccaagt aacgtttggc tttgagcatg tccagagtcg
     7681 gcacgccttc ggtaatcaca accaccaagc ctacgccgga atcaacggct tcaacgatag
     7741 aatccaacac aaacggagcg ggaacgtaaa ttacagatgc atccgcgccg gtttctttaa
     7801 cggcttcttt catggtgttg aacacgggca ggttcaggtg ggtttgaccg cctttgcccg
     7861 gggtcacgcc gccgacaact ttagtgccgt aagccagagc ttgttcggag tggaaagtac
     7921 cgtttttacc ggtgaaacct tgaaccaata ctttggtgtc tttattaatc aatacgctca
     7981 ttcttttctc cttaggcgtt tacggctgcg acaatttttt cggctgcgtc attcaggccg
     8041 tctgcagaag tcagtttcag acctgattcg ttcaggattt tcgcgccgag ttcggcgttg
     8101 ttgccttcca aacgaacaac gacaggaacg tcgacgttga tttctttaac ggctgccacg
     8161 atggcttccg caatcatgtc gcaacgtacg ataccgccga agatgttgat caatacgcct
     8221 tgaacggatt tgtcttccag aatcagtttg aacgcttcaa ccacgcggtc tttggttgcg
     8281 ccgccgccaa cgtccaagaa gttggcaggt tggccgcctt tcagtttgat gatgtccata
     8341 gtggccatcg ccaaaccggc accgttcacc atacagccga tgttgccttc cagggcaaca
     8401 tagttcaggt caaattcaga agctttcaac tcgcgttcgt tttcttgaga tttgtcgcgc
     8461 aattcggcga ttttcggcag gcggtagagc gcgttgctgt cgatgccgat tttgccgtcc
     8521 acgcaggcga gcgcgccgtt ttcgcgaact gccagcgggt ttacttcaaa cagggcgaag
     8581 tcattttcga caaacgcttt gtacgcaccg gtcatcagtt tgacgaactc gttgatttgt
     8641 ttgtctttca agcccagttg gaacgcaact tcgcgagctt ggcaaggttg caggccgacc
     8701 agcggatcaa cggttacttt gaagattttt tcaggagttt cggcagcaac tttttcgatt
     8761 tccacgccgc cttcggtaga ggccatgaat gtaatgcggc gggtagaacg gtcaaccact
     8821 gcgcccaagt acagctcggt ttgaaccgga tacatgtctt cgcaaaccaa aacactgttg
     8881 acaggttggc cgttggcatc ggtttggtaa gttaccaagt tggtgccaat caggctttca
     8941 gccacttctt tagcttcttc gcggctttta acgactttta cgccgcccgc tttaccgcgg
     9001 ccgccggcgt gtacttgtgc tttgacaaca gcgaatttgc cgcccaattt gtcgtaagct
     9061 gcagcggctt cttcgccgtt gtgtgccaaa ataccgcctt gtacgggcaa accgtagcta
     9121 gccagcagtt ctttagcctg atactcgtgt aaattcatgg atttctcctt tagattagtg
     9181 gaatgccccg agtctgcctc caaatggcaa gcccgtgcgt ttcagactac ctttcggcgg
     9241 tcggaaaatc gtcgtttcac ttgctgaaag gcaaccacat ccgcgccagc agatacatgg
     9301 ctgcaccgcc tcccccgaga ataaaaaaga cgatgccttt tttacgcgaa ttcggacaga
     9361 gcagataaac ggcaaggctg aaactgataa acaacaatgc cacgcgccaa accaattctt
     9421 tatctttgaa acggttgagc gtataagttt cggtcatcat cacatacatc tgccacaatg
     9481 cggccataca catgctgatg atgcccatcg gtataaagaa aataccgact atttctttgt
     9541 tcataacgaa tgtaaatgct gtttgtttgt ctgacggaat tataaatcag aaaacggagc
     9601 tctcggaacg ttcaatttgt cgaaggcatt aaggtcgtct gaaaaatttc agacgacctt
     9661 aatgtctatc aaccgtgcaa agcgcgtttg tcggccgcca atgcagcttc gtgaaccact
     9721 tcggacaagg ttgggtgggc atggataatg cgggcgatgt cttcgctgct ggcgaagaat
     9781 tcgagcgcag tcacgccttc ggtaaccaat tcgctgacaa ccgggccaat catgtgtacg
     9841 cccaagatgc ggtcggtttt ggcatctgcc aatactttaa ccgtaccttt ggctttgccc
     9901 attgccaatg cgcgaccatt cgcaccaaaa cctgaagtac cttttttgta ctccacgcct
     9961 tcggctttga gctgctcttc ggttttaccc acccaagcga tttcaggatc ggtgtaaatc
    10021 acgaacggta cgttgttgaa gtcgatatgc ggtttttgac cggcaatgcg ttcggcaacg
    10081 gcaacgcctt cgtcgctggc tttgtgtgcc aacatcgggc cgcgaaccac gtcgccgatt
    10141 gcccatacgt taggcaggtt ggtacggcat ccgccatcta ctttgataaa gccgcgctcg
    10201 tctttttcca agcctacggc ttccgcgttc aggcctttgg tgtttggaat acggccgatg
    10261 gcaacgatca gtttgtcgaa tacttcggtt ttggcttcgc cggcagcagt ttcgtaagca
    10321 acggaaacac ctttgccttc agacttgatg tcgccgattt tcacgcccaa ttcgatgctc
    10381 agaccttgct ctttggtgaa gtatttgaag gcttctttgg cgatttgttg gtcggcggca
    10441 gccaggaagg tcggcgcggc ttcaagaatg gtaacttccg cacccacgcg gttccatacg
    10501 gaacccattt ccaaaccaat cacaccggaa ccgatcacgc cgagtttggc aggtacttcg
    10561 gtcaggttca atgcgccttc gttgtccaat acgttcacat tgtcgatagc gacttgtggc
    10621 agcggacgcg gtacggaacc ggtcgctacg atgacgtgtt tggcttcgat aacggttttc
    10681 tctcctttgt tatcgacttc gatttggtaa gcatcgccat ttttaccggc aaaggaagcc
    10741 gtaccgaaca gactggttac tttatttttt tggaacagga atttcacgcc gccggtcagt
    10801 ttggtcacga tggcatcttt gcgctcaatc attttggccg cgtcgaattt tacgtcaccg
    10861 acagtgatac cgtgttcggc aaaatcgtgt tgcgcagcgt ggaaatgttc gctggattgc
    10921 aacagggctt tagaagggat acaacctacg ttcaagcaag taccgcccaa tgcaggggca
    10981 ttgcctgctt tgttaacgcc tgcatcgaca caggcagttt tgaaacccag ttgtgcggca
    11041 cggatggcgg caacgtatcc gcccgggcct gcaccaatca ctactacatc atattgagac
    11101 atcgtgtcat cctttgcttt tagcagtatc tcaaaataaa aacgatactt ttcgtttctt
    11161 ttttattttg aatgactgcg ttttgatggt tttaagaaaa agccgtctga aagtccatat
    11221 ctgccgattc attcgaatca aatgtttcag acgacctcaa tcgttcgttg agcctattgt
    11281 agcacgatac tacattacgc tacatctaaa catagtggat taaatt
//
LOCUS       DAWGJV010000058        11312 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWGJV010000058 DAWGJV010000000
VERSION     DAWGJV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.058"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            56..338
                     /locus_tag="WHQ94_001103"
                     /pseudo
     CDS             56..338
                     /locus_tag="WHQ94_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(359..1585)
                     /locus_tag="WHQ94_001104"
     CDS             complement(359..1585)
                     /locus_tag="WHQ94_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ2128396.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1600..2025)
                     /locus_tag="WHQ94_001105"
     CDS             complement(1600..2025)
                     /locus_tag="WHQ94_001105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128397.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2792
                     /gene="queC"
                     /locus_tag="WHQ94_001106"
     CDS             2130..2792
                     /gene="queC"
                     /locus_tag="WHQ94_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ2128398.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYRRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDEAAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            2820..3338
                     /locus_tag="WHQ94_001107"
     CDS             2820..3338
                     /locus_tag="WHQ94_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ2128399.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3345..3767
                     /gene="queD"
                     /locus_tag="WHQ94_001108"
     CDS             3345..3767
                     /gene="queD"
                     /locus_tag="WHQ94_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ2128400.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4036..4407
                     /locus_tag="WHQ94_001109"
     CDS             4036..4407
                     /locus_tag="WHQ94_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ2128401.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4404..5042
                     /locus_tag="WHQ94_001110"
     CDS             4404..5042
                     /locus_tag="WHQ94_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ2128402.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(5378..5454)
                     /locus_tag="WHQ94_001111"
     tRNA            complement(5378..5454)
                     /locus_tag="WHQ94_001111"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5418..5420),aa:Pro,seq:cgg)
     gene            complement(5682..6767)
                     /gene="nagZ"
                     /locus_tag="WHQ94_001112"
     CDS             complement(5682..6767)
                     /gene="nagZ"
                     /locus_tag="WHQ94_001112"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ2128403.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6824..8344)
                     /locus_tag="WHQ94_001113"
     CDS             complement(6824..8344)
                     /locus_tag="WHQ94_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ2128404.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8547..10046)
                     /locus_tag="WHQ94_001114"
     CDS             complement(8547..10046)
                     /locus_tag="WHQ94_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ2128405.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGKEITVAVKLGN
                     ASEQTGSSSEPDKAPYAEHQSGTFSVESAGITLQTHTDSSGGRLVVVRVSGAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10184..10813)
                     /gene="nth"
                     /locus_tag="WHQ94_001115"
     CDS             complement(10184..10813)
                     /gene="nth"
                     /locus_tag="WHQ94_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ2128406.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10859..11272)
                     /locus_tag="WHQ94_001116"
     CDS             complement(10859..11272)
                     /locus_tag="WHQ94_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2128407.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2661 a         3040 c         3145 g         2466 t
ORIGIN      
        1 ttttttcgat acggaaccgg atagaggaaa aatcgaacat tgcgcctgcc ttgctatgat
       61 tcacgaagaa atctccgcca tgcctatggg ctatgaaacc ttgatcggcg atatgggcag
      121 cgcactgtca ggcggacaaa aacaacgcat cgtattggcg cgggccttaa tattgcgaac
      181 cgaaaatcct atttttagat gcagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattact gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tatagccgta ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 tgaagaggga gcgcgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacacg tttgcctgta taatccgcat ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggcca agattccact acctgcctga ttcaggcgat tcaaacttac agacgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgatttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaag ccgccgccat cgaaaccgcc ggcaacggtg
     2401 ttcccaatac ctttgtggac gggcgcaacg cgcttttcct gctttacgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acttgggcgt
     2641 tggcggacga aatgggcgca ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggcggctgc cgcgaatgcc cgagctgtat cttgcgcgaa cgcgggctgg
     2761 cggaatatct ggaaagtaaa aaggccgtct gaacacgcgc aaaccataag gaatacgata
     2821 tgcccaagct ccatatgttt tacctcggcg gcaatgccgg caggtcgaat atcgaagtgc
     2881 acgacatcca atttgccgtg tgcgacgact accgtgaagc cgtccccgca ctcaaagccg
     2941 cgtggttcgg cgatgcggac aaaatccaca tcgacggctg gcagattgtc gaatgggcgg
     3001 acggttacga catcgccgta tccgaaacgc ccaaaacgaa aatgccgtct gaacacgccc
     3061 cgcgcctgta ttttgccaat gtcggcggtt atcgcgcagg gcagctcgcc gaagcgcacg
     3121 cttttgggct gttcgccgcc gccacgcctg ccgaagccaa acaaaaagcc ctgcaaaccc
     3181 tgttgaccga cagctatgtt cagcaacata aagacaactt aaaagacgtg gacaacctgc
     3241 ttgcgctcga ccgcatcggc aatttccata tccgcctgac cccgaatccg cacggcaaac
     3301 ccgccgaaat cggctttcaa ggctatttgc ccatttgaga acccatgaaa atcaccaaaa
     3361 tcttcacctt cgactcctcg catatgctcg acgggcatga cggcaaatgc caaaacctgc
     3421 acgggcatac ctacaaactc gaaatcaccg tttcagacga ccccgtcaga ggcgggccga
     3481 aagacggtat ggtgatggac tttaccgact tgaaagccat tgtcaaacaa cacattaccg
     3541 accccttcga ccacgccttc atctaccacg gcggcaacgg gcgcgaatct caaatcgccg
     3601 cactcttgga aggctggaac atgaaaaccc tgcgcctgcc ctgccgcacc actgccgaaa
     3661 atatggcggt cgaaatgtac gaccgtctga aaaacgcggg gctgaacgtg tgccgcgtca
     3721 aattgtggga aacgccgaca tcgtgtgcgg agtatgaagg ggagtaggga atatcttgaa
     3781 cgtatcgata tagtaaattc caataagaca tgcccaaccg cgtcattccc gcgcaggcgg
     3841 gaatccagac cttgatttat caggaatatt taaaaattgc agcaattcca actctctgga
     3901 ttcccgcctg cgcggaaagg acggtttaga gcgtccttat ttgaatttac cgtaaaacgg
     3961 ttttttctcc tgtacggatt ccccgttttt tcagacgacc ttccatatca aatacaccca
     4021 ttaaaaggaa tacccatgaa actcctcttc atcctcctag tcctcttcgt cgccgtcgaa
     4081 catttctaca tcgcctggct tgaaatgaca cagattccca gcgaaaaagc ggcggaaacg
     4141 ttcaagctgc cttatgaatt tatggaacaa aatcgcgtgc agaccctgtt cggcaaccaa
     4201 gggctgtaca acggctttct cggcatcggg ctggtgtggt cgcggtttgc cgcgccggat
     4261 aacgcggtgt acggcgcgac ggtactgttt ctcggcttcg tcctgattgc cgccgcgtgg
     4321 ggcgcgttct cttccggcaa caaaggcata ctcgtcaaac aaggtttgcc cgcatttttg
     4381 gcagcggcgg cggtgttggc ggtatgaaaa aaatcaatgt tgcccccgaa aatccccaat
     4441 accgcatcgt cgaaatcttt gaaagcctgc aaggcgaagg ctggaacacg ggcatacccg
     4501 ccgttttcgt ccgcttgggc aaatgcaacc tcgcctgcgg ctggtgtgat accgattatt
     4561 tgacattcgg catgatgagc ctgtccgaca tcttaggctg tctgaaaacc tatgccgccc
     4621 gcaacatcat catcaccggc ggcgaaccga ccatccagcc gcatctcgat atgctgctgg
     4681 acatgctcaa ggcggaaggt tatttcctct gtatcgaaac caacggactc aatcccgcgc
     4741 cgccgcaaat cgactacgtc gccaccagcc ccaaagcctg ctacgccgcc aaatatgaaa
     4801 atagctgtat cgaaacagcc gacgaagtgc ggattgttgc cgatggtgat gtccttgcgt
     4861 tctgcgaaaa catggaacgt aaaatccgcg cgcatcatta ctacctttcg ccctgcgaac
     4921 aagacggtgc gatgaacatc tacgacacca tccgccaaat cggtatttta aacagtcgcc
     4981 ccgacgcgcc ggtgcattgg cagttgagcg tgcagacgca caaatgggcg ggaatagagt
     5041 agtttaagca gtgtaactca aagggccggc gtaccgtttt gccgatgtct aacatatggg
     5101 aaaagtgtgc cgcttccgcg tgaaactgcc cacatttccg ctgcctgatt gaggcggaac
     5161 cttactgaat aagatgccgc cgttgggtac aagcccggct tcctaaattc cgatggtctt
     5221 ttgaaccttg ccgatactct gtgccagtgc gcgcaaatgg cagggttagg gaaaacgaaa
     5281 tgccgtctga aacagcattc tgtttcagac ggcatttttc tgttgacgcc aaaagggaaa
     5341 aaccgcccca gcaatccatg ccgaggcggt ttgaatatgg tcggaatgag aggattcgaa
     5401 cctccgaccc cttcgtcccg aacgaagtgc gctaccgggc tgcgctacat tccgaattaa
     5461 gtaaggcgtg attatagcgc aaaaaatacg gcgtgcctat accgttttgc ctttttgccg
     5521 cgtgtcgggc ggatttaaag gagtgtgttt gaatacagtg ttgataatca tcattatctt
     5581 taagtaattc aataagataa ctttctacct gaccgaaaaa atcattgcct ttccctgaca
     5641 aacggttgat gaaatcggca gattgttgaa acgcagccgg tttaaaaggc ttcgccgact
     5701 tttacgccgc ccgctgtatc ctgcggcgag gcaaggccgg caacaaaggc ttgcgcctct
     5761 tggaaatccg ccgtctgcat aacggcttgc gcggcggcac tgccgagcgt gttggccata
     5821 tattgccaac gttgcgccaa agcgggattg tcaggaatgc ggaaatcttc gcgcagttcg
     5881 tccacaaggt cgggacggtt gcagacgagg acgatgtcgc aacctgcctc aaaggaaatg
     5941 cgggcgcgtt ctttgaggcc gccgacaccg cacgcgccct ccatagtcaa atcgtccgag
     6001 aaaatcacgc ctttgaatcc gatatcgcgg cgcaaaattt gtttgagcca gatttcggaa
     6061 aaccctgcgg gctttgtgtc cacttgtgga taaacgacgt gggcgggcat aaccgccgcc
     6121 ataccttcgc ggctcataat gtggaagggg gcgaggtcgg cggcttcgag ttcggacagg
     6181 ctgcgcctgt cttccggcaa gaccagatgg ctgtctcctt cgacaaatcc gtgtccggga
     6241 aaatgtttgc cgcaggattt cataccgcct tttgccaaac ctttttgaag ggcgagggcg
     6301 aggtgggcga ccgcttcggg attgtggtgg aaactgcggt tgccgatgac ggggcagttt
     6361 ccccagtcca aatctaagac gggcgtgaag gacaaatcga tgccgcaggc ggaaagctcg
     6421 gttgccaaaa cccggccgac ttgtccggcg gcggtttcgg cggcggacgc gccgtctttg
     6481 tcccaaatct cgccgagcgt actcattgcg ggcaggcggg tgaagccttc gatgaaacgt
     6541 tgcaccctgc cgccttcgtg atcgacggcg ataatgagtt cgggtgtgcg cagggctttg
     6601 atttcggcgg tgagtgtttt gagttgttcg atgttttgga agttgcggcg gaagaggatg
     6661 atgccgccta cggcgggatc gagcaggcgt tgcttttcct cttcggtcag gcggaaggcg
     6721 gcaatgtctg ccatgacggg gccgcgcgga atatggggga cggtcattgc ggtttgctcc
     6781 aaaaagcttc agacggcata tgccgtctga acagggaaag gggtcaggcg ttggcgcgtt
     6841 ttttatcttt caacagaaaa atcagcaccg ccaatacaat gcctgtcgtg ccaaagccca
     6901 acagcgcgga ttttgtcaga cccaatgcga ggtagcccga tgcggcggcg gcggcaacgg
     6961 ttaaggcgta aggcagttgc gaggtaacgt ggtcgatgtg gttgcagcgc gcgccggtgg
     7021 acgacaggat ggtcgtgtcg gaaatgggcg agcagtggtc gccgcatacc gcccccgcca
     7081 ttactgcgga catacacggg ataatcagcg cgggttcgac tttgaccgcc atggcggcgg
     7141 caatcggcag catgatgccg aacgtccccc agcttgtgcc tgtggcaaac gccatcacgc
     7201 tggcgagcag gaaaaggatg acgggcagga agccgggatg gatgttgccc gcaaccagcg
     7261 tggagaggta gtcgcccgtg tgcatttcgc cgacaaccgt actgatgagc caggcaagga
     7321 ttaaaatggc gattgcgccg aacatggatt tcgcaccctg ccaaacggct ttgggataat
     7381 cggcggtttt aatcgtgccg agcgtgcaga ggacgacggc aagcacgccg caagtgccgc
     7441 cgaataccag cgaagtgttc acgtccgtat tttcaaatgc acccaaaatg ctgaaggttt
     7501 cgcttgcctg tgcaccggtg tagatcatgg cggaaaccgt tgaggcgatt aaggccaaaa
     7561 cgggaataat cagtgcgtaa acacgacctt tggtagcgtc tgaagcggca gtttcatcgt
     7621 gggcttcgtt caacgcggct tgttcgaaac gcgccatcga gccgatgtcg aaggagaacc
     7681 atgcgacgac gaacaccata atcagggcaa acagtgcgta atagttcatc aggctcatgg
     7741 cgacaaacgt ccccatcggc gtgtattcgg tgattttgta ggtaacgagc agtccggcaa
     7801 gcgtggcgat aatcgacgcg ccccagcttg aaacgggcat cagcacgcac ataggcgcgg
     7861 cagtggagtc gaggatgtag gcgagtttgg cgcgggaaac tttaaacttg tcggtaacgg
     7921 ggcgggcaat cgcaccgacg gcgagactgt ggaaatagtc gtcgataaag gttacgaaca
     7981 cgaggcaggc ggtcagcatt ttcgcgccgc gccggttttt aatgtgccgt tttgcccagt
     8041 cggcaaacgc ctgattgctg ccggagtagg tcagcaggga agtaaaaata cccaaaagta
     8101 tcaggaaaac caagattttt ggtttgccca gcgaccaatc gccgtctgac caagccaagc
     8161 cgacgaccat gtctttcagg tgtgtcagac cgtcgacggg gttgccgccg accaaaaagg
     8221 caacgccgac cagaataccg atgcctaaag acagcagtac gcggcgggta atgacggcaa
     8281 gtgccagtgc caaaaagggt ggcacaaccg agaaaaatga atgtgaatag tcgatcagct
     8341 gcatggttta cgggggtgtt aagcgtccgg atgggagcgt atctgtccgc ctccggtttg
     8401 ggttttgttg gcaaaatggg cggaaatatt ttttgtcgta aaaaatattt gtttaaaatc
     8461 aaccaactga tttctgtaaa atgcccgtta atcggtattg acgggcattt tatcatttaa
     8521 aaaatatttt ggttaaatta tgtgtgttat tgcaggttta atgcgatgaa cagcgtgttg
     8581 ccacggcgca tgaccagcag ggggacgttt ttgcctgcct tgtccatagc tttgcggaaa
     8641 ccgtcttcgt cattgacggg gacttgcccg acggcaagga tttcgtcgcc gcgcctcaag
     8701 cctgcgcgtt ctgccgcccc cgaaacccgc acgacgacaa gccgtccgcc gctgctgtcg
     8761 gtatgtgtct gaagggtaat gcctgcggat tcgaccgaga acgtaccgga ttggtgttcg
     8821 gcataagggg ctttgtccgg ctcggacgag gaaccggttt gttcggaagc attgcccagt
     8881 ttgacggcaa cggtgatttc cttacctttg cgccatacgc cgaggctgac ttcttttccc
     8941 ggcgtaatgg cgccgaccat aacgggaagg tcgccggaag aacgtatttc tccgccgtcg
     9001 aggctgagga cgatgtcgcc cgcctgcagg ccggcacgtt ctgcggggct gccgggcagg
     9061 attttggcaa tcagtgcgcc gccggctttg tccaaaccga acgattgtgc caaaccgtag
     9121 gatacttctt gaataatcac gcccagttgt ccgcgttgga ctttgccggt gtttttcagc
     9181 tgttcggcga cattcatggc aacgtcaatc gggatggcga aggaaatgcc catgaatccg
     9241 ccgctgcggc tgtatatttg cgagttgatg ccgacgacct gtcctttcag gttgaacagc
     9301 gggccgccgg agttgcccgg attgatggca acgtcggttt ggatgaaggg tgtgtagctt
     9361 tcgttgggca ggcttctgcc tttggcggac acgatgccgg cggtcacgct gttgtcgaag
     9421 ccgaagggcg cgccgatggc ggcgacccat tcgcccggtt tcaaatcttt gggattgccg
     9481 attttgacga cgggcagctc ttccgttgcg tcgattttca gaagggcgac atcggattgg
     9541 acatccgaac cgatgagttt ggcggtatat tcgcgcttgt cgttgagcag gactttgata
     9601 ctgcccatgc cggtaacgac gtgcgtattg gtcagaatat agccgtcttt gctgatgatg
     9661 aagcccgaac cgaagttcaa tccgccgtca tctgcttctt cttgggggat ttcgggcata
     9721 ttcgggacga ggcgtttgaa aaattcgtag aacgggtcgc tgtcggcaag cgggtcggaa
     9781 tcggtttcgg cattgccgct gccgttttgg gtgcgcgggg cgggggctgc ctgaatattg
     9841 acgactgccg ggccttcgct ttgaaccagt tggacaaagt cgggcagcag catactgacg
     9901 ctgccgtcgt ctttggtgtg ttcgatgcgt tctacgaagg atgcttcttt tttgtccgca
     9961 ccgaaaaagc tgccggcttt gtcgcagcct gccagcgagg cggcacacag tgctgccaaa
    10021 gcgaggtatt ggtatttttt gaacacgttt tgtcctttgt cggatgccgg taccggcttt
    10081 aatgccgtct gaagcgcatt ttgtcggctt cagacggcat aggttgaaat tctacaacgt
    10141 ccgtccgaat tttcaagcgt ttcattttga agggcggcgg cggtcaggct ttggcgggat
    10201 attcgcacaa atcgttgatg atgcaggttt ggcattgcgg tttgagtgcc ttgcaggtgt
    10261 agcgtccgtg caaaatcagc cagtggtgcg cgtccatcag aaattcttta ggaatgaagc
    10321 gcatcagttt gtcttcgact tcgcgcacat ctttcccggg ggcgattttg gttcggttgg
    10381 acacgcggaa aatatgcgta tcgaccgcca taacggggtg tccgaacgct gtgttcaata
    10441 ccacgtttgc cgttttgcgc cccacgcccg gcaacgattc caaagcctcg cggtcttccg
    10501 gcacttcgcc attgtatttt tccagcagga tgcggcaggt ctgcatgatg tgcttggact
    10561 tggttttata caacccgatg gttttcgtat attccatcac gccgtccaaa cccaaatcca
    10621 gcatcgcctg cggcgtatcg gcaaccggaa acagcttcgc cgtcgctttg tttacgccga
    10681 cgtcggttgc ctgtgccgac agtaaaacgg cgattaaaag ctcgaaaggg gagttgaagt
    10741 tgagttctgt ggtcggatgg gggttggcgg cgcggaagcg ttcgaagatt tcttggcgga
    10801 tgtgtctgtt cattttttta tacggtgggt ttgtgtgttc ggcattataa cgtatggttc
    10861 aggcggcgta atattgcatt ccccacagaa tgaaggcgta acgcgccgtt ttgccgataa
    10921 ccagcatcag cccgcttgtc cacggattca accgcagcca gccggcggca agcggcagtg
    10981 cgtcgccgac gacgggcagc caggcaaacg gaagcagcca aataccgaaa cgccgcatca
    11041 gattcagtgt tttttcagac ggcatttttc gggagggcag caaacgcccc atccaatagg
    11101 aaaccatact gcccaatccg ttggcaaggc cggcgcacag caacgcgccg tatgcgtgtt
    11161 cgggaaagcg gtggacgaac agggcaaagg cggcttcgga tgtgccgggc aggagggtgg
    11221 cggaagtgaa ggcggaaaag gcgagggcgg cgtaggtgta ggagggtatc attgcaaaca
    11281 gtctcaaaca ggtaacaatc ggcgacggat tg
//
LOCUS       DAWGJV010000059        11150 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWGJV010000059 DAWGJV010000000
VERSION     DAWGJV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.059"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            39..462
                     /locus_tag="WHQ94_001117"
                     /pseudo
     CDS             39..462
                     /locus_tag="WHQ94_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="WHQ94_001118"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="WHQ94_001118"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ2128408.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1211..1286
                     /locus_tag="WHQ94_001119"
     tRNA            1211..1286
                     /locus_tag="WHQ94_001119"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Gly,seq:gcc)
     gene            1312..1387
                     /locus_tag="WHQ94_001120"
     tRNA            1312..1387
                     /locus_tag="WHQ94_001120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1345..1347,aa:Gly,seq:gcc)
     gene            1411..1486
                     /locus_tag="WHQ94_001121"
     tRNA            1411..1486
                     /locus_tag="WHQ94_001121"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Gly,seq:gcc)
     gene            1508..1583
                     /locus_tag="WHQ94_001122"
     tRNA            1508..1583
                     /locus_tag="WHQ94_001122"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Gly,seq:gcc)
     gene            1610..1683
                     /locus_tag="WHQ94_001123"
     tRNA            1610..1683
                     /locus_tag="WHQ94_001123"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Cys,seq:gca)
     gene            1786..1875
                     /locus_tag="WHQ94_001124"
     tRNA            1786..1875
                     /locus_tag="WHQ94_001124"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1820..1822,aa:Leu,seq:taa)
     gene            1979..3031
                     /gene="sohB"
                     /locus_tag="WHQ94_001125"
     CDS             1979..3031
                     /gene="sohB"
                     /locus_tag="WHQ94_001125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ2128409.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3082..3948)
                     /locus_tag="WHQ94_001126"
     CDS             complement(3082..3948)
                     /locus_tag="WHQ94_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ2128410.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGGVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLECDNHFIRKGDRRQLDRHYGLVARLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(4035..4904)
                     /locus_tag="WHQ94_001127"
     CDS             complement(4035..4904)
                     /locus_tag="WHQ94_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ2128411.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(4999..5916)
                     /gene="hisB"
                     /locus_tag="WHQ94_001128"
     CDS             complement(4999..5916)
                     /gene="hisB"
                     /locus_tag="WHQ94_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ2128412.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(5913..7010)
                     /locus_tag="WHQ94_001129"
     CDS             complement(5913..7010)
                     /locus_tag="WHQ94_001129"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ2128413.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFSSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7056..8345)
                     /gene="hisD"
                     /locus_tag="WHQ94_001130"
     CDS             complement(7056..8345)
                     /gene="hisD"
                     /locus_tag="WHQ94_001130"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ2128414.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8342..9241)
                     /locus_tag="WHQ94_001131"
     CDS             complement(8342..9241)
                     /locus_tag="WHQ94_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ2128415.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9345..9998)
                     /locus_tag="WHQ94_001132"
     CDS             complement(9345..9998)
                     /locus_tag="WHQ94_001132"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ2128416.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10077..10730)
                     /locus_tag="WHQ94_001133"
     CDS             complement(10077..10730)
                     /locus_tag="WHQ94_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ2128417.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11007..11091
                     /locus_tag="WHQ94_001134"
     tRNA            11007..11091
                     /locus_tag="WHQ94_001134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11041..11043,aa:Leu,seq:caa)
BASE COUNT         2474 a         2656 c         3159 g         2861 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgcccccaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac ggtttgtgcg cggaaggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct ataagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttca gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccccatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcatgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttaaccgac
      721 tggtttgacg gatttttggc aagactgtgg aaacagactt ccgatttcgg cgccttcctc
      781 gatcccgtcg ccgacaagct gatggttgcc gtatcgctac ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatt atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 tttaaaaccg ccgcgcaaat gctggcaatt ttcctgctgc tgctgaatat tcccgatttt
     1021 tacggattta atcttgttgt tatcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg cgtctcttcg atgttggaga
     1201 atgaaatcgg gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc
     1261 gagttcgaga ctcgtttccc gctccaaaaa tttcattttc ccacaatcat tgcgggaata
     1321 gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag actcgttttc
     1381 cgctccaagt tttatttcaa acatatcaac gcgagaatag ctcagttggt agagcgcaac
     1441 cttgccaagg ttgaggtcgc gagttcgaga ctcgttttcc gctccaaaaa ccgatatgtt
     1501 tgaacgcgcg ggaatagctc agttggtaga gcgcaacctt gccaaggttg aggtcgcgag
     1561 ttcgagactc gtttcccgct ccaagttttt ttagataagc cagttttagg gcgagatagc
     1621 aaagtggtta tgcagcggat tgcaaatccg tctacgccgg ttcgattccg actctcgcct
     1681 ccattatcgt actatggcgg ggtggcagag tgtttatgct atgaggagtg caatcttcat
     1741 ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa
     1801 taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc ggttcgattc
     1861 cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg
     1921 tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa tccaatcaat
     1981 gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg
     2041 cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag
     2101 tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt ttgaagcatt
     2161 ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa aggaaaaggc
     2221 ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctt ccgaaacgca
     2281 aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc
     2341 cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct
     2401 tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt cacagcttag
     2461 gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg cagcgagcgg
     2521 tggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattgtcgg
     2581 ttcggtgggt gtggtggcgg aagtaccgaa tatccaccgc ctgttgaaaa aacatgatat
     2641 tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa
     2701 tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc agttgttcaa
     2761 gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa cgggcgagca
     2821 ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga
     2881 tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagca
     2941 aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt tgtttgccaa
     3001 acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga
     3061 agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat
     3121 ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca cgtcgtccaa
     3181 actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg cgctgctgta
     3241 gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac
     3301 gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg caaccagtcc
     3361 gtaatggcgg tccagctgcc gccggtctcc ttttcggata aagtggttgt cgcattccaa
     3421 gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca
     3481 taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag
     3541 catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc
     3601 aaaaaagctg acaaactcac gcgacagggc aacccgcgca gttaccgccg aatgcgggac
     3661 ttgtcgctgc cgctgttcca acacgaccgg cacggcgatt tcggtataaa tccgcgtaaa
     3721 cgtgcgcgta tcggcaatcc gccgcgccgc accgaccagc cacatcaaaa gcacaaccaa
     3781 agcgtacagc aacgcctgcc acaccccccc cgcaatcacg gcattgatcg cccggccgcc
     3841 aaacaccgga tacaccagca tcaaaaggtt ttccagtccg accagggaaa atgtgccaat
     3901 caatcgcttg cggtgggttt gggctatgtg tttcaacatt ttccacatat ttgttcccta
     3961 ttttttattt agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga cggcattgtg
     4021 tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac gtcctgttcg
     4081 ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac ggcgggcagg
     4141 gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga ggcgtgtttg
     4201 agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt ttggaacatg
     4261 ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc gatgccgaac
     4321 tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa gagcgagttc
     4381 aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt ttttttgccg
     4441 acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc gaacagaatc
     4501 agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc ggcaaactgt
     4561 ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga gatggtgctc
     4621 atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat gtcgcacacg
     4681 gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag ttcggcggtg
     4741 ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg cgagcggttg
     4801 tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag acccatttgc
     4861 cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt tactcctttt
     4921 ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc ctgcgggtgt
     4981 ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg tctgtcctgc
     5041 catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt tgaatacggt
     5101 ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg tcatcatact
     5161 gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac gtccgattag
     5221 tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt cgatgacgac
     5281 gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga tgcctttttt
     5341 gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt cggcggtgtg
     5401 gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca tgccgtggcg
     5461 ggcgatttgg tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc tgcttttgcc
     5521 gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc ggttgacggt
     5581 aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg cagttccggt
     5641 gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga gccagccttt
     5701 gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc cgcgtgcatc
     5761 tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga cgggggtacg
     5821 gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga gggtcagaaa
     5881 gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg ttggcagtaa
     5941 agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac ggtaatgcgc
     6001 aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat gcggttttgt
     6061 ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa gtttgcctga
     6121 cttgaaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg ttcgcgttcg
     6181 tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc gagtttggcg
     6241 gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag ttcgccgatg
     6301 acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat tttgctgacg
     6361 gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct gtcgccgttg
     6421 aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat gacggcttcg
     6481 atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat aaaggtcagg
     6541 gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt gaaatctccg
     6601 ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg gtacatgacg
     6661 aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat cgtgatgaac
     6721 tgtatcagct cgtccgagcc gttgcccagc gcgatgtcgg cgcagtcggg aatgtcgaac
     6781 gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag atggatgggc
     6841 gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc aaaagggtgg
     6901 acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc aatctgatat
     6961 gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat cgtgtttcct
     7021 taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa ctctgccgcg
     7081 cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc gcctaatttt
     7141 tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc atatgtcccc
     7201 aaaggcgaag aaaagcgggc agtgcggctg gtgggcaata catggtttgg accggcgcag
     7261 tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc gtggcggatt
     7321 tttttcgccc attcctgcgg attttcgact gacaattcca agtgttcggg ggaaatgtag
     7381 ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc cctgttgccg
     7441 agcgaggctt cgatgatgtc gcggcgcggc atggtttcga tgagcctgtc catagcggct
     7501 tctacttcgt cgagatacgc ttgcgacgtg ccgattaaga tggcttgggc aatttcgtcg
     7561 tgttcggcct ggctgaacaa atccatcgcc acccaatcgg caggtgtcgt gccgtcggcg
     7621 atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc gaacacgcgg
     7681 cgtttggcgg cggcgacgaa ggcgttgccc ggaccggtga ttttatcgac ttgggggatg
     7741 gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt gaagactttg
     7801 gttacgccgg cgacgtatgc ggcggcaagc acgatgtcgt tgcgttcgcc tttcggtgtc
     7861 ggcacgacca tgatgatttc tttcacgcct gcgacgtggg cgggcatggc gttcatgatg
     7921 acggaactcg gatatgccgc cttgccgccg gggacgtaaa tgccgacgcg gtcaagcggg
     7981 gtaatctgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga ttccattttt
     8041 tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt ctgaacgtcg
     8101 ttcggaatgc gctcgaacgc cgcgttcaaa tcggcttgcg tgagtattaa atcatcgatg
     8161 cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc atcgccgcgc
     8221 ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt ttgcgcggtt
     8281 tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt gttgagtttt
     8341 ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga atattctctt
     8401 gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg ctaccaaact
     8461 tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct tcgctacgag
     8521 aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc tcaaccattg
     8581 tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg gtgtgaacgc
     8641 cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg cccaacgtac
     8701 gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct tcgtcagtat
     8761 ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg ttaatctcgt
     8821 taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta ataccacccg
     8881 gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta acaaaatgaa
     8941 ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg atgatatagc
     9001 gaccgagttt cttgcatttg gagatggctt tgagttttcg atcgataatc tcgccggaga
     9061 ctgggtcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta ccgcagcctt
     9121 tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca taaaaccgac
     9181 gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt tttgcaacca
     9241 ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct gtcggcggga
     9301 acggataggg tcgtctgaaa acgcatcttt attcaaatgg atgcttactt cgccttcact
     9361 gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt caaagcagcc
     9421 ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc caagccgttt
     9481 gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa gccgaccagc
     9541 ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc cttgccggca
     9601 aaatgagatg ccgcgatttc aggatacttc gtggcaatct tcagacggca tccgggttgc
     9661 gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca cttggcaatc
     9721 tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac gtctttgccc
     9781 gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt tgcgcggaca
     9841 atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga tttttcaggc
     9901 tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc aaaaatgcgc
     9961 cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc ctttgtcggt
    10021 tgaaacaata atgccgtctg aataggcgcc gacggtgcag acggcatacg gttttatcag
    10081 agcagagtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg cgagaaaatg
    10141 gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac caaatagttt
    10201 tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg cttgatgacc
    10261 ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa ctgtttggca
    10321 tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa caccactttc
    10381 acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa aagctctgtc
    10441 gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt gcctttcaaa
    10501 tcgctcaggc tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc gccgatgtct
    10561 tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc agctgacgcg
    10621 gatgcacttg aagaagcggc ttgcgccgca ctgttgtctt gaggtttgca ggcggcaagc
    10681 gcggcaagta cgaaaacgcc cggcaaaaag gaacgcggta cggaaaacat gacaatctcc
    10741 caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc ttaacgcgtt
    10801 tggcagattt aatgtagtat ttttttacgt tgtttgcacg gcaggaattg ccgaatcata
    10861 cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct atttaaaatc
    10921 aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga atttcaaaag
    10981 ccattataat gacgacttct tcaccagcct aggtggcgaa attggtagac gcaggggact
    11041 caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc acaaccgctt
    11101 ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataaaac
//
LOCUS       DAWGJV010000060        11112 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWGJV010000060 DAWGJV010000000
VERSION     DAWGJV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.060"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(125..337)
                     /locus_tag="WHQ94_001135"
     CDS             complement(125..337)
                     /locus_tag="WHQ94_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128418.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(349..630)
                     /locus_tag="WHQ94_001136"
     CDS             complement(349..630)
                     /locus_tag="WHQ94_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128419.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(677..940)
                     /locus_tag="WHQ94_001137"
     CDS             complement(677..940)
                     /locus_tag="WHQ94_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128420.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(960..1532)
                     /locus_tag="WHQ94_001138"
     CDS             complement(960..1532)
                     /locus_tag="WHQ94_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ2128421.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1511..1822
                     /locus_tag="WHQ94_001139"
     CDS             1511..1822
                     /locus_tag="WHQ94_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128422.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1844..2719)
                     /locus_tag="WHQ94_001140"
     CDS             complement(1844..2719)
                     /locus_tag="WHQ94_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ2128423.1"
                     /translation="MSNEVEVMDAVSVQNYQVAHSVMVMEQWGNGEVYNEATWIERGR
                     LAVRQTMEGMFELGRALIVLKEHTEHGRFMEIVKGQFGIGHNETARLMSATQRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2765..3055)
                     /locus_tag="WHQ94_001141"
     CDS             complement(2765..3055)
                     /locus_tag="WHQ94_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2128424.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRRQHERLQSKIAEIGKRVNAD"
     gene            complement(3067..3312)
                     /locus_tag="WHQ94_001142"
     CDS             complement(3067..3312)
                     /locus_tag="WHQ94_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2128425.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAQNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGYEIAKKLGLK"
     gene            complement(3305..3529)
                     /locus_tag="WHQ94_001143"
     CDS             complement(3305..3529)
                     /locus_tag="WHQ94_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2128426.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3801..4172
                     /locus_tag="WHQ94_001144"
     CDS             3801..4172
                     /locus_tag="WHQ94_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128427.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLALFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4174..4797
                     /locus_tag="WHQ94_001145"
     CDS             4174..4797
                     /locus_tag="WHQ94_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128428.1"
                     /translation="MNQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4790..4984
                     /locus_tag="WHQ94_001146"
     CDS             4790..4984
                     /locus_tag="WHQ94_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128429.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5008..5568
                     /locus_tag="WHQ94_001147"
     CDS             5008..5568
                     /locus_tag="WHQ94_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128430.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAMPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5709..6767
                     /locus_tag="WHQ94_001148"
     CDS             5709..6767
                     /locus_tag="WHQ94_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128431.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SIPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            6757..7263
                     /locus_tag="WHQ94_001149"
     CDS             6757..7263
                     /locus_tag="WHQ94_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128432.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            7367..7870
                     /locus_tag="WHQ94_001150"
     CDS             7367..7870
                     /locus_tag="WHQ94_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ2128433.1"
                     /translation="MSTKFNQFINRVLSHEGGYVSHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7982..8314
                     /locus_tag="WHQ94_001151"
                     /pseudo
     CDS             7982..8314
                     /locus_tag="WHQ94_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8311..8721
                     /locus_tag="WHQ94_001152"
     CDS             8311..8721
                     /locus_tag="WHQ94_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ2128434.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMAALKKLDALIYGKEVRQKERNSSQG"
     gene            8726..9475
                     /locus_tag="WHQ94_001153"
     CDS             8726..9475
                     /locus_tag="WHQ94_001153"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ2128435.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLADEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(9794..10156)
                     /gene="ssrA"
                     /locus_tag="WHQ94_001154"
     tmRNA           complement(9794..10156)
                     /gene="ssrA"
                     /locus_tag="WHQ94_001154"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10219..10823)
                     /locus_tag="WHQ94_001155"
                     /pseudo
     CDS             complement(10219..10823)
                     /locus_tag="WHQ94_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
BASE COUNT         2753 a         2524 c         2757 g         3078 t
ORIGIN      
        1 agtgagttta ttttggggcg gcggcaggtc ggggcaagcg gcgtggggct tggttgtggt
       61 ttttaggttt gggggtgggt aaaaaatgcc gtacccggcg ggcaggcttc gcactctgaa
      121 ggtttcagac ggcgtttgtt ttttctgtcc aatcgaggaa ctgccgccat ttttccagcg
      181 gcatatcggc ccgccgcgtt tcggtatcgg gttcggcttc ccagcggcct acctgtatgt
      241 agtgcttcac cccgacgatt tgcgccaact cggcctgtgt cagtttgcag cggttgcgca
      301 gggtgcgaag gttgtaaggc gtgtagccga gttccatgtc gttgcggttt attttgtttc
      361 gcatattttt ttgactgccc ggcggcaggt ttcggtaagg acggcggcaa atcgggcttc
      421 gtctgccggt tttccgtcga aaaaaataaa atcgtaaagg gtgatgccgt tttcggaatg
      481 gcggtaatgc ccgcccgggc attcgctgtc tgtctgcctg tggattttgg caatcaggcg
      541 gggatagcgg caatgggtaa tgaaacggct tgcgccgtct tgcccgataa tccattcggg
      601 gtagcggttg aatagtgcgg ctctgttcat tttgttcggg ttgttcgggg gataaagccc
      661 ctcgcggggc ttgtggtcag gcaaatttga atatcagtgc caacacggcg gcgatggcgg
      721 taaccagccc ggtggccgcc atcatgggat accatcggga ttcttgggct atttttacgg
      781 attcggcgtt gattttgtgc gcgtctgcaa tgattttggc gatttcggca tctacttttc
      841 tcagactgga gtgtttcatt tcttgttcga ttatgttcat cgtacttcct ttcgtttttg
      901 gcggttgccg ccgcttgtcg gatggtagga tgtctgccat gtgtatatat tgataccttt
      961 taggttttat tgcaagtgtt ttgggcggcg gcttcgtatg cttggcggtg gcggcggctg
     1021 taccattcgg cgagttcgcg ccgctcttgg aggcggtagc tgtcggcggt caggtagtgg
     1081 tgcaggcttg agaagccggc gcgttggagg gcggcgcggc tgatgtggcc gagggcgagg
     1141 tctgttgtga gggtgtcttt tccggcggtc aggctgatgt tggtgaagag gtggcggaat
     1201 ccgtgcattg tgtgtttgga tttgccgggg gtgctgccgt catagcccag tcgtcggatg
     1261 gcgttgtggg cgaatttgat gctgatgtgg tcgggatggg gggcgggttt gcgccgtggg
     1321 cggatgccgg ggaaaaggtg gatgttgtcg ccggtctgtg tgtgcagctc tcggagtatt
     1381 tctaccgccc agtccgacag tgggacggta aaggggtgtt tggtcttcat gtcggcggcg
     1441 gggatgtgcc ataaccgggc ggtgaggtcg atgtcttgcc agcgggcgga aagcagtgcg
     1501 gagggacggg atgacttccc cggtttcccc atcaatccgc gccgcctcga tttttccgtt
     1561 ggcgcgcagc atttccaccg cctgccgcac cgacatcaac cgcttgccgt tgcgcctcat
     1621 cgcctccacc aaaaccgccg aaatcaattt ggcttcttcc ggtttaagct ccgtcttgcc
     1681 cgcatcgctg cgccgtttgc gcgtcggctt gacgctgacc gcctccagct tgcggtatag
     1741 cgtggcaagg ctgatgccca attcctgcgc ctgctgctta agatatgcag agcgtgcgcc
     1801 gcgttccatt gcttccgcct gattctcgac tgccttaaga cgctcaatca ttgccggatt
     1861 catcgccttc tcccgtttca ccgcccaacc attccggcac attgtctgtc ggtgcttcag
     1921 tcggcagggc atagctttcg cgcagttgct cgcagtccaa aataatttga ttgagcgtgc
     1981 cgaccatctt tgcctgatgg ctgatctcgt gtgcctcact gtgcgcatta agttggtcga
     2041 acaaatcttt caaacggctc acttgactgc ggataccgac ctcaaggctt gttaactgca
     2101 tcgccaactc actgcccacg tcttccgcct tcggctctct gacaacggtt tgcttcttag
     2161 ccagcttctc ggtcagctca tcaattttgg ctgttttggt tttcatcact tcgtctttgg
     2221 cggcgaggtt ttcacggctt tcgcgcaggg cgacgcgcag ctcgcgcacc gtcattcggt
     2281 ccacatcgtc aaaggtcatg ccgttgactt cttctccttc ggcaagtccc accaacgtaa
     2341 cgtcttcttc gaccaagagt tccagcagct ttgacttgcc caaatccatc agcttcggcg
     2401 cggctttctg catttgcggc gtggcgaaac gttgggtagc agacatcaat cgggcggttt
     2461 cgttatggcc gataccaaat tgacctttta caatttccat aaatcgcccg tgttctgtat
     2521 gctctttcag tacaataagt gcgcgcccca attcaaacat gccttccatc gtctgccgta
     2581 ccgccaagcg gccgcgttcg atccaagtgg cttcgttgta aacctcgccg ttaccccatt
     2641 gctccatcac catcacgctg tgcgcgactt ggtagttttg cactgaaacc gcatccatca
     2701 cttcaacttc attgctcatt tttcactctc caaatttcct tacgtcagga aatttcaaaa
     2761 ttcgttaatc tgcattcacc cgcttgccga tttcggcaat cttgctttgc agccgttcat
     2821 gctgccgcct gaaccgctcg gcgatttgca gggttttgat accgtaggcg tagttgccgt
     2881 tttcaagctt gatgaccaat cccgatgcaa ccaaatcatc aatatccctg ctgacttgtg
     2941 atggcgtcag ccccagtccg accgataaat ccttattgct cagaccgata atcggatgtt
     3001 cgtcaagcgc gataaagacc ctcaatagcc gttgtaccct tttactttct gccatccgca
     3061 tcctccttat ttcagtccca gcttcttggc aatttcgtac cccttgccgt aattgccttt
     3121 gcgctgtccg ccgatcacta gatacacatc gcgcggctta aagccgttct ctcttgccca
     3181 ctgcgccagc gtctggccgt tttgggcaaa attttctttc aattcgtctg ccgtaagcat
     3241 ttctttggcc tgtacgcggg aaaatttcag tatccgtccg tacggttggt taatacactc
     3301 atgattaatc atcgcttttc tccttcatcc ccagcaaaac cgccgcttcg tggcttttgc
     3361 caaaattgcc tttcagcttg ccgcgcaaga ggtgctccac cgtggtgcgc tctagattga
     3421 aatatttcgc ccaatgcgcc ttgcacaccc cgttgcgctt aaaccacccg gccgcgctct
     3481 cgcgcgtttg cggataggga ataggcttga aattcaaagg tttttccata ttatttatgc
     3541 ctttcgtgtg atataatgtg tttgttttta ggtctctttg cacgataaac atccggtctg
     3601 ttgtgcagca gcagggtctt ggcttgtttt aaagtaaaca agctttttct cgtagctggg
     3661 tgtatgtgat ccatcatgag ctgtcgcgca tgagctttca aagtgttcat tttttcgtcc
     3721 tttctcgtga tgatttaggg tgtttgtgtt tcgatgtgga aattatagga agaattcttc
     3781 ctattttgca aggaatattt atgaataact cttccttttt tggcaaccga ttgaaagaag
     3841 aaagaaaaaa attaaaaatg actcaagctg aaatcgctga aaaatgtggg gtttcaggaa
     3901 gaatgtgggg ggattatgaa cgtggcatca gccagccaaa agcggaactt ttcttccaat
     3961 ttgaaaaggt gggtatagac gttcaatacg tcatgcacgg cagacgcggc gaaacagcgg
     4021 tcatgccgtc tgaaaccctg aacgccgaag aacaagaact gcttgccctg ttccgcgagg
     4081 cggcagctgc cgaccgtgaa atgattctga tggttgcgcg cagggcagag aaaaaagccc
     4141 aaactgcgct tggtaaagtg agtaatggat aaaatgaatc aattcgaatt gtgccaaaaa
     4201 gaacatgtca atccatttgc cttgtcaaag caatatctat tggttgtaac attcgtcaaa
     4261 tccagcagta aaaattttca ggcagcatta ctttgggcaa gaagtgccaa attatttgag
     4321 aatcttgaga ttggaaaaga aaccatctat tgctgcgctt tcgataaaac agcagaacag
     4381 gctgggatgg ccggggtatt tttgaattat attgaaaatt ggaatggcaa acagatttac
     4441 atcaatggcc gaatccatag tggcagtatt tatgatttgt taggggtttt agactgctat
     4501 caaaaatcac agtcctgtcc caaccctaaa agccactgtt gctttgtttc agacgacatt
     4561 tttctatggc atggatcaag accaacgttt gaaatcagtc ttgatctaac tggaaagaaa
     4621 aaagaaacat cctctgcaaa gaaatttgtg atgccttgta ttaatttccg tcaccatagg
     4681 attgaaaaag aaacctactt aggaaattgg aatgaacaaa ttgccgcatt ggcagtaaaa
     4741 caaaatatag attggtgtcc aagttttgat attgagaatt ttagacagta tgaataatta
     4801 ctatctatat aggaattgca gcagcgatgt gttatgggtc aaacgtatcc aacgccaaat
     4861 cgacggcagc ctactcttga tttctgacaa ttcaacctat ccacccatgc ccttggcact
     4921 ggcggaacac cccgatattc aaatcatcgg gcaggtagtg caggtatcaa aagacttgaa
     4981 ctagacacaa tcaaaaaggg aaatagaatg aaaatactcg ctttattaat tgccgctacc
     5041 tgtgctttat ctgcgtgtgg cggccaagct gaagaacaac cggcatctgc ccaacctcaa
     5101 gagcaggcac aatccgaatt aaaaaccatg ccggtaagct ataccgacta tcaatcagta
     5161 gccaataaag ggcttgccga ccaaaaaact ggtctgaccc ttccagaaca tgttgttcct
     5221 gccgacaatg cggaagggaa gaatctgctg catgactttt cagacggcct cacattaacc
     5281 gttgataccg ataaagccga caaaattact gctgtccgag tagtctggaa tacagatgca
     5341 atgcctcaaa aagcggaaaa actgtccaaa gctgccgcag ccttgattgc ggcaaccgcc
     5401 ccgggagacc gcacactgct gcgtgatacc ggcgaccaaa tcaaaatggc aattgacagc
     5461 cataatgcgc aaaaagagcc aacccgagaa tgggcgcgtg gtgggattgc ttataaagtc
     5521 actgttacta atttgccgag cgtggttttg acagcaaaag ctgaataacc tgcattacag
     5581 ccacaattag gagcagacgg agatgatgtt tatagagcct gctgagaata aataactgat
     5641 aaaaaagctg tctgaaaagg tttcggatag ttaggatagc tatctatttt taatatatta
     5701 gagagaatat gaaaaataaa ctgactttac gttatttcaa aataactcgt tgcggttatt
     5761 ggtctggttc ttcaaaagaa cctaatcatt atgaggactt attaaaaact ttaactcatt
     5821 tagagacata tgttaaaggt aaaactattg aagagactgg aattagtcgt gatgataaag
     5881 agccaagcac tttcttgctc gatatctgca aaaaaaatgg tctgtggttg cttgcttttt
     5941 ggaatcctgc cgcagaaagc tcttcagatg aaattgtttc tttaaacatg aaaagtaaac
     6001 taggtcaagt tgattctatc ccagtaacag caggaaatgg gaaaactttt ggcttcgtta
     6061 gttattttct tatttggcca gaacataatt tttactcaac gattctttct cctaatgcaa
     6121 ctttagctgg acgaacagag tttgaagagt acatgcttaa tttcttgtgg attgatccta
     6181 attttataga aaagactacc tacgaagaaa cttcaacaga cggaataatt cataccatgg
     6241 agcgaactaa ttttaaattg cctcttaaaa ttaatggaac tcctgtgcca cgttttgcgg
     6301 ggaaactagc taaatctcct tcagaaaaag agtatgtcat tagtaattat gaccaagtaa
     6361 cccaagtgca taatcaatgc acggtaaagg ttacctctgg acaaagttca agtggaattt
     6421 tgaagaaaat gttaccaagc ttttttggtt caacagaatt gtttgctcca ttaaaaacca
     6481 ttcgtgtaaa tcattctgtt tctgctgcat ttgaaaacaa atcaaatctt aaggattgga
     6541 ttgaagactg ggaaaaaaat tcttttgatt tagataaaga tgattgtggc tttaagttaa
     6601 aaaacgatga caagatttat tggattcgag gaaatgtcat caaatgtgaa ttagacgtca
     6661 accttcataa ggaaaatggg atttatccag ctgctaaaat tctagatata atagaccaaa
     6721 ggaaagatta cgttttaaac gaattcaaat taagtgatga ttcttagaaa tattttccta
     6781 atatgctctg cattcatgat tggtgtagcc tggtatttag gtgctaatat ccgatttgaa
     6841 gagcaatggg tattgtatga agccttgaga acaactgcat caattatttt tgcagttgtt
     6901 ggggtttggt tggctattgt ctatcccgaa cgattaaaaa gcccttttag tcaaactttc
     6961 tcctctccgg tatatcggcg tggattcaaa cagctttttt cccctgttat aagttccatt
     7021 tttattttaa gtattgtgtt acttgttggt ttgttggctc ctttgctcaa acatattgag
     7081 gcaataattc aatatatccc actttgtcgg aaaatctcat ttagcctgtt agcaagcttg
     7141 acgatgtggc aaatttatac ggttttatct gtattagtgc ctttgttaaa tattttaagt
     7201 aaaaatgaaa atgactgtca tgcatccaac attattaaca agctaaaggg tgaacagaat
     7261 tagctatgtg atttttagtg taaactgctt ttaactcgta ttaaaaggcc actcttttaa
     7321 tacctcctaa aatccctgta cctatcaata gatacaggga ttttttatgt ccaccaaatt
     7381 caaccaattc atcaaccgcg ttctctccca cgagggcggt tacgtcagcc atcccaaaga
     7441 ccccggcggc gaaaccaatt ggggcatcac taagcgcacc gcccaggcaa acggctacaa
     7501 cggctccatg cgtgccatga cgcgtgaaca ggctatcggc atttaccgaa aggcgttttg
     7561 ggagcgttac cgcgccgacc aaatgccgga agcggtcgcg ttccaatttt ttgatgcctg
     7621 cgtcaaccac ggttacggca atgccgtccg tatgctgcaa cgcgccgcag gcgtggcgga
     7681 cgatggaatc atcggcgaaa tcagcctcaa agccatcaat tccctccccg aaaacgacct
     7741 tttattgcgg ttcaacgccg agcgtctggt cttttatacc aagctcagta cgttcacctc
     7801 tttcggcaag ggctgggtac gccgtgtggc gcaaaacctg attcacgcgt ctgcagataa
     7861 cactgattaa agggagataa accatgtcaa aaaagtcact catcgcccta atgaccgcag
     7921 ccatgcagcc cgatttcagc cacagcgacc taggcattcg ctacgccatg ccgactcagg
     7981 gatgttggac gcaagcccac cgcaagagcg gggtagccgc cgcgaaacgc gcagccaaaa
     8041 aaaagcgtca caaataaccg cctttttccg atggctgggc ggcttggtct ctaatccggc
     8101 cacaggaaaa atcagccata ccaaactatg ggcaaacgtc gccgcagccg ccatgacttg
     8161 gaagttcgtg caggcggcgg acgcgcccga atggctctag tgggcttatg gcgcattggt
     8221 cggcgggtat gcattaatca aacgcggcat cgcggcgatt ccgcagttgg cagaaatcaa
     8281 aaaatccgca aatcaggaag gggggcggca atgattgaat ttgtccgagc caaaaaacgg
     8341 ctgctttggg catttgtgct tttgcttgtg tggacgtgcg gttaccgata cgccgccgac
     8401 aaggccgaag cgaaacaaac cgccctgatt gccacctatc ggcattcttc tatggttgcg
     8461 gcggaacaat acgccttgca gcttaaaaaa gcgcaggacg aaaggcagcg gtggtacgac
     8521 ttttcccaaa aacaaggaag aaagcccgtg aaaaaacagt atccgccgca aacgaaaaaa
     8581 gccggctatc tgaaaaccaa ggaagaactg cttgcggaat tggcttgcct taaagcggaa
     8641 atggctgccc taaaaaagct cgatgcctta atctatggga aagaagtgcg gcagaaagaa
     8701 cgcaactcgt cgcagggtta aggcaatgcc atccgttgaa ctgctgttgg tgattgtcgg
     8761 actgccacgc ggcaccttct attaccaatt ggccgtccaa tcggcagaag acaaatatgc
     8821 cgatttgaaa cggcatatcc atgatattta tcgacgacat aagggaagat acggctaccg
     8881 gaggattgcg gcagccatcc gtcacgcagg aacaccggtc aatcacaaga aagtcagccg
     8941 tctgatggcg aagacggggc tgaaggcagt gatacggcgg cgcaaatacc gctcgttcaa
     9001 aggagaagtc ggcaaaattg cgccgaatat cctgcgacgc tgtttccatg cagaaaagcc
     9061 gaatgagaaa tgggtaacgg acgttaccga gttcaatgta ggcggagaaa agatatacct
     9121 ttctccgatt atggatttgt ttaacgggga aatcgtcagt taccgtattc aaacccgccc
     9181 gactttcgat ttggccgacg agatactgaa aggtgcgccg gagaaaccgg gatcgtctga
     9241 aaagccgata ctgcattcgg atcaaggttg gcaatatcag atgttttttt atagtggatt
     9301 aaatttaaat caggacaagg cgatgaagcc gcagacagta caaatagtac ggcaaggcga
     9361 ggcaacgccg tactggttta aatttaatcc actatacaac aacgatagaa tcaagttgaa
     9421 attaaaaggg ctgagccttg ttcagtacag aattcagtcc ctgaaagccg cttgattaaa
     9481 ctgtccgact ttttggggtc agttcggctt cggcattttt ttatccgttt gggggtaact
     9541 tgtttggaaa gctgcaagct tataaataaa ggattacatt taagttttgg gtaacctttt
     9601 taaaaaaatg cgtgatgact tttgcatttt taaggcgttt tttggggtaa ttcgtgaaaa
     9661 gttaccccaa aagttacccc ataaatggcg aaaactcaag catacgccag catattcaag
     9721 gcaacaaaaa agccttgaaa ctgttgaagt tcaaggcttt tttgtgttgc aggatgctgc
     9781 tgaaaatagg gtatggtgga ggcgggggga atcgaacccc cgtccgaaag tcctctacaa
     9841 agcgttctac atacttagtt gtgtctattt gaaaatctta tttccatcat gccgaccaac
     9901 aggccttttg gaaaccagtt accttaaatc tcatttcctg ccaagtaacc cggtaggaaa
     9961 ccagtcaatg taagatgacg ttgcggtggc tttcgccaca cagcccattg accgactgct
    10021 gcaacggcta gccttaagcg gctaaagcgt aagtttcgtc gtttgcgact atttgaattc
    10081 agtgttttac gggaatctga gaccccggta tgcccgcatc tgcttcgcaa cccccgtcga
    10141 aaccaaggtc gcccccagaa atggtttgca aattatacgg atattgtgcg gtgctgccaa
    10201 gtctgtcgga gaaatttgtc agtcttgctg ccttaatttg cgtttgagca ggatgcggac
    10261 gcagccgtcg ttgccttccc ggggttcggc gtaggcgagt acgtcggggt gttgcatcag
    10321 ccagtttcgg gtcatatttt tcagaacggg tttgtagcct ttggaaccta atccgctgcc
    10381 gtggatgatt tcgccgcata cgccgcgttt ttgggtgaat gcgatgaatt cgttgaggac
    10441 ttttgggctt cttcctgtgt gtagccgtgc aggtcgacat cggtaacgac gggatagtat
    10501 ccgtttttca ggcgttggat gtcgtttttt ccctgtccgt ttttgctgaa gctggcgggc
    10561 gggtcgttgt atgtgctgcc gatgtagaag tagttttctt cgtcggcgcg gttgtctttg
    10621 ggacggactt tgatgggggt tttgtcgggc ggcgcataat attgctgccg gttttttaat
    10681 ggggagactt gtccgactgc ttgtgaaaaa tcgaaatcct gttcttgttt ttgcttgttt
    10741 ttttcggctg cctgtttttc tgccgcttct ttttgggctt gtttgcccag ttgtttgagg
    10801 atgttttgga agtcggtatt catatttttt cctgttattt gtccgatggc tgtttcgggc
    10861 ggggttttaa tttgccggaa tgtttgccaa tcgggggagg atgattttgt tgcctgcgta
    10921 tgttttttga aagtgtgatt gtatatcaaa aagaaatgcg gcaaccgtcg gcagtgttga
    10981 ttgccggaaa tgcggaccgg tcgaaccgat atgcccgaac gcctgataaa gttttaaaaa
    11041 cctgccttgc gaagcaggct gacgtgtttt gccaatcttg aattgccgga aacgcgaaac
    11101 acggaaatct ga
//
LOCUS       DAWGJV010000061        11046 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWGJV010000061 DAWGJV010000000
VERSION     DAWGJV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.061"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="WHQ94_001156"
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="WHQ94_001156"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ2128436.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1611..2486)
                     /locus_tag="WHQ94_001157"
     CDS             complement(1611..2486)
                     /locus_tag="WHQ94_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2128437.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2488..3210)
                     /locus_tag="WHQ94_001158"
     CDS             complement(2488..3210)
                     /locus_tag="WHQ94_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ2128438.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WHQ94_001159"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WHQ94_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ2128439.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3787..4149
                     /locus_tag="WHQ94_001160"
     CDS             3787..4149
                     /locus_tag="WHQ94_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ2128440.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4184..5047
                     /locus_tag="WHQ94_001161"
     CDS             4184..5047
                     /locus_tag="WHQ94_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ2128441.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5145..6104
                     /gene="ttcA"
                     /locus_tag="WHQ94_001162"
     CDS             5145..6104
                     /gene="ttcA"
                     /locus_tag="WHQ94_001162"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ2128442.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6198..9137)
                     /locus_tag="WHQ94_001163"
     CDS             complement(6198..9137)
                     /locus_tag="WHQ94_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ2128443.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPVISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAENFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNIFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTQSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9150..10952)
                     /locus_tag="WHQ94_001164"
     CDS             complement(9150..10952)
                     /locus_tag="WHQ94_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ2128444.1"
                     /translation="MFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARL
                     LRNLPPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYTNSVKMIYIDPPY
                     NTGSDGFVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYI
                     ARELLKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVANVIWQKKYSPQNDAKWFSNM
                     HDYILCVAKNKDIWRPNLLERTEEQNARYSNPDNDERGNWKATDFSVKTYSENYDYPI
                     TTPSGRIVNPPPSRSWRTSKENFEKLLADNRVWFGINGNNVPSIKKFLTEVKQGITPS
                     TILFRENVGDNQEAAKNIRDLFDIPPFETPKPVRLLNYLIKLSSNSDDIVMDFFAGSG
                     TTAHAVMQLNAEEQNGSRRYICVQLPEKTGEKSEARKAGYPTIFDITKARIEKAAAKI
                     RAEHPDYTGDLGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTTWTLY
                     DGAALTTPVEPVRLGSYTAYLCEKRLYLMNAGFTSADLLAFIRKLDDDAAFNPNRVIV
                     FGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2651 a         2951 c         2612 g         2832 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacactctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc cgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaggcgacgg cacaggcaaa cggtgagttg ggcgcggtca agcccgaaat cgccgccgcc
      361 attgaaaaag cctgcgacga agttctgctg aacaaccgct gcctcgacca attcccgtcc
      421 gacgtgtatc agggcggtgc gggaacttcg gttaatatga acaccaacga agtcatcgcc
      481 aacctcgcat tggaagcctt gggatacgaa aaaggccgct acgacatcgt caatccgatg
      541 gaccatgtca acgccagcca atcgaccaac gatgcctatc ccacgggctt ccgccttgcc
      601 gtgtattaca gcatcggcga attgctcgac aaactgactg tcctgaaaaa cgcctttgcc
      661 gccaaagccg aagagtttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtaccgatga ctacaggaca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacgcg ccaactgctg ttggaagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattgcagg ccggttcttc cattatgcct gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgatacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacc
     1261 atttccctct tgggcaatgc agcaatcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatatgcga gcgttacgtt ttcaactcca tcgggctggt tacttatctg
     1381 aatccgtata tcggccacca caacggcgat ttggtcggca aaatctgcgc ccaaaccggc
     1441 aaaggcgtgc gcgaggttgt cttggaacgc gggctgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ctgaaaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcaggga tgctgcttca gacggcattt tgttatatca atcagccgga ttatatgccg
     1621 aacagtacct tgacttcccc ccaataaaac aggcaggcga tagaacacag tgccaacacg
     1681 attcccgccg tattgattgt gttgattttt tccttaaaga acaatgcacc cgaaagcgta
     1741 cccaaaacaa tcacgccgat attcataccg gcaaaaacca aggtcggatt atccttcatc
     1801 atttggtgcg cggtgatgta ggttacgatg ttcataaagt tcaaaccgcc caagaatatg
     1861 ccgccaacca cactctcaac acgccatctg accgatttgg caaacaggca ggcaaacatc
     1921 agcacacccg ccagcacaaa tgcaaccagc aggttgcccg caaatgccgt tccgcttttg
     1981 gcaagctgtt tgaacaggat atcgataatg ccgtaacctg cccacacgcc cagcagcaat
     2041 gccgcctgcc gccacgcgct tcctgatttt ttgccaccgc tgtgtttcca aagcaggcag
     2101 aacagtgcgg caaatgcgag gcacagcccg attagtctgc cttcgctgag tttttcgcca
     2161 aacagcgtca aggcggcaac aatcggcaaa aacagcgaca aacgctgcgc cgcgtcggat
     2221 ttgacgatac cggcggcttc cacagatttg cccattatca cgaatacgga cggcagcagc
     2281 acgcccaaag cggcaaacag cggccacgtc ggcaaaaatg cgccgatatt gccgatatcc
     2341 ggcttcaata ccagcagggt cagtatgacc gcgaccacat aattgacggc gaccgcctgc
     2401 gcgatgtcga tttttttctt ccttgccatt ttcagcaaaa tggaaaccga cacgctgaac
     2461 acgatgctga ttaacaggta agccatacta tatccatttc tcgggtgcaa ccgccctgcc
     2521 gccgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgt tcgttgcaat
     2581 aataatcagc acgccgatac ccaaaagggc agtcagcgca cccaaaatat aaagccaaaa
     2641 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
     2701 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatatagg cgaaaatcac
     2761 gccgactatg ggcatgaaca gcatgattgc acccaaaccg aacaggatgt aggcggcgag
     2821 gacatagttg cgcccgcttc cgccgccgga cggttcgtaa gtatggcacg aacgtgatgc
     2881 ctgccgttca aacgcctgtc tgaacgcctg ttcgcggcgc atctgttcgc ggtaaaactg
     2941 ctccctgcgc gcctgttcgc ggcggaatgc ctcttcctgc cgcccgcgtt cctcatgccg
     3001 tctgaaggac gcgtcgtatt gcatccgttt cgacaggtcg gaaagcgtat cgtaagcccg
     3061 ttggatttct ttgaactttt cttccgcctt cggattgccc ggattgcggt cgggatgata
     3121 tttcatcgcc agcttgcggt aggcgcgttt gatttcgtcc gctcccgcct gcggcgacac
     3181 gcccaatacg gcatacaaat ctttgtccat attgttcctt tatactttcc cgtcaaaccg
     3241 ccgcctgtat tgttttcggg ttcaaaatat ttaaatctcc gggacggatg ccgataatga
     3301 acccgcgttt tccgccgttg atatagatgg tttccaaatc catcaccgac tgttcgacgt
     3361 aaatatccaa ctttgtccgg atgccgaacg gcgttgtgcc gccgaccaga tagcccgtcc
     3421 acttgtttgc ctgtacgggc gtggcgggtt cgatgtgttt cgcacccaaa tgccgcgcca
     3481 gattgcgggt tgaaatctgc ttgtcgccgt gcatcaagac aatcagcccc ttaccgtttt
     3541 catcttgcaa aacaatggtt ttaatgacca agtgttcgtc tttgccgaat agtcgggcaa
     3601 actgcgccgt gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt
     3661 tttcacgcaa aacgcgcacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc
     3721 atgcaaaaca tggaaatcgg tcaagcgcaa tgttaccata tatccccttt atcgaaggaa
     3781 accgatatgg atatccgata tttcggcaca acgccccgct attccgaagc cgtcggcgca
     3841 aacggcttga ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag
     3901 actgccgacg tgcttgccca aatcgaccgc tggctggcgg aatgcggctc ggacaaagcg
     3961 cacgttttgg atgcggtcat ctatctgcgc gatatgggcg actatgcgga aatgaacggc
     4021 gtgtgggacg cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagcgcgg
     4081 ctcgcccgtc cggagtggcg cgtcgaaatc aaaataaccg ccgtgaagaa agatgcggca
     4141 accgcataaa cagatttcct ttatatttca gagtacgcaa atgatgaaaa ccttactgcc
     4201 ctttttcggc gtggtacgcg tcagcggcga agaccgccaa accttcctgc acggacagct
     4261 ttccaacgac atcaataatc ttcagaccgg gcaggcgtgt tatgcgactt acaacacgcc
     4321 caaaggccgt gtcatcgcca atatgattgt cgtcaaccga ggcggcgatt tgctgctgat
     4381 tatggcgcaa gacttgcttg aagcaacggt caaacgtttg cggatgtttg tgttgcgtgc
     4441 caaagccgtt tttgaaatcc ttgaagatta cgccgtcggc gcggaattgg aagcatccgc
     4501 cgaaccgctt gccgcgcaag aacccaacct tgcctttgcc gcacaacaag attcagacgg
     4561 catctgctct atcgctctgc ctcatggcgg catccttcgc atcgccccca aaaacacctt
     4621 gccgccttat gatgccgccg ccgaaaacgc gtggcggctg cacgaaatcc gtagcggtta
     4681 tccgtggata tgcgcggcta ccaaagaaac tgccgttgcc caaatgctca accagcacat
     4741 catcggcggc gtacacttca aaaaaggctg ctatcccggt caggaaatca ttgcccgtgc
     4801 ccagtatcgc ggtcaggtca aacgcggctt ggcggtattg tcgggaaatt cggccgtcga
     4861 agcaggaacc ctgctgacgg cagacggcga agagaccggc atcgtactcg acagcgttca
     4921 agattcggaa aactttaccg cccttgccgt gattaaattc tccgccgcgc aaaaagaact
     4981 gaccgcccca aacggcagta tcttcaaagc ggttcatccc ttctttaaaa ccgaaaatgc
     5041 agaataatcc ctctaatgcc gtctgaacct ttttcagacg gcattttaaa accgcccctg
     5101 ttttcggtta caatgccgca tccccaattt ttcaaaccac cgccatgtcc aaaaaaacca
     5161 aacaagaact ggaaaacaac aaactcagca aacgcctgcg ccacgccgtc ggcgacgcga
     5221 ttaacgattt caacatgatc gagccgggcg acaaaatcat ggtctgcctc tccggcggaa
     5281 aagacagcta tgccctcttg gacatcctgc gccagctcca agccagcgcg ccgattgatt
     5341 tccaactggt tgccgtcaat ctcgaccaaa agcagccggg cttccccgaa gaagtattac
     5401 cgacctatct cgaaagcatc ggcgttccct acaaaattgt tgaagaagac acctactcca
     5461 ccgtcaaacg cgtattagac gaaggcaaaa cgacttgttc gctgtgcagc cgcctgcgcc
     5521 gcggcatcct ctaccgcact gcaaaagaat tgggctgcac caaaatcgcc ttgggacacc
     5581 accgcgacga catcctcgcc accttgtttt taaatatgtt ttacggcggc aaactcaaag
     5641 ccatgccgcc caagctggtg agcgacaacg gcgaacacat cgtcatccgt ccgctggcgt
     5701 atgtaaaaga aaaagactta atcaaatacg ccgaattaaa acaattcccc atcatcccgt
     5761 gcaacctctg cggctcgcaa cccaacctgc aacgccaagt catcggcgat atgctgcgcg
     5821 attgggacaa acgctttccc ggccgcatcg aatcgatgtt ctccgccctg caaaacgtcg
     5881 tcccttccca ccttgccgat accgaactct tcgactttgc cggattggaa cgcggtcaaa
     5941 ccctgaaaca cggcggcgat ttggcgtttg acagcgaaaa aatgcccgaa cgtttttcag
     6001 acggcagcga agaagacgaa agcgaaatca aaatcgagcc gcaaaaagcc gaacgcaaag
     6061 tcatcaacat tctggcgaac aagccgaaaa cctgcggcgc ataaacctcc gccgtcaaaa
     6121 atgccgtctg aagcccgttt ggcgtttcag acggcatttt aaaatcccga agcagacggc
     6181 ttccaaacat ttgtttctca gtgtccgttt tcagaatgcc cgcctgcggg catattttgc
     6241 ccgatcagtt cggctatcct ctcgccgtca aactgcgttt tgaacaccac cttgatttct
     6301 ttggaaatct cggcaaacag cttttcggca tgtttgattt tccgctcttc gctttttcgc
     6361 aaatcttccg ccccgtcagt ccctttggct tcaatcacaa agttcagaat ctcgccgctt
     6421 tgggttttca cgatataggc aaaatcgggc gaatacgtgc cgccgccggc aacggggatt
     6481 ttgatggagt ttctcggtat tttagtgaat acggttacgc cttcaatttg gttgttggcg
     6541 acattttcat gttctatatc cgaatcgtag aaaatctcgt cgaagagata gccggcggca
     6601 ggccggtgct ccgtatcttc aaatcttccc aaatctgctt ttttcaccgc gcgcggtttg
     6661 ccgtctttat cggtaaattt ggtcggatgg attttgctgc cgacaagccg gtaatccagt
     6721 tcgaatttat ggaaggaatg atgaagcaaa aaccggttga agccgttttt gatttgggcg
     6781 atggtctgca tattcaaaaa atcgccaatg ttcagttcgt cgcggacgcg gtaaaacgcc
     6841 tgatgcaaag tctgcatgcg gatttttgcc gtttgtgcca gtttttccag aaactctcgg
     6901 taagtcattg tgttgaaacg gataaaatct tcatcttcaa tactgtctat gcggcgggaa
     6961 agcataagcc cgttgttgat ataagcttcg tttaccgccg tgcgtatgcc tgcctgcggg
     7021 aatttggcgg cgttttcacg caaataggcg gtaaataaat cgacaaattc ggcttcatcc
     7081 ttgattttgt actgcaaaac ggctttatgg tgaatcagct cccacaaggc cttgagttct
     7141 tcatatttgc cttcgcgcat gatgatggtg tctttgccct cgtctttggc gttgctgact
     7201 ttgcctttgt ccaaaccttt ggggaaggct tcgggatagg cggcttttaa tttgtcatag
     7261 ccgtctttgg taaagttttc attgtcgtca atgatgccgt ctgaaaacag ttggtttacc
     7321 aataccagcg gtttgatatc ggggtatttt tgcaatattt tttgtttcag ctcttcggta
     7381 aactttttgg agatttcttc ctgaaaagaa ttatcgttga tttcgccgac aagctgcttc
     7441 acaaagtctt tttcgctgct atcgacaaaa taattcagtt tgtacggcac atcgcgcacc
     7501 cgcgccatca gctcgtttac cggcaggcgc aggccgcgtc cgacttcttg cagcttggaa
     7561 gtcgtgctgc cgctggaacg cagtttgcaa atctggaaaa tgttgggatt gtcccagcct
     7621 tcacgcagcg tccatttgga aaaaataaag cggcgcgggt tgtccaaaga cagcagtttt
     7681 tccttatcgt gcaggatttc attgatttcc tgctcgattc tatcgtcgct gtctgtattg
     7741 tctttggaaa aatagccgcc gtggcaggcg gatacatcgt ccaacgtctt ttgcaggtaa
     7801 tcgcggtaaa acgggtcgct ttccgttttc agacggcgtg ccgcttccgc gcgaatccag
     7861 ctttcaaatt tatctttcag gctgcctgaa agctcgttgc cgctgcggta gcccgcgata
     7921 tcgtcaataa aaaacagcgt cagcggcttg attttgggct gtggcgcgcg ttctgccaaa
     7981 agcgcacgtt ccagcttgaa attctcggca accgccctct gcatcatcgc atcctgcacc
     8041 gtttgcgaat aggaataagg gttgatgacg gcacccgttt tcaactccaa gccgttgctt
     8101 aacaccacca cggttttatt cattttgtcg attttcaaat ccgaaataac cggatggatt
     8161 tgcgccaaat cttcgccttt tgccagtttg aacgtctgct ttttgtcctt ttcgtttaat
     8221 tcaaatttcg cttctttgcc gtccgacgac accagtttta ccgccgcatc catgccgccc
     8281 tgcatttctt cctgaaacac gcgcacgcct ttgaccagcc cgtcgttaaa cgcgtctact
     8341 gccgtcaaac ggtaaagcaa gttgtaatat tcatcgttaa atgttgcacc gtagcgcaaa
     8401 atatattgcg gttttaagcg tttgatattg ccccacgttt tcgcgctatc tcgggtcggg
     8461 aatttatgcg gttcgtccac aatcataaac gggcgcacgg cggccaatgc atcaacggga
     8521 ttgtcaaaca aatccttcaa tgccttgtcg cccgtatcgt tcatggacga cgaattaacc
     8581 atgcccgcgt taatcagcag cacatgaatt tcctttttgt tttccgcttt gacaaattgc
     8641 tcaatcgtta tgggcgcatt ggactttttg cccttattct ttttcgcgct ttccaccaca
     8701 taggttttca ggcgtacgcc ttcataatcg ccgccgaaat cctgttcaaa atgctctgcc
     8761 aaagccttgc tttgcaaaaa ctgctgtgtt cccgccttaa tggacaaagt cggcacgacc
     8821 acgataaatt tgaacacgcc cagccaacgg tgcagctcga acatggtttg tgtgtaggta
     8881 taggttttgc ccgtgcccgt ttccatggaa atatcaagga tattttggtc gtccgaacgg
     8941 tcggggaatc ggccgtctat accgttttgg ctttgcactt tcaggatatt gtttgcgtat
     9001 tgttttgcag caaacaaaag ttcgggattt tcgtctgccg tccgatattt gggcgttgcc
     9061 ccgtcaaaca cgcccaaaac cgccgaaacc gcccgcattt ggtgcggctg gtttttctcg
     9121 taattaaaac cgctcatgaa ttgcctccgt caaaccctga taaccacatt caactcaatc
     9181 tcttttttat tggcataacc gcgaaccgcc tggtcaagtt cgtgctgcat ggcgcttgcc
     9241 atattgctgc cgaatacaat cacgcggttg ggattgaaag ccgcatcgtc gtccagcttg
     9301 cggataaacg ccaacaaatc ggcagaagta aaaccggcat tcatcagata cagccgtttt
     9361 tcgcacagat acgccgtata actgcccaac cgcacaggct caaccggcgt ggtcagtgcc
     9421 gccccgtcat acagcgtcca ggtggtcaga agcgtttgca gctgttcttc gcttaattca
     9481 tcgttaagcg gcaaatccgg ttgttcgggc gaaaaatcct tgtccggatg ctgtctgaaa
     9541 ttgtctgccg tttgaaagat tttgaagccc aaatcgcccg tgtaatcggg atgttcggcg
     9601 cggattttgg cggcggcttt ttctatgcgg gctttggtga tgtcgaagat ggtcgggtag
     9661 cctgctttac gggcttcgga tttttcaccg gttttttcag gcagttgcac gcagatatag
     9721 cggcggctgc cgttttgttc ttcggcgtta agctgcatca cggcgtgtgc ggtcgtgccg
     9781 ctgcctgcga agaagtccat cacgatatcg tccgaatttg aactaagttt aataagataa
     9841 tttaataacc taacaggttt tggagtttca aaaggtggaa tgtcaaataa gtctctaata
     9901 ttttttgcag cttcctgatt atcaccaaca ttttctctaa ataatattgt tgaaggtgtt
     9961 attccttgtt ttacttctgt taagaatttt ttaattgagg gaacattatt accatttatt
    10021 ccaaaccaaa ctctgttatc tgctaataat ttttcaaaat tttcttttga agttctccaa
    10081 cttctactag gtggcggatt aacaattcgt cctgatggcg tggtaattgg atagtcataa
    10141 ttttctgaat atgttttcac agaaaaatca gttgctttcc agtttccacg ttcatcatta
    10201 tcaggattgg aatatcttgc attttgttct tctgttctct ctaataagtt tggtcgccaa
    10261 atatctttat tttttgcaac gcataaaatg tagtcgtgca tatttgaaaa ccattttgca
    10321 tcgttttgtg ggctatattt cttttgccaa atcacattag caacaaaatt cccttcccca
    10381 aacacttcat cgcaaagcaa cttcaattgc gccgcttcgt tatcgtcaat cgagataaaa
    10441 atcacgccgt cgtcctttaa cagttcgcgg gcgatataca ggcgcggata cataaaggtc
    10501 agccacgcgc tgtgcgagtt tgagcctttg tcggtgaaat ctaaaatccg cgcggcttcg
    10561 tcttcgtcaa tattcgccag gagggcaagt tcggcgggtg tgaatttgcg gtcgtcctga
    10621 tagacaaagc cgtccgagcc agtgttgtag ggcgggtcga tgtaaatcat cttcacgctg
    10681 tttgtatagg cgttttttaa gtgtttcaac acttctagat tgtcgccacg aatcagcagg
    10741 ttttggctgc ctgcattttc gggcttggcg ttgtgcgtct tatcttcact tatcagggtt
    10801 tcgggcggca ggttgcgaag caggcgggca tatgatttgc ccaaccagtt catttcgtaa
    10861 aattcgcgtc cgatgtcggt ctgtggcgca atttcggctt gtaatctgtc gataaggaaa
    10921 tttccgtctg cgtcaaaaca ggcgggaaac agttttttga gctgttcgag ttgggtagag
    10981 ttggcggtaa tgccgtctga agtgtagatt gcctcggtgt tcgccccggc tgtgtcgctt
    11041 agatca
//
LOCUS       DAWGJV010000062        10570 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWGJV010000062 DAWGJV010000000
VERSION     DAWGJV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.062"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(10..1101)
                     /gene="mltB"
                     /locus_tag="WHQ94_001165"
     CDS             complement(10..1101)
                     /gene="mltB"
                     /locus_tag="WHQ94_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ2128445.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDTAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRAVVDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1250..2677)
                     /locus_tag="WHQ94_001166"
     CDS             complement(1250..2677)
                     /locus_tag="WHQ94_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ2128446.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2862..6752
                     /gene="mfd"
                     /locus_tag="WHQ94_001167"
     CDS             2862..6752
                     /gene="mfd"
                     /locus_tag="WHQ94_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ2128447.1"
                     /translation="MTYPIPKPREKSRWLNLSQGSLPLALARYLPHRRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDNEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPSQQQAAPASEGEGDAITDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVANNLYGQTRQQAAPSPVGEDW
                     GESKTVAAQTEFSVSATNPLPSPLPQEREQSTAAISDSLKTKSSLYPIENSLHGQIQQ
                     QPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDL
                     AEINIGDPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRY
                     SGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDY
                     QAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMG
                     GKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDI
                     VIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLS
                     MALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTI
                     ENMRERLETLLPEARISVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNA
                     NTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELG
                     AGFTLAMQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPL
                     GITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQP
                     VKTLIESHHLRLMAKELGINAIDAAGEAVTVTFGKNNNIDPTEIILLIQNDKKYRLAG
                     ADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6901..7284
                     /locus_tag="WHQ94_001168"
     CDS             6901..7284
                     /locus_tag="WHQ94_001168"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ2128448.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7306..8148
                     /gene="kdsA"
                     /locus_tag="WHQ94_001169"
     CDS             7306..8148
                     /gene="kdsA"
                     /locus_tag="WHQ94_001169"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ2128449.1"
                     /translation="MDIKINDFTLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8163..8603
                     /locus_tag="WHQ94_001170"
     CDS             8163..8603
                     /locus_tag="WHQ94_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ2128450.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSALGGSFGMWHGISSIVFMAAALLSIYLSWRLYGKNAV"
     gene            8648..9934
                     /gene="eno"
                     /locus_tag="WHQ94_001171"
     CDS             8648..9934
                     /gene="eno"
                     /locus_tag="WHQ94_001171"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ2128451.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYLGKGVLKAVDHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            9948..10226
                     /gene="ftsB"
                     /locus_tag="WHQ94_001172"
     CDS             9948..10226
                     /gene="ftsB"
                     /locus_tag="WHQ94_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ2128452.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10267..10557)
                     /locus_tag="WHQ94_001173"
     CDS             complement(10267..10557)
                     /locus_tag="WHQ94_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ2128453.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2533 a         3090 c         2703 g         2244 t
ORIGIN      
        1 taaaaaacat tacaatcccg ggccgccaag cgaattggca atgtccctga ccgccgttac
       61 atacatccgg ctgtggttgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
      121 ttcaaacacg cccggtgcgg tttccagttt gaacaaaacc gccttttcat catctgcaag
      181 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
      241 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagcat
      301 tttaccgccc gtgcgccaac cgtgctgctt catataattg gcaaccgatg ccgcgacatc
      361 gccgacgttg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
      421 gtagctcgaa ggcataaatt gcggcatacc cattgcaccc gcatagctgc ccttaaaggc
      481 gaaaacatcg ccgccctctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
      541 aaacccggcg cggcgggggt aatcgaaacc taaagtcgcc aatgcgtccg ccacgcggaa
      601 actgcccgta tttttgccgt aattcgtttc aatcccgata atcgccacga taagctcggc
      661 gggcacgccg tatttttgcg ccacatcatc gacaactgcg cggttttccg cataaaaccg
      721 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
      781 cgatgtggag ggtcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
      841 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
      901 gcggacattt gcattggcgg caaacccgct gtcggatacc ggtacggctg ccgtgtcaaa
      961 cgcggggcgg ctttcttttt tcatttcatc cgcgcggggg gcttgggctt catttacccg
     1021 gggcgggcgt gcctccatcg ccgtacaggc agacaaagcc gccaaacaaa ttgccagcgg
     1081 cagtattttt ctctttttca taaatatgtt ccgaacaaat agggtaagtg ggaaagcggc
     1141 gcaaggggcg cggtgcgaaa tgcggcatcc gccgcaatcg gcggctttgt ggaataccgc
     1201 gcgttgcctt ttgcaccgcc tgaaatccgt atgccgccgt atttgcccgc tatccgcaat
     1261 atcggcagtc caatatatct ttgcggatgt cgttcagcag gaaggctgcg gtgtcttcgt
     1321 gttccgcctg tacgaagaca aagagtcggg cgatgatttt gccgatgaag actttttgca
     1381 gggcgcaggc gtcggtcagg gtgtgttcct gcaggaagct gcggatgtct tcgggcaaag
     1441 tgtagtcgcg ggcgacggcg gcaaagcggg catcggtttg caggcggtcg agcagggttt
     1501 ggtttttgtc caacttcatg cctgcttctt tttcttcggc ggtggcgcgg acgaactggt
     1561 cgtaaatttc ggcataaagc atatcgttcg ggccttcaaa aatcgtgaag gggcggatgt
     1621 cgatagcgat attgccggcg gtgtgtccgc gttcaaaacc cttcgcgccc aaaagttttt
     1681 gcaacatttg tgcggcggcg taagtgtatt ccgtggcgag ggttttgacg atgttcgcct
     1741 ccatcagctg atgggcgacg ggggcaacag gtgaaacgga atggcagacg tagcggtaaa
     1801 gaatctcgga aacctgatgg cggcgccgga tttcgcggcg ttcgtaatcg acgaatctga
     1861 tgtcgttgcg gacgtatcgt tccaggtttt caaggatgta ttccataatg ccgtgcgtca
     1921 tgccgatcag ttgcaggcgg ctgcggataa agatgttttg gaacgcgcgc aaaccggcag
     1981 cgtcgctctg ggagagtttc atcacggcgg ttgcaggcat ttcggcatcg atgcggttga
     2041 cggcgtaacg gacggcgcgc aagccttcgg atgcgagggt ttcgcagcgg atgtatgttt
     2101 tggggacgag cagcaggtcg atgactttgg cgagtttgcc gtttttgcgc tctttggcgg
     2161 caacgaggag gaagtcgctt tgcgagttgc cctgccagta tttcgcggcg ttgacgtaaa
     2221 tggtttgtcc gtcggtatat tcgtagtaag actgcatttc tcgggcaatc gccgcgccgg
     2281 aggtttcggg ttcggtaacg cccaaaccac cgccctcgcc tttgaaaacc atgtccaaac
     2341 cctgtgcgat ttgcgcttca tcgccgaact cttgcagtgg ctgcaacacc agcgcgcctt
     2401 cgatgccggt acgcagcgta acgggcacgc cgtaatgccc cgcaatccgc aagacttcct
     2461 gaatttcaaa ctggctgccc ttgcgcccgc cgtgtttttt gtcgaggaag ggcaacagca
     2521 aacccgcctg cttcaaggca agccatttgt cttcgggcag gtatcgcatc aggtcgatac
     2581 cgtctgaaaa aatgcggcgg aatgcggatt cgatgtgctt taaaaaagca gccgtgtcca
     2641 tagttgacgg ctgcgcactc ggttcggtgt gtatcatcgg cttcctctgt cggttcccat
     2701 taatcggcgg ccggtcaaac cgcctgccac ggtttagagt taattttcta aactttacca
     2761 caaagtgcgc cgggcaacaa tccgccgacc tttcagacgg catcgcgccc cctcccgtgc
     2821 taaaatgacc gtttgcatca ctgtccgccg attgccgcac tatgacctac cccatcccca
     2881 aaccccgtga aaaatcccgt tggctcaatc tgtctcaagg ctcgctaccc ttggctttgg
     2941 cgcgttatct gccgcatagg cggctcaaag tcgtgctgac ccaagatgcg gaacaggcgc
     3001 tacgccttca gacggcgtgg cggtttttcc gtccgcacga tacggcggtg ttcctgccgg
     3061 actgggaaac gctgccttac gagcgttttt cgccgcatca ggatttggtg tcggagcgtt
     3121 tgtcggcgtt gtggcagatt aaaagcggcg cggcggacgt gttgttcgtg ccggttgcca
     3181 cggcgatgca gaagctgccg cccgtgccgt ttctggcggg gcgcacgttt tggctgaaaa
     3241 cgggacagac tttggatata ggccgtctga aaagcgattt ggtggatgca ggttataacc
     3301 atgtttccca cgttgtcgcg gcaggcgagt ttgccgtgcg cggcggcatt gtggatttgt
     3361 tcccgatggg cagcgaaatg ccgtaccgca tcgatttgtt cgacgatgaa atcgacagca
     3421 tcaaaacctt tgacaccgag acgcagcgca ccatctcccc cgtttccgaa atccgtctgc
     3481 tgccggcgca cgagttcccc accgacaacg aggcgcaaaa aatcttccgc agccgttttc
     3541 gcgaggaagt cgatggcaat ccgaacgatg ccgctgtgta caaagccgtc agcaacggtc
     3601 atttcggcgc gggcgtggaa tactacctgc cgctgttttt tgaaaacgag ttggaaacgc
     3661 tgtttgacta tatcggcgaa gatgcgctgt ttgtctcttt aggcgatgtt catgccgagg
     3721 caaaccgttt ttggagcgat gtcaaatcgc gttacgcgat ggcgcagggc gacgaaacct
     3781 atccgccttt gcttccacag tatttgtatc tctctgccga tgtgttcgca ggccgtctga
     3841 aaaactacgg acaggtgctg cccgatgttt ccggcaagga atacaccctg cccgaccttg
     3901 ccgtcaaccg ccaagcagat gagccgttgc aggcattgaa ggattttcag acggcgtttg
     3961 acggacggat tttgctgtgc gccgaaagtt tgggacggcg cgaaactatg ctcggtttct
     4021 tgcagcaaaa cggtttgaaa gccaaacccg tgtccgactg gcagggcttt ttatcggcac
     4081 acgagccgct gatgattact gttgcgccgt tggcatacgg gttcaaactg ggcgggctgc
     4141 aatctccgag ccaacagcaa gctgctcccg cctccgaagg agaaggcgat gcgattaccg
     4201 accaaaccga attttccgca tccgcaacaa accctctccc tagccctctc ccgcaggaga
     4261 gggaacaaag tgccgccgcc gtttcagacg acctgaaaac caaaagcagc ctgcatcccg
     4321 tcgcaaataa tctgtacgga caaacccgac agcaagctgc cccctccccc gtgggggaag
     4381 attggggaga gagcaaaaca gttgccgctc aaaccgaatt ttccgtatcc gcaacaaacc
     4441 ctctccctag ccctctccca caggagaggg aacaaagtac cgccgccatt tcagacagtc
     4501 tgaaaaccaa aagcagcctg taccccatcg aaaatagtct gcacgggcaa atccaacagc
     4561 aacctgcccc ctcccccgtg ggggagggtt ggggagaggg caaagcagtt gccgctcaaa
     4621 gtgccatcgc cgtcatcacc gaatccgatc tctaccaata cgtcgcccgt tcgcgcatcc
     4681 acaaccgccg caagaaacac gccgccgttt cagacgacct gttgcgcgac cttgccgaaa
     4741 tcaacatcgg cgaccccgtc gtgcacgagg aacacggcat cggccgatat atgggcttga
     4801 taacaatgaa cttgggcggc gaaaccaacg aaatgatgtt gctcgaatat gcgggcgaag
     4861 cgcagcttta tgtgcctgtt tcgcaactgc atttaatcag ccgctactcc ggtcaggcgc
     4921 atgaaaacat tgccctgcac aagctcggca gtggcgcgtg gaacaaggcg aagcgcaaag
     4981 ccgccgaaaa ggcgcgcgat accgccgccg agttgcttaa cctttacgcc cgacgtgccg
     5041 cccaatcggg acacaagttt gaaatcaacg agttggatta tcaggcgttt gccgacggct
     5101 tcggctacga agaaaccgaa gaccaggccg ccgccatcgc cgccgtgatt aaagatttga
     5161 cgcaggcgaa gccgatggac cgcctcgtgt gcggcgatgt cggtttcggc aaaaccgaag
     5221 tcgccctgcg cgccgcattt gtggcggtga tgggtggcaa acaggtcgcc gtactcgccc
     5281 cgaccacgct tctggtcgag cagcacgcgc aaaactttgc cgaccgtttc gccgatttcc
     5341 ctgtgaaagt cgccagcctt tcgcgtttca acaacagcaa agccactaaa gccgcgctgg
     5401 aaggcatggc ggacggcacg gtcgacatcg ttatcggcac gcacaaactg gtgcaggacg
     5461 acatcaaatt caaaaactta ggtttagtga ttatcgacga agaacaccgc ttcggtgtcc
     5521 gccaaaaaga gcagctcaaa cgcctgcgcg ccaatgtgga catcctcacc atgaccgcca
     5581 cgccgattcc gcgtacttta agtatggcgt tggaaggact gcgcgacttc tcgctgatta
     5641 ccaccgcgcc cagccgccgc ctcgccgtca aaacctttgt caaacccttt agcgaaggca
     5701 gcgtgcgcga agccgtgttg cgcgaactca aacgcggagg acaggtattt ttcctgcaca
     5761 atgaagtaga tacgattgaa aatatgcgcg agcggctgga aaccctgctg cccgaagccc
     5821 gcatcagcgt ggcgcacgga caactgcgcg agcgcgagct ggaacaagtc atgcgcgact
     5881 ttttgcagca acgatttaac gtgttgctct gctccaccat catcgaaacc ggcatcgaca
     5941 tccccaacgc caacaccatc atcatcaacc gcgccgacaa attcgggctg gcgcaactgc
     6001 accagcttcg cgggcgcgtc ggccgcagcc accatcaagc ctacgcctac ctgctcacgc
     6061 ccgaatacat caccaaagac gcagaaaaac gcctcgacgc catcgcggcg gcagacgaac
     6121 tcggcgcagg tttcaccctc gccatgcagg atttggaaat ccgcggcgca ggcgaaatcc
     6181 tcggcgaagg gcagtccggt gaaatgatgc aggtcggctt cacgctctac accgaaatgc
     6241 tcaaacaggc cgtgcgcgac ctcaaaaaag gccgccaacc cgacctcgat gcaccgttgg
     6301 gcattaccac cgaaatcaaa ctgcacagcc ccgccctgct gcccgaaagc tactgccccg
     6361 acatccacga acggctcgtc ctctacaaac gcctcgccgt ctgcgaaacc gtacagcaaa
     6421 tcaatgccat acacgaagaa ctcgtcgacc gcttcggact gcccgagcaa cccgtcaaaa
     6481 cccttatcga aagccaccac ttacggctta tggcaaaaga attgggtatc aatgccattg
     6541 atgcggccgg cgaagcggta acggtaacct ttggcaaaaa caacaatatt gatccaaccg
     6601 aaatcatcct gctgattcag aacgacaaaa aataccgcct tgccggcgcc gataagctgc
     6661 ggtttaccgc agagatggaa aatatcgagg tcagaatcaa caccgtaaaa aacgttttaa
     6721 aaaccttgca aaacagatgc ctgcccaaat aaagccgaca ccgcaatgct gtctgaaaca
     6781 ccgttttcct tgtccgaaag ccgccattat gaatttgaag gaaactccac tataatacgg
     6841 cattcagatt ttcagacggc atcgcgcccg tcaaaccgca cacaaaccaa aaggaaatac
     6901 atgttccgta ctatgcttgg cggaaaaatc caccgcgcca ccgttaccga agccgattta
     6961 aactatgtcg gcagcattac cgtcgatcaa gacctgttag acgcggcagg catctacccc
     7021 aacgaaaaag tcgccatcgt caacaacaac aacggcgaac gttttgaaac ctataccatt
     7081 gcagggaaac gcggcagcgg cgtgatttgt ctgaacggtg ctgcggcaag gctggtgcag
     7141 aaaggcgata tcgtcatcat catgtcttac gtccaactct ccgaacccga aatcgccgcg
     7201 catgaaccca aagtcgtctt ggtagacgga aacaacaaaa tccgcgacat catctcctat
     7261 gagccgccgc acaccgtgct gtaattccgc aaacggacat cgattatgga tattaaaatc
     7321 aacgacttca ccctcggcaa caactcgccc ttcgtcctat tcggcggcat caacgttttg
     7381 gaaagcttgg attccaccct ccaaacctgt gcgcattacg tcgaagttac ccgaaaactc
     7441 ggtattccct atatctttaa agcctctttc gacaaggcaa accgttcctc catccattct
     7501 tatcgcggcg taggcttgga agaaggctta aagatttttg aaaaagtcaa agcagagttc
     7561 ggcatccccg tcattaccga cgtacacgaa ccccatcagt gccaacccgt cgccgaagtg
     7621 tgcgatgtca tccagcttcc cgcctttctt gcgcggcaga ccgatttggt ggccgcaatg
     7681 gcggaaacgg gcaatgtcat caacatcaaa aaacctcagt tcctcagccc ctctcaaatg
     7741 aaaaacattg tggaaaaatt ccacgaagcc ggcaacggga aactgatttt atgcgaacgc
     7801 ggcagcagct tcggctacga caacctcgtt gtcgatatgc tcggtttcgg cgtgatgaaa
     7861 cagacttgcg gcaacctgcc ggttattttc gacgttaccc attccctgca aacccgcgat
     7921 gctggttctg ccgcatccgg cggtcgtcgc gcacaagctt tggatttggc acttgcaggc
     7981 atggcaaccc gccttgccgg cctgttcctc gaatcgcacc ccgatccgaa actggcaaaa
     8041 tgcgacggcc ccagcgcgct gccgctgcac cttttagaag attttttaat ccgcatcaaa
     8101 gcattggacg atttaatcaa atcacaaccg attttaacaa tcgagtaaca cggtttcgcc
     8161 ttatgatgca gactttccga aaaatcagcc tgtatgccgc aaccttatgg ctcggtatgc
     8221 agattatggc gggttatatc gtcgcgccgg tgctgttcaa aatgctgccc aaaatgcagg
     8281 cgggcgaaat tgccggcgta ttgttcgaca tcctctcttg gagcgggctt gccgtttggg
     8341 gcgcggtact ggctgccgcc tttgccgccc taacccggcg gcaaaccgcc ctgctgcttt
     8401 ttttattgtc cgcccttgcc gccaaccgat tcttgattac acccgttatc gaggcgctga
     8461 aatacgggca cgagaattgg ctgttgtcgg ctttgggcgg atctttcgga atgtggcacg
     8521 gcatttccag tattgttttt atggcggccg ccctactttc gatttactta agttggcggc
     8581 tctacggcaa aaatgccgtc tgaagcctac cgttttacct cacgtcactt taatttggag
     8641 aatatttatg agcgcaatcg ttgatatttt tgcccgcgaa attttggact cacgcggcaa
     8701 cccaaccgta gaatgcgacg tactgttgga atccggcgtc atgggacgcg cagccgtacc
     8761 aagcggcgca tcaaccggtc agaaagaagc tttagaactt cgcgacggcg acaaatcccg
     8821 ttacttgggc aaaggcgtat tgaaagccgt tgaccacgtc aacaaccaaa tcgcgcaagc
     8881 cctcatcggt atcgatgcca gcgaacaatc ttatatcgac caaatcatga tcgaattgga
     8941 cggtactgaa aacaaaggta acttgggtgc caacgcgact ttggccgttt ctatggccgt
     9001 agcacgcgca gccgctgaag atgcagggct gccgctttac cgctacttgg gcggcgcagg
     9061 tccgatgtcc ctgcccgtac cgatgatgaa cgtcatcaac ggcggcgaac acgccaacaa
     9121 cagcctgaac atccaagagt ttatgattat gcccgtcggc gcaaaatctt tccgcgaagc
     9181 gttgcgctgc ggtgcggaaa ttttccacgc cttgaaaaaa ctgtgcgaca gcaaaggctt
     9241 cccgaccaca gtcggcgacg aaggcggttt cgcccccaac ctgaacagcc acaaagaagc
     9301 cctgcaactg atggttgaag ccaccgaagc cgccggctac aaagcgggcg aagacgtatt
     9361 attcgcattg gactgcgcgt ccagcgagtt ctacaaagac ggcaaatacc acttggaagc
     9421 cgaaggccgc tcctacacca acgcggaatt tgccgaatat ctggaaggcc tggtcaacga
     9481 gttccccatc atctccatcg aagacgggat ggatgaaaac gactgggaag gctggaaact
     9541 gctgaccgaa aaactgggcg gcaaagtcca actcgttggc gacgacctct tcgttaccaa
     9601 cccgaaaatc cttgccgaag gcattgaaaa aggcgtggca aacgcactat tggtcaaagt
     9661 caaccaaatc ggtactttga gtgaaaccct gaaagccgtc gacttagcca aacgcaaccg
     9721 ctacgccagc gtaatgagcc accgctccgg cgaaaccgaa gacagcacca ttgccgactt
     9781 ggcagtcgcc accaactgta tgcagatcaa aaccggttct ttgagccgtt ccgaccgcat
     9841 ggcgaaatac aaccaactgc tgcgtatcga ggaagaattg gcggaagccg ccgactaccc
     9901 cagcaaagcc gcattctacc aactgggcaa ataaaaaagg ttaaggtatg aagtgggtaa
     9961 ctgtcgtttt atccttcgca cttgtctgtt gccaatacag cctctggttc ggcaaaggca
    10021 gcatcggacg caacagcagt ctgagagaac agattgccgt tcaagaagaa aaaaaccaga
    10081 cactcgccct acgcaatcat tcccttgccg ccgaagtcta tgatttggaa aacggtcaag
    10141 aagccatttc ggaaatcgcc cgggtagaac tgggttatat ccaagacggt gaaacctttt
    10201 accgactcat caggcataac cggtaatacc gtcaaaaagc cgtccgaacc aatgttcgga
    10261 cggcttttat ttcaacaaac tgtcagacag cccctcatcc tcccccgaca aaccgcaatc
    10321 cagcctgaca tccccctcga cgcaacagca gcacggcagt atctcgtccc gccccaaaaa
    10381 agccaaaggc ggctcccgat aagtaacgct tccctccaaa atcttcactc ggcacgatcc
    10441 gcaatatccg cttcggcact gatattccac catatgcccc gtccgttcca agccttccaa
    10501 cagggtctcg ccctccaaga gctcaaaaaa acccttattc gtaccaatgc gcgccatttc
    10561 cgaccaatca
//
LOCUS       DAWGJV010000063        10270 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWGJV010000063 DAWGJV010000000
VERSION     DAWGJV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.063"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(99..1190)
                     /locus_tag="WHQ94_001174"
                     /pseudo
     CDS             complement(99..1190)
                     /locus_tag="WHQ94_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
     gene            complement(1320..1868)
                     /locus_tag="WHQ94_001175"
     CDS             complement(1320..1868)
                     /locus_tag="WHQ94_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ2128454.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(2023..2394)
                     /locus_tag="WHQ94_001176"
     CDS             complement(2023..2394)
                     /locus_tag="WHQ94_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ2128455.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVQAANPQAALAQYFGRGGR"
     gene            complement(2450..2557)
                     /locus_tag="WHQ94_001177"
                     /pseudo
     CDS             complement(2450..2557)
                     /locus_tag="WHQ94_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2689..4380)
                     /gene="dld"
                     /locus_tag="WHQ94_001178"
     CDS             complement(2689..4380)
                     /gene="dld"
                     /locus_tag="WHQ94_001178"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ2128456.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4547..4643)
                     /gene="ffs"
                     /locus_tag="WHQ94_001179"
     ncRNA           complement(4547..4643)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WHQ94_001179"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4904..8737
                     /locus_tag="WHQ94_001180"
     CDS             4904..8737
                     /locus_tag="WHQ94_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ2128457.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAIFD
                     VHTLDSDGLNVVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELADIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKTLRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQIIHFINRPLPKNVPAKTPRSLLGIEDDKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPTDYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            8813..9814
                     /locus_tag="WHQ94_001181"
     CDS             8813..9814
                     /locus_tag="WHQ94_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ2128458.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(9876..10058)
                     /locus_tag="WHQ94_001182"
     CDS             complement(9876..10058)
                     /locus_tag="WHQ94_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128459.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
BASE COUNT         2328 a         2794 c         2803 g         2345 t
ORIGIN      
        1 gggtttctgt tttttccgat ggattcctgt tgcgttgggg gctggattcc cgctttcgcg
       61 ggaatgacgc ggtgggggtt tcggtttttt ccgcctgttt attttgcggc ttcgattgcc
      121 gctatttctt tgcgtaggtg tttgattgcg ggggttacga tggcgacaaa catcgcttcg
      181 cggacgcgcc tttgggcggg actgcgcatc aggtagcctt ttgcgccgga atgcagtgcg
      241 gcggattggg cggcggcaag tgccagtacg gcggcggctt cgcgcagctt gagggtagca
      301 aggttgtcgg gcgtgccgct ccaagccaag ccggcgagcc gttcggtttc tgcccacgcg
      361 ccgtccaacc ttgctttgag gctgtcgtag ccgtcgtcga gatagctgtt gacttctgca
      421 ttgacgatgt tggcgaggcg gatgatgccg aggctgccgt ctatcacgcc cgcgccgatg
      481 ccgatttgca ggaggataaa gcctgctttg atgctttgga tgtagtcggc aaactgttcg
      541 ggcgcggcga tgatgtcttc gtcggggata aatacgtctt tgaagttcag gctgaaggtg
      601 cgcgtgcctt cgagggcgca aaattcgggg cagttttgca ggcttacgcc ttcccattgt
      661 ccgccggtga taaacataac gtaaccgtcg ccgatttggg cggtattcgc ctagatgtgg
      721 tcttcgccga tgttggacac ccacggcagc gcgccgttga ctgtgtagcc gccctccact
      781 cgctcagctt ggaggttgtg tttttcgatg ccggcaaggt gtttgacggt attggacatg
      841 cccgtacccg ccaatacttt gccttgcagg atgtcggcga ggtatttgtc tttgacggct
      901 tggttgggcg tttggtgcag ataccacgcg caagccgcct gacaccacgc gctgaaggag
      961 gttgcgccgc attctttgcc gatttcgcgc aagacggcga tttgcgttgc caaacccaag
     1021 ccgttgccgc cttcggcttc tgtaccgact gcgccgaatc cgccgattgc gccgagttcg
     1081 cgcataaatg cttcggggta gtatcctttg cggtcgatgt cgtccactat gggtttgagc
     1141 ttggttttga cgagttcggc aacgttggca atcagggttt gggcgttcat ctttgttcct
     1201 taaggtttgc gggggaatcg ggcgcacgcc tgatgcgcgg ctgcttccct gccgtttggc
     1261 ggcttgtttt tgtttgaggg gggttgaaaa accgcccgat attcgggcgg tttgccgtat
     1321 caggcgtaag cctgcaattc ggggttgatt tcggtttgtc cgaggttgtt gacgtagttg
     1381 cacaaagttg ccaaggctac gcccatcacg acttcgactg cctgctgctg gttgtagccc
     1441 gcatcaaaaa atgctttgag ttcctcgtcg gataccgcgc ctttttttgc cattacggct
     1501 tgggtaaagg cggcgagcgc gccgagttta gcatcgtcaa attcgcctgc cgccaaagcg
     1561 cgcgcggctt tgacggattg ttcggaaagg agttttttca gggttgcgag tttggtgtgc
     1621 cctgccacgc agaagccgca ttggttggtg cgggcggcaa taatctggat tacttcgact
     1681 tcgccggcgg tcaggctgtt ggcggcgttg agcttgccga cttcttggta aaacgccaag
     1741 gcttcggggg cgtttgataa tacgccgata aggttgggga taaagccgtt gttttgaagt
     1801 accgcctcga cgcgcgcttt ggcggcttcg ggggcggttt cgagggtgtg tacggttaaa
     1861 cgtgccattt tctttccttg ttcgcaaata ttgggtacgg gcgcatggta tgcatttcgg
     1921 aacggaatag gaaagactga ttggttatgt gctgcaaacg aaaggttata agaaatgccg
     1981 tctgaacatt tttcagacgg catgatggaa aagaaaacgc gcttatcggc ccccgcgccc
     2041 gaaatattgc gccaatgcgg cttgggggtt ggcagcttgg actgtttcgg gcaattcggt
     2101 atagccgcgc gataaaaggt ggcgtgcata aaggctgttg ccctttgcgc cgaaccagac
     2161 ggaaaccgcc aagcccaata cgttaaacgc cgagtctgtg gcgggcgtgt cgccgtaaac
     2221 cagttgggca aacacagcca gcacgaatat cgcgcccgtc agccccagcc ccaaacccca
     2281 cattcttttg aaacacgccc acagcgtgcc gaacaagagc cccggccatg accagccttg
     2341 tttgacggct tggggcggca gggcgggatg ggtgtagatt ttgtatggtt tcatcgtgtt
     2401 tccttttcgg ttgaaaccct gccctttggg aaggtaggat cagactttat agtggattaa
     2461 atttaaacca gtacggcgtt acctcgcctt gccgtactat ctgtactgtc tgcggcttcg
     2521 ttgccttgtc ctgatttaaa tttaatccac tatatttggg aggggcgcgc ccgtgtgtcg
     2581 gcatacggtt tgaaagcgat tacccgacgg ggagcttcaa acccgacaat gccgtctgaa
     2641 cggcgttttg ccttcagacg gcattgcctg ccttcaaagc ggacacgctt attccgccca
     2701 gtttttcttt ttgctggttt tgcctacgcc cgggttgaag ctgttggtcg ggtcaagttt
     2761 gcggtaaaac tgtttgagcg cgggtttggc ttcatacaaa tgaccgacgt tgtgttcggc
     2821 gggatattgc gcgccgcgtt ggtctaaaag atgcagcatt tcgtgttcca acgccatgca
     2881 gtcgttgcct tttttgatga tgtaatcctg atggaaaacg tggcacatga aatgtccgta
     2941 gtagagtttg tggatgattt tattgtcgat ttcgggcggc agtttttcaa accagtcgcg
     3001 gtcgtcgcgg cgcagggcga tgtcaagcgc gaccaagtct tccacttcgt catcatgcac
     3061 ggcgcggtag cggatggcgg cggaggcgac ggcgaaacgg tgcagcatcg cggcttgggt
     3121 ttcttcggcg ttgcactcga aaaacgcgcc gctgtggtgt gcaaaatatt cttttaagaa
     3181 cgcgcgcgcc tcatccacgc ctttcccgcc cattttcaga atcaggtggt gttcgtattt
     3241 gtcgcggtaa tcgcgcatgg atttgggcag gtggtcgggc aggtatttgc tgacgaactg
     3301 catggccttg tcggaaaaat gtttgggcag gaagctggct tttttgccga acctgtccac
     3361 gcgcgccttc aagtcaaata atttcggcag ttggtgcgta ccgaattttt tgatgacgta
     3421 aaacgtgtct ttgccgtaca cgtcggcaat atcgaaagcg tggcggtgga tgtattcgcc
     3481 ggaaacgggc aggctttcaa attcgcccaa ggcggcgcgg cggatgtcgg tcagctcgtt
     3541 gatgtcgttc gtgccgatgt agaacacggc ggtttgtttt tcttgcggga aggtgtccaa
     3601 acggacggca aaaaccatca gtttgcccgc gcagcccgag gcttcgtaat ggcgggcggg
     3661 gtcggcattg aaacgcgcgg cggtcggttc gtccacttgg cggacgtgtt cgcaataggc
     3721 gtggtcgtgt cctttgcccg cgtcttgttt gatgtctttg ttttgataat gatgaccttg
     3781 caggttggtc aggatttctt cgggcgtgtc gcccaagtct atgcccaagt ggttgaccag
     3841 ttccaacctg ccttcttcgt tgatttgggc gaacagtgcc atttcggtgt aggccgggcc
     3901 gcgctgtacc aatgcgccgc cggagttgtt gcacacgccg cccaagacgg acgcaccgat
     3961 gcaggaagag ccgataaccg aatgcggttc gcgtcccaag ggtttcagca gcaattcaag
     4021 ctggttcagg gtcgagccgg gcaggcagac gacttgttcg ttgttgttga tggtttggat
     4081 gatgttcatc cgcatggtgt tcacaatcac gatgtcgcgg tcgtaatcgt tgccgtcggg
     4141 ggtcgaaccg ccggtcagtc cggtattcgc cgcctgcgta atcacaatca cgtctgcttc
     4201 gacgcacgcc tgcagaattt tccacatttc cagaatgctt ccggggcgaa ccaccgccaa
     4261 cgccttaccc tcgccgaagc ggtaaccttg gcggtattgt tcggttttcg cggggtcggt
     4321 aatgatgtat ttttcgccta cggtttgggt cagtcttgac agtaattgtg atgcgctcat
     4381 ggcagtttcc ttaaaattgt cggcaggtgc attgcacatt ggaattgttt tcacattgta
     4441 gttatacgtt atggcaaagt aaagaaaatg ccgtctgaac ggctttcaga cggcaacggt
     4501 gcgatacggg aacgccggaa catcaaagct ccggcgtttc aaatagggcg gcgggccaaa
     4561 cccccggcac tggcgcaccg gagtgggctg ctggcttccg cccctgaccc ggtgttccga
     4621 tttgccatgc ggggagaccc gcctcagaga aacggcatta taacgggttt tctgaaaaac
     4681 tcaaccgttt tgatacggtc atacgccgga aacaccacct aaaatttata tttgataata
     4741 ttgtcaacaa tttctcaaag cgttattttg tttctataag ggtatttcct gtttcggcat
     4801 tgaaaaatat caaaaattga actacattat cgccttttca aactcgcctg aaaccgaatt
     4861 ttcagacggc attcaaataa aaactgccaa gcacggacac accatgacca cgactactgc
     4921 ccctcagcgt attcgagaaa ttccctataa ctatacttct tataccgacc gcgaaatcgt
     4981 catccgcctg ctgggcgacg aagcgtggca gattctgcaa gacttgcgcg gacagcgtaa
     5041 aaccggacgt tcggcacgga tgctgtttga agtgttgggc gatatttggg tagttgtgcg
     5101 caatccatat ctggtcgatg acttgttgga gcacccgaaa cgccgcgccg cgctggtgcg
     5161 ggaaatgcgc caccgtttga acgaaatccg caaacgccgc gacgataatc ggcaagtgga
     5221 tgtattggtt gcggcagcgg aaaaggcagt cgagcgtttt gatagcagtt ttgatgaaac
     5281 cagccaaaaa cgccatcaaa ttttggaacg cttaagcaaa attaccaagc cgcacaatat
     5341 tatgttcgac gggctggcgc gggtaacgca tgttaccgat gcaaccgact ggcgcgtcga
     5401 atatccgttt gtcgtcgtca atcccgacac ggaggcggaa gtcgcgcctt tggtgcgcgc
     5461 cttaattgag ttggacttgg ttattatccc gcgcggcggc ggtacgggtt ataccggcgg
     5521 cgcgattcct ttggacgcaa tgagcgcggt catcaatacg gaaaagctgg acaaacatcg
     5581 cggcgtggaa tttgtcgaac tggcgggctt ggatggcaga catccgatta tccggtgcgg
     5641 cgcgggcgtg gttacgcggc gagtggaaga aaccgcgcat caggcaggtt tggtgttcgc
     5701 cgtcgatccg acttctgccg acgcgtcctg cgtgggcggt aatgtagcga tgaacgcggg
     5761 cggtaaaaaa gccgtgctgt gggggacggc gttggacaac ctcgcctact ggcaaatggt
     5821 caaccctcaa ggcgaatggc tgcgtatcga acgcgtgcgc cacaatttcg gcaaaatcca
     5881 cgacgaagaa accgcaatat ttgacgttca cacactggat tcagacggcc tcaatgtcgt
     5941 caaaaccgaa cgcttggaaa ttcccggtca caaattccgc aaagtcggtt tgggcaaaga
     6001 cgttaccgac aaattcttga gcggcctgcc cggcgtgcag aaagaaggca cggacggcat
     6061 catcaccagc gttgccttcg tgttgcataa aatgccgaaa tacacgcgca ccgtgtgtat
     6121 ggagtttttc ggcacggttg ccaccgccac gccatctatt gtcgaaatcc gcgacttttt
     6181 gcttgcccat gaaagcgtac ggctggcagg tttggaacat ttggactggc gttatgtccg
     6241 cgccgtcggc tacgccacca aagcggcggg caagggacgg ccgaaaatgg ttttgctggc
     6301 ggacgtggtt tcagacgacg aagccgccgt agaggcagcc gccgaacaca tctgcgaact
     6361 tgcccgcgcc cgcgacggcg aaggctttat cgccatatct cccgaagccc gcaaaacctt
     6421 ctggctcgac cgcagccgca ccgccgccat cgccaaacac accaacgcct ttaaaatcaa
     6481 cgaagacgtg gtcatcccac tcgaaaggct cggcgagtat tcagacggca tcgaacgcat
     6541 caacattgag ctttccattc aaaacaaact caaactctgt gccgccttgg agcaatatct
     6601 gtccggcaaa ctccccatcg acaaaatggg aaccgacctg ccgaccgccg aactgttggg
     6661 cgaacgcggc aaacacgccc tggcccacgt ttccgccgtc aaagcgcgtt gggactggtt
     6721 gctcgccaat ctcgacacgc cgcttgccga ctacaaggcc cgctacggcg cagccgtcca
     6781 cgccgcgccc gaagccaaag acaatgaaag ctgctttacc gcattccgcg atttccgcct
     6841 gcgcgtgtct gtcaaagcag acgtaatgaa gccgctttct gaaatcttca gcggcaaaac
     6901 cgacaccaaa atcatcgaag gcttgggcaa aatccacgcc aaaaccgtgc gcggcagagt
     6961 ctttgtcgcc ctgcatatgc acgccggcga cggcaatgtc cacaccaata tccccgtcaa
     7021 ttcagacgat gccgaaatgc ttcagacggc ataccgttct gtggaacgca ttatgaaaat
     7081 cgcccgttcg cttaacggcg tgatttccgg cgaacacggc atcggcatta ccaagctcga
     7141 atttttaagc gacgaagaaa tgcagccgtt ttgggactac aaaaaccaag tcgatcccaa
     7201 acacaccttc aaccgccaca aactgatgaa aggctcggac ctgcgcaacg cctatacgcc
     7261 gtctttcgag cttttgggcg cggaatcgct gattatggaa aaatcagacc tcggcacgat
     7321 tgccgattcc gtcaaagact gcctgcgctg cggcaaatgc aaacccgtct gctccaccca
     7381 cgttccgcgt gccaacctgc tgtacagccc gcgcaacaaa atcctcggcg tggggctctt
     7441 gaccgaagcc tttttatacg aagaacaaac ccgtcgcggc gtttccgtca aacattttga
     7501 agaactggca gacatcggcg accactgcac cgtgtgccac cgctgcgtca aaccctgccc
     7561 cgtcaatatc gacttcggcg acgtaaccgt agccgtccgc aactaccttg ccgattccgg
     7621 ccacaaacgc tttgcgccgg cagcttcaat gggtatggca ttcctaaacg cgaccggccc
     7681 gaaaaccatt aaaaccctac gtgccgcgat gatacaaacc ggcttccccg cgcagaattt
     7741 cgcctacaaa atcggcaaac tgctgcccgt cggcacgaaa aagcaaaaag ccgaacccaa
     7801 ggcaaccgtc ggcaaagccc cgattaaaga acaaattatc cacttcatca accgtccgct
     7861 gcccaaaaac gtgcctgcca aaacaccgcg ctccttattg ggcatcgaag acgacaaaag
     7921 catccccatc atccgcaatc cggcttctcc ggaagatgcc gaagccgtgt tttactttcc
     7981 cggctgtggt tcggaacgcc tgttcagcca aatcggactc gccgtccaag ccatgctctg
     8041 gcacgtcggc gtgcaaaccg tcctgccgcc cggctatatg tgttgcggct atccgcagga
     8101 cgccggcggc aataaggcaa aagccgaaga aatgagcacc aacaaccgcg tggctttcca
     8161 ccgtatggca aacaccctca actacctcga catcaaaacc gtcgtcgtca gttgcggtac
     8221 ctgctacgac cagctcgaaa aataccgctt cgaagaaatc ttccccggct gccgcatcat
     8281 cgacatccac gaatacctgc tcgaaaaagg cgtgaaactg aacggcgtga aaggtcagca
     8341 atacctctac cacgacccct gccacacgcc catcaaaacc atgaacgcca cccaaatggc
     8401 cagcagcctg atggggcaga aagtcgtctt aagcgaccgc tgctgcggcg aatccggcat
     8461 gtttgccgtc aaacgccccg acatcgccac ccaagtcaaa ttccgcaaac aagaggaaat
     8521 tgagaaaaac ctcaaagagc tgccgcaggg cgaacccgtc aaaatgctga cctcctgtcc
     8581 ggcgtgtctg caaggcttga gccgctacgc cgacgacaac aatatgccca ccgactacat
     8641 cgttatcgaa atggcgaaac acatcctcgg cgaaaactgg ctagacgagt ttgtgaaaaa
     8701 agccaataac ggcggcgtgg aaaaagtatt gctgtaacaa cggacacgga aatgccgtct
     8761 gaacgccgaa gtcttcagac ggcattgttt aaacccaata cctacccaaa tcatgattga
     8821 cggacaaaca accgaaccca aacaaaaaac ccgaatcatc cttgccccta tgcagggtct
     8881 ggtcgatgac gtgatgcgcg acctgctgac gcgtattggc ggctacgacg aatgcgtcag
     8941 cgaatttgta cgcattaccc ataccgtgca ttcccgatcc atatggttaa aatatgtccc
     9001 cgaaatcgcc aacggaaaca aaacgttttc cggcacgccc tgcaccgtcc aacttttggg
     9061 cagcgatgcg gacaatatgg cggcgaatgc gctggaagcc gtccgcttcg gtgcgaacaa
     9121 aatcgatttg aacttcggct gccccgcgcc caccgtcaac aaacacaaag gcggcgcaat
     9181 ccttttaaaa gagccggaac tgatattcca catcgtcaaa acgctgcgcg gacgtttgcc
     9241 cgcacatatt ccgctcaccg caaaaatgcg gctcggttac gaagacaaaa gccgggcttt
     9301 ggaatgcgcc tgtgcgattg ccgaaggggg cgcatgcgga ctgaccgtac acgcgcgtac
     9361 caaagccgag ggttacgaac cgccggcgca ttgggaatgg ataaggaaaa tccgagacag
     9421 cgtcaatatt cccgttaccg ccaacggcga cgttttcagc ctgcaagact atatcggcat
     9481 caaaacaatc agcggctgca acagcgtgat gctcggtcgc ggcgcggtca tccgccccga
     9541 tttggcgcgg caaatcaagc aatacgagaa cggcgggccg gtcaaagaca cggattttgc
     9601 cgaagtttcc aaatggatac ggcagttttt cgagctgtgc ctgacaaaag aggcaaacaa
     9661 taaatacccg atcgcccgtc ttaagcaatg gttgggcatg atgaaaaaaa cgttcgaccc
     9721 ggcgcaaacg ctgtttgacc gcgtccgaac ggttaaggat gcggatgaag tccgccgaat
     9781 cctgaacgca tttgagcatg agataaatgt ttgactgtct cccatgcttc aaagcaaatg
     9841 ccgtctgaaa atctttcaga cggcatttca tatctttatt gctgcttttc ttccgtatcc
     9901 ggcgtatttt tgtttttcag cttcgcaccc aagcccaaac gtttttcata atccgattgc
     9961 ggggtatcgt cttcctgcat accgaatgcg ccgacattga cccacagcga cagcagcgcg
    10021 acgacaaagg agcaaaagcc gattacatac caaaacattg cccctcccga tttgttaaaa
    10081 tcatatcaaa tacagtgccg aatttatcac aaacgcacgg gcaaatatag tgaattaaat
    10141 ttaaataagg acaaggcggc gagccgaaga cagtacaaat agagaccttt gcaaaattcc
    10201 ccaaaatccc ctaaattccc accaagccat ttaggggatt ttccatgagc accttcttcc
    10261 ggcaaaccgc
//
LOCUS       DAWGJV010000064        10249 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWGJV010000064 DAWGJV010000000
VERSION     DAWGJV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.064"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            69..2183
                     /locus_tag="WHQ94_001183"
     CDS             69..2183
                     /locus_tag="WHQ94_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ2128460.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLFTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVNLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPKIDSLVQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2320..3579
                     /locus_tag="WHQ94_001184"
     CDS             2320..3579
                     /locus_tag="WHQ94_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ2128461.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3946..5352
                     /locus_tag="WHQ94_001185"
     CDS             3946..5352
                     /locus_tag="WHQ94_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ2128462.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAEFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5570..6784
                     /gene="gltS"
                     /locus_tag="WHQ94_001186"
     CDS             5570..6784
                     /gene="gltS"
                     /locus_tag="WHQ94_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ2128463.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6883..7899
                     /locus_tag="WHQ94_001187"
     CDS             6883..7899
                     /locus_tag="WHQ94_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ2128464.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAGEDAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7911..8108
                     /locus_tag="WHQ94_001188"
     CDS             7911..8108
                     /locus_tag="WHQ94_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128465.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8126..8275
                     /locus_tag="WHQ94_001189"
     CDS             8126..8275
                     /locus_tag="WHQ94_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128466.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHRTIQKAS
                     PYVPS"
     gene            9848..10141
                     /locus_tag="WHQ94_001190"
     CDS             9848..10141
                     /locus_tag="WHQ94_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128467.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT         2639 a         2951 c         2371 g         2288 t
ORIGIN      
        1 aaagacttgg caggcaaagt attggccgaa gcagtcacgt tttaagacta ttcaggccgt
       61 ctgaaagaat gttcctcttt tcagacggcc tacaatccat taataacaat aatcgacgaa
      121 aacgcattgt gaaaaacgcc tacatcccct ctcgtggcat ccgcaaaatc ccccatctct
      181 tcaccctatt gcctgagttt catatctgca aggatgggaa aggagcagag gctgttatcg
      241 gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccgctgaa caccagcttc
      301 cctttattgc tttggaagac ggctttttac gctcgcttgg cctgggcgtt gccggctatc
      361 ctccctattc catcgtctat gacgacatcg gcatctacta cgacaccaca cgtccttcgc
      421 gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg gctcaggcgc
      481 agcaggcgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac gcgcccgaac
      541 tttcagacga tcatccttta cgctccccat ccaaacctga aaccgtcctc attatcgacc
      601 aaacattcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg tttgaactga
      661 tgtttcagac ggccttaaat gaaaacccgc aagccgatat ttgggtcaaa acccatcccg
      721 atgttttgtg cggcaaaaaa caaggctatc tgacccaact ggcgcagcaa caccgcgtca
      781 atcttctggc agaagacatc aatccgattt ctttgttgca aaacgtcgat aaagtttatt
      841 gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg gtaacgtttg
      901 gtttgccgtg gtatgccgga tggggcgtga gtgacgaccg ccatcctaaa atcgacagcc
      961 ttgttcaaac ccaacgccgc gccccccgca acttgctgca gctcttcgct gcagcctatc
     1021 tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc tttgatgtca
     1081 tcgactatct ggcgacggtc aaacgcaaaa acgacaaatt gcgtggcgag ttgtattgcg
     1141 ttggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta ccctcttgcc
     1201 gtctgaaatt tatctcttcc acccaaaaac tggcaggggt caaactgtcc gacgatgcac
     1261 gcatccttgc ctggggcaac ggcaaagagg ccatcgtccg ctttgccgaa caacaccaca
     1321 tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc tccaacttag
     1381 tgccgccgct gtcgctcgtt accgacgata tgagcattta tttcaatgcc gaaaccccgt
     1441 cccgtcttga atacatccta caaaaccaaa acttcgacga tcaagacttt cagacggcct
     1501 tgaagctgca aaaaatgctg accgaaaacc acatcagtaa atacaacgtc ggcagctcag
     1561 acttcaccgc cccgtcaacc gacaaaaccg tgatcctcgt tcccggccag gttgaagatg
     1621 atgcgtctat ccgctacggt tcgccccaaa tctaccgcaa tctggatttg ctccgtaccg
     1681 tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat gtagtcagcg
     1741 gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac caaaccgccg
     1801 aacaagccga catcctgacc tgtctccaat acgcagacga agtacatacc atgacttcgc
     1861 tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc ctgccttttt
     1921 acgcaggctg gggacttacc caagatctgc tccccattcc gcgccgtagc cgcagacttg
     1981 agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc caccccgaaa
     2041 cccatcaggc cataaatgca gaaaccgcag cccaaatcct gatacgacaa aaaaatatgc
     2101 aaaaaaacaa caacggatta catcgcgggt actttgccaa aaaattaggt aaaatcaaac
     2161 aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat aaacttgcct
     2221 ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt gaaagttcaa
     2281 cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac cgtcctcaaa
     2341 aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca aggccctgtc
     2401 ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac cgtacataaa
     2461 ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca taccgttgtt
     2521 tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac tcaacatcac
     2581 atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc aaaacgcatt
     2641 gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg cccctactac
     2701 atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg tgccgacttt
     2761 tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac gccggtacac
     2821 ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt gttccgcaat
     2881 ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca ttacctcaaa
     2941 ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat ccaaatcgcc
     3001 aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca ggtattcaac
     3061 gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct gctccatgtt
     3121 ttgagttcat ttgccgagca cgcgcctgcc gataccaaca tcatcatcaa gcatcatccg
     3181 atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa agaacacccc
     3241 gaactcaaag gccgcgtgat ttatgtccat gatgtccccc tgcccgtttt cctgcgccac
     3301 ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggactgat tcacaatatg
     3361 ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga ccaaaatacc
     3421 ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca tgcctaccga
     3481 atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt gtttttcccc
     3541 aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg aagttcaggc
     3601 cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg ccatgtaaat
     3661 aaaacacaaa aatctgcatt tatcattaac aataaattac aaaaacagta taatgaccga
     3721 gctgccatga gcgcataccg actcaagctg aggccttaat aacacacaaa atatggatgt
     3781 actcccaggc aaaacaatat aacaagccaa acatcctaaa gataagccag caaggcaata
     3841 cactttacaa aactgtggcg agcaatattt ttacaaagcc ctcaaccggt atcgccgccc
     3901 atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac cgccgctcct
     3961 gatatcctct accggcaagc cgccgccctt ttggaacaat ccaataccgc ccaagccctg
     4021 cccctgttgc aacaggcggc agagcaaggt tatgcggaag ctgctttcgt attgggcaac
     4081 catctgctgc aaaacggcca accggagcag gcactttcat ggttggaagc cgccgcggcc
     4141 caacgccatc ccaaagcact cttctccctg ctgcaacaac gcgaacacaa cggcaccccg
     4201 accggacagc ttctcaacga ctatgcctgg ctgggtgagc aggggcactc agaagcccaa
     4261 ttaatcctca tgcgttacca cgcgcaacgc aacgatccac aatcgctcta ctgggcggaa
     4321 cttgctgccg cccgatatgc cgcacctgcg tattaccatc tggcacgcca tcatcaacgc
     4381 caaggcgacg ttgaaacagc catcgaacaa tacgaaaaag cggcagcact cggcgtaact
     4441 gccgcctgct ggcaacttgg tcaaatctac ttctacggta caggtgtcag ccccaaccac
     4501 gcacaagccg aacactatct cgaaccagcc gcacaagccg gccacatcgc cgcacaaacc
     4561 ctgctggccg accttcttgc cgcccaacgc aaacccgaag ccttagaatg gtatcgtcgt
     4621 gccgccaata aggagcaagc ggaagcacaa gccaagctgg cccaatacgc cctgaccggc
     4681 gaactttccg aacgcgatcc gttccaagcg gcgcgctatg ccaaagccgc tgccgagaaa
     4741 aaccatcctg aagccctgaa aatcatgggc gacctctacc gctacggcct cggcatcaaa
     4801 gctgacaacc atatcgcgca cgattactac caccgtgctg ccgcgctggg ttctgccgcc
     4861 gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata cgaacaaatc
     4921 aaaactgccg ccctgcaaca gcaacaaacc gaaaccatct accgtttggc ggaaacacaa
     4981 gcccatgcca tcggtcgtcc cgccgactat aatgccgcac gcaaaaatta catggaagct
     5041 gctgagttcc accataaaaa cgcggcggct gccttaggcc gcatctacca ttacggcctc
     5101 ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccatcgctgc cgaacaaaac
     5161 cacccttccg cccaatacca cctcgcctgt ttttactatc acggacaagg tgtcggctgt
     5221 catgttccga ccgcctgcta ctggctgcag gctgccatca gcaacggcca cacttcggcc
     5281 gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc catcggacaa
     5341 aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta ttcccctaat
     5401 atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga aaacaccttg
     5461 tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac acactacatc
     5521 cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca tggaatggga
     5581 attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg gtaaatttct
     5641 ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtcg ctggcggttt
     5701 ggttgccgcg attatcctgt ttgcgctgca cgaggcgtac ggcgtgagct tcaaatttga
     5761 gaaaccgctg caaaatgcgt ttatgctgat tttcttcacg tccatcggct tgagcgcgga
     5821 tttttcccgt ttgaaggcgg gcggtttgcc gctagtggtt tttaccgcga ttgtgggcgg
     5881 atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg gtttggaccc
     5941 gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta cgtcaggtgc
     6001 gtggggacct aattttgaaa cgcaatacgg cttggtcggc gcaaccggtt tgggtattgc
     6061 atcggctact ttcgggctgg tgttcggcgg cctgatcggt gggccggttg cgcgccgcct
     6121 gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg acgacaacgc
     6181 ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat ctgccgttga
     6241 aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg gcttcgacaa
     6301 agaatatctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg tggtcatccg
     6361 caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg atgtgttcgg
     6421 caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac tgtgggagct
     6481 gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg tgatggtttt
     6541 gtacgcgact tttgttacct atgtctttat ggggcgcgac tatgatgcgg cagtattggc
     6601 tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa atatgcagtc
     6661 cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta tggtcggcgc
     6721 gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt tctttaaagg
     6781 ctgattttcc gcctttccga caaagcacct gcaaggttta ccgcctgcag gtgcttttgc
     6841 tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg gaaactcatt
     6901 gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc caaggcattg
     6961 gaatgcgcca accccgccgt gttgcaaggc atacgcggca gtattcagga aacgctcacg
     7021 caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc cgacaaaatt
     7081 atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac gcaggaaggc
     7141 gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac gcttgccgat
     7201 gccaaggcaa acagccccct gctgtacggg gaaaccgctt tgtcggatat tgtgcggcag
     7261 aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt ccgcttccta
     7321 cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc ggcgcaaacg
     7381 ctgtctgccg cgttgctgcc ttacggcgtg aagagcatcg tgatgataga cggcaaggcg
     7441 gtaaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga agaaccgtcc
     7501 aaacccacgc ccgaagacat tttggaacat aatgccgccg gaggggatgc agacgtaccc
     7561 caagccggag aagacgcgcc cgaaccggaa atcctgcatc ccgacgacgg cgagcgtgcc
     7621 gataccgtta ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa ccagcgtgcg
     7681 gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa agagttggtc
     7741 ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc cgccgcgcag
     7801 gcagaccggc aggaatacgc cgaatacctc aagctgcagt gcgacacgcg gatgacgcgc
     7861 gaacggatac agtatcttcg cggctattcc atcgattagg gggcaaaccg atgactaccg
     7921 tccccaaaag caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag
     7981 tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg
     8041 ccagattggg cgagaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg
     8101 gcagataaag ggaaaattga cggaaatgcc gtctgaaacc ctgttatcgg tttcagacgg
     8161 cattttgact aatacggtac gcaggcgcaa aacagccgtc ttttcctgcg tttcctatgc
     8221 tgatgtttca acacacagga cgatacaaaa agcgtcgccc tatgttccgt cctaattttt
     8281 gttaatccgc tctaaagtct gatcctgccg ccctaaaggg tgggatttca accgaaaagg
     8341 aaatacgata gaatcggcaa tttgccgtaa agttaaaaag ttccatcata tcgaggagac
     8401 attatgcatc accccccccc cgcaaaatga acatttcgag caagacctta tcccttacgc
     8461 cgtccgtttc aggctcaatc aaatccttcc cgaactcgac tgccattggc aggaaaatct
     8521 gacagaaatc ttcaaaacac tttctcccga agccgggcag gccgcctgta cgcaaatcct
     8581 ttcccgaaaa ggcataggct ataacgaagc cgacggttct tttacttacc ttccccaaaa
     8641 acgcatttcg ttttcttctt tggcaacaga cattcccatc cccgacctca aacggctggc
     8701 ggggcatatt gcggaacgcc tggatgtatt ggcgcaatgc gaatcgccgg tttatattgc
     8761 cgacattctc gaaagcgtgc tgtccgttat caaaaacgtc gataccggag gcaagcgcgc
     8821 acgggaaaat ttcaaacaga ttctgaaaac cgaattcatc tacgccgccg ctgcggtatt
     8881 gaaaaacaaa accaaatggg aaatccccga aaatacccgc aatctcgatt cggacatcat
     8941 ctgtacctat atctgcgaag tgttcctaaa atcgcacctg ctcggcaaca gttttccgat
     9001 gatgcgcccg cgcgaagtga agcagatgcc cgaaccggtg tttgacaaag tattgtttgc
     9061 cgaacagcgc gcccgccagt tggaagttat ccgaacgtca aaatacatct tcgccatcgc
     9121 gccttattac accgacgata ttccgttttc cctgcgccgc ttcctgtcgg aagacaaact
     9181 ttataccagc tacaaccgct attacaccgc catccatatc cccgtccgca acatcactca
     9241 gaatgatgcc ctgcgttttg ccaacggttt gaaacagatt ctctcgctgc aatccggcgt
     9301 gagctgggaa atcatcgata tgatggacag gatagaggaa aactacgaca aaaaagccct
     9361 gccgctgttg ttttcgccat tccccgcgca agtcgaacgc acgcaggcga ttgccgccag
     9421 attggaccaa ttcgagcggc tgttgggcga caccgtcctc gatccgttct actattgcct
     9481 gacgcgtatg gcgaagggcg aggaagattt gaaatatatt tatatcgcct tccgccagtc
     9541 tttcggcggc atcttcaatt cgtttgaaaa cttccgcctg ctgcccgcgc tgtggatgag
     9601 cagggatgcg gaaaatatgt ccgaccgcat gaacgcctat atttccgcaa tggaagaccg
     9661 ccgccgcgag atattgtcca ttcggcaggg aaagcctgaa gaagaatttt cccgcaagat
     9721 ggtcgtctgc ctgatcgact tggaaaattg tttggaaaaa cacctcgaac agttcaagcc
     9781 ggtatcggaa agcatcgaag cctgtaccgc caaactgcag gagcaaccgt cgttcttcaa
     9841 caggctgatg aaaaccaacg acaagctcaa caggcagatt gacgtgttgc agaaacagtc
     9901 ggcagccatc cacaacgaag cctatatcga aatgaacacg ctgctttacc gccaccgcga
     9961 agtggtttcc gtccacaacc gcaaagcaga ttatgcggaa aagggcaaag agcagattgc
    10021 gctgttcccg cgcggattga acggcatcac caagctgccc gccgccgtcc tgttgcccga
    10081 gcgtccctac cattttgata tgaaggaggt tctgcatata ttctcctgga taccgaggta
    10141 ggcgtatcgg ctgccggata aaaatgcacc ccgtcattcc tgtctgtacg ggggtagcgg
    10201 cagtgttttc ctgcttcgtt ttatagtgga ttaacaaaaa ccagtacgg
//
LOCUS       DAWGJV010000065        10228 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWGJV010000065 DAWGJV010000000
VERSION     DAWGJV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.065"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(116..2122)
                     /locus_tag="WHQ94_001191"
     CDS             complement(116..2122)
                     /locus_tag="WHQ94_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ2128468.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLTIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2411..4438
                     /locus_tag="WHQ94_001192"
     CDS             2411..4438
                     /locus_tag="WHQ94_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ2128469.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4530..6083)
                     /locus_tag="WHQ94_001193"
     CDS             complement(4530..6083)
                     /locus_tag="WHQ94_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ2128470.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVTLAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(6332..7525)
                     /locus_tag="WHQ94_001194"
     CDS             complement(6332..7525)
                     /locus_tag="WHQ94_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ2128471.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDTLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLIEVQQMQRRVA
                     RQGQTMFWSWQNAMGGVCSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRS
                     TAIRQ"
     gene            complement(7522..8508)
                     /locus_tag="WHQ94_001195"
     CDS             complement(7522..8508)
                     /locus_tag="WHQ94_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ2128472.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPPEAAASEAVPQTGEI
                     QEWKQNTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKHYLKFAADEWAQEYLKRTDR
                     ILEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGED
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(8519..9955)
                     /locus_tag="WHQ94_001196"
     CDS             complement(8519..9955)
                     /locus_tag="WHQ94_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ2128473.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(10085..>10228)
                     /locus_tag="WHQ94_001197"
     CDS             complement(10085..>10228)
                     /locus_tag="WHQ94_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ2128474.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT         2113 a         2466 c         3197 g         2452 t
ORIGIN      
        1 taaaatgccg tctgaaccac gtttcggttc agacggcatt tttgcatcct tttatgggga
       61 ttttccgcat cagggcggta ttcggggaga agcccgcttc agacggcatt gccggtcaag
      121 gcttgtccga aggaggatgt ccctgttctt tggcgggcgg gaggaaaagt atgcagggat
      181 tgcttttaat ctgattccaa aaactcttaa tcagattgcg gatgctgccg attttcgtgc
      241 cgcgcgagcg cagggcgacg aagagggcga ggctgaaact gacgcagagg ttgaccaggc
      301 cgatggtgag gacgctgaaa atgccgagta cgaacgtgcc caaaccgacg ttgccgctga
      361 cggcggcata gccgaggttg gcggaggaaa aggcgacgtg gcggatgtcc agcggcagcc
      421 cgaggaggtg tccgaaatag ccggtcatac ccaagagcat cccgaagatg aagttgccta
      481 ccaacgagcc gtagtgtttg tggatgtagg cggcgaggac gcggcggggc ccggggcgca
      541 tgattttacg cagcaagggg ttgaagggca ggcgttggcg caggttgagg tagtcggcgc
      601 ggttgtcgaa aaaacctgcg atgatgccgg agcagaacag ccacagacct gcaatggcgg
      661 cataccacag cgtcgggtaa gcgatgatgt ctatggattt gaactggtag gcggcggtgt
      721 gggcatcgag tatgggctgt tggtgcagat gggcatagcc gaacgatatg gcgcacgcta
      781 aaaggatggc gatggaaacg ttgccgaaga cagcgacgct ttgggagcgg catacgtcga
      841 tgaggagctt ggcgagcttg ttgtccaccg ctttgccgcc ttcgttgaga tcgacctgtt
      901 cggcaaagct ggcggcagtc atcgcgggct gtttggtggc gacggtgcag tgcagcatat
      961 ggatgatcat aaagccgatg ccgtagttga acccagccga caaggaagtc aggaaggggc
     1021 tgaggccgag tgtgccgatg cggattttga gcagcgccat cagggcgatg atgatgccgc
     1081 cgcctgccgc cgagtagaac attttgaaat attctttgcg gttgcgggtg atgtagtgtt
     1141 cgccgtggtg gcttttgttt tcggtaatgc tgcgggcgag catacggatg ctgctgcggc
     1201 gcaggtggcg ggtgctgtat tgttcgaccg ccgcgtagat gagggagttc atcagcgcga
     1261 tggtcaggcg gttgctgttg ccggcaccgg tttggatgtc ggtgagcagc tttagacggt
     1321 ctacggtctg ccggagccgt tcgagcaggt gggcgacttt gaccgacgaa ccggagccgg
     1381 cgcccgtccc tttgcggcgc aaatagtcaa tctggctgaa acattggtcg aacatcactt
     1441 cgaggtgggc ggtgtcgtaa ggcgcggtgc cgttgcggta gtgttcgacc agtttggcgg
     1501 tttcgcgttg gagggcgacg aaggaagaat cggcttccag caggcgcggg gcgatgcgga
     1561 tgaggtcggg ttcgatggct tcggatgccg tccagacaga cagcatttcg atggcgcgca
     1621 aacgcgcatc ggcaaggcgg atgcccgccg tctgcaattt ggacggctcg gcgtggctcc
     1681 ggatgagttc gtataaaacc agccattggc ggatattgag cgtttgcagc catttgtcgt
     1741 cgttttcgga atggaaaaga taaaggaaga cttcgcccaa gttggcaaaa tctttatatg
     1801 acgggctgaa gcgttcgtat atgcgtatgc ccatttcccg ggcaaagctg tggcgcgaga
     1861 agatgccgag tttgatgagt gccggataaa tgtgtatttg cgcgagccag atgtaaaaac
     1921 accggctgaa gcgggacagc aattccctgt cttgtttgaa tgtgtcgata atcaggtcga
     1981 aacgttcccc cgcacatttt ttgccgccac ggcgcaaaaa tttaatcagt gcgttgagga
     2041 cgttgacaaa atcggtatgt cccaagcttt ccgaaagcag ggggcgcagg ttttgagggg
     2101 taattttctt catatcagcc attgtaccgc atcggcaatc cggcgttaaa cgccccgata
     2161 gcggggaggg cgcgcctgcg gtgggcggcg cattttccgc cggtttgaaa tcaaactata
     2221 ttaatgatat aataaatatc gataaatcaa tctgatagaa taatccgtat tgatttttaa
     2281 ttttttcgat tataacaatt tcggccggtt tgtgcatttc gtcatccgga agccggtatt
     2341 ttaccggcag gttttgaccg ctttgcccga tgaccgggtt tgtctgtttt taacaaagga
     2401 gtgagaaggt ttgtccctgt ctgaatttat agaacgccga acgtcattta atccgatggt
     2461 tattttgacg actttgtttt ttgtgtgtgt tttggtggta ttggttttaa ccgtgccgga
     2521 tcaggtgcag atgtggctcg atcgggcaaa agaagtcatt tttaccgagt tcagctggtt
     2581 ttatgtttta acgttttcca tttttctggg tttcctgctg atactctcgg tcagcagttt
     2641 gggaaacatc aggctcggac gggatgaaga tgtgccggaa ttcggcttcc tgtcgtggct
     2701 ggcgatgctg tttgcggccg ggatgggcgt gggcctgatg tttttcggcg tggcagagcc
     2761 gttgatgcat tatttttcgg acattacggc cggcacgccg gaacacaggc agcagcaggc
     2821 attgctgcac acggtgtttc attggggcgt tcacgcttgg tcggtgtacg gtacgattgc
     2881 attggctttg gcttatttcg gtttccgcta caagctgccg cttgccctgc gttcttgttt
     2941 ttaccccctg ttgaaagaaa aaatttccgg aaggttcggc gatgccattg atattatggc
     3001 gttgcttgct acttttttcg gcatcatcac tacattgggg ttcggggctt cgcaactggg
     3061 cgccggattg caggaaatgg gctggattgc cgaaaacagc ttcagcgtgc aggttttgat
     3121 tatcgccgcc gtcatgtccc tcgccgtcgt ttcggcaata tccggcgtgg ggaagggcgt
     3181 gaaggtgttg agcgagttga acctgagcct tgcgtttttg ctgctgtttt ttgttttggc
     3241 ggcgggtccc actgtttacc tgttgtcggc attcggcgac aacataggga actacctcgg
     3301 aaatctggtg cgcctcagtt ttaaaactta tgcgtacgaa cgggaacaca agccgtggtt
     3361 tgaatcttgg acggtgcttt attgggcgtg gtggtgttct tgggcgccgt ttgtgggttt
     3421 gtttatcgcg cgcatttcaa aggggcgcac catccgcgag tttgtcttcg gggttttgct
     3481 catccccggc ctgttcggcg ttttgtggtt taccgtcttc ggcaatacgg cgatttggct
     3541 gaatgacggg gttgcggggg gaatgctcga aaagatgacc tcctctccgg aaacgctgct
     3601 ttttaaattc tttaattacc tccccctgcc cgaattgacg agcatcgtca gcctgctggt
     3661 catttccctg ttttttgtaa cttctgccga ttccgggatt tatgtcctga acaatattac
     3721 ctctcgggac aagggcttga gcgcaccacg gtggcaggcg gttatgtggg gcgtgctgat
     3781 gtctgccgtt gccgttttgc tgatgcgctc gggcgggcta ggcaacctgc agtctatgac
     3841 cctgattgtt tccctgccgt ttgccctgct gatgctgata atgtgtttca gcctgtggaa
     3901 aggcttgagt gcggataaga aatattttga gacccgggtt aaccctacca gtgtattttg
     3961 gacgggcggc aagtggaaag aacggctggt gcagataatg agccagacgc aggagcagga
     4021 tattttaaaa ttcctcaaac ataccgcatc gcccgctatg cacgagttgc aacgggagct
     4081 ttcggaagaa tacggcttga gcgtccgggt cgataagatg tttcatcagg acgagcccgc
     4141 aatcgagttc gtcattcgga aagagacgat gcgcgatttt atgtacggga ttaagtctgt
     4201 cgggcaggat gtatccgacc agttgattaa cgacggcaag ctgccgcata tccggcacca
     4261 gacaacttac aaaccttacg cttatttttt cgacgggcgc gtcgggtacg atgtgcagta
     4321 tatgaacaag gacgagctga ttgccgacat tttgaaaaac tacgaacgtt atttgatgtt
     4381 gttggatgat gtcggtcagg aactgatggc gcacgagcag gtggaattgg cagagtaagt
     4441 gccttatctc cggcgtttgc caaaaaaaat gcggtgaatc agattcaccg catttttttt
     4501 gccggacggg gcttcggacg gcacggcgtt catttgtttc cgtcaaactg ctccttcaac
     4561 acccgtttca ataccttgcc cgtagcgttg cgcggcagcc cgtctttaaa gtggatttgt
     4621 ttggggattt tgaaatttgc cagcacggta cgcaggtggc ggcggatttc gttctcgccc
     4681 aaatccatac cttccttcaa ttggacgaag gcgacgattt cctcgtcggc ataacggtct
     4741 ttcacgccga tgacggcagc ggcttcgacg gcatcgagtt tgtagatttc ttcttcgatt
     4801 tcgcgcggat agacattttg acctttggaa ataatcaaat cttttttgcg gtcgacgata
     4861 aagataaagc cgtcttcgtc tatggtaacg aaatcgcccg ttttcaacca gccgttgacg
     4921 atggtttcat cggtggcggc aggcatattg aggtagcccc gcatcaccga accgcccctg
     4981 acaatcagtt cgcccacttc gccgcgcggc acttcgacca gttcttcatc gacggctttg
     5041 acttccaaac cgggcagggg gatgccgacg ctgcgggctt tttgcctctc gggcgtattg
     5101 acggcgacga cgggtgaggc ttcgctcagt ccgtagcctt ccagcaattt ggcgcggggg
     5161 aacttggctt tgaaatcgag gatggtttgt tccgccaaag gcgcaccgcc gctgataaac
     5221 aggcggatgc ggttgaacca tctgaaatac caagggattt tcgccttgct catcgcggtg
     5281 taaatcgcgg gtacgcccaa aaacacggtc gcgcgtttga gcagggcctg tttcaaaacg
     5341 ttggaaaacg ggaaaacgga tttgaccaaa ataatcgaac acgccatata aatcggcagc
     5401 agcaccatag ccgtcagcgt gaagctgtgg aacatcggca ggaaaacgat aaagcggtcg
     5461 cgcttggaga ttttaaagat gcgttcgatg ccgttcaggt tggcgaacag gttggcgtaa
     5521 ctgattagcg cgcctttggg atgccccgtc gtgccggagg tgtagatgat gtgtgccaaa
     5581 tcatttatcc ggggttgtcg gcccaagtcg ggtttttcgg ggaagcggcg cacgtcttca
     5641 aaaaaggcat cgccttccgc catttcgccg gtcggacggc ttttgtccgt ccaaatgatt
     5701 ttttcgacgg gcgtttgcgc cttcaagccc gccaattctt ttgacaggcc ggccgaggcg
     5761 aacaggaagc gcgccttgca gtcgttcagg atatacgcgt attcgctgtt tttcaaaaat
     5821 gtgttcatcg gtacggcgac cgcgccgatg gcggagatgg cgaaataggc ggtaataaat
     5881 tctgtggaat tggaaaccgc cagcgtgacc gtgtcgccga acttcacgcc gatattttgc
     5941 agatacgccg caacggcttc ggcctcctgc ttgagcgcgc ggtaggcggt tttttccttg
     6001 ccgtcgaaca ctgccgtgcc gttgccgttt ttgcggcagg cggcggcgag catttcgtag
     6061 aaattggcat aagtccggtt catgaaactt cctttatggt tgcggacggc gaacgccctc
     6121 atttgaaaac aatatgccgt atgaaaacgg aaggcggcag tttatcatct ttgccttcag
     6181 acggcatatc aaacgccgcc tttgcgccgt atgccggagg ttcgcccccg tccttcttat
     6241 gtctgacctg aatgccgtct gaagcaatgt gaaggtatgc gtttcagacg gcatccccct
     6301 gttgcggaaa taccgtccaa accctgtccg attattgcct gattgcagtg gagcggacga
     6361 gttcttcgag gctgtcggcg agcatttccg ccgcgcgccg gtagcctttg gcggagaagt
     6421 gtacgccgtc tttggcggcc catccttggt tgagccagtt tttcatgctg caaacgccgc
     6481 ccatcgcgtt ttgccaagac caaaacatcg tctgcccctg acgggcgacg cgccgctgca
     6541 tctgttggac ttcgatcagg cggacggggc gcgtgccgca tacgccgagc gtgtttttca
     6601 gggattcggg cgcgccgatg atgaggatgc cggcggcagg caggctgtcg cggatttggc
     6661 ggacggtatc cagccatttt tgttcggtgt cggcaatgtc gatgttgtcg ccgaaggctt
     6721 cgttggtgcc gtaggaaagg ataaccaaat cggcgccggt ttgggcaagg tcgttcatac
     6781 ggtcggcacg ccatttcgac caatgggtca attgtgcgcc gttgatgccc atggcggaaa
     6841 cggtaatgcc gccggcggga ttttcgatgt tgatgaagcc gatgtcccac ggcatttcgg
     6901 tctgtatggt caagggcagt gccgcgcggg tatccagtac ctgccagccg ccgccgttgg
     6961 cggagacggt gttgccgttg acggtcaggg tttgttcggc gagcaggggt ttggcaaaca
     7021 gggaaatgcg ctgtttgccg gtcttgccgt ccgatgcggt cagggtcatg ctgccgccgc
     7081 tgccggtgtg ggcgaggatg ccgccgagcg ggaagtctcc gctattgttc ctgctggtga
     7141 ggctttgcca gttaccgttg tgccggacgg ccgccatgcg ctgccctttg acgttggcgg
     7201 ggtaaaccca gcctatgccg ccgtcgcccc aagttttttg caggcgtttg cgcagggtgt
     7261 cggtaaagaa gtcgccggcg gtatgtgagt cgccgatttg caggatgcgg aaggtctcgc
     7321 cgctgccttg tgcgacggat cggagttttt tcatccaagg cgaggcggcg gcgttgccgt
     7381 agtcggtcag cagcccgttt gtatcggtgt agggggcgga cggggaaacg gtaacggtgt
     7441 cgaggctgac ggaggcgaca ggcagggctt ctgcctgcgt ggcggcgaac agggcggaaa
     7501 atgcgatgag gtgtttgggg ttcatggctg tgtacttgat ggttgcgtac ttggttcaaa
     7561 aacgattttt tccattattt ttgccgccag cagtttttgt ccttcggcgg taaagtgtat
     7621 gccgtccttg ctgcggtagc ggacgggttt gccgttgacg ttgacggaat cggtgtagcg
     7681 gtcttccccg ccgctcaggg tgtgcgcggt gggaatcagg atgattttgc cttccaagtg
     7741 ttccgaaagc agtttgtcga ggtagcgcat ctgcccgtcg agcttggctt ttttcatgta
     7801 ggggatgccg agccagacga cttggacgtg gtgcgcgcgg gcggcttcaa ggatgcggtc
     7861 ggtgcgtttc aggtattctt gcgcccattc gtcggcagcg aatttgagat agtgtttgcc
     7921 gacggggaaa tcccacgggt cgttcgggcc gaggaagacg gcgagcacgc tgatttcggg
     7981 atgtttttgc agggtttctt caatcgtttt cggccagtcg aagaatgagg ggtaggacag
     8041 ccctgtgctt tgtttgctga ggttgacgga ttcgatgccg tattgttgtt tcaggctttt
     8101 ttgcacgaag ggggcaacgc cctgcatcag cgagtcgccg gcaaaaaaga ctttgtcgcc
     8161 gctacggacg gcggcggtgt cggtgttctg tttccattct tggatttcac cggtttgagg
     8221 gacggcttcg gatgcggcgg cttcaggcgg catatctgcc gaaccgccgt cttgagccgt
     8281 cggcggcgtt tcgccggaca ggaaggcttt gatgccgtct gaaagggtgt aggcgttttc
     8341 ttgcaatgcc gcaccgctcc gccaccatcc gtaggcggca agcggttcga gcgggctgtt
     8401 gcggtggtag gtctgctgcc agtaggcgtt gatggggttt tggctgaacc acacggcaat
     8461 cagggcagac atcaatatgg aggcgaaaag ggaaagaaag tttttcatgt ccaaaccctt
     8521 aaaaattggc ataaataaag ccgggtatcc ccgagggggc caatacgata atcagcagca
     8581 ggacggcgga aacggggata aaccacagcc acatcgggat tttttccaaa cctttgaccg
     8641 cgccgtcgaa agcgcgttgc aggtaagggt agagcagcat cacggatgca aacgaggcaa
     8701 gcagcagcat atccgcctgt tgcggcgcat tccagccgtt ggcattggca aagagggcat
     8761 tgaaaactgc gcctgcatcg tcggggtttg cggtattgaa gacgacgaag gtcaggcaga
     8821 caaaatggaa ggtgatgagc cacgagagcg gcgcaaggta tttcagacgg catagtgcgt
     8881 tgcgtccgaa atagcggtcg cccgtgttga gcagcaccag cgccgtgccg tgtagtgcac
     8941 cccaaatgag gaagttccag ccgtagccgt gccagatgcc tgagagcacc attgccgcca
     9001 tcaggttgaa ctgtgtccgc aaaaagcctt ttttgctgcc gcccaagggg atgtagatgt
     9061 agtcgcgtat ccaggtggaa aggctgatgt gccatttgtc ccaaaatgcg cggatattcg
     9121 cggcacgaag cggtgcggag aagtttttgg gcagccggaa gcccagcagc attgccattc
     9181 cgataaccaa gtcggaatat ccggaaaagt ctaaaaagag ttggaaggta tagccgtaca
     9241 cgcccgccaa tacgccccag ccgtcgaatt gggcgggatt ttcaaatacg ggcgataccc
     9301 agttttccgc cagcatcccc gccagccacc attttttggc aatacccagc agaatcaggg
     9361 aaacggcgag tgcgggacgg acgggcgaac gcggttggcg ggtacggatt tgcgccaatg
     9421 cgcccgcctg ttcgccgtcc gcgcttttga atgcggcggc gcggataatc gggccggagg
     9481 taacggtggg gaaaaaactc aggtgcagca gcagttcgtg ccactcgaaa cgcgcggcgt
     9541 gcggcgcacg gaagcagtaa accaaatagg cgattgactg gaaggtgtaa tacgaaagcc
     9601 ccagcggcat caggatgtcg attgcgccgc cttgtccggc atattgtgca atcagcgggc
     9661 ggaaaaagtc gaaatatttg aaaaagccca agacggtcag cgagacggca atgccgcacc
     9721 ccagccaaaa gcggcgcgta ttttcgcgat cggaacggag cagttcgccc aaaaggtaca
     9781 cgcaggagga ataaaggacg acgattgccg caaatacggg gccgatatgg tagagccagc
     9841 ccataccggc agccaaaagc agcaggtttt ggacggacgg gtatttcgcc aagccccagt
     9901 aaatcggcag gaaggcgaga aagaacagtg cgaactcgac agacagcagc ggcataaacc
     9961 atccttggaa taagaaaata ggcgttaaaa cacggcaaag cccgttattt taagcagggg
    10021 cggatatttc ttcaatcaaa atgccgtctg aagggcggga ggggcttcag acggcatcgc
    10081 gcggtcaggt ttggatatgg cggttcaagt tcaggcacag ccacagcccg gccgccgccg
    10141 caagcatacc ggcgatgccg atacgggaaa tgccgacata ttgcgttacc caatgtccca
    10201 acagtgcgcc gctgccgata ccgacgtt
//
LOCUS       DAWGJV010000066        10159 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWGJV010000066 DAWGJV010000000
VERSION     DAWGJV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.066"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="WHQ94_001198"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="WHQ94_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ2128475.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WHQ94_001199"
     CDS             complement(1966..2568)
                     /locus_tag="WHQ94_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ2128476.1"
                     /translation="MTEKLPPDALIEAALLTQAEPLNEKTMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            2632..2752
                     /locus_tag="WHQ94_001200"
                     /pseudo
     CDS             2632..2752
                     /locus_tag="WHQ94_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2843..3784)
                     /gene="ylqF"
                     /locus_tag="WHQ94_001201"
     CDS             complement(2843..3784)
                     /gene="ylqF"
                     /locus_tag="WHQ94_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ2128477.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPADE"
     gene            complement(3865..4719)
                     /gene="rapZ"
                     /locus_tag="WHQ94_001202"
     CDS             complement(3865..4719)
                     /gene="rapZ"
                     /locus_tag="WHQ94_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ2128478.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4700..5662)
                     /locus_tag="WHQ94_001203"
     CDS             complement(4700..5662)
                     /locus_tag="WHQ94_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ2128479.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5666..6115)
                     /gene="ptsN"
                     /locus_tag="WHQ94_001204"
     CDS             complement(5666..6115)
                     /gene="ptsN"
                     /locus_tag="WHQ94_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ2128480.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6300..7190)
                     /gene="ubiA"
                     /locus_tag="WHQ94_001205"
     CDS             complement(6300..7190)
                     /gene="ubiA"
                     /locus_tag="WHQ94_001205"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ2128481.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7220..7696)
                     /locus_tag="WHQ94_001206"
     CDS             complement(7220..7696)
                     /locus_tag="WHQ94_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ2128482.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7711..8358)
                     /gene="bioD"
                     /locus_tag="WHQ94_001207"
     CDS             complement(7711..8358)
                     /gene="bioD"
                     /locus_tag="WHQ94_001207"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ2128483.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEIVLVEGAGGLMVPLTEKLLTIDYIQQQAYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQYSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8355..9656)
                     /gene="bioA"
                     /locus_tag="WHQ94_001208"
     CDS             complement(8355..9656)
                     /gene="bioA"
                     /locus_tag="WHQ94_001208"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ2128484.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2366 a         2771 c         2552 g         2470 t
ORIGIN      
        1 gcacaatcac ttgttcaagt gattaagaaa gcgtgataat acggatttat ctgagttctg
       61 tctattgttt tagactttta tttttagtta ttttttaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaatgac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgg atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tggggaaggt gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg caactttatt cagcggacgg ggcggattgc ctttgttggc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgagg gcaacaggac
      601 gatgtcgaaa cgtgcacctt tcatggcaag gtgttgcata tgctcggtcg tttcgccgct
      661 caaacgctct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcggcggcag cttgcaggct
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcaggct cgatccggta
      841 tttccgcgcc atccccatca gttcgcccat gcgctgttcc tgtgccgcca attcattggg
      901 attgatgtcg ctgcgacctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ttcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcag cctgcaacag ctcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgagg gcttcccatt cgccttgttt aatgtccaac tgattcagtt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc aattttgata aagctgcctg acggtttccg cctgctccct
     1321 gctacccgca aatgcgtcca acaattcgcg ctgggcggct tcctgattaa gcgaatgatg
     1381 ggcgttttgc ccgtggatgt cgataagctg gctaccgacg gatttgagtt gcgccaaggt
     1441 agcggcctga ttgttgataa agctgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tcttctccgt cgtttaaaag cccctgttca tacaattctg cttttaaaac
     1561 aggcaaatgg gaaatatcga acaatgccga caactgcgct tcttttgcac cgctgcggac
     1621 ttggctgtaa tcggctttgt cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgccg gtttctccgg tcaatacggt aaagccgctt tgaaaatcca gattcagatt
     1741 ttcaacaatg acaaaatcac gcaaagaaag tgttagaagc attgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttataactaa gattttttta
     1861 aattaaaaat aagttttcct taattttgct tagtacttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggcttccg gctcttcttc atccgtaggc ggcatttcca ttaaatcggg
     2041 taaaaccagt tcgcccagtt cggtcagcgg cggaagttct tccagaccgt ccaagcgcaa
     2101 atcgctgaga aacgttgccg ttgttgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt atgccctcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca attcgcgcat ggtcttttcg tttaaaggtt cggcttgagt
     2521 caaaagcgcg gcttcaatca gcgcatcagg agggagtttt tcagtcataa catccggatt
     2581 taatagttta aatatttttc agacagcctt ggataagggt tctttcagtt ttatagtgga
     2641 ttaactttaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc
     2701 aacgccgtac tggtttaaag ttaatccact atatatgttg gttttaaatt ttacaaacag
     2761 gtcgtctgaa aaatttattc tgaggtttca gacgacctgt ttgaagatag tgaaacctct
     2821 tttggcattc cactataccg ccctactcgt ccgcaggctt ctgcccttta cgctctgctt
     2881 ttctggcttc gcgtatggct ttgagttcgg cttctttctg acgggctttt ttgagccaag
     2941 tttcccattg gttcggcgtt tcgagggtga ttctgccgat tttgccttca cggaagtcgg
     3001 taaggatgtt ttcggcggct ttttggtagt tgatccgtcc gccgctgagg actgcgccgc
     3061 gttttttggc tatccattcg agccaaacgt tttcgtccca gtggctgctg gggtctttgt
     3121 cggcttggta gcgttcttgc aacataggga ggtagtggcg gcggaggtag tctaaaagtt
     3181 cgagggcgac ttcttcttcg tccaacgcgt tgcgtccgac tgcgccgccg gcggcaaggt
     3241 tgtagccgcc ttcttcgacg atgattttcg gccacagcat tccgggggtg tcgtagagcc
     3301 agaagtcatc ggcgaggaag aggcgttgtt cggctttggt gatgccgggt tcgttgccgg
     3361 ttttggcgga ttttttgcct atcatgccgt tgatgagggt ggacttgcca acgttgggga
     3421 tgccgcagat gaggacgcgc aggggtttat ctatgccttg gcggtgggga atcatggcac
     3481 gacaggcttg ggtaattttg ccgtgtgcgc ctgtttcgga ggaatcgagg gcgatggcgc
     3541 aggtgtcggg gcggctgtta tagtgttcga gccagatttt ggtgcgctcg gggtcggcaa
     3601 gatcttgttt gttgaggatt ttaagtttag gtttgccttt ggaaagttgg gcaagcaggg
     3661 ggttttcgct ggaggcgggc attcgcgcat ccagcatttc aatcaccatg tctacgcttt
     3721 tggcgcgctc ggcgatggct tttttcgctt tgttcatatg gccgggaaac cattggattg
     3781 ccatgtcttt cctttcttct caatatttga aatgccgtct gaaacagaaa atcgggtttc
     3841 agacggcatc atcgttcaac gggattagcg gctcggcagg ttttgcgcct gtctgtgccg
     3901 tatcagcaat tcataacgcc ctttcaacct tcgggcgagt ttttcgacaa catagacgga
     3961 acggtgctgc ccgccggtac agccgatggc gacggtaacg tagctcctgc tttcatcctc
     4021 caaacgcggc agccaatgcg tcagaaattt ttcgatgtcg tcaaccattt cctgcacaag
     4081 cggctgtccg tccaaataat cccaaacggg cttgtccata ccggtgtaag gcctcaactc
     4141 gggatcgtaa tacgggttgg gcagactgcg catatcgaac ataaaatccg cgttgttcgg
     4201 cacgccgtat ttgaacccga aggactccaa aatcaccagc agccctgtac gttcgacctt
     4261 cagccactgc cggactgcat ggcggagctg ttgggcattc atcttggaag tgtcgataca
     4321 ataggcgatt tctttaagcg ggaacagcca ttcccgttct ttctttaagc tttccaacag
     4381 ggtcatatcc tgattgctca gaggatgtcc tcgcctggtt tcggaaaacc ggcggaccaa
     4441 cacgctttct tccgcctcga caaacaaaac ttcaaccctg tgccccagtc cgcgcagaga
     4501 ggcaatctgt tcccgcgcct gtccgatgtc tatgccggaa cgcacatcga cgctgaccgc
     4561 caattcggtt tcgtccgcac gctcgatatg atacgacacc agcgcgggca acatttccaa
     4621 gggcaggttg tccacgcaga aataacccga atcttccatt tggcgcagtg cgacggattt
     4681 gcccgaaccg gacaacccgc taatcaggac gattttcatg attctgttcg ttttctttaa
     4741 gctgcgtctg atggcgttcc aaaaattcgc gcgtactatc cttaccgcgc aactgaagaa
     4801 tataattgcg caccgccgct tcaaccaata cggcaaggtt gcgtccgacc gcgacaggca
     4861 gcgttaccga acggacgttg acgttgagga tggattcggt ttcggtacgg atactcaaac
     4921 ggtcgagctg cttcatatac tcgtcgtccg cctcgaccaa attgataatg agttgcagga
     4981 tttttttggg gcggatggaa gtttcgccga aaatatggcg gatattgagt atccccaagc
     5041 cgcgcacttc caaaaaatcg cgcagcatag gcgaacaccg cccttccagc gtttccgggc
     5101 cgatgcggaa cagctcgacc gcatcgtcgg caatcaggct gtggccgcgc gaaatcagct
     5161 ccaacgccaa ttcgctctta cccaaacccg aatgtccggt aatcagcacg ccgatttcaa
     5221 acacatcgag aaatacgccg tgtttgacgg acgatgccgc cagggtgcgt tgcaggtaaa
     5281 tccgcaacac gtccatcaga taggggcttt cgagtttgga agtcagcagt ggaatatcgt
     5341 ttttgtggca gtagtcgcgc agtcccgggg aaaccggcaa gccgtttgcc acaatgacca
     5401 aagacataga aatgtcgaac aggtcgccga actgataacc cgtttccccc gattcgaggc
     5461 ggttcagata ttccgactcc gccaaaccga ccacttggat ttggttgggg tggatgaaat
     5521 tcaggtgtcc gaccaaagcg aggacgggct tgtccgcctc tacgccgata cggttgtccg
     5581 cacccgaatt gccggcggcc caggcgagtt gcagtttgta ttggttgtcg tcaaacagtc
     5641 ggcggacaga gatactgggc atattttatt cttcggtcag gatggcgcgg gcttcttccg
     5701 cagaagaaac cgtcatcagc gattcgcgga tgcttttttg ggaaaacttg ccggccagtt
     5761 tggacaatac ctccaaatgc tcacccgtcg cattttccgg aaccagcaag ataaaaacca
     5821 gggaaaccgg tttgccgtcc ggtgcgtcaa atccgacggg ttcgcgcgtg cggatgaacg
     5881 cgcccgtcgc ctgcttcacg ccggcatgac ggccgtgcgg gatggcaacg ccctgcccca
     5941 aaccggtcga accgagtttt tcacgggcaa aaagacattc gaaaacatca gcatgggaca
     6001 atgaggattc gcgttccaaa agcaggcccg cttcctcaaa cagccttttt ttactgccca
     6061 cctccatatc caaaacaata tgggacaaag gcaaaatttc gccgataagg ctcataagct
     6121 tctcttttca gacatcgcaa aacagaaaga ttgtaccgac tgccggggca aatctcaatc
     6181 ccgcatacgg tacgggctga cataacacag cgttttaaaa acatatttta acgcttttcg
     6241 gcacagatag aaatgccgtc caaagcagtt tacggctctt cagacggcat tgccctgcct
     6301 tatttcgcga aaaacgtatg ggcaaaaatg gcggtaaacc acacccaacc aattctgttg
     6361 tttgccaaaa acgtttcaaa acagatttgc cggacgcggc ttttgatggc ggcatattgg
     6421 cggtattgca gcagcaggac gatggggatt gccgtccaat atgcccatgc cgcaccgata
     6481 accgccccca atactgccat cagcagggta aagccgccat gacacagcat aacggcggcg
     6541 atgtcgtaac gcccgaacgt gacggcggag gttttgatgc cgattttcaa atcgtcctct
     6601 ttgtccgcca tggcgtaaac agtgtcgtaa gccagtgtcc ataaaacatt ggcggtaaaa
     6661 agtatccatg cttcaacggg gacgctgttg cccactgcgg caaacgccat cgggataccg
     6721 aaggaaaagg caagcccgag atagagttgg ggaatcggaa aaaaacgttt ggtaaacggg
     6781 taagtcagcg caagaaacag ggcgggcagg ctcatcagcc aagtcagatg attcagcgga
     6841 atcaggcaca atgcggcaag caggcacaaa aatgccgtca gcagcagggc ttcttttttc
     6901 ttgaccctgc cctgtgcgaa cggacggttt ttggtgcgct cgacagcacc gtcaaaatcg
     6961 cggtcggcaa aatcgttgat gacgcaaccg gcactgcgca ttaaaaacgt accgattgta
     7021 aacgccgcca ataccgccaa atcgggaatg ccgtctgaag ccagccacaa tgcccagtag
     7081 gtcggccaca gtaaaagcag cgtcccaatg ggcttgtccg cccgcatcag gcgcaggtac
     7141 acatccaaac gatcggacag tcgtaaaaat aaaggggatt taggattcat attgccgcac
     7201 agcttgaaaa aacggtattt tatccgataa agcgtttcag ttcgggcaga aaatactcgg
     7261 tcagcagcat ttcctcgccg tgacgggaaa accgagaacg ccgcgcggca aagtaccgtc
     7321 cgcatccttc gccggaaacg gcaaactcaa acgccgaacg cgccccttcc aaatcggctt
     7381 gaaacagacg ctcgcccaaa gggcgcgtgc cgcagtccaa aatgttttgc caaaacgccg
     7441 aacctatccg gcattcgctc cttgcctcaa caacagggat acggtccagc ttcaacaaaa
     7501 cttcgcgcac cagcctccct ccgcattccg tctccaattc gcccagtttc agcagttcca
     7561 ccgaaaatgt atgcggcaag gcgcgcaatg cggcggtcag cgaccgggct ttcaataacc
     7621 ggctcatcgg caggtcgatg ccgtctgaaa cggcggcggg caagtcgggc agccatttcc
     7681 caaataggtg ttccatattt ttcccaatct ttataccgcg tctgtttttg ccaactccat
     7741 ccattccgct tcggaaaaat cggctttcag acggcatttc aagtagctca ggctgtattg
     7801 ggcgatgtgt gcgtcgcggc tgtcatgaat atggttgaat acgaggctgt gcaagcgaat
     7861 gccgtattgt ttgagcgcgg cgaaactgag cagggtgtga ttaatgctgc cgagccgacc
     7921 gctggtaacg aggatgacgg gataagcctg ttgctgaata taatcaatgg ttaacagctt
     7981 ttccgtcagc ggaaccatca aaccgcccgc gccttcgacc aagactattt cgtattgtgc
     8041 cgccaattct tgcgtagcgg tacggatttt gtccaagtcc aaagccctgc cgtccagacg
     8101 ggcggcgaga tggggcgagg cgggatagct gaagatttca ggcatagtca gccgccgttt
     8161 gtcggcttcc tgcatgggta tgcccatgat tttgcggtga acggcgatgt cttcggcgat
     8221 gtcttggcaa ccggtttgca cgggcttttg cgtaatcacg cttttgccct gctgcaacaa
     8281 ttgttttgcc aacataccgg tggcgacagt tttgccgatg tccgtgtcta tgccgctgac
     8341 gaagtaaacg cctttcattt gctgtgttcc ttcaagattt gcacggtttt gtcggcaagt
     8401 ttggtcaaaa cgccgtctga aataatatac ggcggcatca gatataccag cctgccgaac
     8461 gggcgcaccc aaatgccctg cgccacgcaa tctgcttgaa aacgcgccat atccacgcct
     8521 ttatccagct cgatcacgcc gatggcaccc aaaacgcgta cgtctttcac gccgcgaatg
     8581 tcccacgcgg ctttcagacg gccttttaag atgctttcaa tgcggcggat atttgcctgc
     8641 cagtcttgag acaaaagcag tttgaccgaa gcgcaggcaa cggcacacgc cagcgggttt
     8701 gccataaacg tcgggccgtg cataaacacg cccgcttcgc cgcgcgaaat cgtttcggta
     8761 actttttgcg aagtgattgc tgccgccagc gtcatatagc cgccgctcaa acccttgccg
     8821 atgcacataa tatccggcac gacctccgcg tgttcgcagg caaacatctt gcccgtgcgc
     8881 ccgaatccgg tggcgatttc atcaaaaatc aaaacgatat caaactcgtc gcacaaatcg
     8941 cgcaatccgc gaagatactg cggatgataa aaatacatgc cgcccgcgcc ctgcacgacc
     9001 ggctctaaaa taaaggcggc aatatccgca tgatgcactt caaacaaggc gcggacaggc
     9061 tgcaaatccg cctcgtccca ttcatcgtcg aaacggcttg tcgggttatc gacaaaataa
     9121 cgctgcggca acgcgctgcc gaaaatatgg tgcatccccg tttccggatc gcagacggac
     9181 atcgcgttcc aagtatcgcc gtgatacccg cgccgcaccg tcgcaatatt ctgcttcgcc
     9241 gtcaaccccc gcacctgccg gtattgcacc gccattttca acgcaacttc caccgaaacc
     9301 gaacctgaat ccgcataaaa aatacggtcc aacccctgcg gcaaaatccc aaccaacaat
     9361 ttacccagct ccaccgcagg ctcatgcgtc aaaccgccga acatcacatg cgacatttgt
     9421 ttaatctgcg tctcaaccgc ctgattcaaa acaggatgat tgtaaccgtg tatcgcacac
     9481 caccaggacg acatcccgtc aaccagccgc gtgccgtccg ccaattcgat aaacacccct
     9541 tctgcacgtt tgacaggata aacgggaagc ggatcggtca tggaagtata gggatggagc
     9601 agatgggtac ggtcgaaatc aagtaatgat gatgtgtgtt gatgttcaga cggcataagt
     9661 ttctctcttt tcttattgct gcattcaaac gcaaacgcgt attctactcc gacagaccgt
     9721 ttcccacacc tctccatccg tttcgggcgc aaaaccgcga aacaaatcgt tccgcaacat
     9781 aagcgcacac cgtttcgcat tcctcaagcc cgattggaat cagacggccc aacgcccaat
     9841 accgtttcac gcagcctccg cccaacgctt caatcagtca tagacaaaac ggcaatccat
     9901 acgatacaga tcatagcaac agccatcgca acagcgttgg caaaatcagg ggactccgac
     9961 ataggcgcat agcatctgcc gatgcacggc tcctcattcg gctctatgaa taccataccc
    10021 atcacaaaat ccaccgccaa aaccaggcac ggcttcttat acttatgata gatttccacc
    10081 atcctgcccc atatatccca aacattcata ccgcatatcc cgcaggcaac aaattccgat
    10141 tgaaggttac agccctatt
//
LOCUS       DAWGJV010000067        10034 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWGJV010000067 DAWGJV010000000
VERSION     DAWGJV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.067"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(121..1338)
                     /locus_tag="WHQ94_001209"
     CDS             complement(121..1338)
                     /locus_tag="WHQ94_001209"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ2128485.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1540..2268)
                     /gene="rph"
                     /locus_tag="WHQ94_001210"
     CDS             complement(1540..2268)
                     /gene="rph"
                     /locus_tag="WHQ94_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ2128486.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2350..2814)
                     /locus_tag="WHQ94_001211"
     CDS             complement(2350..2814)
                     /locus_tag="WHQ94_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ2128487.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3031..3807
                     /locus_tag="WHQ94_001212"
     CDS             3031..3807
                     /locus_tag="WHQ94_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ2128488.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4052..4906
                     /locus_tag="WHQ94_001213"
     CDS             4052..4906
                     /locus_tag="WHQ94_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ2128489.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4947..6155
                     /gene="pncB"
                     /locus_tag="WHQ94_001214"
     CDS             4947..6155
                     /gene="pncB"
                     /locus_tag="WHQ94_001214"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ2128490.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6247..7965
                     /gene="argS"
                     /locus_tag="WHQ94_001215"
     CDS             6247..7965
                     /gene="argS"
                     /locus_tag="WHQ94_001215"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ2128491.1"
                     /translation="MNLHQTVEREAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKIADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8515..9933
                     /locus_tag="WHQ94_001216"
     CDS             8515..9933
                     /locus_tag="WHQ94_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ2128492.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKITGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAIVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITVGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2315 a         2959 c         2527 g         2233 t
ORIGIN      
        1 atttaatcca ctatattttt tgttccaaag tcaaaatatg ccgtccgaac attcgggcgg
       61 cagacaaaac ggcactgccc gataaaggca gtgccgttgt ccgtttcaaa ccgtgaaaca
      121 tcagcccaaa ttaaaggctt tatgcaatac cctggttgcc agttccatgt atttttcatc
      181 aatcaatacg gaaactttga tttcggaggt ggaaatcatt tggatgttga taccctcttc
      241 ggcgagcgtg cggaagattt tggcggctac accgacgtgc gaacgcatac ccaaaccgac
      301 tgcggagact ttgcatacgg tgtcgtcgcc atcaatagaa gccgcgccga tactgtcttg
      361 gcgttccgac aggatttcca aagtctgctt gtaatcgccg cgcggtacgg taaaggaaaa
      421 atcggttgtg ccttcgctgc cgacattttg gataatcata tcgacttcaa tgttggcatc
      481 ggcaaccgcg cccaaaatct gataggcaac gccgggtttg tcgggtacgc cgcgcacgtt
      541 gatgcgggct tggtttttat cgaatgcgat accggttacg gcagctcttt ccatgttgtc
      601 gtcctcttca aaggtaatta aggtgccatt gccgccgtct tgcaggctgc tcagtacgcg
      661 caggcgcact ttgtactttc cggcgaattc tactgaacgg atttgcaaga ctttcgagcc
      721 gaggcttgcc aattcgatca tttcttcaaa tgtaaccgta tccatgcggc gcgcttcggg
      781 tacgacgcgc ggatcggtgg tgtacacgcc gtccacgtcg gtatagattt ggcattcgtc
      841 cgctttgagg gctgcggcaa gcgcaacggc ggaagtgtcg gaaccgccgc gtccgagcgt
      901 ggaaatatcg ccttcactgc tgatgccttg gaagccggca acgatgacga ctttgccggc
      961 ggtaaggtcg gcacgcattt tttcgtcatc aatgctttcg atgcgggctt tggtgtgggc
     1021 ggtatcggtt ttgagggcga cctgccagcc tgtgtagctt ttggcatcca cgccgatgtc
     1081 tttcaatgcc atcgccaaaa ggccgatggt tacttgttcg ccggtggaca aaaccacgtc
     1141 caattcgcgc ggatcgggat gctcctgcat ttcgtgcgcc aacgcgacca ggcggttggt
     1201 ttcaccgctc atggcggata cgacgacgac gatgtcgtgt ccttcggcgc gggctttggc
     1261 gacacgtttg gccacgtttt taatgcgttc gggcgagcct acggatgtgc cgccgtattt
     1321 gtgtacgatt aacgccatgt ttcgtgcttt cttgtgtggg attggtcggg cagcatggtt
     1381 tgctggaaaa gcggctatta ttactatttt ttacatgaag ttcaagaacg gactgcgctt
     1441 tcccgcctgc cgtttgacag cggtcagcga aaaacctgtt ctttcagatt gttgacaaaa
     1501 tgccgtctga acggttttca gacggcatcc ggacgacaat caggcggcgg acaacgcatt
     1561 ttgctggtgt tgcagcagtt cgcctatgcc tttttgcgcc agtgcaacca gtttgcccaa
     1621 ttcgtccaaa ctgaacggcg cgtcttccgc cgtaccttgg atttcgatga tttttcccga
     1681 tgcggtcatg acgatattca catcactgtc gcaaccggag tcttcgggat aatccaaatc
     1741 caaaagcggc acgccgttca ctacgcctac tgacacagcg gcaacggctt cgcggatggg
     1801 gttttcactc aaaatgccgt ctgaaaccag tttgccgacg gcgatttgca gcgcgacaaa
     1861 cgcaccggta atcgaagccg tacgcgtacc gccgtctgcc tgaatcacgt cgcagtcaat
     1921 caagatttgg cgttcgccga gtttttccat atccacgacc gcgcgcagcg aacgcccgat
     1981 aaggcgttgg atttcttgtg tgcgcccgga ctgtttgccc gccgaagctt cgcggcgcat
     2041 ccgggaagca gttgaggcag gcagcatccc gtattccgcc gttacccagc cttggttttt
     2101 accgcgcaga aacggcggga cgttttcatc tatggaagcg gtacaaatca ctttggtatt
     2161 gccgcattca ataaggcacg aaccgtccgt atgcggcagg aaatgagggg tgattttgat
     2221 atcgcgcagg ctgtcggcgg cgcgcgagat gcggatgtaa tcaggcatac tgccctcccg
     2281 ttaaaaacag ataaattaaa aagccttaaa tatgaaaaat cacatttaag gccttcaaac
     2341 tgaaaatttc tacgcctctt cggctttgct gcggataatc aaaagcggca ggtggctttg
     2401 gcgcattacc gtttcggcaa aactacccat taaaaggtgc atcagtccgg tacgtccgtg
     2461 cgtacccaac accagcaggt cggcaccgtt ttcatcggca taatcaacca aatcctgcgc
     2521 catttcacgc gcacccttat tggcaaccag caggtgtttg acggtatttt ccacacccag
     2581 ttcccgggcg gtgcgctcgg cggcatccaa aacttcgttg ccttgcgcga cggcggcggc
     2641 ttcgtagctt tcgtgttgca aaaattcggg ggcgagcgcc atatattcgg caggattggc
     2701 aacgtgtacc aaagtcagac gcgcaccgtt gaccccggca agctcggcgg catgtttcag
     2761 ggcattgatg gacgtttcac tgccgtcaac ggcaacaacc aaatgtttgt acatatcgta
     2821 ttctcctttt gcaccgcctc gcggtgccct cttgtcggat gggcgcaggg acagtttgcg
     2881 ctgtttcatt atagacccgc cgtcgggctt tatacaacag ccgaacagcc cgaccgcttt
     2941 ccagtataat atgccgcttc cgtgcagtca ggcatttttt gccggctttc gttcactttt
     3001 tgatttgacg caatcttgca ggattcgacc atgtccgaca acgctttgac ctcttcgcga
     3061 cgcttcggcg gcatcgccag actctacgga gactctgcct tggcgcactt ttcacaggca
     3121 cacgtctgcg tagtcggcgt gggcggtgtc ggctcgtggg cggtcgaggc tttggcgcgg
     3181 acgggcatcg gacgtttgac tttgattgat ttggacaacg ttgccgaatc gaatgtcaac
     3241 cgccagctgc acgccctgac cggagacttc ggcaaagcaa aagttaccgc cttgcgcgaa
     3301 cgcgtcgccc aaatcaaccc agaatgccaa gtgtttgaaa ttgaagattt cgttaccgaa
     3361 gacaatttgc cggaatactt cggaaaaggt tttgatttcg tcatcgacgc gatcgaccaa
     3421 gtgcgcgtca aagcagcaat ggcggcttat tttgtggaac gcaaacaacc gtttgtcctc
     3481 agcggcggcg cgggcggaca aaaaaatccg gcgttaatcc aaaccgccga tttgagccgc
     3541 gtaacccacg acccgctgct tgccaacctg cgctacacct tgcggaaacg ctacggattc
     3601 agccgcgata cgaaagcaaa tatgcgcgtg ccttgcgtgt attcgaccga aaatatcgtg
     3661 ccgccgcagt ctagggaggc ttgttcggca gatgccgctc cgcaaggctt gtcgtgcgcc
     3721 ggctacggtg caagcatgct cgttaccgct tcgttcgggc tatattgcgc acaggcggcg
     3781 gtggaacaca tcgcagacaa aaaataagca atgccgtctg aaacaggatt cagacggcat
     3841 ttgaacaaac gcggcaatca agctgtcggt tcagaaaaac caacatcggc acgcctaaaa
     3901 ccgtattcac aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt
     3961 ctgaacattt gcccgaccac attatacgtg aatgtcggca gattgttttc ttttgtaaac
     4021 ttatattaaa atccacttac cgattcacgc catgccgccc atccctgccc catccgcacc
     4081 atccgagcac actgtcgcat gggtattcgg ccaacccgtt accgatttgc cgcaggattt
     4141 gtttatccca cccgacgcgc tgaaagtcgt attgggcagt tttcaaggcc ctttggatct
     4201 actgctgtat ctgatccgca agcagaacat cgatgttctc gatattccga tggtgaagat
     4261 taccgagcag tatctgcact acatcgccca aatagaagcc tatcagtttg atttggcggc
     4321 ggaatacctg cttatggcgg ccatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg
     4381 taccgaaacc gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgct
     4441 ggcttacgaa cagatgaagc tggcggcgca gggtttggac gcgctgcccc gagccggacg
     4501 ggatttcgcg tgggcttacc tgccgctgga aattgccgtc gaagccaagc tgcccgaagt
     4561 ctatattacc gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacacacgcg
     4621 cagccacgaa gtaatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg
     4681 ccgtttgaac ggacacggga tatgcaggtt tcacgacctg ttcaatcccg aacagggcgc
     4741 ggcttacgtg gtcgtcaact tcatcgcact gttggagctt gccaaagaag gtttggtcgg
     4801 aatcgtacag gaagtcggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcaca
     4861 ttcagacggc atttccggca cacggggcgg gcgcgatgtg ttctaatacg ccccaagccg
     4921 ccaccaaaaa tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg
     4981 acctctacaa attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc
     5041 tttacgaatt ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcagggaag
     5101 acttggaagc cgaactcgac gcgctctgcc aactacgctt cacccacgac gaactcgact
     5161 acctccgtag cctgcgtttc attaaaagcg actttgtcga ttatctcgaa ctcttccagc
     5221 tccaacgccg ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgcatcg
     5281 aaggtccgat gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac
     5341 tttacttccg ccgcctggaa acccctgcag tcatagaaga aggcgaacgc cggcttcaag
     5401 ccaaagccgc gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgaa ccgcccttcc
     5461 tgatttccga cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc
     5521 gcaccctgct cgaagccgcc ccaagcatcg tgcgcggcac cagcaatgtc tttctcgcca
     5581 aaaaactcgg catcaccccc atcggcacca tggcgcacga gttcctccaa gccttccagg
     5641 ccctcgacgt acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat
     5701 accggggcga tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg
     5761 atttcgacct ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt
     5821 acgtttgggg cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca
     5881 aaatgctgac cttctccgac gggctggaca tcgaacgctc ttgggcatta caccaatatt
     5941 tcaaagaccg cttcaaaacc ggcttcggca tcggcaccaa cctgaccaac gatatggggc
     6001 atacgccttt gaatatcgtc ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc
     6061 tgtccgactc tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc
     6121 aagtgttcga cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc
     6181 tgtttctgcc gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg
     6241 aacttcatga acctacatca aaccgtcgaa cgcgaagccg ccgccgcctt tgccgccgca
     6301 ggcatcgccg gcagccccgt tgtcttgcag ccgaccaaaa atgccgaaca cggcgatttc
     6361 caaatcaacg gcgtgatggg tgcggcgaaa aaagccaaac aaaacccgcg cgaactggcg
     6421 caaaagatcg ccgacgcatt ggcaggcaac gccgtcatcg aaagcgcgga agtcgccggt
     6481 ccgggcttta tcaacctgcg cctgcgcccc gaatttctcg cgcaaaacat tcagacggcc
     6541 ttgaacgacg cgcgtttcgg cgtggcaaaa accgacaaac cgcaaaccgt cgttatcgac
     6601 tattcctcgc ccaatctggc aaaagaaatg cacgtcggcc atctgcgttc cagcatcatc
     6661 ggcgacagca tttcgcgcgt gtcagaattt atgggcaata ccgttatccg tcaaaaccac
     6721 gtcggcgact ggggtacgca gtttggcatg ttggtcgctt atttggtcga gcagcaaaaa
     6781 gacaatgccg cattcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc
     6841 tttgacgaag accccgcctt tgccgacacc gcgcgcgaat acgttgtgaa gctgcaaggc
     6901 ggcgatgaaa ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc
     6961 caagccgttt acgacacgct gggcttgaag ctgcgccccg aagacgtggc gggcgaatcg
     7021 aaatacaacg acgatttgca gcccgtagtc gatgatttgg ttcaaaaagg tctggcagtc
     7081 gaagacgacg gcgcaaaagt cgtgttcttg gacgagttta aaaacaaaga gggcgaacct
     7141 gccgcattta tcgtgcaaaa acaaggcggc ggcttccttt atgcttccac cgacttggcg
     7201 tgtttacgct accgtgtcgg cacgcttcat gccgaccgcc tgctgtacgt cgtcgaccac
     7261 cgccaagccc tgcacttcga acaacttttc accacttcac gcaaagccgg ctatctgccg
     7321 gaaaacgtcg gcgcggcatt tgtcggcttc ggcacgatga tgggcaaaga cggcaaaccg
     7381 ttcaaaacac gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgaa
     7441 cgcgccaccg ctttggtgaa agaaaaaaat cccgaattgg gtgcggacga agccgctaaa
     7501 atcggcaaaa ccgtcggcat cggctcaatc aaatacgccg atttgagcaa aaaccgcacc
     7561 agcgattatg tgttcgactg ggacgccatg ctctcgtttg aaggcaacac cgccccctat
     7621 ctgcaatacg cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggatgca
     7681 aatgcgccaa ccgttttgac cgaaccgctg gaaaaacagc ttgccgccga gctgctgaaa
     7741 tttgaagacg tactgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac
     7801 ctctatcaaa ttgcgaccct gttcagccgc ttctacgaag cctgtccgat actcaaagcc
     7861 gaaggcgcaa gccgcaacag ccgcctgcaa ctggcaaaac tcaccggcga cacgctgaaa
     7921 caaggcttgg atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga
     7981 ttgcggacaa cagcctcgcc atccttatcc gaatctgaaa aaagcggcgc gatacaccgt
     8041 atccgccgcc cttcccaaaa tgcgaaacaa acaaacgcca agcaagcaag caagcaagca
     8101 agcaagcaag caagcaagca agcaaaaaat tataaccccc ttcctgccga cgcacgcact
     8161 ttccgcgcgg cgcattcccc ttttcccgcc cctcaaatcc gccttttctt caggcagggt
     8221 ttcagcccgc ctcttttccc tgttttcctt tccccgacac gcgtgcgcgc cccctgccgc
     8281 actgtgctgc actttcgcgc ccggacggca tcgttccgcc atccggttct ctgttttaaa
     8341 tacccctgtt tcagaaagaa atgcagatgt ttcaacacac aggacgacac ataaagcacc
     8401 gccctatgtg ttgccctgat ttggaagggg ttacgcctcc caaataaagt ctgatcctgc
     8461 cgccccgaag gacagatgtc cgagtggcga agtttcaacc gaaaaggaaa tacgatgaat
     8521 attcacaccc tgctctccaa acaatggacg ctgccgccat ttctgccgaa acggctgctg
     8581 ctgtccctgc tgatactgct ggctcccaat gcgatgtttt gggttttggc actgctgacc
     8641 gccaccgccc gcccgattgt caatttggac taccttcccg ccgcactgct gatcgccctg
     8701 ccttggcgtt tcgtcaaaat taccggcgta ttggcgtttt ggctggcggt tttgtttgac
     8761 gggctgatga tggtgatcca gcttttccct tttatggacc tcatcggcgc catcaacctc
     8821 gtccccttca tcctgaccgc ccccgcccct tatcagataa tgaccgggct gttgctgctg
     8881 tatatgctgg cgatgccgtt tgtgttgcag aaagccgccg ccaaaaccga cttccggcac
     8941 attgccgtct gcgccgccat tgtggcggca gccggctatt tcaccggcca tttgagttac
     9001 tacgaccggg ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt
     9061 caggcgatgc tctacaccgt tagccagaat gccgacttta ttaccgtcgg cctggtcgat
     9121 cccgtcttcc tccccttggg caatcagcag cgtgccgcca cgcatctgaa cgagccgaaa
     9181 tctcaaaaaa tcctctttat cgtcgccgaa tcttgggggc tgccggccaa tcccgaactt
     9241 caaaacgcca cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc
     9301 ggcagttttc ccttcatcgg cgcgacggtc gaaggcgaaa tgcgcgaatt gtgcgcctac
     9361 ggcggtttgc gcgggttcgc actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc
     9421 cccaaccgtt tgaaacaaga aggttatgcc acctttgcga tgcacggcgc gggcagttcg
     9481 ctttacgacc gcttcagctg gtatccgagg gcgggctttc aagaaatcaa aaccgccgaa
     9541 aacctgatcg gtaaaaaaac ctgtgccatt ttcggcggcg tgtgcgacag cgagctgttc
     9601 ggcgaagtgt cggcattttt caaaaaacac gacaagggac tgttttactg gatgacgctg
     9661 accagccacg ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa
     9721 tacggcctac ccgccgaaac cgacctctgc cgcaacttca gcctgcacac ccaattcttc
     9781 gaccaactgg cggatttaat ccaacgcccc gaaatgaaag gcgtggaagt catcatcgtc
     9841 ggcgaccatc cgccgcccgt cggcaacctc aatgaaacct tccgctacct caaacagggg
     9901 cacgtcgcct ggctgaactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca
     9961 gccttcagac ggcattttgc agacagaccg acccttcaag cccacttttt tcatcatctc
    10021 cgataaattg cttt
//
LOCUS       DAWGJV010000068         9798 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWGJV010000068 DAWGJV010000000
VERSION     DAWGJV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.068"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(13..1308)
                     /locus_tag="WHQ94_001217"
     CDS             complement(13..1308)
                     /locus_tag="WHQ94_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ2128493.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WHQ94_001218"
     CDS             complement(1434..2321)
                     /locus_tag="WHQ94_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ2128494.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WHQ94_001219"
     CDS             complement(2321..3286)
                     /locus_tag="WHQ94_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ2128495.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WHQ94_001220"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WHQ94_001220"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128496.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4622..4729)
                     /locus_tag="WHQ94_001221"
                     /pseudo
     CDS             complement(4622..4729)
                     /locus_tag="WHQ94_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WHQ94_001222"
     CDS             complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WHQ94_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ2128497.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WHQ94_001223"
     CDS             complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WHQ94_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ2128498.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WHQ94_001224"
     CDS             complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WHQ94_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ2128499.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WHQ94_001225"
     CDS             complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WHQ94_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ2128500.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8698..>9798
                     /locus_tag="WHQ94_001226"
     CDS             8698..>9798
                     /locus_tag="WHQ94_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ2128501.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2510 a         2585 c         2431 g         2272 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gtgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatacacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgtacatacc agtaaggtgc aggaacttgg cttttgacga
     3421 cgcgcccgtt ggcgagtttg atatggtata tggcaaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac caccagaaag ctggtgaggt ttgcgtttgg
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccaaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcggtc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata attttcccct gattggggct ttccataccc gccagcatac
     4261 gcagcagcgt ggattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaagcctt
     4381 ggatttgcag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaaa cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agtcgcaacg tctgatgccg tctgaaactc ttgtgaagcg cacgggcagc
     4561 atgaaatgga acaagattcc aaagaacttt atattatatt agtttatgcg gttttcgggc
     4621 aatatagtgg attaaattta aaccagtaca gcgttgcctt gccttgccgt actatttgta
     4681 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa atatggattt
     4741 gagcttgtcg gaaagcaaca gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa
     4801 agcgtaatta tttacgcaga cccaagcggg taatcaacgc gcgatacgta tcgggctggg
     4861 tacggcgcaa gtaggccagc aggcggcggc gttggctgac cattttcaac aggccgcgac
     4921 ggctgtggtg gtctttgggg ttggctttga agtggggggt caggtcgttg atgcggaaag
     4981 tcaacagagc gacttgtact tcggaagagc cggtgtcgcc ttctttgcgt tggaaatctt
     5041 taacgatttg tgctttttgt tctacggtca gtgccataat gaaaactcca aaaatataag
     5101 aatccccata gggattcaga caagtttgcc aagcctgaag acaacggcaa actccctatg
     5161 cccaagatag gacaacgtgg cattatgaca caattcccgc atttctgcac aatattttaa
     5221 gaccgtggaa gtaaccgtta aaaatgccgt ctgaagcatt gtctgcttga gacggcaatg
     5281 ttcagacggc atatgcgctt caaaccacga tttcttcttt cttcttcggt tctttaccga
     5341 tattgtcgcg gctcaaaccg aacattagca gaagcgggct ggcaaccaat acggaagaat
     5401 aaatgccgaa cacgatgcca atggtcaacg ccatagaaaa gccgtgcaag gccgcaccgc
     5461 cgaacaccag catagaaaca accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
     5521 tcatcgttgc ggtaatcgcg ttgtcgatga cttccggcac ggcatgtccg cgcatcgccg
     5581 gcttgcggaa gttttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
     5641 ccaatacggc aaggataccc gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
     5701 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
     5761 gccattcaaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
     5821 tcaatccatt acttaccaat tcctcaccga cttgcgggcc gataaattcg acttggcgca
     5881 aggtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
     5941 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
     6001 gaacctgtac atcacctatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
     6061 caccctgctg atattggact tccattaccg taccgccggt aaattcgaca gagaaattca
     6121 ggcctttggt caccaagaag aacacggcag cgataaacgt aaccaacgaa atgaaggtcg
     6181 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
     6241 actccttacc tgccgcagtt tcggcttcag gtttccatac cgcaccgatg gaaatattct
     6301 gcaatttgcg tctgcgtccg taccacagat tgaccaacgc acggaatacg acgacggacg
     6361 aatacatcga agtcagaata cccaaacagt gtacgaccgc aaaaccgcgt accgggccgg
     6421 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttggaa tcgacaatgg
     6481 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccgacacgca
     6541 attcttcgcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
     6601 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catagcagac aaaataccga
     6661 ttaggaacag tatgttggca ctcaatgcaa tggtagaaaa gaaacccatc agacgatagt
     6721 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
     6781 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggcg
     6841 cggcaagaga accggcacgc aacagcaaag acgtatcatt ggcttcggct gtcgtcatgc
     6901 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggc gcggttacaa
     6961 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
     7021 tcagttcgcc gaaaatgctg ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc
     7081 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gcccgtcagc tcgacctgtt
     7141 tgctgattag cagagtttcg ggacgatctc cgccgcttga aagcagctca taaccgctcg
     7201 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atacgcaatt
     7261 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcctga acgcccggaa
     7321 gctgcacgac gatacggtct gcacctgact gctggatgac gggttcggcc acgcccaact
     7381 cgttcacacg gttgtgcagg gtagtgatgt tctgtttgac cgcatcggaa cgcactttat
     7441 tgaccgcctc ttccgaaagc gtcaagacga tattgctgcc gtctgaattc agcgttgctt
     7501 caggaaacag cttgcgcaac tgcggcagtg ccttttgcac atcacctgca tcctgcaaag
     7561 ggacggtcag gctgtttcca gcctgacgca ccgtgccgct gcggattttt tcgcggcgca
     7621 gttcgcggcg gatgtcgccc gaataacgtt caaacgtttt ctgcatcgcc gctttcatat
     7681 cgacctgcat ggtgaaatgc acgccgccgc gcaggtccaa acccaaaaac atcggattgg
     7741 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
     7801 cttcgcccaa agtgttttcg atgacgtcgc gcgctttaag ctgcgtttct gtgtctttga
     7861 aacgcacttt cagtgaattg tccacaacaa acatcccgtc ggtctgaata cccgcgtttt
     7921 gcagcgcggc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
     7981 tcgatacctg cacggcgggt gtttcgccga ataggttggg cagcgaatac actgcggcaa
     8041 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
     8101 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
     8161 aaaaaccctt cccagccagg caagaccgga aggcggcatc ttgaatatgc cgccctgcgt
     8221 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
     8281 cagatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttctact
     8341 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
     8401 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccgacttt caaggcggca
     8461 agcattgcct gatgcgcttt gaatttcttt tgctgcggac gcatgatcag gaagtagaac
     8521 accaccataa tcaacactaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
     8581 ttctttctaa tatggttgaa aatcgagagg ggtatataat aacataagac cgtaaacaat
     8641 atatcgggtt tgccgtccgt accgaccgta taccgcagcc tgcccgtcca caaacccatg
     8701 tccttgaccc gcctaatctt gaaattctat gcactgttgc gccttttttt gggcaaaaac
     8761 gcccgcaccg catggatttc gcatcccgcc tgtgccgggc acgaacccgg cgcaaaccat
     8821 cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg
     8881 cagcacctcc aaaccataga ggcggaagaa atcagcgata cgcgcctcgc acttgtccac
     8941 tcgagcaaat atctgaaccg tttggaatct tgcctgcccc aaaaaggcaa gattttccgc
     9001 ctggataacg acactgcaat cagcacaggc tcgctgtctg ccgcacactt tgccgccggt
     9061 tcggcagttc aggcagtcga catggtcatg aaccgtaaag catggcatgc cttttgcgcc
     9121 gcccgcccgc ccggacacca tgcgggcagc ggcaaagccg gcggattctg cctgctgaac
     9181 aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc
     9241 atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc
     9301 atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacgatatg
     9361 cccgacggcg gcaatatggt gcacctgccc ttgccgccgg gaacgggcag ccgcacattc
     9421 cgcgaagccg tccgcaggca gtggctgccc cgacttgccg cattcaaacc cgaactggtg
     9481 ctgctgtcgg caggattcga cgcacaccgt ctagacgaat cgggcaggct caacctgcac
     9541 gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatcggg ctgccccggc
     9601 aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc
     9661 gagcatatcc gagtgctggc agggctgggc aaatccgatg ccgcaaccgc ctatcagaaa
     9721 acactggacc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct
     9781 acccaatccg accgttac
//
LOCUS       DAWGJV010000069         9675 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWGJV010000069 DAWGJV010000000
VERSION     DAWGJV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.069"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(38..763)
                     /locus_tag="WHQ94_001227"
     CDS             complement(38..763)
                     /locus_tag="WHQ94_001227"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ2128502.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYQN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPCSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="WHQ94_001228"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="WHQ94_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ2128503.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="WHQ94_001229"
     CDS             1486..1863
                     /locus_tag="WHQ94_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ2128504.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="WHQ94_001230"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="WHQ94_001230"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ2128505.1"
                     /translation="MTPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2796..3206)
                     /locus_tag="WHQ94_001231"
     CDS             complement(2796..3206)
                     /locus_tag="WHQ94_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128506.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(3208..3454)
                     /locus_tag="WHQ94_001232"
                     /pseudo
     CDS             complement(3208..3454)
                     /locus_tag="WHQ94_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3462..4616)
                     /gene="lpxB"
                     /locus_tag="WHQ94_001233"
     CDS             complement(3462..4616)
                     /gene="lpxB"
                     /locus_tag="WHQ94_001233"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ2128507.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARFTGIGGE
                     LMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRTGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4682..5674)
                     /locus_tag="WHQ94_001234"
     CDS             complement(4682..5674)
                     /locus_tag="WHQ94_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ2128508.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6167..8926
                     /locus_tag="WHQ94_001235"
     CDS             6167..8926
                     /locus_tag="WHQ94_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ2128509.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEMQTGKRRRNGSRSE
                     RSQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2428 a         3077 c         2274 g         1896 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aaagcgattt
      241 tcgacgcatc cggttgtaat gcgaacatcg attcgccgta aaacaccctg ccgatttgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgtagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gactgcaacc cgatagctgc cgttgcgtag cgttttcgcc agcgagcacg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg caaaaaccaa ggaaacacgc cgttctgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg tgccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcatg gtggtcgcct ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatgat ggcgttgcag aatacccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gtgccgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaaccgcg cgccgttgcc
     1621 gccctccgcc ccggcatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagaac gcagcaatct gaccgtctat tttgaaaacg gcgtactcgt ccgcaccgaa
     1801 ggcgacgtcc tgcaaaacgc tgccgaagcc ctcaaagacc gccaaaacac agacaaacca
     1861 taaggaacac acatgacacc gctcaaaatc gctatcgccg gcgtcaacgg ccgcatgggg
     1921 cgcgtattgg ttgaagccgt caacaaccat cccgacaccg tcctttccgg cgcgctcgaa
     1981 cactcgggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacgccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agcccaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ctatccacac tgccgccgaa
     2221 caaacaggca tcgttttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacatc
     2281 ctcgacaccg tcgcacgcgt attaaacgaa ggctacgaca tcgaaatcat tgaaggccac
     2341 caccgccaca aagtcgatgc ccccagcggc accgcgttac gcatgggcga agtcatcgcc
     2401 ggcgcgctcg gcagagacct caaacaatgc gccgtttacg gccgcgaagg ccacaccggt
     2461 ccgcgcgatc cgtcgaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgag cgcgtggaaa tcacccacaa ggccagcagc
     2581 cgcatgacct ttgccgccgg tgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggacgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccaacaggc tccgtatcgg catatcaata
     2761 aagttgcttg cgcggcatga caagctacgg ctcgtttaat tgaaaccctc ctgatctaaa
     2821 aattctaact ctatttcccg gcaaactata tctatcctaa aagcagtcgg aaaatcatac
     2881 ctaccgccta tcaattccag cgcacctcca taatccaaga tttcatcatc ctgctttaat
     2941 tcgccaagtt ccaatgtatt atcgatattt atatcttggc tctttgtagc gtatccatag
     3001 acaaatttca gatagctgaa ctgcttgaat ttaattttta cccagtctgt ataatttggt
     3061 gtaggaatct cttgaggaaa gtccggataa tccacattct ggacgatgat atggaattca
     3121 tcgtttttca aagtatagtt taatagagtg ccctcatata tatacagaaa ttgataatct
     3181 ttttttggaa tggtataagt tgtcatatta ttttaccttt tcaaaaacaa tgagtttaag
     3241 attttttgag tccaagttgg ttctactact cttatgaaat ttcagttgtt taaactcttc
     3301 ctttaacggc ccattatatt ttaaccttaa tcccactcga gccagttctc cttcatttgg
     3361 aattcctctt aaatacttat tgtttgatgt gccattaatc acaatttctc cccccttttt
     3421 ttgtaatacg tgctgcttca agaagataat ccattaaccg ctcaacatcc cgcctcttcc
     3481 aaaaccgcgc gcgcggccaa atccgccgta tcttttttca acagcaggtg caacgccctg
     3541 aaatcctgtt gcagcgcggc aaccttatcg gggtgttcgt accagtccgc caacgccgcc
     3601 gccagttttt ccggtacggc atcatgttga agcagctccg gcacagcctc cttacccaac
     3661 aggatattcg gcaggccgac atgcggcact ttgattttgc gtttcacata agcataagtc
     3721 agcggcgaaa tcttgtagct gatgaccatc ggacgcttgc acagagccac ttccaaagtt
     3781 gccgtaccgc tcgttaccag caccgcatcc gccgccctgc acactgtttc agactgtctg
     3841 tcgattaccg tcagcggcaa tccggcaaac tccggccgct gcaaaacttc cgccaaacgc
     3901 cgcttcgtcg ccgccgttgc ggcaggcagc aggaagcgtg cggcaggata gcgttccaac
     3961 aacaataatg ccgtctgaaa aaacaccggc gccatatagt cgatttcgct gacgcggctg
     4021 ccgggcagca gggcgaatac ggggatgccg gcatccacgc ccaaagtttt ccgcgccgtt
     4081 tcacggtcgt cttccaaggg cataagctgc gccatcggat gaccgacaaa ctccgcacgt
     4141 ccgcccgcat cgagataaag ctgcggctcc atcgggaaca ggcacagcac gcggttgacc
     4201 tgatgcacga ttttgcccac gcgttcccgc cgccacgccc acaccgacgg actgacataa
     4261 tgcacggtcg gaatgcccgt ccgtttcagc ttttccgcca cacccaaatt gaaatcgggc
     4321 gcatcgatac cgacaaagac atcaggtttc aacgacagca aatcccgtac cagccccctg
     4381 cgtatccgta aaatttccgg cagccgcctg accacttcga caaagccgcg caccgccagc
     4441 cgctcctgat cataaaggct ctcgaaacct tccgccttca tcagttcccc gccgataccg
     4501 gtaaaccgcg cctgcgggca acgctcgcgg atggcgcgta tcaggtgcgc ccccaacagg
     4561 tcgcccgacg cttcgccgac actgacggca atcaaagggc tttttttatc aaccatattc
     4621 gttcttcttc acatactttc acgtcccgat gccgtctgaa ggcttcagac ggcatcgggg
     4681 ttcacatttt cgtcccgttt tccaacatca ccgcaaactg tgccaaatcc ggcggcagcc
     4741 ccacctttaa caccagcggc tcgcccgtga gcggatggtt caggtgcagc tcggacgcgt
     4801 gcaaaaacat ccgcttcaaa cccaacttct gcaaacgacg gttcgcctga taatcgccgt
     4861 agcgttcgtc gcccgcaatc ggacagcctt gagattgcag gtggacgcgg atttggtgcg
     4921 tgcgccccgt tttcaacgtc gcccgcacca aagtcaggtg cgacagcccg acaccgtgca
     4981 aaatgccgtc tgaaaaacgg cttaacacac ggaacaccgt atgcgccgac tgcccgtccg
     5041 cactgacgcg caccatcttt tcgccttgtg cgccggtata tttgaacagg ggcagtttga
     5101 catggaaatt gtcgtccggc agtttgccca cccccagcgc aaggtagatt tttttggggt
     5161 ggtcgttacg gatggcttcg tgaagtttga cgagcgcgct gcgtttcttc gccaccatca
     5221 acaagccgct cgtatccttg tccaaacgat gaaccaactc caaatacttc gcctccggac
     5281 gggcgcggcg caactgttcg ataacgccga aactcacgcc gctgccgccg tggacggcaa
     5341 cgccggacgg tttgtcgatg accaaaagcg catcgtcttc gtaaacaacg tcaaacgcac
     5401 gcgccggtac ggcggcacgc ctttcagacg gcatttcctt ctccgccacg cgcacaggcg
     5461 gaatccgcac cgtatccccc tccgcaatac ggctgtcggg tttgcagcgt ttcttgttca
     5521 accgcacctc gccggcgcgg ataatgcggt ggatatggct cttgggaaca cccttgagga
     5581 tttttatcag atagttatca aggcgttgac ccgcctcatg ttcggcaacc cctatcaagc
     5641 tgaccgaatc tttgcttatt tcgtgcgttt tcatctataa tccgaacatc cgtttcagca
     5701 aaaagcgcgc ccgccgcgcc tcctgaaacg gtttacttta aaacggaatt ttatattaaa
     5761 aacgcactcc gcaaagcatt ttccctgcgc ctgttcccag ccggcaggcg cagaacgtaa
     5821 tcaagtttga attgattttg ccgtttcggc gcggaagtaa gacggcagcc gggcccaagt
     5881 cccaaacgca aaccgcccaa aacacaggca tcacgataac aagaagaagc cggcccccat
     5941 ttttccgatc gcagcgttcg gaacgcggca gacaaaccgc cgcatcgatc tttatagcct
     6001 tctgtatccg acccttccgc acacggttct ccggaacgtg catccctgcg gattttcaac
     6061 tcaaaaactg attaccggtt ttatccgaaa acatcggaaa acccagcctt acggaatgcc
     6121 gaaccgggca tgtgccgtct gaacgccgcc tgcccacgag gtgatcatga aaagaatgtt
     6181 atttaacgca acgcaggccg aagagctgcg cgttgccatc gtcgacggac aaaacctgct
     6241 ggatttggac atcgaaacgc tgggcaaaga acagcgcaaa ggcaatatct acaaaggcat
     6301 cattacccgc atcgagccgt cgctggaagc gtgtttcgtg gattacggaa ccgaccgcca
     6361 cggcttcttg ccgtttaaag aagtctcccg ctcatacttc caagactacg aaggcggacg
     6421 cgcgcgcatc caagacgtgc tcaaagaagg catggaagtc atcgtccaag tcgaaaaaga
     6481 cgagcgcggc aacaaaggcg ccgcgctgac caccttcatc agcctcgccg gacgctatct
     6541 ggtattgatg ccgaacaacc cgcgcggcgg cggcgtatcc cgccgtatcg aaggtgaaga
     6601 gcgtcaagag ttgaaagccg ccatggcgga actcgacatt ccgaacggca tgagcatcat
     6661 cgcccgtacc gccggcatcg gccgcagcgc ggaagagttg gaatgggact tgaactacct
     6721 caaacaactc tggcaggcga ttgaagaagc aggaaaagcg catcacgacc cctacctgct
     6781 ctttatggaa agctcgctgc tgatccgagc catccgcgac tatttccgcc ccgacatcgg
     6841 cgagattttg gtggacaatc aagaagttta cgaccaagtt gccgagttca tgagctacgt
     6901 catgccgggc aatataggcc gtctgaaact ctacgaagac cacacgccgc tgttttcccg
     6961 cttccaaatc gaacaccaaa tcgaaagcgc gttttcgcgc agcgtcagcc tgccctccgg
     7021 cggcgcgatc gtcatcgacc ataccgaagc cctcgtctcc atcgacgtga actccgcacg
     7081 cgccactcgc ggcgcagaca ttgaagacac cgcgttcaaa accaatatgg aagccgccga
     7141 agaagtcgcc cgacaaatgc gcctgcgcga cttgggcggc ttggtcgtca tcgacttcat
     7201 cgacatggaa aaccccaaac accagcgcga tgtggaaaac gtcctgcgcg acgcgctcaa
     7261 aaaagaccgc gcccgcgtgc agatgggcaa actctcccgt ttcggacttt tagaattgag
     7321 ccgccaacgt ttgaaaccgg ctttgggcga aagcagccac gtcgcctgtc cgcgctgcgc
     7381 cggcaccggc gtgattcggg gcatcgaatc caccgccctg cacgttttac gcatcattca
     7441 agaagaagcg atgaaggaca acaccggaga agtgcgcgca caagtgcccg tcgatgtcgc
     7501 caccttcctg ctgaacgaaa aacgcgccga gctgtttgcg atggaagagc gtttggatgt
     7561 caacgtcgtc ctgattccga acatccacct cgaaaatccg cactacgaaa tcaaccgcat
     7621 ccgcaccgac gacgtagaag aagacggcga accgagctac aaacgcgtcg ccgagccgga
     7681 agaagacgaa tccgccaaac cgttcggcgg cgaaaaagcc aaagccgccc gtcccgaacc
     7741 cgccgtcaaa ggcgtgcgcc acaccagccc cgccccgact gccgcccccg agaaaaaaac
     7801 ctcttggtgg gacagcttca aagcatggct caaacgcatt ttcggcggca gcgaaaccca
     7861 agccgcgccc gctgccgaaa cctccgaaaa acgcagcacg gcaaaccgca gcggcagccg
     7921 cgccaacaac cgccgccaaa acccgcgccg cagcaaacgc gaaggcagca aagtagaagt
     7981 ccgcgaagtg gcaggcaaaa ctgccggaca ggaagcgcgt gccgacaaag ccgaaacgcg
     8041 caacaacggc aaccgccgcc gcaatgaacg cggcgaccgt gccgccgaac gcgccaacga
     8101 agcggaaatc caaagccgca acgtacagcc tgccgcaacc gttgcagatg ccgcaccgtc
     8161 cgaaaccgaa atgcaaaccg gcaaacgccg ccgcaacggc agccgcagcg aacgcagcca
     8221 aaccgcgccg gaaaccgccg ccgttgccga aacagccgtt cagacagcgg aaaacacgcc
     8281 gtccgaaccg cataccgcag aagacaaagg cagcaagccc aaatccgaac gcaaccgccg
     8341 cgaacgcgac agccgcgacg ccaaagaacg ccgcgagcgc aacaatcagc gcgaccgccg
     8401 tcaaaacggc aaaaaacgca atatcccgtc tgccgccaaa atcgagcagt acctgaatat
     8461 tcacgacacc gccgacaaag tccgttccgc cgccgcgcac gttttcggcg aaaccgacgc
     8521 aaacgcgccg attaccgtca gcattgccga tccggttgca gaaagggatc ttccgacagc
     8581 atctcccgcc gtttcaaacg gcgacgcacc ggtttatgat gcggcggaaa aaatccgccg
     8641 tgccaccgcc gccatcctgc ccgaaggcgc gacaccgaaa gccgaagcac aggaaatgcc
     8701 gtctgaaacc gcaaccttta cggctgcggc ggaacaggca cgggaaaccg cacaaaccgg
     8761 cggactcgtc ctgatcgaaa ccgaccctgc cgcattgaag gcatgggcgg cacaacccga
     8821 agtccaaacc ggacgcggtt tgcgccgttc cgaacagccc aagccgtctg aagtcgcaac
     8881 cgtccctgcc gaagaaatga tccaagtcga aacccggcaa ggctgaaccg acggcggcaa
     8941 aaagaggttc tgttccgcag aacctctttt ttatatagat tcggatacct gcaatgccgt
     9001 ctgaagcctc accattccca tgattaccgc aacctttcgt cattcccgtg aaaacgggaa
     9061 tctagaacct caaactttca aataaccttt gaatattgcc gctgccttaa ggcctggatt
     9121 cccacctgcg cggaatgacg gtttagaagt tgcccgaaac ctcaaaaaaa actgaaaccg
     9181 aacaagccgg attcccgcaa cattccgtca ttcccgtgaa aacgggaata tagaacccca
     9241 aactttcaga taatctttga atattgccgt cgcccaaagg cctggattcc cgcctgcgcg
     9301 ggaatgacgg cggagggtgg acgatgccgt ctgaaacttc gccattccca tgattaccgc
     9361 aacctttcgt cattcccgcc acctttcgtc attcccgtga aaacgggaat atagaacctc
     9421 aaactttcag ataatctttg aatattgccg ccgcccaaag gtctggattc ccgcctgcgc
     9481 gggaatgacg gcggagggtg aacgatgccg tctgaaactt caccattccc atgattaccg
     9541 caacctttcg tcattcccgt gaaaacggga atctagaacc tctaaacttt cagataatct
     9601 ttgaatattg ccgtcgccca aaggcctgga ttcccgcctg cgcgggaatg acggtttaga
     9661 agttgcccga aacct
//
LOCUS       DAWGJV010000070         9648 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWGJV010000070 DAWGJV010000000
VERSION     DAWGJV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.070"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1..1183)
                     /locus_tag="WHQ94_001236"
                     /pseudo
     CDS             complement(1..1183)
                     /locus_tag="WHQ94_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1329..2033
                     /locus_tag="WHQ94_001237"
     CDS             1329..2033
                     /locus_tag="WHQ94_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ2128510.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2097..2663)
                     /locus_tag="WHQ94_001238"
     CDS             complement(2097..2663)
                     /locus_tag="WHQ94_001238"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ2128511.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2729..3145)
                     /locus_tag="WHQ94_001239"
     CDS             complement(2729..3145)
                     /locus_tag="WHQ94_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128512.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVSANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIQEVKLPKNK"
     gene            complement(3197..4096)
                     /locus_tag="WHQ94_001240"
     CDS             complement(3197..4096)
                     /locus_tag="WHQ94_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ2128513.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(4127..5584)
                     /gene="der"
                     /locus_tag="WHQ94_001241"
     CDS             complement(4127..5584)
                     /gene="der"
                     /locus_tag="WHQ94_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ2128514.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5738..6367)
                     /locus_tag="WHQ94_001242"
     CDS             complement(5738..6367)
                     /locus_tag="WHQ94_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ2128515.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6368..7663)
                     /gene="hisS"
                     /locus_tag="WHQ94_001243"
     CDS             complement(6368..7663)
                     /gene="hisS"
                     /locus_tag="WHQ94_001243"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ2128516.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7789..8331)
                     /locus_tag="WHQ94_001244"
     CDS             complement(7789..8331)
                     /locus_tag="WHQ94_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ2128517.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVRQIRQARAE"
     gene            complement(9448..9648)
                     /locus_tag="WHQ94_001245"
     CDS             complement(9448..9648)
                     /locus_tag="WHQ94_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128518.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2275 a         2318 c         2449 g         2606 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgttgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgtttag acgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagcagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttggggaggt actgccattc gcagccgtat ttgtgttgca ggacgtgtgc
      661 gaaatggcgg ctgatggcga gacgtgcggc ggcgtgcgcc gccgcatttt tcataggctg
      721 ccattggtgg gggcggatca gaccgcgcgt aatggtgctg ctgtgtttcg tgacgacata
      781 ggggatgccg tatttttggg aaattttttg ggcgagtatg ccggcatagt tcatgcggtg
      841 ggcgtgaatc aggtcgggca gcccgttttc gcggatgtag tgtttgaagg ctttcaaacc
      901 cacgcgcacc cagcggatgc ggtcgatgtc gatgaacgga aagcggggga agaaatatat
      961 gccgtgccat gcatagatgt ccaaaccgct ttgccgatat tttgcaaaac cgtaaggtcg
     1021 gtcaggatgc ttgctgtttc tttccgcaag tagcggaaca tcggtgcaag cacggcggtt
     1081 ttgatgcctt tcctctgcaa ttccagtgcc tgattttgaa aaaaatcccg tccacatcct
     1141 gttcggattg cggataccat gaggggatga cgaggacgtg caagggtttg ggcatagtgg
     1201 gattccgtat cggaaaggcg gttattataa gacagacgca gaccgaatat ttaaattgtt
     1261 gccttacgct aatgcaattt agcgcgccga tatgctagat tggcggtttt atgagtgagg
     1321 atacggatat gttgcgttct attttggcgg cttccctatt ggcggtatct tttccggcag
     1381 cggctgaagc attgaattac aatattgtcg aattttccga atcggcgggt gtcgaggtgg
     1441 ctcaggatac aatgtccgca cgtttccaag tggcggcgga aggacgggac aaaaatgccg
     1501 tcaatgccga gtttgttaaa aaattcaaca gttttaacag aaaattgaaa aacggcagct
     1561 ttaaaaccga attggtatcg cgcagtgcga tgccgcgcta tcaatatacc aacggcagac
     1621 gcattcaaac aggctgggag gagcgtgcgg aatttaaggt cgaaggtagg gattttgatg
     1681 cgttgaaccg ttttattgct gatgttcaga cggatgcttc gcttgaatat accgatttca
     1741 gcgtgtcgcg tgaacgccga aacgaggtca tcgatcaggt cagcaaggat gctattttgc
     1801 gttttaagac gcgtgccgaa aaactggcgg gcattttggg tgcgtccggt tataaaatcg
     1861 tcaaattaaa tttgggacac atcggcagcc atatcgcggg agggggagct gctcaggcaa
     1921 aaatgcttcg tgccatgccg atggcggcaa gcgtcaatat ggagggtgcg gattctgccg
     1981 cgcagggtgt ggaggaaatc agcatcagcg tcaatgggac ggttcagttc taaccacgga
     2041 taaacaggta gatgccgtct gaaaccggat gatacggttc agacggcatt tatatttcag
     2101 gctttaggca gggtaacgcc ggtttgcccc atatatttgc cgttgcggtc tttgtatgag
     2161 gttttgcaga tttcgtcgct ctcgaagaag aggacttgcg ccacgccttc gccggcgtag
     2221 attttggcgg gcaggggggt ggtgttggaa aactcgaggg taacgtagcc ttcccattcc
     2281 ggctcgaacg gggtaacgtt gacgatgatg ccgcagcggg cgtaggtgga tttgcccaag
     2341 cagacggtca ggacgttgcg cgggatgcgg aaatattcga ccgtgcgcgc cagtgcgaag
     2401 gaattgggcg ggatgatgca gcagtcgtct tcaacggtaa cgaagttttt cgggtcgaag
     2461 tttttgggat cgacgatggt gctgttgatg ttggtgaaga ttttaaattc gtttgcgcag
     2521 cggatgtcgt agccgtagct ggacgtaccg taggagatga tgcgtttgcc gtcggcttct
     2581 ttgatttggt tcggctcgaa agggtcgatc atgccgaatt cttcgctcat tcggcgtatc
     2641 catttgtcgg acttgatgct cataatgttt tccttgtttc ttgcggtgtt cggacaaagc
     2701 attcgggaat gccgtctgaa aacggggctt atttgttttt gggcagtttc acttcttgaa
     2761 tcatgccgtt ttcacatttc ataatgaaga gggtttcgcc gttttgcgag acggtaaacc
     2821 tgccgtgtgc caggcctttt ttgaacgtgc ccgagagtac catgttgcgg aatttggtac
     2881 tgtcggaatt gaacggttcg ataaatattt cgcggttggc ggaaacggta taaacgcctt
     2941 gtccgtcgaa tttgccattt ttaaacgaac cggtataacc gcgcccgtcc cggcaacgcc
     3001 atatgccttt gcctgaagga agcccgtctt ttccgactcc gccgtcgtag gtgcagccgg
     3061 gttcttgata ggaagtcaga acggcggccg aagtggggag ggcgaacatc atggcgggca
     3121 gtaggaatgc gagatgttta agcataaggg ttattccatt ggattttggc tgacggtata
     3181 aaagccgtct gaaaaatcaa tcttgccagc cgcccaaata ggaaaccagt tcttccaaca
     3241 tggtgctgac ggattccgcc atcagaattt gcgaggcgaa ggcaaggccg gcggcatcgt
     3301 cgccgttgct ttcggcttct tcctgcaata cgtcgaggta ttggatgcgc ttgagtgtga
     3361 agtcttgagt gaggataaag gcaatttgtt cgcgccacac caagcccagt tgggtaacgg
     3421 ttttaccgtt tttgacgtgt tgaaccactt cgtcggcggt taaatcttgt ttggatactt
     3481 tgacgacggg aacaatatcg cccgtacctt tgagttcgca atcgctgtct aattcaaaac
     3541 cgccttcgca atgcccttgc aacagccagc cggtcatcaa ggaagagggc gattgcttgg
     3601 tattcggcag cgaggcttcc aaaccgccca aagcttcgcg cagcttggtc aggatgtttt
     3661 ctgctttggc ggaagccgcg ttattgacga gcaggtagcc gtggcgggtg ttaaacaccg
     3721 cttctgtacg gctgctgcgg gtaaacgctc ggggcagcag gtcgtctgta atttgctctt
     3781 taagctcttg tttttcttta cggccgacat tgcgggcttc attgttttgg atttccgcta
     3841 ccttctcttc caaaatatcg cggatgacgc cggcaggcag gactttttct tcttttttca
     3901 gggcgacgcg caaggtaaag tcggcaggga aaacgagttc aggggagaat gaaaccggtg
     3961 cggtaaagcc ttcgctgaac cagtctaagc cttggcaatg ggtaaattca gcttcagcaa
     4021 gtttgtcggc aagtacgtct gcctcaggca gcttttcttt gttgagcgga taaaaactaa
     4081 tctgcttgaa ccacataatg tttcctattg tttgaaatgt cgggaattat ttgctgaatt
     4141 gttttttcac actgactttg gttttcttct tgaagcggtt tttctcttct ttgcggcctt
     4201 cgcgtttctc aatacggttg ctcaggctga cgcggcgcag cggttttttc ttcggtttgt
     4261 cttccgcatt ttcatacggg ttttccgaaa cattgtattg aatccgcaac ggcgtgcctt
     4321 gcaggttgaa ggctttgcgg aacgtttggg tcagatagcg cgtatagctg tcggaaatcg
     4381 cgtgcagcga attgccgtgt accacaatta cgggagggtt catgccgcct tggtgggcat
     4441 aacgcatttt cggacgcacc aagccggcac gcggcggttg ctgacgctcg atcgcgcttt
     4501 gtaatacgcg cgtgattttc ggcgtcggca tcttaatcat cgccgcgttg taggcagcct
     4561 gaatgctgtc aaacaaaccg tctataccgc gctctttcaa tgcggaaata aagtggaact
     4621 tggcaaaatc gaggaaatac agtttgcggt tgatatcgcg tttcacttgc tcgcgacgct
     4681 cttcgctgat gccgtcccat ttgttgacgg caaccaccaa ggcgcggcct gcttccaaag
     4741 caaaaccggc aatcgtcgca tcttggtcgg cgatgtcctg ctgcgcgtcc aataccaaaa
     4801 cagcgacgtt tgccgcttca accgcctgca tcgctttgat aacggagaat ttttctactg
     4861 cttcatccac tttgccgcga cggcgcacgc ctgcggtatc gatgatggta aacggtttgc
     4921 cttcgcgttc gaaatcgatg tggatgctgt cgcgtgtcgt gcctgccata tcgaaagcaa
     4981 tcacgcgctc ttcgccgagg atggcgttaa ccagtgtgga tttgccgacg ttcggacggc
     5041 cgatgacggc aaagacggga tgttttgcat cggcttcttc ggcttccggc tctgggaagg
     5101 tttccaaaat atcttcaatc agataataca cgccatcgcc gtgcgcacct gaaataacat
     5161 aagggtcgcc caaagcaagt tcgtagaact cggcggcaag cacggccctg ttgccgcctt
     5221 cgcctttgtt taccgccaga taaacagggc gcgggctttg gcgcaggcgg tcggcaatga
     5281 ttttgtcttg cggggtcagg ccggtacggc cgtccaccaa aaacacaacc gcatcagctt
     5341 catcgacagc ctgcaaggtt tgttttgcca tttcgtgcaa aatgccgctg tccacaaccg
     5401 gctcgaaacc tccggtatcg atgaccaaat aaggtttgct gccgactttg ccgtgtccgt
     5461 aatggcggtc gcgggtcagg ccgggcaggt cgtgtacgag tgcgtctttg gtgcgcgtca
     5521 aacggttgaa taaagtagat ttgccgacgt tagggcggcc gacaagcgcg atggtgggtt
     5581 tcattattgg gtctttctgt gtcaagtgcc gttcgggaga actgaacacg agcaggtgtc
     5641 cgttggacac ggtaaatagg ttcttcacgg caagaggcca taaagaggga gggtaaaatg
     5701 ccgtctgata aataatggac agcatttcag acggcattta tttcagcgaa tcaagtttca
     5761 tttgaaccaa ttcgcgaccg acagaatctt gaggcatttt ttctaaagcc tgtccgtagt
     5821 tttttaaggc ttcctggctt tttccctgtg cggcatagac atcgcctttg gtttccatca
     5881 gcaggggggc gaagtccgct tcaaccggcg tatcgagcgc ggcaagcgcg gcatcgtatt
     5941 ttttttgttg caacaacaca acgcccagac gctgcgccgc caacgcttga atcaggctgt
     6001 ctttttggtt ggacaacacc catttcaaat ggccttcggc aacatcgtaa cgctgtgcgt
     6061 caaattcggt tgccgccgcc atcagcgtgg cttgggcggt ggaaatggaa tgcgggtagc
     6121 tttgctggag tttggtcaat tcggcattga tttcgctttg cggggctttg ttttgcgcct
     6181 tttccacgat gtttgccagc accgccgccg cttcctgatt ttgggaagtc gcacggtttt
     6241 ggtaaaccgt gtatcccaag tagccgagcg ccgccaaaat cagcagggca aacagccatt
     6301 tgcccgtggt tttccaaaaa tatttaaagt tgtccaactc ttgttgttct tccaaatggg
     6361 ctgccattta tgcgttcttc cattgttgta aagtgtttgt caaatcagcg gcggcgacag
     6421 tttgctgacc gtgtgcgccg ttcatgtctt tgagcgtaac cgtaccgttc gccagttcgt
     6481 cttgcgccac aatcagggca aagcgcgcgc cgctgttgtc ggcttttttc atttgtgctt
     6541 tcaggctttg atagccggaa tgctgcatta cattgaaacc ttgcgcgcgc aaggcttgcg
     6601 cgtatttcat cacctgcaag tccgcccttt cgccttggtg cattgcatag acatcaggcg
     6661 cagcgttcac ttccagcgag ccgtattcgc tcaccaaaag cagcagccgt tcgatgccca
     6721 tcgcaaagcc gatggacggc gcaggtttgc cgccaagttc ctcaatcaaa ccatcgtaac
     6781 ggccgccgcc gcacacagtt gcctgcgcgc cgagtttgtc ggtcgtccac tcaaaaaccg
     6841 tctgattgta ataatccaaa ccgcgaacca agcgcggatt ttcaatatat tggataccca
     6901 aaccgtccaa catcgccttg aagcgtgcat agtggttttg cgaagcctcg cccaaataat
     6961 ccaccaaacg cggcgcggcg ttgcagattt cctgcaaatc agggtttttc gtatctaaaa
     7021 cacgcaaagg attggttttc agacggcgtt tgctgtcttc gtccaattta tcttcatagc
     7081 gtgtcaaata ttcaaccaac gccgcacggt gtgccgctcg ctcctcacgg ttgcctaagc
     7141 tgttgatttc caaagtcagg tattcgcgga tacccaattt ttcccacaag tcggcagaca
     7201 tcgcgatgat ttccgcatcg atatccggcc cttcaaaacc caaagcctcg ataccgacct
     7261 gatggaattg acgataacgg cctttttgcg gacgctcgcg gcggaacatc ggccccatat
     7321 accacagctt ttgcgggctg ttgtacagaa ggttgtgttc gaccaccgca cgcagacaag
     7381 aagccgtacc ttccggacgc aggctcaaac tcaaagaatc gtttgaatcg gaaaaggtgt
     7441 acatttcctt gccgaccaca tcggtttctt cgccgatgga gcggacaaac aaaccggttt
     7501 gctcgacaat cggcgtacgg atttgctgat agccgtaagc gcgcgtccag cggtttaccg
     7561 tatcctcaaa cgcctgccaa aacgcagccg tcagtttgaa atctttttgc tcgacaggca
     7621 gaaggtcgtt catgcctttg acggattgga ttttttgtgc catttcaagt aagaatgctt
     7681 aaatcaaatt gcgggcgatt atagcggatt tttaagggtt tgtgaggttg ggggtggttt
     7741 gcggacgggt aaaatgccgt ctgaaacagt tttcagacgg catttgactt actctgcacg
     7801 tgcttgcctg atttgtcgga ctgcaaactc tgtctgccgc ttgggatgat gtgtgaaaaa
     7861 caatttattt gaaattgtct cggctttttt cggcacgacc gccaaaatct tacctgccaa
     7921 atttccctca cgggtttgcc aagcatccaa aaactgcgcc ctgctcatcg aaacatgacc
     7981 cagcgacggg tcggcaagca aaaccgtatt gccgtctata ccgcgcaata ccgagaaatg
     8041 atcatcctta cggtatttca gatacacgat gacggggatt ttcaactgcg cgagctgctc
     8101 gaaagacagg gcatagcctt tcgcttcaaa acccaaatct ggcataatgc gccgcatatc
     8161 ctcaaacgac gcgcgcatct gctccttatc cagctttttc aacacttctt cttccgtcag
     8221 cgtttgcccg taaaaattgt tcaaaagcgt cgccaccgaa gccgccccgc aggaaaaatc
     8281 caaatcctgc tttacaatat tgaaatcccg ccgcgctttc caactctgca ctttgatttt
     8341 tccgtaaaca acaggattat cgttaaacat cggtgcagca ttcagacgat aagataaaga
     8401 aacgacaaca cacgccaaca gaaaaacata tttgaacttc atcatattgt ccacataaag
     8461 ggcagcctga aaatctttca ggctgccctt gtcaaattat tcctagcttt cggctttttt
     8521 ggcaaaccaa acaatccgat tacccgcata atactttcca tttattgaaa tccgacaagc
     8581 cgcgcccaaa aatgccatgc actgtcgatt tccgcagcaa tctttgtacc gttttcttca
     8641 aattccaaat attcacccaa taataaactt gaaacagaac gcgtgggcag caagtgccca
     8701 ccctacgctt attcaaataa tttgattaaa taaagaataa agaaaatgca tcaggaacaa
     8761 aattataatc tgccacctga ctcacaccgc ttttaaagta aggggcatca aaatcaaaac
     8821 cgcaaaaaaa ataatttttg cattgatttt taatagattt aaaattcgaa tatagtgttt
     8881 ctccagattt aaaaagttta tctttatcta tatatttgat gctttcccta tccaaaataa
     8941 atattcaaac attaaaaaat cattacatga ccaagccaaa tcataaattt gattcaaatg
     9001 ggtatcataa agataaaaat aataaggttt gggaacaggt aaaatattta atggaaaagg
     9061 aggactaatt ttcttaatat ctgatgactg atatccatat ctttctatat tcttaaaaat
     9121 ttcatctttc ttaagataac aagacattct gaagataaca agacattctg acaatcacaa
     9181 cctgttcatc ggatatatta tctatttgca tggcgcgtat aacacgccat gcctgattaa
     9241 aattagtctc ccttacctta gaatctatta tcttttccaa agatgaaaat gccccttatc
     9301 ttgaactctc cagttagaac ctggaaattt cgtacctctt aaatgtgtat taatatttct
     9361 tatagtttca ttaaaatgcc acgcgctgcc gatttcaacg gtaattttcg taccgctttc
     9421 ttcaaattcc aggtattccc ccattagcta acgcaaagaa gcagacgcca tttcggcttc
     9481 gttatgataa acccgccttc cgttgatata gacttccgcc cctgtccgct ccaaattcca
     9541 aaaattccgt actgaaattt ccatatcccg atattgtgca gaccatgttt tttcgaaggt
     9601 tttcataaaa tttcctatac ctgtccaatc ggcacatatc aattgcat
//
LOCUS       DAWGJV010000071         9640 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWGJV010000071 DAWGJV010000000
VERSION     DAWGJV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.071"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(137..1183)
                     /gene="adhP"
                     /locus_tag="WHQ94_001246"
     CDS             complement(137..1183)
                     /gene="adhP"
                     /locus_tag="WHQ94_001246"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ2128519.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLAVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVINAA
                     KEDAAKVIQEKTGGAHVAIVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1372..1761)
                     /locus_tag="WHQ94_001247"
     CDS             complement(1372..1761)
                     /locus_tag="WHQ94_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ2128520.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFKITLSEVSPDHFTLKADPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            1987..3165
                     /locus_tag="WHQ94_001248"
     CDS             1987..3165
                     /locus_tag="WHQ94_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ2128521.1"
                     /translation="MEKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESSERALGGPPRR
                     "
     gene            3231..5165
                     /locus_tag="WHQ94_001249"
     CDS             3231..5165
                     /locus_tag="WHQ94_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ2128522.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5483..6265)
                     /locus_tag="WHQ94_001250"
     CDS             complement(5483..6265)
                     /locus_tag="WHQ94_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ2128523.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6404..8593
                     /locus_tag="WHQ94_001251"
     CDS             6404..8593
                     /locus_tag="WHQ94_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ2128524.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPQGMRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMASLKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKVQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8640..9534)
                     /locus_tag="WHQ94_001252"
                     /pseudo
     CDS             complement(8640..9534)
                     /locus_tag="WHQ94_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
BASE COUNT         2233 a         2493 c         2807 g         2107 t
ORIGIN      
        1 cactataatg acgtgttgcg atacgggttt tcagacggta tttcaaacag aacagattaa
       61 aatcaaatcc aaatccataa aaaatgccgt ctgaacagcg ttcagacggc atttcgatgt
      121 gtatcgccac gtcaaatcag tggtgatgac cgcagccgca ttcttttttc atatcgatca
      181 ccatacggcc ggtgattttg ccttcgcgca tttcttggaa aatggcgggt gcttcatcca
      241 aagcacgcag ttggactttc ggcacaacca aaccttccgc gccgaattgg aaggcttctt
      301 ccaaatcttt gcgcgtgccg accaaagagc cgaccacttc gatgccgtcc aaaaccaaac
      361 gcgggatgga caaatccatc gattccggcg gcagtccgat ggcaaccaca cgtccgcccg
      421 cgcggacgca attcacggca gagttgaatg cggcggcgga tacggcggta acaatggcta
      481 cgtgtgcacc gccggttttt tcctgaatca ctttggcggc atcttctttg gcagcgttga
      541 tgactaaatc tgcgccgctt tctttggcaa aagccagttt ttcatcgttg atgtcgatgg
      601 cgacgacgtg tgcgccaaat acttttttcg cgtattgaac cgccaagttg cccaaaccgc
      661 ctgcgccgta aatcgcaatc cattggcccg gacgaacgcc ggaaacttta atggctttat
      721 aagtggttac accggcacaa gtaatgctcg aagcttgcgc aggatccaag ccttctggga
      781 ctttgaccgc gtaatcggca ctcacgatac agtgggtcgc cataccgccg tcggcggtgt
      841 agcccgcgtt caatacagag cggcacaagg tttcgcggcc ggtattgcag tattcgcacg
      901 agccgcagct ttggaacagc caggcgatgc tgacgcggtc gccgactttc agatttttca
      961 caccgtcggc aacttcttta accaaaccga tgccctcgtg tcccagcacg cggcccggtt
     1021 tttcgccgta gtcgcctgcc gcaacgtgca ggtcggtgtg gcacacgccg caatattcga
     1081 cttcgaccaa cgcctcgccg tattccaacg ggcgaacctc gcgttcgatt acttccacat
     1141 cgcccgctac atttttattc acaacaactg cctgcatttt catgattgcg ctccttagtt
     1201 acggcaaaaa acctgtaaac ggaatgttgt ccgatataag ggtaagcata cgcttccgca
     1261 tctcacaggt caagtggtat gttgttgaaa aatatgggtt atatgttata ttataacagt
     1321 tggggaggca cagcatcggg gcggttgccg gatgcgggct tcggcggagg attagtcgaa
     1381 gccggggcag gattggtttg taccggaagc ggcaatggta ccgccgtcgt tgagcgtaac
     1441 gacgcaggtt tcgccttcgt tggtcatcgg atcggggtcg gccttaaggg tgaagtggtc
     1501 ggggctgact tcgcttaagg taatcttgaa atattcgttt tgtttcagtt tgtttttgtc
     1561 gtaggtttta aacgtccctt tttggcggta gtaacgttcc atggtctgcg cgttgtgcag
     1621 cagggtcgtc ctgacttccg acaggcggac gcgccggatg taggttttat aggaagggta
     1681 ggtgatgagc gtcaggatgc cgaggatggc gacggcaatc atcagctcga gcagcgtaaa
     1741 gcctttttga acgtttttca tagcaatgtg tttccatttg tttgtcggtc ggcgacatta
     1801 taacgccgta tcggaaatgg cggaatatgt aaacggattg aaattttcgg gaaagcagat
     1861 tgtataagcc atttaaaaca aatggttatt tttattgtcg gcagtttgcc gccttggatg
     1921 gggcagggac ttgcggtaga atccgcttcc gatttatggg attgacgcat acagagaatt
     1981 gaaaacatgg aaaaaatgat gaaatgggcg gctgttgcgg cggtcgcggc ggcagcggtt
     2041 tggggcggat ggtcttatct gaagcccgag ccgcaggctg cttatattac ggaaacggtc
     2101 aggcgcggcg acatcagccg gacggtttct gcaacagggg agatttcgcc gtccaacctg
     2161 gtatcggtcg gcgcgcaggc atcggggcag attaagaaac tttacgtcaa gctcgggcaa
     2221 caggttaaaa agggcgattt gattgcggaa atcaattcga cctcgcagac caatacgctc
     2281 aatacggaaa aatccaagtt ggaaacgtat caggcgaagc tggtgtcggc acagattgca
     2341 ttgggcagcg cggagaagaa atataagcgt caggcggcgt tgtggaagga tgatgcgact
     2401 tccaaagagg atttggaaag cgcgcaggat gcgtttgccg ccgccaaagc caatgttgcc
     2461 gagctgaagg ctttaatcag acagagcaaa atttccatca ataccgccga gtcggaattg
     2521 ggctacacgc gcattaccgc aacgatggac ggcacggtgg tagcgattct cgtggaagag
     2581 gggcagactg tgaacgcggc gcagtctacg ccgacgattg tccaattggc gaatctggat
     2641 atgatgttga acaaaatgca gattgccgag ggcgatatta ccaaggtgaa ggcggggcag
     2701 gatatttcgt ttacgatttt gtccgaaccg gatacgccga ttaaggcgaa gctcgacagc
     2761 gtcgaccccg ggctgaccac gatgtcgtcg ggcggctaca acagcagtac ggatacggct
     2821 tccaatgcgg tctactatta tgcccgttcg tttgtgccga atccggacgg caaactcgcc
     2881 acggggatga cgacgcagaa tacggttgaa atcgacggcg tgaaaaatgt gctgattatt
     2941 ccgtcgctga ccgtgaaaaa tcgcggcggc aaggcgtttg tgcgcgtgtt gggtgcggac
     3001 ggcaaggcgg cggaacgcga aatccggacc ggtatgagag acagtatgaa taccgaagta
     3061 aaaagcgggt tgaaagaggg ggacaaagtg gtcatctccg aaataaccgc cgccgagcaa
     3121 caggaaagca gcgaacgcgc cctaggcggc ccgccgcgcc gataaacgaa tatgccgtct
     3181 gaacacggaa acggtttcag acggcatttg ttattgattt acggaatatt atgagcttga
     3241 tcgaatgtaa aaacatcaac cgctatttcg gcagcggcga gaaccgcgtc catattttga
     3301 aagacatcag cctgtcgata gagaagggcg attttgtcgc catcatcggg cagtccggtt
     3361 cgggcaagtc cacgctgatg aacatactcg gctgtttgga taccgccggt tccggttcgt
     3421 accgaatcga cggcatcgaa actgccaaaa tgcagcctga cgagctggcg gcattgcgcc
     3481 gcgaacgctt cggtttcatc ttccaacgct acaacctctt aagctcgctg accgcaaggg
     3541 ataacgtcgc gctgccagcc gtctatatgg gcgcgggcgg caaagagcgt tccgcgcggg
     3601 cggacaaact cttgcaggat ttgggtttgg caagcaaaga gggcaacaag cccggcgaac
     3661 tctcgggcgg acagcagcag cgcgtctcca tcgcccgcgc cctgatgaac ggcggagaaa
     3721 tcatcttcgc cgacgagccg accggcgcgc tcgataccgc cagcggcaaa aacgtgatgg
     3781 aaatcatcca taagctgcac gaagccgggc ataccgtcat tatggtcacg cacgaccccg
     3841 gcatcgccgc caatgccaac cgcgtcatcg aaattcggga cggcgaaatc atttccgaca
     3901 cctcgaaaaa tccagaaatc cccgcaagca atgtcgggag gattcaggaa aaagcctcgt
     3961 ggtcgtttta ttacgaccag tttgtcgaag ccttcagaat gtcggtgcaa gcagtattgg
     4021 cgcacaaaat gcgttcgctt ctgacgatgc tcggcatcat catcggtatc gcttcggttg
     4081 tctccgtcgt cgcattgggc aatggttcgc agaaaaaaat ccttgaagac atcagttcga
     4141 tagggacgaa caccatcagc atcttcccgg ggcgcggctt cggcgacagg cgcagcggca
     4201 ggattaaaac cctgaccata gacgacgcaa aaatcatcgc caaacaaagc tacgttgctt
     4261 ccgccacgcc catgacttcg agcggcggca cgctgactta ccgcaatacc gacctgaccg
     4321 cttctttgta cggcgtgggc gaacaatatt tcgacgtgcg cggactgaag ctggaaacgg
     4381 ggcggctgtt tgacgaaaac gatgtgaaag aagacgcgca ggtcgtcgtc atcgaccaaa
     4441 atgtcaaaga caaactcttt gcggactcgg atccgttggg taaaaccatt ttgttcagga
     4501 aacgcccctt gaccgtcatc ggcgtgatga aaaaagacga aaacgctttc ggcaattccg
     4561 acgtgctgat gctttggtcg ccctatacga cggtgatgca ccaaatcaca ggcgagagcc
     4621 acaccaactc catcaccgtc aaaatcaaag acaatgccaa tacccaggtt gccgaaaaag
     4681 ggctgaccga tctgctcaaa gcgcggcacg gcacggaaga tttcttcatg aacaacagcg
     4741 acagcatcag gcagatagtc gaaagcacca ccggtacgat gaagctgctg atttcctcca
     4801 tcgccctgat ttcattggta gtcggcggca tcggcgtgat gaacatcatg ctggtgtccg
     4861 ttaccgagcg caccaaagaa atcggcatac ggatggcaat cggcgcgcgg cgcggcaata
     4921 ttttgcagca gtttttgatt gaggcggtgt taatctgcgt catcggcggt ttggtcggcg
     4981 tgggtttgtc cgccgccgtc agcctcgtgt tcaatcattt tgtaaccgac ttcccgatgg
     5041 acatttcggc ggcatccgtt atcggggcgg tcgcctgctc gaccggaatc ggcatcgcgt
     5101 tcggctttat gcctgccaat aaagcagcca aactcaatcc gatagacgca ttggcgcagg
     5161 attgaggttg gcacaaagat gccgtttgaa gctgccggac cggtcatttt ggagcagaaa
     5221 cttatcggac aaaacggttt cttagattct acgttccaga ttcccacttg cgtgggaatg
     5281 acggcggtgt gtttcgtatt gttcaattta ttattttcaa tcattcaatg ggttaggatg
     5341 tgtttgttgg ctttctgtac tggtttaaat ttaatccact ataaatttaa tccactataa
     5401 ctttcagtgc ggattggttt tcaggcggca tttcccgcaa aaaaggcttg gaattttcca
     5461 agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc agttggggca
     5521 tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc gtgccgttga
     5581 agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag atgctgccgc
     5641 cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg tcgggctgac
     5701 accataagat ttgcgccttg cgcgcggcat cggggtgcag gccggcaatg ggcatcataa
     5761 agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt ttgcagaacg
     5821 gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc acttctttga
     5881 tggctttgtc caaaggcagg gaggcgaagt cgattttgtt caaatcggcg gcgcgttctt
     5941 cggtcaggtt tttgcgcgtg tcgatgttga tgagttcgcc gacgaacata tagccgcctt
     6001 cggcatcggt gtagataatc tgcctgccgc tgacgacgac ttcgtaaatg cctttgaccg
     6061 gtgtttcgct aacgctcaac actttcaaat cttgggcgga ataggttttt tccaaacgcg
     6121 ctttcaaaga ggcggcaacg gatttgccgg cggactcggc tttggcggcg ggttcggcgt
     6181 tggcattgga aacgggcgtt tgcccgcaag ccagcagcgg gaggacggta aagggggtca
     6241 agattttgat taacttggtt ttcatataaa gatgattgcg cgtgttggaa aagcggaatt
     6301 gtatcaaatc tctgttgcgc ctgcattgcg cctaggctca atttatcgtc tgaaaatagc
     6361 ttccgcctgt taaaatacgc aaaaaaatga tttgcctgcc tgtatgatct accaccgtat
     6421 cgccgtaaac gtgccgcttt cagacggtct tttaacttat tcccattccg agccgctgcc
     6481 gcagggaatg cgggtgcttg tgccttttcg caataaaacc gtggtcggga tggtgtggga
     6541 aacggatatt gcgccggata tggatacggc gcggattttg agtattcaga cgaccttttt
     6601 agacgagccg ccgttgcctg aaagctggcg tgatttgctc tcgtttacgt cgcgttatta
     6661 ccactatccg acggggcagg cggtgtttgc cgcgctgccg cagggtttga aggaaacgcg
     6721 cgcggtggaa atgccgcagc cgccgttgtt ttacgcgctg aacgaagcgg gcagggcgca
     6781 aacgccgccg ccggcgcggt tcaacaaaaa agcggctttg tgggacgcgc tgctgtcggg
     6841 cgaaatgacg atggcatcgt tgaagcaggt aaacgcgcag gcggtgaaat taatcgaaaa
     6901 ttgggcggaa cagggttgga ttgaaacgac ggaagcggcg aagcccgttt taaggtcgtg
     6961 taatgggcag gcttcgcact ctgaatttgt attaaatgct gaccagcaga aggcttccga
     7021 tgccattcag acggcattgg gacgcttcca gccgtttctg ctgtacggca tcaccggcag
     7081 cggcaagacc gaggtgtatt ttgatgcgat ggcgaaagtg ttggctcagg ggcggcaggt
     7141 gttgtttctg ttgcccgaaa tcaacctcac gccgcagctt ttgaagcggg tagaaaaccg
     7201 ttttgccgac gtgccgaccg ccgtgttgca cagccagatg gcggcaggca ggcgcacgca
     7261 agattatttg cgcgcgatgt tggggcaggc gaaattagtc atcggcacgc ggctggcggt
     7321 gttcacgccg atggatgatg tcgggctgat tgtggtcgat gaggaacacg acggctcgtt
     7381 caaacaagac aacgaattac gctaccacgc ccgcgatttg gcggtgtggc gggcgaagca
     7441 gagcggctgc cccgtcgtgc tgggtagcgc cacgcccagc ttggagagct ggcacaaggt
     7501 gcaaagcggc gcgtaccgcc tgctgcaact gaccgagcgc gcccataccg ctgcgcaact
     7561 gccgcaagtg gacatcctca acgtaggccg tctgaaacta gacaatggct tctcgccgca
     7621 agccttgcag cttttgaaac agaattttga agcgggcggt atgtcgctgg tgtacctcaa
     7681 ccgtcgcggc ttcgcgcccg cgctgttttg cggcgactgc ggccatacct tcggctgccc
     7741 gaactgctcc gccaaaatgg tgctgcacca acgcgtccgc caactgcgct gccaccactg
     7801 cgaccaccgc gaacccatac cgttcaaatg ccccgactgc ggaaatcaag acctgaccgc
     7861 cgtcggacac ggcacgcagc gcgtcgaaga aaccctgcgc gccttcctgc ccaaggcagc
     7921 cgtcgtccgc gtggacaggg acagcaccgc gcacaaaaac gactgggcgg atttgtaccg
     7981 ccgcatcgcc gacaacgaaa tcgacattct ggtcggcacg cagatgctcg ccaaaggcca
     8041 tgactttgcg cggctcaacc tcgttatcgt attgaacgcc gacggcagcc tttacagcgc
     8101 ggatttccgc gcgccggaaa gactgttcgc cgagctgatg caggtgtccg gcagggcggg
     8161 gcgcgccgac aaacccggca aggtgttgat acagacccaa ctgcccgaac atcccgtctt
     8221 cgccgccgtc aaagcgcagg attatgccgt gtttgccgaa aacgaattga acgaacggca
     8281 aatgttcgcc atgccgccct tcggttttca gaccgccgtc cgcgcggacg cgccgcgtgt
     8341 tgccgatgcg ctggaatttc tcaatgccgc caaagaaacc ctcgccccgc tgttgcccgc
     8401 aagcgtttcc cagtttggcg ccgcccccat gctgatggtg cgcctcgccg aacgcgaacg
     8461 cgcgcaaatc ttcctcgaat ccccgtcccg acaggatttg caccgtgccg tgagtttgtg
     8521 ggtgcaggtg ttgcagcaaa accgcgacgg caaaattaga tggtcggtgg atgtcgatcc
     8581 gcaggaggct tgattatcgg caatacgatg ccgtctgaaa accgtttcag acggcatttt
     8641 tattccggat cgtctgtaaa cgcgttcgcc cgaaagatcg gtacgaatgt cagcaggtaa
     8701 agcacgaaca cggcggcggc gaaaaggatg gggacggcga tgcggcagag tttcgggtag
     8761 tcgagcttgg taaagccgct gtgccacagt ccggcagtca gccacaccat catcacgccg
     8821 cccatcatgc cgccgagggt aatcaggtgc aggggcgcgg aggcaggcag gttttgcaat
     8881 ttcgccgcgc ctgtccacaa atagcctgcg gcggcaaaaa gttggagcag gtaataagtg
     8941 cggacgtagt gtttgcgtaa gagttcgtga tggtgcagtt cgcgcagctt ggcgagcaag
     9001 atgaagccga cggcgagcgc ggtaaaaccg gcggtttgcg cgggcagcca aagttcggcg
     9061 gcggcgtgca gcagcaggaa ggtgatggcg atgtttttat agacaacgtt ggggatgaat
     9121 acagggtctt tcagacggca ttttttgagg gcttccgcgc ccagaaggac actgacgcgg
     9181 acggatacga acatgaccgc cgccatattc aaatgcactt gcgcgcgcaa caggttcaaa
     9241 tcgccggttg cagcgtatgc cgtctgaaaa acagtgaacg cggcaagcag cattaagagg
     9301 gcgaagttgt cggtgtttcg gtctagccaa atcagccggg cgcagaacag cagcaacacc
     9361 agccaatagg cggcgacgaa aaacgaggca gtttgcggcg aaaagggcag tatagcggat
     9421 gcggcgagca ataatgccgc catcaaagtc gcgacaggtt tcaggttacc cgaaaaaacc
     9481 cgtccagtcc aacaaagccg cagtcaggaa accaccatat gctgcaggca gcatgagttc
     9541 taagaagatt tggcggtgca ggacgacggc accggggctg atgaaaaaca ccaacgcgcc
     9601 gagtatggca agcactgtcg cgccgacgaa aaacgggcgc
//
LOCUS       DAWGJV010000072         9625 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWGJV010000072 DAWGJV010000000
VERSION     DAWGJV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.072"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(61..960)
                     /locus_tag="WHQ94_001253"
     CDS             complement(61..960)
                     /locus_tag="WHQ94_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2128525.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(1526..2458)
                     /gene="cysK"
                     /locus_tag="WHQ94_001254"
     CDS             complement(1526..2458)
                     /gene="cysK"
                     /locus_tag="WHQ94_001254"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ2128526.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(2652..3257)
                     /locus_tag="WHQ94_001255"
     CDS             complement(2652..3257)
                     /locus_tag="WHQ94_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128527.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(3254..4105)
                     /gene="dapF"
                     /locus_tag="WHQ94_001256"
     CDS             complement(3254..4105)
                     /gene="dapF"
                     /locus_tag="WHQ94_001256"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ2128528.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            4242..4538
                     /locus_tag="WHQ94_001257"
     CDS             4242..4538
                     /locus_tag="WHQ94_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128529.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            4552..5220
                     /locus_tag="WHQ94_001258"
     CDS             4552..5220
                     /locus_tag="WHQ94_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2128530.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(5293..7413)
                     /gene="pnp"
                     /locus_tag="WHQ94_001259"
     CDS             complement(5293..7413)
                     /gene="pnp"
                     /locus_tag="WHQ94_001259"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ2128531.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(7650..8513)
                     /locus_tag="WHQ94_001260"
     CDS             complement(7650..8513)
                     /locus_tag="WHQ94_001260"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ2128532.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            8712..9575
                     /gene="rfbD"
                     /locus_tag="WHQ94_001261"
     CDS             8712..9575
                     /gene="rfbD"
                     /locus_tag="WHQ94_001261"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ2128533.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2356 a         2794 c         2225 g         2250 t
ORIGIN      
        1 cagtccggta acgcaggttt tgcacggaaa actgccaata ctgccttgat ggaatcaata
       61 ttaagactgc tgtttcagcg aaccgaggta aattgccgaa agcgtcagcg ccacccctgc
      121 ccactgcacg ccttcaatcg gtttgcccaa tccgaaataa tcgatgaaca aggcagcgac
      181 cggttcggac aaaagcagca gccccgtcag cgacagcgaa agcagcggaa tggcataggc
      241 aaccatcgcc cacgcaaaac actgcattac cacgccgtac acaagcacca aacccgcatc
      301 tttccaagtc gtcggataaa gcgcgccgcc atccatcagc aatgccggaa caaccagcga
      361 taccgcgccg cccaaactca aaatcatcat tgaagggaaa agcgccaccg gctcgatttc
      421 atgggttttg cggacaaaca ccatcgacag ggcgagcatc agtcccgata ccaaaccgct
      481 ggcgaatccc caaaccgcat taccgttgta gccgaattcc gcaccggcaa tcatcgccac
      541 gccggcaact gccgatatta agcctgcctt tttcagcccg ctcaaacgct cgccgaaaaa
      601 gaaaacaccg attgccgaca agaaaaagat ttgcaggctg ttgagcaggg tggaaatacc
      661 cggcccgacc gcgtgtatgc tttcgtgcca caacgccaaa tcgaaagcga gaaacacgcc
      721 cgccgtcagg gcatatcgga cggttttcct gttttttggg aatttttgcc cgaaaaaccg
      781 tgctaaaaac caaaatacga acaccgaaat cagcaaccgc caaaatgcga ttgcatacga
      841 accgacgggg acggatctga caatcagact gcccagaccg aacaccacgc aacccagcac
      901 cagcaatggt gcggcatatt ttttgccgtt accgaccaca atatcccctc ccttccccat
      961 acggctgccg cgccgccggc aagaatgccg ccattccgaa aatatgccgt ctgaagccca
     1021 aagccccggc tgcacccgga caaacggctg attttacggg cagaccgact attttccgtt
     1081 ggggcaaaaa accgtcctgt cggataaaac ccgaatatag tggattaaca aaaaccagta
     1141 cggtgttggc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg
     1201 aatcggttcc gtactatagt ggattaacaa aaaccagtac agcgttgcct cgccttgccg
     1261 tactatctgt actgtctgcg gcttcattgc cttgtcctga tttttgttaa tccactatat
     1321 ttgtactgtc tgcggcttcg ttgccttgtc ctgatttttg ttaatccact ataaaaaata
     1381 tttccctcaa gcgatgcagc gacaccaaca cctgattaaa acacaaccgg cgttttcggc
     1441 atatacgcaa aaacgcattt tccggcacgg cgttacaccg ataccgaaaa atgcgtctga
     1501 atgtttcgac aatccgatta aagcattatg ccaaatctgc aaaaagtggc gtagagagat
     1561 agcgttcgcc ataagaaggc agcagcacga ctatcagctt gccttcgttt tcaggctgtt
     1621 tggcaagctg caacgcactc caaaccgccg caccggaaga aatacccacc aaaatgcctt
     1681 ctttttccgc tattgcgcgg gcggtttcaa aagccgcttc gttcggcact ttggtaatgc
     1741 tgtcgtaaat tttggtattc aaaacggtcg gaataaagcc tgcgccgatg ccttgaatcg
     1801 ggtgcgggcc tttttcgccg ccgctcaata cgggtgaagc ttcaggctcg acggcaacca
     1861 ctttaacttc gggtttgtat tttttcaaca cttcgcccac gccggtaatc gtaccgcccg
     1921 tgccgacgcc ggcaacgaag acatcgactt tgccgtccgt atcccgccaa atttcctcgg
     1981 cggttgtttt gcggtggact tcggggtttg cctcattgtc gaactggcgc ggcataaaat
     2041 aagtgtcggg atgcgcgtcc accaaggatt tcgctttggc aatcgcgccc gccatacctt
     2101 cggcggcagg ggtcagaatc agctccgcac caaacgcgcg caacagcatt ttgcgctcct
     2161 tactcatgct ttccggcatg gtaatcgcca gcttgtagcc gcgtgcggca cataccattg
     2221 ccaaaccgac acccgtattg ccgctggttg cttcgacaat gacggtgttt ttgttgattt
     2281 tgcccgcttt ttcggcaccc tcgatcattg cttcggcaat gcggtctttg acgctgctgc
     2341 ctggattgaa aaattccagt ttcacggcaa cctccgcctt caaaccttcg gtcagacggt
     2401 tcagtttgac caaaggcgtg ttgccgatta gttcggtgat gctgtttgca attttcatct
     2461 tctgctcctg aataataagg tttcgacagt aaggcgcaat ccccataggc atcctgtccg
     2521 attgcagggg atttgcgccg aaacataaac aaatttcgga tatggcttta tcctaaacct
     2581 atgccgccta aaaaaccaat atccaaaatt tctttcttta taactttttg ttccgatacg
     2641 gccgcaccgt attatcttcc aatttctgaa agcagcagca ccagtaccga gacctgcatc
     2701 aacgctgccg tcaacccgcc gagtcgcagc tgcgagccgc cgaacgttga cggcggctgc
     2761 cggtctatac cgcagcggtg gacataggca accgccatca gcacaaactg caacacgaac
     2821 caataaaccg gcttcagcgc ataaagtttg agcagtgccg caaacacaaa cacttgaacc
     2881 gttcccgaaa agcaataatg caccaacgca catattccga caaaagcaag cgtcatcgat
     2941 acattactga cggcaaacag cccgagcaga ggatgttcgg ggtctgcctg ccatccgttt
     3001 ccatccgttt tccggttcca atgcccgatg aaaaaaaata tgctgcccaa agtcaggtaa
     3061 aaatggggga tgaacaaggg cgcggcgcgg gtcattcccc atatgccggg catcagcttt
     3121 gcccccaaaa ccgatatgcc cagccacagc ataatactcg cccaaaacca ttgcaacatt
     3181 cctgcgcgga atatcagaaa cagaaaaacc gccgcataca gtaaggcaga caaaaaatcc
     3241 aaatgcagca aaatcatgaa tacgccaact cgccttcaaa caccgcttcc gcagggccgg
     3301 tcatcatcac atcgccgccg caggcccatt cgatatataa agtcccgccc ggcaaaatca
     3361 cctctaccgt tttcccttca tccaacaacc ccagacggat acccgccacc acagccgcac
     3421 acgcgcccgt accgcacgct tgggtttcgc ccacgccgcg ctcgaacacg cgcaaacgaa
     3481 tcgcggttcg gctgacaatc tgcataaagc cgacgttgac gcgttcggga aactgcctgt
     3541 gcggttcgat aagcgaaccg gtttcgcgca ccggcgcgca ttctacgtca tcgaccacaa
     3601 tcaccgcatg ggggttgccc atattgacgc agctgacagg ctgaatgccg gattcgagat
     3661 gcaccccgta aatacaggca tcatccccct cgcccgattc ggggacaaac ggtatttcag
     3721 acggcataaa cctcggtttg cccatattga ccgtaaccat gccgttatcg gacaatttcg
     3781 gaaaaataac gccatttgcc gtttcaacac aaatttcttt cttatcggtc aaacccttgt
     3841 ctgcaacaaa acgggcaaaa caacgcgctc cgttgccgca ttgctcgacc tcgctaccgt
     3901 cggcattgaa aatacggtaa cggaaatcca cgccttcggt ttccgaacgc ccgaccacca
     3961 aaagctggtc gaagcccacg ccccggaagc ggtccgccca tgccgcaatc ggcgcgtcct
     4021 cgggggtaaa atcctgactg accgcgtcaa tcaccataaa atcgttgccc aaaccgtgca
     4081 ttttggtaaa tttcaaagtg ttcatattgt tccgaaatcc cgtatcgtct gttgcttccg
     4141 aatccgaaat cccccgcacc gaagggaaat gcttccatct cccgccgcct gaggattaaa
     4201 atcagccatt atactgtttg ttacccctta caagaaacac catgttccgc atattactgt
     4261 tgcttttccc ttgtgcaatc cttgccgcct gcgcccgaaa cataccggca agccccgccg
     4321 tttcaaattg cgcgcatctt caaaaccgca ccatcctgta ttttgacgca cacggcaaac
     4381 ccgccgacgg cgcagtaaag ggcggatttt acaaaattat cgaaatacag ggtgcaaact
     4441 gcatccttat tcaagacttt aacagcacgg gcaatgccct cggcggatcc tatgtgccgg
     4501 actgccgcaa atcatccctt ttcccgaaaa atatttgact tcaaactgcc tatgaaccac
     4561 gacatcactt tcctcaccct gttcctactc ggtttcttcg gcggaacgca ctgcatcggt
     4621 atgtgcggcg gattaagcag cgcgtttgcg ctccaactcc ccccgcatat caaccgattt
     4681 tggctgatcc tgctgcttaa cacaggacgg gtaagcagct atacggcaat cggcctgata
     4741 ctcggattaa tcggacaggt cggcgtttca ctcgaccaaa cccgcgtcct gcagaatatt
     4801 ttatacacgg ccgccaacct cctgctgctc tttttgggct tatacttgag cggtatttct
     4861 tccttggcgg caaaaatcga gaaaatcggc aaaccgatat ggcggaacct gaacccgata
     4921 ctcaaccggc tgttacccat aaaatccata cccgcctgcc ttgcggtcgg aatattatgg
     4981 ggctggctgc cgtgcggact ggtttacagc gcgtcgcttt acgcgctggg aagcggtagt
     5041 gcggcaacgg gcgggttata tatgcttgcc tttgcactgg gtacgctgcc caatcttttg
     5101 gcaatcggca ttttttccct gcaactgaaa aaaatcatgc aaaaccgata tatccgcctg
     5161 tgtacgggat tatccgtatc attatgggca ttatggaaac ttgccgtcct gtggctgtaa
     5221 gccccattca ttcccgatac ccgtaaaaaa taccgtctga aacctgttca gacggcactt
     5281 tcaccctaag cgttactcgg cggcattttc ctcacgggca ggcgcgtcca gcagggcttt
     5341 gatggacaga cggacacggc ctctgtcgtc cacttccaat gctttcacgt tcaccacctg
     5401 accgacttgc aggtagtcgc cgacattgcg tacgcgctcg tgggcgattt ggctgatgtg
     5461 taccaaaccg tctttgcccg gcatcacgct gacaatcgcg ccgacgttgt tgtcgaggat
     5521 tttcaccaca gtgccttcat acactttgcc cacttccact tcggcagtaa tctgctcgat
     5581 gcgttttttc gccgcatcgc cggcttcttg agtggtcgcg gcaatggtaa tcgtaccgtc
     5641 ttcggcaata ttgatttccg taccggtttc agcggtaatc gcacggatgg tttcaccgcc
     5701 cttaccgata acttcgcgga ttttgtcttg gttgattttc atcgtaaaca aacgcggcgc
     5761 gtgtgcggac aactcttgcg ggcccgcaac ggcggctttc atctgatcca agatgtgcag
     5821 acgcgcttct ttggcctgtg ccaaagcgat ttgcataatt tctttggtaa tgccttggat
     5881 tttgatgtcc atttgcagcg cggtaacgcc ttcggtcgta ccggccactt taaagtccat
     5941 atcgcccaag tggtcttcgt cgcccaaaat gtcggtcagg acggcaaatt tgttgccttc
     6001 cagaatcaga cccatcgcga taccggcaac gtgtgctttc aaaggcacgc cggcagacag
     6061 caggctcaaa cagccgccgc agacggaagc catagaggaa gagccgttag attcggtaat
     6121 ttcggagacc acgcgcatgg tgtagctgaa atcttcaggt ttcggcaata cggccaacaa
     6181 tgcacgttta gccaaacggc cgtgaccgat ttcacggcgt ttcggcgcgc ccatgcggcc
     6241 cacttcgccg gtagagtacg gcggaaagtt gtagtgcagc ataaagcggt cggtgtattc
     6301 gccggacagc gcgtcgatga tttgctcgtc gcgcgaagta cccaaagtcg ctacggcaag
     6361 ggcttgggtt tcgccacggg taaacaatgc ggaaccgtgc gtgcgcggca atacgccggt
     6421 ttggatgttc agcggacgga cggtgcgggt gtcgcggccg tcgatgcgcg gttggccgtc
     6481 caaaatttgg ctgcggacga catcggcttc caagtgtttg aaaatgcctt tgatttcgtt
     6541 ggctgccagc gtgtcggttt cttcggtaat caaggcttct tttaccgcac tccaagcttc
     6601 gtccaatttg gcagaacgcg cttgtttttg acggattttg aacgcttctt taatggtttc
     6661 gccggcaatc ccgcggactt tggcaaccag ttcctcattg gtttcaggtg ctttccaatc
     6721 ccaaagttcc ggattgactt cgtcggcaaa ttcattgatt gcattgatgg caacctgcat
     6781 ttgatcgtgg ccgtaaacca ccgcgcccag catcacgtct tcgggcagga ttttggcttc
     6841 ggattccacc atcaacacgg ctttggaagt accggcgacc accaagtcca attgcgattt
     6901 cgccaattcg gctttagtcg ggttcaaaac gtacacgccg tttacataac cgacgcgtgc
     6961 cgcgccgatc gggccggcaa acggtacgcc gctcaacacc agcgcggcag acgcgcccaa
     7021 cattgcagga atatcagaat cgatttcagg atcgacggac acgaccatcg ctacgatttg
     7081 gatgtcgtgg tagaaacctt cagggaacag cggacgaatc ggacggtcga tcagacggct
     7141 ggtcaggatt tctttttcgc tttgtttgcc ttcgcgtttg aagaaaccgc cgggaatttt
     7201 gcctgcggcg taagtgcgct cgaggtaatc gacagtcaga gggaagaagt cttggccttc
     7261 tttcacttct ttgttggtgg taacggcaac caaaaccacg gtgtcgccca tagagacttt
     7321 aacggcagcg gcggcttggc gggcaatttc gccggtttcc aaagtaacgg tgtggttgcc
     7381 gtattggaaa gttttaacgt gtttgtcgaa cattattgtt cctttcaaaa taccgcactg
     7441 ctaaaacact aataatgcac actaaaatcc gaatgtgcat agttagggtt tcagaccgtg
     7501 cggcaggttg taaacaagct ttcagacgac atttcggacg ctgaaagcaa ttccaaatta
     7561 taaaggcaac catcattaaa atccagtatt gatacaataa aaaggccgtc tgaaccatat
     7621 ttctttcaga cggccttcaa acttaaaaat caatcctgag tcagcaaagt caatgcttcc
     7681 cgatacttct cgacagtttt ctgaatcaca tcggcaggca ctttcggcgc aggggctttt
     7741 ttgttccaac cgctttgttc cagccagtcg cggacgaatt gtttgtcaaa agacggcgga
     7801 ttggtgccga ctttgtattg gtcggcaggc caaaaacggc tcgaatcggg agtcaatacc
     7861 tcatccatca gcgtcagcgt accgtttgta tccaaaccga attcaaattt ggtatcgcaa
     7921 atgatgatgc cgcgcgattt ggcatattcc gccgcttctg tataaagctg aatcgctttg
     7981 gcgcgcactt ccgccgccaa ttcttttccg ataatgcgtt cgcattcttc aaagctgatg
     8041 ttttcatcgt gatcgccgac tgcggctttg gttgagggcg taaaaatcac ttcaggcagt
     8101 tgttgcgctt cctgcatacc ttcaggcagt tgaataccgc aaaccgagcc ggttttttga
     8161 taatctttcc aaccgctgcc tgccagataa ccacgcacaa tcgcctctac tttcaccgga
     8221 gtgagctttt tagccacgac ggcgcgtttc tctaaagctt tggcttcgtt ttcaggcaaa
     8281 acatcgtaaa ccgtttgacc ggtaaagtgg ttgggcataa tatgcaccag ttttttaaac
     8341 caaaaattgg aaatctgcgt cagaatctcc cctttgctcg gaatcgggtc gtccaaaatc
     8401 acatcgaatg cagacaggcg gtcggaagcg accatcagca tacgtttatc gtcgatttca
     8461 tataaatcgc gcacttttcc aaaatagatc tttaccaaac caatctcact catttcgccc
     8521 cccctgaaaa tatcttgaaa ataccgaccc gacacccgac aggtttgaat cacaaaccaa
     8581 tattctagcc gaagtcggcg caaaacaata cccatggcac aaaaaaccaa cccgtcaacc
     8641 gtcggcaaag ttttggcact ataataccga cagcaagtcc tacaatacac ttttaccaaa
     8701 ggaaatacct catgagaatc ctattgacag gctcgaaaag ccaactggca cgctgcctgc
     8761 gcgaccgtct tccggaagac tgggaaacca ttgcgacgga ttccgcatcc ctagacatta
     8821 ccgatgccga tgccgtctgc aacatggtca aaagtttcca acccgacgcc attgtcaaca
     8881 cggctgccta tactgccgtc gacaaagcgg aaggcaatgc ggcagcggca tttgccgtca
     8941 atgcttccgc cgtttacaac cttgccttgg cagcacatcg cgcccatgcc cgattcatcc
     9001 acatctcaac cgactatatc tttgacggta aagggaaaag gccctatcag gaaagcgact
     9061 ttaccaatcc ttccaatgta tacggacaat ccaaaaccgc aggcgagctg ctcgcactgt
     9121 ctgccaatcc cgacagcctt atcctgcgga cttcttggct gtttagcgaa tacggggaca
     9181 actttgtccg cacgatgctg aaccttgcgc gggaacgttc cccgctgtcc gccgtccaca
     9241 accaaatcgg ctgcccgacc tatgccggcg atttagctgc cgccatcatc cgccttttgc
     9301 agcaaacgtc gcccgtgcgc ggcatttacc actacgccgg cggcaaatcc gtatcctggt
     9361 atgaatttgc ccaacatatt ttccaagcgg catcgcaaca gcagacatcc ttccccgttc
     9421 ccgaattgac tgccgtttca gacaaggaat atccgaccgc cgcccccagg cccgcataca
     9481 gcattttgga ctgccgcaaa atcgaaaacg acttcggcat caaaccgtca gactggcaaa
     9541 aagcccttgc gcaggtcgtt tccaagctgc tctgatgccg cccgcctctc cgtttccgcc
     9601 gtcaagcacc gccttggcgg ttttc
//
LOCUS       DAWGJV010000073         9408 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWGJV010000073 DAWGJV010000000
VERSION     DAWGJV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.073"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(113..1555)
                     /gene="aldA"
                     /locus_tag="WHQ94_001262"
     CDS             complement(113..1555)
                     /gene="aldA"
                     /locus_tag="WHQ94_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ2128534.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1590..1790)
                     /locus_tag="WHQ94_001263"
     CDS             complement(1590..1790)
                     /locus_tag="WHQ94_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128535.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1799..2188)
                     /locus_tag="WHQ94_001264"
     CDS             complement(1799..2188)
                     /locus_tag="WHQ94_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128536.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2396..3313)
                     /locus_tag="WHQ94_001265"
     CDS             complement(2396..3313)
                     /locus_tag="WHQ94_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2128537.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRRRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWNLSPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3485..4285
                     /locus_tag="WHQ94_001266"
     CDS             3485..4285
                     /locus_tag="WHQ94_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128538.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4334..5110
                     /gene="mlaE"
                     /locus_tag="WHQ94_001267"
     CDS             4334..5110
                     /gene="mlaE"
                     /locus_tag="WHQ94_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ2128539.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5161..5655
                     /gene="mlaD"
                     /locus_tag="WHQ94_001268"
     CDS             5161..5655
                     /gene="mlaD"
                     /locus_tag="WHQ94_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ2128540.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5692..6282
                     /locus_tag="WHQ94_001269"
     CDS             5692..6282
                     /locus_tag="WHQ94_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ2128541.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6339..6617
                     /locus_tag="WHQ94_001270"
     CDS             6339..6617
                     /locus_tag="WHQ94_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ2128542.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6614..7441
                     /locus_tag="WHQ94_001271"
     CDS             6614..7441
                     /locus_tag="WHQ94_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ2128543.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7566..7928
                     /locus_tag="WHQ94_001272"
     CDS             7566..7928
                     /locus_tag="WHQ94_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ2128544.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7925..8311
                     /locus_tag="WHQ94_001273"
     CDS             7925..8311
                     /locus_tag="WHQ94_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ2128545.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8308..8796
                     /locus_tag="WHQ94_001274"
     CDS             8308..8796
                     /locus_tag="WHQ94_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ2128546.1"
                     /translation="MKKILTAAVVALIGILLAIVLVPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8877..9044)
                     /locus_tag="WHQ94_001275"
     CDS             complement(8877..9044)
                     /locus_tag="WHQ94_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ2128547.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9011..9385)
                     /locus_tag="WHQ94_001276"
     CDS             complement(9011..9385)
                     /locus_tag="WHQ94_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ2128548.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2156 a         2630 c         2381 g         2241 t
ORIGIN      
        1 agacaaaaac atcgatgtcg gtcatgactg attgccggtg aaactgtaaa aatgccgtct
       61 gaacctgaaa acgggttcag acggcatttt ctatcggggt tttaaagcgg cattaaatgt
      121 cggtttccag ataaacgact tgggtttgca gatattcttc caaaccgtgt ttgccgtccg
      181 cgccgccgat gccggatttt ttccagcccg cgtggaaacc ctgcatggct tcgaagtttt
      241 cgcggttgat gtaggtttcg ccgaattgca ggcggcgggt aacgtagaag gcttcgttta
      301 aattagtcgt ataaacagaa ctggtcagac caaactcgca atcgttagcc agtgcgatga
      361 cttggtcgag cgtgtcgaaa gcggaaacgg gcagcacggg gccgaaggtt tcttctttca
      421 taatgtccat actgttgtcg gtgtcggtca gcagggtcgg ctcgaagaaa tagccgcgcc
      481 cttcggcgcg tttgccgccg cagaccagtt tcgcaccttg tttgactgcc cgttccactt
      541 tttcggcaac ggctttgacg gcgcgctctt caatcagcgg acccatttcc agcgcgcctg
      601 cttcggcttc ggcagggttg ccgtagcgca cgcctttcat ggcggcggtc attttttcaa
      661 tgaacgcgtc tttcaggctg ctgtggacat agacgcgctc ggcgcagttg cagatttgac
      721 cggtgttgcc gacgcgcgaa gccaagatgg atttcaccgc caaatccaaa tccgcgtctt
      781 tcaaaacgat ggcgggggct ttgccgccga gttccagcga aactttggtg atgttggcgg
      841 aagcggcttc catcacttgg cggcctgctt cgacggagcc ggtcaggctg accatatcga
      901 cttgcggatg ggcagacaag gcattgccga tttccgcacc cggaccgttc accacgttga
      961 acacgcctgc gggcaatccg accgcatcga cgatttcggc gaagatgtgg cagttgatcg
     1021 gggttacgct gctgggtttg acaacgatgg tgttgcccgt tactaaggcg ggacccattt
     1081 tgcgggcaat caggaagaaa gggaagttcc acggcaaaat gccggcaatt acgcccagcg
     1141 gacgtttgaa caataaaatg ttttcgcgcg ggcggtcgct ttggatgatt tcgccttcgt
     1201 aacggcgcgc ccattcggcc tggtaatcga gatagtcggc ggtaaacatg acttccacgc
     1261 gtgccaagtc tttggttttg ccaccttcgg caacgatggt gtcggtcagc tcgtcggcac
     1321 gttcgcgtat gccttgggcg attttgcgca aatacgcgcc gcgttcgacc gcaggcagac
     1381 gttcccaagc cggttgcgcc gcgcgtgccg ccgccacggc tcggtcaacg tccgctttgc
     1441 cgcctttggg ttcgcgggcg atggtttctt cggtggacgg gttcaatacg tcgcgccatt
     1501 cgccgttgaa gtcgttttca aagcgtccgt tgatgtacat ggccaattgt ttcatttcgg
     1561 gttctccagt tttgtagtca gatgtagttt tagtttattc ccaaataaat taccgtacaa
     1621 gtttcttttg aaatgtttag acgagaagct actatgtctg catattgttc tgctaattta
     1681 tattcactcg aatgcaactg ccgattattc caatctgcat tcgcaccagt aaaagcccca
     1741 gcattgccgc ctgtggcata accaatcgct aaactgccaa cggtatccac aacaggtttc
     1801 aggctgcctg aatttctcta ataaatgaaa cacttattct atcagccgtt aatgtaacca
     1861 aacaattatc aagataagag tagtttatta aatactcatc ttcttcaaat tgtatatttt
     1921 gtagggttag atgattattt tttaattctg ccgttatttc atacttgaaa ctatcatcta
     1981 tttttctaaa tccaatattt gaagttgatt tatccagtat gtaatcatta aagatttcat
     2041 aggataattc cacttcataa aattgatttt caaatatttt gaatgggcag tacgatataa
     2101 aacaatgtag ttcataacca tttatttcaa tcaatactgc ctcttcaatg gtgttatcta
     2161 actttttgat tttacattta tataacattt atcttgcctt tggataaatt gttttgtttt
     2221 ttggattgtt attcaaagtt ctccaaaagt cttttcctga ttttgtatgt atcgtggtaa
     2281 tactaccatt ttcattggtt ccaacaaaaa taaatttaga ttgacctttg gaatcagtac
     2341 ccaaaaactg aacatatcca gacatgcgga tttcgggttt taaatcaaat gcggcttacc
     2401 ggttttcttt tttggtttcc gcaggcgtgc tgccgaaata ttgcctgaat gcctgagtaa
     2461 agctggaaac gtggcggtag ccgcacaaat acgcggtttc gcctatgctt ttgcccccgt
     2521 tttgcaacag gtagagcgcg tgctgcattt gtttgtggtg cagccactcg cttgcggtaa
     2581 taccgaaatg gtcgcgcata cggcgttgca gcgtcctttc actgatgttc agcgcgtctg
     2641 tcagccggtt gacttggtgt gcgccgtcgg caaacgcggc atttagggtg cggttgaagt
     2701 cttcagacgg catagcgtct gcttccgccg tttgccccgc cgccggcccg atgccgtctg
     2761 aaaccgtgtc ccacaagtcc gacagcagcc gcaaaacgtc cgcctcgcgg cgcaatgttt
     2821 cgcccaaatg cccctttggg acagctttca ggcaggatgc cgccaagccg cgcaggtttg
     2881 gggacaaatt ccatattctg accggttcgc ggtaaagcag gggtgcgaaa cgcgcatact
     2941 ccggacgcag cagccattgt tccatacctt taatggtcat tttgaccgtt ttgccgcctc
     3001 ggtaaagata gcggctgaac aggacctctt ccccgacagc aattaggacg atcttgccgc
     3061 catccgcatc gatttggaag cggcggcggt tgatgccgaa gtccaaactg ccttccagca
     3121 agagcacgaa cgacacataa ggttcggcga ggcggctgct gcaaaaatcg caccgtgccg
     3181 ttaccgtgcc gccgtggatg gaaatgccgt tggacaaagt atcgaaacgg tagctgccct
     3241 ccacatagct gccgccgccg cttttgacca gattgacgaa ccggctgctg ttcaaatgtt
     3301 ctgctttttc catagttttc tgataaaggt tatcatttga aagataacat ttttcgccac
     3361 agcaaacaat ccgccgccac aacggcattt tcacaacatt taccgtccgt atgccgtctg
     3421 aaaattgccc gtcttcccga taagccttaa aatacacgcc gtcaaaccta ccgtccgtcc
     3481 gtacatgagc ccatccccct ttatcgaaat gaaagacgtc gccttcgcgt atggcgaccg
     3541 cccgattctg aagaacatca atttcagcat tccgcaaggc aattttgctg ccgtaatggg
     3601 cggttcgggc agcggcaaaa ccacgctgat gcgactgatt acaggacaga ttcgtccgca
     3661 gtccgggcag gttttgattg aaggacggga tttggcgggc ttttcggctg acgagttgta
     3721 tgaacaccgc cgccgtatgg gcgtattgtt ccaacacggc gcgctgttta ccgatttgtc
     3781 tgtatttgac aatattgctt ttccgatgcg cgaactgacg cgcctgccgg aagcggtgat
     3841 tcgagatttg gttttgttga aactgaacgc ggtcgggctg cgcggcgtgg aaaacctgat
     3901 gccgtccgag ttgtccggcg ggatgtcgcg ccgcgtcgcg cttgcccgca caatcgcgct
     3961 cgatcccgaa attatgttgt acgacgagcc gtttaccggt ctcgatccga tttccttggg
     4021 cgtgattgcc cacttgatca gccgcgtcaa caaggctttg cgttcgacca gtattatggt
     4081 cacgcacgac attgaaaaat ctttggaaat cgtcgatcag gtgattttct tggcgcacgg
     4141 cgaaattatg ttctccggct cgccgcagga aatgcgcgaa ctggattcgc cttgggtgcg
     4201 ccagtttgtc ggcgggctgg cagacggccc cgtggcatac cgttatccgg cgcaaacgtc
     4261 gttgcagcag gatttgctcg ggtaaattga gccggtgccg tctgaaaccg ttgaacttta
     4321 catatagaaa tttatgaatt ttatccgttc cgtcggggcg aaaaccctcg gccttattca
     4381 atccctcggc agtattacgc tgtttctgct gaacattctg gcgaagtccg gtacggcttt
     4441 cgtccgtccg cgcctgagcg tgcgccaagt gtattttgcc ggcgtgctgt cggtgctgat
     4501 tgttgccgtt tcggggctgt tcgtcggcat ggtcttgggt ttgcagggct atacgcagtt
     4561 gtcgaaattc aaatccgccg atattttggg ctatatggtt gcggcttcgc tgttgcgcga
     4621 actgggtccg gtgttggcgg cgattctgtt tgccagcagc gcgggcggtg cgatgaccag
     4681 cgaaatcggt ttgatgaaaa cgaccgaaca gctcgaagcg atgaacgtga tggcggtaaa
     4741 ccccgtcgcc cgagtggttg cgccgcgctt ttgggcgggc gtgttttcca tgccgctttt
     4801 ggcttcgatt ttcaacgtgg cgggtatttt cggcgcgtat ttggtcggtg taacctggct
     4861 gggcttggac agcggtattt tctggtcgca aatgcagaac aatatcacga tacattacga
     4921 tgtaatcaac ggtctgatta aatccgccgc gttcggcgtg gcggtaacgc tgattgccgt
     4981 gcatcagggc ttccattgcg tcccgacttc ggaaggcatt ttgcgcgcca gcacgcgcac
     5041 ggtggtttcg tccgccctga cgattttggc ggtcgatttt atattgaccg cgtggatgtt
     5101 tacagattga caggccgtct gaagacgaaa cgcggaacat cggatattca aggaacttta
     5161 atgaaaaaga acatattgga attttgggtc ggactgttcg tcctgattgg cgcggcggcg
     5221 gttgcctttc tcgctttccg cgtggccggc ggtgcggcgt tcggcggttc ggacaaaacc
     5281 tacgccgttt atgccgattt cggcgacatc ggcggtttga aggtcaatgc ccccgtcaaa
     5341 tccgcaggcg tattggtcgg gcgcgtcggc gctatcggac ttgacccgaa atcctatcag
     5401 gcgagggtgc gcctcgattt ggacggcaag tatcagttca gcagcgacgt ttccgcgcaa
     5461 atcctgactt cgggactttt gggcgagcag tacatcgggc tgcagcaggg cggcgacacg
     5521 gaaaaccttg ctgccggcga caccatctcc gtaaccagtt ctgcaatggt tctggaaaac
     5581 cttatcggca aattcatgac gagttttgcc gagaaaaatg ccgacggcgg caatgcggaa
     5641 aaagccgccg aataaacgta tgccatcata ttcaaactag gacacttaat catgaaaaaa
     5701 tcctccctca tcagcgcatt gggcatcggt attttgagca tcggcatggc atttgccgct
     5761 cctgccgacg cggtaaacca aatccgtcaa aacgccactc aagtattgag catcttaaaa
     5821 agcggtgatg ccaacaccgc ccgccaaaaa gccgaagcct atgcgattcc ctatttcgat
     5881 ttccaacgta tgaccgcatt ggcggtcggc aacccttggc gcaccgcgtc cgacgcgcaa
     5941 aaacaagcgt tggccaaaga atttcaaacc ctgctgatcc gcacctattc cggcacgatg
     6001 ctgaaattaa aaaacgccaa cgtcaacgtc aaagacaatc ccatcgtcaa taaaggcggc
     6061 aaagaaatca tcgtccgcgc cgaagtcggc gtacccgggc aaaaacccgt caacatggac
     6121 ttcaccacct accaaagcgg tggtaaatac cgtacctaca acgtcgccat cgaaggcgcg
     6181 agcctggtta ccgtgtaccg caaccaattc ggcgaaatca tcaaagcgaa aggcgtggac
     6241 ggactgattg ccgagttgaa ggctaaaaac ggcagcaagt aatccataag ccatgccgcc
     6301 agactgaaga tggcatggat tttttcctac cgaacactat gcatacagaa ctcaacaacg
     6361 gaacactgca catcagcggc gacgtaaccg tcaaaaccct gaccgccgcc gcctttaccc
     6421 gcttcagaca gcaatgtcgt ctgaaagaca cccgcgcggt tgatttgagc ggcgtcggac
     6481 gcgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct gcgcggttgc aaaggcagcg
     6541 tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc cgaactgtac gaaatcaaag
     6601 actggctgaa atcatgaaaa aaaccgccta tgccttcctc ctgctgatcg ggttcgcttc
     6661 cgcccctgca tttgccgaaa cccgccccgc cgacccttat gaaggctaca accgcgccgt
     6721 tttcaaattc aacgaccaag ccgaccgcta cattttcgcc cctgccgcgc gcggctaccg
     6781 caaagttgcg ccgaaacccg tccgcgccgg cgtgtccaat ttttttaaca acctgtgcga
     6841 cgtggtcagc ttcggcagca atatcttgcg cttggacatc aaacgcgcaa gcgaagacct
     6901 tgtccgcgtc ggcatcaaca ccactttcgg tttgggcggg cttatcgaca tcgccggcgc
     6961 gggcggcatt cccgacaata aaaacacctt gggcgacacg tttgcttcgt ggggatggaa
     7021 aaacagcaat tatttcgtgt tgcccgtctt agggccgtcc accgtccgcg acgcgctcgg
     7081 cacgggtatt acctccgttt attcgcccaa gaatatcgtc ttccgcaccc ctgtcggacg
     7141 ctggggcacg actgccgtat ccgccgtcag tacgcgcgaa ggtctgctcg atttgaccga
     7201 cagtctggac gaagccgcca tcgacaaata cagctacacg cgcgacctct atatgaaagt
     7261 ccgtgcgcgg cagaccggtg caacacctgc cgaaggtacg gaagataaca tcgacatcga
     7321 cgaattggtc gaaagtgccg aaaccggcgc ggcggaaact gccgttcaag aagattccgt
     7381 atccgaaaca caggcagaag cagcagggga agccgaaacg caacctggaa cacaacctta
     7441 aagccatgcc gtctgaaaat ccgtgttcgg acggcattcc cttctgtcgg aacggacagt
     7501 ccgaaaccac agattgggct ataatctctt ttttgcgcat accgcgcgac agcaaaggaa
     7561 aaaccatgta cgaagtcaac cgcagcgtat tcgtcctcat ccctttagaa cctttttgga
     7621 actggctgca aaccctgccg ggcaaccacc tcgacggact gactcttgaa gacatccaag
     7681 ccgacgccaa ttcctacctt gtccgcccgt gcgaaaccgc cgacgaagta tgggacgaaa
     7741 tcgaagcccg ttttgaagac attttcgccg ccgaacttgc cgactggtgc gaagacgaac
     7801 gcgagtggcc tgcgctcgat gccgacattt tcaacgaatg gttcgacatc cagctttcta
     7861 ccgtcatcac cgacctcgaa cacgaaccgc ttgcccgcga agccttccaa cccatcaacc
     7921 tgaactgatg aagctgaccg tccgcaacta ccatctcgac ggctacggcc atgtcaacaa
     7981 tgcgcgctac ctcgaatttt tcgaagaagc gcgctgggcg ttttttgaag aacgcggact
     8041 gctgcacgag ttggcaggcc tcatactgat tgtcgcccgc atcgacatcc gatacagccg
     8101 ccccgccgtc gaaggcgacg tattgcagtt ttcatgccgc ctgaaaaccc ccggcacgcg
     8161 ccgtattgtg ctgacccaga cgattacact gccaaacggc aaaaccgccg cagaagccga
     8221 catcaccctg atgcccgtcc acgccgccac acaacgcacc gtcagcctgc ccgccaccct
     8281 tgcccgcgca ttggaagcct tgtccgaatg aaaaaaatac tcaccgccgc cgtcgtcgca
     8341 ctgatcggca tcctccttgc catcgtcctc gtccccgaca gcaaaaccgc gcccgccttc
     8401 tccctgcccg acctgcacgg aaaaaccgtt tccaacgccg acctgcaagg caaagtaacc
     8461 ctgattaatt tttggtttcc ctcctgtccg ggttgtgtga gcgaaatgcc caaaatcatt
     8521 aaaacggcaa atgactataa aaacaaaaac ttccaagtcc ttgccgtcgc ccagcccatc
     8581 gatccgatag aaagcgtccg ccaatatgtc aaagactacg gtttgccgtt taccgtcatg
     8641 tatgatgcgg acaaagctgt cggacaggcg ttcggcacac aggtttatcc gacttccgtc
     8701 cttatcggca aaaaaggcga aatcctcaaa acttatgtcg gcgaacccga tttcggcaaa
     8761 ctctaccaag aaatcgatac cgcgctggcg caatagcgat gccgtctgaa gcgcaaaaaa
     8821 cagaaacgat gttcctgttt tttcagatca tccgccccga cacgttcaga cggcattcaa
     8881 ccttccggca gattcttttt caccactgcc ggattgcctg ccgccaaaga gtagggcgga
     8941 atgtcttttg taaccaccgc gcctgcgccc acgaccgaac cgcgtccgac ggttacgccc
     9001 gccattacaa tcaccctgcg ccccggccag acatcgtcct ccaacgtaat cggacggatt
     9061 tccgtgtagc cctcaaagcg ttttttgaac ggtcaaactt gtggttattt gaataaaaca
     9121 gacattccgg ctccatcatc acatttttgc cgaccaccgg cccacggcag atttcacagt
     9181 ttgccccgat gcccgagccg tcgcccaaaa ccgtatccgg aaacacatac gccccgcgtt
     9241 cgatattgac cccgcgtcca atatgcggag aaacccgccg cgccaaaaaa ccgcgtaccc
     9301 gtctgccgac gatgccgcgc atatgcgacg gcggcaacac cgaaccggca aaacgcagca
     9361 gcaggaaaaa gaattttgat cccatcaaac ctccctcaaa agaacttt
//
LOCUS       DAWGJV010000074         9379 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWGJV010000074 DAWGJV010000000
VERSION     DAWGJV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.074"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            192..1010
                     /gene="opc"
                     /locus_tag="WHQ94_001277"
                     /gene_synonym="opcA"
     CDS             192..1010
                     /gene="opc"
                     /locus_tag="WHQ94_001277"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ2128549.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADILFDASQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1173..1817
                     /locus_tag="WHQ94_001278"
     CDS             1173..1817
                     /locus_tag="WHQ94_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2128550.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1836..3748
                     /locus_tag="WHQ94_001279"
                     /pseudo
     CDS             1836..3748
                     /locus_tag="WHQ94_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3769..4134
                     /locus_tag="WHQ94_001280"
     CDS             3769..4134
                     /locus_tag="WHQ94_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128551.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4245..5720
                     /locus_tag="WHQ94_001281"
     CDS             4245..5720
                     /locus_tag="WHQ94_001281"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ2128552.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5717..6263
                     /locus_tag="WHQ94_001282"
                     /pseudo
     CDS             5717..6263
                     /locus_tag="WHQ94_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            6260..7396
                     /locus_tag="WHQ94_001283"
     CDS             6260..7396
                     /locus_tag="WHQ94_001283"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ2128553.1"
                     /translation="MKNISLSPPATLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFNDQTALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7443..7925
                     /locus_tag="WHQ94_001284"
     CDS             7443..7925
                     /locus_tag="WHQ94_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2128554.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8031..9086
                     /locus_tag="WHQ94_001285"
     CDS             8031..9086
                     /locus_tag="WHQ94_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2128555.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9083..9280
                     /locus_tag="WHQ94_001286"
     CDS             9083..9280
                     /locus_tag="WHQ94_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128556.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
BASE COUNT         2614 a         2673 c         2062 g         2030 t
ORIGIN      
        1 tcgtcattcc cataaaaaac agaaaaccaa gtgagaataa caattcgttg taaacaaaca
       61 actatttgtt aatttttatt aatatatata aaatcccccc ccgaaagctt aagaatataa
      121 ttgtaagcgt aacgattatt tacgttatgt taccatatcc gactacaatc caaattttgg
      181 agattttaac tatgaaaaaa acagttttta catgtgccat gattgccctg accggtactg
      241 ccgccgctgc acaagagctt caaaccgcta atgagtttac cgtccacacc gacctctctt
      301 ccatttcttc aactcgtgct ttcctgaaag aaaaacacaa agctgccaaa cacatcggcg
      361 tacgtgctga tattcttttt gatgccagcc aaggcatccg cttggaagcc ggtttcgggc
      421 gcagcaaaaa aaatattatt aatttggaaa cagatgagaa caagctgggt aagactaaaa
      481 atgtaaaact acccaccggc gttcctgaaa accgtatcga tctttacaca ggctacacct
      541 atacccaaac gttaagtgat tctttaaatt tccgtgtggg tgccggcttg ggttttgaat
      601 cttcaaaaga cagcattaaa accaccaagc atacgcttca cagcagccgt cagtcgtggt
      661 cagccaaagt tcacgcggat ttgctttccc aactgggtaa cggctggtat atcaaccctt
      721 ggtctgaagt gaaatttgac ctcaattccc gctataaatt aaacaccggc gttaccagtc
      781 tcaaaaaaga catcaatcaa aaaaccaacg gctggggctt tggattgggt gcaaatattg
      841 gtaaaaaact gggcgaatcc gccagcatcg aggcggggcc gttctacaaa caacgcactt
      901 acaaagaatc cggcgagttt agtgtaacaa ccaagagtgg cgacgtatcg ctcaccatcc
      961 cgaaaaccag tattcgtgaa tacggcttgc gcgtcggcat aaaattctga tgatttgaaa
     1021 tcatcattgt cactttaaat gccaaaccgc aaactatttt ggtttgcggt ttttacgtga
     1081 aatgaatttg aatagccgat gccgtctgaa aatttcagac ggcatttttc caatcggtta
     1141 aaatacgcgg tttgtttttt taggaacgcg ccgtgcttgc ctccgccatc gacttcatcc
     1201 tccatatcga ccaacacctg ctcgcgctgt cggcgcaata cggtgtgtgg atttatgcga
     1261 ttctgttttt gattgttttt tgcgaaaccg gcctgattgt tacgccctta ctgcccggcg
     1321 attcgctgct gtttgcggcc ggcggtattg ccgcgctggg cggtatggat attcatttga
     1381 tggttgcatt attaagcctt gccgccatac tcggcgacgc gctcaatttt accgtcggca
     1441 aatatttcgg cggcaggctg ttcgccaatc ccgattctaa aatcttccga cgtgaatatc
     1501 tcgataaaac ccaccgtttt tacgaaaaac acggcggcaa aaccatcatc attgcccgtt
     1561 ttatgcccat cgtccgcacc ttcgccccct tcgtcgccgg tatggggaaa atgcactatg
     1621 ccaaatttat ccgctacaac atcatcggtg gcttgttgtg ggttatcctg ttttcttacg
     1681 cgggctattt ctttgccaac ttccccgtcg taaaaaacaa tctgggcttg gtgatgggcg
     1741 gcatcattat tgtttccgtc ctgccgggtg cggttgaaat tgcccgcgcc aaacttgcgg
     1801 taaaatccaa acggtaactg ttgagaattt gtatcatgaa tatattatct gtagaaaacg
     1861 cttcctttgc cgtcggccac gtcgccctgc tcgacaaaac ttccttccaa ctcgacagcg
     1921 gcgaaaaaat cggcttaatc ggtcgtaatg gtgcgggtaa gtcttcgttt ttaaaaatcc
     1981 tcgccggcgt gcaaaagctt gatgatgggc agattattgt ccaaaacaac ctcaaaatcg
     2041 tttatgtacc gcaggaatcc ttttttgata aggaagcaac cgtatttgat actgttgccg
     2101 aaggtttggg cgaaattcgc gatttattgc gccgttatca tcatgtcagc catgagttgg
     2161 aaaatggttc gagtgaggcc ttattgaaag agctcaacga attgcaactt gagatcgaag
     2221 cgaaggacgg ctggaagttg gatgcggcgg tgaagcagac tttgggcgaa ctcggtttgc
     2281 cggaaaacga aaaaatcggc aacctctccg gcggtcagaa aaagcgcgtc gccttggcgc
     2341 aggcttgggt gcagaagccc gacgtattgc tgctcgatga accgaccaac catttggaca
     2401 tcgacgcgat tatttggttg gaaaacctgc tcaaagcgtt tgaaggcagc ctggttgtga
     2461 ttacccacga ccgccgtttt ttggacaata tcgccacgcg catcgtcgaa ctcgaccgag
     2521 gtattttgcg ttcctatccc ggctcgttct ccaaatacag cgaaaaaaaa gtgcaggaat
     2581 tggcggttga agcggaacac aaccgcctgt ttgacaaatt ccacgcgcag gaagaagcgt
     2641 ggatacgcaa aggcatcgaa gcgcgccgta cccgcaacga aggccgcgtg cgccgtttgg
     2701 aagaactgcg ccgccagcgt gccgaacgcc gcaacgtaca agggcaggtc aatttcaagc
     2761 tcgacagcgg cgagaaaagc ggcaaaatca tcgccgagct ggaacacgcc tcgtttgctt
     2821 atggcgacaa agtcattatg gacaaattct ccgccatctt gcagcgcggc gacaaaatcg
     2881 gcttaatcgg tccaaacggc atcggcaaaa ccacattttt aaaactgatt ttgggcgaat
     2941 tgcagccgac ctacggcaga atccgtatcg gcagcaagca ggaagtcgcc tatttcgacc
     3001 agttccgcag cgcgttgaat gaaaacgaca ccgtgtttta cacgctcgga cagggcaatg
     3061 attacgttga agtcggcggt aagaaaaagc atgtgatgag ctatttggaa gatttcctgt
     3121 tcccccccct gcccgcgctc aaagtcctgt ttcttcactc tccggcggcg aacgcaaccg
     3181 ccttttgctg gcaaaactct ttacccgtcc cgccaatatt ttggtcttgg acgaaccgac
     3241 caatgacttg gacatcgaca cccaagagct gctcgaagac ctgttgcgcg attatcaagg
     3301 cacggtattc cttgtctcgc acgaccgtat gttcctcgat aacgtgatta cccaaagcct
     3361 tgtttttgaa ggaaatggaa ggttaaaaga atatatcggc ggctatcagg actacatcga
     3421 cgcaaaatca cgcgaagcga agattcagac ggaatccgca cccaaaacag ccgatgccga
     3481 accggtgaaa gaaaaaccca aagccaaccg cacggtcaaa ctgtcctaca aagaacagcg
     3541 agaactcgat gccctgcccg acgaaatcgc cgccttggaa gccgaacagg cagaaatcaa
     3601 cgcgcagctt tccgatcccg aaatcttcaa agattacgaa aaagccggcg cactgcaaag
     3661 ccgcgccgaa gaaatcgaaa tgctgctttt ggaaaagctg gaacgctggg agctgctgga
     3721 aaccaaacag aacggcaatg ccgtctgaaa tcaaacagga agaaaaaaat gttcaaacga
     3781 cctgaagaaa tcatcgtcct gatacttgcc gtcctctgga ttgccggtac gtattttctc
     3841 gccgccctgt ttggagcgga cgcttatacc gtgttaaaaa tcactgccct taccctgctt
     3901 tggtctgccg catcattcct cctttggcaa aaaaacctca gccggcatat ctggccgctg
     3961 ctgctcggct gcctgactgc ctgttggtgg ccgtatctga atcaatcggt cgcacccggt
     4021 tctttacctt ggtatgcatc atggacgttc aaaaccatct tagccctgat tcccgtaacc
     4081 gcaggttatc tgtttaaatg gaaacaatac cggcagcgca aaacaaagcc gtaagcccgt
     4141 ctttttcaga cggcatctcc ggtcagactg tcaacaattc cgataaaatg ctatactcgc
     4201 gcccgttgcc gcgccgtccg aaaccgccct ttgggaaagc agaaatgatc agcaaacaag
     4261 aataccaagc ccaagccgcc caaggctaca accgcatccc gctcgtgcaa gaactccttg
     4321 ccgatttgga tacgccgctt tccctctatc tcaaactcgc caaccgcccc tatacctacc
     4381 tgctcgaatc cgttgtcggt ggcgaacgtt tcggccgcta ttcttttatc ggcctgcctt
     4441 gcagccacta tctcaaagcc agcggcaaac acgtcgatgt ttatcaaaac ggcgaaattg
     4501 tcgagcaaca cgacggcaac cccctgccct ttatcgaagc cttccacaac cgcttcaaaa
     4561 cacccgaaat cccaagcctg ccgcgcttta ccggcggact ggtcggctac ttcggttacg
     4621 aaaccatcta caattttgaa cacttcgccc accgcctgaa aaacaccacc aaagccgacc
     4681 cgctcggcac gcccgacatc ttgctgatgc tgtcgcaaga attggcggtg atcgacaatt
     4741 tgagcggcaa aatccatctc gtcgtttacg ccgatccgtc gcagcccgac ggctacgaac
     4801 gcgcccgcga acgcctcgaa gacatccgca cccagttgcg ccaaagctgc gccatcccgc
     4861 tctcgctcgg cagcaaacac accgaagccg tcagcgaatt cggcgaagag ccgttcaaag
     4921 cctgcgttaa caaaatcaaa gactacatct tcgcaggcga ctgcatgcag gttgtcccca
     4981 gccagcgcat gagcatggaa ttcaccgaca gcccgctcgc cctctaccgc gccctgcgta
     5041 cgctcaaccc ttcgccttat ctcttttact acgatttcgg cgatttccac atcgtcggct
     5101 cctcgcccga aatcctcgtc cgacgcgaac gcaacgacgt tatcgtccgc cccatcgccg
     5161 gcacgcgcct gcgcggcaaa acccccgccg aagaccttgc caacgaacaa gatttgttaa
     5221 gcgatgccaa agaaatcgcc gaacacgtca tgttgattga cttaggacgc aacgacgtcg
     5281 gccgcatcag taaaaccggc gaagtcaaag ttaccgacaa aatggtgatt gaaaaatact
     5341 cccatgtaat gcacatcgtt tccaacgtcg aaggccgtct gaaagacggc atgaccaaca
     5401 tggacatcct cgccgctacc ttccctgccg gcacactctc cggcgcaccc aaagtccgcg
     5461 ccatggaaat catcgaagaa gtcgaaccga gcaaacgcgg catctacggc ggtgccgtcg
     5521 gcgtatgggg tttcaacaac gacatggatt tggcaatcgc catccgcacc gccgtagtaa
     5581 aaaacaacac gctatacgtc caaagcggcg cgggcgtggt tgccgactcc gatccggcct
     5641 ccgaatggca ggaaacacaa aacaaagccc gagccgtcat acacgccgcg caaatggtac
     5701 aggaagggtt ggataaatga cttatgaaaa actttatgag cttcgccaaa cactcagacc
     5761 gactaccaaa gacggtctct atacagataa tgaaaaaaat cgagaaatcc taacggttag
     5821 atggagcggc aacacagaaa atcctaaaaa tttctcagct gtcgctatag gaattaatcc
     5881 aagtaaagca aatgatgaaa gaagcgacaa aacccttacc caacttgcac gtttcttgga
     5941 tatgtacggt tttaccaact tcaaaatgtt aaatattttt tccagttact ctacacagca
     6001 aacaggaatt aggacaaaca cccaaaccga tttttccaaa tttaaaggat gtttggaaga
     6061 tgctgatatg attattcttg catggggtac agaccgcggt gcttataagg atgaaaaaaa
     6121 acagaatact ggaatttcta aaagcagaaa aatttatgga aaaagtattc tgtatttctg
     6181 aaactggtaa ttccagcgat acaagacatc cgtcaagaat tagttatagc tatcaacttg
     6241 tacaatttga agagtcagca tgaaaaacat atctctttct ccgcccgcca cgctcggaat
     6301 cctcggcggc ggacaattag gcagaatgtt taccgttgcc gccaaaacca tgggctacaa
     6361 agtaaccgta ctcgaccccg atccgaacgc tccggcggcg gaatttgccg accgccattt
     6421 gtgcgcgccg tttaacgacc agaccgcgtt ggacgagttg gcaaaatgcg cggcggttac
     6481 cactgaattt gaaaacgtca atgccgacgc gatgcgtttt ctggcaaaac ataccaacgt
     6541 ttcccccagc ggcgactgcg ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat
     6601 acgcaaagca ggtctgcaaa ccgcgccgta tcaagcagtt tgcaaagccg aagacatcac
     6661 tgaagcaagc gcgcaattcc tgcccggtat tctgaaaacg gccactttgg gctacgacgg
     6721 caaaggtcaa atccgcgtca aaacggtgga cgaactcaaa gccgcgtttg ccgaacacgg
     6781 cggcgtggat tgcgttttgg aaaaaatggt ggatttgcgc ggcgaaattt ccgttatcgt
     6841 atgccgtctg aacaatgaca acgtgcaaac tttcgatcct gccgaaaaca ttcacgaaaa
     6901 cggtatcctc gcctactcca tcgtcccagc ccgactgagt gccgacattc agcaacaggc
     6961 gcgacaaatg gcgcagcgtt tggccgatga attgaactac gtcggcgtat tggcggtaga
     7021 aatgtttgtt gtcggcgaca cgcatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca
     7081 caattccggc caccataccg tcgacgcctg cgcggcagac caattccagc aacaggtccg
     7141 cctgatgtgc aacctgccac ctgctgacac caaattgctg agttcttgct gtatggcgaa
     7201 tattttgggc gacgtttggc aggaagacgg cggcgaaccg gattggtttc ccctgcaaag
     7261 ccggccggac gcgcacctgc acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat
     7321 gggacacttt accgttttaa ccaccgattc ggacaccgca tttcaagaag caaaaaaact
     7381 gcatcagtcc ctataatccg accaacggca cagatgccgt ctgaagccgc cgacatactg
     7441 ccatgaacct gctgacactg ttacgccccg ccaccgtgca agactgcaaa gacatcttca
     7501 aagtccacct gcactccgtc caatacacct gcatcctcag ctacaacgaa cacgccctaa
     7561 aagtatggga aggcttgtta aacacagaaa gctacctgcc gaccatttcc gatcccgaca
     7621 aagcactttg ggttgccgaa tacaaaggca acatacaggg atttttccaa atcgactgcc
     7681 aagaagcaca actcgatgca ctttacgtcc accccctgtt ccacaacctc ggactgggca
     7741 ctgccctgct tcatcaggcg gaaaccatcg cccacaaatc tggtttgagc tttctgaaac
     7801 tctacgcatc gctcaactcc gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt
     7861 cggcagtatt gcagcttgac cccagcatca aaatcaaatg cgaactgatg cggaaacacc
     7921 tctgatccgt ccttataacc aaaactgata atcttcaaat caaacattat ttccacaagc
     7981 gcagacagcc gtataaagtt gccccatcta ccaaccgaca ggacaagcat atgaaaacct
     8041 acgcaccggc actctatacc gcagccctgc tcaccgcctg cagccccgca gccgattcaa
     8101 accatccgtc cggacaaaat gccccggcca ataccgaatc cgacggaaaa aacattaccc
     8161 tgctcaacgc ctcatacgat gtggcacggg atttttacaa agaatacaac cccttattta
     8221 tcaaaacata ccaatccgaa caccccggca catccgtcag catccaacag tcccacggcg
     8281 gctccagcaa acaggcatta tccgtagcca acggccttca agccgatgtc gtaaccatga
     8341 accaatcctc cgacatcgac ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac
     8401 aagccctccc cgaccacgcc gcgccctaca ccagcactat ggttttcctt gtccgaaaaa
     8461 acaaccccaa acagatccgc gattggaacg accttgccaa agacggcgtt aacatcgtca
     8521 tcgccaatcc caaaacctcg ggcaacggac gctacgcctt cctcggcgca tacggttacg
     8581 gtctgaaagc caccaacggc aacgaacagg aagcccaaaa actcgtcgca tccatcctca
     8641 aaaacacccc cgtttttgaa aacggcggac gcgccgccac caccaccttc acacaacgca
     8701 acatcggcga cgtactcatt acttttgaaa acgaagccaa ctacgtcagc aaaaaactga
     8761 cccaaggaca gtttgaaatc gtctatccga gctacaccat ttccgccgaa agccccgtcg
     8821 ccgtcgtcaa cagcgtcgtc gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg
     8881 aatacctttg gagcgaaccg gcgcaagaac tcgccgcctc actctacctg cgcccccgca
     8941 accccgaagt attggcaaga cacaaagccg acttcccaga cttagacacc ttctctcccg
     9001 aagaaaaatt cggcggatgg gacaacatca tgaaaaccta cttcgctgac ggcggcatat
     9061 tcgaccgcct gaccgcacaa aaatgaccgc tatgccgtct gaaccgcctt tcagacggca
     9121 ttccacacat caaaacagaa aaaccgcgcc caaaatcatc atcacaagac acaacggcaa
     9181 accggataca aaacccaaca ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat
     9241 ccttatctgg ggcataactt tccttaacca tcccttttga cagccaaata aacagtgctt
     9301 ttgcctgtca gaaacaaaac caatattttg cagttcgtat cggaacattt atagtggatt
     9361 aacaaaaacc agtacagcg
//
LOCUS       DAWGJV010000075         9323 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWGJV010000075 DAWGJV010000000
VERSION     DAWGJV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.075"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..143)
                     /locus_tag="WHQ94_001287"
     CDS             complement(<1..143)
                     /locus_tag="WHQ94_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128557.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGL"
     gene            complement(161..718)
                     /locus_tag="WHQ94_001288"
     CDS             complement(161..718)
                     /locus_tag="WHQ94_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ2128558.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WHQ94_001289"
     CDS             complement(794..2428)
                     /locus_tag="WHQ94_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ2128559.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2502..2831)
                     /locus_tag="WHQ94_001290"
     CDS             complement(2502..2831)
                     /locus_tag="WHQ94_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128560.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WHQ94_001291"
     CDS             complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WHQ94_001291"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ2128561.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4302..4733
                     /gene="rimP"
                     /locus_tag="WHQ94_001292"
     CDS             4302..4733
                     /gene="rimP"
                     /locus_tag="WHQ94_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ2128562.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            4761..6263
                     /gene="nusA"
                     /locus_tag="WHQ94_001293"
     CDS             4761..6263
                     /gene="nusA"
                     /locus_tag="WHQ94_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ2128563.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6275..9163
                     /gene="infB"
                     /locus_tag="WHQ94_001294"
     CDS             6275..9163
                     /gene="infB"
                     /locus_tag="WHQ94_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ2128564.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2496 a         2688 c         2366 g         1773 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttccgccga acgcatcgag cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg catttcgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt acaccgataa ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttt aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttgg tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aggtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtacc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccggc cgggcggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagat ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggcg gctgtgcggg gggttccgcc cgccattcaa acgtccggca
     2581 gacataaaaa catcgtaagc aagattcgaa ccggtctgca accgcccctg ccagaaaaac
     2641 gggcaaagca tttcatattg gaaaaacccg gccgcgccgc cgacgggaca gtccggcaca
     2701 aacagcatca cgcccggatt gaaaaggacg gatagccgcc ggcagcacaa tcttaccacc
     2761 tccaaaacgc cgccggagaa cgccgaaaca gccgatgccg tctgaagccg cttcagacaa
     2821 catcgggaca tcaaccgtaa cgccgttgga aatcgcgcat aaaatctgcc aaagcccgca
     2881 cgccttcaag cggcatcgca ttataaatgc tggcacgcat accgccgacg gttttatagc
     2941 ccttaagcag gcacaagccc tgcaattcgg cttccagcac aaaacggcgg tcaagctcct
     3001 catcccccgt ttggaacacg acattcattt tagaacgcgc attcggacgg atacggttga
     3061 tatagaaacc gccgctgcca tctatcgtct catacaaggt ttgcgccttc agccgattga
     3121 ccgcttcaat ttttttcaca ccgccctgcg cctgcagcca gcggaacacc agccccgaca
     3181 tataaatcgc gtaagttgac ggcgtgttgt acataccgtc gcggttcaaa tgcgaacggt
     3241 agttgaacac atcgggaata tcgttcggac aacgctcgag caaatcctca cgcacaatca
     3301 ccaccgtaac ccctgccggc ccgatgtttt tttgcgcgcc tgcgtaaatc agtccgtagt
     3361 cggcaacatc aaactcacgc gacagaatct cgctggacat atcgcacacc aacggcggca
     3421 tgccgtctga aaggcgaggc acttcacggt attgcagccc gttgaccgtt tcattgacgg
     3481 caaaatggac aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc ggcaggtcga
     3541 gatagtcgaa ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg gtcaaacggc
     3601 tcatctgttc ataagcgata cggctccagt tgcccgttac caccgcgtcg gcagtgcgga
     3661 aaccgtgtgc cagattcatg gctgccatat taaattgggt tgttgctcca ccctgcagaa
     3721 acaatatctt atagttgtca ggcactttca aaagctgcct caaatcctgt tccgcatgat
     3781 gcaggatgct caaaaacatt tccgaacggt ggctcatcgc catcacagga aaacccgtac
     3841 cgttgtagtc caacatttcc tgccgcgccg tttccaacac ggcttcgggc aatacggcag
     3901 ggccggcgga aaaattgtaa atcggataaa gagacataat gcagccttga ttctgaacaa
     3961 taacccgccc gattttaggc ttgcagcagg ctttgtgcaa ggatgggaaa tacctgtcct
     4021 ccgcccgatt ccatgccgcc cgaacacggg aaacaacatc aatatattga tttacaaata
     4081 taaaaatcat gctagcgaca aatagataca tttgttttgt caacaatatt cacgatttcc
     4141 cattacaaac ctcccttaca cccgcttttt tccgtcccaa aaacacaaaa taaatcaaca
     4201 ctttcatttc tccgcaaaag cggttataat cacgccgatt tttcaacttt gacgaaaaat
     4261 ggccacgggg ccattttttt attatgtaca tagggagcag catggatatt caaaccatcc
     4321 tcgaaaaaac cctgcccggc ctgggctacg aactggtcga tttcgaactg accgcgcaag
     4381 gaacattgcg cgtgttcatc gataaagaag gcggcattac cgtcgaagac tgcgcaaccg
     4441 tcagcaacca cttgagccgc gtcttcatgg tggaagacat cgactacaaa aacctggaaa
     4501 tttccagccc cggactcgat cgccccttga aaaaagccgc cgacttcgtg cgctttgccg
     4561 gtcagaatgc caaaatcaaa acccgcctgc cgatagatgg tcagaaaaac tttatcggta
     4621 aaatcgaagg ctgcgaaaac gataccgtta ccatatcctt cgacggcaaa accgtacaaa
     4681 tcgaattggg caacatcgac aaagcccgcc tgcgccccga attcaaattc taaaacacaa
     4741 caatattgga gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa gcactggcaa
     4801 gcgaaaaaaa cgttgatgcg gaagtcgtct tccaagcact ggaattcgcc ctgtctaccg
     4861 ccgccaagaa aaaggcagac cgcgaacaca tggatgtccg cgtacaaatc aaccgcgaca
     4921 ccggcgaata ccaaaccttc cgccgttggc tgattgtcgc cgatgaagac tatacctatc
     4981 ccgatgtcga aaaaaccatc gaggaaatcc aagaggaaat tcccggcacc gccatccaaa
     5041 tcggcgaata ctatgaagag cagctgccca acgaaggctt cggccgccaa gccgcgcaaa
     5101 ccgccaaaca aatcatcctg caacgcatcc gcgatgccga gcgcgagcag aatctgaacg
     5161 agtttctcgc cgtcaaagaa gacatcgtgt ccggcacggt caaacgcgtc gaacgccacg
     5221 gcatcatcgt cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc gaccaaatga
     5281 ttccgcgcga aaacttccgc agcggcgacc gcatccgcgc cctcttcctg cgcgtcgaag
     5341 aaatcggcaa caccggccgc aaacaagtca tcctgagccg tacttccggc gatttcctcg
     5401 tcaaactgta cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa atccgcgctg
     5461 tcgcccgcga cccgggacaa cgtgccaaag tcgccgtcaa agccaacgac cagcgcatcg
     5521 atccgcaagg cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc agcaacgaat
     5581 tgtccggcga gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa ttcgtcatga
     5641 gcgcgctctc acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa cacgccgtcg
     5701 atgtcatcgt tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt caaaacgtgc
     5761 gccttgcttc cgacctgacc ggctggcagc tcaacatcat gacttccgcc gaggcagacg
     5821 aacgcaatgc ggcagaagat gccgccatcc gccgcctgtt tatggatcac ttgaacgtgg
     5881 acgaagaaac cgccgacgta ctggttcagg aaggttttgc aaccttggaa gaagtcgcct
     5941 atgttcctgc cgccgaactg cttgccattg aaggatttga cgaagaaatc gtcgatatgc
     6001 tccgcaaccg cgcccgcgat gccatcctga ccatggcgat tgccgccgaa gaaaaactgg
     6061 gcgaagtatc cgacgatatg cgcaacctcg aaggcataga tgccgatatg ctccgcagcc
     6121 ttgccgaagc aggcattacc acccgcgacg acttggcaga gcttgctgtg gacgaactga
     6181 ttgaaatcac cggtgtaaac gaagaaaccg caaaagccgt catcctgacc gcacgcgaac
     6241 actggtttac cgaagacaaa taaagggggt acagatgagt aacacaaccg tagaacaatt
     6301 tgccgccgag ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt
     6361 cagcaaaaac agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta
     6421 cctgaccaag aaaaacggca gcaacggcgg caccatcagc atccgccgca ccaaaaccga
     6481 agtcagcacc gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat
     6541 tccttctgcc gaagaattgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt
     6601 ccggccggag cagacggcag aagacgcggc aaaagcccga gccgaagctg ccgcacgtgc
     6661 agaagcccgt gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa
     6721 caaagccaaa cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc
     6781 ggcggaaacc aaacccgccg aagaaagcaa agcggaaaaa gcccaagccg acaaaatgcc
     6841 gtctaaaaaa cccgtcgagc ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg
     6901 caaaggcaaa gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt
     6961 ggtcagcgcg gaagaacagg cgcaacgcga tgaagaagca cgccgtgccg ccgcacttcg
     7021 cgcccaccag gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat
     7081 gaaacaacag gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg
     7141 tcccgccaaa cctgccgaga aaccgcaggc agccgcgccg gccgtcgaaa acaaacctgt
     7201 caatccggca aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg
     7261 caacgccaaa ggcaaaggcg gaaaaggcgg gcgcgaccgc aacaatgcac gcaacggcga
     7321 cgacgagcgc gtacgcggca gcaaaaaagg caaaaaactc aaactcgagc cgaatcaaca
     7381 cgccttccaa gcaccgaccg aacccgtcgt tcatgaagtt ttggttcccg aaaccattac
     7441 cgttgccgat ttggcgcaca aaatggcggt caaaggcgtg gaagtggtca aagccctgat
     7501 gaagatgggc atgatggtaa ccatcaacca atccatcgac caagacaccg ccctgattgt
     7561 cgtcgaagaa ctcggacaca tcggcaaacc cgccgccgcc gacgatcccg aagccttctt
     7621 ggacgagggc gcggaagcgg tggaagccga agcattgccg cgtccgcccg tcgttaccgt
     7681 catgggacac gtcgaccacg gcaaaacctc gctgctggac tacatccgcc gtgccaaagt
     7741 ggtacagggc gaagcgggcg gcattaccca acacatcggt gcataccacg ttgaaacccc
     7801 acgcggcgtg attaccttct tggatactcc gggtcacgaa gcgtttaccg ccatgcgcgc
     7861 gcgcggcgcg aaagcgaccg acatcgtgat tctcgtggtt gccgcagacg acggtgtaat
     7921 gccgcaaacc atcgaagcca ttgcacacgc caaagcggca ggcgttccga ttgtggttgc
     7981 cgtcaacaaa atcgataaag acactgccaa ccccgaacgc atccgccagg aactgaccca
     8041 acacgaagtg atccccgatg attggggcgg cacggttcaa ttcatcgacg tttctgcgaa
     8101 aaaaggaaca aacatcgacg cgctgctcga agcagtcttg ctcgaagccg aagtattgga
     8161 actgaccgca cctgtcgatg cgcccgccaa aggcatcatc gtcgaggcgc gcttggacaa
     8221 aggccgcggc gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgacat
     8281 gctgctggcc ggtacggcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc
     8341 cattaccgaa gccggcccgt ccatccccgt cgaaatcctc ggcttgtccg acgtaccgaa
     8401 tgcgggtgaa gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt
     8461 ccgccaaggc aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa
     8521 tatgttcaac aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga
     8581 cgtgcagggc tcttacgagg ctttggcggg cagcctgaaa aaactgtcca cagacgaagt
     8641 gaaagtgaac gtgttgcaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc
     8701 catcgcttcg ggcgcattca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa
     8761 acttgccgaa aatgaaaacg tggaaatccg ctactacaac atcatctacg atgccatcga
     8821 cgacgtgaag gcggcgatga gcggtatgct ttccccggaa gagaaagaac aggttaccgg
     8881 tacggtcgaa atccgtcagg tcatctccgt ttccaaagtc ggcaacattg caggctgtat
     8941 ggttaccgac ggcgtggtca aacgcgattc ccatgtccgc ctcatccgca acaacgtggt
     9001 tatccacacg ggcgaactgg cttcgttgaa acgctataaa gacgatgtca aagaagtccg
     9061 catgggcttc gagtgcggtc tgatgctcaa aggctacaac gaaatcatgg aaggcgacca
     9121 actggaatgc ttcgacatcg tcgaagttgc ccgcagcctg taattccttt gcaaataaaa
     9181 tgccgtctga agcgttcaga cggcaacgaa acgggttctg tatcatacag aacccgtttt
     9241 ttgtcgcaaa tcggcttcag acagccctct tgccttatcc cgatttgaat ctgacttgcc
     9301 atacaaacag gcttcagacg gca
//
LOCUS       DAWGJV010000076         9262 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWGJV010000076 DAWGJV010000000
VERSION     DAWGJV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.076"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            160..1503
                     /locus_tag="WHQ94_001295"
     CDS             160..1503
                     /locus_tag="WHQ94_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ2128565.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1506..2738
                     /locus_tag="WHQ94_001296"
     CDS             1506..2738
                     /locus_tag="WHQ94_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ2128566.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2731..3507
                     /locus_tag="WHQ94_001297"
     CDS             2731..3507
                     /locus_tag="WHQ94_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ2128567.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKTEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3507..4133
                     /locus_tag="WHQ94_001298"
     CDS             3507..4133
                     /locus_tag="WHQ94_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ2128568.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4137..4730
                     /gene="nqrE"
                     /locus_tag="WHQ94_001299"
     CDS             4137..4730
                     /gene="nqrE"
                     /locus_tag="WHQ94_001299"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ2128569.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4744..5961
                     /gene="nqrF"
                     /locus_tag="WHQ94_001300"
     CDS             4744..5961
                     /gene="nqrF"
                     /locus_tag="WHQ94_001300"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ2128570.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6114..7169
                     /locus_tag="WHQ94_001301"
     CDS             6114..7169
                     /locus_tag="WHQ94_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ2128571.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7195..7410
                     /gene="nqrM"
                     /locus_tag="WHQ94_001302"
     CDS             7195..7410
                     /gene="nqrM"
                     /locus_tag="WHQ94_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ2128572.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7534..8112)
                     /gene="grpE"
                     /locus_tag="WHQ94_001303"
     CDS             complement(7534..8112)
                     /gene="grpE"
                     /locus_tag="WHQ94_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ2128573.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8296..9114
                     /gene="cysE"
                     /locus_tag="WHQ94_001304"
     CDS             8296..9114
                     /gene="cysE"
                     /locus_tag="WHQ94_001304"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ2128574.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2232 a         2592 c         2293 g         2145 t
ORIGIN      
        1 gcttccgcaa ggaaaaaagc agcaaatctg acaaacaggt acaattcctg atagaatcta
       61 acactttgct acattaattt attcaatgct tttgatgcca cgcgacacgg tatcaaaaca
      121 cgccgttttt accttaacca tcacaagtaa tgcaaatata tgattaaaat caaaaaaggt
      181 ctaaacctgc ccatcgcggg cagaccggag caagtcattt atgacgggcc cgtcattacc
      241 gaagtcgcgt tgcttggcga agaatatgcc ggtatgcgcc cctcgatgaa agtcaaggaa
      301 ggcgatgccg tcaaaaaagg ccaagtgctg tttgaagaca aaaagaatcc gggcgtggtg
      361 tttaccgcgc cggtttcagg caaaatcgcc gccatccatc gcggcgaaaa gcgcgtactt
      421 cagtcggtcg tgattgccgt tgaaggcaac gacgaaatcg agttcgaacg ctacgcgccc
      481 gaagcgttgg caaacttaag cggcgaagaa gtgcgccgca atctgatcca atccggtttg
      541 tggactgcgc tgcgtacccg tccgttcagc aaaatccctg ccgtcgatgc cgagccgttc
      601 gccatcttcg tcaatgcgat ggacaccaat ccgctggctg ccgaccctac ggtcattatc
      661 aaagaagccg ccgaggattt cagacgaggt ttgctggtat tgagccgttt gaccgaacgc
      721 aaaatccatg tgtgtaaagc agcaggcgca gacgtgccgt ctgaaaatgc tgccaacatc
      781 gaaacacatg aattcggcgg cccgcatccg gccggtttga gtggcacgca cattcatttc
      841 attgagccgg tcggtgcaaa caaaaccgtt tggaccatca attatcaaga tgtaattgcc
      901 atcggacgtt tgtttgcaac aggccgtctg aacaccgagc gcgtgattgc tttgggtggt
      961 tctcaagtca acaaaccacg cctcttgcgt accgttttgg gtgcgaaagt atcgcaaatt
     1021 actgcgggcg aattggttga cgcagacaac cgcgtgattt ccggttcggt attgaacggc
     1081 gcgattacac aaggcgcgca cgattatttg ggacgctacc acaatcagat ttccgttatc
     1141 gaagaaggcc gcagcaaaga gctgttcggc tgggttgcgc cgcagccgga caaatactcc
     1201 atcacgcgta cgaccctcgg ccatttcctg aaaaacaaac tcttcaagtt cacgacagcc
     1261 gtcaacggtg gcgaccgcgc catggtgccg attggtactt acgagcgcgt aatgccgcta
     1321 gacatcctgc ctaccctgct tttgcgcgat ttaatcgtcg gcgataccga cagcgcgcaa
     1381 gcattgggtt gcttggaatt ggacgaagaa gacctcgctt tgtgcagctt cgtctgcccg
     1441 ggcaaatacg aatacggccc gctgttgcgt aaggtgctgg aaaccattga gaaggaaggc
     1501 tgattatggg cttgaaacat tttctggaaa aaatcgaacc gcacttcctg ccgggcggca
     1561 aacatgaaaa atggtatgcc ctctacgaag ctgcggcgac gattttctat acatccggtg
     1621 cggtaacgcg caaagcggca cacgtccgcg atgcgctcga ctccaaacgc atgatgattt
     1681 tggtgtggct ggctttgttc cctgccatgt tctacggtat gtacaacgtc ggcgcacagg
     1741 cattcggtgc gttaacgccc gatttgctgc aacaaagcat cgccaacgac tggcattacg
     1801 cccttgccaa cgctttgggc atcaatatgt cgtctgaagc gggcgtgttg ggcaaaatgc
     1861 tgttcggcgc gatttacttc ctgccgattt acgcgaccgt atttattgtc ggcggtttct
     1921 gggaagtttt gttcgccacc gtgcgcaaac atgaaatcaa cgaaggtttc tttgttacct
     1981 cgattctgtt tgccttaatc gttccgccca cgctgccgct gtggcaggca gctttgggta
     2041 tttctttcgg cgttgtggtt gcgaaagaag tattcggcgg tacaggtaaa aacttcatga
     2101 accctgcgct ggcaggccgt gccttcctgt tcttcgccta ccctgccaac ttgagcggcg
     2161 atgcggtttg gacggcggtt gacggctatt ccggcgcaac cgcgctggcg caatgggcgg
     2221 cacacggtgc agacggcctg aaaaacgccg taaccggtca aaccatcact tggatggatg
     2281 cgtttatcgg caaactgccc ggctccatcg gcgaagtctc cactttggca ctcttaatcg
     2341 gcggcgcgtt tatcgtgttt gcccgcatcg cttcttggcg cattattgcc ggcgtgatga
     2401 tcggtatgat tgcgatgtct tcgctgttca acttcatcgg ttcggacacc aacgctatgt
     2461 ttgctatgcc ttggtactgg cacttggtgg tcggcggctt cgccatcggt atgctgttta
     2521 tggcgaccga ccctgtttcc gcttccttta ccaatgtcgg caaatggtgg tacggcgcac
     2581 tgatcggtgt gatgtgcgta ttaatccgcg tggtcaatcc ggcttacccc gaaggcatga
     2641 tgttggcgat tctgtttgcc aacctgtttg ccccgatttt cgactatttc gtcgcacaag
     2701 cgaacatcaa acgcagaaag gcgcgcagca atggctaaga aattcgataa agacagcttc
     2761 agcggcacgc tgattgtcgt gttggcggtc agcctgattt gctcggtcat cgttgccggt
     2821 gcggtcgtcg gcttgaaacc catccaagag aaacaaaaac tccaagacaa acaaggctat
     2881 atcttgagcg tagccggttt gatggataag gacaccgaca tcggtaaaac ctttgccgag
     2941 cgtatcgagc aacgtgttgt cgatttggcg accggcgaat acgtcgccga tgcgcctaaa
     3001 gacttcagcg cgcgcatcgc aggcaaagac cctgcccaaa gcatccgcat caaaaccgaa
     3061 gacgatttgg caggcatcaa aagccgtgcc aaatacaccg aggtttattt ggtaaaaggc
     3121 gaagacggca aaatcgggca aatcatcctg cctatgcacg gcaacggttt gtggtcggtc
     3181 atgtacggct ttgtcgccat ccagcccgac ggcaacacca tcaacggcat tacctactac
     3241 gaacaaggcg aaaccccggg cttgggcggc gaaatcggca atccgttgtg gcagcaaaaa
     3301 ttcgtcggca aaaaactgtt tgacggacaa ggcaaactcg ccctgcacgt cggcaaaggc
     3361 gcgggttcgg acaaagaaca cggcgtagat gccctctccg gcgcatcgct gacatccaaa
     3421 ggcgtgcaag gttcgttcgc ctactggttc ggcgaaaacg gctatatccc ctacctgaac
     3481 aaattgaaat cagcaggagc gcaataatgg ctgatatgaa acgcttgaaa catttgatgt
     3541 tttcaccctt tatcgacaac aacccgattg ccttgcaggt tttgggtatt tgttcggctt
     3601 tggcggttac caccaaactt cagacggcca tcgtgatggg tatttccgta gctttggtaa
     3661 ccggtttttc cagcttcttc atttcgctgg tacgcaacta catccccaac agcatccgta
     3721 ttatcgtgca aatggcgatt atcgcgtcgc tggttacgct ggtcgaccaa ctcttgcagg
     3781 catttgccta cgaattgtcc aaacagcttt ccgtattcgt cggtctgatt attaccaact
     3841 gtatcgtgat gggccgcgcc gaagcatttg cgatgaaaga gccgccgctg gaaagcctga
     3901 tcgacggcat cggcaacggc gcgggctacg ggataatgct gcttgtcgtc gccaccgtcc
     3961 gcgaactgat tggctcgggc aaactcttgg gctacaccgt tttccaaacc gtgcaggacg
     4021 gcggctggta tcagaccaac ggcttgttcc tgctcgcccc cagcgcgttc ttcatcatcg
     4081 gctttttgat ttggggactg cgtacctgga aacccgaaca ggcggaggaa taagctatgg
     4141 aacactattt gagcctcttc atcaaatccg tcttcattga aaatatggca ctatccttct
     4201 ttttgggtat gtgcacgttt ttggcggtat ccaaaaaagt atccaccgca ttcggtttgg
     4261 gtgtggcggt cattttcgta ctcgggctgt ccgtccctgt caaccaactc gtttactcgc
     4321 tgctcaaaga cggcgcgatt gtcgaaggcg tggatttgac ctttttgaaa ttcatcacct
     4381 tcatcggcgt gattgccgct ttggtgcaga ttttggaaat gttcttggat aaattcgtcc
     4441 ccgccctcta taacgcattg ggcatctacc tgccgctgat taccgtgaac tgcgcgattt
     4501 tcggcgccgt atcgtttatg gcgcaacgcg aatacaactt cggcgagtcc gttgtatacg
     4561 gcttcggcgc gggcttgggc tggatgttgg cgattgtcgc tttggcgggc attaccgaaa
     4621 aaatgaaata ttcggacgct cccaaaggcc tcaaaggctt gggcattacc tttatcgccg
     4681 ccggcctgat ggcgatggcg tttatgtcgt tctctggcat ccagttataa gaaagggatc
     4741 ggtatggaga ttattttagg tatcgtgatg tttaccgtca tcgttttggt tttggcactg
     4801 atgattctgt ttgccaaatc caaactggtc agcgaaggcg acatcaccat caaagtcaac
     4861 ggcgaaaaag agctgacgat gcccgccggc ggcaaactct tgggcgcgct tgccaacgaa
     4921 ggcatcttta tcccctccgc ctgcggcggg ggcggttcgt gcggacaatg ccgcgtcgtc
     4981 gtaaaaagcg gcggcggcga catcctgccg accgagctgt cccacatcag caaacgcgaa
     5041 gcacgcgaag gctgccgtct gtcgtgccaa gtcaacgtca aaaccgacat ggacatcgaa
     5101 gtccccgaag aagtgttcgg cgtgaaaaaa tgggaatgca ccgtcatctc caacgataac
     5161 aaagccacgt tcattaaaga actcaagctt gccattcccg aaggcgaaga agtccccttc
     5221 cgcgccggcg gctacattca aattgaagct cctcctcaca ctgttgccta caaagacttc
     5281 gacattccta aggaatatca cgaagactgg gacaaataca atttgtggca atacgtttcc
     5341 aaagtggacg agccgatttt gcgcgcctac tctatggctt cgtatcctga agaaaaaggc
     5401 atcattatgc tgaacgtgcg tatcgccacg ccgcccccgc gcgtacctga tgcgcctccg
     5461 ggacaaatgt cgtcctacat ctggtcgctc aaacccggcg acaaagttac gatttccggc
     5521 ccgttcggcg aatttttcgc caaagacacc gatgccgaaa tggtatttat cggcggcggt
     5581 gcgggtatgg ctccgatgcg ttcccacatt ttcgaccagt tgaaacgttt gaactccaaa
     5641 cgtaagatta ccttttggta cggcgcacgt tccaaacgcg agatgttcta tgtcgaagac
     5701 ttcgaccaac tcgcagcaga attcccgaac ttcacgtggc acgtcgccct gtccgacccg
     5761 ctgccggaag acaactggga cggctacacg ggcttcatcc acaacgtggt ttacgaaaac
     5821 catctgaaaa accacgaagc accggaagac tgcgaatttt atatgtgcgg tccgccaatc
     5881 atgaaccagt ccgtgatcaa aatgctcaaa gacttgggcg tggaagacga aaacatcctc
     5941 ttggatgatt tcggcggttg agtattcggc ttcaaataca aaaggaagaa acctcggttt
     6001 cttccttttt ctatgccgat gccatccgaa cacccttcag acggcatcgg catagaaaca
     6061 taccggccgt tttgcgatat aattcaaacc atttgccaaa accgtcaaac actatgccgt
     6121 ctgaaacacg cctgccgaac tttatccgca ccttgatatt tgccctgagt tttatcttcc
     6181 tgaacgcctg ttcggaacaa accgcgcaaa ccgttaccct gcaaggtgaa acgatgggca
     6241 cgacctatac cgtcaaatac ctttcaaaca atcgggacaa actcccctcc cctgccgaaa
     6301 tacaaaagcg catcgatgac gcgcttaaag aagtcaaccg gcagatgtcc acctatcagc
     6361 ctaattccga aatcagccgg ttcaaccaaa ccacggccgg caaacccctc cgcatttcaa
     6421 gcgacttcgc acacgttact gccgaagctg tccgcctgaa ccgcctgaca cacggcgcgc
     6481 tggacgtaac cgtcggcccc ttggtcaacc tttggggatt cggccccgac aaatccgtta
     6541 cccgtgaacc gtcgccggaa caaatcaaac aggcggcatc ttatacgggc atagacaaaa
     6601 tcattttgaa acaaggcaaa gattacgctt ccttgagcaa aacccacccc aaggcctatt
     6661 tggatttatc ttcgattgcc aaaggcttcg gcgttgataa agttgcgggc gaactggaaa
     6721 aatacggcat tcaaaattat ctggtcgaaa tcggcggcga gttgcacggc aaaggcaaaa
     6781 acgcgcgcgg cgaaccgtgg cgcatcggta tcgagcagcc caatatcgtc caaggcggca
     6841 atacgcagat tatcgtcccg ctgaacaacc gttcgcttgc cacttccggc gattaccgta
     6901 ttttccacgt cgataaaaac ggcaaacgcc tctcccatat catcaacccg aacaacaaac
     6961 gacccatcag ccacaacctc gcctccatca gcgtggtcgc agacagtgcg atgacggcgg
     7021 acggcttgtc cacaggatta ttcgtattgg gcgaaaccga agccttaaag ctggcagagc
     7081 gcgaaaaact cgctgttttc ctgattgtca gggataaagg cggctaccgc accgccatgt
     7141 cttccgaatt tgaaaaactg ctccgctaaa catacaaacc gattaggaaa caccatgaaa
     7201 accctgctcc ttaccttcgg catcttcctg accgtcatca tcggtatggc ggtcggctat
     7261 attttctcca aacgcagcat caaaggcagt tgcggcggca ttactgcctt gggtatgaaa
     7321 aaaatgtgcg actgcgacac accttgcgac accctgcaaa agaaactgga tgaagaaaaa
     7381 caggcaggcg gcatacgggt tgaccgttaa caccgaaaca aaatgccgtc tgaagcccga
     7441 caggcgggtt cagacggcat tgccgcttta cttcgatcgg tttatacgca aaacgcgctt
     7501 caggaaataa atcagattat tccccagacg ccttcaggct tccttctgtg ccaccgtaac
     7561 catagccggg cgcaacacgc ggtcggacag cgtataaccc ttcttcatca cacccaccac
     7621 ggtattcggc tcctgttcgc ttgccaccgc ctgcatcgcc tgatggatat tcggatcgag
     7681 cttatcgccc gctttagggt tgatttcctt gatttgcgta gcatcaaatg ctttctgcaa
     7741 ctcgttcaaa gtcatctgca cgcccatttt cagcgcatcg aaattgccgc tctgatccaa
     7801 aagcgccatt tccagataat ccttgaccgg cagcatttcc acggcaaact tctgtccggc
     7861 gaacttgtgc gtatccgcaa tttcctgctg gtggcggcgg cgcaggtttt gctcgtttgc
     7921 caaagcgcgc agctgctcgt ctttcaactg cgcttccagc tcggcaatcc gcgcctgcaa
     7981 atcctcataa gccggctctg cggcagcctg ttcctgcaca ccgtccgcat ttcctactgt
     8041 ctcgacggtt tccaccgcct ccacattttc aaccgcttct tcactgtttt gctgctgtgt
     8101 ctgttcgctc atatcgtatc ccttaaaaca aattggaaat caaaatccag ttattacccg
     8161 cgcaagataa ggacattttc aagaaacttc aagcaaaagc agagaatttc aaattcattt
     8221 tcaaatcccc taccgaaaaa ataatataga cggtaagata ctgccgacca tatcgatttt
     8281 caaacgtcat cactcatgaa aaaagaccat ttaaacacaa ccggtttcga cctctggcac
     8341 accatccgcg aagaaaccgc ggccgcggtc tccgccgaac cgatgctggc aagttttttg
     8401 caccaaaccg tgttgcgcca cgagtccctc ggctccgtcc ttgcctacca cctttccagc
     8461 aaactcggca gcccgattat ggacgtgcgc gcgctgtttg aaatctacca gcaggcgttg
     8521 ggcagcgaca cccaaatcgg caaatgcgtc gaagcagact taaaagccat ctacgaacgc
     8581 gatcccgcct gcgacgaata ttcgctgccg cttttatatt tcaaaggctt ccacgcgatt
     8641 caggcacacc gcatcaacca ccggctgtat ctcgacgggc gcaaaacgct ggcgtatttc
     8701 ctgcaaaacc gtatgtccga agtattcggc gtggacatcc atcccgccgc ccgtttcgga
     8761 tacgggctga tgctcgacca cgccaccggc tttgttgccg gcgaaaccgc cgtgttgggc
     8821 aacaatattt cgattttgca cggcgtaacg ctcggcggtt cgggcaaaga aggcggcgac
     8881 cgccacccta aaatcggcga cggcgtgatg atcggcgcaa acgcctcgat attgggcaat
     8941 atccgcatcg gcagcaatgc caaaatcggg gcgggcagcg tcgtggtttc agacgtgccg
     9001 ccgtccatca cggttgtcgg cgtacccgcc aaacccgtgg cgcgatcgct caaaacaccg
     9061 tcggcggata tggatcaaaa tatccagttt accgaaatcg actttatgat ttgaatatcc
     9121 gcaccaatgc cgtctgaaac gccaaacgtg cttcagacgg cattgcccgc ttcaagcctg
     9181 cgccttgata tgccgccgat gccggcactt gccagtcgga caaaatgcct tttattcgat
     9241 acacccgatt cccacgcaac cg
//
LOCUS       DAWGJV010000077         9144 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWGJV010000077 DAWGJV010000000
VERSION     DAWGJV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.077"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..377)
                     /locus_tag="WHQ94_001305"
     CDS             complement(<1..377)
                     /locus_tag="WHQ94_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2128575.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDNTLSELLELINR"
     gene            418..1257
                     /locus_tag="WHQ94_001306"
     CDS             418..1257
                     /locus_tag="WHQ94_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128576.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            complement(1527..2789)
                     /locus_tag="WHQ94_001307"
     CDS             complement(1527..2789)
                     /locus_tag="WHQ94_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ2128577.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(2791..4887)
                     /locus_tag="WHQ94_001308"
     CDS             complement(2791..4887)
                     /locus_tag="WHQ94_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ2128578.1"
                     /translation="MPTYLSRLSFSFSRRLPVILQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIMIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLMTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYAENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            complement(5104..5439)
                     /locus_tag="WHQ94_001309"
     CDS             complement(5104..5439)
                     /locus_tag="WHQ94_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128579.1"
                     /translation="MYELSIVDLELVSGAGPIADFTTGEGGAIGGGTGYIAGKAGGGT
                     IGGILGSPFGPGGTIVGVAAGQQYGGKVGSVIGAGAGSAIGGVIGRGIESILDNDRSG
                     NDYGDGTGY"
     gene            complement(6032..6367)
                     /locus_tag="WHQ94_001310"
     CDS             complement(6032..6367)
                     /locus_tag="WHQ94_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ2128580.1"
                     /translation="MKELNISDLKIVSGGAEPAYINPLDAIGIGSAAVGGGRAVSATA
                     TGDMIARSAARGGLIGLAGIAGWNTGTYLYHETPLGDWLSSGVDKLTGLDQNFNDASG
                     NDYGDGTGY"
     gene            complement(6609..7757)
                     /locus_tag="WHQ94_001311"
     CDS             complement(6609..7757)
                     /locus_tag="WHQ94_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ2128581.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKKALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDTIAKQVAQFAQAQTVVSGYLAFGSSSSYRNHWDTRDVFAVQLIGKKHWTISAPN
                     FDMPLYMQQAKDMPHITPSKTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIGWEHDQKNLDDSAQAVTEMMNNPKNYQAFMQ
                     DFLGNQRTNTAFNMDLFGNAHNQTLPEHCFLRLNSNDCSTLPQGFLIVNGIKLNVDES
                     SMKFLTILVDKYMISLAEILLFFSVDEEENIKKLVWRLGELDIIEIIR"
     gene            complement(7933..8136)
                     /locus_tag="WHQ94_001312"
     CDS             complement(7933..8136)
                     /locus_tag="WHQ94_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEZ2128582.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            complement(8179..8586)
                     /locus_tag="WHQ94_001313"
     CDS             complement(8179..8586)
                     /locus_tag="WHQ94_001313"
                     /inference="COORDINATES: protein motif:HMM:NF025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEZ2128583.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            complement(8599..8823)
                     /locus_tag="WHQ94_001314"
     CDS             complement(8599..8823)
                     /locus_tag="WHQ94_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2128584.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
BASE COUNT         2919 a         1861 c         1601 g         2763 t
ORIGIN      
        1 cggttaatca gttccagcaa ttcggacagg gtgttgtctt gcgccagcca gttacggtag
       61 cggcataagg tgctgtaatc ggggatgctc agttcgtcaa aacgacaaaa caggttgaaa
      121 tcgatgcggg tgatgaggct gtgttcgagt tcgggatcgg aaaggctgtg ccattgtccg
      181 agcaggacgg ctttgaacat ggataacagg ggataggcgg gacggcctcg gtggtctcga
      241 aggtaacggg ttctttgacg attcaggtac tgttcgatcg gttgccaatc aatcacctga
      301 tccaacttca atagtgggaa gcggtcgatg tgtttggcaa tcatggcttg tgcggtttgc
      361 tggaagaagg tactcatgag aaatccccta aatgtcttag taggaattta ggggattttg
      421 gggaattttg caaaggtctc aaattgtgaa ttctgggata aaattaatta taatcgggct
      481 gcccaagccg ccgagcagcg ggcaaagcag gcgcaggaaa caccgaaaga gccgccgaaa
      541 caggaagagc actttatcgc caagctgccg aacgacaagc taatgacgat atacaggctg
      601 tttcaaaaac aactggacga catgcgcggc ttggagcgcg atatagaacg cggagattgg
      661 tacatcaatg cgcgataccg catgaacgaa aaccgaaaca aaataagggc gctggaagac
      721 gacaacaaac ggctgcatca cgactattgg gcagtccaag accaaggcga aaaccgcttt
      781 ttcggcatgg gtaaaatcgg cgcaaaactg gttaaggaaa acgagaagca gcaacacatc
      841 aacgaacggc atggagcgaa cgtgcgagaa atccggcggc tgaaaacaga aaacgaaaaa
      901 ctggaagggc agtatagcga caagttggcc gccgacgtgg tcaaagtacg cgacaggttt
      961 aacgaagaac tggacaatgt ccgcagggaa atcggcaaac ggccgatgga agaattcagg
     1021 aacgtatttt tgcaggcagc ccaagaagca cagacgacaa tagggcagaa ggcggttcaa
     1081 atcgaactgg aaaaattccc cctgaacggc agcccttcgg ccaagcgaat cgcgttttat
     1141 ttcggcgcgc agcggataag ggaagtagag cagaaggagc gggaagacca gcgcaaagag
     1201 caggagcggc aggcgcggcg tatggagcgg gacgacggcc acggcatcgg catgtagaaa
     1261 aaccagccta tcatactggt tggaagacgt attatggtac aaagtcagca aatgcatcga
     1321 aaatatttac ttctcgcata caacgcagat acaattgcag caattcagga agcaaaaatg
     1381 gagcgaaaaa ttgtatcact tctctgtctt cattattaca taaaacactg tgtatttgtg
     1441 cttaacaaca aattggaagt catcggacat tggttttgct tgataattag gggcactcat
     1501 acgctccaat gtattgagca attcagctat gatatttttc ccattacttg atacaaaggg
     1561 tcaagtatcc attcatataa ccgtttattc tcatgcaaaa tatcggcttc tagtatcata
     1621 ccaatttgca atttcttctc cgtgccatat atacgaatat gctgattatc cagttttact
     1681 ttgattaaat aaacaggctc attcacttgt gctgtttgag taaaaatatt gcctaaattt
     1741 gtccattctt gcgcccctag tgccgtttga gcaatagaaa tgacgtgtcc ttctgcgtgt
     1801 ccgaattttt gataaggata tgcttgataa cgcaaaatta ccttgtcatt tggttttaca
     1861 aaaccgattg cgcgactagg gacatataaa ttagccagta attcactttc tttcggcacg
     1921 atactcatca atagtttgtt agtatctacc tgctgaccaa tttccgtgtt aatagaactg
     1981 acatagcctg atatggttgc gcgaatagtt tgttcgttac gttgttgata atccaatatt
     2041 tcttgttgat agacagatac tgcgcgttct aattgactga tttcagtagc ttgctgttca
     2101 ggcagatttt ttagattgct ttgctgtaca gttatttccc gagcaagatt atctgcttct
     2161 cgttggtaag cggctaaacc tgctttcagc tccaataaag caatttcata actatttttt
     2221 tctaattcag aaattgcgtc cattttggct aaataacgct gtttttctaa cattttttca
     2281 gaaatcgcaa tacgtttttc ttgcatgata atttgatttc taacgtgttg catttggttc
     2341 tgaaaacggt gtaccgtgtt ttcttgtgcg gttttttctg ctgcatgaac acgttttttc
     2401 ataataattt cttgttctga tagtgatttt tttaattcag cttcggcgag taatcgtgct
     2461 tgtatgctgc cattattgtc atttcgtgaa gtagataatg taaataacac atcgcctttt
     2521 ttcacaaaat cgccattcat aacgtgttga tgagcaataa cccccatatc ctgcgaatat
     2581 acgcgtacta cccctgttgt cggcaaaagt tgtcctgtta cggtggtttt attggtatag
     2641 ctcccaaaaa ttaaaaaagc aactaaaata gccgctaatg agaccgcgaa aaatgttaaa
     2701 aactgcaagg aaaaaggacg cactaaaaca atttgccctg tccagcgatt ttttttagct
     2761 tggaatactt cggggcgaaa taatgtcatt tcaggctacc ttgtctaaat aaactacgct
     2821 atcagcagta tttaaagttt cctgacgatg cgcgaccatg attttggtaa tgttgagttg
     2881 gcgtagatta tcgttaatca cacgttcatt gttgatgtcc aaatggctac tggcttcatc
     2941 taaaaacaaa atttttggtt cacgatataa agcacgagct agtacgatgc gctgcttttg
     3001 cccaccactc aatgcactcc ccatgtcgcc aatcaatgtt tcatacgcca taggcatagc
     3061 gacaatttca tgatgaatat tcgccatttg agcgcaaaat gcgatttttt ccatattggg
     3121 cgtatcatca aaaaaactga tgttttctgc gattgagcca gtaaataaca catcattttg
     3181 catcacactt gcgctccatg tgcggataaa tgcaggtggt aacgcatgaa tgtcatgccc
     3241 acctaataac acatgaccgc tttcaggttt cagactgcct gtcagaattt gaatgagcgt
     3301 agatttccca cagcctgaat gtccagcaaa cgcaatggct gttccttgtt tgatgattaa
     3361 actgaaattt tgcaaaatgt atggttcatt ttcagcatag ctaaatgata cattttctat
     3421 ttgaatatca aaatgttcta aatttttagg cagactgaaa tcgttgtttt gttcattttc
     3481 cgtttcttct agcacaatat ccgccaagcg ttccgcgtgt aatcctagca ttttgatttg
     3541 tacaaactgg tcaatcaatg aacctgcttt actctcaaac tgacttttgt aggacataaa
     3601 cgccattaac gccccaacgg taaaaccacc gttcaataca gaaaccgcac caaaatacac
     3661 aattaaaata ttagcgatac caaacaaaag gccattcgta aaaccgaaca gcgcggaaag
     3721 tttttgtgtc attaaacctg tgttaatcgt gtcaataaac aagttcagcc aagccgcatg
     3781 gcgttgtgca tttttatcaa ataatttaac agtttgaata ccacgtatgg tttccatgaa
     3841 ataactgctc tgcttggcag catgaacaat attttcttga gttgcatggc gtaatggata
     3901 atatgccacc caacgaatac caatataaat cagcaaaaca accagtacaa ctgtcgttaa
     3961 acttgggcta tacaaataca tcaaccctaa tgtaaatacc gccataatgc tgttgagcac
     4021 caacacaaag aaagtagaag tgagcgtact ttgaatactg tccactgagc caaaacgtga
     4081 aaccacatcg cccaaatgtc gtttagcgaa ataagaaaca ggcaaatcca ttaagcggcg
     4141 taatacatta gctttccatt gaatattcaa agaggtagat aagtacatac ccgcccaagt
     4201 ttgcaacaat gacactaact gccgcacaat catcaacaag ccaaaacaca atgccaatgt
     4261 cattaataaa tcacggtctg ccgatacaat ggcatggtct attacccatt gcataaaaaa
     4321 agggcttgcc aaagcaaaca cctgtaatac tgccgccagc agcagaagct gaaataaaga
     4381 cctttttaat cccaccactc cgcgcaacat cggtagtacg cgaattttac gcgtttcgtg
     4441 tttctcttca aattgcgtgt taggaaatag ttccaacgct acacctgtaa aacagcgcga
     4501 gacttcttcc atacgcactt tttgccaacc cgaagcaggg ttcataatca taatgtgatt
     4561 gcgatgaact tcatgcaaca ctacaaaatg atttaaatcc caatgtaaaa tacagggtaa
     4621 gcgcaaattt accaattcat ctaaatctaa ccgaacagct cgagcggtta aattcatgct
     4681 attagcaaac cgtaccaaat ccgccaatgt tgcgccttta agcgacaaag aaaaacgcgc
     4741 ccttaaatgg cgcaaatccg tataaaaacc atgaaacccc aacaccgata ccaaacacgc
     4801 taatccacat tcggctgctt ccgtttgcaa aataacgggc aaacggcgcg agaaactgaa
     4861 tgataggcga gagagatatg ttggcattag agttcctatt ttatttttta tttaaaaaaa
     4921 ttattattac cgataataat actgataata aaaaaaataa aactattttc atttgattac
     4981 gacttaatgg aggctaatgt attaatccgt aatgtacaga taagccaacc aaaaaaatca
     5041 aaacaagaaa gaaagccatg agtacttggt aaggattcat caatgctatc cctataattt
     5101 tgtttaatac cctgttccat caccgtaatc attacctgat cgatcattat ccaaaatgct
     5161 ttcaatacct cgtccgatca caccaccaat ggcagatcca gctcctgccc caattacaga
     5221 accaacctta ccaccatatt gttgtccagc tgcaacacca actatagtac cacctggacc
     5281 aaatggagag cctagaattc ccccgatggt tccaccacct gctttccctg caatatatcc
     5341 agtaccacca ccaatagcac ctccctctcc tgtagtgaaa tcagcaatgg gaccagcacc
     5401 actaactaat tcaagatcca caatagagag ttcatacatt tttatttacc tcaaaataaa
     5461 atttaataat aaaaaagaaa tgagttagac agaatagtat cactataaaa atgatagaat
     5521 aaatattcat attaaaataa aaattttcct gtctaaatag agttattaag gcaatataca
     5581 aaataaccat aaaataaaaa gtgaatattt ttacaaattc tattttattt tgatttttat
     5641 ttattcctcg tgcaaaaatt accattttat aaatcaaaag caatgttgaa attataaacg
     5701 aaaatgctat taatatagaa atatgatttg caaaaggtat agttgataaa ttaaatctaa
     5761 ctttactgta tacaatgtat gcgttcaata aaataaatga tattgaggat atattgatta
     5821 aaatttttgt gttatttaat aaaacaccca ttgattacct cacgatttca ataacatcta
     5881 ataaacataa ctccctaact aatttcttaa tattttcttg ttcatcaaca ctaaacaacg
     5941 ataatatttc agctagggat atcatgtaaa gcaagaaaga aagccatgag tacttggtaa
     6001 gcattcatca atgctattcc tataattttg tttaataccc tgttccatcg ccataatcat
     6061 taccagatgc atcattaaaa ttttggtcta gtcctgttaa tttatcaact cctgaactta
     6121 accaatcgcc taatggcgtt tcatgatata aataagtacc tgtattccag cctgctatgc
     6181 ctgccaaacc aattaatcca cctcgagcag cacttcttgc tatcatatcc ccagttgcag
     6241 tagctgaaac agctctccct ccacctaccg ctgcactacc tataccaata gcatcaagag
     6301 gattaatgta agctggttca gcaccacctg aaactatttt caggtcagaa atatttaatt
     6361 ctttcattgc ttttcctttt taacaatctt atttttcaat atgattgaat agtacgctca
     6421 tgccaatacc aatcataata ctaaatacta tattaatgta atctccgtca taattaggat
     6481 gaagacgcca ccctcctaat tctgataatt tcataaaaat atatattaca atagaatatc
     6541 caattgcatg taaaaatgtt ttcatatatt ttatatccta aaatattaaa tcaaatatta
     6601 aatccatctc atcttataat ttcaattata tctaactctc ctagccgcca aactaatttc
     6661 ttaatatttt cttcttcatc aacactaaaa aacaataata tttcagctag ggatatcatg
     6721 tatttatcaa ccaaaatcgt taaaaatttc atactacttt catcaacatt tagcttaata
     6781 ccattcacga taagaaatcc ttgtggaaga gttgagcaat cattactatt taatcttaaa
     6841 aagcaatgct caggtaatgt ctgattatga gcatttccaa acaaatccat attgaatgct
     6901 gtattggtgc gctgatttcc caaaaaatct tgcataaatg cttgataatt ttttgggtta
     6961 ttcatcattt cagtaactgc ttgagctgaa tcatctagat ttttttggtc atgttcccag
     7021 cctatgaaat tttgacgaat actctggata tcaggtattt ttttcatcaa ccattccata
     7081 tagttataac cgtttggcgg aaatgtacca atagctaaat ggaatgtttc acaattcata
     7141 ggcataggat tatgccacca accacgtgga atatatagaa tatctcctgc ttctaaaatg
     7201 acttccatat ccactgtttt agatggagta atgtgcggca tatcttttgc ttgctgcata
     7261 tacaacggca tatcaaaatt tggggcagaa attgtccaat gttttttgcc aatcaattgt
     7321 actgcaaata catctcgagt atcccaatga ttacgatatg atgatgagct accaaacgct
     7381 aaataaccac taacaacagt ttgtgcttgt gcaaattgag caacttgttt agcaatagta
     7441 tcaacgaatg gctcattatc aatacggtta taaaccattg tcgcgccatc ttgcaaatac
     7501 tgatacacgg ctgttttatt aaatcgatga cgtatcctac ccacgtcatt gaatgattca
     7561 acataggctt ctttggggat tatttcccct ttgcgaaatt taaattgcca atcagtaggg
     7621 tctgcacgtt gatacaactc attaatttct ttccatgaga tagaacttac gtctaaagct
     7681 tttttaaaaa taaacggctt tttatataaa taattttcat taaattcttt atattccatt
     7741 gaaaagttaa tatgcaaaaa taatccttat tttcattatt aataatgaaa attaagttta
     7801 cttaactagt gtttatatgt caagttttgc aatgaaaaga cgttctgttt ttggaagaag
     7861 gtattttgtt atttttagat aaagttggat attgctttga cattatggtc aaatacattc
     7921 tgccgtgtgt tattatttat tcaataaatc tgaaacgagt ttattgagca cgacgcgaat
     7981 agttacacca ttctcagccg cgtatttctt caattctcta tgcagagatt cagacatatc
     8041 aaaactgatt ctcttgattt cttcttgctt gatcacatca tcaatagata agtttgcttt
     8101 gtttgcacta ggacgatctg cttttaatcc gttcataatt atttcctaca atatttaaaa
     8161 atttaaaata atatttcatt aaatatgatt atctatattt atcaaattta ttatctcatt
     8221 cgcaagtgat tgaatttcct tagctgcttc tccatttggt tctttatcca gtacagtgct
     8281 acctgtgata gcagatgttg gataaataat acgttgcaca atcacggttg ataaaacagg
     8341 taactcatat tcttctaacg ctgctctaat ttctccgcca atctttgttc ctttaatagt
     8401 tcggttaatt aaaaaagcag cctttaaatt tccatctatg agttttatcc gctgcttgat
     8461 taaattaacc aaatcagcgg ttgcccaaat atcgtatggc gacggttgaa cagggattaa
     8521 aactaaatca gaggctttaa tcgctgatat ggctaaatcg gctgtttgtg gtgcgccatc
     8581 aataataaca aaatcttttt aaacaacgct ttttaaatca cgatcaattg tcgggctgtc
     8641 aataccaatc acaggaacag gttgctcttc gtgaacagca gcccagtccc tactgcttcc
     8701 ttgtggatcg ctatcaacca ataagatgct atttcctgct aattgcaagg cgcgtgccaa
     8761 gtgagttgcg actgtggttt taccacttcc acccttttga tttaatacgg aaataacttt
     8821 catgttagct cctttgaata tctacgctca ttatgctttt aattaaataa tattttaatc
     8881 tattatttaa ataaataaaa gaatatttac attttgtttt aaattttagc aagaaaagct
     8941 gttaaagaga ttactgtatc aaactagact tttttgagat atgtggcaga aaatgagttg
     9001 atattgcgtt aaagtttaaa ttgagacctt tgcaaaaaag cccttccccc aacagccgaa
     9061 acccaaacac aggttttcgt ctatttccgc tcctaattac tcctgatttt acccaaatgc
     9121 ccccttaatc ctccccggac gccc
//
LOCUS       DAWGJV010000078         9049 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWGJV010000078 DAWGJV010000000
VERSION     DAWGJV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.078"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(175..867)
                     /locus_tag="WHQ94_001315"
     CDS             complement(175..867)
                     /locus_tag="WHQ94_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ2128585.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILHPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WHQ94_001316"
     CDS             complement(975..2168)
                     /locus_tag="WHQ94_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ2128586.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WHQ94_001317"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WHQ94_001317"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ2128587.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WHQ94_001318"
     CDS             complement(3021..3950)
                     /locus_tag="WHQ94_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ2128588.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WHQ94_001319"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WHQ94_001319"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ2128589.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WHQ94_001320"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WHQ94_001320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ2128590.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGVGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7342..7881
                     /locus_tag="WHQ94_001321"
     CDS             7342..7881
                     /locus_tag="WHQ94_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ2128591.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="WHQ94_001322"
     CDS             complement(8071..8286)
                     /locus_tag="WHQ94_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128592.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8294..8497)
                     /locus_tag="WHQ94_001323"
     CDS             complement(8294..8497)
                     /locus_tag="WHQ94_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128593.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8410..8768)
                     /locus_tag="WHQ94_001324"
                     /pseudo
     CDS             complement(8410..8768)
                     /locus_tag="WHQ94_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
     gene            8821..>9049
                     /locus_tag="WHQ94_001325"
     CDS             8821..>9049
                     /locus_tag="WHQ94_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2128594.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWN"
BASE COUNT         2275 a         2474 c         2234 g         2066 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccat gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gtgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaagccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtacttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccag
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggtgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catctctatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga accctgaaca actttccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcaggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgtatcgc
     4921 atggcttttg caaaccggtc aggcaagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgact cggcgcgatg attcccgtcg acgtccgcgc
     5041 tatgtggctc ggcacgttcc acggcctctg ccaccgcttt ctacggctgc accaccgcga
     5101 tgccggtttg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtcttgagtg cgcccgatcc
     5281 gcacacaagc cgcatgattg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaactg tccctgtccg aaatcatcgt cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgtctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttc gaaatcctgc ctgaaaacat
     6361 ttcagacgat cccgccttcc ccatcctcgc tttcttgagc aacgccaccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgtgg gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggtatggaa gaaggccgtt tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgccaaa ccgccgcatc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aatttggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggacaccaa
     6961 gtttgcgaaa ttggaagaga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacgca gagaaattcg
     7561 tgggcggaaa atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggctggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gcgtgcggca aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcgaaataac ggataatcgc ttgaatccgt aaggaaaacg gtttggtgga acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaacct
     8581 tctttattac aatgatggga aatccattta ggagaacaaa tgcaaagtga aaaaatagat
     8641 gagccttgtt ctccttcgat tccgatatcc aaatctatcc atctatggaa attatctgga
     8701 atttcggggg taaatttttc aaaatcaata tcatataaat ttatgctttt taaatccaat
     8761 ttaatcatta gggctgtcct agataaatag ggaaattcaa attaagttag aattatccct
     8821 atgagaaaaa gtcgtctaag ccggtataaa caaaataaac tcattgagct atttgtcgca
     8881 ggtgtaactg caagaacagc aacagagcct gacagcattg tttatacgga ttgttatcgt
     8941 agctatgatg tattagatgt gagcgaattt agccatttcc gtatcaatca cagcacacat
     9001 tttgctgaac gacaaaacca tatcaatgga attgagaatt tttggaacc
//
LOCUS       DAWGJV010000079         9026 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWGJV010000079 DAWGJV010000000
VERSION     DAWGJV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.079"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="WHQ94_001326"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="WHQ94_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ2128595.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVDDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /locus_tag="WHQ94_001327"
     CDS             996..1475
                     /locus_tag="WHQ94_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128596.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALR
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /locus_tag="WHQ94_001328"
     CDS             1477..2475
                     /locus_tag="WHQ94_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ2128597.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAVLKQGQGVQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="WHQ94_001329"
     CDS             2596..3015
                     /locus_tag="WHQ94_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128598.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="WHQ94_001330"
     CDS             3090..3605
                     /locus_tag="WHQ94_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ2128599.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="WHQ94_001331"
     CDS             complement(3689..4573)
                     /locus_tag="WHQ94_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ2128600.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="WHQ94_001332"
     CDS             complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="WHQ94_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ2128601.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="WHQ94_001333"
     CDS             complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="WHQ94_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ2128602.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7791..8159)
                     /locus_tag="WHQ94_001334"
     CDS             complement(7791..8159)
                     /locus_tag="WHQ94_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ2128603.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8059..8517)
                     /locus_tag="WHQ94_001335"
                     /pseudo
     CDS             complement(8059..8517)
                     /locus_tag="WHQ94_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8713..8949
                     /locus_tag="WHQ94_001336"
                     /pseudo
     CDS             8713..8949
                     /locus_tag="WHQ94_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT         2000 a         2249 c         2640 g         2137 t
ORIGIN      
        1 taagggcatc attcccgcac cggcaagaat ccgaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca aacatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcgacgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agtaccgtcg
      421 tcgtcaatgc tgccgaagcg ttgggaggca cacccgatgc ggacgcttcc gtagacgaca
      481 cgggcaaagt tgcctgtgcc gtgatgaccg cagactgcct gcctgttttg ttttgcgaca
      541 gggcgggtac gacggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgccgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ccgccatcag tgcggatgcg tttgaagtcg gacaggacgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggtgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gcgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggatactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcggttt cctttggaaa
     1141 cagcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc cttcgattgg aacgaactgt tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ccaacggaaa acccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttgctgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggccttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcgaggcag acgcattggc actgtttgcc
     1981 gagcgcgtgg aaggcaattt gttggcggcg cgtcaggaaa tcgacaagct cgcgctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgttgc caatgttgcc
     2101 cgctttgacg tttttcagct tgccggcgca tggatgaagg gcgatgtccc gcgcgtatgc
     2161 aggttgttgg acggattgca ggaggaaggc gaagaaccgg tactgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gattcggctt gccgctgtcc tgaaacaggg gcagggtgtc
     2281 caatccgtac gcaacagtct cagactttgg ggcgacaagc agacgctcgc gccgcttgcg
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgcgc ccaaatcgac
     2401 cgaatcatca aaggcgcgga agacggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccccaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatta accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgccg ccgtcatcgt cgccgccgct gcgggcggct ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccaca tgagacatat ctgcatgaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattccg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgtttgc cggtattgcc gccatttttc tgaatcccgt ttctattgcg gtttctgcat
     3241 tggtaacgag tatcctgata atgggatcat ggtggcttta tttccgtgcc aactggcacg
     3301 ggcagggaca gaccttggcg atgaggacgt ggaaaatcgg tttgtgcgac cttaacggca
     3361 tacagccgtc tctacactta ctccgcctgc gctttatttg ggcgtgcata ttcatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgccctg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggcattt gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct
     3721 gttcgatcag gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcggcaa ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccatg ctttcgaggc
     4081 gttgtaacag gtgctcgccc aattttttcc cttcgcgccg gcgtgcggcg gtaaagtcct
     4141 tcagtgccga atcggtcaaa tcggcaataa cctttgccaa tgcgtccgca ttttcccttt
     4201 gacttgccaa tacgcccgga aagcgcaaaa tatcggcaac gcccagtttt gccaaatcat
     4261 aatgcttgcg gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt
     4321 ccaaagactg attgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gctgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaaccaag accagtacgg
     4741 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
     4801 cggttccgta cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc
     4861 cactatatca attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat
     4921 gtctgctttt tgatttcggc aattgcaacg gcgtggacgg ataaaatcgt gtacagcacg
     4981 acgcacaaac cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta
     5041 ttttattgcg tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct
     5101 gcatggcttt tgcttcaatc tggcggatgc gctcggcaga gacgccgtat tctgcagcaa
     5161 gctggtgcag cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc
     5221 tcctgtcatc cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg
     5281 cctgtttcga tagttgccgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg
     5341 cgaaactgtc ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc
     5401 gctgttccat ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg
     5461 cctctttggg agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct
     5521 tgcgttgcgg tttggtggtg gcaacgcgaa ccaaacgcca gtttctcaaa ataaactcgt
     5581 ggatttcggc tttaatccag tgtacggcaa atgaaaacag acgcgcgcct ctaccgggct
     5641 cgtagcgttt gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat
     5701 tcagcccgta gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga
     5761 tgagttgttt ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt
     5821 cttcctggga cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt
     5881 tgccgctttg gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc
     5941 tgaaactgcg tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta
     6001 ttttgtatgt ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg
     6061 caggtttgat tgattttgta aacctgatgg ctcaattcga tttcagaatt atattacata
     6121 cgtaattgta tgtaaatagc cgttttgaaa aaagacagcc cgtccggacg ggctgtgcag
     6181 gtatcagtgt tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc
     6241 catcaaccac catgaactgc cggttttcat atagggcgtt tcgccgacat agcctttgat
     6301 gtgtccttcc aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg
     6361 ggagatgatg gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat
     6421 agcccgcgcc tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc
     6481 catattgctg gcattggcaa gcagtgtggc atctttggcg gcggcaatca gttcgtcgcc
     6541 gaatccgtct tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga
     6601 ttgcttaccg ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg
     6661 cgtggtcagg aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta
     6721 gtaggggatg ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc
     6781 gtaaccgttg ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt
     6841 ttgcgcctgt tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg
     6901 gatggcggtt tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata
     6961 tttctgtatg gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc
     7021 ttgaagcagg gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc
     7081 ggtgtagcct gcggcaagca gggcggcaat caggataatt ggaagcaggc gtttgcccga
     7141 acgtgcggtg ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt
     7201 aaccatgtgg atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg
     7261 ggagtagccg attgcgcccc agccgaatcc ggtcaggaaa cgttcgcggg caaactcggt
     7321 cagtgtccac aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt
     7381 tttccacagc cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt
     7441 cagcggtacg gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca
     7501 gtagaactgt gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg
     7561 cggacgcagt tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg
     7621 gtagtagggt gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta
     7681 gagggcgggg tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg
     7741 aagataccgt ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg
     7801 ccgaaacgat tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg
     7861 acaacgaggt tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa
     7921 tcgtgcagaa ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc
     7981 gtgccgacca cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc
     8041 aaactgcgcg aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg
     8101 acgatgacgg caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt
     8161 acgagcatgc gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa
     8221 gcacagataa atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga
     8281 aagcagcagg gccgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga
     8341 ccaaacaatc ggcaactcgc gttctgccga tacgcccgta acacggttgg cattgggtgc
     8401 caaatcgtcg ccgaccacgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac
     8461 atcgtccaaa acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa
     8521 agaatcctga aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt
     8581 ttcaggcggc atgaaacagg aaaatgccgt ctgaacgctg tggcggacgt gaagtaaagt
     8641 ttcgtgaaaa gaaaatgccg tgttacagtc tttcgatttt aatttaatga attttaaggg
     8701 agaatcgtta gcgtggattg gatgggcagt ctgttcctgc cgggtggcgc actgttgttt
     8761 ctgagcgtgg tttcgaccac tttacccgca cgtttgggaa tgcctttgct gctggtttct
     8821 cccgccggcg tgttggacgg ggcggcggaa gccttggcga ttgcggcgtt cctgatgctg
     8881 gtcgcgcgtc cgccggcagt gttcggcggt ttgtggaaat tcaattacag cctgcgtgaa
     8941 aaggcgtata gccgaatcga aatgcagtcc gacaccgtgc ttcaggcggg gatttggcgt
     9001 ggtacatcct gcccgacggc aaggtc
//
LOCUS       DAWGJV010000080         8948 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWGJV010000080 DAWGJV010000000
VERSION     DAWGJV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.080"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            130..972
                     /locus_tag="WHQ94_001337"
     CDS             130..972
                     /locus_tag="WHQ94_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ2128604.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            1002..1466
                     /gene="nrdR"
                     /locus_tag="WHQ94_001338"
     CDS             1002..1466
                     /gene="nrdR"
                     /locus_tag="WHQ94_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ2128605.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGLRD
                     IPSAELADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1498..2607
                     /gene="ribD"
                     /locus_tag="WHQ94_001339"
     CDS             1498..2607
                     /gene="ribD"
                     /locus_tag="WHQ94_001339"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ2128606.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTTLSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            2802..4217
                     /locus_tag="WHQ94_001340"
     CDS             2802..4217
                     /locus_tag="WHQ94_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ2128607.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLPLTVG
                     LLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPIA
                     LSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRAI
                     EENAPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPLF
                     CTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFWLF
                     FVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAGCI
                     LRHRKDLHKLFHYLKKQGFPL"
     gene            4214..4822
                     /locus_tag="WHQ94_001341"
                     /pseudo
     CDS             4214..4822
                     /locus_tag="WHQ94_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            4761..5285
                     /locus_tag="WHQ94_001342"
     CDS             4761..5285
                     /locus_tag="WHQ94_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2128608.1"
                     /translation="MSRLDTIRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDNLKAQ
                     AERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYDTPVV
                     TYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKRLHAHVAALCS
                     DDEIYRTTMAAYTM"
     gene            5305..6468
                     /gene="pglH"
                     /locus_tag="WHQ94_001343"
     CDS             5305..6468
                     /gene="pglH"
                     /locus_tag="WHQ94_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ2128609.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKIPHRLLPFA
                     RRADRAYRCADALVAVSQTYLDRAKEANPNVPGETVYIGTDFAAIAPPRFRSKTVRLF
                     YLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYLPY
                     SEMMSIAKACDIAVNAIHNHAMQSVTNKLSDYMALQKPILNSQTNAEVLSLLNLLPHE
                     NYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKIVNLIERLANE"
     gene            6461..8038
                     /locus_tag="WHQ94_001344"
     CDS             6461..8038
                     /locus_tag="WHQ94_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ2128610.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            8025..8711
                     /locus_tag="WHQ94_001345"
     CDS             8025..8711
                     /locus_tag="WHQ94_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2128611.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
BASE COUNT         2004 a         2842 c         2212 g         1890 t
ORIGIN      
        1 aaccttaagt ttccgtcttt gttttctgtt ttcgcgggaa taatggggaa caaaccgtat
       61 ttcagacggc attttcagtt cggggtataa tccgaatact tgcgaccatc tgaatcattg
      121 ggacaaacta tgtgtcaact gctgggcatg aactgcaata cgccgaccga tattatgttt
      181 tcctttgaag gcttccgccg gcgcggcggc attaccgacc atcatgccga cggtttcggc
      241 atcggctttt tcgaaggcaa aggcgtgcgc ctgttccatg acgacaaacc cagtgccaat
      301 tcgcccgtcg ccgacctcgt gcgcgcctac cagattaaat cggaaaacgt cgtcgcccat
      361 atccgcaaag catcgcaagg acaaacctcg ctggcgaaca cccatccctt tatgcgcgaa
      421 atgtggggtg gctactggct gtttgcccac aacgggcatt tgattgattt tttccccgaa
      481 cagggcgaat ttttccaccc cgtcggcaca accgattccg aacgcgcgtt ctgccacatc
      541 ctcaaccgcc tgcgcacccg ctttgccgcc cgtcccgacg acgacacgct gtttgacgcg
      601 attgcggggc tgacgcacga aatccgcaag ttcgggctgt ttaactttat gctttcagac
      661 ggcattgccc tgtttgccca cgccagcaca ctgctgcact acatcgtccg ccaagccccg
      721 ttcggcaagg cgcgcctgct cgacgacgac gtgatggtcg attttgccga agtaaccacg
      781 ccctccgacc gcgtcgccgt tatcgccacc ctgccgctga cccgcgacga atcatggtcc
      841 caacttgccg tggacgaact ggtcatgttc cgcgaaggca acatcgtccg acacgaccgt
      901 cccgaaaacc ccgtttatat gagtgtcgaa gaaggtttgg aaaccgcgcg cgccgccggc
      961 gtcgccgtct gaacttcaga cggcatagga ggacgaaccc gatgaaatgc ccgttttgcg
     1021 cccaccccga cacccgcgtt gccgattcgc gcctgatgga cgaacgcaac gccgtccgcc
     1081 gccgccgata ctgccccaac tgcggcaaac gcttcggcac gctcgaaacc gccgaactca
     1141 aaatgcccgc cgtcatcggt ccggacaaaa aacgttcgcc ctttaatgca caacgcctcc
     1201 gcaacgacct gaccgccgcc gcccgaaaat ccgccctgac acccgaacag atcgacgaaa
     1261 ccgtccgcct gacggaacac aggctctaca cttcgggtct tcgcgacatc ccctctgccg
     1321 aacttgccga catggtgcta aaagagctgc ttgagcacaa cacggaagcc gccgtccgtt
     1381 tcgccgccct gcacaaacgc ttcgacaatc cggcagactt tgcctcgtgg ctggcgcaag
     1441 ccgtcaaaac aggcggcaaa gcctgattcc cccaacccat actgatacgg tatcccgatg
     1501 ttttccgaaa cagatatttc tatgatgcaa aacgccctca aacttgccgc tttggggcgt
     1561 ttttccactt cgcccaatcc gcgcgtcggc tgcgttatcg cacacggcag ccaaattgtc
     1621 gggcagggct tccacgtcaa agcgggcgaa ccccatgccg aagtccacgc cctgcgtcag
     1681 gcgggcgaaa tggcacaagg cgcgaccgcc tttgttaccc tcgaaccgtg cagccattac
     1741 ggacgcacac cgccctgcgc cgaagctctg ctccgttccg gcgtaacacg cgtcgtcgcc
     1801 gccatgcgcg accccaaccc gctggttgca ggcaaagggc ttgccctgct cgaagcagca
     1861 ggcatcaaga cggaatgcgg tttactcgaa catcaggcaa gggaactcaa ccgaggcttc
     1921 ctgtcgcgca tcgaacgccg ccgccccttt gtccgcctca aatgcgccgt ttcgctggac
     1981 ggcaaaacca ccctttcaga cggcagcagc ttttggatta ccggcgaaga cgcgcgtgcc
     2041 gacgtacagg ttttgcgtgc cgaaagctgc gcggtgctga ccggcatcgg cacggtgttg
     2101 gcggacaatc cccggctcaa cgtccgcgct tttccaactt tgcgccaacc cgcacgcatc
     2161 gttttagaca gccgcctgcg cctgcccttg gacagccacc ttctgaccga cggacaatct
     2221 ccaacctaca tcgccacact cgaacgcaac gaagacagac tgcaccccta tcgggaacac
     2281 gcacacatcc gcatcctgat gccgtctgaa acggcaggcg gcaaaatcga cctgaaccac
     2341 ctgatgcgcc tccttgccga cgaaggtttc ggagaaatca tggtcgaagc aggctccgaa
     2401 ctcacatccg catttctggc agaagatctg gcggacgaaa tcgtgctgta ccgttcgccc
     2461 aaaatcctcg gcagcggcaa agacctgctt tccctgcccg aaaaccgcgc cgccctttcc
     2521 gcaccgccct tgtggacacc cgtttcaagc gaaatcctcg gacacgacat caaaaccgtg
     2581 ttccggaaaa acgataacgc cttttaaagg gtttgcgccg tttcactata taataacgcc
     2641 gataaaaaac ggccccgttc agaccgccgc ccgcccggaa aagcgcaacc cggactgccg
     2701 caccccgccg gcagccgcaa cggactgaaa gccgtcaaat tccgatcaag aaaggcttca
     2761 gacggcacag gcagcatccc gccgccgccc ggacatcaaa aatggacaca aaagaaatcc
     2821 tcggctacgc ggcaggctcg atcggcagcg cggttttagc cgtcatcatc ctgccgctgc
     2881 tgtcgtggta tttccccgcc gacgacatcg ggcgcatcgt gctgatgcag acggcggcgg
     2941 gactgacggt gtcggtattg tgcctcgggc tggatcaggc atacgtccgc gaatactatg
     3001 ccgccgccga caaagacacc ttgttcaaaa ccctgttcct gccgccgctg ctgtctgccg
     3061 ccgcgatagc cgccctgctg ctttcccgcc cgtccctgcc gtctgaaatc ctgttttcgc
     3121 tcgacgatgc cgccgccggc atcgggctgg tgctgtttga actgagcttc ctgcccatcc
     3181 gctttctctt actggttttg cgtatggaag gacgcgccct tgccttttcg tccgcgcaac
     3241 tcgtgtccaa gctcgccatc ctgctgccgc tgacggtcgg gctgctgcac tttccggcga
     3301 acaccgccgt cctgaccgcc gtttacgcgc tggcaaacct tgccgccgcc gcctttttgc
     3361 tgtttcaaaa ccgatgccgt ctgaaggccg tccggcgcgc gccgttttca tccgccgtcc
     3421 tgcatcgcgg cctgcgctac ggcataccga tcgcactaag cagcatcgcc tattgggggc
     3481 tggcatccgc cgaccgtttg ttcctgaaaa aatatgccgg cctagaacag ctcggcgttt
     3541 attcgatggg tatttcgttc ggcggggcgg cattattgtt ccaaagcatc ttttcaacgg
     3601 tctggacacc gtatattttc cgcgcaatcg aagaaaacgc cccgcccgcc cgcctctcgg
     3661 caacggcaga atccgcctcc gccctgcttg ccgccgccgt ctgcctgacc ggcattttct
     3721 cgcccctcgc ctccctcctg ctgccggaaa actacgccgc cgtccggttt atcgtcgtat
     3781 cgtgtatgct gccgccgctg ttttgcacgc tggtagaaat cagcggcatc ggtttgaacg
     3841 tcgtccgaaa aacacgcccg atcgcgctcg ccaccttggg cgcgctggcg gcaaacctgc
     3901 tgctgctggg gcttgccgta ccgtccggcg gcgcgcgcgg cgcggcggtt gcctgtgccg
     3961 cctcattctg gttgtttttt gttttcaaga cagaaagctc ctgtcgcctg tggcagccgc
     4021 tcaaacgcct gccgctttat atgcacacat tgttctgcct ggcctcctcg gcggcctaca
     4081 cctgcttcgg cacaccggca aactaccccc tgtttgccgg cgtatgggcg gcatatctgg
     4141 caggctgcat cctgcgccac cggaaagatt tgcacaaact gtttcattat ttgaaaaaac
     4201 aaggtttccc attatgaaaa tcgttttgac cacatctatg gcaggcttgg gcggcacgga
     4261 aaccgccacc gtccgcctcg ggcggctgct caaacggcgc gggcacgaca tcatccttgc
     4321 ttcttcggac gggccttttg tcggagaggc gcaagcatcg ggcatccgtt ggcagccggt
     4381 tgatttttac cggggcgggc ttgccggcta cctcaaaagc acctttgcct acgcccggat
     4441 gctgcgccgc gaacaacccg acatcatcga ctgccaaatg gcgcgcgtcg ttccagcctg
     4501 cgcccttgcc gccaaaatcg tttcgccgaa aaccaaaatc atctaccact cgcacggttt
     4561 ggacgcggca acctacccca aaatcgccaa actcttcgac aagctgggcg tgtacatcat
     4621 cggtaactgc aaacacgaac gcgaaaaact catccgccac ggtttccccg ccgaccggat
     4681 tgtctacgcc tacaacgcac tccccccccc cggaatttcc tttccggaaa accgaaaaag
     4741 aatgcgccgt actcggcacg ctgtcgcgtc tggacaccat ccgcgccgtg catctgatgc
     4801 tggacatttt caaaaaaatg gtcgaccgcg atatgcccgt acgcctgaac gtggcgggca
     4861 taggcgaaga aatggacaat ttgaaagcgc aggcggaacg tctgggcata gacggcaaag
     4921 ttaccttcct cggcggcgtg cgcgatttga ccgcctactt caaagacgtg gatattttgg
     4981 tgaacacgcc ccactgcatc ggcgaccacg gcgcaggcgt gggcaacaac atcttagaag
     5041 ccggacttta cgacacaccc gtcgttacct acgatatggc gggcatttcc gaaatggtcg
     5101 tcaacgggga aacgggttac tgcatcccct tcggcgacga agaagccttt atcgccgccg
     5161 tagacacgct cgtcagacaa ccggagttgc gcgaagcaat gggcaaaagg ctgcacgcac
     5221 acgtcgccgc actctgttcc gacgacgaaa tctaccgtac caccatggcg gcctatacca
     5281 tgtaatgccg tctgaaagcc gccgatgaac atcaccatag tcgcccctta ttgctcgctg
     5341 ccgtccgaac cgtatttcaa ccgcttttgg tatctggcag aacgattgtc gcaatcgcac
     5401 gacgtgttgc tgattaccag ccgtttccgc cactacgaca aatcattcag acggcatgaa
     5461 gatgctgccg ccacttcaaa cggcaggctg cgcgtcaagc tgctggacga acctggctac
     5521 cgaaaaaacg tctccctcgc ccgcgtcgcc agccaccgcg tattcgtccg caatttggca
     5581 cgctggctgc acagcccgca ggcggcggaa caggacatcg tctattctgc ctatccgctg
     5641 atggcgacca acctgctgtt gggcaaacac aaagcgcgtt tgggctacaa actgattgtc
     5701 gatgtgcagg acgtatggcc ggagtccttc tcatcggtcg tgccgttttt gaaaaaaatc
     5761 ccgcaccgcc tgctgccctt tgcccgtcgc gccgaccgtg cctaccgttg cgccgacgca
     5821 ctcgttgccg tctcgcaaac ctacctcgac cgtgccaaag aagccaatcc gaacgtaccc
     5881 ggcgaaaccg tctatatcgg tacggatttt gccgccatcg cccccccgcg attccgctcc
     5941 aaaaccgtcc gccttttcta cttgggcacg ctcagttaca gctacgacgt ggaaaccgtg
     6001 tgcaaaggcg ttcggaaact tttagacgac ggcgaaaacg tcgaattgca cattatgggc
     6061 ggaggacccg atttggagaa actcaaacaa tatgaaaatc gcgccatcaa gttttacggt
     6121 tatttaccct attctgaaat gatgtcgatt gccaaagcgt gcgacattgc cgtcaacgcc
     6181 atccataacc acgccatgca gtcggttacc aacaaactct ccgactatat ggctttgcaa
     6241 aaaccgattt taaacagcca gaccaatgcc gaagtcctta gcctcttgaa cctgctgccg
     6301 cacgaaaact accgttccgg cgacgtggac agcttcgttc aagccgccaa agatattttg
     6361 aaacgcaaaa acgaccccgt tcagtccgat gaaatcgtcc gccgcttcag gcgcgacatt
     6421 tcctatcgga aaatcgtcaa cctgattgaa agattggcaa atgagtaaag ccgtcaaacg
     6481 cctgttcgac atcatcgcat ccgcatcggg gctgattgtc ctctcacccg ttttcctgat
     6541 tttggcgtac ctcatccgca agaatctggg ttcgcccgtc ttcttcattc aagaacgccc
     6601 cggaaaggac ggaaaacctt ttaaaatggt caaattccgt tccatgcgcg acgcgcttga
     6661 ttcagacggc attccgctgc ccgacagcga gcggctcaca cccttcggca aaaaactgcg
     6721 tgccgctagt ttggacgaac tgcctgaatt atggaatgtc ttaaaaggcg acatgagcct
     6781 ggtcggcccc cgcccgctgc tgatgcaata tctgccgctg tacgacaact tccaaaaccg
     6841 ccgccacgaa atgaaacccg gcattaccgg ctgggcgcag gtcaacgggc gcaacgcgct
     6901 ttcgtgggac gaaaaattcg cctgcgatgt ttggtatatc gaccattaca gtttttggct
     6961 ggatatgaaa atcctgtttt tgacagtcag aaaagtcttt atcaaagaag ggatttcggc
     7021 ggaaggggaa gccactatgc cttatttcac tggaaatcaa actaatgaaa aaaataatat
     7081 actgattctg tcggcaggtc gccgtgtcga attggtgcag gacttccaaa ccgaagccgc
     7141 acgtttttca gacggcatca gcgtttttgc cactgacctc aacccccgca tgtcttctgc
     7201 ctgccatgtt tcggacggtt catttgaagt gccgccgatt agtaccgatt catatatcga
     7261 cagcattttc aatctggcgg tacgcgaaaa tatcggttta atcatcccaa ccatagacac
     7321 cgaactgcaa aagctggcag acgagcgcga acgctttgaa gcggcaagtg tccacatcgt
     7381 cgtttccgat tcggacttca tcgcacaatg ccgcgacaaa cggaaaaccg ccaccttgtt
     7441 tgcccgctac ggcatacgct cacccgaaat ttacgaccgc aagaagttgg ttttcccttg
     7501 cttcgccaag ccttatgacg gcagccgcgc catcggtgca aaacggattg acacacctgc
     7561 cgacctgaca ccggaagtct tggcagacac caaattaatg ttctgtcagt ttatcgacat
     7621 cgaaaataaa ttttccgagt ttaccgtcga tatgtattac gaccgtcaat gccgtctgaa
     7681 atgcgccatc ccgcgaaaac gcttggaagt gcgctccggc gaaatcagca aaggcgttac
     7741 ctgcaaaaac agcctctacc aaacgctgct ggaaaaaatg gcggtattgc agggcgcgcg
     7801 cggctgtatc acggcgcagt tcttctacaa cgaagaaacc ggcgaattct acggcgtgga
     7861 aatcaacccc cgcttcggcg gtggcttccc gctgacgtat gccgcaggcg gcaattatcc
     7921 gagccggttg atgcgcgaat acctcggcga cgaagaaatc ccgttttcag acgactggga
     7981 aaacaacctc attatgttgc gctacgatgc caaggtcttg gttcatgaaa acgattaatc
     8041 ctgaaaccac cgtcatcgtc ttcgacttgg acgataccct gtattcggaa tacgaataca
     8101 agctctccgg tatccgttcg gtcatcgata ccgttgccgc actgtatccc gattggaatt
     8161 cagacaacct atggcgcaac atcgaccccg acggcaaaga ttggctggac aaattgtgcc
     8221 gccactgtgg ttttaatgaa tcggaaaaac aggttctttt gtggcaatac cggctgcacc
     8281 gcccgacgct gactccgtat gctccgccgg atttcctgtc cgaactgacc gcgcccttcg
     8341 ccgcacgcgc gctgattacc gacggcagaa gcctgaccca acgcctgaag ctggaagcct
     8401 tgggtctgtc ttccctgttt gacgacatcc tcatctctga agcctgctct tcggaaaagc
     8461 ccgacggcaa acgcttccgc catcttcaag acaaatatgc cgacaaggca ggctgcttca
     8521 tctatatcgg cgacaacatt tccaaagact ttatcgcgcc gaacactttg ggctggataa
     8581 ccataggatt gcttccctcc ggacagaaca tccaccgcca ccagcctgaa agttttaaca
     8641 cagaatatca tcccgacttt tgggttaact cccttcaaga tttagttcct ataattgatt
     8701 caaatactta accattacat aatcaaacat gcactcacaa tattaaaagg actatttcat
     8761 gacaaaatat tattcatggg gcgtaaacgg ctttccgccc tacgttttcg acaccatttg
     8821 gaaacgatgg aagttgaatg ggcgcggggg cggttatcgt gtgcgacatc ccggacggca
     8881 tgaccgtcgc gggcaatccg gcaaagccgc taccgcgcaa aaaccccaag accgggacgg
     8941 cataagcg
//
LOCUS       DAWGJV010000081         8716 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWGJV010000081 DAWGJV010000000
VERSION     DAWGJV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.081"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            191..1726
                     /locus_tag="WHQ94_001346"
     CDS             191..1726
                     /locus_tag="WHQ94_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2128612.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WHQ94_001347"
     CDS             1723..1815
                     /locus_tag="WHQ94_001347"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ2128613.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WHQ94_001348"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WHQ94_001348"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ2128614.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="WHQ94_001349"
     CDS             complement(3106..3276)
                     /locus_tag="WHQ94_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ2128615.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WHQ94_001350"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WHQ94_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ2128616.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="WHQ94_001351"
     CDS             3697..4713
                     /locus_tag="WHQ94_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ2128617.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="WHQ94_001352"
     CDS             4739..5158
                     /locus_tag="WHQ94_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ2128618.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="WHQ94_001353"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="WHQ94_001353"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ2128619.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5831..8644
                     /gene="polA"
                     /locus_tag="WHQ94_001354"
     CDS             5831..8644
                     /gene="polA"
                     /locus_tag="WHQ94_001354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ2128620.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMAQLTAPDGAPTGAMYGVLNML
                     RRLRADYVHDYCAVVFDAKGKNFRHEMFPDYKATRPPMPDDLRPQAEALPDLVRLIGW
                     PVLVIPQVEADDVIGTLAAMAGEAGWNVVVSTGDKDMAQLVNERVTLVNTMSGETLDI
                     EGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAAE
                     IKGKVGENLRAALQRLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLVVDFKR
                     WGFRTWLKEAESRMHEAADGDLFGSDSIGEQAALNAEVPSEKQPELAPTPEKLDYQAV
                     TTEAQLAALLDKLSQADTIGIDTETTSLDAMNASLVGISIAFQAGEAIYIPVGHSLTA
                     APEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYII
                     ESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALRL
                     EAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLEQ
                     EAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPLP
                     KIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPIR
                     TAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAAE
                     VFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPGV
                     AEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNTNARAGAERAAINAPMQGTASDLI
                     KRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKV
                     DGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2036 a         2265 c         2310 g         2105 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc taaggtttcg
     1921 cgtcggcgga tgagtttgta tccgccaccg ttaaccaaca cttccgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggctccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc
     2101 gggccgacga tgttggcagt cagcggcgcg atgttttttg gttctaccgc ttcgatgtga
     2161 tgataggcat catatagggc ggggcgcatt aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacacgcg tcaacaacgc gcctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaaccgctt gggcatacgc gcccaaatca ggcacttttt cgttttggta aacaatgccg
     2401 acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggttag ttgggagccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gataccgaat ttgttggctt tcaaacctgt ggagatgtaa
     2641 ggatgggttt tcgcatcgac atcgggattg acgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgcgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatatttt cgttttcatt tttgggttgg
     3121 aggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgccaaaag
     3541 cggcggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg acggacggga
     3601 tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgctgtat ggtttccgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgctgt cgttaccgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagttt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttggaa tcattttcca aacatatccc ttatggtgtt gtctttgaag acgacggcga
     4861 cacgggctac ttctatgccg cttcgcaaga cgggattcta gatgccttgc acatctacaa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacatccat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcgtc ttggatgatg aattgctgga caaaatccta tcccgaaaat ctacataacc
     5161 cgcacaaaag gataacaaaa tgcccctgtt agacagtttc aaagtcgatc acacccgtat
     5221 gcatgccccc gccgtacgcg tggcgaaaac catgaccacg cccaaaggcg acaccattac
     5281 cgtgttcgac ctgcgctttt gcgttcccaa caaagaaatc ctgcccgaaa aaggcataca
     5341 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtcga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttc tacatgagcc tcatcggtac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg tggccaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgcttgcc gaagcgcaac aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtgcgg
     5761 ggtcgggttt taagccggca tcgtccttcc ctattttttc tgtcccttat cggttttaag
     5821 cgggtttttt atgtccaaca gacctacact cctcctcgtt gacggctcgt cctacctcta
     5881 ccgcgcgtat cacgcgatgg cgcagttgac cgcgcccgat ggtgcgccga cgggcgcgat
     5941 gtatggcgtg ttgaacatgc tgcgccggtt gcgggcggat tatgtgcatg attattgcgc
     6001 ggtggtgttt gatgcgaagg gcaagaattt ccgccacgag atgttccccg actacaaggc
     6061 gacgcgcccg ccaatgccgg acgatttgcg tcctcaagcg gaagccttgc cggatttggt
     6121 gcggttgata ggctggccgg tgctggtgat tccgcaagtc gaagcggatg acgtgattgg
     6181 cacgctggcg gcaatggcag gcgaagcagg ttggaatgtg gtggtgtcca ccggcgataa
     6241 ggacatggcg cagttggtga acgaacgcgt aacgctggtg aacacgatga gcggcgaaac
     6301 gctggacatt gaaggtgtga aggcgaaatt cggcgtgcgc cccgaccaaa tccgcgatta
     6361 tctcgcgctg atgggcgaca aggtggacaa cgtgccgggc gtggaaaagt gcggcccgaa
     6421 aacggcagtg aaatggctgg aagcctacgg ctcgctggcg ggcgtgatgg aacacgcggc
     6481 ggaaatcaag ggcaaagtgg gcgagaatct gcgtgccgcc ctgcagcgcc tgccgctgtc
     6541 gtatgatttg gtgacgatta aaaccgatgt ggacttgcac accgaacttt cagacggcat
     6601 cgaaagcctg cgccgtaccg cgccgaaatg ggcgcaactg gttgtcgatt tcaaacgctg
     6661 gggcttccgc acctggctga aagaagcgga aagtcgtatg cacgaagcgg cggacggtga
     6721 tttgttcggc agcgacagca tcggcgagca ggcggctttg aatgcggagg tgccgtctga
     6781 aaaacaaccc gaacttgccc cgacccccga aaaactggat tatcaagccg ttaccaccga
     6841 agcgcagctt gccgccttgt tggacaaact gtcgcaggcg gacacaatcg gcatcgatac
     6901 ggaaaccacg tcattagacg cgatgaacgc ctcgttagtc ggcatcagca tcgcgttcca
     6961 agcaggcgaa gcgatttaca tccccgtagg acacagcctg accgccgcac ccgaacagct
     7021 tgatttgcaa gacgtgttag gccgtctgaa accgcatttg gaaaaccccg ccctgaaaaa
     7081 aatcgggcaa aacctcaaat atgaccaaca tgttttcgcc aattacggta tcgccctgaa
     7141 cggcatcgcc ggcgacgcca tgctcgcttc ctacatcatt gagagccatc tcggacacgg
     7201 cttggacgaa ttgtccgaac gctggctggg cttggaaacc attacctacg aatcgttgtg
     7261 cggtaaaggc gcgaagcaaa tcggttttgc cgatgtcgcc atcgggcagg cgaccgaata
     7321 cgccgcccaa gacgccgatt tcgccctgcg cctcgaagcg cacctgcgcg cacaaatgga
     7381 cgccaaacag cttgaaatgt atgagaaaat ggagctgccc gtcgcgcagg tattgtttga
     7441 aatggaacgc aacggcgtgc aaatcgaccg cgccgaactc gcccgccaaa gcgcagaact
     7501 cggcgccgag ctgatgaagc tcgaacaaga agcctatgcc gccgcaggac agccgttcaa
     7561 cctcaactcg cccaaacagc ttcaagaaat cctgttcgac aaaatgggca tcccgacaaa
     7621 agggctgaaa aaaaccgcca agggcggcat ttccaccaac gaggccgtgc tcgaacagct
     7681 cgcgcccgac taccccctgc ccaaaatcat cctgcaaaac cgcagcctgg caaaactcaa
     7741 atccacctac accgacaaac tgcccgaaat gatttccccc aaagacggcc gcgtgcatac
     7801 cacctacgcc caagccgtcg ccattacagg acgcttggca agcaacaacc ccaacctgca
     7861 aaacatcccc atccgcaccg ccgaaggccg acgcgtgcgc cgcgccttca ccgcaccgca
     7921 aggcagcgtc atcgtttccg ccgactattc ccaaatcgag ctgcgcatca tggcgcacct
     7981 ctccggcgac aaaaccctca ttgccgcgtt ccaaaacggc gaagacgtac accgccgcac
     8041 cgccgccgaa gtgttcggca tcgcccccga aaacgtctcg tccgaacaac gccgctacgc
     8101 caaaaccatc aacttcggcc tgatttacgg catgggccaa tacggccttg ccaaatcatt
     8161 gggcatcgac aacatctccg ctaaaaactt tatcgaccgc tacttcgccc gctaccccgg
     8221 cgtcgccgaa tacatgcagc gcaccaaaga acaagccgcc gcccaaggct ttgtcgaaac
     8281 cctgttcggc agaaggctct atttgcccga catccgcaac aaaaacacca acgcccgcgc
     8341 cggagccgaa cgcgcagcca tcaacgctcc catgcagggc accgcgtccg acctcatcaa
     8401 acgcgccatg atagacgtgt cccgctggct ttcagagtgc gaagcctccc cgtgggacga
     8461 actcttacaa agcaaactga ttatgcaggt gcatgacgaa ctggtgctgg aagtcgttga
     8521 aaccgaactg gattttgtca aagaaaaact gccgcagatt atggcgaaag tggacggcgg
     8581 attattggat gtaccgctgg tggctgaggt tggcgtaggg gagaattggg aagaggcaca
     8641 ttgattgaaa ggtgttatat gctatcttta tttaaataaa atttaatttt tggtatattt
     8701 tttctaaatg ttccta
//
LOCUS       DAWGJV010000082         8692 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWGJV010000082 DAWGJV010000000
VERSION     DAWGJV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.082"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            207..1130
                     /locus_tag="WHQ94_001355"
     CDS             207..1130
                     /locus_tag="WHQ94_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2128621.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1195..2583)
                     /gene="fumC"
                     /locus_tag="WHQ94_001356"
     CDS             complement(1195..2583)
                     /gene="fumC"
                     /locus_tag="WHQ94_001356"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ2128622.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2724..4703)
                     /gene="tkt"
                     /locus_tag="WHQ94_001357"
     CDS             complement(2724..4703)
                     /gene="tkt"
                     /locus_tag="WHQ94_001357"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ2128623.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQATVLPEGLPRVAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5232..6728
                     /gene="ccoG"
                     /locus_tag="WHQ94_001358"
     CDS             5232..6728
                     /gene="ccoG"
                     /locus_tag="WHQ94_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ2128624.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6731..7279
                     /locus_tag="WHQ94_001359"
     CDS             6731..7279
                     /locus_tag="WHQ94_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ2128625.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAIRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(7339..8514)
                     /locus_tag="WHQ94_001360"
     CDS             complement(7339..8514)
                     /locus_tag="WHQ94_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ2128626.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDYAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
BASE COUNT         1882 a         2122 c         2500 g         2188 t
ORIGIN      
        1 gcaacgctgt actggttttt gttaatccac tatattttaa aattcaggcg gtgttttcaa
       61 atagcgcgaa gcaatagccg agcttcagcc ggtttgtttg aaatcaaagg gttggaatta
      121 aacgccaaac gcttgaacca ttattgcccg atgcggcaaa ccggcatcgt ccggcaatac
      181 ggttgtttaa aaataggaaa atcaggatgg aaaaccaaag gccgctccta ggcttcgcgt
      241 tggcactttt ggcggcgatg acgtggggaa cgctgccgat tgccgtgcgg caggtattga
      301 agtttgtcga tgcgccgacg ctggtgtggg tgcgttttac cgtggcggcg gcggtattgt
      361 ttgttttgct ggcactgggc gggcggctgc cgaagcggcg ggatttttct tggtgctcat
      421 tcaggctgct gctgctcggc gtggcgggca tttcggcaaa ctttgtgctg attgcccaag
      481 ggctgcatta tatttcgccg accacgacgc aggttttgtg gcagatttcg ccgtttacga
      541 tgattgttgt cggtgtgttg gtgtttaaag accggatgac tgccgctcag aaaatcggct
      601 tggttttgct gcttgccggt ttgcttatgt tttttaacga taaattcggc gagttgtcgg
      661 gtttgggcgc gtatgcgaag ggcgtgttgc tgtgtgcggc aggcagtatg gcatgggtgt
      721 gttatgccgt ggcgcaaaag ctgctgtcgg cgcaattcgg gccgcaacag attctgctgt
      781 tgatttatgc ggcaagtgcc gccgtgttcc tgccgtttgc cgaaccggca cacatcggaa
      841 gtttggacgg tacgttggcg tgggtttgtt ttgcgtattg ctgcttgaat acgttaatcg
      901 gttacggctc gttcggcgag gcgttgaaac attgggaggc ttccaaagtc agcgcggtaa
      961 caaccttgct ccccgtgttt accgtaatat tttctttgct cgggcattat gtgatgcctg
     1021 atacttttgc cgcgccggat atgaacggtt tgggttatgt tggcgcactg gtcgtggtcg
     1081 ggggtgcggt tacggcggcg gtgggggaca ggctgttcaa acgccgctag ttcgcaggca
     1141 acggaaaatg ccgtctgaac gaggtttcag acggcatttt atttgaggga aagattagcg
     1201 cggatgaacc atatcggcag gaacgaccag ttcgtcaaat tcttcgcccg tcagcaagcc
     1261 cagctcaacg gcggtttcgc gcaacgattt gttgtttttg taggcggttt tggcgacttt
     1321 ggcggcgttt tcgtaaccga ttttgcggtt caacgcggta acgagcatca gggaatggtg
     1381 caggaaatag tcgatttttt ccggtacggg ttcgatgccg acggcgcagt gttcgttgaa
     1441 gctgttgcac gcgtcgccca agaggcggat ggattgcaag aggttgtagg cgataacggg
     1501 catatagacg ttcagctcga aattgcccga cgcgcccgcc ataccgatgg taacgtcgtt
     1561 gccgaacact tggcagcaca ccatcgtcat tgcttcgcat tgggtcgggt tgactttgcc
     1621 cggcatgatg gacgaacccg gctcgttttc ggggattttg atttcgccca aaccgcaacg
     1681 cgggccgctt gccagccagc ggatgtcgtt ggcgatttta ttcaggcttg ccgccagcgt
     1741 tttcaatgcg cccgaagcgg caacggcggc atcgcgtccg cctaaggctt caaatttgtt
     1801 cggcgcgctg acaaacggca agccagacag ttcggcgagt ttagcagcag ctttttcggc
     1861 gtattcggga tggctgttca aacccgtgcc gaccgccgta ccgcccaaag caagttcata
     1921 caagtcttta agcgcatcgt tcagacggcc taaaccgtga tcaagctggg aaacgtagcc
     1981 ggaaaattcc tgtcccaaag tcagcggcgt cgcgtcttgc aagtgggtgc ggccgatttt
     2041 gacgataggg gcgaaagctt gggctttttt gtccaacgtg tcgcgcaggg cttttacggc
     2101 ggggatgagg tggcggttga tttcaatcgc ggcggcaacg tggatggcgg tcgggaatgc
     2161 gtcgttggtc gattgcgcgt ggttcacatg gtcgttggga tggacgggct gatacgccgc
     2221 caaacctgta cctgcaattt cgttggcgcg gttcgccagc acttcgttca tgttcatatt
     2281 ggactgcgtg ccggagccgg tctgccaaac cactaaagga aactgtccgt caagcctgcc
     2341 gttcaacaca tcatccgccg cctgcgtaat caaatccgcc tgttcgggct taatcctgcc
     2401 gagggaaaca ttggtggcgg cagcggcttt tttcaccagc gccaaagcat aaatcagcgg
     2461 ctgcggcagg gtttcgccgc cgattttgaa attgttgcgg ctgcgctggg tctgcgcgcc
     2521 ccaataggct tcggacggga cttcgacatt gcccatggtg tcgtgttcgg tgcgagtgtt
     2581 catacgtttt tccttttgaa atgtgaataa gagtgattcg caaatattat aatggagatt
     2641 ggtggaatga ggaagcatca aggcgggagg gcaaaaaaat gccgtctgaa aagcgttcag
     2701 acggcatttg tttaggagtg gggttacagc actgatttca ctgtatcgac cacattgtcc
     2761 acggtaaagc cgaatgcttt gaagagtaag tcggcagggg cggattcgcc gaagcggttg
     2821 atgccgacga ctgcgccgtt cagaccgacg tatttgtacc agccgtcggc gtgtccggct
     2881 tctacggcaa cgcgcggcag accttcgggc aggacggtgg cttgataggc ggcgtcttgg
     2941 cggtcgaata cgttggtgga cggcatggaa acgacgcgta cggcgatgtt ttgcgcggcg
     3001 agggcttttt gcgcttccaa agccagctcg acttcggaac cggtggcaat gatgacggct
     3061 tgcgcgttgc cttgggcttc gctgatgatg tagccgccgc gtttgatgtc gttcagttgt
     3121 tgttcgctgc gcgcttggaa tttcaggttt tgacggctga aaatcaggca ggacggatga
     3181 tcggcggctt tgacggcttc tgcccaagcc accagagatt cggcggtgtc gcacggacgc
     3241 catacgtcca tgttcggaat caggcgcagg gtggcggttt gctcaatcgg ctggtgggtc
     3301 gggccgtctt cgcccaaacc gatggaatcg tgggtgaaca caaatacagg gttgattttc
     3361 atcagtgccg ccatgcgcaa ggcgttgcgt tcgtattcgc taaacatcag gaaggtcgcg
     3421 ccaaagggtt ttacgccgcc gtgcagggca agaccgttca taatcgcacc cataccgaac
     3481 tcgcgcacgc cgtagtggat gtagttgccg cctttgtcgc gggtaacgga gacgctgttt
     3541 gaccagtcgg tcaggttgga cggggtcagg tcggccgaac cgcccaccaa ttcaggcaac
     3601 tcttttgcca agatttcgat gctgttttgg ctggctttgc gggtagcgat ggtttcggct
     3661 ttggcgcaca cttctttcaa tgccgtctga acgtattcat cgaaattgtc cggcagcttt
     3721 ttatccatac ggcgcacaaa ttctgcggct tcggcaggat atttggcttg atattgcgcg
     3781 aacagttcgt tccagtcggc ttccagtttc gcgccttgtt ctttggcatt ccacgcatcg
     3841 taaatttctt gcgggatttc aaaggcgggg taagtccagc ccaaatgttt gcgcgtggct
     3901 tcgatttcgt ccgcgcccaa aggtgcgccg tgggttttgt ggctgccttc tttgttggca
     3961 ctgcctttgc cgattaaggt tttgcagcag atgatggacg gtttgccggt ttcggcacgt
     4021 gcggcttcga tggcggcttg aatggcggcg gtgtcatgac cgtttacgtt aggaacgacg
     4081 tgccagccgt agctttcaaa gcgttgcggg atgttttcgg taaaccagcc gtccacttta
     4141 ccatcaatgg aaatattgtt atcgtcatac aaaacaatca gtttgcccaa gcctaaagtg
     4201 ccggcgagtg agcaggcttc gtgcgatacg ccttccatca ggcagccgtc gcccatgaag
     4261 acgtaggtgt aatgatcgac gatgttcaaa ccgtctttat taaattcggc ggcaaggatt
     4321 ttttctgcca atgccatacc caccgcgttg gcaatccctt gccccaacgg gccggtcgtg
     4381 gtttccacgc cgtcggtgta gccgtattcg gggtggccgg gggttttgct gtgcagttgg
     4441 cggaagtttt tcaagtcttc aatgcttagg ttgtagccgg tcaggtgcag caggctgtac
     4501 aacagcatag acgcgtggcc gttggagagg acgaagcggt cgcggttgta gaatttgggg
     4561 ttggcgggat tgtgattgag gaatttcgtc cacaatgttt ccgccatttc cgccataccc
     4621 ataggcgcgc cggggtggcc ggaattggct ttttgaacgg catcggccga gaggaagcgg
     4681 attgcgtttg ccagttgaga cattttgtat tttccttgct ggtgtttcag ataagtggat
     4741 aatcggaaag cgttgattat cgcccgattc gcttatgctt tcaagaaaag ggcggacgcg
     4801 ggggaaggcg gcggcaggag accggtgggg aaggattttc gatttgcggg cgaagcctgc
     4861 cattattcct tttgaaataa aaagttatag attgtgtgcc ggattgtcga tagcgtttgt
     4921 ttatgagctt gcgccgtcgg ttctgccgat atgggggtgt cgattttttt agtctttgtt
     4981 ttttaaccgt attcggattt tgttcgggcg gtacggcaaa atccgggcgt tttgatgcgg
     5041 atgggaaatg tatccgcccc atacatccgg acggcgcata aagttgtaca atagcggaaa
     5101 tatattttgg gaaaaacgga ttttctcaaa acttgaaaca caacgcctta aaaaacaaga
     5161 aaaatgacgt ctgaaaagca aacggcggcg gtaacgggca aaccatcaat caaaaatcta
     5221 aggaatgcag aatgaccacg gaaaaccaag ccggcagtcc ggcatccgga atcggcacgt
     5281 ccgaacaaac aaaagccgcg ccgaaagcaa aaaaaacatt cgatccgcga gccagcgtga
     5341 ttcaaatcca tcccgaaggc gaacgcatcc atcccaaaaa ggcggaagga cggttcgcca
     5401 aactgcgtat cgccgccgta ttggcgacgc agtttgtgtt ttacgtcatt ccgtggttca
     5461 actggagcgg caggcaggcc gtcgttttca atatccccga acggcatttc ttcattttcg
     5521 gattgtcgtt gggggtgggc gatttgattt accttgcctt gctgctgatg atttgcgcct
     5581 tcgggctgtt ttggtggacg acgattgcag ggcgactgtg gtgcggctat tcctgcccgc
     5641 aaacggttta caccgaaatt atgctgtgga ttgacaacct ggtcgaaggc gatagaaaca
     5701 aacggctgaa actggaaaaa tcgccgtgga atttcactaa aatccgcatc aaagccacca
     5761 aatacctgct gattttcctt gtctgcgcgt ggacgggcat cacgtttgca ggctggtttg
     5821 tccctatccg ccagttcgtt cccgatttat tcactggagc agcaggtggc ggcgcgatgt
     5881 ttgccgcagc gttttatggc tttatgacct tcttcttcgc ccacattatg cgtgaaaaag
     5941 tatgcctgca tatgtgtccg tatgcacgtt tccaaagcgc gatgttcgac aaggacacgc
     6001 tgattgtttc ttatgacgcg gaacgcggcg aaccgcgcgg cgcgcgcaag aaaacggtca
     6061 ataaggaaga ggcgggtttg ggcgactgca tcaactgtgc gatgtgcgtc caagtctgcc
     6121 ccgtcggcat cgacatccgc aacggtctgc aataccaatg tatcggctgc gccgcctgta
     6181 tcgacgcgtg cgatgagatt atggacaaaa tgggctatcc gcgcggatta atccgttata
     6241 cgaccgaaag cgcgctggaa cacgaatatt ctgaaaaaga cattaaaaaa cggctgctca
     6301 gaccgcgtgt ggcaggttac ggcgcggtgt tggcgttagt tatcattgcc ttcctggccg
     6361 gtttgtccac gcgcaaaatg gtcgaggtcg atattttgaa agaccgtggc gtactggtgc
     6421 gcgaaaacgc caaaggctgg ctggaaaacg catacagcct gcgtatcatc aacaaaagtg
     6481 aaaaagaaca gctcattacc gcaagtgtca aaggctttga cgaaatcgcc ctgaccgggc
     6541 tgcccgaagg cggtatcaag gttgccccgc gcgaaacggt aacccttccc gtccaagtgt
     6601 ccaccattcc ggaatacgcg gacaaaggca gccaccctat cgaatttacc ttccaatacc
     6661 gcgaaagcgg cgcgcccgac ggcaagccgg tcgtcttgga agaagatgca acctttatcg
     6721 gagaataacc gtgtctcaaa acactccaat caaaccttgg tacaaacacg tctggccgtg
     6781 gatcttgatg gcggggccga tttttgtcgt catcgccagc gtcgctatgt tttttgtcgc
     6841 gcagcagcac gcgacagatt tggttacgga cgattattat aaagacggca aacatatcga
     6901 catccagctt catcgggatg aagaagccat cagacggcat atcggggtgc aggttctcat
     6961 ttcccccgat atgaatgcgg caaaagtgtt tgtcggcggc gagtttgacg gcaaacagcc
     7021 tttgaacctg ctgctgatgc acccgacccg caaggcggac gatcaaaccg tcgccctcaa
     7081 gcccgtcggc agcgcgcaga acggcagggc ggaatatgag gcggtgttca aaacccttcc
     7141 gccgaccaac cactggtatg tgcgcgtgga ggacgcggca ggcgtgtggc gcgtcgagaa
     7201 caaatggatt accagccaag gcaatgcggt cgatttgacc ccgatggaca aacttttcaa
     7261 taatactgaa agcaaataaa gagatgccgt ctgaaaattt tcagacggca ttttgataca
     7321 gtatcggctg ttttgacatc agcgtcctac tgttgcaaca ggttcgctcc ccgtcagcag
     7381 atattgtatc gtctcgcgga cggtgcggat ggcattaccg aagggcagcg ggtctttacc
     7441 tctgaagaac aggcctttat ctacttctcc acgaaatgcg gcagcaagct ggatatcaat
     7501 acagaattgc cctgctttgg aaagcccgtc gcgcaggccg cagctagtca ggcagtttaa
     7561 accttgggta cagcggcgcg ggtcggcttt ggcgtttgtc tgaagtttgc tttcacgctt
     7621 gatgtagctg tctaggaatt tggtgcggac accgcgcgcc ggcaaaccgg caacagacat
     7681 aaattcgact actttttcag tttccgcacc ggcgagcgtt tttttgaagt taaggtgtgc
     7741 atctccttct tcggtaacgg caaaagccgt accgatttga acggcggatg ctccccagtt
     7801 ctttagggcg gttttgactt tttcaaaatt tgccatgcct cccgcaagaa taagcgggat
     7861 tttttcgctt tccagcccta aacttttgaa aacttcaaac gtttcctcaa tcacgcgttt
     7921 gaagtcgaac ttggcatcgt ttacgccttc aacggttgat gcacccaaat gtccggccgc
     7981 gtgggcagga tgttcgacta caatcgcatc gggcaatatg ccttttttca tccaacgttt
     8041 caaaacgata ttaataccgc gcgattcgga cagaatcggc agcagcgcga catatttatg
     8101 atagccctcg gtcatttccg gcaggtctaa aggcagaccg gcacccatta caaccgcatc
     8161 cgcccctgat tcgcaagcct ggcggacata tgcggcgtag tctttgaccg ccttcatcac
     8221 gttgaccgca atcagccctt ttccctctga agcacttttg gctttttgga tttccctgtc
     8281 caatgcggta cagttcaaag acgtatattt ctcttcactc ggattgattt gggattcggc
     8341 gagtaggtct tcgtgaaggt ggcgcaaatc cacactggca atcgttccga taccgttttc
     8401 acgtgccacc gcgctggata aacccgatgc ggaaacaccg acccccatac cgccttgcac
     8461 gatgggggta agggattttc cacgaataac caaagggtca aaaatattct gcatcagttt
     8521 ctccgagtgt acgaatcagt caggctcatg aaaaatggtt ctaactgttt tagaactatt
     8581 gctcaaattt ggtattatac tctaaatatc gcggcggtaa acaagaaagc agccggagct
     8641 tcgggcatat tctgttacgc ctgtttcaat cgggagagaa aatattatag tg
//
LOCUS       DAWGJV010000083         8654 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWGJV010000083 DAWGJV010000000
VERSION     DAWGJV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.083"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WHQ94_001361"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WHQ94_001361"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ2128627.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVTLRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQAEEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGSK
                     EQADQVFAAALESGYNLRRVSDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPETQTAGYRELLTDMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3480)
                     /locus_tag="WHQ94_001362"
                     /pseudo
     CDS             complement(3122..3480)
                     /locus_tag="WHQ94_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3428..3658
                     /locus_tag="WHQ94_001363"
     CDS             3428..3658
                     /locus_tag="WHQ94_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ2128628.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3651..4292)
                     /locus_tag="WHQ94_001364"
     CDS             complement(3651..4292)
                     /locus_tag="WHQ94_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ2128629.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4282..4833)
                     /locus_tag="WHQ94_001365"
     CDS             complement(4282..4833)
                     /locus_tag="WHQ94_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ2128630.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4837..5355)
                     /locus_tag="WHQ94_001366"
     CDS             complement(4837..5355)
                     /locus_tag="WHQ94_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ2128631.1"
                     /translation="MRLFPIAAALTLAACGTAQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5355..7016)
                     /gene="pqiB"
                     /locus_tag="WHQ94_001367"
     CDS             complement(5355..7016)
                     /gene="pqiB"
                     /locus_tag="WHQ94_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ2128632.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTAFNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7009..8382)
                     /locus_tag="WHQ94_001368"
     CDS             complement(7009..8382)
                     /locus_tag="WHQ94_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ2128633.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCCDEAL
                     PPHTVDCPECGCRADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
BASE COUNT         1947 a         2202 c         2442 g         2063 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaacc agtctgccgt
      181 cgacgaaaat gccgtctgaa agtgtttcag acggcatttg tcttcggggt ttcaacggtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacacg gttgacgctc ggccagaatt tgtgttcgcg gacgaacggc aatgggaaga
      361 cggcttcttc gcgggaatac ggatgcgtcc attcgccagt tacgtcggat gcggtatgcg
      421 gagcgttcac caacgggttg tcctctttcg gccattcgcc gcgctcgact ttcaacactt
      481 cctgtttgat ttgtttcagg gcggcgatga agcggtcgag ttcggctttg ctctcgcttt
      541 cggtcggctc gatcatcagc gtgccggcaa cggggaagga gacggtcggg gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcggtaat gccgctttcg gctttgagcg
      661 gacgcaagtc gacgatacat tcgtgcgcga cgcggccgtt tttgcctgta tacagaatcg
      721 gatagtcttc gctcaagcgt ttggcaacgt agttggcgtt gagcaatgcc cagcgtgttg
      781 cctgctccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcaggatgg
      841 acgcagaacc gaaagctgcg gcagccacgg cagtttgatc ggcactggcg ttgtgcatat
      901 cggttaccac atggcctggt gcgaacggag ccagatgggc tttcaagcca atcggaccca
      961 tgcccggacc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga tgccgatttg ggcgttgagg ttggcgccgt
     1081 ccatgtaaac ctgtccgccg ttttcatgga tgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtagac ggataggtaa tcatgatggc ggacaaagtg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcgatgt tgacgttgcc gtgttcgtcg gtatcgacaa
     1261 cgacgacttt caagcccagc atggccgcag tcgcagggtt ggtaccgtgg gcggatttgg
     1321 gaatcaaaca gatgttgcgg tgtccctcgc cgttggcttc ttgataacgg cggatggaga
     1381 gcataccggt gtattcacct tgtgcgccgg agttgggttg caaggaaatc gcgtcaaagc
     1441 cggtgatggc tttcaggctg ttttccatgt ctgttaagag ttcgcggtag cctgcggttt
     1501 gggtttcggg ggcgtagggg tggatgtcgg agaactccgc ccaagtaatc ggcaacattt
     1561 ccgcagtggc attgagtttc atggtgcaac tgcccagcga aatcatactg cggttcatcg
     1621 ccaagtcgcg gtcttcgagt tttttcagat agcgcaacat ttcgtgttcg gtgtggtaac
     1681 ggttgaacac gggatgttgc agaatgtcgt cttgacgcag taattcggca ttcagacggc
     1741 ctttgacatc atcggcaaat gcgaacgtat ccttgccggt aaacgcgcgg tacaaatcga
     1801 ccaaatcttc gcgtgtcgaa gtttcatgga aggctgccgc aacttgagta tcgctgacgc
     1861 ggcgcaggtt gtagcccgat tccaaagcag cggcaaacac ttggtctgct tgctctttgc
     1921 tgccgaaatc gacggtaacg gtatcgaaga agaccttatg aaccacattc aggccgtctg
     1981 aaaccagcgc atcggcaaag gcagaagcca gcgcgtgaat gcggttggcg atgcgtttca
     2041 caccttcagg gccgtggtaa acggcgtaca tacccgccaa gttcgccagc aatgcctgcg
     2101 cggtacaaat attggatgtc gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatgcgcaa ggcaggtttg cctgatgcgt ctttggatac gccgatgatg cggcccgggg
     2221 cggaacgttt gaactcgtct ttaaacgcga aataagcggc gtgcggacca ccaaagccca
     2281 taggcacgcc gaagcgttgc gtgttgccca aagcaatatc cgcacctagt tcagccggag
     2341 acttcagcaa aaccaagctc ataatgtcgg ctgcaacggc aacgattgtg cctttggctt
     2401 tcagacggcc gataacgtcc tgcaagtctt gcacatcgcc gtctttgccg acgtattgga
     2461 acagcgcacc gaagtattcg ccttcctccg cttgggcaaa atcaccgacc accaattcaa
     2521 agccgaaata tttggcgcgg gttttcatca cgtccaaagt ttgcggatac acgcgcgcat
     2581 ccacaaagaa acgttcggat ttcactttgc ccactcggtg cgccatcgcc atcgcttcag
     2641 ccgcggcagt tgcctcgtcc agcaaagacg cgcccgccac gggaaaaccg gtcaaatcga
     2701 tgcacacttg ttggaagttc aacaatgctt ccaaacgacc ctgcgcgatt tccgcctgat
     2761 acggcgtgta ggcggtatac cagcccggat tttccaatac gttgcgcaaa gtcacattcg
     2821 gcacgcgggt cgggtaatag cctaaaccga tataggactt gttgatcacg tttttcgacg
     2881 caatgccttt caattttgcc aacgcgtccg cttcggtcag ggcatcgggc aaatcgagtt
     2941 cggacggcat acggatgctt tgcggcacgg tgttgccgac aaaatcgtcc atacttttct
     3001 cgccgacagc ggcaagcaac gccgcttcgt cgccgaaact caaatgccgc gcggcaaatt
     3061 cgtcggggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca atgcgccaga atgcagcagg tcaggacaag cagcgccaca agcggcagcg
     3241 ataagggaaa gccgccgacc aaaaccatga aacctgcagg caccagccaa gctaagggat
     3301 gcgccggaag cggaaaacaa tattgagcag aaccaaaatc acgacaaaca gcggcagcct
     3361 cgccatcagg ttggcacgcc tgtatgttgc gtacgggtcg gatgcttctt caggttcgca
     3421 atattccatg atttcagcct gtccgggctt tatccacagc aggctcggac ggtcggtcat
     3481 gccgtctgaa ccggcaggcc gtctttttca aaaaacgcca taccgctgca acttcatgcg
     3541 gctgtatggc gtaggttttg cacttcagac ggcatatgcg tcccgaagca gtttggtgtt
     3601 ccagtcggtt catgattata taaacaactt gggtgcggat aaacctttgt ttatctaaac
     3661 ccctgttttc tcaaaaattg attcaaatct tcggcaaatt tccgatagcc tttgccgttg
     3721 gcgtggattt ggtcggattt cagattatta tcgcccaaaa tttccgccca cgcgccgccg
     3781 aacagcggaa tgccgtattc ctcggacaaa tcctcataca gcggatggtc gctcaaatgt
     3841 ccgaacaacg cgcccagtgt gatgtgcggc acgccgacga ggacggcggg gatgttttcc
     3901 ttctgcacgg tttcgatgat tttcgcgata ttggcgcggg tctgctcctc gggaactttg
     3961 cgcagaaagt cgttgccgcc tatgccgaca agcacaagct tgggtttgcg tgccaacagc
     4021 gcgggcaggc gcgacagggc ttgggcggat gtatcgcccg atacgccgcc gttgacaata
     4081 ttccaacccg tcagtttttg cagttgcgcg gggtaggatt cgccagggtt tgcgccgtag
     4141 ccgaaggtaa gcgaatcgcc caaggcaagt acggtgcttc cttcgggaat tttggcgtgg
     4201 gttcgggcgg attttctgcc gcaggcggta aggagcaacg cgcctgcgcc gaggaggaag
     4261 gttcttctgt tcattggttt ttcagatggc atcgagccat ttcgggtggt atttcagggt
     4321 ttcacggtaa accggacagc tttcggcgtg cgcgcctttg aggtagccgg tactcatcag
     4381 aaattcgccg acgatttcgc cgccgacgaa tttgaaatgt tttttaaaga gtttaaccca
     4441 ttcgtctttg cttcgcggat ggtgcgtgtc gagccagttc ttgaacgagc cgtattcttg
     4501 ttgcaacgct tggatttgcc gtgcattgaa aatggcggca tcgattttca ggcggttgcg
     4561 gacgatgccc gcgtcggcaa gcaggcgttc gcggtcggtg tcgtcgaagg cggcaaccgt
     4621 atcgatgtcg aaaccttcaa atgccgtctg aaacgcctgc cgcttcttca gcatcagtgt
     4681 ccagtttaat cctgcctgat tgatttccaa caccagccgc tcaaacaatt cattgtcgtc
     4741 ctcaatcgga aaaccgtatt gcgtgtcgtg gtaatgtttg ttcgggttat cggtgttttc
     4801 gggaagtgag gcggcaaatt cgcaatagtt cacgttttac tcgaccatct gttgcgccgc
     4861 ctgtttcagt ccctgttcga gtgcggcggt catcgcggcg tagccgtcac cctgctgttc
     4921 ggtttcgata tggaagggtc tgttcgtacc gtcgggtagg acggcgtagc cgctgatgag
     4981 ggttttgccc gtgtagctgc cttggaatgc gtcaatatag accgtccatt tttcggtact
     5041 gccgctgcgt gaggcaggaa caaagatgcg tgtgctgtcc aaacggttga atgcattgct
     5101 caacgccgct tcgagcatat cgtccaaggt gtccgcccaa acgtggtttt gtgcggtgtt
     5161 gaggcggtag gggtcggttt gatagaccag tccgccgcgt ttgagcggtt cggcaagacg
     5221 gacttcgacg gcagtttcgc cgccttgcgt tgcaggacgg atgtagcggc tgtcgggcaa
     5281 cacgaaatat tgtgtgcttt gcgcagtacc gcaggcggca agcgtcaggg cggcggcaat
     5341 cgggaaaagg cgcattatcg gcttcctttc gggatggggt ctttgctgct gctgttgaaa
     5401 atcagcgcgt tgggtttttc tttcaaagta ttaattacgg gttgaacgtc ttttaaggtt
     5461 ttgtccaaac tttgcaacgt attttgtacg tcgccgtaga taggcgattg aggcgatacg
     5521 ccttgcaggg ttatgcgcaa ctctttcagg gtttggttca gttcgttcgg aatgttttgt
     5581 gtctgcggat tgccggccag tttgtcaatg gagcttaggg cggcattggc agatttgagt
     5641 gtggatttga gctcggcaag cgaaccgttc aattcggcaa ccgtcttatc taaaggcagt
     5701 ttgtcgaact tgtccagcaa atccaccact ttcgcctgca aatcgtccaa accgccgcct
     5761 tgtgtagcga tgacggtatc gcctgcataa acggtatgcg gtcgcagctt aggtgatgcg
     5821 gaaggctgat cgttcaactc aatcattttg ctgccggtca gcaggttgtt gctggagatg
     5881 gtggcggtca ggcctttgtt gaaagccgtc tgaaattgtt gtttccaatg ctctttgctt
     5941 tgttcgtcgg cattgatttc caaacgggaa ggctcaatgc ggatgcgcac gggaatccag
     6001 ccgttttcaa acaggtgcag gctgtcgttg cggtcaaaat aagggacatc ggaaaccacg
     6061 ccgacattca gccctttgta ttcaacaggc gaaccgacgg tcagtccgcg cacggattgt
     6121 ttgaaaaacg cggtgtagta cagcgagcgg tcgtcaggca ggttggcgac ttcgctgcgg
     6181 ctgtcgtaaa gcgtgaagct gtcttcgctt ttgacgtttt tactgttttt ggttttcggc
     6241 gaatcaaatg aaatcgcgcc cgacagcagg gcaggcagag gggcggaatt gagtttgatg
     6301 ccgctgcctg tggtttcgat attaatgccg ctttccagcc aaaaacggct ggcggaatga
     6361 atcagtttgt cgttggggct ttggatgaag atggtgtaat gcacgctttg gtcggacggg
     6421 tcgaaatgcg cgctttcgat ttgcccgacc ataaagtttt catacaaaac agggctgttg
     6481 acgttgagga tacggtcgtt tttaccaatc aaattcaagc gcagaccgct ttgcccgatg
     6541 gcggtaacgg gcggaatgtc ctgcacttgg aacacgtctt ttgcctcgtc gcttttgccg
     6601 ggtgtaaagg cgatgtacga acccgaaagc agcgtaccca aaccggttac gccgctttgg
     6661 tcgatacgcg gcttgaccac ccaaaactgt gtgtcgctgc ggatgaggcc ggatacgtcc
     6721 gcattgagtt gggcggttac ttccacgcct ttttggtcgt cgcgcagttt gattcgggta
     6781 acgcgtccga catcgatgct caatacctta atgaccgtat tgttgacctc aatgccttcc
     6841 gcgctgtcca tcaagagcgt aaccacaggc cccctgttgc ggatttcctt aatccacagc
     6901 cagccaccgg caatcagcgc aatcagcggg accagccaga cggcggagag caaggtgttg
     6961 tttttgcgga cgcgtgcttg ggcgtgtccg tttggaggag ggctgttgtc agtcatgttt
     7021 ttccgtttcg ttgaaagcaa tgccgtctga agcgcgtttg tcccaaagca ggcgcgggtc
     7081 gaaataatag gcggacagca tcgtcagaat cacgaccagg cagaaataga ctgccgcact
     7141 gcccggaatg acgcgcgcgg cataagtgtg gaacgaacac atcaaaataa taatcacaaa
     7201 aatatcaatc atcgaccagc ggccgaccgc ttcggtgatg cggtagaggt gcgacaattt
     7261 ctttgcaccc gttggcaaag cgaagcgggc ggacgcaatc aaaaccgaca ttgccgcaat
     7321 cttcagtacc ggcaccaaaa tactcgcgct gaaaataacc gccgcaatca gcctgtcgcc
     7381 ctcgtcccac atataagcga tgccgttaag gatggtattg acctccgtgg cggcaggatt
     7441 ggacgaaatc ataatcggca ggatattggc agggaaatac aaaataaccg ccgccgtcag
     7501 aaacgccgac gaaatactca gacttttcgg ccgtcggcgg tacagttccg caccgcacac
     7561 gccgcagggg gattcggcac tgtcgcggaa atacaggcag cggctgcaac aggttttacc
     7621 ttccgatgcc gtctgaaccg cattatcccc cgtcagccgc ccgatttgaa aatacaccca
     7681 atgctgggga accgataccg aagtccgaat cagcataacc gacagcgcga acatcagata
     7741 aaacgccggc ccgaagcgaa cctctgccac agacgagagc ttgatatacg ccaccaaagt
     7801 ggaaacaaaa aacacatcca ccatcatcgc ctgtctcaag cgcaccatca cacgcgttgc
     7861 caaacgcagc gcaggatacg cctgtttccg tatcagcgcg gcatagacat acaggcacag
     7921 cagcagaaac agaaccggcg cgccgaaagt cagcacaaac atcacttcgg ccaaaaaacc
     7981 ataatcctga aacaccatca ggcgcatcat ctcgggcagc gaaaggacgg atgccgcacc
     8041 cggtatcccg acctcgatat acgtcatacc gtaagcaaac gccattaaaa tcagcgaagc
     8101 cgccgcatag gcgggcgggg cggaaaaagg atgcctgccc accctgaaga gtttgtgtcc
     8161 gcaacgggga cagaacgccg cttctccact gtccaaccgg ggtacatccg cgcggcagcc
     8221 gcattccgga caatctaccg tatgcggcgg cagggcttcg tcgcagcagt tgccgcgaag
     8281 gctgtggggg atattttcgg caaacggctt catcgggaaa gtggcaaata taagtggcgg
     8341 cattataaag gaaaaaaggc gatacaccca atcaggatgc aaaaccggca ggttcggcag
     8401 tatgaacaaa cccttggtgt ccggcaaaaa tattttttga gcggttgtcc gccgactgcc
     8461 gaacccattt gataaaatcc cgattctgta aagaatagta aaatacctgc ttaaaaacat
     8521 cacgcaaatg atattttttc caaaaatatg tgataataaa tttcgtttat gattctttat
     8581 tatttttata gtggattaac aaaaatcagg acaaggcggc gaagccgcag acagtacaaa
     8641 tagtacggaa ccga
//
LOCUS       DAWGJV010000084         8653 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWGJV010000084 DAWGJV010000000
VERSION     DAWGJV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.084"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..142)
                     /locus_tag="WHQ94_001369"
     CDS             complement(<1..142)
                     /locus_tag="WHQ94_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEZ2128634.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGY"
     gene            381..1280
                     /gene="crgA"
                     /locus_tag="WHQ94_001370"
     CDS             381..1280
                     /gene="crgA"
                     /locus_tag="WHQ94_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ2128635.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1507..4722)
                     /gene="carB"
                     /locus_tag="WHQ94_001371"
     CDS             complement(1507..4722)
                     /gene="carB"
                     /locus_tag="WHQ94_001371"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ2128636.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4751..5392)
                     /locus_tag="WHQ94_001372"
     CDS             complement(4751..5392)
                     /locus_tag="WHQ94_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128637.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            5554..5952
                     /locus_tag="WHQ94_001373"
     CDS             5554..5952
                     /locus_tag="WHQ94_001373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128638.1"
                     /translation="MRYVCTRSPFSLQPQPTACVPCGTLSADEFKGRLKKRSQRLFHL
                     PCDKGCLKHSRCRCFGHSFSDDLQRLNLLQPAPSPVGEGWGEGIPRISATFPNTLTAK
                     TQALWLVALSLTLSHGREDWAVVGAKVSVN"
     gene            complement(6060..6449)
                     /locus_tag="WHQ94_001374"
     CDS             complement(6060..6449)
                     /locus_tag="WHQ94_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ2128639.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7022..7147)
                     /locus_tag="WHQ94_001375"
                     /pseudo
     CDS             complement(7022..7147)
                     /locus_tag="WHQ94_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7201..8334)
                     /gene="carA"
                     /locus_tag="WHQ94_001376"
     CDS             complement(7201..8334)
                     /gene="carA"
                     /locus_tag="WHQ94_001376"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ2128640.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1925 a         2200 c         2272 g         2256 t
ORIGIN      
        1 catagccggc atcaatcacg gtttcttgaa cattgtgtcc gagcgcggtc aaaacttctt
       61 tcgctttttt gtgaagcgtg cggtttaacc cgccgtgaga atgtccgaac gctttgccgc
      121 cgtctaataa taaaatattc atttgttacc tcgtttgtga attgatgaat gtattgtgcc
      181 gttttacttt ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata
      241 atcagatgtt ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt
      301 cggaaggggt tacacccctc ccaaataaag tctgatcctg ccgccctaaa gggcggggtt
      361 ccaaccgaaa aggaaacacg atgaaaacca attcagaaga actgaccgta tttgttcaag
      421 tggtggaaag cggcagcttc agccgtgcgg cggagcagtt ggcgatggca aattctgccg
      481 taagccgcat cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc aaccgcacca
      541 cgcggcaact cagtctgacg gaagaaggcg cacaatattt ccgccgcgcg cagagaatcc
      601 tgcaagaaat ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg
      661 tgttgcgcgt ggattccgcg atgccgatgg tgctgcatct gctggcgccg ctggcagcaa
      721 aattcaacga acgctatccg catatccgac tttcgctcgt ttcttccgaa ggctatatca
      781 atctgattga acgcaaagtc gatattgcct tacgggccgg agaattggac gattccgggc
      841 tgcgtgcacg ccatctgttt gacagccgct tccgcgtaat cgccagtcct gaatacctgg
      901 caaaacacgg cacgccgcaa tctgcagaag agcttgccgg tcaccaatgt ttaggcttca
      961 ccgaacccgg ttctctaaat acatgggcgg ttttagatgc gcagggaaat ccctataaga
     1021 tttcaccgca ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt
     1081 gcggtattgc ttgcttatca gattttttgg ttgacaacga catcgctgaa ggaaagttaa
     1141 ttcccctgct cgccgaacaa acctccaata aaacgcaccc ctttaatgct gtttattaca
     1201 gcgataaagc cgtcaacctc cgcttgcgcg tatttttgga ttttttagtg gaggaactgg
     1261 gaaacaatct ctgtggataa ttctataaaa acctgccata aaattgtttt tactcactta
     1321 ataactttga gaaaaaataa ataagatgat gtatagtcgg cggcaatggc agtctatatg
     1381 gggagcgtga tagggctgtt ccttatgtac ggcttgtgtg tggcaacatc ggctttccaa
     1441 atgacaaaat gccgtctgac accgtaaaaa aaggtttcag acggcatttt caacccgatt
     1501 caggcatcag ctgcggtttt tcaaacgccc gtgcagttct tgaacgctgt acacgcccaa
     1561 gtggtcgcgg cttttcgcgc cttcgctcat cgcttcgccg cccgcagtag tggtgtattg
     1621 cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg ctgtcggaca cggattgcgg
     1681 atcgctggaa acggtattca cgaccagtgc gatttcgccg tttttcagcg cgtcgccgat
     1741 gtgcgggcgg ccttcgggga ctttattgat ggtttgtaca atcagcccgt gttcggtcag
     1801 gtattgcgcc gtgccgcgcg tggcgcagat gccgtagcct aaggcttgga agtttttggc
     1861 ggttttaatg acgcgttctt tgtcttcttc gcgcacggag aggaagattt tgccggtcgg
     1921 gttgaggcgt tcgcccgcgc cgagttgggc tttgtagtag gcttcgccaa agcttgcgcc
     1981 cacgcccatg acttcgccgg tggagcgcat ttccggtccc aaaatcgtat ccacgcccgg
     2041 gaatttgatg aatgggaaca cggcttcttt aacggcatag aaatcgggga caacttcttt
     2101 ttccacgcct tgttctttca gggaaatgcc tgccatgcag cgcgcgccga ctttggcgag
     2161 cggcacgccg gtggctttgg agacgaaggg cacggtgcgg ctggcgcgcg ggttcacttc
     2221 caatacgaac accacgccgt cttgtacggc aaactgcacg ttcatcagtc cgaccacgcc
     2281 cagcgcgtac gccatcgctt tagtttggcg gcggatttcg tcttggattt cttcgcttaa
     2341 ggagtagggc ggcagcgagc agccggagtc gccggagtgg atgcccgcct gttcgacgtg
     2401 ctgcatgatg ccgccgataa ccacgtcttt gccgtctgaa acgcagtcca catccacttc
     2461 aatcgcgttg ttgaggaaga aatcgagcaa cacggggctg tcttcggaaa cctgcacggc
     2521 ttcgcgcatg tatttttgca aggcttcggc agagtggaca atctgcatcg cgcgtccgcc
     2581 caaaacataa gacgggcgca cgaccagcgg atagccgatt tcttcggctt tgacgagtgc
     2641 ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc agacctaagt cgttcaacac
     2701 tttttggaag cgttcgcggt cttcggcggc gtcgatgctg tcggcggacg tgccgatgat
     2761 gtttacgccg ttttcaacca gcgcgttggc gagtttgagc ggggtttgac cgccgtaatg
     2821 cacaatcacg ccccacgggt tttcggtgcg gacgatttcc aacacgtctt ccaatgtcag
     2881 cggctcgaaa tagaggcggt cgctggtgtc gaagtcggtg gacacggttt ctgggttgca
     2941 gttgaccata atcgtttcaa aaccggattc gcgcagggcg agcgcggcgt gaacgcagca
     3001 gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg ccgccgagaa tcatcacttt
     3061 tttacggtcg gaaggacggg attcgcattc ttcttcgtaa gtggagtaaa gataggcggt
     3121 ttcggtggcg aactcggcgg cgcaggtgtc aacgcgtttg taaaccggat gcagcttcag
     3181 cgcgtagcgg tgttcgcgca cttctttttc ttttacgccc aaaagctgcg cgatgcgttt
     3241 gtcggagaag cctttgcgtt tcagacggcg tagggcggcg aaatccaaat cactcaaaat
     3301 gccgtctgaa accgattttt cttctttcac caagtcttcg atttgcgcca agaaccaagg
     3361 gtcgatggcg cagatttcgt ggatttcttc cggcgtgaag cccgcgcgga acgcgtctgc
     3421 cacaaacagc atgcgctcgg ggccggggtt tgccagttcg cggcggattt ccgctttgtc
     3481 ggagcttctc ggattgaagc cgcacaagcc tgtttccaag ccgcgcaggg ctttttggaa
     3541 actttcctga atcgtgcgtc ccatcgccat cacttcgccc accgatttca tctgcgtggt
     3601 caggcggtcg tctgcggcag ggaatttttc aaacgcaaaa cgcgggattt tggttaccac
     3661 atagtcgatg gacggctcga atgacgcggg ggttttgccg ccggtgatgt cgttgcgcaa
     3721 ctcgtccagc gtaaagccga ccgccagttt cgccgccacc ttcgcaatcg ggaagcccgt
     3781 cgctttggaa gccagcgcgg atgaacggct cacgcgcggg ttcatctcaa tcacaatcat
     3841 ctcgccgttt tcagggttca ccgcaaactg cacgtttgag ccacccgtgt ccacgccgat
     3901 ttcgcgcaat actgccaacg aagcattacg catgatttgg tattctttgt ctgtcaatgt
     3961 ttgcgccggc gcaaccgtaa tcgagtcgcc cgtatgcacg cccatcgggt cgaagttttc
     4021 aatcgagcaa atgatgatgc agttatcgtt cttatcgcgc accacctcca tctcgtactc
     4081 tttccagccg aggacggact gctcaatcag cagctcgtgc gtgggcgacg catcgaaacc
     4141 gcgttcgcaa atcgccaaaa actcgtcttt attgtaggca atgccgccgc ccgaaccgcc
     4201 catggtgaaa gaaggacgaa tcagggtagg gaagccgacc tgttcttgcg ccgccaaagc
     4261 ttcgttcatc gtgtggcaga caaaagattt cgggcaagac aaaccgattt tttccatcgc
     4321 ctctttaaag cggccgcggt cttctgcttt gtcgatggcg tcttccgtcg cgccgattaa
     4381 ttcaacattg tatttcgcca gcacgccgtt gcgcgccaaa tccagcgcac agttcagcgc
     4441 ggtctgaccg cccatcgttg gcagaatcgc atcgggccgc tctttggcaa taattttttc
     4501 caccgtctgc cacataatcg gctcgatgta ggtaacatcc gccatttcgg ggtcggtcat
     4561 aatcgtggcg gggttggaat tcaccaaaat gactttatag ccttcttcac gcaaggcttt
     4621 gcaggcctgt gcgcccgaat agtcaaattc gcaggcctga ccgataacga tagggccggc
     4681 gccgatgata aggatggatt ttaggtcggt acgtttgggc atggtgggtc tttcttttga
     4741 agaatttgga ttattgacga ttggtaactt ctctaattat ttctgcatct gttttttcat
     4801 cgcttggatt atctgaaaag aaaaaactgt aattactatc ggctttaaat ttttcgtata
     4861 gctctgggcg caaaattttt gctttttcac tattttccac gctcctgcga ttaggcttgc
     4921 cagccttatc tttcccattt ctacggacat catagcaata aatagtgtta ggagaattag
     4981 gttctaaacg agtagctaca atctttttat aaattatgtc taaatctttt ttagtttgtg
     5041 ttggaatgaa atctgcaaaa agattactaa aactctgaaa aatttctctg attttcgcag
     5101 caccagcctg aacattaatt ttaaaaaaat taataaaatc agtagcactt ttaaaatgaa
     5161 cttcataaaa atcagcatac gcaaatattg aattttcact attcaaatta aaaaactcta
     5221 attttgcttg tgcataatca tctggtctag gttcatcagg tttataccta gtcaaaataa
     5281 cggcgtattg cctttgatta taattaaaag gaaatgcttc aattattcta tttttgtttc
     5341 tcatatcatt aagcaaatac tttaagttat tgaaattaat aggtattgac atatttaaac
     5401 tcctgcgaat atgttgcaat aaacgtagca atcgtaggtt gggttgaaaa cccaacacaa
     5461 accgcttaat tgtctaaaat gttgggtttg ctaacctaac ttgtatatcg aaacccccgt
     5521 atcgtagcaa gccgtagccc gtatgtaggg tatgtgcggt acgtatgcac gcgttctcca
     5581 ttttccctgc aaccccaacc caccgcgtgc gtgccttgcg gcacactctc tgccgatgag
     5641 ttcaaaggtc gtctgaaaaa acgttcgcaa cgtttgttcc acctgccttg cgataaaggc
     5701 tgcctgaaac attcccgctg tcgatgtttc gggcattctt tttcagacga cctgcaacgc
     5761 ctgaatctgc tgcaacctgc cccctccccc gtgggggagg gttggggaga gggcattccc
     5821 cgaatttcgg caacctttcc taacactcta accgccaaga cacaagcctt gtggcttgtt
     5881 gccctctctc taactctctc ccacgggaga gaggactggg cggttgttgg ggctaaggtt
     5941 tctgtaaact aatcaggtta tgcaggctgc tttttgtttt tcagacggcc tttataaatg
     6001 ctcaattcag tcggctttat cctattattt tcaataatcc aatcaaaatc agccatctgc
     6061 tatcgtgcag cttgcttttc caattcttgc aacacgagca ggatttctgt cagcacatcg
     6121 tttgtctgct gtaaaatctc gtgattccaa aaccgcagta cggtaaagcc caagctgttg
     6181 agatatgccg tccgcgcgtg gtcgtatgcg gcttgttctg tgtgctgccc tccgtctgct
     6241 tcgacaatca acttgggcgt tacgcacata aaatcaacaa tatagtttcc catcggctgc
     6301 tggcggcgga atttatagcc gttcagacga cctgcccgca ggtgctgcca caattttgct
     6361 tccgccccgc tcatttcttg gcgcatggct ttggcgcgtt ggcgcaagat ggggttgtcg
     6421 gtggtcaaga gtttctcgtg cgggttcatt ttgttgtcgt gctgtttgct gccctctctc
     6481 taactctctc ccacggggag agaggacggg gcggttgttg gggttaaagg ggctgtaagg
     6541 aaaacaagtc gttcaggcca ctttttactt ttcagacgac ctgcaatcaa cggttgtaaa
     6601 tccaagttgc ttttcaaacc gcctgtaacc gcaaaacttg ctgcaaccta ccccctcccc
     6661 cgtgggggag ggctggggag agggcattct ccgaatcgag gcaatctttc cctaattccc
     6721 taactgcctc aatacaagcc tttcggcttg ttgccctctc tctaactctc tcccacggga
     6781 gagaggacgg tgcggctgta gtgattaagg gttttgcaag gaaaacaagg aaaacaagtt
     6841 gtacaggctg ttttttattt ttcaagcgac ctgaaatcaa cgcttgtaaa tccaagtcac
     6901 ttttcaaact gcctgcaacc gcaaaatctg ctgcaaccta ccccctcccc cgtgggggag
     6961 ggctggggag agggcatttt ccaagttgcg gcaatctttc ccaaatccct tagtcttcta
     7021 aaatacaagc cttacggttg ttgccaaatg aaacttcttc catacccaaa acttgcgtgt
     7081 aaaacgcgat ggttcggtca atgtcggcaa cagttagtac tagatggtcg agtgcgctaa
     7141 tcttcatttc cagctttgtt tttcagacgg cagcagcata ctgttgccgt ctgaaagcca
     7201 ttatgcccgt tttgccgcct tcatattgcc aatgaatttg tcaaacaaat agccgacatc
     7261 ttgcggaccg gggctggctt cggggtggcc ttggaagcag aacacaggtt tgtcggtcag
     7321 ctcgatgcct tgcaaagtgt tgtcaaacaa ggatttgtgg gtaatgcgtg cgttagcggg
     7381 cagggtgtcg gcatcaacgg caaaaccgtg gttttggctg gtgatgacga ctttgccgct
     7441 gtccaaatct tgcacagggt ggttcgcacc gtggtggctg aagcgcattt tcagggtttt
     7501 cgcgccgata gcgaggctga tgagctggtg tcccaagcag atgccgaaaa tcggtttgcc
     7561 gctttccatc agtttttgca cggcttcgat ggcgtaggtg caaggctcgg ggtcgccggg
     7621 gccgttggac aggaacacgc cgtcgggatt gagtgccaac acgtcttgtg cgctcgtttg
     7681 ggcggggacg acggtcaggc ggcagccgcg cgaggcgagc atacgcagga tgttggtttt
     7741 cacgccgaaa tcgtaggcga cgacgtggta aggctgttcg tcaggggtaa cgaaaccctt
     7801 gcccaattcc cattcgcctt ccgtccattc gtaagtttcc gtgcaggaaa cttcttttgc
     7861 caagtctttg ccgaccatgc tgccgaacgc ggcgatgagt tcttgcgctt tttcgattgt
     7921 ggcatccgca ccggtcagaa tcgcgccgcc ttgcgcgcct ttttcgcgca acagcgtggt
     7981 caggcggcgg gtatcgatgt cggcgatggc gacggttttg ttgcgtacca gatagtcgtg
     8041 caggctttcg gaggcgcgga agttgctgtg caagagcggc aggtcgcgga taatcaggcc
     8101 ggcggcataa acgctgcggc tttcttcatc ttcggcgttg gtgccggtgt tgccgatgtg
     8161 tgggtaggtg agggtaacga tttgtttgca gtaggacggg tcggtcagga tttcctgata
     8221 gccggtcatc gaagtattga acacgacttc gccggaagtc gaaccttcgt aaccgattga
     8281 tgtgccgtga aatacgctgc cgtcagcgag gacgaggagg gcgggggtgc tcatgatggg
     8341 aatcctgttt ttaataaaat tcttgacaat gccgtctgaa gggacttcgg cagggggcat
     8401 ccggtcggaa aaatccggtc aaaaaaaaca cgccgcgcaa gatagacgcg taacgtgctt
     8461 ttcgggcaag tgcctatacc ctgaaagcag catattttaa gacgaaacat acccgatttc
     8521 aagcgcaaac gcggcaggcg ggctgatatg ttcgcgcgaa aacggaaatc cggggcataa
     8581 aaccgcccgc tccgcgccca ctatgcccgt tacgcatttc cccacgtccg cccggcgaaa
     8641 ctatgggaat acc
//
LOCUS       DAWGJV010000085         8584 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWGJV010000085 DAWGJV010000000
VERSION     DAWGJV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.085"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            167..1351
                     /gene="coaBC"
                     /locus_tag="WHQ94_001377"
     CDS             167..1351
                     /gene="coaBC"
                     /locus_tag="WHQ94_001377"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ2128641.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1418..3574)
                     /locus_tag="WHQ94_001378"
     CDS             complement(1418..3574)
                     /locus_tag="WHQ94_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ2128642.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKMVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3667..3873)
                     /locus_tag="WHQ94_001379"
     CDS             complement(3667..3873)
                     /locus_tag="WHQ94_001379"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ2128643.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3932..4549)
                     /gene="gmk"
                     /locus_tag="WHQ94_001380"
     CDS             complement(3932..4549)
                     /gene="gmk"
                     /locus_tag="WHQ94_001380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ2128644.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4677..5276
                     /locus_tag="WHQ94_001381"
     CDS             4677..5276
                     /locus_tag="WHQ94_001381"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ2128645.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5322..6110)
                     /locus_tag="WHQ94_001382"
     CDS             complement(5322..6110)
                     /locus_tag="WHQ94_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ2128646.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6435..7790
                     /gene="yegQ"
                     /locus_tag="WHQ94_001383"
     CDS             6435..7790
                     /gene="yegQ"
                     /locus_tag="WHQ94_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ2128647.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7924..8400
                     /locus_tag="WHQ94_001384"
     CDS             7924..8400
                     /locus_tag="WHQ94_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ2128648.1"
                     /translation="MLAVCQSGSAPKNAHTASAGTPTGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2172 a         2340 c         2028 g         2044 t
ORIGIN      
        1 ctccaataat cccttcctcc ccctcctctg actggtgcgc ctttgtgaat atactgtctg
       61 aaacttaggg cttcagacgg cattttgtat ccaaacggta actaatgtat ccgcactttg
      121 ttatagaatg gctgctgttt tttcttcgta attagaaatt gtcaaaatgg gtaaacatat
      181 tcttttaggt gtaacgggca gtattgcggc gtataagtct tgcgagttgg ttcgtctgct
      241 gaaaaaacag gggcattcgg ttacggtggt tatgagccgc tcggcaactg aatttgtttc
      301 tccgctgact tttcaggctt taagcggcaa tcctgtcctg accgacacgc acggcggcaa
      361 cggttcaaac ggtatggaac atatcaacct gacccggaat gcggatgttt ttctgattgc
      421 gccggcaagt atgaataccg tggcaaaaat ctgtaacggc gtggcagata acctactgac
      481 cagtctggca gccgcacgga aatgtccgct cgccatcgct cccgcgatga atgtggaaat
      541 gtggctcaac cctgccaacc aacggaatat cgcacaactg gtttcagacg gcattactgt
      601 ctatatgccg ggcttgggcg aacaggcttg cggagaaaat ggcatgggaa ggatgccgga
      661 acctgccgaa ttgctggatt tgcttccgga cttgtggaca ccgaaaattt taaggggcaa
      721 gaaaatcttg attaccgcag gtgcgacatt tgaagccatt gaccctgtca ggggcatcac
      781 aaatacctcc agcggaaaaa tgggggtagc tttggcgcgg gcgtgccgtg ccgccggtgc
      841 ggaagtcagc ctgattcacg gacagcttca aaccgcgctg cctttcggca tatccgatac
      901 ggttcaagcc gtcagcgctg aagatatgca tcgcgcagtg catcgtttaa tcgacaaaca
      961 agatgctttt atttctgttg ccgccgtctc agactataag gttaaaaaca ggagtacgca
     1021 aaaattcaaa aaagataaaa atgccaaacc attatccatc gaattggatg agaaccccga
     1081 tattttggct tctattgcct cattgccgaa cccgccgttc tgcatcggtt ttgccgctga
     1141 aacggagaat gtaatggcat atgcgcgaga aaaacgtatt aagaaaaaaa taccagtgat
     1201 cgttgccaat gatgtttcaa tcgcgatggg caaaccgacc aaccggatta ccattatcgg
     1261 ggacgacgag gaactgtctt ttcccgaaac aagtaaagat gaagcggcaa tgcagattgt
     1321 tgaaaggctt gccgtatatt tgaacaaata agcaattgaa cggataaacc ataaagcggg
     1381 ttgcctgtta atcaaaaggc aacccgtttt acctgcttca acttctgatg actttgcgga
     1441 tatatggaat actatgcaga ttttgaataa tctgattcaa ttgattcaga ttcttgactt
     1501 tcaataagaa tttgaattcg acaaaacctt ccgttcccga ctgggattta gacggtgttt
     1561 cgaccgactc aatgtccgca ccggaatcgg aaatcgcttg cgccattaat gccagcaagc
     1621 cgtggctgtc ttccgattgg acttgaagcc cgacacggta gttctgcccg ttcatatttt
     1681 cccagtctgc atccagctgc tgttcgggat cggactttaa caacgtcggg caggtatccc
     1741 tatggataat catgcctttt cccttaacca acagcaaacg gatggaatcg ccgggaaccg
     1801 gatggcagca ctctgcaaaa tgaatatgcc cgctttcctg accatcaact ttaatggaac
     1861 tgagcctgac ctcgctgccg aaatgctccc ctgccaactc ggcaatgtgc atggcgacat
     1921 aaacaggcag ggtatgcccc ataccgacgt tgtacagcac ttcctcaaat gaagtctgtt
     1981 tgtcgttgag atcggcaagg tatttttctt taatgccgtc tgaaagcagg acatctttgg
     2041 gcagcaaact ggacagggct ttttgtaaga ggctctctcc caaaacgacc gcatcgtgcc
     2101 ggttaaggtt tttaatatat tggcgtatgg cactgcgcgc cctgcctgac acggcgaaat
     2161 tcaaccatgc aggattgggt ttggcatgtt cggatgtaat aatttcgacg gaatcgccgg
     2221 ttttgagctt cgtacgcaac ggcatcatga tattgttgat acgtgcggca acggttttgt
     2281 gcccgatatc ggtatgcacc gcataagcaa aatcgacagg cgttgcccct ttgggcaaag
     2341 ttaggatttt tccttttggc gtaaggatgt agatttcgtt cggaaacaaa tcgactttga
     2401 cgtgttcgag aaactcaatg gcattggcac tgcttgcctg caaatctaag atatttttca
     2461 gccaccggtt tgtgtgaagc accgcctgat cgaccatctt agaatatgat ttatagctcc
     2521 aatgtccggc gattccacct tcggcaacag catccatttc cttggtacgt atctgaactt
     2581 caatcggcaa gccgtaaggg ccgaccaaag tcgtatgcag actttgatac ccgttgcttt
     2641 tcggaatggc gatatagtct ttgaaccgcc cgggcttggg ctgatagagg gtgtgcaatg
     2701 cgccgagtgc ggcataacag gctggaatgc tgttgacaat gacgcggaaa ccgtaaatat
     2761 ccataacctc ggcaaagcgc agctttttcg ccatcatttt ctgatggatg ccgtacaggt
     2821 ttttttccct gcctttgatt ttggcctcta tattcgcgcc taccagccgc tggccgaatg
     2881 cgcgcaagac tttgccgaca acgtcctgcc ggttcttccg gctcttgtcc atcgcttttt
     2941 ttaaagtctc gtagcggttg ggatgcaggt tttggaacga taaatcctga agctcttgat
     3001 atgcgttatt caaacctata cggttggcaa tctgtgcata gatttcaagg gtttcccttg
     3061 caatccggcg gcgtttgtcc gggcgcatcg aaccgagtgt ccgcatatta tgcagacggt
     3121 cggcaagttt aacgacaatc acgcgcacat ctttggtcat tgccaaaatc agtttgcgga
     3181 aactctccgc ctgatgttcc gcatgatctt caaatttgag tttttcgagt ttggacagac
     3241 cgtccaccat ctcggcaatc gtattgccga acaccgccgc catttcccct tttgtcacgc
     3301 ccgtatcttc caatacgtcg tgcatcacgc ctgcacacag accctgtatg tccatatgcc
     3361 aaagggcgag ctgcgtcgca acggcaattg gatgcgtaat gtatggttcg ccacttttcc
     3421 gggtttgtcc gtcatgagcg tgaaatgcat aggcaacggc cttctcgagc tcaacctgtt
     3481 cttcaggctt gagataggaa gcggtacgga aaagcaaggt gcgtgcttcg gcagttaaat
     3541 ggtcgtaagg tgcggtgggt tgagggacag gcatttcaga cggctttcgg aacatatttt
     3601 atttttcgtt tcaaaccgtc ggactgcacg gcggcagaat gtgccgacgt gcgtttccgg
     3661 cagaatttat ttattgcgcg tcaacagttc cgtaccgata tgcccggcgg cgatttccct
     3721 taaggcggta acggtcggtt tgttattgcg gacatcgtcc acaagcggcg tattgccatt
     3781 ttcaagctgg cgggcccgac gggccgctac caatgtcagg tcaaaatggt tggaaatttt
     3841 tccggtacag tcttcggtgg taatacgtgc catattattt gctttctttc aaaaatattt
     3901 aaaataggaa actgggtatt ttcgcggttt tttaggaatt ttccaacaaa tctgcaataa
     3961 atcccagttg ccgcgacctt ttcagacggc aggcattcac aatatggcgc aaatcctcct
     4021 ccgcccgcgc caagtcgtca ttgaccacga caaagtcgaa caatacggac tgttcgattt
     4081 catgccttgc cttcgcaagc ctcctttgga taacctcctg actgtccgtc ccgcgtccgt
     4141 tgagacgcgc ggcaagcaca tcgaaagacg gcggcaggat aaagatgccg acagcttcag
     4201 gcagcgcatc tcgaacctgc gccgcgccct gaacgtcgat ttccaaaatc acgtcataac
     4261 ctgccgccgc caacgcattc acgccctccg cgcttgtgcc gtaatagttg ccgaatacgt
     4321 ctgcgtattc caaaaaagcc tcctgtgcga taagcgactc aaactcttct ttggaaacaa
     4381 agtgataatg tacgccgttt gcttcgcctt cacgcggcgg gcgcgtcgtg tgcgacacgg
     4441 aaacgcgcaa accgttatgg tttgccaaca gccgcgacac cagcgtggtt ttgcccgtgc
     4501 cggaagcggc cgaaatgata aagatgttgc cttttcgata agcggacata ttttttacct
     4561 gtatattttc cagccgattg tatcacaatg gacacccagt ttcctatttg ccgatgccca
     4621 tattttgccg ctattgtttt gattgatttg gcaagcggca ggctgacggc tacaatatgg
     4681 cgttaaaaac atcaaacttg gaacacgcaa tgctggttca tcccgaagct atgagtgtcg
     4741 gcgcgcttgc cgacaaaatc cgcaaaatcg aaaactggcc gcaaaaaggc atcttattcc
     4801 acgacatcac gcccgtcctg caaagtgcgg aatacttccg ccttttagtt gatttattgg
     4861 tttaccgcta tatggatcag aaaatcgaca tcgttgccgg tttggacgcg cgcggcttca
     4921 ttatcggcgc ggcactcgcc taccatctca acgtcggttt tgtccccatc cgcaaaaaag
     4981 gcaagctgcc ttttgaaacc gtatcgcaaa gctacgcgct cgaatacggg gaagctgcgg
     5041 tggaaatcca caccgatgcc gtcaaacccg gttcgcgcgt gctgctggtc gatgatttgg
     5101 ttgccacggg cggcacaatg cttgccgggc tggaactgat ccgcaaactc ggcggagaaa
     5161 ttgtcgaagc cgccgccatt ttggaattta ccgaccttca aggcggcaag aatatccgtg
     5221 caagcggcgc gcccttattt accctgcttc aaaacgaagg ctgtatgaag ggctgaaaac
     5281 cgaccctgcc gtctgaaacc ggcagggttg ttatgatgcg ttcaaatcac gcccaaatct
     5341 tgcaagcccc tcaacacgcc gtcttcatca acgccggggc aaacatattt cgccgcctct
     5401 ttcactgcct gttccccgtt acccattgcc acaccgaacc cgacttcaga cagcatttcc
     5461 acatcgttca aaccgtcgcc gaacgccatc acgtccgcca tttccaaacc caatgcttca
     5521 accacgcttc tgatgccgtc tgttttcgac attcccgcag gcagcagatc gaccgcttcc
     5581 tcgtgccagc gcaccgtttt caagccttcc cgttccacaa tatccgacca gggcggcatt
     5641 tcgttttcct ccgcaaacac cagcatctga tacaccggtt tgcttgaaaa ataatcctta
     5701 tcggcaaaaa aatcgctggc gatatgctgc aaggcgcggc acacgcattc cgacagcgcg
     5761 gacacggcga tcccctctcc gccgacaaac gcataatcca tgcccaagcc atccaaatgc
     5821 gcgcaaaccc tgcccatcaa accggcatcc atcggtactt cgcgcacagt ttttccgtgc
     5881 agcagcgcaa actgtccgtt tatcgttacc acggcatcca tgcccgtttc cgccatcatg
     5941 tccctgacct tttcgggaat cgtcgccaaa gaccgccccg ttgccaacgc cgtcaatatg
     6001 cctttgccgc gcaaagccgc caccgccgtt ttcacggaag gacgcaaggt atccgtatat
     6061 ttgcggtaca gcgtatcgtc aatatcgaaa aacacgattt taggattcat cacattctct
     6121 ctcgcattca aactgccgca ttgtatccca atcaggcaaa tacttgataa atccttataa
     6181 atttcccggc aaaattgacc gcgaacacga aaaggcggat aatccgccca tcctcaaacc
     6241 cttttcagac ggcatttgca gcaatgccgt ctgaaacatt tttacaaagc acacaaatca
     6301 tgtttcaaca cacaggacga cacataaagc gtcgccctat gtgttgccct gattcggaag
     6361 gggttacgcc cctcccaaat aaagtctgat tctaccaccc taaagggcgg ggtttcaacc
     6421 gaaaaggaaa cacgatgaaa gcacccgaac tcttattgcc cgccggcgga ttggaaagaa
     6481 tgcgcaccgc ctacgactac ggcgcagatg ccgtttacgc cggcagcccg cgttattccc
     6541 tgcgcgcccg caacaacgaa tttgccaaac ttgatgtttt agaacaaggc attaaagaag
     6601 cgcacgagcg caacaaaaaa ttctttttga ccgtcaacac cctgccgcac aattccaaac
     6661 tcaaaacctt tgtttccgat atggagccgc tgattgcgat gaagcccgac gcgctgatta
     6721 tggcggatcc gggcttgatt atgaccgtgc gcgaaaaatg gccggaaatg ccgatccacc
     6781 tgtccgtaca ggcgaacacc accaactact ggggcgtgaa attctggcag aacatcggcg
     6841 tcgaacgcat tattctgtca cgcgaattga gtatggaaga aatcgccgaa atccgccaac
     6901 aatgccccga catcgaactc gaagtcttca tccacggcgc attgtgcatc gcctactccg
     6961 gccgttgcct attgtcgggc tatttcaacc accgcgaccc caaccaaggc acttgcacca
     7021 actcttgccg ttgggattac aaagtccaca acgctacgga aagcgatgcg ggcgatgccc
     7081 agcttctgca aggttttaac tttgaaaaag cccaagaaga agccaaccaa aacttcgaag
     7141 gcatcaacgg tcagaaacgc catccctacg ccgacaaggt tttcctgatt gaagaatcca
     7201 accgtccggg cgagatgatg ccgattatgg aagacgaaca cggcacctac atcatgaact
     7261 ccaaagacct tcgtggcatt gaagtggttg agaaactcgc caaaatcggc gtggacagcc
     7321 tcaaagtcga aggccgcacc aagtcgctct attatgtcgc acgcgtcgcc caatcctacc
     7381 gcaaagcgat tgacgatgcc gtcgcaggcc gtccgtttga ttacagcctg ttgagcgaac
     7441 tcgaaggcct tgccaaccgc ggctacacca gcggcttcct cgaacgccac cacactcagg
     7501 attatcaaaa ctacctgacc ggccattcca ccgccaaaca aagccaatac gtcggacacg
     7561 ttaccgaaat cgacaaaaac ggctgggcga ccatcgaagt taaaaaccgc ttcgccgtcg
     7621 gcgattcact cgaaatcatc cacccgaacg gcaaccaaac catcaaattg gaacaaatgg
     7681 cccgaaaagg ccagcctgtc gatgtcgcac ccggcaacgg cattcaggtc aaaatccccg
     7741 atatgcaggg caaagaaaaa gccctcatcg cacgcgtgtt gaacccctga gccacaatgc
     7801 cgtctgaacc ccgttttcag acggcatttt gttgaatctt acggtacaat ggcaatgatt
     7861 tcccaccgca agcaaggata tccgacatga aaaaaaatcc tcatactctc cgccgccgcc
     7921 ctgatgctgg cagtctgcca atccggcagc gcacctaaaa acgcgcacac ggcatccgcc
     7981 ggcacaccga ccggagaatc atgcaaagtc atccgggacg acaccgcagg aagcggcaaa
     8041 aacatcattt accgttgctg caacggtcag gcggcaataa atgcagccat tgcagacggc
     8101 gtattggaat cagggattcc cgtcagcttc ggcggcgggg gcgtaccggt tgcaaacggc
     8161 aggaatctgg ttacacggca agcagcaaac ggcgttggca aatccgactc cgaggcctgc
     8221 gaacacgccc taatcaatgc cgcccgcaaa ttccagcaga ccgccggcaa actcggcggg
     8281 cgcggcgtta ccggcttcca cagctacttc gacaagcagc cgctgcaagg cggacaatac
     8341 gactgtcagg caggctcgtt ccacgtccgt gtggtcatgc gcgccaatgt cgtccgttaa
     8401 catatcggca atcaaaaaat taggctgtgt tttagtaatc tgttgatttc aattatttgc
     8461 aaaggaaaag acaattattt tttcggttag gaagagacct atcctattga atacatttaa
     8521 gttaaatacg tctatcaaca ctatattata gtggattaac aaaaatcagg acaaggcgac
     8581 gaag
//
LOCUS       DAWGJV010000086         8562 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWGJV010000086 DAWGJV010000000
VERSION     DAWGJV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.086"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(77..595)
                     /locus_tag="WHQ94_001385"
     CDS             complement(77..595)
                     /locus_tag="WHQ94_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ2128649.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(615..2000)
                     /locus_tag="WHQ94_001386"
     CDS             complement(615..2000)
                     /locus_tag="WHQ94_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ2128650.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KTIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2239..3780
                     /locus_tag="WHQ94_001387"
     CDS             2239..3780
                     /locus_tag="WHQ94_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ2128651.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAEVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPICVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            3809..4408
                     /locus_tag="WHQ94_001388"
     CDS             3809..4408
                     /locus_tag="WHQ94_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ2128652.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4405..5010
                     /gene="nadD"
                     /locus_tag="WHQ94_001389"
     CDS             4405..5010
                     /gene="nadD"
                     /locus_tag="WHQ94_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ2128653.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRRGETYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5069..5455
                     /gene="rsfS"
                     /locus_tag="WHQ94_001390"
     CDS             5069..5455
                     /gene="rsfS"
                     /locus_tag="WHQ94_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ2128654.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5509..5979
                     /gene="rlmH"
                     /locus_tag="WHQ94_001391"
     CDS             5509..5979
                     /gene="rlmH"
                     /locus_tag="WHQ94_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ2128655.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            6052..6318
                     /locus_tag="WHQ94_001392"
     CDS             6052..6318
                     /locus_tag="WHQ94_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ2128656.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6475..7158
                     /locus_tag="WHQ94_001393"
     CDS             6475..7158
                     /locus_tag="WHQ94_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ2128657.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7282..7794
                     /gene="coaD"
                     /locus_tag="WHQ94_001394"
     CDS             7282..7794
                     /gene="coaD"
                     /locus_tag="WHQ94_001394"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ2128658.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7826..8479
                     /locus_tag="WHQ94_001395"
     CDS             7826..8479
                     /locus_tag="WHQ94_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ2128659.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1852 a         2273 c         2507 g         1930 t
ORIGIN      
        1 aatgtaatcc attctgttcc cgatgtttat gccgtctgaa acccatcctg tgtcgggctt
       61 cagacggcat attgcttcaa agcaggtttt ccgaggcaac ccagttcaga atatcggctt
      121 cgaccgcttc gggcgtgccg gggttggcga ttttgacccc gtattcgcct tcgcccagtt
      181 cctcgaggat ttccagttgc gaatcgagca tccctgcttt catataatgt cctttgcgcg
      241 acatcatgcg ctcgaggttg atgtcttgcg gcggactgag gtggatgaag gcagctttgc
      301 cttcggctcc gcgcagaatg tcgcggtagc cgcgtttgag ggcggaacag gttacgatgg
      361 tgtggttcgc accgttttgc gcctgtttcg tcatccagtc gcgcagattg cccaaccacg
      421 gatagcggtc ttcatcggtc agcggaatac ccgcgcccat cttgtcgcgg ttggcttggg
      481 tgtggaactc gtcgccttcg gcatagggac attgaccgag gtgtttctgc agggacagcg
      541 cggcggtggt cttgccgcag ccgcatacgc ccataacgac aaaatgcgta gtcatttact
      601 atcctttccg tctgtcagac gatggcgaac agcagtgcgg acaaggcaaa gccgatgagt
      661 gcgatgaggg tttggttgac cgtccaggtt ttcagcgtgg tcggtacgtc catgtccaag
      721 aggcggccga ccagccagaa gccggagtcg ttgaagtggc tgcaaccgac cgaacctgcc
      781 gccgttgcca atacgataca ggcgagctgc cagtcggtaa agccggcggc ggcaacggca
      841 ggagccatca gcgcggcggc ggtggtcagg gcgacggttg ccgaaccttg cgcgatacgc
      901 agtgccaagg cgacaaggaa acagcccaaa aggacgggaa tgcccaaatc cgccatgctg
      961 tcggcgagtg ccttgccgat gccggaagcg cgcaaaacgc cgccgaacat accgcccgcg
     1021 ccggtaatca gaatcacgga acagacgggg gcgagtgcgc cgtccacggt tttttccaac
     1081 gcgctgccgc tttcgccgcg tttgcgtccc aagacaaaca gtgcgaccaa tacggaaatc
     1141 agaagggcga tcggtgtcga accgattgtt tttgccgtct gaacccaggt ttcgtccgca
     1201 cttacgagtt tttcgctgat gagggccgat acgccggtat tcaggaaaat cagcagcatg
     1261 ggaatcagca tgatggcgac gaccgttcct gctttggcag gttctttcgg caggtcgttg
     1321 tcttgcgtgc cgccgctgag cagttcggga acgggaacat ggatggtgcg ccccaacact
     1381 ttgccgagca tatagccgct gaaataccat gtgatgaagg cggtcggcag acccaaaatc
     1441 aaaacttggc cgatgttcgc gccgtaaaat tcggaagcgg caatcgggcc cggatggggc
     1501 ggcaggaaga cgtgcatgac ggaaaatgcg ccgatggagg caagcgcgaa gggcagtacg
     1561 tcctgtttca tgcgccgtgc ggtggcgaac acgatgggca gcatgacgat tagtccggca
     1621 tcgaagaaaa tcgggaagcc gaaaatcagc gaggcaacgc ccagcgcgaa cggtgcgcgt
     1681 ttttcgccga acatccggat cagcgcgtcc gccagcgact gtgcgccgcc ggatgtttcg
     1741 accaaacgtc cgagcatcgc gcccaggccg accagaagcg ccacgccgcc gagcgtgccg
     1801 ccgaagtttt tgaccagtac gtcgttgaca atgctgcctg tgggcaaacc ggttgccaaa
     1861 gccgtcagca ggctgacgat gaccagtgtc agcagcgcgt ggatgcggaa tttgacgatt
     1921 aaaatcagaa tgaggatgat tgccgccgcc gaaatgccca acagggtttg cgcggacagc
     1981 gtctgtgtcc agccgtccat ttgtaaagcc tttctctgta ataaaagtac aaaattgtca
     2041 ggttttttta agttgtgcga aaacgatagc acaaatcggg cggtaagttg tttgtctgaa
     2101 gttatattcc tgtttatttg aacgatttat ttcagacggc gggaggaagg cggatacggt
     2161 atgtagtcgg attgaaatat acggtatgtg gggcttttgg gtacaatcgc aacatcagtg
     2221 tttcaagtgg ggaaggcgat ggatggacgg cgttgggcgg tatggggtgc ttttgccctg
     2281 ctgccttcgg cttttttggc ggtaatggtc gttgcgcctt tgtgggcggt ggcggcgtat
     2341 gacggtttgg cgtggcgcgc ggtgctgtcg gatgcctata tgctcaaacg tttggcgtgg
     2401 acggtatttc aggcagcggc aacctgtgtg ctggtgctgc ctttgggcgt gcctgtcgcg
     2461 tgggtgctgg cgcggctggc gtttccgggg cgggctttgg tgctgcgcct gctgatgctg
     2521 ccttttgtga tgcccacgtt ggtggcgggc gtgggcgtgc tggctctgtt tggggcggac
     2581 ggcctgttgt ggcgcggacg gcaggatacg ccgtatctgt tgttgtacgg caatgtgttt
     2641 ttcaaccttc ctgtgttggt cagggcggcg tatcaggggt ttgtgcaagt gcctgcggca
     2701 cggcttcaga cggcacggac gttgggcgcg ggggcgtggc ggcggttttg ggacattgaa
     2761 atgcccgttt tgcgcccgtg gcttgccggc ggcgtgtgcc ttgtctttct gtattgtttt
     2821 tccgggttcg ggctggcgct gctgctgggc ggcagccgtt atgccacggt cgaagtggaa
     2881 atttaccagt tggtcatgtt cgaactcgat atggcggttg cttcggtgct ggtgtggctg
     2941 gtgttggggg taacggcggc ggcagggttg ctgtatgcgt ggttcggcag gcgcgcggtt
     3001 tcggataagg cggtttcccc tgtgatgccg tcgccgccgc agtcggtcgg ggaatatgtg
     3061 ctgctggcgt ttgcggcgga ggtgttgtct gtgtgctgcc tgtttccttt gttggcaatt
     3121 gttgtgaaag cgtggtcggc cggcgaatcg tggcgtgtgt taatggaaag tgaaacgtgg
     3181 caggcggtgt ggaatacttt gcgcttctcg gcggcggcgg tgtttgcggc ggcggttttg
     3241 ggtgtggtgt atgcggcggc ggcgcggcgg tcggcgtgga tgcgcgggct gatgtttttg
     3301 ccgtttatgg tgtcgccgat ttgtgtttcg gcgggcgtgc tgctgcttta tccgcagtgg
     3361 acggcttcgt tgccgttgct gctggcgatg tatgcgctgc tggcgtatcc gtttgtggca
     3421 aaagatgttt tgtcggcttg ggacgcactg ccgccggatt acggcagggc ggcggcgggt
     3481 ttgggcgcaa acggctttca gacggcatgc cgcatcacgt tccccctctt gaaaccggcg
     3541 ttgcggcgcg gtctgacttt ggcggcggca acctgcgtgg gcgaatttgc ggcaaccttg
     3601 ttcctgtcgc gtcccgagtg gcagacgctg acgactttga tttatgccta tttggggcgt
     3661 gcgggtgagg ataattatgc gcgggcaatg gtgttgacat tgctgttgtc ggcgttcgcg
     3721 ctgggcattt tcctgctgtt ggacaacggc gaaggcggaa aacagacgga aacgttataa
     3781 tgtgaaccct ttttcagagg acggcaaaat gagcgagttg gataacatcc ttgcccataa
     3841 ccggcagttt gtcgagtcgg gcgaatatga aaaatacttt accgacaaat accccgaacg
     3901 cgggctggca gttttgtcct gtatggatgc gcggattatc gggctgctgc ccgacgcgtt
     3961 gggtttgaaa aacggcgatg ccaagctgat taaaaatgcc ggcgcgctgg ttacgcaccc
     4021 gtggggttcg gtgatgcgga gtcttttggt tgccgtgttt gaattgaagg tcagagagat
     4081 tatggtcatc gcccatcacg attgcggtat gcaggggctg aatgccgaag aattcctcgg
     4141 gcgcgtccgg gaaagccaga ttcccgaaga ccgtatcgaa accctgcgtt atgccggtat
     4201 cgacctcgac ggctggctga ccggtttcga caacgtcgaa gacagtgtgc gccacacggt
     4261 ggaccttatc cgtaaccatc cgctgatgcc gcgccatatc gccgttcacg gactggtcat
     4321 ccatcccgtt accggcaaac tgacgctggt tgtggacggc agtgtttcag acggcatgga
     4381 cttatcggaa ggaatggaaa catcatgaag aaaatcggac tgttcggcgg cactttcgac
     4441 ccgatacaca acgggcatct ccacatcgcc cgcgcctttg ccgacgaaat cggcttggac
     4501 gcggttgttt tcctgccgac aggcggcccg tatcacaaag acgccgcctc cgcttccgcc
     4561 gccgaccgcc ttgccatggt cgaattggcg acggcagaag atgcgcgttt tgccgtcagc
     4621 gactgcgaca tcgtccgccg gggcgagact tacactttcg acaccgtcca aattttccgc
     4681 cagcagttcc cctcggcgca actctggtgg ctgatgggca gcgacagcct gatgaagctg
     4741 cacacatgga aaaaatggca gatgctcgtg cgcgaaacca atatcgccgt cgccatgcgg
     4801 cagggcgaca gcctgcacca aaccccgcgc gaactgcacg cgtggctggg caatgcgctt
     4861 caggacggca gcgtccgcat cttgtccgcc ccgatgcata atgtgtcgtc aacggaaatc
     4921 cgccgcaacc ttgccggcca aggcgtttca gacggcatcc cgcctgccgc cgcacgctac
     4981 atccgcgaac acggtttgta tgaaaaataa agtcaaatca gtaagaaacg gctataatgc
     5041 cgtctgaaaa catcccgtta ggaacataat gaacgaacaa gaactgcaag acctgcaaaa
     5101 aatggtcggg gtcgccgtca acgccctcga agacatcaaa gccaaagaca tttccgttct
     5161 cgaaacgcaa gataaaactt cgctgtttgc cagaatgatt atcgccagcg gcgacagtac
     5221 gcgccaagtc aaagcactgg ccaacaacgt tgccgtcgat ttgaaagaag ccggttttga
     5281 aatcctcagt accgaaggcg acagcggcga atggacgttg gttgatgcag gagacctcgt
     5341 tgtccacgtt atgctccctg ccgtgcgcga cttctacgac atcgacaccc tttggggcgg
     5401 cgagaaaccg agtttccacg ccggaatgca gaagccgtgg cacgcggcag actgattccc
     5461 gatgccgtct gaatgttcag acggcatttt cctgtaaggg agaaagcatt gaacattacc
     5521 gttttggcag tcggcaccaa aatgccgcgc tgggttgatg aggccgtcgc cgaatacgcc
     5581 aaacgcttcg gacgcgacgt cgcctacgca ctcaaagaaa tcaaacccga aaaacgcggc
     5641 gcgggcgtga atgccgccca aggtatggcg gcggaagaaa aacgcatcct tgaagccgtt
     5701 ccgcaaggcg cgttccttgt cgttcttgac gaacgcggca aagcgccgac ctccgtcgag
     5761 ctggcggaac acctcaaaag ctggcggcaa aacggcgaac acgtctgctt cgtcatcggc
     5821 ggcgcggacg gcatgaccga ccgcctcaaa caacaagccc gcatgatgat gcgcctgtcc
     5881 agcctcaccc tgccgcacgg catggtgcgc gtccttctga ccgagcagct ctaccgggcc
     5941 gtttccatcc tgcacaacca tccttatcat cgggaataag aaggcttttg tccgcccccc
     6001 taatcggtta aaatcacccc gttatttctg aacacagcaa aggaatccgc tatggcacgc
     6061 atggtattct gcgtcaagct caacaaagaa gccgaaggca tgaaatttcc gccgcttccc
     6121 aacgaattgg gcaagcgcat ttttgaaaac gtatcgcaag aagcgtgggc ggcgtggacg
     6181 cgccaccaaa ccatgctgat taacgaaaac cgcttaagcc ttgccgatcc gcgcgcgcgc
     6241 gaatacctgg ctcagcagat ggagcagtat ttcttcggcg acggcgcgga tgccgttcag
     6301 ggatacgttc cgcaataacg gttttccgtt ttgaacacag gctgtccgaa actgcttcag
     6361 acggccttta aaatacgctg gacaacttta tcttcacgca accggacaaa ttttgacatt
     6421 gggcaatgtt aaaatccccc attattccca accgcgcttt caggagcaga tgatatgcaa
     6481 cacgacgttt acgactacac cgcgcatacg gtttctaaaa acaccgtcct gcagaaaacc
     6541 taccgcctgc tcggattttc attcattccg gcagccgcag gcgcggcact tgccgccaat
     6601 gccggtttca atttttacgc cgccttcggt tcgcgctgga tagggtttgc cgtcgtgttg
     6661 gcgtttttct acggtatgat ccacttcatc gagaaaaacc gttacagcaa taccggcgtt
     6721 accctgctga tggtattcac attcggtatg ggcgtattga tcggccccgt gctgcaatac
     6781 gcgctccata ttgccgacgg tgcgaaaatc gtcggcattg ccgccgcgat gaccgccgcc
     6841 gtctttttaa cgatgtccgc cttggcgcgc cgaacccggc tcgatatgaa cgcgctcgga
     6901 cgcttcctga ccgtaggtgc ggtcattctg atggtcgccg tggttgccaa tctgtttttg
     6961 ggtattcccg cactcgccct gaccatttcc gccggttttg tcttgttcag ttccttaatg
     7021 attatgtggc aggtacgcac cgtcatcgac ggcggcgaag acagccacat cagcgcggca
     7081 ctgacactgt ttatctcgct ttacaacatc ttcagcagcc tgttgaacat cctgttgtct
     7141 ttaaacggcg aggattgatg ccggcaaaac gccgtccgac tatgccgtct gaaaatgctt
     7201 cagacggcat tttactttgg gcatacaata cggatgcaca cgcaccaaca cacagccgac
     7261 tatgaagccg ggaggaatca aatgttaccg aatacaccgc gccgcgccgt ttatgccggc
     7321 agtttcgatc cgcccacatt ggggcatctg tggatgatac ggcaggcgca atctatgttt
     7381 gacgaactca tcgtcgccat cggcattaac cccgacaaac gcagcaccta taccgtcgct
     7441 gaaaggcagg atatgttgtg cgatattact aaaatgtttc ccaacgtcag aaccgatgta
     7501 tttgaaaacc gatttctggt gcattacgcc cgtgaggtag atgcaggatt catcgtgcgc
     7561 ggcatccgtt ctgcttcgga ttacgaatac gaacgttcca tgcgccatat caacagcgac
     7621 ctcgcccccg aaatatccac cgtattcctc atgccgccgc gcgaaatcgc cgaagtgtcg
     7681 tccactatgg tcaaaggact ggtcgggccg gaaggctgga cggaaacgat ccaccgctac
     7741 gtgccgcaag ctgtgtacga aaaaatcctt gccgaacatc aacacgaaaa ttgaaccctt
     7801 ctatttttgc aaggcatata tggatatgtt ggaaattttg ttccgccatc aagacttcgt
     7861 cgccatcaac aaaccgggcg gcatatccgt ccaccaagac agcggcgaga ccggactggc
     7921 ccgaacactc gccatacagt tgggcgtaga gcgtgtgtgg ctgctgcacc gattggacaa
     7981 acagaccagc ggcattttac tgtttgccct caacagagaa agcgcgtccg cccttagcgg
     8041 acagtttgcc ggcaaaagca tcaaaaaaac ctatttggca ctatccgacc gcaaaccgtc
     8101 caaaaagcag ggttggatta aaggcggaat ggaaaaatcc agacgcggaa tgtggaagtt
     8161 gacgcgcaat acagaaaata tcgccgttac ccgattccac agcatcagta tcgccgaaaa
     8221 actgcggctg ttcatcctgg aaccgcatac gggcaaaacg caccaattga gagtggcgat
     8281 gaaaagtttg ggcagtccga ttctgggcga cagcttatac ggcggaacag aatccgaaac
     8341 catgtttctg tatgcatgga aaatacaatt tgcctaccaa aaccgacaaa tcgaaattgt
     8401 tgcaccctta aaaaacgaat ggcagactga aaatatctct catgcgctgg aagaatttta
     8461 tatggcaaaa gcaggttaat aaatataaat taatttataa acaatatgat aaatattttt
     8521 ataaaaattt ttcaagaaaa cggttgactg caaatctctt ca
//
LOCUS       DAWGJV010000087         8493 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWGJV010000087 DAWGJV010000000
VERSION     DAWGJV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.087"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WHQ94_001396"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WHQ94_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ2128660.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKINGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1672..2055)
                     /locus_tag="WHQ94_001397"
     CDS             complement(1672..2055)
                     /locus_tag="WHQ94_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ2128661.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFVCTVGLFVAAA"
     gene            complement(2059..3600)
                     /locus_tag="WHQ94_001398"
     CDS             complement(2059..3600)
                     /locus_tag="WHQ94_001398"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ2128662.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTKPGELFVTDNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTSSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3890..4723
                     /gene="serB"
                     /locus_tag="WHQ94_001399"
     CDS             3890..4723
                     /gene="serB"
                     /locus_tag="WHQ94_001399"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ2128663.1"
                     /translation="MPHALVLQFPSAEVLPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENILKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDILMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            4789..6012
                     /locus_tag="WHQ94_001400"
     CDS             4789..6012
                     /locus_tag="WHQ94_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ2128664.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     STSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIRIILLAVCMFALIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQYALELAAVKWPVVLMALAAGAYGGLITPSMMLGSTVS
                     FAAAAAWNSVFPAMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6172..7752
                     /gene="purH"
                     /locus_tag="WHQ94_001401"
     CDS             6172..7752
                     /gene="purH"
                     /locus_tag="WHQ94_001401"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ2128665.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAEME
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIYPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRIGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
     gene            8365..>8493
                     /locus_tag="WHQ94_001402"
     CDS             8365..>8493
                     /locus_tag="WHQ94_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ2128666.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKH"
BASE COUNT         1908 a         2304 c         2373 g         1908 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatctacg
      241 gttttcttcg catctccgaa acacatcacg ctgttttcgt tgaagaacag tgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccac
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcggggttg
      421 acggtgtcgt tcgcaccgat gaccaagacc acatcggttt cggggaagtc gtcgttgatt
      481 tcgtccattt ccaaaacgat gtcgtagggg actttggctt cggcgagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gtacttcggt gccgttttta
      601 cgtaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacatt gtttgcgcct ttcagcattt cggcaatatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagata atgtgccgct gtcgctgccg
      781 aaaccaccgg caattaccga gacaaacgag cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccagcgcg ccggtaacga tgagcaggtc gttggagagc
      901 atgaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctgccg tcaatgccga caaatacgag cagcaacaca
     1081 aacgatacgg caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgat tttgccgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgcttgga cagccccgcc agcgaaaaga taaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 tttcaatgcc gtctgaaaaa ttgacgttcg tgttttcaga cggcatctgt ttcaagcagc
     1681 cgcgacaaac agcccgacgg tgcagacaaa tccgcccgcc cacatcagcg agcgcaatgc
     1741 tgctttgtcg gcgatgtagc accagataaa ggcgaggcgg aacaggatga acaggccggc
     1801 aagcgtgttg acggttgctt gtccggcatt gccggttgca tgtgccgtca aaacggcggc
     1861 agcaaacggt gcaaaggctt caaaaccgtt ttgctgcgcg gcgtgggcac gggcggctgt
     1921 gccttgcgtg cgcgccagaa aatcgcgcgg attgtggttg tctttaaacc ggaatccgcc
     1981 cgcttttttg gcatacgccg cacaaaaaag cggcaatagg taggcaatca gaatacacca
     2041 ataggcgaaa gtcatggctt accctttctt aaacatattc agcatacgcc gtgttaccgc
     2101 aaagccgccg aagatgttga tgctggcaat caggatggcg atgaaggcaa gcagggtaac
     2161 gaagccgttg ccttggctga tttgcagcag cgcgccgacg acgatgatgc cggagatggc
     2221 gttggtcacg gacatcagcg gggtatgcag cgagtggctg acgttccaga cgacgtagta
     2281 gccgatgacg caggcgagga cgaacacgat gaagtggttc aaaaatgctg cgggcgcgac
     2341 cgcgccgacc cacagcacca acacggcggc gatgacggcg ggcgcgagtt tcttccacag
     2401 gggaacgggt ttcggctccg gcttggcggc aggcgcggct ttttcagacg acgtttgctg
     2461 cggctgggcg gaaacttgaa tcggcggagg cgggaaggtg atttcgccgt cgcgggtaac
     2521 ggtcatgttg cggataatca cgtcttcgaa gtccagcgtg atttcgccgt ctttgttcgg
     2581 gcttaacagc ttggtcaggt tcaccaagtt ggtggcgtaa agctgggaag actgtccggc
     2641 aaggcggttt gccatgtcgg tgtagccgat gattttcacg ccgttgtcgg ttacgaacaa
     2701 ttcgcccggt ttggtgagtt cgcagttgcc gcccgtcgcc gccgccaaat cgacgatgac
     2761 ggagccggat ttcatgcttt ccaccatttc tttggtaatc agcttgggcg cgggtttgcc
     2821 cggaatggcg gcggtagtga tgatgatgtc cacttctttt gcctgctcgg caaagagctt
     2881 catctcggcg gcgataaatt cgtcgctcat cactttggcg tagccgtcgc cgctgccgcc
     2941 cgattcttgc gggaagtcga gtttcaggaa cttgccgccc atcgattcga tttgttccgc
     3001 cacttccaag cgggtatcga acgcgcgtac cactgcgccg agcgagtttg ccgtaccgat
     3061 cgccgccaaa cccgccacac ctgcaccaat caccaaaacc tgcgcgggcg gcactttgcc
     3121 ggcggcggta atttgaccgg tgaagaaacg gccgaaggcg ttggcggctt caatcacggc
     3181 gcggtagccg ctgatgtttg ccattgaaga caaagcgtcc aaagcctgcg cgcgcgaaat
     3241 gcggggcacc atatccatcg ccagcgcgtt cactttcttg gcgcgcaagg cttcgaccaa
     3301 agcctcgttt tggcgcggcc acaggaagct gacgatggtt tgaccttcgt tcaaaagcgg
     3361 cagttcctgt tcggacggcg cgttgacctt ataaatcaaa gggcaggccc aaaccgccgc
     3421 tttgtcggca acggttgcgc ctgctgtttg gtaagcggca tcgtccaaac ttgccgccaa
     3481 acctgcaccg ctttcgacaa cggtttcaaa gcccagtttg cccaacaggg caacggtggc
     3541 gggcgtgcag gcgacacggg tttcgccgga taatgactcg cgtgggatac cgattttcat
     3601 ctctgaatcc tttttcgggt tgtttatatg tatcgtgggt taaatttaaa tcgggacagg
     3661 gcggagcaac gccgtaccgg tttaaagccg actcacttca aatgttaata tattttagat
     3721 aatcccctta taacgaattt tcatcaggct ggcaatagtt gcggcatttt cccgtgttgt
     3781 ccgacacata ctgccgtttg cgtttgaagt cattttccga ttgcagtccc gcccgccgta
     3841 acattctttg ccgtttcact atataatccg cattttttga gccgccgtta tgccgcacgc
     3901 cctcgtcctc caatttccct ccgccgaagt cctgccttcc gacttcccct cacgcctgcc
     3961 cgaacctgat tgcgccgatg aaaagcgtat gcgttttatc gttgaagaag ggttttcttt
     4021 aagcgaaaaa gacgcggcgt tgcttggcag ccgtcaaatc gaccacgccg tgttgccgga
     4081 tatggatttc ggcgaactcg gtttgattgt cagcgatatg gattcgacgc tgattaccat
     4141 cgaatgcgtc gatgaaattg cggcaggcgt gggtttaaaa gacaaagtag cggaaattac
     4201 cgagcgttcg atgcgcggcg aactcgattt cgaacagtct ttacgcagcc gcgtcgcgct
     4261 gttggcggga ttggacgaac gggttttggc ggatgtttat gaaaacattt tgaagctctc
     4321 gcccggtgcg gaatttttgt tggacgaatg caaaaggcac gatgtgaaat tcctgctggt
     4381 gtcgggcggc ttcacgtttt ttaccgaaag gctgcaacaa cgcctcggct tcgaatacca
     4441 gcacgccaat gttttggaaa ttgaaaacgg caggctgacc ggccgtctga aaggtagaat
     4501 catcgacgcg caggcaaagg cagatttatt gctcgaatac cgcagccgcc tcggattgca
     4561 gccacatcag gtgttggcga tgggcgacgg tgcgaacgat attctgatgc tcaaagaaac
     4621 gggcataggc gtggcttacc gtgccaaacc gaaagtgcgg gcagccgccg atgtctgtat
     4681 caacttcggc ggtttggagc gtgtacgcgg cctgttcagg taggcggata ggaaacggat
     4741 gtcgtctgaa aaacgtgttc agacggcatt tgaacggcag gaacgacagt gggacgcaga
     4801 aaactttggt ttgccctggc agcagcaggc gttatcggcg gtttggtcgg cattgtgctg
     4861 acggaactga tgcacttcat acagcatacg gcatacggtt atggcgcgga cggtgcgtac
     4921 atctcgttcc gcgaaggtgt ggcccgtgct tcggaagggc ggcgtttagg cgtattgatt
     4981 ctgtgcggta tgttggcagg cggcggctgg tggttgctca aacgtttcgg taagccgcaa
     5041 atcgaaatca aagccgcctt gaagcagccg ttgcaggggc tgccgtttct gacgacggtt
     5101 ttccatgttc tgctgcaaat cataacggtc ggactcggtt caccgctcgg acgggaagtc
     5161 gccccgcgtg aaatgactgc cgcgttcgct tttgccggcg gcaggcggtt gggtttggat
     5221 gaagacgaaa tgcggctgct gattgcttgc gcttcgggtg cgggtttggc ggccgtgtat
     5281 aacgtgccgc tcgcctccac actttttatc ctcgaagcca tgctgggcgt gtggacgcag
     5341 caagccgtcg ccgccgcatt gctgacttcc gtcatcgcca ccgccgtcgc gcgcatcggc
     5401 ttaggcgacg tgcagcaata tcatccggct aaccttaccg tcagcacctc attactttgg
     5461 ttttccgccg tcatcggccc gatactgggc gcaaccgccg tctttttcca gcgcacagcc
     5521 caaaagtttc cctttatcaa gcgcgacaat attagaatca ttctcttggc agtctgcatg
     5581 tttgcgctca tcggcgtgat ttccgtttgg tttcccgaag ttttgggcaa cggcaaagca
     5641 ggcaaccaac tgacttttag tggtttgacc ggttggcaat acgctttgga acttgccgcc
     5701 gtcaaatggc cggtcgtctt gatggcgctg gcggcaggcg catacggcgg actgattacg
     5761 ccgtcgatga tgcttggcag caccgtttct tttgctgccg ccgccgcgtg gaacagtgtt
     5821 ttccccgcaa tgccgtctga aagcgcggca gtcgtcggag cggcggtgtt tctcggcgta
     5881 tcgctcaaaa tgccgctgac cgccattgtg ttcgtattag aactgaccta cgcgccgctg
     5941 tccctgctgc tgcctttgtg catcggcatg gcgggggcat tgggaacatc gggaaaaatg
     6001 ggcttcaaat gaatgtcgac aaatgccgtc tgaaaggttt tcagacggca tttctttgta
     6061 aattgtcaac aatatctgca aaaatctact gccaaaaaac tgaaaatagc ggataatacc
     6121 gccccgaaaa agttcccctt tccccatcca actgtcagaa aaggaagccc aatgtccgtc
     6181 atcaaacgtg ccctgatcag cctgtccgac aaggcaggcg cggtcgaatt tgcccaaacc
     6241 ctgcacaagc tcggtgtcga aatcctctcc accggcggca cggcgaaact gcttgccgat
     6301 gcgggcgttc ccgtgattga agtcgccgac tataccggct tccccgaaat gctcgacggc
     6361 cgcgtgaaaa cgctgcatcc gaaaatccac ggcggtattt tgggtcggcg cgatttggac
     6421 gaacacgttg ccaagatgga agaacacggt atcggcaaca tcgaccttgt gtgtgtcaac
     6481 ctctacccct tcgccgccac cattgccaag cccggctgca cgctggaaga cgcgattgaa
     6541 aacatcgaca tcggcggccc gaccatggtg cgttctgccg cgaaaaactg gaaacacgtc
     6601 gccatcgtta ccgacacagc cgatttcccg gccatagctg ccgaaatgga agccaacaac
     6661 ggcgcattga gcgacaaaac ccgcttcaac ctctcacgca aagcattcag ccataccgcc
     6721 caatacgacg gcatgatttc caactacctg acctcgcttt cagacgacgt cttgagcggc
     6781 cagccccaaa tcggcgaatt cccaagtcgg ttcaaccaaa gctggattaa agtgcaagac
     6841 atgcgctacg gcgaaaatcc gcaccaacgc gccgcgttct accgcgatat ttaccccgcc
     6901 gcaggcagcc tcgctgcata caaacaactg caaggcaaag aattgtccta caacaacatc
     6961 gccgatgccg atgccgcatg ggaagccgtc aaatcctttg acgcaccagc ctgtgtgatt
     7021 gtgaaacacg ccaatccgtg cggcgtcgcc gttgcagccg atacattgac cgcctacaaa
     7081 ctcgcctacg tcaccgacac caccagcgcg ttcggcggca tcatcgcctt caaccgcgaa
     7141 gtggacggcg aaaccgtcaa acagattacc gacaaccagt ttatggaagt cttgatggcg
     7201 ccgaagttta ccgccgaagc cctcgaaatc gccgccgcca agaaaaacgt gcgcgtattg
     7261 gaagtgccgc ttgaggcagg cgcaaaccgc ttcgaactca aacgcatcgg cggcggactg
     7321 ttggtacaaa cccccgacat ccaccgcatc agccgcgccg atttgaaagt cgtctccaaa
     7381 cgccaaccga ccgagcagga atggaacgat ttgctgtttg tctggaacgt cgccaaatac
     7441 gtcaaatcca acgccatcgt cttcggcaaa ggcggtcaaa cctacggcat cggcgcaggt
     7501 caaatgagcc gcgtggacag cactcgcatc gccgctcgca aagcgcaaga tgccaatctc
     7561 gacctcaatg gcgcatgtgc cgcttccgat gctttcttcc ccttccgcga cggcgtagat
     7621 gtcattgccg agcagggcat caaagccatc atccatccgg caggctccat gcgcgatcag
     7681 gaagtcttcg acgcagcgga cgaacacggc atcgccatgg tcgtaaccga cgtgcgccac
     7741 ttccgccatt aacgcagatg aacaaggcaa tgccgtctga agggctttca gacggcattt
     7801 tgcgctattt tgtgaaggta gggatgacgg ttcgggtatt cctgacaggg tggattttca
     7861 aggtgttgta tagggtgtag gaggattcgt aaaaggtggg atgcagggtg tgcttcagcc
     7921 cgctgcatca aaaatttttg gagaaccggc gggagtcggc ggttttggtt tcggcgggga
     7981 cggtggaaat gggtaacatt gacggaatcg acggaagcgg tgggctgaag cccacccttg
     8041 tatattggaa tcactgtatc atagcaacaa accgcccggc cgccacccgc gcccacccaa
     8101 ggcacacaac cgttgcgtag cacagggagc ggcagggcaa cccatcgaca caaccggaca
     8161 gttgccggac aacacaaccg aatgcaaggc aggtttatga tgagtaccca ataccattac
     8221 gcaggtatag tgaattaaat ctaaaccagt acagcgttgg ttcgccttag ctcaaagaga
     8281 acgattctct aaggtgctga agcaccaagt gagtcggttc cgtactatcc gtactgtctg
     8341 cggctcgccg ccttgtcctg atttttgtta attcactata tcgacatcgc caaacgaaac
     8401 ttcgtcatcg ccgtttcgtc tttgtctaaa accaaaaccg aaaccaacaa ccccaaaggt
     8461 atcgcccata ctatcgaata ccttaaaaaa cac
//
LOCUS       DAWGJV010000088         8251 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWGJV010000088 DAWGJV010000000
VERSION     DAWGJV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.088"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(102..662)
                     /locus_tag="WHQ94_001403"
     CDS             complement(102..662)
                     /locus_tag="WHQ94_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ2128667.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1079..2119)
                     /gene="mutY"
                     /locus_tag="WHQ94_001404"
     CDS             complement(1079..2119)
                     /gene="mutY"
                     /locus_tag="WHQ94_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ2128668.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFSG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2282..3323
                     /locus_tag="WHQ94_001405"
                     /pseudo
     CDS             2282..3323
                     /locus_tag="WHQ94_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3346..3565)
                     /locus_tag="WHQ94_001406"
                     /pseudo
     CDS             complement(3346..3565)
                     /locus_tag="WHQ94_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3580..4218)
                     /locus_tag="WHQ94_001407"
     CDS             complement(3580..4218)
                     /locus_tag="WHQ94_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ2128669.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4399..6234)
                     /gene="edd"
                     /locus_tag="WHQ94_001408"
     CDS             complement(4399..6234)
                     /gene="edd"
                     /locus_tag="WHQ94_001408"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ2128670.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6750..8195
                     /gene="zwf"
                     /locus_tag="WHQ94_001409"
     CDS             6750..8195
                     /gene="zwf"
                     /locus_tag="WHQ94_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ2128671.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKAHKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         1931 a         1972 c         2267 g         2081 t
ORIGIN      
        1 aatttaattc actatactgc tgaagtcgtg aattaaatgc taaatatcta gcaaaaaagt
       61 gcggtcaaaa tttaccgcac ttttcgtttc gaacaatcga attatttaat cacgccacat
      121 gccatacgtg ggccgccacc gccaagtgga gctggatgat cggagtgatt atcaccaccc
      181 gtgtggatca taatagagtg accgcgaaca tcatctaaat gtttaagacg tggtgctaaa
      241 acaggatttg ttgctgtgcc atcatgcaat acagttaatg caggtaaatc acctaagtgt
      301 gcatcatctt gccatgggta accatgttgt tttgcacctt taggatccca gtgaccgcct
      361 gcgcctaaac cagctgtcaa tttaccttct ttttcttttg gctcacagct tgggttttca
      421 tggatgtgga aaccatgtaa gccttcgctt aatccttgta aatcaggggt aaacacaaga
      481 ccatagttag attcagtaat agtcactgta cccacatctt tgttaccgtt tactggatca
      541 agttgttgca ctttcacttc aatagaagca cctttaggta tcgtattatg ctcgtgtgct
      601 tgcgcaacac caactgaaca tactgcacta accgctagtg ctaataaggt tttcatattc
      661 attgcaatct cctttattgc agtaaaaact cgcgactatt ttagtcaatt ttgttttaaa
      721 agccgataaa tttttacgaa aattttacat taattgttat ttgcagaaaa ttagaacccg
      781 ttatcacgtc ttggataaat gccaaaacca ttgtgattca gagaattaaa aagaaaaatt
      841 tcggaatcct catccttttc tgaaacctga ttgcggtatc catggctttg tctttgtcat
      901 ttaagtttgt gttcatgttt ttgaccttct ttgcttttta aatccttttt aaattgccga
      961 cttctaccgt tccaaatgtc ccatttgaac attatcccgc cgcctaaaat acgacccaaa
     1021 ttcatcggtt gttcttgctg cgatacctgc aaacaacagt tcaatgggtt tattttgttt
     1081 attctaacct attaccgttt aaataaattt ccaaaggctt gggcaaaccg taatctttca
     1141 aatgcgccgg ctttatccaa atgccgtctg aagggctttc agacggcatt tgtccttcaa
     1201 acggcgtaat catcagcaac cggtgcgtca gccggtgggt cagggcggtt tgttcgtcca
     1261 tatctgccat ggtcagggag aatttggcgg caaagtcgga aagcccgttc aaactttcaa
     1321 aacacggcac gcaatacagc ccgccccaaa tgcctttggc ggggcgtttt tccagcaaaa
     1381 tcgcgccgtt ccggttgcgg ataatcagcc agtaaagcgg cagggtcggt acttcggcgg
     1441 cggttttttt gcgcggcagc tcggcggtgc ggttttgctt tttcgcttcg cagatgtccg
     1501 ccatcgggca ttggtggcac aagggtttcg tccgtttgca cacggtcgcg cccaaatcca
     1561 tcaaaccttg tgtataggca ggcatatcgg cgttttcaga cggcagcagg ctttcggcaa
     1621 gtgtccagag cgagttttca aattttttgt cctgcggatt gccgtcgcgg gcgaacacgc
     1681 ggcagagtac gcgtttgacg ttgccgtcca aaatggtttc gcggcggttg aaggagaagg
     1741 cgcaaatggc ggcggcggtg cttctgccta cgccgcagag ggtttccaag tctttgcgct
     1801 ccgacggaaa cgtgccgctg aattgcctga cgacttgttg cgcggctttg tgcaggttgc
     1861 gcgcgcggct gtaatagccc aagcccgccc acaacgacaa cacttcgtct tgcggcgcgg
     1921 cggcaagcgt ctgaacggtc gggaattttt ctaagaagcg cggatagtag tccaacacgg
     1981 tggcgacttg cgtttgctgg agcatgattt cggaaagcca gacgcaataa gggtttttga
     2041 cctgccaagg gaggtggtgg cgaccgtgtt gtttttgcca gcggatgagc cgttcggaga
     2101 aggggatggg tgtgttcatt aatatcaatc ggtggtttta tttatattta aaacggtatg
     2161 ttattgtata acattgtgaa aataattctt attgacttat tttttgtagg ggcatataac
     2221 tcatataaag aaactttatt gcggtattga aattatttat caacaagcaa ggagtatcag
     2281 catgaaagca atggtttatc acggcgcaaa cgacatccgt tttgaagaaa aaccccgtcc
     2341 gcagattatc gatccgaccg atgcggtggt gaaaatcgtc aaaaccacga tttgcggtac
     2401 cgatttgggt atttggaaag gcaaaaaaac ccgaagtcgc cgacggtcgt attctcggtc
     2461 atgaaggcat cggtattgta gaagaagtcg gcgatgctgt aaaaaacatc aaagtcggcg
     2521 ataaagtcat tatttcatgc gtcagcaaat gctgtacttg cgacaactgc aaaatccaac
     2581 tttattcgca ttgccgcaac ggcggctgga ttttgggcta catgattgac ggcacgcagg
     2641 cagaatacgt ccgcacgcct tatgccgaca acagcctcgt gccgctgccc gacaacgtca
     2701 acgaagaagt cgccctgttg ttgagcgacg ctttgccgac cgcacacgaa atcggcgtgc
     2761 aatacggcga tgtcaaaccc ggcgacaccg tattcatcgc aggtgccgct cctgtcggta
     2821 tgtccgcctt gttgaccgcc caactgtata gccccgccgc cattatcgtg tgcgatatgg
     2881 acgaaaaccg tttgaaactg gcgaaagagt tgggcgcgac ccataccatc agccccgctt
     2941 ccggcgacgt ctccaaacaa gttttcgcca tcgtcggcga agacggcgta gattgtgcca
     3001 tcgaagcggt cggtatccct gcaacttgga atatgtgcca agacatcgtg aaacccggcg
     3061 gccatatcgc cgttgtcggc gtacacggtc aatccgttga tttcaaactg gaaaaactgt
     3121 ggattaaaaa gcttgccatc actaccggcc tggtaaacgc caataccacc gaaatgctga
     3181 tgaaggcaat ttccagcagc tccgtcgatt acaccaaaat gttgacccac cgtttcaaat
     3241 tcagcgaatt ggaaaaagcc tacgacgtgt tcaaacacgc cgccgaaaat caagcgatga
     3301 aagtggtttt ggaagcggat taatcggcgt tgaatcagtt gttttttaag cggatgccgt
     3361 ctgaatgcgt ttcagacggc atttttttca gccgcgtagt atgggctgtc cggctgctgt
     3421 cgaatgaact gaacgttatt tttattatgc ggaaggcgga tgggcatttt aggggcagta
     3481 tgtcggagtg gtgctgccgt gtatgccgac tgcgccgcca agcggcaaaa tgccgtctga
     3541 gactggaaag ggcttcagac ggcattttta cgatgcggtt caggctttgg gtttcaatgc
     3601 cgccgcttct ttggcgagac gggtgatggt gtcccagtct ttgtttttca cggcttcttt
     3661 cggcgtcagc catgagccgc cgacgcacag gacgttgggc agtgccaagt aatcgggcgc
     3721 ggtggcgagg ctgatgccgc ctgtcgggca gaagcgaaca tcggcgtaag ggccgtacag
     3781 ggctttgagc atggctttgc cgccaacgac ttcggcaggg aagagtttga gggtgtcgat
     3841 gccgtgttcc aaagccagtt ggatttcgcc cggggtggca acaccgggaa tcagggggat
     3901 gccgctgttg cggccggctt tggcgaggga ttcatgcagc cccgggctga tggcgaaaac
     3961 cgcgcctgcg tcttcgacgg ctttaagctg ttcgggattg gttaccgtac ctgcgccgac
     4021 gatggcgttg ggcacttctt tggcaatcag gcggatggca tcgaggccga caggggtgcg
     4081 cagggtgatt tcgagggtag ggatgccgcc ttcgacaagg gcgtgggaca aatcgatggc
     4141 ggtgcttaag tcgtcaatcg ccattaccgg cacaactgcg ccggcggtca aaatttcgcg
     4201 gggggtcagt ttggacattt cggttctccg ggtggaatgg ggtattttat taagatggga
     4261 caggtcgtag gtcggattca agaaaccgac atcttggacg gctgtgtcgg attcgagaat
     4321 ccaacctacg tctatcttaa aacacttgaa taatttttca gacggccttc ggtctgaaaa
     4381 ggccgtctga aacgcgcatc aggcaaattc gccgccgaag ctcatcgcgc cggtttcggc
     4441 gctgctggtc atgctgcgga agttggcgaa aagttcgcgg ccgcagcctt gttggttcgc
     4501 gcccaagtcg atgctttcga cttcgcgggc attccattcg gtttcgttaa tcaggacgtt
     4561 gagttcgccg ctaacggagt cgaagcggat caggtcgccg gtacggattt tggcgatgtt
     4621 gccgcccatc agggcttcgg gcgtcatgtg gatggaggcc ggaactttgc cggatgcgcc
     4681 ggacatacga ccgtcggtca gcagtgccac tttgaagccg cggtcttgca ggatgcccaa
     4741 aggcggggtc agtttgtgca attcgggcat accgttggca cgcgggcctt ggtagcggac
     4801 gacgcacaca aaatcgcgtt ccaactcgcc gcgttcaaac gcagccaaca cttcgcgttg
     4861 gtcgttgaac acgatggcag gcgcttcaat aatgcggcag ccttcgcgca cggcggacac
     4921 tttaatcacg ccacgtccga tgttgccttt catcaggcgc agaccgccgt cgggggagaa
     4981 cgggttgtca gctttacgca ggatgtcgtc gttgccgctg gtttcggggg cttcgcgcca
     5041 ttcgagtttg ccgtcgatga ggaaaggctc tttggtgtag tggcgcatac cgtgtccgac
     5101 gacggtatcg acatcgtcgt gcaacaggcc tgcattcagc aattcgcgga taacgaaagg
     5161 cagtccgccc gctgcggtaa agtgattcac gtcggccttg ccgttcggat aaacgcggat
     5221 gagtagtggg atgatggagg aaatttcgtc gaagtcgtcc cagttcaaaa tcacgccggc
     5281 cgcacgcgcc atagcgacga ggtgcatggt gtggttggtc gaaccgccgg ttgccatcag
     5341 gccaatcaag gcgttgataa aggatttttc ggtcaacatt tcgcccaaag gtttaatcgt
     5401 gccgtttttg atgccgcgcg cgaggtgtcc ggcggcgtag cgagtcagcg cttcgcgcag
     5461 gtcggtgtaa gggtggacga aggcggcggc aggcaggtgc acgcccatca tttccatcat
     5521 catttggttg gagtttgccg tgccgtagaa agtgcaggtg cccgggctgt ggtaagaacc
     5581 catttcgctt ttcaaaagtt cgttgcgtcc gaccttgcct tcggcgaaaa gctggcgggt
     5641 gcgggctttt tctttgttgc cgataccgct ggacatcggg cctgcgggga cgaagatacc
     5701 cggaatatga ccgaacgaaa gcgcgcctat catcaaaccc ggaacgattt tgtcgcatac
     5761 gcccataaac aggctgccgt caaacatttg atgcgacagc ccgacggcgg tactcatggc
     5821 aatcacgtcg cgggagaaca gcgacagttc catgccggcg tagccttgcg tgatgccgtc
     5881 gcacatggcg ggcgtgccgc cggcgacttg ggcggtcgcg ccgtttttct gcgcttcgtc
     5941 tttgatttgg tcagggaagt ctttaaacgg ctggtgtgcg gaaaccatgt cgttgtaggc
     6001 ggtgatgatg cctaagttgg ggacggtttc ctgaagcatt tcgattttga tacttttagg
     6061 catggcagca tagccgtgtg ccaagttgct gcagccgagc tggttgcgct ctaagcgtcc
     6121 catttgtttg gcactgcgga tcttcgccag atatttttca cgcgtcggac ggctgcgttc
     6181 aataatgcgc ccggtgattt cggcgagttt ggagtggata ggagtagtgt tcacggtcgg
     6241 tctcctgtcg gaacgggggg tattggggat ttgtttgtgc gtttctgatt tttgtatcgg
     6301 gtattcagac ggtaagtata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc
     6361 cgtattatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
     6421 attttaaaag gtatgccgtc tgaaagtggg aagcgggtgt tattatagta caaaatttgt
     6481 aattttgtta caaaaaaatc agacatcgaa aagaaatttg atttaaatcg cagaatcttt
     6541 cgattgttgt aacggaatag gggaaacctg cccgattttt ggactaggtc aaataaattg
     6601 taaaaatggg aatagacaaa aagtgtagcg gtgtgtagta ttactactca ttatgccgcc
     6661 tgcctttgtt tccgatattg cgatgcagcg tgatggggaa aacaggtttc aaacggcatc
     6721 gaaaaattct ttcctgacga gataatacga tgagtacaca aacaaatttt gatttggtgt
     6781 tgttcggtgc gaccggcgat ttggcgatgc gcaagctgtt gccctgtctg taccaggcgc
     6841 atgttgccgg tctgcttcat cccgaagggc gtattttggg ggtcagccgc agcgagttgg
     6901 ataccgaagg ttttctggcg aaggtggaaa ccagttccaa aatccatgtg aaagaaaatt
     6961 tttccgatga ggcttgggca tcgtttgtcg aacgtttcgc atatctcaaa gtcgatgtta
     7021 cgcagccgga cgattttgcc gctttgggcg atttggtcaa agcgcacaaa gaaaccgaca
     7081 atgtcgtcat ctatctttcc accgcgccga aattcttcgc gcaaacatgt gaaaaccttg
     7141 ccaaaatcgg tttgaacgcc gacaacgtgc gtgtggtttt ggaaaaaccg ttgggtacgg
     7201 atttggcttc ttcccagcaa atcaataccg atgtcgcgcg ttatttcaaa gaagggcaaa
     7261 tttaccgcat cgaccactac cttggcaaag aaagcctgca aaacctgctt gcgctgcgtt
     7321 ttgccaatgt aatgttcgag ccgctgtgga acaataaata catcgaaagc gtgcagttga
     7381 ccatcgccga gcagttgggc gtggaagaac gcggcgagtt ttacgacatt accggcgcgt
     7441 tgcgcgatat ggtgcaaaat cacttgatgc aaatgctgtg catgactgcg atggaagccc
     7501 ccgccagctt ggatgccgac gcggtgcgcg atgaaaaagt caaagtcatc aagtcattga
     7561 agccgctgac cgtcgaatct gtcaatgaaa atgtcgtgcg cggacaatat accgccgcca
     7621 aaggcatgaa cggctatctt gaagaaatca acgttccgca agacagcttt accgaaacct
     7681 acgtcgccat taaagccgaa atcgaaaacg aacgctggaa gggcgttccc ttctacctgc
     7741 gtaccggcaa gcgcatggcg ggcaaagtgg cggaaatcgt tttgaacttc aaagatttga
     7801 acagccatat ttttgaaggc agccgcaccg cgcccaaccg gctcgttatc gagttgcaac
     7861 catatgaatc cgtgcgcctc tatacgcaga tgaaaacccc gggggcagga aataaggtcg
     7921 aaaccgtgcc gctggcaacc gatttgggca aagcattgga aagccgccgc gcggaagctt
     7981 acgagcgcct gctgctggat gtgattaacg gcaaactcgc tttattcaac cgccgcgacg
     8041 aactcgaagc cgcgtgggaa tatgtgatgc cgattttgga aaactggaca aataacacca
     8101 cgccgccgca cggctacggc gcacactcgt gggggcctga agccgcgcgc gaactattgg
     8161 cgcgcgacgg acacaagtgg cacgaagagc agtaatacaa taatgcgttc agacggcatg
     8221 gggtttgaaa tgccgtctga acgtaagtaa a
//
LOCUS       DAWGJV010000089         8215 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWGJV010000089 DAWGJV010000000
VERSION     DAWGJV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.089"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(264..2954)
                     /gene="glnE"
                     /locus_tag="WHQ94_001410"
     CDS             complement(264..2954)
                     /gene="glnE"
                     /locus_tag="WHQ94_001410"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ2128672.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3134..3469)
                     /locus_tag="WHQ94_001411"
     CDS             complement(3134..3469)
                     /locus_tag="WHQ94_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128673.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            complement(3723..4196)
                     /locus_tag="WHQ94_001412"
     CDS             complement(3723..4196)
                     /locus_tag="WHQ94_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ2128674.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4249..4530)
                     /locus_tag="WHQ94_001413"
     CDS             complement(4249..4530)
                     /locus_tag="WHQ94_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128675.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4617..4940)
                     /locus_tag="WHQ94_001414"
     CDS             complement(4617..4940)
                     /locus_tag="WHQ94_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128676.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5273..6280)
                     /locus_tag="WHQ94_001415"
     CDS             complement(5273..6280)
                     /locus_tag="WHQ94_001415"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ2128677.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6512..6748
                     /gene="acpP"
                     /locus_tag="WHQ94_001416"
     CDS             6512..6748
                     /gene="acpP"
                     /locus_tag="WHQ94_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ2128678.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6904..8151
                     /gene="fabF"
                     /locus_tag="WHQ94_001417"
     CDS             6904..8151
                     /gene="fabF"
                     /locus_tag="WHQ94_001417"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ2128679.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1900 a         2160 c         2116 g         2039 t
ORIGIN      
        1 gtagaatgcg ctattttaat ggaaacggcg gggttttaaa cggttcttat ttgtttgttt
       61 tgatttttgc ttgggtaaaa aatcccatca ttcccacaaa aacagaagcc tgaaatcccg
      121 tcattcccgc gaaagaggaa tccggttttt gggtttcagc cattcccgat aaatcgcttt
      181 agctctgccg caaccttaaa acggcacggt acgaaaatac cgtctgaaac ccagattgtc
      241 aggcttcaga cggcattttt tgttcaggcg gttgccgctt cttcgccgaa cacttcccgc
      301 cacaatttcc tgacattgcc gtaatgtgcc aacagttcgc cggttacttc ggtttttgcc
      361 gcgtcgcgca gtttggtgtt gtgctgctgc cggcggtaga agcgataggc ggtgcggctt
      421 tgtccggcga gggttttgtc aatcaaaccg cagtcggcgg agatgtttaa gagggcgatg
      481 ttgccgtagt tgtccaagag ttgcggatac tggcgggcat gggcaagtat cagatattgg
      541 acgataaatt cgacatcgac cacgccaccg cgcgcgtatt tgacgttgct gtcggcaggc
      601 gggtgggtgg ggaacatttt ttcgcgcatt tcgatgattt cgcctgccaa ggcggtttgg
      661 tcgcgttcgg cggtgaggat ttcggtgcgg atgcggtcga aggccgtctg aatttcggac
      721 gtgccgcaga tgaagcgggc gcgggtaagg gattggtgtt cccacgtcca ggcgttttcg
      781 cgctggtatt tttcaaaggc ggcgatgctg tgggcgagga aaccggcgtc gccattaggg
      841 cgcaggcgca ggtcggtttc gtagaggctg cctgcgccag tggcggcgga aagccagttg
      901 gtcaggcgac gggcgaggcg gctgtacacg tcgcctgcgt cggggtgggg atcgtcgtag
      961 aggtagacga ggtcgaggtc ggaggcgtag ccgagttctt taccgccgag tttgccgtag
     1021 ccgacgacgg cgaattgcgg tgtgtcgcgg tgttttttgg gcatgtccgc ccatgcgcac
     1081 agcagggcgg cggcgaggat ggtgtcggcg agggcggaga gttggtcgga gagggattct
     1141 accgtccaca gtccggcgag gtcttggacg gcgagacgga agacttgggc gtgctggaag
     1201 cggcgcaggg tgtccatttg cgcttcagta tcgccgccgc aggctttgag gtcgtctgaa
     1261 agggcggcgg cgagcgcctg ccaatcaaac gcggtatcca aaagctgcgc gctgatgagt
     1321 tcgtccaaca aaatcggata tttgttcaga tacgccgcca cccaagaact ttggcccata
     1381 atctgcgcca gttgcgccaa ggtttgcgga tgttcgttga ggaaggcgag ataggcggat
     1441 cggcggctga tgttttcgag aaaatccaac agccgcatca atgtatcggt cgggttgctt
     1501 tgcgctgccg ccgcctgtac gaacagcggc acaaccgcat cgaaacgcgg ctgggcgtgt
     1561 gcggaaagat ggcggtattt atggccgtgg cggatttggt cgagccttgc ggcgacggtt
     1621 tcggcatcga acccgtgcgc cttcagacgg catcgccgcc cttcttcgtc gggtttgtcc
     1681 tgccatgccc attgccattc gctgttgtct tgcgtttgct cttcgggttc gctcaaaatt
     1741 tcgttgaaca actgattgac tttgttccga tgaacattga gaccgtctga aaaagcggaa
     1801 taactgtcga aacccatgct ttcagcgagc agttgccgct gttcaggcga agtgggcagg
     1861 gtttgggttt gttggtcgtc ccagtattgc aggcggtgtt cgacgtcgcg caggaagcgg
     1921 taggcggcaa gcagggtttc gacgttttca gacggcatga tcccggtttc ggcaagtttc
     1981 ttcagcgttt cctgcgtgcc tttcagttgc agcgcacgca tttggccgcc gcgtatcatc
     2041 tggaaaatct gggcgataaa ttcgacttcg cggatgccgc ccgcgccgag tttgatgttg
     2101 tccgccatgc cttttttgct gacttcgctg cggatttggc ggtgcagctt acgcatcgcc
     2161 tcatacgcgc cgtaatccag atatttgcgg aacacaaagg ggcgcaccag tgctttgatg
     2221 tcgttcggat acggcgtaac cacgcgacct ttgcaccacg cgtagcgttc ccattctcgc
     2281 ccctgtgtaa tcaaatattg ctccagcgcg gtttcgctca ataccaacgc gcccgaatca
     2341 ccgtccggcc gcagccgcat atcgacgcgg aacacttgcc cgtcggcggt aatgtcgttc
     2401 agcaacgcaa tcagtttctg cccgactttg gtgaaaaact cctgattgcc gcgttcgcgc
     2461 ctgccgtcgg tgtcgcccga ttcgggatag acgaaaatca aatcgatgtc ggaagacacg
     2521 ttcaactcat agccgcccgc cttgcccatc gccaccacgc tcaaatgctg cggcgatttg
     2581 gtataacgcc cgatcggcgt gccgtacatg tcccgataat aggcgtaggc aaaatccagc
     2641 gcggtattga cggcaaaatc ggcaaacagc gtaatcgtgc gggttacttc gttcaaatcg
     2701 ctgatgcggt ttatatcgcg cacgataatc tgcgacacca cataacggcg caacccgcgc
     2761 aactgccgcg ccaattcttc ctcgttttct tccgcacgga ttttgtccca gtcggcaaag
     2821 gcttggaaat ccgcttcggt caaaaccttg tccagcatag gcaggaaaat ttcgggcttg
     2881 agtttgccgt tgtcgagctg gcgggcgagg aagagggaat ggcggcgggc ggtgtcgagg
     2941 cggttgtcgg acatttcgga ttccgtttga aaaaatggca ggaagatgat ggcggattca
     3001 cttcaaatca ggacagggcg aggggatgcc gtaccggttt aaagtcaagc cactatatca
     3061 tagcaacctt cgatgccgtc tgaaagccgg ataacgcttc agacggcatc ggattgacag
     3121 gtttaatccg cgcttacgga cgctttttaa atataaataa agaaatctcg tctaacgcat
     3181 cttcggcaaa accttgaaaa tccggatcac cttgttttga gagcaataaa gcaatcactt
     3241 tatcgcaatc gccgatattt tcatgcatcc tggcgagatg tccgaaacag gttaatgaca
     3301 atctggcaat atccaagtct ttatgtttcg acaactccgt caacttctcg gaaatccagg
     3361 ggatttcatc ctcctccgag ctgaaggtca taccgagcat accgtcaata acactctgtt
     3421 tattttcaga ataaatcatc ttttctactt gttccttatc aaccgtcatt ttttaatttt
     3481 tccttttaaa ttaaccaatc ctgctttctt taaagcattt ttgccgggcg gcaggattgt
     3541 tatcaaacat ttgcacccgg tccggcaaaa cgatttcctt atcggtttat cggcaacgat
     3601 gcgggcggtt cgggttttgc cgcttccgga atgcagcccg cccccgcttt caaatcttcc
     3661 gctctaatcc gtttgccgtc tgaaggccgg ataatgcttc agacggcatc ggattgccgg
     3721 ttttaaccct gccctccgcc gttgttcaaa tagtgttccc agtgagaata taaccgggca
     3781 atattttctt cataggtgtg ggcagaagga atataaccgt ctttgggcgg catatacctg
     3841 acaaatgatt ctatttcttc cggataatca aaatctgcat aaattttttc aacttccccc
     3901 aaaggatcct caaattcttc ttttctatgg aatacatcgc ttaagaggac gtacagccaa
     3961 tttttagggg aataatccct catttccgaa gccaaatctt ccagaacagg ctttaattca
     4021 agaatattgg atttgttgat taaagatagt ttgaacacac gttcatcatg atctgaaagc
     4081 gtcatttttc gggcataagc tgccacatca gcccaaccca agtatttatt cccatatccc
     4141 cacagaatat ccttccaaga aagacggact ttttgcgcgg ttaaatcaag gttcattttt
     4201 tactccgatt gcctgccccg tcattccgcc ccttccgatt gacgcctgtt acgaaaagtc
     4261 ttccaaatga cggctgattg cctcttcaac agtcggatgc caatccataa atgaagcctg
     4321 caattcctct aaaaccaact cattgaataa atcatccaaa ttccgccaat ccgttttttc
     4381 atccggatag atgaaatttg ccataacgac agtatgtttg ttttgggaat aatccgataa
     4441 ggcgcaaatc atcagatacc gccccgaatg ctccagataa aaaattcgtt tttccaaagg
     4501 aaaccggtta ttccgttgcc cgttttccat ttttcatcct ttttaacgta tcggcttccg
     4561 cgtccttcaa attgagttac ctgccttccg ccaagcaaac gcccgcgccc ctttcatcat
     4621 ttgaatatcc gtttgaccat atttaccaga taactatctt ctttatataa atcaatcagc
     4681 aaaggcacga tacccggctc ataaaataat tctgtcgtat cagatgtttc ttttaacgcg
     4741 gaaattatct ccaacagttc atacccgtat cggggataag caaacaaata ggggactgct
     4801 tcccgcaaac atgaaaatgc ttcgtaactt tgcatttccc atatatgttc atctttaaaa
     4861 tcactcaaat accattcatc caattcactt tccggatcgg atatcaatcg gataaattca
     4921 tttagttcgt ccttaaacat atcaaatcct taaatataaa atcttttgat tatccgccgt
     4981 atccttattt tccacatcat gaatttcaac gccgttcata ttttgcgttt tctcaaaccg
     5041 tccaccacgg cataaaatta ttttttgtat caattgctta tatatttaac aacttaccgc
     5101 cggacttgct ttggctgcgt atcaagattg tccggttcgc ggcgggcgtt tgctttgttt
     5161 caaacagggc ggcgcgtaag ggattttcaa ggggcggcag gcggaaaaag gaaatgaaaa
     5221 tgccgtctga acggcaaaac gtgcgttcag acggcatttt gggcggatcg cgtcatcgcg
     5281 ccaaagcctt caaacattct ttgaccaatg ccggaccttt gtatatcaat ccgctgtaca
     5341 cctggacggc agttgcgccc aagcggattt tatctgccgc gtccctgcct tccataatgc
     5401 cgcctacgcc gataatcggc agcttgccgt ctatgtggtc tgccaacagc ttcaacaccc
     5461 gattactttt ttcatgaacg ggccgcccgc tcaaaccgcc ctgctcgcct gcgagcggat
     5521 ggctgccgag acttgatttg tcgatggtgg tattggtagc gatgatgccg tccatttcga
     5581 cggatttgac aacgtgggcg atgtcttcga tttgtgcttc atccaaatcg ggggcgattt
     5641 tgacggcgag cggaacgtat ttcccgtata cagaggcaag ctgtgcctgt ttgtttttca
     5701 aagcctcaag caatgcgctc aactcgtcgc caccttgcag cgcgcggagg tttttggtgt
     5761 tgggcgagga aatgttgacg gtaatgtaac ttgcgtgtgc gtaggctttt tcaaggcaga
     5821 ttaaataatc atcggcagcg ttttcgatgg gtgtaaccgc gtttttaccg atgttgatgc
     5881 ccaatacgcc actgaattta cttttttcga tgttgcgtat catggtgtcg ataccgtggt
     5941 tgttgaaacc catgcggttg atgatgcctt ggtgttcggg aacgcggaag aggcgcggct
     6001 gcgggttgcc gggctgcggt ttgggcgtta ccgtgccgat ttcgatgaaa ccaaagccga
     6061 gcgcgcccaa tgcgtcgatg tattcgccgt ttttgtcgag tccagcggca agtccgacag
     6121 ggttgggcaa atccataccc atcaatttta caggtttggt acggttgccg gttacgggaa
     6181 tcagacccaa tttataaacc gtgtagagcg cgtcgagcgt gaagtggtgg gctttctcgg
     6241 catcgagtgc aaacaggatg cggcgggcaa gtggatacat gacagggctt tctgcttcag
     6301 atggaaatgc cgtcatttta accgaaatcc cgtcaaacat acggcacaca ggtcaaactt
     6361 gaacgacaaa acccgccgca cccaaaaatg tacaaatcgg caaatattga tataatagtc
     6421 attagataga aaactctatt aaaagtcggt acaataccac cgatttattt cccttaaaca
     6481 acctgccgca aggcataaag gaacgactga tatgtcaaac atcgaacaac aagttaaaaa
     6541 aattgttgct gaacaactgg gcgtaaacga agccgacgtg aaaaacgaat cttctttcca
     6601 agacgacttg ggcgcggatt ctttggacac cgtggagctg gttatggctt tggaagaagc
     6661 attcggctgc gaaatccccg acgaagatgc cgaaaaaatc accaccgtcc aactggctat
     6721 cgactacatc aatgcccaca acggctaacc ggtcgtcgcc cgacacaaca gcctctgctg
     6781 caccgcgcaa tagaggcttt tcccttatat ggcaaactgt ttgaacccct gccgcaaatt
     6841 acagacggca tctgcacgac agccgcatat gcccccattc cgacaaccaa cagcgaggtt
     6901 atcatgagtc agagaagagt agtcattaca ggcttaggtc aggtttcccc tgtcggcaac
     6961 actgtcgcag aggcttggga caccctgctc accggcaaaa gcggcatcgg cgcgattacc
     7021 cgctttgacg catccgacat caacagccgt gtcgccggcg aggtgcgcgg tttcgacatc
     7081 ggacaataca tcagcgcgaa agaagcgcgc cggatggacg tattcatcca ctacggcatt
     7141 gccgccgcat tgcaggcaat cgccgattcg ggtttggacg atgtggaaaa cctcgacaaa
     7201 gaccgcatcg gcgtgaacat cggttccggc atcggcggac tgcccggcat cgaggtcacc
     7261 ggcaaagccg taatcgaagg cggcgcgcgc aaaatcaacc ctttctttat ccccggttct
     7321 ctgattaatc tgatttccgg acacgttacc atcctcaaag gctaccgcgg cccgagctac
     7381 gggatggttt ccgcctgcac caccggcgcg cacgccatcg gcgattccct ccgtatgatt
     7441 aaatacggcg acgcggacat aatggttgcc ggcggcgcgg aaggcgcaat cagcactttg
     7501 ggcgtgggcg gttttgccgc gatgaaagcc ctctccaccc gcaacgacga ccccgccacc
     7561 gcttcccgtc cgtgggacaa aggccgcgac ggcttcgtta tcggcgaagg cgcgggcata
     7621 ttggtgttgg aagaattgga acacgccaaa aaacgcggcg cgaaaatcta cgccgaaatc
     7681 gtcggcttcg gcatgagttc cgatgcttac catatcaccg cgccgaacga agaaggcccc
     7741 gcccttgccg ttacccgcgc gctgaaagat gccggcatca atcccgaaga cgtggattac
     7801 gtcaacgcgc acggcacgtc cacccccttg ggcgatgcca acgaaaccaa agccctcaaa
     7861 cgcgcgttcg gcgaacacgc ctacaaaacc gtcgtcagct cgaccaaatc catgaccggc
     7921 cacctgctcg gcgcggcggg cggcgtggag gccgtgtaca gcattttggc gatacacgac
     7981 ggcaaaatcc cgccgaccat caacattttt gaacaagacg ttgaagccgg ctgcgatttg
     8041 gactactgcg ccaacgaagc gcgcgacgcg gaaatcgacg ttgccatttc caactccttc
     8101 ggcttcggcg gcaccaacgg cacgctggtc ttcaaacgct tcaaaggctg attccgcaaa
     8161 gccgccgccg acatcgaaat gccgtctgaa accgtttcag acggcatttt tatag
//
LOCUS       DAWGJV010000090         8119 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWGJV010000090 DAWGJV010000000
VERSION     DAWGJV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.090"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..2280)
                     /gene="clpA"
                     /locus_tag="WHQ94_001418"
     CDS             complement(<1..2280)
                     /gene="clpA"
                     /locus_tag="WHQ94_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ2128680.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDEDDGNYSS
                     DGMDDDNENRANPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNN
                     PLLVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARV
                     KSVLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYD
                     EYRTIFDKDHALSRRFQKIDVVEPTVSETVRILRGLKPMFEAFHQVRYTQGALEAAAE
                     LSARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTV
                     SHDDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGV
                     GKTEVAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQP
                     HCVLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRP
                     GLGFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVIAKVVDKFLLQLEHQL
                     LDKKVEAEFTPALRKYLAEKGFDPQMGARPMNRLIQEKIRKPLADELLFGKLAEGGFV
                     RIDWDAAKEEAVLKFKKSKV"
     gene            complement(2282..2584)
                     /gene="clpS"
                     /locus_tag="WHQ94_001419"
     CDS             complement(2282..2584)
                     /gene="clpS"
                     /locus_tag="WHQ94_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ2128681.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            2827..3030
                     /locus_tag="WHQ94_001420"
     CDS             2827..3030
                     /locus_tag="WHQ94_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ2128682.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(3327..4658)
                     /gene="pmbA"
                     /locus_tag="WHQ94_001421"
     CDS             complement(3327..4658)
                     /gene="pmbA"
                     /locus_tag="WHQ94_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ2128683.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            4807..5319
                     /locus_tag="WHQ94_001422"
     CDS             4807..5319
                     /locus_tag="WHQ94_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ2128684.1"
                     /translation="MKMFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLD
                     EDLYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            5366..6085
                     /locus_tag="WHQ94_001423"
     CDS             5366..6085
                     /locus_tag="WHQ94_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128685.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPPFLILDA
                     AGAVLALPVAALIAATNSSDK"
     gene            6154..7854
                     /gene="recJ"
                     /locus_tag="WHQ94_001424"
     CDS             6154..7854
                     /gene="recJ"
                     /locus_tag="WHQ94_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ2128686.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDVEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTSFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
BASE COUNT         1736 a         2084 c         2357 g         1942 t
ORIGIN      
        1 gaccttgctt ttcttaaatt tcaacacggc ttcttctttt gccgcatccc agtctatccg
       61 cacaaagccg ccttcggcga gtttgccgaa caggagttcg tcggcgagtg gtttgcggat
      121 tttttcctga atcaggcggt tcattgggcg cgcgcccatt tgcgggtcga aacctttttc
      181 cgccagatat ttgcgcaatg cgggcgtgaa ttcggcttcg acttttttgt cgagaagctg
      241 gtgttcgagc tggagcagga atttgtccac gactttggcg atgacgggtt cggacagggg
      301 tgcaaacggg ataatcgcat ccaagcggtt gcggaattcg ggcgtgaaga gcttgttgat
      361 ggcctgcatt tcgtcgccgc gttcgcgctt agcggtaaag cctaggccgg ggcggctgag
      421 gctttccgcg cctgcgttgg tggtcataat caggatgacg ttgcggaaat cggcactctt
      481 gccgttgttg tcggtcagtt tgcctgcgtc catgacttgc aggaggacgt tgaaaatgtc
      541 ggggtgggct ttttcgattt cgtccaagag caatacgcag tgcggctgtt tgttgacggc
      601 ttcggttaaa aggccgcctt gttcaaagcc gacatagccc ggaggcgcgc cgatgaggcg
      661 cgatacggca tggcgttcca tgtattcgga catatcaaag cgttgcagcg gtacgcccat
      721 cgagtaggca agttgtttgg cgacttcggt tttaccgacg ccggtcggac cggagaagag
      781 gaaactgcct atcggtttgt cgggcagggc aaggccggaa cgcgacattt tgacggcagc
      841 aaccagcgcg tcgatggcgt tttcttgacc gtaaaccatg tttttcaaat cgcggccgag
      901 gaattgcagc acttgtttgt cgtcgtgcga cacggttttt tcgggaatcc gcgcaacttt
      961 ggcgatgacg gtttcaattt gtgctttgcc gatgactttt ttctgtttgg atttgggtaa
     1021 aatccgttgc gccgcgcctg cttcgtccat tacgtcgatg gctttgtcgg gcaggaaacg
     1081 ctcgttgatg tagcgtgcgg agagttcggc ggcggcttcg agtgcgcctt gagtgtagcg
     1141 gacttggtgg aaggcttcaa acatcggttt caagccgcgc aggattcgta cggtttcgga
     1201 aacggtgggt tcgaccacgt cgattttttg gaagcggcgg cttaaggcgt ggtctttgtc
     1261 gaaaatggtg cggtattcgt cgtaagtggt cgcgccgatg cagcgcagcg aaccttttgc
     1321 cagcgcgggt ttgagcaggt tggacgcgtc catggtgccg ccgctggtgc tgcccgcgcc
     1381 gatgatggtg tggatttcgt cgataaacaa aatggcgtgc gggatttttt cgagctgttt
     1441 caagacggat ttgacccgcg cttcaaagtc gccgcggtat ttcgtgcccg ccaacagcga
     1501 gcccatatcc agcgcgtaca cttcggcatc tttgagcgcg tctggaatgc cgccgttgac
     1561 gatttgatgt gccagccctt ccgccagcgc ggttttgccc acgcccgctt cgccgaccaa
     1621 aagcggattg tttttgcggc ggcggcacag gatttgcacc agccgttcca tttcgtgttt
     1681 gcgaccaatc aaagggtcga tacggccggc tttgacttcg gcgttgaggt tgacggtgta
     1741 cgccgataaa gggtttttgc ccgggttggc gcggttctca ttatcgtcgt ccatgccgtc
     1801 tgaagaatag ttgccatcgt cttcatcttc atcttcatct tcatcgggag agccgtgggc
     1861 aatacagcgc aaaacttcaa aacgcgtaac cgattgcagc ttgaggaaat agacggtgtg
     1921 gctgtcggtt tcgctcatca gcgcgaccaa aacgtccaac ggttcgactg cggcttttcc
     1981 agcagactgg gtatgcacca tcgcccgttg catcacgcgt tggaagccga gcgtgggccg
     2041 ggtttcgacc gtgtctaaaa ggtgttcggg aatcaggggg gtgttttcgg caacgctggc
     2101 ggcgagctgt tcggacacca ctttcaaatc cgcgccgcat agttttaaga cgttcggcac
     2161 ggaggcatct tcttcgatga gtaccaaaag cagatgctcg aggctgataa attcataatg
     2221 agccttacgc gcctcgcggt aaagctgctg caaaatctgt tccaattcgg gtgcaagcat
     2281 attaaatctc ctcgacaata cattgcagcg gatgcccttc ggcttttgcc cgctgcatga
     2341 cttgttgttg tttggtttgg gcaatatcgc gcgtgtaagt gccgcacagg cctttgcctt
     2401 cgtgatgaac caagagcatc accgctaccg cctgttcttg tccgagcata aagatttcgg
     2461 tcaggatttc gacgacaaat tccatcgtgg tgtaatcgtc gttcaatagg aaaacgccgt
     2521 aacgtttcgg cggcagggtg ttcagacggt gcaagagcgt gtcggattgg tgttgcgcgg
     2581 tcatagtgtg tcccatttga aagccgcgtt cagacggcat ttttgttgta ttttcggtac
     2641 ttttgcctat tttcccactt ttttgaaaac atagcttgac gttttgtctt aacaaatgta
     2701 aaaagacggt taagcaggat tgggcatccg ccgagtatat caagctggaa ggaacgccag
     2761 acggctcggt ttgccgtatg ccttgttttg ctgattttgt taatttttga gtataggaag
     2821 tttctaatgg caaccggtat cgtaaaatgg tttaacgacg ctaaaggttt tggtttcatc
     2881 acgcctgatg aaggcggcga agatttgttc gctcacttct cagcaatcaa tatggaaggt
     2941 ttcaaaaccc tgaaagaagg ccaacgtgtc tctttcgacg taaccaccgg ccctaaaggc
     3001 aaacaggccg ccaacattca ggctgcttaa ttcctgatgt acggtcaaat gtatatttga
     3061 aaacggcggg acaggcaatg tcccgccgtt tttgtctgcc gttttgccgg cggcggaaaa
     3121 accccaatcc ccgcacgcct tatcctgaac ttgtgtgtac cctgttgtgg acaagtggct
     3181 tagtattttg acggataagg gaaaatcagt gctgatgaaa aaatgtgcaa tgttgtcggc
     3241 aaaaggcggt gcggcataaa acggcaaacg ggcaggcacg gggcaaaacg tgctgccttc
     3301 gtcttcagac ggcatcggca gggcgttcag cttccggcaa ccgtcatccc cgcaatcaga
     3361 atcgagccga ttttgttgga cgaacgccgc aaagcgtcat ccgccacgcc gacaatgtcg
     3421 cggtacatat cctgcaagcg tccggctacg gtaatctcgt ggacggggta ggcaatcacg
     3481 ccgttttcca cccaaaaacc cgccgcgccg cgcgagtagt caccggtaat ggtgttcgcg
     3541 ccctgtccca tcagttcggt taccaacaat cccgtcccca tttctttcag caggtcggat
     3601 tgcgtttcgt gcgtatggtt caaatacagg ttgtgcgcgc cgccggcgtt gcccgtggtc
     3661 tgcataccga gtttgcgcgc gctgtaactg ctgaggaaat agccttcgac aatgccgttt
     3721 tgaatcacga agcgcggcgc ggtggcaacg ccttccgcat caaaatagct gctgcggaaa
     3781 gagcggggga tgtgcggttc ttcgcgcagg ttgaggaaat cgggcaggac ttttttgccg
     3841 atgctgtcga tcaggaaact gctttggcgg tagagcgcgc cgccggagag tgcgccgacg
     3901 aggtgtccga taagaccgcc cgaaacggtg gtatcgaaga ggacggggta gctgcctgtc
     3961 gggatgctgc ggctgccgag tcggcgcaaa gtgcggcggg cggcggtttg accgatggtt
     4021 tcggggctgt ccatatccgg atggcggcag gcggaatcgt accagtagtc gcgctgcatg
     4081 ccgttttcgt cggcggcaac gacgctgcag gaaatgctgt ggtgcgtgcc ttgccggtgt
     4141 gcggcaaaac cgtgggtgtt gccgtaaacg tattggtaat ggccggtttg caccgccgcg
     4201 ccttcggagt tttcgatgcg ctcatcctcg ttcagggcgg cttgttcgca ttgttttgcc
     4261 aagccgacgg cggcttccgt atccaaatcc cattcgtggt aaaggtcggg gtcgccgatg
     4321 tgttttgcca tcagacaggc atcggcaagt ccggcgcaac cgtcttcggc ggtgtggcgg
     4381 gcgatgtcgg cggcggcgcg gacggtgtcg cgcagggctt gttcggagaa atcggcggtg
     4441 ccagcgcggc ctttgcgttt gccgacgtaa acggtaatgt ccagcgactt gtcctgctgg
     4501 aactcgattt gttcgatttc gcccagccgc acgctgacgc tttgtccgat ggattcgctg
     4561 aaatcggctt cggcggcggt cgcgcccgct gcttttgcca agtcgagcgt gcggcggcag
     4621 aggtcgagaa gttcggaagc ggtgtggttg aacagcatag aaatctttct tggtggagcg
     4681 ttgcggcatt ttaaccgttt cgggcggcag gggcaaaagc gtgccgtttg cagggcggac
     4741 ggtgcaaaat gccgtctgaa cgcggaatca ttctgttaaa atgcgcccgt cggataaaat
     4801 ttgagaatga agatgtttga acaagaagac gaatgggtca gcaaaaccca aatgaaaaag
     4861 cagatgaacg gtttgcagga tttgggtatg gagctgacca agctctcaag cgacacgttg
     4921 aaaaaaatcg gcttggacga agatttgtac gaagccgtcg ttacctataa aaaaatcaca
     4981 tccaacggcg cgctcaagcg gcaggcgcaa tttatcggca gactgatgcg cgataccgat
     5041 cccgcgccca tcgaggcatt tcttgccaag ctgcgcggcg acgatgcggt gcacaatgcc
     5101 tttttgcaac gcgtggaaca ggcgcgcgta cggctgttgg cagacgacgg cgcgttgacg
     5161 cagtttatgt cggattttcc gcatgcggac gcgggcaagc tgaggacgct cgtccgcaac
     5221 accaaaaaag agcaggagca aaacaaaccg ccgaaaaact tccgcgcgct gtttcaagaa
     5281 ttgaaaaccg tgatggaaaa cggggacgcg gaaatttagg catattttca gacgacatcc
     5341 gccgttattt agattggagg ataaaatgtt gttccgtaaa acgaccgccg ccgttttggc
     5401 ggcagccttg atgttgaatg gctgtacggc aatgatgtgg ggcatgaacg acccgttcag
     5461 ccaaacgacc gcctataaac acgttgacaa ggatcaaatc cgcgccttcg gtgtggttgc
     5521 caaagacaat gcccaattgg aaaagggcag tctggtgatg atgggcggga aatactggtt
     5581 cgtcgtcaat cccgaagatt cggcgaagct gacgggcatt ttgaaggcag ggctggacaa
     5641 acccttccaa atagttgagg ataccccgag ctatgctcgc caccaagccc tgccggtcaa
     5701 actcgaatcg cctggcagcc agaatttcag taccgaaggc ctttgcctgc gctacgatac
     5761 cgacaagcct gccgacatcg ccaagctgaa acagctcggg tttgaagcgg tcaaactcga
     5821 caatcgaacc atttacacgc gctgcgtatc cgccaaaggc aaatactacg ccacaccgca
     5881 aaaactgaac gccgattacc attttgaaca gagtgtgcct gccgatattt attacacggt
     5941 tactgaaaaa cataccgaca aatccaagct gtttgcaaat atcttatata cgccccctcc
     6001 ctttttgata ctggatgcgg cgggcgcggt actggccttg cctgtcgcgg cgttgattgc
     6061 agccacgaat tcctcagaca aatgaacggc aatgccgtct gaaaagcctt cagacggcat
     6121 ttcaaacaca tccgcactgt aaagttccac gctatgtccg tcaaaatcca aacccgatcc
     6181 gtcaataccg acgttttcaa tcatttgctc accgccggcg ccgatccttt aatcgcccgg
     6241 ctttgtgcct cgcgcggtgt gcaaagtcct gccgaattgg acgacaaact cgcttccctc
     6301 ctgccttatc aaacgctgac gaattgcgaa gccgccgccc gccgtttggc ggatgcggtt
     6361 gagcgtcagg aaaaaatctt gattgtcgcc gactacgatg ccgacggcgc gaccgcgtgt
     6421 gccgtcggtt tggacggttt ggcggcgatg ggggcaaaag tagatttcct cgtgcccaac
     6481 cgctttgaac acggctacgg tttaacgccc gagctggccg aaatcgccgc cgcgcaaggc
     6541 gtggatttgc tgattacggt cgataacggc attgccagca tcgcaggcgt ggcgcgtgcg
     6601 caggaattgg gtttggatgt gattgtaacc gaccaccatc tgcctgccga gaccgtgcca
     6661 gactgcatca tcgtcaatcc gaaccaaaaa ggctgcggtt tccaaagcaa aagcttggcg
     6721 ggcgtgggcg tgatttttta tgtactgatg gctttgcgtg ccgaactgcg ccgccgcaat
     6781 tatttttcag acggcctaaa agagccgaat ctgggcgacc ttttggattt ggtcgcactc
     6841 ggtaccgttg ccgacgtcgt ctccctcgat cacaacaacc gtatcctcgt gtcgcaaggt
     6901 ttgaaacgga tgcgttcggg caaaatgcgc cccggtatcc gcgccttgtt tgaagtggcg
     6961 cggcgcgact ggcgcaaagc gcagccgttt gatatgggct ttgcgttggg gccgcgcatc
     7021 aatgccgccg gacggttgga cgatatgtcg gtcggcattg cctgtctgtt ggcgcgagat
     7081 gatgtggaag cgcaggaact ggcggctcag ttaaacaacc tcaatatcga gcgtcgcgaa
     7141 atcgagcagt ctatgctgca agacgcgctg aatgcttttc ctgaaaccct gccttcaggt
     7201 cagacgactt tggtggcgta tcgcgacgac ttccatcaag gtgtggtcgg cattgtcgcc
     7261 agccgcctca aagaccgttt ttatcgtccg accatcgtgt ttgcgcctgc cgacaacggc
     7321 gaagtacgcg gttcgggacg ttccattccc aatttgcacc tgcgcgatgc tttggacttg
     7381 gtgtccaaac gccatcccga tttgattttg aaattcggcg gacacgcgat ggcggcgggt
     7441 ttgagcatac ttgaacacaa cattcccgcg tttcagacgt cctttgaaga agccgtgcgc
     7501 gaaatggtgt gcgaagacga tttgtcgcaa accttcatca ccgacggcag cctgcccgcc
     7561 tgcgacatca cgttggaaca ggcgcaaaac cttgcccgtc acgtttgggg gcagggcttc
     7621 gcgccgccga gctttaccga cgagttccac gtcgtccgcc agcaaccttt gggcgcggag
     7681 ggcaaacaca aaaaagtctg gctgcaaaaa gacggctgcg aatttgaagc catgttttgg
     7741 cgttgcagcg aagacatccc cgaatacatc cgcacggttt accgccccgt tgccaacgaa
     7801 tggcgcaaca atctcgaatt gcagctgtat atcgattact gggaagcggt gtagaggcgg
     7861 cggaacgccg tttgaatgtg atttctgttc cttcatttgc ctgtttgtac gacgggaatg
     7921 ttcccaatcg gaaaagaaag taaagggcgt taatttttgt taatcatccc ttcttaggga
     7981 tgcaatatat aaggtgcatc aaatttcaga ctccggcgga tttgcatttg aagtgcaact
     8041 ttccctaaca gaaaaaggcc agtatgcggt agcatacgac ctttcctgca agaaagattg
     8101 ccatgagcta cacgcaact
//
LOCUS       DAWGJV010000091         8022 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWGJV010000091 DAWGJV010000000
VERSION     DAWGJV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.091"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(68..814)
                     /locus_tag="WHQ94_001425"
     CDS             complement(68..814)
                     /locus_tag="WHQ94_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ2128687.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WHQ94_001426"
     CDS             complement(993..1664)
                     /locus_tag="WHQ94_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ2128688.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WHQ94_001427"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WHQ94_001427"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ2128689.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WHQ94_001428"
     CDS             complement(2481..2963)
                     /locus_tag="WHQ94_001428"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ2128690.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WHQ94_001429"
     CDS             complement(2995..3684)
                     /locus_tag="WHQ94_001429"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ2128691.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGAEKLLEIGLASAD
                     DNILVHDAVRCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WHQ94_001430"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WHQ94_001430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ2128692.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHE
                     KYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(4573..5883)
                     /locus_tag="WHQ94_001431"
     CDS             complement(4573..5883)
                     /locus_tag="WHQ94_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2128693.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5873..6058)
                     /gene="ccoS"
                     /locus_tag="WHQ94_001432"
     CDS             complement(5873..6058)
                     /gene="ccoS"
                     /locus_tag="WHQ94_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ2128694.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6058..6357)
                     /locus_tag="WHQ94_001433"
     CDS             complement(6058..6357)
                     /locus_tag="WHQ94_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128695.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6708..7904
                     /locus_tag="WHQ94_001434"
     CDS             6708..7904
                     /locus_tag="WHQ94_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ2128696.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITSADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1892 a         2415 c         1950 g         1765 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcatagt cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaaagtgg
     1921 tgtagccgat acgcaattca ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcgtc cgcaatgcag acgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaatg tcgtactggg
     2221 caagcagttc gctggatttt ttggaggttg aaaccgcgcc tttgattttt ttgccgcttt
     2281 tgcccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcacggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt tttccgcacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgaa acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gccccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tcgagatatt tttcgtgcgc cgccagttct
     3781 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3841 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atcgctaaat aaacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac agggcatcca ggctggcttt ctgcccaggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcgacagtc
     4081 ggcagcccca tccggcggaa ctccatattg aggaagccca catcgaattt ggcattgtgg
     4141 ataatcaact ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaaaggc
     4201 ggtgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttca
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc catcggcata caggccggtg
     4381 gtttcggtat cgaggatgat ttggcgttga atcatgtctg cttctttctt caaattttcc
     4441 atctgctgac tattttttag gcagcgtatt tttctgtttt catttcaatg cacaaaccca
     4501 cttattcaca gtgtgttcac aacattgggc aggcggattg tgtattttgg ggacaatttt
     4561 ttcagacggc attcaaggtt ttttcctgat tgccgccgcg cctaaaaacc gcctttcgcg
     4621 cttaatcaaa aataccgaca acggaatatt gcccaaagcg acaatcagat acaacaagga
     4681 aatgctgtca aacaaaaaca gcaacaccgc gctcaaaacg gcggcggaaa ccataaaaat
     4741 accgttaacg atattgttgg cggcaacggc gcgggcgcgg aaagtctcgc tactggcggt
     4801 ttgcagccaa gtatagagcg gaacggagaa aaatccgccg aaaaagccga tcagcgtcat
     4861 caccgccatc acgggatatg cccatccttg cgataaaaac caaaaaatgc cgttcagccc
     4921 ttcaaaacgg tgtccgtgcg tcagccacac caaaaccagg ccgcaaaccg tcaaacccaa
     4981 cgcaccaacc gttacccaag ccaacatcag ccgttccctg ctgaacttgg cacacagtac
     5041 cgaaccggcg gcaataccga tggaaaacag ggcaagcatc aggttgaaaa cattgtcgtt
     5101 gccgcccaga tggatttggg taaaggtcgg cagttgcgtg gtataaaccg cgccgacaaa
     5161 ccaaaaccac gaaataccga taatggcggt aaaaacgggc ttgtgccgca ccgtttcact
     5221 cagcagggat tttgtgccac ggacaatatt ccactcaatt tgtgtatcgg cagccttggc
     5281 gggtacggac ggcataaaca ggctgccgac cgtgcctccg acggcgacca gcaaaaccag
     5341 tatcccgaca atataaggcg gtacacctgc caccgccgtt cccaaaatct gaccgaacag
     5401 gatggcgaca aacgtacccg attcaatcag gctgttgccc atcatcaact ctttgtcgtc
     5461 gagataatcg ggcaggatgg cgtatttcag cggcccgaac agcgtcgatt gcgcgcccat
     5521 gcaaaacaga cacgccaaaa gcagcggggc agaccggata taaaacccgt atgccgccac
     5581 cgccataatg atcatttcca gcaccttgac ccaacgcgcc aaaacggcct tgtcgaattt
     5641 gttacccaac tgccccgaca gcgaggaaaa caggaaatac ggcaaaataa acagcaacgc
     5701 gcccaagttc aacatctgtc cggcaggcag gaagccgttt tgccccaaac cgtaaaaccc
     5761 aatcatcaca aacagcgcgg ttttgaacac attgtcgttg aacgcgccga gaaactgcgt
     5821 agcgaaaaga ggtgcgaaac ggcggctttt aaccagtccc aaaccgcctt ttttagcgta
     5881 catcgttttc cctctctttt tcaatcagtt tacttgtcga atcatcatcc atcaggatgc
     5941 ggtgcgccgg cccttccaag tcgtcaaact gcccgttttt gcccgaccac caaaaaaacc
     6001 agccgatgac aaacgccaaa ataatgctga tgggcaccaa tataaacatg ctttccatca
     6061 catattccct gtcaaatcgt tcaaaacaaa agtctgcccc gacacggtca gatattcgtt
     6121 acgcaaagtt ccgacaggtg cttcgtcaaa aaacagctcg atacggtctt tgaccacgcg
     6181 ccaatattgg gggatttccg tctgaccgaa cggcgacagg acatgatttt ccattccgcc
     6241 ttcaagtttg acggcaaaac gcccgctttg cggccgtgct tccgattcgt cgtcggcaag
     6301 caggatgaaa aagcctatat gccgtcccga ttggtcatga atactgaaat aatgcataaa
     6361 tttcccaccc gccttttttc agacgacacc aactaaaaac agggcgaatg taccagtttg
     6421 gacgggaaga atgcaaagaa attctccctc ccccagccga aaacaccggc aaaccgcata
     6481 tccccctttt ttccgtcaaa atacccgact tccgccattt tcacgcaaac gcccgattaa
     6541 gccaagcaat tgcaaagatt ttttgttaga ataacctgct tcttttatca accgtttcag
     6601 acggccccac tactttcccg cccaggaagg caaaacggat tcggcacgaa tccggttagt
     6661 atccgtgtcc gattccaatg ccgtctgaaa ctttccggag taagaaaatg tcccaaaaat
     6721 tgatcttggt tttgaactgc ggcagctcgt ccctcaaagg cgcggtcctg gataacggca
     6781 gcggcgaagt cctgctcagc tgccttgccg aaaaactcaa cctgcccgat gcctacatca
     6841 cattcaaagt aaacggcgaa aaacacaaag tcgatctgtc cgcacatccc gaccacaccg
     6901 gcgcggtcga agccctgatg gaagaactca aagcccacgg cctcgacagc cgcatcggcg
     6961 ccatcggcca ccgcgtcgtc agcggcggcg aactgtacag cgaatccatc ctcgttgacg
     7021 acgaagtcat tgccggcatc gaaaaatgca tcccgctcgc ccccctgcac aaccccgccc
     7081 acctcttggg cctgcgtgcc gcgcaaagca ttttcaaagg cctgcccaac gtcgtcgtat
     7141 tcgatacctc cttccaccaa accatgcccg aagtcgccta caaatacgcc gttccgcagg
     7201 agttgtatga aaaatacggc ctgcgccgtt acggcgcgca cggtaccagc taccgcttcg
     7261 tcgccgacga aaccgcgcgc ttcctcggca aagacaaaaa agacctgcgt atggtcattg
     7321 cccacttggg caacggcgcg tccatcaccg ccgtcgccaa cggcgaatcg cgcgacacca
     7381 gtatgggcct gaccccgctg gaagggctgg taatgggtac gcgcagcggc gacatcgatc
     7441 cttccgtatt cggcttcctc gccgaaaacg ccaatatgac catcgcccaa atcactgaaa
     7501 tgctgaataa aaaatccggt ctgctcggca tttccggcct gtccaacgac tgccgcacca
     7561 ttgaagaaga agccgccaag gggcataaag gcgcgaaatt ggccttggat atgtttatct
     7621 accgccttgc caaatacatc ggcagtatgg cggttgccgc aggcggtttg gacgcactgg
     7681 tctttaccgg cggcatcggc gaaaactccg acatcatccg cgaacgcgtg atcggctact
     7741 tgggcttcct cggtctgaac atcgaccaag aagccaacct gaaagcccgc ttcggcaacg
     7801 ccggcgtgat tacctccgcc gacagcaaag ccgttgccgt ggtcattccg accaacgagg
     7861 aattgatgat tgcacacgat accgcccgct tgagcggtct gtaagattca atccccacgc
     7921 gaactgcctc cgaaaacgga ggcagttttt ttatccggct ttccatgctt aaacagcact
     7981 gcctcttttc agacattgac ggttgcagcc gcttacctga ac
//
LOCUS       DAWGJV010000092         7992 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWGJV010000092 DAWGJV010000000
VERSION     DAWGJV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.092"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            108..1277
                     /gene="prpF"
                     /locus_tag="WHQ94_001435"
     CDS             108..1277
                     /gene="prpF"
                     /locus_tag="WHQ94_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ2128697.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1691..2890
                     /locus_tag="WHQ94_001436"
     CDS             1691..2890
                     /locus_tag="WHQ94_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ2128698.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2998..3618
                     /locus_tag="WHQ94_001437"
     CDS             2998..3618
                     /locus_tag="WHQ94_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ2128699.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3745..4188)
                     /locus_tag="WHQ94_001438"
     CDS             complement(3745..4188)
                     /locus_tag="WHQ94_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ2128700.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4362..4730
                     /locus_tag="WHQ94_001439"
     CDS             4362..4730
                     /locus_tag="WHQ94_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2128701.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4775..5929
                     /locus_tag="WHQ94_001440"
     CDS             4775..5929
                     /locus_tag="WHQ94_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ2128702.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5986..6858)
                     /locus_tag="WHQ94_001441"
     CDS             complement(5986..6858)
                     /locus_tag="WHQ94_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ2128703.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIATHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6943..7755
                     /locus_tag="WHQ94_001442"
     CDS             6943..7755
                     /locus_tag="WHQ94_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ2128704.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSSEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSADGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1718 a         2209 c         2321 g         1744 t
ORIGIN      
        1 ggcgaacccc gacagtttgc ggaatcaaac ggctttggtc ggagtggcag cctaatccac
       61 tataaaaatc gtgggcagag cccacgctac ataaggagaa tctagaaatg ccgcaaatta
      121 aaattcccgc cgtttactac cgtggcggta catcaaaagg cgtgtttttc aaacgttccg
      181 acctgcccga ggcggcgcgg gaagcgggaa gcgcacgcga caaaatcctc ttgcgcgtac
      241 tcggcagccc ggatccctac ggcaagcaga tagacggttt gggcaacgcc agttcgtcca
      301 ccagcaaagc cgtgattttg gacaagtccg aacgcgccga tcacgatgtc gattaccttt
      361 tcgggcaagt ttccatcgac aaaccttttg tcgattggag cggcaactgc ggcaacctca
      421 ccgctgccgt gggcgcattc tccatcgaac agggcttggt cgataaaggc aagattcctt
      481 cagacggcat ctgcacggtc aaaatctggc agaaaaacat cggcaaaacc attattgccc
      541 atgtaccgat gcaaaacggc gcagttttgg aaacaggcga ttttgagctc gacggcgtaa
      601 cgttcccggc agccgaagta caaatcgaat ttcttgatcc agccgacggc gaaggcagta
      661 tgttcccaac cggcaatttg gtcgatgaaa ttgatgtgcc gaatataggc cgtttgaaag
      721 ccacgctcat caacgcgggc attccgaccg ttttcttgaa tgccgccgac ttgggctaca
      781 caggcaaaga gttgcaagac gacatcaaca acgatgccgc ggctttggaa aaattcgaga
      841 aaatccgcgc ttacggtgcg ctgaaaatgg gtctgatcag cgacgtatcc gaagctgccg
      901 ctcgcgcgca cacgccgaaa gtcgccttcg tcgcgcccgc cgccgattac accgcctcca
      961 gtggcaaaac cgtgaacgcc gccgacatcg atttgctggt acgcgccctg agcatgggca
     1021 aactgcacca cgcgatgatg ggtaccgcct ctgttgccat tgcgaccgcc gccgccgtac
     1081 ccggtacgct ggtcaacctt gccgcaggcg gcggaacgcg taaagaagtg cgcttcgggc
     1141 atccttccgg cacattgcgc gtcggtgcag ccgccgaatg tcaggacgga caatggacgg
     1201 ccaccaaagc ggtcatgagc cgtagcgcac gcgtgatgat ggaaggttgg gtcagggtgc
     1261 ctgaggattg tttttaaatt gacgtagcat gggtttgccc gcgagccata aaaaggtcgt
     1321 ctgaaaaaca agtaaacatc aaatcactga ccattccttt cccttgccct gtggcggaag
     1381 gcggcaaatc acaaggaaga acacggaaac cccgataaaa gacagcttcc cgtattaccg
     1441 tcattcccgc gcaggcggga atccagacct gtcaatatgg aggattggca ggggaaaaca
     1501 ggtttcgtga gttctacatt ctggattccc gccacagcct gtcctcgcgt aggcggggac
     1561 ggaataacga tagaaaatgc ggcatatgct ttgcccaaag aggccgtctg aaacacactc
     1621 cgcccaacgc ccatcctttt tcagacgacc ccacaccaaa aaacaaccac aaactacaag
     1681 gagaaacatc atgtccgacc aactcattct tgttctgaac tgcggcagtt catcgctcaa
     1741 aggtgccgtt atcgaccgca aaagcggcag cgtcgtccta agctgcctcg gcgaacgcct
     1801 gaccacgccc gaagccgtca ttacgttcag caaagacggc aacaaacgcc aagttcccct
     1861 gagcggccgc aactgccatg ccggcgcggt gggtatgctg ctgaacgaac tggaaaaaca
     1921 cggactgcac gaccgcatca aagccgtcgg ccaccgcatc gcccacggcg gcgaaaaata
     1981 cagcgagtct gttttgatcg accaggccgt aatggacgaa ctcaatgcct gcattccgct
     2041 tgcgccgctg cacaaccccg ccaacatcag cggcatcctt gccgcacagg aacatttccc
     2101 cggtctgccc aatgtcggcg tgatggatac ttcgttccac caaaccatgc cggagcgtgc
     2161 ctacacttat gccgtgccgc gcgagttgcg taaaaaatac gctttccgcc gctacggttt
     2221 ccacggcacc agtatgcgtt acgttgcccc tgaagccgca cgcatcttgg gcaaacctct
     2281 ggaagacatc cgcatgatta ttgcccactt aggcaacggc gcatccatta ccgccatcaa
     2341 aaacggcaaa tccgtcgata ccagtatggg tttcacgccg atcgaaggtt tggtaatggg
     2401 tacacgttgc ggcgacatcg atccgggcgt atacagctat ctgacttccc acgccgggat
     2461 ggatgttgcc caagtggatg aaatgctgaa caaaaaatca ggtttgctcg gtatttccga
     2521 actttccaac gactgccgca ccctcgaaat cgccgccgac gaaggccacg aaggcgcgcg
     2581 cctcgccctc gaagtcatga cctaccgcct cgccaaatac atcgcttcga tggctgtggg
     2641 ctgcggcggc gttgacgcac tcgtgttcac cggcggtatc ggcgaaaact cgcgtaatat
     2701 ccgtgccaaa accgtttcct atcttgattt cttgggtctg cacatcgaca ccaaagccaa
     2761 tatggaaaaa cgctacggca attcgggcat tatcagcccg accgattctt ctccggctgt
     2821 tttggttgtc ccgaccaatg aagaactgat gattgcctgc gacactgccg aacttgccgg
     2881 catcttgtag ccaaaaaagg gacgagtccg caaaaatgcc gtctgaaatt ttcagacggc
     2941 attttgtttg gattttgggc gtacaatacg gaaaacatga cgataaggac acaaactatg
     3001 gcacagtttt tcgccgttca tcccgacaac ccccaagaac gcctcatcaa gcaggcggtc
     3061 gaaatcgtca ataaaggcgg cgtggtcgtt tatccgaccg attcctgtta tgccttgggc
     3121 tgcaaactcg gcgataaggc ggcgatggaa cgcatactct ccatccgcaa aatcgatttg
     3181 aaacaccacc tgaccctgat gtgcgcagat ttgagcgagt tgggcacata cgccaaagtc
     3241 gacaacgtac agtttcgtca gcttaaagcc gccacacccg ggccttatac ttttatttta
     3301 caggcgacga aggatgtgcc ggcgcgcacg ctgcacccga aacgcaaaac catcgggctg
     3361 cgtattcccg ataatgccat tgcacaagcc ctgctggggg aattgggcga gccgctttta
     3421 agctgcaccc tgatgctgcc cgaagacggc gaaccattga ccgatcctta tgaaatccgc
     3481 gagcgtttgg aacacgccgt cgatttggtg attgacggcg gctggtgcgg aaccgagccg
     3541 accaccgtcg tcgatatgac cgacggcacg gaattggtgc gccaaggttg cggcgatacg
     3601 gcggtgttcg gtttgtaggg aaaccgatgc cgtctgaagc atcggctgtt cagacggcat
     3661 tgcgcgcctt gccggcggca gtccgaaatg ccggcgcgta tcgcgctcgg tcggaatatc
     3721 cgtttgaaac ggcattttga tgcattactg caccgcaatc ggaattctcg gttcgtagag
     3781 caggtcgtag gtcggcttgt tgagcaggtc ttggagcgtg aaaccgtcca gatacgtgaa
     3841 aaacgacttc atcgcgccgc cgagtatgcc cgtcagccgg caggacggtg taatcaggca
     3901 ttcgttgttc tcgcccatgc actcgaccag ctgcatcggt tcgaggtggc ggacaaccga
     3961 gccgatgttg atgcggtcgg gcggtgcggc aagccgcaga ccgccgcctt ttccgcgcac
     4021 actgtggagg aagccgcctt tgaccagcgc ggtaacgacc ttcatcagat ggcttttgga
     4081 aatgccgtag gttacggcga tggtactgat gttgaccagc gcatcgtcgt tgatggcagt
     4141 gtagataagg acgcgcagcc cgtagtccgt atgttgtgtc aaatacatga ttttctcggt
     4201 atggattgtt attcttatcg gtacggttta aggttcacgg acaatacctt aatggttgaa
     4261 accctgtccg tcggggcggt agaatgcagc ctgtctgcgg cggtatgccg tctgaaacat
     4321 ccgcgctacc gtttgataat ttgttattgt aactcaaaat catgaaaccg ttgaaacgac
     4381 atcccgccct tatcgggctt tcgcgtgacc accaccattc gctttccctg tgcgtgcgtc
     4441 tgttgcggac gccggaagaa aggcatcggg acgaactcga accgcatttt gccgaattgg
     4501 aaacccattt tcgcgaagaa gaagccaagt ttgccccaat ttggcagaat gtcgcccccg
     4561 aattgaaaca acgtttcgag aaagaccacg cccgactgcg gcagatgatg gcaagccccg
     4621 aatacggtaa cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac cacgcgcgct
     4681 ttgaagaacg cgagctgttt cccgccgccg aaccgttttt gccggcatga ttccgttttg
     4741 cggcaaatat attaatgata aacaaggaac acacatgaaa tttaccaagc accccgtctg
     4801 ggcaatggcg ttccgcccat tttattcgct ggcggctctg tacggcgcat tgtccgtatt
     4861 gctgtggggt ttcggctaca cgggaacgca tgagctgtcc ggtttctatt ggcacgcgca
     4921 tgagatgatt tggggttatg ccggactggt cgtcgtcgcc ttcctgctga ccgccgtcgc
     4981 cacttggacg gggcagccgc ccacgcgggg cggcgttctg gtcggcttga ctatcttttg
     5041 gctggctgcg cggattgccg cctttatccc gggttggggt gcgtcggcaa gcggcatact
     5101 cggtacgctg tttttctggt acggcgcggt gtgcatggct ttgcccgtta tccgttcaca
     5161 gaatcaacgc aactatgttg ccgtgttcgc gctgttcgtc ttgggcggca cgcatgcggc
     5221 gttccacgtc cagctgcaca acggcaacct aggcggactc ttgagcggat tacagtcggg
     5281 cttggtgatg gtgtcgggtt ttatcggtct gattggtacg cggattattt cgttttttac
     5341 gtccaaacgg ttgaatgtgc cgcagattcc cagtccgaaa tgggtggcgc aggcttcgct
     5401 gtggctgccc atgctgaccg ccatgctgat ggcgcacggc gtgatgcctt ggctgtcggc
     5461 ggctttcgcg tttgcggcag gtgtgatttt taccgtgcag gtgtaccgct ggtggtataa
     5521 gcctgtgttg aaagagccga tgctgtggat tctgtttgcc ggctatctgt ttaccggatt
     5581 ggggctgatt gcggtcggcg cgtcttattt caaacccgct ttcctcaatc tgggtgtgca
     5641 tctgatcggg gtcggcggta tcggcgtgct gactttgggc atgatggcgc gtaccgcgct
     5701 tggtcatacg ggcaatccga tttatccgcc gcccaaagcc gttcccgttg cgttttggct
     5761 gatgatggcg gcaaccgccg tccgtatggt tgccgtattt tcttccggca ctgcctacac
     5821 gcacagcata cgcacctctt cggttttgtt tgcactcgcg cttttggtgt atgcgtggaa
     5881 gtatattcct tggctgattc gtccgcgttc ggacggcagg cccggttgag acaaaccgcc
     5941 gcagatttcg ggtctgggcg gtttgctttt cagacggcag ggcggtcagt tgccgtccag
     6001 ccagcggtcg cgtgtggttt tggcttcttc aaaatagcgg tacagggctt cgcggtcgtc
     6061 ggcggtcagg atgtctgcta aaacgtccaa ctgtttgccc aagccttgaa ccagttgcag
     6121 caggctgtct ttgttggcaa ggcagatgtc cgcccacacg gcgggatggc cggaggcgat
     6181 gcgggtgaag tcccgaaagc ccgtggcggc gaatttcaga tattcctgtc cgtcggggtg
     6241 gtcgagaatc tggtggacat aggcgaaggc ggtcaggtgg ggcatatggg agacggcggc
     6301 gaaaaccgcg tcgtggcgtt gcgcgtccat cgtataaatt tccgcaccga ccgcgtgcca
     6361 caggttttct atcaaggcaa tgccgtctga atgttcgccg ccgtgtggcg tgatgatgag
     6421 ttttctgtgg cggaacagcc cgaactgcgc ggcttgcgca ccgcttctgt ccgaaccggc
     6481 aatcgggtgg gtggcgatgc agtggtgcag gcggtcgggc agacagcggc ggaaggcttc
     6541 gatgaccgaa gatttggtgc tgccgacatc ggaaatccaa gtgtgttccg gcaaaacggg
     6601 gcgcagcgcg gtcaaaatgg caggaacggt ggcgacgggc gtggcaatca gtaccaagtc
     6661 cgcaccgccg atgctgtccg cgtcgatggc aacggaagcc cggtcaatca cgccgcgttc
     6721 caatgcacgt tcgaggttgt cgcggtcggt gtcgataccg gtaacggtgc ggacgagtcc
     6781 ctgccttttg aggtcgagaa cgaacgaacc gccgatcagc cctacaccga tgagggcaat
     6841 atggttcaaa atgggcattt gtgtaaacgg ttttcgcaaa gtaccgtcat ggtagcctat
     6901 cggcggaata tgccgcaagg tcggcaggaa aaaggagaag aaatggacaa aatcagagtt
     6961 gccgccgtgc agatggtgtc gggcgtgtcg ccggaaacca acgtcgccgc catgaaacgc
     7021 ctggtcgcac gggcggcgga gcagggggcg gattgggtgc tgctgcccga atattgggtg
     7081 ttgatgggcg caaacgatac cgacaaactc gcgcttgccg agcctttggg cggcggacgc
     7141 tttcagacgg cattgagcga aacggcgaaa gaatgcggcg tggtgctgtt tggcggaacc
     7201 gtgccgctgc aaagcagtga ggcaggcaag gtgatgaata cactgttggt gtacggacgg
     7261 gacggcgtaa gaacgggtct gtaccacaaa atgcacctct tcggcttttc cggtttgggc
     7321 gaacgctatg ccgaagccga taccatccgc gcgggcgggg atgtgccgca tctgtcggcg
     7381 gacggcgtgt ctgtggcggc aggtatttgt tacgatgtcc gctttcccga atttttccgc
     7441 cgccaactgc cgtttgacgt attgatgctt ccggcggcat ttacccacac gacgggcaag
     7501 gcgcattggg agttgctgct gcgcgcgcgt gcggtggaga accaatgtta tgttgtcgcg
     7561 gcggcgcagg gcggattgca cgaaaacggc aggcgcacat tcggacacag tatgattgtc
     7621 gatccgtggg gcgacgtgtt ggacgtattg cccgaaggcg aaggcatcgt tacggcagac
     7681 atcgatgcca accgactgaa cagcgtccgc aaccgcctgc ccgccttgaa ataccgggtt
     7741 ttggatgccg tctgaaggtt cagacggcat cggtgccggg gaatcagcgg gctgtaggat
     7801 gggcttcagc ctgccatttc catcaatccc acatttctac cgttttcatc gaatccatcg
     7861 aatccgcccc tttcgacgcc ccagtcctac gcaactgaac cgtcattccc acggaagtgg
     7921 gaatctagaa tctcggggtt tcagtcattt ccgatagatt cccaccgcgt caggggtctg
     7981 gattcccgcc tg
//
LOCUS       DAWGJV010000093         7887 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWGJV010000093 DAWGJV010000000
VERSION     DAWGJV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.093"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(51..2093)
                     /gene="recG"
                     /locus_tag="WHQ94_001443"
     CDS             complement(51..2093)
                     /gene="recG"
                     /locus_tag="WHQ94_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ2128705.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WHQ94_001444"
     CDS             complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WHQ94_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ2128706.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WHQ94_001445"
     CDS             complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WHQ94_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ2128707.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3113..4606
                     /gene="rng"
                     /locus_tag="WHQ94_001446"
     CDS             3113..4606
                     /gene="rng"
                     /locus_tag="WHQ94_001446"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ2128708.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4641..5885)
                     /locus_tag="WHQ94_001447"
     CDS             complement(4641..5885)
                     /locus_tag="WHQ94_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ2128709.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGPGLSIADTLAKKYGGHLEL
                     TDSRRFGHGLLIRALLDKETLK"
     gene            complement(5881..6056)
                     /locus_tag="WHQ94_001448"
                     /pseudo
     CDS             complement(5881..6056)
                     /locus_tag="WHQ94_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6279..7592)
                     /locus_tag="WHQ94_001449"
     CDS             complement(6279..7592)
                     /locus_tag="WHQ94_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ2128710.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1849 a         2342 c         2052 g         1644 t
ORIGIN      
        1 aaatcagatt tccgttacac ttttttccaa tatttcagac ggcattttgc tcacacgccc
       61 aaataccctt ccctgccgga aagccacctt gccaaatgcg cttcgacgat ttcggggttt
      121 tgttcaatca gcatcggggc ggtttcgcgc gcttgttcca aaaggtgtaa gtcttcttcg
      181 agcttggcga aacgcagcat aggcacgccg ctttgacgcg cgccgagaaa ttcgccgggg
      241 ccgcggatgt tgaggtcttg gcgggcgatt tcaaagccgt cggtgtgttc gtagatgact
      301 ttcagccgcg ctttggcgag ttcgcccaag ggttcggcaa acaggaggac gcacacgctt
      361 tccgccgcgc cgcgccctac ccgtccgcgt aattggtgaa gctgcgccaa gcccatgcgc
      421 tcggcgtgtt cgatgaccat cagggcggca ttgggcacat ctacgccgac ttcgatgacg
      481 gtggtggcga ccaagacgtt cagccccccc gaagaaaacc gcgccatcac ttcggccttt
      541 tcggcggcct tcatgcgccc gtgtaccagt ccgatattga gttcgggcaa tgccgtctga
      601 agccgggcga gggtttcggc ggcggtttgc agttgcaggg tttcgctttc ttcaatcaat
      661 gggcagaccc aatacgcctg ccgccctttt cggcaagtgc cgaggacgaa gccttcgact
      721 tcggcgcggc ggacgttgtt gacgaggcgc gttttaatcg gtgtgcgccc gggcggcaat
      781 tcgtcgatga cggacacgtc caaatcggcg aaaaaactca tcgcaagcgt gcgcgggatg
      841 ggcgtggcgg acatcatcag ctgatggact tcgcgccctt tgtttttgag ggcgaggcgt
      901 tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca agcccaaatt gtgaaacgcc
      961 acgccgtctg aaaacagggc gtgcgtgccg acggcgattt tgacgctgcc gtcagcgagt
     1021 ttggctttgg cttcgtcttt ggctttttta cgcaaactgc caaaaaggcg gacaacttca
     1081 atgcccaaag gttcgagcca ttgtttaaat ttaataaaat gctgttcggc aaggatttca
     1141 gtgggcgcca ttacagccac ctgcgcaccg gattcgatag ccgtcaaagc agacaaagca
     1201 gccacaatgg ttttgccgct gccgacatcg ccctgcagca ggcggtgcat cgggtaggtt
     1261 tgcgccatat cgcggcagat ttcggaaaca actttttctt gcgcatcggt cagggcaaac
     1321 ggcagggctt ggcgcagggc ttgggtcaat gtgccgtcgc cgcccaatgc cgccgccgtg
     1381 ccgccgatac gcttctgtcg cgccaagcgc atcgaaagct gttgcgccaa aagttcatcg
     1441 aatttgagcc gttgccatgc aggcagcgtg ccgtctgaaa gctgatgaat cgtgaaactc
     1501 ggcggcggcg aatgcaaaag acgcaggctt tcggcgaggt gtggcagctt cagacggcac
     1561 agcagggcat cgggcagcgt gtcgtgcagc ggcgtaacgt ccaacgccgt ctgaataata
     1621 cggcgcaaag tgggctggtt caaaccgttt acggtcgggt aaaccggcgt gaggctttcc
     1681 gccaaaccgc cgccctcggc atcgcggatt ttgggatgaa tcatctcgtc gccgtaaaag
     1741 ccgtgtttga tttcgcccac ggcgcggatg cgtttgccga ccgccgtctg tttctgatgg
     1801 ctggcgtaaa agtggatgaa gcgcagaaaa aggacgctgc cggaaccgtc ggcgatttgg
     1861 acaatcaact gcttgcgcgg tttgaacgtt acttcctgat ggataacctc gccctcgacc
     1921 tggcacggca cgccaatcgg cgcgtcctta atcggcataa tgtgcgtctc gtcctcgtaa
     1981 cgcagcggca ggtgcaacac caaatcccac gcggtatgga ggttgagttt gtcgagcttc
     2041 ttggcggaaa catcggtgat tttgagctgt ttctgggttt cgggcgacat cataggcaga
     2101 ttcctttgaa cgcacctatt ttatccgaaa acaaaaatac cgtctgaaac ggattcagac
     2161 ggcatcggca ggcaggaatc aagccccggc ggcttcggct tcctgctgtt gttggtaaat
     2221 cgcctcaaaa ttaatcggcg cgagcaggac gggcgggaag ccggcacgtg ttaccgtacc
     2281 ggaaacggct tcgcgcgcgt aagggaagag gatgttcgga cacgccacgc ccagcagcag
     2341 ctgcacatct tcttcgggga tgttttccaa acggaaaata ccgctttggg ttacttcgtt
     2401 caaaaacatc gtgcgctcgt tatccaattt ggcggttacg gtaacggtta catccacgtt
     2461 gtagtagccg tcttccagct tttggctgcc ggtggaaacg cgcatatcca cttcgggctc
     2521 gccctgttcc aaaaagattt gcggcgcgtg cggcacttcc aaagacaagt ctttgacata
     2581 cagtcgctcg atgctgaata cgggttgcag ttcttcgctc attttgtttt cctagttggg
     2641 ggttaagggt tcagcagtcc gtccagcccg ccttcctgct ggaggcggta gaggtcggta
     2701 aatccgccga cgtgcgtttc gccgatgaaa atctgcggca cgctgcgctg tcccgaaagc
     2761 tgctgcattt cggcaaaggc ttcggggctt gcatcgacac ggatttcgtc gatatgtccg
     2821 acacctgccg cgtgcagcag ccttttcgcc atcgcgcagt agggacaaaa cggacctgtg
     2881 tacatggtaa cggtctgcat attgggtttc cgaaagtttt gcaatgataa tcaatatagg
     2941 ggcatttccc ctgtttggca agtgcggaac agatgcacgt tcaaacggca tgtgcggaat
     3001 gtgtcaaagt ttctttttta aagtatgata gacattgtga aaaatatttt tgcacccgcg
     3061 ctgcgcggcg gaacggatgc aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     3121 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     3181 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     3241 gcgcaacagc gaacacagcc tagtcggcaa tatctatttg ggcgtggtgc gccgcgtgct
     3301 gcctgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     3361 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     3421 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     3481 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     3541 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     3601 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     3661 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     3721 catccaagaa caggcgaaaa tccggccgcc ggaaaccctg ctttatcagg atttgccctt
     3781 gagtttgcgc gtgttgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     3841 caccgtaaac cacggccgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     3901 caggatagaa ttgttcaaag gcgaacgccc gctatttgaa acccacaacg tcgagcagga
     3961 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     4021 atccaccgaa gcgatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     4081 tttcgatgaa acgattttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     4141 gaggctgcgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cgcaggaaag
     4201 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     4261 taccctgcac ggttttacca gcctgggact ggtcgagctg acgcgcaaac gctcgcgcga
     4321 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     4381 aacgccgcaa accgtatgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     4441 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     4501 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     4561 cgaaaccgct tacacgcagg aacaatacga catcgttttg atgtaaaaaa tgccgtctga
     4621 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaacgc ccgtatcaac
     4681 agaccgtgtc caaaacgtcg gctgtcggtc aattccaaat gtccgccgta ttttttggca
     4741 agcgtgtcgg cgatggacag ccccggcccc tgctgctccg ttcccaaaat acggtaaaac
     4801 ggatcgagga cgcgggcgcg ttcggattcg ggaatgccgt tcccgttatc ttccacccac
     4861 acggcgagat atttcccttc gtccgtgaaa cccaaatcta tcctgccttc gggcggcgtg
     4921 taacgtaccg cgttgtcggc aaaggtttta atcagcgtat agatttccgt ttcgtcggca
     4981 gacacttcga catcgcctcc gaccgccacg ccgatgtcct gacgtttttc caaagccagc
     5041 ggcatcagtt cctgcaacac ttggcggaaa cggctttgca gaccgaatgt cgttttcgtc
     5101 agaggggttt catccgactg cgaacgcgcc agtgccaaaa gctgttcgag caggtgtttg
     5161 ttgcgcctga tgctctgctg caaaacggca ggctgccgcg ctgcatcggg tgggagcgac
     5221 atattgttga gccgttccgc ctgaagggaa agggctgtca tcggcgtacg caattcgtgt
     5281 gccgcgtcgg cgacaaaccg ctgacggtgg cggatgtctt catccgcacg tttcaaaagc
     5341 aagttgatgg cggttacgaa ccctctgatt tcactgggaa tattgtccac actcaaggca
     5401 gacaggtcat tgattcggcg ttgttcgaga ctttgcgaca atttgcggac ggggcgcatg
     5461 gctttgtgcg taatccacac ggtcagcagt atcatcagcg gcagtgccgc cagcaggggc
     5521 aacacgcttt gccgtgccgc atccgccgcc aaatcttccc ggtattcgct ttcctgcatc
     5581 acggcaatcc gcccccgctc cgtcgtgcgg atatagacgc ggtaataatc gtcgtccccg
     5641 tccgcctgaa gcgtgtgcag accgtccgcc agatgcgcag gcaggctgac aacagggtct
     5701 tcctgctgcg gcatctgcac gaaaatgcgc gtatcgccgt cgccctcggg caaagtttcg
     5761 ggtttagaat cgggtgcgac gtacaatgcc gcctgacgga gcaggtcgtc ctgcaacgct
     5821 tccgtttcgt ggaaggtttc gtagtaggaa aacatacctg caagcattgc cagcggaaca
     5881 aacatccaaa ccgtcccgcc ccggcaaacc caaatccagc agcatcaagt cataaggctg
     5941 ggcagcggca gccgcgcgcc gtttttgacc caatccaccg catagccgcc gtctttcaaa
     6001 cttgccgaca ccgcctccgc aatcatcgca tcgtcttcca ccagcaaaac acgcatcaac
     6061 tttcccttca aaataaaccg tgcctattct aacaccccaa aattagccgc aatttagcgg
     6121 cctttacgct tgccggtatt tttcaaaact gcagcacaaa aaaaccgcgc cggcaactgc
     6181 cttcagacgg cattggggcg cgattgcaac acacgggcag gcaggcagag cctgcgacag
     6241 accacaggaa cgattcgggc ttcagacggc ttcgccgttt acggcagagg cacgattcct
     6301 gccgctatcg aactggccaa tatcgccagc gacaaacccc acgcccagaa aaacgaataa
     6361 cggatgtgtt tgcccatcga caatttcgcc aaacccaagc ccatccacaa agccggcgaa
     6421 agcggcgtaa caaaagtgcc gacgatactg ccgatcaaca tcgcataacc tgctgcttcg
     6481 ggcgccacgc ccgcctgcga ggtaatctgc tccacaatcg gaaacagtcc gaaataataa
     6541 gcgtccgtac tcaaaaccaa ctcaagcgga atgcccaaca caccgatggc aatatgcaga
     6601 taaggcagca gcgcgtccgg caggatatgc acaatgtctt tggaaatcgc gtccaacatc
     6661 cccgcaccct tcaaaatccc caaaaacgta cctgccgcca aaataatgga cgccatcatc
     6721 accgcgccgc cggcgtgggc ataaatccgc tccatctgtt cctgcgggct gcggtaattc
     6781 aaaagcaacg ccgccgttgc agccagcata aatacataac ccggtgggaa gatgcccgaa
     6841 aaaagcaggc tcatcgccgc caaaaacagc aggacattcc accaaaacag tttcggacgc
     6901 gccaattttt gttcttcttc cgacaaaggc accggcttta tcaaatccgc cacggcgggc
     6961 aacgcgccca actcccggac aatccgcctt ttttcacgca cacccaaaag cagggacagc
     7021 gcaaggataa acaccacacc gataatttgc accgtcaaca aaggcttata caattcgccc
     7081 acatctgcgc ccaacacgct tgcaacccgc ccggtcggcc cgccccacgg cagaaggtta
     7141 atcaatcccg cactggaagt cagcagcaaa aacagcaggt aaggattcat atgcagacgc
     7201 ttgtaaagcg gcaaaagggc ggggacgacc aataaaaacg tcgtcgcacc cgccccgtcc
     7261 aactgcgcca ccaccgacac caagaccgtc cccacactca ctgccacgat attaccccga
     7321 gtcagcttaa tcaaaccgcc tatcatcgga cggaacagcc ccacatcgtt catgattcca
     7381 aaaaacaaaa tggaaaacat aaacataatc acaatctgca tcaccgattt ggtgccgccc
     7441 gaataaaatt cttttaattg ggatacatca aaccccgcca gcaacgcccc aaacagcggc
     7501 accaagatta atgcgatgat gggcgacact ttttccgtca gcagcaacca tacgatgacc
     7561 ccgataatca gcagtccgat aaacgtcagc atcatttctc ctttatttta ttttaaacag
     7621 aaaaccgacc gtgcaggcaa aaccgcccac agacgcggga taagccctgc attctacttt
     7681 tttattttga aacaagtcaa tcggtcattt cctcccattc acgcctgccg ccattcctgc
     7741 atccgtccgt catttcacgg cggcaaccga tacggaacaa ccggcaaatc ggtatcggga
     7801 cggcgcgggg gcattcatcc cggtgcgccg attcaaacga aaccgcccct atcattgcgg
     7861 agcgcggggc gtgccgtaca cgcggga
//
LOCUS       DAWGJV010000094         7863 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWGJV010000094 DAWGJV010000000
VERSION     DAWGJV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.094"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..1337)
                     /locus_tag="WHQ94_001450"
     CDS             complement(<1..1337)
                     /locus_tag="WHQ94_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ2128711.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILSNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1399..2748)
                     /locus_tag="WHQ94_001451"
     CDS             complement(1399..2748)
                     /locus_tag="WHQ94_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ2128712.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRTEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLQLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRREIVSAVSPKIILNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2891..3517
                     /locus_tag="WHQ94_001452"
     CDS             2891..3517
                     /locus_tag="WHQ94_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ2128713.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3581..4999)
                     /locus_tag="WHQ94_001453"
     CDS             complement(3581..4999)
                     /locus_tag="WHQ94_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2128714.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            5326..6054
                     /locus_tag="WHQ94_001454"
     CDS             5326..6054
                     /locus_tag="WHQ94_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ2128715.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6113..6601)
                     /locus_tag="WHQ94_001455"
     CDS             complement(6113..6601)
                     /locus_tag="WHQ94_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ2128716.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7359..7823)
                     /locus_tag="WHQ94_001456"
     CDS             complement(7359..7823)
                     /locus_tag="WHQ94_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ2128717.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2022 a         2119 c         1981 g         1741 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcaat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcagatcga gggcgcgggc ggcgagcagg
      241 gtcagggttt cggggtcggt gtgcaaggtt tggcggccgg cgagtttgtc ggaaatggtg
      301 cgggtattgg acaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccggttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcaat taaaaagtcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aattcatccc gatatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcaccg tggctgccga gaaactgttg cagccgctcg
     1081 atgccgtctg aacgcgtatc cgtatgggtt tccagccatt ccaaaacacc gcccgcgtct
     1141 tcgagtccgg gcgtgtcata caggaaaacc agcatgtccg cgccgtcgct gatggcggct
     1201 tcttcgacat ggcgcgtagt ggacggggca tttttgactt cgccgaagcc gctgtcgcgc
     1261 aatagggtgc gcaggagcga ggttttgccg gtattggtat gcccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacgacct tttaaggatg
     1381 gcggctttat ggggcgtttc aagtgcggtc gttggttttc agacggcctt cctgcgccgc
     1441 cctgtcaggc tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag
     1501 cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc
     1561 ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg gcacagtttg
     1621 ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcgcggc
     1681 aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc
     1741 gaaccattcg ccgtcctgcc actcggtctc cagcatgacc gcccatttcg gtgcatcgtt
     1801 caagatgatt ttcggtgaaa cggcggacac gatttcccga cgcgtatccg catcggtgat
     1861 tttgttctgc cagcggcgga tgaccgcctg ataatagggc ttttccaaat ccaagccgtt
     1921 ctcgcttgtt ttcaaaagga ttttacacac tacccaagcc agcaggcgcg gcaggatgcc
     1981 gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc
     2041 gttcagacga ccttcgatga ccgctcgcgc atcaggcacg ggaaaaccca gtttcgcagg
     2101 cagccatgcc aacatttcca ccagccgtac cgaagacgaa tcgcccaaca gcgtgctttc
     2161 ccagttgaac gtatattgcc gcaccaaaag cagcaacaat accgacacca gcattccgag
     2221 cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta ccgaaggttg
     2281 ccgccactcg tccgcataca gctgcaacac cgcctgattg acagggtctt tgccccgaaa
     2341 ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca acattgccaa
     2401 ccatactgct aacatcagcg tattcatgcc caacacgccc gccaaaatca ggaagaaatt
     2461 cagcccctga ttgtccatca gaagataagt gcctgaaaat ccggcggtaa acatcatcga
     2521 tgccgccacc acccacaacc agaacgaccc cacacgcaca cgttccaacg tgtcccgcag
     2581 catacggttc ctgtcgatca tctccgtccg acggatgatt ttttcctccg tactgccgtc
     2641 cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa agccgccttc
     2701 ttccaaaata cggaccagct caaccagttt tcgggatgga ttcaacataa aatgccgtct
     2761 gaaaataaaa aacagatttt aacacacgaa ttttcaagaa tattcacagt gtaggcaaag
     2821 agtaaatccc acacagaggc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa
     2881 agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga gcggtttgat
     2941 tctggcggcg gcaggtttgg tgctgatgct gctgaaaacc ataggacacg gggacggcta
     3001 ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc
     3061 gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg
     3121 catgatttat gtcctgattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa
     3181 cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca
     3241 agaactcacc atcggacgga aaagcgaaaa acgtctgctg tctattgtga tttatgtcgt
     3301 catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgtcggcagg
     3361 actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa
     3421 cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat
     3481 cacccaattt gtcagtgtgt acggttatgt aatctgaatg ccgtctgaaa aacaaaacct
     3541 cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg tcgtggaact
     3601 cgtggaattc atacggataa gacaaatccc gacccgcttt tttctgtgcc aaataatcat
     3661 cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc gggataacca
     3721 taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg agcgtaccca
     3781 aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc ctgaaaagaa
     3841 aacggatgtt ggactcgccg aaataatacc agccgataat ggtcgtcaag gcgaagaagg
     3901 tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta aaggcaaatt
     3961 gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc ggtcagcagg ataatcaatg
     4021 ccgtagccgt acataccaaa atcgtatcga taaatacacc gacaaatgcc gccatacctt
     4081 gctgcacagg atgcttcaca tccgcagtcg cgtgggcgtg cggagtcgaa cccatacctg
     4141 cttcgttgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcgcgc ataccgatac
     4201 ccgccgcacc gcccaaaacg gcttcgggat tgaaggcggc ggtaaagatg tggttgaaca
     4261 tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa atataaacaa
     4321 ccgccataaa cggcacgaca aattgggcaa tattggcaat acggttcacg ccgccaatca
     4381 caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc acatcaaatg
     4441 caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata aagcccaatg
     4501 cgataatcaa cgcaatggaa aagaaaccgg acaaaaaacg cgccgctccc ctgccgattt
     4561 tcggagtcag accgtgggtg atgtagaacg ccggcccgcc gatgtatttg ccgtggctga
     4621 cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc cccaaaacgg
     4681 cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg gtcgccacgc
     4741 cggcgacgtt gcccgtaccg atttgcgcgg atatggcaac cgccaacgcc tgaaactgcg
     4801 ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg gatttgaatc
     4861 ccgcgcccag cttggtaatc tgcggcgcac caagatacag cgtaaaaaac aggccgatac
     4921 ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta cccaccagaa
     4981 cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac tgccttccaa
     5041 aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca gatgccttaa
     5101 attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt aattaaaaca
     5161 tttctttatt tttaagcgga aagcggagga aatcgctttc agacagcata gacaacggca
     5221 cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg accgtaaaaa
     5281 atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc aggccatagc
     5341 aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg caaaatcttc
     5401 acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga ccccggtgcc
     5461 aacccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc caaagacaat
     5521 gtgcaacgcg ccatcgataa aggtacgggc aacttggaag gcgtggaata catcgagttg
     5581 cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct gaccgacaac
     5641 aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg caacttgggt
     5701 accgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt cgaacccggc
     5761 gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga cgtggttacc
     5821 aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg cgtaaaatcc
     5881 gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg cgcccaaaac
     5941 gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga cgcgctggaa
     6001 gacttggacg acgtgcaaga cgtttacact tccgccgtat tgaatctgga ctgatacgca
     6061 gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg atttatttag
     6121 cccttgccca cgcccaccaa accgtcagga caaccgccag aaaatacgcc gcgctccaaa
     6181 caggcaaagc aacccctaac agataatccg gttcggcaca atttccgaac ccgcgcacga
     6241 caggctcgaa ccaatcaaac aaagaccagc ccttcaagcg gaacgtccac ggcgcaccgc
     6301 acgaaggagc cgtacccggc ggcagcgact gcaaccacaa ctgatatgcc gcaacagaaa
     6361 tacccgtaac ggccggaatg ctgataaaga cagcaccgaa caaaccgccc gcccttcttc
     6421 ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg cataaccgct
     6481 gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc aaagaaccgg
     6541 caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat aacggggtca
     6601 ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat atcccgagat
     6661 ggcaagggac gaatcgacac cggcgcaggt gcggacgatt tcggttttcc cgatatacac
     6721 aagacctgac aagggacaaa acggcgattt gcccgcacaa tgcccgcaaa aaaatacatc
     6781 cggaggattt gaatttcggc aaatttagcg aatcaaaagt ttatttcaat gaaattatat
     6841 gatttttttg aataagcgga ttgacaggtt tttgaaggaa tttgttaccg gatagccatc
     6901 gggcaagttt tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat aattgacagc
     6961 ggataagaaa cggcggataa ttctcgccgt cgagttgctt gatgcagctt gatttttctc
     7021 ctctatttct cctttgtaga cttggcacac attcaaccgg atgtgtgcat ttttttatct
     7081 ccgctttttt tgaaatttaa ttacttttat tgtttgaaat tccattcttt aagaaattta
     7141 acaagaaaaa cgcttgcttt ttgaccggaa aagctgcata attcgttccg ttagctggtt
     7201 cgagtagtca gttaatagtt tctcctctat ttctcctttg tagacttggc acacattcta
     7261 ttgaatgtgt gcattttttt atctgaagca acaagcctct gtgcgtgatg ttgttatgtt
     7321 tcatttaggt gtcaaaccgc atatccggtc tgaaatattc aatccaaatc caaaaccgga
     7381 ttttctttga cctcctccat cacaacataa ctcctactct ccgaagcggc aggcagttgc
     7441 aataggatat tgcctagcat atcccgatag gcagacatat cgggcaaacg tactttaatc
     7501 agatagtcgt attcgcccga caccaagtgg cattccataa tttgcggaat tttcagcact
     7561 tcttttttga aatcttcgaa aatattgccc gatttggatt gcagcttcag ctcgacaaaa
     7621 accaatagtg gtttgcccaa cagatgggga ttgaggtggg cgtgatagcc ggaaatataa
     7681 tgttcccgct ccaaacggcg caccctctct gtaacgggcg tggtggacaa gcctaccttc
     7741 tcggcaagct ccgtcatcgg gatgcgggca ttctgttgaa ggatcttaag aatgcggaaa
     7801 tcgattttat ctagttcttt catttggatt gccttgtatt tattattgat tttaacaaat
     7861 aga
//
LOCUS       DAWGJV010000095         7790 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWGJV010000095 DAWGJV010000000
VERSION     DAWGJV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.095"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WHQ94_001457"
     CDS             complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WHQ94_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ2128718.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPSELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1588..1743
                     /locus_tag="WHQ94_001458"
     CDS             1588..1743
                     /locus_tag="WHQ94_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128719.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKIAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1740..2170
                     /locus_tag="WHQ94_001459"
                     /pseudo
     CDS             1740..2170
                     /locus_tag="WHQ94_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
     gene            2304..6695
                     /gene="app"
                     /locus_tag="WHQ94_001460"
     CDS             2304..6695
                     /gene="app"
                     /locus_tag="WHQ94_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ2128720.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYTDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKNWFYDEIFTGDTHSVFYEPHQNGKYTFHD
                     NNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNRHSLSFHRIQNTDEGAMIVNHNATTTST
                     VTITGNESITQPSGKNINRLNYSKEIAYNGWFGEKDATKTNGRLNLVYQPAAEDRTLL
                     LSGGTNLNGNITQTNGKLFFSGRPTPHAYNHLNNHWSQKEGIPHGEIVWDNDWINRTF
                     KAENFHIQGGQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCV
                     ENTITDDKVIASLTNTDISGNVDLADHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQ
                     RGNLSLVGNAQATFNQATLNGNTSASGNASFNLSNNAVQNGSLTLSGNAKANVSHSAL
                     NGNVSLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITL
                     NSAYRHDAAGAQTGSVSDTPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGT
                     FRFMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLN
                     FTLQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDA
                     LIAAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPA
                     TTAFPRARRARRDLPQPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDE
                     LDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSH
                     NRTENTFDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRV
                     LHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKAD
                     YSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFT
                     LSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            complement(6804..7526)
                     /locus_tag="WHQ94_001461"
     CDS             complement(6804..7526)
                     /locus_tag="WHQ94_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ2128721.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGADEDIDFPDPPNMG"
BASE COUNT         2079 a         2180 c         1875 g         1656 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaaccaaac ggatcggatt cccgttttta
       61 tgggataacg gaatgttcag ccggacaccg ccacgatgct ttcgatccgt ttattttccg
      121 atacaaaacc tcgaaaaaat cacgccgagc aggtcgtccg ccgtaaactc gcccgtgatt
      181 tcgccgcatg cgacctgcgc caagcgcaag tgttcggcaa acagctcgat ttgatggttg
      241 ccgcacaatg ccgccagcga caattcttcc tgcgctgctt tgagtgcgtt gacgtgccgc
      301 gtccgcgcca aaaacagccc ttcgccctcg ccctgccatc cggcctcgcg caacaacgtc
      361 cgtttcagcg cgtccaagcc gtcgccagtt ttcgccgaca gcgcgatgac ggtttccgcg
      421 cccgtaccga acccgcctgc cgcgtgtgca tgcaaatcag atttgctgtg gatttcgatg
      481 cgtttcaact ccgacggcag cgcgtccaaa atcgcccgtg tcttttcatt caaaccctcg
      541 cgcggatcga ccaacaccag cgcgacatcg gcttcggata cggctttgcg gctgcgttcg
      601 atgccgatac gctcgaccac gtcgtccgtc tcgcgcaaac ctgccgtatc gacaatatgc
      661 accggcacgc cgtcaatcag gatacgttcc ctgactgcgt cgcgcgtcgt tccggcaata
      721 tcggtaacaa tcgccacttc gtcgcccgcc agagcattca gcaggctcga cttgcccaca
      781 ttcggcgcac cgaccaatac gacattcaga ccttcgcgca aaatcgcgcc ctgctgtgcg
      841 ttggcaagca catcatccac ggcgcggcgc aagccgtcca gtttgccgcg tgcgtccgcc
      901 gcttcgagaa aatcaatgtc ttcctcggga aaatccagcg tcgcttcgac cagcatccgc
      961 aaggtaatca aatcttcgac cagaccgtgt atccgccgcg aaaaatcgcc cttgagcgag
     1021 cgcaacgcca gacgcgccgc cgaacggctg gatgcgtcaa tcaaatccgc cacaccttcc
     1081 gcctgtgcca agtccagttt gtcgttcaaa aacgcacgtt tggtaaactc gcccggttcg
     1141 gcaaggcgcg cgcccagttc caaacagcgg ttcagcagca tatccatcac caccggcccg
     1201 ccgtgtccct gaagctcgat gacatcttcg cccgtaaaac ttgccggtgc ggcaaaaaac
     1261 agtagcagcc cgctgtcgat tgcctgtccg tccgcgtccg taaaatcagc ataggttgcc
     1321 acgcgcggct tgggcgtttt cccgcacaaa gcctgcgcca tcggcagcag gtttttcccc
     1381 gatatgcgta tcacgcccac gccgccgcgc cctggtgcgg tagcgactgc cgcaatcgtt
     1441 ggaacgttat ccgacataaa acccccgaaa attcaaaaca gctgcgatta tagcaaatgc
     1501 cgtctgaagt ccgacggttt ggctttcaga cggcataaaa ccgcaaaaat gcttgataaa
     1561 tccgtccgcc tgacctaata taaccatatg gaaaaacgaa acacatacgc cttcctgctc
     1621 ggtataggtt cgctgttggg cctgttccat cccgcaaaaa tcgccatccg ccccaatccc
     1681 gccgacgatc tcaaaaacat cggcggcgat tttcaacgcg ccatagagaa agcgcgcaaa
     1741 tgaccgaaaa cgcacaggac aaggcgcggc aggctgtcga aaccgtcgtc aaatccccgg
     1801 agcttgtcga gcaaatcctg tccgacgagt acgtgcaaat aatgatagct cggcgtttcc
     1861 attcgggacc gttgccgccg ccgtccgact tggcgcaata caacgacatt atcagcaacg
     1921 gggcagaccg cattatggca atggcggaaa aagaacaagc cgtccggcac gaaaccatac
     1981 ggcaagacca aaccttcaac aggcgcgggc aactgtacgg cttcatcagc gtcatcctga
     2041 tactgctttt tgccgtcttc ctcgtatgga gcggctaccc gcaaccgccg cctcccttgc
     2101 cggcggcaca gtggttgcct tggcgggtgc tttcgtgatt ggaagaagcc gagaccaagg
     2161 caaaaattaa tgacaaatcc tagggcgtgc ttcatatccg cccgaacgcc gaaccgcaca
     2221 tataggcaca tcccgcgcgc cgcctggaag cggaagccgc gccctcccaa acaaacccga
     2281 atcccgtcag ataaggaaaa ataatgaaaa caaccgacaa acggacaacc gaaacacacc
     2341 gcaaagcccc gaaaaccggc cgcatccgct tctcgcctgc ttacttagcc atatgcctgt
     2401 cgttcggcat tcttccccaa gcctgggcgg gacacactta tttcggcatc aactaccaat
     2461 actatcgcga ctttgccgaa aataaaggca agtttgcagt cggggcgaaa gatattgagg
     2521 tttacaacaa aaaaggggag ttggtcggca aatcaatgac aaaagccccg atgattgatt
     2581 tttctgtggt gtcgcgtaac ggcgtggcgg cattggtggg cgatcaatat attgtgagcg
     2641 tggcacataa cggcggttat aacaatgttg attttggtgc ggagggaagc aatcccgatc
     2701 agcaccgttt ttcttaccaa attgtgaaaa gaaataatta taaagcaggg actaaaggcc
     2761 atccttatgg cggcgattat catatgccgc gtttgcataa atttgtcaca gatgcagaac
     2821 ctgttgagat gaccagttat atggatgggt ggaaatatac cgatcaaaat aattatcctg
     2881 accgtgttcg aatcggagca ggcagacaat attggcggtc tgatgaagat gaacccaata
     2941 accgcgaaag ttcatatcat attgcaagtg cgtattcttg gctcgttggt ggcaatacct
     3001 ttgcacaaaa tggatcaggt ggtggcacag tcaacttagg tagtgaaaaa attaaacata
     3061 gcccatatgg ttttttacca acaggaggct catttggcga cagtggctca ccaatgttta
     3121 tctatgatgc ccaaaagcaa aagtggttaa ttaatggggt attgcaaaca ggcaacccct
     3181 atataggaaa aagcaatggc ttccagctag tgcgtaaaaa ttggttttat gacgaaattt
     3241 ttaccggaga tacccattca gtattctacg aaccacatca aaatgggaaa tacacttttc
     3301 acgacaataa taatggcaca ggaaaaatca atgccaaaca tgaacacaat tctctgccta
     3361 atagattaaa aacacgaacc gttcaattgt ttaatgtttc tttatccgag acagcaagag
     3421 aacctgttta tcatgctgca ggtggtgtca acagttatcg acccagactg aataatggag
     3481 aaaatatttc ctttattgac gaagggaaag gcgaattgat acttaccagt aacatcaacc
     3541 aaggtgctgg aggattatat ttccaaggag attttacggt ctcgcctgaa aacaacgaaa
     3601 cgtggcaagg tgcgggcgtt catatcagtg aagacagtac cgttacttgg aaagtaaacg
     3661 gcgtggcaaa cgaccgcctg tccaaaatcg gcaaaggcac gctgcacgtt caagccaaag
     3721 gggaaaacca aggctcgatc agcgtgggcg acggtacagt cattttggat cagcaggcag
     3781 acgataaagg caaaaaacaa gcctttagtg aaatcggctt ggtcagcggc aggggtacgg
     3841 tgcaactgaa tgccgataat cagttcaacc ccgacaaact ctatttcggc tttcgcggcg
     3901 gacgtttgga tttaaacagg cattcgcttt cgttccaccg tattcaaaat accgatgaag
     3961 gggcgatgat tgtcaatcat aatgccacaa caacatccac cgttaccatt acagggaatg
     4021 aaagtattac acaaccgagt ggtaagaata tcaatagact taattacagc aaagaaattg
     4081 cctacaacgg ttggtttggc gagaaagatg cgaccaaaac gaatgggcgg ctcaaccttg
     4141 tttaccagcc cgccgcagaa gaccgcaccc tgctgctttc cggcggaaca aatttaaacg
     4201 gtaacatcac gcaaacaaac ggcaaactgt ttttcagcgg cagaccaaca ccgcacgcct
     4261 acaatcattt aaacaaccat tggtcgcaaa aagagggcat tccgcacgga gaaatcgtgt
     4321 gggacaacga ctggattaac cgcacgttta aagcggaaaa tttccatatt cagggcgggc
     4381 aggcggtgat ttcccgcaat gttgccaaag tggaaggcga ttggcatttg agcaatcacg
     4441 cccaagcagt tttcggtgtc gcaccgcatc aaagccacac aatctgtaca cgttcggact
     4501 ggacgggtct gacaaattgt gtcgaaaaca ccattaccga cgataaagtg attgcttcat
     4561 tgaccaatac cgacatcagc ggcaatgtcg atcttgccga tcacgctcat ttaaatctca
     4621 cagggcttgc cacgctcaac ggcaatctga ccgcaggcgg agacacgcac tatgaggtta
     4681 cgcacaacgc cacccaaaga ggcaacctta gcctcgtggg caatgcccaa gcaacattta
     4741 atcaagccac attaaacgga aacacatcgg cttcgggcaa tgcttcattt aatctaagca
     4801 acaacgccgt acaaaacggc agtctgacgc tttccggcaa cgctaaggca aacgtaagcc
     4861 attccgcact caacggcaat gtctccctag ccgataaggc agtattccat tttgaaaaca
     4921 gccgccttac cggacaaatc agcggcggca aggatacggc attacactta aaagacagcg
     4981 aatggacgct gccgtcaggc acggaattag gcaatttaaa ccttgacaac gccaccatta
     5041 cactcaattc cgcctatcgc cacgatgctg caggcgcgca aaccggcagt gtgtcagaca
     5101 cgccgcgccg ccgttcgcgc cgttcgcgcc gttccctatt atccgttaca ccgccaactt
     5161 cggcagaatc ccgtttcaac acgctgacgg taaacggcaa attgaacggt cagggaacat
     5221 tccgctttat gtcggaactc ttcggctacc gcagcgacaa attgaagctg gcggaaagtt
     5281 ccgaaggcac ttacaccttg gcggtcaaca ataccggcaa cgaacctgca agcctcgaac
     5341 aattgacggt agtggaagga aaagacaaca aaccgctgtc cgaaaacctt aatttcaccc
     5401 tgcaaaacga acacgtcgat gccggcgcgt ggcgttacca actcatccgc aaagacggcg
     5461 agttccgcct gcataatccg gtcaaagaac aagagctttc cgacaaactc ggcaaggcag
     5521 aagccaaaaa acaggcggga aaagacaacg cgcaaagcct tgacgcgctg attgcggccg
     5581 ggcgcgatgc cgtcgaaaag acagaaagcg ttgccgaacc ggcccggcag gcaggcgggg
     5641 aaaatgtcgg cattatgcag gcggaggaag agaaaaaacg ggtgcaggcg gataaagaca
     5701 gcgccttggc gaaacagcgc gaagcggaaa cccggccggc taccaccgcc ttcccccgcg
     5761 cccgccgcgc ccgccgggat ttgccgcaac cgcaacccca accgcaaccc caaccgcagc
     5821 cccaaccgca gcgcgacctg atcagccgtt atgccaatag cggtttgagt gaattttccg
     5881 ccacgctcaa cagcgttttc gccgtacaag acgaattaga ccgcgtattt gccgaagacc
     5941 gccgcaacgc cgtttggaca agcggcatcc gggacaccaa acactaccgt tcgcaagatt
     6001 tccgcgccta ccgccaacaa accgacctgc gccaaatcgg tatgcagaaa aacctcggca
     6061 gcgggcgcgt cggcatcctg ttttcgcaca accggaccga aaacaccttc gacgacggca
     6121 tcggcaactc ggcacggctt gcccacggcg ccgttttcgg gcaatacggc atcgacaggt
     6181 tctacatcgg catcagcgcg ggcgcgggtt ttagcagcgg cagcctttca gacggcatcg
     6241 gaggcaaaat ccgccgccgc gtgctgcatt acggcattca ggcacgatac cgcgccggtt
     6301 tcggcggatt cggcatcgaa ccgcacatcg gcgcaacgcg ctatttcgtc caaaaagcgg
     6361 attaccgcta cgaaaacgtc aatatcgcca cccccggcct tgcgttcaac cgctaccgcg
     6421 cgggcattaa ggcagattat tcattcaaac cggcgcaaca catttccatc acgccttatt
     6481 tgagcctgtc ctataccgat gccgcttcgg gcaaagtccg aacgcgtgtc aataccgccg
     6541 tattggctca ggatttcggc aaaacccgca gtgcggaatg gggcgtaaac gccgaaatca
     6601 aaggtttcac gctgtccctc cacgctgccg ccgccaaagg cccgcaactg gaagcgcaac
     6661 acagcgcggg catcaaatta ggctaccgct ggtaacaccg gatatgccga aaggggtttg
     6721 acgatgccgc cgtgcgctgt caaacccttt ttctgccgcc gccttgtgcc tgctccattg
     6781 tctgataacc gtcaaattga tttttagccc atgtttggtg ggtcggggaa atctatatct
     6841 tcgtccgcgc cgaaatagtc tatgcccgat atacaatttt gatacacaaa cttggaaata
     6901 tcggtatcgt cacccgagcg atagaatgcg gacagttttt cattgaattg cggcatctgc
     6961 atttcggaga tttccaagat gccacggccc ctctccatca tcagaccatt ggcaaataag
     7021 gttgccgttc gtttgttgcc gtcccaaaaa acctgctgcc gcatacaata aagcataaaa
     7081 cggacggctg cctccgtcgt cgaaccgctt tgcagtccga tattttgtaa cacgcgagcc
     7141 acttcaattt ccttcaccgg atttggggca tgacgggaac cgtccaatag cgttacgccg
     7201 accgaaccgg tacggaaatc accgggtgcc aaagaatcgt ccttggcaac aatgttgttg
     7261 atttttttaa ggagtgaaat atcgaccggt tcgccgtttg aaatatgcga aatcacatat
     7321 tgataggcac gtttcaggtt caagatggtt tggatgtctt ccagtgacgc ggaggctaca
     7381 tttttgccat ggataatttg ttcggtctgc aacaaggtcg tctgacaatt ctcaaaacgg
     7441 ctgaggttgt gaatttgggc aaccaatact ttctttgcca aaaaaatatt ttctttcaaa
     7501 ctcaacttgt atttgtcggg aaacacgata attccttaaa taattaaagc agtacgttaa
     7561 atcatcatct tatcatcact ttcggggttt atgttgcatg cgtccgtttt ctaatacggt
     7621 ttcccatccc gcgaaacgag gcggaggcct gtgcaaaaaa cctgttcacg aaattttccg
     7681 cattaacgat acgctgaaaa tgcgctaaaa atgactgtgt ttgaatatcg gttgatttca
     7741 tccgtttgtg taacctcgcg ccggttttgt catgattttg cattatagtg
//
LOCUS       DAWGJV010000096         7699 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWGJV010000096 DAWGJV010000000
VERSION     DAWGJV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.096"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..325
                     /locus_tag="WHQ94_001462"
     CDS             <1..325
                     /locus_tag="WHQ94_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128722.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="WHQ94_001463"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="WHQ94_001463"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ2128723.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WHQ94_001464"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WHQ94_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ2128724.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="WHQ94_001465"
     CDS             complement(2119..2433)
                     /locus_tag="WHQ94_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ2128725.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="WHQ94_001466"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="WHQ94_001466"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ2128726.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WHQ94_001467"
     CDS             complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WHQ94_001467"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ2128727.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WHQ94_001468"
     CDS             complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WHQ94_001468"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ2128728.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5917..7560)
                     /gene="pgi"
                     /locus_tag="WHQ94_001469"
     CDS             complement(5917..7560)
                     /gene="pgi"
                     /locus_tag="WHQ94_001469"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ2128729.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSEQETARHFCAVSADTDAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEEGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKIFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
BASE COUNT         1711 a         2148 c         2074 g         1766 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaac cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg tgatttgaag
     1021 tggcagtttc ggcaccggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatatcgg catcggcttt ccaagccgga tccaaatcgc tgagtttcaa accaagcagg
     1141 cggtacacgc cgttgaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc acttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcacgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctgacggtaa
     1861 agcttgccta gggtgaggtt taggtcgtat gactgaggct gctgatcaat gacctctgcc
     1921 aattccctgg cggcacgtcc gctgttgcgg tcaaccaagg catccagact tttataaaat
     1981 cccgacggta tgctttttgc ctgctttaat acagtcttca tatccacacg ggcggcaaac
     2041 cagcccatcg cgaagaaaac gggcaaaagg ataatcggca gcaggataat ccacaattca
     2101 ttgtccatat cggctttctc aaggctgttt ggcagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tctttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccga acacgatgcc
     2281 gacgacaaac gcgccgaaca atacgacaat cagcggcaaa tcgactttct gtccgggcag
     2341 gtaggagaag gtaacggcat ccgtattaat gacggcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactaca gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg tcatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggctcgcccg tcaaaatttt agtggaaacc
     3241 gaacacatcc gctgcgcccc gcatatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatacggcg caaaccaagt cctcttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgaattt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaggcg attctgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaatgc cgtctgaaac ccgtttggcg
     3781 tttcagacgg cattttcgca tccgaaccgt ttccgccgca cctgcagcaa gtcggcacaa
     3841 aggcaatcgg ttaaaacaag cgtccgcatt tcccatcccg cctgccgtaa gtcgggcatt
     3901 tctctagaaa tacgcttcag acggcaaacc gccgcccgaa accgatatgt ggcacggacg
     3961 gcgcacggat ttgaaaacgg cggattatcc ctcggtgctc aaggcattga tgctgtaacc
     4021 gccgtcaacg taagtgattt cgccggtaat gccggacgac aggtcggaca gcaggaaggc
     4081 ggcggtattg ccgacttctt cgatggtaac gttgcggcgc agcggattgt gggcggcgac
     4141 gtgtcccaag agtttgccga aatcggcgat gccggaggcg gcgagtgttt tgatcggacc
     4201 ggcggaaatg ccgttgcagc ggatgccttc tttgcccaga caggcggcgg taaaacggat
     4261 gcccgcctca aggctggctt ttgccatacc catcacgttg taattcggaa tcgcgcgcac
     4321 cgcacccaag tagctcaggg cgacgatggc ggagtttctg ccgcgcatca tcggacgggc
     4381 ggcttttgcc agcgcaggca ggctgtatgc ggaaatttcg tgtgcggtgt tgaacgcttc
     4441 gcggctgatg ctgtcgagga aatcgccgct caaggcttct ttcggcgcga agccgatgga
     4501 atgcaccaaa ccgtccaagc cgtcccaatg cttgcccaag tcggcgaaca cttggttgat
     4561 ttcgtcgtcg ctggcgacat cgcaacggaa cacgagttcg gaatccaatt ccgccgccat
     4621 tttgcggaca cgctcttcca gtttgtccac aacgtaggta aacgccagtt ccgcgccttg
     4681 ttcgcggcag gctttggcga tgccgtaagc gatggaacgc tcggaaatca tgccggtaat
     4741 cagaattttt ttgccttgca gaaaacccat tttgtatcct tcaaatagtg ttcggttcat
     4801 tctaaaccgt tgattatagc aaattgtccc tgtttctgtg ttttcacgtt gcagcgtgca
     4861 aacggcaatg ccgtctgaag cggatttcag acggcatcgg acgtttcaaa tacggtttaa
     4921 ggcatcagat gccgcgcaac aattcgttca cactggtttt ggcgcgtgtt tgcgcgtcca
     4981 cacgtttgac gatgacggcg cagtaaaggc tgtggctgcc gtctttggaa ggcatactgc
     5041 cggatacgac aaccgaacct gccggtacgc ggccttggta gatttcgcct gtggtgcggt
     5101 cgaagatttt ggtggattga ccgatgaaca cgcccatcga aatcacgctg ccttcttcga
     5161 caatcacgcc ctcaacgatt tcagaacgcg caccgatgaa gcagttgtct tcaatgatgg
     5221 tgggtgcagc ctgcaggggt tcgagtacgc cgccgatacc gacgccgcca ctcaagtgca
     5281 cgtttttacc gatttgcgcg caagagccga cagtcgccca agtatcgacc atcgcgcctt
     5341 cgtcgacgta tgcgccgatg ttgacgtaag acggcatcag cacgacattt ttcgccacaa
     5401 agctgccgcg tcgggcaacc gcacccggaa cggcgcggaa gcctgcgttt ttgaactcgt
     5461 cttcagacca gtcggcaaac ttggtcggca ctttgtcgaa gtatttgttc acgccgtcgt
     5521 tgaggacttc gttgtcttgg atgcggaagg acagcaacac ggcttttttc gcccattcgt
     5581 tgactttcca ctcacccacg cccaagcgtt cggcaacgcg cagtttgccg gaatcgagtt
     5641 ggcggatggt ttccaacacg gcttctttga cttcgggagt aacggtggtc ggggtaatgt
     5701 ccgcgcggtt ttcaaaggcg gtttcgataa tgttttgcaa agacataatg tttccttatg
     5761 tgagatgttt cgtgggacgc ttgccggtat tttcaagcag tatgttcatt tgaaatgaat
     5821 gcgctattgt gccacgcaaa cgcatattcg tcagcaatac gcgggcggtt gccgaacatt
     5881 ggcgtttcgg aatcgggcgg gcggcaggta ttgcctttat gccgcgccgc cgcctttcag
     5941 acggcattcg cggtaaaacg ccatcagccc ttcggtcgag gcatcgtgta cggacgtgcc
     6001 gccttccagt tcgccgatga tggttttcgc caactgtttg ccgtactcaa ccccccactg
     6061 atcgaagggg ttgacgttcc atatcgcgcc ttggacgaag attttgtgtt cgtaagccgc
     6121 catcagcata cccaaattgt agggcgtgag gcggtcgagc aggatgctgt tgctggggcg
     6181 gttgccgggg aactctttgt gcggcgcgag gcgttcgcgt tccgcttcgg gtaaatctgc
     6241 caactcggcg cgggcttcgt ccaaggtttt gcccttcatc agggcttccg cttgggcaaa
     6301 ggcgttggca acggtaaaac ggctgcgtcc ttcctccacg ccctgcgccg tcatcgggac
     6361 gataaagtcg cagggaatca ggcgcgtgcc ttgatgaagc agttggaaat aggcgtgctg
     6421 gcagttgacc ccttcaccac cgaacacgat gccgcccgtt ttgcacacgg cgggactgcc
     6481 gtctgaagcg cgacttttgc ccaaactctc catatcgagc tggttcagcc acgccggcag
     6541 caggcgcagg ttgtggctgt acggaacggc ggtctgcccg tccgcgtgct ggaaattgtt
     6601 ataccacacg gcaatcagcg ccatcagcac gggaatgttg cggcgcggcg gcgtgtggaa
     6661 aaaatgcctg tccatcgcgt gcgcccccgc caacaactcg cggaaacgcg ccccgccgac
     6721 cgcaaccatc acgggcaaac cgacaggcga ccagacggaa tagcgtccgc ccacccagtc
     6781 gtacatcgca aacacacgtt ccgccgcaat accgaaactt tgcgctgcat ccgtatcggc
     6841 agacaccgcg caaaaatggc gcgccgtttc ctgttccgag aaccctgcac cgcgatacca
     6901 cgccttgact gcctgtgcat tgagcagggt ttcgggcgtt ttgaaggact tgctggcaac
     6961 gcaaaacact gtcgtttcgg ggttcagacg gcataaaacc gcatccaggc aggcaggatc
     7021 ggcgttggcg gcaaaatgga cggcgatatg ccgtctgaac ggctcaagtg cctgcacgca
     7081 cattgccggc ccgaggtcgg atccgcctat gccgatgtgg acaaaatccg taatccgttt
     7141 tccggttgtc ccctgatacg aaccgtcgtc caaactgtgt gcaaactcca acgcacgatt
     7201 taactcgcgg cggatttcgg gcaacacgtc cctgccgtcc gcataaacgg catccgcacc
     7261 gtcgggtagg cgcaaagccg tatgcagcgc ggcacgcccc tcgctgccgt tgactttcgc
     7321 acccgtccgc aaagcacgca ttttcccttc caaatccgcc gtctccgcaa gacggcagag
     7381 cagttgcagc gtatcttcgc ccaaacggtt tttgctgtaa tcgaacaaca tcccgtccaa
     7441 acgctcatgc atacgctcaa aacggtcggg ttcgcaggca aagcggtcgc gcaaaaggac
     7501 atgacgcgta tcctgataat ggcgttcgag cgcataccat gcacgggtaa aagcattcat
     7561 ctgttttcct tgatttttca gaaccggatt aaaatgtagc agaatgtagt ttaacaaacg
     7621 gcagcggctt tggcgaatct ccggaacacc gcccgccgcg tccaatagca ccttatttcc
     7681 atatggcgaa aatatagtg
//
LOCUS       DAWGJV010000097         7680 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWGJV010000097 DAWGJV010000000
VERSION     DAWGJV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.097"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(92..1096)
                     /gene="rfaD"
                     /locus_tag="WHQ94_001470"
     CDS             complement(92..1096)
                     /gene="rfaD"
                     /locus_tag="WHQ94_001470"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ2128730.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(1225..2256)
                     /locus_tag="WHQ94_001471"
     CDS             complement(1225..2256)
                     /locus_tag="WHQ94_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ2128731.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(2258..3493)
                     /gene="dcm"
                     /locus_tag="WHQ94_001472"
     CDS             complement(2258..3493)
                     /gene="dcm"
                     /locus_tag="WHQ94_001472"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2128732.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(3530..4501)
                     /gene="hldA"
                     /locus_tag="WHQ94_001473"
     CDS             complement(3530..4501)
                     /gene="hldA"
                     /locus_tag="WHQ94_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ2128733.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(4556..5296)
                     /gene="pyrF"
                     /locus_tag="WHQ94_001474"
     CDS             complement(4556..5296)
                     /gene="pyrF"
                     /locus_tag="WHQ94_001474"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ2128734.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(5822..6469)
                     /gene="adk"
                     /locus_tag="WHQ94_001475"
     CDS             complement(5822..6469)
                     /gene="adk"
                     /locus_tag="WHQ94_001475"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ2128735.1"
                     /translation="MKALFLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            6684..7523
                     /gene="htpX"
                     /locus_tag="WHQ94_001476"
     CDS             6684..7523
                     /gene="htpX"
                     /locus_tag="WHQ94_001476"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ2128736.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         1954 a         1826 c         1711 g         2189 t
ORIGIN      
        1 tccactataa aaaccgccct cgcatgaaat caaaaatgcc gtctgaaaag ttcagacggc
       61 attttctttc aaacaaaatt accaatcgtg tttaagccaa attttccagc atccatttga
      121 cgtagcggtc gacgcctgat ttgacatcga aaaattcttc cttatatccg gcttcgcgca
      181 gtttggtgat gtcggcttgg gtgaagctct ggtatttgcc tttgagcgcg tcggggaagg
      241 ggatataacg gataagttct tcttctacca actctttcaa gctcatttca ggtttgcctt
      301 cggcggcgcg gcaggcgttg acggtggcag cggcgagttc gttgaactgt tggctgcggc
      361 cggtgccgag gttgtagatg ccggaaagtt cgggatggtc gaagaagtag aggttgactt
      421 tggcaacgtc ttcgacgctg acaaagtcgc gggtttgttc gccgttgccg tagccgtcgt
      481 tgctgccgaa caggttgacg taaccgtgtt cgcggtattg gtggaaatgg tggaaggcga
      541 cggatgccat acggcctttg tgttgttcgt gttgtccgta cacgttgaag tagcggaagc
      601 cgacgacttg ggcggtcaag ccttctttca tgcggcggcg caatacttgg tcaaacagga
      661 atttggaata gccgtacaca ttgagcggtt tttcgagttc gcgctcttca cggaagattt
      721 cgcctttacc atacacggcg gcactggagg cataaaggaa gggaatgcgt tcgtcctgac
      781 accagtccaa caaatccagc gtgtactggt agttgttttc catcatatac aaaccgtcgt
      841 gattcatggt gtcggaacac gcgccttgat ggaaaacggc ttcgatgttt tgataaggta
      901 aaatgtgttc cctcacttgg cggatgaatt catgtttgtc gagataatgg gcgatttcgc
      961 actcggcaag gtttttgaat ttttcgcctt tgctcaaatt atcgacggca acaatgtcag
     1021 taataccgcg ttgattaagt gctttgacga tgttgctgcc gataaagccg gccgcgcctg
     1081 ttacgatgat ggtcatattc ggtttccttt gttttgagta aaattaataa acttagttac
     1141 aagaaatcat cgatattgtc ctgaaacaca aatttgcgat gatattccaa atatttttct
     1201 cttccttcgg gatatattct catcttaatt ttttgcattt ggattgatat taagtttttc
     1261 ccatgtgtaa cggcttagtc gagtaccgca aatcaaacga ccatcatcca agaaagtaat
     1321 ataaccttga tcaaacaacc aatcataagt cggcgttaaa gctaacccat ttaaataatc
     1381 taatgcctct ttctcatttt tctcagtaat acaaaccata taaggtttaa tatgactagc
     1441 ccttaacaaa atctcatctg taattaatgt aaatggacat tgcggcatat gatttataac
     1501 atccttgcga tatttctgct gcccttttct acttttctgt ttaatgattt cttgctctac
     1561 gaattgatta aattctgaag ccggtatttc taatttttct cttgataaga gttgcgggtg
     1621 cacgatagag cgaaattgat aatccaaaaa tattcggaaa taaaataatg gttctgaatc
     1681 ttctatatct ttgacaggca atagtttaag aattgataaa taactaattt tgggcaatac
     1741 aatcttacgc caaattgacc atatatcatc ctccgaacgg atataacccc tacggtaacc
     1801 tttagaattt tccaaatggt ctgatacatc atggattgaa aagaaaatat tttctgtagg
     1861 cagattatta atacttaaaa cgtaatcatc ataaaacctt gaaatatcat gatgatattt
     1921 ctgttcttgt ttgtgatatt caacttttgc atcaaccaaa taacgctgta aatttttctt
     1981 attaaaaaaa caatttttct gaatgaaagg atattctacc ctccttccat tttttgtctc
     2041 agtaatcccc caattttcat actcaaaaaa tgtacttagt tttcgaccac tatcatcaat
     2101 ataactacct acatatttgc gtgcttcccc attaccacca aaaacagata acttgttatt
     2161 cctgtgaata aaactatctt ccgcacgtaa attttgaata ctatcgacaa taaaatactc
     2221 tatgttttca atagtaattc tttgaaaatc cggcatttta ctctccataa agcgtaatat
     2281 ccatttgttt taatatagcg attaatacat caacaacaat actgtttccc gcttggcgat
     2341 acatttgagt atcactaact aaaattttaa aatcatctct aaaacccatt agccgcaaac
     2401 attccctagg tgttaattta cgaatacgtc cacgattatg agtaacataa ttatcaaccc
     2461 cggcacgatg cattttatgc atagtttgca ataatgggcg agctacaggc aaatcagttt
     2521 ccgtactggt tttaaaattt tttgttcctc ctgccaaaac ataatttttg attttttcag
     2581 aaagataata tttttcctca acgttattta catcaaaaat aaagtcatca aattcagatt
     2641 cgcgggctgc ctgaaaaata aaatcaccat gccaattaaa ttgttgattt gctttttggc
     2701 ataaggcaat ttctccatta atctgtgtat aacgtttttg tctattttta gaactggtaa
     2761 caaatttcac acccttttca cgtaaaaaat atttgctatc agtatagtcc tccaaaaaat
     2821 cttgcatagt atgttctaat tcaatctttt caggaaactg aaaaccattt ataggagggg
     2881 tatgaaagcc aacaacaaaa atacgctcac gatgttgagg aatcccataa tccttactat
     2941 tcattatttg gaaatataag tcataaccaa gtgaataaaa aacacttttt acaactttcc
     3001 aagtttttcc attatcatga ttgagcaagc cctttacatt ttcataaata aatatctttg
     3061 gttggacttc atccacaacg cgagcaaatt catagaataa tgttcctcgt gtatcttcta
     3121 atcctgcacg tttgccaacc atggaaaatg cttggcaagg actgcctcca actaaaatat
     3181 caacttgatt tctaaacttt ctcgcatcaa attgagtaat gtcgttatgc caaaaatctt
     3241 catttaattt atagtttcca agataacttt ttttaacgta tggatcaata tctcctgcaa
     3301 aaacaatggt atggtttaaa tttaatcggt ggaatgcctg ttcaaccgca ccaatcccgc
     3361 tgaatacggt tgctaatcta atatgtgaat caggtttaag aaaagtttta gatttccaac
     3421 cttgttgact gggaaagagc aaagtttttt gtaatcgagt atcgtgtgtc tgtgccattg
     3481 tcgaaatagt catacttata tcgttctgtt tatcttatca atatgaaaac tacattgttg
     3541 attgccctga caatgccttg atcaattcgg caaacgagca aaccgccgta ccgagtttcg
     3601 ccacgacaac cccggccgca gtattggcaa ggtacatggc ttcgggcatg gtgcagcctg
     3661 ccgccaaacc caagcccatt ccggcaatga cggtgtcgcc cgcaccggat acgtcgtaaa
     3721 cttcttgggc gcgggtgggc tggtaaatcg gttcgccttc gctgaacaag gtcatgcctt
     3781 cttcgcttcg ggtcagtaaa acggcggtca ggtcgaggtg gcggcgcagg ttttgcgctt
     3841 tttcggtcag ctcgctttcg tttttccaac tgccgaccac ttctttcaat tcggcgcggt
     3901 taggcgtaat cagagttgca ccgacatatt tttcgtaatc gtcgcctttg gggtcgatta
     3961 agacggtttt gccggcgtgt ttcgcccaat cgatcatatc ggagatatgc gacaggccgc
     4021 ctttgccgta gtctgaaaaa atgattgcgt cgtattcggg caagatttcg cggtatttcc
     4081 gcttgatttg ttccaacact tcgcggttag gatgttcttc aaaatcaagg cggataagct
     4141 gctggttgcg ggcgacgacg cgcagtttga cggtggtggc gatttgtttg tcgcgcatca
     4201 gataggaggc gacgccgtcc tgcaccatca gcgcatcgag cgcgtcggcg gcttcgtcgt
     4261 tgccggttac ggacaacagc cctgccctgc cgcccaacga agcgatgttg cgcgcaacgt
     4321 tcgccgctcc gcccgcgcgt tggtcgattc gtccgatttt cgccaccggc acgggggctt
     4381 cgggcgaaat acgggacaca tcgccgaacc aatagcggtc gagcatcacg tcgccgacaa
     4441 ccaggacttt ggcttgcgcg aaacgggatt tgagggtttc ttgttggaac ttggcggaca
     4501 tattgcctcc gttgtatttt ttcagacggc ctcagcctgt aaggccgtct gaaaatcagt
     4561 ttgcttcaag gtttgccacg cggttcactt cgcgcaatac ggctaccgga tcggcagctt
     4621 gggttacagg acgacccatt accaaataag tcgaagcggc agccaaggct tcggccggtg
     4681 tcatgatgcg gcgctggtca tcattattgc cggcaacgtc caagcggatg ccgggcgtga
     4741 ccaagacaaa atcttgtccc aactcgcggc gcagcggtgc ggcttcttgg gcggaacaga
     4801 ccacgccgtc caagcccgaa ctttgcgcca gtttcgccaa gcggatgact tgttcttcag
     4861 gggcggtgtt caaaccgatt tccgccaaat cgctttgttc catgctggtc aacacggtta
     4921 cgccgattaa gagcggcttc gtgccgtatc cggcaacggc ttctgctgcg gcttccatca
     4981 tacggcggcc gcccgatgcg tgcatatcga ccatccaaac gcccatatcg gcagcgactt
     5041 tgcaggcttg cgcgacggtg tggggaatat cgtggtattt caaatcgagg aaaagtttga
     5101 aaccttgatt gattaagctt tctgccaaat tgcgtcccgt cgcggtaaac agctctttgc
     5161 cgattttgat ttgacacaat gtcgggtcaa ggttgcggac gaatccgagc gtgtcttttt
     5221 cgttggaaaa atcaagggcg acgataacgg gggtgcgttg ttgcggagtt tggaagtcgg
     5281 agattaaagg attcatggtg ttttcgtttc gggttttcag acgaccttta tcgtcttttt
     5341 cgcagtatag tggattaact ttaaaccggt acggcgttgt ctcgccttag ttcaaagaga
     5401 acgcttctct aaggtgctga agcacaaagt gaatcggttc cgtactgttt gtactgcctg
     5461 cggcttcgtc gccttgtcct gatttttgtt aatccactat aagaacgtaa attcaaaccg
     5521 taggtcgggc attgatgtcc gacctacgcg gactcaagcc tccgacaaac tgtttcggat
     5581 aaatttgaac cggtacatca gccgtcattc ccgcgtaggc gggaatccat tgataaaact
     5641 cagttcatca gatttaaaca gcgattgccc aaattcagaa atggattccc gcctgcgcgg
     5701 gaatgacgcc aaatgattat ttttcggttg caacacgctg tttatcaaaa acaggtcgtc
     5761 tgaaaacctg attttctgtt ttcagagtgc gaagcctgcc cgtgggtacg accttttcga
     5821 tttatttgcc caatgcgccc aatacttcgg ctttcacggc ttctaccggc tgagtgccgt
     5881 caactttgat gtatttaggc gcgtgttcgc cttccagttt gctgtaaaaa tcgaccaaaa
     5941 cttcggtttg ctcgtggtaa acggcaaggc gttttttcac ggtttcttct ttgtcgtcgt
     6001 cgcgctgaat caaatcttcg ccggttacgt cgtctttgcc ttcaactttg ggcgggttgt
     6061 aggtaacgtg gtaagtacgg ccggaagcca aatgcacgcg gcggccgctc atgcggtcga
     6121 caatcacgct gtcaggcaca tcgatttcaa cgactgcatc caaatccacg cctgcttcaa
     6181 ccatcgcttc ggcttgtgcc aatgtgcgcg ggaaaccgtc aaacaggaaa ccgtttttgc
     6241 agtcgtcttg cgcgatgcgt tctttgacca tgccgataat gatgtcgtcg cgcaccaagc
     6301 cgccttcatc aatgattttt ttcgcttcca aacccaaggg cgtgcctgcc ttaatcgcgg
     6361 cgcggagcat gtcgccggta gagatttgcg gaatgccgaa cgcggcggtg atgaattgcg
     6421 cctgagtgcc tttgcccgcg cccggcgcgc ctaaaaataa tgctttcatg aagtgatcct
     6481 ttgaaatgtg tttattggaa tcaaacagat ggtttacgat tctacacggc ttggcacaat
     6541 agttcaaaaa ccaactgcac aaactgccat ttgcaagcaa atgttttgca tacctttgcc
     6601 gagtctgata ttcacaagcc ggcaactttt tgggacaatc cccaaaattt caaaacatca
     6661 caccctacca aaaaggaaca tccgtgaaac gaatttttct gtttttggct accaatatcg
     6721 ctgttttggt cgtaatcaac attgttttgg cggttttggg catcaacagc cggggcggca
     6781 cgggcagcct gttggcgtat tccgccgtcg tcggcttcac tggttcgatt atttcgctgc
     6841 tgatgtccaa attcatcgcc aaacaatcgg tcggcgcgga agttatcgac acgccgcgca
     6901 ccgaagaaga agcctggctt ttgaacactg tcgaagccca agcgcggcaa tggaacctga
     6961 aaacgcccga agtcgccatc taccactccc ccgaacccaa tgcctttgcc acgggcgcat
     7021 cgagaaacag ctccctgatc gccgtcagca ccggtttgct cgaccatatg acgcgcgacg
     7081 aagtggaagc cgtattggcg cacgaaatgg cacacgtcgg caacggcgat atggttacgc
     7141 tgacgctgat tcaaggcgtg gtcaatacct tcgtcgtgtt cctgtcgcgc attattgcca
     7201 acctgattgc ccgaaacaac gacggcagcc agtcgcaggg aacttatttc ctagtcagca
     7261 tggtattcca aatcctgttc ggcttccttg ccagcttaat tgtcatgtgg ttcagccgac
     7321 aacgcgaata ccgcgccgac gcgggcgcgg caaaactggt cggcgcgccg aaaatgattt
     7381 ccgccctgca aaggcttaaa ggcaacccgg tcgatttgcc cgaagaaatg aacgcaatgg
     7441 gcatcgccgg agatacgcgc gactccctgc tcagcaccca cccttcgctg gacaaccgaa
     7501 tcgcccgcct caaatcgctt taaaccgatt tgaaacggca aaaaccgtac gcgcaagcag
     7561 tacggttttt tgtatatgct gtctgaaaac atcaggtttc cataccgaca ccaaccgtat
     7621 ccatttccgc accggatacg ccgcattgat ataccctgat aaaccgtatc cggacagaaa
//
LOCUS       DAWGJV010000098         7667 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWGJV010000098 DAWGJV010000000
VERSION     DAWGJV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.098"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(32..448)
                     /gene="dksA"
                     /locus_tag="WHQ94_001477"
     CDS             complement(32..448)
                     /gene="dksA"
                     /locus_tag="WHQ94_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ2128737.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(595..1386)
                     /locus_tag="WHQ94_001478"
     CDS             complement(595..1386)
                     /locus_tag="WHQ94_001478"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ2128738.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNICTNGALVLSVAAGLSVGTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1435..1843)
                     /locus_tag="WHQ94_001479"
                     /pseudo
     CDS             complement(1435..1843)
                     /locus_tag="WHQ94_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(1843..2529)
                     /locus_tag="WHQ94_001480"
     CDS             complement(1843..2529)
                     /locus_tag="WHQ94_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ2128739.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2649..3692
                     /locus_tag="WHQ94_001481"
     CDS             2649..3692
                     /locus_tag="WHQ94_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ2128740.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3855..5081
                     /locus_tag="WHQ94_001482"
     CDS             3855..5081
                     /locus_tag="WHQ94_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ2128741.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5367..7517
                     /gene="yccS"
                     /locus_tag="WHQ94_001483"
     CDS             5367..7517
                     /gene="yccS"
                     /locus_tag="WHQ94_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ2128742.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1879 a         2556 c         1675 g         1557 t
ORIGIN      
        1 ggggcttcat acggcatttt gtttgccgcc atcagtcggc aaactgtttt ttcatcctct
       61 ctcggcgttc ttgggactca acagataaag tggctgtcgg gcgtgccagc agccgcttca
      121 gaccgatagg ctctcccgta tcggcacaga atccataatc cccttcatca atattgcgga
      181 tggtcgcctg tattttactg agaagttttc gttcccgatc gcgggtacgg agttccaatg
      241 cgtactcttc ttcctgtgtg gcacggtcgg caggatcggg ggctgattcg tgttcttgga
      301 gatgccctgt cgtagcggaa gcattttcga tgagttcgtc ttgcattttt accagcaatt
      361 cgcggaaaaa agccaaatgg tcgtcattca tataatcgtc ttccggcccg ctccaattca
      421 aaatatcttg ttctgtcagc tttgccataa tgtatatcct ctcggttcac cgaaaaaaac
      481 aaagcaccac aaagtggctt tattcctttg atttttataa ggcttattgc ctaaggcgtt
      541 tttgaaaacg cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg
      601 atattgccgt tccatttcct gcgaacggcg cacacaggca caaacgccct cgcttatggc
      661 ttcggcgaca cgatgccgcc tgaaagcttc cacggcttcg tgggttgtcc cgccttttga
      721 cgttacattt ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggtaaccgc
      781 tcctttaaac gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat
      841 ggcggcattt tgcaatgcgt ccagcagata aaacacataa gccggtccgc tgccgctgat
      901 accggtaatg ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt
      961 catgattcga tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg catacatacc
     1021 ggatacgccc agtcctattt tcccgggcgt attcggcata acccggacaa tgcggcggct
     1081 tcccccgagg taacggctga gcgtaccgac tgacaatccg gctgcgacag aaagcaccaa
     1141 tgcgccgttg gtgcagatat ttttgcacgc ggcttccata tcctgcggtt tgacggcaag
     1201 gattaaaaca tcgtcggaat gaagctccgg cagggttgcc gaagtttcga cccccaactc
     1261 tttttccaaa cgttcgcgtt tttccgcacc ccgattggct atatagatgc ggtaaccgcc
     1321 ttgtttgacc aatccgcccg caacggcagc cgccatattg ccgccgccga gaaaataaac
     1381 attcattatt tctaccctat tcaaaattac ctgactaaat tcattcccaa ttttttacca
     1441 tcggacgcgc tcggacagac cgttgcgccg caacccctgc ttttccaagc agtcaaatcc
     1501 tccctgtttg aacgtctcaa tcaaaggttt gccgtcacgc gtacgccaat ccagcccgca
     1561 ggcttcaaac ttccctattt tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt
     1621 tgccctgacg gaacgattgc cgtctgaaag cggtatctcg tagtcctcta aagacggcgg
     1681 actctcgccg ccaatatccc aaaaatcgat ggcagccggt tttctcggct tacaccagcc
     1741 ggacagggac gaacactcaa taccaacccg ccaaccggca gcctgcaacc gtaaatgcaa
     1801 gcaacgcagc cattgccgta tattttattg cccgaatttc catcagcccc ttttcccgaa
     1861 aatcgcgctg ccgatacgga cgtgtgtcgc accgcactca atggcggcag gcatatcgtc
     1921 cgacatcccc atagacagca cgtccgcctt aacgccagcc gtattgaggt cggcaagcaa
     1981 cttttgcatg gtctgaaact gggttttcaa ctccgtttca ctgctgttgg ctttggcaac
     2041 gcacatcagt ccgcgcacga caaggttggg caacttcgcc acttcgaccg caagcgcgac
     2101 cgcttcttcg ggagcaacac cgtgctttgc cgcctcgcct gcaatattca cctcgataca
     2161 cacctgcaaa ggcggcattg aagaaggacg ctgcccactc agccggacgg cggtctttag
     2221 acggcatacg gtatgcaccc aatgcgcgcg ttcggcgaca aacttggttt tgttggactg
     2281 cacatcgccg atgacgtgcc acacgatgtc ggtcaaatcc gccaactctt ccgttttgcc
     2341 gtaccactcc tgaatatagt tctcgccgaa atcacgctgt ccggcggcgt aaacttcgcg
     2401 gatgccgtct gaagggaaag tcttaccgac ggcaatcagg ctgacggaat gcggcttcct
     2461 tcccgcctgc agaaccaatt ttccaatacg gtcggacacc tcccgataac gttcttgcaa
     2521 caccgtcata gattatcccc taattaaaaa tgattaaaca gttgaaaccc ttccagtcag
     2581 ggcggtacaa tcaaggttgt tagaaccatt ccaaccaatc gaaacattat actaaacaga
     2641 gccgcattat gcagattacc gacttactcg ccttcggcgc taaaaacaaa gcatccgacc
     2701 ttcacctgag ttcgggcata tcccctatga ttcgggttca cggcgatatg cggcgcatca
     2761 accttcccga aatgagcgcg gaagaggtcg gtaatatggt aacttcggtg atgaacgacc
     2821 accagcggaa aatctaccag caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg
     2881 tcgcccgatt ccgcgtcaac gccttcaaca ccggccgcgg tcccgccgcc gtattccgca
     2941 ccattcccag caccgtctta tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa
     3001 tcgcagaatc gccgcgcggc atggtattgg ttaccggccc taccggttcg ggcaaatcga
     3061 ccacgcttgc cgcgatgatc aactacatca acgaaaccca gccggcacac atcctgacca
     3121 tcgaagaccc gatcgaattc gtccaccaaa gcaaaaaatc cctgattaac cagcgcgagc
     3181 tgcaccagca caccctcagc ttcgccaacg cgctgcgttc cgcattgcgc gaagaccccg
     3241 acgttatcct tgtcggcgag atgcgcgacc ccgaaaccat cggcttggca ctgaccgccg
     3301 ccgaaaccgg acacttggtt ttcggcacgc tgcacacgac cggcgcagca aaaaccgtcg
     3361 accgtatcgt ggacgtattc ccggcgggag aaaaagaaat ggtgcgctct atgctgtccg
     3421 aatcgctgac cgccgtcatc tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg
     3481 tcgcctcgca cgaaatcctg attgccaacc ccgccgtccg caacctcatc cgcgaaaaca
     3541 aaatcacgca gattaactcc gtcctgcaaa ccgggcaggc gagcggtatg cagacaatgg
     3601 accaatcgct gcaatcgctg gtgcgccaag ggctgatcgc accggaagtc gcacgcagac
     3661 gcgcgcaaaa cagcgaaagt atgagtttct gacacacaac cgccttccgg ccataccggc
     3721 gggaaaacaa ggcgcaaaca cgcggggcgg gacgcagcat cccgcccggc tacctttccg
     3781 aacaagaagc gtccgccttc ctgttgaaac ctgccgccgc aaactgcaag gcttaaaccg
     3841 aaaagaagct aacgatgaat accgataacc tgcacgacat cttggacgaa atggttcaag
     3901 tgtattctca aaaaaaacaa agccgatccg aaaccccggc cgaaatcggc gcacacttcc
     3961 acccgctgct cgaccgcctg tgcgaaaccg cagaagcaca aaacgcgtcc gacatcctta
     4021 tcagcaaagg attcccgccc tcgttgaaaa tcaacagcgc attaaccccg cagccgcaaa
     4081 aggcgctgac aggcgaggaa accgccgcca tcgccgcatc gacgatgaac gccgaacaat
     4141 cggaaatatt ccggcgcgac ggcgaaatca actactccgt ccagtcgcgc agcggcacgc
     4201 gctaccgcgc caacgcctac cacagccaag gcagcgcagg tttggttttg cggcgcatca
     4261 accacgtcat cccgcaaatg caggaattgg gcctgcccga aaaactcaaa gacctcgccg
     4321 tcgcaccgcg cgggctgctg attatcgtcg ggcctaccgg ttcgggcaaa tccaccacga
     4381 tggcgactat gctcgaacac cgcaacaaaa ccctgcccag ccatatcgtt accatcgaag
     4441 acccgattga gtttatctac aaaccgcgcc gctgcatctt tacccagcgc gaaatcggcg
     4501 tcgacaccat aaactggcag acggcggtac aaaacgctat gcgccaatcc cccgacgtgg
     4561 tctgcatcgg cgaagtccgc agcagggaaa gtatggaata cgcgatgcag ctcgcccaaa
     4621 ccggccacct gtgcattttt acgctccacg ccaacaccgc gccgcagtcg ctcgaacgca
     4681 tactcaactt ctaccccaaa gaacagcaca accaaatact gatcgacatc gccctcaacc
     4741 tgaccggcat catctgccaa cgcctcgccc tcaaacaaga caaaacgggc aggacggcgg
     4801 ttgtcgactt gctcatcaac acgcccgcca tccaagactt catcctgaag ggcgacctga
     4861 tgaacatcag taaaatcatg gaaaccgcca aaaccgacgg aatgcagacg atggatcaaa
     4921 accttttcga actgtaccgt cacggcatca tcagttacga agaagccctg cgccagtccg
     4981 tttccgccaa caacctgcga ttgcacatcc aactgcacaa agaaggcaaa acgcccgaac
     5041 tcctttacga cagggtcaac ggtctcaacc tcatttcctg atccgcaaaa cccaatgccg
     5101 tctgaaaacc gcatccccgt tttcagacgg catgatttta tccgccccat tcatgtgcta
     5161 cactttatag tggattaaat ttatagtgga ttaacaaaaa tcaggacaag acgacgaagc
     5221 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     5281 gttctctttc agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatattca
     5341 cttcataaca ataaaccggt aaaaccatga aaaccccact cctcaagcct ctgctcatta
     5401 cctcgcttcc cgttttcgcc agtgttttta ccgccgcctc catcgtctgg cagctaggcg
     5461 aacccaagct cgccatgccc ttcgtactcg gcatcatcgc cggcggcctt gtcgatttgg
     5521 acaaccgcct gaccggacgg ctgaaaaaca tcatcaccac cgtcgccctg ttcaccctct
     5581 cctcgctcac ggcacaaagt accctcggca cagggctgcc tttcatcctc gccatgaccc
     5641 tgatgacctt cggctttacc attttaggcg cggtcgggct gaaataccgc accttcgcct
     5701 tcggcgcact cgccgtcgcc acctacacca cacttaccta cacccccgaa acctactggc
     5761 tgaccaaccc cttcatgatt ttatgcggca ccgtactgta cagcaccgcc atcatcctgt
     5821 tccaaatcat cctgccccac cgccccgtcc aagaaagcgt cgccaacgcc tacgacgcac
     5881 tcggtggcta cctcgaagcc aaagccgact tcttcgaccc cgatgaggca gcctggatag
     5941 gcaaccgcca catcgacctc gccatgagca acaccggcgt catcaccgcc ttcaaccaat
     6001 gccgttccgc cctgttttac cgccttcgcg gcaaacaccg ccacccgcgc accgccaaaa
     6061 tgctgcgcta ctactttgcc gcccaagaca tacacgaacg catcagctcc gcccacgtcg
     6121 attatcagga aatgtccgaa aaattcaaaa acaccgacat catcttccgc atccaccgcc
     6181 tgctcgaaat gcagggacaa gcctgccgca acaccgccca agccctgcgc gcaagcaaag
     6241 actacgttta cagcaaacgc ctcggccgcg ccatcgaagg ctgccgccag tcgctgcgcc
     6301 tcctttcaga cagcaacgac aatcccgaca tccgccacct gcgccgcctt ctcgacaacc
     6361 tcggcagcgt cgaccagcag ttccgccaac tccgacacag cgactccccc gccgaaaacg
     6421 accgcatggg cgacacccgc atcgccgccc tcgaaaccgg cagcctcaaa aacacctggc
     6481 aggcaatccg tccgcagcta aacctcgaat caggcgtatt ccgccatgcc gtccgcctgt
     6541 cccttgtcgt tgccgccgcc tgcaccatcg tcgaagccct caacctcaac ctcggctact
     6601 ggatactact gaccgccctt ttcgtctgcc aacccaacta caccgccacc aaaagccgcg
     6661 tccgccagcg catcgccggc accgtactcg gcgtaatcgt cggctcgctc gtcccctact
     6721 ttaccccctc cgtcgaaacc aagctttgga tcgtcatcgt cagtaccacc ctctttttca
     6781 tgacccgcac ctacaaatac agtttctcca ccttcttcat taccattcaa gccctgacca
     6841 gcctctccct cgcaggtttg gacgtatacg ccgccatgcc cgtacgcatc atcgacacca
     6901 ttatcggcgc atcccttgcc tgggcggcgg tcagctacct atggccagac tggaaatacc
     6961 tcacgctcga acgcaccgcc gcccttgccg tatgcagcaa cggtgcctat ctcgaaaaaa
     7021 tcaccgaacg cctcaaaagc ggcgaaaccg gcgacgacgt cgaataccgc gccacccgcc
     7081 gccgcgccca cgaacacacc gccgccctca gcagtaccct ttccgacatg agcagcgaac
     7141 ccgcaaaatt cgccgacagc ctgcaacccg gctttaccct gctcaaaacc ggctacgccc
     7201 tgaccggcta catctccgcc ctcggcgcat accgcagcga aatgcacgaa gaatgcagcc
     7261 ccgactttac cgcacagttc cacctcgccg ccgaacacac cgcccacatc ttccaacacc
     7321 tgcccgaaac cgaacccgac gactttcaga cagcactgga tacactgcgc ggcgaactcg
     7381 acaccctccg cacccacagc agcggaacac aaagccacat cctcctccaa cagctccaac
     7441 tcatcgcccg gcagctcgaa ccctactacc gcgcctaccg acaaattccg cacaggcagc
     7501 cccaaaacgc agcctgaaaa agtttcggca ttttgtaaga gagggcagat tgtcagacag
     7561 gttgcaagat agtggataaa gctttgcgca gggtaaatgc gtagcaactg aaccgtcatt
     7621 cccacgaacc tacatcccgt cattcccacg aaaacagaaa accaaaa
//
LOCUS       DAWGJV010000099         7533 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWGJV010000099 DAWGJV010000000
VERSION     DAWGJV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.099"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            16..420
                     /locus_tag="WHQ94_001484"
     CDS             16..420
                     /locus_tag="WHQ94_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ2128743.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WHQ94_001485"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WHQ94_001485"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ2128744.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="WHQ94_001486"
     CDS             1376..1723
                     /locus_tag="WHQ94_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ2128745.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WHQ94_001487"
     CDS             1728..2321
                     /locus_tag="WHQ94_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ2128746.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WHQ94_001488"
     CDS             2385..2993
                     /locus_tag="WHQ94_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ2128747.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3633
                     /locus_tag="WHQ94_001489"
     CDS             3001..3633
                     /locus_tag="WHQ94_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128748.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3673..4452
                     /gene="map"
                     /locus_tag="WHQ94_001490"
     CDS             3673..4452
                     /gene="map"
                     /locus_tag="WHQ94_001490"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ2128749.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4607..4906
                     /locus_tag="WHQ94_001491"
     CDS             4607..4906
                     /locus_tag="WHQ94_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ2128750.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5144..5518
                     /locus_tag="WHQ94_001492"
     CDS             5144..5518
                     /locus_tag="WHQ94_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ2128751.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5704..7170)
                     /locus_tag="WHQ94_001493"
     CDS             complement(5704..7170)
                     /locus_tag="WHQ94_001493"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ2128752.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1940 a         2267 c         1847 g         1479 t
ORIGIN      
        1 gaagaggcgg aatgggtggg cagcctgttc ggcctgcaca aatctttcgg tttccttacg
       61 ctgacggtga ttacattgcg catcgtgtgg gcggttgcca accgcgccaa gcgtccgcaa
      121 agcgacagca aggctgcggc ggcaggacac ggcattctgt atctgctcat gcttgccgtt
      181 cccgttatcg gcatgatccg ccaatacggc agcggccgcg gcccgttgaa agtgttcggc
      241 gttgaggtga tgcagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctg gctgctgttt gccgccgtcg tcggacacgt cgccatggtc
      361 gtcgtccacc gtgttcaagg caaagacgtg ctgtaccgca tgacggggcg tgtccgttga
      421 ttccgttcgc actatggtgc cggtccgtcc ggcatcattt gtttttccaa gacagtgcca
      481 gatcgtacaa agcttttttt ccctcgcccg tgattttggc ggcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacaattcc accatatcgg
      781 caagcgtcgc cccgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacaatttc ctacgttcgc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tcccgccaca ccagccacac tcaaagccgc catcaccgcg cttgcgccga
      961 caacagggac aactttaaac ccgacctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacgctg acgagttggc
     1141 cctgaatgcc gtacgcgctc aaaagctgcg cggtaacgcg cgtatcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcctgcggc aacggcggtt cggctgccga cgcgcaacac ttcgccgccg
     1921 aaatgaccgg ccgttttgaa aaagaacgca tggaactcgc cgctgtcgcg ctgacaacag
     1981 acacttccgc gctgacagcc atcggcaacg actacggttt cgaccacgta ttcagcaaac
     2041 aggtgcgcgc gctcggacgt gcaggcgatg tattggtcgg catttccacc tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgcct
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttgctca
     2221 acgttcccca tccgcgcacc gcccgtattc aagaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagca cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtatta
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcggca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacgc ccaaagaaaa ccataccaaa ccggacaccc cgaaaaacac gccgcccaaa
     3601 ccccataaag aaattctcga caacctcttc tgacccggca cggcaggcac acccgcaatc
     3661 caaggaagca ttatgaacgg catcatcatc aaaacccccg aagaaatcga aaaaatgcgc
     3721 gagctgggca aactcgtcgc cgaagccctc gactacatcg gacaattcgt caaacccggc
     3781 gtaaccaccg acgaaatcga caaactcgtt tacgactacc acgtcaacgt ccaaggcggc
     3841 tatcccgccc ccctgcacta cggcaacccg ccctacccca aatcctgctg cacctccgtc
     3901 aaccacgtca tctgccacgg cattcccgac gacaagccgc tcaaagaagg cgacattatc
     3961 aacatcgacc tcaccatcaa aaaagacggc ttccacggcg actccagccg tatgtttacc
     4021 gtcggcaaag tctcccccat cgcccaacgg ctgatcgacg taacccacgc ctccatgatg
     4081 gcgggcatag aagccgtcaa acccggcgcg acactgggcg acgtaggcta cgcctgccaa
     4141 caggttgccg aaaacgccgg ctattccgtc gtacaggaat tctgcggaca cggcatcggg
     4201 cgcggtttcc acgaagcccc gcaagtgttg cactacggaa aaaaagggca gggccccgtt
     4261 ctaaaaccgg gtatgatttt taccgtcgaa ccgatgatca accaaggcaa acgccacctg
     4321 cgtatcctca acgacggctg gacggtggtt accaaagacc gctccctctc cgcccaatgg
     4381 gaacacgaag tcttggtgac cgaaaccggc tacgaaatcc tcaccgtcag ccccgcctcc
     4441 ggcaaaccct gaagccggac gtatccgccc cataaaaaca aacaatgccg tctgaaagaa
     4501 acggcagata tgatatataa tataaaaaca ggcttgaccc ggcacattac gaaaacaaag
     4561 caaatcggaa tttgccccgc aaccagacaa acttaaagga agttttatga aaatatttga
     4621 aaatatagaa gatgttaaag ccatccgtaa aaagaccggg ctgaaccaga tagacttctg
     4681 gggcaaggtc ggcgttaccc agtctggagg ctcgcgctac gaaaccggcc gcaaaatgcc
     4741 caaacccgta cgcgaactgc tccgcctcgt ccatatcgaa tgcatcgatt tggcgaaagt
     4801 caacaaaaaa gatatggaaa tcgccgccct gttgaaaaaa caccatcccg acctgtatgc
     4861 cgaattgtcc aaacagacca agtccgaaag aaaaaaacaa agttaaaccg caacctccgg
     4921 atgcccgaaa gtttttcatt tccgaaaaac gcaaacaatg ccgtctgaaa caccggacag
     4981 gtcgccgtat cccgcctgcc gcccctgcct caaaccgccg aaccgcccga acccgccttt
     5041 ttacaaactt tatccaattt cctgtttatt tcaggatacg ccgacattag aatgtcaaac
     5101 agctcgaaac gggcaaactc cacatccatc caaaggaata aaaatgaaac ttctgaccac
     5161 cgcaatcctg tcttccgcaa tcgcgctcag cagtatggct gctgctgccg gcacgaacaa
     5221 ccccaccgtt gccaaaaaaa ccgtcagcta cgtctgccag caaggtaaaa aagtcaaagt
     5281 aacctacggc ttcaacaaac agggcctgac cacatacgct tccgccgtca tcaacggcaa
     5341 acgcgtgcaa atgcctgtca atttggacaa atccgacaat gtggaaacat tctacggcaa
     5401 agaaggcggt tatgttttgg gtaccggcgt gatggatggt aaatcctacc gcaaacagcc
     5461 cattatgatt accgcacctg acaaccaaat cgtcttcaaa gactgttccc cacgttaatc
     5521 aggcaacaaa aaacagcgtt ttcagaaatg aaaacgctgt tttttgactg ttccattatt
     5581 cacaaaaggg aaaaacgatt acctgccccg tgtaccaaaa cctgccctgc cggatgaagg
     5641 gcataactgg cagggacggc gtcaacacca tatgggggta cggcttttct tgaaagattc
     5701 ggcttaaata tccaatactt tcgcggtata ggcgataatt tcatccgccc tttcagggtt
     5761 ttcgttcaac ttgatgccgt aacccggtac cagctctttc agacggtctt cccaagacgg
     5821 ggcgcgctcg gggaagcatt ggtgcatcag ccggatcatc agcggcacag cggtcgatgc
     5881 gcccggcgac gcgcccagca atgcggcgag tgagccgtcg gcgtgggcga caatctccgt
     5941 accaaactgg agcacgccgc ctttttcgga gtctttttta atgatttgga cgcgttgccc
     6001 tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg tagtattcca gcagggaggc
     6061 gaagcgttct tctttggttt tacgcaattc gcccagcagg tatttggtca gcggcatatt
     6121 cgcccagccg gcgcacagca taggatagag gttgtccata tggatggaca gcggcaaatc
     6181 cataagcgag ccttgcttga ggaagttgga acggaagcct gcgtaagggc cgaacataag
     6241 gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg acggacatcg gcggcgcgcc
     6301 gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg gcggtttcgg ggttgctgtt
     6361 gcggaagaac aggccggaca cggggaagcc gccgtagcct ttgccttcgg ggatgccgga
     6421 tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg aagaggaagc gggtacggag
     6481 ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg agcacccacg cgccgtcgga
     6541 ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt tttacgccct tgccctgcaa
     6601 atatttcacc atttggcgcg tcagccgtcc gaaatcgaca tccgtacctt cggcggagta
     6661 gttggcggcg acgggttggt tttcgtcccg gccgcgcatc atcagcggag cccaatcgga
     6721 aattttgttc cgatcggtgg aaaattccat attttcaaaa agtttttggg ttttaaacgc
     6781 gtcataacgt ttttgaagat aagaacaatg gtcttcattc atcaccaaag acatatgcgg
     6841 cacggcattg atgaaggaat tgtcttccaa cttgccttcc gcgaccagcg tcgcccaaaa
     6901 ctggcggctg acatgaaact gttcggcaat attgagggcg cgcgccggat cgataatccc
     6961 atttgcaccc aacggcgcat agttcaattc gcacagcgcg gaatgccccg tgccggcgtt
     7021 gttccacgcg tttgacgatt ccaacgccac atcttccaag cgttcaatca gggtgatttc
     7081 ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc gcgctcataa tgccgccgcc
     7141 caccaagaca acgtctgtcg cttcagccat ggtttactcc taaaaaacag gcatcttctg
     7201 cccttatggt tatttgccgt actacaaacg cctgaatcgc aaaagcaggg aaaaccgaca
     7261 atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc gttgcaagta tggcttccga
     7321 caccgcttca ggggcttgta atatgttatc gtgaatgtag tggattttac tgggaaatgc
     7381 aaagtttttc tgtcgcccgc caagtcggga aactgcgaaa tgaaaaataa aaatagttat
     7441 ttatctatat atatcaaatt tttaatagat aaaaaatcaa aattgtttat atattaattt
     7501 ttaaaagatt gtcagcatat tgcgttaagt ttt
//
LOCUS       DAWGJV010000100         7505 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWGJV010000100 DAWGJV010000000
VERSION     DAWGJV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.100"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(15..953)
                     /locus_tag="WHQ94_001494"
     CDS             complement(15..953)
                     /locus_tag="WHQ94_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128753.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            1156..2268
                     /gene="nadA"
                     /locus_tag="WHQ94_001495"
     CDS             1156..2268
                     /gene="nadA"
                     /locus_tag="WHQ94_001495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ2128754.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKTEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            2425..2754
                     /locus_tag="WHQ94_001496"
     CDS             2425..2754
                     /locus_tag="WHQ94_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ2128755.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIISIGLILLGVVLLNT
                     MSHMSGH"
     gene            2804..4312
                     /gene="nadB"
                     /locus_tag="WHQ94_001497"
     CDS             2804..4312
                     /gene="nadB"
                     /locus_tag="WHQ94_001497"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ2128756.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(4488..5153)
                     /gene="pgmB"
                     /locus_tag="WHQ94_001498"
     CDS             complement(4488..5153)
                     /gene="pgmB"
                     /locus_tag="WHQ94_001498"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ2128757.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(5166..7424)
                     /locus_tag="WHQ94_001499"
     CDS             complement(5166..7424)
                     /locus_tag="WHQ94_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ2128758.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVTDFSVELDMQHGVLCRSFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKVLGGSQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKTAGRAL
                     SEKIAGVAFETLLEAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHAILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         1550 a         2063 c         2191 g         1701 t
ORIGIN      
        1 cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat cagcctgtcg
       61 ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct gcccgatacg
      121 ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa gttttgcttg
      181 tgcagcaatc tgccgctgat ggcttcgacg ctgttttgca gttgcagcag cgtgaattcg
      241 ggcggcatca gttcaaaaat cacggggcgg tatttgattt tggcgcgcag gcgggacagg
      301 gcggtcgcca gtacgcggcg gtggtcgtgg cgcatgggct gccccgtaag cgcgaagtcg
      361 aagtttgcct gcggctcggc ggcttccact atcagaccgc tttcatacag catttcatag
      421 cgttgtaaaa cgtattcttc cgaccagttt tccggttcga ccccccagca caaatgaatg
      481 cgcttgagcc gcatttgacg cacttcctcc gtgtccgccg aatttgccca aatgcgcagg
      541 cggctgacga cggcgtcgcg ctgcccgccg tcggtgcgca agtcttccca cgggaaatag
      601 ccgtagcagt cctgccattt cgcatccggg tgcaggatgc tgtcggctgc ctcgcgcacc
      661 agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg gtgggtatcg
      721 acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt ggcaacccac
      781 agcttcacgc ccgcctgcag ggaattgcgc agaggggaga gcgggccgtt gggcaggagt
      841 gtgccttggg cgacggttaa tacccgcagg ccgccgtcgg taacggcaat caatacggga
      901 accagctcga cgatgctttg cggcggagct tcggcttcgg ggtaggcatc catgctcggc
      961 tttctgcggg tggaaaaggc tttattgtaa accaaagcgg tttgtcggaa cgaagcgggt
     1021 cggttttgcg gtatcgcaaa cctgttttca gacgaccttg catattatac tcaagttgag
     1081 cataatgtaa aaacagtttg aaaagcagac ggcaaacctt catgccgtct gaaaaaccag
     1141 ctacaaggaa acatcatgca aaccgccgcc cgccgctcgt tcgattatga tatgccactc
     1201 atccaaacgc cgacttctgc ctgccaaatc cgtcaggcgt gggcaaaggt tgccgacacg
     1261 cccgaccgcg aaacggcagg tcgtctgaaa gacgaaatca aggccttgct gaaggagaaa
     1321 aatgcggtct tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggaa
     1381 acgggcggat gtgtgggaga ttcgctggaa atggcacgct tcggcgcgga acacgaagcc
     1441 ggtacattgg tggtggcggg tgtgcgattc atgggcgaga gcgcgaaaat cctctgccct
     1501 gaaaaaacgg tgctgatgcc tgatttggaa gcggaatgtt ctttggattt gggctgtccg
     1561 gaagaggcgt tttcggcgtt ttgcgaccaa caccccgacc gcacggtggt ggtgtacgcc
     1621 aacacttccg ccgccgtgaa agcgcgtgcc gattgggtgg taacgtcttc ggtggcgttg
     1681 gaaatcgtgt cgtatctgaa atcgcgcggc gagaaactga tttggggacc cgaccgccac
     1741 ctcggcgact acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc
     1801 atcgtccaca acgaattcaa agggcaggag ctggcggcgt tgaagacgga acaccccgac
     1861 gcggtggtgc tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc
     1921 ggctcgacca gcaaactgct caaagccgcc gtatcgcgtc ctgaaaaaaa attcatcgtg
     1981 gcgaccgatt tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa acaatttatc
     2041 gccgcgccga cggcgggcaa cggtggaagc tgcaaaagct gcgcgttctg cccgtggatg
     2101 gcgatgaatt cgctgggcgg catcaaatac gccctgacaa gcggacgcaa cgaaatcctg
     2161 ttggacagga agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgactttgcg
     2221 gcagggctga agaaaaaaga tgtgttcaac ggcatgggcc ccgcctgatt tgtcgttcgt
     2281 aatgggtgca gcctgaaaat accgcccgcc tgctttccca agacggattt gcacacaaaa
     2341 aatctttaac aacaagactg tttttttatt ttattgcatt gatataatta tactttcttt
     2401 catttatcag gtaaaaacat gcaaatgcac tggctctttc tgactgtagc aattttaagc
     2461 gaagtctgcg gttcttccat gctcaaactg agtggcgggt ttagcaaact gtggccttct
     2521 attggcgtgg tagtcagctt ttcggtgtgt ttttgggcct tgtctatgac actgaaaacc
     2581 atgccgctgg ctacagcata cgccatttgg gcaggcgtgg gactggtttt aacggcttta
     2641 gtcagcgtgg tgtttttcgg tgagaaagct gatttcattg ggattatcag catcggtttg
     2701 attttgctgg gcgtggtgct gctcaatacc atgtcgcata tgtcggggca ttgatttaat
     2761 ccactatacc gtttcagacg acctctccaa caaggacaac accatgcaaa ccgattgcga
     2821 cgtattgatt gccggaaacg ggctggcggc actgacgctc gccctgtcgc tgcctgaatc
     2881 gttccgcatc gtcattttgt gcaaaaaccg gctggacgac accgccagcc gtcatgcgca
     2941 aggcgggatt gcggcggcgt ggtcgggaga ggacgacatc gaaaaacacg tcgccgatac
     3001 tttggaagcg agcgcaggtt tgtgcgacga agccgccgtc cgcaccatcc tgtcgcaggg
     3061 caaaccggca atcgaatggc tgctggcgca gggcgtggcg ttcgaccgaa attatgacgg
     3121 cctgcacctg acgcgcgaag gcgggcacac ctgccgccga atcgcccacg tcgccgacta
     3181 cacgggcgaa gccgtcatgc agagcctgct tgcccaaata cgccgccgcc cgcacatccg
     3241 cgtttgcgag cggcagatgg cgttggacat tcaaaccgaa tcaggtgtgg cgtgcggact
     3301 gatcgtcctt gaccgccgaa cgcaagaaac ctacctcatc cgcgcccgcc ataccgtact
     3361 cgcaggcggc ggcttgggac agatttacgc cgccaccacc acgccgcccg aatgcacggg
     3421 cgacgccatc gccatggcga tacgcgcagg ctgtgcagtt gagaacctcg aatttatcca
     3481 attccacccc acaggcttgg caagatcgtc tgaaaacggc cgtaccttcc tgatttccga
     3541 agccgtgcgc ggcgaaggcg gcatcctgac caaccaagcg ggcgaacggt ttatgccgca
     3601 ttacgaccgc cgcgccgaac tcgcgccgcg cgacatcgtt gcccgcgcca tcgccgccga
     3661 aatcgccaaa caaactccag acttcgtctc gctcgacatc agccgccaac ccgaagcgtt
     3721 cgtccgccgg cattttccgt ccatccatcg gcactgttta tcccaatgcg gcctggacat
     3781 cacgcgccaa gccatccccg tccgccccgt gcaacactac acctgcggcg gcatccaaac
     3841 cgacccctgc ggcagaacct ccctgccgca gctctacgcc ttaggcgaaa ccgcctgcac
     3901 cggcttgcac ggagccaacc gcctcgccag caactccctg ctcgaatgtg tcgttaccgc
     3961 cagacttgcc gcccaaacca tcgcggacgg acaagcattc caagccaaaa cgtctccaag
     4021 gccgtctgaa aacccctccg ccgaagcagg catctttgca gacgaccttc aaaacacatt
     4081 cagccgcccc gtcctgcaaa cgttcaacca acgccatctc ggcatcctac gcagcgatac
     4141 cggcctgcgc cgcgccatcg cccaactgcg gctttggaag caaaaccaag ccgaaccgca
     4201 caccgcgtcc gaatacgaaa accgcaacct gctcgaatgc agcctcgccg tcgcccaagc
     4261 cgcatacagg cggagacaga acatcggcgc gcattttaat agtgattgtt aaggttggct
     4321 ggttagaaaa aatcgttcaa atgaatttta atagcatggt taagagattg gcattcagtt
     4381 tttagggttg cgtaagttgg gttatcaatc tgttctaaaa gcagaaaggc cgtctgaaac
     4441 actgttcaga cggcctttta cttccttgac actttatctg acgcgtttta cctgcccgac
     4501 tgtgcccaca cgttttgcag gtaggcgtag gtcagctcgg cggtatcgga caccagcgcg
     4561 atgtcgctgc ccaagtcttc ggctttgccc acgccgatag gcaaggcgcc ggcggctttg
     4621 atggcggcga cacctgcggc agcgtcttca atgccgatgc agcggcggat gtccgcgccc
     4681 acgccctcgg cggcggcgag gaagatgtcg ggggcgggtt tggaatgcgc gacggcggca
     4741 gggtcggcga cggtgtcgaa gaagtgggtc agccccatgc gttcgagcag gaaggggccg
     4801 tttttactgg cggacgcaag ggcgattttt ttgccgtttg cttttaatgc ttccagcagc
     4861 ggcaaaatgc cgagatacac gtcttcgggt ttgactgcct gaatcatctc gacgtagttg
     4921 tcgtttttgc ggcgggtcag ttcggcgaac tcggcttcgc cgacggtttt gccgccgtgt
     4981 gcgaggatgc gtttgagcga atcgtcgcgc gacacgcctt tgagctgctc gttaaacttg
     5041 cggtctatgc cgatgcccag ttcttcggcg agctttttcc atgcgcggta gtggtattcg
     5101 gcggtgtcgg tgatgacgcc gtcgagatcg aagagcactg cggtaaacgt cattttgcgc
     5161 cctccttatt tttccaacgc ggcggtatgg ctgccgttga gtgtgatgtc tttgccgtac
     5221 acctgcaaat cgagcggctc gcctttgagc agggtgaaga cgacgttttc tttgccgacg
     5281 gcgactttaa tcagacggcc tcggtagttg atatggaagg cgtagcctgt ccatgcgctc
     5341 ggcaggaacg gtgcaaagct gagtttgccg ccccaagttt tcatttgggc gaaaccttgg
     5401 acgatggcga gccacgagcc ggtcatagaa gtaatgtgca ggccgtcttc tgtgtcgttg
     5461 ttgtagttgt ccaagtccag gcgggcggtg cgccggtaca tttccacggc tttttcttct
     5521 ttgcccagtt cggcggcaag gatggcgtgg atacacggcg acaacgagct ttcatgcacg
     5581 gtcatcggtt cgtagaagtc gaagttgcgg cgtttttcgt cgatattgaa acggtcgccg
     5641 aagaagtaga tgccttgcaa gacgtccgcc tgtttgatga agggcgaacg caggattttg
     5701 tcccacgacc atttctggtt gagcggcaaa tcgtcgggcg aaagcgcgga cacggggcga
     5761 atgtctttat cgaggaagcc gtcgtgctgc acgaatacgc cgagttcttc gtcatgcgga
     5821 cggtacatat tcgcgctgat gtccgcccat ttttccaact cggcggcacg cacgttcaaa
     5881 tccggacgcg ggtatttcgc caaggcttcg cgggtgtagt ccaataccca tgcggcgagg
     5941 gtgttggtgt accagttgtt gttgatgttg ttttcgtatt cgttcggacc ggttacgccg
     6001 tgaatcatgt atttgccgtt gcgtttggag aagtggacgc ggtccgccca gaagcgggac
     6061 acttcgacca agacttccaa gccttctttg gcaagatagc tttcgtcgcc ggtgtagttg
     6121 gtgtagttat agatggcgta aggaatcgcg ccgttgcggt ggatttcctc gaaggtgatt
     6181 tcccattcgt tgtggcactc gatgcccgta aacgttacca tcggatagag tgcgcccgcc
     6241 aagccctgtt cgcgcgcgtt gtgttgtgcc tgcggcagtt ggttgcggcg gtattgcagc
     6301 aggttgcggg taacttcggg ttccgccagt gcgaggtaaa gcggcacggc gtaggcttcg
     6361 gtgtcccaat aggtcgcgcc gccgtatttt tcgccggtaa agcctttcgg gccgatgttc
     6421 agtcgcgcgt cttcgccgta gtaggtggag aacagttgga acaggttgaa gcggatgccc
     6481 tgctgtgctt cgtcgctgcc ttcgatgacc acgtcggcga tttcccaacg gtgcagccag
     6541 cctgctttgt gcgcctccag caaggtttca aacgcaacgc ctgcgatttt ttccgacaag
     6601 gcgcggccgg cggttttcac tgcttccaag ccctgataat cgcggctggt ggtaacgatc
     6661 acgcgttttt caaaggtttc gggcgtgccg ccgacttcgg cctcgaaaga attagaaact
     6721 tgccaatcgg tttggctgcc gcctaagact ttgaagctgc cggcgaaggt ttgctcggcg
     6781 ttgacgataa attgttccac accgaaaggg ttggcaacgg tttgggcggc aatgtaggag
     6841 agactgtctg aaacgccttt gtccaatacc tgccagaatt tttcttcgta gttggagtct
     6901 tcgtttttca cgtcggcatc gatgatggaa tcgatgcgga cttggtgggt tttgccgtca
     6961 acggatacgg cttcccagcg gataaccgcc agctcttttt gcgcgacgga caggaatttg
     7021 cacacatcga aacgcacgcc gaatacggtg aacgagcggc acaacacacc gtgctgcata
     7081 tcgagttcga cggagaagtc ggtaacgtcg tttttcgcca agtccacctc ttgtccatcg
     7141 acaaagattt taactttgct gaaattgagc gcgttgatgg ctttgccgaa atatttggga
     7201 tagccgtttt tccaccagcc gacgcgggtt ttgtcgggga accacacgcc ggcgatgtag
     7261 gtgcccaagt ggctgtcggc ggagtaggtt tcctcaaagc tgccgcgcat acccatatag
     7321 ccgttgccca aactggtcag gctctcttgc agccgtttgt gttctttttc cagctttgcc
     7381 gaacgcagcg tccaagggct gatttccata attcttgtgt acatttatga agctcctgtt
     7441 tggattgatt tgagggaatg gtgaaatctt atagtggatt aacaaaaatc aggacaaggc
     7501 gacga
//
LOCUS       DAWGJV010000101         7483 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWGJV010000101 DAWGJV010000000
VERSION     DAWGJV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.101"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..270)
                     /locus_tag="WHQ94_001500"
     CDS             complement(<1..270)
                     /locus_tag="WHQ94_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ2128759.1"
                     /translation="MTGFNDAAGVASTASDIKGKYVQSVTVANGVVTAQMASSGVNKE
                     IQGRKLSLWAKRQNGSVKWFCGQPVERNAKATADAVTAATPDTDKI"
     gene            complement(316..684)
                     /locus_tag="WHQ94_001501"
     CDS             complement(316..684)
                     /locus_tag="WHQ94_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ2128760.1"
                     /translation="MASSNVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRAAPAGTNN
                     DEVKAATGTNDKIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISRYRVLPESRQMAE
                     KLRHSRKSGNLGLSAQKLIG"
     gene            complement(713..1042)
                     /locus_tag="WHQ94_001502"
     CDS             complement(713..1042)
                     /locus_tag="WHQ94_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2128761.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1120..1338)
                     /locus_tag="WHQ94_001503"
     CDS             complement(1120..1338)
                     /locus_tag="WHQ94_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ2128762.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            1460..2413
                     /locus_tag="WHQ94_001504"
     CDS             1460..2413
                     /locus_tag="WHQ94_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ2128763.1"
                     /translation="MNHKKIVVLDADTLPGRIFHFDFPHELAVYGTTGADETAERVHD
                     AHVVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2491..4548)
                     /gene="metG"
                     /locus_tag="WHQ94_001505"
     CDS             complement(2491..4548)
                     /gene="metG"
                     /locus_tag="WHQ94_001505"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ2128764.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTATN
                     AARFLNLDAITWTNTRVTLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4664..6502)
                     /gene="glmS"
                     /locus_tag="WHQ94_001506"
     CDS             complement(4664..6502)
                     /gene="glmS"
                     /locus_tag="WHQ94_001506"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ2128765.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATKGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6781..7299
                     /locus_tag="WHQ94_001507"
     CDS             6781..7299
                     /locus_tag="WHQ94_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128766.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPTLLF"
BASE COUNT         1545 a         1778 c         2256 g         1904 t
ORIGIN      
        1 gattttgtcg gtgtcgggtg tggcggcggt gacggcgtcg gcggtggctt tggcgttgcg
       61 ctcaaccggc tgtccgcaga accattttac cgaaccgttt tgacgcttgg cccacaggga
      121 gagttttctg ccttggattt ctttgtttac gccgcttgaa gccatttggg cggtaacgac
      181 gccgtttgcg actgtaacgc tttgaacata tttgcctttg atgtcggagg cggtggatgc
      241 cacgccggcg gcatcattaa atcccgtcat tcccacgaaa gtgggaatct aggactcagg
      301 actggagaaa ccgttttacc cgataagttt ctgcgccgac aaacctagat tcccgctttt
      361 gcgggaatga cgaagttttt ctgccatttg ccgtgattcg ggcaatactc ggtaacggct
      421 gattttttgg aagtgtttga agtcggcgcg tggtgtttct atgcaaccgg tagatgaatc
      481 atcgcggcag gttgacggca ggtgctttgt gtcgattttg tcgttggtgc cggtggcggc
      541 tttgacttcg tcgttgttgg tgccggcggg ggcggcgcgc gtaaccggct gtccgcagaa
      601 ccattttacc gaaccgtctt gacgcttggc ccacagggag agttttttgt ctttgatttc
      661 gttgtttacg ttgcttgaag ccattatgtc agacggtatt gcccgggcag ctttattcgt
      721 acactttcag cagctcgact tcaaatatca aagtggcgtg cgggggaatc acgccgcccg
      781 cgccgtgtgc gccgtagccc atttccgaag ggatggtcag cttgcgtttg ccgccttcct
      841 tcattccgcc gaagccttcg tcccagcctt tgatgacttg tccgacaccg agcgtgatgg
      901 tcagcggctg gcggcggtcg aggctggagt cgaatttggt tccgttttcc agccaacctg
      961 tgtaatgcac ggtaatctct ttgcctttaa ctgcttcttt tccgaagcct tcttgcaagt
     1021 cttcaataat caggccgccc atatttgtcc tttcattgct tgttggtcaa aacggcaagg
     1081 gtaacatacc gtccgtcgaa gtcaaatgcc gctcaaacgt cagctgcatc ggtgcagctg
     1141 aaacggctgt gtttgttgga ctgttttatt tttttcgtaa aggttccatg ctttttcatg
     1201 gaaatagaaa acgacggtgt tgattagggg ctcgaccagc gcgaccgccc ccgatacgcc
     1261 tatactgccc gtcagtacat aggttacact gaaggcggtg ctgaaatgca gtgcggcaaa
     1321 agtcagggtt ttaagcatca tcctctctcg gattggacat tgacggagga atgataaaga
     1381 ttatcataag gatgtgcggt ttaaatttgc tatttactgt tagtgtaaat aaatcaactt
     1441 tcaaataagg atagaaataa tgaatcacaa aaagatcgtt gttttggatg cggatacttt
     1501 gcccggccgg atttttcatt ttgattttcc gcacgagctt gcggtttacg gtacgacagg
     1561 tgcggatgaa acggcagaac gggtgcacga cgcacatgtt gtcattacca ataaggtaat
     1621 gatttccgcc gacattattg cggctaatcc gcagttggag ctgattgccg tcagtgcgac
     1681 cggtgtgaac aatgtcgata ttggggcggc gaaggcggcg ggcgttgcgg tatgcaatgt
     1741 ccgcgcctac ggaaacgaat cggttgcgga acacgccttt atgctgatga ttgcgttgat
     1801 gcggaatttg cctgcctatc agcgtgatgt tgcggcagga ttatgggaga agtcgccgtt
     1861 tttctgccat tacggcgcgc cgattcggga tttgaacggt aaaacgctgg cggttttcgg
     1921 acgcggcaat atcggacgga cgcttgccgg atacgcgcag gcattcggta tgggggtggt
     1981 gtttgccgaa cacaaacacg cgcccgatgt tcgcgaaggc tatgtttcct ttgaagatgc
     2041 ggtacagact gccgacgtgt tgtcgctgca ctgtccgctg aacgcccaaa ctgaaaatat
     2101 gataggcgaa aacgaattgc ggcagatgaa gcccggtgcg gttttgatta attgcgggcg
     2161 cggcgggctg gtggatgaaa acgcgctgct tgccgcactc aaatacgggc agatcggcgg
     2221 ggcgggtgtc gatgttttga cggaggagcc gccaaggggc ggcaatccct tgctgaatgc
     2281 acgcctgccc aatctgattg ttacgccgca taccgcgtgg gcgagccgcg aggctttgga
     2341 caggctgttc gatatattgt tggcgaacat tcacgccttt gtgaaaggcg aggcacaaaa
     2401 tcgggtggtt tgagcttgtc gggattatgg gaaaacaatg ccgtctgaag gcctcaaggg
     2461 tccagacggc attgtttttg atatttctac ttaaccgact ttgtcgcccg gttgcgcgcc
     2521 tgcgtcgaca tcaagcagtt tcagtttccc gtctgccgtg gcggcactca aaatcatacc
     2581 ttcggatacg ccgaattttg ccattttgcg cggggcgaag ttggcgacgg cgatgaccat
     2641 tcttccgttt aactcggcag ggttgggata agacgcggcg ataccggaga agatgatgcg
     2701 tttttcaaaa ccgaaatcga ggtcgaattt caaaagtttg gtgctgccct cgacggcttc
     2761 gcagttcaag actttggcga cgcgcatatc aattttcata aagtcgtcga agctggcctg
     2821 ttcggcgact ttttcgtatt tgctctcttc ggcggcaggt gcggctgcgg cggcgatgct
     2881 ttgtttgttg gcttcgatta aatcgtccac ttgtttttgc tccactcgtt gcattaaatg
     2941 tttgtatttg ttgatggcgt gtttgcccaa ggttaccctt gtgttcgtcc aagtaatcgc
     3001 gtccagattg aggaaacgcg cggcattggt ggcggtttgc ggcaatacgg gggcgaggta
     3061 ggcggtcagc atcgtaaagg cgttgatgag ttcgctgcat acttcgtgca ggcgttcgtc
     3121 ttggccttct tgtttggcga gttcccaagg tttgttggca tcaacgtatt cattaacaat
     3181 gtctgccaat gccatgatgt cgcgcagggc gcgggcgtat tcgcggtttt cgtagcattc
     3241 ggcaatggct tcgctttgcg cagtcagttt tgccagcaat gcgctgtcgg aaacgtcttt
     3301 gagttttcct tcaaagcgtt tggcgatgaa acctgacgcg cgggcggcga tgttgacgta
     3361 tttgccgacg aggtcgctgt tgacgcgcga gataaagtct tgcaggttca aatcgatgtc
     3421 ttcgattttg ctgttgagtt tggcggcgat gtagtagcgc atccactcgg ggtttaggcc
     3481 ttgttccaga taggatttgg cggtaataaa cgtgccgcgc gatttggaca ttttttgtcc
     3541 gtccacggtc aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc cggagaaatg
     3601 cagcatggcg ggccagaaca gggcgtggaa atagaggatg tctttgccga tgaagtggta
     3661 catctcggtt tggctgccgg ctttgaagta ttcgtcgaaa tcgatgccga tgcggtcgca
     3721 caggttttta aacgatgcca tgtagccgac gggcgcgtcc agccagacgt agaagtattt
     3781 gcccggcgcg tcggggattt caaaaccgaa atacggcgcg tcgcgggaaa tgtcccagtc
     3841 ggacagggtg gtttcttcgc cttcgcccag ccattctttc attttgttga gggcttctgg
     3901 ttgcagatgg ggtttgccgt cgtgcgggtt gttgccggaa gtccatgctt tgaggaagtc
     3961 ggcgcattcg cccagtttga agaagaagtg ttcggattcg cgcaattcgg gtttcgcgcc
     4021 ggaaacggcg gaatacgggt taatcagttc ggtcggggaa taggtcgtgc cgcagacttc
     4081 gcagttgtcg ccgtattggt cttgggcgtg gcatttcggg cattcgcctt taacgaagcg
     4141 gtcgggcagg aacatttgtt tttcggggtc gaaaagctgc tcgatgacgc ggctctcaat
     4201 cttgccgttg gctttcagcg cgcggtaaat atcttgggaa aactgtttgt tttcagggga
     4261 atgggtgctg taataattgt cgtagccgat gttgaaaccg gtaaagtcgg cgaggtgttc
     4321 ttcgcgcact ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg cggcaagcat
     4381 cacgggcgtg ccgtgggtgt cgtcggcgca gcagtagtag cattcgttgc cgcgcagttt
     4441 ttggaagcgc acccaaacgt cggtttggat gtgttcgacc atgtggccga ggtggatgct
     4501 gccgttggca tagggcaggg cggaggtaac taagattttg cgtgtcatat tgtgctttgc
     4561 aaacaatggg taaaggcgga ttataccgca aatcaaacgg ggaaatgccg tctgaagcct
     4621 gaaaaatcgg gcttcagacg gcatttttgc aaaacggcgg gagttattcg acggttacgg
     4681 atttcgccag gttgcgcggc ttgtccacat cggtaccgcg tgcgagggcg gtgtggtagg
     4741 cgaggagctg cacggggata gtatgcacga cgggggacag tttgccgacg tggcgcggtg
     4801 cgcggataac gtgcacacct ttggtggcat taaaattact gtcgaggtcg gcaaagacga
     4861 aaagttcgcc gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc
     4921 tgtcgttggg tgcgatgacg acgacgggca tgttttcgtc caccaaggca agcggcccgt
     4981 gcttcagttc gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagtttta
     5041 acgcgccttc gagggcaatc gggtaatgga tgccgcgccc caaaaacagc gcgctggttt
     5101 tcttggcaaa ctgttgcgcc caggcggcaa tttgcggttc gaggtttaaa gcgtgctgca
     5161 cgctgccggg aagctggcgg agttcttcgg tgtaacgtgc ttcgtcttct tcggaaacca
     5221 aaccgcgcac tttcgccagc gttaccgcca aaccgaacag cgcaaccagc tgcgtggtaa
     5281 atgctttggt tgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc
     5341 tttcgcgcgg cagggcggat tccatcacgt tgcagatgga gaggctgtgg cggtgtccca
     5401 aggattttgc gtatttcaac gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg
     5461 taatgaccag ttggtccgga tcggcaatca cgctgcggta tcggtattcg ctggcgattt
     5521 cgacatcggc cgggattttg gcgatggatt ccaaccaata tttggcggtc agcgcggcgt
     5581 aataggacgt gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg
     5641 cgtttttgcc gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc gccgtgtctg
     5701 caatcgcgcg cggctgctca tggatttcct tttgcataaa gtggctgtac ggtcccagtt
     5761 ccaaagaggc gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgcttt
     5821 tatcggtcag ccgtttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcgaggt
     5881 acgccacgcg gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat
     5941 cgtcgcccaa agcgaccaaa agcgggcagc ccatacgcgc cacaactaat tcatcaggct
     6001 tgtcttgggc aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcttgta
     6061 ccgcttcaaa cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgtgcgatga
     6121 cttcggtatc cgtttgcgat tcaaaacggt atcccaaacc ttccaagcgt ttgcgttcgc
     6181 tttcaaagtt ttcgatgatg ccgttgtgga cgaccgcaat catcccgccg ctgatgtgcg
     6241 ggtgggcgtt cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccga
     6301 tgccgccgct gatgcctttt tcgcgtgccg cgtcctccat aagctgcacg cgtccgacgc
     6361 ggcgcacgcg tttgattttg ccgtcggtgt tgacggcaat gcctgatgag tcataacccc
     6421 ggtattcgag gcgtttgaga ccgtcggtca gaaaatcgac gacgttgtga tgggcgcgga
     6481 tggcgccgac gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaacaggcgc
     6541 ggacggctcc cgtgccgcac cttcctcttc ggattataaa ccgcctcccg cgccgaaaaa
     6601 cagcaaaatg ccgcctgaag gcttgggctt gctcaaaaaa gggagggatt tccccgcctg
     6661 accgggatgg gcgttcagac ggcattgcct gccgccggtt tatagttttt acaaatcaat
     6721 attgacaagc tgaaaaaaaa caatatactc gctcggtctt aatgttaacg gagtatggaa
     6781 atgaaacaaa tgcttttagc cgtcggcgtg gtggcggtgt tggcgggctg cggcaaggat
     6841 gccggcggtt acgagggtta ttggcgcgaa aaatcggaca aaaaagaggg cgtgattgcc
     6901 gtcaaaaaag aaaaaggcaa ttacttcctt aataaaatca acgtatttac aggcaaggag
     6961 gagtctttgc ttttgtctga aaaagacggc gcgctttcga taaacacggg gataggggaa
     7021 atcccgatca aactttccga tgacgggaaa gagctgtatg tcgaacgcag gcagtatgtc
     7081 aaaaccgatg cggcgatgaa ggacaaaatc atcgcccatc agaaaaagtg cggacaaacg
     7141 gcacaggcat acctcgacgc gcgaaaggcg ttgccgtcaa accaaacgta tcagcagcgt
     7201 caggcggcga tcgagcaatt aaaacggcgg tttgaagccg agtttgacga attggaaaaa
     7261 gaaatcaaat gcaacggcag accgacattg ttgttttagc gtaggacaac agggaggatg
     7321 ccgccgtccg aatcggacgt gcggtttccg taccggtacg ggcgggcagg aatgtccgcc
     7381 tttttttgtt cggacgcgtt tgaatacccg tttgattccg accgtttgca aggggtattt
     7441 ccgttcgggc ggaaattata gtggattaac aaaaaccagt acg
//
LOCUS       DAWGJV010000102         7481 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWGJV010000102 DAWGJV010000000
VERSION     DAWGJV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.102"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1..162)
                     /locus_tag="WHQ94_001508"
                     /pseudo
     CDS             complement(1..162)
                     /locus_tag="WHQ94_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(468..1727)
                     /gene="rho"
                     /locus_tag="WHQ94_001509"
     CDS             complement(468..1727)
                     /gene="rho"
                     /locus_tag="WHQ94_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ2128767.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1947..4331)
                     /gene="ppsA"
                     /locus_tag="WHQ94_001510"
     CDS             complement(1947..4331)
                     /gene="ppsA"
                     /locus_tag="WHQ94_001510"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ2128768.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4742..5563
                     /locus_tag="WHQ94_001511"
     CDS             4742..5563
                     /locus_tag="WHQ94_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ2128769.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5803..6465)
                     /locus_tag="WHQ94_001512"
     CDS             complement(5803..6465)
                     /locus_tag="WHQ94_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2128770.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6503..7330)
                     /locus_tag="WHQ94_001513"
     CDS             complement(6503..7330)
                     /locus_tag="WHQ94_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ2128771.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT         1568 a         1793 c         2190 g         1930 t
ORIGIN      
        1 ggactgtttt gataaataag taatcgtaaa cgatgaaaat aataggctgc ggtactttta
       61 attaacgcct actaactctg ctgctgttct tgcagttaca cctgcgacaa atagctcaat
      121 gagtttattt tgtttatacc ggcttagacg actttttctc ataaaggcaa ctctaactta
      181 atttgaattt ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc
      241 ggcgcaacct tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag
      301 ccggctttgc aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc
      361 gttaacgggg ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc
      421 gattttcagg cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc
      481 atcagctcga agaaatcgtc gttgttttta gaggctttga tttttccgat taaaaattcg
      541 gctgcctcga tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgcaac
      601 tggtcgttcg gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg
      661 gggaagaggc gtttttccgc catacggcgg tcgaggtgca attccatatt gccggtgcct
      721 ttgaattctt cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgctgtggca
      781 atgatggtca gcgaaccgcc ttcttccacg ttgcgcgccg cgccgaagaa acgtttggga
      841 cggtgcagtg cgttggcatc gacaccgccg gttaggattt tgcccgaggt aggcacgacg
      901 gtattgtagg cgcgggcaag gcgggtaatc gaatcaagca ggatgaccac gtcttttttg
      961 tgttccacca tacgcttggc tttttcaagt accatttcgg caacttggac gtggcgttgc
     1021 gccggctcgt caaatgttga agaaaccact tcacctcgga cggaacggct catttcggtt
     1081 acttcttcgg gacgctcgtc aatcaagagg acgatgagtt cgacttcggg atagtttgcg
     1141 gtaacggcgt gggcaatgtt ttgcagcatc acggttttac cgcttttggg cggggcaacc
     1201 aagagggcgc gctgaccttt gccgatgggg gaaatcaggt cgatggcgcg tccggtcagg
     1261 ttttcttcgg actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc
     1321 aggttttcaa acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggtatca
     1381 agcctgacca gggcaaaata gcgttcgttg tcttttggga cgcgcacgct tccttcgatg
     1441 gtgtcgcccg tatgcaggtt gaagcggcgg atttgggtgg gcgagacata gatgtcgtcg
     1501 gggccggcaa gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt
     1561 tcaagcgtgc cggagcaggt gaaaccctcg ccttttttca tcatctggcg gacgatggca
     1621 aatacgaggt cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat
     1681 tctaagagtt tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat
     1741 tttgaagagg aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt
     1801 gctcggggaa gggggaattg taggcagtcg gcgagtgggt gtcaaatatt atcgcggacg
     1861 gggcatcggc aggaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt
     1921 ttcaggctcg ggggtatggg cattgattat ttgttcaatt cattcgccaa atatagccaa
     1981 gtttcgatga cggtatccgg gttcagggaa acgctttcga taccttcctc aaccagccat
     2041 ttggcgaagt ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc
     2101 ttgcggcagg cggagatggc aaggtgcagc atcactttga cggcagggtt gcgttcgtca
     2161 aacgattcgg ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg
     2221 ttcgagccga tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg
     2281 ctcggcagct cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt
     2341 tctttcaggg ctttgacaac ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg
     2401 atttcaacgt tggtcagacc catttcatcg cggacgcgtt tcaaggcttt gcattccaag
     2461 gcgaaacagt ctttgaagtt gtcggcgaca taacgcgccg cgccccggaa gcccaacatc
     2521 gggttttctt catgcggttc gtatacgttg ccgccgacca ggttggcgta ttcgttggat
     2581 ttgaagtcgg acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc
     2641 acgccttcgg cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg
     2701 cgggtaattt ccgcttttaa ttcgtcgtct tgtttgtcaa attccaacaa ggctttcggg
     2761 tggataccga tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg
     2821 ggcaggttgg cgaagctgaa tgcgagttcg gggttgccga cgttcatcat gactttgacg
     2881 ggtgctttgg gcatattgtc caaggcgaca tcggtgatct gtacgtccaa caggccggca
     2941 tagatgaagc cggtatcgcc ttcggcgcag gatacggtca cttcctgacc gttttccaag
     3001 agttcggtcg cattgccgca gccgacgacg gcaggaatac ccagttcgcg cgcgatgatg
     3061 gcggcgtggc aggtacggcc gccgcggttg gtcacgatgg cggaagcacg tttcatcacc
     3121 ggttcccaat ccgggtcggt catatcggta acgagtacgt cgccggcttc gacggaatcc
     3181 atctcggaag catctttaat caggcgtacc ttgccctgac cgactttctg accgatggca
     3241 cggccttcgc ataatacggt tttatcgccg ttgatggcga agcggcgcag gttgcggctg
     3301 ccttcttcct gggatttgac ggtttcgggg cgggcttgca ggatgtagag tttgccgtcc
     3361 aagccgtcgc gtccccactc gatgtccatc gggcggccgt agtgtttttc gatggtcagt
     3421 gcgtaatgcg ccaactcggt gatttcttcg tcggtaatgg agaagcggtt gcggtcttct
     3481 tcggggacat cgacgttggt taccgatttg ccggcttcgg ctttgtcggt aaaaatcatt
     3541 ttgatgtgtt tcgaacccat ggttttacgc aggatggcgg gcttgcccgc tttgagcgtg
     3601 ggtttgaaca cataaaattc gtccgggttg accgcacctt gtacgacgtt ttcgcccaga
     3661 ccgtaagagg aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac
     3721 atcacacctg atgcgccgct gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg
     3781 acgatatcgt gttcgaagcc tttgtggaca cggtaggaaa tggcgcggtc gttgtacagg
     3841 gaggcgaata catggtgcat cgcttcttta acgttttcca atccgttgat gttcaagaag
     3901 gtttcctgtt ggccggcaaa tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt
     3961 acggcaacgg aaatgtccgc gccgccggca tcggcaacca ttttgttcca tgccgcttcg
     4021 atttcggcat cgagctgttc ggggaagggt gtgtccaaaa tccattggcg gatttctttg
     4081 ccgacgcgtg ccagttcggc aacgtcttcg acatccaatt ttgccagtgc ggcggaaatg
     4141 cgttcgctca gaccgttgtg tgcgaggaat gcgcggtagg cttcggctgt ggtggcaaag
     4201 ccgccgggga cgcgaacgcc tttttcggtc agctgactga tcatttcgcc cagcgaggcg
     4261 tttttaccgc ccacgcgttc aacatctgtc atacgcaggt tttcaaacca aattacgtag
     4321 ttgtcggcca tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct
     4381 tattttaagc gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc
     4441 cgtctgaaaa attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatactt
     4501 ctttttttgg gtattttctt tgtaaaacag gtggtttgaa taggttaatg ttttttctgt
     4561 ttgatttttt tgtttatttt ttaaaatttt ctgccaaaaa atactttata taaataattt
     4621 tatttcaaaa ttatattgtg tctgtttggg tgtaatccga ggtaggcgtg ctgcgggtgc
     4681 tttccttgtg tctgccgctg ctgttatgat gatattttta ttctgtattt aaggatggaa
     4741 gatgagcagt ccgcgccatg tgttttacat ttccgaccgt accggtctga ctgccgagaa
     4801 tatcggcgag gctttgctga accagtttgg caatctgtcg ttcaaacgcc atacgcatcc
     4861 gtttgtcgat acgccggaaa aggcgcgcgc ggtggtggag aaggtcaatc ggagccggca
     4921 ggaaaacggt cagcgtccga ttgcgtttgt cagtgtggtt gatgacgaaa tccgtcggat
     4981 tatcaaaggg gcggatgctt ttcagattaa tttctttgag acttttttgg gactgttgga
     5041 gaaagaactc aataccgaag ccacggcatc cgagcagggg catcacagta tcggtaatac
     5101 gaagcgttac gatgcgcgta tggaggcggt aaatttctca ttgaaccacg atgacggggt
     5161 cagcgataag aaccttcagg aagcggatgt aatcttgatg ggtgtatcgc gttcgggcaa
     5221 aacgccgacc tgcctttacc tcgccctgca atacggcatc cgtgcggcaa actatccgct
     5281 gattcctgac gatttggaat cggccgatct gccgcgtatg gtcaagcctt atagggataa
     5341 gctgttcggg ttgaccatcc agccggaacg tttgcaggcc atccgccaag agcgccgccc
     5401 gaattcaact tatgccaaaa tcgatacatg ccgcagcgag gtggcagacg cgcagagtat
     5461 gttcagacgg catgggattc cgtttgcgaa tacgacggat aaatcagtgg aagagcttgc
     5521 cgtacacatc cttcaggcgt gcaagctcaa acgcaggttt tgacgggttt tgattcggtt
     5581 tgaaggtgga aatgccgtct gaaatcaggt ttcagacggc agttttatag tggattaaca
     5641 aaaatcatga caaggcgacg aagccgcaga cagtacaaat agtacggagc cgattcactt
     5701 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgctgtactg
     5761 gtttagattt aatccactat atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     5821 cagacattcg agcagatcga gcggcgttcg gatgtggaaa tccgcctgcc atgagtcggt
     5881 atcgtcttca tctgagatgt agccccattc ggcgaggacg gttttcatgc cggcgttgcg
     5941 gcctgcttgg atgtcgcgtt cggcatcacc gacgtagagt gtgtgttgcg ggtcggcgtg
     6001 gatttgtccg cacgcataca gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc
     6061 gccgctgacg acgacggcgg gtgggatgat gaagccgagt ttggggacga gtttgtcggt
     6121 gaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgttggt tgagttcggc
     6181 aatgagttcg ttcacgccgt caaagagctc ggtgtcttgg gcgtagcggc ggtcgtattc
     6241 ctccagatat tcggtgcgcc atcgggcata gtcggggtgg tcgggggtga tgcctgtgcc
     6301 gagcttgagc agtcctgccg cgccgtggct ggcttgggtg cggatctcgt ccatgctttt
     6361 tgcaggtagt ccgtggcgag cgagcagggt gttgagtgcg cctccgaggt cgagggcggt
     6421 gtcggcaagt gtgccgtcga ggtcgaagag tacggcgtgt atcatgtgtg ttcctttttt
     6481 ataaagtgcg ggacgaaggg tttcagacgg catgtttatt ttgtttcaaa ccctgttcga
     6541 aatcttccag catatccaat tcaaagcggc tgaagcccgc tttttcgcgc gcttcgatgt
     6601 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggtaa
     6661 tcggttccaa accgcgttcg cggcagaggt gttgatacca atggttgccg atggcgacat
     6721 ggcccacttc gtcgcggtaa atgatgtcca atacgccgca ggtttccgaa tcgccgcgct
     6781 gcgccacctt cgcgcgtatc cccggcgtaa cgtccagccc gcgcgcttcc aacacgcgcg
     6841 gtaccaacgc catacgcaac agtgggtcgt aggcggtttt gtacgccata tcccacaaat
     6901 ggttgtgcgc ctcgaaactg ccgtaatcga agccgaaagc gcgcaatctg tcgcgcatca
     6961 ggcggaaatg gtacacctct tccttcgcca ctttcaccca gtcgcggaca aactgaaacg
     7021 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     7081 gcgcaatcgc gtgcagcatc gccgcatagc cttctgccgt gttcattttg cgcggggtca
     7141 gttgtgacgg cgcgaccaaa accggcttgt ccggccgccc tgcgcggggg aaatccagcg
     7201 gcggcgtgtt tgtttcagca ccgtccatgt tctgaacggc ggcaaacgcc tcatccgtca
     7261 gccgtccttt ttcatctggg tcgcccgaaa gcagggcgcg ttccagcaaa gcataaatat
     7321 cgagtttcat ctcaagtccg ccgtgttcgg aaaacaaata ttatagcgtt ttaaaaaaac
     7381 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     7441 gattaacaaa aaccggtacg gcgttgcctc gccttagctc a
//
LOCUS       DAWGJV010000103         7454 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWGJV010000103 DAWGJV010000000
VERSION     DAWGJV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.103"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            156..329
                     /locus_tag="WHQ94_001514"
     CDS             156..329
                     /locus_tag="WHQ94_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ2128772.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVNRDAVGVI
                     RKEAGKSLSQPEK"
     gene            393..1676
                     /gene="hemL"
                     /locus_tag="WHQ94_001515"
     CDS             393..1676
                     /gene="hemL"
                     /locus_tag="WHQ94_001515"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ2128773.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1892..2164)
                     /locus_tag="WHQ94_001516"
     CDS             complement(1892..2164)
                     /locus_tag="WHQ94_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ2128774.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2175..2738)
                     /gene="orn"
                     /locus_tag="WHQ94_001517"
     CDS             complement(2175..2738)
                     /gene="orn"
                     /locus_tag="WHQ94_001517"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ2128775.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="WHQ94_001518"
     CDS             complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="WHQ94_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ2128776.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(3761..5122)
                     /gene="accC"
                     /locus_tag="WHQ94_001519"
     CDS             complement(3761..5122)
                     /gene="accC"
                     /locus_tag="WHQ94_001519"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ2128777.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5235..5690)
                     /locus_tag="WHQ94_001520"
     CDS             complement(5235..5690)
                     /locus_tag="WHQ94_001520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ2128778.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5672..5929)
                     /locus_tag="WHQ94_001521"
     CDS             complement(5672..5929)
                     /locus_tag="WHQ94_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ2128779.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6046..7086
                     /gene="queA"
                     /locus_tag="WHQ94_001522"
     CDS             6046..7086
                     /gene="queA"
                     /locus_tag="WHQ94_001522"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ2128780.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1544 a         1868 c         2231 g         1811 t
ORIGIN      
        1 agacggaaga gggaatcgca caaacggttg ttaaaggcaa aacgacccgg gcggaggtag
       61 aggcacgttt cgggaagcgc aaccctttcg gttgttatgc ctatcatgaa gtcagcctgc
      121 cgatttataa ttttttgccg accaatttca tttatatgaa atcagaacgc cggcattggg
      181 agtggtgtgt ggattacgac ggggagggag ttgtccggga ctaccgcttt acacataaag
      241 tggaaaaaga cgaacgttcc gtcaaccggg atgcggttgg cgtgatccgc aaagaagcgg
      301 gcaaatcctt gtcgcaacct gaaaaatgat aaaatggggc tttctgcttc caagcccgaa
      361 acctgccgtt caggcggcat ttgaggataa atatgaaccg taacgaaatt ttattcgacc
      421 gcgccaaggc ggtcatcccc ggcggtgtga attcgccagt ccgcgcattc ggcagcgtcg
      481 gcggcgtacc gcgcttcatc aaaaaagccg aaggcgcgta tgtttgggat gaaaacggca
      541 cgcgctacac cgactatgtc ggctcgtggg ggcctgcgat tgtcggccac gcgcaccccg
      601 aagtcatcga agccgtgcgc gaagccgcgt tgggcggttt gtcgttcggc gcgcctaccg
      661 agggcgaaat cgtcatcgcc gaagaaatcg ccaaaatcat gccgtccgtc gaacggctgc
      721 gcctcgtcag ctccggtacc gaagcgacga tgaccgccat ccgtttggca cgcggtttta
      781 ccggtcgcga caaaatcatc aagtttgaag gctgctacca cgggcattcc gacagcctgc
      841 tggtgaaagc cggcagcggc ctgctgacgt tcggcaatcc ttcttccgcc ggtgtgcctg
      901 ccgactttac caaacacact ttagtccttg agtacaacaa catcgcccaa ttggaagaag
      961 cctttgctca aagcggcaat gacattgcct gtgtgatttt ggagcctttt gtcggcaata
     1021 tgaaccttgt ccgcccgacc gaagccttcg tcaaagccct gcgcgaattg accgaaaaac
     1081 acggcgcggt gttgatttac gacgaagtga tgaccggttt ccgcgtcgcg ctcggcggcg
     1141 cacaatcgct gcacggcatc atgcccgacc tgaccacgat gggcaaagtc atcggcggcg
     1201 gtatgccgct tgccgcgttc ggcgggcgca aagacattat ggaatgtatt tccccactgg
     1261 gcggcgtgta tcaggcaggt acattatcag gcaacccgat tgccgtcgcc gccggtttga
     1321 aaacgctgga aatcatccgc cgcgaagggt tttatgaaaa cctgacggcg cgtaccgaac
     1381 agttggttca aggttttcag acggcagcgg atgcggcggg catcgagttt gccgccgaca
     1441 gcgtgggcgg tatgttcggt ctgtatttcg ccgcacacgc gccgcgaaac tatgccgata
     1501 tggcgcgttc caatattgac gctttcaaac gcttcttcca cggtatgctc gaccgcggca
     1561 ttgccttcgg cccgtccgct tatgaagcag gtttcgtttc tgccgcgcat acgcccgagc
     1621 tgattgacga aacggttgcg gttgcggttg aagtgttcaa ggcgatggct gcatgatgtt
     1681 ttgacggaca gagtttctct gttcgatttg tttggcagat tgaagtaaga atgcacaccg
     1741 ccgtcattcc cgcgcaggcg ggaatccaga cctttcagtt tctgtaatga ttgaaaataa
     1801 cgggccgaaa tcagccgcgc ccggcaggaa gccgccgcca cctgaaacaa tgccgtctga
     1861 agccttcaga cggcatttta tcggaagcgc atcagaaaaa acggatagcg acatatgcgg
     1921 taaaagcaag ggcaattaca tagataaacc cctttataaa ttcaaaagtc tcatctatat
     1981 cgctatattt ttctttcagt tcgtcagatt ttcgataacg tttcattttc aagaccactt
     2041 catgaccaac aagcccgacc ttccggattc ccgcctgcgc gggaatgacg ggcgtgtata
     2101 tttttgattt caatctgctg taaaaatgcc gtctgaaatg caggatttgc gcttcagacg
     2161 gcattttttg tttcttattg cgcttcggct ctcggggcgg aaatcagaaa gtgttcgcgg
     2221 tagtggcgca tttcttcgat gctctccaaa atgtcgtcca atgccttgtg cgaaccgcgt
     2281 ttgacgacgc ttttggcaac gggcggattc cagcgtttgg caagctcttt cagtgtggaa
     2341 acgtcaaggt tgcggtagtg gaagtagttt tccagtttcg gcatatattt gaccataaaa
     2401 cgccggtctt ggtggatgga attgccgcac atcggcgtgg cgcgtccggg tacccattcc
     2461 gacataaagt ccagcagttt ctgttcgact tcggcttcgg tatgcgacga ttcgcgtacg
     2521 cgctgtgtca gccccgtcct gccgtgtgtg gcggtgttcc actcgtccat attgtcgagc
     2581 agctcgtcac tttggtggac ggcgtaaact tcggattgcg ccaatacatt caaatccgag
     2641 tcggtaataa tcatcgcgac ttcgataatg cggtcggttt cgggattaag ccccgtcatt
     2701 tccatatcga gccagcagag gttgtttttg tcctgcacgg tgtttccttt ccgtttcagc
     2761 gttttgtccc gctcaagcgc gcccagcttt cttcggtttc cgccgggtcg agatcgaacc
     2821 attggctgta aatgccgccg agttcttcgg cttgttcgtc caacaaaccg gataacacga
     2881 tgcggccgcc ttgtttggtg cgggcggcga gcatttcgcc gagcatacgt aaagggttgg
     2941 cgaggatgtt ggcgacaact acgtcgaatt gcccttgagg cagaccgtcg ggcaggaaga
     3001 attgtgcatc gacgttgttc tgttcggcgt tgtccttgcc ggcgcggacg gcttgttcgt
     3061 caatatccac gccgacggca gaacctgcgc cgagtttgag ggcggcgatg gtcaggatgc
     3121 ccgaaccgca gccgtagtcg aggacgcttt cgccgttttt gagttgcgta tccagccatt
     3181 tgaggcagag gcgcgtggtc gggtggctgc cggtgccgaa ggcgaggccg ggatcgaggc
     3241 gtaggttgac ggcagtgcct tcgggggctt cgtgccaaga gggggtaatc cacaggcgtt
     3301 cggaaattcg gatggggtcg aattgcgctt gggtcaggcg tacccagtct tggtcttcga
     3361 gggtttcgcc ggtgtatgcc aagtctttta acccgcattc ttgtgcggcg gtttggatga
     3421 tggcggcggc ttcgtcgtgt tcgccgaaca gggcgatgac tttgctctgc tgccagattt
     3481 gttcggcggg cataccgggt tcgccgaaaa tcgcctgttc gttttgcgtg ccggcgtagg
     3541 catcttcgat ggcggcggag agtgcgccgt gttccatcag cgcgtcggcg aggcgttcgg
     3601 cgacggcatc gttgacgttg acggtgattt gttggtaggg cataacgggc tttcttaaca
     3661 gaccttcgga atagggtcgt actgtataag gggatgggtg tgtaacggaa atgccgtctg
     3721 aaaacggaca aactgtttca gacggcatat cggcggcggt ttacttgtcc tgtttggctt
     3781 tgcgatcttc cagccagtgt tccaaatagt ggatgctgac gccgcctttt tggaaacccg
     3841 catcggcgaa caggtcgcgg tgaagcggcg tattggtttt gatgccggtt accgccagct
     3901 cggcgagtgc gacgcgcatt ttcgccattg cctgttcacg cgttttgccg tgtacgcaga
     3961 ttttgccgat caggctgtcg tagtacggtg ggatgcggta gccttggtaa atgtggctgt
     4021 ccacgcggat accgaagcca ccgggcaggt ggcagctttc aatcgggcct gggctgggga
     4081 tgaagttgta cgggtcttcg gcgttgatac ggcactcgaa agcgtggcct tcgattttga
     4141 tgtctttctg tttgtattgc agcggcaggc cggatgcgat gcggagttgc tcctgcacga
     4201 tgtccacgcc ggtgatgagt tcggtaaccg gatgctcaac ctgaacgcgc gtattcatct
     4261 cgataaagaa aaattcgccg tcttcgtaca ggaattcaaa cgtacctgcg ccacgatagc
     4321 cgatgcgttt gcaggcgtcg gcacaggcgt tgccgatttt ggcgcgttct ttttcagtga
     4381 tgaacggagc cggtgcttcc tcgatgactt tttggtggcg gcgttgcagc gaacagtcgc
     4441 gctcggcaag atatacggcg ttgccgtgtt cgtcggcaat cacttggatt tcgacgtggc
     4501 gcggacgttg cagatagcgt tccatgtaaa ccatcgggtt gccgaatgcc gcgcctgctt
     4561 ctgccttggt catttcgaca gattggagga ggtcttcttt tttctcgacc acgcgcatac
     4621 cgcgaccgcc gccgccgcca gaggctttga taatgacggg ataaccgact ttatcggcga
     4681 ttttgaggat ttcttcgccg tcgtcgggca atgcgccgtc agaaccgggg acgcagggta
     4741 cgcctgccgc tatcatcgcg tgtttggcgg agactttgtc gcccatcagg cggatggtgt
     4801 cgggtttcgg gccgataaag gtaaagccgg actgctcgac ctgttcggcg aaatcggcgt
     4861 tttcggcaag gaaaccgtag cccggatgga cagcgtccgc gcagcttact tcggcggcgg
     4921 cgataatggc ggggacgtta aggtagcttt gcgcggaagc ggcagggccg atgcacacgg
     4981 attcgtcggc gagtttgacg tgcaggctgt ctttgtcggc ctcggaatgc acggcgacgg
     5041 tggcaatgcc catttcgcgg caggcacgga gtacgcgtaa tgcgatttcg cctcggttgg
     5101 cgattaaaac ttttttcagc atgatgactg ttcctgtggt tccggcaacc cgtccgtaaa
     5161 aaaaaggtgc gggaacgctg aagggggaaa gcatttcaga cggcatcgga agtttatgcc
     5221 gtctgaaaac aggattatcc gatgatgaag agcggttcgc cgaattcgac gggcgtaccg
     5281 ttttcgacca aaatttcttt gaccgtgccg gatttttcgg cttcgatttc gttcatcagc
     5341 ttcatcgctt cgatgatgca cagcgtgtcg ccggctttaa cctgttggcc gacttcgaca
     5401 aaaggcgcgg cattcgggcc gggtgcgcgg tagaacgtgc cgaccatagg tgatttttgc
     5461 gcgtcggaca aatcgcgggc ggcaggtgcg gcggcaggcg cggatgccgc aacgggtgcg
     5521 gcggcaggcg ttacggccgg cgcggcggca ggtacgggcg cggcgtaaac gggtgcggcg
     5581 gcgatggttc gggtgatgcg gactttttcc tcgccttcgg ttacttcgat ttcggcgata
     5641 cccgattctt caaccaaatc aatcagtttt tttaatttgc gcaaatccat ttctgttcct
     5701 ttaaaggctg ccggtgttcc ggcgggttgt tgcgttggtt tttcagaaaa cagccggctt
     5761 gcggcaacgg cgcaaaccgg gcggaatggt gttcggatac ggttttcctg tggtggaaaa
     5821 caaccgtgag aatgtgttta ttttcccgaa gttgtcggta aatgtccagt aaaatatcaa
     5881 aaaacggcgg tttttggggg aaatgtgcag gaagtttgat tttgcgcaca agatgttcca
     5941 ggttaagagt gtttattcta atctgttggt ttttcggaca aagatgccgt ctgaaaaagg
     6001 gctaaagtgc gtataatggc ggcttgcccg aacgagagtg taaaaatgga tatttcagat
     6061 tttgatttta ccctgcccga aaagctgatt gcccagcatc cgcccgaagt gcgcggcagc
     6121 agccgccttt tggttgcgtt gcccgatatg ccgctgcaag accgggtgtt cggcgatttg
     6181 ccggattatg tcgaggcggg cgacgttttg gtgttcaaca acaccaaagt catgaaggcg
     6241 cggctgtttg ggcagaaaga cagcggcggc aggatcgaag ccctgattga gcgcgtgttg
     6301 gacaaccata ccgcactggc gcacatccgt tcgtccaagt cccccaagcc cggtatgggg
     6361 ctggtgtttg aaggcggcat ccgtgccgtg atggtcgggc gtgagggcga actgttctgc
     6421 ctgcgttttg aaggcggtca aaccgtttac gaacttttgg aacagaacgg acacctgccc
     6481 ctgccgcctt atatcgaacg cgccgccgac gcggacgacg acagccgtta tcaaaccgtt
     6541 tatgccaaat atcagggtgc ggtcgccgcg ccgacggcgg gtctgcattt tacggaagaa
     6601 cttttgcgcc gcctgaaaga caaaggcgca gtaaccgcag aagtaaccct gcacgtcggt
     6661 gcgggaacgt tccagcccgt gcgtgtggaa aaaatcgaag aacacaaaat gcacagcgaa
     6721 tggtttgaag tgccgtctga aaccgccgcc gccgttgagg cggcaaaagc acgggggaac
     6781 aaagtctggg cggtcggcac gacttccatg cgcgccctcg aatctgccgc gcgtgcgacg
     6841 ggtcgtctga aagcggggca gggcgacacc gatattttca tcacgccggg ctaccgtttt
     6901 aatgttgtcg acaggctggt taccaatttt catttgccga aatcgacgct gctgatgctt
     6961 gtcagcgcgt tttcgggtat gggtcatatc cgcgccgcgt atcgttatgc ggtcgaacgc
     7021 gaataccgtt tcttcagcta cggcgatgcg atggttttgg ggcggaacga aggggtggta
     7081 cgttagaaca gccccaagtc tattggggga agacaagtgc cgtctgaacg gacgttcgga
     7141 cggcattctt ttgttgattt gaatgaaagg aactgtatga catagattca ggcgcgagag
     7201 gcgagaaact cagtcattgt tgatgacgcg caatacgtta aaattcattc cgttttggtc
     7261 gtataaacat attacatacg ccattttttt gtattcaagg ctgcctgaaa acagtgggca
     7321 agaatatcta acccaacgaa aagctgaaaa agcaacaaaa aataatctgc accttagtcg
     7381 gttggttagt ccaaccttag gggtgcagac cattcaatct ttcaggctgc ctttgtttgt
     7441 ttataagata aatt
//
LOCUS       DAWGJV010000104         7386 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWGJV010000104 DAWGJV010000000
VERSION     DAWGJV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.104"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WHQ94_001523"
     CDS             complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WHQ94_001523"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ2128781.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2961..3755)
                     /locus_tag="WHQ94_001524"
     CDS             complement(2961..3755)
                     /locus_tag="WHQ94_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ2128782.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFAQAWK"
     gene            complement(4505..5374)
                     /locus_tag="WHQ94_001525"
     CDS             complement(4505..5374)
                     /locus_tag="WHQ94_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ2128783.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFRHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5425..5961)
                     /gene="ruvC"
                     /locus_tag="WHQ94_001526"
     CDS             complement(5425..5961)
                     /gene="ruvC"
                     /locus_tag="WHQ94_001526"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ2128784.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASQLNPSGMQVKRGRFQ"
     gene            complement(5964..6203)
                     /locus_tag="WHQ94_001527"
     CDS             complement(5964..6203)
                     /locus_tag="WHQ94_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ2128785.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6233..7243)
                     /gene="dusB"
                     /locus_tag="WHQ94_001528"
     CDS             complement(6233..7243)
                     /gene="dusB"
                     /locus_tag="WHQ94_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ2128786.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
BASE COUNT         1658 a         1965 c         1934 g         1829 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataattaat atgactttgc ggccgttttg
       61 ccattgcgta ataaaacgat ggggaagtga tgataaaacg tgtgtgtaac tatatcagac
      121 ggcattgttt ttctgtttga cggcctcaat ccaaaatttt gccgacgatt tcgcccacgt
      181 ctttcgacaa tccttcctgc gcccgaatgc gctgcaatgc ttgtttcacc aagtttttgc
      241 ggtgcggctc gagcttgttg cagaggttga acgcctgcac caggcgggcg gcgacctgcg
      301 ggttaaagcg gtcgatttcg atgactttgt cggcgatgaa gcggtagccg ctgccgtctt
      361 ctgcgtggaa atgcgggacg ttgcggctga agctgccgat gagcgagcgg gctttgttgg
      421 gattttcgag gctgaacttc ggatgctgca aggcggtttg aacctgttgc agggtgtcgc
      481 tgcggcggct tgagccgacg agggcgaaat atttgtccat caccagcgcg tcgtctgaaa
      541 acttgtcggc aaactgcgcc agcaggcggt tgcgcgtatc gctttcgttg ccgttgacgg
      601 cggacaggat gccccattcg tgggtcatgt tttgcgccat ttcggcgtat ttctcggcaa
      661 cggtttcgat gtgcgcggga tcggcgcgca ggacgaaggc gcggcagaca ttgcgcagcg
      721 tgcgccaacc ggcggcttcg gggctgtact cgtagctttg gttttcctgc ttcgccgcct
      781 gacggttcaa ttcgtgccat ttcggcagaa agcggacggc aagtatatcc aacaaggctt
      841 cgcgcgcctg atggtagcgc agcgggtcga tgttttccgc gccgtcccac agctcggctt
      901 cagacggcac acccaaaagc agggctttga aagcgttgtc taagaggtcg tctgaaatga
      961 ctttttcgac ggcggcaagc agtttttcgt gtttcggcaa cccgatgccg tctgaaagcg
     1021 cggcaaggtt ggcggcgacg gcacggcggt agagcgtttg ggcggcttcc cagcgcgtga
     1081 aggcgtcgct gtcatgggcg agcagaagca gcaggtcgtc gtcgctgtac ggatagttca
     1141 gatgcaccgg cgcgctgaag ccgcgcagca gcgagggaac gacggcttcg gttacgcctt
     1201 cgagcaggaa gatctgttcg gcttcggtca gcagcaatac ggcttcggtc gcgcgtttgc
     1261 cctgataatc gaatgccgcc gcttcgccgt tgcggttcag cagcccgatt ttgacgggaa
     1321 tcatcatcgg ctgcttgtcc gccatatcgg gcgtgggcgg cacggtttgt ttgatggtta
     1381 actcgaaact attgtttttc agacgacctt ccgcttccaa gacgggcgta cccgcctggc
     1441 tgtaccacaa ggcgaactgg tcgagattga tgccgttcgc gtccgccatc gccgcgcgga
     1501 aatcgtcgca ggtcacggcc tgcccgtcgt gtcgttggaa atagagcttc atgccttttt
     1561 ggaagccctc ttcgccgagc aaggtgtgat acatccgcac cacttccgcg cctttttcat
     1621 aaacggtcat ggtgtagaaa ttgttcatct cctcatagct ggcggggcgc accggatggg
     1681 cggtcgggcc tgcgtcttcg gggaactggt gctggcgcag caggcggatg ttctcgatgc
     1741 ggcgcacggc gcggctggcg cggtcgccgg aaaattcttg gtcgcggaac acggtcaacc
     1801 cttctttcag cgaaagctgg aaccaatcgc ggcaggtaac gcggttgcct gtccagttgt
     1861 ggaaatactc gtgtccgacc acggattcga tgccttcaaa atcggtatcg gtggcggtac
     1921 ggctgtcggc aaggacgaac ttggtattaa aaatgttcag ccccttgttt tccatcgcac
     1981 ccatattgaa atcgcccacg gcgacgacca tgaaaatatc caagtcgtat tccaaaccga
     2041 agcgcgtttc gtcccacttc atcgcgtttt tcaaggattc cacggcaaag ccgaccttag
     2101 gcttgtccgc ctcggcagtg taaaactcga ttttgacgtt tctgccgccc atcgtggtga
     2161 aacagtcttc cgtgaccgcc aaatcgcccg cgaccaaagc aaacagatag ctcggtttgg
     2221 caaacgggtc ttcccatttc acccaatggc ggccgtctga acactcgccg ccgtcgattt
     2281 tgttgccgtt ggagagcaaa acgggatagc gttttttgtc cgcaacgatg gtggtcgtga
     2341 atttggacat cacatccgga cggtcgatgt agaacgtgat tttgcggaag ccctccggct
     2401 cgcattgggt aaacagattg ccaccggaag catacagccc catcagcgat ttgttttccg
     2461 ccggcaggat ctcggtttcc acttcgacgg tgaagcgttc ggacggcacg tctgcaatcg
     2521 tcagcgtctc gccttccaac acataatccg ccgccacgcc gttgattttg acggacaaga
     2581 gtttcgtcga accgtccaac accagcggct cccccaccct ctgcggctcg accgtcaaac
     2641 gggatttcac gacagtttgc ggttcggcaa tatcaaaatg caggtcggtt tcaaggatgc
     2701 ggtaggcagg cgtttggtaa tctttcagat aacggacggt tttgctcatt tttttctttc
     2761 aatattgttt ggtttgactg gaaaaggctt cagacagcac agatacagct tttaaagcgg
     2821 attaacaaaa atcaggacaa ggcgacgaag ccacagacag tacagatagt acgggctgta
     2881 ccggtttgaa tttaatccac catatgccgt ctgaaaccga aacggcggca caagcgtacc
     2941 gccttcggac agcaggtttt ctatttccac gcttgggcaa attctgccca atgcggtttg
     3001 tcttctgttt gggcggaaag atagtttctg atccgcttga tttccatttc gtattcgtcc
     3061 gcgtcgaatc tgccgctgac cagacaaaaa cgcagataca tcaaatacgt attcacggca
     3121 tcggtttcac aataattacg gatttccttc agcctgcccg cgtggaacgc ctcccacacc
     3181 ttgctgccgt ccatacccaa tttacccgga aaaccgcaca gtttcgccat atcgtccagc
     3241 ggcacattcg ccctcggctg gtaaagtgcg agcaaatcca tcaaatcgca gtggcgttgg
     3301 tgataacggc tgatgtagtt gttccacttg aaatcgcggc tgtcgccgaa atcgccgtcg
     3361 cccatatccc aatagcgcgc ggcgttgatg ccgtgtagca gcgaacggta atgcagaacc
     3421 ggcaggtcga aaccgccgcc gttccaactg accagttgcg gcgtatgttt ttcaatcaac
     3481 tcgaaaaatt tggcaatcac gacttcttcg ccatcgtcca tctcgccgat tgtaccgaca
     3541 tggactttat cctgccccca acgcatgcag cacgaaatcg ccacaatctg atgaagatga
     3601 tgctgcataa aatccccacc cgtctgagca cggcgttttt gctgggcaaa cagcaccact
     3661 tcatcgtcgg gcagcgagga cggcagctcg tacaatgttc ggataccctg cacatcgggt
     3721 acggtttcaa tatcgaaagc caaaatcgtg gtcatgacag caccttgtat ttaaaacaga
     3781 cttgcgccta ttgtgtcatt aaaaggcgga taaaaaaaga ggcaaccccc cacaggattg
     3841 ccccaatacc tcaaatcaga gatttacgct tcacaaacaa tacaggcttt cgcctgcggc
     3901 tttgcccgcg tagctcaact ctacgccggc aaacttttgt ttcaccgttt ccgatgaaac
     3961 cccgaccaat cgcaagattg accggaaaat cctttcagac ggcatttcct gcctgtcgtg
     4021 taattccatg tagcggaatg tacgccattt tctacgcttt gccaagcatt ttttacaata
     4081 taaatgtcaa aacattaatt ttataaaatt gctgaaaata ttaaatatat gaatttttat
     4141 tttaaatatt ttaataaata taaatttaat tttgatttat atttaaattt aagcataaaa
     4201 tgttaaatat taaaggcaat atgaaaggca tatattaaat atttatttat aacgctatgt
     4261 ttttaaagaa aattaatttt aatatattaa ctagattgtc tgcagatatt catgggtttg
     4321 cggtatttct tccaaaacct gcttcgaatt ttccgaccaa gtcttaaaaa tattgttttt
     4381 tgagatactt aaatagcagc gattatcaaa tgaaatctgt tcatataatc tgccatttcg
     4441 catttaaaaa aacaaacagg agtttcgact cgaaacgcct gatatgtttt gtaattttac
     4501 gtagtcagta aaaatcgggg ctgccttccg gacgggtttt gaaacgcttg tgcagccaaa
     4561 aatattgttc cggatgttcg cgcacccgct cttcgataaa gcggttcata cgctgcgcgt
     4621 cggcctgcgc atcttcactc ggaaaggatt cccaagcagg gtagaaatgc aatgtaaccg
     4681 tattgtccgc ctccctgacg ggaatggcgg gtatcacttt tgcatttgca agcgcggcaa
     4741 tgcggctcaa tccggtaatc gttgccgtct gaataccgaa aaaatccaca aaaaccgaat
     4801 cgttgcgtcc gaaatcctga tcgggcagat acaggaacgg cgcgctgctt ttgcggaact
     4861 gtttgactag agcgcgcagc ccttctgtgc gcccgataag gaagacattg tcgtagcggt
     4921 tgcgaccttt caaaatctgt tcgtccaata tcttgttttt ttggtgggaa tacatactga
     4981 tcagcggaac atcctgatta agtgcgtaca ccgccatctc gaacgcggta aagtgcggat
     5041 acaggatgat gactttttcc cccgccgcca gcgcgtcgtc caaataatgc ttattgcggt
     5101 agcgcaccag cgatttcaaa cgtccggcag gcgcgtacca atataaaccg tattccaaca
     5161 tcagtttcgc catgtgcctg aaatgctgtt tcaacacggt tttacgcttt tcctcactcc
     5221 attcggaaaa acatttcgcc aaattgattt cgccgatacg gcggcgcggt ttgaccagaa
     5281 ggtaggcaag caaacccgtc aggtcggcaa tcttgtgcag cagcgcaaac ggtagaaact
     5341 gcaaaacata cagtacaaaa aatataaatt tcatctcgat acacattttc ttttcagacg
     5401 gcaaaataca aatgccgtct gaaactattg gaaccttccg cgcttgacct gcatccccga
     5461 cgggttgagc tgggacgcaa gcccgtggtt gcgtaaggcg tgggtcagcg cgacggcaag
     5521 accgtccgcc gcatccgcct gcggtgttcc cgaaagcccc agcatctgca ccaccatatg
     5581 ctgcacctgt tcttttgccg ccttaccctt gccgactacc gcctgtttga cctgcaaggc
     5641 cgtgtattcc gaaacgggca gcttatggct gaccaatgcc gccaatgccg cgcccctagc
     5701 ctgacccagc atcagcgtcg atgccggatt gacgttgacg aacacctgtt ccaccgccgc
     5761 ctgctgcggc ttgtaaacgg caacgacttc gccgatgtgc cgcacaatca cggcaatcct
     5821 gtctgccaga ggcgcatcgg gaggcgtttt gatgcagccg gaggcgacgt aaaaatgatc
     5881 gcgccccctg acatcgatga tgccgaaacc cgttacgcgg ctgcccgggt cgatacctaa
     5941 gatacggacg gttgccgaca tattcacagc aaaccgtgtt gaatcagttt tttacgcaaa
     6001 gtattgcggt tcagtcccaa catgacggag gctttggact ggttgccgcc gcattgctcc
     6061 atcacgcaca ccagcagcgg cttttccacc tgatgcaata ccatatcgta cacgccgcaa
     6121 ggttcggtac cgttcaggtc tttgaaatat tgttccaaat tttgtctgat acattgggaa
     6181 atatcgggaa gggtatgggg catgattgca ctttcaaagg ataatcaagt gttcagaagg
     6241 catttgtgcg gtagccgcac gcccaacggt cggtttttcc ggcaagtctt tcaagataac
     6301 ctgcaagcat gtcgtattgc gccgccgcac tgtccaagcg gttgatttca cgccgcgcct
     6361 gttcgccgtc gggcatttcg ccgatgtacc aacctatgtg tttgcgtgcg atgcgtacgc
     6421 ctgcactatc gccgtaaaac gcgtgtatgg cgcggatgtg gttcaaaata gcggcggcgc
     6481 attctgccaa actcaaggca ggcggtaaaa caccgtgttc ggcgtaatgt ttcaaatcgc
     6541 ggaagaacca cggtctgcct tgcgcgccgc gccctatcat gatgccgtcg gcggcggttt
     6601 gtttgaggac ggcggcggct ttttgcggcg aagtgatgtc gccgttgacc cagaccggga
     6661 tgttcagacg gcatttggtt tcggcgatga gttcgtaacg cgcctcgcct ttgtacattt
     6721 gcgtgcgcgt gcgtccgtgg acggcaaggg cggcgatgcc gcaatcttcg gcgattttgg
     6781 cgatgacggg caggttttga tggtcttcgt gccagcccaa acgggttttg agggtaacgg
     6841 gtacgcctgc cgcacggacg acggcttcca aaatggcggc aaccagcggc tcgttctgca
     6901 tcagcgcgct gccggcttgg acgttgcaca cttttttggc gggacagccc atattgatgt
     6961 cgataagctg cgccccgagg ctgacgttgt aacgcgccgc atccgccatc tgctgcggat
     7021 cgctgccggc aatctgcacg gcaacgatgc cgccttcatc gacaaaatcg ctgcggtgca
     7081 aggtttttct agtatttctg agcgtcgggt cgctggtcag catttcgcac actgcccaac
     7141 ccgcgccaaa ctcgcggcaa aggcggcgga agggtttgtc ggtaatgccc gccatcggcg
     7201 caagtgcgat ggggttgtcg ataaaatagc cgccgatgtg cataatgggt ccgcgtttca
     7261 aaaaagtgcg acattgtaca ttttttaagc aggatttcca atctccggac gcgcccgcga
     7321 ttggggcggg caccgtttta tggcataatc cgcacacaga tttcctgccc cgccactcac
     7381 aggcgg
//
LOCUS       DAWGJV010000105         7334 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWGJV010000105 DAWGJV010000000
VERSION     DAWGJV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.105"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..164
                     /locus_tag="WHQ94_001529"
     CDS             <1..164
                     /locus_tag="WHQ94_001529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128787.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            201..413
                     /locus_tag="WHQ94_001530"
     CDS             201..413
                     /locus_tag="WHQ94_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ2128788.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            561..2144
                     /locus_tag="WHQ94_001531"
     CDS             561..2144
                     /locus_tag="WHQ94_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ2128789.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2226..3323
                     /locus_tag="WHQ94_001532"
     CDS             2226..3323
                     /locus_tag="WHQ94_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ2128790.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALAQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAKHLRRFASRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3437..4552
                     /locus_tag="WHQ94_001533"
     CDS             3437..4552
                     /locus_tag="WHQ94_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ2128791.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEAMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4677..5132
                     /locus_tag="WHQ94_001534"
     CDS             4677..5132
                     /locus_tag="WHQ94_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ2128792.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDYIIAMDGNNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5203..5679
                     /locus_tag="WHQ94_001535"
     CDS             5203..5679
                     /locus_tag="WHQ94_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ2128793.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5667..6593
                     /locus_tag="WHQ94_001536"
     CDS             5667..6593
                     /locus_tag="WHQ94_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ2128794.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6658..7215
                     /locus_tag="WHQ94_001537"
     CDS             6658..7215
                     /locus_tag="WHQ94_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2128795.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1926 a         1801 c         1909 g         1698 t
ORIGIN      
        1 atgcgccgat tgccaaggcg atgatgctca accagggttt ttgcgaagcg ttcattgttg
       61 tcgtcctgat gggaattgcc gccattatgc gcaagcgtcc gtctttttcc aaaccgccgc
      121 accgtttccg cgtttgccca agttcacgcc ccgacttaca ataacgcccg tcagattgca
      181 gttccaacga aaggaaaatg atggaaaccc ttatcctcga catcggcggc atgagctgcg
      241 gcggctgcgt caaaagcgtt acccggatat tggaaggcgt aaaaggcgtg gcaagcgtcg
      301 aagtcagtct tgaaaacaaa agtgcgaccg tcggatacga ccccgcgcaa accgatgcag
      361 gggcgttgat tgaagccgtt gaagacggcg gctatgatgc ggcgttgaaa taaagcggca
      421 aaaatgccgt ctgaagcctt cgcgcctccc cgatgccgtc tgaagtacgc tccgcgcctt
      481 cagacggcat ttgatttagt tgaacgggta ggatgaattg gttaaacttg cgggcaatag
      541 tgccttcaga caaacaaaat atgccgaaca aaaagaaaaa agccgccttt gcgcgtgctg
      601 caacggagaa cggtgtgccg gacgtgtcca acatcaaaaa ttcatatgat gatttgatgt
      661 atgaatccaa agcattttcc caaaccgcca tcaacgcctt ggaggctcgc gcccgcatga
      721 tgggtttgca gcccgcgccc gccgccaatg ccaaggtctt ggagctgggc tgttcgatgg
      781 gcggcaacat catcacgcag gctctttatt acccggatgc ggaatttgtc ggtatcgact
      841 tgtccggcag gcaggttgcg cagggcaacg ccatcattga aaaaatgggc ttgaaaaatg
      901 tgcgtctgga agaaaaagat attttgaccg tcgatgagtc attcgggaag tttgactata
      961 tcatcgtcca cggcatttgg tcgtgggtgc ctgacgcagt taaagacaaa attttttcga
     1021 tttgccggaa caacctgacc gaacacggca ttgcctatat ttcatacaat gtttaccccg
     1081 gctggaaacg gcaggagcag ttgcgcgaaa ttatgtactt tgccggcagg gatctgcttg
     1141 aagaaccctt ggaagcgcgg acgcggaaag gcttggacgc gctcaaggcg ctggcggaaa
     1201 ttttggaaaa cgacaaaggc ttgggcggcg gcggcaaact tccggcgatt caaaaaatat
     1261 tgaatcacaa tacttattat gtcgcacacg aatatatgga aatattcaac gacccgattt
     1321 acgttaacgg cttcatcgaa tgggcaaacc gccaccggct ggcgtatatc ggggataccg
     1381 atttgcacgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attttggcat
     1441 tggcagggga tgattatatt gccaaagaat tttatagcga tattttatcc gaccgccaat
     1501 tccgccgttc gcttttatgc cgtgaggaag tcggggacaa tatcaggcgt gatgagtcgg
     1561 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acgatcaact
     1621 ttgatgaaaa cgataccctg ctttcgggca tacgcgatgt gatgaaaacc ggagagccgt
     1681 ttaaaacgga agatgttgcg gaaaacctcg cccgccgatt tcccggtctg gaatttgacc
     1741 gtatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
     1801 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgtttta
     1861 cagattatgc cgccgccttt gtggaacacg gcgcggaagc gtttgttcgg cctgccaacc
     1921 gttacaacga aagcaccccc tcattcggat acgggcattt gtacattatg cggcaattat
     1981 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
     2041 gcaccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
     2101 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccgacg
     2161 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaag cggtacacga
     2221 cacatatgaa ccatacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
     2281 aaaaccacct gctgctcaat gcctaccagt tggcgcataa ttcttcccag gcttcgcgca
     2341 aactctcgtc cggccaactt caaaccgaaa tcaggggaat gcttgagcaa aaccactata
     2401 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
     2461 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
     2521 cggtcgtgct ggtgtccggc tgcaagaaag aacgggcaat cgagatgaag ctgccgacgg
     2581 aggcattgtt tgcctgcctg caaaactatc cgcacctgcg cgcgttggcg caagagacgc
     2641 aatggctgcc ttatcttgtg cattcgtccg atttgagtgc ggtcgcgccg gatgagtggt
     2701 ggcgtgccaa acaaaatacc gaagcggcgg cgaaacactt acgccgtttc gcctcgcgcc
     2761 ctttgctgtt gcccgaaggg cagtccgtcc acgtcgttta cgcgctgggt ttcggtagcg
     2821 gcaaagttca gacggcattg ggtcagaacc ttcttcaggc aggcctgccc ctgatgcagg
     2881 tatggcagga aaatcttgca tcggaaggcg ttacgctgtt tgccaatcca ctttccccgg
     2941 attctccggt acgcgcgctt tcagacggca gccacacgcg ccaacgtatg gcgatggatg
     3001 tgtttgcggc aaacgcgata cgcgccgtcc gtatgcagag tccgcgcgtc ggcgtggtcg
     3061 ctgcggcaaa ggcgggcggg caggttttat tcgggtttaa tgcgaccgac ggcgcgtttg
     3121 aagtcgtgcc gcaggtgttt tcgtggcagc tttcctttac cgacaatatt ggcgttattc
     3181 agcaaaattt tttggattta atggcagaat gccgtgtaga acacgtttac ctgttgcaca
     3241 atcccttggg cgaacaggaa agcatcccga gctatgcgga agcattgaaa cgggaggggc
     3301 acaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttatataa
     3361 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
     3421 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
     3481 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
     3541 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
     3601 tcttgaccta gaagaaaaat gtcttcaagt gacaggacct tttggacaaa aagaagccat
     3661 gcgctatttt caaaaagaac aactagccct attcgaagtt gctgatttgg ttggtcttgg
     3721 aaaaatcccg ctagagaaac gaaatccatt acaaatccaa actcgtggta ttggagaatt
     3781 gattcgccac ctcattagtc aagaaattaa agaaatctat attggcgttg gcggtacggc
     3841 cagtaatgat ggaggtattg ggattgctgc tggtttaggt tatcaatttt atgatgagga
     3901 tggaaatacc ttacccgttt gcggtcaatc cttactaaac ctagcttctg tttcaacaga
     3961 aaatcgctat gaaattcctg aagatgttca cattcgtatt ttagcagatg ttgtgagtcc
     4021 cttatgtgga caccaaggtg cgacctatac gtttggcaag caaaaagggt tggattctac
     4081 tatgtttgag gccgtagatc aggcaataca agatttttat gaaaaagtct cccctgcaac
     4141 attaaaactt aaaggagcag gagctggtgg aggcatcgct ggtggtttgt gcgcctttgc
     4201 tcaggcaagt atcgtatctg gaattgacac ctgcctggac ttgattgact ttgataagaa
     4261 agtgtcagat gttgacttgg ttatcattgg tgaaggaaga ctagatcgtc aaagtttagc
     4321 agggaaagcg cctattggtg tagcaaaaag aacccctgtc ggagttcctg ttgtcgctat
     4381 ttgtggcagt ctcgttgaag atttgccttc cctgccattt gaaaatatcc aagctgcctt
     4441 ttctattttg gagaaaagtg aacctttaga agatagtttg aaaaatgcca gtctctattt
     4501 ggagcatacg gcttctaata ttggacactt attaaatatg cctaagattt agccaaacca
     4561 tttcttccag atggatgttt tggctggttc tgccttatgc ggttacgatt aaccgaccga
     4621 tgccgtctga aagggtttcc gcttcagacg gcatcgcgcc ggaacaggga ggggatatga
     4681 aaaaaccgaa aatccttttt gtctgcctcg gcaacatctg ccgttcgccg atggcggaat
     4741 acattttgcg ccgccgcgcc gccgaagcgg gcattcccct tgaagcggac agcgcgggga
     4801 catcgggctg gcatgacggc gaggatatgc accgcgagac ggcaaagata ttgaaaaaac
     4861 acggtatcga tgcttcaggc tttaccagcc gcaaaatccg ccaaagcgat gcggcggcgt
     4921 ttgactacat catcgcgatg gacggcaata atttgtccga attggaaaga accttcggca
     4981 ggcggtcgga aaaaatattc aagctgaccg acctgatacc cgaaagcggt tacgaccatg
     5041 tccccgatcc gtggtacacg ggcgattttg aagaaacctt caggcttgcg gatgcgggct
     5101 gtcgggcatt gttggaaaag atttccaaat aaagcaattg aatacaaata taaaaccgcc
     5161 tcctgccgta tcggttgttc agacggcata aacagagaat ttatgaaaac aaatttcaaa
     5221 cagaaaatta tcgaacaggc acgcagcgaa ggcttgcagg taaccgcttt gcgcgagcag
     5281 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaacgt cttgtcgcag
     5341 atgcagcagc aaagcgaggg cgtgcttgca ccgcctaccg cctaccgcgc ccttgatttt
     5401 tgggcggagc agggcgtttt gcacaaagtg gcggcggtca acggctatat tttgtgcagc
     5461 cacgcgcagc acgagtgcga cgaccattgc cacgaccacg aagaagccga agcgcaccac
     5521 agcgcgttta ttttggtctg caccgaatgc ggcacggcgg acgagcaaac cctcagccac
     5581 gagtgggcgg cactgcgcgc aggcgttgcc gaaagcggct ttgcgctgaa agaagaacac
     5641 gttgttttaa ctgggatttg taaaaaatgt cagaaatgaa aaaaaccaaa gtccacctga
     5701 tttcaggttt tctgggaaca ggcaaaacca ccgcgctcaa aagcctgatg gcgcaaaaag
     5761 acccgaacga aaaatgggtc atcatcgtca acgagttcgg cgaaatcggt attgacggcg
     5821 cggtattgag cgacaacggc atccctgtgg cagaaatcgc cggcggctgt ttgtgttgca
     5881 ccgccggccc gcaaatgggc gtaaccgtgc agaaaatgct gcgcgacgcc aagcccgacc
     5941 gcctgatgat tgaagcaagc ggactggctc acgcagccag cgtcatcgac gaactcaaag
     6001 ccaaaccgct ggacagcctt ttggaaatcg gcgccgtctt taccgtcgtc aatccgcgcc
     6061 agttcatcaa ccccgattac gcgcaacagg cgttgtataa agaccaaatc ggcatctgcg
     6121 acgtattggt cgccagcaaa accgatttat gcaccaccga acagcttgcc gaatttcacg
     6181 acaaagcggc aaaactgttc ccgcccaaag ccaaagtggt cgaagtccaa aacgcacaac
     6241 tcgacatccg gtggctcgat attcccgtca tcgaaaaatc acgctaccgc ctcaaagccc
     6301 tgccggacaa caccatgggc ttccagtcgc aaggtttcac attccccgcc ggacgcgatt
     6361 tcgacggcga aaagctgacc aactttttca acgacctgcc caaaatgacc gaaggactcg
     6421 tccgcgccaa aggcgtgttt caagtattgg gaacgtgggt gtggctcaac tgggtagacg
     6481 gacagtgggg cgcgaaccaa gtctcatggc ggcgcgattc gcgttttgaa ttgattgcca
     6541 aatcgtttga tgcggattta atcgaacaaa agctcaaaga cgcattggaa tagcgttcac
     6601 gcgccttcag acggcatatg aaatgaaaat gccgtctgaa ggtctgagga ggacgggatg
     6661 aaacccaaat tcaaaaccgt tttaaccgcg ctgcttttgg cggtttccct gccgtctatg
     6721 gcggcaaccc atgttttgat ggaaaccgat atgggcaata tccgtttggt tttggacgaa
     6781 tccaaagcac ccaaaaccgt tgccaatttc gtgcgctatg cccgaaaagg cttttacgac
     6841 aatacgattt ttcaccgcgt catcggcggc ttcgttatcc aaggcggcgg attgaccgag
     6901 gacttggcac aaaaggcaag cgataaggcc gttgccaacg aatccggcaa cggcttgaaa
     6961 aacactgtcg gcaccatcgc catggcgcgg acggccgatc cggattccgc caccagccaa
     7021 ttctttatca atctggcgga caatgattca ctcaactaca aaaacggaca atacggctat
     7081 accgttttcg gcagggtcga aagcggcatg aacaccgttt ccaaaatcgc ccgcgtcaaa
     7141 accgccacgc gcggctttta tcaaaacgta cccgtacagc ccgtcaaaat ccgtcgcgtt
     7201 gttgtcgggc agtaacacgc agacagacgt tcagacggcg tcgcccgttt cccaaaaaac
     7261 gccgtttaaa ttaaaaaaat attttaaaac agacagttga tattgacaaa ttcaaaccga
     7321 ggattttaaa atgc
//
LOCUS       DAWGJV010000106         7303 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWGJV010000106 DAWGJV010000000
VERSION     DAWGJV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.106"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            150..1088
                     /locus_tag="WHQ94_001538"
     CDS             150..1088
                     /locus_tag="WHQ94_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ2128796.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1217..1510
                     /gene="hfq"
                     /locus_tag="WHQ94_001539"
     CDS             1217..1510
                     /gene="hfq"
                     /locus_tag="WHQ94_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ2128797.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            1594..2160
                     /locus_tag="WHQ94_001540"
     CDS             1594..2160
                     /locus_tag="WHQ94_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2128798.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            2214..2588
                     /locus_tag="WHQ94_001541"
     CDS             2214..2588
                     /locus_tag="WHQ94_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ2128799.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            2598..3092
                     /gene="folK"
                     /locus_tag="WHQ94_001542"
     CDS             2598..3092
                     /gene="folK"
                     /locus_tag="WHQ94_001542"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ2128800.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(3217..3696)
                     /locus_tag="WHQ94_001543"
     CDS             complement(3217..3696)
                     /locus_tag="WHQ94_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ2128801.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(3915..4652)
                     /gene="ubiE"
                     /locus_tag="WHQ94_001544"
     CDS             complement(3915..4652)
                     /gene="ubiE"
                     /locus_tag="WHQ94_001544"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ2128802.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(4695..5063)
                     /locus_tag="WHQ94_001545"
     CDS             complement(4695..5063)
                     /locus_tag="WHQ94_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ2128803.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKIVFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            5183..6802
                     /gene="mnmC"
                     /locus_tag="WHQ94_001546"
     CDS             5183..6802
                     /gene="mnmC"
                     /locus_tag="WHQ94_001546"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ2128804.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         1949 a         2273 c         1471 g         1610 t
ORIGIN      
        1 ccgatttaaa aacagaatgt tataaaaccc aatccccaaa acacaacaag accgcccgcc
       61 tacgggcagt ctcctgtcag acgacatact ttacagatgg ctgttttttc aacaaaataa
      121 cgccaatact caaaaatatg gaatcaaaaa tgtccatcca tactctgaaa cgcctgccct
      181 catcgctgct gctcggtctc tgcctttccc tgccgtcagc ccaccttttt gccgacaacg
      241 acattttagg gcaattttta gaacagaaca tgcttacctc ctccgatccg atagaaatat
      301 tcgccgaaag cacgatacac cccaccaaca cccaagccat tacaggcggt ctgattctct
      361 cctcacaatc tgccctggtc gtcaacaaca aaaccggaca gatactgtat cagaaaaacg
      421 ccgacaggat tatgcccatc gcctccattt ccaaactgat gagcgcgatg gtcgttttgg
      481 atgcaaactt ggacatgaac gaaaccgtta ccattacgcc cgacgaaatc gaccgcatca
      541 aagggaccgg cagccgtctt gccataggta cggcacttac acgcaaagaa ctgctgcacc
      601 tgagcctgat gagcagcgaa aaccgcgcca cccatgcatt gggcagaacc taccccggcg
      661 gcatgggcgc atttgtcgcc gccatgaacc gcaaagccca aagcctcggt atgtacggca
      721 gccgctttta cgaaccgacc ggactcaact tccaaaacgt ttctaccgcc aaagacttga
      781 gccttatggt caacgccgcc gcccaatatc cgcaaatccg caccaactcg acttccaact
      841 acgcctcggt acagaccaaa aacgggcagc agaactacaa aaactccaat gccctggtca
      901 gagaaggcat gtggaacatc gaattgcaga aaaccggcta catacgcgaa gcaggcaggt
      961 ctatggttgt caaagccaac attcaaaatc aacccgttac catcgtattg ctgaactcgc
     1021 ccacatccgc cacacgcgtc aacgacgccc gcaaaatcga atcgtggatg ctgcagcaac
     1081 gctcctgaca tacaaatgcc cggcggaaaa ccgcccaaac acaaaaaaaa ttggtaattt
     1141 ttcctattca aggctacaat tcgacacgca cagggcatct gcccgatttc acaatcataa
     1201 tagcggagtt aaaaatatga cagctaaagg acaaatgttg caagatccct tcctgaacgc
     1261 cctgcgtaaa gagcacgttc cggtttcgat ttacctggtc aacggcatca aactgcaagg
     1321 tcaggttgaa tctttcgacc aatacgtcgt tcttctgaga aacacttccg tcacccaaat
     1381 ggtttacaaa cacgccattt ccaccatcgt accggcacgc tccgtcaacc tacaacatga
     1441 aaacagaccc caagccgcac cggcttcgac cctcgtccaa gtggaaaccg tccagcagcc
     1501 tgccgaataa tccgcacgaa gcatgacgtg tcatatcttt caatacctta ccgacatcgg
     1561 taaggtattt ttattttcag acagcattta aaaatgttat tgcaaaacat ccttccattc
     1621 gcccattgcc ttttgcggaa ggcacttccc gaaggcggca atgcgctgga cggcaccgcc
     1681 ggcaacggac acgacaccct tttcctcgca caaaccgcag gcatccgggg gaaagtgtgg
     1741 gcattcgaca tccagccgca agccctgaac aacacccgat gccgtctgca ggaagcaggt
     1801 tacagcaatg tacggttcat cttggacgga catgaaaacc tgaagcaata tattccaaag
     1861 ccgctggatg cagccatttt caatttcggc tggctgcccg gcggggacaa aagccttacc
     1921 acccgcacgg aaaccagcat tgccgccctt tctgccacct tatccctact gaaagaaaac
     1981 ggtatgctta ttgccgtcct ctatccggga cacgaaaacg gcaaacagga ggcagaagcc
     2041 gtcggacaat gggcaaaaaa cctgcctcaa gaacagtttg ccgttttacg ttacggcttt
     2101 accaaccgga aaaacagccc accctatctg ttggcatttg aaaaactgcg tcaaaaataa
     2161 ctgtttgcgg taaaataagc cgattccaac ccttattttg ataagagctg accatgcagt
     2221 atctgtttgt caaatacagc caccaaatct tcgttaccat caccattttg gtattcaaca
     2281 tccgtttttt cctactttgg aaaaatccag aaaagccctt ggcgggcttt tggaaggcac
     2341 tgccacacct taacgacacc atgctgctgt ttacgggatt gtggctgatg aaaattaccc
     2401 atttctcccc gttcaacgcg ccttggctcg gtacaaaaat cctgcttctg ctcgcctata
     2461 tcgcattggg tatgatgatg atgcgcgccc gtccgcgttc gaccaagttc tacaccgttt
     2521 acctgctcgc catgtgttgc gtcgcctgca tcgtttacct tgccaaaacc aaagtcctgc
     2581 ctttctgaaa caccgttatg aacaacagac attttgccgt catcgccctg ggcagtaatc
     2641 ttgaaaaccc tgcccaacag gtacgcgccg cattggacac gctgtcgtcc catcctgaca
     2701 tccgtcttaa acaggcttcc tcactgtata tgaccgcgcc cgtcggttac gacaatcagc
     2761 ccgattttgt caatgccgtc tgcaccgttt ccaccactct ggacggcatt gccctgcttg
     2821 ccgaactcaa ccgtatcgag gctgatttcg gacgcgaacg cagcttccgc aacgcgccgc
     2881 gcacattgga tttggacatt atcgactttg acggcatctc cagcgacgac acccgactca
     2941 ccctgccgca tccgcgcgcg cacgaacgca gtttcgtcat ccgccctttg gcagaaatcc
     3001 tccctgattt tgttttagga aaacacggaa aggttgccga attgtcaaaa cggctgggca
     3061 atcaaggtat ccgtctttta cccgacaggt aattccgcac gcggatgccg tctgaaagcc
     3121 tttcagacgg catttttcct ttgccgccaa cacgcgtgca aaaaaatcgc cccttggaaa
     3181 aggggcgcaa aaggaacaca aaccactacc aaaactttaa atctgaaaca ctgcctgccg
     3241 catactgtat ccgacaggat ataaagccct cactaaatcg tttcgagaaa tccaaacttc
     3301 ttcatcgccg acagaaaatc tgcctttctc cggtaccagc tccaacagaa acggttgaac
     3361 cgccgtatgc aacttgtcct tacaccgccc cagctttcta gacaaggcgg acagggcgcg
     3421 tttgtaggca ttatccttgc agtcaagctc ccgcgcactg ccgacccagc tcaaacgaag
     3481 gttgcggctt tcatcctcaa tcagcaaaat gcctgttttg acttccccca cctcgggctc
     3541 gcatgaaagc gcaatataat atttgccgtc gatgttgaca tacgccgctt catgcacctg
     3601 acagttgcag ccggaagtgg caagcagcga ataaacagaa tcccgatgca gtttcaaaat
     3661 atcaagggaa atctgtttaa aaatactttc cggcattttc cctcctttcg tctgctgcgt
     3721 tgtttactgg attgactgca ttatacagac atctcccaat cttgcaaatg ataattgttc
     3781 ctatttttta aaaaatttat aaattattga tagatatgtg aattaatttt aagatttaaa
     3841 atctctttct caaaaaaaca caaaaatgcc gtctgaaacg tgttcagacg gcattggctg
     3901 cacagccagt ccgtttaaaa tttcacgccc ttatgcagcg cgacgatgcc cgcactcata
     3961 ttgtgataat ccacgctgtc aaagcccgca tccaacatca tctgtttcaa agtttcctga
     4021 tcggggtgca tacggatgga ttcggcaaga tattgataac tctccgcatc tttggcaatc
     4081 agcctgccca tgaccggcag cagcttgaac gaatagaaat catacgcgcc ttccaaaggt
     4141 ttgtagattt tggaaaactc caacaccagc aacgtgccgc ccggtttcaa aacacggtac
     4201 atctctttca gcgcggcatc tttatgcgtc atgttccgca agccgaacgc cacggaaacc
     4261 aagttgaaat aattgtcggg gaaaggcagt ttttccgcat cggcaagcga cacgggcaaa
     4321 atcatgcctt cgttcaacag acggtcgcgc ccgacggtca gcatagaaga attaatatcg
     4381 gtcagccaaa cctcgccttc cttgccgacg cgtttcgccc accctcggga caaatcgccc
     4441 gtaccgcccg caatgtccaa cactttatcg ccttttttca ggtgtgccgt gttgatggtg
     4501 aaatgcttcc acaccctgtg cagccctgcc gacatgacat cgttcataat gtcgtagttt
     4561 ttggcgacgg agtggaacac ttccgccact tttccggctt tctcatcttc gttgaccgtg
     4621 ctgaacccga aatgcgtttt ctgtccgccc atattctaac cacctccctg atgccgccct
     4681 acggcatccc aaacttaaca atctgattgc ttttcaaaac gggaagcacc cgccgccgcc
     4741 aatttgtcca aatattcctg ccacatcgca tcgtatccgt atgccagtct gtgcagatac
     4801 gcccaatcgt aaagaccgct gtcgtgcccg tccgaaaaaa cgattttcag cgcgtactgt
     4861 ccgacaggct gcaaatccgt aatttgcaca tccgccttgc cggtctgcaa aacatcctgt
     4921 cccacgccgt gtccgcgcac ttcggcactc ggcgaataga cgcgtaaaaa ttcggcaggc
     4981 agatttttag gttcgtcccc ataagtcaaa accaacacgg tacggttttt caacaaacgg
     5041 atttcaacag gaatattatt caaaaaacac ctcggttcgg catacaatat ccggcaggcg
     5101 tacgcgtgcc tttaaacggc gcgtatttta acagctttat cccgataccg atattcaaac
     5161 ccacacttta aacggcattg atatggacaa tctcgtatgg gacggcattc ccgacatccg
     5221 cacactcgac caaaccatcc gcaaacacgc acacccgctc aacctgattg tctgcctccc
     5281 cgataatcag attcccgatt ttcaaaccgc acaagatgct tcggacgcgg aatgccgtct
     5341 gaagcaccgt ttggatcagg caacgcagtg cctccagttc gacagcatca acctcatcga
     5401 acacatcctg cccgatgtcc gcttctggct ggttccccct tcacgcaccc gccgcctgca
     5461 cgaacacttc caccacattt cctggcagac cgaagccatc ccgcaaaccg aaagtaagcc
     5521 cgacaaaccc tggtttgcac ttccacaaac atccgaacgg caaaaaccgg aacacatcct
     5581 cgttatcgga gcgggcatat ccggcgcggc aaccgcccac gccttagcat catacggcat
     5641 ttccgttacc gtattggaag cccgaaaagc cgcccaagcc gccagcggca accgccaagg
     5701 gctgctctac gccaaaatct cgccgcacga caccgaacaa accgaactgc tgcttgccgg
     5761 ctacggctac accaaacgcc tgctcggaca tatcctgccc gaatccgaaa cctggggcgg
     5821 caacggcatc atccacctca attacagccg caccgaacaa caacgcaatc acgaattggg
     5881 tttgcaaaaa caccataacc acctctaccg cagcatcaca tctgcagaag ccgaaaaaat
     5941 cgccggtatc ccactgtccg tcccatacga ccacccttca tgcggactct actggcaaca
     6001 cggcgtatgg ctcaatccac ccgcattcgt ccgcaccctc ctcaaccatc cgctcattgg
     6061 actacacgaa gacacaccgt taaccgacat ttcccacgac ggggaaaagt ggattgcaag
     6121 cacgccaaac ggcatatttt ccgccacaca catcatctac tgcaccggcg cgcacagccc
     6181 ccgcctgccc gaaaccaacc tcgccgccct gcctctcagg caaatacgcg gacaaaccgg
     6241 cctcacaccg tccaccccgt tttccgaaca actgcgttgc gccgtttcag gcgaaagcta
     6301 catcagcccg tcgtggcacg gactgcactg ctacggcgcg agttttattc ccaacagcag
     6361 ccataccgga tggaacgaag ccgaagaagc ctcaaaccgc caagcattgg cacaccttaa
     6421 ccccgccctt tccgaatcat tgtttgccgc caacccaaac ccccaaaaac accaagggca
     6481 cgccgccata cgctgcgaca gccccgacca ccttccccta gtcggcgcac tcggcgacat
     6541 tgccgccatg cggcagacct acaccaaact cgcgctggac aaaaactacc gcatcgacac
     6601 cccatgccca tacctgccta atgcctacgt caacaccgcg cacggcaccc gcggactcgc
     6661 caccgccccc atctgcgccg ccgccattgc agcccaaatc ctaggcttgc cccatcccct
     6721 ctcaaaacgc ctgcgccacg ccctacaccc caaccgcacc atcatccgcg ccatcgtcag
     6781 aaggaaggat ctaacccctt aagcctttgc cgcatgccgt cccgaccaca gaaaatcata
     6841 ttcaaataaa cataccaatc ctgacggtaa aatatcgaaa acacccataa cgttttcttc
     6901 ccaaaataag gagtaaggaa tgcctaacaa attgcctttg ctgtctaaga tgaatgttcc
     6961 gtaacattcg aagacttcct taaccctgcc agccgtttcg gtttctgtac cccctgttag
     7021 ataaggaagg aatgaaaaag ccctgttgat tgtatgaagt aaagggcgtt aatttttgtt
     7081 aatcttccct tctcaggaac tcaatatata aggatgttaa tttttatttc ccttctcgag
     7141 gactcaatat agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag
     7201 aacgattctc taaggtgctg aggcaccaag tgaatcggtt ccgtactatt tgtactgtct
     7261 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tag
//
LOCUS       DAWGJV010000107         7284 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWGJV010000107 DAWGJV010000000
VERSION     DAWGJV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.107"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..532
                     /locus_tag="WHQ94_001547"
     CDS             <1..532
                     /locus_tag="WHQ94_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ2128805.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVKIIKNASDIQEFSAQDDGLIIIDNVNGLHAGT
                     DYTDKNRHYIRARITV"
     gene            542..1309
                     /locus_tag="WHQ94_001548"
     CDS             542..1309
                     /locus_tag="WHQ94_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2128806.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            1509..1886
                     /locus_tag="WHQ94_001549"
     CDS             1509..1886
                     /locus_tag="WHQ94_001549"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ2128807.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            1968..2777
                     /locus_tag="WHQ94_001550"
     CDS             1968..2777
                     /locus_tag="WHQ94_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ2128808.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            2770..3192
                     /locus_tag="WHQ94_001551"
     CDS             2770..3192
                     /locus_tag="WHQ94_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128809.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            3241..4383
                     /locus_tag="WHQ94_001552"
     CDS             3241..4383
                     /locus_tag="WHQ94_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ2128810.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(4441..5691)
                     /locus_tag="WHQ94_001553"
     CDS             complement(4441..5691)
                     /locus_tag="WHQ94_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2128811.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVAADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     PNKTRRGSPEADLTQNTTPQPGRGRNGRRPVIMIDPGHGGEDPGAVSSGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(5688..6149)
                     /gene="tsaE"
                     /locus_tag="WHQ94_001554"
     CDS             complement(5688..6149)
                     /gene="tsaE"
                     /locus_tag="WHQ94_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ2128812.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILHGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLAAHTERGRESLPL"
     gene            6336..7148
                     /locus_tag="WHQ94_001555"
     CDS             6336..7148
                     /locus_tag="WHQ94_001555"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ2128813.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYCFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT         1733 a         1813 c         2015 g         1723 t
ORIGIN      
        1 tgatataact ttctcggaag atgttggaga atgtccactt catagtgatt catcttttag
       61 tgaaaacccg gaaagttatt tggttatgta tgtagtaaaa tcagccaatg atggaggtaa
      121 ttccctattt ttaagttcat cagatattgt caatcagtta tctaaaacag aaaccggtaa
      181 aaaacactta aaaacattaa cgggcaattt atatccattt aaaacaccag catcatttga
      241 taaaaaacaa ggtgtgagat ggggtaatat cttatcggtc aatactcaaa tgattagatt
      301 tagaagtgat tgtatctata aaggtattga agaaaataga aataaagtat caaaggaaat
      361 ggtacttgca cttgattatc ttgtaaagat tataaaaaat gcgagtgata ttcaagaatt
      421 ttctgcacaa gatgatggtt tgattattat tgacaatgtc aatggcttgc atgccggaac
      481 tgattatacg gataaaaaca ggcattatat tagagcaaga attactgtat aaaggacggt
      541 tatgcaagaa ataatgcaat ctatcgtttt tgttgctgcc gcaatactgc acggaattac
      601 aggcatggga tttccgatgc tcggtacaac cgcattggct tttaacatgc cattgtctaa
      661 ggttgttgcc ttggtggcat taccaagcct gttaatgagc ttgttggttc tatgcagcaa
      721 taacaaaaaa ggtttttggc aagagattgt ttattattta aaaacctata aattgcttgc
      781 tatcggcagc gtcgttggca gcatttttgg ggtgaagttg cttttgatac ttccagtgtc
      841 ttggctgctt ttactgatgg caatcattac attgtattat tctgtcaatg gtattttaaa
      901 tgtatgtgca aaagcaaaaa atattcaagt agttgccaat aataagaata tggttctttt
      961 tgggtttttg gcaggcatca tcggcggttc aaccaatgcc atgtctccca tattgttaat
     1021 atttttgctt agcgaaacag aaaataaaaa tcgtatcgta aaatcaagca atctatgcta
     1081 tcttttggcg aaaattgttc aaatatatat gctaagagac cagtattggt tattaaataa
     1141 gagtgaatac ggtttaatat ttttactgtc cgtattgtct gttattggat tgtatgttgg
     1201 aattcggtta aggactaaga ttagcccaaa tttttttaaa atgttaattt ttattgtttt
     1261 attggtattg gctctgaaaa tcgggcattc gggtttaatc aaactttaat tcattattaa
     1321 atgccttaac tccttattaa ataattggca cgatgtttta gaatttcaaa tgcaaaaggt
     1381 tacagtgaaa attgttaccg acaaaacccc aaaagtggat attcacgcca ttttaacgcc
     1441 ccaagaaatt gacggcattc atcatcacat tcatcactac ccgcaaccaa gggcgaagga
     1501 gcgcaaatat gatttacggc atcggcacag acattgtttc cctcaagcgc atcatccgct
     1561 taaacaaaaa attcggacag gcgtttgccg ggcgcatcct cactccggaa gagctgcttg
     1621 aatttccgca agcgggcaaa cccgtcaact acctcgccaa acgctttgcc gccaaagaag
     1681 ccttcgccaa agccgtcggc acgggcatac gcggcgcggt ttccttccgc aacatcggca
     1741 tcgggcatga cgcattgggc aagcccgaat ttttctacgg ccccgccctg tccaaatggc
     1801 tggaggaaca aggcatcagc cgcgtcagcc tcagcatgag cgacgaagaa gacaccgtat
     1861 tggcgtttgt cgttgccgaa aaataatgcc gtctgaaatg cggcaaaccc gttgacggca
     1921 ttgctcgtcc ctcatttgca ctccgaccga ccaaccgcgt acccgccatg attcaagaca
     1981 cccgacccct tatccgcgtc gttgccggca tcctgctcga ttcagacggc aactacctgc
     2041 tcagctcgcg ccccgaaggc aaaccctatg ccggatattg ggaatttgcc ggcggcaagg
     2101 tcgaagcggg cgaaaccgac ttccaagcct tgcaacgcga gtttgaagaa gaactcggca
     2161 tccgcatcct tgccgccacg ccttggctga ccaaaatcca ttcctacgaa cacgcccgcg
     2221 tctgcctgaa attcctatgg gtcaaccccg gccaatggac gggcgaaccg caatcccgcg
     2281 aagggcagga atggtcttgg cagaaggcgg gtgattttac cgttgccccc atgctgcccg
     2341 ccaacggcgc gcttttgcgt tcgctgtccg tcccgcgccg tttgtacggc agcctgaaaa
     2401 cgggtttgca cggggagaac agtatgggcg cgtaccgcgt cctgcctttg ggttcggcag
     2461 agggaagcgg tgcgaacgtt ttgatggagg cggcgcaatg gcaggacaga cccgaacacg
     2521 ccgacagcgt gtggatgatg gtgcagaccc gcgaacaatg gcggcaggcg caggaaaagg
     2581 gcgcggatgt tgtcgtgtgg cgcgtgtgca atgatgttca ggcacaagag gcggcagaag
     2641 ccctgcggca gggcgtatcc gtgccgctcg tacttgcagc aaacggacag acggttgcac
     2701 gttatggaaa actatggctc ggattggggg cgcacgtggt ggtaagggat gaaacaatag
     2761 ggaagaatca tgaataaaaa ccgtaaatta ctgcttgccg cactgctgct gattgccttt
     2821 gccgccgtca agctcgtttt gttgcaatgg tggcaggcgc agcagccgca agctgtggcg
     2881 gcgcaatgcg atttgaccga gggttgcacg ctgccggacg gaagccgcgt ccgcgccgcc
     2941 gccgtttcaa ccaaaaaacc gtttgatatt tatatcgaac acgcgcccgc cggcacggaa
     3001 caggtcagca tcagcttcag tatgaaaaat atggatatgg gtttcaaccg ctatatgttc
     3061 gagcggcaac cgtcggggac ttggcaggca gtacgcatcc gcctgcccat ctgtgtcgaa
     3121 ggcaggcgcg attttacggc ggacattaca atcggcagcc ggacatttca gacggcattt
     3181 accgccgaat aaacctttca atccgccatt gccggaacat ccgtccggaa aggacacgtt
     3241 atgaatactt tatatacact tttcgccacc tgcccgcgcg gcttggaaac cgttttatcg
     3301 caagaactcg aaagcctcgg ctgtaccgat gttcaagcgt ttgacggcgg cgtttcctgc
     3361 cggggcggat tggaacaggt ttacgccgcc aacctgcact cgcgtaccgc cggccgcatc
     3421 ctgctacgcc tgaccaaagg cacataccga accgagcgcg acatttacaa acttgccaaa
     3481 aacatcaact ggttcaactg gtttaccttg cagcagacgt tcaaagtcaa aatcgaggca
     3541 aagcgtgcca acgtcaaaag cctccaattt gtcggactga ccgtcaaaga tgccgtctgc
     3601 gacgctttcc gcgacattta cgacgcacgt ccgagcgtgg acaaagccgc gcccgatgtt
     3661 cgcatccacg cctttttgga cgaacgcaat gtcgaaatct tcatcgacac ttcgggcgaa
     3721 gccctgttca aacgcggcta ccgtctcgat accggcgaag ccccgctgcg cgaaaacctt
     3781 gccgccggat tgctgctctc ggcaggctac gacggcacgc agccgtttca agacccgttt
     3841 tgcggcagcg gcacgattgc tatcgaagcc gcttggattg ccgcccgccg cgcgccgggt
     3901 atgatgcgcc gtttcggttt tgaaaaactg caaaatttcg ataaaacgct gtggtcggat
     3961 ttgcgccgcc gtgccgaagc gcaggttaaa ccggcttttg caccgatttc gggcagcgac
     4021 agcgaccgcc gcatcattca gacggcattg gccaacgcac gcagtgccgg ggtggacgac
     4081 atcgtttcct tcagcgttgc cgacgcgcaa tccgtccgtc cgaacggcgt gggcggcatt
     4141 atggtgtcca atccgcccta cggtgtgcgc cttgaggaag tccgcgcttt gcaggcactg
     4201 tatccgcagt tggggacgtg gttgaaaaaa cactatgccg gctggcaggt cggtatgttt
     4261 accggcgaca gggaaatgcc caaattcatg cgcctgtcgc ccaagcggaa aattccgctt
     4321 tataacggca acctcgactg ccgcctgttc ctgattgata tggtgcaggg atcgaaccgt
     4381 tgaggaaagt gtacaaaaaa gccgtctgaa aaatgttcag acggcattta tttttcggaa
     4441 tcaaccccgc ttcaatacgg aagtgttgat gtagcgttgg acacccgagg caatggattg
     4501 ggcgcactgc cgacggaagg attcgctgcc cagcagcttc tcttcggcag ggttggacag
     4561 gaaggcggtt tcgaccagga tagacggcat atcgggcgcg cgcaatacgg cgaaattggc
     4621 ttcgtccacc ctgcctttgt gcagatggtt gagcctgccc aattcttcaa gcaccagttt
     4681 gccgagtttg cggctgtcgc gcagtgtggc ggtttgggtc atatcgagca gggcggtatc
     4741 gacgttgcgg ttgccgctgg tcggtacgcc gccgaccgcg tcggcattgt tttgcgtctg
     4801 ttccaagaat ttggcggcgg agctggtcgc gcctttggtg ttcagcatat aaacccccgt
     4861 tccgcgcgcg gaggggctgg tgaaagcgtc ggcgtggatg gagacgaata cgtccgcccg
     4921 ccgcgcgcgc cctttggcga cacgcacgcc caatgggatg aacacgtctt cgttgcgcgt
     4981 cataaagacg ttgtaaccca aggcttccaa ttgttttttg gtttcgcggg cgatggagag
     5041 gacgacgtgt ttttcctgca aaccgcccga gctgacggcg ccgggatctt cgccgccgtg
     5101 tccggggtcg atcataatga cgggcctgcg cccgtttctg ccgcgcccgg gttggggcgt
     5161 ggtgttttgg gtgaggtcgg cttcgggaga gccgcgccgg gttttgttcg ggctgccgtt
     5221 aagcagcgcc atcatcggat cgtcggcatc catcccttgc ggatagagat cgacgacgag
     5281 gcggttttta aagccgccga cgggggggag ggaaaaaact tgcgcgtagg aaggctgttt
     5341 caaatcgatg acgaggcgga cggtggtcgg tgtgttctga cccgcgcgta tgctgcggat
     5401 aaaggggtcg tccgccgcca ctttttggga cagtccgtgc aatacggtat tgatgtttgc
     5461 gttttgtatg tcgacgacca gcctgcccgg attgtcgagc gcgaagtgct ggtatttgag
     5521 cgcggcggtg ctttccagcg tcaggcgggt gtaggtgtgc gacggccata tccgtgcggc
     5581 ggtgaattgt ggtgcgcgta ccgttttggc aacggcggat gcgatggggc ttagggcgaa
     5641 cagtgtgccg gcggtgcggc ggatgatttg tcttcgtgtc agtttggtca tagcggcagg
     5701 ctttcgcgtc ctcgttcggt atgggcggcc agcaggcatt ttctgccgcc gccgtcgtgt
     5761 gtcaatgttg cggtgatgtc ggcgggcggc gtaaattccc cgccctgttg cggccattcg
     5821 atcaggcaga cgctgtttgc ggcaaacagt tcgtcaagcc ccgcgtcttc ccattcttcg
     5881 gggaacgaga agcggtagag gtcgaaatgg tgcagggtga agcgttccag cggataagat
     5941 tcgacgatgg cgtaggtcgg gcttttgact gcgccctgat gacccaatcc gtgcaggatg
     6001 ccgcgtgtca gcgtggtttt gcccgcaccc aaatcccctt cgaggtaaat gaccagcggt
     6061 gcgtttaaac gggaagacca cgccgcaccc aaatcgagtg tggcggcttc gtcggcaagg
     6121 aatcgggaaa caggggagaa atcagacata ggggctttac aacttttatg tggcggatcg
     6181 gaggggcagg cggtcggaaa accgtgcgcc cggcggcatt gccgtacatt ataacagcca
     6241 aatccgcatc ctgctttcag acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata
     6301 cgcggattgt atgtttagga cggattggaa aaaggatgga aaatatcggc aggcagcgac
     6361 ccatcggcgt ttttgactcg ggaatcggcg gtttgaccaa tgtgcgcgca ttgatggagc
     6421 ggctgccgat ggagaacatc atttatttcg gcgacacggc gcgcgtgcct tacgggacga
     6481 aatctaaggc gaccatcgaa aatttctcga tgcagattgt cgatttttta ttggaacacg
     6541 atgtcaaggc gatggttatc gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc
     6601 gtcaaaagac gggcaatatg ccggttttgg acgtgatttc cgccggcgcg aaagccgcgc
     6661 tggcaacgac gcgcaacaat aaaatcggca ttatcgccac caataccaca gtcaacagca
     6721 atgcttatgc gcgcgccatc cacagggaca accccgacac gctcgtccgc acgcaggccg
     6781 cgccgctgct cgtccctttg gtggaagagg gctggttgga acacgaagtt acccgcctga
     6841 ccgtatgcga atacctcaaa ccattgcttg cagacggcat cgatacgctg gtgttgggct
     6901 gcacgcactt tcccttgctc aagcccttaa tcggcaggga ggcgcacaat gtcgcgttgg
     6961 ttgattctgc aattacgacg gctgaagaaa ccgcgcgcgt ccttgcgcag gagggattac
     7021 tcaataccga caacaacaat cccgactatt gtttttacgt cagcgatatt cctttgaaat
     7081 tcagaaccat cggcgagcgt tttctgggca ggacgatgga gcagattgaa atggtgtctt
     7141 tgggttaaaa cgatgacgga aagctgcccg agattacaga aacctaaaat cccgtcattc
     7201 ccgcgcaggc gggaatctag acattcaatg ctaaggcaat ttatcgggaa tgactgaaac
     7261 tcaaaaaaac tagattccca cttt
//
LOCUS       DAWGJV010000108         7122 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWGJV010000108 DAWGJV010000000
VERSION     DAWGJV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.108"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WHQ94_001556"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WHQ94_001556"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WHQ94_001557"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WHQ94_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ2128814.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WHQ94_001558"
     CDS             complement(1737..2630)
                     /locus_tag="WHQ94_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ2128815.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYFDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WHQ94_001559"
     CDS             2972..3421
                     /locus_tag="WHQ94_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ2128816.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WHQ94_001560"
     CDS             3495..3803
                     /locus_tag="WHQ94_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128817.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="WHQ94_001561"
     CDS             3919..4761
                     /locus_tag="WHQ94_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ2128818.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="WHQ94_001562"
     CDS             4751..5497
                     /locus_tag="WHQ94_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ2128819.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="WHQ94_001563"
     CDS             5519..6106
                     /locus_tag="WHQ94_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ2128820.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="WHQ94_001564"
     CDS             6112..6291
                     /locus_tag="WHQ94_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ2128821.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="WHQ94_001565"
     CDS             6532..6972
                     /locus_tag="WHQ94_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128822.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1809 a         1897 c         1624 g         1792 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acggttcaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gatttcaaca tttccatcgc ggccgctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgacaac ttcttccaat cggtctttct tgccctgata atccatataa
     1561 acgcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tggaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaaccgga tggcgcgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgaa ataagagaag aaaccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atccgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgacaatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcggcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atggaggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttctga aatcaacgat caattcccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcgcgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc ctttaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgcgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgccgtcgc acgtgaggcc gattgcggca ttcatctgga tgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctgttggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga agtcgccaaa atcgccgatt atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaacctccgc ccaataccag caccgtttcg cccaagccat
     4801 acgcgggggc gaagccgcag acggtctgcc gcaagaccga ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttt
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cttcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctagaaataa tggggtcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc acactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatctg
     6361 cagttcatcc ataatacaca cttcaaaagc agcatatttc cccacacgga atgtataaat
     6421 acgcaaaata cgaaggctgc atcaatttgc catatttgct ttatttgcct tatttcacag
     6481 acgacactcc ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttac tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaacaa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgtgaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 cagttgtttg atacaaaaaa cacggaaatt tccacacaaa gctacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cggaacatta actgaaaaac aatatgaaac cgtatgcggg
     6961 aaaaaactct gattacaact tatgcacaaa cttatccata aaccttatta taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttccc cgaaaagtta
     7081 cccccaaagt tatccacatt attttttaaa ccagcttcca tc
//
LOCUS       DAWGJV010000109         7076 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWGJV010000109 DAWGJV010000000
VERSION     DAWGJV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.109"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            126..1976
                     /locus_tag="WHQ94_001566"
     CDS             126..1976
                     /locus_tag="WHQ94_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ2128823.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2265..2477
                     /locus_tag="WHQ94_001567"
     CDS             2265..2477
                     /locus_tag="WHQ94_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ2128824.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2609..3055
                     /locus_tag="WHQ94_001568"
     CDS             2609..3055
                     /locus_tag="WHQ94_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ2128825.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3133..3375)
                     /locus_tag="WHQ94_001569"
     CDS             complement(3133..3375)
                     /locus_tag="WHQ94_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128826.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3380..3772)
                     /locus_tag="WHQ94_001570"
     CDS             complement(3380..3772)
                     /locus_tag="WHQ94_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ2128827.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3844..4449)
                     /locus_tag="WHQ94_001571"
     CDS             complement(3844..4449)
                     /locus_tag="WHQ94_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ2128828.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4551..6227)
                     /locus_tag="WHQ94_001572"
     CDS             complement(4551..6227)
                     /locus_tag="WHQ94_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ2128829.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6373..7002)
                     /locus_tag="WHQ94_001573"
     CDS             complement(6373..7002)
                     /locus_tag="WHQ94_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ2128830.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         1831 a         1949 c         1893 g         1403 t
ORIGIN      
        1 attatgtcgg aaacattcca aaggcggtgc agtttcggca tataattcgg gcaaacgcct
       61 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
      121 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcgacc ctagtgcttg
      181 ccgcgtgttc ttcgacaaac acactgccag ccggcaagac cccggcagac aatatagaaa
      241 ctgccgacct ttcggcaagc gttcccaccc gccctgccga accggaagga aaaacgctgg
      301 cagattacgg cggctacccg tccgcactgg atgcagtgaa acagaaaaac gatgccgccg
      361 tcgccgccta tttggaaaac gccggcgaca gtgcgatggc ggaaaatgtc cgcaacgagt
      421 ggctgaagtc tttgggcgca cgcaaacagt ggacgctgtt tgcacaggaa tatgccaaac
      481 tcgaaccggc agggcgcgcc caagaagtcg aatgctacgc cgattcgagc cgcaacgact
      541 atacgcgtgc cgccgaactg gtcaaaaata cgggcaaact gccttcgggc tgcaccaaac
      601 tgttggaaca ggcagccgca tccggcttgt tggacggcaa cgacgcctgg aggcgcgtgc
      661 gcggactgct ggccggccgc caaaccacag acgcacgcaa ccttgccgcc gcattgggca
      721 gcccgtttga cggcggtaca caaggttcgc gcgaatatgc cctgttgaac gtcatcggca
      781 aagaagcacg caaatcgccg aatgccgccg ccctgctgtc cgaaatggaa agcggtttaa
      841 gcctcgaaca acgcagtttc gcgtggggcg tattggggca ttatcagtcg caaaacctca
      901 atgtgcctgc cgccttggac tattacggca aggttgccga ccgccgccaa ctgaccgacg
      961 accaaatcga gtggtacgcc cgcgccgcct tgcgcgcccg acgttgggac gagctggcct
     1021 ccgttatctc gcatatgccc gaaaaactgc aaaaaagccc gacctggctc tactggctgg
     1081 cacgcagccg cgccgcaacg ggcaacacgc aagaggcgga aaaactttac aaacaggcgg
     1141 cagcgacggg caggaatttt tatgcggtgc tggcagggga agagttgggg cgcaaaatcg
     1201 atacgcgcaa caatgttccc gatgccgacg caaacagcgt cctccgtatg gcggaagacg
     1261 gcgcgattaa gcgtgcgctg gtactgttcc aaaacagccg gaccgccggc gatgcgaaaa
     1321 tgcgccgtca ggctcaggcg gaatggcgtt ttgccacacg cgggttcgac gaagacaagc
     1381 tgctgaccgc cgcacaaacc gcgttcgacc acggttttta cgatatggcg gtcaacagcg
     1441 cggaacgcac cgaccgcaaa ctcaactaca ccttgcgcta tatttcgccg tttaaagaca
     1501 cggtaatccg ccacgcgcaa aatgttaatg tcgatccggc ttgggtttac ggcctgattc
     1561 gtcaggaaag ccgcttcgtt atgggcgcgc aatcccgcgt aggcgcgcag gggctgatgc
     1621 aggttatgcc tgccaccgcg cgcgaaatcg ccggcaaaat cggtatggat gccgcacaac
     1681 tttacaccgc cgacggcaat atccgtatgg ggacgtggta tatggcggac accaaacgcc
     1741 gcctgcaaaa caacgaagtc ctcgccaccg caggctataa cgccggtccc ggcagggcgc
     1801 gccgatggca ggcggacacg cccctcgaag gcgcggtata tgccgaaacc atcccgtttt
     1861 ccgaaacgcg cgactatgtc aaaaaagtga tggccaatgc cgcctactac gcctccctct
     1921 tcggcgcgcc gcacatcccg ctcaaacagc gtatgggcat tgtccccgcc cgctgacgta
     1981 ccgatgccgt ctgaaacccg cccggtcttt cagacggcat ttttatcccg aacggcattg
     2041 acggcgaacc ataaatataa gacaatccga aaattgtttt tcctgctttt tcaagcagct
     2101 tgacacggca caagccgacc cgttaggagg tgatgtttcc gtcacggcgc gtatcccgcc
     2161 gccgcaaggc acagcgatac ggtaaacctt caacaccgtc tgccctaccc tttccaccga
     2221 tatgatgggc agatgaaaca accgaattta ttaaaggaaa taaaatgcct gcaatccgcg
     2281 taaaagagaa tgaaccattt gaagtcgcta tgcgccgttt caaacgcgcc gtagaaaaaa
     2341 ccggcctgct gaccgagctg cgcgcccgcg aagcctacga aaaaccgact accgaacgca
     2401 aacgcaaaaa agcggcagcc gtaaaacgcc tgcaaaaacg cctgcgcagc caacaactgc
     2461 cgcccaaaat gtactaaacg ttcaagtaca gattacaggt cagccctgtg atatgaggac
     2521 acaccgcaag acctgctctg cggtgtgttt tgcttttcag acggcatcga aacccgccgt
     2581 ttccatccga catcccagcg aggacatcat gagcctgaaa atccgcctta ccgaagacat
     2641 gaaaaccgcg atgcgcgcca aagaccaagt ttccctcggc accatccgcc tcatcaacgc
     2701 cgccgtcaaa cagtttgaag tggacgaacg caccgaagcc gacgatgcca aaatcaccgc
     2761 catcctgacc aaaatggtca aacagcgaaa agacagcgcg aaaatctaca ctgaagccgg
     2821 ccgtcaggat ttggcagaca aagaaaacgc cgaaatcgag gtactgcacc gctaccttcc
     2881 ccaaatgctc tccgccgggg aaatccgtac tgcggtcgat gccgccgttg ccgaaaccgg
     2941 cgcggcaggt atggcggata tgggcaaagt gatgggcgta ttgaaaaccc gcctcgccgg
     3001 caaagccgat atgggcgaag tcaacaaaat cctgaaagcc gtgctgaccg cctgattgcc
     3061 cgaatatcgg acaaaatgcc gtctgaagcc cgtatcgcag gttcagacgg cattttcatg
     3121 gtttgatgat atttataaaa attttgcaac acgctcatgc acagcaactg ccgctgctcg
     3181 ttggcgttgc cgctctatcg cttggttagc tgcaccataa ttgcactcta atgctgatgc
     3241 aataacgtcc caatatcgaa tcggcagggg caacacggtt tcgatacacc gaaacgggtt
     3301 ttgccgataa acggattccg tttgcgcccc atcggacaaa atgccgtctg aaacacgatt
     3361 ccgttcagac ggcatagatt tatttgacca atttcaagcc ttttttggcg ggccggggcg
     3421 cggtttcggc agaggtgttt tcaggcggcg tgtcggggcg atacgcttcc aattcaaacc
     3481 ccataccttc gccggtctcc cgtgcgaaaa ggctgaggac gtgtccgaca ggtatccata
     3541 tgtcgtgcgc ctgtccgccg aagcgggcgg aaaagctgat ccaatcgttg tcgatttgaa
     3601 ggttttgcgt ggcggtcgcg ccgatgttga gcataatttc gttgtcgcgg acgtactgca
     3661 tggggacacg cgtgtgttcg ttgacccaga caagaatgtg cggcgtgagg ctgttatcgc
     3721 tgcaccattc gcaaagtgcg cggaggatgt agggtttggt ggaagtgggc ataatgggtt
     3781 ccgtgtggtg tgcctgaatg tgaaaatgcc tacaaaacgg cgggttttgc aagcattccg
     3841 ggcttatttg cgcatggctt tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg
     3901 gaagatgcgc tcggcgtatt tgagcagcgg cgcggcactt ttgcccagtt tgacatcgta
     3961 gtggtcgagc cgccacagca gcggagcaag ggcgacatca atcatagaaa aatcttcgcc
     4021 gaggatgtat ttgcttttgc tgaacgaagg ggcaagcatg gtcagaccgt tgccgatggc
     4081 ttcgcgcgct tttgcctgtt ccttgttggt ggcggcgggg ttttctaaca cttggacgtg
     4141 gttgaacaat tctttttcca tacggtacag caccagccgg ccccggccgc gcataacggg
     4201 atcgccgggc ataagctgcg gatgagggaa gcgttcgtcg atgtattcgt tgatgatgtt
     4261 ggactcgtgc agcaccaaat cgcgctcgac cagcacggga acttggttat acggattcat
     4321 gacggcgagg tcttcgggtt tgttgtaaat atcgacgtct ttgatttcaa aatccatacc
     4381 tttttcgtac aaaacgaagc ggcagcggtg gctgaagggg caggtaatgc cggaatagag
     4441 ggtcatcata ataattgtcg ctcctgtgtg atgcctgcaa aacggctgat ttataaaggt
     4501 ttaatcgcgc aattatacgc gatttccggc acttaatcca gaaattcggc tcaatctgtt
     4561 gtttttaata tattttcccc gattttccgt atcagtgcga acttactgtc tttgttgcgc
     4621 gggcgcgcac cctgccagac cgtctgccag ccttgcgacg catccgcatt ttggggcaag
     4681 cggacgatac ggtagcggca ttgtacatcg ccgacgcggt gcggcaatgt gccgtactgc
     4741 gtccaaacaa tccgcgtgtg caggtcgccg ccgcctatgt cgatacactc gatgccgtct
     4801 gaaagctccc gttttaattc cggggaaagc gatgcctcca tactccggac gaccggcgcg
     4861 tggcttttcg ccgcgtccag ccacggcagg aacagcgtca tcagcaaagc ccaggtcagg
     4921 gtaacgcctg ccgcccagtt ggtaaccgcc tgcctgccgc gtatgttttt ccgggtaatc
     4981 gcccacagcc acaagggtgt gaacagtacg gcaaccgcca tcggaatggg atcgatatca
     5041 ggaacataat acgggctgaa ataggcggcg cgttcggcaa gcttggcggg ccagccgtaa
     5101 ttcatggcga aaaagcccgt ccacaggaac acggcaaaca gtccgaacgc cataatgccg
     5161 aaccagttga caaacgccgc cgcgccgcgc ctcaggctgt ccagttgcgc cgcgccgaac
     5221 agggcaagcg gcggaagcag ccagacgagg ttatcctgaa aacgctgcgg attgacggca
     5281 agcagcacca aaacggcaag catccagacg acgcccaaaa tcccccagtc ggtcgaaaac
     5341 aggcgcgtgc ggcaaaccgt ccaaaccgcc agcggcagcg cgggcaatgc aaaccaaagc
     5401 aggtttttca gatagtaaaa caaactgaat gccgtctgaa cgtgccgcac gccgccgaac
     5461 gtaccgaaaa cgtgatcgtc gagccattgc gcgaacagcg cgggctgcgt ttttgccaag
     5521 agcagcgggt aaacggtcat aagcggcagg gcaaaggcaa gtgaggcgac tgccgtcaac
     5581 atcaaacgcc tgctttgcca cggacggaaa aacatcagta cgggcaaggg cagcatcagg
     5641 gcaaatgctg ccggataagc tgctgccaac gacatcagcg tccagcccgt accgagcaga
     5701 aaagaggcgg caatcacgcg ccggcgagcc aaagaataac cgtgcagcac cagtccggcg
     5761 gcggcaaagg cggcggcagc ggggttgaga aagtgtacgg tcggaatcag cccgatacag
     5821 ccgatgagaa tcaggacgac gctgcgcccg tggtgtctgc ccaaaaagtt gaagccagca
     5881 aagccgcagg aagtcagccc gacaacggcg aaaaacacgc ctgcaaagcg tgcggcatca
     5941 tacgggtcgg ctgcccacgg cgacagcaaa tgtttgaacg cggcggcaac ccaaagatac
     6001 acgggtggta tgccgaaatc ggcatgcccg aacagatggg caaccaaggg ggtggggctg
     6061 cctgccagtg cttcgacggc ggtatagacg gcaggttcgg caggattcca caaatcgtgg
     6121 gaaaacacgc cgggccacaa ccaggcaaac gccatcaaca gcagcagcca cggcttttcg
     6181 tgggttttgg cgggcgggcg ggcatcgggc ggagtgtagg tcagcataag cgtgagaaaa
     6241 aatggacgga ttgccaagtg tagcaaatat tcgcacaaag gtcgtgcaga gactgcttca
     6301 gacggcatca gacacaaaaa gaccggcaac aaaaaagact gcacatggca gtctttgcag
     6361 atactatctt tttcataata ttttttcctg gcccgacaca gtccatagca tatcaaaact
     6421 gttgttttca atcaggatat atacccccaa tcctaaataa acaacagcaa caaaccatct
     6481 gctatatttt tccaaagttt ctccgacaga agggacttgt gccaattttt gggcagaaaa
     6541 aaccaagaga taaatcatga ctagaaaggt aagtaaagcc actatcaaat tcgctgaatt
     6601 taaggtagta aaatatggga caaagacacc aatattgtca gcaccacaac ttgcaaaagt
     6661 aatcatagcg actagaaaaa tcaggttttt attatctttg cgcaaaccct ctttggcaat
     6721 agcctctcca tcagaatctc ctaacagcaa aactttgatg cctaggaaaa ttggaatcaa
     6781 gccgagcaaa cctaaaatct ctttactagg aatataatct aagacaaatg caaaaagcaa
     6841 acttagcaat atcagactaa cagagcctag aaattgtcct aaatagatgt taatgatgtc
     6901 ttttctgctt tttcttttgg caaaaaataa cattaggata ataagtaagt ctacggctgt
     6961 cccagaatac aggattattg aagtaacgac attttgaatc ataaaacatc tcattcaaat
     7021 atatttttaa atgtattcaa acattaaacc ttgtagatgt caacttcaac cccgtc
//
LOCUS       DAWGJV010000110         7002 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWGJV010000110 DAWGJV010000000
VERSION     DAWGJV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.110"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(72..1493)
                     /locus_tag="WHQ94_001574"
     CDS             complement(72..1493)
                     /locus_tag="WHQ94_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ2128831.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1851..2210)
                     /locus_tag="WHQ94_001575"
     CDS             complement(1851..2210)
                     /locus_tag="WHQ94_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ2128832.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2207..2812)
                     /locus_tag="WHQ94_001576"
     CDS             complement(2207..2812)
                     /locus_tag="WHQ94_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ2128833.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2859..3341)
                     /locus_tag="WHQ94_001577"
     CDS             complement(2859..3341)
                     /locus_tag="WHQ94_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ2128834.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3545..3642
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3852..4952
                     /gene="gcvT"
                     /locus_tag="WHQ94_001578"
     CDS             3852..4952
                     /gene="gcvT"
                     /locus_tag="WHQ94_001578"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ2128835.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAGVFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDNTSPLEAGMGWTVDLKDESRDFVGKAALVA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5114..5500
                     /gene="gcvH"
                     /locus_tag="WHQ94_001579"
     CDS             5114..5500
                     /gene="gcvH"
                     /locus_tag="WHQ94_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ2128836.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            5652..6899
                     /locus_tag="WHQ94_001580"
     CDS             5652..6899
                     /locus_tag="WHQ94_001580"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ2128837.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSKAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1776 a         1684 c         1976 g         1566 t
ORIGIN      
        1 tcggaagggg gatattgatt tgattatgcc ggaatttaaa atgccgtctg aatgttcaga
       61 cggcatagcg tttacagcag tttgaaaacg aaaaagataa gggtatgtac gatgaagacg
      121 ggtgtcagga aggcgaccga ccacatcata tagccgaaga aagtcggcat cggtacgccg
      181 cgctgttcgg caatggcctt gaccatgaag ttcggtgcgt tgccgatgta ggtcagtgcg
      241 cccatgaata ccgaacccat agaaaccgcc agcagcgaat gaaacagggt acccgtcatc
      301 aaggcttggg catcgccgcc cgccatattg aaaaaaacga gataagtggg cgcgttatcc
      361 aagaatgccg acaatatgcc gctcatccaa aaatacatca cattaatcgg atgacctgcc
      421 gtatcgtgaa ccagcgatac caccccgccc agcgcgcctg cctcgcctgc tttcagaatg
      481 ctcaggacgg gaaagatggt gatgaagatg ccgaggaaga gtttgcccac ttcggcgatg
      541 ggttcaaagt tgaattcgtt gcctgcgcgg acttgtttgg gcgtgattgc catagatacg
      601 gcggtcaatg caatcaggat gacatcgcgg acgaggtttt gcagggcgta acggctgccg
      661 aggatttcaa atcccgggtg ttcgggtttc caaaggccgg acattagaac cgcgccgacc
      721 acgcccgaaa gcaggaggaa gttccatttg ccgaagatgg cgattttttc gggtttttcc
      781 tgttgtgccg gcgtatcttg tgcaatgctt tcctgtttga agaaacggtt gtcgatgaaa
      841 tagaaggcgg tcaacaggac agcggtgctg atcaggacgg gggcgaacat atgtttgacc
      901 gtccacatga aatctacgcc tttgaggaag ccgaggaaga gtggggggtc gcccaaaggg
      961 gtcagaccgc cgccgatgtt tgcaaccagg aaaatgaaga agatgacgat gtgcacgcgg
     1021 cgggtacggt tttggttggc tttcagcagc ggacgaatca tcagcattgc tgcgccggtc
     1081 gttcccatga tagaggcaag tgccgtaccg acggcaagca gggcggtgtt gagcttgggt
     1141 gtgccgttca agtcgcccca aaccaaaatg ccgcctgaaa tggtgtacag ggcaagcagc
     1201 agcaggatga aggggatgta ttcttcaacg agtgcgtgtg cgacggtatg gataccggcg
     1261 gacgcgccaa aaaccaaact gaacgggatg aggaagagca atgtccaaaa ggcggtgatt
     1321 ttgccgtaat ggtgatgcca ggtatgcgaa aaaaacaagg gacccaatgc gatagacagc
     1381 aaaatcaggg caaagggcag gctccacagc aggtttaggt ttgcgccgtc caaatctgcg
     1441 gcgtaaaccg atgcggggaa aagcattagt gaaaacaggg gtaggtggcg catcgtgttt
     1501 cctcgattca agcactgcct tgcgcggcgc gtgggagtga tacaggcacc gtgccgcccg
     1561 gacataggcg gactgtgctg cctgtttgtt tttgaaaaaa gttttccgat tgattgtaaa
     1621 gtaaataatc aggataaacc agtttcccaa accggaaggc ggcgggaagg cggattgctg
     1681 tgcttgggaa tatatgttct ttttgataaa taattttatt taaaacaaat aaatatatgt
     1741 ctttaataaa taatctatcg aaaacgaaaa atgaatttat tttaacatat atttgcaatg
     1801 aaacaggttt gccccccgtt tgtttgccct tatccctttc agtacggcat tcaagattcg
     1861 ggcctgcgcc acatccatat ggcgacaagg gaacaaaaaa ccgatgaaac cgccccgacc
     1921 caccagcgtt ggggaaactg ccaaaacatt atcaggcagg atgcggtcat catactgatg
     1981 gcgaatattt tggctttgcg cggcactgcg ccgttttgtt cccagttatg aaccatcgga
     2041 ccgaaatagc ggtgccggtg cagccagcgg tgaaagcgcg gggatgcctt tgcccagcag
     2101 gcggcggaga gcagtacgaa cggcgtggtc ggcaacagcg gcaaaaaaat gccgatgata
     2161 cccaacagca gggaaatgca gccgcaggca attaaaagat aacgtatcat tttgaaatat
     2221 ttttcttatt gtgcggataa gggcaggatg tgataccgag ttttgcccag ccttcatgtc
     2281 ccattttttc cagcagggcg atattgcgtt cgaatattgc cgaagcgtcg ggaaaggctt
     2341 gtgcggcttt ggcaatgctg tcttcgcgga tgaggtgcag cgtcggatag ggagaacggt
     2401 tggtgtagtt gccgatgtcg tcgggttcgg tattctgaaa gcggaattgg ggatggaacg
     2461 gtgcgatttg gacgatgcct tccaagccgt tttcgataac ggcggcatcg gcaatgtcga
     2521 gcatatcgtt gaatacgtcg aaatcgggga atagggtcgg gtgaaccagc agggtggttt
     2581 ccagttcggt ggcgggtgta ttgcccagtc gctgcagttc ttcgtccaag tcttccaaaa
     2641 aactgtcaag gtgtttggct tcgctgattg cgatgcggac aaggttttta acgtgggggg
     2701 ctttggcaaa gggacacagg ttcagaccga tgacggcttt ttccaaccat tgtccggtgt
     2761 gttcggcaac agcatcttta ttttcggaag tattgatatt cattattgtc atgtaaatgt
     2821 gtttgcagat tgcacgtgcg ggaaaatcgg gaagggcact attccttcag caggtggttg
     2881 agcggcaggg aggtggtgtg tttgatttct tttaaaacaa agctcgattg cgcatcttgt
     2941 acgccgtggt gggacaggag cgtatccaaa acaaaatggg aaaacgcgtt catatcggta
     3001 aaaaacgcct gaagcaggta gtcggtttcc cctgtcaggg cgaagcagct caagacttcc
     3061 ggccattttc ggaccgatgc ggcaaagtct tcccgcgcgt cttttgcttt gcggatggaa
     3121 acgcggataa atgcctgaag ccccaggttg acggattccg gagacagcag cgcggcatat
     3181 tggcggacga tgccggcatc ttccaactgc ttcagacggc gcaggcacgg agaaggcgaa
     3241 agtgcgacac gttcggacag ttcgacattg gtcagcctgc cgttttcctg gagaacctgt
     3301 aagattttaa tatcggtttt gtctaaagtg agttggggca tatttgcgtt ccgttttaag
     3361 gaattcggat tgtctgtccg tatgtttgcg gcaatccgca cagatggaga ccatattaac
     3421 atataaaaag ttataccgtc atccgggaca aattttgttt tcggaaaatc atgtgaaaac
     3481 agaggcggtc ggtttgcatc tcttttaaga cggcttgccc aaaccgccga ttcaagacat
     3541 aatcgggaaa tgtgcaggag agtgttacac ccaactacaa tgtaaccacc gaaggcgcag
     3601 acacccttaa atcgctcagg tatcagggac tgcacattga aacaaacaat ctggagagcg
     3661 gcgttggaat aacgtccacc gaaggggaga aggccgtctg aaccaccatt cagacaaccg
     3721 cgcaaagcag tgagcagact ggtttgccat catgcggata cggccgaaaa tctcaggttc
     3781 aaggacagat agggtcatcc gcgcattttg tgcggacggc atctgaacaa aaaatccgga
     3841 gaaacttgag aatgaccgct ctgaaaacca ccccgtttca ccaagcccat caggatgcag
     3901 gcgcgaagct agtcgatttt gccggctggg agttgcccat ccattatggt tcgcaaatcg
     3961 ccgaacacga agccgtgcgc accgacgccg gtatgtttga cgtatcccat atgcttgtta
     4021 ccgacgtagc aggcgcaaat gccaaagcct ttttccgcaa actgattgcc aacgatgtcg
     4081 ccaaactcgc ttttgtcggc aaagcccttt attctgcttt gctcaacgac aacggcggcg
     4141 tgattgacga cttgatcgtt taccgcacca acgaggccga aacccaatac cgcatcgtgt
     4201 ccaacggtgc gacccgcgaa aaagacacgg cgcaattcca caaagtcgga caagagttcg
     4261 gcgttgcctt caatccgcgc tacgacctcg gcatgctcgc cgtacaaggc ccgaaagcca
     4321 tcgaaaaact cctgaccgtc aaacctgaat gggcggatgt gattcacggc ctcaaaccgt
     4381 tccaaggtgc agacttgggc aacgactggt ttgtcgcccg caccggctat accggcgaag
     4441 acggcgtcga agtgattctg ccgggtactg aagcaggcgt attcttcaaa gctctgcaag
     4501 cagcaggcgt acagccttgc ggcctcggcg cacgcgacac cctgcgcatg gaagccggta
     4561 tgaacctcta cggcaacgat atggacgaca ataccagtcc gctcgaagca ggcatgggtt
     4621 ggaccgttga cttgaaagac gaaagccgcg acttcgtcgg taaagccgca ttggtggcat
     4681 tgaaagaaaa aggcgttgcc gtcaaacagg tcggcctgtt gctcgaaaaa ggcggcatcc
     4741 tgcgcgcgca tatggaagtg ttgaccgata aaggtcaagg cgaaaccacc agcggcgtat
     4801 tctcgccaag cctgaaacaa tccatcgcca tcgcccgtgt gccgaaagat tttgacggcg
     4861 ataccgccaa agtgctgatg cgcggcaagg aagtggacgt acgcgtactg aagctgccgt
     4921 ttgtgcgcaa cggacagaaa cagtttgatt gatgcggttt cagacggcat tttcatttca
     4981 tatgccgtct gaaagcaggt tttaattgtt gtccgatacg gacgtttgta gaaagcattg
     5041 aacaaggcat ctgtggatat tgattcatgc agatgccgtc tgaaaataac ccctatcaat
     5101 ggagtatcaa accatgagca acaacatccc ggccgaactg aaatacgttg ccagccatga
     5161 atggctgcgc cttgaagaag acggtacgat taccgtcggc attacccacc acgcgcaaga
     5221 gctgttgggc gacatcgtgt tcgtcgagct gcccgaagtc ggcgcgaacc ttgccgctga
     5281 agagcaagcc ggtgtggttg agtctgtaaa agccgcgtcc gacgtgtacg caccgattgc
     5341 aggcgaagtc gttgccgtca acgaagattt gccaagcgct ccggaaactg ccaacagcga
     5401 tccttacggt gcaggctggt tcttcaaact caaaccggca aacgttgccg attacgacag
     5461 tctgctgact gccgaacaat acgcgggcga agtggattaa accgcccggc tgcccgacgg
     5521 caaccgccgg acaaacggaa actgcacctt cagacggcat ttttgcggtc ggaggtgcag
     5581 ttttttgtcc gtgttttaag gaagcagtta ggctataata acggcctata ttcatcttta
     5641 ccgatttttt catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca
     5701 tacgggaaaa gctggcgttt gccgcggcct gcctgcccga agccgtccgc aatcttgccc
     5761 gaagcaaggc ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact
     5821 gtgtaggcga ttcggaagaa atcatccgtt ggctcgcaga ctatcacagc cttcccatag
     5881 aagaaatcag cccctacctt tatactttgg ggatgcagga gactgtgcgc catgccttcc
     5941 gcgttgcctg cggcttggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta
     6001 aggatgcggt cagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt
     6061 tccaaaaaac cttttctgtt gctaaagagg tccgtaccga tactgccgtc ggcgaaaact
     6121 cggtttccat ggcttccgct tccgtcaaat tggcggaaca gatttttccc gacatcggcg
     6181 atttgaatgt cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg
     6241 ccgccaaaag tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt
     6301 tgtgcgacaa gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgctattc
     6361 tgcacgatta cgacgtggtg gtttcttcaa cggcgagcca gcttccgata gtcggcaaag
     6421 gcatggtcga acgtgcattg aaacagcgtc agagtatgcc gttgtttatg cttgatttgg
     6481 ccgtgccgcg cgatattgaa gcggaagtcg gcgatttgaa cgatgcctat ctttatacgg
     6541 tggacgatat ggtcaacatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg
     6601 ccgccgaaac gctggtatcc gagaaggttg ccgaatttgt caggcagcag cagggcaggc
     6661 agagcgtccc cttgattaaa gccttgcggg acgagggcga gaaagcgcgc aagcaggtgt
     6721 tggaaaatgc gatgaaacag cttgccaaag gcgcaacggc agaagaggtt ttggaacggc
     6781 tgtccgtcca actgaccaac aagctgctgc attcgccgac ccaaactttg aataaggcgg
     6841 gggaagaaga taaagatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac
     6901 ggtgcgccgg gaaaaatgcc gtctgaagag gtttcagacg gcattttttt gtgccgcctg
     6961 acaacatcgt gaaatcccac attatatcga tgtaatcaca aa
//
LOCUS       DAWGJV010000111         6716 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWGJV010000111 DAWGJV010000000
VERSION     DAWGJV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.111"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            18..1409
                     /locus_tag="WHQ94_001581"
     CDS             18..1409
                     /locus_tag="WHQ94_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2128838.1"
                     /translation="MQVFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            1488..2744
                     /locus_tag="WHQ94_001582"
     CDS             1488..2744
                     /locus_tag="WHQ94_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ2128839.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSSKDTEIRSDDLSLSRYQA"
     gene            2925..3734
                     /gene="zupT"
                     /locus_tag="WHQ94_001583"
     CDS             2925..3734
                     /gene="zupT"
                     /locus_tag="WHQ94_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ2128840.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            3831..6668
                     /locus_tag="WHQ94_001584"
     CDS             3831..6668
                     /locus_tag="WHQ94_001584"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ2128841.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFAMPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVATL
                     PESEDAPVAVCNGARLMLKVEIDKATETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
BASE COUNT         1594 a         1821 c         1795 g         1506 t
ORIGIN      
        1 agacaaagga gaaagccatg caagtgtttt tagacaatct caaagccttt ttcgaaaccg
       61 tcagcggctg ggtctgggga tctgttatgt tgatgctgct ggtcggcacc ggcattgtgc
      121 tgaccgttat gctgaaaggc ttgcagttca cgatgttggg ctatgcgctg aaacaggcgt
      181 ttgtgccgtc aaagaagtat gaaggcggcg aaggccacga aggcgatatt tcccatttcg
      241 cggcgttgat gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga
      301 ctgcggtggt aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg
      361 gcatggccac caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca
      421 aaggcgaaat gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga
      481 aatggatggc cgtcgcgttt gcgctgttcg gcacattcgc ttccttcggt atcggcagct
      541 cggtgcagtc caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct
      601 ataccggcat tacgttgacc gttctgactg ccgttgtcgt tttaggtggt attaaaggca
      661 tcgccaaagc cgcttctttc atcgtgcctg ctatggcggt gttttatgtg ttgggcggtc
      721 tttccattat cgcgattaat tccgatgcac tgatgcctgc cgtcaagctg attttctccg
      781 atgcgttcag cgcgcaggca gtggcgggcg gcgctatcgg tacggtcatc cgctacggcg
      841 tggcgcgcgg cgtgttctcc aatgaggcgg gcatgggttc tgcaccgatt gccgccgcag
      901 ccgcgaaaac cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg
      961 ataccatcgt cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg
     1021 gcggcgagtt tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa
     1081 aaatgctgcc cggcatcggc ggctggattg ttaccatcgg cctgattttc tttgcctact
     1141 caaccatttt gggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga
     1201 aatttgccgg tttgtaccgc gtcggttatg tttcttccgt tatgctgggt accgtgttga
     1261 gccttgattt ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc
     1321 tgattgcact tttattgatg gcgaaagtca tcgtcaatga aacgcgcgac ttcaaacaaa
     1381 aaatcacaaa cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag
     1441 acggcacggc atattaagtc tgttttgaaa aagccggagg gagaaacatg aaagtgcttg
     1501 ttttaggtgc gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gcaggacatg
     1561 aagtaacggt catcgaccgc gccgagggtg tggcgatgga aaccagtttt gccaacgcag
     1621 gccagctttc ttacggctat accacgcctt gggctgcacc cggtatcccg accaaagcgc
     1681 tgaaatggct gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc
     1741 aaatcgaatg gctgtggcaa atgctgcaaa actgcacggc agcgcactat caaaccaata
     1801 aagagcgcat ggtcaggatt tccgaatata gccgtgaaat gttccgccgt tttgaagcgc
     1861 aaaccggcat gaattttgag gaacgcaaaa aagggacgtt gcagattttc cgccaaacca
     1921 aagaagtcga agcggcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc
     1981 gccgtctgaa acccgaagaa tgtgcagaat ttgagcctgc gctggcacgc gttaccgcca
     2041 aaattgccgg cggcctgcac ctgcctgcag atgcgaccgg cgactgccac ctcttcactg
     2101 aaaacctata caaattgtgt caggaaaagg gcgtacagtt tcatttcaac caaaccatca
     2161 gccgcatcga ccataacggg ctgcgcatca aagccgttga aaccgaaaca gggcggtttg
     2221 aagcagatgc cgttgtctgt gcgctcggct gcttcagtag gactgtgttg gcgcagttgg
     2281 atctcaatct gcccatttat cccgtcaaag gctattcctt gaccctgccg gtcaccaatt
     2341 cagacggcgc accggtgtcc accgttttag atgaaagcta caaagtcgcc atcacgcgct
     2401 ttgacaacag aatccgcgtc ggcggtatgg cggaattgtc gggctacgaa atcaaactgc
     2461 ccgaaaaacg ccgcgaaacc ttggctttgg tcgtcaacga cttgttcccg gaaggcggcg
     2521 atttgagcca agcatcgttc tggagcggcc tcagaccgat gacgcccgac agcacgccgt
     2581 taatcggtcg cacccgcttt gacaacctgt ttctgaatac cggccacggt actttaggct
     2641 ggaccatgtc gctgggttcg gcaaaattaa ccgccgatat cgtgagcagc aaagacaccg
     2701 aaatccgcag cgacgatttg agcctgtcgc gttatcaggc ttaagctgga aagcgataag
     2761 gtttagcggc atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggccttttgt
     2821 ttcaaaatcc gtgtatccaa tcaataaaat aaataataac tattatcata tacattaaaa
     2881 agtgttagtc tgaacctgtc ccagctttta gcgcaggttt tctcatgccc gatttttcga
     2941 tgtccaattt ggccgttgcc ttttccatca cattggctgc cggtttgttt accgtattag
     3001 gcagtggctt ggtgatgttt tccaaaacgc ccaatccgcg tgtgttgtcg tttggtttgg
     3061 cgtttgccgg cggtgcgatg gtatatgttt ccctgacgga gattttcagt aagtccagcg
     3121 aggcgttcgc tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt
     3181 tggccgggat gggcggcatt gcgctgattg accgtctggt gccgaacccg catgaaactt
     3241 tagacgcgca agacccgtcg tttcaagaaa gcaaacgccg ccatatcgcg cgagtcggca
     3301 tgatggcggc gtttgcgatt actgcgcaca atttccccga aggcttggcg acgttttttg
     3361 ccacattgga aaatccagca gtcgggatgc ctttggcctt ggcgattgcc atccataata
     3421 ttccggaggg catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa
     3481 cggtgtgggc gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt
     3541 tggttttgca gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg
     3601 gtgtgatggt gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg
     3661 gccatgaaac cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat
     3721 tgttccattt ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt
     3781 gcgttataat ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca
     3841 aatactcccc cgccgaaatc gaatccaagc actatcaaaa ctgggaaagc caaggctatt
     3901 tccaacctga tatggatttg accaaaccgt ctttttccat ccaactgccg ccgcccaatg
     3961 taaccggcac gctgcacatg ggtcatgcct tcaaccaaac cattatggac ggcctgaccc
     4021 gctactaccg tatgaaaggc tgcaacaccg cctggattcc cggcaccgac cacgcgggca
     4081 tcgccacgca aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcataact
     4141 tggatcgtga aaaattcttg gaaaaagtgt gggaatggaa agaagtttcc ggcggcacga
     4201 ttacgcagca gatgcgccgt gtgggctgtt ctgccgactg gacgcgcgag tatttcacaa
     4261 tggacgatgt acgcgccgaa accgtaaccg aagtgttcgt acgcctgtat gaacagggct
     4321 tgatttatcg cggcaaacgc ttggtgaact gggatccggt attgggtacc gcagtgtctg
     4381 atttggaagt ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg
     4441 cggacaatcc tgccgaagcc gtcatcgtcg ccaccacccg tcccgaaacg ctgttgggcg
     4501 acgttgccgt tgccgtcaat cccgaagacg aacgttatac ccacttaatc ggcaaagaat
     4561 taatcctgcc gctgaccggc cgcaccattc ccgtgattgc cgacgaatac gttgaaaaag
     4621 acttcggcac cggctgcgtg aaaatcacgc ctgcgcacga cttcaacgac tacgaagtcg
     4681 gcaaacgcca cgatacgcgc ttggttaatg tgttcgactt ggaagccaaa gtattggcaa
     4741 acgccgaagt gttcaacttc aaaggcgaag cgcaaccaag ctttgccatg cctgaaaaat
     4801 acgcaggctt ggaccgcttt gccgcgcgca aacaaatggt tgccgatttg caggaacaag
     4861 gtttcttggt cgaaatcaaa gcgcacacgc tgatgacgcc gaaaggcgac cgaacaggct
     4921 cagtgattga gccgatgctg accagccaat ggtttgtcgc catgtccgct acgccaaacg
     4981 gcggcgagcc tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg
     5041 tcgacagcgg cgcggtacgc tttatccccg aaaactgggt caacacctac aaccaatgga
     5101 tgaacaacat ccaagactgg tgcatctcgc gccaactgtg gtgggggcat caaatccccg
     5161 cgtggtacga cgaagcaggc aatgtttacg tcgcccgcaa tcaggtggaa gccgaaaaac
     5221 aagccggcaa aacaggcttg acccgcgaag aagacgtatt ggacacttgg ttctcctccg
     5281 cgctcgtgcc gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc
     5341 tgccgtccaa cgtcttggtt accggctacg aaatcatctt cttctgggtg gcgcgtatga
     5401 ttatgatgac cacccacttc accggcaaag taccgtttaa agacgtgtat atccacggca
     5461 tcgtgcgcga ccacgaaggc aaaaaaatgt ccaaatccga aggcaacgtc attgaccctg
     5521 tagatttgat cgacggcatc ggcttggaaa aactgctggt aaaacgcacc accggcctgc
     5581 gcaaacccga aaccgcgccg aaagtggaag aagccacgaa aaaactcttc cccgaaggca
     5641 ttccgagtat gggcgcggac gctttgcgct tcactatggc gagctacgcc agcttggggc
     5701 gttcggtcaa cttcgacttc aaacgcgccg aaggctaccg caacttctgc aacaaaatct
     5761 ggaacgccac caacttcgtc ttgatgaaca ccgaaaacca agactgcggt tacggcgcaa
     5821 ccgctgccga accgcgcggc tactccttcc ccgatatgtg gattgtaggc cgtctgaatc
     5881 agaccatcga gcaagtaacg caagcctacg aaacctaccg ctttgatttg gcggcggaaa
     5941 ccctgtacag cttcgtgtgg aacgactatt gcgactggta tctggaactt gccaaagtgc
     6001 agctccaaac cggctgcgcc agccgccaac gcgccacacg ccatacctta ttgcgcgtac
     6061 tcgaagccgc cctgcgcctg ctgcacccga ttatcccgtt catcaccgaa gaactgtggc
     6121 aaaccgtcgc ccctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc
     6181 ccgaagccga cggcggcgat attgttcaga cggcattcgg gcaaatgacc gtgttacagg
     6241 atttgattgg cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aacgtgaaag
     6301 ccccgctgtt tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga
     6361 tgacccgact gaccgaagca cagcaagtcg ccaccctgcc cgaaagcgaa gacgcgcccg
     6421 tcgccgtctg caacggcgcg cgcctgatgc tgaaagtcga aatcgacaaa gccaccgaaa
     6481 ccgcccgttt gagcaaagaa gccgaaaagc tgcaaaaagc cttggacaaa ctcaacgcca
     6541 aactctccaa acccggctac accgaaaaag ccccggcgca tttggtggaa aaagacaaag
     6601 ccgatttggc ggaattggaa gacaaaatgg caaaagtgca aactcagttg tcgaagttga
     6661 aagactgatt tgattgaatc aacaaggccg tctgaaaaat gtttttcaga cggcct
//
LOCUS       DAWGJV010000112         6715 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWGJV010000112 DAWGJV010000000
VERSION     DAWGJV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.112"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WHQ94_001585"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WHQ94_001585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ2128842.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1775..3130)
                     /locus_tag="WHQ94_001586"
     CDS             complement(1775..3130)
                     /locus_tag="WHQ94_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ2128843.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVTTAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(3145..3417)
                     /locus_tag="WHQ94_001587"
     CDS             complement(3145..3417)
                     /locus_tag="WHQ94_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ2128844.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3543..4280)
                     /locus_tag="WHQ94_001588"
     CDS             complement(3543..4280)
                     /locus_tag="WHQ94_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ2128845.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4561..5460)
                     /gene="prmB"
                     /locus_tag="WHQ94_001589"
     CDS             complement(4561..5460)
                     /gene="prmB"
                     /locus_tag="WHQ94_001589"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ2128846.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5605..6198
                     /locus_tag="WHQ94_001590"
     CDS             5605..6198
                     /locus_tag="WHQ94_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128847.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            6310..>6715
                     /locus_tag="WHQ94_001591"
     CDS             6310..>6715
                     /locus_tag="WHQ94_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ2128848.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANP"
BASE COUNT         1839 a         1666 c         1533 g         1677 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgcttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgctct ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat aacggaaata cagatggaag cattcgattt gggtactgaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc gggcttggcg ttgtacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tccacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgggtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gtgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcaca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taaccgggtt caaacgaaaa tgccgtctga
     1261 agcctttcag acggcataaa tgcgttttga caaaatcagt aaaagacatt gccaaataga
     1321 aaatactgat aatgcccaca atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa
     1381 atcagaaacc taaaatttcg tcattcccgc gcaggcggga atccggtttt ttgagtttcc
     1441 gtcatttctg ataaattctc gtcgtttttc atttctagat tcccgcctgc gcgggaatga
     1501 caaaattaaa gtttcagaat ttatttgaac aacaaactag attcccactt tcgcggggat
     1561 gacggcggat aggttttgtt gtttcggata aattcctgtt gttttttggt ttccagattc
     1621 ccacctttaa aagaatgacg taagaaagca gaaataagga cacagggatt ttttaaattt
     1681 gcaatattct tttctaaaga cattttaccc gatgcctata aacaaaaatg ccgtctgaag
     1741 cctttcagac ggcatttccc catcaaaacc gcaatcagtt tttcatcgat tgaaccggtg
     1801 cgggaatcct gccgccccgg ttgacgaaca cttcgcatga gccttctttt accggcatta
     1861 caggcgcgta gcccaacagg ccgccgaact cgacgctgtc gccgacggtt ttaccggtta
     1921 ccggaataat gcgcacggca gtggttttgc tgttgatcat gccgatggcg gcttcgtcgg
     1981 caatgatgcc ggaaatggtg tgcgcgggtg tgtcgccggg aacggcaatc atatccaagc
     2041 cgaccgaaca aacggcggtc atggcttcga gtttgtccag cgtcagcacg cctgcttcgg
     2101 cggcggcaat cataccttcg tcttcggaaa cggggataaa cgcgccactc aaacccccga
     2161 ccgcgctgga agccatcatg ccgccttttt tcacggcatc gttcagcaat gccaaagctg
     2221 ctgttgtgcc gtgcgtaccg cagacgctca agcccatttc ttcaagaatg cgtgccactg
     2281 agtcgccgac ggcgggggtc ggcgccagcg acaagtcgag aataccaaac gggatattca
     2341 gcatttttga ggcttcgcgg ccgatgagtt cgcccacgcg ggtaattttg aaagcagttt
     2401 tcttcactac ttccgcaact tcggtcaatg tcgttgcatc tgaattttcc aacgcggctt
     2461 ttacgacacc tgggccggat acgccgacat tgataacggc atccgcttcg cccgaaccat
     2521 gaaacgcgcc cgccataaac gggttgtctt ccaccgcgtt gcagaacacg acaattttag
     2581 cgcagccgaa accttcgggc gtgatttccg ccgtgcgttt gacggtttcg cccgccagct
     2641 tgaccgcatc catattgata ccggcacgcg tactgccgat attgatggag ctgcacacaa
     2701 tatcggtagt cttcatcgct tcgggaatgg agcggattaa cacctcatcc gaaggcgaca
     2761 tccctttttg caccaacgcg gaaaaaccgc cgataaaaga cacaccgatg gctttggcag
     2821 ctttatccaa agtttgcgcc acgctgacgt aagaatcagc atgggtggcc gccgcgattt
     2881 gggcaatcgg cgtaacggaa atgcgctgat tcacaatcgg tacgccgtat ttggcagaca
     2941 gatattttgc cgtagtgacc aagtctttgc cgactgtggt aattttattg taaatatttt
     3001 ggttcaacac attgatatcg ctgctgatgc agtcgtgcaa atcaatgccg atggtaatgg
     3061 tgcggacatc aaaattctgg tcggcaacca ttttgacggt ttctaaaatt tcgccggatt
     3121 ggatactcat cacattcctc caactcaaat gcggtgcatc gcttggaaga tttcttcgtt
     3181 ttgcatacgg atatcaagcg cgagtttttt gctctcttcc gcaaacaaat ccaaaacctc
     3241 ttgacgcgat ttgctgcatt ttgaagtgtc caccaagata atcatagtaa aaaaatcgtc
     3301 catcagctgt tggctgatgt tgagaatatt gatttggttt tccgccaaaa ttttggaaac
     3361 atcgtacacg atgccgacgc ggtctttacc gatgacggtg atgactgaat tgttcacagg
     3421 cttactcctt gcagatatcc gttaaagtcc gaaattatac caccgttgga ttttgaagaa
     3481 atattgtcaa caatatatac atacaaaatg ccgtctgaaa ctatttcaga cagcatcaag
     3541 attcagggtt cgattaaata accatcctta tcccactggg ttttcctgac caacttgtca
     3601 tcctgataaa cagcttcgct ctttttagaa ccatcttcat accactccaa aaccaccccg
     3661 ttgcgttgat ggtggcggat agacagttcc gagagtaatc ggccgctttc atcccaagtc
     3721 agaattttgg caggctcatc gttgaccata accatttccg tcttgatact gccgtcggca
     3781 taccattgct tccatacgcc gtttgcctta ttttgcttaa actggatttc gctttccttg
     3841 ccgccgttac ggtaatagcg gtatcccgta ccctcactca agccattttt ataaggcata
     3901 acggcagatt ttttaccgtt cggataccag ttgacccact ccccgtccgg cttacccttg
     3961 ctgaagcccc ccgccatttt tttctgacca ttaaaatgcc acaaaatcaa cataccgttt
     4021 tgcagggtag gcacaaaaga tttgatttgc gttgaagcaa cgatataagg ttcagaatat
     4081 ttcttcatcg acggataata aaaatcctgc gcgtgcgcaa tacccgccac cacactatat
     4141 tgcctgatat aagcggcaga agacatcgtc gccgtcagct ttccgttctg attaaaataa
     4201 acagaatagg tctgcgccgg caaagcggcc gaaaaaccca acaggacagt tgaaaataca
     4261 atccgagata attttttcat tgcaatagcg atataaaaac aaggctgtgt tttagtaatc
     4321 tgttgatttc aattatttgc aagggaaaag acaattattt tccggttagg aataaaccta
     4381 ttctattgaa tatattgaag ccaagtacgc ctatcaacac tatattaaaa cactgccaaa
     4441 aacaattaac ttataaacaa tatggtaagg atttctctgc caagcatcaa acccgagaca
     4501 acgtatcgta aaaatgccgt ctgaaaacaa atcgtcttca gacggcattt ccccttcaac
     4561 tcactcttca cccaataact gctcgcgcgt caagaggaaa acaaaaccgt cgcccccgct
     4621 ggtttccaac caagtaaaag gcaactccgg atacgctgct tccaatacat ccctgttatg
     4681 cccgatttcc accagcaata cacctttggg attcagaaac tttgccgcat tcagaagaat
     4741 ctgcctggtg gcatccaacc cgtccgcccc gctgcccaat gccaattccg gttcgtgcaa
     4801 atactcttca ggcaataact caaccgattc cgcatccaca taaggaggat tggaaacaat
     4861 caaatcataa gtgccttcca atccttcaaa caaatccgta tgaataagcc ggatgcgttc
     4921 ttccaaacca taatcttcga cattaatccc tgccacttcc aaagcatcca agctcacatc
     4981 aaccgcatca atttgggcat caggataatg atgcgccatc tgaatggcaa ggcaaccgct
     5041 tccggtgcaa agatccaaag cattatgcac caactcatcg tattctatcc aaggacgaag
     5101 tccgtcaccc aacaattcat aaataaaaga acgaggtatg attacgcgct catccacata
     5161 gaaatcaaac tctccctgcc atgcctggtg tgtcaaataa gcggctggaa tgtgttcgac
     5221 agcacgacgc tcaataaccg ccagcacttc ctctttttca gcttccaaga gttttgcatc
     5281 aagatatggg gcaagcatat ccaaaggcaa attcaaagta tgcagaatca aataagctgc
     5341 ttcatcatgc gcattatctg ttccatgacc aaaaaagagc cctgcctcat taaaacggct
     5401 gactgcaaaa cgtaaaatat cgcggatagt cgtcaattct tgtgctgcct gattaaacat
     5461 aatatgaacc attctgcgta tagatacttt taattataac agaaacaaca agcaaacctt
     5521 ttcatatcgc caaataacca cccaatctac ccatacaact acataaatgc ccgcgcgaaa
     5581 accatcgccc gaacggaaac gacaatggcc gacggtatgg gcaatctgat tggctgggaa
     5641 aaaacggggc ttgttgtcgg taagcagtgg ataaccgcaa aagacgacaa ggtgtccgat
     5701 gtctgcaatg ccaacggcga gatgggcgta atcgggcttt acgagccttt ctcacacggc
     5761 gcattgacga tacccggtca tccgaactgc cgatgcgagg ttgtttccgt atcgggtggc
     5821 gaattggggg aatttgccga aaaaaaggag cttcgtaaag cggctatgca gtatgcgcgg
     5881 gataacttta tcggcaaaag ctatgtcaat aaaaacagcg ggcatgaact gaaggtaact
     5941 tggcaaggtg tgaaacacgc tgcgtcaaag gcaaatcagg cggaattatc catcatgaca
     6001 aaacttgatg acttattgcg ctacgcaaaa tatgagggtt cttattcgga taggaaaggt
     6061 catcctaata ttattgcagc acataagtat cgtgccgttg ccaaggttgg gaatgagtct
     6121 ttaaatatcg gtgtgattgt aagggaattt ccagacgacc ataaacatta cgaccatttc
     6181 atcttgaagg atgaataaag cccttttgca gtgtcgttct ggagcggata gcgttaaggc
     6241 aagtacactt ccagccttga aaaagggctt taaattcagc atgccattta tacaggcagg
     6301 agtaaaccca tgacaaagtt atacgcagaa atcgccaaga tggagacgca ggacgacgac
     6361 acggtcaagg tttggggtta cgcttcaagc gaggaaatcg attcggacgg cgaagtcatc
     6421 gcggcggcag ctatgaaggc ggcgattccc gattatatga agtttggcgc ggggcgcgag
     6481 atgcacggct caaacgctgc gggaacggca attgaaatca acgttgaaga cgacggcata
     6541 acctttttcg gtgctcatat catcgacccc gttgtcgtgt cgaaagtcaa aacaggcgtt
     6601 tacaaaggct tttccatcgg cggcagcgtt accgcccgcg atgatttgaa caagtcgcaa
     6661 atcacgggct tgaagctgac ggaaatcagc cttgtcgagc gccctgccaa tcccg
//
LOCUS       DAWGJV010000113         6697 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWGJV010000113 DAWGJV010000000
VERSION     DAWGJV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.113"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            37..285
                     /locus_tag="WHQ94_001592"
     CDS             37..285
                     /locus_tag="WHQ94_001592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128849.1"
                     /translation="MPSERSFRIGDRDIRNAGRLPVTAGQAVCFAGIGEGKSEMRVGV
                     YFDAAAFRRYGKRRKSSKSASDGISGKPPETCRIWVTR"
     gene            complement(275..1366)
                     /gene="ychF"
                     /locus_tag="WHQ94_001593"
     CDS             complement(275..1366)
                     /gene="ychF"
                     /locus_tag="WHQ94_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ2128850.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVELCKKLLPHLDEGKPVRSFGLDAEERAMLKQLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1479..3155)
                     /locus_tag="WHQ94_001594"
     CDS             complement(1479..3155)
                     /locus_tag="WHQ94_001594"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ2128851.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGGPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPVCMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            3330..3761
                     /locus_tag="WHQ94_001595"
     CDS             3330..3761
                     /locus_tag="WHQ94_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ2128852.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPSTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4007..4702)
                     /locus_tag="WHQ94_001596"
     CDS             complement(4007..4702)
                     /locus_tag="WHQ94_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ2128853.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHHPDGDFLLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4718..5218)
                     /gene="hpaC"
                     /locus_tag="WHQ94_001597"
     CDS             complement(4718..5218)
                     /gene="hpaC"
                     /locus_tag="WHQ94_001597"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ2128854.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(5333..5773)
                     /gene="nadR"
                     /locus_tag="WHQ94_001598"
     CDS             complement(5333..5773)
                     /gene="nadR"
                     /locus_tag="WHQ94_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ2128855.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6116..6625)
                     /locus_tag="WHQ94_001599"
     CDS             complement(6116..6625)
                     /locus_tag="WHQ94_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ2128856.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
BASE COUNT         1536 a         1880 c         1727 g         1554 t
ORIGIN      
        1 aagttaatcc actatactcg aaacgcggcg gcgcaaatgc cgtctgaaag gtcatttcgt
       61 atcggggatc gggatattcg gaatgccgga cggcttcccg taacggcggg gcaggcggtt
      121 tgttttgcag gaatcgggga gggcaaatcg gaaatgcggg tgggagttta ttttgatgcg
      181 gctgcattcc ggcggtacgg gaaacgccga aaatcatcaa aatcggcttc agacggcatt
      241 tccggcaagc cgcctgaaac ctgccgcatt tgggttacac gttaaacaaa aagtgcatca
      301 catcgccgtc ttgcacgaca tattccttgc cttccacacg cattttgccg gcttctttgg
      361 ctttggcttc gccgccgagc gagacaaagt cgtcgtaaga aatgacttgg gcgcggatga
      421 agccgcgttc aaaatccgta tgaatcacgc cggcggcttg cggcgcggtg tcgcctttgt
      481 gtatcgtcca cgcgcggact tctttcacgc cggcggtgaa gtaggtttgc agccccaaaa
      541 ggtcgtagcc ggcacggatg aggcggttca ggcccggttc ttccaagccc atttcggcga
      601 ggaactcggc tttttcgtcg tcttccaatt cggcgatttc gctctccatc gcggcgcaaa
      661 cggcgacgac gggggcgttt tcttttgccg ccaattcttt caggcgttcg aggtgcggat
      721 tgttttcaaa accgtcttcg gcgacgttgc ccacatacat cgccggtttg gcggtcagca
      781 ggaacagctg tttgagcatc gcgcgttctt ccgcgtccaa accgaaggaa cgcacgggtt
      841 tgccttcgtc cagatgcggc agcagttttt tacacagttc gaccagtttt tgcgcgtctt
      901 tgtcgccgga acgggcgcgt ttttcttcgc ggacgatggc tttttccaca cttgccaagt
      961 cggcaagtgc caactcggta ccgatggttt cgatgtcggc aatcggatcg acgcggcctg
     1021 caacgtggac gatgttgtcg tcgtcaaagc agcgcacgac attcacaatc gcatcggttt
     1081 cgcggatgtt ggcaaggaac tggttgccca agccctcgcc tttgctcgcg cctgcaacca
     1141 aaccggcaat atcgacaaat tcgacgatgg caggctgcat tttttgcgga ttgacgattt
     1201 ttgccaattc ggccatacgc ggatcgggga cttcgacgat gccgacgttg ggttcgatgg
     1261 tacagaaagg atagtttgcc gcttcgatac ccgattgggt cagcgcgtta aaaagggtgg
     1321 atttgccgac gttgggcaaa ccgacgatgc cgcatttcaa actcatgttt tttcctgaaa
     1381 atagagaaat ttaacggcgg attatagcat accgccgccc gcgttccgaa aaatgccgtc
     1441 tgaaacggct tcagacagca tccggtttca aaaaccgttc agaacaagcc gtgaatcacg
     1501 ccttccgcgt ccacgtcgat tttctcggca gctggaactt tcggcagacc cggcatcttc
     1561 atcatgttgc cgcacagggc gacgatgaaa cctgcgcctg cggaaacagt gataccgcgc
     1621 acggtgatgc ggaagccttc tgggcagccc aaaagtttgg cgttgtcgct caaagaatat
     1681 tgggttttcg ccatacagac cggcattttg tccaagccca gtttttccag tgaggcgatt
     1741 tcggcagacg cttccgcgct gaaatcgaca tcttcagcgc cgtatacttt ttgggcaatc
     1801 gcacggattt tgtctttaat actcagctca acatcgtagg aaaaattgaa gttgttcggt
     1861 tggttttcga tggcgttgac gactttgcgc gccaagtctg caccgcccac gccgcctttg
     1921 ccccacactt cggtcaagga aacttcaacg ccgtgttcgg cacaggcttt ttcaatcatc
     1981 gccaactcgg catcggagtc ggacacgaag cggttgagcg caacgacgac gggcagtccg
     2041 aatacgtttt tcaggttgga aatgtgtttc agcaggttgg gcaaaccttt ttccaaagcg
     2101 tctaaatttt cttcgccgag gttggcgcgt tccacgccgc cgttatattt caacgcgcgg
     2161 acagtcgcca cgacaacagc cgcatcaggt ttcaaatccg ccagacggca tttgatgtcg
     2221 cagaattttt ccgcgcccaa atccgcgccg aagcctgctt cggtaacggc gtaatcggca
     2281 aggtgtttcg ccagacgggt tgcggttacg gagttgcagc cgtgggcgat gttggcgaac
     2341 gggccgccgt gtacgaaggc gggcgtgcct tcgatggttt gtaccaagtt aggcttaatc
     2401 gcatctttga gcaatgccgc catcgcgccg tgtgctttca aatctttggc gtaaacgggg
     2461 ccgccgtctt tggcgtaggc gacaaggatg ttgcccaaac gctctttcaa atcactgatg
     2521 tctttggcaa gacagaatac cgccatcact tcggaggcaa cggtaatatc gaaaccgtcg
     2581 ggacgcatca cgccgtcaac aggtttgccc atgccgtcga tgatgttgcg caactgacgg
     2641 tcgttcatat cgaccacgcg ccgccacagc acgcgtttgg ggtcgatgtc caactcgttg
     2701 ccctgataga tatggttgtc gagcatcgcg gcaagcagat tatttgccgc accgatggcg
     2761 tgaaaatctc cggtgaagtg caggttgatg tcttccatcg gcaaaacttg ggcatagccg
     2821 ccgcctgccg caccgccttt aacaccgaac accggcccca aagaaggttc gcgtagggcg
     2881 ataaccgagt ctttgccgat atggcgcaac gcatccgcca aaccgatggt tactgtggtt
     2941 ttaccctcac ccgcaggagt cggattaatg gcagtaacca aaatcagacg gccttgtttt
     3001 tgcggcagct tgaatgcttc ggcaggattg attttggctt tgtagtgacc gtaaggctca
     3061 atgttgtcgg cgttcaggcc cagcttggcg gcaatttcgc caatcgggcg catggtggaa
     3121 gactgggcga tttcggcatc ggttttgaag ctcatgattt tcctttagaa atgaggaggg
     3181 acataccgtc tgaaagcatc aggcgacaaa caggtggatt gaaaataata tcaggcatat
     3241 tataacgtta tccgcaccaa acccgcagtg aaatttttga cgctgcaaca aaaataccgt
     3301 tcatattgtt cacaatccaa ggagaaaaca tgggcagcaa cgcatggctg ttttgggcat
     3361 tggcatcggc aggcttcgcc tcgctgaccg ccattttcgc caaaatgggt ttgcagggca
     3421 tcgattcgga tttcgccacc tttatccgca ccttggtgat cctcgccgct ttggtactgt
     3481 ttttgaccta caccggcaaa tggcagggcg tgaacggatt tacaggaaaa aactggacat
     3541 tcttaattct gtcgggtttg gcgaccggcg catcctggct cgcctacttc aaagccctgc
     3601 aactgggtaa cgcctcgcaa gtcgcccccg tcgacaaatt cagcctggtc ttggtcgcgc
     3661 tgatggcggt ggttttcttg gacgaacgcc ccagcacgca ggaatggata ggcttggggc
     3721 tggtaacggc gggcgtgttg gtgctggcgt tgaaacgtta aaccgaatcc gccataccgc
     3781 ctgaaaccgg gttttgacaa tctgacgggc ggcggtctga acaaagttac ccaataaaat
     3841 ccgacaccgc gccgacggca aaccgccgca tccgacaggg gtcggcttca gatggcattt
     3901 gttggacgga aagccccgaa cgccctattt tcacgcctcc acgaaaatat gccggacatc
     3961 cggtcggaac cgttcggcag ccggctcggc aagccaccgt tttatttcac ttccaagccc
     4021 ctgcaagggc ttgagcctct ttcagacggc ataccgtgcc gacatccagc cacaagcccg
     4081 tatgcttctg accgctcacg cggttttgcc gcatttcgcc acgcaatacg ggcgcgagtt
     4141 tcgccacact gcccgcttcg attccgtcaa acatttcagg acggtaaata cccacgccgc
     4201 tgaatgtcaa tccgttgccg ccatttactt ccggccgcac gctgccgtcc ggcaacagca
     4261 aaaaatcgcc gtcaggatgg tgcggcggat tttccaccag ccacagatgg gcggaaatat
     4321 gttccggcag ggacgatgcc gtctgaaacg cggcggtaaa atcgatgtcg gtcagcacgt
     4381 cgccattgac gaccaaaaac ggggtatcgc ccaacagcgg caatgcccgt gcgataccgc
     4441 ctgcggtttc caagccgcct tcaggttcgg gcgaataggc gatgttcacg ccataagccg
     4501 agccgtcgcc caaagcatct tctatctgcc gacccagcca agcgtggttg atgacgattt
     4561 cggtaaaccc cgcctgcttc agacggcata ggtgccaacc gattagaggc ttacccgcca
     4621 catcgagcag cggcttcgga gtggtatcgg tcaaagggcg catacgctcg ccgcgtcctg
     4681 ccgccagtat catcgctttc atatatctgt ccgaatatca gtctaaaaat ctaaactgcc
     4741 gtctgaaata cagcagcgcg gggcgtttgc acccgcagtt tttgatttcg tcgagcctga
     4801 cgtaaaacac aaaatgcgtg ccgatttcat gtttgccgac aatatgcccg tgcaggtgcg
     4861 ccaacgcgcc ttctatttca agttgtcccg ttttgccgcg atgccagatg tggtaggcaa
     4921 accgctcttc gggcgacagg ccggtcaatc cggcaaaatg ttcggcaaca tcctgatgtt
     4981 cgtccgccag cgtattgatg cagaggctgc cgttttccga caggatcgga atgattcgcg
     5041 cactccggtt gatgcacagc atcacggtcg gcggctcgtc ggtaaccggc gcaaccgccg
     5101 tcattgtaat gccgtaacgc cctgccgcac cgtctgtcgt gatgacatga acgcctgccg
     5161 cgcaagatgc catcgcatca cggaacgaag tttgaaaagt tttctgcaaa tccgccattt
     5221 ttccccttta aactgtcccc tatataagaa tgctgcacac aaggcacccc ccatgtgcag
     5281 cagttctgat tcaaaaagcc gtcggtcgga catttccgcg cgttacggcg tattacgagt
     5341 tcaacgcatc ctcgattttg gcaagttctg ccaacaggtc tttaagcagc agcattttct
     5401 cgcggcccag cacttcctcg atagcgtcgt aacgctcgtc cacttcttcg ccgatttcct
     5461 catacagctt ctcgccctcg gcagtcagct tcagaaaaac acgtcgttgg tcgttggaag
     5521 gtttcaggcg gacgaccaaa cccgcttttt caaggcgggt caggataccg gtcaggctgg
     5581 ggcgcagaat acacgcctga ttcgccaaat cttgaaagtc cagcgtgccg ttttccgcca
     5641 aaagacggat aatccgccat tgctgatcgg taatattcgc ctgattcaga ataggcctga
     5701 attgggtcat cagggcttcc cttgcctgta tcagactgat attgatagac gcatgttttg
     5761 attgggtagg cattgttcaa gtctccaagt tatcgaaaat caaactttca aaccgtcggg
     5821 aaagcctgtg ggcgtaaatt ttgatgcaac cgttatataa caaaacgaac atatagcaac
     5881 aatacgctat aaaccgcatc ggacgactgg gtataaaaga ctttaattcc gataatccta
     5941 tctaaaaata ttttaatagt tatatcttag tctattttcc ctacaatctc aacaaagtat
     6001 tacatttact gtcgaaagaa ctttcttcca gaaaaaacaa aagccgccgc atcagacgag
     6061 caaggtatat gccgtttgat tctctacata gcgtaaagtt taacaaaaac aaaagttaac
     6121 cgaaaacatc cgcctgaaaa attcgtgcgc gcaagcccca ataactgctg attcccgtcg
     6181 tatagtgaac cattttccca tttttgacca aaacgacggc aggcgttgcg acaatccgcc
     6241 aagaccttgc caaacccccg tcctcatcgt tgacagtcgg aaagcccaag ccgcgttttg
     6301 ccatatacgc cgccacttcc gccgaactgc cggaacgtac cgccacgccg acgaccggca
     6361 cgccgtccgc cgccaaatca tcgattatcg gcgactgata acggcacacg ccgcaccagc
     6421 tcccccaaaa atacaccaaa accgccttat cccggctaaa ctgtcccaaa gtcagccgct
     6481 gccccgacag cagggtcaaa ggccgccctg ccgcgccggc aggttcgtcg ggcttgcgta
     6541 cccaatccaa aaacagcgac accaataaaa acaccaatgc cgtctgaacg gcaaatttga
     6601 tgcccgaaag cagtttcttt ttcatacgct ctctcaaacg gtacgcccgc gcaaccggca
     6661 ggcaaacaaa aagccaagtc tcaaaacttg gcttccg
//
LOCUS       DAWGJV010000114         6516 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWGJV010000114 DAWGJV010000000
VERSION     DAWGJV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.114"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            64..762
                     /locus_tag="WHQ94_001600"
     CDS             64..762
                     /locus_tag="WHQ94_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ2128857.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPYKRPNNATTKAQRESVQGKP
                     CVECGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQY
                     SKEQKRKLSNGNKK"
     gene            746..1099
                     /locus_tag="WHQ94_001601"
     CDS             746..1099
                     /locus_tag="WHQ94_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128858.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSHDENFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1089..1412
                     /locus_tag="WHQ94_001602"
     CDS             1089..1412
                     /locus_tag="WHQ94_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ2128859.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQNEFGEMWTCYCSVNIIPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1471..1698
                     /locus_tag="WHQ94_001603"
     CDS             1471..1698
                     /locus_tag="WHQ94_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128860.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1825..2367
                     /locus_tag="WHQ94_001604"
     CDS             1825..2367
                     /locus_tag="WHQ94_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ2128861.1"
                     /translation="MINHIEKYTGTISHGSKNDSGNRYKLNIAAIPSSPNSDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNTVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            2502..3207
                     /locus_tag="WHQ94_001605"
                     /pseudo
     CDS             2502..3207
                     /locus_tag="WHQ94_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            3216..3488
                     /locus_tag="WHQ94_001606"
     CDS             3216..3488
                     /locus_tag="WHQ94_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128862.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            3655..3885
                     /locus_tag="WHQ94_001607"
     CDS             3655..3885
                     /locus_tag="WHQ94_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ2128863.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            4036..4773
                     /locus_tag="WHQ94_001608"
     CDS             4036..4773
                     /locus_tag="WHQ94_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ2128864.1"
                     /translation="MVKTADGYKAIAHIRVGESVLSKDEASGKTGYKPVTARYGNPYR
                     ETVYIKVSDGIGNNQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            4770..5189
                     /locus_tag="WHQ94_001609"
     CDS             4770..5189
                     /locus_tag="WHQ94_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HEZ2128865.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            5356..6087
                     /locus_tag="WHQ94_001610"
     CDS             5356..6087
                     /locus_tag="WHQ94_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ2128866.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTAQCGNPYR
                     ETVYIEVSDGIGNSQTLISNLIHPFYSDGKWIKAEDLKAGSRLLSESGRTQTVRNTVV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNECPYGKGNQRYKDAPYHGKNDNSV
                     KSRVPTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNW
                     KNLHTDQQNALKKAGLVNSKGKIKK"
     gene            6084..6413
                     /locus_tag="WHQ94_001611"
     CDS             6084..6413
                     /locus_tag="WHQ94_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128867.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
BASE COUNT         2223 a         1228 c         1330 g         1735 t
ORIGIN      
        1 gggaaggctg cggttagtgg ggatttttct aaatcctaca cctgctcctt ccacggcagc
       61 accttggtca aaacggcaga cggctacaaa gccattgccc atattcaagc cggcgaccgc
      121 gtcttcgcca aagatgaaac cagtggaaaa actggataca aacccgttac cgcccaatac
      181 ggcaatccgt atcgggaaac cgtttacatt gaaatttcag acggcatcgg caacaaccaa
      241 accctgattt ccaataaaat ccacccgttt tacagtcaag gaaaatggat acaggcaggt
      301 cgtctgaaaa aaggcgacac cctgctttcc gaaagcggtg caaaacagac ggttcaaaac
      361 attaccctca aacagcagcc gctcaaagcc tacaacctga ccgttgccga ctggcatacc
      421 tatttcgtca agggtaatca ggcggaaacg gaaggagttt gggttcataa ttcttgtccg
      481 ccatataaga gaccaaataa cgcaacgaca aaagcgcaac gagaaagtgt acaaggaaaa
      541 ccctgcgttg aatgcggtaa attagccgaa aaaatgattg ccgaccataa aaaaccatta
      601 gttgtcgagt attatgaaac aggaacaatt gacaaatcta agatgagagc aattgaatca
      661 gtacaaccgc aatgtccgac ttgttctgca aaacaaggag ggagattaag ccaatattca
      721 aaagaacaga aaaggaaact gagtaatggg aataaaaaat gaacaaataa taatttgtaa
      781 aaaatataat acagaaatat atcctgtttc cgatgtttca aaaatcggtg ttgctgaaaa
      841 tgtcaaacag gcaaacctgt atccgattaa cgggttaagg caccgtccca aaggtgatac
      901 taacggttgg tatatctggg ccggtgaaaa cttctcccat gatgaaaatt ttttcctgcc
      961 cctgcatact ttccatttgc aaatatggcg acccgaaatc atcccctttt tgacacttcc
     1021 gcccggttac cgttttttaa ttggggaaaa cggttatgaa gatgtatggt ttgatgagtt
     1081 gctattaaat gataattgac gaaaaatcta cttcagatgt actgaaacaa tatcaagaac
     1141 tagggtttga tttgacaaaa cctatgataa tagagttttt tataggaggt tctcaaaaaa
     1201 atctgcaatc aatagaaagc caactcattt cttatcggca agattttcaa atcagtattg
     1261 agcaaaatga attcggagaa atgtggactt gctattgttc cgtcaatatt atcccttctc
     1321 ttgaaaacat tttagctatt gaaactacgt tatttggtat tgcacaaaaa aatgattgta
     1381 attatgaagg ttttggttcg tatgggaact aacaaaaagt gtaggttggg ttgaaaaaac
     1441 catattccca ttaaagatta gggtgtgtct atggacataa aattcaatac tttgggggtt
     1501 atcctgaatg gggtcaatcc tgaagaaaaa ttcataaaaa ttattgatga tcaggaaaat
     1561 accgggggat ttctgatact gttatcttca aatgacaaat tctcatcatt tgacagttac
     1621 gatgactggg tcgaaaactt agaaatatta aaagaatacc tgcaagaatc tcattggata
     1681 ataaagtggg tcggatagtc ggcaaacgta gtccgtctga aacactttta cttttcagac
     1741 ggactttcta tcaatgggaa caaggcgttt tgtctcaatc tggtctatga ttattcgata
     1801 cagtgatggg aagctaagaa aaatatgatt aatcatattg aaaaatatac aggcactatt
     1861 tctcatggct cgaaaaacga ttcgggaaat cggtacaaat taaatatcgc agctattccc
     1921 tcttccccta acagcgattt aaaaacttat attactttag ggttaagcaa acatgatctg
     1981 aattataaaa gcagatttga aatacttttt gtatgttctt taaaatatga tgaaaatcag
     2041 attttcccat ttttaagatg gttggcagaa accatcattg aaaacaaaaa aatccttctt
     2101 cgggggcagg ttgtttattt gcccagaagc attgttgatt caacaaaaat ggatgcactg
     2161 tatgtttccg ccccatttta tttcgacgat gattttcagg tctgttatgg cgaacactac
     2221 aataccgttt tccctttgct tgtcccgttg tataaacagg aagcggaatt ggtggaaaaa
     2281 aagggttgga atgcttttga gcagtttttg ctggataatg aagttgacaa cctttcggat
     2341 atgaatagga aaccgtttgt ttggtaaggg ctttcttaaa tttgattaaa ggtcgtctga
     2401 aaccgtgttt aactttcaga caacctttcc ttcatttgaa acaggatatt gagaactgag
     2461 tttttcaaaa atcctacacc tgctccttcc acggcagcac tttggtcaaa acggcggacg
     2521 gctacaaagc catcgcacat atccaaactg gggagtacgt ctttgccaaa gatgaagcaa
     2581 gcggaaaaac gggatacaaa cccgttaccg cccgatacgg caatccgtat cgggaaaccg
     2641 tttacattga aatttcagac ggcatcggca acaaccaaac cctgatttcc aaccgcatcc
     2701 atccgtttta ttcgggcggc aaatggatta aagcggaaga tttggaagcg ggaagcaggc
     2761 tgttatccga aagcggcaga acccaaaccg tccgcaacat cgttgtcaaa ccaaaaccgc
     2821 tcaaagccta caatctgacc gttgccgact ggcatactta ctttgtcaaa ggcgataagg
     2881 cggaaacgga aggggtttgg gttcataatg attgtccgta tggaaattta tcggataata
     2941 aatctgttgg cgaaggaaaa aaatttacac cagctcaaaa gaaagcaatc atccaagaaa
     3001 acatgaatag aaacggaggg gtagtgaagt ctgaccaatc tggagaagtt ttggttcgtc
     3061 ctaaaaagag ccaaaaaggt atcacccccc ccatcaaatg aatggcagat tgaccatgtg
     3121 caagcaaaaa gtaaaggagg ttcaaatagt tataaaaatg cacaggtttt atccagaaga
     3181 gaaaatatta agaaatcaaa taaataggta tcaatatgca atggaaattt gaagatcctc
     3241 aaaatactgc tgttattacc accagaagaa ttttgtctaa aaaaaacgga atcttagaag
     3301 tatggcatga tgctgatgat ggaatgtggc aattcttaga tggcaatgaa attcatgaag
     3361 gagatgctgt gatagttagt ttaaaagaaa ttgtcactat tgattcctca gtcaatctct
     3421 taagtaattt acctcttggt ggttacgctt ggagaaatac tatatcggaa aaatggaatt
     3481 ttgattagca agcgtaggtt aggttaaaaa aaccaacaat caaaggtcgt ctgaaaccgt
     3541 gtttagtttt tcaaacgacc tttccttcaa ttaacataga atgctgggtc tcgacccaac
     3601 ctacacgctt actaatctca aatataacct tatgaaataa tgagtattta aaagatggaa
     3661 acattagacg aacgaataaa aaatttagga aaatctcttg aagatagaat tgacgctaat
     3721 ctaattgatg ccgcactaga gtacattact ttttcagaac gtttgctagc ttttgaaacg
     3781 ctatgtgatt atatagaaga tttcaatatc cagcttacgg aaaaagaatc ccaagaaatc
     3841 tcttttatta ataaggaatt tggaattgaa agtacatcag attaataaat attaggtcgg
     3901 tttaaaaacc caacaatcaa aaagtcgtct gaaactgtgt ttaatttaca gacgacctat
     3961 ttccttcatt tgaaacagga tattgggaat taagtttttc aaaaatccta cacctgctcc
     4021 ttccacggca gcaccttggt caaaacggca gacggctaca aagctattgc ccatatccga
     4081 gtcggcgaga gcgtcctttc caaggacgag gcaagcggga aaacaggata caaacccgtt
     4141 accgcccgat acggtaatcc gtatcgggaa accgtttaca ttaaagtttc agacggcatc
     4201 ggcaacaacc aaaccctgat ttccaaccgc atccatccgt tttattcgga tgggaaatgg
     4261 attaaagcgg aagatttgaa agcgggaagc cggctgttat ccgaaagcgg caaaacccaa
     4321 accgtccgca acatcgttgt caaaccaaaa ccgctcaaag cctacaatct gaccgttgcc
     4381 gattggcata cctactttgt caaaggcgat aaggcggaaa cggaaggggt ttgggttcat
     4441 aatgattgtc cgtatggcgg ttcaaataat ttagagaaag caaaacttcg cgcagaacgg
     4501 cttagtaaaa acgatcgggc aggaaaagac tttactaaag caggaaaaga agccgttatt
     4561 gatttgaata ggattcaaaa taatggtcaa gttaagtgcg ctaattgcgg tattgaaaca
     4621 attccagcca aacaatctat aaaaaatatt tctcctactt caaatgaaag acaagtagat
     4681 catgtcatcc ctaaatctaa gggtggtcaa ggtacaccta aaaacgggca ggtattatgc
     4741 agaggctgca acattaagaa gagtaacaaa tgaaatcgaa acttactgtc gtctattacg
     4801 atttggaaag caatattgca gaagaaatac tgtcaggaaa cataatgcct gatggaaatt
     4861 ttcttattca agagattcct cttttcgcac cgaatttggc tttaaacgac attgttgcca
     4921 tagaacgtga ggataagatg ctgtttttcg accacttgat aaaagcttca ggaaatacca
     4981 cgataaacat cgttgttttg gatcatttcc caaaggattt attggcagcc atagaagaac
     5041 acagtggtaa aatcagaaaa aatggagaga attatttatc ggttaatttt ccgcccaaaa
     5101 aatataattc tgatttaaaa ggaattttaa atagatatga ggaagcaaat atcctcagct
     5161 acagggaagc ttgtttgggc ttctcttaat cgatctataa gtaagaatgg attacgttag
     5221 attgtaaacc taacgattaa aaggtcgtct gaaaccgtgt ttaattttca gacgacctat
     5281 ttccttcatt tgaaacagga tattgggaat taagtttttc aaaaatccta cacctgctcc
     5341 ttccacggca gcaccttggt caaaacggca gacggctaca aagccatcgc ccatattcaa
     5401 gccggagacc gcgtcctttc caaggacgag gcaagcgggg aaacaggata caaacccgtt
     5461 accgcccaat gcggcaatcc gtatcgggaa accgtttaca ttgaagtttc agacggcatc
     5521 ggtaacagcc aaaccctgat ttccaacctc atccacccgt tttattcgga cggcaaatgg
     5581 attaaagcgg aagatttaaa ggcgggaagc agactgttat ccgaaagcgg cagaacccaa
     5641 accgtccgca acaccgttgt caaaccaaaa ccgctcaaag cctacaatct gaccgttgcc
     5701 gactggcata cttacttcgt caagggtaat caggcggaaa cggaaggggt ttgggttcat
     5761 aatgagtgtc cgtatggaaa aggcaaccaa cgatataaag atgctcctta tcatggtaaa
     5821 aatgataatt ctgtgaaaag tagagtgcca acaaatgggc aagctgtcct tgataactct
     5881 gttcaagtta aatcgacatc ttctcaaaga gttggagttg ataaaaccaa taatgaaatc
     5941 gttgtgttaa accaaactag aatttttaat gacggttctg ctgagtatca tgggcatgta
     6001 aggaactgga aaaatttaca tactgatcaa caaaatgctt taaaaaaagc aggattggtt
     6061 aattcaaaag gaaaaattaa aaaatgactg ataaaagtaa aacagaaaag ttgatttctt
     6121 ctgatgataa acaaagtgtt atagatggca ttcttgatat ggtatttaat tccaaagcat
     6181 atgaagtacc gtggatttct gagaaattga tggaattatc gaaaaataaa gacttggata
     6241 ttgccggatt atcgctaacc tgtttcggac atctcgccag gctacattca aatatcggtg
     6301 attacgataa agttatttct ttactacatt caaagcaaga tgatccagag cttcaaggta
     6361 gggctgaaga tgcgttagaa gatatttctt tatttttatc tgaaaatcat taggaaccgt
     6421 aggttggatt ggaaatccaa caatcaaaat gccgtctgaa accgtgttta attttcagac
     6481 ggcatttcct tcatttgaaa taggatattg agaact
//
LOCUS       DAWGJV010000115         6496 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWGJV010000115 DAWGJV010000000
VERSION     DAWGJV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.115"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            1140..1376
                     /locus_tag="WHQ94_001612"
     CDS             1140..1376
                     /locus_tag="WHQ94_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ2128868.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            2415..2629
                     /locus_tag="WHQ94_001613"
                     /pseudo
     CDS             2415..2629
                     /locus_tag="WHQ94_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2596..3861
                     /locus_tag="WHQ94_001614"
     CDS             2596..3861
                     /locus_tag="WHQ94_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ2128869.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKSKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            3864..4421
                     /locus_tag="WHQ94_001615"
     CDS             3864..4421
                     /locus_tag="WHQ94_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEZ2128870.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGVRLKTLFNSNAYISYFFGSLCIG"
     gene            4378..5949
                     /locus_tag="WHQ94_001616"
     CDS             4378..5949
                     /locus_tag="WHQ94_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ2128871.1"
                     /translation="MLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGL
                     LTILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSI
                     DHIQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLR
                     NATEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLS
                     ALFEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYI
                     QIKMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFE
                     NINLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGL
                     SGIVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDM
                     GNILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAH
                     RQETIQSADKILNLG"
     gene            complement(6031..6333)
                     /locus_tag="WHQ94_001617"
     CDS             complement(6031..6333)
                     /locus_tag="WHQ94_001617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128872.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            6400..>6496
                     /locus_tag="WHQ94_001618"
     CDS             6400..>6496
                     /locus_tag="WHQ94_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ2128873.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVG"
BASE COUNT         2161 a         1120 c         1148 g         2067 t
ORIGIN      
        1 aaggagtgcg aacgacgttt taacaacagt gagataaaag ttcttgttcc attttaaaac
       61 aattagtaaa atcaagtttg tcctagttat ctaggacagc cccaaactat ttattaaaaa
      121 actggaacat cctaaatcaa ggcaacacaa gacaatttaa aattccatgt ttgtgtactg
      181 aaatgcttca aaacaccaaa ccaagtttcg ttttctaaaa tacgaaacca tgactgctgc
      241 ctaaattttt ttggattgct aaattatggc agtatgattt tggattttaa attgaaaggc
      301 aagaaaaatg tcaaaaaatg atgtagttaa agtaattggt atattccccc tattgtccga
      361 acaatagagc agacttcccg gcaggtcaaa ttgttttaaa acacggcgaa cacaaaaagg
      421 ttaatcacgg ctttggtgtg attcacattt tggcagaaca caaagcagat ttaacgcggt
      481 ttggattaag ccacgatgaa acaggtgtaa cccttttatg tggaaatgat ttttcaatca
      541 ggcgcaaaaa tttttagtga atttagtgat gtaaggggca atcatcgtcc tatgattgtc
      601 tattctcgga ttgggacagt cgttttagaa cgcaaagaag ttgaggggga aagtgtgtat
      661 tcagttgtta ccgcgtttgg tcgaacaagc cctcgtggta cacaaattgg tacaatgcca
      721 taaaaagaca aaagcaccca ataatttcag gtgctttgtg tagtagccgc agcctctcct
      781 tagcttgata gggccggata ccaatctgac gattgagcta ctgaaattgg ttagcgacaa
      841 ccaacctgcc cacatcagaa cgcccgttcg ctggtttgta cgtcctgaaa aagctcttgc
      901 attaagttaa tcataatggg aaatttaaat tttttcaatg cttacttaaa caaaagcccc
      961 actccaccat taggagtttc tttttcagta tacaagtaaa tattcagtat acaagtaaat
     1021 atttttaaaa tattgattta atttaaaata aaatacttgc aaaaaaagta ttaaattaaa
     1081 cttaagaaag gttaattctg atttacattt ccaaccatac ttctttacag gagaaaatca
     1141 tgaaagagtt acacacctct gaattagttg aagtgtcagg tggcaaattc catatctttg
     1201 cacagggtgg cggcaaccta ggtaaaaaag atatggttgc tgttggtaaa attggtgctt
     1261 cctattcccc taacaatagt ggagtagagt tttctgttag caagcaattt ggatatgtac
     1321 aaggtcttgg tgtacagttt tcgaaaccta cttttggtat tagtaaaaaa tggtaagatt
     1381 ttttgtttta tcctttctga cattaataaa tctatgctca ttaagcgcat gcaatagcca
     1441 ctttacagga aatatcaatc cattaggtac tcacaataaa gttgctaatc ccaattgtgc
     1501 caatagtgcc aatagtcata tcagacaacc cagtaggaaa aactatgatc caactgaata
     1561 tagtgcttgg ttacagtata tgcatgattg caaataatga gtaacgatga aaatttactt
     1621 ttttctcaac cacacttaac aaaggtgaat attatgcaag ttttgacttt gaatgaaatt
     1681 taacaagttt ctggtgctgc ttgtaactgg cgtgatttct caaaaaatac cattggtagt
     1741 gcattaggtg gagcagctgg tggggcaatt gttggttcat ttgcaggtgg tattggtgct
     1801 attccaagtg cgaaattcgg agctattggt ggtgcaatca ctggtgctgt acaatatgga
     1861 agcacttgtt ggtggtaata ttccttaata aaactagggt attttgatat tttctattca
     1921 aaatacccta gtttttcata agaacttaaa tacaaaaagg aacaaataat gaaaaaatat
     1981 agtgattatt ttaaatattt aatctttttt tgattttact cccaacaaat tatctcgtat
     2041 ctcattatgt ggtacaaacc tcaatgagta tgttaagcat tttaagttct tctataataa
     2101 caacttgcct ttttttgttt ttacaaattt atcccaatca aagaaacata aataagtaca
     2161 catgtctaac aatcactcat ttttcagacc agaagtcttt gtagctcaac ggaacaagtg
     2221 gacaggacca gtaggctggg ttggcgcaat tggagctggt attttctctg ctgctggcgg
     2281 atacaatatc ggtcgtggca tgatgaagcc ataagataat tacatcatta aggaaaaggt
     2341 aatttcagtt acagcaatat gtattgaagt tacctttttc tatttagatt gaacaatttt
     2401 gaaagagaaa aattatgaat actgaaacca tttacgccac tgtcttttgc attttagctg
     2461 caaccatttc tggattattg gttaaattta atgtaattaa aatagaaaca tcaatcaata
     2521 gcaaatttat gttattaggc ataagtattt aattattggt gtttttctat ccattttttt
     2581 aagaaataat aataaatgtc ccacttattc cgaaaagaag tctttgtagc ccaacaaaat
     2641 aagtggacag gtcaggttat cttgacccgt ccattctctt ttttatttct gactttttgc
     2701 gcttttctca ttgctctgtg tatcattatc tttttgattt ttggtagcta taccaataaa
     2761 acaaccgttg aaggtcaatt acttccaact atgggggtgg ttcgtgttta ctcttccgat
     2821 atcggcacga ttacgcataa atttgttgaa gatggtaact ttgtcaaagc tggcgaacca
     2881 ttgttcaaac tttccacatc gcgttttggc gaaaaaggaa acgtacaagc caaattggca
     2941 gcagaagcca accttaaaaa aactttggca ttacaagaat tggaacgttt aaagcgcatt
     3001 catcaaaatg agcaaaaaaa tgttcataac aacattcatc gtttaaacaa tcaattagag
     3061 aatattaaac agcaaattac agggcaaaat cgtcaaattc gtttagcgga aaaaaccctt
     3121 aacaagaaca agtttttagc cagtcaaggc gcagtatccc aacaagataa gatgaccgcc
     3181 gaaagccatt tattggaaca acgctcacgt ttggagagcc taaaacttga acaaaataat
     3241 gcaatcaggg aacttgatga acagaaaatt acattaagca gcctgcctga acgccataaa
     3301 accgaattga gccaactcaa ccgtgcgatt acggaaatga accaagaaat tttggatttt
     3361 gatttgaaat ccgaacaaac catacgagct agtaaatcag gttatatatc aacaattaat
     3421 gttgatatag ggcaacaagt tgaaccgtct aaattgctgt taagcattgt ccctgaacaa
     3481 actgaattgg tcgccaatct ttacataccc agtaaagctg ttggttttat taaatcgaaa
     3541 gataaagttg ttttacgtta ccaagcgtac ccttaccaaa aatttggaca tgccacagga
     3601 gaaattattt cagttgccag aactgctctc ggtaaacaag agctatcagg tttaggtatc
     3661 attttcacta acccaacctt attaaatgaa cctgcctatc ttgtgaaagt taaattggaa
     3721 aaacaaacga ttaaagcata cggagaaaac aagccgcttc aaattggcat gattttagaa
     3781 gcagatattc tccatgaacg aaaaaaattg tacgaatggg tacttgaccc actttacagc
     3841 atttcaggaa aaatcaatta aaaatggatt atttatcaag actgtccttt ggatttaaca
     3901 aaaagctacc tgtcattctg caaacagaag ttgctgaatg tggtttagca tgcctgacat
     3961 ccatcttgtc ctattatggc tttcacactg atttaagaac gttacgccaa aaatacaccc
     4021 tgtcattaaa gggcgcaaat cttgcagaca tcatgagatt tggcaatgaa atgaatttaa
     4081 cgccacgagc tttgcgttta gagttagatg agctgtcaaa tttacaacta ccctgcattc
     4141 tccattggaa cttaaaccat tttgttgtac tttgttccat ttccaaagac agtatcgtca
     4201 ttatggaccc tgctgtcggt atgcgaaaaa tcaaaatgga cgaagtttca caaaaattca
     4261 cagggattgc cctagaatta ttccccaata cccattttga agagaaaaaa gaaacaaaga
     4321 aaatcaaaat attatctcta ttaagggggg tcaggcttaa aacgctattt aattcaaatg
     4381 cttatattag ctatttcttt ggaagtcttt gcattggtta gtccattctt tatgcaatgg
     4441 gtaatagacc atgtcattgt aactgctgat aaaaatttat tattgaccct tactttggga
     4501 tttggtttac tgactatcct gcaacagtta attagcctgt tacaagcatg ggtaggtatg
     4561 cacctatcta caactcttaa tttacaatgg aaagccaata tatttaaaag gttacttgac
     4621 ttacctaatg actatttcag taaacgacat ttaggagatg tgatttcaag atttggttca
     4681 atagatcata tccaagaaac actaacttct actttttttg ttttagtttt aaatagctta
     4741 atggctgttt ttactttcgt gttaatgaca atttacagca ctcaattatc gctgattgtt
     4801 cttttaacac ttgttttgta catactaatt cgttggcttg catattaccc attaagaaat
     4861 gcaacagaag aaaatattgt tcatgaagcc aaacaaaact catatttcat ggaaaccatt
     4921 cgtggtatcc aatcagttaa attatttgat aaacattatc aaagacatgg cacttggatg
     4981 agcctatttg tgaatacagt caataccaag ctgacaacag ataaactctc tgctttattt
     5041 gaattttcaa ataaactgtt gtttagcatg gaaaatatta tcataattta tcttggtgca
     5101 agcgcaattt tagatggttc atttacagtc ggtgttctga tggctttttt ggcttataaa
     5161 gggcaatttg aaagcagaac agcttctctc gttgaccaat acatccaaat caaaatgtta
     5221 gggcttcatg ctgaacgttt ggctgacatt actttaaatg aaacagaaac tgaaattatt
     5281 aagtataatc atatacctaa attagataat gaacaactgg ttcttaaagt tgaaaacgtc
     5341 tcattcagat atgctgataa tgagccatat ctttttgaaa acattaattt ggaatttaaa
     5401 gataatgaag cagttgtttt aacaggacaa tctggtcggg ggaagtccac tttgttaaac
     5461 attttaacag gtagcctaaa acctgaaact ggtacagtta gtattaatgg gcatgatata
     5521 tatcaagttt ctccatcctt tattagggga ttgagcggga ttgttcgcca agatgatgtc
     5581 ctttttgcag gttctattgg ggaaaatatt tcattttttg atgaaagccc aaatatggag
     5641 ctcattgaac aatgtgcaaa aatggcacaa atacatgacg atatacttaa aatgccaatg
     5701 ggctatgaga ccttgattgg cgatatggga aatatcttat caggtggaca aaagcagaga
     5761 gttatcttgg ctcgtgcatt gtataaacga cccaaaattc tatttttaga cgaagcaagt
     5821 agccatttag atgtagaaaa tgaacaaaaa attaaccata acctaaaaag tcttggtatt
     5881 atgaaaataa tggttgcaca ccgccaagaa acaattcaat cggcagataa aattctgaat
     5941 ttaggttgaa cagaacaaga cttcattttt ctttaacaaa aagtgaagtc ttttttcaaa
     6001 taatttaata gaatacatga aaatagcggt ttaacgttcc atttcccaat catcacgact
     6061 ggctttgtgt tttggcgatt tttcagtttc ctttttctgt tgaatttgtt gttttttctg
     6121 ctcttgttcc catttttggg ctaatttcac ggtctcattt tcagcccatt ccatcacggc
     6181 acaacgatgt agcttttctc cgatatcgcc attaaagcca gctccacgaa cttcaccata
     6241 aattcttgaa tatttttgat tatattcaat ttcttttcca ttttctttaa aggatttctc
     6301 ccacttttca caaacttcat caaaatcttt caaagggata ttttttaagg ggctgtccta
     6361 gataactagg gaaattcaaa ttaagttaga attatcccta tgagaaaaag tcgtctaagc
     6421 cggtataaac aaaataaact cattgaactg tttgtcgcag gtgtaactgc aagaacggca
     6481 gcagagttag taggcg
//
LOCUS       DAWGJV010000116         6478 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWGJV010000116 DAWGJV010000000
VERSION     DAWGJV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.116"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            16..336
                     /locus_tag="WHQ94_001619"
     CDS             16..336
                     /locus_tag="WHQ94_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ2128874.1"
                     /translation="MGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGYDEIK
                     AKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLAML
                     KFNS"
     gene            347..484
                     /locus_tag="WHQ94_001620"
     CDS             347..484
                     /locus_tag="WHQ94_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ2128875.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(644..1981)
                     /gene="ribB"
                     /locus_tag="WHQ94_001621"
     CDS             complement(644..1981)
                     /gene="ribB"
                     /locus_tag="WHQ94_001621"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ2128876.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVCLNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2203..3215)
                     /locus_tag="WHQ94_001622"
                     /pseudo
     CDS             complement(2203..3215)
                     /locus_tag="WHQ94_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3371..3964)
                     /gene="ribA"
                     /locus_tag="WHQ94_001623"
     CDS             complement(3371..3964)
                     /gene="ribA"
                     /locus_tag="WHQ94_001623"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ2128877.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3957..4631)
                     /locus_tag="WHQ94_001624"
     CDS             complement(3957..4631)
                     /locus_tag="WHQ94_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128878.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4785..4869
                     /locus_tag="WHQ94_001625"
     tRNA            4785..4869
                     /locus_tag="WHQ94_001625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4819..4821,aa:Leu,seq:tag)
     gene            5389..6423
                     /gene="purM"
                     /locus_tag="WHQ94_001626"
     CDS             5389..6423
                     /gene="purM"
                     /locus_tag="WHQ94_001626"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ2128879.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDMVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1609 a         1573 c         1691 g         1605 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatt aaagacggca aaaaaaccgt actttcctgc
       61 gaagaagaac aaaaaatctg caatactttc acgaacaaac aggcagtgga agatttcagt
      121 gtggtaatcg gctacgatga aatcaaagcg aagaatcaca gcttgtcggc ggggcagtat
      181 ttcgaggtaa agattgatta tgtggatatt tccgccgacg aatttgcgca aaaaatggcg
      241 ggattttcag cggatttgga taaacttttc gccgaatctg ccgaattgga aaaagagatt
      301 aaggatagat tggcgatgtt gaaattcaat tcataaatac cgatgaatga aaccggtcaa
      361 acttaaaatg ccgtctgaaa gcctttcaga cggcattttg tttggtttgg aaaatactta
      421 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
      481 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      541 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      601 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      661 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      721 ggacgcgcag ttttttgacg ttgaggccgg cgaggatttg cgcaccgatg ccgtagcttt
      781 tgctgtccca tttgtaggct tggtttgcac ctttgggcag ggtgcggtcg agcagggcgg
      841 cgccgtcttc ggtgcggtgc agaaggatga ctacgccgct ttcggcctgt tggatgcgct
      901 cgagtgcttt gggcagcgac catgaatgac gcgggttagc ttggatgaag tccatgacgc
      961 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggagggcgtg cctttgacga
     1021 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
     1081 acggggtttg tacaggcgca ttgcccatgt cttcaagcag gctttcggta cggctgcggt
     1141 attcgatgag gtcggcaatc gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
     1201 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggaggcag
     1261 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
     1321 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
     1381 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1441 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1501 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1561 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1621 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1681 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1741 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1801 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg ttttttaaga
     1861 gggcggagag ctcgcccgta ttgaggcaga cggcttcggc aaaacgggtt tgcaaaccgc
     1921 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1981 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     2041 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     2101 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     2161 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     2221 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     2281 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgcct tcgcgccgaa
     2341 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     2401 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2461 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2521 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
     2581 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
     2641 caattgcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2701 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2761 ctccaccagc gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2821 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2881 ttgttgtaaa tcaaagagaa tcccccccgc gcgttttttg aacgattggt gtcggcccaa
     2941 tagtcgtcgc tgtccaaaaa gattaggtaa tcgccttttg ccgcccggat accggcgttg
     3001 cgggcatccg acagcccgcc gttttcttga tgaatcactt ttatatgcgg atatttgcct
     3061 gcatattcgt cgcaaatctt cccgcagccg tccggcgaac cgtcatcgac caaaatcatt
     3121 tcataatcgg caaaattttc ggcaagcacg gaatccacgc agcagcggag gtatttttcc
     3181 acattgtaaa tagggacgat gatggagaaa atcataaata tcaatccatt atattaagat
     3241 gtttgcgcgt atgcctcaaa cccgcgctcg caatgcgttt gcatccacta taaaacttat
     3301 tgctttatca aggtatggaa acctgtttcc cgaaaggcgg cgcaggatgc ccgttccctg
     3361 caactttgcc ttattccgac atcagatgtc ccagcttgtc tgctttggtt tggaggtagc
     3421 gtttgttttc aaggttttcc ccgacgtgca gggggatgcg ttcgaccacg ttaatccccg
     3481 catctttcag ggtttggatt ttttcggggt tgttggtcag cagtttgacc gagcggatgc
     3541 ccagatattc gtagatagat tgcgccaaac ggaaatcgcg ggcatcgacg ggcagcccga
     3601 gtgccaaatt ggcttcaacc gtatccatac cttggtcttg cagatgatag gcgcggattt
     3661 tgttaatcag cccgatgccg cgtccttcct gacgcagata gacgatgatg ccgcgcccct
     3721 ctgtctgtac cgccctcatg gccgcttcaa gttgaggtcc gcagtcgcat tttctcgaga
     3781 acagcgcatc gcccgtcagg cattcggagt ggatgcgcgt cagaaccgga ttgccgtctg
     3841 aaaaattacc gacggtcagc gcgacgtgtt cctgcccgtt tgcctcttca aagccgtgca
     3901 tcgtaaatac gccccattcg gtcggcaggc ggcaggaagc aacatagttt aataattcag
     3961 acattttcat ctccttctgc ggcagtgatg cccaaaagct gcttcaaagg ttcggacaaa
     4021 gccaaggcaa gagccaccca ttccacctgt gctgccgtac ccgcacactc tgcgcattca
     4081 aattccacat gaaccacgcc caacacaccg ccactttccg tgcagaccgg aatggaaatt
     4141 tgcgccgccg aagcatgatt gcgttctccc gaaagctctc ccaaatccaa ccaacggcgt
     4201 acatccgagg caacattcat ccagccgctt tgcgccgaac ggcaagccag cgaaaccttg
     4261 cctgccgctt catccaaatc ccacaggttt tccaaaacct cgccctgccg ggacaggcaa
     4321 accagccgaa aggcggcatt ttcagacggc atcagggcat aaacggctgc ggcgcgcacg
     4381 cctttacagc gtgaaaacac cgaatccaaa gccatgaaaa gccgtttcag accttcctca
     4441 ttcccgctgt tttgctccaa ataatcggca agcctccaat cctcatgctc aagccacaaa
     4501 accgaacgct caatcgaagt cgtccccata tccattaccg tctgcgccgt cagatacgcc
     4561 gtccgaacct cgtcaagcgg cagcttcaaa ccctgagtca gcagaaaatc cttaatcaaa
     4621 gcagcaggca tacccaaatt ccgtcaataa aaacaaaaaa ccgcccgatt ccgatgtcag
     4681 gcggacgaaa aacagatttt accgccccaa accccaagcc atcaacagcc ccgccgaaaa
     4741 ccttgcacaa aaaaacaaaa tctgtataat tgtccaattc ccatgcggac gtggcgaaat
     4801 tggtagacgc accagattta ggttctggcg ccgagaggtg tgagagttcg agtctctccg
     4861 tccgcaccaa taatgataaa taaatcatat agttaatgct tatcgggaca aattagtgcc
     4921 acaaaacata agtcgtctgc ttttcagcgg gcggcttttt tatctgccta tatgtttcaa
     4981 cacgcagaac ggtacataaa gtgccgccat ttgttctacc ccgaatcaga agcgggtgct
     5041 tccgcccgca atcagattca ccgcccctaa tgccggggtt tcaactggaa agaagaaaat
     5101 attgattttg atgatggcat caaaaaaccc cttccgcccg tctgccaaaa ggaatggtta
     5161 agaaaagcaa tgcgtctttt gatggaacgc gatatataag gaattgaggg gaagggcagg
     5221 cgggtttctg cccgttaatc ctgcggacga tcccgccaaa cccgacacgc gcgggcatat
     5281 ggcggattgt ttacaatatt gtctaaaaat ctgaacctgc aaacgtgaaa taaggtagaa
     5341 tacgccccgt tattttaccg tccaatcccc atttaccaag gaaaaacgat gagtacttca
     5401 ttgagttacc gcgatgcagg tgtcgatatc gacgcaggcg accaactggt cgaaaacatc
     5461 aaaccgtttg ccaaacgcac catgcgtcca gaagtattgg gggatttggg cggttttggc
     5521 gcattggtcg aaatcggcaa gaaatataaa aatcccgtat tggtcagcgg tacggacggt
     5581 gtgggtacca agctcaagct tgcctttgat tgggataaac acgatatggt gggcatcgac
     5641 cttgttgcaa tgagtgtcaa cgatattttg gttcaaggag cagaacctct gtttttcttg
     5701 gattatttcg cctgcggcaa attggatgtt ccgcgcgcaa ccgatgtcat caaaggcatt
     5761 gcacaaggtt gcgaagaatc cggctgcgcc ctgattggcg gggaaactgc cgaaatgccg
     5821 ggcatgtatc ccgtcggaga atacgatttg gcgggttttg ccgtcggcgt agtggaaaaa
     5881 gagaatgtca ttaccggccg cagtatcggc gcgggcgatg tggtattggg tttggcttcc
     5941 aacggcgcac attcaaacgg ctattccctt atccgtaaaa tcatcgaacg cgacaatccc
     6001 gatttggatg ccgagtttga taatggcaaa accttgcggg aggctgttat tgcgccgacc
     6061 cgtctgtatg tgaaaccgat tcttgccgct ttggaaaaat ttaccattaa aggtatggca
     6121 cacattaccg gcggcggcat taccgaaaac gtgccgcgcg tgttgcctga aaacacggtt
     6181 gcacaaatcg atgctaaatc gtgggaattg cccaagctct tccaatggct tcaaaaggcg
     6241 ggcaatgtgg aaacccaaga aatgtaccga acctttaact gcggcatcgg catggtcgtt
     6301 attgttgctg ccgaagatgc cgatgcggtt cagggtctct tgggtgaaca aggtgaaaca
     6361 gtttaccgtt taggtttgat ccgtgagcgt cagggagacg agcatcaaac ccaggttgcc
     6421 tgattgcttc tatagcgaaa tgccgtctca aacatgggtt tggacggcat ttttatag
//
LOCUS       DAWGJV010000117         6434 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWGJV010000117 DAWGJV010000000
VERSION     DAWGJV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.117"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..488
                     /locus_tag="WHQ94_001627"
     CDS             <1..488
                     /locus_tag="WHQ94_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor family protein"
                     /protein_id="HEZ2128880.1"
                     /translation="DILINQGSYFSGAFPICQKFKNMPNPERFDYRKKVANLTFQHKL
                     RYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLKESLKFGFIH
                     EQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLDRMYDLHANQ
                     N"
     gene            509..820
                     /locus_tag="WHQ94_001628"
     CDS             509..820
                     /locus_tag="WHQ94_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128881.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            824..1024
                     /locus_tag="WHQ94_001629"
     CDS             824..1024
                     /locus_tag="WHQ94_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128882.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            1251..1481
                     /locus_tag="WHQ94_001630"
     CDS             1251..1481
                     /locus_tag="WHQ94_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128883.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
     gene            1509..1808
                     /locus_tag="WHQ94_001631"
     CDS             1509..1808
                     /locus_tag="WHQ94_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128884.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            1927..2247
                     /locus_tag="WHQ94_001632"
     CDS             1927..2247
                     /locus_tag="WHQ94_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ2128885.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            2231..3742
                     /locus_tag="WHQ94_001633"
     CDS             2231..3742
                     /locus_tag="WHQ94_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ2128886.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAGVLAGVGALARQGAKFSTRAVPYVGTALLAY
                     DIYETFKDDIQAQGYQYDPETDKFVKGYEYSNCIWEHAENGIKTYGCYGVDSSIMRLM
                     SDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCV
                     VNKGDDYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKV
                     EVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQ
                     PLPEVSPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRT
                     NGRDGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQNSAQCPA
                     PVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            4046..4330
                     /locus_tag="WHQ94_001634"
     CDS             4046..4330
                     /locus_tag="WHQ94_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ2128887.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            4331..5434
                     /locus_tag="WHQ94_001635"
     CDS             4331..5434
                     /locus_tag="WHQ94_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ2128888.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCACYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKP"
     gene            5733..>6434
                     /locus_tag="WHQ94_001636"
     CDS             5733..>6434
                     /locus_tag="WHQ94_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HEZ2128889.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQ"
BASE COUNT         2068 a         1327 c         1445 g         1594 t
ORIGIN      
        1 tggatattct gataaatcaa ggttcttact tttcaggcgc tttcccgatt tgtcagaaat
       61 ttaaaaatat gccgaatccg gaaaggttcg attaccgtaa aaaagtggct aatttaactt
      121 ttcagcataa attgagatac gcaaaaaacg cggtcggcaa actgattaat ttcatgtttg
      181 atatgggttt tgatagtgat gaaattgtca gatatctgaa ggcagatttg gggtatccca
      241 aagggctaga acctgaaaaa tattcgttgg ccggattgaa ggaatctttg aaattcggct
      301 ttatccacga acaaccggat gtagatttag aggttgaatt ggaagaactc ggaattatca
      361 aatttaagca atcagataaa ttcgatccgg ataaaaggct tttcgatcca catcacgatg
      421 tagaaagtga gaggcaatat cagctttatc tcgacagaat gtatgatctt catgcaaatc
      481 aaaattaacc taaaaaggaa aaattaatat gtttaatcaa actcaaactg taacttatcc
      541 cgcaactttt ttaggagcta aaaaattcaa aggcgaaatt gatggctcta atatcgacac
      601 ttgttccgta ttggttgcaa cacctttgcc ggcacagtcg ggaaatgctg ttggattcac
      661 ggcagcacaa atgaagttcg gggacagtaa gaatttctca aaattagaga atctcaaata
      721 cccgtgcgaa gttatggtaa cggttgaaat gacttcgaca ggtaagggca tggttccttc
      781 attaattgat tttcaggtgg cagaaaagcc gaaaggttga tttatgaaat ttgaagaacg
      841 tttcatagtt caagacttgg aaacgcatga ctttatttat cccgatcctt tcggtgatgt
      901 ggggtttact caaaatatta aatcagcagg tcaatttgaa agctacgaag atgcgttgaa
      961 ttcaggcata aatgaaatag gcggaggatt ccagatattt cagttcttcg taaaatcgga
     1021 ataaaagaaa aacaggctcg gcgggcggtc tgtcaacctt tcacaaagcc cgcaaaaagg
     1081 attaaaaatg aataaagcaa ctttaattat cggatttttt gtttgcctca ttcttttcat
     1141 tttctcaatt ctctatttca taaattaaaa acaaaattcg ggagcaggct tcgcccggat
     1201 ttaaacctaa gaagccgtat tactttaaat atcaaaaaag gaaaaaaacg atgaacatcg
     1261 ttaaaaaata cgctgtaaaa gcagccttgg cagccggtat cttcacaccg gccattgtta
     1321 tggcagatac ctttgatgca tccgcgattg gtacgcaagt agcgaatgta atcatgggtt
     1381 tcgtgtcaat ggtttccgcc gtgggtatgg cggccattac cgtgattctt gcaatccaag
     1441 gcttcaaaat ggcttggagc atgattaaat ctgtcagata aacagagtga agaaaaaggg
     1501 gcgtataaat gggctatcgt gtcggcataa attgttttga tacaagattg caggcagacg
     1561 actatttatt gtcgtccctt cctcctactg ttacccagga cggaaaaatc atcaggccgg
     1621 aaagggtggg cgataaatgg attttgaacg gaaagccggt cacgctgtct tatccgaaat
     1681 gttcgaatta cgaacaagtt aaatccggag cttatctcgg gtctatggtt ttaattctgt
     1741 ttgtcgttat ttacggcttc aggctgctga ttaatttctt aaaagacata ggcaaagtag
     1801 gggcgtgatg atgtttgtcg atttttggtt tttgctcgga tttttcctgg ctttgtctgt
     1861 cgctttgata tttatatgac gtgttttaaa atcaggcttt caaaacaacc tttgaaagac
     1921 agaaacatga acaagccgtt tatcacgcag gcgcagttgg cactttataa atatcagccg
     1981 tccagcaagt attttgggca gtcgatggca ttaattgcgt ctaaggaatt tgaagagttt
     2041 gtaagaaatg taaaagaata cgacgtaata gaatgtttct cttatttttt aaataagagg
     2101 gtaacgcata atatttggaa aatttatttt tctgatgagt ctaatatttt tattaggaag
     2161 tcagaagaaa atggaaaaat ttcgcatgaa tttatttact cggaattttc tgatagcaac
     2221 accgatttta atgtgttgtt ctcttagttt tgcagaacca gcaagaatag atgatcgaat
     2281 aataaaattt aggccatcta aatcaaagtt ttttgaatct acaggatata gaaaaatcaa
     2341 taatgaattt tctaaattca cagaagcggc aaatgtcgaa catatcccca cgggcgcaaa
     2401 agcccgaatc aacgcaaaga taaccgccag cgtatccaga gcaggagtct tggcaggcgt
     2461 tggcgcatta gcccgccaag gcgcgaaatt cagcacaagg gcagtccctt atgtagggac
     2521 tgcactttta gcctacgaca tatacgaaac tttcaaggac gacattcaag cccaaggcta
     2581 ccaatacgat cccgaaaccg acaaatttgt aaaaggctac gaatatagta attgcatttg
     2641 ggagcacgcc gaaaacggta ttaaaaccta tggctgctac ggcgttgaca gctcaatcat
     2701 gcgtcttatg tccgattaca gcagattccc cgaagtcaaa gaattgatgg aaagccaaat
     2761 gtataggctg gcacgtccgt tttggaattg gcataaagaa gaactgaata aattaagttc
     2821 tttggattgg aataattttg ttttaaatcg ttgcacattt gattggaacg gcggagattg
     2881 tgtggtcaat aaaggtgatg attacagaaa tggggctaat ttttccctta gccgcaatcc
     2941 gaaatacaaa gaagaaatgg atgccaaaaa gctggaagag attttatcgt tgaaagtcga
     3001 tgccaatccc gacaaataca taaaggcaac cggttatccc ggttattccg aaaaagtaga
     3061 agtcgcaccc ggaacaaaag tgaatatggg tcccgtcacg gacaggaacg ggaatcccgt
     3121 tcaggttgtc gcaacattcg gcagggattc gcaaggcaac accacagtgg atgttcaagt
     3181 aatcccgcgt cccgacctca cacccggaag cgcggaagca ccgaacgcac agccgctgcc
     3241 cgaagtatcg cccgccgaaa accccgcaaa caacccgaac cccaatgaga accccggcac
     3301 gagccccaat cccgaacccg accccgattt gaatcccgat gcaaatcccg atacggacgg
     3361 acagcccggc acaagacccg attcccccgc agttccgggc cgcacaaacg gcagggacgg
     3421 cgaagacggc ggccttttgt gcaaattctt ccccgacatt ctcgcttgcg acaggctgcc
     3481 cgagcccaat ccggcagaag atttaaatct gccgtctgaa accgtcaatg tagagtttca
     3541 gaaatcagga atctttcaaa attccgcaca gtgtcccgca cctgtcactt tcacagtgac
     3601 tgtgcttgat tccagcaggc agttcgcgtt cagctttgag aacgcatgta ccatagccga
     3661 acggctaagg tacatgcttc tcgcccttgc ttgggcggtt gccgcatttt tctgtatccg
     3721 cacagtatcc cgtgaagtct agcaggcgca gcaccgccgg gcttcagtaa cttgtaccaa
     3781 ggcaggagga ggacgtccag aaagatttgt aaagacggct ttatcgtctt tataaatctt
     3841 tttggatacc ccttgccgcc ccgccaaaag aatacactct gccgcaaggg caggtggtaa
     3901 ggcgcgcgct ttttgcgccg ttccccctgc ccccgcggcg tcgcaagtga gactgggggt
     3961 gtgggggcta gtccccgcaa aatctttcag attaagaaac atttttttaa tgaggcaacc
     4021 gtgcctttta agaaagggat agcaaatgaa attgttggcc gcattgattc cgctgttgat
     4081 gagcgtcgca ggccgtatat tgactgcatt aggcttgatg gctgttactt attcgggtgt
     4141 agatagattg gtagcccatt ttcagcaggc gataaccagt agcataacgg gcgcgcctca
     4201 agcgatgttg cagctctttt atataagcgg tggcggcacc gttcttaata tcctgtttgg
     4261 cgcgatcgcc tttattctgt cattcaaaca aatgacaaaa ctagcaacct caatcgggaa
     4321 gaaaaaataa atggcagaga tctgtttgat aaccggcacg cccggttcag ggaaaacatt
     4381 aaaaatggtt tccatgatgg cgaatgatga aatgtttaag cctgatgaaa acggcatacg
     4441 ccgtaaagta tttacgaaca taaaaggctt gaaaataccg cacacctaca tagaaacgga
     4501 cgcaaaaaag ctgccgaaat cgacagatga gcagctatcg gcgcatgata tgtacgaatg
     4561 gataaagaag cccgaaaata tcgggtctat tgtcattgta gatgaagctc aagacgtatg
     4621 gccggcacgc tcggcaggtt caaaaatccc tgaaaatgtc caatggctga atacgcacag
     4681 acatcagggc attgatatat ttgttttgac tcaaggtcct aagcttctag atcaaaatct
     4741 tagaacgctt gtacggaaac attaccacat cgcttcaaac aagatgggta tgcgtacgct
     4801 tttagaatgg aaaatatgcg cggacgatcc cgtaaaaatg gcatcaagcg cattctccag
     4861 tatctataca ctggataaaa aagtttatga cttgtacgaa tcagcggaag ttcataccgt
     4921 aaataaggtc aagcggtcaa agtggtttta cactctgcca gtaatagtat tgctgattcc
     4981 cgtgtttgtc ggcctgtcct ataaaatgtt gagcagttac ggaaaaaaac aggaagaacc
     5041 cgcagcacaa gaatcggcgg caacagaaca gcaggcagta cttccggata aaacagaagg
     5101 cgagccggta aataacggca accttaccgc agatatgttt gttccgacat tgtccgaaaa
     5161 acccgaaagc aagccgattt ataacggtgt aaggcaggta agaacctttg aatatatagc
     5221 aggctgtata gaaggcggaa gaaccggatg cgcctgctat tcgcatcaag ggacggcatt
     5281 gaaagaagtg acggagttga tgtgcaagga ctatgtaaaa aacggcttgc cgtttaaccc
     5341 atacaaagaa gaaagccaag ggcaggaagt tcagcaaagc gcgcagcaac attcggacag
     5401 ggcgcaagtt gccacattgg gcggaaaacc gtagcagaac ctaatgtacg ataattggga
     5461 agaacgcggg aaaccgtttg aaggaatcgg cgggggcgtg gtcggatcgg caaactgaag
     5521 aaaacggcaa gagagaaaaa agacccgtaa accgtttgaa tatagacggt ttacgggtct
     5581 ttgtttcgcg caaagcaagg gctaaggcag tcaggcagca aatcccgcaa tgtattaaaa
     5641 cagacgcgta gaaatgccgg ctgcctttat ccatcctcga aattgaatat catcctagcc
     5701 gtatcaaggc tgtataaata aggaaaatac caatgaatat aatcgggctg gacatctcaa
     5761 aggacaccat agacgcaaca ttgcataaaa caaacggaag tatccattac attaaattta
     5821 agaataatga tgatggatta aaacagttta gattgtggat aaagggaaac agaatcagaa
     5881 aagtctatat cggcatggag gcaacaggca tctattacga aaaggcagca gatatgcttt
     5941 cttcctacta tactgtttac gttattaatc ccttaaaaat caaggactac ggaaaaagca
     6001 ggtttaaccg taccaaaacc gacaaagcag attcaaacct gatagcagac tacataaaaa
     6061 ggcatcaaga tacattgata ccgtatcaga tacccaaaaa caaagcactg caaaaactga
     6121 ttaatcttaa aaaccaatta cagcaacagc agaagcaaat taaaaaccgt cttcatagca
     6181 ctgaagaaga cttcataagg aacatacatc aagacttgat agataccata caggacaaga
     6241 tggaacaggt aaaaatagcc atatccgaac aaatcaaaaa acaaacggac tataaccatt
     6301 accgcaatct tcaaaccatc ccgagcatag gcaaagacac cgcatcagtt ctttatgcgc
     6361 aactgacaga aaaacatttt aaaaccgcaa accagtttgt atcctatgcc ggattaagtc
     6421 ccgccatcat acaa
//
LOCUS       DAWGJV010000118         6302 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWGJV010000118 DAWGJV010000000
VERSION     DAWGJV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.118"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(506..751)
                     /locus_tag="WHQ94_001637"
     CDS             complement(506..751)
                     /locus_tag="WHQ94_001637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128890.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="WHQ94_001638"
     CDS             complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="WHQ94_001638"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ2128891.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1416..3311)
                     /gene="mnmG"
                     /locus_tag="WHQ94_001639"
     CDS             complement(1416..3311)
                     /gene="mnmG"
                     /locus_tag="WHQ94_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ2128892.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3636..5063)
                     /locus_tag="WHQ94_001640"
     CDS             complement(3636..5063)
                     /locus_tag="WHQ94_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2128893.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5223..6212
                     /gene="pdxA"
                     /locus_tag="WHQ94_001641"
     CDS             5223..6212
                     /gene="pdxA"
                     /locus_tag="WHQ94_001641"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ2128894.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1330 a         1660 c         1807 g         1505 t
ORIGIN      
        1 tgatttttgt taatccacta tatcgtcatt cccgcaaaaa caaaaaaatc aaaaacacaa
       61 aactgaaata tcgtcattcc cgtgcaggcg ggaatctagg tctgtcggta cggaaactta
      121 tcgggaaaaa cggtttttcc aaccctgaga ctccggattc ctgttttcgc gggaatccgg
      181 ttttttgagt ttcagtcatt tttgataaat tcttgcagct ttgagtttct agattcccgc
      241 ttttgcggga atgacgcgga aaagttgctg tgatttcgga taaattttcg tcacgcttaa
      301 tttctgtttt atccgataaa tgcctgcaat ctaaaatttc gtcattcccg caaaaacaaa
      361 aaatcaaaac agaagcctaa aatttcgtca ttcccgcgaa ggcgggaatc taggtctgtc
      421 ggtacggaaa cttatcgggt aaaacggttt tgccagccct gagactccgg attcctgttt
      481 tcgtaggaat ccggtttttt gagcttcagt catttttgat aaattcttgc agctttgagt
      541 ttctagattc ccgcttttgc gggaatggcg gtttggaagt tacctgaaat tcaaaaaaaa
      601 cggaaaccgg acggattgga ttcccgcctg cgcgggaatg acggatttta ggtttctttt
      661 ttgattttct gtttttcgcg ggaatggcgg tatgggttct ttctctttga agttgcgatg
      721 ctggaaatgc cgtctgaagt tcagacggca tttttgtgcc ggtttaaaac agcgtctgct
      781 gcgcgagcag gtttctgacg ggggcgaagt cgcggcgatg ttcgggcagc acgccgtatt
      841 gtttgagggc ttccagatgc tgcttcgtgc cgtaaccttt gtgtttgtcg aaaccgtatt
      901 ggggatggcg ttgcgccagt gcgtacattt ccgcatcgcg tgcggtcttt gccaaaacgg
      961 atgcggcgga gatttcgatg attttgctgt cgcctttgac gacggcttcg gcagggatgt
     1021 tcaaatgttc gggaatgcgg ttgccgtcga tgaatattct ttcgggacgc acagccaaac
     1081 cgtcaacggc gcgtttcatc gcgagcatgg tggcgtgcag gatgttgagg ctggcgattt
     1141 cttcgggcga ggcggcggca acgtgccacg caaccgcctg attttttatc atttcggcaa
     1201 gcgcgtcgcg ttttttctcg ctgagttttt tggagtcggt cagtccgggc aggtcgaata
     1261 tttccggaag gatgacggcg gcggcaaaca cgctgccgac taaaggcccg cgccctgcct
     1321 cgtccacgcc ggcggtcagt atgtgcatga tgtttcctgt cgggatggcg cgatgccgtc
     1381 tgaacggtgt ttcagacggc atcgcgccga tgtgtttatt tcgcgtcttt aaacccgcgc
     1441 ttcaaatgca ccatcagcaa tgccactgcc gcaggggtta cgccggaaat gcggcttgcc
     1501 tgcccgacgg tttcgggttt gtgctggttg agcttttgct gcacttctgc cgacaaacct
     1561 ttaactttgc tgtaatcgat atcctcgggc agctttaagg tttcgatgtc gcggcggctg
     1621 tcgatttctt cgttttggcg gtcgatatag ccttggtatt tgacttggat ttcgacttgt
     1681 tcgaccactt cggcggagag gcgttcagac ggcatcgcgc cttcgagcgt catcagcgcg
     1741 gcgtagtcga ggttcgggcg gcgcaggagg tcgtgcaggt tggcttcgcg gctgagcttt
     1801 tgcccgaata cgcggatttg ttcgtcttcg gcgagttttt gcggcgtgta ccacgttgtt
     1861 ttcaaacgtt ggatttcgcg ttcgatggct tcgcgttttt cgttgaacat gcgccattgc
     1921 gcttcaccca ccaagccgat tttgtagccg tcttcggtca ggcgcatatc ggcgttgtct
     1981 tccctgagtt gcaggcggta ttcggcacgg ctggtgaaca ttcggtaggg ttcgttcacg
     2041 cctttggtga tgaggtcgtc caccaatacg ccgagatagg cttgttcgcg gcgcagcagg
     2101 agcgggtctt gttcgcggac gtattgcacg gcgttcgcgc ctgccagcag tccttgcgcg
     2161 gcggcttctt cataacctgt cgtaccgttg atttgcccgg cgaaaaacaa tccggcaatg
     2221 gttttggttt cgaggcttgc tttgaggttg cgcggatcga agtagtcgta ttcgatggcg
     2281 tagccggggc gcaggatatg ggcgttttcc aaacctttca tactgcggac gagcgcgatt
     2341 tggatgtcga acggcaggct ggtggagata ccgttggggt agtattcgtg cgtagtcagg
     2401 ccttcgggtt ctaggaaaat ctggtggctg tctttgtcgg cgaagcggtt gattttgtct
     2461 tcgatagacg gacaataacg cgggcccacg ccttcgattt tgccggtaaa catcgggctg
     2521 cggtcgaagc ctgagcggat gatgtcgtgg gtttgcgtgt tggtatgcgt aatccagcag
     2581 gacacttggc gcgggtgcat ttcggcgttg ccgcgtacgg acatgacggg aacgggcgtg
     2641 tcgccgggct gttcggtcag ttgggagaag tcaatcgtgc gtccgtcgat gcgcggcggc
     2701 gtgccggttt tcagacggcc ttgcggcagg ttcaattcgc gcaaacgtcc gcccagcgat
     2761 ttggcggcgg ggtcgccggc gcgtccgccc tcgtagtttt ccaaaccgat gtggattttg
     2821 ccggacaaaa acgtgcctgc ggtcaacacc acggcgcgtg ctttaaactc cacgcccatc
     2881 gcggtgatta cgcctgaaat gcggtcgcca tcgagcgtta cgtcttcgac ggcttgttgg
     2941 aaaaggtcga ggttttcttg gttttccaac atttcgcgga tggcggcttt atataggata
     3001 cgatccgcct gcgcgcgcgt ggcccgtact gccgcgcctt tgctggcgtt cagacggcgg
     3061 aactggatgc cggatttgtc ggttgccaac gccatcgcgc cgccgagcgc gtcgagttcg
     3121 cgcaccaaat gccctttgcc gatgccgccg atggaagggt tgcacgacat ttgtccgagc
     3181 gtttcaatat tgtgtgtgag caaaagcgtc tgcgcgccca tacgggcggc ggcgagcgcg
     3241 gcttctgtgc cggcgtgtcc gccgccgacg acgataacgt cgtaggtttt ggggtaaatc
     3301 atgtgggtca tgtgtgtatt gcctgacggt gtttcagacg gcatttatag tggattaaca
     3361 aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gtaccaagtg aatcggttct gtactatctg tactgtctgc ggcttcgccg ccttgtcccg
     3481 atttttgtta aaccactata ttcaatatgc cgtctgaaaa acgaaatgga ttcaaaagta
     3541 aagggttggg attgtacgct tgttcgccct gtttttacag tgtgcggaaa gggaaaagcc
     3601 gcttcgcggg gaagcggctc cggtaagggc gggatttacc aaacgtcgga tttgatacgg
     3661 cgtttcaggc ccggatgttc ggaaagtttg aactcggggt ctttgcccat tttcagcttg
     3721 gcggtgtaat cgcgcagcag cataaacgcc agtggcgaga gcagcaggat ggcgacaagg
     3781 ttgatccacg ccataatgcc catcgccata tccgccatat cccagaccaa aggcacattg
     3841 gcaaccgcgc cgaaatagac ccacgccaaa accagcatac ggaaaacggc ggtaatcagc
     3901 caatggcttt tgatgaattg gacgttggac tcggcatagg catagttgcc gataacggtg
     3961 gaaaaggcaa acataaacag gatgacggcg aggaagcccg cgccccattg ccccacttgg
     4021 ctgacaatcg ccgcctgcgt cagcgccgca ccgctcaaat cgccgtaagg ctgttggtaa
     4081 atcaagatga tgaaggcggt gcaagaacaa acgatgatgg tatcgacaaa cacgcccagc
     4141 atttgaatca taccttgcga aacagggtgt ttcacttcgg cggcggcggc ggcgttcggc
     4201 gcggaaccca tacccgcctc gttggaatac aggccgcgtt tgatgcccat catcatcgtt
     4261 tgcgaaatca gaccgccgag taagccgcct gctgccgcgt cgaatttgaa cgcgcccgaa
     4321 aaaatctgac cgaacacgtc cggaatcatc ggaatattgg tcaaaatgat gaaaagcgcg
     4381 ataaagaggt acaaaaccgc catcaggggg acgacgattt ccgccgcttt agatatgcgc
     4441 ctgatgccgc cgaagataat cggcgcggtt aaaatcacca gggcgacgcc gacataatga
     4501 ggctcccaac cccacgccgc tttgacggta tcggcaatgg tattggtctg aaccgcttca
     4561 aacacaaagc cgaaacagaa aatcaggctc agggcgaaca acacgcccag ccatttctgc
     4621 cccagccctt gagtgatgta gtaggcaggg ccgccccgga aatggtggtt gtcgtagtcg
     4681 cggactttaa agagctgcgc cagcgaagat tcgacaaacg ccgaactcat accgattaag
     4741 gcggttaccc acatccaaaa caccgcgccc ggtccgccga ctttgatggc gatggccacg
     4801 cccgcgatat tgcccacgcc cacgcggctg gcaaggccgg ttacaaatgc ctgaaacggc
     4861 gtgatgccgt gagggtcgtc cccctgtttg cggccgccga gcatttcttt gatgctgcgc
     4921 ccgaacaggc ggaattggac aaagcccgtg gttacggtga agaaaagccc cgtacccaaa
     4981 agcatataaa ccaagtatga ccacatcgga tcgttgatgg cgccgaccca gccgtgcagc
     5041 cattcggtaa agttctcgtt catatcgctt ccttaaagtt gaaactcgca catattggcg
     5101 gtatgcaagc agggtttaaa ttttgtaaac gcccattcta gcagattgtc aacaaaatca
     5161 gaaaaattta catcgccgcg cggctgcggc gttagaatcg cattttgttt ggagcaaaca
     5221 cgatgaaaca gcctgttttt gccgttactt ccggcgagcc tgccggcatc ggccccgata
     5281 tttgtttgga cttggcgttt gcacgcctgc cctgccgctg cgcggtattg ggcgacaaaa
     5341 acctattgcg cgcgcgcgcc gaagccttgg gcaaaagcgt cgtcctgcgc gacttcgatc
     5401 cagaatcagg cggcgcggca tacggcgagc tggaagtgct gcacatccct gccgtcgaag
     5461 cggttgaggc gggcaaactc aatcccgcca acgccgccta tgtgctgcaa cttttggaca
     5521 ccgcgctcgc aggcatttca gacggcattt tcgacggcat cgttaccgcg ccgctgcaca
     5581 aaggcatcat caacgacgcg cgcgcaagca caggtttttt cagcggacac accgaatatc
     5641 tggcggaaaa aagcggcacg gggcaggtcg tgatgatgct tgccggcaaa ggcctgcgcg
     5701 tcgccctcgt aacgacccac ctgccgctga aagacgttgc cgccgccatc acgcaaccgc
     5761 tgattgaaag cgtcgcacgc attttgcatc acgacttaaa acacaaattc ggcatcaaaa
     5821 atcccaaaat ccttgtcgcc ggacttaatc cccacgccgg cgaaggcgga cacctcggac
     5881 acgaagaaac cgacaccatt atccctgcat tggaaaacct gcgccgcgaa gggataaacc
     5941 ttgccggccc gtatccggcg gacacattgt tccagccgtt tatgctcgaa ggtgcggatg
     6001 ccgtattggc gatgtaccac gaccaagggc tgcccgtgtt gaaataccac agtttcgggc
     6061 agggcgtgaa catcacgctg ggtctgccct ttatccgcac ctctgtcgat cacggcaccg
     6121 cgctcgattt ggcggcaacc ggcagggcgg attccggcag cctgataact gccgtggaga
     6181 ccgccgtcga gatggcgcgc ggcagccttt aaagatgata aaagacccgt catttccgcg
     6241 caggcggcat cgtccaccct ccgccgtcat tcccgcgcag gcgggaatcc agaccttcgg
     6301 gc
//
LOCUS       DAWGJV010000119         6266 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWGJV010000119 DAWGJV010000000
VERSION     DAWGJV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.119"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(84..845)
                     /locus_tag="WHQ94_001642"
     CDS             complement(84..845)
                     /locus_tag="WHQ94_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128895.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIEGGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            945..1196
                     /locus_tag="WHQ94_001643"
     CDS             945..1196
                     /locus_tag="WHQ94_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128896.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     LESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1248..2054
                     /locus_tag="WHQ94_001644"
     CDS             1248..2054
                     /locus_tag="WHQ94_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ2128897.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            2361..3884
                     /locus_tag="WHQ94_001645"
     CDS             2361..3884
                     /locus_tag="WHQ94_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ2128898.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            3982..5394
                     /gene="trkA"
                     /locus_tag="WHQ94_001646"
     CDS             3982..5394
                     /gene="trkA"
                     /locus_tag="WHQ94_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ2128899.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(5879..5992)
                     /locus_tag="WHQ94_001647"
                     /pseudo
     CDS             complement(5879..5992)
                     /locus_tag="WHQ94_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            6133..6208
                     /locus_tag="WHQ94_001648"
     tRNA            6133..6208
                     /locus_tag="WHQ94_001648"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6167..6169,aa:Glu,seq:ttc)
BASE COUNT         1574 a         1854 c         1514 g         1324 t
ORIGIN      
        1 atattttgcg cctgttccat gaggctttca agtcggatga gaatgcaaat gccgtctgaa
       61 acggctttca gacggcatgg caatcagcgt ttgtatttta actcgtactt gatgtcgttg
      121 aggattttgc ggacatcgtg ttccaacacg tcttcgacta ccgcccccgc ctgctcgtgc
      181 agcatctgct ggagctgata ggtgaaaacc gccatctgct tttgcaccgc cgttcggatg
      241 atgccgttga cggtatcggt cagatggggg cgcaggcgtt tgatcagccg ttcggtcagc
      301 tcctgttcgg acaggcagaa cacttcgcgc cggttgacgg ctttcgggtt caggatattg
      361 agttggacgg gcatcaacgt ttcttccgca tcgttttccc cgttttccga aaccgccggc
      421 tcattcgtgc cggattctgc ctcgtcggcg ttttccccgc cttcaatctg tccggtttca
      481 aattcgacac tgtctttttt ggtatcaaac cggattctcc gccgcgattc gatgtgtttt
      541 tccgaaaccg acatttgcag ggaagcctgc gcgttgagcc agttttcctg aaggacgatc
      601 atcgggtcgg tttcgatttc ttccccgcaa tcggcaacgg cggcattgtg ctcctcctgc
      661 catttcttca gatacgcctt taacacacgg tttcggcttg catcgtccag tttcggcaca
      721 gacgcgtccg ttccggtttc agaagggcgg gacagcggcg cgtaagtcgg cactgccttc
      781 atacggcgcg tctgacgcag gttttccaaa cgtttttccc aattcggctc tttattcgca
      841 tccattttcg gcttccggtc cttaatcttt gcaagcagac aaacccgcgc ccaaagcgcg
      901 gtttgatata atggcgcatt ttaacagatt cgcgaggata catcatgggc agcatcgaac
      961 agcgtttgga atatctggaa gaggcgaacg acgtgctgcg tatgcagaac cacgtcctgt
     1021 ccaccgcctt caaagcctta atccgcgccc ttcccgccga caccgccgaa gtcgcgctcg
     1081 agtccatcca gcttgctttt gaggacgcct tggcagaatt gagctatgag gacagcccgc
     1141 atacggattt gttccacgac gttacttatg cgtttttccg tgaaaaagaa cgttaatttt
     1201 atgttaaact gattttttag gctttttgat taccgaaagg aattttgatg aatatgaaaa
     1261 aatggattgc cgccgccctt gcctgttccg cactcgcgct gtctgcctgc ggcggtcagg
     1321 gcaaagatgc cgccgcgccc gccgcaaacc ccgacaaagt gtaccgcgtg gcttccaacg
     1381 ccgagtttgc cccctttgaa tctttagact cgaaaggcaa tgtcgaaggt ttcgatgtgg
     1441 atttgatgaa cgcgatggcg aaggcgggca attttaaaat cgaattcaaa caccagccgt
     1501 gggacagcct tttccccgcc ttgaacaacg gcgatgcgga cgttgtgatg tcgggcgtaa
     1561 ccattaccga cgaccgcaaa cagtctatgg acttcagcga cccgtatttt gaaatcaccc
     1621 aagtcgtcct cgttccgaaa ggcaaaaaaa tatcttcttc cgacgatttg aaaaacatga
     1681 acaaagtcgg cgtggtaacc ggctacacgg gcgatttctc cgtatccaaa ctcttgggca
     1741 acgacaatcc gaaaatcgcg cgctttgaaa acgttcccct gattatcaaa gaactggaaa
     1801 acggcggctt ggattccgtg gtcagcgaca gcgcggtcat cgccaattat gtgaaaaaca
     1861 atccgaccaa agggatggac ttcgttaccc tgcccgactt caccaccgaa cactacggca
     1921 tcgcggtacg caaaggcgac gaagcaaccg tcaaaatgct gaacgatgcg ttggaaaaag
     1981 tacgcgaaag cggcgaatac gacaagattt acgccaaata ttttgcaaaa gaagacgaac
     2041 aggccgcaaa ataagcccgc ccatccgaac acaatgccgt ctgaagccct ttcagacggc
     2101 attgttcatc aatcggccta caatgaactg cctgctgatt tctccctacc gcaaagcaac
     2161 aggcaaagat tacaaatatc aaaatccgag taaaacagta ttttattaaa acaaattgat
     2221 aatcaagaga ttagaattat gtattgtctt taccgtacaa acgctggcac tatttcaacc
     2281 tgataaaaaa cagccttcaa aaaggttgct taaaacagca gcagacactt accgccacaa
     2341 ccttgaaaag gaacacaatc atgaccgtca tcaagcaaga agactttatc caaagtatct
     2401 gcgatgcctt ccaattcatc agctattacc atcccaaaga ctacatcgac gcgctttata
     2461 aggcgtggca gaaggaagaa aaccccgccg ccaaagacgc gatgacgcag attttggtca
     2521 acagccgtat gtgtgccgaa aacaaccgcc ccatctgcca agacacaggt atcgcaaccg
     2581 tgtttttgaa agtcggtatg gatgtgcagt gggatgcgga tatgagcgtg gaagaaatgg
     2641 ttaacgaagg cgtgcgccgc gcctacactt gggaaggcaa tacgctgcgc gcttccgtcc
     2701 tcgccgatcc ggccggcaaa cgccaaaaca ccaaagacaa cacccccgcc gtcattcata
     2761 tgagcatcgt gccgggcgac aaagtcgaag taacctgcgc agcaaaaggc ggcggctctg
     2821 aaaacaaatc caaactcgcc atgctcaatc cttccgacaa catcgtcgat tgggtattga
     2881 aaaccatccc gacgatgggc gcgggctggt gtcctcccgg catcttgggc atcggcatcg
     2941 gcggcacgcc tgaaaaagcc gtgttgatgg caaaagaatc cctgatgagc cacatcgaca
     3001 ttcaagagtt gcaggaaaag gccgcgtccg gcgcagaatt gtccaccacc gaagccctgc
     3061 gcctcgaact ctttgaaaaa gtcaacgcgc tgggcatcgg cgcacaaggt ttgggcggtc
     3121 tgaccaccgt gttggacgtg aaaatcctcg attacccgac ccacgccgcc tccaaaccga
     3181 ttgccatgat tccgaactgc gccgccaccc gccacgtcga atttgaattg gacggctcag
     3241 gccctgtcga actcacgccg ccgcgcgtcg aagactggcc cgatttgact tacagccccg
     3301 acaacggcaa acgcgtcgat gtcgataagc tgaccaaaga agaagtggca agctggaaaa
     3361 ccggcgacgt attgctgctg aacggtaaaa tcctcaccgg ccgcgatgcc gcacacaaac
     3421 gcctcgtcga tatgctcaac aaaggcgaag aattgcccgt cgatttcacc aaccgcctga
     3481 tttactacgt cggtcccgtc gatccggtcg gcgatgaagt cgtcggtccg gcaggtccga
     3541 ccacagccac ccgcatggac aaattcaccc gccaaatgct cgaacaaacc ggcctcttgg
     3601 gcatgatcgg caaatccgag cgcggcgtgg ccacctgcga agccatcgcc gacaacaaag
     3661 ccgtgtacct catggcagtc ggcggcgcgg cgtatctcgt ggcaaaagcc atcaaatctt
     3721 ccaaagtctt ggcgttcccc gaattgggca tggaagccat ttacgaattt gaagtcaaag
     3781 acatgcccgt aaccgtcgcc gtagatagca aaggcgaatc catccacgcc accgccccgc
     3841 gcaaatggca ggcgaaaatc ggcatcatcc ccgtcgaatc ttgaggcgcc atgccgtctg
     3901 aacacaaaat ctgccttcag acggcatttc cgcccccggt tgcggtacaa tccaccattt
     3961 catcactcgg cgacccacac cgtgaaaatc ctcattttag gcaacggaca ggtaggttct
     4021 accgtcgcac aaaaccttgc cgccataccc aacaacgacg taaccgttat cgacatcgac
     4081 gaaaaagcat tgcaggaaac aggcagccgc ctcgatgtcc aaaccgtttt cggcaacggc
     4141 gcatccccct tcacattaga acgcgccggc gcggaagatg ccgacttgct gctcgcgctc
     4201 tcccgcagcg acgaaaccaa catcgtcgcc tgcaaagttg ccgccgacct gttcaacatc
     4261 cccggccgca tcgcgcgcgt ccgttccagc gaatacctcg aatacctcag ccccaagctc
     4321 gaaaacaacg aaaacggcag cctttccata ttcggcataa ccgaaaccat cagccccgaa
     4381 cagctcgtta ccgaacagct tgccggcctg atagactgcc cgggcgcatt gcaggtttta
     4441 cgttttgcag acgaccgcgt gcggatggtc atcatacagg cgcggcgcgg cggactgctt
     4501 gtcggacgca gcattgccga catcgcccaa gatttgcccg acggggccga ctgccaaatc
     4561 tgcgccgttt accgcaacaa ccgcctcatc gtccccgcgc cgcaaaccgt catcatcgaa
     4621 ggcgacgaaa tcctatttgc cgccgccgcc gaaaacatcg gcgcggtcat acccgaattg
     4681 cgccccaaag aaaccagcac ccgccgcatc atgattgccg gcggcggcaa catcggctac
     4741 cgtctcgcca agcagctcga acacgcatac aacgtcaaaa tcatcgaatg ccggccgcgc
     4801 cgtgccgaat ggatagccga aaacctcgac aacaccctcg tcctgcaagg ttcggcaacc
     4861 gacgaaaccc tgctcgacaa cgaatacatc gacgaaatcg acgtattctg cgccctgacc
     4921 aacgacgacg aaagcaacat tatgtccgcc cttttggcga aaaacctcgg cgcgaagcgc
     4981 gtcatcggca tcgtcaaccg ctcaagctac gtcgatttgc tcgaaggcaa caaaatcgac
     5041 atcgtcgtct ccccccacct catcaccatc ggctcgatac tcgcccacat ccggcgcggc
     5101 gacatcgttg ccgtccaccc catccggcgc ggcacggcgg aagccatcga agtcgtcgca
     5161 cacggcgaca aaaaaacttc cgccatcatc ggcaggcgca tcagcggcat caaatggccc
     5221 gaaggctgcc acattgccgc cgtcgtccgc gccggaaccg gcgaaaccat tatgggacac
     5281 cataccgaaa ccgtcatcca agacggcgac cacatcatct ttttcgtctc gcgccggcgc
     5341 atcctgaacg aactggaaaa actcatccag gtcaaaatgg gctttttcgg ataaaccgcc
     5401 ccattccgga catattgccg ccaagcggta tggaagcgga ataatggtag gtgggcttca
     5461 gacggcatcc gccctccccg tcattcccgc gtaagcggga atccagacct tgggatagcg
     5521 gcaatattca aaggttataa aagacccgtc attcccgcgc aggcgggaat ccagaccttg
     5581 ggatagcggc aatattcaaa ggttagctga agctttagag attctagatt cccgtttcca
     5641 cgggaatgac gaaaggttgc gggaatccaa accattgggc aacagcaata ttcaaagatt
     5701 atctgaaagt ttgaggttct agattcccgc tttcgcggga atgacgaaaa gtggcgggaa
     5761 tgacgaagtt tcagacggca tcgcccgcct gttttgatat ggcggcaccc ccccgacaaa
     5821 aaaaacaatc cggaacgcat ctgaccgttc cggcttgttt tcaggcgaat ccgccgcatc
     5881 agaacatact gcgcacgccc atattgacct gccaagtctg gcgcatcgtg tgcatcgaag
     5941 acctttgcgc ctcaaaataa agctgccttc cgttgtcggc attaccacgc aaaaaaaatg
     6001 aattgcttga tattccaatg ttttttatat gtttttatat tgtgatgcga tcagacaaac
     6061 gcccccctga catttgttta gacggcatcg tattgctaaa tttctataag tatgtataat
     6121 gtccgtttcc acgcgcccat cgtctagagg cctaggacac tgccctttca cggcggcaac
     6181 cggggttcga atccccgtgg gcgtgccaat tcaaaaacct gcttgtttca agcaggtttt
     6241 tttattatga atcgtcattc ccgcac
//
LOCUS       DAWGJV010000120         6240 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWGJV010000120 DAWGJV010000000
VERSION     DAWGJV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.120"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(938..1075)
                     /locus_tag="WHQ94_001649"
     CDS             complement(938..1075)
                     /locus_tag="WHQ94_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128900.1"
                     /translation="MPSERLQTAFFTQSPPFSIILDNTVILKPVISAQAGIQSVRFRF
                     F"
     gene            complement(1093..3837)
                     /gene="tbpA"
                     /locus_tag="WHQ94_001650"
     CDS             complement(1093..3837)
                     /gene="tbpA"
                     /locus_tag="WHQ94_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ2128901.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYHQNAKRAYSSNLPTQNGNQKISPNGSTSTPYWVSIGRGNVVTEQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLGK
                     IDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
     gene            complement(3924..6065)
                     /locus_tag="WHQ94_001651"
     CDS             complement(3924..6065)
                     /locus_tag="WHQ94_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ2128902.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRFKRRNWHPQANPKEDEKKLSENDWEATGLPSDPKN
                     LPERQKSVIDKVETGSDSNIYSSPYLTQSNHQNGSINGGANLPKNEVTNYKDFKYVYS
                     GWFYKHAKQKIDFPNKIAQQGDDGYIFYHGKEPSRQLPASGTVTYKGVWHFATDTKKG
                     QKFREIIQPSKNQGDRYSGFSGDDGEEYSNKNEATLQGGQEGYGFTSNLEVDFNNKKL
                     TGKLIRNDKVTNATTSGKHTTQYYSLEAQVTGNRFNGKATATDKPGTGETKQHPFVSD
                     SSSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTQDKPGNGAAASDGAGAAASGDAA
                     DMPSENSKLTTVLDAVELTHSGKAIKNLDNFSNAAQLVVDGIMIPLLPKDSESGNNQA
                     NQGTNGGTAFTYKTTYTPKSDEKDTQAGTAENGNPAASNTAGDANGKTKTYEVEVCCS
                     NLNYLKYGLLTRKTAGNTGEGGNGSQTAAAQTAQGAQSMFLQGERTDEKEIPSEQNVV
                     YRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFGEKKINGTLTAENRQAATFTIEGT
                     IQDNGFSGTAKTADSGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGGWFAYPGD
                     KQAQPSASGSGASAANSATVVFGAKRQQLVQ"
BASE COUNT         1318 a         1595 c         1569 g         1758 t
ORIGIN      
        1 gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa aaaccttccg
       61 ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt aaataaattc
      121 ttgcagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc gaaagcggta
      181 atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat cccgtcattc
      241 ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc attcccacgc
      301 agtcgtgaat ccgaacgcgt ccgcacggaa acctgcatcc cgtcattccc acgaaagtgg
      361 gaatctagga cgtaaaatct caagaaaccg ttttacccga taagtttccg caccgacaga
      421 cctggattcc cgcctgcgcg ggaatgacga aatttcagcg agccgtaggg tgggctgtag
      481 ggtgggcttc agcccaccaa tttcaccgta tcaaaggttt ttgggaatac ggcggtttcg
      541 gttttcggtg ggaatgggcg gaatggataa cattgacgga gttgggggaa taggtggaaa
      601 cggtgggatt ggtgggctga agcccaccct acagccagcc ctatgtccac gctgcacatc
      661 ctacgcccgc cttgcgtttc ttataagcag cattttacgc aatactttgc gtccgctgaa
      721 aaaatttcaa actgtctttt ttatcaaatg cgaaaaatat aacgtcattc ccgcgcaggt
      781 gggaatctag tctgtcggtg cggaaactta tcggataaaa cggtttcttt agattttacg
      841 ccctagattc ccgcctgcgc gggaatgacg ggatttgagg tttctgtttt tgattttctg
      901 ttttcgtagg aatgacggtt tagagttacc cgaaacctca aaaaaaccga aaccgaacgg
      961 actggattcc cgcctgcgcg gaaatgacgg gtttcaagat tacggtattg tcgaggatga
     1021 tggaaaacgg tggggattgt gtaaaaaatg ccgtctgaag cctttcagac ggcatttgcg
     1081 gcgtttggac gtttagaact tcatttccaa gctaaatgtg tagttgcggc cgggggcggc
     1141 atatcggttg taaacgccga catttttgtg ttggttgact gcgccgccgg cagtttgccg
     1201 cacattttcc caagtaacat agcggtagtt gaggaggttg tacacgcccg cacggagggt
     1261 aaagtgtttt ttaatcgtgt aataaccgga cacatccaca atataccaag ggcgggtacg
     1321 gcgcgcggtg gcttttgtat tgcggctgtt gccgttgagc aaagcccggc tgcccaacaa
     1381 ctctgtgatt tccttggctt tggaataagt cagcataccg ttcacacccc atttgccttc
     1441 cggttggtca tagcccaagc cgacgacata gcgcgagggt tggatggcat caaacaggtg
     1501 tgattgaata tcggtgcggt ctgcgcgttt tttgatgtcg cggacatgga cacgattata
     1561 ggcaaatgta gaataccaac cttcgggcaa tttatcccat acgccgttcc aatcgatttt
     1621 gcccaaaaca ttgatgccgg taatccgcgc gctttgggca ttgaggtaag ccgggtcgcc
     1681 tttggctgtt tctttgccgt tttgaatttg cgcttcataa ccccggacaa tcaaatcgcg
     1741 gtaggcattg ttgaaccaac ttgcctccaa gttgccgaaa tcgcctttaa acacgatgcc
     1801 ggcttctttg ttgaacgatt tttccggatc gattttgacc gccttgcttt gaacacccgc
     1861 ccgccagccg tacatttccg caaacgaggg caggcggaag ccagttgaag tgcggtaagt
     1921 caaatccagc cagtcggcag gtttgaggac gatgcctgcg ttccaggaca gggtgcggtg
     1981 cgtaccggtg gaaacgctgc cgtcgtccga atgcgtgctg cggtagtcgt agcgcaaccc
     2041 cgcgccgaca tccgcccacc tgcccaaacg gacattgtcc cgaactgccg cgtaatagct
     2101 tttgccgttg atgctgcgcg gcgtgcagtc cgtataagta ttgttgccca agcggcagat
     2161 ttgctccgta accacatttc ccctgcctat gctgacccaa tagggagtgc ttgtgctgcc
     2221 gttggggctg attttttgat tgccgttttg agtgggcaga ttcgacgaat aggcacgttt
     2281 ggcgttttga tgataataat cctgatggcg gagattagag ccaaagcggt caaacccgag
     2341 attcacgctc aggttgtggc ggattttggc ggtatcgaag gattttttga atgccgcctg
     2401 caagagcctg tggctttccc cgtaaatcac gcgatcggat ttgtaatagg aaaacggctt
     2461 gtcggcactc ggacggcaat atttgtccga accgtcggca gaacagtgcg tctgctgaaa
     2521 atgattgtcc aaaccgatgc cctgacggtc gtaagagagg cgggcataat ccgcccaagt
     2581 gtctttatcg gcattggtat agacatattc caaaccgtag cggcttttgg tgtgcgtctc
     2641 gtcgtaaaac acgcccgtac cgtattccgc gcccaccagc gcaccgtttt cgccgttggt
     2701 aaacagtccg ccgtatttgt ggttgcccgc gtatttgccg ttgcctctta tcggtccgta
     2761 ttttctattt tcatcaaaaa ccgccttggt caggaatgcc ggaaccgtca tatcgcgcgt
     2821 gtcgaaagtt tgttgcgtgt gttcgagtat gccgccgatg tagtgccgct tattctcaaa
     2881 acgaaaaccc gggcggaaca gccacgaccg gctttcgtat gaaagcggat cggcgaggaa
     2941 gcggttggga cccgtgtagt ctcgggtgga aaccgtttga cgttcgtctt tgccgacaac
     3001 atcttttttc ggattcgctt tacacgtttc ataactcccg tttttgcatt cttctttaac
     3061 gatgaaatag gcgtaattgc tgctgtcttc aaccggcacc agcctgttaa agctctgaac
     3121 gccgcgtccg gcggcttcgt gggcgcggat ttcccccgcg cgccgacccg tgtggatcag
     3181 caaagcctcc gcaccgccga tgcgccccgc cagcgcgatg gattgggtaa gcccccggtt
     3241 tttgccggaa taggcggttt tactctgaat gccccactgc ctgccttccc cgataacatc
     3301 gtcggcggtt ttggtttgaa atgcgaccga acccgccaat gcgccgctgc cttgttcgac
     3361 cgagtttgag cctttgctga tttcgacagc cttgacgttt tcatactcga tttcattgat
     3421 tgcgccgctg ctgcccgccg tcctcgtccc gcccaatgcc gcctgcgcgg tgtaggactg
     3481 tatttgcgcc aagccgtcca ccgtcaagga aacgcggttt ttgtccatac cgcgtatcga
     3541 atagcccgag cttgcgccgc gcccctgttc gaccacggcg atgccggggt cgtaacgcgt
     3601 caggtcgcgg atatcgagta cctgttcctt gctgagggtg tcggcggttt tgaccaattt
     3661 gcccaaaccg gttacttcgt tatcccggcg ggttttctgt tttttggctt ttacctgtat
     3721 ggtatccaac tgtttttcct gtgcttgtcc ggcttgcaca ttttctgcat aaacgggcag
     3781 cgcagtcatt aaagacaggc ataaaatatt taatcggaac aaatgttgct gttgcatagt
     3841 gtttccctac tcttcgcttt cagacggcat cggaaggagc gatgccgtct gaagccttat
     3901 tctcgattgt tcggcagccg tgcttattgc acaagctgtt ggcgtttcgc accgaatacc
     3961 acggtcgcgc tgttggcggc tgatgcgcct gaccccgaag cggacggctg cgcctgcttg
     4021 tcgcccggat aggcaaacca tccgcccaac tcttcggctt tgggcccgta aaaaccgccc
     4081 tgcaccttgg cgtttgtgat atatgcctta ggcgtaccgg tggtattgct ctgatcgaga
     4141 tcaaaacctg agtcagcagt tttcgccgta ccggaaaaac cgttgtcctg aatcgttccc
     4201 tcaatggtaa aggttgccgc ctgcctgttt tcagcggtta acgttccgtt aatttttttc
     4261 tcgccaaaat tcacggtaaa ttccgccctg ttgccgccct ctttatctga agcattgccg
     4321 ctccagcttg tgccgttggc aatatgcccg taccaagacc cccgataaac gacgttttgc
     4381 tcgcttggaa tctctttttc atcggtgcgc tcgccttgga ggaacatact ttgtgcaccc
     4441 tgtgccgttt gggcggcggc ggtttggctg ccgttgccgc cttcccccgt gttgccggcg
     4501 gttttgcgtg tcagcaaccc gtatttcaaa taattgaggt tggaacagca gacttcgact
     4561 tcataggttt ttgttttgcc attggcatca cctgccgtat ttgaagcggc tggattgcca
     4621 ttctccgccg tacctgcttg ggtgtctttt tcatcgcttt tcggcgtgta ggttgttttg
     4681 taggtaaagg ctgttccgcc atttgtacct tgattggctt gattgttccc gctttcggaa
     4741 tccttgggca ggagcggaat cataatgccg tcgacaacca gttgggcggc gttgctgaag
     4801 ttgtcgagat ttttgattgc tttgccgctg tgcgtcagct cgaccgcatc caaaaccgtg
     4861 gtcagcttac tgttttcaga cggcatatct gccgcatcgc ccgatgctgc cgcacctgcg
     4921 ccgtctgaag ccgctgcgcc atttcccggt ttgtcttggg ttttcgcgct gccgacaacc
     4981 gcaacttttt tatcgtcgct caaaaagcgg aaacccaatt cctcaccctt cgggccgaaa
     5041 aagccgccgc tcaaagaaga cgagtcggaa acaaagggat gttgtttggt ttctccagtg
     5101 ccaggtttat cggttgccgt tgccttgccg ttgaagcggt tgcctgttac ttgagcctca
     5161 aggctgtaat attgcgtggt atgtttgccg ctagtagtag cgtttgtaac tttatcattg
     5221 cgtatcaatt tacccgtcaa tttcttatta ttgaaatcca cttctaaatt tgaggtaaaa
     5281 ccataaccct cttgaccgcc ttgtaaagtc gcttcatttt tattggaata ttcttcgccg
     5341 tcatcacccg aaaatccgct atacctgtcg ccttgatttt ttgaaggttg gataatttca
     5401 cgaaattttt gacccttttt tgtatcggtc gcaaaatgcc acacaccttt ataggtaact
     5461 gttccagaag cgggaagttg tcgggaaggt tctttgccgt gataaaagat atagccgtcg
     5521 tcgccttgtt gtgcaatttt atttggaaaa tcaatttttt gtttagcatg tttgtaaaac
     5581 cagccggaat aaacatattt gaaatcttta taatttgtta cttcgttttt tggcaggttt
     5641 gcaccgccat taatgctgcc attttgatgg tttgattgcg tgagataggg ggaagaataa
     5701 atattgctgt cgctgcctgt ttctacttta tcaataactg atttctgtcg ctcgggtaag
     5761 tttttagggt cacttggtaa tcccgtcgcc tcccaatcat tttcagaaag ttttttctca
     5821 tcttctttag gatttgcctg cggatgccaa ttccgccgct tgaagcgcat tgcaaaaccg
     5881 tatccgcctt ggtctttttg ggcttgcggt ttttcggaag aaacatcttg atattttggc
     5941 gcgggacgcg gggcttcggt atcgacagaa tcaagatcga aactgccgcc gcctccgccc
     6001 agacaagcac tcaacaaaaa caccggcagc accatagcag cctgatttac caatggattg
     6061 ttcatataaa cccaattcaa ttaaagaatg ataaggatta ttattttatt tatttttaac
     6121 aaatttgcaa atgctttttt atttttttag ggaatacacc aaaatcccgt cattcccgcg
     6181 cagtcgtgaa tccgaacgcg tccgcacgga aacctatatc ccgtcattcc cacgtatatc
//
LOCUS       DAWGJV010000121         6104 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWGJV010000121 DAWGJV010000000
VERSION     DAWGJV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.121"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(112..2502)
                     /gene="gyrB"
                     /locus_tag="WHQ94_001652"
     CDS             complement(112..2502)
                     /gene="gyrB"
                     /locus_tag="WHQ94_001652"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ2128903.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            2772..3644
                     /locus_tag="WHQ94_001653"
     CDS             2772..3644
                     /locus_tag="WHQ94_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ2128904.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3732..5768
                     /locus_tag="WHQ94_001654"
     CDS             3732..5768
                     /locus_tag="WHQ94_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ2128905.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
BASE COUNT         1348 a         1745 c         1619 g         1392 t
ORIGIN      
        1 aaatgctgat catggttttg ctccgaaagg cggtttcaga cggcatggct tccgtttgac
       61 aaaccaaaag gagacgcggc acgatgcccg tctccttttt taaaacggca cttatgcgtc
      121 gatattttgg gcaatcagcg cgttgttttc gataaaggcg cggcgcggtt cgacctcgtc
      181 gcccatcagg gtaacgaata cttcgtcggc ggcaatggca tcttcgatgc gcactttcaa
      241 caggcggcgc acggcgggat ccatcgtggt ttcccacagc tgttcggggt tcatctcgcc
      301 caagcctttg tatcgttgga tggacatacc tttttgggca acgctcatca agatgtccaa
      361 agcggtttca aagctgtccg cgtcgtaccc gttttcgcct ttgtaaagct tggcaccctc
      421 gccgaccatg cctttgagcg cggcggcggt ttgggtgagg gtttggtagg ctttgctgtt
      481 gaggaacttg ggttcgatgt agctgaccat gacgttgccg tgcagcttgc gcgtgatttt
      541 gatgaaccgg tgtccttcat gaccttcgat gcgttcgagg gcgacttctt tttcgtcaag
      601 cagaccggaa agttcggcaa cggctttatc ggcgttttca gacgacgtca aatcaatggg
      661 cgacgcgtgt agcatggcgc gcaggacgag ttcgtctacg aagcggcttt cctgttcgat
      721 gacggttttg gccaacagga attgtttggc ggtattggca agctcttcgc cttcgatggt
      781 gcggctgtct gaaacgattt tggctttttc caaggctaaa ccgagcagcc attggtcttt
      841 ttccaattcg tctttgagat atcgttcttg tttgccgtat ttggctttgt ataaaggcgg
      901 ctgggcaatg tagatgtagc cgcgttcgac cagctcgggc atttggcggt agaagaaggt
      961 taaaagcagg gtgcggatgt gcgcgccgtc cacatcggca tcggtcataa tgatgatgcg
     1021 gtggtaacgc agcttctcgg cattgaattc ttccttgccg atacctgcac ccaacgcagt
     1081 aatcaaagcg gcgacttcct gactggcgag cattttttca aagcgcgcct tttccacgtt
     1141 caaaatcttg cccttaagcg gcaaaatcgc ttggaatttg cggtcgcgcc cctgcatggc
     1201 ggaaccgcct gcagaatcgc cctcgaccag ataaagttct gacaatgcgg ggtctttttc
     1261 ttggcagtcg gcgagtttgc cgggtagccc caagccgtcc atcacgcctt tgcggcgggt
     1321 gatttcgcgt gctttgcggg cggcttcgcg cgcgcgggcg gcatcgacga ttttgccggt
     1381 gatgattttg gcttcggcag ggttttcttc gaggaagtcg gtcagggctt ggctgatgac
     1441 ttcgttgacg acgggaccga tttcgccgga aaccagtttg tctttggttt gggacgaaaa
     1501 tttggggtcg ggcagtttga cggacaacac gcaggtcaaa ccttcacgca tatcgtcgcc
     1561 ggcggtttcc actttggctt ttttggcgac ttcgttggct tcgatgtagc tgttgatggt
     1621 gcgcgtcatc acttggcgca acgcggtcag gtgcgtaccg ccgtcgcgct gagggatgtt
     1681 gttggtgaag cactgcacgc tttcctgata gctgtcgttc cattgcattg cgcattcgac
     1741 gctcatgccg tctttctcgc cgaacgcgta gaagattttt tcgtgcaagg gcgttttttt
     1801 gcggttcatg tattgcacga atccagccac gccgccggaa agggcgaagc tttcgtgttt
     1861 gccgtcgcgc tcgtcggtca attcgatgtc cacgccgttg ttcaggaacg aaagttcgcg
     1921 gatgcgtttg gcgaggatgt cgaagctgta ttcgacgttg ccgaaggttt ccgcactggc
     1981 gaggaagcgc acggtcgtgc cttttttatc ggaatcgccg acaacgttca gcggctcttc
     2041 ggtttcgccg cgcacgaagc ggacgaagtg ttctttgccg tcgcggtaga tggtcagcgt
     2101 tacccagtcg gacagcgcgt tgacgacgga cacgcccacg ccgtgcaggc cgccggagat
     2161 tttgtagctg ttgttgtcga atttaccgcc cgcgtgcaat acggtcatga tgacttcggc
     2221 ggcggagcgt ccttctttcg ggtggatgcc ggtgggcata ccgcgcccgt tgtcggcgac
     2281 gctgacggaa tggtcggcgt gtatcgttac cgtgattttg tcgcaatgtc cggcgagtgc
     2341 ttcgtcaatg gcgttgtcca atacttcaaa caccatatgg tgcagaccgc tgccgtcctg
     2401 cgtgtcgccg atgtacatgc cggggcgttt gcgtaccgct tccaagcctt cgagcacctg
     2461 aatgctgtcg gcgccgtatt cttcgtgttt ttgttcagtc atattttttg ccggattttg
     2521 aaaagatttt gcgatgccgc caaaacaagt ccgcaccttg tggaaaaagc gggcgggacg
     2581 gcatatataa ttgtgtatta tagccgattt tgccgcctaa ttcagcgtta tccgcatcag
     2641 tgtgccgccg ggaaaagatg aaacggtacg tttgcctccg gcatcaggtc ggggattttc
     2701 ccgtaaagtg gcaaaagcgt ttttttgcca ctaaaatcta caccctatac ttttcggaca
     2761 ggggcgcgga aatggaaata tggaatatgt tggacacttg gctcggtgcc gtcccgatac
     2821 gtgcggaggc ggtcgaatcc gtggcggcgg ttgcggcttt gctgctggcg cgcgcccttc
     2881 tgttgaatat ccacttcaaa cggcatccgg atttcggcat cgaaagcaag cggcggtttt
     2941 tggttgccag ccgcaatata acgctgcttt tggtgctgtt ttcgctggca tttatctggt
     3001 cggcgcaaat ccaaacgctg gctttgtcga tgtttgcggt ggcggcggcg gtcgtcgtgg
     3061 cgacgaagga actgattatg tgtctgtcgg gcagtatttt aaggtctgcc acccagcaat
     3121 actcggtcgg cgactatatc gaaatcaacg gcctgcgcgg gcgcgtggtc gacatcaacc
     3181 tgttgaacac gctgatgatg caggtcggtc cgaacccctt ggtcggacag cttgcgggaa
     3241 ccaccgtttc tttccccaac agcctgttgt tgagccaccc cgtgcgccgc gacaatattt
     3301 tgggcgacta tgtcatccat acggtcgaaa tccccgttcc catccatttg gattcggatg
     3361 aagccgtatg ccgtctgaaa gccgtactcg agcccttgtg cgcgccctac atccccgcca
     3421 tccaacggca tttggaaaac gtgcaggcgg aaaaactgtt tatcacgccc gccgccagac
     3481 cgcgcgttac ccgcgtgccg tacgatgaca aggcataccg catcatcgtc cgcttcgcct
     3541 cccccgtttc aaagcggctg gaaatccaac aggcggttat ggacgaattt ttgcgcgtac
     3601 aataccgcct gttaaattac cccgccggct ccgaaacact ttaactttcc ctgaccgacc
     3661 ccatttccgg cttcagacgg catattgccg atatgctgtc tgaagcacaa cacgcaaagg
     3721 aaacccatct tatgactgac aacgcactgc tccatttggg cgaagaaccc cgttttgatc
     3781 aaatcaaaac cgaagacatc aaacccgccc tgcaaaccgc cattgccgaa gcgcgcgaac
     3841 aaatcgccgc catcaaagcc caaacgcaca ccggctgggc aaacactgtc gaacccctga
     3901 ccggcatcac cgaacgcgtc ggcaggattt ggggcgtggt gtcgcacctc aactccgtcg
     3961 ccgacacgcc cgaactgcgc gccgtctata acgaactgat gcccgaaatc accgtcttct
     4021 tcaccgaaat cggacaagac atcgagctgt acaaccgctt caaaaccatc aaaaactccc
     4081 ccgagttcga caccctctcc cccgcgcaaa aaaccaaact caaccacgat ctgcgcgatt
     4141 tcgtcctcag cggcgcggaa ctgccgcccg aacagcaggc agaactggca aaactgcaaa
     4201 ccgaaggcgc gcaactttcc gccaaattct cccaaaacgt cctagacgcg accgacgcgt
     4261 tcggcattta ctttgacgat gccgcaccgc ttgccggcat tcccgaagac gcgctcgcca
     4321 tgtttgccgc cgccgcgcaa agcgaaagca aaacaggcta caaaatcggc ttgcagattc
     4381 cacactacct cgccgtcatc caatacgccg acaaccgcga actgcgcgaa caaatctacc
     4441 gcgcctacgt tacccgcgcc agcgaacttt cagacgacgg caaattcgac aacaccgcca
     4501 acatcgaccg cacgctcgca aacgccctgc aaaccgccaa actgctcggc ttcaaaaact
     4561 acgccgaatt gtcgctggca accaaaatgg cggacacgcc cgaacaagtt ttaaacttcc
     4621 tgcacgacct cgcccgccgc gccaaaccct acgccgaaaa agacctcgcc gaagtcaaag
     4681 ccttcgcccg cgaaagcctg aacctcgccg atttgcaacc gtgggacttg ggctacgcca
     4741 gcgaaaaact gcgcgaagcc aaatacgcgt tcagcgaaac cgaagtcaaa aaatacttcc
     4801 ccgtcggcaa agtattaaac ggactgttcg cccaaatcaa aaaactctac ggcatcggat
     4861 ttaccgaaaa aaccgtcccc gtctggcaca aagacgtgcg ctattttgaa ttgcaacaaa
     4921 acggcgaaac cataggcggc gtttatatgg atttgtacgc acgcgaaggc aaacgcggcg
     4981 gcgcgtggat gaacgactac aaaggtcgcc gccgtttttc agacggcacg ctgcaactgc
     5041 ccaccgccta cctcgtctgc aacttcgccc cgcccgtcgg cggcagagaa gcgcgtttga
     5101 gccacgacga aatcctcatc ctcttccacg aaaccggaca cgggctgcac cacctgctta
     5161 cccaagtgga cgaactgggc gtgtccggta tcaacggcgt agaatgggac gcggtcgaac
     5221 tgcccagcca gtttatggaa aatttcgttt gggaatacaa tgtattggcg caaatgtcca
     5281 cccacgaaga aaccggcgag cccctgccga aagaactctt cgacaaaatg ctcgccgcca
     5341 aaaacttcca acgcggaatg ttcctcgtcc gccagatgga gttcgccctc ttcgatatga
     5401 tgatttacag cgaagacgac gaaggccgtc tgaaaaactg gcaacaggtt ttagacagcg
     5461 tgcgcaaaga agtcgccgtc atccaaccgc ccgaatacaa ccgcttcgcc ttgagcttcg
     5521 gccacatctt cgcaggcggc tattccgcag gctattacag ctacgcgtgg gcggaagtat
     5581 tgagcgcgga cgcatacgcc gcctttgaag aaagcgacga tgtcgccgcc acaggcaaac
     5641 gcttttggca ggaaatcctc gccgtcggcg gatcgcgcag cgcggcagaa tccttcaaag
     5701 ccttccgcgg ccgcgaaccg agcatagacg cactcttgcg ccacagcggt ttcgacaacg
     5761 cggcctgatc gatgtgaaca aaaaatgccg tctgaaacaa tcaaacagtg tttcagacgg
     5821 catcagcatt tgcacggttt cgccattttc acaaaaacag taagccaaaa tcccgtcatt
     5881 cccgcgcagg cgggaatcca gaccggtcgg cacggaaact tatcgggtaa aaaggtttct
     5941 ccggtcctga atcctagatt cccgctttcg cgggaatgac gcggaaaagt tgctgtgatt
     6001 tcggataaat tttcgcaacg tttaatttcc gttttacctg ataaatgccc gcaatctcaa
     6061 atcccgtcat tccccaaaaa caaaaaaaat caaaaacaga aatc
//
LOCUS       DAWGJV010000122         6023 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWGJV010000122 DAWGJV010000000
VERSION     DAWGJV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.122"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WHQ94_001655"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WHQ94_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ2128906.1"
                     /translation="MDTTLKTTLTSVAAAFALSSCTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WHQ94_001656"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WHQ94_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ2128907.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAYEHEMAIRHASEAGITGSNDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WHQ94_001657"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WHQ94_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ2128908.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1358 a         1702 c         1613 g         1350 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcggcgcgta tgtcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggaggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg taggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg ttcgaaccgg ttatgcccgc ttctgatgca
     1561 tgtctgatcg ccatttcatg ctcgtatgca gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccactccagg
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattgttg cggtcttgca ggttgatgct cagacccgaa
     2701 ccgttgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg cagacgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ctgcctgctg tggaacgatg aatgcattat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gccggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc agtttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagatttg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg ataatgccgc caacttgcgc acggacatcg gcggtacgca
     5761 gcgattccaa acgtcccggc aactcgacgg tcaacgcaac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gacccgcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAWGJV010000123         6003 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWGJV010000123 DAWGJV010000000
VERSION     DAWGJV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.123"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..421)
                     /locus_tag="WHQ94_001658"
     CDS             complement(<1..421)
                     /locus_tag="WHQ94_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ2128909.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPA"
     gene            complement(457..570)
                     /gene="rrf"
                     /locus_tag="WHQ94_001659"
     rRNA            complement(457..570)
                     /gene="rrf"
                     /locus_tag="WHQ94_001659"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(667..3558)
                     /locus_tag="WHQ94_001660"
     rRNA            complement(667..3558)
                     /locus_tag="WHQ94_001660"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3967..4042)
                     /locus_tag="WHQ94_001661"
     tRNA            complement(3967..4042)
                     /locus_tag="WHQ94_001661"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4007..4009),aa:Ala,seq:tgc)
     gene            complement(4048..4124)
                     /locus_tag="WHQ94_001662"
     tRNA            complement(4048..4124)
                     /locus_tag="WHQ94_001662"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4088..4090),aa:Ile,seq:gat)
     gene            complement(4226..5766)
                     /locus_tag="WHQ94_001663"
     rRNA            complement(4226..5766)
                     /locus_tag="WHQ94_001663"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1304 a         1756 c         1291 g         1652 t
ORIGIN      
        1 gtgcgggcgc accgacggaa gcctgatcct tcagcttcgc cagcaccgca gggccgatgc
       61 cctttacctt ggtcaaatcg tctacagact tgaacgcacc gttttgcgca cggtattccg
      121 caatggcctt cgccttcgcc gggcctatgc ctggcagcgc ctccaactcc tgcagcgaag
      181 ccgcattgat gtttaccgcc gcaagggaga aggcgcagga gaacagcata cagaacagca
      241 cgaacatttt cttcatggtt tttcctttaa gggttgcaaa caataaaccg catcttgcga
      301 cgataaaacg agtcattcta aaatgaatat cccaaagttt caagccgttc ctccgcaaac
      361 ccgaccggac gccgtacgga tgccgtcccg ccatccccga cattttttcc gggcaaagca
      421 aaaacccccg aataatcggg ggttttctga atgggtgttt ggcagtgacc tactttcgca
      481 tggaagaacc acactatcat cggcgctgag tcgtttcacg gtcctgttcg ggatgggaag
      541 gcgtgggacc aactcgctat ggccgccaaa cttaaactgt tacaaatcgg taaagcctta
      601 atcaatatat tcggtaatga ctgaatcagt cagtaagctt ttatctcttg aagttcttca
      661 aatgatagag tcaagcctca cgagcaatta gtatgggtta gcttcacgcg ttaccgcgct
      721 tccacacccc acctatcaac gtcctggtct cgaacgactc tttagtgcgg ttaaaccgca
      781 agggaagtct catcttcagg cgagtttcgc gcttagatgc tttcagcgct tatctcttcc
      841 gaacttagct acccggctat gcaactggcg ttacaaccgg tacaccagag gttcgtccac
      901 tccggtcctc tcgtactagg agcagccccc gtcaaacttc caacgcccac tgcagatagg
      961 gaccaaactg tctcacgacg ttttaaaccc agctcacgta ccactttaaa tggcgaacag
     1021 ccataccctt gggaccgact acagccccag gatgtgatga gccgacatcg aggtgccaaa
     1081 ctccgccgtc gatatgaact cttgggcgga atcagcctgt tatccccgga gtacctttta
     1141 tccgttgagc gatggccctt ccatacagaa ccaccggatc actatgtcct gctttcgcac
     1201 ctgctcgact tgtcggtctc gcagttaagc taccttttgc cattgcacta tcagtccgat
     1261 ttccgaccgg acctaggtaa ccttcgaact cctccgttac gctttgggag gagaccgccc
     1321 cagtcaaact gcctaccatg cacggtcccc gacccggatg acgggtctag gttagaacct
     1381 caaagacacc agggtggtat ttcaaggacg gctccacaga gactggcgtc tctgcttcaa
     1441 agcctcccac ctatcctaca caagtgactt caaagtccaa tgcaaagcta cagtaaaggt
     1501 tcacggggtc tttccgtcta gcagcgggta gattgcatct tcacaaccac ttcaacttcg
     1561 ctgagtctca ggaggagaca gtgtggccat cgttacgcca ttcgtgcggg tcggaactta
     1621 cccgacaagg aatttcgcta ccttaggacc gttatagtta cggccgccgt ttactggggc
     1681 ttcgatccga tgctctcaca tcttcaatta accttccagc accgggcagg cgtcacaccc
     1741 tatacgtcca ctttcgtgtt agcagagtgc tgtgttttta ataaacagtc gcagccacct
     1801 attctctgcg accctccggg gcttacggag caagtcctta accttagagg gcataccttc
     1861 tcccgaagtt acggtatcaa tttgccgagt tccttctcct gagttctctc aagcgcctta
     1921 gaattctcat cctgcccacc tgtgtcggtt tgcggtacgg ttcgattcaa actgaagctt
     1981 agtggctttt cctggaagcg tggtatcggt tacttcgtgt ccgtagacac tcgtcgtcac
     2041 ttctcggtgt taagaagacc cggatttgcc taagtcttcc acctaccggc ttaaacaagc
     2101 tattccaaca gcttgccaac ctaaccttct ccgtccccac atcgcatttg aatcaagtac
     2161 aggaatatta acctgtttcc catcgactac gcatttctgc ctcgccttag gggccgactc
     2221 accctacgcc gatgaacgtt gcgcaggaaa ccttgggctt tcggcgagcg ggcttttcac
     2281 ccgctttatc gctactcatg tcaacattcg cacttctgat acctccagca cactttacaa
     2341 tgcaccttca tcggcctaca gaacgctccc ctaccatgcc ggtaaaccgg catccgcagc
     2401 ttcggttata gatttgagcc ccgttacatc ttccgcgcag gacgactcga ccagtgagct
     2461 attacgcttt ctttaaatga tggctgcttc taagccaaca tcctggctgt ctgggccttc
     2521 ccacttcgtt taccacttaa tctatcattg gggaccttag ctggcggtct gggttgtttc
     2581 cctcttgaca acggacgtta gcacccgctg tctgtctccc gaggaaccac ttgatggtat
     2641 tctgagtttg ccatgggttg gtaagttgca ataaccccct agccataaca gtgctttacc
     2701 cccatcagtg tcttgctcga ggcactacct aaatagtttt cggggagaac cagctatctc
     2761 cgagtttgtt tagcctttca cccctatcca cagctcatcc ccgcattttg caacatgcgt
     2821 gggttcggtc ctccagtacc tgttacggca ccttcaacct ggccatggat agatcactcg
     2881 gtttcgggtc tacacccagc aactcatcgc cctattaaga ctcggtttcc ctacgcctcc
     2941 cctattcggt taagctcgct actgaatgta agtcgttgac ccattataca aaaggtacgc
     3001 agtcacacct ctagggtgct cccactgttt gtatgcatca ggtttcaggt tctgtttcac
     3061 tcccctcccg gggttctttt cgcctttccc tcacggtact ggttcactat cggtcgatga
     3121 tgagtattta gccttggagg atggtccccc catattcaga caggatttca cgtgtcccgc
     3181 cctacttttc gtacgcttag taccgctgtt gagatttcga atacgggact gtcacccact
     3241 atggtcaagc ttcccagctt gttcttctat ctcgacagtt attgcgtaca ggctcctccg
     3301 cgttcgctcg ccactacttg cggaatctcg gttgatttct tttcctccgg gtacttagat
     3361 ggttcagttc tccgggttcg cttctctaag tctatgtatt caacttagga tactgcacag
     3421 aatgcagtgg gtttccccat tcggacatcg cgggatcatt gctttattgc cagctccccc
     3481 gcgcttttcg caggcttaca cgtccttcgt cgcctatcat cgccaaggca tccacctgat
     3541 gcacttattc acttgactct atcatttcaa gaacttcttt gacttcgttt gcctacctgt
     3601 tgactaagta aaagcaaact tgaaattcct actttgataa agcttactgc tttgttgtgt
     3661 cttaatcctg ccttttgtgt ttcaggatta agtcgataca atcatcaccc aaatactgtg
     3721 tttgttttct tttctcttgt gagcgatttt ttatcctttg caaagaataa aaaatcaaaa
     3781 caaacgcttt gtctttgttt gttgatttcg gctttccaat ttgttaaaga tcgatgcgtt
     3841 cgatattgct atctactgtg caaatcaaaa cgagctgatt attatatcag cattttgttc
     3901 ttggtcaagt gtgatgtcgc cctgaatgga ttctgttcca ttcttccgtt ttgatttgta
     3961 cagtattggt ggaggcaaac gggatcgaac cgatgacccc ctgcttgcaa agcaggtgct
     4021 ctaccaactg agctatgccc ccgttattgg tgggtctggg aggacttgaa cctccgaccc
     4081 cacgcttatc aagcgtgtgc tctaaccagc tgagctacaa acccggattc tcttcttaag
     4141 cgaatcttgc cttcactcaa gcttcttccg catctttttc agtttaccga taagtgtgaa
     4201 tgcctaaagc ctcttctttc tctagaaagg aggtgatcca gccgcaggtt cccctacggc
     4261 taccttgtta cgacttcacc ccagtcatga agcataccgt ggtaagcgga ctccttgcgg
     4321 ttatcctacc tacttctggt atcccccact cccatggtgt gacgggcggt gtgtacaaga
     4381 cccgggaacg tattcaccgc agtatgctga cctgcgatta ctagcgattc cgacttcatg
     4441 cactcgagtt gcagagtgca atccggacta cgatcggttt tgtgagattg gctccacctc
     4501 gcggcttggc taccctctgt accgaccatt gtatgacgtg tgaagccctg gtcataaggg
     4561 ccatgaggac ttgacgtcat ccccaccttc ctccggcttg tcaccggcag tctcattaga
     4621 gtgcccaact gaatgatggc aactaatgac aagggttgcg ctcgttgcgg gacttaaccc
     4681 aacatctcac gacacgagct gacgacagcc atgcagcacc tgtgttacgg ctcccgaagg
     4741 cactcctccg tctctggagg attccgtaca tgtcaagacc aggtaaggtt cttcgcgttg
     4801 catcgaatta atccacatca tccaccgctt gtgcgggtcc ccgtcaattc ctttgagttt
     4861 taatcttgcg accgtactcc ccaggcggtc aatttcacgc gttagctacg ctaccaagca
     4921 atcaagttgc ccaacagcta attgacatcg tttagggcgt ggactaccag ggtatctaat
     4981 cctgtttgct acccacgctt tcgggcatga acgtcagtgt tatcccagga ggctgccttc
     5041 gccatcggta ttcctccaca tctctacgca tttcactgct acacgtggaa ttctacctcc
     5101 ctctgacaca ctcgagtcac ccagttcaga acgcagttcc cgggttgagc ccggggattt
     5161 cacatcctgc ttaagtaacc gtctgcgccc gctttacgcc cagtaattcc gattaacgct
     5221 cgcaccctac gtattaccgc ggctgctggc acgtagttag ccggtgctta ttcttcaggt
     5281 accgtcatca gccgctgata ttagcaacag ccttttcttc cctgacaaaa gtcctttaca
     5341 acccgaaggc cttcttcaga cacgcggcat ggctggatca ggcttgcgcc cattgtccaa
     5401 aattccccac tgctgcctcc cgtaggagtc tgggccgtgt ctcagtccca gtgtggcgga
     5461 tcatcctctc agacccgcta ctgatcgtcg ccttggtagg cctttacccc accaaccagc
     5521 taatcagata tcggccgctc gaatagcgca aggcccgaaa gtcccctgct ttctctctca
     5581 agacgtatgc ggtattagct gatctttcga tcagttatcc cccactactc ggtacgttcc
     5641 gatatgttac tcacccgttc gccactcgcc acccaagaag caagcttctc tgtgctgccg
     5701 tccgacttgc atgtgtaaag catgccgcca gcgttcaatc tgagccagga tcaaactctt
     5761 atgttcaatc tctaactttt taacttctgg tctgcttcaa agaaaccaac aggacaatgt
     5821 tcaaaacatt atcttgtctg tctttcaaac agtgtgaggc tcaaggcact cacacttatc
     5881 ggtaatctgt tttgttaaag agcgttgcga attataaagt attccttccg cctgtcaaga
     5941 tatctctcga tatccccaac attctgtgct atacttttca gttcgtccgc cgcttctgca
     6001 gca
//
LOCUS       DAWGJV010000124         5961 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWGJV010000124 DAWGJV010000000
VERSION     DAWGJV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.124"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(41..964)
                     /gene="truB"
                     /locus_tag="WHQ94_001664"
     CDS             complement(41..964)
                     /gene="truB"
                     /locus_tag="WHQ94_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ2128910.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="WHQ94_001665"
     CDS             complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="WHQ94_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ2128911.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAGKPVED"
     gene            1558..2802
                     /gene="clpX"
                     /locus_tag="WHQ94_001666"
     CDS             1558..2802
                     /gene="clpX"
                     /locus_tag="WHQ94_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ2128912.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTSSESVGQTSEEPGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADVTKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFEP"
     gene            complement(2905..4101)
                     /locus_tag="WHQ94_001667"
     CDS             complement(2905..4101)
                     /locus_tag="WHQ94_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ2128913.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHALTEFA
                     ATSDNP"
     gene            4505..5005
                     /locus_tag="WHQ94_001668"
     CDS             4505..5005
                     /locus_tag="WHQ94_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128914.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5090..5644
                     /locus_tag="WHQ94_001669"
     CDS             5090..5644
                     /locus_tag="WHQ94_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128915.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1407 a         1595 c         1619 g         1340 t
ORIGIN      
        1 aacacattat tcaaacacag cctgtatttt taaccgccgt tcaggcggcg gatgccgccg
       61 tgttcatcag gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccagaccga
      121 caaagcggcc gttttccgtg taaacgcgta ccggcgtgtc ggaaggcagg tcttcttcga
      181 aacgcggacg ttgtccgcag tggagcatat ggacggcata atcgtttaaa acggtttggg
      241 gaaagtgtga aaccaatacg tcgcagggta gcagcaagct gtcgcgttct gtttcgttca
      301 aatttgccaa ggcctcaagc gtgtggcttt gggcgatggt aaagccggcg gtttcagtgc
      361 ggcgcagggc ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg
      421 tgcggatgta ggtgcctttg ctgcaacgta cgtcgatgac ggctttgggc gcgtcaaatt
      481 cggcaatatc gatggcgtaa acggtaatgt cgcgcgcttt gcgttcgatg acgatgcctt
      541 tgcgggcgta ttcgtacagc ggtttgcctt cgtgcttgag cgcggaaaac attggcggca
      601 cttggcggat gttgcctgtc agtgcttggc aggccgtctg aaattcggct aaggaaatat
      661 cggcgcgggc ggtggcaatg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga
      721 gtttcagcgt ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg
      781 cttcaccgaa gcagacgggc aaaagtccgg ttgccaaagg gtcgagcacg ccggtatgtc
      841 cggctttttc ggcatgaaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc
      901 cttcgggttt gtccaagagc aaaacaccgt tgacggcacg tttttggggt ttgcctgtat
      961 tcatggttcg atgttcggac ggcatgggca tatccgtcag tcttcaaccg gttttcccgc
     1021 cgctacttgg tcgataaggg cggacaggtt caaaccgcgt tccaaagatt cgtcgtattt
     1081 gaaatgcagt tcgggcgttt tgaacagctt gatgcgtttg gccaattcgc tgcggaggtg
     1141 tccgcgcgcg tgttccagca cttcttccgt aatttcgcgc gcgtcttggt ttaaaacggt
     1201 gtagaacacg gtggcgtggc tgtaatcgcg ggtaacttcg acttcgttga cggtaatgaa
     1261 gccggcgcgc gggtctttca gtccggtacg gacgagttcg gcaagctcgc gcataatttg
     1321 ttctttgaca cggtcttggc gggcatagcc gcgttggggt tttctcataa atatcctcag
     1381 atttcagatt tggtttcaga cggcattttt gccgccgggg cggttgcgaa agggcgtatt
     1441 atagccgaat cggcgggatt ttaaggtaat acattgataa gaacggatat agttattata
     1501 cttggcaacc cgaacggcgc gtgccgtttc tgtaacgcat acattgtaaa gaaagctatg
     1561 tccaacgaaa accgtacctg ttccttttgc ggaaaatcca aatcacacgt caaacattta
     1621 attgagggcg aaaacgcctt tatctgtgac gaatgcgtct caaactgtat tgaaatattg
     1681 catgaagacg gaaatgacgg cacgtcttcg gaaagtgtcg gacagacatc ggaagagccc
     1741 ggcaagctgc ccacgcccgc cgaaatcgtc gccaacctca acgaccacgt tatcggtcag
     1801 gaacaagcca agaaagcctt ggcggtgtcg gtgtacaacc attacaaacg cctgcgccac
     1861 ccgaaagccg gagctaatgt cgaattgtcg aaatccaaca tcctgcttat cggcccgacc
     1921 ggatcgggta aaaccctgct ggcgcaatct ttggcgcgca aactggatgt gccgttcgtg
     1981 atggcagatg caaccacgct gaccgaagca ggttatgtcg gcgaagatgt cgagcaaatc
     2041 atcaccaaac tcttgggcaa atgtgatttc gatgtcgaaa aagcccaacg cggcatcgtc
     2101 tatatcgacg aaatcgataa gatttcacgc aaaagcgaca atccgtccat cacgcgcgac
     2161 gtgtccggcg aaggcgtgca gcaagccttg ctgaaactga ttgaaggtac ggtggccagc
     2221 gttccgcccc aaggcggccg caagcatccg aatcaggaat ttatcaacgt cgataccacc
     2281 aacatcctgt ttatctgcgg cggcgcgttt gcaggcttgg aaaaagtcat ccgccagcgt
     2341 actgaaaaag gcggtatcgg tttcggtgcg tccgttcaca gcaaggacga aaatgccgat
     2401 gttaccaagc tgttcggcat tgtcgaaccg gaagatttga ttaaattcgg tctgattccg
     2461 gaattaatcg gacgtttgcc cgtgattgca acgttagaag aactggatga ggacgcgctg
     2521 attaatattt tgaccgagcc gaaaaatgct ttggtcaagc agtatcaagc tttgttcggt
     2581 atggaaaatg tcgagttgga atttgaagaa ggcgcattgc gttccatcgc gcggcaggcg
     2641 atggaacgca aaaccggcgc gcgcggcctg cgttccatcg tcgaacgctg cctgctcgat
     2701 acgatgtacc gcctgcctga tttgaaaggc ttgaaaaaag tggttgttgg taaggcagtc
     2761 atcgaagagg gcagggaacc ggaattggtg ttcgagccct gatggcaaac aaaaccgctt
     2821 cggaacgtcg aaccacgttt ccgaagcggt tttttgtttt tgatgccgtc tgaagccttc
     2881 ccgccttcag acggcatttg agttttacgg attgtctgat gtcgcggcaa attccgtcag
     2941 cgcgtgttcc agacgccgca aaccttccgc cgtatcttca tcgtccaaca gcagcgaagg
     3001 cgcgaaacgc aatacgtccg cacctgcaac caaaatcatc acgccgtgtt tcaaggcggc
     3061 ggcggtgatt tcggatgcgc gtccgcgata aggcgcgtcc aacacgcagc cgagcagcag
     3121 tcccatcccg cgaacctgtg agaacaagcc tgttttcctg cccaaatcca gcaatgccgt
     3181 ctgaagtttc tgcccctggc tgcggacatg gtttaaagtt tcgggcgtat tgatgatgtc
     3241 gaatgcgcgg ctgccgaccg cacacgccat cgggttgccg ccgaaagtcg agccgtgcgt
     3301 gcccggttgg aaggcggcgg caatcttttc tgtcgccagc atcgcgccga tcggaaagcc
     3361 gcagcccaag gcttttgccg aactcaaaat atcgggaaca atgccgtaat gttcataggc
     3421 aaacagcctg cccgtatgcc ccatcccggt ttgaacttcg tccaaaatca acaacgcatt
     3481 gtgccggtcg cacagacggc gcgcggtttg caaatattcc gcagtggcgg gcaggatgcc
     3541 gctttcgccc tgtatcggct cgatgatgac cgcgcaggtc tgttcgccga cggcagcttc
     3601 cagcgcggca atatcgttga acggaacatg cgtaatgcct tgcggcaggg gtgcataatc
     3661 cttgctgtat ttcggctgac cgccgacgga cacggtaaac agcgtgcgtc cgtgaaaact
     3721 gttgatacag gcgacgattt cgctttttcc tccgccgaaa cggtcgcggg cgtatttcct
     3781 cgccagcttc aacgccgcct cattcgcttc cgagcccgag ttgcagaaaa aaaccttgtc
     3841 ggcaaagctg ttcttcacca atttttgcgc caactcctgc gccggccgtg tcgtatagat
     3901 attggaaatg tgccacagct tgtgcatctg cgtgtttaaa gcatcggcaa gggcagggtg
     3961 gcagtgtccc agcgcattga cggcgatgcc gcctgaaaag tcaatatatt cacgcccttc
     4021 cgtatcccaa acgcggctgc ctgaagcgcg ttcgggaatc atcggtgcaa aggcgaaatt
     4081 gggggtcaga tagttttgca taattgctcc tgattgcggt tttgctcggg attatgcacc
     4141 gtttttcaaa gcagataaag ggaaattgcc gacaatatcg ggcagcggta aaaataaacc
     4201 gaaatatttt caatcggata gatgaaaaat caaaaaaatt tcgataaaat gcttgaggcg
     4261 cggcagcgtt ttgatttaga atctccccaa tatttttatc ttcagacggc atttcatcat
     4321 gcaaggcaaa aaatttacaa ccgcttattg ctattggtac cacacagacg tgcggtactt
     4381 ttttgcgctt gcttgaaatc catcccatac gcaaaacagg ccgctcgaaa atggggcggc
     4441 ttttttgtta ccttcacaca ggtcgcccga ttcggcaaca cacgacagaa tcaggaaaat
     4501 gaccatgaat gcaatgcccc aaaccgccgc acaaaacgaa accctgcaca cttgtagcat
     4561 tattgtcgat gatgcgcagg atttttcccc catgcttctc gatgcagggc gggacgacac
     4621 acttatcagc gaatcgatga ttccgcaaat ccgcaccgtt gccgccctga tagccgccga
     4681 acggcacgac ttcagccgca gcagccccgc cgagtttacc gatgccgccg atttttttgc
     4741 cgcacggatt ctcgtattgg gcgtgcgacg cttccatctg gacgtatcgc tgcttcagat
     4801 tttgaaaacc gccaacagcc gcgcccgccg ctttgccgaa aaacaccgcc tgtttttcac
     4861 acccgcacaa gccgaattga gcctgaacaa acacaaaaac cgccgcctgc tgaccgtaga
     4921 aaccgaacac gaagtcgaaa acaaaggcaa cccagtcgcc aacagcctcg catttgtccg
     4981 caaactgcac accctgcccc tgtaaacatc acgatttgtc ggcagaatgc ttgccaaaat
     5041 tcccgacggc gatacaataa aaccgatttt ccacccaaac aggacacaca tgaaaaaaat
     5101 catcgcctcc gcgcttatcg caacattcgc actcgccgcc tgccaagacg acacgcaggc
     5161 gcggctcgaa cagcagcaga aacagattga agccctgcaa cagcagctcg cacagcaggc
     5221 agacgatacg gtttaccaac tgactcccga agcagtcaaa gacaccattc ctgccgaagc
     5281 acaggcaaac ggcaacaacg ggcaacccgt taccggtaaa gacgggcagc agtatattta
     5341 cgaccaatcg acaggaagct ggctgctgca aagcctggtc ggcgcggcgg caggcgcgtt
     5401 tatcggcaac gcgctggcaa acaaattcac acgggcaggc aaccaagaca gtcccgtcgc
     5461 ccggcgcgcg cgtgcagcct accatcagtc cgcacgcccc aatgcgcgca ccagcaggga
     5521 tttgaacacg cgcagcctcc gcgcaaaaca acaggcggcg caggcgcagc gttaccgccc
     5581 gacaacgcgc ccgcccgcca attaccgccg accggctatg cgcggtttcg gcaggaggcg
     5641 gtaaacccgg cgcgccaatg ccgtctgaag ggctttcaga cggcattttg tttccaagag
     5701 tttgatgtcg ggatggcaat tctgatttac gtgcgttggc agggcggcat tgtcatgccg
     5761 ccgttgggtt ttcagacggc atcatatcga accgtcaggt cgtggggtga gcattcttgc
     5821 ccaccgttgt aagggcggga ttttttggtg aacaggaatg cccaccctgc atctgaaacc
     5881 cttctgcgtt attcccacgc aggaaggaat ttagatattt gaatttacag aaattttcaa
     5941 tcgttgccaa agcggaaaag g
//
LOCUS       DAWGJV010000125         5848 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWGJV010000125 DAWGJV010000000
VERSION     DAWGJV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.125"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WHQ94_001670"
     CDS             complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WHQ94_001670"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ2128916.1"
                     /translation="MSVYTSVSDDEMRGFLDDYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1051..1779)
                     /gene="ubiG"
                     /locus_tag="WHQ94_001671"
     CDS             complement(1051..1779)
                     /gene="ubiG"
                     /locus_tag="WHQ94_001671"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ2128917.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1931..3172
                     /locus_tag="WHQ94_001672"
     CDS             1931..3172
                     /locus_tag="WHQ94_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ2128918.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3305..3868
                     /gene="gmhB"
                     /locus_tag="WHQ94_001673"
     CDS             3305..3868
                     /gene="gmhB"
                     /locus_tag="WHQ94_001673"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ2128919.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3898..4641
                     /locus_tag="WHQ94_001674"
     CDS             3898..4641
                     /locus_tag="WHQ94_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ2128920.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETV"
     gene            4638..5327
                     /locus_tag="WHQ94_001675"
     CDS             4638..5327
                     /locus_tag="WHQ94_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ2128921.1"
                     /translation="MTSLIHTLSDGMELTIEIKRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPQTAENRLPESILFHGRQLALTAHQDTQILLMPS
                     EIRVPEGAPEKQLALLQDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWL
                     KIHGRELFALG"
     gene            5439..5624
                     /locus_tag="WHQ94_001676"
     CDS             5439..5624
                     /locus_tag="WHQ94_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128922.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         1371 a         1899 c         1381 g         1197 t
ORIGIN      
        1 aaaatactcg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac
       61 cctttcagac ggcatttctg tcaaacgccg gacgcactca acccaaactc aacagcaggt
      121 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaaat
      181 ccaacaggcg cgacacccaa aaacggatgc agcctgcacg ttgggcggtc gggaaatacg
      241 ccttttcttc ggcactcaag gggcgcacgc cctcataacc gccgataaac gcctttttca
      301 acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
      361 catacataaa attgccccgg caggcataat agaaatcgat gaaacccgat acctgaccgc
      421 cgtcaagcaa cacattgtct ttaaacagat cggcatggat gatgcccgaa ggcagatgat
      481 tgccgagatt gtccttcaac gcatcgattt cggaacacag cagtgcggca tcgtcttgcg
      541 acaggacggg cagcagccgg gcgcacgcct ccgtccacca cgcatcgtaa cgcgggtttt
      601 ccatttccaa agggaaatca gcggcggcaa ggtgcatttt ggccaacatc gcaccggtat
      661 ggaagcactg ctccgccgtc ggcagcgcag tatccgaacc tttcaggcag gcaaccaggc
      721 aggcaggctt gcccgccaaa acggaatcaa gccggccgtc tttgcgcgca accggcgcgg
      781 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
      841 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
      901 aattgctgtt ggtaatcccc tgcgcgatgc cctgcaggga aacaaattct cccaaatcgt
      961 aatcatccaa aaagccgcgc atttcatcat cggaaacact ggtataaaca gacatatcca
     1021 ttcacccgtt caaaacaatc aaacccgccg tcagaacgcc ggacggcagg caaacatata
     1081 attcacatcg gtcgaatcgc acagggcata agtttgcgac aacacatggt aagtcatacc
     1141 cttcgtatcc gccacatcca agcctgcctg acggcacatc cgcgccagct cggcaggcgc
     1201 gatgaatttt ttccagtcgt gcgtaccttt ggggacgaac ttcaacagat attccgccgc
     1261 cacaatcaga tgcaggtacg atttcgggtt tttattgatg gtggaaaaaa acaccatgcc
     1321 gtccggtttg accagcttgg cacaggcgcg aacgatggcg gcgggatcgg ggacgtgttc
     1381 catcatttcc atgcacgtta ccacatcgaa cgagtgcggt tccgcctcgg caaggtcttc
     1441 cacgcggata cattcgtatt cgatatcggc gacattgttc aaagccgcgt gcaggcgggc
     1501 ggtttccagc gactgctccg ccatgtcgat accctttaca aacgccgcgc cgcgccgcgc
     1561 catactttcc gccaagatgc cgccgccgca gcccacatcc aaaacccgtt tgccgcgcaa
     1621 atccgcgtgt ccgtcgatat aatccagccg cagcggattg atgtcgtgca aggttttgaa
     1681 ctcgcccgac ttgtcccacc atttgtcggc aatccggctg aatttggcga tttccccctc
     1741 atcgacatta tattttttgt cggacatttt ccctcccatc tgacgaaccg cccactccaa
     1801 aacccaagat tatactatgg aacgcaatgc cgtctgaaag ccttttcggg cggcgcggcg
     1861 caaatacctt acaaatcctt acactttacg gcataatggc ggctcgcttt ttctggcaga
     1921 aagacaaaat atgcccacca aaaccccttc actgttcggc ggcgcgatga ttatcgccgg
     1981 cacggtcatc ggcgcaggta tgctcgccaa cccgaccgcc acatccggcg tatggtttac
     2041 cggctcgctg gccgtgttgc tgtacacctg gttttccatg ctctccagcg gcctgatgat
     2101 tttggaagtc aacacccact acccccacgg cgcgagcttc gacaccatgg ttaaagacct
     2161 gctcggacgg agctggaaca tcatcaacgg catcgccgtc gccttcgttt tatacctgct
     2221 tacttacgct tatatcttcg tcggcggcga cctgaccgcc aaaggcttag gcagcgcggc
     2281 aggcggcaat gtttcactca ccgtcggaca actcgtcttc ttcggcattc tcgccttttg
     2341 cgtatgggca tccgcacgct tggtcgaccg attcaccagc gtcctcatcg gcggcatggt
     2401 attaaccttt atttgggcaa ccggcggcct gattgccgat gccaaactgc ccgtcctctt
     2461 cgacacccaa gcccctaccg gcaccaacta ctggatttat gtcgccaccg ccctgcccgt
     2521 ctgccttgcg tcattcggtt tccacggcaa cgtctccagc ctgctcaaat actttaaagg
     2581 cgacgcgccc aaagtggcta aatccatctg gacgggcaca ctgattgcgc tggtaattta
     2641 cgtcctctgg caaaccgcca tccaaggcaa cctgccgcgc aacgagttcg cccccgtgat
     2701 tgccgccgaa gggcaagtct ccgtcctgat tgaaaccctg tccaaattcg cccaaaccgg
     2761 caatatggac aaaatattgt ccctgttttc ctatatggcg atcgccacct cgtttttagg
     2821 cgtaacgctc ggactcttcg actacatcgc cgacatcttc aaatggaacg acagcgtgtc
     2881 cggccgcacc aaaaccgccg cgctgacctt cctgccgcct ctaatttcct gcctgctctt
     2941 ccccaccggc tttgttaccg ccatcggcta cgtcggcctg gcggcaaccg tctggacagg
     3001 catcatcccc gccatgctgc tctaccgttc gcgcaaaaaa ttcggcgcag gcaaaaccta
     3061 taaagtttac ggcggcttgt ggctgatggt ttgggtcttc cttttcggca tcgtcaacat
     3121 cgccgcacag gtattgagcc aaatggaact cgtccccgta tttaaaggat aaaggcaaaa
     3181 tgccgtctga agcccgccgg cggcttcaga cggcattgcc tcgttatcac gcggcacagg
     3241 cgcatcactg ccgatatttc agtataatgc cacccccgac ctgccccaat ccaaaggaaa
     3301 cgcgatgaaa ctcatcattc tcgaccgcga cggcgtcatc aatcaggacc gcgatgactt
     3361 cgtcaaatcc gttgacgagt ggatacctgt cgaaggcagc atggatgcgg tggcatttct
     3421 gacgcaggca ggctacaccg tcgccgttgc caccaaccaa tccggcatcg ggcgcaaata
     3481 ttttaccgtt caaaacctta ccgaaatgca cgccaaaatg caccgcctcg tccgtcaggc
     3541 aggcggcgaa atcaacggca tctggttctg cccgcacacc gatgccgaca actgcaactg
     3601 ccgcaagccc aaaccgggta tgattgaaga catcatcgga cgcttcaacg cccaagcctc
     3661 ggaaacctgg ctggtcggcg acagcctgcg cgatttgcag gcaatcgatg ccgtcggcgg
     3721 aaaacccgcg ctggttctga ccggaaaagg caaaaaaacg ctctcccaac acggacacga
     3781 attacccgaa cacacacagg ttttcgatac cttgctcgat ttctcacaat acatcatgca
     3841 ggaaaacgcc gcaccgcaag ccgactgaac ataccgcatt tccgacaagg caaaaccatg
     3901 ctcatcatcc gcaacctgat ttactggctg atactctgtt ccaccctgat tttcctcttt
     3961 ccctttatgc tgctcgcctc gcctttccgg gacggggcgc acaagatggc gcgggtctgg
     4021 gtcaaaatcc tcaacctctc gctcaaacac atcgtcgggc tcaaataccg catcatcggc
     4081 gcggaaaaca tccccgaccg ccccgccgtc atctgcgcca aacaccaaag cggctgggaa
     4141 acgcttgccc ttcaggacat ttttccgccg caggtttacg ttgccaaacg cgagctgttc
     4201 aaaatcccct ttttcggctg gggtttaaaa ctggtcaaaa ccataggcat agaccgcaac
     4261 aaccgccgcg aagccaacga acagctcata aaacaggggt tggcgcgcaa aaacgaaggc
     4321 tattggatta ccattttccc cgaaggcacg cgccttgcgc ccggaaaacg cggcaaatac
     4381 aaactcggcg gcgcgcgcat ggcgaaaatg tttgagatgg acatcgtccc cgtcgccctc
     4441 aacagcggcg aattttggcc gaaaaactcc tttctgaaat atccggggga aatcaccgtc
     4501 gtcatctgtc cgaccatccc gcacgcaagc ggcagcgaag ccgaattgat ggaaaaatgc
     4561 gaacacctca tcgaaacgca gcagccgctc atttccggcg caggcccgtt tgccgccaaa
     4621 atgccgtctg aaaccgtatg acctccctga tccacactct ttctgacggc atggaactga
     4681 ctatagaaat caagcgccgt gccaagaaaa acctgattat ccgccctatc ggcacacata
     4741 ccgtccgcat cagcgtccca ccctgcttct ccgtctccgc tctaaaccgc tggctgtatg
     4801 aaaacgaagc cgtcctgcgg cgaacactgg cgaaaacacc gccgcaaact gccgaaaacc
     4861 ggctgcccga atccatcctc ttccacggca gacaacttgc cctcaccgcc catcaagaca
     4921 cgcaaatcct gctgatgccg tctgaaatcc gcgttcccga aggcgcaccc gaaaaacagc
     4981 ttgcgctgct gcaggacttt ttggaacggc aggcgcacag ttacctgatt ccccgcctcg
     5041 aacgccacgc ccgcaccaca caactgttcc ccgcctcctc ctcgctgacc tctgccaaaa
     5101 ccttctgggg cgtgtgccgc aaaaccacag gcatacgcct caaccggcgg ctggtcggcg
     5161 caccggaata cgttgccgat tatgtctgca tacacgaact ctgccacctc gcccatcccg
     5221 accacagccc cgccttttgg gaactgaccc gccgcttcgc cccctacacg cccgaagcga
     5281 aacagtggct caaaatccac ggcagggaac ttttcgcctt aggctgacgc ggtcccgggc
     5341 cggcatccgc caattcagac ctcctcccaa acggacatcc ggaaagcagc atcacagccg
     5401 gcgacacata ccgctgccgc cgctttcaga cggcatccgt gccggaacag gcacgcgccc
     5461 gcccgattca aaccgcgatg acgctttgcc gccggttcgg ggcaggatgg cggcacacgc
     5521 gccgtctgcc gcgtttcatt tcacaccgct cttccgaaac ccgaaacccg cccggtccga
     5581 cgtgcggtat gaaacgctta agctggcgcg aagtctttta ctgatttgcc cgcgaaaatg
     5641 ccgtctgaaa ggttttcgga cggcattttt ttgcgtttcc caggatggcg gcggattcct
     5701 aaaaggcggt cagggtggat tgtaggatgg gttgagacct gccgaatccg ccgcatctgc
     5761 caaatctacc gccgtcattc ctacgaaagt gggaatctag aacgcggggt taagaaaacc
     5821 tgcatcccgt cattcccacg aaagtggg
//
LOCUS       DAWGJV010000126         5666 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWGJV010000126 DAWGJV010000000
VERSION     DAWGJV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.126"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            284..1231
                     /locus_tag="WHQ94_001677"
     CDS             284..1231
                     /locus_tag="WHQ94_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ2128923.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="WHQ94_001678"
     CDS             complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="WHQ94_001678"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ2128924.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2506..3309
                     /locus_tag="WHQ94_001679"
     CDS             2506..3309
                     /locus_tag="WHQ94_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ2128925.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMIKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3367..5586)
                     /locus_tag="WHQ94_001680"
     CDS             complement(3367..5586)
                     /locus_tag="WHQ94_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ2128926.1"
                     /translation="MFRRWFLPCWVFGAVASFALPSVPHWPFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     EDDGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1352 a         1806 c         1373 g         1135 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgccata aacagacggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggccatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggcc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatgacctat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggcg aaatgctgga aatccaagcg gccggtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacga aaccggcggt ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cagggacgat tgtccggctg ctccgattcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctactccc ttacataaac cacttcgaca tcgtagtcgt cttcgttcca atcgtcatcg
     1441 tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc tcaaagacga atccaaaccg
     1501 gcttcaagac ggaggacaga caacaaatga tatatgtcgt ccggaatcgg tgcctcaaac
     1561 gaaacggttt cgccgctttc cggatgggtg aaactcaagc ggtaggcgtg caacgcctga
     1621 cgcgcgccca aacttttaac ggcttctttc accgtgtcgc cgcacggatg gcgcgggttg
     1681 ccgtaaaccg ggtcggcggc aagcggatgg ttggcctcgc gcatatggac gcggatttgg
     1741 tgcgtcctgc ccgtttcgag cgagcattcg atatagctgt gggtaagata gcgttccaac
     1801 actttgacgt gggtaacggc tggtttgccg ccgaatttga cgactgccat tttcaggcgg
     1861 ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt cgaaggggac gatgccgttg
     1921 gcgacggcgc ggtagatgcg tttgaccgtg cgctcttgaa gctgcctcac gagggaattt
     1981 tgcgccggca gggttttggc aaccaccatc agcccgctgg tttccttgtc caaacggtgt
     2041 acgatgcccg cgcgcggtac ttggctcaat tcgggacagt gcgccaacag gccgttgagc
     2101 agcgtccccg tccagttgcc cgccgccgga tgcaccacca gtccggccgg tttgttgacg
     2161 acgatgacgg tatcgtcttc gtaaacaata tccaaagcca taggctctgg aacaaacgcc
     2221 agattttcct cactcggacg gacggttaca caaatttgct cgccgcctat cattttgtct
     2281 ttgggttgcg aaggtttatc gtttacaata accgcgcctt ctttaatcca tgatgtcagg
     2341 cggctgcgcg agtagtcggg cagaagtttc gccaacaccg catccaaccg cccgccggca
     2401 agctcgagcg gaacggtcag accgatacgg ttttcagttt cggaagctga cgcaaagtct
     2461 gaatcgtcgc tataatcgga ttcattatca aaggaagtat tctgcatgaa aaaaattctt
     2521 ttaacggttt cattaggttt ggcactgagt gcctgtgcca ctcaaggtac ggtcgataaa
     2581 gatgctcaga ttacccaaga ttggagtgtg gagaagctct atgccgaagc ccaggacgaa
     2641 ttgaacagca gcaattatac gcgggctgtc aagttatacg aaatcttgga atcgcgcttc
     2701 cccaccagcc gccatgcccg gcagtcgcaa ctggataccg catacgccta ttataaagac
     2761 gatgaaaaag acaaagctct ggcggcaatc gaacgcttcc gccgccttca cccgcaacac
     2821 ccgaatatgg attacgcgct atacctgcgc ggtttggtac tgttcaacga agatcagtct
     2881 ttcttgaaca agctggcctc gcaagactgg tccgaccgcg atccgaaagc caaccgcgaa
     2941 gcgtatcagg cctttgcgga actcgtccaa cgcttcccga acagcaaata cgccgccgat
     3001 gcgaccgcac gcatgatcaa actggtcgat gctttgggcg gcaatgaaat gtcggtggcg
     3061 cgctactaca tgaaacgcgg tgcatacatc gccgccgcca accgcgccca aaaaatcatc
     3121 ggcagctacc agaatacgcg ttatgtcgaa gaatcgctcg ccatattgga acttgcctac
     3181 caaaaactcg gcaaaccaca gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc
     3241 ccgaaaagcc cgtttttgac gcacgcttgg cagcccgacg atatgccctg gtggcgttac
     3301 tggcattaag cccatcttcg taatgccgtc tgaagccgtt tcagacggca ttttatgttt
     3361 caaacctcat tcaaacggtt ttttctgcca atagaattta taccctctca aacgttgagc
     3421 cttcacgccg ccgcgcccca agccgaattg cagtgcgccc gacaaatcgg tacgcagcag
     3481 tttgatgccg tgtgcgcgga cacggttctg caccgcttcg gtcggatgtt tgtaggcgtt
     3541 ggcataaccg cttgaagcaa cggcatattc gggcgagacg gcattgagga acacgcccga
     3601 cgaggacgta ttgctgccgt gatgtcccaa caccaaaacc tggctgtaca ggttgcctcc
     3661 atacttgccg accaggcttt cctcgccctt cgtatccaaa tcgcccgtta ccagcagtgc
     3721 cgcaccgccc gccacaacac gcaaaacaca acttttccca ttatcatcga tgtttttgcg
     3781 ttcagacggc atcaaaaact cgaaatctac gccgtcccat tgccaacgct gttccgcaca
     3841 atgccgcgcc ccctcataaa attccggctg tccggcataa atcccgccgt tcggtatctt
     3901 gccgactgcc tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacagaa ccagcttgtc
     3961 caaacggcgg acacccgccg cattcaaact cggcacaatc cccatctgcg ccgccgctgc
     4021 cgttccggtg tcaaacaaaa gatgatgatt tgccgtctga accgacaccg acaaaccctg
     4081 ccccgcatcc caaaccgtaa ccgcaacctc attttccggc acgccgggtg aacggtaaaa
     4141 cacaaaccct gccaacagca acaccgccca cggacgcaaa cccaagccgc gcggcagcag
     4201 caacagcaaa gcggcacaca ccgccaacac caacagcggc aaaggtgcgg aggcaacggc
     4261 aaactcgggc gacacatcgg caagccacac caaaaaccgc aaagtatatt ccgccaaaaa
     4321 tgcccccaac tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg ccagcggcgt
     4381 caatacccaa gaaaaccacg gaatcgccac cgcattgacc aaagggctga ttaaaggcag
     4441 cgaagcaaac agataaccga gcaaaaccaa cgacaacacc gaagccgccc actgcccgcg
     4501 caaagcagtt tgccgtttcc cctcgtgcaa acgccccgaa cacgcccata tcagggccgc
     4561 caccaaacca aaagacagcc aagtccccac acccaagacc gccaaagggt cgaacagcag
     4621 caccgccgcc aacgcctgcc accacgttgc ccacgccgac agtcttcccc tgcgccaagc
     4681 ccacgcaaac gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa aaccggcaag
     4741 cagcgcgtaa aacagcgcgc ctgcacaccc cgccgccaaa acccacacgc gcggccgggc
     4801 aggaaggcgc ggggaacagg caagcagccg cttcgccagc cacgcaaaca acaccgccac
     4861 catcgtaacg tgcaaacccg aaatactgac caaatgcgtc agccccaacg gtcggaacgc
     4921 ctgccacaat tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc gcatcagccc
     4981 gattccgtct gaaagcccgc cgtccgcatc cgcctgccgc caattacggc tgatgcggct
     5041 gcgccaaacc gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt ccgcaccgac
     5101 cgtccccgcg ccgcctattc cgttggataa tgcccacgcc tcacggttca aaccgcgcag
     5161 gttcaactcc ccgacgacag ggtgcacacg tgccgttatc cgccatctgc tcccgaccgc
     5221 ccattcgcgc cgtttgtagt ccgacagcag caaatcgaac gtccgaccac cgtcatcctc
     5281 cgcctttgcc gcaaactgca cgcgccgccc gtccgacctc ggcatatccg ccacttccac
     5341 tgtcaaaggc acgcctgaaa ccgcctccgc ccaccattgc gaagacagtg ccgcttccgt
     5401 tctgaatacg ccgtaagccg cccccgccaa cacacacaac attagaccgg caaacgcaaa
     5461 ccgccttgca aacacagcaa aaaccgcaaa agccgccaac caaaacggcc aatgcggcac
     5521 ggacggcagc gcgaacgaag ccaccgcgcc gaagacccaa cacggcaaaa accaacgacg
     5581 gaacacgtct ccctacccca gatatttcaa aaataaacgg ataataaagc aaatccgaaa
     5641 aaccgtcata acaagatttg aaaatg
//
LOCUS       DAWGJV010000127         5629 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWGJV010000127 DAWGJV010000000
VERSION     DAWGJV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.127"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WHQ94_001681"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WHQ94_001681"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ2128927.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEQAIVKATFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            1137..3002
                     /gene="msbA"
                     /locus_tag="WHQ94_001682"
     CDS             1137..3002
                     /gene="msbA"
                     /locus_tag="WHQ94_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ2128928.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3102..4667
                     /gene="guaA"
                     /locus_tag="WHQ94_001683"
     CDS             3102..4667
                     /gene="guaA"
                     /locus_tag="WHQ94_001683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ2128929.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMEKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4729..5475
                     /gene="fabG"
                     /locus_tag="WHQ94_001684"
     CDS             4729..5475
                     /gene="fabG"
                     /locus_tag="WHQ94_001684"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ2128930.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1367 a         1797 c         1371 g         1094 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gcattcttct ttcccggaca aggctcacaa agcctaggca
      121 tgatgaacgg ctttgccgag caagccatcg ttaaagccac atttgacgaa gcttccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggtcaaa
      241 ccgtcaacac ccagcccatt atgctcgccg ccggcgttgc cgtttaccgc gcctatttgg
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg aattttgccg acgcggtcaa actcgtgcgc ctacgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcaatt ttgggtttgg
      481 aagatgaaca ggtaaggcag atttgcaccg aatccgcaca ggatgaagtg gttgaagccg
      541 tcaacttcaa ctcgcccgga caagtcgtaa ttgcaggcaa tgccgccgcc gtcggacgcg
      601 ccatgaccgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctcatg aaacccgccg ccgacaaact cgccgaagcc ctcaaaaccg
      721 tcgaaatcaa gcagccgcaa atccgcgtca tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgcccttgtt tcagacggca ttgccgaatc cgccgaatgc ggaccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaaaccgc cgcgtgcagc gcgctgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cgcactgact tcgttccgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcacgtccga acggcaaaca gccgcatatt tacgctataa
     1081 tccatcccga ccaaaccacc gacagcggct gccgttgcag tttccgcccc accgatatga
     1141 tagaaaaact gactttcgga ctgtttaaaa aagaagacgc gcgcagcttt atgcgcctga
     1201 tggcatacgt ccgcccctac aaaatccgca tcattgccgc cctgattgcc attttcggcg
     1261 ttgccgccac cgaaagctac cttgccgcct tcatcgcccc cctgattaac cacggctttt
     1321 ccgcacctgc cgcgccgccc gagctgtctg ccgccgccgg catcatttcc accctgcaaa
     1381 actggcgcga acagtttacc tatatggttt gggggacgga aaacaaaatc tggaccgtcc
     1441 cgctcttcct catcatcctc gtcgtcatcc gtggcatctg ccgctttacc agcacctatc
     1501 tgatgacttg ggtctccgtg atgaccatca gcaaaatccg caaagatatg tttgccaaaa
     1561 tgctgaccct ttcctcccgc taccatcagg aaacgccgtc cggcaccgta ctgatgaata
     1621 tgctcaacct gaccgaacag tcggtcagca acgccagcga catcttcacc gtcctcacgc
     1681 gcgacacgat gatcgttacc ggcctgacca tcgtcctgct ttacctcaac tggcagctca
     1741 gccttatcgt cgtcctgatg ttccccctgc tctccctgct ctcgcgctac taccgcgacc
     1801 gtctgaaaca cgtcatttcc gactcgcaaa aaagcatagg cacgatgaac aacgtgattg
     1861 ccgaaaccca tcagggacac cgcgtcgtca agctgttcaa cgggcaggcg caggcggcaa
     1921 accggttcga cgcgatcaac cgcaccatcg tccgcctcag caaaaaaatc acgcaggcaa
     1981 cggcggcaca ttccccgttc agcgaactaa tcgcctcgat cgccctcgcc gtcgtcatct
     2041 tcatcgccct gtggcaaagc caaaacggct acaccaccat cggcgaattt atggcattca
     2101 tcgtcgcgat gctgcaaatg tacgccccca tcaaaagcct tgccaacatt agcatcccta
     2161 tgcagacgat gttcctcgcc gccgacggcg tatgtgcatt tctcgacacc ccgcccgaac
     2221 aggacaaagg cacgctcgca ccgcagcgtg tcgaagggcg catcagcttc cgcaacgtcg
     2281 atgtcgaata ccgttcggac ggcatcaaag ccctcgacgg cttcaacctc gacatcagac
     2341 aaggcgaacg cgtcgccctg gtcggacgtt ccggcagcgg caaatccacc gtcgtcaacc
     2401 tgctgccccg ctttgtcgaa ccgtctgccg gcaacatctg catagacggt atcgacatcg
     2461 ccgacatcaa actcgactgc ctgcgcgccc aatttgccct cgtctcccaa gacgtattcc
     2521 tgtttgacga caccctgttt gaaaacgtcc gatatagccg tcccgacgcg ggcgaagccg
     2581 aagtcctatc cgccctccaa gccgctaacc tgcaaagcct gatcgacgca tcaccgctcg
     2641 gactgcacca gcccatcgga tcgaacggca gcaacttatc cggcggacag cggcaacgcg
     2701 ttgccattgc ccgcgccatt ttgaaagacg cgccgatatt attattggac gaagccacca
     2761 gcgcattgga caacgaatcc gaacgcctcg tccaacaggc gctcgaacgc ctgatggaaa
     2821 accgcaccgg catcatcgtc gcccaccgcc tgaccaccat cgaaggggcc gaccgcatca
     2881 tcgtaatgga cgacggcaaa atcatcgaac aaggcacaca cgaacaactg atgtcccaaa
     2941 acggctacta cacgatgtta cgcaatatct caaacaaaga tgccgccgtc cggacggcat
     3001 aaacaaaatg ccgtctgaaa tggtacaatc gccccgaccc tttcagacgg catcatatcc
     3061 gccgacccat ccgattatct tcaatcaccg taaaacccat tatgacccaa gacaaaatcc
     3121 tcatcctcga cttcggttct caagttaccc agctgattgc ccgccgcgtg cgcgaagccc
     3181 acgtttactg cgaactgcat tccttcgata tgcctttgga cgaaatcaaa gccttcaacc
     3241 ccaaaggcat catcctttcc ggcggcccta attccgttta cgaatccgac tatcaggccg
     3301 ataccggtat ttttgatttg ggcattccgg ttttgggcat ctgttacggc atgcagttta
     3361 tggcgcacca cttgggcggc gaagtgcagc ccggcaacca gcgcgaattc ggttatgcgc
     3421 aagttaaaac catagacagc gagctgacac gcggcattca agatggtgag ccaaacacac
     3481 tcgacgtatg gatgagccac ggcgacaaag tgtccaaact gcccgacggt ttcgccgtca
     3541 tcggcaacac cccgtcctgc ccgattgcca tgatggaaaa cgccgaaaaa caattctacg
     3601 gcatccagtt ccaccccgaa gttacccaca ccaaacaagg ccgcgccctg ttgaaccgct
     3661 ttgtcttgga tatttgcggc gcgcaaccta gctggacgat gccgaactac atcgaagaag
     3721 ccgttgccaa aatccgcgaa caggtcggca gcgacgaagt gattttgggt ttgtccggcg
     3781 gcgtggactc ttccgtagcc gccgcgctga ttcaccgcgc catcggagac caactgacct
     3841 gcgtgttcgt cgatcacggc ctcttgcgct tgaacgaagg caaaatggtg atggatatgt
     3901 tcgcccgcaa tttgggtgtg aaagtgattc acgtcgatgc cgaagggcag tttatggaga
     3961 aactcgccgg cgttaccgat cccgagaaaa aacgcaaaat catcggtgcg gaatttatcg
     4021 aagtatttga tgctgaagaa aaaaaactca ccaacgccaa atggctggcg caaggcacga
     4081 tttaccctga cgtaatcgaa tccgcaggcg cgaaaaccaa aaaagcccac gccatcaaat
     4141 cccaccacaa tgtcggcggc ctgcctgaaa acatgaagct caagctgctt gagccgttgc
     4201 gcgatttgtt caaagacgaa gtgcgcgaac tgggcgtggc tttgggtctg ccgcgcgaaa
     4261 tggtgtaccg ccatcccttc cccggtcccg gtttgggcgt gcgcatcttg ggcgaagtaa
     4321 aaaaagaata tgccgacctg cttcgtcagg cagacgatat ttttattcaa gaattacgca
     4381 acaccaccga cgaaaacggc acgtcttggt acgacctgac cagccaagcc ttcgccgtgt
     4441 tcctgcctgt gaaatccgtc ggcgtaatgg gcgacggacg cacttacgac tacgtcgtcg
     4501 cactgcgcgc agtcatcacc agcgacttta tgaccgcaca ttgggctgag ttgccttact
     4561 cactgctcgg acgcgtgtcc aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg
     4621 tgtacgatgt cagcggcaaa ccgcctgcca ccatcgagtg ggaataacca gcaaacaggg
     4681 ctgcaccgtc cggcgcagtc tttcgattat cggaaaaaag gaaaaaatat gagcacacaa
     4741 gatttaagcg gcaaaatcgc tttggtaaca ggcgcctcgc gcggtatcgg tgcagcaatt
     4801 gccgacacac tggcggcagc cggtgccaaa gtcatcggta cggcgaccag tgagagcggt
     4861 gcggcggcga ttagcgagcg gttggcgcaa tggggcggcg aaggccgcgt attaaattcc
     4921 gccgaacccg aaaccatcga aagcctgatt gccgacatcg aaaaagcatt cggcaaactc
     4981 gacattctgg tcaacaacgc cggcatcacc cgcgacaacc tcctgatgcg catgaaagaa
     5041 gaagagtggg acgacatcat gcaggtcaac ctaaaatccg tgttccgcgc ttctaaagcc
     5101 gtcttgcgcg gtatgatgaa acaacgttcc ggccgcatca tcaacatcac ttctgttgtc
     5161 ggcgtgatgg gcaatgccgg tcaaaccaac tatgccgcgg caaaagcagg cttaatcggt
     5221 tttgccaaat ctatggtgcg cgaagtcggc agccgcggta ttaccgtcaa ctgcgtcgcc
     5281 cccggcttta tcgataccga catgacccgc gccctgcccg aagaaacccg ccaaaccttt
     5341 accgcccaaa ccgccttggg cagattcggc gacgcgcaag atattgccga tgcggttctg
     5401 ttcctcgctt ccgatcaggc aaaatacatt accggccaaa cgctgcacgt caacggcggc
     5461 atgttgatgc cctgacggac agccgtgccg acaatgccgt ctgaagccct ttcagacggc
     5521 atttgcattc tcaggcaaaa tgaacacacg ccacaccccg ccctgcccct gcggctcagg
     5581 cacaagctga gacctttgca aaactccttt ccctcccgac agccgaaac
//
LOCUS       DAWGJV010000128         5484 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWGJV010000128 DAWGJV010000000
VERSION     DAWGJV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.128"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            65..490
                     /locus_tag="WHQ94_001685"
                     /pseudo
     CDS             65..490
                     /locus_tag="WHQ94_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            496..1725
                     /locus_tag="WHQ94_001686"
                     /pseudo
     CDS             496..1725
                     /locus_tag="WHQ94_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1783..2403
                     /locus_tag="WHQ94_001687"
     CDS             1783..2403
                     /locus_tag="WHQ94_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128931.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2414..2545
                     /locus_tag="WHQ94_001688"
                     /pseudo
     CDS             2414..2545
                     /locus_tag="WHQ94_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2542..3036
                     /locus_tag="WHQ94_001689"
     CDS             2542..3036
                     /locus_tag="WHQ94_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ2128932.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGSPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3124..4785)
                     /locus_tag="WHQ94_001690"
     CDS             complement(3124..4785)
                     /locus_tag="WHQ94_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ2128933.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQVIERFQLDQNSQPQTYGLGIKE
                     IWEVPSEKHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4915..5457)
                     /locus_tag="WHQ94_001691"
     CDS             complement(4915..5457)
                     /locus_tag="WHQ94_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ2128934.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1309 a         1478 c         1398 g         1299 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatt gatacgctga tggcgttggc
      421 taaagacggt acgatagccg gagcgaaatt ggttgatcac catgagtcga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc tcaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttct
      601 gcaaccatcg aaggggacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggc atgagcctgc cagcaatgga tgccggtatt
      721 tatctcgaag aaaccgctcc gcacgccctg attgaacaca ataatatttt ggataattcg
      781 gtcggcgtct atctgcatgg ttcttccgaa gcgatggtgc gcgagaacaa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcgcccggt
      901 gcgcaagtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caacaccagc
      961 acatataaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
     1201 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
     1321 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtatcgcgtc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgagt tgctcaaaga agccgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac gggtctttga actagtctgt ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgttaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gcagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 actgttgcgt tgcacccttc gctgaccggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaatcggggg ctgctcgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg cctcgaccct
     2281 gcatcaaggc aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagta cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
     2401 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
     2461 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
     2521 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
     2581 ttaagtgcct gccggcaggc ggaagaggga tcgccgcctt taccccggca gattagcgac
     2641 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
     2701 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
     2761 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
     2821 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
     2881 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
     2941 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
     3001 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
     3061 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
     3121 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
     3181 ttgcgtcggg tctttgatat cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
     3241 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
     3301 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
     3361 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
     3421 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttgcacg cggcggcttt
     3481 tttgagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
     3541 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
     3601 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
     3661 ataatccgcc gcctctttgc cgctgtcgaa gttttccact tcttcaaggt tttccaacaa
     3721 ggggaacacg gcttctgcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
     3781 gcgcggcata ttgaggaagc ctgccgcgtc gccgattaaa acgccgcctt tgaacgagag
     3841 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag cgatgcggcg
     3901 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
     3961 cggcgacaga taagggtttt gatagtccaa accgaccacg aaaccgacgg cgactttgtt
     4021 gtcatcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcccgc
     4081 gctgtgcacc accaagccgg gctgatgttt ttcagacggc acttcccaaa tttctttgat
     4141 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgacttg
     4201 tttggaaagc gaaccgcgac aaccttcggc aaacagagtt tgctgcgccc aaagctccat
     4261 gccgggctgg aaactgtcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
     4321 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
     4381 aatttccacg cccaaatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
     4441 gacgatgtag ttgccgtgat tgtcgaaatt tggggtaata ggcagattga acgctttttt
     4501 ctcagtcagg aacaacactt tgtcctgcgt taccgtacgc gtcagcggcg cgcctttttc
     4561 tttccagtcg ggaatcaact cattcagcgc aatcggatcg ataaccgcgc ccgccagcga
     4621 atgtgcaccc gcctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccattttg
     4681 ttcggcaagc tgcttgagtt tgatggcggc ggacaaaccc gatgggcctg cgccgacaat
     4741 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
     4801 tttattattg aattgcaaat ccgtaattat acaacgagaa catatagtta ccaaatacaa
     4861 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
     4921 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
     4981 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
     5041 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcattgaaac
     5101 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
     5161 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
     5221 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
     5281 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
     5341 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcacc accgaagccg ccccgcagga
     5401 aaaatccaaa tcctgcttta caatattgaa atcgcgcctt tctttccaac tctgcacttt
     5461 gatttttcca taagcaacag gatt
//
LOCUS       DAWGJV010000129         5462 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWGJV010000129 DAWGJV010000000
VERSION     DAWGJV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.129"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(215..2011)
                     /locus_tag="WHQ94_001692"
     CDS             complement(215..2011)
                     /locus_tag="WHQ94_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ2128935.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2468..3721
                     /locus_tag="WHQ94_001693"
     CDS             2468..3721
                     /locus_tag="WHQ94_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ2128936.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYTEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDVGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3876..5387
                     /gene="lysS"
                     /locus_tag="WHQ94_001694"
     CDS             3876..5387
                     /gene="lysS"
                     /locus_tag="WHQ94_001694"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ2128937.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1176 a         1395 c         1601 g         1290 t
ORIGIN      
        1 cgcgccttag ctcaaagaga acgattctct caggtgctga agcaccgagt gaatcggttc
       61 cgtactattc gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
      121 atttgcttgc ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa
      181 gggctttcag acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt
      241 tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg
      301 cgtggtagcg gttgacccag tcgatttcgc cgtcggtcat gagggcggtg tccatcaggc
      361 gggtgtcgat ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt
      421 cttgaggggc ggcgacggct tggttggcgg caaggttttc aatgcggatg ccccattttc
      481 ccgggcggta gagtccgggt tcgatggagg taaccatgcc ttttttcatg gcggtttcgg
      541 gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc
      601 ctacgccgtg tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc
      661 aaatcgcatc aatcagcggc gaggggatgt tttcggggaa cacggcttcg gcaagcgcga
      721 tatgggcttt gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag
      781 ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagca
      841 aaagcccgtt gccgctgatg gtgctgtggc tttcgggtgt cgcgctgtaa tgcggcagtg
      901 cgccgttggc gttgaagcct gcgatggtgt cgaaactcaa tgaaatgaag cctgggcgca
      961 cgctgcggtg gcgataaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc
     1021 cgatgatgtc ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg
     1081 cttcgcggat gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt
     1141 tgattccctc gataaggcgc acgctttcgg gcaggcgcac aagcgtgctg acggcggttt
     1201 tgttcggttc gataagcaac gcgccgccga tttgcgcgag tttgtcggca acttgggcgt
     1261 aaggctcgac cgtgatgcct gcggtttgca gcgcggcggc ggcttcggcg ttcagacggc
     1321 atcggtcggt aaacaggacg gcgttgtctt tgccaatcag caggaaggac acgaaaacgg
     1381 ggttgaaagg cacgtcgctg ccgcgcaggt tggtcagcca ggcgatgtcg tcaagcgagg
     1441 aaaccaagtg gtaatccgcg cctttttccg ccatgacggc gcgcacgcgg gcgagttttt
     1501 cggcggcggt ttcggagacg taggcggggt cgtggatgaa cacggtttcg gcggggatag
     1561 cgggacggct tgtccacact tggtcaagca gatcgtttgg gtgttcgatg cggatgtttt
     1621 tggcggtcag ggattgcgcc aaagtgcgtt tgccggtgag cgagaccata tcggaaggga
     1681 tgccgacggc ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt
     1741 gcccgctttt ttgcagttcg atgccgctgc ccgcaagctg cttggcggct tgttcccaat
     1801 agcggctgtc cacccatacg cccgcttcat cggcggtaac gacgaatgtg ccgaccgagc
     1861 cggtaaagcc cgacaattcg cggcgcgcct gccagtgtgc gggcaggtat tcggacaggt
     1921 gggggtcggc ggaagggatg acgagtgcgt ccaagccttg cgccctcatg gcttcgcgca
     1981 atgcggacag gtaattcgat acggtattca taatagttcc tccaatcggg ttttgcggct
     2041 tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat
     2101 cgccagcctg ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaagcg
     2161 atgtaaggtt gtttgttgta aaattttagg cataaaatgt cgtttcatgc ctttttttgg
     2221 aagtaatatg gaaaatatag tcaagtttga gcatgaagtt ctaatagtgt gtgttggcag
     2281 gtatttggat tacgttacaa tacgcgcttt catcaaaatt tccctaccgg gcggtatcct
     2341 gtttttgaag acggtaggat ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa
     2401 tagatagggc tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga
     2461 agactttatg tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattgggtcc
     2521 ggggatcatg atggcttcgg cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc
     2581 gggcgcgctt tacggctggc agatcgcgct catcatcatc ctgaccaacc tcttcaaata
     2641 cccgtttttc cgcttcagcg cgcattacac gctggacacg ggcaagagcc tgattgaagg
     2701 ttataccgag aaaagccgcg tttatttgtg ggtattcctg attttgtgca tcctctccgc
     2761 cacgattaac gcgggcgcgg tcgccattgt aaccgccgcc atcgtcaaaa tggcgattcc
     2821 ctcgctgatg tttgatgtcg gcacggttgc cgccttgatt atggcatcct gcctgattat
     2881 tttggtgagc ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt
     2941 gagtatcgcc acgcttgctg ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc
     3001 cgattttatc gagccgacac cgtggacgct tgccggtttg ggcttcctga tcgcgctgat
     3061 gggctggatg cccgcgccga ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa
     3121 acaacgcatc aatccttccg aataccgcga cgggattttt gatttcaacg tcggttatat
     3181 cgccagtgcg gttttggctt tggttttcct tgcactgggt gcgtttgtgc aatacggcaa
     3241 cggcgaagca gtgcagatgg cgggcggcaa atatatcggg caattgatca atatgtacgc
     3301 cgttaccatc ggcggctggt cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta
     3361 cggcacgacg attaccgtcg tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct
     3421 gctgcgcgga aaagacaaaa cgggcaacgc cgaattcttt gcctggaata tttgggtggc
     3481 gggcagcggt ttggcggtga ttttttggtt tgacggcgta atggcgaatc tgctcaaatt
     3541 tgcgatgatt gctgcttttg tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt
     3601 caaaggtgat gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt
     3661 gatttatctg accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg
     3721 ataacgatgc cgtctgaaac cgcaaaccgc tttcagacgg cattgtcgcg tttatggaaa
     3781 aaaatgccga catcgcgtaa aatatgcgca aattttgtga ttcggtcagg tcgtctgaaa
     3841 cagattgcgc ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa
     3901 ccgagccgca gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca
     3961 tccgccaaca gcgcaacgcc tatcccaacg acttcaaacg cgacagcttc gccgccgatt
     4021 tgcacgccca atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca
     4081 aagtggccgg ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc
     4141 aagacgtgtc cgggcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt
     4201 tggacgactt caaccattgg gatttgggcg acatcgtcgg cgcggaaggc actttgttca
     4261 aaaccaacca cggcgaactg accgtacgcg tgtccgacat ccgcctgttg tccaaatcct
     4321 tgcgtccgct gccggacaaa cacaaaggct tgagcgatca ggaaaccaaa taccgccaac
     4381 gctatgttga tttgattgcc aacggagaat cgcgcaatac cttcatcaaa cgcagccaaa
     4441 tcatccaatc cgtgcgtaat tttatggtgg gcgagcatta tctcgaagtc gaaaccccga
     4501 tgatgcaccc gattcccggc ggcgcgacgg caaaaccctt cgttacccac cacaatgcct
     4561 tggacattcc gctctacctg cgcatcgccc ccgagctgta tctgaaacgc ttggttgtcg
     4621 gtggtttgga acgcgtgttt gaaatcaacc gcagcttccg caacgaaggt atgtctgtgc
     4681 gccacaatcc cgaatttacg atgattgaat tttacgaagc cttctccgac tacgaacgca
     4741 tgatgcaaat ggcggaagac atcatccgca acgcatcgcg cacggtaaac ggcacggcaa
     4801 aaatcagcta caacggcaaa gaagtcgatt tggaaagccc gtttgaacgc ctgaccattc
     4861 tcgaagccat caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat
     4921 ggctgaaaaa agaaatcgtc aaacacggcg aaagcctgcc gccgtccccg ggcatcggca
     4981 gcctgcaact cgcgctgttt gaaggttgcg ccgaaggcaa actgtggaac ccgaccttca
     5041 tcgtcgatta tccggtcgaa gtttccccgt tggcacgtgc ttccgatacc aaacaaggtc
     5101 tgaccgaacg attcgagctt ttcgttgtcg gccgcgaact ggcaaacggc tattccgagt
     5161 tgaacgaccc cgaagaccaa gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg
     5221 gcgacgacga agccatgcac tacgatgccg actacatccg cgcgatggaa ttcggtctgc
     5281 cgccgacagg cggctgcggc atcggtatcg accgcttagt gatgctgctg accgattcgc
     5341 aaaccatccg cgatgtgatt ctgtttccgc agatgcgtcc cgagtaatca taaaaacagt
     5401 tgaaatagaa atgccgtctg aacccgattg gattcagacg gcattttgta tggcaggatt
     5461 ga
//
LOCUS       DAWGJV010000130         5456 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWGJV010000130 DAWGJV010000000
VERSION     DAWGJV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.130"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WHQ94_001695"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WHQ94_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ2128938.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1075..1794)
                     /locus_tag="WHQ94_001696"
     CDS             complement(1075..1794)
                     /locus_tag="WHQ94_001696"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ2128939.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2013..2867)
                     /locus_tag="WHQ94_001697"
     CDS             complement(2013..2867)
                     /locus_tag="WHQ94_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ2128940.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFGKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2996..3724)
                     /gene="rpsB"
                     /locus_tag="WHQ94_001698"
     CDS             complement(2996..3724)
                     /gene="rpsB"
                     /locus_tag="WHQ94_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ2128941.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3901..4134)
                     /locus_tag="WHQ94_001699"
     CDS             complement(3901..4134)
                     /locus_tag="WHQ94_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128942.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4212..4793
                     /locus_tag="WHQ94_001700"
     CDS             4212..4793
                     /locus_tag="WHQ94_001700"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ2128943.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            4854..5444
                     /locus_tag="WHQ94_001701"
     CDS             4854..5444
                     /locus_tag="WHQ94_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2128944.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1215 a         1245 c         1524 g         1472 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctc ctacttccgc taccgtctga
      481 ttgggtgcgc gagcgtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa tttaaaccag
      961 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa tctccatata
     1021 ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg tcaatcagca
     1081 gtgaaccagc gtaccttcgt tttcaccggt tactacacgt ttgagcgagc cttctttagc
     1141 aatgccgaat actacaatat tgagcttgcg ttcgcggcag agggcgaaag cggtggcatc
     1201 catcactttg aggtttttca acaaggcttc gtcaaaagta atggtttcgt agcgcgtggc
     1261 ggatgagtct tttttggggt ctgcggtgta cacgccgtca acgttggtgg ctttgagcat
     1321 cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg tgaagaacgg
     1381 gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt ggatggcttt
     1441 aggacgggcg taagtttcag cgatttgctg catagacagt gcggattgta cgcgcgcttt
     1501 gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg tcgccatcat
     1561 gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata cgccccggaa
     1621 aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga cttcggcaat
     1681 ttcgccgaca gtttgaacga tggtatcgtg attgatgccg aacggatcgg aacccatcag
     1741 ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg tcatgggata
     1801 ccttgctttc ttgaatgtcg tcggtcggag tttgggccgt ctgaatttca aacggtcgga
     1861 atattaatga tagggtgtgt cggtttatta ttgttttgtt ttcagacggc ctttatattc
     1921 ggtcgtctta aaataggtac cgtaaacggt tttctcaaaa aaaagcaccc tgattcgttt
     1981 ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggctgc ggctacttcg
     2041 gctgcgtagt cgacaacggc tttttcgatg ccatcgccta ctttgtagcg tacgaagctg
     2101 accacttcag tgccgttttc tttggcgaat tgggcaacag tttgatcagg gttcatcacg
     2161 aatgcttggc cgttcagagt gatttcagcc aagaatttgc ggatacggcc ttcaaccatt
     2221 ttagcggcga tgtcggcagg tttgccggaa gcgatggctt gttcggtgta gatgtggcgt
     2281 tctttttcaa cggtttcagc atctacttcg gcttcgctta cgcattgtgg tttggcggca
     2341 acaatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc aaccaatacg
     2401 ccttcggtcg ccaatgcgcc gtggatgtag gcaaccagtt ggttggcagt gtcgatcact
     2461 tggaagcgac ggacagacat gttctcgccc aatttggcga tgatggcttt gcgttctgct
     2521 tcaaccagtt cgctcagttc ttcaacagaa gccggttttt tctcggcagc agttttcgca
     2581 acgaagttgg caaattctac gaagcccgcg tctttagcaa cgaagtcggt ttcgcagttt
     2641 acttcaacca atgcgccgac attgccgttg attgcataag ccaatacgcc ttcggcagcg
     2701 gtacggccgg ccagtttacc ggctttcgca ccggatttga tacgcaggat ttcttcggct
     2761 ttgccgaagt tgccttcggc ttcaaccaag gcttttttgc attccatcat gcccaggccg
     2821 gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt gaatctccta
     2881 gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc gcgaacggac
     2941 gttcagacgg catatttgag aaaaggggca taacgcccct cttcggtttg ccagattact
     3001 cggcagcggc ttcttgggca gcggctacgg tttcttgcag cgcttggttt ttgccttcca
     3061 aaactgcgtc agcgatgccg cggcagtaca ggcggatggc tttggcggag tcgtcgttgc
     3121 cggggatgac gtatttcacg ccgtcagggc tgttgttggt atcgactacg gcgataacgg
     3181 ggatgcccag tttttcagcc tcgaccagag tacctttttg gtagccggta tcgataacga
     3241 aaatcgcgtc gggcaggcct ttcatgtttt tgataccgcc caaagaacgt tccagttttt
     3301 caacatcgcg ttgcatttcc agaatttctt ttttgctgaa accgctttcg gcagcatttt
     3361 ccaaggctgc ggttttttct tccaggcgtt tgatggattg cttaacggtt ttgtagttgg
     3421 tcagcatacc gcccaaccag cggtaatcaa cgaaaggcat accggcgcgg gtcgcttctt
     3481 cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta cctttgttgg
     3541 caaccaaacg acgtatggct tcttgcgcgt cttggaacat cggcagggtt ttttccaggt
     3601 tgacgatatg gattttgttg cgcgcaccga aaatgtattg tgccattttc gggttccaga
     3661 aacgggtttg gtggccgaag tggacaccgg cttcaatcat ctgacgcata gtaatttgag
     3721 acattttgat ttccttgaaa gggttaaggg cacacattca atcgcataga acttgatagg
     3781 cgacgcctat tgtcaagcac ccttcggcga aagtgggcat aagttttaaa aaataaaaga
     3841 gtgtttccga aatggaaaac tgacggatta tagcggataa gaggccgtct gaaaagggat
     3901 tcagggtttt gtgccgctgt ctttttgccg ttgtgcacgc tgctgttcga attctgcctg
     3961 ttcctgttgc ttcatcaagc ggttgcggcg gcggacggtt acgacgaaga ccgtgaacac
     4021 ggtgggcagc accgcccaaa aaaccaaata aatcaaggcg cgcgcaatac tcggctgcgc
     4081 ggcggaatac ataacggcaa caaaaagata gccgatggcg acgatgtgcc acattgtgtg
     4141 cgtcctgttt gaatgttaac aaaatcgtta taatcgcaac attctaccgc aatcctgaaa
     4201 ggccgtctga aatgaggaat gaggaaaaac gcgccctgcg ccgcgaattg cgcgggcggc
     4261 gttcgcaaat ggggcgggac gtgcgggcgg cggcaacggt aaaaattaac cgcctgctca
     4321 aacgttatat caagcacggt cggaaaatcg gcgtgtattg gccgatgggc aaggaattgc
     4381 gtttggacgg ctttgtccgc gcggcgcaaa aacgcggcgc aaaactttat ctgccttata
     4441 tcgaaccgcg ttcgcggcgg atgtggttta cgccgtatcc tgaaagcgga atggaacggg
     4501 agcgcatacg gggcagggcg aggttgaacg tgccgcagtt tgcagggcgc aaaatccgcg
     4561 tgcacggttt gtcggtattg ctcgttccgc ttgtcggcat agaccgcgaa ggctaccgtt
     4621 tggggcaggc aggcggctat tacgatgcga cgcttgcagc gatgaaatac cgtttgcagg
     4681 caaaaaccgt gggcgtgggc tttgcctgcc agttggtgga cagactgtcg gtcgaggcgc
     4741 acgacctgcc gctggacggt tttgtgtcgg aggcggggat attgtgtttt tagccgttca
     4801 gacggcattt tgaacggaaa tgccgtctga aggtggaata ttgggagaaa aggatgtcgg
     4861 aaaagataat actgccggtc ttgtcgttgg gtggcgtacg gctcgaaccg ttggatgtgc
     4921 atcatgaaac gggtttgcgc gaggcggttt gcgatgggga agtttggaag ctgggagtaa
     4981 cgtccgcgcc ccatccggat agggtggcag actatatcgg gacagcattg gcaacgcgtt
     5041 tggcgtttgc ggttgtcgat gaagaggcgg gcagggtggt cgggacaacg gcgtattatc
     5101 attttgaacc gcagattccg cgtttggata ttggattcac atggtatgcg gcatcggcac
     5161 ggcggacacg gataaacacc tgttgcaaaa tcatgctgtt ggattacgca tttgatgttt
     5221 tggcttgccg ttgcgtggga tggcgcacgg atattctgaa ccttgcctcg caacgtgcca
     5281 tcgaacggct gggtgcggaa aaagacggcg tgctgcgtat gcatatgctc cgtaaagacg
     5341 gcagtgtgcg cgatacggtc gtgtacagta tgctgaggga agattggtgc aaaaacaggg
     5401 aaatcctgac cggcaggttg gcagggtatg gcgtgcaggt gtgaggcagt attttc
//
LOCUS       DAWGJV010000131         5337 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWGJV010000131 DAWGJV010000000
VERSION     DAWGJV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.131"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(104..2938)
                     /gene="lbpA"
                     /locus_tag="WHQ94_001702"
                     /gene_synonym="tbpA"
     CDS             complement(104..2938)
                     /gene="lbpA"
                     /locus_tag="WHQ94_001702"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ2128945.1"
                     /translation="MNKKHGFQLTLTALAIATAFPAYAAQAGGAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIQNPNKKAEYFLAEGEREP
                     KPVAKLAGNGNYLKNQLNRWVEERRSKGQALNSEEEAMVREAQARHENLSAQSYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SDKSGVYDGNDFRDGLYFRPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKTVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAVSRPEQLSHNAARISEST
                     GFDDNNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2935..5157)
                     /locus_tag="WHQ94_001703"
     CDS             complement(2935..5157)
                     /locus_tag="WHQ94_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ2128946.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNTAFHREDGTAIPDSKQAEEKLSFQEG
                     DVLFLYGSKENKLQQLKSEIHKRNPNVRIRTSENENKKYDYKFVDAGYVYVKGKDEIK
                     WTLDYKQFSNRLGYDGFVYYSGERPSQSLPGAGTVEYSGNWQYMTDVKRHRAGKAVGI
                     DNLGYITFYGNDVGATSYAAKDADDREKHPAKYTVDFGNKTLKGELIKNQYVKPREQQ
                     KPLTIYDITAKLDGNRFTGSAKVNTELKKSYADKEHLFFHTDADQRLEGGFFGDNGEE
                     LAGRFISNDNSVFGVFAGKQKTDASGTNPAMPSEKHTKILDSLKISVDEASDNNPRPF
                     AISPMPDLGHPDKLLVEGREIPLVNQEQIINLADGRKMTVRACCDFLTYVKLGRIKTD
                     RPASKPKAEDKGKDEEDTGVDNGEEGTEDEAAEGSEGGEDEIGDEGGGAEDEAAENEG
                     SEEDEGEEAEEPEEESPEEGGGGGSDGIPPAPEAPKGRDIDLFLKGIRTAETDIPKTG
                     TAHYTGTWEARIGKPIQWDNQADKTAKAKFTVDFGGKSISGTLTEKNGVEAAFYIEKG
                     VIEGNGFHATARTRDNGINLSGNDSTNPKTFQASDLRVEGGFYGPQAEELGGIIFNND
                     GKSLGITEGTENKVDVDAEVDAEVDAEVDVDVEQLKPEAKPQFGVVFGAKKDNKEVEK
                     "
BASE COUNT         1143 a         1435 c         1230 g         1529 t
ORIGIN      
        1 cggaaatctt gtttatattg aatcaaaaaa acctgcacct taatcagttg gcggtttagt
       61 ccgacttttg gggtgcagat caagctttca gacggtattt cctttaaaac ttcatttcga
      121 gcgcgaggct gaagtttctg cccggtgcgg cataccttcc atagttgctg tcgccgccgt
      181 gccggtttgc cgtgctttcc gcagtctggc gcaaggattc ccaagtaacg tagcggtagt
      241 tgccgatatt gtagatagcc gccctcaagg tcagccgttt tttcagattc agataggcgg
      301 aaacgtctgc cgtcgaccaa gaagacgacg ctctttttgt cgaatatcgt ttttgatcgc
      361 ctgccagata agcaagctcg tcagggtttt tccctttgga ataggtcagc ataatgtttg
      421 cgccccattt cccctctggc tggtcgtatc cgaaccccaa aacataacgc gacggctgta
      481 ccgcatccaa agcatagctg cggagggaca gtcccggccg gttggatacc gatttcggtt
      541 tgatacggtt gtacgccaat gtggtgtaca aaccttcggg cagtttgccg tacacaccgt
      601 tccagtcgat ttttcccaat atattaacgc cttggagcga catattttgg gcattgtaat
      661 aatcgcgtat atcaatctct gtcaattgtc ctgcctgatt cggcaatttg gttttgtgat
      721 cggcaacggc aatcatatcg gtatagcggt tgcggaagct gctgatttcc aaaaagccga
      781 aatcgccctt ccactgcaaa ccgatttcgc ggttggctgc cttttccgat ttcagggcgg
      841 gacgctgcca gcctttcgga taatcgtgat aaatgtctat cccgaaaagt tcttggaatg
      901 agggcgttct gaagccgctg gaggcgcggt aagacacgga aaaatgccgg ttcggtttga
      961 acacgatgcc gctgttccac gaacggtcaa cataccgtcc gctgcggacg agttcttccg
     1021 acgtggtgaa gtttttccgg tcatacctgc cgcccaagct gaaatcgaaa tatttgccga
     1081 ttgaaaaacg gtcgttcaaa gaaatgtgga tattgctgcc gttgattttt cttggcacgc
     1141 atttgcggga acgcagggtt tcgatgtagc cgcagaccga tccttcgacg acttcgggct
     1201 tacccaaaag atacttatct tgattgttat catcgaatcc cgtggattcc gaaatccttg
     1261 ccgcattgtg agaaagctgt tctgggcggg aaactgcttt ggaagcatcg taaccgaagc
     1321 ccaaagtcag atggtgtttc gtccatttgt ttttcagcga tttctcaaac gaggcattca
     1381 aaacattgtg ctgttcgcgg tagtggaaac ggtcgctgct gtcgtaggaa tacggtttgt
     1441 ccgccgacgc gcggcaggat ttgtccacgg caggatacac ggcgcaattc agcttcagcg
     1501 tgttgttgtc ggttgccacg ccctgtttgt caaacgacaa caccgtcttg tccgcccagt
     1561 tgtcagaata cgcttcgttt tcataacggt acagcaaacc catacggcgg cggcggtgat
     1621 gttcgtcaat aaatttggtg cgggaatatt tcaaacctat gcccttgacc aaatttgtat
     1681 cgcccttcca ctcttctata ttcggcctaa aatacaaacc gtcgcggaaa tcgttgccgt
     1741 cgtacacccc gctcttgtcg ctaaactttt tcgcctcatc cgtaccgtaa tactgttttt
     1801 ccgtcatatc gcggatatcg taacgctgtt tggtatcctc aaacacgccg ccgacataat
     1861 gcctgccgcc gaagcggtag cccagcttgg caagccaaga gccgctgcgg taatccatcg
     1921 gatcgggcaa tatcctgccg ccgcccgtgt acgattgggc ggacaggttt tcgtggcgcg
     1981 cctgcgcctc ccgcaccatc gcttcttctt cactgtttaa agcctgacct tttgaacggc
     2041 gttcttccac ccagcggttg agctggtttt tcaaatagtt tccgttgccc gctaattttg
     2101 ccacgggctt gggttcacgc tcgccctccg ccaagaaata ttccgccttt ttattcggat
     2161 tctgaatatc gtatgtctgg cggaacgcgt ccaaacggtc tatgccgtat tccaccccgt
     2221 ctgcaatatc gccgtgcggg cgcgtttccc gcccttggcg ttcggttcgg attagcagcc
     2281 cttcccaacc gtctttgctg aaccccgcgc cgagcgactt cataaattgg cggtttttac
     2341 tgccgtaggc ggtttttgcc tgtatccccc agcttttgcc gtctgaaatc aggtctgccg
     2401 cttctttggt gcggaaggcg accgcgccgc cgagtgcgcc gctgccgtga tcggacgaac
     2461 cggcaccttt gtcgatttcc accgtgctga tgttttcata ttcgatttcg ttgattgcac
     2521 cgctgccgcc gcgtccgccg tatccgctca acgatccttg cacggtaaac gcctgtattt
     2581 gggcaacgcc gtcaaccgaa accgccacac ggtttttatc cacgccgcgt atcgagtagc
     2641 cgccgctcgc gccgttgccc tgttcgacga ccgccacgcc cggatcgtag cgcgtcaggt
     2701 cgcggatacc gagtacctgt tctttgttca acgtttccga cgttttgacg attttgccca
     2761 aacctgtcgc ctctttcgat cgccgtccca ctttggcggc acggacggta acctctttca
     2821 atgattgagt ctgcgcggca tcaggtgccg ccccccccgc ttgggcggca taagccggaa
     2881 aagcggttgc aatggccaag gcggtcaggg taagctgaaa accgtgtttc ttattcattt
     2941 ttccacctct ttattatctt tcttcgcacc gaataccacg ccgaattggg gtttagcttc
     3001 aggttttaac tgttcaacat caacatcaac ttcagcatca acttcagcat caacttcagc
     3061 atcaacatca actttatttt cagtaccttc agttatacca agagatttcc catcattatt
     3121 gaaaataata ccgcccaatt cctccgcctg cgggccgtaa aatcctcctt ctacacgaag
     3181 atcactagct tggaaggttt tggggttggt cgaatcattt cccgaaagat tgatgccgtt
     3241 atcccgagtg cgcgctgttg cgtggaaacc gttgccctca atcacacctt tttcaatata
     3301 gaaagcagct tctacaccgt ttttctccgt cagcgttccg gaaatcgatt tcccgccgaa
     3361 atcaacggta aattttgctt ttgcggtttt atccgcctga ttgtcccatt gaatgggttt
     3421 gccgatacgc gcttcccaag tgcccgtata atgcgccgtt ccggtttttg gaatgtcggt
     3481 ttccgccgtg cggatacctt tcaggaaaag gtcgatgtcc ctgcctttag gggcttccgg
     3541 agcgggtggg atgccgtctg aaccgccacc gccgccttct tctggcgatt cttcttcggg
     3601 ttcttcagct tcttcacctt catcttcttc gctgccttcg ttttctgcgg cttcgtcttc
     3661 cgcacctcct ccttcatcgc cgatttcgtc ttcgcctcct tcgctgcctt ctgcggcttc
     3721 atcttccgtg ccttcttcgc cattatcaac gcctgtatcc tcttcatcct tccctttatc
     3781 ttccgccttt ggtttacttg ccgggcggtc ggtttttatc cgtccaagtt tcacataggt
     3841 caaaaagtcg caacaagcac ggacggtcat tttcctgccg tcggcaagat tgatgatttg
     3901 ttcttggttt accaaaggaa tttcacgccc ttcgacaaga agtttgtcgg gatgaccaag
     3961 atcgggcata ggggaaatag caaacgggcg gggattgtta tcacttgcct cgtcaacgga
     4021 aatttttaga gaatccaaga ttttggtgtg tttttcagac ggcatagcag gatttgtgcc
     4081 tgatgcgtct gttttttgtt tgcctgcgaa tacgccgaat acgctgttgt cgttactgat
     4141 aaaccgtccg gcaagctctt ctccgttatc gccgaaaaaa ccgccctcaa gccgctgatc
     4201 ggcatcggta tggaaaaaca aatgctcttt atcagcgtag ctcttcttca actcggtgtt
     4261 aactttggca ctgccggtaa agcggttgcc gtccaatttt gcagtaatgt cgtaaatggt
     4321 cagtggtttt tgctgctcac ggggtttgac atattggttt ttaatcagct cgcccttcag
     4381 ggttttgtta ccgaaatcta ccgtatattt ggcaggatgt ttttccctgt cgtcggcatc
     4441 cttagccgca taagaagttg caccaacatc gttaccataa aatgtgatat aacccaaatt
     4501 gtcaatgcca accgccttac ctgctcgatg acgtttgaca tcggtcatat attgccagtt
     4561 accggaatat tccaccgttc ccgcacccgg taaagattgg gaaggacgtt ctccggaata
     4621 atatacaaaa ccgtcataac ctaagcggtt ggaaaactgc ttgtaatcta aagtccactt
     4681 aatttcatct tttcccttta catatacata acctgcatct acaaatttat aatcatattt
     4741 tttattttca ttttctgatg tcctaattct tacattagga ttacgtttat gaatttcgct
     4801 tttaagttgt tgaagtttat tttcttttga accgtataaa aacagaacat caccttcttg
     4861 aaacgacagc ttttcttctg cttgtttgct atcgggaatt gccgtgccat cttcacgatg
     4921 aaaagcagta ttccgcctca acagccgcat tgccgcaccg acggcgggcc ggttgaccgg
     4981 cgtggtttct accgaaggcc cggcaggggg cgaagtggga acgtccttag acttgaaagt
     5041 gacggggtac gcggtcggcg ttgattcgac aacaggctgc acgccgaaat tgccgccgat
     5101 gcaagatgcc aaaagtaagg gcaacaagac aatgccgcca taattcggtt tacacatccc
     5161 tacttttcct cctatttgat taataataat tatcattata ttaatatgta cagataatat
     5221 caacccgttt ttatagtgaa ttaacaaaaa tccggacaag gcgacgagcc gcagacagta
     5281 cagatacatc ccgtcattcc cacgaaccta cattccgtca ttcccacgaa cctacat
//
LOCUS       DAWGJV010000132         5322 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWGJV010000132 DAWGJV010000000
VERSION     DAWGJV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.132"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            296..1729
                     /locus_tag="WHQ94_001704"
     CDS             296..1729
                     /locus_tag="WHQ94_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ2128947.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1838..2626
                     /locus_tag="WHQ94_001705"
     CDS             1838..2626
                     /locus_tag="WHQ94_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ2128948.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2994..4550
                     /locus_tag="WHQ94_001706"
     CDS             2994..4550
                     /locus_tag="WHQ94_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ2128949.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGDFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4567..5313
                     /gene="surE"
                     /locus_tag="WHQ94_001707"
     CDS             4567..5313
                     /gene="surE"
                     /locus_tag="WHQ94_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ2128950.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKTPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1244 a         1343 c         1515 g         1220 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttaatata
       61 ttattaaagg gctgcattac ttttctttct aaattaccat tgtataaaaa gtatttttct
      121 taaacgacag ggattgcgcc cgcgccatat tcagacaccg cccggatgtt gcgctgcccg
      181 atcggatgct tcagacggca tcggaagggt ttgcagtttt ggaatatgaa gatgataatg
      241 tccgcgagat tgacggcatt tgaaagcggt tattccgcat accggaggac acgaaatgaa
      301 cgaatattac caattaatca agcatcccga tatttccctt tccccgattt cagacggcat
      361 cggggtcggc aatccggcaa cgggcgaaat tttggcgtat gtccgcaata cgggttcgga
      421 caaactgaaa aacctgattc aaaaggcggc ggcagcgcaa aaattatggg cggcgaaaac
      481 tgctttggaa cgcgccgaca tattgtggcg ttggtatttt ttagttaaag aaaacaaaga
      541 agcactggcg cgcctgatga cgatggagca gggcaaaagc ctgaccgagt cgcgcggcga
      601 aattgattat gcggcttcgt ttgtgcgctg gtttgccgaa gaggcgcggc ggattgacgg
      661 cgatgtgtta acgagtgtaa aagcgtcgca aaaactggtt gtgttgaaac agcctgtcgg
      721 cgttaccgcc gcgattacgc cgtggaactt cccatctgca atgattgcgc gcaaggctgc
      781 gcctgctttg gcggtgggtt gcgcgatgat catcaaaccc gcatcgctca cgcctttgag
      841 tgcgtatgcc ttggcttcgt tggcttacga agcgggcata ccgcaggatt tgttgcccgt
      901 cgtcagcggc cgcgcatcgg aaatcggccg tgaatttgcc acgaacccga ttatccgcaa
      961 aatcagcttc accggttcga ccgaagtcgg cgcgaaaatt tttgccgaca gcgcggcgga
     1021 cattaaaaaa ctcagtttgg aactgggcgg caacgcgccg tttatcgtgt ttgacgatgc
     1081 cgatttggac aaagccgtcg aaggtgcgct cgccagcaag ttccgcaaca gcggtcagac
     1141 ctgcgtctgc accaaccgcg tttacgccca atccggcatt tacgacgaat tttgtcgcaa
     1201 attgagtgaa aaagtagccg agctcaaatt gggcaacggc ttggaggatg gcgtcaacca
     1261 agggccgctg attgaggaaa aagcggtaga aaaagtcgag cagcacattg ctgacgcgct
     1321 cgctaaaggc gcggtctgtc tgaccggcgg caaacgcagc gcattgggcg gaacgttttt
     1381 tgagccgacc gtcttaagcg gcgtaacggc gcaaatggcg gtggctcgcg aagaaacctt
     1441 cgggcctttg tgtccggtat tccgttttga aaccgaagcc gaagtcattg aggctgcgaa
     1501 caatacggaa tacggtttgg ctgcttacct ttttaccgcc gacaccgcac gccagtggcg
     1561 cgtcggcgaa gcattggaat acggtatggt cggcatcaat acgggcttaa tcagtaatga
     1621 agcggcaccg ttcggcggcg tgaaacgcag cggtttggga cgtgaaggca gcaaatacgg
     1681 tgcggacgaa tatctggaat tgaaatatct gtgtatggat gtcgggtgac ggatgccgtc
     1741 tgaacggcag ggtttcagac ggcgccgcat tttaagcagt ctctatctgt tgtacaatgc
     1801 gccctgtttt tacggttatt tttgatttga acaagatatg atggaaaacg gaaaaacagt
     1861 cccgagatgg cgttttgcct tgaaaagtgc gggctggcac ctcttaatca gcctgtcggt
     1921 tgcagggctg gcggcattgc tggtttttaa ggtttggtat ccttatcctt atgccgagct
     1981 gacgggaggg ctgtcgcttt atcagctggt ggtggctgtc gatattgtat gtggtccgct
     2041 gctgacttta attttggcaa gcccgaagaa aaagacaaag gcacgcatgg tcgatttttc
     2101 catggtcggc atcatccagc tggcggcttt ggtgtacggt ctgcacagcg tttctttggc
     2161 gcgccctgtg atagaggcgt ttgaaaaaga caggattaat gttgtgaccg ccgccgaagt
     2221 ggcggttgaa gatttgcaca aagcgccgga agggctgcaa agcctgtcgt ggttcggcat
     2281 ccgccgcatt gcattgaaag aacctgagga tgcggatgag aagaacaaga cgctggattt
     2341 gtccctgaaa ggtatcgagc cgagtatgcg tcccgaccgg tggctgccgt attccgacaa
     2401 ggaagcagaa aaaatccgca aacatctgaa accgctgaaa gtcttggcgg atgcgagaaa
     2461 aacgacggtt gcggacattc tgaaacaggc aggtctcgcc gaaggggagg agctgtatta
     2521 cctgccgttt accagcagca ggcagaaaga gtggatagtc attaccgata aagagggcaa
     2581 caccaaaggc tacgcgccga tagacggctt catcatcacc ccttaagcgt tgggactccg
     2641 tccgcactcg aacatccgtt cttcgcggcg gtagaatcag actgtatttg agaggggcgc
     2701 aacccctcaa tcagggcaac acatagggga aggctttatg tgccgtccgt gtgttgaaac
     2761 atttcaaaat aagctcatgc cgtccgaaca tcctttcaga cggtatggcg ttttgccatt
     2821 gccccgataa gctgttaaac tatttaaatt atttcgaccg aaggtacacg cacatgcaag
     2881 accccgagca gagcagcaag cccgcccgcc gttttttgtg cgtaccgtct gttcaggcag
     2941 cttgagcgct tcaggctgcc ttttcgcgtt tgaacttgag gaatacgaaa atcatggatt
     3001 tcagttggtt ggcagaaccg catacctgga taggttttgc cacgcttttg gtgttggaag
     3061 tcgtattggg gatagacaat cttgtctttg tggcgatttt ggcaaacaag gtccagcccg
     3121 cacggcgcga ccgcgcacgg attatcgggc tggggctggc agtcgtcatc cgcatcatta
     3181 tgcttgcttt tatggcgcac atcatcacgc tgaccgagcc gctgttccaa atcggcggcc
     3241 tcgccgtttc cggcaaggac atgattatgc tcgcgggcgg tattttcctg ctttacaaag
     3301 ccaccaccga actgcatgaa cgcctcgaag ggcacaaccg ttttactgtt gccgacaacc
     3361 aaaaaaaaca cgcgccgttt tggggcgtgg tcgcgcaaat cctgatactg gatgccgtgt
     3421 tttccatcga ttcggtcatt actgcggtgg cgatggtcga tcatatcgtc gtggcgatgg
     3481 gtgcggtcgt cgtcgcaatg gctgtaatga tttctgccag caaactcttg accgaatttg
     3541 tcgacagaca ccctaccgtc gtgatgctct gccttggttt tttgttgatg atcggtttca
     3601 gcctgattgc cgaagccttc catttccaca ttcccaaagg ctacctctac gccgccatcg
     3661 gcttctcgat tttaatcgaa ctgtttaacc agatttccca gcgcaacagc cgcaaaaacg
     3721 actacatcgg cagctcgtgg cgcaaacgga ctgctgaaaa tgtcttgggt atgatgggca
     3781 tacgcgaaag cgtgcttgcc gacgcgggcg gcgaatccgg ggacgacgcg cattttgaag
     3841 aaaacgaaaa atcgatgata cgcagcgtgc tgacgcttgc cgaacgcccg attatggggg
     3901 tgatgatccc acgccgcgac atcgaacggc tggacatttc ccaaagccgc gaagaacagt
     3961 atgcccaact gcaaaacacg ccttacagcc gcctgctcgt tgtcggaaag gcgggcgtgg
     4021 acgaaccttt gggctacatc aacaaaaaag acctgctgtc ccaactgctg gaaacaggcg
     4081 gtctcgacat tcagacggca ttgcgccagc cgctcgtcct acccgacggc acgaccgcct
     4141 tgggcgcgct cgaactcttc cgccaaagca gcgcggatta cgccctcgtc gtggacgagt
     4201 tcggcgcggt gttgggtatg gtgacgatga aagacctgtt ggaagccatt gccggcgact
     4261 ttcccgaaga attcgaacgc gaagaagaac cagccgttca ggggaatccc gatgaaagcc
     4321 tgacggtgga aggcgcgttg gaatatgtgg agctggcatc ccaactcaat ctgccgcagc
     4381 aggaggaaga tgccgatttc catacggttg ccgggctgat tatggaagaa ttgcaaacca
     4441 tccccgatgt cggtgatttt gccgatttcc acggctggcg gtttgaagtg gtcgaaaaag
     4501 aagggcagcg catcgagcgg gtcaaaatca ccaaattgcc cgaagaataa gtattcagga
     4561 tagaaaatga acgttttgat ttccaacgac gacggctacc tctccgaagg catcgccgtt
     4621 ttggcgcgcg ttacggcgga atttgccaac gtcagggtgg tcgcgcccga acgcgacagg
     4681 agcggggtca gcaattcgct gacgctggaa cgccctttgc agttgaaaca ggcgcaaaac
     4741 gggttctact atgtcaacgg cacgccgacc gactgcatcc acatcgggca gtctgtattt
     4801 tcggattttc aggccgattt tgtcttttcg ggcatcaacc ggggcgcgaa tatgggggac
     4861 gacacgcttt attcggggac ggttgcggcg gcaaccgaag cctaccttat gggcataccc
     4921 gccgtggcgt tttccttaaa cgacgcttcc ggacgctatt gggcgaccgc agaacaggca
     4981 ctgtggacat tgttggcgca ttttttcaaa acccccccgc agtcccctat tttatggaac
     5041 atcaatatcc ccgccgtcgc gccggaagat gtgcggggca ttaaaatcgc ccgtttgggc
     5101 aggagacacc acggtcagaa cgtcatcccc gcgcgcaatc cgcgcggcga acagatttat
     5161 tggatagggc cggtcggcga agtttccgat cgggaagagg gaacggattt cggtgaatgc
     5221 ggcgcaggtt tcattaccgt aacgccgctg caaatcgacc tgaccgccta tccggacatg
     5281 gcggaaacag cggcgttctg gcatgcggac tgaccgtttc at
//
LOCUS       DAWGJV010000133         5286 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWGJV010000133 DAWGJV010000000
VERSION     DAWGJV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.133"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            116..811
                     /gene="pgl"
                     /locus_tag="WHQ94_001708"
     CDS             116..811
                     /gene="pgl"
                     /locus_tag="WHQ94_001708"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ2128951.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            792..1778
                     /locus_tag="WHQ94_001709"
     CDS             792..1778
                     /locus_tag="WHQ94_001709"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ2128952.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1828..2676
                     /gene="hexR"
                     /locus_tag="WHQ94_001710"
     CDS             1828..2676
                     /gene="hexR"
                     /locus_tag="WHQ94_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ2128953.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2797..4443
                     /gene="pgi"
                     /locus_tag="WHQ94_001711"
     CDS             2797..4443
                     /gene="pgi"
                     /locus_tag="WHQ94_001711"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ2128954.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4457..5008
                     /locus_tag="WHQ94_001712"
     CDS             4457..5008
                     /locus_tag="WHQ94_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ2128955.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1344 a         1474 c         1398 g         1070 t
ORIGIN      
        1 aaaaaagcag tcccgatatt tggtatcaaa agcataaaca actgttcggc cgacatattg
       61 ctcaacccgt ttccataacg gaatatgccg tctgaaataa aaaaaggaca caaatatgtt
      121 tgtttggcac gaatacgaaa atgcggcaga agcggcgcag tctttggctg acgcagtggc
      181 ggatgctttg cagggcgcac tggacgagaa gggcggtgcg gtgttggcag tttccggcgg
      241 acgttcgccg attgcatttt tcaacgccct gtcgcaaaaa gatttggatt ggaaaaacgt
      301 cggcatcacc ttggcagatg aacgcatcgt gccgaccgtc cacgccgaca gcaataccgg
      361 tttggtgcgc gaatacctgt tgaagaacaa agcggaagcg gcaatgtgga ttcctatggt
      421 ggaagacgga aaaactgaaa ccgaattaca tcccgatgct gttgtcgatt atgcactgaa
      481 acattacaaa cagcccgatg ttttggtttt gggtatggga aacgacgggc atacggcttc
      541 gattttcccg aaagctccgc agtttcagac ggcaatcgac ggttcggcag gtgtcgcgct
      601 ggtgcatacc acgcccgtta ccgcgccgca cgagcgcgtc agtatgacct tggatgcgat
      661 tgcccatacg gggcatgtgt ttttggcgat acggggcgaa gagaaaaaag ccgtgttcga
      721 ccaagccgca caaggcgaaa accgcgaata tccgatcaac ctcgttttga accatcaagg
      781 agtgaactgc catgtcttct acgccgaata aacaagccgg atatccccga ctggtcgccg
      841 atatcggcgg gacgaatgca cgctttgcgc tggaaaccgc gccgcgcgtc attgaaaaag
      901 ccgccgtgct tccgtgtaaa gactacgata cggttaccga tgcggtgcgt gcctatctga
      961 atcaaagcgg tgcaacagcc gtacggcacg cggcatttgc catcgccaac ccgattttgg
     1021 gcgactgggt gcagatgacc aaccaccatt gggcgttttc catcgaaacc acccgtcaga
     1081 ctttggggct ggacaccctc atccttttga acgactttac cgcgcaggca ttggcggtaa
     1141 cgcagacttc aagcaaagac ctgatgcagg taggcgggca aaagcctgtc gaatttgccc
     1201 ccaaagccgt tatcggcccc ggtaccggcc tgggcgtgag cggattggtg cacagccacg
     1261 caggctgggt ggctttggcg ggcgagggcg ggcataccag tttcccgccg tttgacgata
     1321 tggaagtgct gatttggcag tacgccaaaa acaaatacgg ccatgtttcc gccgaacgct
     1381 ttttgagcgg cgcgggcttg agcttggttt acgaggcttt ggctgcaaaa cagaaagcca
     1441 aacccgccaa actgatgccg tctgaaatca cggaaaaggc tttgagcggc gcgtcgcctt
     1501 tgtgccgtca gactttggac atcttctgcg ccatgctcgg cacggttgct tccaacctcg
     1561 ccctgacgct gggcgcgcgc ggcggcgtgt acctgtgcgg cggcattatt ccccgcgtgt
     1621 tggaatattt caaaacttcc ccgttccgca gccgtttcga gaacaagggc aggtttgaag
     1681 catatcttgc cgcgattccc gtgtatgtcg tcttgagcga gtttcccgga atttccggtg
     1741 cggctgcggc tctcgacaac catttgagaa acgtttaacc acagcggctc cttgcagcgg
     1801 ggctgcatta tcgaagggca tatcattatg ttaagcaaaa tcagcgaatc actggcaaac
     1861 ctttccggtg cggaacgcaa agtcgccgaa tgtgcattgg cggaacccaa atggttcgtc
     1921 catgccgccg ttgccgaaat tgccgaacgt gcgtccgtca gccagccgac cgtcatccga
     1981 ttctgccgca gcttgggtta taaagggctg cccgagttca agctcgcctt gtccgccagc
     2041 atcggtcatg agggtatgcc ctatgtccac gaagaactca acgccgacga cgatatggca
     2101 agcgtggtcg agaaagtgtt gggcaatgcc gccgcctcgc tgttgggcga acgccgcttc
     2161 ctgaaagagt cggagctgga aaacgccatt gccacgctga tgcacgcccg tcgcgtcgag
     2221 ttttacggtg tcggcaattc cggcattgtg gcacaggacg cgcagcataa atttttccgt
     2281 ttcggcatgt ccaccgtcgc ctatgtcgat acgcacacgc agctgatggc ggcatctgtt
     2341 ttgagcgatc aggatgtttt ggttgccatt tccaacacgg gttcgtctat cgaacttttg
     2401 gatgcggtca gcatcgccaa agaaaacggc gcgtctgtca tcgcactgac ccgcaacgat
     2461 tcgcctctgg cgcaacttgc cgactgcgtg ttgagcgttg ccacacagga aaatgccgaa
     2521 ctctacacgc ccatggtttc ccgcctcttg cagcttgccg tcatcgacat tctcgccatc
     2581 ggacttgccc tgcgcttggg cgatgctgcc agcctgcaat tgcagaaaag caaaaaaagc
     2641 atacacaaca agcacatcga ttacgacaaa gattgacctt cagacggcat cccacaaatg
     2701 ccgtctgaaa tgccgaacaa cggtcgtcgg cggcttgcgg cagtttccgg cagccttttc
     2761 atcccacaac aaaaaacctc attcaggagc atatagatga aacaccttca cgacttaccc
     2821 gcatggtcga aattgtggaa ccactttgac gacagcaaaa cattgcatat gcgcgaaatg
     2881 ttcgagcaag acccgcagcg tgcggaacgc tactggctgc aagtcggcgg actgacgctg
     2941 gactactcca aaaaccgcat caacgacgaa accatgtcgc ttttgttcga gcttgcccga
     3001 gaagcaggcg tgccggagcg gatgcggcag atgttccacg gcgaaaaaat caataccacc
     3061 gaaaaccgcg ccgtcctgca tgtcgccctt cgcaaccgca ccaattcgcc gattgtggtt
     3121 gacggtgaag atgtgatgcc caaagtcaac cgcgttttgc aacgtatggg cgaatttgca
     3181 cacgaagtcc gcagcggaag ctggctgggc tataccaacc aagtcattac cgacgttgtc
     3241 aacatcggca tcggcggatc ggatttgggc ccgctgatga tgtgtaccgc gctcaaacct
     3301 ttcggtcatc cgcgcctcaa tatgcacttc gtctccaacg tggacggctc gcaactgcgc
     3361 gacgtattgt ccaaagtcca ccccgaaacc acgctgttca tcatcgcctc caaaacattt
     3421 accacgcagg aaacgctgac caacgcgctg accgcgcgcg aatggttttt gaatcatgcg
     3481 ggcgacgaag aagccgttgc caaacacttc gccgccgttt ccaccaatca aaaagccgtc
     3541 gccgaattcg gcatcgacac cgccaatatg tttgaatttt gggattgggt cggcggtcgg
     3601 tacagcctgt ggtccgccat cggattgccg attatgctgt atctcggcga agaaaacttc
     3661 attgaaatgc tcaacggcgc gcacctgatg gaccaacact tcatcaacac accgctcgag
     3721 cgcaacctgc ccgtcattct cgccctcatc ggcatctggt atatcaacta ctacggcggc
     3781 ggcagccacg tcatcgcgcc ttacgaccaa catttgcacc gcctgcccaa attcatccag
     3841 cagctcgata tggaaagcaa cggcaaacag gttacgttgg acggcaaagc agtcggacac
     3901 gaaacctcgc cgattatctg gggcgaaacg ggcattaacg gccagcacgc cttcttccaa
     3961 ctgctgcacc aaggcacgca cattaccccc atcgacctga ttgcctcgct tgaaaaacgc
     4021 agcaacctgc ccggacacca cgaaatcctg cttgccaacg tcttcgccca agcagaagcc
     4081 tttatgcgcg gcaaaacccc cgacgaagtc cgtgccgaac tcaaagcgca gggtatggat
     4141 gaggcgcgca tcgaagagct ggtcccgcac aaaaccttct ccggcaaccg cccgaccaac
     4201 ctcattctca tggacaaggt caacccgcgc aatatgggca gcctgattgc catgtacgaa
     4261 cacaaaacct tcgtacaagg catcatttgg ggcatcaaca gcttcgacca gtggggcgtg
     4321 gaactcggca aacaactggc taaaaccatt ttgggcgaac tgaccggcga aaccgggccg
     4381 caaaagcacg acagttcgac cgaacgcctg atcaacctct acctgcagac caaccgcaaa
     4441 taaaacctgc ggaaaaatgc cgtctgaacg ccgaccgttt caaacggcat ttttatcgaa
     4501 caggaaaacc gtcggtaaac ttgcagagcg tgtgcaatcc cgatatgatg gtttgcataa
     4561 atttaaacat atatgttccg cagctatggc actgattaaa gagccgttgg acaaagcgaa
     4621 acaaaggaac gaagaacttg aagcggcaga agaagcggcg gcgcaggagg cattgggtcg
     4681 ggagcaggaa gtcgaccgcg tatccgaatg ggaagaacgc tacaagctgt cgcgcagcga
     4741 gttcgagcaa ttctggaaag gattgcctca aaccgtacag aataagctgc aagcctcaca
     4801 gaaaacatgg aaaagcggga tggataaaat ctgtgccaac aatgcgaaag ctgaaggtga
     4861 aacgccaaac ggcataaaat tcagcgaact ggcatgcaaa acggcggaaa ccgaagcacg
     4921 cttggaagag ctgcacaacc gtaaaaaagc ccttatcgac gaaatggcca gggaagcgga
     4981 caagaaagaa ctgccaaagc ggctctgaac agcgcggttc aggcactgcc cgccgatatt
     5041 gccgaaaccg tcatgcccga gtaccgcaac tgacaaaacg gtttgaatgc caaatgcgcc
     5101 gacggcgacg aatacggcgt ggcacaatct gactgccgta ccagagaaat caatgcgaaa
     5161 accaaagaaa tccaaggtta tctgattgac tgaaacttgg atgcgggaat gtcggcggct
     5221 tttgcgtttt tgtcgttttt atagtgaatt aaatttaaac cgatacagcg ttggctcgcc
     5281 ttagct
//
LOCUS       DAWGJV010000134         5098 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWGJV010000134 DAWGJV010000000
VERSION     DAWGJV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.134"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(7..1263)
                     /locus_tag="WHQ94_001713"
     CDS             complement(7..1263)
                     /locus_tag="WHQ94_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ2128956.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAEIAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCVELLQNLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1611..1952)
                     /locus_tag="WHQ94_001714"
     CDS             complement(1611..1952)
                     /locus_tag="WHQ94_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128957.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2156..3013)
                     /locus_tag="WHQ94_001715"
     CDS             complement(2156..3013)
                     /locus_tag="WHQ94_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ2128958.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3074..3889)
                     /locus_tag="WHQ94_001716"
     CDS             complement(3074..3889)
                     /locus_tag="WHQ94_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ2128959.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3953..4123
                     /locus_tag="WHQ94_001717"
     CDS             3953..4123
                     /locus_tag="WHQ94_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128960.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4133..4918
                     /locus_tag="WHQ94_001718"
     CDS             4133..4918
                     /locus_tag="WHQ94_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ2128961.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         1154 a         1194 c         1386 g         1364 t
ORIGIN      
        1 accggtttat ttgcgttgca atacggcggc gaagaagccg tcggtttggt gctttgccga
       61 atcgaggcgc aggtattttc cggtatccaa atcaatcttc aggttttgca gcagttcgac
      121 gcagttgacg ggttcaaatt cgggatgttc ggacaggaaa cgttcgactt gcagctcgtt
      181 ttcttcgggc aggatgctgc aagtggcgta aaccaagcgg ccttgcggtt ttaccagttt
      241 ggaggcagca tcgaggatgc tgtgttgctg ttccaaaagg ttggccacgg tctcggcaga
      301 ttggcggtat ttgaggtcgg gattgcggcg taaagtaccc aaaccggagc agggcgcgtc
      361 caccaacaca cggtcggctt tgcctgccag tcgggcgata cggctgtcgt gttcgctgct
      421 gatgcgttcg gggtggatat tggtcagtcc ggcgcgggtc atacgcggtt tgaggttggc
      481 aaggcgtttt tcggcgatgt cgaaggcgta gattctgcct ttattggcca tttgcgcacc
      541 gacagccaag gttttgccgc cggcaccggc acagaaatcg acaatgattt cgccgcgttt
      601 tgcgcccacc aataaagcaa gcagctggct gccttcgtct tgaacttcca gcgtaccgtc
      661 caaaaacagc tcgtgtttgt taagcgcgat tttgtttttc aggcggatgc cccaaggcga
      721 ataaggcgtt gcctctgcat cggcactttc ggcttgcaac aatggcagca ctttatcgcg
      781 tttgcctttc aaggtattga cgcgtatatc gagcggcgca gcttggttga tgctgcggcc
      841 gaaggcgagg atttcttctt cgcgccaatg ctgctgcagt tgttcgacca gccattgcgg
      901 caattctgcg gcggtattca ggccgtctga aaactcggtt ttacgggctt tcaaattgcc
      961 gagaaactct atttcttctt catcaagcag gtctttgatt tggctgatgt tggtgcttct
     1021 gccgagaacc agtgcggcga gtgcggcttt gcgcggctgc gcgtgcggac ggcgcagggc
     1081 ggtactgatt ttttgatagt ggcgcagcgc ggcaaaggcg atttcggcga tttcgtggcg
     1141 gtcttggcgg ccgagctttt tgtgttcgcg gaaataggcg gagaggacgg catcggaagg
     1201 ctgtttgaaa gtcagcattt cagccaaaac tttggcggta tgatcgagtt gtgcggcgtt
     1261 cataatgtaa tgggttctct tactgtgggt tattcataat caatctaacg tccttaaaat
     1321 aacaatagca tctttatcat tacttagtat tctatccgca ataagaaaga tgtgttaaaa
     1381 gtgcgcaatc gggatttgtc taaatttctc cgttaactac aaaggcagcc tgaaaaaagt
     1441 tctattttca ggtcgcttga acagcatccg atataatcac atcagcgatt tcattttgta
     1501 atcactccct aaaacatgag gaaaaaaaca aaatttattt tcttcgttca gtatatctat
     1561 cctaaatatg ccatcactcc attgagtttt taatttgtat ttcgctcttg ttaaatgttt
     1621 ttctgtttgc cgatttcttt tgctaatgta aaaaatactc taccactctc attggttaaa
     1681 gttttcaagt attcatcaac atcagtaaca ttaataacaa ctgtttcatt tgtatgttta
     1741 ttaaattcta aatcaataat aatattccca ttaaccaata tttcatatat atcttcttct
     1801 tcagggagca tagacaaaca ataagcactt gaagctgaaa actctttttt cagatactga
     1861 agaagaaaag tatttttaat taaaatatta ttaaatttat ctaatgattg cttgacttct
     1921 ttagttgtcc aatttttaac attattcttc attgtattct acctggggtc gtagttatta
     1981 aattaccata cttatttgtt tggatcgtca taatattagt tggcatgttg ttgttatatt
     2041 tatcatatcc aatatcctta ttaactcgga tgactctttc ataaattgta tgggaaatga
     2101 gatttccatc actaagcctc tcaatagata tacgcgttaa tgttgggaaa gggtttcaga
     2161 cggccttcat aatgcgttcg atttcgtgcc aaaagccgtc cggccgacat tccaaaacgg
     2221 caatcaagcc ttggtcgata cggcggattt cggttgcgtt caatgtttcg gtttcggcga
     2281 cacgcgcagg cgcgaggtag gccatacggt cgagcagatg gtagcggact tcttggcgtt
     2341 taaacccgta ttccgcccaa tcttgaatat agatgccgcg ccaaccgtat ggattaagcg
     2401 gtggctcaaa agcatggaaa ccgtgtggaa aaaatccgat gccgcgcacg atggaaacgg
     2461 gttttacgtc aagcggcaga ccgtgttgcc gcaaggttcg gatgccgtct gaagtgtgcg
     2521 gcagaccgag ttgggcggtc agtttggcgg cttttcccaa cagcgtgtct ttggggtcga
     2581 gtccgcgcat gtcttcgagc ctgcccgtgc cgccgccgta atatttgcag gtcagctcga
     2641 tatggtaggg cttgccgtta agccttacca caaaatccgc cgcgccttgc gtattgccgt
     2701 ccgaaccgga aaccgtgagg ttgtgcgcga gcagttcggc gtgtggcgcg ttggcaaacc
     2761 aaaatgccag cagttcttcg gcgtaaatgc cgaggcggtg tccgaacggg gcgcgtcggg
     2821 tgaggtgttc cgtcagcggc gcgggatcgg catccaatgc caaaagatag cggaaaccgc
     2881 gttcccccaa tagttcgcgc acgcccaatt cgcaaccgct ttgccacaaa ggcggcgcag
     2941 tcagcagcga ggcgaggtcg cggacgggtt ggctggtgag cttccaccat aatgcgtcta
     3001 gggcgtaatt catgcttatc cgaatatcag tcatgccgtc tgaaaagctg gaaatgtttt
     3061 cagacggcct taatcagtct tttttatgga tacgatggct gacggtattg aaaaaaccgc
     3121 gcaggatatc gatgtcttcg gtttgcgtgt tggcgcgtcc gaacaggctc tgcatacggc
     3181 gcatcagacg ctcgccgttg cggcggttga aaaagccgat gtcgttcatc acgctttcca
     3241 tgtgggcgac catgcctttg atttgctcgt gggtcgcagc gtggtcttct tgttgaagat
     3301 gggtcatggg cgaaccggtt tggctgaaga tttcgtagca cacgacctgc acggcttggg
     3361 cgaggttgag cgagaaatag tcgggattgc cgttgatggt catcagtcgg ttgcaggctt
     3421 ggacttcttc gatgctcaag ccgaaagtct cgttgccgaa aaccagtgcc actttctcgc
     3481 ctcggtttgc ggtctgcagt aattcggata ccaaatcgcg cggggtttgc agcggcgcag
     3541 taatttcgcg gcggcggctg gtcagggcgc aggcgatggt ggtgtcggca agggcttcgt
     3601 ccaaagaagc ggcaatggtg gcattttcca aaacgtctgc cgcgccggaa gcgaggatga
     3661 agctttcttc cggtaattta aacgattgag gatgctccgg gtcaaacacg ggcgggtttt
     3721 ccgtcatcgg cgttgccatc agatttgggg cgacgatggt cagtttgtgc agacccattg
     3781 ttttcatcgc gcgcgcggca gagccgatgt tggcgggatg gctggtgcgc gtgaggatga
     3841 tgcggatgtt gtccagataa gcgggcaggg cgggtttgag agtagtcatg ttcggttttc
     3901 ggtcggaatg gaatagggta taatgcgccg tctttttcgt tcggacggtc aaatgccgtc
     3961 tgaaatgttc tttaacaacg tcggaaattt aacgttgcgc ctgaagcaga cacgcgtcgg
     4021 gcggtatttt acccgatttg gcaggattta ccccaaccaa ataaactccg attttgccgc
     4081 tccgaaggac tgcgcgtccg aaccggcggt atgccaccct taaggaaatc tgatgaatcc
     4141 gtttttgaat acagccttta aagccgcccg ccgtgccggt cagatgatga tacgcgccgc
     4201 gggcaatctc gatgccgtca aaaccgacag caaagcattc aacgattttg tttccgatgt
     4261 tgaccgcaat tccgaaatca tcctggttga ggctttgaaa gaagcctatc cgcaccacaa
     4321 aatcacttgc gaagaaagcg gctcccacgg taaagccgca gccgaatacg aatggattat
     4381 cgatccgctc gacggcacga ccaacttcct tcacggccat ccccaatacg ccatctctat
     4441 ggcactcctg cacaaaggcg tgttgcaaga agctttggtg tacgcccccg aacgcaacga
     4501 cgtttacatg gcttcgcgcg gcaaaggcgc gttgctcaat gaccgccgca tccgcgtttc
     4561 caaccgcatc gaattgaacc gctgcctgat cggtaccggc tttcctgttg tcgatcaaag
     4621 catgatggac aagtatctgg cgattttgaa agatttcttg gcaaaaaccg ccggcggccg
     4681 tcgtgaaggc gcggcttctt tggatttgtg tgctgtggcg actggtcgtt ttgacggctt
     4741 tttcgagttc aacctcaaac cttgggatat tgccgcaggc gcattgattg ttcaggaagc
     4801 gggcggtatc gtaaccgata tgacgggtga agacggctgg ctggaaagcg gcgatattgt
     4861 tgccgccaat cccaaagtgc tggcgcaaat gttaaaaatc atttccgcac acgtttaaat
     4921 ttcagcccgc tcccgggaat gcgggaaaac gggcgggtat gccgtctgaa gtatcgcgcc
     4981 gatatttcag acggcatttt tactggcagc gggggcagga aggtggaaac ggctaaatgc
     5041 ccgaaactgc cgaagatgga aacccatatc tgattaaacg gagaaccctg tacgattt
//
LOCUS       DAWGJV010000135         5077 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWGJV010000135 DAWGJV010000000
VERSION     DAWGJV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.135"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            272..1504
                     /gene="pilG"
                     /locus_tag="WHQ94_001719"
     CDS             272..1504
                     /gene="pilG"
                     /locus_tag="WHQ94_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ2128962.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            1578..2438
                     /locus_tag="WHQ94_001720"
     CDS             1578..2438
                     /locus_tag="WHQ94_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ2128963.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLS
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            2440..3072
                     /locus_tag="WHQ94_001721"
     CDS             2440..3072
                     /locus_tag="WHQ94_001721"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ2128964.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            3065..3274
                     /locus_tag="WHQ94_001722"
     CDS             3065..3274
                     /locus_tag="WHQ94_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ2128965.1"
                     /translation="MTESRQTRLQVKCPTCQTTVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSDQTESLLEISEPDGAYR"
     gene            complement(3359..5035)
                     /gene="pilB"
                     /locus_tag="WHQ94_001723"
     CDS             complement(3359..5035)
                     /gene="pilB"
                     /locus_tag="WHQ94_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ2128966.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         1208 a         1417 c         1270 g         1182 t
ORIGIN      
        1 ttgagctaag gcgagccaac gccgtaccgg tttttgttaa ttcactatac cctccggccg
       61 gcatttgacc cgcaacaata tcctgcaaga tttattgtgt acgcataaat gccggacagc
      121 cgcctaaata aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg
      181 tcggcggttt tcatcttacc gggaacacgc cggtactgtt ttttaacgat gtttcttaca
      241 ttttattcca attactttac ggggctagaa tatggctaaa aacggaggat tttctttgtt
      301 cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tccgcctccg acaaactggt
      361 caacggcgaa gtatccgcgt ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg
      421 catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga
      481 agacatcacc gttttcaccc gccagctttc cacgatgatt aaagcgggcc tgccgctgat
      541 gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat
      601 ggaaatccga ggcgaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc
      661 gaaatatttc gaccgcttct actgcaacct ggttgcggcg ggcgaaacgg gcggcgtatt
      721 ggaaagcctg ctggacaaat tggcaattta caaagaaaaa acccaggcca tccgcaaaaa
      781 ggtaaaaacc gcgctgacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt
      841 cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga
      901 gcttccccca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg
      961 gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc
     1021 gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat
     1081 tgtccgcaaa gggacgattg cccgctgggg caggacgacg gcgacgctga ttgcggcagg
     1141 cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga
     1201 agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ttgtctatga cttcggggat
     1261 gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcttccatcg gcgaggaatc
     1321 gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa
     1381 tgcggtcggc aggctgtccg ctatgatgga gccgatcatt atcgtgattt taggcttggt
     1441 catcggcacg cttctggtcg ccatgtatct gccgctgttt aatttgggca acgtggtcgc
     1501 ctgatttgcc gcacagatcc ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa
     1561 tacattgaga acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg
     1621 cagcggtgtt cgggctgctg gtcggcagtt tcttaaacgt cgtcatttac cgcgtgccgg
     1681 tcatgatgga acgcggctgg acggtatttg ccaaagaata tttaaacctg ccgctgaccg
     1741 aagaggaaag ccgtaccttc aacctgatga aaccggattc ctgctgtccc aaatgccgcg
     1801 tgccgatacg cgcgtggcag aacatcccga ttgtcagcta cctgctcctg cgcggcaaat
     1861 gcgcctcctg ccaaaccaaa atcagcatac gctatccctt aatcgaactg ctgaccggcg
     1921 tattgttcgg gctggttgcc tggcaatacg gctggtcttg gattacattg ggcggtttga
     1981 tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa tatctgcccg
     2041 actcgatgac attatccttg atttggctgg ggctgatatt taatttggac ggcggcttcg
     2101 tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tggttcgata tggcttttat
     2161 gcgcggtata taaactgatt aaaggcgaaa tcggtatggg caacggagat ttcaaactga
     2221 ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct
     2281 cgctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct
     2341 tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc
     2401 gggcggtcaa ctggtggctg acccatccgg tgctgtaaga tgacggtatg ggtcggactg
     2461 accggcggaa tcggcagcgg caaatcggca gccgcgcaat gttttgccga tttgggcgtg
     2521 ccgcgcatcg atgcggatgc ggcggcgcac tcgctgacgg cttcagacgg catcgccctg
     2581 ccggaaatca ggcggctgtt cggcgacacc gttttcgaca cacagggttt gttgcgacgc
     2641 gacatattgc gtaaagaagt ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg
     2701 atgttgccgc tgattttctc agaaatcaaa aaacagcaag aaacctttac cgatgcggct
     2761 tacggcattg tcgaaattcc gctgctgacg gaaaagcgtc aatttatcag cctgatacgg
     2821 cgtgtcctga ccataagcgc acctgtggaa aaacgtatcg gcagggtgat ggcccgcagc
     2881 gggctgacgc gcggcgaggt ggcggccgtc atcagccatc aggcatccga atccgaacgc
     2941 ctgctgcttg cagacgatgt gctgctcaat gacggcagcc tcaaaagcct gcgtgagaaa
     3001 acaatgcgcc tgcacgcgtt ttattcaggg attttcgcct caaaaccaac acaaggaaaa
     3061 cacaatgact gaatcgcggc aaacacgcct tcaagtcaaa tgtccgacct gtcaaacgac
     3121 agtagtatgg aaacccgaaa acgcattccg cccgttctgt tcgcaacgct gcaaactgat
     3181 cgacttgggc ggatgggcag acgggaaata tacggtttcc gaccaaacgg aaagtttgct
     3241 ggaaatatcc gaaccggacg gcgcataccg ctgaccgcac cccgccctcc ggcaaacccc
     3301 tgaaaatcaa atgccgtctg aaacaaacac gcttcagacg gcattttcat tcccaagcct
     3361 aatcgttggt atttgccgtt acctcttcca acgaggtaat gccctgcata actttcaaaa
     3421 taccggcccg gcgcaaatcc accataccct ccttataggc aacgtccaaa atatccactt
     3481 ccgtaccgtt gttcataatc acgcgctgca tttcttcgct gatgggcata acctcataca
     3541 cgcccgcacg ccctttataa ccctgccccc ggcaacggtc gcaaccgacg gcgcggtaaa
     3601 gtttccaatc ttttgcaagg tcctcatcgg tgaagccgac ttccttcaaa gcagaggcag
     3661 acgggcgttc cacttcctgt ttgcagctcg aacacagcct gcgtaaaaga cgctgcgcca
     3721 taatcaggct gaccgaactg gcaatattaa acggcgcgac acccatattc agcatacgcg
     3781 acaacgtcgc cggcgcatta ttggtgtgca gggtggaaaa caccatatgc cctgtttgtg
     3841 ccgccttaat cgcaatatcg gcagtttcca aatcacgaat ctcaccgacc ataatgatgt
     3901 ccgggtcctg acgcaggaaa gacttcaaag cagcggcaaa agtcaggccc tgcttatcat
     3961 tgacgttaac ctgattgatg cccggcaggt taatctcggc agggtcttcc gccgttgcaa
     4021 tatttaccga ctccgtattc aaaatattca aacaggtata gagcgacacc gtcttacccg
     4081 aacccgtcgg accggttacc agcaccatcc cgtagggacg gtgaatcgct tccaacaaca
     4141 attttttctg aaacggctca aaaccgagct ggtcaatgtt caaagacgcg gcatcggaat
     4201 tcaaaatccg catcacgacc ttttcgccaa acagcgtcgg caatgtgctg acacggaaat
     4261 cgacaggctt gccgcccttt tgaaaggtca gctgcatcct gccgtcctgc ggtatccgtt
     4321 tttcggaaat gtccaaacgc gacattacct taatccggga agcaagctgc ccccttaccg
     4381 caatgggcgg ctgaaccacc tcgcggagct gcccgtccac acggaaacgg atacgggcat
     4441 tgtgttcgta aaactcgaaa tggatgtccg atgccccgct gcgcaaggca tccgacaaag
     4501 tcttatggat aaacctcgga acagggccat cttctgcctc ctcgttgtcg atatacaggg
     4561 tgtggctttc ctcttcctcc tgcccctccc caagctcctg aagcagcgat gtcgaacgcg
     4621 aacccaccca atcgagcaaa cccgccaact ggtcatcctc gacaatgacc aactcaacct
     4681 caatccctgc ggcagaaacg gttttctgaa tttgcggcat ctgtgtcgga tcggaaaccg
     4741 caaaaaatac tttgtcgccc cgacggaaaa ccggcacaca gtggaactcc accatctgct
     4801 cctccgtcaa cacccccatc agcaccctgt ggcgcggata atgacgcaaa tcaagaatcg
     4861 aataactgaa caccctcgca atcaatgccg caagcgactt gggcgaaatg acaccgtctg
     4921 aaaacagcat cggcaacact tccttacccg cctgcgactc attgtagtaa tgctcggcct
     4981 gctcaacagt aaccacctgg ttttgaacca gaatcctcag caaacctacg ctcatacgac
     5041 cttatcccca aatttattca ttgttatacc tgtacag
//
LOCUS       DAWGJV010000136         5007 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWGJV010000136 DAWGJV010000000
VERSION     DAWGJV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.136"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(236..2512)
                     /locus_tag="WHQ94_001724"
     CDS             complement(236..2512)
                     /locus_tag="WHQ94_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2128967.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVGLETVTVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2934..3524
                     /locus_tag="WHQ94_001725"
     CDS             2934..3524
                     /locus_tag="WHQ94_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ2128968.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFMGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3587..4645
                     /gene="trpD"
                     /locus_tag="WHQ94_001726"
     CDS             3587..4645
                     /gene="trpD"
                     /locus_tag="WHQ94_001726"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ2128969.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4797..>5007)
                     /locus_tag="WHQ94_001727"
     CDS             complement(4797..>5007)
                     /locus_tag="WHQ94_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ2128970.1"
                     /translation="YTVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFS
                     HLRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1144 a         1158 c         1420 g         1285 t
ORIGIN      
        1 ctgaaaccga atgaattgga ttctcgcctg cgcgggaatg atgggtttct gcttttgatt
       61 tttctgcttt tgcgagaatg acggcgtgaa agcaagaatg atgaaacaaa gaaaatggga
      121 atgatggcat agtggtttgt tctttgtctt tgccatattt cctaacaaat tgattaaaaa
      181 gaaaaaaggt tttcagaatg ccgtctgaaa accttttttg tttgcctgtc cgattttaaa
      241 acttcacgtt cacgccgccg gtaaagctgc ggcccatttg cggcgtatca gagaggaagc
      301 tgctgtgggc gtaaacggat tggttgagca ggttgtcggc tttgacgtac caattccact
      361 cgccatagcg cgtattgcgg cggtagtttg cgccgaggtt gagcatatgg tgtccgggcg
      421 tgcgcgtttc gtagcgggcg agtttgtttt gggcgaacac gcggtagtag tccaaattgg
      481 catcgatacg gtcggtcagc gaggctttca ggtggaagcc gaggcgcgca gccggaacac
      541 ggggggcatt ttggtcgtcc tgtgcgatga aaggacggtt gccgtaggca tcttctctgc
      601 cgggtaggga aggcaggttt ttcagacggc ctcgtacata gtcgccggaa acgccgatgc
      661 ggtagcgcgg tgtcggtttg aagtagattt cgccttctgc gccgtagaag tccgcaccgg
      721 attggttgta gcgcacgagc ttcatttcgc tgtcgtcttc gatggatttg gggccgcgtc
      781 cgtcgtttaa ggtttgggcg taaatgtagt tgccgaagcg gttgcggtag agtgccagat
      841 tgtattgcca gcggtcgcct tcgtagccca gcgcgagttc gatattgttg gaacgctctt
      901 tgttgaggtg tttgttgccg acttcaaagg tgttggtggc gacgtgtttg ccgtgtgcgt
      961 acagctcttg cgttgacggc aggcgttcct gatgggaggc ggtcaggctg agtttgtgtt
     1021 gtggcgtgaa ataccagttg cccgaaagtg cgaatgagcg ggcggtttgg cggtgcgcgc
     1081 cgaggtcggg caggggatgg ttgtagtagt tttcccgatc aatcaatgct ttatcgtagc
     1141 ggatggaggc tttttgtttt tccacgcgta cgccgccttc aagcgtgaag ttgtcccagt
     1201 ttgcctgttc tacaccgaaa aagctgtaat gttgcacttt attgtcaagc agcatcggtt
     1261 gtttgaccgc ttcggatgtg gcagataaag cactggattt ttgttgtaaa tattgcacgc
     1321 cccagctgcc tttcagacgg cctatgggtt ggtggcgcaa ctcgatgcgg gcgttttgcg
     1381 tttggttgtt aaaaaagttt tcgaccgcat cgcctgcttt ttcgtcgtgg cggtagtcgt
     1441 tgcggttcaa gtatacgcgc agggcttcaa aaccggggaa cggttgcttc cattcggcac
     1501 ggagttcgta gcgtttgttg cgcaggtcta tccacggtct gccgctgtgg gtgtgtgcgt
     1561 gtgcattatc gtcgtcgtgg aagccgcagc tcaagcccgg attgtcgtaa tcgatgtctt
     1621 cttcggtcaa caggtgcgga taaagctgta aatagcgttt gttaatcaag ctcttttgcc
     1681 agatgatgtc ggcgtggcaa tcatcgtatt cgtggctgtg ggcaggcaga ccatattggt
     1741 cgcgacggtc gctgtacgct acgccgataa aacctttttc gccaacccaa gacagcccga
     1801 tgctgcccgt ttgcgaatcg gcgtggctgt cgggcaggcg tttcagattg cggtaacgcg
     1861 gtacggcgta atcccccgat ttgcggtaca gcccttccgt gtgcaataca aagtttttgc
     1921 ccaaaccgat attgatgccg ccggacgtga gtttttccag attgccgctg ctcaaacgca
     1981 atccgagttc gcccgatacg ccgttttcag gcattttttc ggggattttg ccatcggcaa
     2041 catcgaccag ccccgccaca ttgcccgagc tgtacaagag cgtaaccggc ccgcgcagga
     2101 tttcgacctg ttgcgacaag gcggtatcta ccataatggc gtgatcgggc gaaaaatccg
     2161 ccatatcgcc tgtttcgccg tgatggttca acactttaat ccgcctgcct gtttgaccgc
     2221 gaataacggg agcggacgcg ccgccgccgt attgcgaggc gtgaatgccc ggcacgccgt
     2281 ccaaagcatc gcccaagttg acggcttttt gtcgcaaggt gtcgccgctg atgattttgt
     2341 cggaggcggt cgaagtgtgc agcagcccgg atgtggcgcg cggacggctt ttgccgacga
     2401 cggtaaccgt ttccaagccc accgattgct cagtttcatg cgcttgggcg aggaggggtg
     2461 tgttgattaa aagaattgat aaaacaatgg gtttgagtgt agtttgtgcc attttggctt
     2521 ctcgtcgcat ttcaaaagtt tgttattata taacattaca ttttttatat cataagattt
     2581 tgagaacact cagagggtat aggcaaaagt ttttcaaatg acacggtcgc ggcatcgggc
     2641 ggtgtccatt tgtatccgcc gtcattcggg ggagtggttg atgttgacac agattccgct
     2701 gtgtttgccc attatgttcg gctgcgggat aaccaaaatc tatgagtgca taaaaacggt
     2761 acgttcccga acggtcgaaa aggtggcaaa atggcgtact cgtcagaacg cgaggctctg
     2821 cgccagattc acgagggtgc atcgggcacg ctaagatata agagggtatg gatgggggta
     2881 tcggaaatgc agttaataac aaacaaatta taaatcaata ggttaatcac aaaatgcttt
     2941 tgtttatcga caattacgac agttttactt acaacatcgt ccagtatttc gctgaattgg
     3001 ggcaggaagt tgccgtgcgc cgcaacgatg atattacgtt ggaggaaatc gaggcattga
     3061 atccgcaata tctcgttatc ggccccggtc cgtgttcgcc caaagaagcg gggatttccg
     3121 tggcggcgat gcgccatttc gccggccggc tgccgattat gggcgtgtgc ctcgggcatc
     3181 agacgatagg cgaggcgttc ggcggcagga tagtccgcgc caaaacgctg atgcacggca
     3241 aggtgtcgcc cgtgtcccat tcgggcaagg gtatgtttat ggggttgccc aatccggtta
     3301 cctgtacgcg ttatcacagc ctcgttatcg aacgcggcac actgcccgaa tgtttggaaa
     3361 tcacggcatg gacggaagac ggcgagatta tgggcgtgcg ccataaggaa tatgccgtcg
     3421 agggcgtgca gttccacccc gaagccctct tgaccgaaca cggacatgat atgttgaaca
     3481 attttttaat cgaatttcaa aacttcaaac cgcaaaaaat ctgacgcgat gccgtctgaa
     3541 gcccttcaga cggcattttc gtccgaatat tgaacggagg acaaaaatga ttacaccgca
     3601 acaggctatc gaacgattaa tcagcaataa cgagttgttt tacgatgaaa tgaccgactt
     3661 gatgcgtcag attatgagcg gaaaagtacc gcccgaacaa attgcagcga ttttgaccgg
     3721 cttgcgtatc aaggttgaaa ccgtttccga aattaccgca gctgcagccg tgatgcgcga
     3781 gtttgcgaca aaagtgccgc tggagaatgc agaggggctg gtcgatatcg tcggtacggg
     3841 cggggatggc gcgaaaacct tcaatatttc gacgacttcg atgtttgttg ccgcagcggc
     3901 aggcgcgaag gttgccaaac acggaggccg gtcggtctct tcctccagcg gcgcggcgga
     3961 cgtggtggag cagatggggg caaacctcaa cctgactccc gaacaggttg cccaaagtat
     4021 caggcagacc ggtatagggt ttatgttcgc ccccaatcac cacagtgcca tgcgccatgt
     4081 cgcccctgta cgccgttcgc tcggtttccg aagtattttc aacatattgg gtccgttaac
     4141 gaatcctgcg ggcgcgccga accagctttt gggcgtgttc cacaccgatt tgtgcggcat
     4201 cctttccaga gtattgcaac agctcggctc caagcatgtt ttggttgttt gcggggaggg
     4261 cggtttggat gaaattacac tgacgggcaa aacacgcgtt gccgagctca aagacggaaa
     4321 aatcagcgaa tacgacatcc gcccagaaga tttcggtatc gaaacccgcc gcaatttgga
     4381 tgaaatcaaa gttgccaata ctcaggaatc tttgttgaaa atgaatgagg tgctggaagg
     4441 aagagaaggg gctgcgcgcg atatcgtatt gctcaacacc gccgccgccc tgtatgccgg
     4501 aaatgtcgct gcttcgcttt cagacggcat atctgccgca cgggaagcca tcgattcagg
     4561 cagggccaaa tcgaaaaaag aggagtttgt cggttttcaa ccacaacaaa gatgccattt
     4621 tcttggaaag atggagcttg ggtgatgccg ccatgattat ggaacttttg tggcaaaaca
     4681 taagcacttc acgaagagaa cttaccaaac tgtttttata taaaaacttg gggctgtact
     4741 agataaccag accaaattcc cattaactaa ttgtcttaaa atctgaattt gagattctat
     4801 ttaaaatgcc attggcattt ctttaaatgc agccccaaat gctctttggg aatgccgtta
     4861 aacttacgta aatggctaaa ttcactaata tcaagcacat cataactacg aaaggtatcc
     4921 gtgtatacaa tgccatcagg cttaactttc tttcggataa ttggcaacaa tgtcgctgat
     4981 tgcgcattag gaacaacgac ggtataa
//
LOCUS       DAWGJV010000137         4959 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWGJV010000137 DAWGJV010000000
VERSION     DAWGJV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.137"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            113..1018
                     /gene="glyQ"
                     /locus_tag="WHQ94_001728"
     CDS             113..1018
                     /gene="glyQ"
                     /locus_tag="WHQ94_001728"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ2128971.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1095..1505
                     /locus_tag="WHQ94_001729"
     CDS             1095..1505
                     /locus_tag="WHQ94_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ2128972.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1526..3589
                     /locus_tag="WHQ94_001730"
     CDS             1526..3589
                     /locus_tag="WHQ94_001730"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ2128973.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEYPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDNLPNGKVA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3606..4007
                     /locus_tag="WHQ94_001731"
     CDS             3606..4007
                     /locus_tag="WHQ94_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ2128974.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNAEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKWDSRIKILAHDRLLNRKQQRRDSPANTFKHR
                     LIRALTKISREREKRRQRREQLIGKIIVPFQ"
     gene            4019..4952
                     /locus_tag="WHQ94_001732"
                     /pseudo
     CDS             4019..4952
                     /locus_tag="WHQ94_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
BASE COUNT         1263 a         1399 c         1290 g         1007 t
ORIGIN      
        1 tccactatat catgtatgcc gtgttttcaa atcagtctgt ctttgagata aacctatcca
       61 aacagcaatg gaaacagtaa aataaaccct ttattttatc gaatcataag ccatgcttac
      121 cttccaacaa atcatcttca aactgcaaac attctgggca gacaaaggct gcaccgtcat
      181 ccaacccttc gacatggaag tcggtgccgg cacatcccat cccgccacct gcctgcgcgc
      241 actcggcccc gagccttggt ttgccgccta cgtccaaccc agccgccgcc ccaaagacgg
      301 ccgctacggc gacaacccca accgcctgca acactattac caattccaag tcgccctcaa
      361 gcccgctccc gccaatatcc aagacctcta tctcgactct ctgcgcgaat tgggcatcga
      421 tcccaaagtc cacgacatcc gctttgtcga agacgactgg gaaaacccca ccctcggcgc
      481 gtggggtttg ggctgggaag tctggctcaa cggcatggaa gtaacccagt ttacctattt
      541 ccaacaagtc ggcggcatcg actgcacccc cgtactcggc gaaatcacct acggcatcga
      601 acgcttggcg atgtacctgc aaggcgtgga aaacgtctat gacctcgttt gggcaaaaac
      661 gctggacggt aatacagtaa cttacggcga cgtgtaccac caaaacgaag tcgaacaatc
      721 cacctacaac ttcgaataca gcgatgccga ctggctgctg cgccagttca acgactacga
      781 agcgcaagcc aaacgcctgc ttgccgaaga aaacgccgcc ctcgccctgc ctgcctacga
      841 gcttgtcctc aaagcggggc atacgttcaa ccttttagac gcacgcggcg cgatttccgt
      901 aactgagcgg gcaacttata ttggacgtat tcgtgcattg agccgcgccg tggcgcaaaa
      961 atatgttgaa agccgcgaaa aactgggctt ccctttaatg aaggcaaacg ccgcctaagg
     1021 gggcgacgat tttttacgtt cgggcggcac gataaggcag ccgtccggaa gccattatat
     1081 tttttaggaa aatcatgcct gtctatttcc gcaccccaag caggaaacgc ctgcacaaaa
     1141 tgaaccgccg ccagcataaa aagctgcatt tgggcgagtt tcaaacattg attttcggcg
     1201 ttcaaggcag tttgcaccaa gctgaggacg atgccgaagc gttcgaccgc tttatggacg
     1261 agctgattgc gtatcttgcg gcaaacggct tggaaatggc gggcgggggc gattgcggcg
     1321 atttttcgtg cctgtttcaa aaatccggct cccgaggctt gggcgaaacc gagaaaaaag
     1381 ccattatctc ttggctggca ggtcgcgaag atgtcgccac tttgcacgcc accccgctgg
     1441 ttgacggttt ttacggggat gacggctggg aaaattacag cgaaacctat aaacaggcag
     1501 attaaccaaa ttcaaagaaa gccgaatgat gacccaaacc cttttaatcg aactccttac
     1561 cgaagaactc ccgcccaaag ctctgaataa tctgggcaac cattttgccg ccgccgttgc
     1621 cgaaggcttg gaaaaagcgc aactggttga cggtgcagcc gaatttacgg cttatgcctc
     1681 gccgcgccgt ttggcggttc aagtcaaaaa cgttaaagcc gttcaggccg accaaaaaat
     1741 cgtgaaaaaa ggccctgccg tggcgaatgc catgaaagac ggtgcgccga ccaaggcttt
     1801 ggaaggtttc gcacgcggtg cgggcgcgaa aatcgaagac ttgaccatcg tccacgacgg
     1861 caaacaggat gtgtacgcct acgaatacgt ccaaaccggc aaaccgctgg gcgaactttt
     1921 ggaagacatt atcaatcaag ccgttaagaa actgccgatt ccgaaagtga tgcgttgggg
     1981 cagcagcacg tttaccttcg tgcgccctgt tcacgggctg attgtgctgc acggcggcga
     2041 catcgtaaac gtcagcgttt tgggtctgca aagcagcaat caaaccttgg gacaccgctt
     2101 cttgtccaac ggcgaaatca ccattgaaaa cgccgacagc tacgccgaac aaatgcgcgg
     2161 gcaaggcaaa gtcgtcgctt cgtttgccga gcgcaaagcc gcgattcaga cggcattgga
     2221 agggcaggcg cgccgtctga acgcgaccgt tgccgccgat gaagcattgc tggacgaagt
     2281 aaccgcgctg gtcgaatacc ccgttgtttt ggaagccggt tttgaggaac acttcctcgc
     2341 cgtgcctcaa gaatgcttga ttttgacgat gcagcaaaac caaaaatact tcccgctgct
     2401 cgaccaaaac ggcaagctga tgaaccgctt cctgctggtc tccaacctgc aaaccgcaga
     2461 cccgtcgcac atcatccaag gcaacgaacg cgttttgcgc gcgcgcctgt ctgatgccga
     2521 gttcttctac aaacaagacc aaaaggcgac tttggaaagc cgcctgccca agctggcaaa
     2581 cgtggtttat cacaacaaaa tcggttcgca agccgaacgc atcgaacgcc tgcaaagcat
     2641 tgccgcccac atcgctaaag cgttgggcgc agatgccgcc gccgccgaac gcgccgcgcg
     2701 tctggcaaaa gccgacttgg taaccgaaat ggtcggcgag ttccctgaat tgcaaggcac
     2761 catgggcaaa tactatgccc gtttggacgg cgaaaccgaa gaaatcgccg aagccatcga
     2821 gcaacactac caaccgcgtt tcgccggcga caatctgccg aatggcaaag ttgccaccgc
     2881 cgtggcactg gcggacaaac tagaaacctt ggtcggcatt tggggcattg gtctgattcc
     2941 gaccggcgac aaagacccct acgccctgcg ccgcgccgcc ttgggtattt tgcggatgct
     3001 gatgcagtac ggtttggacg tgaacgaact gattcagacg gcattcgaca gcttccccaa
     3061 aggtttgctc aacgaaaaaa cgccgtctga aaccgccgac tttatgcagg cgcgccttgc
     3121 cgtgttgctg caaaacgatt atccgcaaga catcgttgcc gccgtattgg cgaaacagcc
     3181 gcgccgtttg gacgacgtag tggcgaaact gcaagccgtt gccgcgttca aacaactgcc
     3241 cgaagccgcc gcgctcgccg ccgccaacaa acgcgtgcaa aatctgctga aaaaagccga
     3301 tgccgagttg ggcgcggtta acgaaagcct gctgcaacag gacgaagaaa aagccctctt
     3361 tgccgccgcg caaggcttgc agccgaaaat cgccgccgcc gtcgccgaag gcaatttcca
     3421 aaccgccttg tccgaactgg cttccgtcaa gccgcaagtc gatgccttct ttgacggcgt
     3481 gatggtgatg gcggaagatg ccgccgtaaa acaaaaccgt ttgaacctgt tgaaccgttt
     3541 ggcagagcag atgaacgcgg tagccgacat cgcgcttttg ggcgagtaac cgttgtacag
     3601 tccaaatgcc gtctgaagcc ttcagacggc atcgtgccta tcgggagaat aaattgcagc
     3661 ctttagtcag cgtattgatt tgcgcctaca acgcagaaaa atatttcgcc caatcattag
     3721 ccgccgtcgt gaatcagact tggcgcaact tggagatttt gattgtcgat gacggctcga
     3781 cagacggcac acttgccatt gccaaggatt ttcaaaagtg ggacagccgt atcaaaatcc
     3841 ttgcacacga ccgcctcctg aaccgcaaac agcaaaggcg cgattccccc gccaacacat
     3901 tcaaacaccg cctgatccgc gccttgacca aaatcagcag ggaaagggaa aaacgtcggc
     3961 aaaggcgcga acagttaatc ggcaagatca ttgtgccttt ccaataaaag gagaaaagat
     4021 ggacatcgta tttgcggcag acgacaacta tgccgcctat ctttgcgttg cggcaaaaag
     4081 cgtggaagcg gcccatcccg atacggaaat caggttccac gtcctcgatg ccggcatcag
     4141 tgaggcaaac cgggcggcgg ttgccgccaa tttgcggggg ggggggtaat atccgcttta
     4201 tagacgtaaa ccccgaagat ttcgccggct tccccttaaa catcaggcac atttccatta
     4261 cgacttatgc ccgcctgaaa ttgggcgaat acattgccga ttgcgataaa gtcctgtatc
     4321 tggatataga cgtattggtc agggacagcc tgacgccctt atgggatacc gatttgggcg
     4381 ataactggct tggcgcgtgc attgatttgt ttgtcgaaag gcaggaaggc tacaaacaaa
     4441 aaatcggtat ggcggacggc gaatattatt tcaatgccgg cgtattgctg atcaacctga
     4501 aaaagtggcg gcggcacgat attttcaaaa tgtcctgcga atgggtggaa caatacaagg
     4561 acgtgatgca atatcaggat caggacattt tgaacgggct gtttaaaggc ggggtgtgtt
     4621 atgcgaacag ccgtttcaac tttatgccga ccaattatgc ctttatggcg aacaggtttg
     4681 cgtcccgcca taccgacccg ctttaccgcg accgtaccaa tacggtgatg cccgtcgccg
     4741 tcagccatta ttgcggcccg gcaaagccgt ggcacaggga ctgcaccgcg tggggtgcgg
     4801 aacgtttcac agagttggcg ggcagcctga cgaccgttcc cgaagaatgg cgcggcaaac
     4861 ttgccgtccc gcaccgtatg ttttcgacaa agcgtatgct tcaaagatgg cgcagaaagc
     4921 tgtctgccag attcttacgc aagatttatt gacggggca
//
LOCUS       DAWGJV010000138         4871 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWGJV010000138 DAWGJV010000000
VERSION     DAWGJV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.138"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            66..326
                     /locus_tag="WHQ94_001733"
     CDS             66..326
                     /locus_tag="WHQ94_001733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128975.1"
                     /translation="MENAAASPAGHFRRHSRRRKCPTLKAQTHTQNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="WHQ94_001734"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="WHQ94_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ2128976.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1093..1482
                     /locus_tag="WHQ94_001735"
     CDS             1093..1482
                     /locus_tag="WHQ94_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ2128977.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEVRQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="WHQ94_001736"
     CDS             1560..2390
                     /locus_tag="WHQ94_001736"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ2128978.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="WHQ94_001737"
     CDS             2405..3862
                     /locus_tag="WHQ94_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ2128979.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILSAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..4292)
                     /locus_tag="WHQ94_001738"
     CDS             complement(3903..4292)
                     /locus_tag="WHQ94_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128980.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4474..4797)
                     /locus_tag="WHQ94_001739"
     CDS             complement(4474..4797)
                     /locus_tag="WHQ94_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2128981.1"
                     /translation="MNILASDRNYELNIEIFIDNIIHFQITDESYKVKFSEYLFENKE
                     KNDWDRNPAINYFFEIIDDSYMDWLKEESFDFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT         1203 a         1497 c         1031 g         1140 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcattt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacacaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc gccaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat aaagcagaat tctttaacga attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc gcactgattg ccctcgctct cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttacg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccaccggc ctcggcgtat tggcgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccggc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgtcggcg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagt gcgccaagtg
     1261 ttccaaaacc tgcaagccgt cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat tttgccgtct tcaacgaagt catggcggaa
     1381 tttatcgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctcgct gcccaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agcgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggtgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agggcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcata accaaagccg aatcgctcgc cggagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttggaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtac atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggtgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaactct ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatga gctggttcga tgccgtctgt cacgcaatgg cgaccctctc
     3061 actgggcgga ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 ggcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc tttccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gattggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaggccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cctgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcaggct ggaaatcttc accgtcttca tcctcttcac
     3841 ccccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatcttgtc
     3901 ctttaaagaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatatttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctttact aattttccac ataattattt acctttatcc attgcttccc
     4321 aaccgataca tccggtattt tagcttctcc ttttccgtca aacactcctt tggttacctg
     4381 ctttgtgtca aaaataccgt ctgaaagccg aatatcgttt cagacggtat tttgattgtt
     4441 gggtttttaa tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt
     4501 cgagctgata acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt
     4561 cttttcaaaa aaatcaaaac tttcttcttt caaccagtcc atataactat catctattat
     4621 ctcgaaaaaa taatttatag caggatttct atcccaatca tttttttctt tattttcaaa
     4681 taaatattct gaaaatttta ctttataaga ttcatccgtt atttgaaaat gaattatatt
     4741 gtcaataaat atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc
     4801 ttgttcggta acacaaattt tgtccacata aaatctgtga ttattgttag aaaattgtaa
     4861 tggtttaatc t
//
LOCUS       DAWGJV010000139         4704 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWGJV010000139 DAWGJV010000000
VERSION     DAWGJV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.139"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            45..449
                     /locus_tag="WHQ94_001740"
     CDS             45..449
                     /locus_tag="WHQ94_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ2128982.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            489..1673
                     /locus_tag="WHQ94_001741"
     CDS             489..1673
                     /locus_tag="WHQ94_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ2128983.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYFSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(1736..2269)
                     /locus_tag="WHQ94_001742"
     CDS             complement(1736..2269)
                     /locus_tag="WHQ94_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ2128984.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(2448..4703)
                     /locus_tag="WHQ94_001743"
     CDS             complement(2448..4703)
                     /locus_tag="WHQ94_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ2128985.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1169 a         1318 c         1184 g         1033 t
ORIGIN      
        1 aaattgccct accgtttttt aaacactttc aggaataagg aaaaatgacc gcccaaccct
       61 gccccatctg cacggcacaa gatgaagata ttttgctgca aacacccaag ctgcgcgtca
      121 tcgccgtcca taacgatagt ggttcgcctg cattctgccg cgtcatttgg cgtaagcata
      181 ttgccgaaat gaccgacctt tcggcagcgg aacgcggcga attgatggaa atggtgtaca
      241 aagtcgaagc cgctatgcgc caagtgtttc aacctgccaa gattaacctc gccagcttgg
      301 gcaatgtcgt gccgcacctg cattggcata ttatcgcccg ctttgaaaac gatgcgtcct
      361 tccctgcgcc gatttgggca aaccccgtcc gaaaacacgg tatgaccctg ccgcaagatt
      421 ggacggaaca gcttaaaaag ctgctttaag cccgccgatg ccgtctgaaa ccgtatgaaa
      481 gggaaattat gaccgaaccg acctcccgcc gtcgttttct gaaaacctgc accgccgccg
      541 ccggcgcggg actgcttcag gcttgcggca catccgccac atccgttccg ccccttccct
      601 cttcccattc tgttgtgaaa gcccgaaccg tgcctctcca aacgccacgc cgtcaaagtt
      661 cggacggcaa ccttctgcgc gttgtcgctt cgtcaggctt tgccgaagac accaaccgcg
      721 tcaacacagc cttaacccgc ctttacaatg ccggttttac cgtaaccaac caacaggcgg
      781 gcagccgccg cttccaacgg tttgctggta cggacgcaca acgtgtcgcc gatttccaag
      841 aggtcgcttc cggccgcgtc gccacgccta aagtgctgat gggcttgcgc ggcggttacg
      901 gtgcggcgcg gattctgccg catatcgatt ttgcttcgct cggcgcaagg atgcgcgaac
      961 acggcacgct gtttttcgga ttcagcgacg tatgcgccgt ccagctggca ttgttggcaa
     1021 aaggcaatat gatgagtttt gccggcccga tggcttatag cgattttggc aaacccgccc
     1081 ccggtgcgtt tacgatggat gcctttatca agggtgcaac ccaaaaccgc ctgaccgttg
     1141 atgttcctta tatccaacgc gccgatgtcg aaaccgaagg catattgtgg ggcggcaact
     1201 taagcgtcct cgcctcgctc gccggcacgc cttatatgcc cgacatcgac ggcggcattt
     1261 tgttcctcga agatgtcggc gaacagccct accgcatcga acgtatgctc aatacgctgt
     1321 atttttcggg tattttgaag aaacagcgcg ccatcgtgtt cggcaatttc cgtatggaaa
     1381 aaattcgaga tgtctatgat ccgtcttatg atttttccgc cgttgccaac catgtttcgc
     1441 gcacggcgaa aatccccgtg ctgacgggct ttcccttcgg gcacattgcc gacaaaatca
     1501 ctttccctct gggcgcgcac gcccgaatcc gtatgaacgg aaacagcggt tattcggtcg
     1561 cgtttgaagg ctaccccaca ctcgatgcgt ccgccctgac tttggatacc ctgctcccac
     1621 cgccggattt gcccatcttc cccgaaagcg gtgttgccga tatttcggaa taaacccgca
     1681 aacggacaaa tgccgtctga agccttcaga cggcatttcc caagacggcg gcagattaca
     1741 gcaatgcccg aatatcggct tcgatttctt cgggcgtaac actaggcgca aaacgctcga
     1801 ccacttcgcc gtcgcggttg acgaggaatt tggtaaagtt ccatttgatg tcgccttcgt
     1861 cgcgcttctc tcccaaagct gcgagcttca acacgaaatc tttaaacaga tgattgcctt
     1921 tatcttgcgg tttgacggat ttcaggtagg catacaaggg cgcggtattt gctccattga
     1981 cttcgatttt gtcgaaaatc ttaaacttcg tgccaaactt catcatacac acttgggcaa
     2041 tttctccgct gctttcggga gcctgttcgc ggaactggtt gcacggaaaa tccaaaatct
     2101 ccaagccttc tgcggtatat tgtgcataca gcttctgcaa agcctcgtat tgcggggtca
     2161 gaccgcaacg cgttgccgtg ttgacaatca gcagaacctt gccgcgatag cctgacaaat
     2221 caaccgcatt gccttccgca tctttcattt gaaaatcgta aatacccatt tttatcctta
     2281 tctgatgtaa accgatgcca tctgaaacgt gcttcagacg gcatgaaagc agcaattgta
     2341 tagccgatta aaataaaaaa tccacatcct tttccattcc cgtcccaatc cgcaataaaa
     2401 aactgcaccc gaaaacgggt gcagttgctc atttcatacc gcaaaactta tttgtcgcgg
     2461 ccgaatacga ttttagtggc ttggatggca acgcagattg caccgccgat gaagatcaag
     2521 tcagcagcag tacgaaccca acgcagagta tccaagattt ccatttgcag gaactcttcg
     2581 ctacgcgcat accacaggcc gtgcgtgata gaggcgtatg cctgaatcgc gccgacaggc
     2641 agcaggctga tggcaatcat accgaccaaa ccgccgttga gcagccagaa gccccaagtc
     2701 atcagtttgt cgtcaaactg cgcgttcggt ttcaaataac gggcaaccag caatacgaag
     2761 cccaatgcca agaaaccgta cacaccaaac aaggcggcgt gcgcgtgaac ggcagaagtg
     2821 ttcaaacctt ggatatagaa cagggaaatc ggcggattga tcaggaagcc gaatacgccg
     2881 gcaccgatca tattccaaaa ggcgactgcc acgaagcaca tcagcggcca acgcaggcgt
     2941 ttcgcccagt cggacaggtg ttggtaagac cagtgttcgt atgcttcacg gcccagcaac
     3001 accagcggca cgacttccaa agcggagaag caggcaccga ttgccataga ggcggaggta
     3061 gagccggaga agtacaggtg gtgcagcgta cccggaacgc cgcccaacat aaagatggcg
     3121 gcagcggcca aagtggaggc agtggcggta ctgcggcgga caaagcccat attgtagaag
     3181 acaaaggcaa aggcggcagt ggcaaatact tcgaagaagc cttctaccca caggtgaacc
     3241 acccaccaac gccagtattc cataacggca atcggggatt tttcaccgta gaacagaccc
     3301 ggcgcgtaga acacgcccac gccgaccata gaagctacga agatagccaa caggtttttg
     3361 tccacgcctt tttctttaaa ggcggaaacc gtgcaacgca acatcaggaa cagccataac
     3421 agcagaccga ccatcaaaag gagttgccag aaacgtccca aatcgaggta ttcgtaacct
     3481 tggtgtccga accagaagtt aaattccggc ggaaggatgt gcgtcaacgc gaagaagttg
     3541 cccgcgtaag aaccgccgac cacgatgaag agggcgatat agaggaagtt tacgcctgca
     3601 cgttggaact tgggatcttt gccgccgttg acaatcggcg cgaggaacaa acccgccgtc
     3661 aaaaagccgg ttgcaatcca gaagatggcg gattggatgt gccaagtacg ggtcagggcg
     3721 taggggaacc agtcggacat ttcaaagccc aacgcctcgt caatgccgta gaaaccctga
     3781 ccttcaacgg tgtagtgcgc agtcagaccg cccagcaata cttgtaccac aaacagggca
     3841 accgtcagga agacgtattt gcccaatgct ttttgcgaag gggtcagttg gattttggaa
     3901 atcgggtctt cagacggcac ttccacttcc tcgtgtttgg tcaggaagga ataaccccac
     3961 atcaacaaac cgatgcccat caagagcagt acaacactgg taaacgacca catgtagttt
     4021 tcagtagtcg gtacattgtt aatcaaaggc tcgtgcggcc agttgttggt gtaggtaaag
     4081 acctcgccgg gacggttggt cgaagcagac caagaagtcc agaagaagaa gtcgaacagt
     4141 ttttcacgcg cttcctgact tggcaatgtg ttgtttttca ttgcaaagtg ttcgcgggtg
     4201 gtttgcagcg cgggatcgtc gccgtaaaca ccgtggtaat aaggcaggat gctttcgatg
     4261 gctttcgcgc gcgtatcgct gatgacgacg ctgccgtctt ctttaatacg gctttggttg
     4321 cggtattcgt cagccagtcg ggttttcaga acggcttgtt cttcagggga aacttcatcg
     4381 aattttttgc cgtaagtctg ttgcgcggtc aaatccaacc aggcaaccaa ctcacgatgc
     4441 agccagtccg ccgtccagtc cggagcctga tatgcgccgt ggcccaaaat cgaaccgact
     4501 tccataccgc cggtagtctg ccatgcagac tgacctgcca aaatatcgtc tttcgtcatc
     4561 aagactttgc cggatgcgga aacgacctgt tcggggtaag gcggggcttt tttgtaaacc
     4621 tcgctgccca tatagccaag aatggtaaag cataccgcca gaacggcaaa cagcaagtac
     4681 cacagcttct tgtactgtcc catt
//
LOCUS       DAWGJV010000140         4697 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWGJV010000140 DAWGJV010000000
VERSION     DAWGJV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.140"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(110..1117)
                     /locus_tag="WHQ94_001744"
     CDS             complement(110..1117)
                     /locus_tag="WHQ94_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2128986.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTLSHKIDQRAFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDEEGNWSDVGLSSASFLGGLMGFQWGAALGVGAFGLLTGGAGWLVVGT
                     IIGGGSGAWWGSLGAEKAWRWFEDFLNLNRSDKFHVTDPLALDLDGDSIETVATKGLA
                     GALFDHRSQGIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTC
                     H"
     gene            2391..4523
                     /locus_tag="WHQ94_001745"
     CDS             2391..4523
                     /locus_tag="WHQ94_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ2128987.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT         1219 a         1025 c         1053 g         1400 t
ORIGIN      
        1 ctatacgaat aaaaaaatat cctacctagc ccgaaccttc ttataatttg tactatgtga
       61 tcattcattt caatatccat ttaagctttc aattaatttt ctatattttt caatggcatg
      121 tatcatttgg ttgatgatca agccatgtat gcaaagattg gaatatggta tctgtcgtgc
      181 taataatgcc cccgttactg tttaatttgc gcacaggaaa accgtcatat gcagcaatcc
      241 agcccgtggc ggtgcggata ccttggctgc ggtggtcgaa caatgcacct gcaaggcctt
      301 tggtggcaac ggtttctata ctgtcgccat ctagatctag ggcaaggggg tcggttacgt
      361 ggaatttgtc gctgcggtta agatttaaga aatcttcaaa ccatctccaa gctttttcag
      421 ctcctaatga tccccaccaa gctcctgagc cacctccaat tatagttcca actactagcc
      481 aacctgcgcc accagtaagt aaaccaaaag ccccaacacc caaagccgca ccccattgga
      541 agcccattag cccacccaaa aaagaagctg atgagagacc aacatcactc caatttcctt
      601 cttcatccaa ggctttccac caagcacttc ccatactcaa agttcccatg gcaacagttg
      661 ccccataagt aaacttaccc aaattcgctg ccataacttt tgattttgaa gcattctcta
      721 acgctgttgc cgcacgtctt gcatattgct gcttgttata gttgctagca ttcaatgctt
      781 tttgcaaatc actttgtact tttcttgcaa caaatgcctc ctttgaatta ccacgaaatt
      841 tactagcagc tcttccaatt ccttctttta agcttttttg tgcctcttct gctgtgcgtt
      901 ggaattctct tgccttagca gacagacgct caccctccct taaaaatgct cgttgatcta
      961 tcttgtgcga aagggtcaaa tattcttcgt acattgagtt aatcgtacca atcgccctaa
     1021 ccacattttc atcagaaaat atggaaataa tagcatccct atacgcacct agagtaatat
     1081 tgtttctatt attagttata gcattattcg aatacataat agcacctcca aacttagaac
     1141 tgaatacgtt tatattctct gtgtaataat atcaaataat taattaatgg ataagtaagt
     1201 ccgaaaaaat aacaactaaa ataagcaata tactgattct ctcctttatt aattagatat
     1261 attgaaaaac aaataaaaaa taaacagaaa acattcatgt gaaaaagcca cctcatattc
     1321 ttatttataa gtttaaaata actaggcatt gtatatttaa aaatcggtac atccaacgga
     1381 aacaaaccac ataaaaattt ttctttattc tttattaaag acatagtttt atttttcaaa
     1441 attagtccag ataatatact aacaattact attaaaacaa taagcacagc gtaatttttt
     1501 ataaaattca ttaaaataat aacgaaaata ctaaatagat tacatactgt aaattgaaat
     1561 ataaaatatt cagtatttaa tccattttca accgcgagtt ttttaaaata tccttgctcg
     1621 aaaaaattaa aaaaatccat taatactttc ctctcaagaa aactaatttt agtttagtta
     1681 agataaaatg caatattacc ttttctccat aaaagtatgg agcttatata cataaattat
     1741 gtgcgaggta gcctgaaaaa tatccaatac aagatagtag gagatgtttc ccttatcact
     1801 attggccaat acccaattgg caggcactct atttccaaaa ttttctcaag aatagttaaa
     1861 tattattaat tagtttaaaa acaataacat acgtttgtat ttatccgttt tggtctgcct
     1921 gaaacagact ctatgtccat acacggacat gtagacagtt tccttgttat tttggttgct
     1981 cgtcattagt gatgatactt tactcctctc ttaaaaaatc gtcaagaact gttatcaatc
     2041 tttaaagtct tttttaaaca aatatatata cgttcaggct gtctgaaaca caaaaaaatg
     2101 atgttacccg accgtgacag agtcggggca gccggtacct taaccctgca ccttaaccct
     2161 gtgccttaac ggtatgcagg aggcacgaga ggtgcgaggc atcctcatct aactcgattc
     2221 ctaagccgtt ttaccttcct gaaacgagaa cttttccata ctttctcttt acaattccca
     2281 tatacatcct tacaatgtgt tctgctgttt tatttatata tttctttcta ctgaatatat
     2341 tccttttcca gccagccgtt atccacatcc aatctaacta tattttattt atgtctatcg
     2401 tatccgcacc gctccccgcc ctttccgccc tcatcatcct cgcccattac cacggcattg
     2461 ccgccaatcc tgccgatata cagcatgaat tttgtacttc cgcacagagc gatttaaatg
     2521 aaacgcaatg gcttttagct gccaaatctt tgggattgaa ggcaaaggta gtccgccagc
     2581 ctgtcaaacg tttggctatg gcgactttac ccgcattggt atggtgtgat gacggcaacc
     2641 attttatttt ggctaaaaca gacggtgggg gtgagcatgc ccaatatcta atacaggatt
     2701 taactacgaa taagtctgcg gtattgtctt ttgccgaatt ttctaacaga tattcgggca
     2761 aactgatatt ggttgcttcc cgcgcttcgg tattgggcag tttggcaaag tttgacttta
     2821 cctggtttat tccggcggta atcaaatacc gccggttgtt ttttgaagta ttggtggtgt
     2881 cggtggtgtt gcagctgttt gcgctgatta cgcctctgtt tttccaagtg gtgatggaca
     2941 aggtgctggt acatcgggga ttctctactt tggatgtggt gtcggtggct ttgttggtgg
     3001 tgtcgctgtt tgagattgtg ttgggcggtt tgcggacgta tctgtttgca catacgactt
     3061 cacgtattga tgtggaattg ggcgcgcgtt tgttccggca tctgctttcc ctgcctttat
     3121 cctatttcga gcacagacga gtgggtgata cggtggctcg ggtgcgggaa ttggagcaga
     3181 ttcgcaattt cctgaccggt caggcgctga cttcggtgtt ggatttggcg ttttcgttta
     3241 tctttctggc ggtgatgtgg tattacagct ccactctgac ttgggtggta ttggcttcgt
     3301 tgcctgccta tgcgttttgg tcggcattta tcagtccgat actgcggacg cgtctgaacg
     3361 ataagttcgc gcgcaatgca gacaaccagt cgtttttagt agaaagcatc actgcggtgg
     3421 gtacggtaaa ggcgatggcg gtggagccgc agatgacgca gcgttgggac aatcagttgg
     3481 cggcttatgt ggcttcggga tttcgggtaa cgaagttggc ggtggtcggc cagcaggggg
     3541 tgcagctgat tcagaagctg gtgacggtgg cgacgttgtg gattggcgca cggctggtaa
     3601 ttgagggcaa gctgacggtg gggcagctga ttgcgtttaa tatgctctcg ggacaggtgg
     3661 cggcgcctgt tatccgtttg gcgcagttgt ggcaggattt ccagcaggtg gggatttcgg
     3721 tggcgcgttt gggggatatt ctgaatgcgc cgaccgagaa tgcgtcttcg catttggctt
     3781 tgcccgatat ccggggggag attacgttcg aacatgtcga tttccgctat aaggcggacg
     3841 gcaggctgat tttgcaggat ttgaacctgc ggattcgggc gggggaagtg ctggggattg
     3901 tgggacgttc ggggtcgggc aaatccacac tcaccaaatt ggtgcagcgt ctgtatgtac
     3961 cggagcaggg acgggtgttg gtggacggca acgatttggc tttggccgct cctgcctggc
     4021 tgcggcggca ggtcggcgtg gtcttgcagg agaatgtgct gctcaaccgc agcatacgcg
     4081 acaatatcgc gctgacggat acgggtatgc cgctggaacg cattatcgaa gcagccaaac
     4141 tggcgggcgc acacgagttt attatggagc tgccggaagg ctacggcacc gtggtgggcg
     4201 aacaaggggc cggcttgtcg ggcggacagc ggcagcgtat tgcgattgcc cgcgcgttaa
     4261 tcaccaatcc gcgcattctg atttttgatg aagccaccag cgcgctggat tatgaaagtg
     4321 aacgagcgat tatgcagaac atgcaggcca tttgcgccaa ccggacggtg ctgattatcg
     4381 cccaccgtct gtccaccgtt aaaacggcac accggatcat tgccatggat aaaggcagga
     4441 ttgtggaagc gggaacacag caggaattgc tggcgaagcc gaacggatat taccgctatc
     4501 tgtatgattt acagaacggg tagaaggcgg gggatgggcg tgtaggctgc ttttttcgta
     4561 acgttctgcg ttttcagacc gtcgtttact gggtttactg actgaaatga aaccaaagtg
     4621 caggctgccg gaaagacttt ttctgtcttt ttaggcagcc tgcacttctt tttcactcat
     4681 ctagagacct ttgcaaa
//
LOCUS       DAWGJV010000141         4647 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWGJV010000141 DAWGJV010000000
VERSION     DAWGJV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.141"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            107..1048
                     /gene="miaA"
                     /locus_tag="WHQ94_001746"
     CDS             107..1048
                     /gene="miaA"
                     /locus_tag="WHQ94_001746"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ2128988.1"
                     /translation="MPTPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1052..1771
                     /locus_tag="WHQ94_001747"
     CDS             1052..1771
                     /locus_tag="WHQ94_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ2128989.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1776..2264
                     /locus_tag="WHQ94_001748"
     CDS             1776..2264
                     /locus_tag="WHQ94_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ2128990.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2330..3082
                     /gene="rlmB"
                     /locus_tag="WHQ94_001749"
     CDS             2330..3082
                     /gene="rlmB"
                     /locus_tag="WHQ94_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ2128991.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3144..4535)
                     /locus_tag="WHQ94_001750"
     CDS             complement(3144..4535)
                     /locus_tag="WHQ94_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ2128992.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         1160 a         1491 c         1126 g          870 t
ORIGIN      
        1 gcatacggta aacacgcccc cttgccgtcg gcgtgcttcc gcaaaccaaa atataacgtc
       61 ggcacgcttt ccgctataat ccgtattttt ccaaacctga aacgttatgc cgaccccgaa
      121 agcctttgcc ctgctcgggc cgaccgcctg cggcaaaacc gcgctcgccc tcaaaattgc
      181 cgaaaccttg cctgtcgaaa tcatcagcct cgattccgcg ctggtttacc gcgatatgga
      241 catcggcacg gcgaaaccgt ctcatgccga gcgttccttt gtaccgcacc accttatcga
      301 catcattacg cctgttcaga catacagtgc cgcccgtttt gtcgaagact gcacgcgtct
      361 gattggcgaa atcacggcgc gtgggaaata tccgctgatt gtcggcggca cgatgatgta
      421 tttccgcgcc ctgacccaag gtttgaacga tttgcccgaa gccgacgcct gcctgcgtgc
      481 cgacttggac gaacaaaaac aaatgtacgg cttggatttc ctctaccgca ccctgcaaaa
      541 agtcgaccct gaaacagcat gccgtctgaa accgaacgac agccagcgta tcggacgcgc
      601 tttggaagtt tattatttaa ccggcaaacc gatgagtacg catctcggca gcccaacctc
      661 ccatacgctc cccttcgatt tgcataccgc cgccctgatt cccgaaaacc gcgcccgcct
      721 gcatgaaaac atcgccctgc gtttccacct gatgcttgaa cagggcttta tcggcgaagt
      781 agaaaacctg cgccgcctct atcccagcct gaccgccgac tcccctgcca tacgctgcgt
      841 cggctaccgt caggcatggg aatatctgga cggaaaaacc gatttcccgg catttgtcga
      901 gaaaggcatt gccgccacgc gccaacttgc caaacgccaa ctgacttggt tgcgcaaaac
      961 acctttagac tgcgttaccg acccgttttc agacggcaat tcatgcacac gcctgattga
     1021 ggcggcaaaa cggtttttcg gcgcataaac tatgctgacc acgccgccgc tcgcccccaa
     1081 aaccgtcgcc gccctacaca ggctcggtat ccgtacgctc gaagaactgc ggcaaaacgg
     1141 cgcggttcaa accttcttgt tattgaaggc atcaggtttg accctcacga aaagcacgct
     1201 gtggcagctt cagtcgctgc tggacggcac gccgccgcaa gaaatggcgc aacacgataa
     1261 aacccgcctg cttgccgagt tgaaaaacca tccgcccgtc gccgcctttc ccccgcaagg
     1321 cgaaatggcg catttcatgg gtttggcatt ggaacaggcg aaactgtccg cattaatggg
     1381 cgaaataccc gtcggggcag tcatcgtttc agacggcaaa atcatcgcat cggcacacaa
     1441 cacctgcatt gccgactgca atgtcagccg gcacgccgaa atcaacgcct tggcacaggc
     1501 aggcagcgaa atgcaaaact accgccttga cggatgcgac atctacatca ccctcgaacc
     1561 ctgcgccatg tgcgcgtccg cactgataca ggcacgaatc aggcgcgtga tctacggtgc
     1621 ggcagaaccc aaaaccggcg cggcaggaag catcgtcaac ctgtttgccg acaaacgcct
     1681 caatacgcac accgccatac ggggcggaat cctgcaagaa gaatgccgcg ccgtattaag
     1741 ccgttttttc caaaacaaaa gaaaaggttg aacctatgac cgccttgctc gtcatcctcg
     1801 ccctcgccct gatagccgcc ggtacggcgg gcatcgttta ccccgccctg cccggattgg
     1861 cattgatgtt tgccggaacc tggctgctcg cctactccgg cggctaccaa atctacggcg
     1921 cgggcgtttt gtggacggtc ggactcatca gccttgccgg catactggcg gactatgtgg
     1981 caggcatatg ggggacaaaa tataccggag cgggcaagct cgccgttcgc ggcgcattgg
     2041 tcggcagtat catcggtatg tttttctccc ttcccggact aatactcggt ccctttatcg
     2101 gcgcggcggc aggcgaactg atcgatcggc gcaatatgct tcaggcaggt aaagcgggct
     2161 tgggtacgct gttggggctt gtcgtcggca cggcgttcaa aatcggctgc gccgtatcca
     2221 tcttgtttat cctgttggtg aaatacatcg cctacctgtt ttaacccctt cagacggcat
     2281 accggcaaat tgctacaatg ccgtctgaac ccttttcaca aagcaccata tggcaaacca
     2341 aagacctatc tacggcttcc atgccgtcaa cgcccgattg tggcaaaacc ccaaatccat
     2401 cgtcgaactc tatgtccaag aaggcaaaaa cgatgtacgc acgcgcgaag tgttggaaaa
     2461 ggcggcaaac gaaaacatcc gcgtatattt tgccgatgcc gaccgcctca acgccatcag
     2521 caaaggtgcg cgccatcagg gcgtggtcgg atttatcgat gcctccaaaa accacgtcca
     2581 cctcgaagac gtattggaaa acttaagcga accgccgctg ctgctgatac tcgacggcat
     2641 caccgatccg cacaacctcg gcgcgtgcct gcgtaccgcc gacgcaatgg gcgtacatgc
     2701 cgtcatcgca ccgaaagaca aaagcgcggg gctgaacgcc accgtcagca aagtcgccag
     2761 cggcgcggcg gaaaccgtcc cctacatcac cgtaaccaac ctcgcccgca ccctgcgcga
     2821 gctgaaagaa tacggcattt ggatcatcgg caccgatatg ggcggcaacg ccgaccttta
     2881 ccattgcgat ctgcccgaca gcgcggcatg ggtgatgggc aacgaaggcg acggtatgcg
     2941 ccgcctgaca cgcgaacatt gcgacatgct ggtgtccata cccatgttcg gcacggtcga
     3001 aagcatgaac gtctccgtgt ctgccggaat ggtattaagc gaaacccgcc ggcagcgcgt
     3061 attaaaaaac gaaaaagcgt aagcctcaaa aacaatgccg tctgaaacag ggattctgtt
     3121 tcagacggca ttcgttttca aaatcagtgt tccaagtggt cggtatcaaa cttgaccgcc
     3181 gcctcggttt tatcttcggg caagaccaaa ttcagcaaga ctgccgtaat gccgccggcg
     3241 gaaatagagt tttggaacaa gacaggcagg tttttaaaca cttccggctc aaacgcgaca
     3301 cccaagccca aaccgaccga cgttgccgca attaccgctt cgcgcctgcg gatgccgtgg
     3361 ctgaccaaaa tccgcacgcc cgcaatcgca atcaagccga acatcaaaac catcgcgccg
     3421 cccaacaccg gactcggaat cgtcgtaaac gcgcgtccga caacggggaa cagacccaac
     3481 agcaccaaaa tcacggcaat atatttgccc acatggcgcg aagccacgcc ggtcatctga
     3541 atcacgccgt tgttttgcgc aaacgtcgtc agcggcagcg aacccaaagc cgtcgcaatc
     3601 accgacacca agccgtcagc caacacgccg ccgcgcaggc gtttggtgta ttcctcgcct
     3661 tcaatcggct gatcggacac cattgccgtc gccgtcaaat cgccgaccgc ctcaaacacg
     3721 ctcaacagga aaatcgcgcc cgccacaata aatgcgtgcc agtcgaaagc aaaaccgtat
     3781 ttaaacggta cgggcagcgt aaccagcggc aggttttgca gcgcggaaaa atccaccttg
     3841 cccaaaaaca gcgcgacaat gtaaccggca atcagaccga ccgcgatgcc gctcatccgc
     3901 aacagcgggt ttttcatgca gttgaacacc agcacaatca gcaacaccag cgaagccagc
     3961 cccaagtttt ccatcgagcc gaacgtgccg tccgccttcg cgccgaagcc gccgccgaaa
     4021 tcggtaatac cgacgtgtac caaactcaag ccaatgagca tcacgaccac gccgctgacc
     4081 gtcggcgtaa tcactttttt caaatacggc aaaagccacg ccgagaaaca caccaaaaac
     4141 gcgccgacaa acgatacacc caagagcgtc gaaatcatcg catccttagt caaaccgccc
     4201 tccttcatcc ctgcgcccag cgcaatcatc acggtaacga acgaaaaatt caccgactgg
     4261 atggacagca tccccgaacc gaccggcccg aagcggttga cctgcaaata agtgccgaca
     4321 cccgacgcaa ccatcgccat cgacacgaga tacgccgtca tctccaccgg caattccagc
     4381 gcgccgccca caatcagcgc gggcgtaatc atcggcacaa aaatcgccaa aagatgggta
     4441 accgcgctca agagcgcatt accgaacggc ggcctgtctt ccaaaccgta caccaaatca
     4501 ggcgcatccg cacctttgcc caactgaccg ctcattgtat ttccttattt taaaggttga
     4561 aaaatccaag cgcggattgt agcgaatctg ccgcacggct tcaatttgca tttcagacgg
     4621 aatccgtctg gtccacacgt ttaccac
//
LOCUS       DAWGJV010000142         4602 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWGJV010000142 DAWGJV010000000
VERSION     DAWGJV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.142"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(207..1145)
                     /locus_tag="WHQ94_001751"
     CDS             complement(207..1145)
                     /locus_tag="WHQ94_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2128993.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAECLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1154..2029)
                     /locus_tag="WHQ94_001752"
     CDS             complement(1154..2029)
                     /locus_tag="WHQ94_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ2128994.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2218..2294)
                     /locus_tag="WHQ94_001753"
     tRNA            complement(2218..2294)
                     /locus_tag="WHQ94_001753"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2258..2260),aa:Met,seq:cat)
     regulatory      2444..2543
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2634..4535
                     /gene="thiC"
                     /locus_tag="WHQ94_001754"
     CDS             2634..4535
                     /gene="thiC"
                     /locus_tag="WHQ94_001754"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ2128995.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1111 a         1303 c         1218 g          970 t
ORIGIN      
        1 tttgcgggaa tgacgatgaa aagatttttg ttgtttcgga taaatttttg ccgtgttggg
       61 ttctagattc ccgcttttgc gggaatgacg gcagggtggt ttctgttgtt tcggataaat
      121 tttgcagccc tgataaaaaa tatggctgct ttggtaaaaa atgccgtctg aaaggttttc
      181 agacggcatt ttgtttttaa gaagcatcag cggaagcgga cgatttccct gtcttcgata
      241 tggatgcgta ccgtatcgtt tcccgatatt tcaccggcgt gcggcatatc gaggttcagc
      301 cacaggatgc cgtgttccgg atggaggacg gacaggctga acgattcggg caaacaggta
      361 cgggataata cgcggcactc catgccgtct tggtcgaaac gcaccgcatg ttgcggaata
      421 tggcggtcat cgtcggtatt gggcaaaccc atcagtcggg cgacctgcac gccggcaggc
      481 gtttgaacca aggtttcggg cgtaccgcat tgaaggattt tcccctcgtg catgacggcg
      541 atttcgtccg ccgccgtgca ggcctcttcg ggcgaatgcg ttaccaaaac ggcagggatg
      601 ccgcccttgc ggatgcgttc ggcagtcatg cggcgcagcg tgccgcgcaa atgcgtgtcc
      661 aaactggaaa acgattcgtc caacagcagc agggaagggc ggacaaccaa agcgcgcgcc
      721 agtgccaacc gttgtttctc gcctccggaa agtttttcag gcttgcggtg cacctcgttt
      781 tccagtccga cttcggcaag tgccgccatg gcaaggcatt cggcctcggc tttcggcatt
      841 ttttgcattt tcaaaccgaa tgccgcattt tccagcgcac tcatatgggg aaacagcgcg
      901 taatcttgaa acatcagcga gatacggcgt ttttcgggcg gcatacgggt aatgttttcc
      961 ccgttcagct gaatttcgcc gccgtccggc cggacgatgc ccgcaatcat attcagcagg
     1021 gtggattttc cgcagcccga ccgccccagt acggcgagta ttttgccgcg cccgacagtc
     1081 aggcagatgt tgtcggcaac cgttttgccg ccgaagcgtt tgcagagtcc gttcagttca
     1141 agcatggcgc atcctataaa cgtatgccgt ccagccactc ggacagcgga tgggcggcat
     1201 caatcagacc gtaacggcaa aacgcgtcca gcgtaaccag ttgcgcgtcg tgcatcatgt
     1261 ttcccgacaa catggtggca accaagctgc cgatgtccat tttttcaaaa cacgcaacct
     1321 cgccgtcctg attttcaggc aggaaggttt cgggtaggac ggcatcgaat acatacagga
     1381 tttcattgtg cacaccccgg ctgacggggc gcaggctgtg cagccgggat accgggcgga
     1441 tgagcggaaa cagcgtttga tccaaacccg cttcttcgct gctttcgcga cacacggctt
     1501 cagacggcaa ttcaccgctg gaaacaccgc cggcggcagt attgtcgagt ttgtcgggat
     1561 cgactgcttt gtgcggactg cgcctgccta tccaaaaatg ccatctgcca tccgattcag
     1621 tcagaccgtt gagatggacg gcgcggctga gcagtccgaa cggacggaaa gcggcgcgtt
     1681 cgagtgagaa caagatattg ccgccgccgt cggtcaggtc gaaacactcg ttgcgccatc
     1741 cgtgaagcag ccccgccttg ttccatgtgc gggcgaggtg ctgcaagcgg ccgcccatat
     1801 ctgaccagcc gtccgcattc aggaaaatgc cgtctgaaga ctccgagcag cctgcctccc
     1861 agtctttttt gacgcgttcc gcccattccg gcgacagatt gcccaaaggc agacggttca
     1921 gatacagcgt tttccagcaa ctttttgcac cgtaacttgc ttttgcccac tcgaacaggg
     1981 catcaaggtc ttgtttgctg acggattcgg taaaacggac ggtcggcata gtcggctttc
     2041 ttgaacataa tgcggaagat tgtagccaaa ccgcctgaat ttgcaaccgc aaacagacgg
     2101 cgggctgccg ttccgatatt gcggcaggaa ggaaaatccg gcaaaaaacg gaggcggcag
     2161 gaaacaggaa acaggcaaaa caaaacccct cggcattgaa ccgaggggtt taatatttgg
     2221 ttgcgggagc aggattcgaa cctacgacct tcgggttatg agcccgacga gctaccatgc
     2281 tgctccatcc cgcgtcagaa gatggaacta tacggcagat tttttatgtt gtcaaacatt
     2341 atttccgcga aaaatcataa aattttgccg tccggcggtt tatgcctgat ttgaaatatt
     2401 attttcttta caaaagttca tgttcgtgat ttaattttgg ttaacattga aacaggggtg
     2461 ctgcctgatg ttcaggcggc tgagaaatac cctttacacc cgatcgggat aatacctgcg
     2521 tggggagttt tcacggattc tgtctttcag acagcattgg ttttcaaatg ccgtctgaaa
     2581 acgcaaaacg ctcctgtttc tttattctaa acgagaaaac aggagcattt tttatgacta
     2641 cgccaaaaaa aaccgccaaa acttccggca acgaagcgcg cgaacttgcc gacttgagcg
     2701 aagacatcgg tatccgcttc aaatatccga actcggaacg cgtgtatctg caaggcagcc
     2761 gcgacgacat ccgcgtgcct ttgcgtgaaa tccgtcagga cgacacctat acggcgcaag
     2821 gcgcggaagc caacccgccg attcccgtct atgacaccag cggcgtgtac ggcgatccag
     2881 cggcacacat cgacctgaaa caaggtctgc cgcacatccg caccgcatgg ctgaacgaac
     2941 gcggcgatac cgaaatcctg cccaagctct ccagcgaata cggcatcgaa cgcgcacacg
     3001 atccgaaaac cgcccatctg cgcttcaacc aaatcacccg cccgcgccgc gcgaaaagcg
     3061 gtagcaacgt aacccagctt cactacgcgc gccaaggcat tatcacgccc gaaatggagt
     3121 ttgtcgccat acgcgaacgt ttaaaattag acgaattgtc ccaaaaatcg gaatacgcca
     3181 aactcttgaa acaacacgca gggcaaagtt tcggcgcgaa catcccaacc cgtcccgacc
     3241 aaatcacgcc cgaattcgtg cgccaagaaa tcgccgccgg acgcgcgatt atccccgcca
     3301 acatcaacca ccccgaactc gaaccgatga ttatcggccg caacttccgc gtcaaaatca
     3361 acggcaattt gggcaactcc gccgtaacct ccagcctgac cgaagaagtc gaaaaaatgg
     3421 tgtggtcgct gcgttggggc gcggacacga ttatggattt gtccaccggc gcgcacatcc
     3481 atgaaacgcg cgaatggatt atccgcaacg cgcccgtccc catcggcacc gtgccgattt
     3541 accaagcgtt ggaaaaaacc ggcggcatcg ccgaagattt gacttgggat ttgttccgcg
     3601 acaccctcat cgaacaggcg gagcaaggcg tggactattt taccatacac gcgggcgtgt
     3661 tgctgcgtta tgtgccgatg accgccaacc gcctcaccgg catcgtctcg cgcggcggtt
     3721 cgattatggc gaaatggtgt ttggcacatc atcgggaaaa tttcctctac acgcatttcg
     3781 acgaaatctg cgaaattatg aaagcgtatg acgtatcgtt cagcctcggc gacggcctgc
     3841 gccccggctg cattgccgat gccaacgacg aatcccaatt cgccgaactg cacaccttgg
     3901 gcgaattgac cgataaagcg tggaaacatg acgtacaagt catgatcgaa ggccccgggc
     3961 atgtaccgct gcaacgcgtc aaagaaaata tgaccgaaga gctgcaacac tgttttgaag
     4021 cgccgtttta cacgctcggc ccgctcgtta ccgacatcgc acccggctac gaccacatca
     4081 cctcgggcat aggcgcggcc aatatcggct ggtacggcac ggcgatgctt tgttacgtta
     4141 cccccaaaga acatttgggg ctgcccgaca aagaagacgt gcgcaccggc atcatcacct
     4201 acaaactcgc cgcccacgcc gccgatctcg ccaaaggctg gccgggcgca caattacgtg
     4261 acaacgccct gagcaaggcg cgtttcgagt tccgttggcg cgaccaattt cgcttaagcc
     4321 tcgaccctga acgtgccgag agcttccacg acgaaaccct gcctgccgaa ggcgcgaaaa
     4381 tcgcccactt ctgctcaatg tgcggcccca aattctgctc gatgaaaatc acgcaggaag
     4441 tgcgcgacta cgccgacaag caaaaagccc aacggcaggg catggaggaa aaagcggtcg
     4501 agttcgtcaa aaaaggcgcg aagatttaca gttgaaacgt caagcaaaaa atgccgtctg
     4561 aaaaccggaa aaaaggcttc agacggtatt ctttcgcttg tg
//
LOCUS       DAWGJV010000143         4576 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWGJV010000143 DAWGJV010000000
VERSION     DAWGJV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.143"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(245..352)
                     /locus_tag="WHQ94_001755"
                     /pseudo
     CDS             complement(245..352)
                     /locus_tag="WHQ94_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="WHQ94_001756"
     CDS             complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="WHQ94_001756"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ2128996.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1150..1941)
                     /gene="recO"
                     /locus_tag="WHQ94_001757"
     CDS             complement(1150..1941)
                     /gene="recO"
                     /locus_tag="WHQ94_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ2128997.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="WHQ94_001758"
     CDS             complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="WHQ94_001758"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ2128998.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3192..4373)
                     /locus_tag="WHQ94_001759"
     CDS             complement(3192..4373)
                     /locus_tag="WHQ94_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ2128999.1"
                     /translation="MPSTHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     L"
BASE COUNT         1028 a         1315 c         1246 g          987 t
ORIGIN      
        1 ctatattaca taatcatcgc ctcaatattt aatatcccaa aatatagatt taaaaccatc
       61 gcctttgtgg tttgtcagca ttcctaattg agatagaaag tcaaataatt tcttgtttct
      121 ccggctgcca tcaagatggt ttagattaag acctcgaaca atacaatacc caatactctg
      181 tactgtattg tgaatttctt ctatcaaact attattgttt gcagataaaa tatacttttg
      241 attcatatag tgaattaaat ttaaaccagt acggcgttgg cttgccttgc cgtactattt
      301 gtactgtctg cggcttcgcc gccttgtcct gatttaaatt taatccacta taacatatcc
      361 ctgtcgtgaa atgctgtctg aaagggtttg cctgccctta cggcagcagc cttgccctga
      421 acatcgcctc cttcatttgg cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa
      481 tcagcgaatg cccgatgttc agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt
      541 tgtgtatggt cagtccgtgt ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg
      601 cgccgttttg cagacgctcg aattgcttga gttgttcggc gtggctgcgc gcgtcggcat
      661 acgcgcctgt gtgcagctcg acaacgggcg cgccgacatc ataggcggct tggatttgcc
      721 tgtcgtcggc atcgataaag agagagactc ggatgcctgc gtcggtcagg attttggtga
      781 acccggcgat tttttcctgt tgcgccaata cgtccaagcc gccttcggtc gtgatttcct
      841 gacgtttttc aggcacgatg cacacgtctt ccggcatcac tttaagcgcg ttttcgagca
      901 tttcttccgt caacgccatt tcaaggttca ggcgcgtgcg gatggcgttt ttgacggcaa
      961 acacatccgc gtctttgatg tggcggcggt cttcgcgcag gtgcatggta atcaaatccg
     1021 caccgtgcgt ttcggcaacc agtgccgcct ccacggggct gggataagtc gtgccgcgcg
     1081 cattgcggac ggtagcgatg tggtcgatgt tgacacctaa aagcataatc tttcctttta
     1141 tttctgcctt cagacggcat ttgaagccgt gccgtccgaa gtcgggacgg tttcccgggc
     1201 ggtttctttg cggtcaaact gccgtatctg ttccaacacc tgccgcgatt tcagcccctc
     1261 gggcaacagg tggcggataa aaagccgtgt gattttcaat gcctgttgca ggctttcggc
     1321 ggtgcggaaa ctgccttcgc gcaaatcgat caggctttgc ccttcgacaa ttgcgccggc
     1381 ttcgtgcgaa agcgcgtctg cgtcctcgca tacgggcatg accgcctctt cgggcatcag
     1441 ccggtaggtt ttgtccgcca aaatatcgcc gcccgtgccg tcggcgtgca aatcgggggc
     1501 aacgcccaac aggttcagca gccgccactc gaaacggcgc aagtcgtcga tataagcggc
     1561 tttgcagcac accgcctcca tcacttccgc caacgcgtcg tataactcgg gcaacggatc
     1621 ttcgcggacg gtcaacttca gcatcaactc gttcacatac aatccgctga acaaagccct
     1681 accctgcggc tgcggccacc cgcctatcca ttcggcgcga tgtagggtct tcaactcttg
     1741 cgaaccatac caagacgcac tcaccggcac aaacggcacc aacacgccgc gcaattcgct
     1801 ctgccgtttg cgcgcgcttc ttgccaaaag tgccacacgc ccgtaacggc ggctgaacgc
     1861 ttcgacccgc aggctgcttt cgcgccaggg cgaggaagcc aacaggaaga cgggttcgtg
     1921 gttgactcgg tgttcggaca tcatgcctga aatgccgtct gaacgctgcc gccgctacaa
     1981 aacggcggtc gggtacgaac cgatgacttt gacgaacgaa gcgcgttcgc ccaagcgttc
     2041 caatgccgtc tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga agaacaggta
     2101 ttcccacaaa acggatttac tcggacggct ctcaaacttg gtcatggaaa tacccgactc
     2161 agtcagcggt tgcagcagcg aggcaaccgc gcctgcccgg ttgggcgcgg aaacggccag
     2221 cgaagtcttg tcgctgccgc ttgcaccggt ttcgtgatgt cccatcacca agaagcgcgt
     2281 ggtgttgttc ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt agatttccgc
     2341 cgccgtgcgt ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa ccagccttgc
     2401 ggcttcggca ttgctggaca cggcaatccg ttcggcgttg ggcaggtgtc tgcccaacca
     2461 gtcgttgcac tgcgccaacg cctgcgcgtg ggaaaagact ttggcaatgc cttcggtgct
     2521 gccgttgttt ttacgcaaaa ggttgtggtg gatgcgcaaa acgacttcgc cgcacgcctg
     2581 caacgcggta acggcaagca aatccaacgt gcgcccgacc gagccttcgg tcgagttttc
     2641 cacgggcgcg accagataat ccgcctgacg ggtttcaacc tgcttgaagc agttgtctat
     2701 ggtcggacac gccatggtgt gcgcggcgtg tccgaaatgc ttgatcgccg cctgctgggt
     2761 aaacgtgccc tgcggcccca gataggcgat ggtcagcggg cgttctacgg cgaggcactc
     2821 gctcatcact tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc ctttgttcaa
     2881 atcctgaatg cggcgcaaca cggcgacttc gcgttcgggg cggtacactg cgcccgtgcc
     2941 tttcagctcg ccgatggcgt gggcgtgttg cgcacgttcg ttgagcaggc gcaggatttc
     3001 ggcatcgatg gtgtcgatgg cgttgcggtg gggaaggagg agttcgtcga tagtttggga
     3061 cataatttcg cctgaacagt aatttatcgt gcattctaac atcaaatgcc gtctgaaacg
     3121 gtttcagacg gcattttccg ttaaaggtat tcgctttgcc cgttttcttt ccacgcacga
     3181 tgcgagcaga gctacagaag cgcaatgccg caagaggtgg acgcggtcat cgttgccgcc
     3241 atcacggttg ccgaaccgtc gtgcaagaag gtcgccgcca tacccacccc cgcgccgatt
     3301 aaagattgga atacacccaa tacggcgttt gcgctgccgc cctcttcttt gaaataggac
     3361 ataaaacacg cctgcgtgtt tgcaccgacc aagccctgcg taccgacgga aaacatcacg
     3421 cacgcgacca gcagccaaaa cgggggcaac ccgaaaaaca gcacggcggc gagttgggac
     3481 aggttggcgg caaactggac gacaatcccc cacagcagga tgctttgcgg atgcgcgccg
     3541 gttttgagcc gccacgcagt aacgcggttg aaaaacatca tcgtgatgat gttgagcgca
     3601 aacgcccaag cgtattgatg cggcgtaacg cggtaaagtt gctggtacac gaaggacgat
     3661 tcggtcaaaa aagcaaacat cgagccgaaa ctgaatgcct gaaaaaacag ataacccatc
     3721 gcggcacggg ttttcaatac gcgcttgaac cgccccgcca ccagcccgaa cacgtcccgt
     3781 ccgattttgc cgccgacggc gggcttgggc aggaaatact gtaccaaacc gagcagcacc
     3841 agcgaatacg ccgccagaaa aacaaaaatc gcctgccagc cacccaagcc ctgcaacaat
     3901 gcgccgacca tgggtgcgac cagcggcaca accatcaaaa tgatgccgat aagggcaaac
     3961 atctgggcgg ctttgcgtcc ggaataataa tcgcgcacca ttgcgccgac gatgaccaca
     4021 gtcatgcccg caccgaatgc ctgcacgacg cgcaggttga ggagctgttc ggcactcgaa
     4081 gcaaatacga tggcggcaac ggcaaggcag tagacggcca gtccggtcag cgcgacgggt
     4141 ttgcgccctt tgatgtcgga caccgaaccg ccgaccacct gtccgaacgc cgtgccgaac
     4201 ataaacaaac tcaaactctg ttcgatgcgg tgaacatccg cgttcagcga ttgcgccatt
     4261 tcgggaatcg cgggcaggta ggcatcgatg gaaaacggca tcagcgtaac cagcatcgcc
     4321 atcaaaaccg ccatcagttt ttcgctcatt tcaggataat gggtagaagg cataaattct
     4381 cgtccgattc gcaaacaaac cgcccggcgg cggaaaacac aatacgcgga aacggaaaat
     4441 tttaacacac cggcacaaac aggcggcaac aaaagccccg gcaaaaccgg acgaaccgga
     4501 acgatatagt ggattaacaa aaaccagtac agcgttgcct tgccttagct caaagagaac
     4561 gattctctaa ggtgct
//
LOCUS       DAWGJV010000144         4516 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWGJV010000144 DAWGJV010000000
VERSION     DAWGJV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.144"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(58..1851)
                     /locus_tag="WHQ94_001760"
     CDS             complement(58..1851)
                     /locus_tag="WHQ94_001760"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ2129000.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2097..3206
                     /gene="proB"
                     /locus_tag="WHQ94_001761"
     CDS             2097..3206
                     /gene="proB"
                     /locus_tag="WHQ94_001761"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ2129001.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3219..4481
                     /locus_tag="WHQ94_001762"
     CDS             3219..4481
                     /locus_tag="WHQ94_001762"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ2129002.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLEQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1036 a         1226 c         1254 g         1000 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaagc
      121 ggaaaggtag gctttggcag tggcaaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgtgcca gacggacgct ggtttcgcct tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgataa aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 acgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaaatctcaa taaaccgcca tagattttat taattttata
     1981 cattttgtcc attttattgc agacgcggcg tgattgttga caattgcggc agaacagggc
     2041 aaattcggcg gcacaaggtg gcaacgagac aggacaaacc aacaaggacg gaaacaatga
     2101 aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg gacggcagtc
     2161 tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat catgcgggac
     2221 acgagctggt cttggtgtct tccggcgcgg ttgcggcagg gttcggtgcg ctgggtttca
     2281 aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg caggggctgc
     2341 tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgcg caaatcctgc
     2401 tcagccgcgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc gcactttccg
     2461 tgctgctgca acgccgcgcc gtccccatca tcaatgaaaa cgatacggtt tcggttgagg
     2521 aattgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg atacaggcag
     2581 acctcttggt gctgctgacc gacatagacg gtctttacac gggcaacccg aacagcaatc
     2641 ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc gaaatggcgg
     2701 gcggatcggg ttcggcaaac ggcacgggcg gtatgctgac taaaatcaaa gcggcgacga
     2761 ttgcgaccga gtccggcgta ccggtctata tctgttcctc gctcaaaccc gatgcacttg
     2821 ccgaagcggc agataatcag gcggacggct cgtttttcgt cccccgtgcc aaaggtttgc
     2881 ggacgcagaa gcaatggctg gcgttctatt ccgaaagcag gggcggcgtt tatgtggacg
     2941 aaggtgcgga acacgctttg tccgaacagg gaaaaagcct gctgatgtcg ggcattgccg
     3001 gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag gcaaccaaac
     3061 agcctttggg caaagggcga gtcctgttcg gctctgccgc cgccgaagac ctgctcaaat
     3121 tgcgtaaggc gaaaggcgtg ttcatccatc gggacgactg gatttccatc acgcccgaaa
     3181 tacgcctgct tctgaccgaa ttttagaaaa gaaaacccat gtcaaacaca caaaaacagc
     3241 ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa gaaaaaaacc
     3301 gcgcgctgtt tgctatggcg gacagcttgg aagcggcggc ggcagatatt ttggcggcaa
     3361 accgtcaaga cttggaagcc gcggcaggca aaattcccga aagcatgact gaccgccttt
     3421 tgttggacgg caaacgcatt tgcgcgatgg cggacggcat cagggcggtt gccgcactgc
     3481 ccgaccccgt gggcgaaata ctggaaacct cgactctgcc caacggcttg gaaatcgtca
     3541 aaaagcgtgt ggcgatgggc gttatcggca ttatttacga aagccgcccg aacgttacgt
     3601 ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc agcggcaaag
     3661 atgcattcca atccgcacgc gccatcgttg ccgcactcaa agccggtttg gagcaaaccc
     3721 gcatcgaccc cgacgcgctc cagttgattg aagacaccgg gcgtgagggc agttacgaaa
     3781 tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggcg gggctgatac
     3841 gggcggtggt tgaaaatgcc gtcgtgccgg tcattgaaac ggggacgggc attgtccaca
     3901 tttatatcga caaggacgct gattgggata aggcactccg tatcgtgtac aacgccaaaa
     3961 ccagccgtcc gtccgtgtgc aactcgatgg aagtgctgct ggtacatgaa gacattgctg
     4021 ccgacttcct gcccaagctc gaacggctgt tggttcgcga ccgtatagaa gccggactgc
     4081 cgcccgtccg cttccgtttg gatccgcagg cggcgcggca tatcggcggc gaagcggcgg
     4141 gtgcagacga tttcgatacc gagtttttag actacatcct cgccgtgaaa accgtcgctt
     4201 cggtcgaaga ggcggtcggg cacatcgaaa cccacggcac gcaccattca gacggcatcg
     4261 ttaccgaaaa ccgccacgct gcggactatt tcacgaccca tatcgattct gccgccgtgt
     4321 atgtcaacgc gtccacgcgc tttaccgacg gtggcgaatt cggcttgggt tgcgaaatgg
     4381 gcatctccac gcaaaaactc cacgcccgag gcccgatggg cttgaaagag ctaacgagtt
     4441 ataaatacat cgtacaaggc acgggacagg tcagggaata atccgaattc aaacgtctga
     4501 tatagtggat taactt
//
LOCUS       DAWGJV010000145         4454 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWGJV010000145 DAWGJV010000000
VERSION     DAWGJV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.145"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(7..82)
                     /locus_tag="WHQ94_001763"
     tRNA            complement(7..82)
                     /locus_tag="WHQ94_001763"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Val,seq:tac)
     gene            complement(152..2128)
                     /locus_tag="WHQ94_001764"
     CDS             complement(152..2128)
                     /locus_tag="WHQ94_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ2129003.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2277..3116
                     /locus_tag="WHQ94_001765"
     CDS             2277..3116
                     /locus_tag="WHQ94_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ2129004.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3315..4346
                     /gene="gap"
                     /locus_tag="WHQ94_001766"
     CDS             3315..4346
                     /gene="gap"
                     /locus_tag="WHQ94_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ2129005.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FASKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1126 a         1498 c         1033 g          797 t
ORIGIN      
        1 gaaacttggt gggtgatgac ggagtcgaac cgccgacatt ctgcttgtaa ggcagacgct
       61 ctaccaactg agctaatcac cctttcgatt tgcgaaagcg ttattaaacc aaataaccgg
      121 aaatggcgca agccttattt cggcatttca tttatattcc ctgcaaactc cagttttcaa
      181 aagagataat ttcttctttt ttgcctgcga ccaccaacac gtcaccgcct tccaatgtaa
      241 acgaggcatc cgggttttca atccggccgg tgcggcggcg gacgaacaag agtttgatgc
      301 cgtaagccgc catcggaagc gtgccgactg ttttgccgac ggcgtatgct tctgcagcca
      361 aaggaaaggc gtggcggacg gtttcgccat tttcgccgaa tcctgcctca tcatcactac
      421 cgacaaacag tccctccaac gcggcataac ggctgtggcg gatatttgcc atcgtctgat
      481 agacgtgccg atacgacgcg ccgttgccta acatcgcata gcctgccagc acgagtccgg
      541 tttctttggt gtccgacacg gcttcttccg cacctatatc ggtaaacgtt ttcacataat
      601 cgtcgttggt ggcgcgcaca tatacgggca tattgggata catggacagc acattgtcta
      661 aaacgtgttg cgtttcgtgc atattgttga gcgtaaccac caccattttc gcccgtccca
      721 gaccggcggc ttccaatact tccctgcgtt tcgcatcgcc gaacgacacc ggttcgcccg
      781 cacttctggc aacctgcacc cgcgcaatgt ccaagtcgag cgcgaaatac ggaatatcct
      841 cttgggcaag gacgcgtccg accgtctgcc cgcccctgcc gaagccgaca atcagcacat
      901 ggtcggactt gctcatggtt tctaccagca tactgtgcag atcgagcgac ttcatgtccc
      961 agcttgactt gaccaaacgc ccgaccagcg catcgctgcc gcccaagagg aagggcgcga
     1021 taatcatcga cagcagcacc gccgccgtcg ccgcctgttc ccattctggc gaaaccatat
     1081 caagctgccc ggcaatggcc agcatcacga agccgaactc gccgccctgc gcgagataca
     1141 aagccgtttt gaggctgtcg ccgaccgaat gtttcatttt gaaggcaatg gcaaacacaa
     1201 ccagtgcctt caacaccagc agcattgcca acagcatcaa tacctgccgc cagccgccga
     1261 tcaatgcctg aatgtccagc ttcatgccga ccgtgataaa gaaaaagccg agcaagatat
     1321 cgcggaacgg gcggatgtcg tcttcgactt ggaaacggta ttccgtttcc gaaagcagca
     1381 tccccgccac aaacgcgccc aacgccatag acaaaccttc cagctcagtc agataagcca
     1441 cacccaaggt taccagcagc acattgatca taaagagttc ggacgatttg cgttttgcca
     1501 ccatcctgaa ccatcgcgac ataattttgc tgccgacgaa aaacagcagc cccagcgtca
     1561 gcagcatttt tgcaaacgcc aaacccaagg ccgcccaaat atttccgtcc cctccgcccg
     1621 ccagcgcggg aatcagaatc atcagcggca cgacggcgat gtcctgcatc agcagcacgc
     1681 ccatcgccat ctgaccgtgc ggctgcccca attccgtctt ttccgacaaa atccggctca
     1741 caatcgccgt ggacgacatc gccaacgcgc ccgacacggc aaacgcccaa ttgaacggca
     1801 cgcccgtcag catcagtatg cccattaccg acagcatcgt aacgccgacc tgcaaaccgc
     1861 ccagcccgaa caccagccgc ctcatcgccc tcaacttggg cagcgagaac tccaaaccga
     1921 tgctgaacat caggaacaca atcccgattt cacccaaata atccgtcgca tggcttttcg
     1981 gaatcaggct gagcataccg ggccccgcca aaaagcccac cagcaggtag cccagcatgg
     2041 agggaatgtt gaacttgcgg cacaggatca ccgtaatgac cgacaccagc aaaacaacca
     2101 caataggggc aagcgaaaat tcgttcatag accgtccgaa caggaaaata cagaaaaatg
     2161 ccgtctgaaa cggcatacgc cgccgcatta taacaaaaca ccgcgcacca tccgaaacgg
     2221 gcgcggcata caattctgct aaaatacgcc ctttcgattt tgagccgcac accgacatga
     2281 ccgccacagc cgccgccata gaccgcctgc ttccccaaac ccaatgccgc gaatgcggct
     2341 acgacggctg cctgccctac gcacaggcag tcgcaacagg cgaagcgtac aacctctgcg
     2401 ccccgggcgg agaagccgtc gtccgcgacc ttgccgccct gctcggcaaa ccccttgtcg
     2461 cacctgccaa aacccaagcc aaagcactcg cccggataga cgaaaccgcc tgtatcggct
     2521 gcaccgcctg catccgcgcc tgccctgccg atgccattat gggcgcgggc aaacttatgc
     2581 acaccgtcat cgccgacgaa tgcaccggct gcggactctg cgtcgccccc tgccccgtcg
     2641 actgcatcca tatgcagccc gttgccgaca ccgtcctgcc ccgcgcgcgc cgcttcagcc
     2701 tgtccgccga cagccgtttt gccgccgccg aacacgcgcg cacgcgctac ctcaaacgca
     2761 acgaacgcaa acagcgcgaa gccgacgaac gcaaggccat gcttgccgaa cgcgaagccg
     2821 ccgtccgcaa cgcgcgtccg caaacgcccg acacaccgaa aaaaccggcg tttaaccctg
     2881 ccgacctcat cgccaaagcc atggcaaaag cgcaaaccca acaagaccgc ctcgccgccg
     2941 ccgacaaccg caaagactat caggcgaaac agatagccga agcccgcgaa cgcgccgagt
     3001 tgcgccgcgc ccaacgcgat atgaaatacg gcagcgacag cgaaaaagcc gccgccctcg
     3061 aatacctcaa acaatacaaa gccaaacagg aagccgcaca gaataccgcc tcctgaccct
     3121 tccctgatat gccgtctgaa gccgcttcag acggcatttt atcaagctct ccgtccgccg
     3181 cacccgtgcc gtccgcatct taccgcccac ccttccggcc gcgccgtttt caataaaata
     3241 ttaattacac gccactacaa atttgctata atccgccccg aaaatctacc caaccctcaa
     3301 caaaggaacg aaccatgggc atcaaagtcg ccatcaacgg ctacggacgc atcggccgcc
     3361 aggttttgcg cgccatctac gactatcaga ttcaagacca actccaaatc gtcgccgtca
     3421 acgccagcgg cagccttgaa accaacgccc atctcaccaa attcgacacc gtgcacggac
     3481 gctttgaagc cgacgtatcc cacgacggcg gcaacctcat cgtcaacggc gacaaaatcc
     3541 ccttcttctc gacccgcaac cctgccgaac tgccgtggaa agaactcggt gtcgatttgg
     3601 tcatggaatg caccggcgcg ttcgccagca aagaaaaagc caaagttcat ctggaaagcg
     3661 gcgcgaaaaa agtcctcatt tccgcaccgg gcggcgacga tgtcgatgca accgtcgtgt
     3721 acggcgtgaa cgacagcatc ctgaccgccg acatgaccgt cgtttccaac gcttcctgca
     3781 ccaccaactg cctctcgccg gttgccaaag tgttgagcga aagcgtcggc atcgtcaaag
     3841 gcgcgatgac caccatccac gcgctgacca acgaccaaac cgttaccgac gtgcgccaca
     3901 aagacctgcg ccgcgcccgc agcggcgtgg aaaacatgat tccgaccaaa accggcgcgg
     3961 caaaagccgt cggtttggta ctgcccgaat tgaaaggcaa actcgacggg cttgccatcc
     4021 gcgtgccgac cgtcaacgta tcattggtag atttaagctt ccaagccgcg cgcgacacga
     4081 cagtcgaaga aatcaacgca ctgatgaaag ccgcctcgga agcaggcgcg ctcaaaggcg
     4141 ttttaggcta caacaccctg cccttggttt ccatggactt caaccacacc accgaagcca
     4201 gccacttcga cgcaacactg accaaagtcg ttgacggcaa catgattaaa gtcttcgcat
     4261 ggtatgacaa cgaatggggc ttcagctgcc aaatgctgaa caccgcacgc cgtatgttcg
     4321 gacttgaagt gcgcccgctc aaataagcaa taaaccgtca aacaaaatgc cgtctgaaac
     4381 ccgatgtttt caagttccag acggcatttt tcatttttca ccgtgatttt acccggctgt
     4441 cgtcatttct aatt
//
LOCUS       DAWGJV010000146         4384 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWGJV010000146 DAWGJV010000000
VERSION     DAWGJV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.146"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(44..3076)
                     /locus_tag="WHQ94_001767"
     CDS             complement(44..3076)
                     /locus_tag="WHQ94_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ2129006.1"
                     /translation="MFWIVLIVILLLALVGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     HADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAAPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETGAVSEHLPITDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3241..3921
                     /locus_tag="WHQ94_001768"
     CDS             3241..3921
                     /locus_tag="WHQ94_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ2129007.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASADGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3950..4309
                     /locus_tag="WHQ94_001769"
     CDS             3950..4309
                     /locus_tag="WHQ94_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ2129008.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          891 a         1285 c         1118 g         1090 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatgcg caaggcgcgc tgtacgcccg
      181 aaatactggc tttgcgcgtt ttcaggacaa cggatacggc ttcatcatac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gcttcagata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggccgtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggatact tgaaacgcga tacgcgtcgg gatgtttgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgcg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcaacc acgaccacga taaacggcaa tttttccaaa ggttcgggat
      721 tgtcgggcgt gaggctgaac gggttgccga ttttctcccc ccttgctgcg gcttcggcga
      781 ttttttgatt gaaacccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggtgctttgc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttatcggcag gtgttcggat accgcaccgg tttcttccga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacatcttca aacggacaaa
     1621 tcgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ctcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggtct cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacagtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcaggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgtccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctgcatgacg gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag
     2281 acggatgctg cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt
     2341 ctttgtccgc atcgaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga
     2401 taatctgcag cccttccatc ggggtatcgg acacgcggac tttgggggcc gctgtttcgt
     2461 ttttctcaaa tgcatcgata tagcgttttt tcgtttccct taaagccgcg ctgtttaaag
     2521 cacgcgttgc ttcttccaaa gtgatttctt taaaaaccgg acggacgggc gaaatttcgc
     2581 tttcagacgg catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga
     2641 ttacggcagt tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgctgctt
     2701 ccgtttccgc ttcttccgtc ccgtttccac tgtctgaata cccgtcggat gcgttcggaa
     2761 catctgcatg gtttgcagaa gcaggcggaa cgggcctgtc cggttcggat tcgtgcgcca
     2821 gatagtttcg gcagaaacgg acgacaccga caaacagcca atacactgcc gttttgacgg
     2881 catggaaaag catcagggca aactcgggaa aatcgggcat accgtctttg atttcaggca
     2941 gctccgcctg tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc
     3001 gttcggattg tgcgcggaca aaaaacaagc cgacaagcgc aagcaacaaa ataacgatca
     3061 aaactatcca aaacatatgc cgtttcccgc aagacaaacg attaaacggg tttattttaa
     3121 cgtgtttcca tcccaaaaaa aacaaccggc cggcaattct gccgaaaccg attcggcttt
     3181 cggtttgccc cagccgacag tataatagcc gtcccttccc ttaatctttg cggcattgcc
     3241 atgattttcc aaacagtctg gttttcagat atggtactgt cggtttcgtg gattgtcctt
     3301 atcctgatac tggcagcttc cgcgccgtcg gcattccgct cgcttgcccg ctaccgttcc
     3361 gcccttcccc tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgccgac
     3421 ggcggacagc ttgcccagat gaactaccat ctgctcgccg tcaatctggt tgcactgatg
     3481 gtgggcactt ccgccgcctt ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg
     3541 tttgccggtt ctgccggcgc atatccgccg aacgctttgg tgctgattct gcccgcgctt
     3601 gccgtcaacc gcctgtcgcg tatgctggtc aaccgcctgc cgcccaatat tttcatcttt
     3661 attttcgtca acggctttct cgcttccgct gccggcattc tgttgaccgg gctggtgctg
     3721 accggtattt tggatgctgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg
     3781 cccgtcttta ttctgctggc gtgggcggaa gccttcctca gcggcatttc aactgccata
     3841 tttgttgcac tgaagcccca ttggatcaac actttcgacg acaaccgcta tctgaagtcc
     3901 gaccgcggca tatggcggta gccttcagac ggcataggca gacaaaacca tgctttccaa
     3961 catcatcccc ctttccatcg gtgcggcact cggtgcaacg gcgcgttggc tgctcaacct
     4021 cgccgttcct gccgccatgt ctcccgccac gggcaacctg tttgccaact ggacgggcgc
     4081 gctgctcatc ggcatctttg ccgaaaccat caatcacccg caatggaagc tgctgctgat
     4141 taccggtttt ctcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct
     4201 gctccaatcg aatcgtcctg cttcagcact tgccaatatt ttcctacaca cggcaggttc
     4261 gctgctgctg acttggctcg ggctgaagat aggggcggca gtcaaataac cgcaacaaat
     4321 tgcctccctt tgttttgaca taatctataa attagcttcg cttgtttaaa tactggctgt
     4381 gcca
//
LOCUS       DAWGJV010000147         4370 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWGJV010000147 DAWGJV010000000
VERSION     DAWGJV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.147"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            266..1186
                     /gene="ribF"
                     /locus_tag="WHQ94_001770"
     CDS             266..1186
                     /gene="ribF"
                     /locus_tag="WHQ94_001770"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ2129009.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRCGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            1327..4116
                     /gene="ileS"
                     /locus_tag="WHQ94_001771"
     CDS             1327..4116
                     /gene="ileS"
                     /locus_tag="WHQ94_001771"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ2129010.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LATAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPTGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1061 a         1197 c         1168 g          944 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gggttgtttg tctcggttta cctggttaaa aaagaacgat
       61 tttcactgat gttgcatcag gtttggggcg atgtttcaac acatagcacc gcgcctgctg
      121 cgcgctttat gcgtttggcg cgttcggctg cgggaaattt gcctactttt cccgcgtcgg
      181 gcgggcgtaa cgggcggcac actgtctata aaccgcaata ccgtttacaa tgaccgcctg
      241 tttcaccaca tacccgaaca taacaatgag aatcaggctg gggcggcaca acgcgcccga
      301 ctttccgcac ggggccgccg taaccatagg caattttgac ggcgtacacc tcggacacaa
      361 acacatcctc caaaaactcc gcctcgaagc cgatacgcgc ggattacccg tcgtgaccgt
      421 cgttttcgaa ccccaaccca aagaattttt cgcactccgc accggcagga tgccaccgtg
      481 tcggatcagc cccctgcgca ccaagctcga attattggaa ggcacaggct gtgtcgatgc
      541 cgtctgggtt ttgcgtttcg atcaaaattt ttccgaaata tccgcgcaag ggtttatcga
      601 ccgcctgctg cgtcaaacct tgaatacgcg ttatttgctc gtcggcgatg atttccgttt
      661 cggtgcgggg cgggaaggct gttttgaact tttggcacaa cagcccgata tgcagaccga
      721 gcgtacgcct tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg tgcgacaagc
      781 cctttcagac ggcaaccttg cctatgcgaa aaaacttttg ggacacgact acgtcttgag
      841 cggcagggtg gtgcacggca gaaaactcgg acgcacctta aacgccccga ctgccaacat
      901 ccgcctgccc cgccaccgtt atgcactcgg cggcgtgttc gtcgtcgaag cggacggcgc
      961 attcggcacg cggtgcggcg tggcgagctt cggtttcaat cccaccgttg atagcggctg
     1021 ttctcaaaag cttgaagtcc acctgttcga ctttcaaggc gacctgtacg gacaacggct
     1081 gaacgtccgc ttcctgcaca aactgcgcga tgaagaaaag tttgatggta tggcagaact
     1141 gaaaaggcag atagcagccg atatggaagc cgcaaagtgt tggtagaaaa accttataca
     1201 aaccatccga ttgggctaca atcaatcttt taacttttca gacggcatag gggtttcccg
     1261 ttgtgaaata ctgtttgagg cgcaatgccg tctgaaccga aatattgtaa caatagagat
     1321 taaaaaatga ccgattacag caaaaccgtc aacctgctcg aaagcccgtt cccgatgcgc
     1381 ggcaatcttg ccaagcgcga gcctgcatgg ctgaaaagct ggtacgagca aaaacgctac
     1441 caaaaactgc gcgaaatcgc caaaggccgt ccgaaattca tcctgcacga cggcccgccg
     1501 tatgccaacg gcgacatcca catcggtcat gccgtcaata aaattctcaa agacatcatt
     1561 atccgcagca aaacccaagc cggttttgac gcgccttatg tgccgggttg ggactgccac
     1621 ggtttgccca tcgaagtgat ggtagaaaaa ctgcacggca aagacatgcc taaagcgcgt
     1681 ttccgtgaat tgtgccgcga atatgccgcc gaacaagttg cccgccagaa aaaagacttt
     1741 atccgcttgg gcgtgttggg cgactgggac catccttacc tgactatgga tttcaaaacc
     1801 gaagccgata ccgtgcgtat gctcggcgaa atctacaaat ccggctatct ctaccggggt
     1861 gcgaaaccgg ttcaattctg cttggactgc ggttcttcgc tggccgaagc ggaagtggaa
     1921 tacaaagaca aagtatcgcc tgcgattgac gtgggctatc cgtttaaaga caccgctgcg
     1981 cttgccactg cattcggttt ggcaggtatc gaaggcaaag cgtttgccgt tatctggaca
     2041 accacgcctt ggacgctgcc ggcgagccaa gccgtgtctg ccggtgcgga cgtggtgtat
     2101 caattaatcg atacgcctac aggcaaattg gtgctggcga aagatttggc agaagacgcg
     2161 ctcaaacgtt acggtttttc agacggcatt gctattcttg ccgaaaccac cggcgacaag
     2221 ctggaaaacc tgcacatgaa tcatccgttc ctcgagcgtg atattcccat gctcaacggc
     2281 gaacacgtta ccaccgacgc cggtacgggt ttggtacata ccgcccctgc gcatggtttg
     2341 gaagactacg ctgtctgcaa taaatatggc atcgagcttt acaaccccgt caacgccgaa
     2401 ggcaaataca tcagcgaaac gccgcgcgtt gccggtatgc gcgtttggga agccaatccc
     2461 gttatcctgc aatggctgga agaaaccggc aaccttttgg caagcagtaa aatcgaacac
     2521 agctacgccc actgctggcg gcacaaaacg ccgctgattt accgcgcgac cggtcaatgg
     2581 tttgtcggta tggacaaagc cggtgccgac ggcaaaaccc tgcgcgacaa agccatcaag
     2641 gccgtggacg ataccgaatt cttcccgtct tggggtcgcg cgcgtttgga agccatgatt
     2701 gaaggtcgtc ctgactgggt ggtttcacgc caacgctatt ggggcacgcc gatgactttc
     2761 tttgttcaca aagaaacggg cgagctgcat ccgaactctg ccgaactttt ggaaaaagtc
     2821 gcgcaacgca ttgaagaaaa aggcatcgag gcttggttct ccctcgataa aagcgaatta
     2881 ttaagcgcgg aagattgcga acattacgac aaactccccg atacgatgga cgtatggttc
     2941 gattccggtt cgacccatta ttccgttgtg aaacaacgcg aagaattgga atggccggcg
     3001 gatttgtatc tcgaaggcag cgaccagcac cggggctggt ttcaatcgtc catgctgacc
     3061 ggctgcgcct catcaatggg acgcgcaccg tataaacagc tgctgaccca cggttttgtt
     3121 gtagacggcg aaggcaaaaa aatgtcgaaa tccatcggca acgtcgtcgc gccgcaagaa
     3181 gtctataacg aattcggcgc ggacatcctg cgtctgtggg cggcatctac cgattacagc
     3241 ggcgaattgg cgatttctaa agaaatcctt aagcgcgtaa ccgaaagcta ccgccgtatc
     3301 cgcaatacct tgagcttttt gtttgccaac ttgagcgact ttaatccgat tgaagatgcc
     3361 gtgcaacagg cggatatggt ggaaatcgac cgctatgccc tggtgttggc gcgtcggctg
     3421 caagagcgtc tggcaggcga ttactatccg cgttatgcct tccattttgc cgtgaaagac
     3481 attgtttctt tctgctcgga agacttgggc gcgttctacc tcgatatcct gaaagaccgc
     3541 ctctacacca ccaaagccga cagccgcgcc cgccgcagcg cgcaaaccgc cctgtaccac
     3601 atcacgcgca gcctggttct cttgattgca ccgattttgt gctttaccgg cgaagaagcg
     3661 tgggacatca tcggcggcgg cgaagaagac agcgtcctct tccatacttg gcacgagttc
     3721 ccgaccatca acgaaaaaac cgaagccgaa ctggtgaaaa aatggacggc aatccgcgaa
     3781 gcccgcgaag cggtaaccgc cgccatcgag cctttgcgcg ccgacaaaac cgtcggttcg
     3841 tccttgcaag ccgaagccga aatcaccgcg ccggaagaaa tggccggcta cctgaacgcc
     3901 ttgggcgaag aattgcgctt tgccatgctg gtgtctaaag cagaagtgaa agtaggcagc
     3961 gaacttgccg ttgccgctaa agccagtgat ggtgaaaaat gcgaacgctg ctggcactac
     4021 acccgcgatg tgggcgcggt tgcaggctat gaaaccgtct gcaaacgctg tgcagagaat
     4081 gtcggcggag aaggcgaaac gcgccattac gcctgataaa gtttgagcaa atgccgtctg
     4141 aaaccgccga acagcatttc agacggcatt ttttgtgccg caatttgtct tcataatggc
     4201 ggacgggttt caagattacg gtgttgtcgg aatgcaactg aaccgtcatt cccacgaaag
     4261 tgggaatcta gaatctcgag gtttcagtca tttccgatag attcccgccg cgtcgtgggt
     4321 ctggattccc gcctgtgcgg gaatgacgga tttcgagatt gcggtgttgt
//
LOCUS       DAWGJV010000148         4342 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWGJV010000148 DAWGJV010000000
VERSION     DAWGJV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.148"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..281)
                     /locus_tag="WHQ94_001772"
     CDS             complement(<1..281)
                     /locus_tag="WHQ94_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ2129011.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYSGLTKTSTA"
     gene            complement(278..424)
                     /locus_tag="WHQ94_001773"
     CDS             complement(278..424)
                     /locus_tag="WHQ94_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129012.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(441..779)
                     /gene="erpA"
                     /locus_tag="WHQ94_001774"
     CDS             complement(441..779)
                     /gene="erpA"
                     /locus_tag="WHQ94_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ2129013.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(911..1597)
                     /locus_tag="WHQ94_001775"
     CDS             complement(911..1597)
                     /locus_tag="WHQ94_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2129014.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYITRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            1783..2055
                     /locus_tag="WHQ94_001776"
     CDS             1783..2055
                     /locus_tag="WHQ94_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129015.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2263..4191
                     /gene="dnaK"
                     /locus_tag="WHQ94_001777"
     CDS             2263..4191
                     /gene="dnaK"
                     /locus_tag="WHQ94_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ2129016.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1330 a         1135 c          870 g         1007 t
ORIGIN      
        1 aacgccgtac tggtttttgt taatccacta tatctccggg taataatatc caaacccaaa
       61 tagctgaaat aatcgtgcaa ctcttcccgt ttcgcccaag tgcatttttc ctcaaccagc
      121 acttgaggtt tggaatcgag cgattcgatc aatccttccg ccttttcctc gccgagattc
      181 tccctgaggc actcccggat aagcccgcga tccacttggg gcgggtaaga aacatataca
      241 tcgtacaaat cgtttcggta atctttactc atctcaatca taagggaaca aatacgcctg
      301 ataccgttcc gcccgttcct ttcgggaaac ccgattcatc ttcaaacaat cggattatat
      361 gtgaattcat cccgataata tattttccaa aagtaaaaat gccgtctgaa cgttcagacg
      421 gcatcacaca aaccaagcgg tcatacggaa aacgacgatc cgcaaccgca ggttgtttcc
      481 gcattcggat tgcggatgac gaattgcgaa ccctgcaaac tttccgtata gtcgatttcc
      541 gcaccgacca gatattgata gctcatcgga tcgaccaaaa agaccaaacc gtttttctca
      601 atttcaaaat cgtcgtcgtt tttgatttcg tcaaaagtaa atccgtactg gaaacccgaa
      661 cagccgccgc cattgacaaa aacccgcaat ttcaaatcgg gattgttttc ttcggcaatc
      721 aaatcggcaa ctttggcaca gcagctgtca gtaaaaataa tagggctttc atccgacatg
      781 gtggtattcc ttaatataat attttctgca ttctctctca aacccccttc aaaggcaagc
      841 aggcttcgcc tttgaaaaag gaattgttcc aatagataag tttgaccgcc tgcaatttca
      901 agccccaaac tcaaatcgtc ctgccgaacc actcgacacg cccgataatc tccacatcat
      961 cggtcaaatc gttcaaattg atttcaaaag caggataagc ctcgtttgca gaaattacat
     1021 tgataatccc gcccggcaca atctgtaaac gtttaaccag cagattttca ttaatccgca
     1081 acacatacag accgtccctc ggcgtatttt ccccatgatt gaccaaaatc gaatcgccgt
     1141 cattcaaaac cccctccatc gaatccccct tgacggaaat tacagacagg tttttcgtat
     1201 cgcgggtaat ataattctca atccaatgcc gtctgaacgc cattgtaaat accggttcct
     1261 catgatcgac aaactgcccg tatcccgcag ccgcccgaat atcatatctc ggcacaaaga
     1321 caaactcgtc cgtatcaact tcattgccca aggtatcgta agcaagggat ttttttgggg
     1381 cttcatccgg aaacggatta ccctccccgg tcagcagcca atcgatacta caccccttca
     1441 actgcttgat ttttttcaat gtttcagact tcggcagacc gccttcattc catatcctgc
     1501 tgaagcccgc aatcgtcatt tcaatatcgg atgcgatttt tgcctgcttc gcttcgcttt
     1561 tccataaaaa aaccagtctg tctttaaagg tttccataaa tatccttccg ctccccttca
     1621 ttcaatcatc taaagtaaac atgaaatata ttttacctta gctatcccaa aaacggaaca
     1681 actatgataa actaaattta aattatgatt tttcttacta aatacataat ttttaagatt
     1741 ttccataggt ttaatcctga attgacagaa aaaggataga aaatgaagaa aaccggcaaa
     1801 tatcttatct atgctgcggc atttaccgca ttctgctttg ccttccaaga aaaccgttct
     1861 gaagccaaac agcctgacat cactttatcc gcatccctgt gcgaacaatt caacatactg
     1921 aacgccaaag atatggatgc agaacaagtc tccctttcca aagaatgcga catcatcgag
     1981 tcttcacacg actgggaaaa agagtacggc aacttgaacg aacaggaaat gctcgccggc
     2041 atcgtctatg aataaacctg cctgccattt gaaacattat gcttgaatgc attggagcca
     2101 aatgtattaa atcaaatata aaaccaatat attcataaag ttatatactt atagccatgc
     2161 tgtagcttga aacagcccgc cgcccgccct atttacagcc catcgggaca aaatgttcca
     2221 acatatttct caaaataagc aaaatcaaat aggattatcc acatggcaaa agtaatcggt
     2281 atcgacttag gtacaaccaa ctcttgtttg gccatttccg aaaacggtca aaccaaagtt
     2341 atcgaaaacg cagaaggcgc acgcaccacg ccgtccgtta tcgcttattt ggacggcggc
     2401 gaaatcctcg tcggcgcgcc tgccaaacgc caagccgtaa ccaacgctaa aaacactatt
     2461 tacgccgcca aacgtttgat cggccacaaa tttgaagaca aagaagtcca acgcgacatc
     2521 gaatctatgc cttttgaaat catcaaagcc aacaacggcg acgcatgggt gaaagcgcaa
     2581 ggcaaagagc tgtctcctcc tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc
     2641 gctgaagctt acttgggcga gaaagtcacc gaagccgtga ttaccgttcc tgcctacttc
     2701 aacgacagcc aacgtcaagc aactaaagac gcaggccgca tcgcaggttt ggaagtaaaa
     2761 cgtatcatca acgaaccaac cgcagccgct ttggcattcg ggatggacaa aggcgacaac
     2821 aaagaccgca aagtagccgt atatgacttg ggtggcggta ccttcgatat ctccatcatc
     2881 gaaattgcca acctcgacgg cgacaaacaa ttcgaagtat tggcaaccaa cggcgatact
     2941 ttcttgggcg gtgaagactt cgaccaacgc ttgatcgact acatcattga cgagttcaaa
     3001 aaagaacaag gcattgattt gaaacaagac gtaatggctc tgcaacgtct gaaagaagct
     3061 gctgagaaag ccaaaatcga attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac
     3121 atcactatgg acgcaaccgg tccgaaacac ttggcaatga aaattacccg tgccaaattc
     3181 gaaagcctgg ttgaagactt gattgcccgc tctatcgaac cttgcaaaat tgcattgaaa
     3241 gatgccggct tgagcaccgg cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt
     3301 atgccgaaag tacaagaagc cgttaaagac ttcttcggca aagagcctcg taaagacgtg
     3361 aaccctgatg aagccgttgc cgtaggcgca gcgattcaag gcgaagtatt gagcggtggc
     3421 cgcagcgacg tattgctgct ggacgtaacc cctctgtcct tgggtatcga aaccatgggc
     3481 ggcgtgatga ccaagctgat tcaaaagaac acgaccattc cgactaaagc gtctcaagtg
     3541 ttctctactg ccgaagacaa ccaaagcgca gtaaccatcc atgtactgca aggcgaacgc
     3601 gaacgcgctt ctgccaacaa atctttgggt cagttcaact tgggcgacat cgcacccgca
     3661 ccgcgcggta tgccgcaaat cgaagtaacc ttcgacatcg acgccaacgg catcctgcac
     3721 gtttccgcca aagacaaagg caccggtaaa gcggccaaca tcaccatcca aggttcttca
     3781 ggcttgagcg aagaagaaat cgaacgcatg gtgaaagatg ccgaagccaa tgctgaggaa
     3841 gataaaaagc tgactgaatt ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc
     3901 gtgaaaaaat ctttggccga ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc
     3961 gaagccgcgc tgaaagaagc cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat
     4021 gccaaagccg aagcactggg cacagccagc caaaaactgg gggaaatggt ttacgctcaa
     4081 gcacaagctg aagcccaagc aggcgaaagc gaacaagcca atgcttctgc aaagaaagac
     4141 gatgatgtcg tagatgccga ctttgaagaa gtaaaagacg acaaaaaata attgatgccg
     4201 tctgaaaaaa gcgcgaaccg tttgattcgc gcttttttca attgagataa aagaccatag
     4261 cataacagag gttccaaatt catctaccgt gatattccca agcccaagtt ctaactgtta
     4321 tatcggttct ctggaaattt tt
//
LOCUS       DAWGJV010000149         4261 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWGJV010000149 DAWGJV010000000
VERSION     DAWGJV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.149"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            538..2265
                     /gene="ilvB"
                     /locus_tag="WHQ94_001778"
     CDS             538..2265
                     /gene="ilvB"
                     /locus_tag="WHQ94_001778"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ2129017.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2276..2767
                     /gene="ilvN"
                     /locus_tag="WHQ94_001779"
     CDS             2276..2767
                     /gene="ilvN"
                     /locus_tag="WHQ94_001779"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ2129018.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2834..3127
                     /locus_tag="WHQ94_001780"
     CDS             2834..3127
                     /locus_tag="WHQ94_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ2129019.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3207..4220
                     /gene="ilvC"
                     /locus_tag="WHQ94_001781"
     CDS             3207..4220
                     /gene="ilvC"
                     /locus_tag="WHQ94_001781"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ2129020.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1106 a         1023 c         1077 g         1055 t
ORIGIN      
        1 atcaatatat taagtcgttg ttagccctct tggttctgtc aatgccgtcc aatacagtcc
       61 ggtgcaatga gccattttca ggaagtatgg ttgggaatat ttttgatata tcaaaaataa
      121 aatcaagttc aatcaatatc ccgagtcttt attgggatga aaccctgtaa attttacaat
      181 tcttgatatc gaagaagggt ttttcacatc tgcccctcct acacaaactg tcaacaattt
      241 ccatctttca aaaaaggtac tcctgatttt attcttttaa aatcaatcaa taatttctct
      301 gctttcaaaa aatattaaaa gatgaaagat ttttcttgat tgaactgtcg gtttgcgtta
      361 tcgtttctac tctttttcac aaactctgtg ttcctttcca attgattgga taatccgtct
      421 gttgccgtgt tcccccaatg cgcgtaccct aaatcgctgc ttggtggaat tgcattacag
      481 gtgctgtggc aaagcggttt gtccctattg gtttgaaacc gtataaaaga ggtcgttatg
      541 cagttatcag gcgcgcaaat catagtgcag agtctcaaag ccgaaggtgt cgagtacgtt
      601 ttcggttatc ccggcggtgc ggttatcgaa atctacgatg cccttttcca actcaataaa
      661 ttcaagcaca ttctgacccg tcacgagcag gcggcagtac acgcggcaga tgcgtatgcg
      721 cgcgtcagcg gtaaggtggg cgtggcattg gttacatccg gcccgggcgt taccaatgca
      781 ctgaccggta ttgctactgc ctatacggat tcgattccga tggtggtcat cagcgggcag
      841 gtaggcaatt ccctgattgg tacggatgcg ttccaagaag ttgatacggt gggtattacc
      901 cgtccgtgcg tcaaacacaa tttcctggtt acggacatca atgagttggc ggaaaccatt
      961 aaaaaggcgt tccaaattgc cgcaagcggc cgaccggggc ccgtggtggt tgatgtcccg
     1021 aaagatgtta cgcaggcgat ggcgaaattc agctatcctc aggaagacat ttttatccgt
     1081 tcgtatcaac cggttgttca agggcatatc ggtcagatta aaaaggccgt gcagatgttg
     1141 gcatctgcca aacgtccggt cgtctatttt ggcggcggcg tggtgttggg taatgcttct
     1201 gaagagctga cccgatttgt ccgaatgacg ggtgctccgt gcacgggtac gctgatgggc
     1261 ttgggcgctt atccttccgg cgaccgccaa ttcctcggta tgctcggtat gcacggtact
     1321 tacgaggcaa accttgccat gcagaatgcg gatgtcgttc ttgccgtagg tgcgcgtttt
     1381 gacgaccgtg tggtttccgt accgtccaaa tttttcgaga aggcgaagaa ggttatccat
     1441 atcgatgtcg atccttccag catcgccaaa cgcgtgaagg cggacattcc gattgtcggc
     1501 gacgtgaaaa acgttttgtc cgagatggtt gcgctgtggc aaaaacaaga gtccgtgccg
     1561 tctgaagatg ctttgggcaa atggtggaaa accatagagg aatggcgttc ccgcgattgc
     1621 ttgtggtttg acaacggcag cgaaattatc aagccacaat atgtgattca gaagcttgcc
     1681 gagattaccg gcaattcggc aatcatcaca tcggatgtag ggcagcatca aatgtttgcg
     1741 gctcaatatt atcccttcga acgtccgcgc caatggctca actccggcgg tttgggtacg
     1801 atgggcgttg gtctgcctta tgcgattggt gcaaaacttg ccgccccgga tcaagacgta
     1861 ttctgtatta ccggcgacgg ctcgatccag atgaacatcc aagagctgtc cacctgtttc
     1921 caatatcgga ttccggttaa cgtcattacg ctgaacaacg gttatctcgg tatggtacgc
     1981 cagtggcagg aaatatatta cggcggccga gagtcggaaa cttatttcga ttctttgccc
     2041 gatttcgtca aacttgccga ggcatacggt catatcggca tccgcgtgga caagaagtct
     2101 gatgtggaag gtgcgttgtt ggaagcattg aaccaaaaag acaggctggt gtttatcgac
     2161 ttcctgaccg accagaaaca gaatgtgatg cccatggtcg gtaacggcaa aggtttggac
     2221 gaaatggtac ttccgccgca tatgcgtgcg gacgaaaagg cgtaaggaga ggcaaatgcg
     2281 acatatctta tctgttctga ttgaaaacga atcaggtgcg atgagccgcg tggtcggttt
     2341 gttctctgca cgcgattaca atatcgattc tttggcggtt gcgccgaccg aagacaaaac
     2401 cctgtcccgt atgaccatcg ttacccacgg cgacgagcag gtcatcgaac aaatcaccaa
     2461 gcaactcaat aaattgattg aagtgattaa agtggtcgat ttgaacgaaa gccgttttgt
     2521 cgaacgcgaa ctgatgttgg taaaagtccg tgccgccggc aaagaccgcg acgaattttt
     2581 acgcttgacc gaaatctacc ggggcagcat catcgacgta accgaccgca gctatacgat
     2641 tgaaattacc ggctcgacag acaagctgga ttcctttttg gaaacggtcg gacgcgccca
     2701 aattttggaa accgtacgca caggcgcggc cggcatcggt cgcggtgagc gtattttgaa
     2761 aatttaacgc cgtaaccttt cagacggcac ggtatttgaa tgtcgtctga aaaacgaacg
     2821 gcaggagaga tttatgtcga acattaaaat tgtcgcactg gttaccgtca aaccggaata
     2881 cacagaaaca ctggcagcac agtttaaaga actggtcaaa gccagccgtg cagaagaggg
     2941 caacatcagc tacgatctcc atcaggaaat cggcaaaccg aaccgttttg ttttcgtgga
     3001 aaattggaaa tctcaagcag ctattgacga acataacgcc agcgcgcatt tccaagcctt
     3061 cgtccagtcc gtcgacggca aaaccgaagc gttggaaatc gtattgatga atgaagttgc
     3121 cgattaagcg gcacactctg tttaacccgt ccgaagccgc tcaacatttt aaggcttcga
     3181 caaccattta ccttaaagga aatcaaatgc aagtctatta cgataaagat gccgatctgt
     3241 ccctaatcaa aggcaaaacc gttgccatca tcggttacgg ttcgcaaggt catgcccatg
     3301 ccgccaacct gaaagattcg ggtgtaaacg tggtgattgg tctgcgccaa ggttcttctt
     3361 ggaaaaaagc cgaagcagcc ggccatgtcg tcaaaaccgt tgctgaagcg accaaagaag
     3421 ccgatgtcgt tatgctgctg ctgcctgacg aaaccatgcc tgccgtctat cacgccgaag
     3481 ttgcagccaa tttgaaagaa ggcgcaacgc tggcatttgc acacggcttc aacgtgcact
     3541 acaaccaaat cgttccgcgt gccgacttgg acgtgattat ggttgccccc aaaggtccgg
     3601 gccataccgt acgcagtgaa tacaaacgcg gtggcggcgt gccttctctg attgccgttt
     3661 accaagacaa ttccggcaaa gccaaagaca tcgccctgtc ttatgcggct gccaacggcg
     3721 gcaccaaagg cggtgtgatt gaaaccactt tccgcgaaga aaccgaaacc gatctgttcg
     3781 gcgaacaagc cgtattgtgc ggcggcgtgg tcgagttgat caaggcgggt tttgaaaccc
     3841 tgaccgaagc cggttacgcg cctgaaatgg cttacttcga atgtctgcac gaaatgaaac
     3901 tgattgttga cctgattttc gaaggcggta ttgccaatat gaactactcc atttccaaca
     3961 atgcggagta cggcgaatac gttaccggcc ctgaagtggt caatgcttcc agcaaagaag
     4021 ccatgcgcaa tgccctgaaa cgcattcaaa ccggcgaata cgcaaaaatg tttatccaag
     4081 agggtaatgt caactatgcg tctatgactg cccgccgccg tctgaatgcc gaccaccaag
     4141 ttgaaaaagt cggcgcacaa ctgcgtgcca tgatgccttg gattactgcc aacaaattgg
     4201 ttgaccaaga caaaaactga ttgttttcaa acgggactac ctacacatcg tgtaggcagt
     4261 t
//
LOCUS       DAWGJV010000150         4188 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWGJV010000150 DAWGJV010000000
VERSION     DAWGJV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.150"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..138)
                     /locus_tag="WHQ94_001782"
     CDS             complement(<1..138)
                     /locus_tag="WHQ94_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ2129021.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YL"
     gene            complement(266..1507)
                     /locus_tag="WHQ94_001783"
     CDS             complement(266..1507)
                     /locus_tag="WHQ94_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ2129022.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     ETQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEAKVRKQVTALCNKYPVYGA"
     gene            complement(1574..1769)
                     /locus_tag="WHQ94_001784"
                     /pseudo
     CDS             complement(1574..1769)
                     /locus_tag="WHQ94_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1988..3763)
                     /gene="ggt"
                     /locus_tag="WHQ94_001785"
     CDS             complement(1988..3763)
                     /gene="ggt"
                     /locus_tag="WHQ94_001785"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ2129023.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3931..4167)
                     /locus_tag="WHQ94_001786"
     CDS             complement(3931..4167)
                     /locus_tag="WHQ94_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ2129024.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENLEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1046 a          981 c          995 g         1166 t
ORIGIN      
        1 gaggtaacgg gttctttgac ggttcaggta ttgttcgatc ggctgccaat caatcacttg
       61 atccaacttc aatagaggga aacggtcgat gtgtttggca atcatggctt gtgcggtttg
      121 ttgaaagaag gtgctcatga gaaatcccct aaatgtcttg gtgggaattt aggggatttt
      181 gggggatttt gcaaaggttt ccgcctgaaa cattatgaga tttcaggcgg cattggattg
      241 cttggcggaa tatttttaaa aaggcttacg cgccgtaaac ggggtattta ttgcacaaag
      301 cagttacttg tttgcggact ttggcttcgt cttcggggtt agacaatacg tcggcaacca
      361 agttcgccaa tacgcgcgcg tcggcttcgt taaaaccgcg tgtggtcatg gcggcggaac
      421 cgatgcggat gccggaggta acgaagggtt tttccggatc attcgggatg gcgtttttgt
      481 tgacagtgat gtgcgctttg cccaaagcag cttcggcggc tttgccggta attttcatcg
      541 gttgcaggtc aacgaggaaa acgtggcttt cggtgcggcc ggaaacgatg cgcaaaccgc
      601 gtttaaccaa ctcttccgcc atggcggctg cattgatttt cacttgtttt gcgtattgtt
      661 tgaactcggg ttgcaatgct tctttaaacg ccacggcttt ggcggcgata acgtgcatca
      721 gcgggccgcc ttgcaggctt gggaagatgg aagagttcaa cgctttttcg tgggtattgt
      781 cgcggcacaa aattacgccg ccgcgaggac cgcgcagggt tttgtgggtg gtggtggtaa
      841 cgaagtcgca gaatggcacc gggttgggat attcgccacc ggcaatcagt ccggcgtagt
      901 gcgccatgtc gacaaagagg tatgcgccga ctttatcggc gatttcgcgg aattttgccc
      961 agtcgatttg caacgcgtag gcagacgcac ccgccacaat cattttgggt ttgtgttcga
     1021 gcgcgaggcg ttcgacttcg gcataatcga gcacttcgtt ttcatccaaa ccataagtaa
     1081 cggcattgta gagtttgcct gagatattaa cgctcgcgcc gtgggtcagg tggccgccgt
     1141 gcgctagaga catacccaaa atggtgtcgc ctggttttaa aacggaagcg tacacggctt
     1201 ggttggcttg cgagccggag tgcggttgga cgttggcgta ttgcgcgccg aacagttttt
     1261 tgacgcggtc aatcgccaat tgttcgacaa tatcgacgta ttcgcagccg ccgtagtagc
     1321 gtttgccggg gtagccttcg gcgtatttgt tggtcagctg ggaaccttgc gtttccatta
     1381 cggcgcagct gacgtagttt tcagaggcaa tcagctcgac gtggtcttgc tggcgttggt
     1441 cttcttgggc aatggctgct gccaaatcgg ggtcatattg tgcgagggta acgctttttg
     1501 aaaacatgtt ctcggctcct ttgtgtaatc agggtatcat gagtgttttt tgtataaaaa
     1561 aatacttcaa aacctaaggc agatagtcca taatgcgtaa attttctttg gcattatcag
     1621 gtaatttatt taacatgctg gtttttagcg tctcattacc tttatgttag tacaaggcta
     1681 cccaaaggca atacatcaaa tggtaaattt tcggcagttt gtgcaaaatc aatcaaataa
     1741 ccagcaccaa ccacatcttt atcagccata acccgctgat acaccacttg ccaagacaac
     1801 tctcctgccc caccatgtct taaaatcaga tgaatggctt gtaatgctgt gtttggcgtc
     1861 tcacaaattg cgaagatctg ctcagaagct tgcttgatgg cttgtatttt tgtttgatta
     1921 ctcacaattt ccccctattt taatattaac ttaatgcggt caaattcaca aaatacaagc
     1981 ttcacctcta atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc
     2041 tgcttggctc gataataatg gcttgaacac ctgaatttag ctcacgcacg tcagtcttat
     2101 agcccaaatc atttaatgct tgttgccact ggagggcggt tgtacccgtt tctagttcat
     2161 agctaccaaa gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc
     2221 agtcgctatg tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg
     2281 agccgattgc catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc
     2341 gtggtctctt gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa
     2401 aactaaaatc tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa
     2461 acgcattttc aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata
     2521 tatgactggt agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc
     2581 ctgcagatac gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta
     2641 ggtagtcttt agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat
     2701 atacatcacg atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag
     2761 catcataccc cacctgattg ggtgaaaatt catttaaaat ccccaaaatc tgacccacag
     2821 caatcccacc tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac
     2881 aaacaggcgg gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg
     2941 ggttatcctt agcattttgg acaactgaaa cgatattttg ggcatattta ccagtatgca
     3001 gagcttttgc accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca
     3061 gcaagctgcc tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat
     3121 agcgtgccaa atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa
     3181 agccttgttt tgccaagcgg atcggtgtat caaataattt tccccaaggc aatacaccgt
     3241 atcgctgatg tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac
     3301 cgaccaccgc ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg
     3361 tcgcacgcat cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat
     3421 cccaatacaa cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta
     3481 gtgtcgtctg caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat
     3541 agccagcttg tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct
     3601 gcttttgttc ggtcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtgatga
     3661 cagcagaatg agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg
     3721 tcatagacat tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg
     3781 agtttgattg atgattcata caaggcatgc tgattattgt atttaatatg gctaaataat
     3841 tcaatccaaa ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt
     3901 ttttgatatt tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag
     3961 tggttttagc tcacctgatt ggtacatttg taggataata tcactaccac cgattaactc
     4021 accattaacc caaagctgtg gaaaggttgg ccgattggcg atgagtggta gagtactgcg
     4081 aatttctagg ttttctagga tattaacaaa agcaaagggt ctgccaattg ggtcagcacc
     4141 tctactgcac gcgctgaaaa tccacattgg ggaaactggg gcgtgcct
//
LOCUS       DAWGJV010000151         4149 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWGJV010000151 DAWGJV010000000
VERSION     DAWGJV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.151"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(163..1566)
                     /locus_tag="WHQ94_001787"
     CDS             complement(163..1566)
                     /locus_tag="WHQ94_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ2129025.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYARQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(1635..3062)
                     /locus_tag="WHQ94_001788"
     CDS             complement(1635..3062)
                     /locus_tag="WHQ94_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ2129026.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSVQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3298..3651
                     /locus_tag="WHQ94_001789"
     CDS             3298..3651
                     /locus_tag="WHQ94_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129027.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3940..>4149
                     /locus_tag="WHQ94_001790"
     CDS             3940..>4149
                     /locus_tag="WHQ94_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129028.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILR"
BASE COUNT          975 a         1104 c         1019 g         1051 t
ORIGIN      
        1 tatacatttg aattaattcg cacttaattt aaatgtgttt ttaactgtgc tttatttaaa
       61 ggcaatgaga atgtgaaagt atcggatcaa tcccaaagca gcctgcactt tcgaaacggg
      121 gtgcaggctg ctttgggaat ttcataaccg tttcagcctg ctttattccg caaataccgt
      181 ttccaaccct aacccgctct ctttcaccaa gcgcaaataa gccagcatga atttataccg
      241 tgcttgagcc agtttctgtt ctgcttgggc gacttcctgc cgcgcccgta ttacttccag
      301 ccggttgcgg atgccgtatt gttggccggt ttcggtcgat ttcagtttca aacggctgct
      361 ttccaaaacc cgttcttgcg ccatgatttg gtaacgcgcc gcaccgcttt cggtataagc
      421 ctggcgtacg gcgagtttga tgtgccgctc ggttgcggtc agctgtgctt cggcggcccc
      481 gtattgcgct tcggcttcat ggattttgcc cgacaattct ccgccggtat aaagcggcaa
      541 attcaactgt acgccgacgc tcatcccttt gccccgatag tggtagtcat tattctgcgc
      601 agatgaagtg tagaggttat tctgatagcc gacatgggca gaaacggtgg gatagcggct
      661 gttctgtgct gcccgaagcg cctgtccgct gctttgcagg gcaagctgct gcatccggta
      721 ttcatgattg ttggataagg caatgcgctg ccattcatcc agactgtaac gttccagctt
      781 gggcagatag cgtgccaaca ggttggcggt atctatggcc tcgatttgtt tgctatccag
      841 gtcggtgtag tcgttcaact ggttttcata ggtttgtttc tcagccaata cggcgatttc
      901 ttgggccagg gcattgtcgt aaccggcttt ggcttcgtga atatccagcg cggtggcagc
      961 acctttattg aataaagcct gcgcctgcct tacctgccgg gcataagcct ctttttccgc
     1021 cgcatgggcg gcaacggtgt ctcggctgag taaaacgttg aaataacttt cggcaacttt
     1081 caacagcaat tcttcgcgtg ccgcatcgaa acgctgttct gcagcctgcg tatcgaacct
     1141 gctttggcgg tattgtgcaa atttggcagc gtcaaataag gtttgtccca cctgcacgct
     1201 ccatccctgt gtttcgcggg tggaagaaat cgatggcggc tggcgctggt agctggcatt
     1261 ggcggataca tggggaagga atgcggcctt ggcttgttgt tgccgtgcgc gcactgcatc
     1321 acgctggtaa tgggacgctt gaaaatcagc cgaatgttgc tgcgccgccc gccatgcttc
     1381 aggcagcgta aaagccgaaa cggatgggga aagggatagt ggcaaggtaa aaagtgaaac
     1441 gggtaggata tatttggaaa aataggattt catagccgaa aatagttcat gttgcaaata
     1501 gggcgtcagt gtcaggcaaa cggaaatacc gtaatcttgc attatcatta gattgagcaa
     1561 tgtcatccgg gcaatggttt caggcagtct gcatgtccga accggcggat aacaaatgcc
     1621 cagtacggat ccgcctatcg ctccctaaag ctttcgtcca atttggtttg cagcgggctt
     1681 aacagataat ccagcacccg ccgtttaccc gttttaatct ccgccgtgac attcatgccc
     1741 gccgtcagat tcactgcttt gccgtcaata ttcaaggtat gtttgtccag cgacaccacc
     1801 gccgtataaa ccaagcccaa ctgttcgtgg cttaccgcat catggctgac acttttcacc
     1861 ttgcccgtca gataaccgta gcgcgtatag ggaaagctct caatcttcac caccgcatcc
     1921 tgtccctgtt ccacaaaacc gatgtctttg ttcaatacca aaacttccac gtccattttg
     1981 tcgtcatcgg gcgcaatcac catcattttt tgggcagcct gcaccacacc gcccaccgta
     2041 taggtagcca attcctgcac cgtgccgtcc gcaggcgact gtattgtcat cagctgctgc
     2101 cgctgctttg ccttatccgt ttggccgcgg tattggtcaa tctgttcgtt tgcctggcgc
     2161 agcgcatcca gcgtatcgcg tttcaggttc tgcgtattca gcacccgatt ctgctccgcc
     2221 tgtgcaatgg ccgcctgaat ctgcctcatc tgaccgcgcg tactttccaa atcgttccaa
     2281 ttgctgaccg atttgctctg ctgctccaaa aacgcatgtt ccgaaataaa attgtcggcc
     2341 cgcaaacggc ggtagtctgc tgttttctgc tgctcgatcg cccccaccga aaccagcttc
     2401 tgctcctgcg ccttggccga ctgcaattcc gcctgatggc cgcgcaaagc cgactgcaat
     2461 tgcgcatcct gcgccgccca tgcctgatac tggtgctgcg ccaacacctg caccgattgc
     2521 acatcggcat cggagagacc taaagaccgt gcttgcgcca tatcgatatg cggcacggta
     2581 cggctttcca atgccgccaa taccgcttca taacgcagtt tggacaattg ggcagcctgc
     2641 aaagcctgct ccgactgcac cacatcgctg tctgttccca cagcctccag ttccgccagc
     2701 gtttctccct gtttcacatg ctgcccgtcg cgcacatgta ccgccttaac caccgccgtt
     2761 tccagcggct ggatggtttt gctgcgcccg cccgacaccg ttttgcccga agccgccgcc
     2821 acaatatcga ttttgccgaa ccaggaccac aacaaagcca aaagcgcaaa cgccataata
     2881 aaacgcgccg cccatttcgg agcggcagag accggcgtat cggtcagttc caaatgcgcg
     2941 ggcaaaaacg cctgttcttc cgccgtgcgt ttgggcggtt tcaactggtc gcgcaccgcc
     3001 caaacattgc gccatacagt aatgtatcga gaaagaaagg atttcagggc ggagaaaaac
     3061 ataacgggta taaccttggc aatatagaaa caggaaacaa tataaatatg taaaggaatt
     3121 ttaacggaaa gcgcggcagc tgttaaggga aaggcgggaa tattgacaaa aaatacccaa
     3181 gtcgttacaa atattcatta ttttactgcg taacgcaacg ctgaagcgca ggctgctttt
     3241 gagatgcggc aaggttcggc aaaaagcagc ctgcacattt aaccacagga acaacccatg
     3301 tttaccacaa acgatttacg ccatttccta gaaggtttgg ccatcctatt ctcaatcggc
     3361 tattggggca ccatgctgct gttgctttgg tttctcgtcc gctttgccta taaaaagccc
     3421 aaacggaacc ccggcaaaat cctttcaggc agcctgaccg ccgccgctat cctgatgctg
     3481 tttgtttgga ttattcccaa acaattcggc ccgatcaaag aagaaataca ggcacaagaa
     3541 gagtgggaca gaaaatacaa agaagccgaa gccgtgttta acgaacaatg caaaacggcg
     3601 ggggaaagat ttaccagacg gcggacaatg tggaagggat tatgctgttg aaggtagtac
     3661 ctgagcgtac cgtttcggca gatgcaaaaa ccagagaccc gatgtgggac aatgcggctt
     3721 tacagaccag cgaaggcgta aattttattg ctcgtttcct aggatttttt agcgatgggg
     3781 aataccgcta tgtggatgtc ctgcaaccca accattccga tattattcgg tattcaggta
     3841 aagatttttc cgctaaatca aatacttaat catatacacc ccgcccgtta tgcggtaacg
     3901 ttcgaaaaca atgtcgattc caagctgcgc aggcactggg tggcaggtgc gaccatacgg
     3961 attatcgacc gccaaactga cgaagtgatt gccaagaaaa ccatctatgt ctttgaaaaa
     4021 ggcttggacg gcacgggtgg ggcgagaatg ccgtggaagt ttgctatctt gtgcaataaa
     4081 gaaagactta cttcttcaga gccgttatcg gattttgttc ttagcgtttt aaaaccttat
     4141 atattgcgt
//
LOCUS       DAWGJV010000152         4096 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWGJV010000152 DAWGJV010000000
VERSION     DAWGJV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.152"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(202..1098)
                     /gene="argB"
                     /locus_tag="WHQ94_001791"
     CDS             complement(202..1098)
                     /gene="argB"
                     /locus_tag="WHQ94_001791"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ2129029.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1268..2398
                     /locus_tag="WHQ94_001792"
     CDS             1268..2398
                     /locus_tag="WHQ94_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ2129030.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2469..3320
                     /locus_tag="WHQ94_001793"
     CDS             2469..3320
                     /locus_tag="WHQ94_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ2129031.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3403..4065)
                     /locus_tag="WHQ94_001794"
     CDS             complement(3403..4065)
                     /locus_tag="WHQ94_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ2129032.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          917 a         1195 c          987 g          997 t
ORIGIN      
        1 aaaatcaaag ccggagaatt tatcccaaac aactggattt caaaaaaacc agattcccgc
       61 cttcgcggga atgacggatt ttaggtttct gttttggttt ctgttttcgc gggaataaca
      121 acttagaaat tgcccgaaac cccaaaaaca gaaaccggac aaacgggttt tccgccaaag
      181 ccggcatttt ccgactttgc ttcaggcatc ttccccaccg cccaaaatca tcgaaccgat
      241 accggcatcg gtaaagattt ccagcaaaag cgcgttgggc aacctgccgt cgatgatatg
      301 cgtggctttc acaccgttga cggcggcttc gaccgcagaa gcgattttcg gcagcatacc
      361 gccatacagc gtgccgtcgg caatcagttc atcaatccgt ttcggcgtga gtttggtcag
      421 cagattgccc gttttgtcca tcacaccggc gatattcgtc atcatcaaga gtttttcagc
      481 gtttaattct tccgccaatt tgcctgccac caaatcggcg ttgatgttga acgcttcgcc
      541 tttttcacct acgccgacgg gggcgacgac gggaatgcag ccacgttcta tcagcccttt
      601 aaccaaacct gtatcaatgc tttccaccgt accgacctgt ccgatgtcca cgccattctg
      661 ttcgggcgta tcgatcaaaa gtttcttcgc cttaatgaaa tgatcgtcgc gtccgctcac
      721 gccgaccgcg tgtccgccat atgtgttaat catcgacacg atttctttat tgacatgccc
      781 acccaacacc atttcgacaa tatccatcgc ctctttgtcg gtaacgcgca ttccttggac
      841 aaactccccc tttttgccga ctttttcaag catcgcattg atctgcggcc cgccgccgtg
      901 aacgatgacg ggatgaatgc cgaccagctt cagcagtacg acatcgcggg caaacccttc
      961 tttcaaggca ggttcggtca tcgcgttgcc gccgtacttg atgacggcga ccgaaccgga
     1021 aaaccggcgg atgtaaggca gcgcttcggc aaggatacgc gctttgtcgg cggcggaaat
     1081 aatgttttca gactccataa cggctcccta ttgttgacaa tttcggctta tgttaacgcc
     1141 aaacgccgtc tgaaacaatc ttcagacggc atccggcaga ccttcgggca ggttgaagcc
     1201 ttgcgatacg ggcggtaaaa tcacagttta tccggttcga gaaaccggcg acagcaagga
     1261 ctttcccatg attaaaatca gcacccgctt cgatgccggc tcggtcgtcg tcaaagacct
     1321 gaccgatcct tccaacatcc gcctcgccct gcgtcccgac aacgcctccg attttgcaca
     1381 atggttctac ttccggctgc aaggcgcggc atatcaaaac tgcatcatgc attttgaaaa
     1441 cgcggcagaa gccgcctacc cgaaaggctg ggaaggctat caggcgtgtg cctcatacga
     1501 ccgccgcaac tggttccgcg taccgacttc ctacgaaaac ggtgtactga ccgtcaatca
     1561 tacgccgctg tccaacagcg tgtattacgc ctatttcgaa ccttactccg aagaacagca
     1621 tttaaacctc ctcggcgacg cgcaaggcag cggcctgtgc cgcatcgacg acttgggcag
     1681 caccgtgcaa gggcgcgaca tcaatctgct gaccatcggc aatcaggtcg aaagcgattt
     1741 gaaaatctgg attaccgccc gccagcatcc gggcgaaacc atggcggaat ggtttgtcga
     1801 ggggctgctc ggcaggctgc tcgatccgca agacccgaca gcgcgcaccc tgctcgaccg
     1861 cgccacgttc tatatcgtcc ccaatatgaa ccccgacggt tcggcactgg gcaaccttcg
     1921 taccaacgcc gccggtgcga acctcaaccg cgaatgggaa aatccgactt tggaaaaaag
     1981 ccccgaagtg ttcttcgtgc gcgaaaaaat gctggaaacc ggcgtggatt tgttcttgga
     2041 catccacggc gacgaagggc tgccctttat ctttgttgca ggaactgaag gcgtgccaaa
     2101 ctacaatccg cgcatttccg cgttggaaac acagttcaaa accgcccttt tgaacgccag
     2161 ccccgatttc caagacgaat acggttacga aaaagatgcg cccggtcagg caaacatgac
     2221 cttggcaaca aactgggtcg gcaaccgttt caactgcctt gcctatacgt tggaaatgcc
     2281 ctttaaagac aatgccaacc tgcccgacga cgatttcgga tggaacggtc aacggtcgtt
     2341 gcgcttgggc gaagccgcgt tgtccgccat cctcaacatc atcggcgatt tgcgctgaaa
     2401 cccttattgc agaaatgccg tctgaagcat catgcctttc agacggcatt tgaaaacgaa
     2461 agcccgttat gattacccat ccccaattcg accccgtcct tatcagtatc ggcccgcttg
     2521 ccgtccgctg gtatgcccta agctacatcc tcggatttat tctttttacc tttctcggca
     2581 gaaggcgcat cgcgcaaggc ttgtccgttt ttaccaaaga atcgctcgac gacttcctga
     2641 catggggcat tttgggcgta attttgggcg ggcgtttggg ttacgtcctg ttttacaagt
     2701 tttccgacta cctcgcccat ccgcttgata ttttcaaggt atgggaaggc ggaatgtcgt
     2761 tccacggcgg ctttttgggt gtagttattg ccatatggtt gttcggtcgc aaacacggca
     2821 tcggcttcct caaactgatg gacacggtcg caccgctcgt tccactgggt ctcgcttcgg
     2881 gacgtatcgg caacttcatc aacggcgaac tttggggacg cgttaccgac atcaacgcat
     2941 tttgggcaat gggcttcccg caggcgcgtt acgaagacct cgaagccgcc gcgcacaatc
     3001 cgctttgggc agaatggctg caacaatacg gtatgctgcc gcgtcatccc tcgcagcttt
     3061 atcagtttgc acttgaaggc atctgcctgt tcgccgtcgt ttggctgttc tctaaaaaac
     3121 agcggccgac cggacaagtc gcctcactct tcctcggcgg ctacggcata ttccgcttca
     3181 ttgccgaatt tgcacgccaa cccgacgact atctcgggct gctgaccttg gggctgtcga
     3241 tggggcaatg gttgagcgtc ccgatgattg ttttgggtat cgtcggcttt gtccggttcg
     3301 gcatgaaaaa acagcactga ccgcctgtga aaaataccgt ctgaaacatg gaaatccggt
     3361 ttcagacggc atttttacgt ttggttcatg gaaacaaacg cttcaattct taccgcaaca
     3421 cgccttgtat tttctgccgc tgccgcacgg acagggatcg ttcctgcctg ttttttcgcc
     3481 ttccctgcgg acggtttgcg gcttgttgat gaccgcctgc cagtagcggt agatatccgc
     3541 caatgcataa ggcaactcgg attccagttc cgccagctcg ccttctgtga attgcagacg
     3601 gatagtgccg ttgtcctctt cgtcgtaaat accgcccaat gccatgatgg gataaaacaa
     3661 ctcttcaaac gcttcatcat cgacggcttc aaaccaatcg gtcggcacaa tatccaaacc
     3721 gtaaagataa gcattgcacc atgtgtaaaa atcgctgccg ccgtcttcgt tttcatacag
     3781 ccacaaatcg ggcagttttt tatccgacat cgcggcggtt gtttccatcg ccattgccaa
     3841 aaccagccgt tcgatttcgg aacgttcggc ggcggtaaat tgcgattcgt cgcccaacac
     3901 ttcgggcagc cagtcgagcg gtgtcaattt gtccggcccg ctcaacagcg ccgtcataaa
     3961 accttgaacc tcgtcgcaac gcatcgtgtt gccttgttcg cttttggcat ccaacaattc
     4021 gctcaaccgc cgtttggatg cttcggtaaa tttttgggaa tccatcattt tccttttcaa
     4081 atgggttttg cgccct
//
LOCUS       DAWGJV010000153         4004 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWGJV010000153 DAWGJV010000000
VERSION     DAWGJV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.153"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(196..267)
                     /locus_tag="WHQ94_001795"
     tRNA            complement(196..267)
                     /locus_tag="WHQ94_001795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Arg,seq:cct)
     gene            complement(287..733)
                     /locus_tag="WHQ94_001796"
     CDS             complement(287..733)
                     /locus_tag="WHQ94_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ2129033.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WHQ94_001797"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WHQ94_001797"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ2129034.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDGKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1728..1916)
                     /locus_tag="WHQ94_001798"
     CDS             complement(1728..1916)
                     /locus_tag="WHQ94_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129035.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="WHQ94_001799"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="WHQ94_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ2129036.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="WHQ94_001800"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="WHQ94_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ2129037.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2776..3960
                     /gene="ubiM"
                     /locus_tag="WHQ94_001801"
     CDS             2776..3960
                     /gene="ubiM"
                     /locus_tag="WHQ94_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ2129038.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         1136 a         1111 c          885 g          872 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgga gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgcaaatcg tcaaacctcg ccaacaggaa tcgaacctgt attttacgct taggaggcat
      241 acgttctatc cgttgaacta tggcgagccg aaatgaaaag gagattttaa ccctttccga
      301 cgacaaaaga caagatgccg gccgcaatca gcggcccgac agggataccg cccataaatg
      361 ccaccccgat aaccgtcccg attaacagcc ctgtaattaa aacaggctgc tgtcccatca
      421 aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg gcggatatca
      481 ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa accaacggac
      541 tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccgtgcttc tcgaccaacg
      601 ggacaaactg taccaatgcc gtctgctgca tcagcagcaa tatggttgcc gagatggtaa
      661 tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac aggggaacaa
      721 aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaacgcgg caacggcaca atcgactgct
      841 ttgcgcgcct cgcctatcga atatgccaaa gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtaccgac atacataccg tagtctttca acgcctgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggatga cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagggtg gaagtgtgga taaattcgac catcgccgcc
     1501 agcgaataca gtttcccgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcca
     1801 tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggcatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
     2581 ctttttacat accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata
     2641 catcctcccc tgcatatttc ttcaacagcg ggttttgata taatcgccta tctgttacag
     2701 atagttcaaa cggcattcaa acccttacaa atgccgtctg aaatcatcgg catccgtcta
     2761 tgcaaaggat attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg
     2821 ggtttaagtt ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc
     2881 agcccactga ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat
     2941 ttttcccgcg aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc
     3001 taccctttgc gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc
     3061 caaccgactg aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac
     3121 aatatccgcc gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc
     3181 gatacggttg tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac
     3241 ggcaaaatcc tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt
     3301 cgacaactcg gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt
     3361 atgaaacata ccctttccaa ccaacatacc gcttacgaat gcttccatta cgggcgcacc
     3421 atcgcactgc tgccgctgga agaacacctg accaataccg tcattaccgt cgataccgat
     3481 aaaatcaaca gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag
     3541 ctcaagggca ggctcggcga tatggaactg gtcagcagta tccaccatta cccccttgtc
     3601 ggtatgattg ccaaacgttt ctacggtaaa cgcagcgcgc tgatcggcga tgccgccgtc
     3661 ggtatgcacc ccgtaacggc acacggcttc aacctcgggc tttccagcgc ggatattctg
     3721 gcaaaactga tactcgaagc cgaacagcgc ggtcaggaca tcggcgcggc ttccttgttg
     3781 gaaaaataca gcagcaaaca tatgttgcac gcccagccgc tttatcacgg aaccaatatg
     3841 atgctgaaac tgtttaccaa tgagaccgcg ccggcaaaac tgctgcgcgg tttggtattg
     3901 cgtgccggca acaacttccc gccgctgaaa aaactgatta ccaaacaact gaccggttga
     3961 aaactgccga aatgccgtct gaaacgtttc agacggcatt tttg
//
LOCUS       DAWGJV010000154         4003 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWGJV010000154 DAWGJV010000000
VERSION     DAWGJV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.154"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..632)
                     /locus_tag="WHQ94_001802"
     CDS             complement(<1..632)
                     /locus_tag="WHQ94_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ2129039.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKHN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFSAHPDAETPPPEN
                     SVKEALKRVGFGKVSFDDREIRFTEKGMGLSLNGIAQGYSGLTKTSTALPR"
     gene            complement(641..1435)
                     /locus_tag="WHQ94_001803"
     CDS             complement(641..1435)
                     /locus_tag="WHQ94_001803"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ2129040.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1858..3192
                     /gene="gdhA"
                     /locus_tag="WHQ94_001804"
     CDS             1858..3192
                     /gene="gdhA"
                     /locus_tag="WHQ94_001804"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ2129041.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3334..3708
                     /locus_tag="WHQ94_001805"
     CDS             3334..3708
                     /locus_tag="WHQ94_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129042.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3752..>4003
                     /locus_tag="WHQ94_001806"
     CDS             3752..>4003
                     /locus_tag="WHQ94_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ2129043.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1089 a         1045 c          936 g          933 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactatagc cttgcgcgat gccgttcaag
       61 gacaaaccca tacccttttc ggtaaaacgg atttcacggt cgtcaaacga gactttgccg
      121 aagccgaccc gcttcagggc ttccttcacg ctattctccg gcggcggcgt ttccgcatcg
      181 ggatgggcgg aaaaataatc ggcatacagt ttccacaaaa cctgaaccgt cggatcgaac
      241 gcgccttccg tcaacgcgtg aatatcgcgg cacaggctca acagttccaa aaaatccgcc
      301 gacggcgaag tcagataacc gtcacggttc aggcggctga tcaggctgtc ttcacggtaa
      361 aggctgaaca ttttttccaa acgcgccact tccgccaaaa ccttattgac caaatccgcc
      421 gcctgtctgt cgtccacgcc gaacagacgc agctccgcac cggaacccag cgcgacacct
      481 ttccagaaaa acacattttc attgtgtttt tcatctttat tgcgtttttc atcatcggcg
      541 gcaaaaggat tcggcaaaaa agaaaccgcc gcgcccgccg ccgcaacggc ggcaactgtc
      601 aaaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc ctaaaccgac actgccgcct
      661 tgatgtgcgg atgcggatcg tagccttcca actcaaaatc ttcaaatttg aaggcaaaca
      721 aatctttgac ttcaggattg attttcatca caggcaaggc gcgcggttcg cgttccaact
      781 gcaatgcggc ctgctcgaaa tggttgcggt acaggtgcgc gtcgccaaac gtatggacaa
      841 actcgcccgc ctccaatccg cacacttgcg ccatcatcat ggtcaacagc gcgtagctgg
      901 caatattaaa cggcacacca aggaaaatat ccgcgctgcg ctggtaaagc tggcaggaca
      961 gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca gggcggcaat gccatttcat
     1021 cgaccaaagc cggattccac gccgatacaa tcaggcggcg cgagtcgggg tttttcttaa
     1081 tttgttccac cacattggcg atttggtcga tatgcctgcc gtcgggcgcg ggccagttac
     1141 gccactggta gccgtaaacc gggcctaagt cgccgttttc gtccgcccac tcgtcccaaa
     1201 tggaaacatt gttgtccttt aggtatttga tattggtatc gcctttgaga aaccaaagca
     1261 gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt cagcagcgga aaacctttgc
     1321 ccaagtcaaa acgcatctga taaccgaata cggagcgcgt gcccgtaccg gtgcggtctg
     1381 atttgtccgt accgttgtcg aggacgtggc gcatcaagtc caaataggcc ttcatcatca
     1441 tctttcatca aattaaacgg cgcatattgt aacatttccg gccaacgccc gagtccggac
     1501 aaacgcacac gccgccccgc cggacgataa aaacgcagat acgggcaggc aggattgttg
     1561 gcaatttcag tccttttcca cagtaaaacc cagtgggaaa acaaaattac cttgattgga
     1621 atcaaaaaat ctagtttaat tacttagaat aaaatttcaa taatattttt atttacgaaa
     1681 ttaatttatg attattgatt aattatcggc aacaaacatc aaacatcgaa aacatggaaa
     1741 aaataatgtc aacaattttt gccaaatcgg gcttggcatc agaaaaaaat aggtttatat
     1801 tcccacctac aaatttgttt tcccattagt acactatcaa ccaaaaggag tatccgaatg
     1861 actgacctga acaccctgtt tgccaacctc aaacaacgca atcccaatca ggagccgttc
     1921 catcaggcgg ttgaagaagt cttcatgagt ctcgatccgt ttttggcaaa aaatccgaaa
     1981 tacacccagc aaagcctgct ggaacgcatc gtcgaacccg aacgcgtcgt catgttccgc
     2041 gtaacctggc aggacgataa agggcaagtc caagtcaacc ggggctaccg cgtgcaaatg
     2101 agttccgcca tcggtcctta caaaggcggc ttgcgcttcc atccgaccgt cgatttgggc
     2161 gtattgaaat tcctcgcttt tgaacaagtg ttcaaaaacg ccttgaccac cctgcctatg
     2221 ggcggcggca aaggcggttc cgacttcgac cccaaaggca aatccgatgc cgaagtaatg
     2281 cgcttctgcc aagcctttat gaccgaactc taccgccaca tcggcgcgga caccgatgtt
     2341 ccggccggcg acatcggtgt aggcgggcgc gaaatcggct acctgttcgg acaatacaaa
     2401 aaaatccgca acgagttttc ttccgtcctg accggcaaag gtttggaatg gggcggcagc
     2461 ctcatccgtc ccgaagcgac cggctacggc tgcgtctatt tcgcccaagc gatgctgcaa
     2521 acccgcaacg atagttttga aggcaaacgc gtcctgattt ccggttccgg caacgtggcg
     2581 caatacgccg ccgaaaaagc catccaactg ggcgcgaaag tgctgaccgt ttccgactcc
     2641 aacggcttcg tcctcttccc cgacagcggt atgaccgaag cgcaactcgc cgccttgatc
     2701 gaattgaaag aagtccgccg cgaacgcgtt gccacctacg ccaaagagca aggtctgcaa
     2761 tactttgaaa accaaaaacc gtggggcgtc gccgccgaaa tcgccctgcc ctgcgcgacc
     2821 cagaacgaat tggacgaaga agccgccaaa accctgttgg caaacggctg ctacgtcgtt
     2881 gccgaaggtg cgaatatgcc gtcgactttg ggcgcggtcg agcaatttat caaagccggc
     2941 atcctctacg ccccgggaaa agcctccaat gccggcggcg tagcaacttc aggtttggaa
     3001 atgagccaaa acgccatccg cctgtcttgg actcgtgaag aagtcgacca acgcctgttc
     3061 ggcatcatgc aaagcatcca cgaatcctgt ctgaaatacg gcaaagtcgg cgacacagta
     3121 aactacgtca atggtgcgaa cattgccggt ttcgtcaaag ttgccgatgc catgctggcg
     3181 caaggcttct aagcaaacgc cgccgctccg caaacaaaat gccgtccgaa ccgcaaatgc
     3241 tgttcagacg gcatttcatt atccgcccgt tcgaatcggg tgatactatt tgtatccaga
     3301 attgttggat agtttgggag tggagaggaa gaaatgatta tggaccaata ctatatggag
     3361 ttaaagaata agttatctaa cagaccaatc ctactggata atactaatga ttttcttttt
     3421 gttttagtaa atacagtgaa agctatgata gaaaatactg ataaaagtca gctttcagag
     3481 ttagataaaa ttttagacgg tgtcacaagt caagaattga aacttgccta tgatttttgt
     3541 caagggaaat tcggtcaagc aggtttttct tatcgtaggc acccgaatta tttttatttg
     3601 tccagtttaa ttgcgacttt tccagaattt gagttgtcta aagcagaccg agattacctc
     3661 aaaggaatca taaattttga taactacctt ttatatgagt tagattagtc tttaatcacc
     3721 tcaaaaccct atttattcaa aaggagaaaa aatgattgag ctgaaattag tagatgagag
     3781 cagttttcag gcagtgctgg atttgaaaat atctgaagct gatgaacgag cacgttttgt
     3841 ggctccaaat gtacgctctt tagctgacgc atggctctac cgggaaaatg aagatgtgtt
     3901 tccgagggca atctattggg ataagcaagt ggttggcttt ctcctgctgg aaatagacaa
     3961 ggatgaagca gaatacttta tctggcggat aatgattggt cag
//
LOCUS       DAWGJV010000155         3990 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWGJV010000155 DAWGJV010000000
VERSION     DAWGJV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.155"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(160..330)
                     /locus_tag="WHQ94_001807"
     CDS             complement(160..330)
                     /locus_tag="WHQ94_001807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129044.1"
                     /translation="MTVATTYPGMTAKGLQTASGGISCLPLKQFAAQLFKRVRYVSTH
                     RTTHKAPPYVSS"
     gene            complement(1071..1538)
                     /gene="rnhA"
                     /locus_tag="WHQ94_001808"
     CDS             complement(1071..1538)
                     /gene="rnhA"
                     /locus_tag="WHQ94_001808"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ2129045.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHVGHAENERADDLANRGAAQFSRLPLRQENAV"
     gene            1652..2521
                     /gene="tehB"
                     /locus_tag="WHQ94_001809"
     CDS             1652..2521
                     /gene="tehB"
                     /locus_tag="WHQ94_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ2129046.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSMYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGKFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2571..3377)
                     /gene="thiD"
                     /locus_tag="WHQ94_001810"
     CDS             complement(2571..3377)
                     /gene="thiD"
                     /locus_tag="WHQ94_001810"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ2129047.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIESRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSLRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          877 a          980 c         1121 g         1012 t
ORIGIN      
        1 aagttaatcc actatagttt aatttgtctg tccgcattgc cgctaattgt accacttcat
       61 cgtgtttcct tttcggttga aaccccgcca ctcggacatc tgtccttcgg ggcggcagga
      121 tcagacttta tttgggaggg gggtgtaacc ccttccaaat caggacgaca catagggcgg
      181 tgctttatgt gtcgtcctgt gtgttgaaac atatcggacg cgtttgaaca gttgtgcggc
      241 aaactgtttt agcggcaggc aagagatgcc gcctgatgcc gtctgaagcc ccttagccgt
      301 cattcccggg taggtagttg ctacggtcac taaaatcccg tcattccctc aaaaacagaa
      361 aatcgaaatc agaaacctaa aattcgtcat tcccgcgcag gcgggaatct aggtctgtcg
      421 gcacggaaac ttatcgggta aaacggtttc tttagatttt atgttctgga ttcccgcctg
      481 cgcgggaatg acggtgggag aacgatgccg tctgaagctc gacaaggccc ccaatgccgt
      541 ctgaaacctc gtcattcctc tgctgtccgc aacttttcgt cattcccgtg aaaacgggaa
      601 tctagaactt caaactttca gataatcttt gaatattgcc gttgcccgac gttctggatt
      661 cccgcctacg cgggaatgac ggcagagcgg tttctgttgc tcccgataaa tgccgcaatc
      721 tcaaatcccg ttattctctc aaaaacagaa aaccaaaatc agaaacctaa aatcctgtca
      781 ttcccgcgca ggcgggaatc taggtctgtc ggcacagaaa cttatcgggt aaaacggttt
      841 ctttagattt tgtgttctag attcccgctt tcgcgggaat gacggggcgg gcggatgctg
      901 tctgaagctc cgtcattcct gcaacttttc gtcattcccg tgaaaacggg aatctagaac
      961 ttttaaattt tcagacagct tttgaatatt gccgttgccc gacggtctgg attcccgcct
     1021 gcgcgggaat gacggtggag gacgatgccg tctgaagccc gttggcggtt tcagacggca
     1081 ttttcttgtc ggagcggcag tcgggaaaac tgcgccgcgc cacggtttgc caaatcgtcg
     1141 gcgcgttcgt tttcggcgtg tccgacgtgt cctttaaccc aagtccagct gacttggtgc
     1201 cgcccaacca gagcgtcgag ttctttccac aagtcgtcgt ttttgacggg ctgtttggcg
     1261 gcggttttcc agccgttgcg cttccaaccg tgtatccagt tttccatgcc gtttttgacg
     1321 tattgcgagt cggtgcagat gatgacggtg cagcgacgtt tgagggattt cagtccttcg
     1381 atgacggcgg tcagctccat gcggttgttg gtggtttgcg cttcgccgcc gaaaagttct
     1441 ttttcgtggc tgccgtagcg cattaatacg ccccagccgc ccgcgccggg attgcctttg
     1501 cacgcgccgt cggtgtaaag gtaaacggtt ttgtccatta ttgtgccttt gtgtggtgtc
     1561 cggcgtgatt ataagcggtt tgcgcgccgt gccgtctgaa agattgcggc ggattgaaat
     1621 aggcatataa tgtgcaactt tatttttgaa tatgaaggag cggatagtgg gtcaaagtgg
     1681 cgagttgttt tgtttcgggc aaatgcccgt atggaaggcg gaaaacctgc cggaagtttt
     1741 gttgtcgggc tattcgtccg aggaagggga atgggtctgc ctgaatgtgt tgcagggcga
     1801 tgtcgaagtc cgtgcaccgg acgggtcggc agaggtttgg tcggcagacg gcggcgattg
     1861 tgtgtttgcg ccgcagcagg tgttttcggt caaaccgaaa acggacgatg ccgaaatccg
     1921 cttgtcgatg tattgcgcgg cggcagacta ttttcacaaa aaatacggca tgagtgccac
     1981 gcattctgcg gtcgcggcgg ctcaggatac cgtaccggcg ggcagggcgt tggatatggg
     2041 ctgcggacag gggcgcaacg cgctgtttct cggtttgaag ggatttgaag ttactgcagt
     2101 cgatcacaat cccgccgcct tggcaaacgt ggcggagctg gcagaggcgg agggtttgaa
     2161 cgtccgcacg ctggaatatg atttgaatgc cgctgcttta caaggcaaat ttgattatat
     2221 tgtggcaaca gtggtgctga tgttcctgat gccgcagcgc gtgcccgacg tgattgccga
     2281 tatgcaggcg catacggcgg ctggagggta caatttgatt gtatcggcaa tggatacggc
     2341 ggatttcccc tgtccgatgc cgttcccttt taaatttaaa gagggggaac tgaaggatta
     2401 ttatcgggat tgggagctgg tcgaatacaa agaagaattg ggttctatgc acgccaaaga
     2461 cgaaaacggc aatccgatac ggtttaaatt tgtaaccatg ctggcgaaaa agcctgaata
     2521 atccttaaaa atgccgtctg aacattttgt ttcagacggc atttttacgt ctagtcccga
     2581 tacgcccaat gattgaccgg accgtgtccc gcgccgattt ccaaagggtt tgagattgcc
     2641 gccgtaatgt aggacttggc agtccgtacg gctttgcaaa cgtccgagcc ttttgccaac
     2701 tcggcggtga tgcaggcgga aaacgtgcag cccgtgccgt gcgtgtgggc ggttggaaag
     2761 cggaggctgt ccaattccag cgtttcattt tgcgtaaaca gccaatccgt gcagcgtccg
     2821 cttgtgctac cgttcaaatg tccgcctttg ataacgacat ttttgacacc gtaatcaagc
     2881 aggatttttg ccgcacgttc cgcatcttta cggctttcga tatgcacgcc ggtcagggct
     2941 tccgcttcgg gcaggttggg ggtcaacaca tccgtatcgg gaagcagcag gcgcgtcagt
     3001 gccgcaacgg cggaatcctg caacagcggc gcaccgcctt tggcaatcat cacagggtcg
     3061 agtacgcgcc tgccgaagtc acagtgtttc agcttgtcgg caacgcattc gatgatttcc
     3121 gccgtgccga gcattcctat tttgtaggcg cggatgtcaa agtcttccct gattgcttgg
     3181 atttgtgcgg tgatggtttc ggtcgggacg agatgaaccg ccgacacgcc caaggtattt
     3241 tgcgcggtaa cggcggtgat gacgcacgtt ccgaacacgc cgcgcatctg aaacgttttc
     3301 aaatccgcct gaatgcccgc accgccgccc gaatccgaac cggcgatggt aagcgtttgc
     3361 acaaaagagc ttttcatttg ttcctcctta atattgtgtc cgataaaaag ggctaatgcc
     3421 tgtaaaaaag cacggcgatg ccgtctgaaa accgcagcgc ggggtttgtg gtgcattata
     3481 tgccgtattg caggacaagg cacagggcag ggcggcgggg tgcatcgtag caactgtatt
     3541 tttgcccgac ggggtgaaaa atacagttgc tacacccact gataatgaaa aaagagagca
     3601 atgccgtctg aaccgcgtgt tgcggcgagc ataaaccggc acgctttatt cgtcattccc
     3661 acaaaaacag aaacctaaaa ttccgtcatt cctacgaaag cgggaatcca gtgtgttcgg
     3721 tttcagttgt ttttgggttt cgggtgattt ccaaatcatc attcccgcgc aggcgggaat
     3781 ctagcccttg gggcagcggc aatatttata aagccgtctg aaaattcaga agttctagat
     3841 tcccgttttc acgggaatga cgaagggttg tgggaatgac gaagtttcag acggcatcgc
     3901 cccgccccgt cattcccgca taagcgggaa tccaaaccat tgggcagagc agccttaaag
     3961 gttataaaag actcgtcatt cccacgaaag
//
LOCUS       DAWGJV010000156         3893 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWGJV010000156 DAWGJV010000000
VERSION     DAWGJV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.156"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="WHQ94_001811"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="WHQ94_001811"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ2129048.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPYTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1380..1838)
                     /locus_tag="WHQ94_001812"
     CDS             complement(1380..1838)
                     /locus_tag="WHQ94_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ2129049.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQATASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            2108..2929
                     /locus_tag="WHQ94_001813"
     CDS             2108..2929
                     /locus_tag="WHQ94_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2129050.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2980..3426)
                     /gene="yciA"
                     /locus_tag="WHQ94_001814"
     CDS             complement(2980..3426)
                     /gene="yciA"
                     /locus_tag="WHQ94_001814"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ2129051.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          905 a          842 c         1141 g         1005 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgcctgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaca aactcttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgctatacc atattgcaga tgaaggtggc ggaaaggatt
      301 atggcgcagc atccggggga gcggttttat gtggtgctga tgtctgagaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ttttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggtg
      481 aaggcaatgc tgctgccgaa ggtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 atcaatttaa ttcaaagcag cagctatttg ggtgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgtaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga aggcggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaggaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaacat acaatatgtc gcaccgcacc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgaaatt
     1021 aagaaaaacc cgtatacgag gtatgaaatt tatacctttt tcagcggtgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaag attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tccgattgct ttgcgcgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagg cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaaact gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcatccag tttgccggtt tgggcagcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagg
     1561 gcgcggccgt tgccttgcgg atcggactca aaaagtgtga acggtttctt gctgctttcg
     1621 gcaaacgacg ctgtcgcctg tttgaatttt tcgacatcgt aaggctcttc gcctttgacc
     1681 attttgccca tacgcgagaa gtcgggcatc atagatttga acgcggcggt gcggttttcg
     1741 gaaatttcac ctttgggctg tgcgggcgcg ccgccgtttc cgcaggcgga aaggaatagg
     1801 gcgagggcgg ttgcggcaag gcgggtttga atattcatcg tgtttccttt tcggttgaaa
     1861 ccccgcccac ttggacatcc gtccttcggg gcggtagaat cagattttat ttgggaaggg
     1921 tgcaatccct tccgaatcag gacggcacat agggcgacgc tttatgtgcc gtcctgtgtg
     1981 ttgaaacata ttgaatgtgt cctgtcgtgg tggtatggtt gttgtcattt tcagccggcg
     2041 cgatgccgtc tgaagagcgt tatgatacct gaaacaggtt tgggaaacga gaaagaaagg
     2101 aaaaacaatg gctgttttaa ttaccggtgc ttcggcagga ttcggcgaag cgatgtgccg
     2161 cgcgtttgtc ggggcgggat accgcgttat cggtgcggcg cgccgtgcgg acaggcttca
     2221 ggccttggcg gatgaattgg gtgctttgtt ttaccctttg gaaatggacg tgtcgcgacg
     2281 cgagtcggtg gaaaacgcct taaacggcat ccccgatgaa ttttccgaca tcgactgcct
     2341 catcaacaat gccgggctgg ctttgggtct ggacacggcg gacaaggcgg attttgaaga
     2401 ttgggaaacg atgattcaaa ccaatgtttt gggtttgacg ttcctgacgc gcaaaatttt
     2461 gccgcaaatg gtggaacgcg gcggcggcta tgtgatgaat ttgggttcga ttgcaggcaa
     2521 ttatgcttat gccggcagca acgtttacgg ggcgaccaag gcgtttgtgc ggcagttcag
     2581 cctgaatttg cgcgcggagt tggcggataa gaacatccgc gttaccaata tcgagccggg
     2641 tttgtgcggc aatacggagt tttccaatgt gcgcttcaaa ggcgatgacg agagggcggc
     2701 gggcgtgtat gagggtgtgg aatttatccg ccccgaagat attgcggaaa ccgcattgtg
     2761 gctgtaccag cggccggcgc atatgaatgt gaacacgatt gaaattatgc ccgtggcgca
     2821 gacttttgca ggaatgaaag tgataaaaaa agccgtgccc gaagtgcggg aagactttga
     2881 aaaacagagt atgtcgctgt tttcccgcat caggtcctgg ttcaaatgat gcaaaatgcc
     2941 gtctgaagac agtttcagac ggcattttta cgggtatttt tacggagtag gcaataagcc
     3001 cgccaatttg gggttgcctt ctttcggaat cgggcgcgga ttgccttccg catcgatggc
     3061 aacataagtg aacacggctt cggttacgag gtagcggtct tcggtaacgc aatcgttcat
     3121 caaagttttc acccagacgt cgactttaag ctggagggaa gtgttgccca cgcggacgca
     3181 atgcccgtag cagcagacga cgttgccgac cttgaccggg cggatgaagt tcatttcctg
     3241 aacggcgacg gtaacgatgc gtccccgcgc gatttccgcc gccaatatgc cgccgcccaa
     3301 atccatctgc gacataatcc agccgccgaa aatgtcttgg ttgggattgg tatcgcgcgg
     3361 catagcgacg gtacgcagga gcagttcgcc ttgagggcgt tggcggttgc cttcttcgtg
     3421 ctgcataaag tttccttgtt ttattgaaat ataaatcgaa cctgcacccc tgcccgaaac
     3481 gggaagtcgg gaagtgtgta gtttaaccca tttgagacat acaagggcgg gcgcggaaca
     3541 gattcgcaag gcgtattgta gggtggggcc gtagcaactg aaccgtcatt cccacggaag
     3601 tgggaatcta gaacgagggg tttgagcaac tgtttttatc cgataagttt ctgtgcggac
     3661 aggtctggat tcccgcctgc gcgggaatga cgggtttcga gattgcggtg ttgtcggaac
     3721 gcaactgaac cgtcattccc acggaagtgg gaatctagaa cgcggggttt gggcaactgt
     3781 ttttatccga taagtttctg tgcggacagg tctggattcc cgcctgcgcg ggaatgacga
     3841 atttcgagat tacggtgttg tcgggaatga cgggtttcaa gattgcgctg tta
//
LOCUS       DAWGJV010000157         3876 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWGJV010000157 DAWGJV010000000
VERSION     DAWGJV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.157"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(105..299)
                     /locus_tag="WHQ94_001815"
     CDS             complement(105..299)
                     /locus_tag="WHQ94_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ2129052.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(289..2370)
                     /locus_tag="WHQ94_001816"
     CDS             complement(289..2370)
                     /locus_tag="WHQ94_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ2129053.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2846..3226
                     /locus_tag="WHQ94_001817"
     CDS             2846..3226
                     /locus_tag="WHQ94_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ2129054.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGRRGYMPPVQNQAGPNDF
                     RAFSCENGLSVRVRRLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3359..3852)
                     /locus_tag="WHQ94_001818"
                     /pseudo
     CDS             complement(3359..3852)
                     /locus_tag="WHQ94_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          905 a          969 c         1098 g          904 t
ORIGIN      
        1 gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat attataaaaa
       61 ttccgtctga agtggcgttc agacggaatt tgtttcaagc aggcttaaca gcagcgtccg
      121 ccgtttgcgc cgcaacggcg cttgcggcag tagtcttgaa attgcagctt ggtcatcacg
      181 ggggcgttgg gattatgttt gcgctgctgt gcaacgtagt tttcataatc aggcacgcct
      241 gccatcaagt ttgccgtcag cttgatggtt ttccaccaag acgcgagctt atgcttcact
      301 ttgtgcctcc ggctgcttgc cgtcgcggta caccgccggg atttctttgg cggtcggcca
      361 gccgactttg cgtgctttga gggcggtacg caaaccgtac gcggcgacaa tcacgacaac
      421 cgacaagaag aggatggtca gaccggcatt aatcttgtcg ttgaagatga tttgcgccat
      481 ttcgccgatg tctttggcag gcgcaaggac ttcgttttta gccaatgcgt cgctgtattt
      541 gccggcgtgg gcaaggaagc tgatgcgcgg gtcgctgtgg aacagttttt gcaggccggc
      601 gtagcaggtt acgaacagta cgccgacggc gggaacgagt accacccaaa cataacggtc
      661 gcgtttcatc ttaatcagca ccacggcgca cataatcaag gccacgcctg ccagcatttg
      721 gttggcgatg ccgaacaaag gccagagcga gttgatgccg cccagcgggt cggttacgcc
      781 ggtgtagagg aagtagcccc acaatgccac ggcgaagaag gtcgcaatca ggttggcggg
      841 gatggagtcg gtgttgccga aaggtttgta gaagatgctg cccaagtctt gaatcataaa
      901 acgtgcgacg cgcgtaccgg catcgacggc ggtcaggatg aacaaggctt caaacaatag
      961 ggcgaagtga taccagaacg ccatcatcgc ctcgcccgga atcaggcggc tcataatgtg
     1021 cgccataccg actgcgaggg ttggcgcacc gcctgcacgg gaaaggatgg tgttttcgcc
     1081 cacttcctta gcggtatgca acagggttgc cgcatcgaca gggaattgca gcttggtggt
     1141 aatcacttcg gcggcggtgt tggcatccgt accgatcagg gcggcagggc tgttcatggc
     1201 gaagtacacg ccgggatcaa gcgatgcggc ggcggcaagc gccataatgg ctacgaaact
     1261 ttccatcaac ataccgccgt aaccaatcat acggacgtgg gtttcgtttt ccagcatttt
     1321 cggcgtggtg ccggaggaaa tcagcgcgtg gaagcccgaa accgcaccgc aggcgatggt
     1381 gatgaagagg aacgggaaca atgcgcctga gaataccgga cccgaaccgt cgataaagtg
     1441 ggttacggca ggcatttgca aagtgggatt gacgatgacg atgcccaaag ccaaggccgc
     1501 aatcgtaccg attttcagga aggtggagag atagtcgcgc ggagtgagca gcaaccatac
     1561 gggcaatacg gaggcgacaa agccgtaaat cataatcgcc caagtgagct ggatgccgtc
     1621 aaggtcgaac caatgtccga tggaactttt cgccacatct tcgccgtaaa ttaccgccag
     1681 catcagcaaa ataaagccga cgatggaaat ctcgccgatt ttgcccggac ggatatagcg
     1741 cgtgtaaata cccataaaca gggcaatcgg catagtggcg gcgatggtaa acgtacccca
     1801 agggctgtga acaagtgctt ttacgacaat caacgccaac accgccataa tgatgaccat
     1861 aatcatcaaa ataccgatgg aggcaatcac gccggggaca gtgccgagtt cctgtttcac
     1921 aatatcgccc aaagacttac cgtcacggcg catagaaaca aacagtacca tcatatcctg
     1981 taccgcgccg gcaaaaacca cgccgaagat aatccacaaa gtaccgggca ggtaacccat
     2041 ttgcgcagcc agtacagggc cgaccaaagg gcccgcgccg gcaatcgcgg caaagtggtg
     2101 tccgaacaat acgcccttgt gcgtcggaac gtagtccaag ccgtcgttgt ggcgttctgc
     2161 cggagtcagg cggttaggat cgagccgcat tacgcggttg gcgatgtaga ggctgtaaaa
     2221 acggtaggcg atgcagtaaa cggatatggc ggcggtaacc atccataccg cgctgacctg
     2281 ttcgcctcgg ctgagagcca gagtggtaaa ggatgctaag ccgaccagta ccactatgcc
     2341 ccaaatgagg aaggttttga gtgatttcat cgaataaatc cttatctcac actgtcggaa
     2401 tatgcctgaa cggcgggaaa cggatgaccg cctgccggca cgggcacaaa ccggagtctg
     2461 aatgattgac gaagtatgaa acagtgcgct tgtcgggatt tgtgccttat gcccgcatca
     2521 aacagtgcag ggatgcgggc gcacaaaacg ttaaacgccg aataggattt taacgcaaat
     2581 tagcacactg atagcggttt ttacttggaa tttggaaaaa atttacattc cttcgggcgg
     2641 acaggcgggt ttggatggca tcgtcaggca aaaggcggcg ccggaagacg ggtaaagaag
     2701 gggcgcgcaa tccggattat tgattcatcg cgggtaaatt ccggttatcg ggcttgtgtg
     2761 tttgcgcgtc cgtttatagt atggcgttgc cgcagcttgg aatcagggcg gttgtttcat
     2821 atcttatttt tattggggag cttttatgaa tatcaggttt ttcgcgctga ccgtaccggt
     2881 tttgtctttg gcggcctgtg ccgtgccgga ggcgtatgat gacggcagac gcgggtatat
     2941 gccgcctgtt caaaaccaag ccggcccgaa cgattttcgg gcgttttcat gcgagaacgg
     3001 tttgtctgtg cgcgtccgcc gtttggacgg tggcaaaatc gcgttgcggc tggacggcag
     3061 gcgtgccgtc ctctcttccg acgttgccgc atccggcgaa cgctataccg ccgaacacgg
     3121 tttgtttgga aacggaaccg agtggcacca gaaaggcggc gaagcctttt tcggctttac
     3181 cgatgcctac ggcaattcgg tcgaaacttc ctgccgcgcc cgttaaacgg ggcagcctgt
     3241 ttgaaccgcc gtccggattt ttcgggcggt tttttgtgtc ggatttgttt tggctgtcgg
     3301 ctggtttcgg cggttttcgg gcgtggcgga ttatttttcg tttcgttccg ggtttatcct
     3361 atcggcagcg gctcaatgcc aactttaaac ctgctccgat ttcttcaggg ctgttatcca
     3421 atgataaaat gacatcgtct gcatcaatgg catcccacgc ttccagcttg acatggcggc
     3481 tcgggctgat tttcaggcag ccgttgtgca gccaaatatc cccgctcatc atgtttttaa
     3541 atagggcgcg tctggtttta tagcccaagt tcccgcatag tttggcaacc caatcctcat
     3601 agcgttgccg aaatttttcg gtatcaaaaa aatcttggtc ttccgaactg tcataaacga
     3661 aagtcctgct gtttgccaac gcttgcaaga ccgtcgtgcc taaagtttca ttgtcggtat
     3721 ccaatggcag gatatggggg gatataggtg gtctattgcc gttaacccca aacctgaaca
     3781 tgtttgaaca atcagagctt cttcattagc ataaaaaact gcccaataat tttgatcctg
     3841 tttaaaaatt attatttgat ctccgtaatt ttgatt
//
LOCUS       DAWGJV010000158         3784 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWGJV010000158 DAWGJV010000000
VERSION     DAWGJV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.158"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            346..3540
                     /gene="nalP"
                     /locus_tag="WHQ94_001819"
     CDS             346..3540
                     /gene="nalP"
                     /locus_tag="WHQ94_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ2129055.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGTSAPDFNAGGTG
                     IGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPNLHTGDF
                     TNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNENYK
                     NYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIGGRS
                     VDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTTSRA
                     GTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFIFSA
                     SNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAPYEA
                     SVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAVGVD
                     SKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGSQLQ
                     LHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYLADT
                     DRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYLNRT
                     GQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNAART
                     ASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGIRPY
                     GATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHNGTG
                     LRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSENSA
                     NAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLMQLG
                     ALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGLKL
                     SQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGAD
                     VEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1026 a         1024 c         1004 g          730 t
ORIGIN      
        1 gaaccgattc acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact
       61 ggtttttgtt catccactat aactagggaa attcaaatta agttagaatt atccctataa
      121 gaaaaagccg tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt
      181 caaatttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc
      241 cctataataa tataattcaa aatataaaaa tgggttacat ctaaacatta cggaattttt
      301 attccctcgc ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca
      361 accttcccta caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt
      421 tctgcctgct taggcggcgg cacttctgcg cccgacttca atgcaggcgg caccggtatc
      481 ggcagcaaca gcagggcaac aacagcgaaa tcagcagcag tatcttacgc cggtatcaag
      541 aacgaaatgt gcaaagacag aagcatgctc tgtgccggtc gggatgacgt tgcggttaca
      601 gacagggatg ccaaaatcaa tgcccccccg aatctgcata ccggagactt tacaaaccca
      661 aatgacgcat acaagaattt gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      721 cgcggggtag aggtaggtat cgtcgacaca ggcgaatccg tcggcagcat atcctttccc
      781 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacta tacggcgtat
      841 atgcggaagg aagcgcctga agacggagac ggtaaagaca ttgaagcttc tttcgacgat
      901 gaggccgtta tagagactga agcaaagccg acggatatcc gccacgtaaa agaaatcgga
      961 cacatcgatg tggtctccca tattattggc gggcgttccg tggacggcag acctgcaggc
     1021 ggtattgcgc ccgatgcgac gctacacata atgaatacgc atgatggaac caagaacgaa
     1081 ataatgtctg cagccatccg caatgcatgg gtcaagctgg gcgaacgtgg cgtgcgcatc
     1141 gtcaataaca gttttggaac aacatcgagg gcaggcactg ccgaccattt ccaaatagcc
     1201 aattcggagg agcagtaccg ccaagcgttg ctcgcctatt ccggcggtga taaaacagac
     1261 gagggtatcc gcctgatgca acagagcgat tacggcaact tgtcctacca catccgtaat
     1321 aaaaacatgc ttttcatttt ttcggcaagc aatgacgcac aagctcagcc caacacactg
     1381 accctattgc cattttatga aaaagatgct caaaaaggca ttatcacagt cgcaggcgta
     1441 gaccgcagtg gagaaaagtt caatggctcc aaccattgcg gaattactgc catgtggtgc
     1501 ctatcggcac cctatgaagc aagcgtccgt ttcacccgta caaacccgat tcaaattgcc
     1561 ggaacatcct tttccgcacc catcgtaacc ggcacggcgg ctctgctgct gcagaaatac
     1621 ccgtggatga gcaacgacaa cctgcgtacc acgctgctga caacggctca ggacatcggt
     1681 gcagtcggcg tggacagcaa gttcggctgg ggactgctgg atgcgggtaa ggccatgaac
     1741 ggacccgcgt cctttccgtt cggcgacttt accgccgata cgaaaggtac atccgatatt
     1801 gcctactcct tccgtaacga catttcaggc acgggcggcc tgatcaaaaa aggcggcagc
     1861 caactgcaac tgcacggcaa caacacctat acgggcaaaa ccattatcga aggcggttcg
     1921 ctggtgttgt acggcaacaa caaatcggat atgcgcgtcg aaaccaaagg tgcgctgatt
     1981 tataacgggg cggcatccgg cggtagcctg aacagcgacg gcattgtcta tctggcagat
     2041 accgaccgat ccggcgcaaa cgaaaccgtg cacatcaaag gcgatctgca gctgggcggc
     2101 gaaggtacgc tgtacacacg tttgggcaaa ctgctgaaag tggacggtac ggcgatgacc
     2161 ggcggcaagc tgtacatgtc ggcacgcggc aaaggggcag gctatctcaa ccgtaccgga
     2221 caacgtgttc ccttcctgag tgccgccaaa atcgggcggg attattcttt cttcacaaac
     2281 atcgaaaccg acggtggtct gctggcttcc ctcgacagcg tcgaaaaaac agcgggcagt
     2341 gaaggcgaca cgctgtccta ttatgtccgt cgcggcaatg cggcacggac tgcttcggca
     2401 gcggcacatt ccgcgcccgc cggtctgaaa cacgccgtag aacagggcgg cagcaatctg
     2461 gaaaacctga tggtcgaact ggatgcctcc gaatcatccg caacacccga gacggttgaa
     2521 actgcggccg ccgaccgcac agatatgccg ggcatccgcc cctacggcgc aactttccgc
     2581 gcagcggcag ccgtacagca tgcgaatgcc gccgacggtg tacgcatctt caacagtctc
     2641 gccgctaccg tctatgccga cagtaccgcc gcccatgccg atatgcaggg acgccggctg
     2701 aaagccgtat cggacgggtt ggaccacaac ggcacgggtc tgcgcgtcat cgcgcaaacc
     2761 caacaggacg gtggaacgtg ggaacagggc ggtgttgaag gcaaaatgcg cggcagtacc
     2821 caaaccgtcg gcattgccgc gaaaaccggc gaaaatacga cagcagccgc cacactgggc
     2881 atgggacgca gcacatggag cgaaaacagt gcaaatgcaa aaaccgacag cattagtctg
     2941 tttgcaggca tacggcacga tgcgggcgat atcggctatc tcaaaggcct gttctcctac
     3001 ggacgctaca aaaacagcat cagccgcagc accggtgcgg acgaacatgc ggaaggcagc
     3061 gtcaacggca cgctgatgca gctgggcgca ctgggcggtg tcaacgttcc gtttgccgca
     3121 acgggagatt tgacggtcga aggcggtctg cgctacgacc tgctcaaaca ggatgcattc
     3181 gccgaaaaag gcagtgcttt gggctggagc ggcaacagcc tcactgaagg cacactggtc
     3241 ggactcgcgg gtctgaagct gtcgcaaccc ttgagcgata aagccgtcct gtttgcaacg
     3301 gcgggcgtgg aacgcgacct gaacggacgc gactacacgg taacgggcgg ctttaccggc
     3361 gcgactgcag caaccggcaa gacgggggca cgcaatatgc cgcacacccg cctggttgcc
     3421 ggtctgggcg cggatgtcga attcggcaac ggctggaacg gcttggcacg ttacagctac
     3481 gccggttcca aacagtacgg caaccacagc ggacgagtcg gcgtaggcta ccggttctga
     3541 cggacaggaa gcagacagcc gcaaagatca cggtctttgc ggctgtttct tatgaaaaga
     3601 aaaccctatt ccaattgcct gcttctattg tttcaagact tcttccaaag attcggcatt
     3661 aatcagatgt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
     3721 aaatagtacg gaaccgattc actcggtgct tgagcacctt agagaatcgt ttctttgagc
     3781 taag
//
LOCUS       DAWGJV010000159         3742 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWGJV010000159 DAWGJV010000000
VERSION     DAWGJV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.159"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            148..1665
                     /locus_tag="WHQ94_001820"
     CDS             148..1665
                     /locus_tag="WHQ94_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2129056.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRAQALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1726..3507)
                     /locus_tag="WHQ94_001821"
     CDS             complement(1726..3507)
                     /locus_tag="WHQ94_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ2129057.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          775 a          837 c         1247 g          883 t
ORIGIN      
        1 gtgaaatccg ccctttgaaa atcgggcggc ggttttttgc ctgcccgctt tcagacggca
       61 aagccggttt tcacgggttt ctgcctgttt ttcggatggt ttgacgtgct tcggcggcgt
      121 gtttgccgga aaggtaaatg acagggtatg ttgtatttca gatacggctt tttggttgtt
      181 tggtgtgcgg caggtgtttc tgccgcctat ggggcggatg cgcccgcgat tttggatgac
      241 aaggcattgt tgcaggtgca gcggtcggtg tcggataagt gggcggaatc ggattggaaa
      301 gttgaaaatg atgccccgcg cgtggttgac ggggattttt tgttggcgca cccgaaaatg
      361 ttggaacata gtttgcgcga cgcgctcaac ggcaatcagg cggatttaat cgcttcgttg
      421 gcggatttgt atgccaagct gccggattat gatgcagttt tgtacggcag ggcgcaggct
      481 ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcggtatcg gaagctgcac
      541 ggggaaaatg cggcagacga gcggattttg ctggatttgg cggcggcgga gtttgacgat
      601 ttccggctga agtcggcaga aaggcatttt gcggaggcgg caaaattgga tttgccggcg
      661 ccggttttgg aaaatgtggg gcgttttcgg aaaaaaacgg aggggctgac gggctggcgt
      721 ttttcgggcg gcatcagccc ggcggtcaat agaaatgcca ataatgccgc gccgcagtat
      781 tgccggcaaa acggaggccg gcagatatgc agtgtcagcc gggcggagcg ggcggcaggg
      841 ttgaattatg aaatcgaggc ggaaaaactg acggcgttgg cagataatca ttatttgttg
      901 ttccgttcca atatcggcgg cacgagctat tatttcagta aaaaatcagc ttatgatgac
      961 gggttcggca gggcgtattt gggttggcag tataaaaatg cgcggcagac ggcggggatt
     1021 ttgccgtttt atcaggtgca gttgtcgggc agcgacggct ttgatgcgaa aacaaaacgg
     1081 gtaaacaacc gccgcctgtc gccgtatatg ctggcgcacg gagtcggcgt gcagctgtcc
     1141 catacttacc gcccaaaccc gggatggcaa ttttcggtcg cgctggaaca ttaccgccaa
     1201 cgctaccgcg aacaggatag ggcggaatac aataacggcc ggcaggacgg gttttatgtt
     1261 tcgtcggcaa aacgtttggg cgaatcggca actgtgttcg gcggctggca gtttgtgcgg
     1321 tttgtgccga aacgcgaaac ggtgggcggc gcggtcaata atgccgccta ccggcgcaac
     1381 ggtgtttatg ccggttgggc gcaggagtgg cggcagttgg gcggtttgaa cagtcgggtt
     1441 tccgcgtctt atgcccgccg caactataag ggcgttgcgg ctttctcgac agaggcgcaa
     1501 cgcaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc gtacaaaggt
     1561 atcgtgcccg cgttgaatta tcgtttcggc aggacggaaa gtaatgtgcc gtatgcgaaa
     1621 cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt gggataatgc
     1681 cgtccgaact ttgtggaaac aggttcggac ggcatttttg cgcgttcagg caagggcggc
     1741 ggcaaatacg ccgcgcaagg cgttggagag gcggatttct tcggcttctt gcagtgtttt
     1801 ttgtgtgatg tgtgtttcga ttacttgatt tgtttgcaaa tatttttgcg gttcgtccaa
     1861 cacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg tgagccattg
     1921 tccgcgatgt ttgacgaaca cgttgcttct gccgccttcg agcaggatgc cgtctgaatt
     1981 gaaaaacagg ctgtcgaacg cgccttgtgt ttcggcggtt tgccacgctt ggtcgaagac
     2041 ggtgcggcag gtggttttga agcggcgcag gtagttttgt gcgggcagga tggtcggcga
     2101 aatgatgacg cgttgtttgt cggtcagacg gtttaaaacg gcgcgggaca ggctgatgcc
     2161 gtctgaagcg agcagggctt tgatgcggaa cgcgccgtcg ggcaagcgtg cgatgtattg
     2221 tttgatttga ttttcgcaac cgtcgggcag gggcaggttg agggcttggg cggaggtttt
     2281 cagacggcat aggtggcggt cgagcagggc gcagcgtccg ttttccacgc gcagggtttc
     2341 aaaaatgccg aagtcggggc gcaattcgtt gaggaaacgg gctttccagc cgcattcccg
     2401 atattcggcg gcagggtcgc tgtcgatgac gatgccggaa ccgacaccgt acacgccttg
     2461 ataaatgccg tctgaaagcg gcgtgagcga caaggtgcgg ataacgacgt tgaacgtgcc
     2521 ttcaaacccc aagccgccgg aacacgggtt caaatagccg atgctgcccg tataaagtcc
     2581 gcgcgcttcg gcttcgagcg attcgataat ctgcatactc atttttttgg gcgcgccggt
     2641 gatgctgccg caggggaagg cggcgcggag gatgtcggcg aacgaggtgt gcggcaaggc
     2701 ttgggcttgg atggtgctgg tcatctgcca aacgctgccg aaacgcgata ctttaaacgg
     2761 ttcgggtacg catactgtgc cggtttgggc gattttgccg aggtcgttac gcagcaaatc
     2821 gacaatcatc acgttttcgg cgcggttttt cgggtctgct tgcaactcgg cggcgcggcg
     2881 ttcgtcttgt ccgtcgccca aaatcggcgc ggtgcctttc atcggttcgg tgctgatggt
     2941 gccgtccgaa ccgattttga ggaagagttc gggcgagaaa cacagcgtcc acgcggattg
     3001 cccttgtgca tcgggcaggt gggacaaaac ggcatagggg acgggctggc gcaggcgttg
     3061 gtaaagtttg acgggattgc cgtaggcttg caggtgcagg cgggtagtgt agttgatttg
     3121 ataggtgtcg ccgcgtcgga tggcttcgtg gatttggcgg atatggtcga ggtaatcggc
     3181 ttcggatacg gaggattgcg gcgtggaaat gccggcgggg aggccgtctg agtgtcgggc
     3241 aagccagctt gcggcatcgg tgtcggcgca gtcggcaaac cagtgcaggg caaggttgcc
     3301 gccgcgttcg gactcgaccc ccgtcagcgg caaaccgaat ccgtagtctg caaacaacac
     3361 ggcatgcagc cctttttgcc agcccgattg cagcgcgccg tccaaagcat cgagttcttc
     3421 gggacggaaa aaacggcttt ccacatgatt ttgatagcgt tttgcgcggc cgcttacggc
     3481 atcgtcaaac agggcgaaat aaggcatggc aattcggggc aaatgttttg attatacgct
     3541 ctttttacac atattttcag acgattcggc aaatatcggc aaaatgtaat tttatgtaga
     3601 gaaagtgggg ggcgaaggtg taaaattgtg aaaacaaatt ccgatttcca acctgaaata
     3661 caataaggag acctttatga cagaccacca gctgcaaccg tttgaaaacg tagaattagg
     3721 cgaaaagcaa gatcagctcc aa
//
LOCUS       DAWGJV010000160         3735 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWGJV010000160 DAWGJV010000000
VERSION     DAWGJV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.160"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..380)
                     /locus_tag="WHQ94_001822"
     CDS             complement(<1..380)
                     /locus_tag="WHQ94_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129058.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WHQ94_001823"
     CDS             complement(438..803)
                     /locus_tag="WHQ94_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129059.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1306)
                     /locus_tag="WHQ94_001824"
     CDS             complement(785..1306)
                     /locus_tag="WHQ94_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129060.1"
                     /translation="MILTMIAAPAMANSMDNQTFENQVFHTRADAPMQLAELSQKEMK
                     ETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPGGVGAAGKV
                     VSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGIGRHRPW
                     ESKSTDRSWKNRF"
     gene            complement(1495..1767)
                     /locus_tag="WHQ94_001825"
     CDS             complement(1495..1767)
                     /locus_tag="WHQ94_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129061.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILASVSDEDGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2329..2801)
                     /locus_tag="WHQ94_001826"
                     /pseudo
     CDS             complement(2329..2801)
                     /locus_tag="WHQ94_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2890..3114)
                     /locus_tag="WHQ94_001827"
     CDS             complement(2890..3114)
                     /locus_tag="WHQ94_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129062.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
     gene            complement(3384..3677)
                     /locus_tag="WHQ94_001828"
     CDS             complement(3384..3677)
                     /locus_tag="WHQ94_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129063.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFVVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT         1196 a          802 c          565 g         1172 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcaccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccccctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat gtctgattgt
     1261 ccatgctgtt tgccatcgcc ggagcggcaa tcattgtcag aatcatcatc gctgcggtaa
     1321 tttgtttttc ataataactc ctttggatta caaggttgga aaatcaaagc cctgcttaga
     1381 acgtatgttg cacacccaat ttcaataggt aaatcagatt gcaaatccag caatttgaat
     1441 attgtcattg ttccgtgcaa aagggatttt tattgatgag ttgtgtactg ggtttcagct
     1501 tggcttttta gataatcttt tcacaagaac atacatcaat actgtccaaa aaaaaagatt
     1561 tgataaatat attgcaacat attttattcc atcttcatcc gaaacagaag ccaatatatt
     1621 tttaattaat acatagccga ttattacatt tattattcct ataatatata aggatcttgc
     1681 caaaattttt tttgatttta ttttaggaat atctcttatc catgctaaaa tacagcccaa
     1741 tgtcgaaaag aaaataacag caaacactac cttacctcct ctcacttcca accaaaccga
     1801 tagcaggttt ggttggaagt tggtttattt attatttaac ggcgaatgtc agtgttctta
     1861 cccgtagaac ctgcataacc agttatgcca ccagcaacag tccccactgc tacacctcgc
     1921 ataccattaa tcagaattct tcctattttt tcgaatccac tatttcttcc aaagatgcag
     1981 caaacgcagg ttgagcctag acccaatcta cgtttgctgc cgcatctgat ccctattgtt
     2041 tcttatcctt acatcttcct gccttgtcaa tcaaataaag acagaagact atacaaaaac
     2101 tgaccgacat actgaaaata cctatcccct tccatgcaat ctccgcacca ttgacccaat
     2161 agattagact gagaaacaac aaagccacag tataaatcaa cgtaaaaaat attgcgtaaa
     2221 gcacaataaa gggaactttt atctcacggt tgttttttat aatatattca gcaacttgat
     2281 ttccgaatat acctgataaa aaatatagta ttagatcatc catttcgttt atcttctatg
     2341 ttttcccatt gcggcgctag aagactgatt taatgctgca ccatttgcac gaataactgc
     2401 attaggaata gcatttcccc ctttccaagc agaacgggca aaaacactag tagtaatccc
     2461 tgcaccgcgc aacatcgcac tgctatatcc gccgcctatc ataccaccag cggttgcagc
     2521 caaggtactt cttgttgaag caaattgccc tgttttaatt ttagaaatgc cgtgattagc
     2581 ccaagcacta attgcccctc ccatcaaagc acctgccaca ataggaagaa acgccccctc
     2641 agtctccttc atctcctttt gagaaagctc cgccaactgc atcggcgcat ctgcccgcgt
     2701 gtggaacact tggtcttcaa atgcctgatt gtccaagccg tttgccattg cggggcgatt
     2761 atagacagca tcatcgtggc ttcagtgatt tgttttttca taacaaattt ttagggagga
     2821 gataatgttc catcaaaatc ctcctgaaag gctgcctgaa atttccaagc agcctttttg
     2881 attgcttaat taatcggata gtgcgacaaa tataaagtgg gcggcaatta ttgccgtaac
     2941 ggcagccata actgttttat tttcttcaaa ccaaagcaag ttgagaaaca taaacatcaa
     3001 gaaatatgat ggttcttcag ttttgccgct gctttcttga gttagaagat aaaatataga
     3061 tatggcggtt atgccgattg ctgcttttat taaaatatat ttgtttttca tcatcttgta
     3121 tggtttttca tgcaagtttc aacaataaaa ttcggaataa gatagagaga tgttgatatt
     3181 tcagataaat atacaaaaca atcaagaaac tattggcagt atgtataatc ttgaatttct
     3241 ttttttgatg aaattaaaat aatgaaatat atggtaaacg ttacaacgga agccttagcc
     3301 tcccgattgc agtatttcaa ttccaagata aatgccgcat agaagataaa atactcaaat
     3361 ggttttttag cggcttaaat atcttattta atcttattga ttgttttccc aatggtttcc
     3421 gcaataaata ataagaagta gaataataaa caaacagaac atgaaaataa aaataaaaat
     3481 aaatctttca ttatcccagt aataaaactc caaagatttt ccttatttcc atcaacaata
     3541 aaaattatca gttcatgaat aaagaatact acaaagaaaa tgcttcctgc cgttagagat
     3601 atttctaatc tgcttatttt cccttcgcta tatttacctg ataaatagcc taaaaaagct
     3661 gcaatcaaat catccatttt aaaaatttct gtaaaaacag gtaatgtctt taattcatta
     3721 cctgttttgc catta
//
LOCUS       DAWGJV010000161         3655 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWGJV010000161 DAWGJV010000000
VERSION     DAWGJV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.161"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(119..274)
                     /gene="rpmG"
                     /locus_tag="WHQ94_001829"
     CDS             complement(119..274)
                     /gene="rpmG"
                     /locus_tag="WHQ94_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ2129064.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(306..539)
                     /gene="rpmB"
                     /locus_tag="WHQ94_001830"
     CDS             complement(306..539)
                     /gene="rpmB"
                     /locus_tag="WHQ94_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ2129065.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(789..2315)
                     /gene="farB"
                     /locus_tag="WHQ94_001831"
     CDS             complement(789..2315)
                     /gene="farB"
                     /locus_tag="WHQ94_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ2129066.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKADMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(2339..3493)
                     /gene="farA"
                     /locus_tag="WHQ94_001832"
     CDS             complement(2339..3493)
                     /gene="farA"
                     /locus_tag="WHQ94_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ2129067.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVQQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGDVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT          894 a         1019 c          919 g          823 t
ORIGIN      
        1 tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaaaga
       61 aacacgataa aaataaaaaa gcctccgaac agtcggaggc tttgctttca aactgaaatt
      121 atttcagttt ggtttctttg tacactacgt gtttgcgggc aaccggatcg aattttttaa
      181 tttccagttt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac
      241 ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat taaatagtgt
      301 ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat gcctactttg
      361 tcgatggtac gcagtgcagc gttggaaacg cgcaggcgaa cccagcggtt ttcactttct
      421 acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt gttgttggcg
      481 tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac tcgtgccata
      541 atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa acagtgttag
      601 ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga ttgtcgcatc
      661 tgcggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt tcgggaactg
      721 ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat ttcaagtttt
      781 caaatccctc aatgtccacc gccgccactg ctgtggaacg gcggttttgc cagccatatg
      841 acgggtatca tgataatgaa taacaagctg cctgccataa agatttcatt cgatccgatg
      901 atgaagccct gctgggtaat ggtattgttg atgataccta gggtttgaat gtcggaaatg
      961 ccttgttggg acaaatgcgc cgccgtttcg tgcaatgttg cggaataggg cgtgatgtgt
     1021 tcggcaaagc gcgtgtggtg caacgcttcg cgccgttccc acagggtgct gacgacggat
     1081 acgccgacac cgcccatcag cacgcgcagg aaattcgaca ggctgccggc ggcggcaatc
     1141 tgcccgccct tcatgtgcga cagtgtgatg gtagtcagcg gcaggaagaa catggcaacg
     1201 cctactccct gccaaaactg cggccagatg acgttgccga tatccatgtc ggcataaaaa
     1261 tcggtgcgcc aatagaaagt gaaggcaaag gtcaggaagc tggcggttac gagcaggcgc
     1321 atatcggcct tgttgccgaa tctgccgata atgggggaca ggaaaactgg gaggatgccg
     1381 acgggcgcgg cggcaagccc tgcccacgtg gaggtatagc ccaggttggt ctgcaacact
     1441 aacggcagca gggtcagcgt ccccatatac accataaaac ccaatgacgt ggcaatgacg
     1501 ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata tttttcgccc
     1561 aattcccaaa caataaaata cgacaagcac accaatgcgg ttacgcccaa ggtaatgatt
     1621 tctcccgaag cgaaccagtc gagttcctta cccctgtcca acatcatctg taacgcgccg
     1681 ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac ggtttccgtt
     1741 tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat agggatatta
     1801 atgaagaaaa tccaacccca atgccagttt ccggaaatcc agccgccgag tatcggcccg
     1861 agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt ccgttttgcg
     1921 ggcggatagg atgccattaa caggctttgc gacaggggaa tcagcggccc ggcgataaaa
     1981 ccctgcaaga tgcggaaaaa caccaaagcc tgaaggttgg gcgcgatgcc gcacagccat
     2041 gatgtgatga cgaaaccgac agcggcggcg gtaaacagtt tgacctcgcc gatacgtttt
     2101 gccaaaaagc ccgttaacgg cacggaaacg gcgtttgcca cagcaaaaga agtaatgacc
     2161 cacgtcccct gcgtggttgc cgcaccgagg ttgccggcga tgacgggaac ggcgacattg
     2221 gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag cgtaacccac
     2281 gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc
     2341 agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac
     2401 tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag
     2461 tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc acatcttcgc
     2521 ggttcagcac gatacggacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt
     2581 tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaccct gccaccctgc
     2641 cgcgataaac aatttgtttg ccgtacaaat cggacaccag ttcggcaggc tgtccgattt
     2701 tcatatgccg caactgcgtc tctttaaaat tggcatccac ccacacatcc gacagcggca
     2761 ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg
     2821 ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac cacgcatctt
     2881 tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaaca tctccgccca
     2941 aagccgcgcg tgccgaagat tcttccgcca aagccgcttt gaccgccgcc tgcgcctgag
     3001 acactgccgc acgggcgtgt gccagctctt cggcggacac cgcgcccgat tccgccaaag
     3061 cagaccggcg gcgcaaatca tcctgtgcgc gtgccaaatc cgcccggcgc aaggcaacct
     3121 gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgctgcacc gcctgaacca
     3181 gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat accgccagca
     3241 cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc gtaccgccct
     3301 tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt
     3361 gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg aacagcagcg
     3421 tcaatgccgt caggcggcgt ttgcgtttgg cttgcgtgtt ttgaagtttt gtttcgtccg
     3481 tgtgcgtatc cataagattg ggttccgaat ttcctgattt catataaaca atgttctttc
     3541 aataggaagc agattctata tccgcgcaat aacgcaatca agtcagtatt ttagatgcaa
     3601 tattcaaaaa acaacgcttc aggcggcaaa actgaaaata tatttcgttt atata
//
LOCUS       DAWGJV010000162         3601 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWGJV010000162 DAWGJV010000000
VERSION     DAWGJV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.162"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            160..1281
                     /gene="dnaJ"
                     /locus_tag="WHQ94_001833"
     CDS             160..1281
                     /gene="dnaJ"
                     /locus_tag="WHQ94_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ2129068.1"
                     /translation="MSNQDFYATLGVARTATDEEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYESCDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKAVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLNLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIIVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1477..3495
                     /locus_tag="WHQ94_001834"
     CDS             1477..3495
                     /locus_tag="WHQ94_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ2129069.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3539..>3601)
                     /locus_tag="WHQ94_001835"
     CDS             complement(3539..>3601)
                     /locus_tag="WHQ94_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ2129070.1"
                     /translation="KDLAGKTWAQAEKLRLTLSR"
BASE COUNT          783 a          825 c         1057 g          936 t
ORIGIN      
        1 tttcaatttc cggacgggta aagccgccga tacccggcgc attgttaaag gaacaacaga
       61 agaaatgccg tctgaaaggc ggtttttgtt cgggaaacct tgtgttttgt cctgttgccc
      121 ttattttatc gacgatttta ttttgaatca gatgaaaaga tgagcaatca agatttttat
      181 gccaccctag gtgtggcaag aacagctacc gatgaagaaa ttaaaaaagc ctaccgtaaa
      241 ttggcgatga aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt
      301 aaagaagtac aaaaggcgta tgaaactttg tccgataaag aaaagcgcgc tatgtacgac
      361 cagtatggcc atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggagga
      421 ttcggcggtg ctcaaggttt tgactttggg gatattttca gccaaatgtt tggaggcggt
      481 tcggggcgcg cccagcctga ttatcagggc gaggacgttc aagtcggtat cgaaatcacg
      541 cttgaagaag cagcaaaagg cgtgaagaaa cgcatcaaca ttccgactta tgaatcgtgc
      601 gatgtctgca acggcagcgg tgcgaaaccg gggacatccc cggaaacctg cccgacttgc
      661 aaaggttcgg gaacggtgca tatccagcag gcgattttcc gtatgcagca gacttgtccg
      721 acctgccacg gtgccggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg
      781 cggaataagg ccgtcaaggc ggtggaagtc aatattcccg ccggtatcga tgacgggcag
      841 cgtatccgtt tgagcggcga aggcgggccg ggtatgcacg gcgcgcccgc aggcgatttg
      901 tatgtaaccg tccgcatccg ggcgcataag attttccaac gcgacggctt gaatttgcat
      961 tgcgaactgc cgatcagttt tgccacggcg gctctgggcg gagagttgga agtgccgacc
     1021 ttggacggta aagtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgc
     1081 gtgaagggta agggagtcaa atctttacgc agcagcgcga ccggcgattt gtactgccat
     1141 attatcgtcg aaacacctgt taatttgacc gaccgtcaaa aagagctttt agaagaattt
     1201 gaacggattt ctaccgggtt ggaaaatcaa acaccgcgta agaaatcgtt tttagacaag
     1261 ctgcgcgatt tgtttgattg attttgaggc ttgtaaacag gcggccgtat cggggaatct
     1321 ccctgatacg actgtttttg tttgcttgaa aatagttttt attttcaagg gagtacgaga
     1381 taggattgga taactgtttt taactgttct ttttaaaact tgacattatg acatattgcc
     1441 aacaatatgc gggaatctgt tgtcaaagga agaataatga ataaatcttt atccggttcg
     1501 gtagaagaat accgcgaact gacgctccga ggcatgatac tcggtgcatt gatcactgta
     1561 atttttactg cgtccaacgt ttacctcggt ttgaaagtcg ggctgacctt tgcctcgtcg
     1621 attccggcgg cggtgatttc gatggcggtt ttaaagtttt tcaaaggcag caatattttg
     1681 gaaaacaaca tggtgcagac ccaagcctcg gctgcgggta cgctttcaac catcatcttc
     1741 gtcctgcccg gtttgctgat ggcgggctac tggagcggtt tcccgttctg gcagacgacg
     1801 cttttatgta ttgccggcgg gattttgggg gtgattttca ccattccgct gcgttacgcg
     1861 atggtggtga aaagcgattt gccttatccg gaaggtgtgg cggctgctga aattttgaaa
     1921 gtgggcggtc atgaagaagg ggataaccgt cagggcggca gcggcatcaa agagctggcg
     1981 gccggcggtg cgttggcggg attgatgagc ttttgcgccg gaggtctgcg tgtgattgcc
     2041 gatagcgcga gttattggtt taaaagcggt acggcaattt tccaactgcc gatgggcttt
     2101 tcactggcat tgttgggcgc gggctatttg gtcggactga cgggcggtat cgccattctg
     2161 ttgggcattt cgattgcttg gggcattgcc gtgccgtatt tttcatcgca catcccgcag
     2221 ccttccgata tagaaatggc ggcgtttgcg atgaagctgt ggaaggaaaa agtacgcttc
     2281 atcggcgcgg gaaccatagg tatcgcggcg gtttggacgc ttttgatgct gctcaagcca
     2341 atggtggaag gcatgaagat gtcgttcaag agttttggcg gcggtgcgcc cgctacagaa
     2401 cgcgctgaac aggatttgtc gcctaaagcc atgattttct gggtattgtc tatgatgttt
     2461 attctaggcg tgtcgtttta ccactttatc ggcgattcgc acattacggg cggcatggct
     2521 tggcttttgg tggtcgtttg cacgcttttg gcttccgtca tcggcttttt ggtcgccgcc
     2581 gcctgcggtt atatggcggg tttggtcggc tcgtcttcca gcccgatttc cggcgtgggc
     2641 atcgtttcca tcgtcgtgat ttcgctggtt ttgctgctgg tgggcgaatc cggcggtttg
     2701 atggcggatg aggcaaaccg caaatttttg ctggcactga ctttattttg cggctcggca
     2761 gtaatctgcg tggcttcgat ctccaatgac aacctgcaag acctgaaaac cggctacctg
     2821 ctcaaagcca cgccttggcg gcagcaagtc gccctgatta tcggctgtat cgtcggtgcg
     2881 ctggttattt cacccgtgtt ggaactgctt tacgaagcct acggctttac cggcgcaatg
     2941 ccgcgcgaag gtatggacgc ggcgcaggct ttggcagccc ctcaagcgac tttgatgacg
     3001 accatcgcgt cgggcatttt cgcccacaac ctcgaatggg tctatatctt taccggcatc
     3061 gcgattggcg cggtgttgat tgccgccgat ttggtgttga aaaaatcatc aggcggcaaa
     3121 cttgccctgc ccgtccttgc ggtcggtatg ggtatttatc tgccgccgtc cgtcaatatg
     3181 cccatcgtgg caggcgcggt gttggcggcg gtgttgaaac acatcatcgg taaaaaagcg
     3241 gaaaaccgtg aaggccgtct gaaaaacgcc gagcgcatcg gaaccttgtt ctccgccggc
     3301 ctgattgtcg gtgaaagcct gatcggtgtg attatggcgt ttattattgc cttctccgtg
     3361 accaacggcg gctcggatgc gccgctcgcg ttgaatctgc aaaactggga tgccgccgct
     3421 tcttggttgg gtttggcgtt cttcgttacc gggatgtttt tctttgcaca gcgcgtactg
     3481 aaggcgggta ggtagcctgt cggagaaaat gccgtctgaa acgttcagac ggcatttttt
     3541 atcgggaaag tgtaaggcgg agcttttcgg cttgcgccca cgttttgccg gcaaggtctt
     3601 t
//
LOCUS       DAWGJV010000163         3502 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWGJV010000163 DAWGJV010000000
VERSION     DAWGJV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.163"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            43..711
                     /locus_tag="WHQ94_001836"
     CDS             43..711
                     /locus_tag="WHQ94_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ2129071.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKEQGMLAFGDKNGNK
                     IYDGDVTDVLLRSVVLNDDINDKRINYAFNHIAFGSSQPTADRVVWTFNQNGTFGYTK
                     DQHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNNRRTV
                     CQYK"
     gene            741..1355
                     /gene="pilV"
                     /locus_tag="WHQ94_001837"
     CDS             741..1355
                     /gene="pilV"
                     /locus_tag="WHQ94_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ2129072.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFAIDA
                     MKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSLGAAPTLSAGSTFSQNCDGSA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1352..2338
                     /locus_tag="WHQ94_001838"
     CDS             1352..2338
                     /locus_tag="WHQ94_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ2129073.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNRS
                     FSLKRNGIDKLIPIAESSNIGYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQ
                     WGNPQLLVKKINKMDIRYIYLLVEKPSTPPTNPPQYIYGCPEDEDTGKEEKFKYTDKF
                     DSSKDAVTPAGAEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2317..2916
                     /locus_tag="WHQ94_001839"
     CDS             2317..2916
                     /locus_tag="WHQ94_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ2129074.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNNGNEEVFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGTEYKKGTQSVSKMPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2921..3394
                     /locus_tag="WHQ94_001840"
     CDS             2921..3394
                     /locus_tag="WHQ94_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ2129075.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQFILKNPLDDNQTIKSKLEIFVSRYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSADAGCEAFSNRKK"
BASE COUNT         1015 a          705 c          869 g          913 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctctagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacgccca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataagat atatgacggc
      361 gatgtgacgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggttcgtctc agccgaccgc cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaagc
      541 aaatccagct ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattaacag cagcggcagg
      661 gttgaggttt gtcctagaaa caataggcgt accgtatgcc aatataaatg attgttttag
      721 tcggtttggg aaataaaaaa atgaagaata atgattgctt ccgcctgaaa aacccccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg aaggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatcataca ctatcagttg
     1021 tggatggcga ttttgcgatt gatgccatga aaactaagac gcagttggca gaggcacaat
     1081 tgaagagatt tagttatgag ctgaaaaatg ccttgccgga tgcggcagcc atccattacg
     1141 ccgtctgcaa ggattcgttg ggtgctgcgc cgacattgtc cgccggcagt actttttctc
     1201 aaaattgcga tggtagtgca aatggggata ctttgattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatc gcccgtacga atcttgagac gaacggcaac aatatcgtat
     1321 atacctatca ggcaagggtt ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcctgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acatcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ctattcaaca ggatttgcgg aatgcggcaa cattaattgt
     1561 ccgcgatgca agaatggcag ggggcttcgg ttgtttcaat atgtccgagc atcctgcaac
     1621 tgatgttgtt tccgatgtgg cgcaaaaaaa ccgttctttt tccttaaaaa ggaacggtat
     1681 agataaactt attcccatag cggaatcttc aaatatcgga tatccgggtt ttacccagcg
     1741 ccttaacgca ttgattttcc aatacggaat cgatgatgtt aatgcaagcg ccgatactac
     1801 cgtcgtcagc agctgtgcca aaatagcaaa accgggtaag aaaatatcta ccttgcaaga
     1861 agcaaagagt gcattacaga ttactaatga tgataaacaa aatggaaata tcacccgtca
     1921 aaggcatgtg gtcaatgcct atgcggtcgg caggattgcc ggtgaggaag gtttgttccg
     1981 cttccaattg aatgatgatg ggcagtgggg caatcctcag ttgttggtta aaaagattaa
     2041 taaaatggat atacggtata tctatcttct cgtggagaag cccagtacac cgcctaccaa
     2101 tcctccccaa tatatttacg gctgccctga agatgaagat accggcaaag aggaaaaatt
     2161 caaatatacg gataaattcg acagctccaa agatgctgtt acgcccgccg gggcggaggt
     2221 tttattgagt agcggtactg ataccaagat tgccgcttct tcagacaatc atatttatgc
     2281 ttaccgtatc gatgcgacaa tacgcggggg aaatgtatgc gcaaacagaa cactttgacg
     2341 ggaatcccga cttctgacgg acagaggggg tttgcactgt ttatcgtgct gatggtgatg
     2401 atcgtcgtgg cttttttggt tgtaactgcc gcgcagtctt acaataccga gcagcggatc
     2461 agtgccaacg aatcagacag gaaattggct ttgtctttgg ccgaggcggc tttgcgggaa
     2521 ggcgaacttc aggttttgga tttggaatat gatacggaca gtaaggttac atttagcgaa
     2581 aactgtgaaa aaggtctgtg taccgcagtg aatgtgcgga caaatacaaa taatggtaat
     2641 gaagaggttt ttgacaatat cgtggtgcaa ggcaagccca ccgttgaggc ggtgaagcgt
     2701 tcttgcactg caaattctac caacctgtgc attgacaata aagggacgga atataagaaa
     2761 ggcacgcaaa gcgtcagcaa aatgccacgt tatattatcg aatatttggg cgtgaagaac
     2821 ggagaaaatg tttatcgggt tactgccaag gcttggggta agaatgccaa taccgtggtc
     2881 gtccttcaat cttatgtaag caataatgat gagtaataaa atggaacaaa aagggtttac
     2941 attgattgag atgatgatag tcgtcgcgat actcggcatt atcagcgtca ttgccatacc
     3001 ttcttatcaa agttatattg aaaaaggcta tcagtcccag ctttatacgg agatggtcgg
     3061 tatcaacaat actgtcaaac agtttatttt gaaaaatccc ctggacgata atcagaccat
     3121 caagagcaaa ctggaaatat ttgtctcacg ctataagatg aatccgaaaa ttgccgaaaa
     3181 atataatgtt tcggtgcatt ttgtcaataa ggaaaaacca agggcataca gcttggtcgg
     3241 cgttccaaag acggggacgg gttatacttt gtcggtatgg atgaacagcg tgggcgacgg
     3301 atacaaatgc cgtgatgccg cttctgcccg agcccatttg gagaccttgt ccgcagatgc
     3361 cggctgtgaa gccttctcta atcgtaaaaa ataaggttgt tttgccgatg ccgtctgaag
     3421 attaattttc agacggcatt tttatgggta tagtggatta acaaaaatca ggacaaggcg
     3481 acgaagccga agacagtaca ga
//
LOCUS       DAWGJV010000164         3485 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWGJV010000164 DAWGJV010000000
VERSION     DAWGJV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.164"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WHQ94_001841"
     CDS             complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WHQ94_001841"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ2129076.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMARHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSTVERQWFQNLLDLGLTDSLRHIHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRVDLDTRALERPSDHAPVTAEFDW"
     gene            complement(1860..2141)
                     /locus_tag="WHQ94_001842"
     CDS             complement(1860..2141)
                     /locus_tag="WHQ94_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ2129077.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLSRLRVEGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WHQ94_001843"
     CDS             complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WHQ94_001843"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ2129078.1"
                     /translation="MPSEKTLFPLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
BASE COUNT          846 a          822 c          873 g          944 t
ORIGIN      
        1 cgtttcatga aactgatgca gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
       61 ccgcagacag tacagatagt acggcaacgc tgtactggtt taaatttaat ccactatatt
      121 ttgtaatagc tgccattatg cctactagga tatatacctt ctatataatc cttatttaaa
      181 taaaaaccct tataaaaatc tgtgttatct gtcgggattg aaaaaaagat tttatctttc
      241 tcatcaggtt tatatttgct atcaatatca aaaagagaat aaggattaca atgagttcga
      301 catactaggt aaaaatttat atcttggatc atgaaatatt cgatatttat caggatcgaa
      361 agatgctaaa aattcatcaa tattggtact ggatattttg tcatcactaa taaacatcgc
      421 tagagatggg cgactttgat tttccatttg ttgattagta aaggatataa atactaactt
      481 gccattgtca aaccacatag atttatatag tggattaaat ttaaaccagt acgtcgttgc
      541 ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
      601 aatccactat agttattgcc tttgggagaa tcaattctga cagcacataa catagattta
      661 tctttacagt cgttatattg ggtttcatca aactcaactg catggcacaa taaaggcaat
      721 aggaaaaaca gggttattaa tttattacta tatataagac ggctttctat ttattcgaaa
      781 ataaatctag ttttcaatat ttgattttgt tttggattag tgtacataat aaattttact
      841 gctccaaaat ctgatattaa ccctatgtat ttcataaatt tttcaggaaa ataggctgtg
      901 tttgaacaat ccgccgattt cggctgtttg tcagggaaaa ggcaattatt ttcggttagt
      961 cagcagccta tcctattgaa tcacttaaag atatggatgt ttgtcaacgc catatcaaaa
     1021 cacggtcttt taccaatcga attctgccgt caccggcgcg tggtcgctcg gacgctctaa
     1081 agcgcgtgta tccaaatcga cacggacatc cttcaaaacg gccgccattt cggatgtaac
     1141 caatatatgg tcgatacgca ggcccagttt gcgctggaac atcgcgccgc gatagtcgaa
     1201 ccatgtgtaa aacgcacctt cgggatggat atggcgcaaa ctgtcggtca atcccaaatc
     1261 cagcaggttt tgaaaccact gccgttcgac ggtcgaacag tggatttttt cataccattt
     1321 ttcagggtcg taacagtccg catcggcagg cgcgatattg aaatcgccca acagcaccag
     1381 tttgccgtgg cgtgccattt catcgcggac aaattcggtt aacgcggcaa accattgttc
     1441 tttatatttg aatttcgggc tgtcgagggc ttcgccgttg acgcaataga cattgatgac
     1501 gcgcacgccg ccgacggttg ccgcaatcac gcgccgttgc ggatcgtccg gcagcgcggg
     1561 cagcccgata tgcacgtctt ccggcgcgtt gcggctgacg attgccacgc cgttgtaggt
     1621 tttctgcccg ctccaaacac agtgccagcc catcatctgc aaggcggcag ccggaaattt
     1681 gtcctgatcg agtttgagtt cctgcaaaac caaaatatcg ggcggattgt cggcaagcag
     1741 gttttgcacc tgcggcagcc gcacattgag cgaattgacg ttccaagtgg tgattttcat
     1801 aacatttccg aaaaatgccg tctgaaacgt tcagacggca aaaggaaagg attttaacac
     1861 tataccgcgc gtttgttttc caaatcgcgg attgtccgca gaatcgttgc cgcgtcttcg
     1921 gaaatcaggc ggtattcgat aatgcggtgg taacgcgtaa agtccaccag accttccacg
     1981 cgcagtcggc tcaaatggcc ggaaacggcg gtggccggca gggataagga ttttgccagt
     2041 tcggcgatat tgcgttcgct gtccaacagt tggatcagta tcgccatacg ttcgggatgc
     2101 gccataagtt tgagtatggt gtggagcggt attgtgttca tcggtattcc taaaaccttt
     2161 gccgcccgcc aagcggattg acgggcgaca gatatggatg attgtgatta tctttataaa
     2221 tcttattata aatcaaatat ttatggatga ttgtacagga ttgtatgttc cttttcccac
     2281 tttttttgca aacacgcgta aaggcgggct ttcagacggc ataaaactgc cgccccccgt
     2341 cctttttcct gccctaccga tgccgccgcg ctgtcgggca aacaaaatac ctgcctgaaa
     2401 tgctgttttt tctaaagcat cacgaccttt acgaaaaatg ccgtctgaag aatctttcag
     2461 acggcatttc ctccctgagg caataaccgg attagggtta agcggttcct tgcctgacat
     2521 atcagacagc cgcccgcacc ctaaaactca cgccacgaaa tccaaaccta tgtccaacga
     2581 acggctgctg tgggtcagat agccgagggc gatgccgtcc acgccggttt gcgccacgcg
     2641 cttcaggcgg tcgaagccga tgccgcccga tgcttcgcaa tagatggtgt gggggtgggc
     2701 ggtttgcgtg tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttatccag
     2761 caaaatccgt tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tatccacttc
     2821 gatttccacg caggtcaatg ctccgacagc ctgttttgcc tgctgcacgg cttgggcgat
     2881 gctgccgcaa taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca aacccatgcg
     2941 gtggttcacg ccgccgcctg ccctgacggc gtatttttgc aggacacgca gcagggggat
     3001 ggttttgcgg ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc
     3061 ggtggcggtg gcgatgccgc ttaagtgcgt gaggtagttg agcgcggtgc gttcggcggc
     3121 gagcagcgcg cgggcgttgc cttcgacggc ggcaagcgtc tgacctgcgc ggacggcttg
     3181 cccgtctcgg atttcggctt ggaagcggac ggacggatcc atcgtctgaa aggcgagacg
     3241 cgccaagtcc atgccggcga taacgccgtc ttcgcggctg acaaggaaga gtttggcggt
     3301 tttgtcgggg gcgatgacgg cggcggacgt aatatcgccg cgcctgccca agtcttcgct
     3361 caaggcttgt tctactatgg ggcgcaacag ggtgtcgggc aggggaaaga gggttttttc
     3421 agacggcatg gggtttcctt tgttgaaaaa gattgatgtt gcaccgatgg ggcaaaactt
     3481 cattt
//
LOCUS       DAWGJV010000165         3477 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWGJV010000165 DAWGJV010000000
VERSION     DAWGJV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.165"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..159
                     /locus_tag="WHQ94_001844"
     CDS             <1..159
                     /locus_tag="WHQ94_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129079.1"
                     /translation="ALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKT
                     VYLPGLHP"
     gene            171..680
                     /locus_tag="WHQ94_001845"
     CDS             171..680
                     /locus_tag="WHQ94_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129080.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(733..1080)
                     /locus_tag="WHQ94_001846"
     CDS             complement(733..1080)
                     /locus_tag="WHQ94_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2129081.1"
                     /translation="MYIPDKGDIFHLNFDPSGGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSGGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1082..1318)
                     /locus_tag="WHQ94_001847"
     CDS             complement(1082..1318)
                     /locus_tag="WHQ94_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ2129082.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1677..2168)
                     /locus_tag="WHQ94_001848"
     CDS             complement(1677..2168)
                     /locus_tag="WHQ94_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ2129083.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACEIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2420..2590)
                     /locus_tag="WHQ94_001849"
     CDS             complement(2420..2590)
                     /locus_tag="WHQ94_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129084.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSSPA
                     FKQERMSRMPDF"
     gene            complement(2606..2974)
                     /locus_tag="WHQ94_001850"
     CDS             complement(2606..2974)
                     /locus_tag="WHQ94_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ2129085.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          889 a          843 c          798 g          947 t
ORIGIN      
        1 gcgctgaaat tcgaccagtt gatactcgaa tttcccgagc gcggggacgg cgcatgggta
       61 cacatcggtt tccgacgcaa cagcccgcaa cgcaaccaga tactgaccgc aaccaagaaa
      121 aacggcaaaa ccgtgtatct gcccgggctg catccttgag gtcatctgaa atggatattt
      181 tattgaaata ctggaagccg gtaggcgtat tgctgctgat agtcctgatt tttaccgcat
      241 ggcatttcga ccgtgccgaa aaataccgca tgggacggga ggctgctgct gccgaaattt
      301 cgaatcgtct gaaagacggc tatatcgagc aggcaaagca ggcgcgttct gccgagcaga
      361 aggccgctgc cgcgtttgcc gaacgacaaa ccaaattaga agaggaaaaa caaaatgctg
      421 aaaaaactgt tgccgctatg cgtcttgagc ttaaccgcct gcgccactac gccgccgccc
      481 aaaatcgcag cagaaacctg cccgcaacca ctaccgccgc caccgcatct gatggcgcgt
      541 cagattccca aggctggcta ttattcggac agtgcgctga aaaatatgca ggactggcag
      601 aaacagccga cggacatgca gccgatttaa gggaatggca ggcatatggt catgcagtaa
      661 gcagtcagcg tgccgaataa gcaaccgccc gaacctgtaa gaaaagatta caggttcggg
      721 cggtttcagc atttaatcga ataagacggc gccgatgcgc gccagcacat cgtccaatac
      781 ataatcgggt acagtttctt taaaagaagc cttgcggatt tgccagtcca gagatttgag
      841 ctggtggcag tggacattgc cctgcgtttc cgttcctgca ccgagtaagg ttgaaatcat
      901 gccgccgctt cgtgcagccg ctgcattccc ctgtgaaatg gggcaggcaa aaaccaatcc
      961 cgttgcgcgg ttgaatgctt ttggagacag agccagcgca aaccgcccgc ccttgatttc
     1021 cttgccgccg gaagggtcga aattcaaatg gaaaatatcg cctttgtcgg gaatatacat
     1081 ttcagacgac ctcgttgccg acatcatcca agatttccca gccttctacg cgcggcgggg
     1141 tttcttccat ttcggcaagc aagtctgcca agcggaaacg tcgggcagca cgcacacgga
     1201 gttcgccgtt atgtacttcc gctaccaaag cgtcgccgat tttaaaatcc aattgtttca
     1261 gcatgtcggc aggcagtcgg acggccgccg agttccccca tttttggaca cgcaacataa
     1321 tcttcacctt tattgtatct acaaagtaga tacatattac cataaaattt cagttgttca
     1381 aatacttgtg cagaatacgc caaaagccgt ccgaactgtt tcggacggct tttgtactgt
     1441 atttgcgcct tcaggcaata ttttgttatc cattttcaag atgcaaaagc tttctaattg
     1501 cttgatgtcg gatttcggtt gtttagggat acaaaaccaa gtaaactaaa actgtttgat
     1561 tggaaaatgc tccgcaagga gaaaattatg ttcaaaaaat cactttataa ggctgctttg
     1621 gcgtatttcg gcgattgcgt ggctgcccat atttcagaac agttttgact gtttattcac
     1681 aaatcagatg cctttagggg gtttgctttc cataatgcaa ccaaaatttc caactctcta
     1741 aatattgtgt ctttgcgttc ttcttcgcgt attttcataa caagaggcga aactgcattc
     1801 cacaatttta tgaagttggt gcaatgcagc cgtttaaaca aatcctcatc caatactccc
     1861 gagttgattt cgcacgcata aaactcgtgc cgattgatta cggtaagtaa atgccctctc
     1921 tctttttccc attgtccgcc cttattttga taaatttcat acaggtcgat ccgcgcgttg
     1981 ttgtcgtcaa ctgccgtgcc gcgaatataa ggcgaaatat gattgtcggc ttctacaaat
     2041 tgtgcatctt ggtaatcatt caaaataaca tctaatgtcg ccttttgctt tgaagttttc
     2101 ttattgatga atattgtccc cagtgcaatg acggcggtta ctgaaacaac ggtcagttgc
     2161 caaaacatca gccattcggc cgtccctcat aggttaaact gtacgctgtg cctgaatatg
     2221 taaaataaag tgaatacaga tacaaacaag gaggttaggg caattactga caaccaacct
     2281 ccttgtttta tttgtttccg caccttacca gccattaaaa ccctcatttt tgtatttcat
     2341 cgtatttcct taatggttgt aaccttgctt gtcgatatct gtatattaat gccgtgcgcc
     2401 cgttctttca aggggtgatt taaaaatcag gcatccttga catcctctcc tgcttaaagg
     2461 cgggggaact cctgccgtgt aaaccaatgc cgtctgaagg gctttcagac ggcataaaaa
     2521 aaccgccttt gtgtaaagcg gttgaagaaa agcctttcaa taaaaatgcc gtctgaattt
     2581 cagacggcat tgttgtcgga tatgcctatt ccttatccag tcggcgcagg gtttcggcga
     2641 gctgtttgaa gtcggtttcc cccgccgcca aactgacaat caagtcgtca agcccttggt
     2701 cggcggcaat gctgatgccc tgcaaatcca gataggtcaa cattgttaaa agcgcggtgc
     2761 gcttgttgcc gtcgggaaag gcgtgggctt tggctatggc ttgtgcataa agggcggcga
     2821 tttcgtagat gtcctcaagg ttttcatact gccgccagtt ggcaatccgc gacaatgcgc
     2881 cgtcgagccg cgccatatcc gcccgccctt tcaaacccgc ttcatccgcc aatacggttt
     2941 gatggataag cgcgaccagt tcgccgtcta tcatttgtcg gcaagtgcct tgacggcttt
     3001 ttgatgggtt ttggcaatgc ggcgggcggc ggcaagcagt acgcgtttgc ctgcttcgcc
     3061 tttaagctcg attttgacgg ggcgtgtatt ttggttttgc atcgctgctc cttgtctgtt
     3121 tgcgggcatt ttagcttttt tccggcagct tgggaaatgc cgtctgaaaa cacttcagac
     3181 ggcattcttc taatagtgta atgcaatagt tactccaaga tttttgtata tggctgtttt
     3241 tatggtaaat taaattttca caaagaataa atatatttaa atcaataaaa taaaatttag
     3301 tcgaatccca ccgtttccgc cagaaaacaa aaccgccctg attcagggcg gttctttttg
     3361 gcgcgaatct gcacaattac cataaaagaa tcattggatt atgcttattt tccgtaacgc
     3421 cctgcaataa accgcaatcg gcagatgctt tgatcacagt tttatggtaa tttatat
//
LOCUS       DAWGJV010000166         3453 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWGJV010000166 DAWGJV010000000
VERSION     DAWGJV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.166"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            186..1061
                     /locus_tag="WHQ94_001851"
     CDS             186..1061
                     /locus_tag="WHQ94_001851"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ2129086.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARKLNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1070..2266
                     /gene="bamC"
                     /locus_tag="WHQ94_001852"
     CDS             1070..2266
                     /gene="bamC"
                     /locus_tag="WHQ94_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ2129087.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2323..3255)
                     /gene="pip"
                     /locus_tag="WHQ94_001853"
     CDS             complement(2323..3255)
                     /gene="pip"
                     /locus_tag="WHQ94_001853"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ2129088.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDILPRLL"
BASE COUNT          912 a         1139 c          776 g          626 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc agaagcatta ccgcccccta caccacaact gataaaaggt
      181 tctgtatgtt acaaggtagc ctggttgccc tgattacccc gatgaatcaa gacggcagca
      241 tccattacga acaactccgc gatttgatcg actggcacat tgaaaacggc acggacggca
      301 tcgttgccgt cggcacgaca ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg
      361 tcatcgaagc cgtcgtcaaa cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg
      421 caaacaacac cgtcgaagcc atcgcccttt cccaggccgc cgaaaaagcc ggtgcagact
      481 tcactctctc cgtcgtcccc tattacaaca agccctccca agaaggcatt taccaacatt
      541 tcaaaaccat cgccgaagcc acttcgattc cgatgattat ctacaacgtg cccggccgta
      601 ccgtcgtcag catgactaac gacactatcc tgcgccttgc cgaaatcccc aacatcgtcg
      661 gcgtgaaaga agccagcggc aacatcggca gcaacatcga actcatcaac cgcgcgcccg
      721 aaggcttcgt cgtcctttcc ggcgacgacc ataccgccct gccgttcatg ctgtgcggcg
      781 gacacggcgt gattaccgta gccgccaacg ccgcacccaa gctctttgcc gatatgtgcc
      841 gcgccgcgct gcaaggcgac attgccctcg cacgcaagct caatgaccgg ctgattccga
      901 tttacgacac catgttctgc gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc
      961 tgggcagatg cgaaccgcac gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg
     1021 caaaagtccg cgccgccctg aaagcctcag gacaactcta aatataacta tgccgtctga
     1081 accgttcgga cggcataacg caacaaacac tttaatatcc atcacacagg atgacacgat
     1141 gacccttatc aaacccgtca ttgccgcgct cgcactcatc gggcttgccg cctgctccgg
     1201 cagcaaaacc gaacagccca agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact
     1261 tgaagtccca cccgatttga acaaccccga ccaaggcagc ctctaccgcc tgcctgccgg
     1321 ttcgggagcc gtccgcgcca gcgatttgga aaaacgccgc acacccgccg tccaacagcc
     1381 tgccgatgcc gaagtattga aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca
     1441 acgttggctt gtcgttgacg gcaaatcccc tgccgaaatc tggccgcttc tgaaagcctt
     1501 ttggcaggaa aacggcttcg acatcaaatc cgaagaaccc gccatcggac aaatggaaac
     1561 cgagtgggcg gaaaaccgtg ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac
     1621 agtcggtttg ggcggcatct actccaccgg cgagcgcgac aaattcatcg tccgtatcga
     1681 acagggcaaa aacggcgttt ccgacatctt cttcgcccac aaagccatga aagaagtgta
     1741 cggcggcaaa gacaaagaca cgaccgtatg gcagccctcc ccgtccgatc ccaacctcga
     1801 agccgctttc ctgacgcgct ttatgcaata tttgggcgtt gacggacagc aggcggaaaa
     1861 cgcatcggca aaaaacccga ccctgcccgc cgccaacgaa atggcgcgta tcgaaggcaa
     1921 aagcctgatt gtctttggcg actacggcag aaactggcgg cgcaccgcgc tcgccctcga
     1981 ccgcatcggg ctgaccgtcg tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa
     2041 agccccgaac gaaagcaatg cagttaccga acaaaaaccc ggcctgttca aacgcctgct
     2101 gggcaaaggc aaagcggaga aacctgccga acagccggaa ctgattgtct atgccgagcc
     2161 tgtcgccaac ggctcgcgca tcgtcctgct caacaaagac ggcagcgcat atgccggcaa
     2221 agacgcatcc gcattattgg gcaaactcca ttccgaactg cgttaaacca ttcaaaacaa
     2281 aagcagcttc caaacagaag ctgctttttt atgcggaact ttttacaaca aacggggcaa
     2341 aatatcctca accgcctgaa ccagcgcatc cgccaaaggc ggatcgaacg cacgatgccc
     2401 tgcctgaacc accctcaatt ccgcttccgg aaaggctttc gacagcgccc acgcactctg
     2461 catcggcgta cacaaatcat accgcccctg cacgataatg gtcgggatat gccgtatttt
     2521 gccgatattg ttcaaaatcg ccctatcgcc ctgcaaccag ccgccgttga caaaataatg
     2581 gttttccaaa cgcgcgattg ccagcgaggc ataagcatct tcatccactt cctccggctc
     2641 gaaacggatc agatagcttt cccaatccgc ccaagccttt gcggcagaca ggcacacttc
     2701 ttcatcttga tgaaacagca acccgtgata cgcctcaatc agccggttcc gtcgattttc
     2761 agcaatcggc gcgacaaatt tttgccattg ttccggataa atccggctca caccgcccgc
     2821 ctcgttcagc cacgccgttt cagacggcct gcacaaaaat atcccgcgca ataccaatcc
     2881 ctttacccgt tcaggatggg tttgggcata agccagcgac aaagtgctgc cccacgaacc
     2941 gccgaacacc agccatttcc tgatacccag catttcacgg actttttcaa tatccgccac
     3001 caaatcccaa gtcgtattgt cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg
     3061 ctggtcgatg atgacgatgc ggaacacatc gggattgaaa aaaccccgac attcaggcga
     3121 agcccccgcg cccggcccgc catgtaaaaa aataaccggc acaccgtcgg gattgcccga
     3181 ttcctcccaa taaatttgat gaatttcaga cacctgcaag tatccgctat gaaaaggctg
     3241 ttttatctca tacatcatct tctcccatat tgcttctgac aatccgaaca ttcccacacg
     3301 caacatcacg cacgagacac aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata
     3361 gtgaattaac aaaaatcagg acaaggcggc gagccgcaga cagtacggat agtacggaac
     3421 cgattcactc gtgcttaagc accttagaga atc
//
LOCUS       DAWGJV010000167         3414 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWGJV010000167 DAWGJV010000000
VERSION     DAWGJV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.167"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(214..1278)
                     /locus_tag="WHQ94_001854"
     CDS             complement(214..1278)
                     /locus_tag="WHQ94_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ2129089.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEDIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHIADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WHQ94_001855"
     CDS             complement(1279..2037)
                     /locus_tag="WHQ94_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ2129090.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WHQ94_001856"
     tRNA            complement(2112..2187)
                     /locus_tag="WHQ94_001856"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..>3414)
                     /locus_tag="WHQ94_001857"
     CDS             complement(2241..>3414)
                     /locus_tag="WHQ94_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ2129091.1"
                     /translation="FGIRQNASCENIVDWHERFPELEAQLGAPIEDYAPPKHWTRKQL
                     RSMGRVSYLCVDAAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTG
                     TSRNFSANTYVRMMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLT
                     DMMLAGGGEEFFSSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLE
                     ELEHAKRRGAIIYAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGW
                     INLHGTGTHHNDSMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQ
                     SNPEGKLPPQLWDGQNDPNLPAINLTGSGSRWETEKRIAASSSFTFGGSNCVLIIG"
BASE COUNT          772 a          837 c          903 g          902 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc ggcttatatt gcatccaata atttgtcggc
      241 gatatggttg atggtaaaac ggttggcgac gcattgatgc cctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcatcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cgcccaaagt
      421 attggaaatc actgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgtcttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtgataa aaatcggttt tgttggcgac gacattgtgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc gaataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc attgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaacccc caaattgggc ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg aaatggaagt
     1501 atatttgttg aatttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttag ggtagggggt ctgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgcct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc gcccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcatcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt catttctagc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggta
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gaaggttcgg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttgcgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgagaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagtccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacgtgcc ggtcagcaac
     3121 aattcgccca catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atccgtccgt cggtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaa
//
LOCUS       DAWGJV010000168         3353 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWGJV010000168 DAWGJV010000000
VERSION     DAWGJV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.168"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WHQ94_001858"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WHQ94_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ2129092.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="WHQ94_001859"
     CDS             2186..3070
                     /locus_tag="WHQ94_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129093.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
     gene            3082..3300
                     /locus_tag="WHQ94_001860"
     CDS             3082..3300
                     /locus_tag="WHQ94_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129094.1"
                     /translation="MCGSTTHAVPNRPKPRACLRLTPYHPTNTASVGWILVSDKTFNI
                     PIIAIHCSNAQNVGFENPTYKIIPSIIL"
BASE COUNT         1027 a          882 c          689 g          755 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt cattcgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccgact tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccaa gccgcgattt gttcatcgaa aaagacagtg
      481 cgatcaacga acacattgaa caaatgcgcc tttctgccac caaaaacctg atgacgcgcg
      541 atgacgtgat tatcgttgcc actgtgtccg ccatttacgg tatcggcgac ccgactgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgacaccat cgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga tttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt tgacccgctt tcaggcagcc
      841 tgcaccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccacccccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgaccct caaatcatca tccgccccgt cgccacccaa gttgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgtactcgtt accaccctta
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttacggctcg
     1621 gattgtttga cgtactcgtc ggcatcaatc tcttgcgcga aggcctcgac atcccagaag
     1681 tctccctcgt cgccatcctc gacgcagaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggcgaa attcacaacg aagaagacgc gattaaagaa atcgccaaac
     2041 tcgaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggcatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccacac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcccaaaatg tcggattcga gaatccgacc tacaaaatca ttccgagcat aatactatga
     3301 aataccgtcg tttttaccgc aatggcggca cttacttttt tacggttgta gcc
//
LOCUS       DAWGJV010000169         3339 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWGJV010000169 DAWGJV010000000
VERSION     DAWGJV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.169"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            62..691
                     /locus_tag="WHQ94_001861"
     CDS             62..691
                     /locus_tag="WHQ94_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ2129095.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            866..3238
                     /gene="hmbR"
                     /locus_tag="WHQ94_001862"
     CDS             866..3238
                     /gene="hmbR"
                     /locus_tag="WHQ94_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ2129096.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAATETTPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDSSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTKV
                     AAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTRFKRFTLRLDSHPLQLGGRHRLS
                     FKTFVSRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSRA
                     GIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWHVGYDITSGY
                     RVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRNF
                     LSEEQKLTTSGTPGCTEESAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVDK
                     VASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTYL
                     GAKKAKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGVY
                     NVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          908 a          859 c          831 g          741 t
ORIGIN      
        1 gcgggcaagt gcggattttg cccgcaccgc atccttatcc cttaccgaca aaggaaaaaa
       61 tatgagtgaa accgaaaatc aagcattgac atttgccaaa cgcttgaagg cggataccac
      121 ggcggttcac gacagcgtgg ataacctcgt tatgtctgtc caaccgtttg tcagcaaaga
      181 aaactacatc aaatttttga aactccaatc cgttttccac aaggctgtcg accacatcta
      241 taaagatgcc gaattaaaca aagccattcc cgagctggaa tacatggcgc gatacgatgc
      301 cgtaacgcaa gaccttgcag atttgggtga caaaccttac gaatacggca aaccgctgcc
      361 gcatgaaacc ggcaacaaag caatcggctg gctttattgc gccgaaggat ccaatttggg
      421 cgcggcgttt ttgttcaaac acgcccaaaa actcgattac aacggcgaac acggcgcgcg
      481 ccacctcgct ccgcatcccg acgggcgcgg caaacactgg cgtgccttcg tcgagcatct
      541 gaacgctttg aacctgactc ccgaagccga agcggaagcc atccaaggcg cgcgcgaagc
      601 ctttgcattc tacaaagtcg tgttgcgcga aaccttcggt ttggcagccg atgccgaagc
      661 accggaagga atgatgccgc acaggcacta aaaaataatc gaaccaaata aacaaggtct
      721 cggcatagct gtttgcaggg acctttaatt acacggcgcg gctttgttta catggattac
      781 tgtcttatta aatattaatg attatcataa aatctattat tcgctaaccg atggatgaac
      841 aatccataca tcttgagttg ataatatgaa accattacaa atgctcccta tcgccgcgct
      901 ggtcggcagt attttcggca atccggtctt ggcagcagat gaagctgcaa ctgaaaccac
      961 acccgttaag gcagagataa aagcagtgcg cgttaaaggt cagcgcaatg cgcctgcggc
     1021 tgtggaacgc gtcaacctta accgtatcaa acaagaaatg atacgcgaca ataaagactt
     1081 ggtgcgctat tccaccgatg tcggcttgag cgacagcggc cgccatcaaa aaggctttgc
     1141 tgttcgcggc gtggaaggca accgtgtcgg cgtgagcata gacggtgtaa acctgcctga
     1201 ttctgaagaa aactcgctgt acgcccgtta tggcaacttc aacagctcgc gtttgtctat
     1261 cgaccccgaa ctcgtgcgca acatcgaaat cgtgaagggc gcagactctt tcaataccgg
     1321 cagtggtgca ttgggcggcg gtgtgaatta ccaaacgctg caaggccgtg atttgctgtt
     1381 ggacgacagg caattcggcg tgatgatgaa aaacggttac agcacgcgta accgtgaatg
     1441 gacaaatact ctcggtttcg gtgtgagtaa cgaccgcgtg gatgctgctt tgctgtattc
     1501 gcaacgtcgc ggtcatgaaa ccgaaagcgc gggaaaccga ggctatgctg tggaagggga
     1561 aggcagtggc gcgaatatcc gtggttcggc acgcggtatc cctgattcgt ccaaacacaa
     1621 ataccacagc tttttgggta agattgctta ccaaattaac gataaccacc gcatcggcgc
     1681 atcgcttaac ggccagcagg gacataatta cacggttgaa gagtcttata acctgaccgc
     1741 ttcttcctgg cgcgaagccg atgacgtaaa cagacggcgc aatgccaacc tcttttacga
     1801 atggatgcct gattcaaatt ggttgtcgtc tttgaaggcg gacttcgatt atcagaaaac
     1861 caaagtggcg gcggttaaca acaaaggctc gttcccgatg gattattcca cctggacgcg
     1921 caactataat cagaaggatt tggacgaaat atacaaccgc agcatggaca cccgattcaa
     1981 acgttttact ttgcgtttgg acagccatcc gttgcaactc ggggggcgac accgcctgtc
     2041 gtttaaaact ttcgtcagcc gccgtgattt tgaaaaccta aaccgcgacg attattactt
     2101 cagcggccgt gttgttcgaa ccaccagcag tatccagcat ccggtgaaaa ccaccaacta
     2161 cggtttctca ctgtctgacc aaattcaatg gaacgacgtg ttcagtagcc gcgcaggtat
     2221 ccgttacgac cacaccaaaa tgacgcctca ggaattgaat gccgagtgtc atgcttgtga
     2281 caaaacacca cctgcagcca acacttataa aggctggagc ggttttgtcg gcttggcggc
     2341 gcaactgaat caggcttggc atgtcggtta cgacattact tccggctacc gcgtccccaa
     2401 tgcgtccgaa gtgtatttca cctacaacca cggttcgggt aattggctgc ctaatcccaa
     2461 cctgaaagcc gagcgcagca ccacccacac cctgtctctg caaggccgca gcgaaaaagg
     2521 catgctggat gccaacctgt atcaaagcaa ttaccgcaat ttcctgtctg aagagcagaa
     2581 gctgaccacc agcggcactc ccggctgtac tgaggaaagt gcttactacg gtatatgcag
     2641 cgacccctac aaagaaaaac tggattggca gatgaaaaat atcgacaagg ccagaatccg
     2701 cggtatcgag ctgacaggcc gtctgaatgt ggacaaagta gcgtcttttg ttcctgaggg
     2761 ttggaaactg ttcggctcgc tgggttatgc gaaaagcaaa ctgtcgggcg acaacagcct
     2821 gctgtccaca cagccgctga aagtgattgc cggtatcgac tatgaaagtc cgagcgaaaa
     2881 atggggcgta ttctcccgcc tgacctatct gggcgcgaaa aaggccaaag atgcgcagta
     2941 caccgtttat gaaaacaagg gctggggtac gcctttgcag aaaaaggtaa aagattaccc
     3001 gtggctgaac aagtcggctt atgtgtttga tatgtacggc ttctacaaac cggctaaaaa
     3061 cctgactttg cgtgcaggcg tatataatgt gttcaaccgc aaatacacca cttgggattc
     3121 cctgcgcggc ctgtatagct acagcaccac caactcggtc gaccgcgatg gcaaaggctt
     3181 agaccgctac cgcgccccaa gccgtaatta cgccgtatcg ctggaatgga agttttaatc
     3241 tggtattatt gaattaatcg ccttgttgaa aattaaagcc gtccgaattg tgttcaagaa
     3301 ctcattcgga cggcttttta ccgtaaatct gtgtgatgg
//
LOCUS       DAWGJV010000170         3281 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWGJV010000170 DAWGJV010000000
VERSION     DAWGJV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.170"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(180..563)
                     /locus_tag="WHQ94_001863"
     CDS             complement(180..563)
                     /locus_tag="WHQ94_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129097.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAKYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(568..1075)
                     /locus_tag="WHQ94_001864"
                     /pseudo
     CDS             complement(568..1075)
                     /locus_tag="WHQ94_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(1704..2099)
                     /locus_tag="WHQ94_001865"
     CDS             complement(1704..2099)
                     /locus_tag="WHQ94_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129098.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2111..2824)
                     /locus_tag="WHQ94_001866"
                     /pseudo
     CDS             complement(2111..2824)
                     /locus_tag="WHQ94_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2920..3192)
                     /locus_tag="WHQ94_001867"
     CDS             complement(2920..3192)
                     /locus_tag="WHQ94_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129099.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
BASE COUNT          958 a          666 c          537 g         1120 t
ORIGIN      
        1 tgtgagcttt tcagacggca ttgagccgta aatcatggaa cgcgtgcgtg ctggagcaca
       61 caccttacgc atggatttta ggtttcatgc aggctacagc ttgctgctgc ggtataaaag
      121 tatttttagt gatttactgt ttttggtatt tttgcccgac ggggttaaaa atacagttgc
      181 tactcttgct gcatgatatt ggcaagattt aatttaaatg tttgttgaat cttgtttaca
      241 tccttagcaa aaatatgata gtcactcgaa gaatagatag attttattct atccaaatta
      301 gtttgttgat aacatttttt catttccgcg atgaaaaaat tatcttttcc tctttcattg
      361 agacagttat tttctaacag atagattgcc catgctgtaa aaataaatgt actatcttcc
      421 ggatattcag ataaataaat ttcgaattca attatagaat atttagctgc ttcaattaat
      481 gttatatcta aatccctgac aaatttttcc gttatatttt taaataattt cttgaattct
      541 atataatttt ttattttttc catgattcta ttttcctgta aaaatattta attgaatatt
      601 tggatagcga tttctaaact ctaaaataac attgctgcaa gattgacagg cctttaattc
      661 tgtaaataga tcaattctac ctgatacatt acgattattt ccgagttgct gtgctatagt
      721 ttcaagtaat ttatattctc catctatatt tcttaatgtt cctctaggag gagaggcaat
      781 attatgatat ttatcaacag atataggttt aaaaattttt gtttcaggta acgaaataaa
      841 tccatgttcc cctttttgga aagaactaaa tgcttccatc ctttgcggta atcctttaac
      901 atcaattttt gctatcgcaa gattccctat ttgtcgatac tcttccggca gtttcatgcg
      961 gactctctgg ctttcagcag ttaaaagcag gagcactgtc attgctcaga taacgggtaa
     1021 caggtgtagg cttggccgat attgcagcct gtgcagatac agatcctgca accatcaatg
     1081 tgccattaac aacctgaact gtaccatgca gatattctcc attagagatt gctttcgctc
     1141 cctgtccaat gttatatccc cctgctgcca cagtagctgc tttgacactg aagccaagcg
     1201 gtccgtttaa taagtaaagt gcaggttgtg cggcaacagc tgatttgtaa tcaaaagaat
     1261 cataacgact caatgtttga taggctttcg ataaagtgtt attaatatct ttggaaggaa
     1321 tagtgagatt acccattgac tggggcgttt tcagtatcga agctgcccaa gctgtgttac
     1381 ctccagaggc ttggttaata aaatatgtta cctatgtata ttgtttcttc aaaaatcaac
     1441 cgtattgtcc ttgggtgatt ttcgtaccag cacaatcagt ctgccctagt tgacagatcg
     1501 cgccacacta gccagataca gcgtatcatt ggtaaaactg ataatgcggc aatataaaga
     1561 aaaacttcaa tagtatgtta acgaaatttg acgcataaaa atacgctaaa aaattttcgc
     1621 ttgactaatt aaaaatggga ttgagcataa aattttaata atttatatta ttgaaaatat
     1681 ctcagatttt cagtcagttt attttacaag ccgaaccgta ggctattttc catctttaac
     1741 catgaataga cctcaacttc atcataattt gtgcctgcaa tagcaacttc cagaaagtca
     1801 ataattctac ctgctttatt caaagactct gtagtatata gcaggaaagt cgctgcatct
     1861 gacttatccg gtgcagtgat gtctccttca aaaaaaatag ggatattttc gtatttattt
     1921 agattagttc tttctaaatt tacctgaaaa tcaatgtagc acccacatcc tgtattatct
     1981 ctagatacta ttattgaatt ttctatttgc tctattaaat attccttcca tatcatattg
     2041 ttttcaataa ttctacataa gaaagccttc tcatattcag ataaacaaaa ctggttcata
     2101 tattctaccc ttacattttc aaaatagttg caatacgaga tactccattg ttgacatata
     2161 cgcgaacaat taccgttata ccattgaccg ttacagtaac tggataattt cctgtttttg
     2221 tcagttttaa agcatccact gccttttgcg tcgctacaac agcattttcc atggagcctg
     2281 atttttgaat caatgtatta ccgatgcttc cttgatgccc cttaaagatg tgatgaactt
     2341 tatttagcca atctacaccg tatctttcag ccaatagtcg agaattactt tcacctattt
     2401 tttccatcga agctaaaatc ctttgagcat aggcagcatc agctgttgaa aaatttgctt
     2461 cgcccaacaa aagccgtaat tcctgaatat ctcctcgtaa aattgcattt cttactcccg
     2521 cttctaatgt ggtttgtggc tgaaagaggg aattattctc taccgccaca gtagccgcat
     2581 tcgccgctgt attcacatcc ccgcctacaa cggcagacgc agtgccggca accagttttg
     2641 aataggcagt aatcttcgct ttagcttttt cgatttcggt cgcactcata cgactgaaat
     2701 cagtattctt aaccaaagcc tcaccaacaa tctcaccgac tgcagcgcct atcgcaccat
     2761 cctgacactt acccttattc gccgccgctg ccgcacagcc cgctatggca tgggcaatct
     2821 tgtgggtggt atatcgctgc cagccctctc tgttgataat cttgcgtatg attacgccgt
     2881 ttgatggcta atcgtaccta cgcaggctta aaatttccat caatccatat catttgagtt
     2941 ttcatcttca taatatggat gatcaaacaa atctccttcc caaatacatt cactcgttat
     3001 accttgggat tccatgaaag gaacataaga ataaaaatct ttttcatatt ctccataaaa
     3061 tatattcata cctttgagcc atactgcatc gcattcatca cacagaataa tttcatcatg
     3121 aagatattta ggtttcgaat ggtagagata gccttgttga caagcaggac acattttcat
     3181 tctcttattc atgattactc catataatcg atttattttt gtgggtaagc agtagtgatt
     3241 tgattgctgc ctttagtctg tacaacaata cgtatatagc a
//
LOCUS       DAWGJV010000171         3254 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWGJV010000171 DAWGJV010000000
VERSION     DAWGJV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.171"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WHQ94_001868"
     CDS             complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WHQ94_001868"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ2129100.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1370..3248)
                     /locus_tag="WHQ94_001869"
                     /pseudo
     CDS             complement(1370..3248)
                     /locus_tag="WHQ94_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
BASE COUNT          754 a          792 c          976 g          732 t
ORIGIN      
        1 cggcaatcgg attatttcca aaccaaaagc gcgtgattgc gtttgccgcg ccgaaggatg
       61 gtgtatttgc cgaaacgttt gtgttcgtcg gtcagcaggc aggcatcgtc ggggcgttcg
      121 gcggcgtggt tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg
      181 ttcacaaagc cgcgcgcttc tttattggag gatgccaaac cggttttgac caaggcttcg
      241 acgacattga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg
      301 aagtcgcttt cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgctgc
      361 gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt
      421 tgcgcttcgg gcttgctgcc gcttgcctgg tctttggctt cgacggcatc gatttcttcg
      481 atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc
      541 cagaattggt agaactgata gggcgaggtt tttttcgcgt tcagccatac tgcgccgcct
      601 tcggttttgc cgaatttggt gccgtctgat ttggtaacca aaggcagggt caggccgaac
      661 acttgttttt gattcaggcg gcgggtcagg tcgataccgg cggtgatgtt gccccattgg
      721 tcggagccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtag
      781 ccttgcagca gggagtaggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcaatg
      841 cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg
      901 cgcaggaagt cgaggcagtt cattttgccg aaccagtcgg cgttgttcgc cataatggcg
      961 gcgtttccgc cttcaaagct caagaaaggg gttaattggc tgcggatgct ttccacccag
     1021 ccggcaacgg tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg
     1081 atcataccgg tcgcaccgcc caccagtgca atcggcgtat gccccgcctg ttggaagcgg
     1141 cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag
     1201 ccgcaataaa gggaaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg
     1261 gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa
     1321 aaaaaattag cggttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat
     1381 ggcgcgtatc gagaaaccgt tgtttttcgg aaaaacgctt cgccaattcc gtgccgccgt
     1441 aagggttgat gtggtctttg tccgagtaaa ccggcaatcc gccgatttgg aaatttgcgg
     1501 ggatataggc ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga
     1561 tgcgtgcatt ggctttcaga gtgctttcgt cgtccgggcg cagggtttgg cggtaacccg
     1621 gtatgcgtga agacaagata taggcgcgct ggacgttgta agacgaggca aggttgtccg
     1681 ccatcaggta aacggcttgt ttttcggacg agagtttatg cagcatacgg tcgaattttt
     1741 tgaaaaaacc ggcatcgtag gcaagggagc ggctgttttc gggcatttgg ctgccccacc
     1801 gcatcgccaa aaccactttt gaataccggg gcaggtgttc ttcggcatag cggtaaacgg
     1861 cgcggcaggc ggcccagttt tggaatacgc gggacgcgta gccttccaca taggcgcagg
     1921 cgtcggcgga aaccatagtg gcagcccatt tttctttttt gcccacggca tcgaagaatg
     1981 ttttgtaatg gtcggcgtgg gagtcgccca aaaccagcag ttccggctgt ttttccgtat
     2041 ccccccatag gcattgtttg ccggtattgt tgtggcagga ggtgttggaa cgcgtcagcc
     2101 ccaagcggtc gtattgcgcc ataaacggca gcctcatcgc aaaaaatgag cccgccccca
     2161 aaatgagcat aggcaaggca taaatccata aaacggattg tgcgaacgaa ccttgccatt
     2221 ttttaaacgg tttttcgatg cagtggtaag aaaacaggga aagcagcaat atcaggacga
     2281 ccgccgccgc cggcgaataa ggcggcaggt tgtccgggcc gatatagcgc ataaaggcca
     2341 atatcggcca atgccacaga taaagcgaat aggaaatcaa accggcggca acggtgattt
     2401 tccattggaa aaatttttta agcgggtgtt cgtaatgatt gaaataaatc agcgcggcaa
     2461 cagccagaca gggaatcaaa gcggcgggcc ctgggaaata ggcggtttgt tccgaatagg
     2521 caaacaggca ggcggacagt atgcacgcgg aaaacaatgc gccggcggcg gcacagcatc
     2581 tgccgacggc gagattccgc tgttgccggt accgcatcca cacggcggtc agcgatccga
     2641 ccagcatttc gcaggcgcgc aggtggggca ggtaatattt atcgaacgcg gaaggcataa
     2701 aggaagcggc aaggcttaag gcgcacaatg cggcgaggaa gccgaactgt acgcgcgggc
     2761 ttttgcgggc gacaagcagc agcaatatcg gaaagacaaa gtaaaattgt tcttcgaccg
     2821 acaaagacca aatgtgcagc aggggctttt cttcctgcgc gggatcgaaa taatccttcc
     2881 cccttgcaaa atacaggtta gaggcgaaac ccaaggcggt cagcgcggat ttccacaaaa
     2941 gaaagaaatc atctttggtg aataaaaaga agccgcctgc cagcgttgcc gccaatacgg
     3001 cgaaaaatgc gggcagaatc cgcttgatgc ggcggatata aaatgccttc agggaaaacc
     3061 tccccccccc ccgacatttc gcggtgaaga atcgtcgtca tcaaaaagcc tgaaatcaca
     3121 aagaatatat cgacaccgag aaacccgccc ggcagccaat ccttttcgat atggaacacg
     3181 atgacggaca agacggcggc ggcgcgcaat gtgtcgatgt ccgggcggta gggtaaggct
     3241 tggctcataa tgtt
//
LOCUS       DAWGJV010000172         3215 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWGJV010000172 DAWGJV010000000
VERSION     DAWGJV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.172"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            160..711
                     /locus_tag="WHQ94_001870"
     CDS             160..711
                     /locus_tag="WHQ94_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2129101.1"
                     /translation="MSGININLKNGDSLLIRGPSGCGKTSLLRALAGLWPFGSSGKVS
                     RPPHQDILSLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYLVDKLDKTDD
                     WQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQKLPDSIIIS
                     IGHRSTLNAFHNMHLDVGNAACG"
     gene            816..1484
                     /gene="hda"
                     /locus_tag="WHQ94_001871"
     CDS             816..1484
                     /gene="hda"
                     /locus_tag="WHQ94_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ2129102.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            1481..2149
                     /locus_tag="WHQ94_001872"
     CDS             1481..2149
                     /locus_tag="WHQ94_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2129103.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT          918 a          890 c          644 g          763 t
ORIGIN      
        1 gtgaaaaagc tggcgtaact tttccggata caaaaaaacg ccgaaatcca cgtcttccga
       61 agatttcagc gtttttttac tctatttgtt ccttgtgcaa aaactaacag tcccccacag
      121 gaaaacgtcg ctctgttctg ccacaacggc gaaatcctgt tgagcggcat caacataaac
      181 ctcaaaaacg gcgattccct gctgatacgc ggcccgagcg gttgcggcaa aacctcgctg
      241 ttgcgcgcgc tggcggggct ttggccgttc ggcagcagcg gcaaagtcag ccgtccgccg
      301 catcaagaca tcctctccct gccgcaacgc ccgtacacgg cacagggcag cctgcgcgac
      361 gcggtttgtt accccgacat tgacaagcag catcccgagt tagccgaagc catgaacacc
      421 tgccgcttgg gttatctggt tgataaatta gataaaaccg acgactggca acacaaactc
      481 tccccgggcg aactgcaacg cgtcgccttc gtccgcgccc tgctttccaa gcccaaaatc
      541 gtcctgctcg acgaagccac cgccgctttg gacgaaccga ccgaggccct gctctaccgc
      601 gcactgaaac aaaaactgcc cgacagcatc atcatcagca tcggccaccg cagcacgctc
      661 aacgcgttcc acaatatgca cctcgatgtc ggcaacgctg cctgcggctg aacgatgccg
      721 tctgaagccg ttttcagacg gcatcgttca aattggaaag gcgcgcgaaa acaacgtaca
      781 ataaacccgt ttaccacccc tcggaatgcc gaaccgtgaa ccagcttatt ttcgactttg
      841 ccgcacacga ctatccgagt ttcgacaaat tcctcggcac ggaaaacgcg gaactggtct
      901 atgtcctccg acacaaacac ggacagttta tctatgtttg gggcgaagaa ggcgcgggca
      961 aaagccatct tttgcaggcg tgggtcgcac aggcgctcga agccggcaaa aacgccgcct
     1021 atatcgatgc cgcctccatg ccgctgaccg atgccgcgtt cgaggcggaa tacctcgccg
     1081 tcgatcaagt cgaaaaactg ggcaacgaag aacaagccct gcttttttcc atcttcaacc
     1141 gcttccgcaa cagcggcaaa ggctttttgc tgctcggttc ggaatacacg ccccagcagc
     1201 ttgtcatccg cgaagatttg cggacgcgta tggcgtactg cctcgtttac gaagtcaaac
     1261 ccttaaccga ccaagaaaaa atcgacgcgc tcgccagtat ggcggcggca cgccaagtaa
     1321 ccgtcgattc cgaaattttc gaatacctgc tcaaacactg gcggcgcgat atggacagcc
     1381 tgatgatgat gctcgacacg ctggacaact acgccgtcac aatgggcaaa cgcatcaccc
     1441 tgccgctttt gcgccagctt ttgaaacaac aggaaaccca atgaaaaacc tcgccatctt
     1501 cgacctcgac aacaccctca tcaacaccga ttccgaccac gcctggccgc aatacctcat
     1561 caaaaaaggc ttggttgacg ctgccgaaac cgaagcgcaa aacgaaaaat tctaccgcga
     1621 ctaccaaaac ggttgcctcg acatcgacgc attcctcaaa ttccaccttg ccccgctcgc
     1681 tcgttacagc aaagaagagc tggcagagtt tcaccgcgaa tttatggcgg aatacatcat
     1741 cccccacatc tcgcctatgc agcgtatgct ggtgcagagc caccaaatgg caggcgacga
     1801 aaccctcgtc atctcatcga ccaacgagtt catcatcacc cccgtctgcc gccttttcgg
     1861 cattaccaac atcatcggca cacaactcga atccggttcc gacggccgct acaccggcaa
     1921 ttacatcggc acgcccagcc tcaaagaagg taaaatcacc cgcctgaacc aatggctcgc
     1981 cgaacgcggc gaaacgctcg aaagctacgg caaaacctat ttttacagcg actccaaaaa
     2041 cgacctgccg ctgctgcgcc tcgtcgacga acccgtcgcc gtcaaccccg atgccgagct
     2101 ggaaaaagaa gccaaagaaa aaggctggcc ggttttgaat ttcaaatgac gcaatgccgt
     2161 ctgaagcccg attcggcgtt cagacggcat tgaggctgaa atccaaaaaa ctcaaagcca
     2221 caggaattta tccggaaaaa caaaaaacac aggaattcat cgggaaaaac aacaatcttt
     2281 ccaccgtcat tcccacgaaa gtgggaatct tgatccgtcc gcacggaaac ttatatttcg
     2341 tcatttgcac ccgacataat tcaatacttt attttgcaat aaattaacgg atactgacaa
     2401 attagattgg attaattacc ctattatcca tattgggtta tcgggttgct aattgagcag
     2461 agcgtagcaa accataaagt gagtgtttcc acccactctg catttatagt ggattaacaa
     2521 aaatcaggac aaggcgacaa agccgcagac agtacagata gtacggcaag gcgaggcaac
     2581 gctgtactgg tttaaattta atccactata ctatgaagtg gttattaaat atgataatga
     2641 gacctattaa atttattatg gtaaatacgt tattatttat tgttatatgt agttcatttt
     2701 ttgatctgct cgttcaatta tgtacaattt tatttcatag ccaaaaaata tactttatta
     2761 cattattttt attatttatt tttaattttg ttacaaaatc tatctatatg gcaattattt
     2821 atcctatttt atattttttt acgataaaaa aatattatcc ttactctagg aaagtgataa
     2881 ttctattatt attagcatta tctatatatt ttagttttat ggacttttac tttttttcca
     2941 tatattcaga taaccttagc tatgaaacgg agcctttaca tttatacatc cctattatta
     3001 ttaatttttt ctcactttta gtttctaatt ttattttatc ttttatcaac aagtaaatgt
     3061 atatacaaaa tacaatccaa aagatcctaa gcaaaccacc aatcccacac cacccttacc
     3121 ggttcaaatc cgcacaaaaa cccacatccc gtcattccca cgaaagtggg aatctaggac
     3181 gtatagtgga ttaacaaaaa tcaggacaag gcgac
//
LOCUS       DAWGJV010000173         3212 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWGJV010000173 DAWGJV010000000
VERSION     DAWGJV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.173"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            89..1042
                     /locus_tag="WHQ94_001873"
     CDS             89..1042
                     /locus_tag="WHQ94_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ2129104.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1153..1476)
                     /locus_tag="WHQ94_001874"
     CDS             complement(1153..1476)
                     /locus_tag="WHQ94_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129105.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1649..3010)
                     /locus_tag="WHQ94_001875"
     CDS             complement(1649..3010)
                     /locus_tag="WHQ94_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ2129106.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          756 a          939 c          777 g          740 t
ORIGIN      
        1 tgagctaagg cgaggcaacg ctgtactggt ttaaatctaa tccactatag attaaaatca
       61 cacaatatcc aactattcgg accttcatat gacacatacc gtccacctgc atttagaaga
      121 aaccgacaac ttggcgttgc agcgtctgtg cggttctttt gacaacaacc ttgatttact
      181 tgctaaagca ctcgatatcc acatcagccg ccgttttgaa catttcactt tcaacggcgc
      241 atttgcacac gccggcaaac gcgcactgct caaactcttg gaaacggcgc agacgcgcga
      301 cctaaacgac ggcgacatca ggcttgccgc cgtcgaagcc caaaccgaag atgccggtca
      361 tcaagaaaaa aaccatgacc acgcctatta tttccgcacc aagcgcggca gcatcggcgg
      421 cagaacgcca cggcagaacg gctatatccg cgccctactc aaccacgaca tcgtattcgg
      481 tctcgggccg gcaggtacgg gcaaaaccta tctcgccgtc gccgccgccg tcgatgcgat
      541 ggaaaaacac caagtcgaac gcatcatttt agtgcggcct gccgtcgaag ccggcgagaa
      601 actgggtttt ctgcccggtg acctgaccca gaaagtcgat ccctaccttc gtccgcttta
      661 tgatgccctc tatgacctga tgggctttga ccgtgtaacc aagctgattg aaaaaggcct
      721 gattgaaatc gccccgctcg cctatatgcg cggcaggacg ctcaacggcg catacatcat
      781 cctcgacgaa gcgcaaaaca ccacgcccga acaaatgaaa atgttcctga cccgcatcgg
      841 cttcggcgcg aaagccgtca ttaccggcga caccagccaa atcgacctgc ccaaaaacat
      901 caaatcggga ttaaaagatg cgcgtgagaa actgcacaac gtggaagggc tgtatttcca
      961 cacctttacc ggcgaagacg ttgtccggca tcctctggtg caaaaaatcg tcgaagccta
     1021 cgaatcggca gaacacgact gacattccaa tgccgtctga aacccgatgc atggaaaatc
     1081 gggcagagaa aaacagatac ggaagccaag ctccgtatcc gtatccgttt ctttatccgt
     1141 ccaaccgata catcaatcga attttctgca tcggattgcc tcatcttcgc aaaacattgc
     1201 cgaagtttcg cggttgcacg cctgacgcac tttctgccgc gccacgcctt cattccgatc
     1261 gcttgcctca aacgtcttct gaaaaggcgt aacgctgcat acatacatgg tggaatttga
     1321 accgaaaagg tcgattttga cggtattgct gccgctttcc aatacggcga cacggtgttc
     1381 caactgcgcc agacgtgctt ccaaatcgcc gattctgtcg gcaaatgcag gcatggcggc
     1441 acacgcggtc aaaatgccca aaatacattt tttcatcaat ttttccttgt ttcttgaacc
     1501 ctgcccaccc ggacatcctt tttccgagag gcagggttag actttattta cggggatgaa
     1561 tccccttaca aatcaggata atacatagaa cggcacttta tgcttcatcc catgtgttga
     1621 aacttcattg ctccttgtct gttgaccctt attcccaatc cgttcccaca accttcggct
     1681 tgggtttgcg cctgcgacgg cggcgttttt tcgcctgtcc ttcgtttttt tcgtgtcggg
     1741 cggcggcctc gtttttggtc atctccgacc gctgttccgt cgatgccgtc tgaaacgccg
     1801 tccaccactc ggcaagggcg cggtccgcat tgccggtttc ggcgcgcaag agcaggaaat
     1861 cataggcggc acggaaacgc gcctgtgcaa acagtttgtg cggacgtgcg cctttgcgat
     1921 tttcaaactg cggctggaac atccaaattt cgcgcatcgt ggcggaaaag cgttgcggca
     1981 cgccccaacc gcgttcgacg gtttcgcgca tcgtattgat tgcatcggac agggcgggca
     2041 cgggtttcaa accctgttgc agattgcttt tccaatggcg ttccaactcg ggccacatca
     2101 gggccgccag tacgaaaccg accgaaaccg atttgtcggc acgcagccgc tcatcggtat
     2161 ttttcagggc aagcaccgtc atttttccgg cgatgccgtc tgaaacgcgc aaggcattga
     2221 gcagcggatg gatgtcgtcc ggtatgtcaa atccgttcaa acgtttcaga cactcgcgcg
     2281 cgtgccctga aaacagcagt ttcataattt cgtcgaacag ccgcgctacc ggttcgtgct
     2341 tcagacggca tatcgattcg gcaatcggtg cggcggtttc ttccgacaga tcaaagccca
     2401 atttgctcga caggcggatg gcgcgcaaaa tcctgacagg gtcttcctga tagcgttcgg
     2461 cggcatcacc aatcataacc agcctgcggg cggcaacatc ggcaatcccg ttgtggaaat
     2521 ccaaaatctc ttctttttca ggatcgtaat acaaggcatt gcaggtaaaa tcgcgccgca
     2581 tcgcatcttc ttcgatgctg ccgtaggtgt tgtccttcat gattctgccg tgcgcgttct
     2641 gatgaacctt cgcaccgccg cgaaacgtcg ttacttcgat aatctccgca ccgttcatca
     2701 catggacaat ctgaaaacgc ctgccgatga tgcggctgcg gcggaagagt ttgtgcacct
     2761 gttcgggcgt ggcatcggtt gcgacatcga aatccttggg ttcgatgccg agcagcaggt
     2821 ccctgaccgc gccgccgacc acataagcct gaaaccccgc ccctttcagg cggcgtatga
     2881 cgttttcggc ggcaaagctc aacatttcgg cacggatgtt gtgtctttcg gcaggaatga
     2941 ttgttttact ttccgctttt ttactgctcc gaccggaagg cagcatctta ttcaaccatt
     3001 ttttcagcat aaatattcta acccgaagag cggcatctcc acgggcaacc gtgttcagac
     3061 ggcatcaggc ggtacgattc cgcaagatgc cgtaaccata aacaaaaggc gtagaaacta
     3121 cgccgtccga atcacgccgc cctcgcggcg gcggcaacgc gccattataa cagattgccc
     3181 tttaaaaaaa tcagaccctg cttttgtggc gg
//
LOCUS       DAWGJV010000174         3197 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWGJV010000174 DAWGJV010000000
VERSION     DAWGJV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.174"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..229)
                     /locus_tag="WHQ94_001876"
     CDS             complement(<1..229)
                     /locus_tag="WHQ94_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129107.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAG"
     gene            complement(213..530)
                     /locus_tag="WHQ94_001877"
     CDS             complement(213..530)
                     /locus_tag="WHQ94_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ2129108.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(658..936)
                     /locus_tag="WHQ94_001878"
     CDS             complement(658..936)
                     /locus_tag="WHQ94_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129109.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(943..1227)
                     /locus_tag="WHQ94_001879"
     CDS             complement(943..1227)
                     /locus_tag="WHQ94_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129110.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAVPL
                     ALATQANATLPDTAKNALEAAKEDGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            complement(1235..1432)
                     /locus_tag="WHQ94_001880"
     CDS             complement(1235..1432)
                     /locus_tag="WHQ94_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129111.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(1437..1727)
                     /locus_tag="WHQ94_001881"
     CDS             complement(1437..1727)
                     /locus_tag="WHQ94_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129112.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1772..3113)
                     /locus_tag="WHQ94_001882"
                     /pseudo
     CDS             complement(1772..3113)
                     /locus_tag="WHQ94_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT          808 a          730 c          692 g          967 t
ORIGIN      
        1 cccccgccaa tacgccggcg cgggaaatgc tggcggttat tttggcgttg attcgggctt
       61 ttgcgcccgt agggatgtgt gttaaatcta ccgtttttat taaatcagat gaataagttt
      121 tactattttt aggtacaaac ttatgaattt tcgcaccttg tccggtatca actgaaacag
      181 tttcagatat ttttactgca tttgcattcg cttcaaacga atacatcatc aaaattgcaa
      241 ttatcgacaa tttcgcaaaa ttcaaatttg tatattttat gaccatcttt cagggattct
      301 ttaattacca tttctgaatt atcagaaaat gagattagcc aaatatcatg tttaattctt
      361 ctattccaga aaaaagagaa acaatcaata acattttcag acttattaat cttcgcaaat
      421 tcaacaaatt cagattgcgc tataaccgcc atcgattgcc caaaatactt gcttgacggc
      481 tgatatttat aaagtgccaa ctgcgcctga gtgataaacg gcttgttcat ggttctgcct
      541 ttcaatgatt gttttgaaag cctgattttg acaccataac ttcatgcgct caattcttaa
      601 acagaaccgc cccgattaat acgggtacgg aaacgccgag ataaaaataa aaatccatca
      661 tttcaaaacc tttttcagca gggaaacaaa gtaaacggac gcgaggatgc cgaatactat
      721 ccagcctgtt tcaagaccgc tttgcaggtt gtctttcgga ctgcattccg ccaataaaag
      781 ccttagcggc tgaccgtccg acatcttcca caggctgccg ttatattccg gcctgacaat
      841 ctgtccgttt tctttgattc ttggtactac caagctgaaa taaaggtttt cagcctggtg
      901 cttctcaaga catttatttc cgacttggta gtacatgccg tcttacttca tcactctctt
      961 aacgatggaa aagacaaaaa gcgcggcgaa aatgcccact acaatccaac cggcttccat
     1021 accgtcctct tttgcggctt ccaaagcgtt ttttgccgta tcgggcaacg ttgcatttgc
     1081 ctgtgtcgcc aaagccagcg ggacagctgt tacaacagcc agttttgcgc cgtatttacg
     1141 gcaggtgtta ataaatttca tgatattttc cttcaaaaag tgtttggcgg taatggatgg
     1201 agcgtttttc agacgaccgc cgaacatccg aaaatcagtc tttcaaaaat ccgaatacga
     1261 caaattcgta ttggttgccg atttcttcca aacctgcgtt aatcgcttct tcgaagtcgt
     1321 agaaataatc ggcattggtg attaatttgg tatgtccgat gtcgcccgtt tcaggagaga
     1381 gatacagaaa gtcccctgtt gatacggact ggacaacata gactttctgc attcaatcag
     1441 cctttcttca cgagttgaaa accgatgact ttcagttttt gggttttgcc cgtagtaacg
     1501 atttctacgt tcaggtttgc ttcgatcgga aattgggcgt ttcggaactg ctcgaaattg
     1561 gcagagccgc cgaaatcgta ttcagtagta gagctgccca atgcgttgcc ttgggagctg
     1621 tctaagggtg tggcgacaat caggcggcaa tagtcgaagc tcctgccttc gatttgtccg
     1681 ttgaattttt taacgccgac gatgtggcct tgaagttgga tgttcatttt ttggtttcct
     1741 tgtgtgatta aacgtctttc gggcagacac tttaagccca tgaaatcggt agtcttgcga
     1801 atttgtcgta aatgaagttg ttatagcttt cttcattgtt gacgtgtttt tgctgttcaa
     1861 gctgtttttc aagattctcg taatattcgt acatatagta agggtcattg tacggtttga
     1921 atgcgggctg ttcatgaatg gcttgagctt tcaaaaaggc gcagtcgtag gcttcgggag
     1981 ccaaagactt gggcagcttg tgatgactcg gctcaatcag ttcaaacagt ttggctttgt
     2041 ccaattcggg aaaaatgaat ttcagaccgt ttgccgcacg tccgaactgt tttttaccca
     2101 ttcaaggtaa cggtcggctg aaatgacctt atcttcctta accgcgtgta tgcgcgttgc
     2161 cttttggacg aatcgttcgc aaatcggata tgcgccgccg aaatattcgc ccggattctg
     2221 caaaacttca aaagggataa cgatgtcttt tgctttgaat tcaatttcaa agcgcgtcca
     2281 tgtgcttgtt ttatcgccca actgcttgcc tttttcatag acgcggacat atttggacga
     2341 ttcacgggag ccgataccat aggtcttgcc tttggtcatt ttggcttcat cgtcttcttc
     2401 ccaatctgac cccaaacatt cgccttttgg tttgacgtga tgacaggtaa acataccttt
     2461 atttcggtct tcacgggctt ggttcgggct gtattcgccg ttgaaaaagt cttttgcgat
     2521 gtcaacgcgt gtgatttttg ggcggattgc attagtcagg aatgcgaaaa gtcgtgattc
     2581 ccagccttct tttgcgacgc cgcaaccggt gccggtcagt tcgaaaagaa tggtattgtt
     2641 ttgtccgcca aaatggacgc gaccgtatag gacgtcttcc gaacccatca accaacagcg
     2701 ctcataaaaa cggccgcccg agcctttgga ctctttgtag ataccgaaac cgaaaacttc
     2761 ttcggcgagc atggacgcgg cgcgaataaa atcttcgtct tccaaaagac ttacacgaac
     2821 gccgtattta tcgaaaaagg gtttttcatg aaatgaaaag ctaatttgat caatgaaagc
     2881 cgaatctgat acaccgcgcc gaagaggaac gcctaacagg tttcctttgc cgtccgttat
     2941 gtacgtttcg taacattcga agacttcctg aaccctgcct gccgtttcgg tttctgtccc
     3001 cccctgttag ataagggggg aagatttgaa gcggttgccg gcttcctgcc gtccgccggt
     3061 gcgtccgtca tcacgccgac aaccgccttt gtcatccctt gcttatcttc catggtgaga
     3121 atcctcaaaa acgggcaaaa aaaagccctg ttacttgtag aaagtaaagg acgttaattt
     3181 ttgttaatcg tcccttc
//
LOCUS       DAWGJV010000175         3134 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWGJV010000175 DAWGJV010000000
VERSION     DAWGJV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.175"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..1104
                     /gene="mafB"
                     /locus_tag="WHQ94_001883"
     CDS             <1..1104
                     /gene="mafB"
                     /locus_tag="WHQ94_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ2129113.1"
                     /translation="DPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFS
                     GHGHEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKP
                     TGARDEYTYNVNGVARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHN
                     AKLDRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVK
                     NLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGA
                     QGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSD
                     GKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            1107..1424
                     /locus_tag="WHQ94_001884"
     CDS             1107..1424
                     /locus_tag="WHQ94_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129114.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            1559..2236
                     /locus_tag="WHQ94_001885"
     CDS             1559..2236
                     /locus_tag="WHQ94_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ2129115.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYR
                     ETVYIKVSDGIGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPPRKTPSTPIYGNDSEAYAAAK
                     ELGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            2242..2640
                     /locus_tag="WHQ94_001886"
     CDS             2242..2640
                     /locus_tag="WHQ94_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ2129116.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            2671..3048
                     /locus_tag="WHQ94_001887"
     CDS             2671..3048
                     /locus_tag="WHQ94_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ2129117.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLLDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT         1035 a          635 c          689 g          775 t
ORIGIN      
        1 gacccgcgcg gcagcgtctc cgaccgcacc ggtcaaatca acgtcatcca agactatacc
       61 caccagatgg gcaacctgct catccaacag gcaaacatca acggcacaat cggctaccac
      121 acccgctttt ccggacacgg acacgaagaa cacgccccct tcgacaacca cgccgccgac
      181 agcgcaagca aagaacaagg caacgttgac gaaggcttta ccgtgtaccg gctcaactgg
      241 gaaggacacg agcaccatcc cgccgatgcc tacgacggcc cgaagggcgg caattacccc
      301 aaacctacgg gtgcacgcga cgaatacacc tataacgtta acggcgtagc ccgcagcatc
      361 aaactcaatc cgaccgacac ccgcgacgtc agacaacgca tatccgacaa ttacagcaac
      421 ctcggcagca atttctccga ccgcgccgat gaagccaaca gaaaaatgtt cgagcacaat
      481 gccaagctcg accgctgggg caacagcatg gagtttatca acggcgtcgc cgccggcgcg
      541 ctcaacccct ttatcagcgc gggcgaagcc gttgaccagt ggatgcagga aaaccccaat
      601 gccgccgaaa ccgtcgaagc cctggtcaac gtcctgccgt ttgccaaagt caaaaacctg
      661 acaaaggcgg caaaaccggg gaaggctgcg gttagtgggg atttctcaga ctcctacaag
      721 cataacactg cttcaagatt atctcagtct gtagatggag aaatgtttca aacccgcaat
      781 gttgatttta aagcaaaatc tattgggact aaaattcatg atggagctca agggaaacat
      841 atttcaggac atagaaacta cattgaaggt aagagtactt taaatcaaaa cattaatcct
      901 caagaattgt tgaacggaat acattcaggt gcttatccag ttatttctaa aggagcaaga
      961 ggaaatcctg ttgttgattt tgggtatcct ataggcagcg atgggaaatc aggattaagt
     1021 accaattttg gtacgattca ttcaggtaaa aatggagttc acattgttcc ggctaaccct
     1081 aaaaccatta aaaaggtgca atagttatga atatattacc aagctggctg cgagtcggta
     1141 tgaatatagc aatgctgggc atgatacact cagatatcag gttaattacc gtagattacg
     1201 aggaaggaag aaggttttta aaaatcaaaa attatttatc aagagaagcc atcacagaag
     1261 accatgaaga tatggaatat ttgattacag agttatggtc tatgtgtgga gaatattttg
     1321 atgaagctga ctttgaatgt atttattcta atcattcttc tatggagtta aaccaaataa
     1381 atggtgcagt attcaggaga aaggaattaa tttcgcaagc gtaggttaaa aaaaccaaca
     1441 atcacaatgt cttctgaaac cgtgtttaat tttcagacgg catttccttc atttgaaata
     1501 ggatattgag aactgagttc ttcaaaaatc ctacacctgc tccttccacg gcagcacctt
     1561 ggtcaaaacg gcagacggct acaaagccat tgcccatatt caagccggag accgcgtcct
     1621 ttccaaggac gaggcaagcg gagaaacggg atacaaaccc gttaccgccc gatacggtaa
     1681 tccgtatcgg gaaaccgttt acattaaagt ttcagacggc atcggtaaaa tacagaccct
     1741 cgtttccaat aaaatccacc cgttttacag tcaaggaaaa tggatacagg caggtcgtct
     1801 gaaaaaaggc gacaccctgc tttccgaaag cggcgcaaaa cagacggttc aaaacattac
     1861 cttcaaacag cagccgctca aagcctacaa tctgaccgtt gccgactggc atacttactt
     1921 tgtcaaaggc gataaggcgg aaacggaagg ggtttgggtt cataatgatt gtccgccaag
     1981 aaaaacacca tctaccccca tatatgggaa tgattcagaa gcatatgcag cagcaaagga
     2041 attgggatat agaaaaataa aagaaagaac gagaaatgat gctgctattt ttaaaaaagg
     2101 aaagagttat atatcacgcg atgtagattc acataatgga ggggcttgga aagaagcatc
     2161 ttcacccaaa aatctgaaca gaaaagaaac aagaaacggt acatttgata aaaacctaaa
     2221 tcgtattgga gattaattat tatgtgcgag ttcaaggatt ttagaagaaa catcccttgt
     2281 tttgaagagt atgacgaaaa ttcatttatt ggcaaatggt atgatgacgg ggtgtgggat
     2341 gatgaagaat attggaaatt ggagaatgct ttaatcgagg ttagaaaaaa atatccttat
     2401 ccgatggata taccaaggga catcgtgatt ggaatcggta ccattattga ttttttaatg
     2461 gttcaaaatt ggaaactttt tgaaattaaa gcttctcctt ggttgcctaa aagtgtcaaa
     2521 attaatgagc gttatgaaag gttcagagta atgctccgtt atatttttac tgatctagat
     2581 gtagaggact ggaaattttt ttactttcca atacaacata gtaaaggtag attgagatga
     2641 aaagtttaat tatattggag atacattatt atgtgtgagt tcaaggatat tataagaaac
     2701 gttccttatt ttgaggggta tgacgaaaat tcatttattg gcaaatggta tgatgacggg
     2761 gtgtgggatg atgaagaata ttggaagttg gagaatgatt taatcgaggt tagaaaaaaa
     2821 tatccttatc cgatggatat accaagggat attgtgattg gaatcagtac cattattgat
     2881 tttttaatgg ttccaaattg ggagcttttc gaaattaaag cttccccttg gctacttgat
     2941 agcgtgggaa ttcatgaacg ttatgaaaga ttcacaacaa tgctccgtta tatttttacc
     3001 gagaaagaca tagtcaacgt gcgatttgat tattacaaca aaaaatagca aaccccggca
     3061 atcaaaatgc cgtctgaaac cgtgtttaat tttcaggcga ccttcccctt atttgaaaca
     3121 ggatattggg aact
//
LOCUS       DAWGJV010000176         3117 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWGJV010000176 DAWGJV010000000
VERSION     DAWGJV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.176"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..958
                     /locus_tag="WHQ94_001888"
     CDS             <1..958
                     /locus_tag="WHQ94_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ2129118.1"
                     /translation="EKYFAQSLAAVVNQTWCNLDILIVDDGSTDGTLAIAKDFQKRDS
                     RIKILAQAQNSGLIPSLNIGLDELAKSGGGEYIARTDADDIAAPDWIEKIVGEMEKDR
                     SIIAMGAWLEVLSEEKDGNRLARHHRHGKIWKKPTRHEDIADFFPFGNPIHNNTMIMR
                     RSVIDGGLRYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKYSVRQH
                     EIAQGIQKTARNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLPEEDFERARRFLYQ
                     CFKRTDTLPAGAWLDFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            1000..1827
                     /locus_tag="WHQ94_001889"
     CDS             1000..1827
                     /locus_tag="WHQ94_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ2129119.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAIRFFLQHFVMLQPEQIKPIDLMMFSDFFDKEGMPVYQVSPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            1839..1934
                     /locus_tag="WHQ94_001890"
     CDS             1839..1934
                     /locus_tag="WHQ94_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ2129120.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            1976..2818
                     /locus_tag="WHQ94_001891"
     CDS             1976..2818
                     /locus_tag="WHQ94_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ2129121.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          826 a          721 c          828 g          742 t
ORIGIN      
        1 agaaaaatat ttcgcccaat cattagccgc cgtcgtgaat caaacttggt gcaacttgga
       61 tattttgatt gtcgatgacg gctcgacaga cggtacgctt gccattgcca aggattttca
      121 aaagcgggac agccgtatca aaatccttgc acaagctcaa aattccggcc tgattccctc
      181 tttaaacatc gggctggacg aattggcaaa gtcggggggg ggggaatata ttgcacgcac
      241 cgatgccgac gatattgccg cccccgactg gattgagaaa atcgtgggcg agatggaaaa
      301 agaccgcagc atcatcgcga tgggcgcgtg gctggaagtg ttgtcggaag aaaaggacgg
      361 caaccggctg gcgcggcatc acaggcacgg caaaatttgg aaaaagccga cccggcacga
      421 agatattgcc gactttttcc ctttcggcaa ccccatacac aacaacacga tgattatgcg
      481 gcgcagcgtc attgacggcg gtttgcgtta caacaccgag cgggattggg cggaagatta
      541 ccaattttgg tacgatgtca gtaaattggg caggctggct tattatcccg aagccttggt
      601 caaataccgc cttcacgcca atcaggtttc atccaaatac agcgtccgcc aacacgaaat
      661 cgcgcagggc atccaaaaaa ccgccagaaa cgattttttg cagtctatgg gttttaaaac
      721 ccggttcgac agccttgaat accgccaaat aaaagcagta gcgtatgaat tgctggagaa
      781 acatttgccg gaagaagatt ttgaacgcgc ccgccggttt ttgtaccaat gcttcaaacg
      841 gacggacacg ctgcccgccg gcgcgtggct ggattttgcg gcagacggca ggatgcggcg
      901 gctgtttacc ttgaggcaat acttcggcat tttgcaccga ttgctgaaaa accgttgaaa
      961 aacgccgctt tatccaacag acaaaaaaca ggataaatta tgcaaaacca cgttatcagc
     1021 ttagcttccg ccgcagaacg cagggcgcac attgccgcaa ccttcggcgc gcgcggcatc
     1081 ccgtttcagt ttttcgacgc actgatgccg tctgaaaggc tggaacaggc aatggcggaa
     1141 ctcgtccccg gcttgtcggc gcacccctat ttgagcggag tggaaaaagc ctgctttatg
     1201 agccacgccg tattgtggaa acaggcattg gacgaaggtc tgccgtatat cgccgtattt
     1261 gaggacgacg ttttactcgg cgaaggcgcg gagaaattcc ttgccgaaga cgcttggcta
     1321 aaagaacgct ttgaccccga ttccgccttt atcgtccgct tggaaacgat gtttatgcac
     1381 gtcctgacct cgccctccgg tgtggcggac tactgcgggc gtgcctttcc gctgttggaa
     1441 agcgaacact gggggacggc gggctatatc atttccaaaa aagcgatacg gtttttcctg
     1501 caacatttcg taatgttgca gccggaacag ataaaaccta tcgacttaat gatgttcagt
     1561 gattttttcg ataaggaggg gatgcctgtt tatcaggtca gtccggcctt atgcgcccaa
     1621 gagctgcatt atgccaagtt tcacgaccaa aacagcgcat tgggcagcct gatcgaacac
     1681 gaccgcctcc tgaaccgcaa acagcaaagg cgcgattccc ccgccaacac attcaaacgc
     1741 cgcctgatcc gcgccttgac caaaatcagc agggaaaggg aaaaacgccg gcaaaggcgc
     1801 gaacagttca ttgtgccttt ccaataaaag gaggagagat ggacatcgta tttgcagcag
     1861 acggcaggat gcggcggctg tttaccttga ggcaatactt cggcattttg cgccgattgc
     1921 tgaaaaaccg ttgaaaaacg ccgctttatc caacagacaa aaaacaggat aaattatgca
     1981 aaaccacgtt atcagcttag cttccgccgc agaacgcagg gcgcacattg ccgatacctt
     2041 cggcaggcac gacattccgt ttcagttttt cgacgcactg atgccgtctg aagaactgaa
     2101 tcgggtgatg gcggaattgg tgccgggatt ggcaaaacag cacctgttga gcgaagtgga
     2161 aaaagcctgc tttatgagcc acgccgtatt gtggaaacag gcattggatg aaggtctgcc
     2221 ctatgttgcc gtatttgagg atgatgtcct gcttggcaaa gacgcagaaa agttccttgc
     2281 cgaagatact tggttggaag agcgttttga taaggattct gcctttatcg ttcgtttgga
     2341 aacgatgttt gcgaaagtta ttgtcagacc ggataaagtc ctgaattatg agaaccggtc
     2401 atttcctttg ctggagagcg aacattgggg gacggctggc tatatcattt cgcgtgaggc
     2461 gatgcggttt ttcttggaaa ggtttgccgt tttgccggca gagtggatta aagcggtaga
     2521 ttggatgatg tttacttatt tctttgataa ggaggggatg cctgtttatc aggttaatcc
     2581 cgccttatgt acccaagaat tgcattatgc caagttcctc agtaaaaaca gtatgttggg
     2641 tagcgatttg gaaaaagata gggaacaaga aagaagacac cgccgttcgt tgaaggtgat
     2701 gtttgacttg aagcgtgctt tgggtaaatt cggtagggaa aagaagaaaa gaatggagcg
     2761 tcaaaggcag gcggagcttg agaaagctta cggcaggcgg gtcatatcgt tcaaatagtt
     2821 tgtgtaaaat atagcggatt aaaattagaa atggatacac tgtcattccc gcgaaagtgg
     2881 gaatccagtc cgttgagttt cagtcatttc cgataaatca ccgcaacgtt aaatttctag
     2941 attcccgctt tcgcgggaat gacggcggag agggtttgtt gtttcggata aatccctgta
     3001 gtttttcgtt tctagattcc cgctttcgcg ggaatgacgg cggggggatt tctgtttttc
     3061 cgacaaattc ctaaaactta aaattccgtt attcccgcga aaacagaaaa ccaaaaa
//
LOCUS       DAWGJV010000177         3116 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWGJV010000177 DAWGJV010000000
VERSION     DAWGJV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.177"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(273..1787)
                     /gene="katA"
                     /locus_tag="WHQ94_001892"
     CDS             complement(273..1787)
                     /gene="katA"
                     /locus_tag="WHQ94_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ2129122.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2135..2986
                     /locus_tag="WHQ94_001893"
     CDS             2135..2986
                     /locus_tag="WHQ94_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ2129123.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT          686 a          812 c          879 g          739 t
ORIGIN      
        1 ctttcgcggg aatgacgcgg aaaagttttc gtgccggttt gaaccggtgg ggatggtgcg
       61 ggaatgacgg cggaaaagtg ggctgtgggg cgggaatgac gggatatttt gcgtttaata
      121 aaaaacgccc actgaaacgg cgggggcggg atgggaaaat gccgtctgaa gcggtcaaac
      181 gacgtttcag acggcatttt tatgcccggt tatttccggt agcggacggc gcgggacagg
      241 atttcttcaa tttccatcca cataatgccc ccttacagca aaccggcctg acccagtgcg
      301 gggtcggtcg cgcgggcggc ttgtgcgtct tcgacagtca gtccgagggc ttttgccacg
      361 ccttcgccgt atgccggatc gcaacggtag cagttgcgga tatggcggta tttgatgaag
      421 tcgggcgcgt cgcccattgc ggcggcggtg ttgccgaaca atgcctgttt ctgcgcgtcg
      481 ttcatcaggt tgaacagggc gcgcggttgg ctgaaatagt cgtcatcgtc ttggcggtag
      541 tcccagtgcg ccgcgtcgcc gttgattttc aaaggcggtt cggcgaagtc gggctgctgc
      601 tgccattggc cgaagctgtt gggttcgtag tgcggcaggc tgccgtagtt gccgtcggcg
      661 cgtccttgtc cgtcgcgctg gttgctgtga acagggcaac gcggacggtt gacagggatt
      721 tggcggaagt tcacgcccaa gcggtagcgt tgcgcgtcgg cgtaattgaa caaacgggct
      781 tgcaacattt tatctgggct ggcgccgaca ccgggaacga ggttgctcgg tgcgaaggcg
      841 gattgttcca catcggcgaa gaagttttcg ggattgcggt tcaactcgaa ttcgcccact
      901 tcaatcagcg gatagtcttt tttcggccaa actttggtca agtcaaacgg atgataaggt
      961 actttttccg cgtctgcttc aggcatgact tggatgtaca tcgtccattt cggaaactcg
     1021 ccgcgttcga tggcttcgta taagtcgcgc tgatggcttt cgcggtcgtc ggcgatgatt
     1081 ttggcggctt cttcgttggt caggttttta atgccttgtt gggtgcggaa atggaatttc
     1141 acccaaaaac gctcgcctgc ttcgttccag aagctgtagg tatgcgaacc gaagccgtgc
     1201 atatggcggt agccggcggg gatgccgcgg tcgctcatca cgatggtaac ttggtgcagt
     1261 gcttcgggca gcagcgtcca gaagtcccag ttgtttgtgg cagagcgcat attggtgcgc
     1321 gggtcgcgtt tgacggcttt gttcaggtcg gggaacttac gcgggtcgcg caggaagaac
     1381 acgggcgtgt tgttgccgac cacatcccag ttgccttctt cggtataaaa tttcaaggca
     1441 aaaccgcgga tgtcgcgttc tgcatcggct gcgccgcgtt cgcctgccac ggtggtgaaa
     1501 cgggcgaaca tctcggtttt tttgccgact tcgctgaaga ttttggcgcg ggtgtatttg
     1561 gtgatgtcgt gcgttacggt aaacgtaccg aacgcgcccg aacctttggc gtgcatacgg
     1621 cgttcgggga tgacttcgcg cacgaagtcg gcgagttttt cattcagcca caaatcctgc
     1681 gccagcagag ggccgcgagg accggcggtc aggctgtttt gattgtcggc aacaggcgcg
     1741 ccgttgttca tggtcagatg ggttacaggg catttggagg tagtcatcgc tcttgttcct
     1801 tttctcaggt tggtcaaatg ggggtaaacg gcttacagta cgatttggcg gaaagcgtat
     1861 tcgtaaccgg tttcttgatt gcaataaatt tcttgaatcg acattttatt tcccttttgt
     1921 aaaaactatg gatgcgacta tacgccaaga ttttcgctat taaaactatg aaatcgattt
     1981 aatattatta taagcaatcg gttcttgatt ttcgtttgtt ttttgttatc gaacggaatc
     2041 cgaacccgct cattaaaacc atttataatg caatgacgct ttgcggcatt ttttgcgccg
     2101 gcaggctgaa aataacaatt ttccccacat tatcatgacc ttactcggaa taaagctcaa
     2161 acagacccag cagctcaacc agcggctgca acaatctttg cgcgtattgc agatgtcggg
     2221 tatcgaactt gaacgcgagg tcgaaaactg gctgtcggac aaccccctgc tcgaacgcaa
     2281 agacacggat gaattttccg atgccgagtt cagccattac actgcacccg cgcgccaaat
     2341 cggcggagac gaaggcgaag atatgctgtc caacatcgcc ggcgagcagg atttcaagca
     2401 atacctgcac gcgcaagtat gcgaacaccc gctttccgac caagaatccg cctgtgtcca
     2461 catcctcatc gatttccttg acgagcaagg ttatctgacc gacagcatcg aagacatcct
     2521 cgaccatacg cccttagagt ggatgttgga tgaagcaatg ctgcaacacg cgctgaccgc
     2581 attgaaaaaa ttcgacccgg caggcgtggc cgccgccgat ttgaacgaat cgctgatact
     2641 gcagatagaa agattgggcg aatgtgctgc caaacccgcc gccctgcata tcgtccgaaa
     2701 cgccctcgac agcattgacg gcaaccgcag ccaaaccctc gcacgaataa aaaaacacct
     2761 gccccaaacc gacagcggca cactcgaagc cgcactcgac ctcattgctt cgctcaatcc
     2821 ctttcccgcc gccggttttg cctcgtccac acccacgccg tattctgacg aggcactcgc
     2881 caacctgctg gctttccgcg gcatggaggt ttctcgccgc accattgcca aatacagaga
     2941 atcccttgag attccggcag cacacaaacg caaaaccgca gaataattgc cgaataatct
     3001 tataaagaca acaaaccaaa agccggcatt tctgcgaaag cgggaatgcc gaatccgtcc
     3061 gcgcggaaac ctgcatcccg tcattcccgc gaaagaggga atctagaaac gcaaag
//
LOCUS       DAWGJV010000178         3101 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWGJV010000178 DAWGJV010000000
VERSION     DAWGJV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.178"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..211)
                     /locus_tag="WHQ94_001894"
     CDS             complement(<1..211)
                     /locus_tag="WHQ94_001894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129124.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
     gene            271..894
                     /locus_tag="WHQ94_001895"
     CDS             271..894
                     /locus_tag="WHQ94_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ2129125.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WHQ94_001896"
     CDS             1005..2387
                     /locus_tag="WHQ94_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2129126.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLIAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="WHQ94_001897"
     CDS             2391..2915
                     /locus_tag="WHQ94_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ2129127.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGCNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          620 a          838 c          940 g          703 t
ORIGIN      
        1 cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg ctgcataatc cggacgacgg
       61 ataaccggtg aaaacggaaa tgcccgggcg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtagaattga ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgcgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgagaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgagcgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgtcgg gctgattgtg tttgcagcgg gcagcttcct tgccgccgcc gccgatacgc
     1321 tgcccatgct gatcgccgca cgcgccatac agggtgcggg agcggttagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtctgacg ttttcggtca gcctcgtcgt tgcccctgtg attgccgacg
     1501 cggtgggcgt acgcggcttg tttctgctga ccggtatttt gaccgttgcc agtatcggcg
     1561 tggtggcgtg gatgactccc gacccggagg tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctc gatttcggca
     1681 ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccctcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctc aaacaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggtgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct g
//
LOCUS       DAWGJV010000179         3060 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWGJV010000179 DAWGJV010000000
VERSION     DAWGJV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.179"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            245..2467
                     /locus_tag="WHQ94_001898"
     CDS             245..2467
                     /locus_tag="WHQ94_001898"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ2129128.1"
                     /translation="MTKSTIIYTYTDEAPALATQSLLPIVQAFTRHADIDVKTSDISL
                     SGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQS
                     KGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNPH
                     SMGAWAKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALKA
                     GEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFFA
                     PVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVDS
                     DKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATIDF
                     CREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVVDAAGKVLMQHDV
                     EAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLAE
                     LDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGNT
                     KAQVLADTLDAATEKLLLNDKSPKRKTGELDNRGSHFYLTLYWAQELAAQDKDAELKA
                     AFAPLAKSLAENEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQALSTL
                     "
     gene            2610..2918
                     /locus_tag="WHQ94_001899"
     CDS             2610..2918
                     /locus_tag="WHQ94_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129129.1"
                     /translation="MKTVPTILISILLATALPASAHGIHKSKPLTMDELPPICQQYFK
                     RAETCYNKAGNKANFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRISNSNAV
                     "
     gene            2908..3060
                     /locus_tag="WHQ94_001900"
     CDS             2908..3060
                     /locus_tag="WHQ94_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129130.1"
                     /translation="MPSETMISDGILTSKRTSLIKPAFPASLTRTGLNEVTALTIMAS
                     SHSQKL"
BASE COUNT          871 a          913 c          674 g          602 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttctccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagtgcga acaaatccgc cgaaatatgc cataataggc
      181 ttagcacgtt tcttctcgcg atgtgcccga aaagaaaaac ttaacctaca tagagaataa
      241 tcccatgact aaatccacca ttatctatac ctacaccgac gaagctcctg cattggccac
      301 tcaatccctg ttgccaatcg tgcaggcgtt tacccgccat gccgatattg atgtcaagac
      361 cagcgacatc tctctctccg gccgtatttt ggcggcgttt ccagaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcatc cgtacctcaa ctgactgccg cgattaaaga
      541 attgcagtct aaaggctttg ccgttcccga ctatcctgcc gaccctcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgcc gcgcccccaa agcagtgaaa aactttgcca aaaaaaatcc
      721 gcacagtatg ggcgcgtggg cgaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttc cataacgaac aatccgttac cgtacccgaa gcgacttccg tatccatcgt
      841 gttcaccgac aaacaaggca acaaaaaaga gctgcgcgcg cctgttgccc tgaaagccgg
      901 cgaaatcatc gacgcaaccg tgatgagcaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtgaaagac gcgaaggcaa aaggcgtatt gttctcactg catatgaaag ccacgatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtgttcttcg cgcctgtatt
     1081 tgaaaaattc ggcggcaaac tggctgccgc aggtgtcaac gtcaacaacg gtttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc gccgccgtcg aagctgaaat
     1201 cgccgccgtt tacgccgcca accctgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgacgcttct atgcctgcga tgattcgtaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcacaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggtcaggttc aagtagttga
     1561 tgcggcagga aaagtcctga tgcagcacga cgttgaagca ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactc gccgtcaacc gcgcccgcct
     1681 gagcaatacg cccgccgtgt tctggctcga cgaaaaccgt ccgcatgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaac ggcctcgaca tccgcgtcct
     1801 cgctcccgaa gaagccgcca agttcagcct gggccgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccga ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca cgtccaacaa ttccttgaag aaaaccactt
     2041 gcgttgggac tctttgggcg aattcctcgc gctcgccgta tcgtttgaac atttggcaca
     2101 aaaaaccggc aacaccaaag cccaagtcct cgccgacact ttggatgccg ccaccgaaaa
     2161 actgctgcta aacgacaaat cgcccaaacg caaaaccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 attgaaagcc gcatttgcgc cgttagccaa atcgttggcg gaaaacgaag ccaaaatcgt
     2341 tgaagaactc tctgccgtac aaggcaaagc ggtcgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gcggcacaag cgatgcgccc aagcgcaacc tttaatcagg cactaagcac
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 ttcctatctg cctgattatg attaaacgcc gcacccattg gatataaatc atataatctt
     2581 tagtttttac cctaggaaaa gacaataata tgaaaactgt tccaaccatc ctgattagca
     2641 tccttttggc aaccgccctt cctgcatccg cacacggtat acataagagc aagcccctga
     2701 ctatggacga gctgccgccg atttgccaac aatatttcaa acgcgccgaa acctgttaca
     2761 acaaagccgg caacaaggca aattttgcac gcaacaacac taaattccta ttccaagccc
     2821 tgcctgctgc cgatttggga cagcgtaagc aaatgtgcca aatcgctatg gattcgtttg
     2881 cagaaaaaac ccgcatcagc aatagcaatg ccgtctgaaa caatgatttc agacggcatt
     2941 ctcacatcaa aacgcactag cttgataaaa cccgccttcc ccgcatccct gacccgaaca
     3001 ggattaaacg aagtaacggc tttaaccatt atggcaagtt cccattctca aaaattatag
//
LOCUS       DAWGJV010000180         3055 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWGJV010000180 DAWGJV010000000
VERSION     DAWGJV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.180"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..1058
                     /locus_tag="WHQ94_001901"
     CDS             <1..1058
                     /locus_tag="WHQ94_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ2129131.1"
                     /translation="APGGYIVDIPKGNLKTEIEKLSKQPEYAYLKQLQVAKNVNWNQV
                     QLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFASLAS
                     QASVSLINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETPWVNN
                     LNVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHK
                     VAHAVAGCAAAAANKGKCQDGAIGAAVGEILGEALLDGRDPGSLNVKDRAKIIAKAKL
                     AAGTVAALSKGDVNAAANAAAVAVESNALSKERMDKLTKCLSGKTCSTTMEKVNAIKK
                     DEQFSKVIDTEIQKSLF"
     gene            1066..1761
                     /locus_tag="WHQ94_001902"
                     /pseudo
     CDS             1066..1761
                     /locus_tag="WHQ94_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            1749..2192
                     /locus_tag="WHQ94_001903"
     CDS             1749..2192
                     /locus_tag="WHQ94_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129132.1"
                     /translation="MQAMNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSF
                     LGVIDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSL
                     FNSSERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(2260..2528)
                     /locus_tag="WHQ94_001904"
                     /pseudo
     CDS             complement(2260..2528)
                     /locus_tag="WHQ94_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2527..2700
                     /locus_tag="WHQ94_001905"
     CDS             2527..2700
                     /locus_tag="WHQ94_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129133.1"
                     /translation="MIKQVANNLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LSVKVPSKTNIRR"
     gene            2703..>3055
                     /locus_tag="WHQ94_001906"
     CDS             2703..>3055
                     /locus_tag="WHQ94_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ2129134.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSP"
BASE COUNT          899 a          570 c          732 g          854 t
ORIGIN      
        1 ccgctcccgg cggctatatc gtcgacattc cgaaaggcaa tctgaaaacc gaaatcgaaa
       61 agctgtccaa acagcccgag tatgcctatc tgaaacagct ccaagtagcg aaaaacgtca
      121 actggaacca ggtacagctg gcgtacgaca aatgggacta taagcaggaa ggcttaacca
      181 gagccggtgc agcgattatc gcgctggctg ttaccgtggt tactgcgggc gcgggagtcg
      241 gagccgcact aggcttaaac ggcgcagccg cagcagcggc cgatgccgcc tttgcctcac
      301 tcgcttctca ggcttccgta tcgctcatca acaataaagg cgatgtcggc aaaaccctga
      361 aggaactggg cagaagccgc acggtaaaaa atctggttgt agcggcggca acggcaggcg
      421 tatccaacaa actcggtgcc tcttcccttg ccacttggag cgaaacccct tgggtaaaca
      481 acctcaacgt taacctggcc aatgcgggca gtgccgcgct gatcaacacc gctgttaacg
      541 gcggcagcct gaaagacaat ctggaggcaa atatcctggc ggcattggtg aataccgcgc
      601 atggggaggc tgcgagtaag attaagcaat tggatcagca ctatatcgcc cacaaagttg
      661 cccatgccgt agcaggctgt gcagctgcgg cggcgaataa gggcaagtgt caggacggcg
      721 cgataggagc ggcggtcggt gaaatcctcg gcgaagccct actggacggc agagaccctg
      781 gcagcctgaa tgtgaaggac agggcaaaaa tcattgctaa ggcgaagctg gcagcaggga
      841 cggttgcggc gttgagcaag ggggatgtga atgctgcggc gaatgcggct gctgtggcgg
      901 tagagagtaa tgcgcttagc aaggaaagaa tggataaatt gacaaaatgc ctttccggta
      961 aaacttgttc tactacgatg gaaaaagtaa atgccatcaa aaaggatgaa caatttagca
     1021 aagtaattga tacggaaatt caaaaaagtc tgttctagga acccattggg cgatggttgc
     1081 agaaacggca ttaatatgtc tattaaatat attgccatgc ctgctgcgtg gaagtatatg
     1141 cctacggatg tatcacgggt tgccaaagaa gtttttggct atttatataa ctcacaaggg
     1201 gcatctacaa gatttgacaa gtatttcaac accattgaca atcgtgcaga tttctttgct
     1261 gccagcaatc tttatgagca aaatttgggt tcaaaagcac gatggtttgg tggagctgat
     1321 tttgtatcgc gtgctgctat aactgggtta ggggcagacg gagaagcttc ttatataact
     1381 tttgcggcag gtaaagttgt tggtaatcct ccaatttatg aatggagggc tgcgtcaggc
     1441 aatgcactga tagtaaatgg attttataat tttagagact tgttcaataa aaaaactaat
     1501 cctagggagt gggatattca acagttgaaa agcgagcaaa agttattaca gcctattcaa
     1561 ccaaaaatat ttgagtaatg agaaggatta tttgtcattg attaaagggg taacatcaaa
     1621 taagatattt tcaataattc caaatccttt agatgagagg aaaaaataga ggatggaatt
     1681 aatatgttag attacaaatc tagaattaaa tatggttgta agcttatggg atattctgag
     1741 aaacagggat gcaagccatg aataaaaatt atttatttta tcttattcaa aatattaaat
     1801 ctattttctt aataggtgga gtattttttg gccttttaat tttttcaggt ttatggggta
     1861 atgattttaa actatctttt ttaggggtaa tagattttat atttgggatg gtagggggtg
     1921 ggtttatagt tatgttatgg tttctaattc cagtgatatt atcaacttta ttcagatggg
     1981 tatttcaaaa tcaacttgct gtatctgtca tattaacctt cattgttttt tatacatatg
     2041 ggtacttttc tttatttaat tcatctgaaa gagtcggtag tttttggtat ttggtaatcc
     2101 caaccataat gtttattgga ctgtctgaat ggtttatcta taaatctagc gtgcatagtt
     2161 taggtgaaaa tgtaactcca cgttccaatt gaagaaaaag attgtctgaa aactaaattt
     2221 aatttcagat gaccttagat tcggatttca agtacaacac tagggtatcg atggttggaa
     2281 caaattcaag aataaaacac ttggcgtttc gtagccaagt gtttttctcg gtcggtggtt
     2341 caactcatct tgaaccctac gtatctcccg atcgctgatg tttcggaaaa cggtttgttt
     2401 ggggaatatt gtcggatgag tccattggtg ttttcattca gccctttctc ccaagaatgg
     2461 taagggcggt aaaaataggt ttccgccttc aatgttttgg ccatttggat gtgtcggtag
     2521 aattctttga ttaaacaagt tgcaaataac ttacctgatg gtagtcctgc taaagctgct
     2581 gtcttcaaag cggagcggga aggcaaatta aaaacggcaa tagcaggcgt tgatcgtcaa
     2641 acaggtaagg ccgttattct ttctgtcaaa gttccttcta aaaccaatat aaggagataa
     2701 caatggggca caatatgatg accacccaaa aatggtatga gcatattact aatgtaatca
     2761 taggcaatac tgctaatttc aatagcggtt gccctgaagc tatagattat gtagatgaaa
     2821 gaaaaggcgt tccacttgca gcaatgagac atattttaat gtacactgaa gctgcggctt
     2881 cccatgccta tctatttgaa catgatctta ataaattcaa gcaatatgct tatgttgcag
     2941 gaaagttggg tattttgcgg agtgtaaatt ctacagaccc tgaacccttc ttctttccct
     3001 gtgacatgct caacattcaa gatccgatgt ttctgatgct gatgagcgac agccc
//
LOCUS       DAWGJV010000181         2995 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWGJV010000181 DAWGJV010000000
VERSION     DAWGJV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.181"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(416..952)
                     /locus_tag="WHQ94_001907"
     CDS             complement(416..952)
                     /locus_tag="WHQ94_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ2129135.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="WHQ94_001908"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="WHQ94_001908"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ2129136.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="WHQ94_001909"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="WHQ94_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ2129137.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2077..2922
                     /locus_tag="WHQ94_001910"
     CDS             2077..2922
                     /locus_tag="WHQ94_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ2129138.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
BASE COUNT          739 a          914 c          636 g          706 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataccgccac tgccgtacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgctcccgga tgacggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacaa
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagtg tttcgcatac ctcggcataa tgtaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacgccgat aagcgtatcc gccttgatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accacaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctacg
     1501 gatcgggcaa tcctggggcg accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgctgccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cccgcaactg ccttggtctg cgcgttggtt tggcttgtta
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccac aaaagtaata tcgtcaagct gctcgaaggc agagaaagca
     1981 aaatcggcgg cagccgctga tttcccatcc atccctgttt atgccgtctg aaccccgctt
     2041 cagacggcat ccgaatatcc cgccgtttta ccgactatgc tcagttacag acacgcattc
     2101 cacgccggca accacgccga tatgctcaaa cacttcacct tgtttttagt gctgcaatat
     2161 ttcaaccgca aagacaagcc ctactggtac atcgacacgc acggcggcgc gggtgtgtac
     2221 aatctcgaag gcagcgaggc acaaaaagtc ggcgaatacc ggcaaggcat cgccctactt
     2281 cgacaagcaa aaaacctgcc aaccgagtta aacggcttta tcgcgcacat acaaaaaatc
     2341 ctgccctcgc ccgaacttta ctgcggttct ccgtggctgg cgcaatcgct gacccgcgtc
     2401 ggcgacaaat tacgcctgtt tgagctgcac cccaccgact ttgtccatct gcaaaacaat
     2461 atgggcgaag caggattggg caaacgcgga caagtgttgc gcgaagacgg ttacaaaggg
     2521 ctgatttccc tgctgccgcc cccgccgcgc cgcgccaccg tcctcatcga cccgccctat
     2581 gaggaaaaac aggactaccg gcgtgtaacg gaaacgctga aagccgcttt aaaacggttt
     2641 gagtccggct gttatctcat ttggtatccc tgcctcagcc gcgaagaaag ccgcaagctg
     2701 cccgaagaat tgaaaaaact cctgcccgac aactaccttc acgccgaact tcacgtccac
     2761 gcgccgaaag ccgacggttt cggtatgcac ggcagcggaa tgttcgtcat caatccgccc
     2821 tatctgttgg cggaacaatt ggcggcaaac ctccccgccc tgacgcggct gctggcgcaa
     2881 gacgaaggcg cgcgctacct tttggacagc aaaatccgct gaccccgatg ccgtctgaaa
     2941 cccttcagac ggcatctccc atcaaacaaa accggacgat ataccggcag ccggc
//
LOCUS       DAWGJV010000182         2977 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWGJV010000182 DAWGJV010000000
VERSION     DAWGJV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.182"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            112..2877
                     /locus_tag="WHQ94_001911"
     CDS             112..2877
                     /locus_tag="WHQ94_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2129139.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          846 a          748 c          726 g          657 t
ORIGIN      
        1 caattgaaat cttcagacgg tatatcgaat ttacactttt taatgtttat gccgcctaaa
       61 aaaatgctaa tatatttctt aattgtctga ctgtttattg ttgaggaaaa tatgagatct
      121 tctttccggt tgaagccgat ttgtttttat cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcata
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctggaacgg cgcaagcaac gatattttgt acaagacggc
      901 cagttgaaat tcaattccga cagcggaaaa tgggagcggg acttgcaaag acaacagtgg
      961 aaatacccgt ggtataaaaa atacgaaaac ccccaagaac tgcaaaaata catcgaagag
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggttta tctttaaact catatgccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aagattttga aacctacaac aacgcgaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg cgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtccg
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccatcgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac ggctcggatg acgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata cagggaacat tgtaaccaga gctgcggaat ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggattgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg aatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca ccgcaatttc aaagacaaag tacacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagc tcgttcgacc cgaaagacaa ggacgaagac
     2761 gtaacgtgta atgctgatgg cttgtgcaac ggcaaatacg gcggcacaag caaaagcgta
     2821 ttgaccaatt ttgcacgcgg acgcaccttt ttgataacga tgagctacaa gttttaaagg
     2881 cagcccgcgt tttgtggaaa accgcaatgc cgtctgaaag cccttcagac ggcatttgtt
     2941 tccccaaacg catcatcctg ccgcaagcct atgccaa
//
LOCUS       DAWGJV010000183         2967 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWGJV010000183 DAWGJV010000000
VERSION     DAWGJV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.183"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            121..1665
                     /gene="purF"
                     /locus_tag="WHQ94_001912"
     CDS             121..1665
                     /gene="purF"
                     /locus_tag="WHQ94_001912"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ2129140.1"
                     /translation="MCGVLGLVSHEPVNQFLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHLSRMEHSISIS
                     DAGDEE"
     gene            1765..2256
                     /gene="greB"
                     /locus_tag="WHQ94_001913"
     CDS             1765..2256
                     /gene="greB"
                     /locus_tag="WHQ94_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ2129141.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2315..2941
                     /locus_tag="WHQ94_001914"
     CDS             2315..2941
                     /locus_tag="WHQ94_001914"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ2129142.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          713 a          905 c          773 g          576 t
ORIGIN      
        1 tattctccat aaacccacct atctgtcctt cgctcaaata ttgtcaacaa ttcaggcaaa
       61 aaaccaccgt ttactttata attccgtttt acccttaaac ctcgaatcgg agaagacgat
      121 atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagtttct gtacgacggc
      181 ttgcagatgt tgcagcacag ggggcaggat gcggcgggca ttgcaacggc ggaaggcggt
      241 accttccata tgcacaaagg caaaggcatg gtgcgcgaag tgttccgcac acgcaatatg
      301 cgcgatttga ccggcaacgc cggtatcgcc cacgtccgtt atcctactgc gggcaacgca
      361 ggcagcagcg cggaggcgca gcctttctac gtcagctcgc cgttcggcat cgttttggcg
      421 cacaacggca acctcaccaa cactgccgaa ctgtatgaaa acgtgtgtaa caaacacctg
      481 cgccacgtca acaccagctc cgattccgaa gtcttgctca acgtattcgc gcacgaatta
      541 cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa tattttcaac
      601 gccgttgccc aagtccaccg cctggtgcgc ggcgcatacg gtgtgattgc catgattgcg
      661 ggctacggta tgctcgcctt ccgcgaccct tacggcatcc gcccgctggt attgggttcg
      721 caaaccgaca gcgaaggcag aaaatcctat gccgtcgcct ccgaatccgt cgccttcaac
      781 gcgcttacct acgatttgga acgcgacatc cggccgggcg aagcggtatt tgtcggcttt
      841 gacggcacaa tgattgcccg ccaatgcagc gaccgtgcca aactcagccc ctgccttttt
      901 gaatatgttt attttgcccg ccccgactct gtgattgacg gcgtatccgt ttaccaatcg
      961 cgcttggata tgggcgtgtc gctggcggaa aaaatcaaac gcgagctgcc cgtggacggc
     1021 atcgatgtcg tgatgcccat tcccgacacc agccgcccca gcgcgatgga gcttgccgtc
     1081 catctcgaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg ccgcaccttt
     1141 attatgcccg gacaggcgac gcgcaaaaaa tccgtgcgcc aaaaactcag cccgatggaa
     1201 accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg cgggacgacc
     1261 agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta tatcgcctcc
     1321 gccgcgcccg aagtgcgcta tcccaatgtg tacggcattg atatgcccac gcgcgaagag
     1381 ttgattgcca acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc ggacggcatc
     1441 gtatttcaag acttgggcga tttggaagcc gtcgtcaaag cactcaatcc gaaaatcgaa
     1501 tccttcgatt cgtcctgttt caacggcatt tatcagaccg gcgacatcga tgatgcctac
     1561 ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca cctgagcagg
     1621 atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt cccaaaccgc
     1681 gatgccgtct gaaatccgaa cgccgggttt tcagacggca tttcccatat cggcatcaca
     1741 tttcccgttt catccgcaca caatatgagt accgaaacca aaaactacat tacgcccgcc
     1801 ggatggcagg cattgaaaga cgagctttat caactggtca acaaagaacg ccccgaaatc
     1861 gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg cgactatctt
     1921 tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac caagcgtttg
     1981 gagtccgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt atttttcggc
     2041 gcgaccgtcg gcctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat cgtcggcatc
     2101 gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc gcgttgcctg
     2161 attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaagggcg tgaggaaatt
     2221 gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc tgaaacggtg
     2281 caagcgcgcc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca ccaaaatctg
     2341 cggcatcacc acaccggaag acgcatcggc cgccgtagcg gcaggtgcag atgccgtcgg
     2401 gctggtcttt ttccaaggca gcagccgggc cgtcgatatt gcccgcgcca aaaaaatcac
     2461 cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc gtcaacgaaa gcgcgcaaaa
     2521 catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg gcgacgaaga
     2581 cgacacattc tgccgtcagt tccaccgccc ctatatcaag gccattcgtg ttcagacgac
     2641 atcagacatc cgaaacgccg ccgaccgctt ccccgacgct caggcactgc ttttcgatgc
     2701 ctaccatcct tcggaatacg gcggcaccgg acaccgcttc gactggacgc tgctggcgga
     2761 atattcgggc aaaccgtggg tacttgccgg cgggctgacc cctgaaaacg tcggcgaagc
     2821 cgtccgcatc accggagcgg aagcggtcga tgtatccggc ggcgtggaag cgtctaaagg
     2881 caaaaaagac ccagccaaag tcgccgcctt tatcgcaacc gccaaccgcc tatcccgtta
     2941 aagcaacaaa aattgccgcc ggaatga
//
LOCUS       DAWGJV010000184         2936 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWGJV010000184 DAWGJV010000000
VERSION     DAWGJV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.184"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..242
                     /locus_tag="WHQ94_001915"
     CDS             <1..242
                     /locus_tag="WHQ94_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ2129143.1"
                     /translation="FYYIKKGENPAIITHWNNRVNQAEEDNYSTGVGSCINGFTVQYY
                     PFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            259..534
                     /locus_tag="WHQ94_001916"
     CDS             259..534
                     /locus_tag="WHQ94_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129144.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(621..1754)
                     /locus_tag="WHQ94_001917"
     CDS             complement(621..1754)
                     /locus_tag="WHQ94_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129145.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLNYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRNSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1928..2377)
                     /locus_tag="WHQ94_001918"
     CDS             complement(1928..2377)
                     /locus_tag="WHQ94_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129146.1"
                     /translation="MENRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWERSGKPLDWEKWQRQYRS"
     gene            complement(2544..2867)
                     /locus_tag="WHQ94_001919"
     CDS             complement(2544..2867)
                     /locus_tag="WHQ94_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129147.1"
                     /translation="MNILASDRNYELNIEIFIDNIIHFQITDESYKVKFSEYLFENKE
                     ENNSDGNPTANYFFEIIDDSYMDWLKEESFDFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT          835 a          671 c          537 g          893 t
ORIGIN      
        1 aattttacta tattaaaaaa ggagaaaatc cggcgattat tactcattgg aataatcgag
       61 taaaccaggc tgaagaagat aattatagca ctggcgtagg ttcctgtatt aacggtttca
      121 cggtacagta ttacccgttt attcgggaaa agcagcagct cacacagcag gagttggtag
      181 gttatcacca acaagtagag caattggtac agagttttgt aaacaatcca agtaaaaaat
      241 aatttaaagg atcttattat gaatgagggt gaagttgttt taacaccaga acaaatccaa
      301 accttgcgtg gttatgcttc ccgtggcgat acctatggcg gttggcgtta tttggctaat
      361 ttgggtgacc gttatgcgga tgatgctgct gcaattgtcg gtaaggatgc aaacttaaat
      421 ggtttgaatt tatggatgaa aaaaggggtg gaaaacctat gggatgatac ggtcggtaaa
      481 aagacccgtt taatgtgtat ttccgttttt tggattgtgg ttttcaattt gtagcgaatc
      541 ggattcggca tatacggcat tgcaaaaagc gtttgactct ccaatgccgt ctgaaaaccg
      601 gtttcagacg gcatttgcgt tcagtgagaa aggtcgcgcc tgccgcccga acgtctcgcc
      661 gcagcctctg cataacggct cacctctttt tccaaatttt ccaagttcaa aggaaaatca
      721 ggcagtctgt ctccctgttt ctcttcgcgg ataatccgcc cgccatccaa ataccacgtc
      781 tgttgcgcat gataggtctg tatatccgcc gttacgccat ccgctttcaa tgctaccgtc
      841 gaagattgtg caataaaaag atttccgttt ttcaaataat attcgaaact ctggcgtttt
      901 tttccattgt cgaaactcca atagactttt tgcggcagac cgtccgcatc atagccgacc
      961 acaagactgt tcgccttcat cccccggggc atcaattccc gcatattctg ataaaacaca
     1021 gaattgcgcg agtccgacgc aatccggttg ctctcttcgc ggaagtccca aaccttctgc
     1081 tcgtcattcg caacatcccg gtatttcgcc aaatatacct gggccatctg ataacacccg
     1141 aggcaatgct cataaacatc ttccccgatt tttccgcgcc ccgacgcatc aaataccgaa
     1201 ccgtccggtt gccaaacaac ccgatattct cctgtcgttt cataattttc tccgtgagcc
     1261 gttccgccgt acacatttac agaaaacgga cgatcgttcc gatacagata ttcggcatta
     1321 acaaacgctt ccggcgagcg ttgcaaaagc gaaaccgcaa ccaaaccgcc ctcgccgatg
     1381 tagtaattca gccaaacctc ttccccatgt tcctgctccg ttacgtgaaa ccatttcgcc
     1441 ttttctttca aacgactgag ccggacagcg agcgcgagat aatccttctc cgactgcaac
     1501 ggaccgtcat ccacagctcc ggcaagactt tcctccgtcc tcatcgattc cttcacgatg
     1561 acaaccgccc tgtcggcatt tcggaacagg cgggcaagtt tcgccacaaa agcattcgga
     1621 ttttcaggta tttcagttgc cgtatcgctc aaaaaccaac gcggattaat ctcataggca
     1681 atacccgtcc ccagccaaaa ggcaaataca agggcaaaaa atgacaacaa taccagtttg
     1741 aattttttaa acatatttat ttttcgttta attcgtttaa caggatatat cgattatatc
     1801 agacgagctt tgattgtttg atttttaatc taatctacgc ttgctgccgt actttaaaaa
     1861 aacctatgca ggctgtggct tgttactctt gtttgctttg attaaaattt tcaactaatt
     1921 caaatgctta agatcgatat tgcctttgcc atttttccca atctaacggt tttcccgagc
     1981 gttcccaatc ttctaaaacc gacattttga acatttccat ttcccaccaa tattcggcat
     2041 ataaacgtga ttgattggga atgtcggctt ctgcccaatc ggcaaaatgc aaaaaatgag
     2101 aaatatcgca aactatatta ttttcaggaa cttcttcggc aaatagatgc ttgactgact
     2161 gtaacgcttc ggtctgatat ttttctgtcc atttctccca tttttcaggc tttcccgata
     2221 tttcccaatc ttccaatgcg gaagcattca agatttccaa ttcccaccag caatcgtaat
     2281 aacgagagga ttggttagga attttatttt ccgcttcgga aacaaattgg gtaaaactaa
     2341 aaatatcctt aacgattttt gcaaatctat tttccataaa ttgctgcctc ttccaaattg
     2401 cgtgccaaca acttcggcaa aatcaagccg gcaagcccct gcattctgtg ccaatcggag
     2461 aaaatgccgt ctgaaagccg aataccgctt cagacggcat tttgattgtt gggtttttaa
     2521 tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt cgagctgata
     2581 acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt cttttcaaaa
     2641 aaatcaaaac tttcttcttt caaccagtcc atataactgt catctattat ctcgaaaaaa
     2701 taatttgcag taggattccc atctgaatta ttttcttctt tattttcaaa taaatattct
     2761 gaaaatttta ctttataaga ttcatctgtt atttgaaaat gaattatatt gtcaataaat
     2821 atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc ttgttcggta
     2881 acacaaattt tgtccacata aaatctgtga ttattgttag aaaattgtaa tggttt
//
LOCUS       DAWGJV010000185         2936 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWGJV010000185 DAWGJV010000000
VERSION     DAWGJV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.185"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(399..1352)
                     /locus_tag="WHQ94_001920"
     CDS             complement(399..1352)
                     /locus_tag="WHQ94_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129148.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            1735..2754
                     /gene="galE"
                     /locus_tag="WHQ94_001921"
     CDS             1735..2754
                     /gene="galE"
                     /locus_tag="WHQ94_001921"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ2129149.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLAQMMEDSWRWVGNNP
                     NGYDD"
     gene            2870..>2936
                     /locus_tag="WHQ94_001922"
     CDS             2870..>2936
                     /locus_tag="WHQ94_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ2129150.1"
                     /translation="MRKILVTGGAGFIGSAVVRHII"
BASE COUNT          862 a          586 c          567 g          921 t
ORIGIN      
        1 ggtatttggg gcgaaaacag ctgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
       61 ggaattttgc aaaggtctca ggctgcattt ctcaatgtaa aacgcgatat tttcacccat
      121 tattagggat aagtagccag tggtttacac tggctacttt catagttatg aagtactggg
      181 tctgagtgaa ttcaattatt tccgtataaa acacagtaga cattttacta atcggcaaaa
      241 ttatattaga agaattgaga actactgaag tcaggtaaga taccattttg cacaaattta
      301 acggcatccc taaaaagcat tttgggtggt atttaaagga gtacaaaatg gttttaacta
      361 cagtatgctt agaaatctat agccttacgc gctttatatt aaatcacctt tctcaaccat
      421 ttagaatcat aaaaaaactt tttaggatcc ctaattaact ttttaaattt tcttaaaaaa
      481 atagttttag ttgattctat actattcacc gcagataaat attcttggta atcatgttgc
      541 aatggcacaa cctgctttaa tagcttatta aactcatcag ttgtaatcac ctttttagta
      601 gcatagcaat caagccaatc cggatctgct atttttgggg aagtgaaatc tatttgatca
      661 aaataaacta aaaccccact acctaaaaaa tgctgatttg cataggattt agaaaagcct
      721 aagtctgcaa caacaccaac agggataccc ttacattcag cttcaaaaag catagtagat
      781 gaataagata aacaataccc catttcttgg aaggcttgcg cggtactttt tcgctctatt
      841 gtcaaattac tcggcaactg aaactgcttt gccagctcta tatacgaatg tttatcctga
      901 tgcacagtaa tatctttatc tgcaaccctt agcaaaatag taaattcttt ctcagggtat
      961 ttttcagcca aggcaatcaa tttttttaat gtataaattc tttcttcttt tttaaatggg
     1021 attttaactt ggtcaataaa gtaaattttc tctccatgac gaccttttga tgcgtggcga
     1081 aaaattggat aaccataaag tatattttga caacttaatt tatattgttt cttatattcc
     1141 tctgcaattc taaaatcatg cttattatta tataaaacaa tatcagcccc catacgagat
     1201 agaatacttg cctgatcacc gaatactaca cctggaaaca aggtaataat caatggcctt
     1261 gaagcaatat ttaattgcgc attctgctta aagaaccttt ttaacaaccc atttccaacc
     1321 gataaaatta ctgcgtcata atattgatgc atattcttaa taaagtaatc atcaatatga
     1381 aaaaaaaaat tgcttggtct ttatctatcc ctgattctaa taactgtcga tttgaaagaa
     1441 tgttttctcg acttttgtgg atataaatat caatttgagc atcttttatc tcttttgcaa
     1501 cagcatagcc cgagttaagg aacgagtcat aactggcaat tagtaatact tttttcacta
     1561 ttttccctta attctacacc atatgaataa aattatagta ttttacctta gatatcaaat
     1621 atacttaacc aaaaaaatct gaagaaatct actgctactt cagggataat tatttcatta
     1681 agattttaca accattatta tcaatctatc aaagtatttt tagtatttta ttctatgaaa
     1741 aaaattctcg ttaccggcgg caccggtttt atcggctcgc ataccgttgt ttctttgctg
     1801 aaaagcggcc atcaagtcgt gattttggat aacctatgca attccagcat caatatcctg
     1861 ccacgcttga aaacgattac cggccaagag attccgtttt atcaaggcga tatccgcgat
     1921 cgtgagattt tgcgccgtat ttttgcggaa aaccgcattg attcggtgat tcattttgcc
     1981 ggcttgaaag cggtgggtga aagtgtggcc gagccgatga aatattatga taataatgtt
     2041 tccggcagct tggtgttggc ggaagaaatg gcgcgtgcgg gcgtgtttag cattgtgttc
     2101 agttcttcgg cgacggttta tggcgatccg ggcaaagtgc cttataccga ggatatgcca
     2161 ctgggcgata ccaccagccc ttacggcaca tcgaaatcaa tggttgagcg cattctcact
     2221 gacattcaaa aagccgatcc gcgctggagc atgattttgt tgcgttattt caatccaatt
     2281 ggcgcgcatg aaagcggctt gattggcgag cagccaaacg gtatcccaaa taatttgttg
     2341 ccttatatct gccaagtggc ggcaggcaaa ctgccgcaat tagcggtatt cggcgatgac
     2401 taccctaccc ctgatggcac gggtatgcgc gactatattc atgtgatgga cttggctgaa
     2461 ggccatgtcg ccgccatgca ggcgaaaagc aatgtagcag gcacgcattt gttgaactta
     2521 ggttccggtc gcgcttcttc ggtattggaa atcatccgcg catttgaagc ggcttcgggt
     2581 ttgacgattc cgtatgaagt caaaccgcgc cgtgccggtg atttggcgtg cttctatgcc
     2641 gacccttcct atacaaaggc gcaaatcggc tggcaaaccc agcgtgattt ggcgcagatg
     2701 atggaagact catggcgttg ggtcggcaac aacccaaatg gctacgacga ttaagttgac
     2761 ctgatacagg ccgtctgaaa gagatgtttt cagacggcct ctttatctga aaaacacaca
     2821 ttctgtctgc tataatctgt ttatattttt tggctatcct ctgaaattta tgagaaaaat
     2881 ccttgttacc ggcggcgcgg gctttatcgg ttctgccgtt gtccgtcata ttatcc
//
LOCUS       DAWGJV010000186         2827 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWGJV010000186 DAWGJV010000000
VERSION     DAWGJV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.186"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(36..512)
                     /gene="greA"
                     /locus_tag="WHQ94_001923"
     CDS             complement(36..512)
                     /gene="greA"
                     /locus_tag="WHQ94_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ2129151.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            907..2208
                     /gene="aroA"
                     /locus_tag="WHQ94_001924"
     CDS             907..2208
                     /gene="aroA"
                     /locus_tag="WHQ94_001924"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ2129152.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVARMHERPIGDLVDALRIAGTDVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTRQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FAHELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2246..2779)
                     /locus_tag="WHQ94_001925"
     CDS             complement(2246..2779)
                     /locus_tag="WHQ94_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ2129153.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMATASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          601 a          775 c          766 g          685 t
ORIGIN      
        1 cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac ttcgataatg
       61 tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatccccctc ttccttgccg
      121 attaaggcgc gggcaatcgg tgagccgaca tagattttgc cctgtttgat atccgcttcg
      181 tcttcgccga caatttgata ggtaacgtgt tcttccgttt ccaaatcttc cagcgtaacc
      241 gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat aatatgggca
      301 acggaaagtt tatgttccaa ctcggaaatg cggccctcga taaaaccttg gcgttctttg
      361 gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg aacgggcttc ggcaatcgct
      421 tcgatcactt cgggacgcgc cacgcttttg agctgctgca attcctgttt cagcaattcc
      481 gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata ttccggcaca
      541 ccggtttgcg gcagcaaaca taccgcgtac cgtcttgttt tgtgcgtccg gatattaaaa
      601 taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag cggctattct
      661 accaaattct atgaaattgg caatcgtgcc gcgccgccgg caaacgcgcc atatccgcaa
      721 caaaagctga aaatatgccg acaaagaaat tttagaaaca aaaaatttaa aaataatcaa
      781 ttttcggcat aaaaaaccac atttacggac tttaaaaccg aaaatgccaa acctgagatt
      841 tttcatacgg catttgcacc agtataatgc aggctgtttt tatctttata atattgacgt
      901 tttgccatga ccgaatccgt ccgcctcccc gtcgcccgtc tcaaaccttc caccgtcgcc
      961 ctgcccggct ccaaaagcat cagcaaccgc accctgctgc ttgccgcctt gtccgacaat
     1021 gcttgcgaaa tccattccct gctcaaatcc gacgataccg accgtatgct cgaagcactc
     1081 gataaactcg gcgttgaaat cgaatatctt gccgaagacc gtctgaaagt gcacggcacg
     1141 ggcggacgct tccccaaccg cactgccgat ttgtttttgg gcaacgcggg cacggcgttc
     1201 cgcccgctga ccgccgcgct tgccgttttg ggcggcgatt atcatttgca cggcgttgcg
     1261 cgtatgcacg aacgccccat cggcgatttg gtcgatgcgt tgcggattgc cgggaccgat
     1321 gtcgaatatc tcggcaagga acactatccg ccgcttcata tcggcgaacg ccaagacaac
     1381 ggcgagcgcg tgattccgat taaaggcaat gtgtccagcc agtttctgac cgccctttta
     1441 atggcgttac cgctgaccag gcaggcgttt gaaatccgta tggtcggcga gttaatttcc
     1501 aagccctata tcgacattac tttaaaactg atggcgcaat tcggcgtaca ggttatcaat
     1561 gaaggctacc gcgtcttcaa aattcccgcc gatgcgcact accacgcgcc cgaacacttg
     1621 cacgtcgaag gcgatgcctc tagcgcgtcc tacttcctcg cagccggttt gattgccgcc
     1681 acgcccgtcc gcgtgaccgg catcggcgca aacagcatac agggcgatgt cgcctttgcc
     1741 cacgaactgg aaaaaatcgg ggcggacgtg gtttggggcg aaaacttcgt cgaagtctcg
     1801 cgcccgaaag aacgtgccgt ccaagccttt gatttggatg caaaccatat ccccgatgcc
     1861 gccatgaccc tcgccatcgt cgcgcttgcc acagggcaaa cctgtacgct gcgcaatatc
     1921 ggttcatggc gcgtcaaaga gaccgaccgc atcgccgcaa tggcaaacga gttgcgcaaa
     1981 ctcggtgcaa aagtcgccga agaagccgaa gcgattcaca tcaccccgcc tgaaacgctg
     2041 acgcccgacg ccgtcatcga cacttacgac gaccaccgta tggcgatgtg tttctcgctg
     2101 gtttccttgt tgggcgtacc cgtcgtcatc aacgacccga aatgcaccca caaaaccttc
     2161 ccgacttatt ttgaagtgtt ctcatcgctg accgaaacag cggaataagg cggcattttg
     2221 ccgcgattcc ggcgcggcgg gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt
     2281 ttttggcgta aaacggtgtg gagagttttt cggttttgat ggttttgccg ctgctggggg
     2341 catggatgaa ttcgccgtta ccgatataga gtccgacgtg tgagtagcgg tgtgcgccgc
     2401 cggtgttgaa gaatacgagg tcgccggcct taaggcggct gtcggggatt ttgcggcttg
     2461 ccgtcgccat gtcgcgggcg gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga
     2521 attgaatcat gccgctgcaa tcgaagccgg ttgcggtgct gctgccgccc catttgtagg
     2581 gcgtgccgat gagtccgagg ctgtggagca tgagttcctg cgagccttgt gtgcggtcga
     2641 tgtggctgat gcggacggct tggatttgcc ggactgtctg tttgggtttc ggttggcggt
     2701 gtttgccgga ggtcgtgccg catgaggcga ggagcagtgc gctgagacag aggaaaaggg
     2761 ttttgtcggg gggaaacatg gtttttcctt tgcgggttcg gatatgccgt ctgaaggtgt
     2821 ttcagac
//
LOCUS       DAWGJV010000187         2663 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWGJV010000187 DAWGJV010000000
VERSION     DAWGJV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.187"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(461..2095)
                     /gene="groL"
                     /locus_tag="WHQ94_001926"
     CDS             complement(461..2095)
                     /gene="groL"
                     /locus_tag="WHQ94_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ2129154.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(2188..2478)
                     /locus_tag="WHQ94_001927"
     CDS             complement(2188..2478)
                     /locus_tag="WHQ94_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ2129155.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          552 a          696 c          709 g          706 t
ORIGIN      
        1 tccgggcaga attaaggggg tatttgggta aaattaggcg gtatttgggg cgaaaataga
       61 cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcaa
      121 acaaaaacag aaacctaaag tcccgtcatt cccgcgcagg cgggaatcca gaccaccaac
      181 gcggcaggaa tctatcggaa ataaccgaaa ccagacgaac ctagattccc gctttcgcgg
      241 gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt cgtttttggg
      301 atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaaca acaatgcaaa gaacggcgtt
      361 ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaaccg cacggcgttg
      421 accgtgcggc ttgttgtctg aaggcttcag acggcattgc ttacatcatg ccgcccatac
      481 cacccatgcc gcccatatca ggcatagccg gtttgtcttc agggatttca gcaatcatgc
      541 agtctgtggt cagcatcagg ccggcgatag acgcggcgtg ttgcagcgcg gaacgggtta
      601 ctttggcggg gtcgagtacg cccatttcga tcatgtcgcc gtattcgccg ctgccagcgt
      661 tgtaaccgta gttgcctttg ccttccaaca ctttgttcac aaccacgcta ggttcgccgc
      721 ctgcgttggc aacgatttgg cgcagcggag actcaacggc gcgcaagacg atttgtacgc
      781 ctgcgtcttg gtcggcattg ccggtgtgca ggttttccaa agcggcacgg gcgcgcaaca
      841 gggctacgcc gccgcctgcg accacgcctt cttcaacggc tgcgcgggta gcgtgcagcg
      901 cgtcttccac gcggtctttt ttctctttca tttcgacttc ggtagcggcg ccgactttga
      961 tcactgccac gccgcctgcc aatttagcca cgcgctcttg cagtttttct ttgtcgtaat
     1021 cgctggttgc ggtttcgatt tgttggcgga tttcggcaac acgcgcttcg atttgggctg
     1081 cgtcgccaaa gccgtcgatg atggtggtgt tttctttacc gatttcgatg cgtttggctt
     1141 gacccaagtc ttccaaagtc gctttttcca gagacaggcc gacttcttcg gaaatcacca
     1201 cgccgccggt caggatagcg atgtcttgca gcatcgcttt gcggcggtcg ccgaagcccg
     1261 gagctttgac ggcaacggtt ttcaggatgc ctcggatgtt gttcaccacc aaagtcgcca
     1321 aggcttcgcc ttctacgtct tcagcgataa tcaacagcgg acggctggct ttcgccactt
     1381 gttccaaaac aggcagcagg tcgcggatgt tgctgatttt tttgtcaaac agcaatacaa
     1441 acggattgtc caaagcagcg atttgttttt cagcatcgtt gatgaagtaa ggggacaggt
     1501 agccgcggtc gaactgcata ccttcaacca cgtccagctc gttttccaaa gatttgccgt
     1561 cttcaacggt aatcacgcct tctttgccga ctttttccat cgcttcggcg ataatcgcgc
     1621 cgacttgttc gtcagagttg gcggaaatcg agccgacttg ggcgatttct ttggaagtat
     1681 cgcaaggttt ggcgatgttt ttcagctcgt caaccaaagc ggcgacggct ttgtcgatac
     1741 cgcgtttcag gtcggtcgga ttcatacctg cggtaacata tttcatgcct tcggcaacga
     1801 tggattgtgc cagtacggtg gcggtagtcg taccgtcgcc cgccacgtcg ttggttttgg
     1861 acgcaacttc tttcaccatt tgcgcgccca tattttcaaa cttgtctttc agttcgattt
     1921 ctttggcgac ggttacgccg tctttggtga tgtgcgggcc gccgaaagcg cggtcaacca
     1981 ctacgttgcg gcctttggga cccagggtta cgcgcacggc gtttgccaaa atgttcacgc
     2041 cgtttaccat tttttggcgg acttcattgc cgaactgtac gtcttttgct gccatttcaa
     2101 ttctccaaaa atcattaaaa ctgtctgata aaaccgttta tgccgtctga aggcggtttg
     2161 ccgtttcaga cggcatcgtg tccatattta tttttcaacg atgccgaaga tgtcttcttc
     2221 gcgcatcacc aacagctctt cgccgtcggc tttgacggtt tggccgctgt atttgccgaa
     2281 gatgattttg tcgccgactt tgacatccag cggacggcgc gcgccgtctt taccgatttt
     2341 gcccgcgccc acggcgatga cttcgcccat atcgggtttt tcggcggccg cacccggcaa
     2401 aacgatgccc gatgcggttt tttcttcagc ttccaagcgt ttgacgacga cgcggtcgtg
     2461 taaaggacgg atggtcatat ttatgctccg ataaaatggt ttgaaaacaa tcatctgccc
     2521 gaacggttca ggcagattga agtggaaacc ggacagccgt caggcggctg tccgtataag
     2581 tcggcaaaat tagggtgtgt gcgggtaaat tcaagtgagg cggaaaaaat ttatttccgc
     2641 cgttttttat agtggactaa att
//
LOCUS       DAWGJV010000188         2655 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWGJV010000188 DAWGJV010000000
VERSION     DAWGJV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.188"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..888
                     /locus_tag="WHQ94_001928"
     CDS             <1..888
                     /locus_tag="WHQ94_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2129156.1"
                     /translation="LTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQPDLVVYG
                     NYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRPGYWRG
                     HTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLLNNTLR
                     PTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNGNVSYF
                     GKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRGQYDR
                     SFAVVGMPRTYWLKAELDF"
     gene            1012..2562
                     /locus_tag="WHQ94_001929"
     CDS             1012..2562
                     /locus_tag="WHQ94_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ2129157.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKIGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT          755 a          618 c          669 g          613 t
ORIGIN      
        1 ctgactttgg gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacagagc
       61 ggcgatgcca aacgcttcag cccgatgctg atggcggcgt gggtgccgca acctgatttg
      121 gttgtgtacg gcaactatat ggaagacttg gagcctgccg atatcaaaac cgatgattcc
      181 ggcgaaacga caatggcgaa accgcgtgtc agccgtcagt ttgaagtggg cgtgcgtaaa
      241 aactggggca atttcgtaac cacgttaaac gcgttccaaa tcaaacgccc gggctactgg
      301 cgcggtcata caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac
      361 aattccgatt tcgcccgcta taaggcgcaa ggcggcgcgg ccggcgacga gcaaggtatg
      421 gaacgcaatc gcggtattga gttcaacacc tacgcgaact tgctcaacaa tactttgcgt
      481 ccgactttgg gtctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat
      541 atgctggtta acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaagc aggtttggaa
      601 tgggatacac cgtttgccaa aggattgact ttaaacggta atgtttcgta tttcggcaag
      661 tcttaccaag acacgcaaaa acaatacgcc ttcccgtcct ataccttgat tgacgtaggc
      721 gcgcgctaca aaaccaagct cggcaaaaat actttgaccg tcagcagctc ggtggaaaac
      781 ctgttcaaca aaaactattg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc
      841 gtcggtatgc cgcgcactta ctggctgaag gcggaattgg atttctgatt tccctatatc
      901 cggaaaatgc cgtccgaaca ggtttcagac ggcatttctt ttaaagagat atggcgggat
      961 acgcttaaat aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa
     1021 cctttgattt cggttgcggc agcattgctc ggcgttgctt tgggcacgcc ttattatttg
     1081 ggtgtcaaag ccgaagaaag cttgacgcag cagcaaaaaa tattgcagga agcgggcttc
     1141 ttgaccgtcg aatcgcacca atatgagcgc ggctggttta cctctacgga aacgacggtc
     1201 atccgcttga aaccggagtt gctgaataat gcccggaaat acctgccgga caacctgaaa
     1261 acagtgttgg aacagccggt tacgctggta aaccatatca cgcacggccc tttcgccggc
     1321 ggattcggca cgcaggcgta cattgaaacc gagttcaaat acgcgcctga aacggaaaaa
     1381 gttctagaac gcttttttgg aaaacaagtc ccggtttccc ttgccaatac cgtttatttt
     1441 aacggcagcg gtaaaatgga agtcagtgtt cccgccttcg attatgaaga gctgtcgggc
     1501 atcaggctgc actgggaagg attgacggga aaaacggttt atcaaaaagg tttcaaaagc
     1561 taccgcaaca gctatgatgc gcccttgttc aaaatcaagc tggcagacaa aggcgatgcc
     1621 gcgtttgaaa aagcgcattt cgattcggaa acttcagacg gcatcaatcc gcttgctttg
     1681 ggcagcagca atctgacctt ggaaaaattc tccctagaat ggaaagaggg tgtcgattac
     1741 aacgtcaagt taaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc
     1801 aatcccaacg gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc
     1861 aagatcgggg aatcaggctc atttatcaac agtgaagggc agttccgttt tgacacgctg
     1921 gtgtacggca atgaaaaata cggcccgctg gacatccata tcgccgccga acatctcgat
     1981 gcttctgcct taaccgtatt gaaacgtaag tttgcacaaa tttccgccaa aaaaatgacc
     2041 gaggaacaaa tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc cggactgttc
     2101 accaacaatc ccgtattgga cattaaaact tttcgtttca cacagccgtc gggaaaaatc
     2161 gatgtgggtg gaaaaatcat gtttaaagac atgaagaagg aagatttgaa ccaattgggt
     2221 ttgatgctga agaaaaccga agccgacatc agaatgagta ttccccaaaa aatgctggaa
     2281 gacttggcgg tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa
     2341 ggcagggcaa gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt
     2401 cagagtatgg caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt
     2461 tctctgaaaa acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagccg
     2521 gattttgatg aaggcggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg
     2581 aaatgttcag gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat
     2641 aagccgtgtc ggatt
//
LOCUS       DAWGJV010000189         2633 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWGJV010000189 DAWGJV010000000
VERSION     DAWGJV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.189"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..458)
                     /locus_tag="WHQ94_001930"
     CDS             complement(<1..458)
                     /locus_tag="WHQ94_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ2129158.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVYETFKEDIQAQGYQY"
     gene            complement(805..1107)
                     /locus_tag="WHQ94_001931"
     CDS             complement(805..1107)
                     /locus_tag="WHQ94_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129159.1"
                     /translation="MGYRVGMNCFDTRLQADDYLLSSLPPTVTEEGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
     gene            complement(1135..1365)
                     /locus_tag="WHQ94_001932"
     CDS             complement(1135..1365)
                     /locus_tag="WHQ94_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129160.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDAAAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1603..1803)
                     /locus_tag="WHQ94_001933"
     CDS             complement(1603..1803)
                     /locus_tag="WHQ94_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129161.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1807..2118)
                     /locus_tag="WHQ94_001934"
     CDS             complement(1807..2118)
                     /locus_tag="WHQ94_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129162.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(2130..>2633)
                     /locus_tag="WHQ94_001935"
     CDS             complement(2130..>2633)
                     /locus_tag="WHQ94_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation factor family protein"
                     /protein_id="HEZ2129163.1"
                     /translation="EIPLDILINQGSYFCGAFPICRKFKNMPVSERFDQRKKTLNLTF
                     EHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLRDGLKH
                     GFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLSKVYHQ
                     NVDYDYF"
BASE COUNT          839 a          595 c          396 g          803 t
ORIGIN      
        1 tcgtattggt agccttgtgc ctgtatgtct tctttgaaag tttcgtatac gtcgtgggct
       61 aaaagggctg ttccgacata gggaaccgcc cttgtgctga atttcgcgcc taagcgggca
      121 agtttgccga ctcctgccaa gactccggcg cgggaaactg atgaagatac aaccgaacta
      181 actttacccg tacttgatgc tccagtagat acatgcctaa cttttaaatc ttgagcttct
      241 gaagttaaat atcttcccga ttctctatca aatacacctt tccaaattct tcttcttttg
      301 tcatctgaac ctcgaatacc taaatatctg ccatcatcat aaacttcaac cctatcaaat
      361 ttacccggaa catttactga tgccaatgca aatttaggta caaaaaaact aaagcatagg
      421 ataatcttta aaaaatttcg ccctaggata agaaacataa cattttgctt catctaattt
      481 aattaataat gtagaagaat cagggaattg aatttctata aaatctctat cataacgatg
      541 aaagaaattc ctaaaaatat caaatgaaaa cgaagtttta aaaacccctt ttctaatcaa
      601 ttccctataa taaattaacg cttgtggtaa atctctataa atatgtgaat cagtttgaaa
      661 tttaactaaa tgatattcag aaataaacat aattcggacc ttttaaaaca ctctaaaaag
      721 ccgattctac caaccgttac caaaacagcc aagcaacaga caaagccaag aagaaaccga
      781 gaagaaacca aaaatcaata atcatcaatc agtaccaacc ttgcccatgt cttttaagaa
      841 attaatcaga agcctgaaac cgaaaatgac tacgaacaga attagaaccg tcgaaccgac
      901 ataagaacct tgctttatct gctcaaaatt ggaacattcc ggataagaca acgtaaccgg
      961 ctttccgttc aaaatccatt tatcgcccac cctttccggc ctgatgattt ttccttcttc
     1021 tgtaacagta ggaggaagag acgacaataa atagtcgtct gcctgcaatc ttgtatcaaa
     1081 acagttcatg ccgacacgat aacccattca cgcgcccctc tttcttcact gccgttattt
     1141 gacagattta atcatgctcc aagccatttt gaagccttgg attgccaaaa tgacggtaat
     1201 ggccgccata cccacggcgg aaaccatcgc aacgaaaccc atgattacat tcgccacttg
     1261 cgtaccaatc gcggctgcat caaagccatc tgccataaca atagccggtg tgaagatacc
     1321 agctgccaac aatgctttta cagcatattt tttaacgatg ttcatcgttt ttttcctttt
     1381 tttgatattt aaaataagac gacttcttga cttgcttcat ccggacgaag tcttttccga
     1441 atctcgtttt tagccgataa aatagaggat tgcgaaaaga aaaagaaaca tacagaccac
     1501 ccagccgata attagtgttg caaggttcat tttcatgata tttttccttt gttgcgggct
     1561 ttgtgaaagg ttaacagacc gcccgccgag cctgtttttc ttttattccg attttacgaa
     1621 gaactgaaat atctggaatc ctccgcccat ttcatttatg cctgaattca acgcatcttc
     1681 gtaactttca aattgacctg ctgatttaat attttgagta aaccccacat caccgaacgg
     1741 atcgggataa ataaagtcat gcgtttctaa atcttgaact atgaaacgtt cttcaaattt
     1801 cataaatcaa cctttcggct tttccgccac ctgaaaatca attaatgacg gaaccatgcc
     1861 tttacctgtc gaagtcattt caaccattac cataacttcg cacggatatt tgagattctc
     1921 taaccttgag aaattcttac tatccccgaa cttcatttgt gctgccgtaa atccaacagc
     1981 attacccgac tgtgccggta aaggtgttgc aaccaatacg gaacaagtgt caatgttaga
     2041 gccatcaatt tcacctttga attttttggc tcccaaaaaa gttgcaggat aagttacagt
     2101 ttgagtttga ttaaacattt tgatttcctt taaaaataat cataatctac attttgatga
     2161 taaactttac ttaaatattc ctgatatttc ctttctttct cgacatcata atcaggacta
     2221 aaaagccttt tttccctatc gaatttgtca gaatttttaa aagcaataac ccccaattca
     2281 tcaagttcaa tttccaaatc aatatccggc tgttcatgaa taaaaccgtg tttcaaaccg
     2341 tcccttaaca tttccagagc atatttttca ggttctaatc ctttgggaaa tcccgaatct
     2401 gcctttaaag attccacaat ttcgctatta tcaaaaccca tttcaatcat gaaattgacc
     2461 agttttccaa ccgcgttttt cgcgtaatgc aatttatgct cgaaagttaa attaagcgtt
     2521 ttctttctct gatcaaacct ttcggaaacc ggcatatttt taaattttct acaaattgga
     2581 aaagctccac agaaatacga accctgattt attaaaatat ccaagggtat ttc
//
LOCUS       DAWGJV010000190         2623 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWGJV010000190 DAWGJV010000000
VERSION     DAWGJV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.190"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(68..364)
                     /locus_tag="WHQ94_001936"
                     /pseudo
     CDS             complement(68..364)
                     /locus_tag="WHQ94_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(336..572)
                     /locus_tag="WHQ94_001937"
     CDS             complement(336..572)
                     /locus_tag="WHQ94_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129164.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            complement(575..739)
                     /locus_tag="WHQ94_001938"
     CDS             complement(575..739)
                     /locus_tag="WHQ94_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129165.1"
                     /translation="MGQVEFYEKMIELWSSKSREASERADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            complement(742..948)
                     /locus_tag="WHQ94_001939"
     CDS             complement(742..948)
                     /locus_tag="WHQ94_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129166.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(945..1490)
                     /locus_tag="WHQ94_001940"
     CDS             complement(945..1490)
                     /locus_tag="WHQ94_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2129167.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(1647..2075)
                     /locus_tag="WHQ94_001941"
     CDS             complement(1647..2075)
                     /locus_tag="WHQ94_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ2129168.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(2056..2490)
                     /locus_tag="WHQ94_001942"
     CDS             complement(2056..2490)
                     /locus_tag="WHQ94_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ2129169.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          613 a          772 c          555 g          683 t
ORIGIN      
        1 aaaacagccg aaaacctgtg tttgggtttc ggctgtcggg agggaaagga attttgcaaa
       61 ggtctctttc cgttttcaga cggctgactt ccgcctgttt tttcgccaaa gccatgccga
      121 cagcgtgcgc cttgacttca tattttttcg cttccgcgcg tgccagttcc agttcgcgcg
      181 catagttttg agccgacaac agcagggctt gcgccttatc gtgctccatc ttgtcgatga
      241 ccgcctgctg cttcgcaaag gctgccttgt agccttgatg gtgtgacacc gccaaacccg
      301 tgccgacaag cgcgatgatg gcaatcggct gccagttatt cgccagcagt ttcacgagat
      361 tcattctcga cctcctgacg tttgacgctg acaaacgagc gcgccacggc atagccgccg
      421 acaatgccca aatacaccgc ccaaatctcc gccgacgggt cgggcaacat cacaaattta
      481 aacgtccccg ccgcgcaggc gacgtttgcc cacagcttcg agtgcgacac attgcctgtc
      541 gccgggtttt taaaaatatc caaaatacgc attgctattc cacacttttg gtttgcaggt
      601 gccgtttcag catttcccga taattggcca gttcgccctc cgcaaattca aacgccgcca
      661 aatcagcccg ttcgcttgcc tcacggcttt tgctcgacca cagctcaatc atcttttcgt
      721 aaaactcaac ctgtcccatg attaacgacg attcttgcgt ttgcgcgccg cgcgtttggc
      781 tgccgccacg cctgagtttc ttaaatttgg gcgtggatag gtgtgtatcg gacgaacatt
      841 tggcatctgt ataagtgcgc ggtttacctg ctcattacta ggattttctc caatcaattt
      901 tgtaagaaaa taggaagcag cttctttcaa tttaccaata atattcatac tcttgccgcc
      961 cccaattcca tcgcaatcgc gtccgcaatc gcgcggcaga tgccccattt agtagtctta
     1021 aacaaggcta aatcagtgtc gttgctgata aaaaacggct caaacacaat gccgccggct
     1081 tgtgcataag ccaggcgcga atgctggccc gcattgtcgg gtttaaagcc gtcttcgccg
     1141 cgcagtttcc agccggtttt cttggcaaca gctttgctca acacctgaca ccagcgtttg
     1201 tttttaacgg tactcaaggc ttcgatgcct gtcgccgctt tgctgacggc agcgttggtg
     1261 tgaaactcaa tcgccacatc cgagccgcga atcagcttga ccgcatcgcg caagggcata
     1321 ttgcctttgc cttcgccgtc ggtgcgtacg gtcaggccgt aatcgttacg caggattgaa
     1381 gccacgatgt tgcgcatatc ctgcgccaag tccgcctcac ggtcgcttcc gttgaccgcg
     1441 cccgggtcgg tgttactgtg tccggcggtt aaagttacag ttttgcccat tcatcatctc
     1501 caaaaaatat tgattgcata acatttgaaa accccattaa accgtcttta acccatcgtc
     1561 ccgaacggta gatgtttcat cccctacaaa ccaaaaaaaa agaccgtctg aatacagacg
     1621 gccaaagcct ggtcacacat cactgcctaa aataaagctg cctgctgtgc cgcaggtcgg
     1681 ctcatttcat tgataatcgt atatcctgtt cgtgaagaga taccgtattt agggcatagc
     1741 ttcgtcatcg ccataagccc gctctcctta tcaatatcgc gcaatttgac aaactcctga
     1801 taaaacctat ggtttctcaa ctgtattaac gccttgccgc accgtgggac atacaattcc
     1861 tcgccaccat atacctgcaa cagctcatgt gttttcactt cgccgatggc ttcgaccaaa
     1921 attgccaaac gctcggtgtc caccttgccc ttaccaaatt taaaccgcgc cccgccaatc
     1981 gccttgacca gctgttccgt cgctgccagt ccgatgacat ccacaatgtc caacacggta
     2041 tccggcaata aatgttcaac tttttcgaac gccatcatcc ccacccgctt tttccgtttt
     2101 cctgttttcc gcaatctgca acgcagcaac cagtttatgt agctgcgtat cgtctaaata
     2161 ttcgacctta tccttaccaa acatccgccg cgccattgcg tgtgcatagt tccaatgttt
     2221 gccgccgacg gtcagcaggg cttcgacttt gtccaacatt gccgctgatg atgtccgacg
     2281 cagatgcggt ttgccgtgtg ggttaccttt tgctttaggc ttaaatccgt gcgaccgcat
     2341 atcagcgaca acagactcaa gttcggaaac atccatatcc gcacacgacc gcttgcccgt
     2401 cacacgctcc aacaccgcgc gataggtacc gtcgtccaag cccagctcct tttgagcaat
     2461 cttaattttc gcaatcaacg cccggcgcat ctcaaaccca taaaacacaa tatattgact
     2521 aattaacgca tattataccg ataaaataca atatatagta ttaagccgat gttttttgcg
     2581 aaacggacag acataaaaaa gcaactgtat ttttcacccc gtc
//
LOCUS       DAWGJV010000191         2612 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWGJV010000191 DAWGJV010000000
VERSION     DAWGJV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.191"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(312..545)
                     /locus_tag="WHQ94_001943"
     CDS             complement(312..545)
                     /locus_tag="WHQ94_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ2129170.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WHQ94_001944"
     CDS             complement(711..911)
                     /locus_tag="WHQ94_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ2129171.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WHQ94_001945"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WHQ94_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ2129172.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WHQ94_001946"
     CDS             complement(2010..2450)
                     /locus_tag="WHQ94_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ2129173.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
BASE COUNT          697 a          745 c          522 g          648 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggtcaa tatgcaacac
      181 cgtagaagat tgacaattta ccaggcatcc aaacgtgctt cctttactgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgtctgaag tcctgcttca
      301 gacggcattt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagtt cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatc ctgaaaaggt
      481 ttgagttcgt cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccgc agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
     1021 ttggtaattt tttatcaagt tttcatttcc gattgatttc ttattaaacc gaagcagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacatt ggcaacatgg
     1141 gtgtaaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaatccgt gctgcaccaa gtgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgagaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcgccctt gcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 agtgccttgt cccgcaaacc gtgtggcgta tcagtatccg gagtggcaag cagccgggaa
     1621 atctgctgtt ctgtaatcag tgtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcac
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa cacttcctgt
     2041 gcatgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 ccaggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agacgcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca ca
//
LOCUS       DAWGJV010000192         2528 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWGJV010000192 DAWGJV010000000
VERSION     DAWGJV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.192"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            282..1409
                     /locus_tag="WHQ94_001947"
     CDS             282..1409
                     /locus_tag="WHQ94_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ2129174.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1439..2317
                     /locus_tag="WHQ94_001948"
     CDS             1439..2317
                     /locus_tag="WHQ94_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2129175.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTSRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMCPA
                     DEDIALIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            2321..>2528
                     /locus_tag="WHQ94_001949"
     CDS             2321..>2528
                     /locus_tag="WHQ94_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ2129176.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETEYSELNLN"
BASE COUNT          708 a          639 c          617 g          564 t
ORIGIN      
        1 atagtgtata ttaagtacgt ctgatatacg cgataccctc cgagggtgta aactttagtt
       61 cacatttaaa atgacctctt taaacctgcc tttcggcagg tttcttttta ggttgtttgg
      121 aaatcgtgtg cagacaaggt gtaaaatagg taacaacata aaataatgcg gttttaccgc
      181 ccatatattt acaaaagcca aatttttaaa catatattct tgatatatac acggcgtaaa
      241 catatactgg aaacatcttt aaattttccg aaattttaaa catgagcaac tggaaacccg
      301 atattcccta taacgattta ccacccctgc cgccaaaaca ggatattgaa agcaaaacca
      361 tcctgaaacg ttgtatagcc gcccgtgcat cccttgcccg tttaaagcag gcggcagaac
      421 tgataccgaa tcaagccatg ctgattaaca cccttcctgt tatggaagcc cgtgcaagtt
      481 cggaaattga aaacatcgta accacaacgg acaagctgtt tcaatccctg caaatggata
      541 cggaacggca agaccctgcc acgaaagaag ccctgcaata ccgcaccgcc ctgtttgcag
      601 gctatgaatc actgacgagc cgccctttat gcacacaaac cgccatcatg gtctgcaacg
      661 ccatcaagca cccctacgaa atggccatcc gcaaaacagg cggcacagcc ctaaaaggag
      721 gcaacagcgg aaatgttgtc tataccccgc ccgaaggaga agaaaccata cgcggcaagc
      781 tggcaaattg ggagcggttt attcacgaaa gcggcgattt agacccgctt atcatcatgg
      841 cggcggcaca ttaccaattt gaagccatcc atccgtttac ggacggcaac gggcggacgg
      901 ggcgcatatt gaacagcctg ctattgattg aaaaagggct tttggatttg cctattttgt
      961 atttgagccg ctacatcatc gaaaacaggg cggactatta ccgcctgctt ttaggcgtaa
     1021 ccgaacggca ggattgggaa agctggataa tctacatctt agacggcgta gccgataccg
     1081 ccgattggac ggtatcgaaa atagatgcga tacgccgcct gttcgagcag acacggcaac
     1141 acatacggac acacgcacaa ggaatctaca cgcacgaact ggtaaatctt ctgtttgagc
     1201 agccatatac acgcattgcc aacctagaag cggcagggat agccaaacgg cagacggcct
     1261 ctaagtacct gaaagagctt tcagacatag gtgtgctgca agaaatcgtc atcggcaggg
     1321 acaaactatt cattcatccg cgcctaatgg aactattgcg gggagagggc aacagcttta
     1381 cctcattcca atccctcgtt aaagcatagc caaaataatc aataatccgg aggtcaatat
     1441 ggcaagaagg tcaaaaacat ttgaagaagc tgctgctgag gttgaggaac gtttcggtca
     1501 tcgtggcatt aagttggtcg agtttgaggg tacagccaag ccgtgtgtaa tcaactgccc
     1561 taaacatgga aaccaaacct gttcgaggta ctccaatatg ttcataggaa gtagctgggg
     1621 ttgcccctct tgtggtaatg agcaagctgc aaaagccggt atagcgaccc ttaggaagaa
     1681 tcacatagcg ttagaaatgc tgaaacaggc tgtaacaggt atgaccaagc aagagcgcat
     1741 cacgacgcaa gcctacaatg agatgaccaa atccgtggca ggttcaaaca gcatagtcct
     1801 taacgatgtc caaggcgata cgaccatcaa caaccatcat acgcatacgc acaaccacag
     1861 cgatgccgat ggcaaagcac tgtcgatgag gctcacatcc cgtcctttgt tgtcagaccg
     1921 tcaggcggcg gctttcgccc gtacaggcaa actcacgggc agtttcgacc tgtttgcttc
     1981 ggtggtcgcc ccctcgcagt acacgtttgc cgttgccatg cccgacacgt ccatgtcgcc
     2041 ggttatcgaa aagggagact tgctggtggt cgagccgcgt atgtgccctg cggacgaaga
     2101 catcgcgctg attgaactgt ccgacaagcg gctggtcgtc gcgcaccttg ttatcgatat
     2161 tgcgggcagg atgctgattt atcagacggg caggccgtct gaagcctttg acctgcccga
     2221 aggcagcacg attttaggtg tggtgctgga ggtcaaaaaa cggtttatgt ccgccgcaca
     2281 ggcgagaagg cgtgttgatt cggattacca cccctgatgt gtggacggct ggtacgattt
     2341 ccgcttccaa aacgtcgtgt acgcgcccga ccgcagcccg gaaatcagcc gtatgctttc
     2401 ttcgattttg gcaggctacg cgtgggatac cgaaaacccg ttcgtggcga aatccgaaca
     2461 acgcctgtcg gccttgtccg aatgggtcgg tcagctggaa accgaatata gtgaattaaa
     2521 tttaaacc
//
LOCUS       DAWGJV010000193         2527 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWGJV010000193 DAWGJV010000000
VERSION     DAWGJV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.193"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(31..837)
                     /locus_tag="WHQ94_001950"
     CDS             complement(31..837)
                     /locus_tag="WHQ94_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ2129177.1"
                     /translation="MPTVFIFLMAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAVL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1057..2427
                     /gene="ffh"
                     /locus_tag="WHQ94_001951"
     CDS             1057..2427
                     /gene="ffh"
                     /locus_tag="WHQ94_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ2129178.1"
                     /translation="MLDNLTSRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAVGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          700 a          632 c          658 g          537 t
ORIGIN      
        1 ggggattcat acggcatctg ttggctcttc ttatcttttc agaatgattt ccaatacgaa
       61 cttgctgccc atataggcaa tcataaggct gacaaatccg atgatggtcc acacggcggc
      121 ttttttgccg cgccatgcgg tcatgctgtg cttgagcagc agtccgccgt aaatcagcca
      181 tgacaatatg ccgaatacgg ttttatgggt aaaggtcatg ggtttgccga atacggcttc
      241 ggcaaaaaat gttccgctga cgacggaata ggtcagcagg atgaaacctg cccacatggc
      301 ctggaacatg agtttttcca aactgagcag cgacggcagg aatcctgcga gcttggagaa
      361 gttcctgcgg tgcaggctcc gattcagcag cagggtcaaa acggacaata atgttgcgat
      421 gccgaacagc ccgtatgcga gcagcgaagt tccgatatgc agcataaagg gaaggtcggt
      481 aatttcatat cccgagaatt ttccaggaaa aaccaaacct gacagcagca tcagtacggc
      541 gcaaggatac agcagcaact gcactccgcg cagcggataa aagaagctgc cggcaaaata
      601 aataaacagc atcatccaaa caatcaggct gccggaatac ccgaagccca taatgatgat
      661 tttgtcttga atgaccggca taagcagtgc cgcgccgtgg acggtcaatg ccgcgcccaa
      721 aaccggcaat tccgtcttcc acgggtaatc ccggccgcgc ccctgctgtt ggcagtgcca
      781 tgcaaatgca cccaatcctg cgtaaactgc catcaaaaag atgaaaactg tcggcatggt
      841 ggactttctc tatctatact gttgcgccgt atgcggccgc ttatgaaata ttggaacttt
      901 taacgttgga attgtaaaat ccccgtttcg ggcaagcctt gacggatttg ccgatatgct
      961 gtccggcaca caagccgcat caaattattt tgattttatt ttaacaaaga atgcccctga
     1021 tggggcaagc tattcttatt cagaccaagg accagtatgt tagacaattt aaccagccgc
     1081 ttcagcaatg tcctcaaaaa catccgcggg caagccaaac tgaccgaaga caacattaaa
     1141 gaagccttgc gcgaagtccg actcgccctg cttgaggcgg atgtcgccct gcctgtcgtc
     1201 aaagagttcg tcaacaacgt caaagaaaag gccctcggtc aggaagtggc gggcagcctg
     1261 acgccggatc aggcatttat cggcgtggtc aaccaagcct tagtcgaact gatgggcaag
     1321 gaaaacaaaa cgctggattt atcggttgcg ccgcccgccg tcgttttgat ggccggtttg
     1381 cagggcgcag gtaagacgac gactgtcggc aaactcgccc gcctgctgaa aaacgatcag
     1441 aagaaaaaag ttttggtggt atccgccgac gtttaccgcc ctgccgcgat tgaacagctg
     1501 cgtctgttgg ccgaacaggt cggcgtggac tttttcccat ccgataccaa ccaaaaaccg
     1561 gttgaaattg caactgccgc cgtcgattac gccaaaaaac atttttacga tgtattgatg
     1621 gtcgataccg ccggccgttt ggcaatcgat gaggagatga tgaacgaaat caaagccctt
     1681 catgcggcgg ttaacccggt ggaaactttg ttcgtcatcg atgcgatgct gggtcaggat
     1741 gcggtgaaca ctgctcaggc atttaatgaa gccctgccgc tgaccggtgt cgtattgacc
     1801 aagatggacg gcgattcgcg cggcggcgca gctttgtccg tgcgccacgt aaccggcaag
     1861 ccgattaaat ttatcggtgt cggcgaaaaa atcaacggcc tcgaaccttt ccaccccgac
     1921 cgtcttgcca gccgtatcct cggcatgggc gacgtattga ccctgattga agacgtccaa
     1981 aaaggtattg atgaagaagc cgccgccaaa atggcgaaaa agctacacaa aggcaaaggc
     2041 ttcgacctca acgactttaa agaacaaatc cagcaaatgc gcaatatggg cggtttggaa
     2101 aacctgatgt cgaaaatgcc gggcgaactg ggtcaaatct cgaaacaaat ccccgaaggc
     2161 acggctgaaa aagcgatggg caaagtggaa gccatcatca actcgatgac tccaaaagaa
     2221 cgcgccaacc ctgccctgct taaagccagc cgcaaacgcc gtatcgcagt gggcgcgggc
     2281 acgacggtac aggaagtgaa caaattgctc aaacagtttg aacaaatgca acagatgatg
     2341 aagatgttca gcggcaacgg cttgggcaaa ctgatgcgta tggcgaaagg aatgaggggg
     2401 ataaaaggga tgttcccggg tttgtaagcc gatttaacag aaaacgccgt ctgaaaagtt
     2461 tcagacggcg tttttgtttt atattctgat ttatagtgga ttaacaaaaa tcaggacaag
     2521 gcggcga
//
LOCUS       DAWGJV010000194         2497 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWGJV010000194 DAWGJV010000000
VERSION     DAWGJV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.194"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WHQ94_001952"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WHQ94_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ2129179.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WHQ94_001953"
     CDS             1064..2203
                     /locus_tag="WHQ94_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2129180.1"
                     /translation="MKKTLMAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            2336..>2497
                     /locus_tag="WHQ94_001954"
     CDS             2336..>2497
                     /locus_tag="WHQ94_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2129181.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIESILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          675 a          720 c          623 g          479 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgcctttt ccggtgggca aaaaatcggc
       61 cctcggaaaa cgctgtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt
      121 tgccgcgccg atactttacg ccacactcat cccgacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt cgccgtcgct tccgcacagg
      241 cgggcgcggt agacgcgctt aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cacaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgaccggg ccttttcaaa tgggaataca ccaaacctta caggcaaacc atcgtcggcg
      421 acggtcaaac cgtttggctc tacgatgttg atctggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggct gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggcccggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactgat
     1081 ggcggcggca atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaagccccc tccgccaagc ccgaagccga acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa aaagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctatgac ctgactgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggcgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatacgcc gtcccctatt tctggggcat
     1501 caacaccttg gcaatcaaca cgcggcaggt gcaaaaggca ttgggtacgg acaagctgcc
     1561 cgaaaacgaa tgggatttgg tgttcaaacc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagtg caatcgaaca gattcctttg gcgttgcact atttgggcaa
     1681 agaccccaac agtgagaatc ccgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccggggcgac gtgaaacgct tcagttcttc cggctatatc gatgatatgg cggcgggcaa
     1801 cctgtgtgcc gccatcggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gactccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgtcgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgttacct acgcgcccgc
     2041 cagccgtccc gcgcgcgagc tgatggatga aaaatacacc tccgacgcat cgattttccc
     2101 gaacaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgtcaa
     2161 actgggcgtg aagctgtggc aagggctcaa agcgggcaaa taaccggaat ccctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg gcgtttcccc
     2281 tcctgccggt tatgattggg ttaagattaa aatgatttag taaaatgaga aagatatgga
     2341 tttaagtatc gtagttccta tttataatgt cgaaagttat ttggaagcgt gtttaagttc
     2401 catagaatct atattaagta atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa
     2461 agacggaagt gaagatatat gttataaata tatagat
//
LOCUS       DAWGJV010000195         2469 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWGJV010000195 DAWGJV010000000
VERSION     DAWGJV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.195"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..372
                     /locus_tag="WHQ94_001955"
     CDS             <1..372
                     /locus_tag="WHQ94_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ2129182.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQTFLNAAELMQWSKETEWLGL
                     NVIAHRNFGKQHAQVEFEAYFQDGGNRAAHHELSAFVKIAEQWYFIDSTVPLPTMKQP
                     CICGSEKKFKACCGKYLKPVA"
     gene            complement(591..1385)
                     /locus_tag="WHQ94_001956"
     CDS             complement(591..1385)
                     /locus_tag="WHQ94_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ2129183.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWAEYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTVRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1429..1533
                     /locus_tag="WHQ94_001957"
                     /pseudo
     CDS             1429..1533
                     /locus_tag="WHQ94_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1594..2319
                     /locus_tag="WHQ94_001958"
     CDS             1594..2319
                     /locus_tag="WHQ94_001958"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ2129184.1"
                     /translation="MPRFHLPENLSVGQTVNLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHKDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          666 a          765 c          555 g          483 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactactgtt
       61 ccggcgcaac aaacattcct caatgccgcc gaactgatgc agtggagcaa agaaaccgaa
      121 tggctgggct tgaatgtcat cgcacaccgc aacttcggca aacagcacgc ccaagtcgaa
      181 tttgaagcct acttccaaga cggcggaaac cgagcggcgc atcacgagct gtcggcattc
      241 gtgaaaatag cggagcaatg gtatttcatc gattcgactg ttccgctgcc taccatgaaa
      301 cagccctgca tttgcggttc tgaaaagaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat ttttaacgcg caaaaaaaaa
      421 ccattcatac caagggtaag tatgaatggt caatacattg cgggaaaacg tcttacttgc
      481 tgcactgccg aaaagggaga aacggcagcg gtaatcagcg gaaatgattg tacccgaatt
      541 aatgttaaga aacgttaatc gcgaaaatat attaacaaac ctgttgaaac ctattggttt
      601 tcccgtatcc acccgaccca gcgttcaaac agcttcggtt cgagcgcggc aacgaccgag
      661 cgtttgaaca cgtgttcacc actccaaaac ccgtcgcctt ccaaagtcgt cagcctgccg
      721 cccgcctcct cgaaaatcaa cgcacccgca gcatagtccc acagcttctg cccgccgtgg
      781 acataaacat cataacgccc gcacgccaga taacaccagt ccagcgtgct gctgcccata
      841 ctccgcaccg taccgaaagg tgcgagcgta ctcatgcggc tggaaagttt gcccgaacgc
      901 agatatttga tttccacgcc cgcgattgcc tcattgagtt ttttatccac gaggcgcagg
      961 ggcaggcgcg tcccgtttaa aaacgccccc tgcccgcgtt cggcgtaaaa acattcgccg
     1021 ctgaccgggt tgtagattac gcccaattcg gcgcgcccgt tgcggacaaa cgccaccgac
     1081 acggcaaaat gcggcagccc gttgacaaaa ttgttcgtcc cgtctatcgg atcgacaatc
     1141 cacagcccct tttctcccga atattccgcc cacaaagccg actgctcctg ccgcgacatt
     1201 tcctcgccca acatcgggca atcgatcaga agcggcaacg cggcggcaaa agccgtctgc
     1261 gcggcaatgt ccgcctcgct caacatcgaa ccgtcttcct tgcggtgaga cggcgtattc
     1321 aaaaaacgcg gcataatttc ggtttgcgcg atatggcgca cgactttctg caaacggtgt
     1381 aacacttcct actgtcctca tattttgaac ttgcggcacg cgaacgtata tagtggatta
     1441 aatttaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggcgag
     1501 acaacgctgt actggtttaa atttaatcca ctataatgtc cgtttccatc acgccgctgc
     1561 gacagattat aaccgtccga accgccaaaa actatgcccc gattccacct gcccgaaaac
     1621 ctttccgtcg gacaaaccgt caacctgccc gacaacatcg tccgccacct caacgtcctg
     1681 cgcgtccgcc ccaacgaaaa catcaccctc ttcgacggca aaggcaaggc acacgccgca
     1741 cggctgaccg ttttggaaaa acgccgcgcc gaagccgaaa tcctgcacaa agacacaacc
     1801 gacaacgagt ccccgctcaa catcacactc atccaatcca tatcttccgg cgaacgcatg
     1861 gactttaccc tgcaaaaaag cgtcgaactc ggcgtaaccg ccatacagcc cgtcatcagc
     1921 gaacgctgca tcgtccgcct cgacggagaa cgcgccgcca aacgcctcgc acgctggcag
     1981 gaaatcgtca tctccgcgtg cgaacaaagc ggcaggaaca ccgttccccc cgtgctgccc
     2041 atcatcggct atcgtgaagc actcgacaaa atgccgtctg aaagcaccaa gctgattatg
     2101 agcatcaacc gcgcccgcaa actcggcgac atacgccaac cgtccggcgc aatcgtcttt
     2161 atggtcgggc cagaaggcgg ctggacagaa caggaagaac aacaggcatt tgaagctggc
     2221 tttcaggcgg ttacactcgg caaacggatt ttacgcacag aaaccgcccc actcgccgcc
     2281 ctcgccgcca tgcagacgct ttggggcgat ttcgcataaa cagaaatgcc gtctgaaacc
     2341 cgttcagacg gcattttgca gccgattaag atagtaggtt caaataagat ttcccccgcc
     2401 gtcattcccg cgaaagcaag aatctagaaa cgaaaaacta cagggattta tccgaaacaa
     2461 caaaccctc
//
LOCUS       DAWGJV010000196         2458 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWGJV010000196 DAWGJV010000000
VERSION     DAWGJV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.196"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(16..771)
                     /locus_tag="WHQ94_001959"
     CDS             complement(16..771)
                     /locus_tag="WHQ94_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ2129185.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPKWQKGKVSSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNADAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(952..2108)
                     /gene="nirK"
                     /locus_tag="WHQ94_001960"
                     /pseudo
     CDS             complement(952..2108)
                     /gene="nirK"
                     /locus_tag="WHQ94_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
BASE COUNT          569 a          547 c          680 g          662 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacgtattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaatgcg gcatagttgg acgagtcgct
      121 cgatccgata gacgcgccac tgcaaaacat ttgcgcatcg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagaccg tgcatatcgt aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatagt cggtaggcgt ttgccttgtg cagcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcaggct ctgcctgttt
      541 ggaactgacc ttgccctttt gccatttggg gtgactgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gtaaacttca tatcatgcct
      781 cctgacaggc agttaaaatt gatttttcag acggcatttt aaacaaaaag ccgcgaatgc
      841 cgtctgaaga atcaagcttc caaagtaaaa aggttgatga ttaaaaaacc actgccgtgc
      901 gtaattaagt acggcggcag tggttcgttt gcctaaaaat gggtgttcaa tttagtaatc
      961 gctttttgca ggagcagagg ctgcaggagc agaagctgcg ggagcggaag cagcaggagc
     1021 tgcaccgtta ccggcgtaag cggtatcact caatttttga gtcatgattt cagggttttc
     1081 tgcaccctct actttcaatt gacccagtgc gcctttgttg aatgcgcgga agatagagtg
     1141 gtcaaccaaa gtgtagctgc ccgggatgtc gactttgaat tcgacgatgg cagagccgcc
     1201 ggcaggaacg atggtgcttt gtacgttttc gttaatcagc ttggcgcctt caacataaac
     1261 tttgtcgaag atttcgccga taacgtggaa ggaagacact aggttcggac cgccgttacc
     1321 gacgtacata cgcacggttt cgcctgcttt ggctttcagc gcgttatcgc cggcgataga
     1381 acctacatga ccgttgaata cgacgtattc aggctgttcg gcaacggctt tgtccatatc
     1441 gaacggcagg ccttgcgcgc cttttttgcc tttggtgtag aagtcgcctt ggatgatgta
     1501 gaactcttta tccactttcg gcagaccttc tttaggctcg accaaaatca gaccgtacat
     1561 accgttggcg atgtgcatac cgaccggcgc gacggtgcag tggtagatgt acagacccgg
     1621 ttgcagggct ttgaagctga atgtggaagt gcgacccggg gcggtaaagg ttgcggctgc
     1681 accgccgccc tgaccggttg cggcgtggaa gtcgacgtta tggggaacgg tagaagacgg
     1741 gttgttggaa aattccactt caaccgtatc gctttcgcgt acgcggatca tgcggcccgg
     1801 aacgtcgccg tcaaatgtcc agtagcggta ttccacaccg tcttccatgg tcatggtttt
     1861 tcgacagttt ccatttttac gcggactttg gcaggatagt cgcggtcgat tgcgggaggt
     1921 acttcaggag cgtgggtggt aactgcatcg ataacgggca gttcgcctgt cggcgtttcg
     1981 gcggcggttt gtgcggcgga acttgcggct tctgcggaag cggcaggggc ttgcgcggca
     2041 ggttcgcctc cgcaggcggc taatgcgaat aaggaagcaa tcattgcagc taaggcttgg
     2101 cgtttcataa tgttttcctt ttgtaagaaa agtagaggaa ttggttaaga acttggtgtg
     2161 attatgcgtt tgccccattg ggggcatttt gatttaagtc aaataattca taaaatatga
     2221 acttaaaatg atacttctaa tactttatag ctactaaaaa ttacaatgaa taggctgatt
     2281 tgcttaaggg cttcggcaaa tcaggcaaag gcagggtagt tgccgactaa agaataatga
     2341 tagttattat catatatgta tttgttttat atgtgagttt ctttgaacaa atgtttattt
     2401 gtgcggtaaa ccgtgctaca atctttaaca ttcatattct gtgaatttta aacagctg
//
LOCUS       DAWGJV010000197         2434 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWGJV010000197 DAWGJV010000000
VERSION     DAWGJV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.197"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..979
                     /gene="rfbB"
                     /locus_tag="WHQ94_001961"
     CDS             <1..979
                     /gene="rfbB"
                     /locus_tag="WHQ94_001961"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ2129186.1"
                     /translation="NLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHR
                     PDAVMHLAAESHVDRSIDSAGEFIQTNIVGTFNLLEAARAYWHDMPPEKKAAFRFHHI
                     STDEVYGDLHGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNN
                     YGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGET
                     YNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVRYAIDTA
                     KISRELGWKPQETFESGLHKTVQWYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            1042..1908
                     /gene="rfbA"
                     /locus_tag="WHQ94_001962"
     CDS             1042..1908
                     /gene="rfbA"
                     /locus_tag="WHQ94_001962"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ2129187.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1962..>2434
                     /gene="rfbC"
                     /locus_tag="WHQ94_001963"
     CDS             1962..>2434
                     /gene="rfbC"
                     /locus_tag="WHQ94_001963"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ2129188.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPEAEHSLIWNDPTVGIDWPL"
BASE COUNT          630 a          682 c          598 g          524 t
ORIGIN      
        1 caacctcgac aaactgacct acgcgggcaa tctcgaatcg ctgaccgaca ttgccgataa
       61 tccccgctac gcttttgagc aagtggatat ctgcgaccgc gccgaactcg accgcgtatt
      121 cgcgcaacac cggccagatg ccgtgatgca cttggcggcg gaaagccatg tcgaccgctc
      181 tatcgattcg gcaggcgagt ttatccaaac caatatcgtc ggcacattca atctgctgga
      241 agctgcgcgc gcttattggc acgatatgcc gcctgaaaaa aaagcggctt tccgttttca
      301 tcatatttcg actgatgaag tgtacggcga tttgcacggc acggatgatt tgtttaccga
      361 aaccgcgccc tacgcgccgt ccagccccta ctctgcctct aaagcgtcca gcgaccacct
      421 cgtccgcgcg tggttgcgta cttacggctt gccgaccatt gtaaccaact gctccaacaa
      481 ctacggtcct taccattttc cggaaaaact cattcctttg atgattctga acgcgcttga
      541 cggcaaaccg ctgcctgtgt acggcgacgg tatgcaaatc cgcgactggc tgtttgtcga
      601 agaccacgcg cgcgcactgt atcaggttgt taccgaaggt gttgtcggcg aaacctacaa
      661 tatcggcggc cacaatgaaa aagccaatat tgaagtcgtc aaaaccatct gcgccctgct
      721 ggaagaactc gctcccgaaa aaccggccgg tgtggcgcgt tatgaagatt tgattacttt
      781 cgtacaagac cgccccggcc atgacgtacg ctacgccatt gatacagcca aaatcagtag
      841 ggaactaggc tggaaaccgc aggaaacctt cgagtccggc ctccacaaaa ccgtgcaatg
      901 gtatctggac aacaaaacct ggtggcaaaa cgtattgaac ggcagctatc gtttggaacg
      961 cttaggtaca ggcaaatagt tttcagacgg catcccgacg caatgccgtc tgaaaaccca
     1021 tcgcaaagga agaaagaaaa gatgaaaggc atcatactgg caggcggcag cggcacgcgc
     1081 ctctacccca tcacgcgcgg cgtatccaaa cagctcctgc ccgtgtacga caaaccgatg
     1141 atttattacc ccttgtcggt tttgatgctg gcaggaatcc gcgatatttt ggtgattacc
     1201 gcgcctgaag acaacgcctc tttcaaacgc ctgcttggcg acggcagcga tttcggcatt
     1261 tccatcagtt atgccgtgca acccagtccg gacggcttgg cacaggcatt tatcatcggc
     1321 gaagaattta tcggcaacga caatgtttgc ttggttttgg gcgacaatat tttttacggt
     1381 cagtcgttta cgcaaacatt gaaacaggcg gcagcgcaaa cgcacggcgc aaccgtgttt
     1441 gcttatcagg tcaaaaaccc cgaacgtttc ggcgtggttg aatttaacga aaacttccgc
     1501 gccgtttcca tcgaagaaaa accgcaacgg cccaaatccg attgggcggt aaccggcttg
     1561 tacttctacg acaaccgtgc cgtcgagttc gccaaacaaa tcaaaccgtc cgcacgcggc
     1621 gaattggaaa ttaccgacct caaccggatg tatttggaag acggctcgct ctccgttcaa
     1681 atattgggac gcggtttcgc gtggctggac accggtacgc acgaaagcct gcacgaggcg
     1741 gcttcgttcg tccaaaccgt gcaaaacatc caaaacctgc acatcgcctg cctggaagaa
     1801 atcgcttggc gcaacggttg gctttccgat gaaaaactgg aagaattggc gcgcccgatg
     1861 gcgaaaaacc aatacggcca atatttgctg cgcctgttga aaaaataatg tttgaggccg
     1921 tctgaaactt ttcagacggc ctttagatga aagataaaaa gatgaacatc attgataccg
     1981 ccattcctga cgtaaaactg cttgagcccc aagtcttcgg cgacgcgcgc ggctttttta
     2041 tggaaacctt ccgcgacgag tggtttaaaa cccaagtctg cgaacgcacc ttcgtgcagg
     2101 aaaaccactc caaatccggc aaaggcgtat tgcgcggcct gcactaccaa accgaaaaca
     2161 cacaaggcaa actcgtacgc gtagttgtcg gcgaagtgtt cgacgtggcc gtcgatatgc
     2221 gtaaagactc ccccactttc ggcaaatggg taggcgagat tctgtccgca gaaaacaaac
     2281 gccaactgtg ggtacccgaa ggtttcgcac acggcttcta tgtactgagc gatgaagccg
     2341 agttcgtcta taaatgcaca gactattaca accctgaagc cgaacactca ctaatttgga
     2401 atgatccgac cgtcggcatc gactggccgc ttca
//
LOCUS       DAWGJV010000198         2433 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWGJV010000198 DAWGJV010000000
VERSION     DAWGJV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.198"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            35..1390
                     /locus_tag="WHQ94_001964"
     CDS             35..1390
                     /locus_tag="WHQ94_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ2129189.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYYKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WHQ94_001965"
     CDS             1394..2404
                     /locus_tag="WHQ94_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ2129190.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAIAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          438 a          663 c          739 g          593 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactcttg aaaaccgcgc ctaaagcgtt ttggacggtt actttggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaatactat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggagtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc attgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gcc
//
LOCUS       DAWGJV010000199         2425 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWGJV010000199 DAWGJV010000000
VERSION     DAWGJV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.199"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(119..1570)
                     /locus_tag="WHQ94_001966"
     CDS             complement(119..1570)
                     /locus_tag="WHQ94_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ2129191.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1593..2213)
                     /locus_tag="WHQ94_001967"
     CDS             complement(1593..2213)
                     /locus_tag="WHQ94_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ2129192.1"
                     /translation="MNSTASNTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          757 a          579 c          571 g          518 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaatg acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacgatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcaatccaa cgacgaaaat gcggcgtatg ccagacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     1081 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     1141 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     1201 cggatttgtt gccatccgta cccacatggt gtacgagcgg gaaaatcaac cctctgacga
     1261 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     1321 accacgcagc acccaaaatc aaaaagtcgg caatacccgc ccacaagaag cactgcccga
     1381 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgacgg
     1441 cgataccggt cagaaaacag gcaagagtaa atgaaaatgc ctgaggcacg gactgtgctg
     1501 cgaacgaaaa catccttacc cacaagactt ctatacccaa gctcaataag ccgctaagga
     1561 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt cactatatcc atcctttctg
     1621 taacgcagcc atactgaagc agcaatcaga aggttaaagc aggctgtcag cgcaatggtt
     1681 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     1741 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     1801 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     1861 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     1921 ccggaaatca gacccctgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     1981 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccaccgaa atacgcacct
     2041 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaactcaa atcgatacct
     2101 atgtgactga atagaagcct ctgccagctg acctggtaaa tcagggcgca gaatccagag
     2161 gcgaaaaaca ccagcgacaa tcctttcagg gtgttacttg cggtcgaatt catattttct
     2221 attttcaaat aggcggaact ataaatgaaa tgtatcaatg tgtaaacatt tgtattaacc
     2281 gtaggaaatc accgtattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     2341 acggcgtatc actgttcaaa ctgctgtgcg gcttcacggt gttataaaaa taatggggct
     2401 gtcctggaca actaagataa actcg
//
LOCUS       DAWGJV010000200         2374 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWGJV010000200 DAWGJV010000000
VERSION     DAWGJV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.200"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            79..2292
                     /locus_tag="WHQ94_001968"
     CDS             79..2292
                     /locus_tag="WHQ94_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ2129193.1"
                     /translation="MTAISPIQDTQSATPQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QENYPADAATPYGEPLSDHFLGAAQIVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          612 a          764 c          574 g          424 t
ORIGIN      
        1 attttagtaa cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc
       61 ggaacggaag accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgacc
      121 ccgcaagaat tgcgcgaatg gttcgacagc tactgcgccg cgctgccgga caacgataaa
      181 aacctcatcg gtaccgcatg gtcgctggcg caggaaaact atcccgccga tgccgccacg
      241 ccgtatggcg agccgttgtc cgaccacttc ctcggtgcgg cgcaaatagt ccatgaactc
      301 gacctgctgc ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc
      361 gactggaacc tattggtttc cgaacgctgc aacagtaccg tcgccgagct ggtcaaaggt
      421 gtggacgaag tgcagaaact cacccacttc gcccgggtgg acagcctcgc cacgccagaa
      481 gaacgcgccc agcaggcaga aactatgcgg aaaatgctgc tggcgatggt taccgacatc
      541 cgcgtcgtgt taatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgcc
      601 cccgacagcc ccgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc
      661 gccaaccgtt tgggcgtgtg gcagctcaaa tggcagctcg aagatttggg cttccgccat
      721 caagagcccg aaaaataccg cgaaattgcc ctgcttttgg acgaaaaacg taccgaacgc
      781 ctcgaataca tcgaaaactt ccttaatatc ctgcgtacgg aactcaaaaa atacaatatc
      841 cactttgaag tcgccggccg tccgaaacac atctactcca tttacaaaaa aatggtgaag
      901 aaaaaactca gcttcgacgg gttgttcgac atccgcgccg tgcggattct ggttgatacc
      961 gtccccgagt gttacaccac actgggcatt gtccacagcc tctggcagcc cattcccggc
     1021 gagttcgacg actacatcgc caacccgaaa ggcaacggct ataaaagttt gcacaccgtc
     1081 atcgtcggcc cggaagacaa aggcgtggaa gtgcaaatcc gcaccttcga tatgcaccaa
     1141 ttcaacgaat tcggtgtcgc cgcgcactgg cgttacaaag agggcggcaa aggcgattcc
     1201 gcctacgaac aaaaaatcgc ctggttacgc caacttttgg actggcgcga aaacatggcg
     1261 gaaagcggca aggaagacct cgccgccgcc ttcaaaaccg agcttttcaa cgacacgatt
     1321 tatgttttga ccccgcacgg caaagtcctc tccctgccca caggcgcgac ccccatcgac
     1381 ttcgcctacg ccctgcacag cagcatcggc gaccgttgcc gcggtgcgaa agtcgaaggg
     1441 cagattgtgc cgctgtccac cccgctcgaa aacggacagc gtgtcgaaat cattaccgcc
     1501 aaagaagggc atccttccgt caactggctt tacgaaggct gggtcaaatc caacaaggca
     1561 atcggcaaaa tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc
     1621 cgcgtccaac tcgacaaaca gcttgccaaa ctcacgccca aacccaacct gcaagagctt
     1681 gccgaaaatc tcggctacaa aaagccagaa gacctctaca ccgccgtcgg acaaggcgaa
     1741 atttccaacc gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccgtaccc
     1801 gtcagcgaaa ccaccatcgt caaacagtcc aaaatcaaaa aaggcggcaa aaacggcgtg
     1861 ctcatcgacg gcgaagacgg tctgatgacc acgcttgcca aatgctgcaa acccgcgccg
     1921 cccgacgaca ttgtcggctt cgttacccgc gatcgcggca tttcggtaca ccgcaaaacc
     1981 tgcccctctt tccgacacct cgccgaacac gcgcccgaaa aagtactgga cgcaagttgg
     2041 gcggcgttgc aggaaggaca agtgtttgcc gtcgatatcg aaatccgcgc ccaagaccgc
     2101 tccgggcttt tgcgcgacgt atccgacgcg ctcgcccgcc acaaactcaa cgttaccgcc
     2161 gtgcaaaccc agtcccgcga cttggaagcc agtatgcggt tcacgctcga agtcaaacag
     2221 gtcaacgacc tcccgcgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt
     2281 acccggcttt aaatacaaaa atgccgtctg aaagccgaat aacgcttcag acggcatttt
     2341 gattgccggg gtttgctatt ttttgttgca tagt
//
LOCUS       DAWGJV010000201         2326 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWGJV010000201 DAWGJV010000000
VERSION     DAWGJV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.201"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            299..733
                     /locus_tag="WHQ94_001969"
     CDS             299..733
                     /locus_tag="WHQ94_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129194.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1009..1728
                     /locus_tag="WHQ94_001970"
     CDS             1009..1728
                     /locus_tag="WHQ94_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129195.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            1715..1888
                     /locus_tag="WHQ94_001971"
                     /pseudo
     CDS             1715..1888
                     /locus_tag="WHQ94_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            1946..>2326
                     /locus_tag="WHQ94_001972"
     CDS             1946..>2326
                     /locus_tag="WHQ94_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2129196.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYH"
BASE COUNT          798 a          284 c          420 g          824 t
ORIGIN      
        1 caaacaaaaa agagaaattt taatttcgct aaatcgcata aagattaatc aagagtatca
       61 ttaaatgata tgagtgagca tctataatgc caagaagagt tgttaagaca taacgattat
      121 tgaaatagat tgtaaaatag atacttagat agtctgaaaa acggatttgt gaaacttttt
      181 attacgcgcc atcatttgaa aatgaaactt aaaaaacact tatcataata aatattttct
      241 ttacgttgtt tgctaataaa ctcagtgcaa tatcagcgca atattttatg gaaattttat
      301 ggataacaaa aaagaattta ttaataattt aacaaatagg tatatgtgga tctatccatt
      361 ggtcttaaat attctatttc taccttttta ccagtcctac caatcttttt ttattgcgct
      421 tggttgtttg tttgcactgg ttagaaaaat gcaaagctta gattttaaat tacaaaatca
      481 tattgtattg ttaaatataa aaagtgcttg ggcagataaa aaagtatttt tgattaggat
      541 agtagtgtca tggttggcag taatggaaat atggatgtgt tttatttcgg aatcatcaac
      601 gtgggtatgc ggtgcttttt gtttaaatag tgaaatattg gaaaaaattt ttcgtggctt
      661 tggttattct ggtagtttat attttttatt tatattgatg attgatctca ataagttaag
      721 agagagtatt tgaatttcat tttttgtttg acttaaactc aaggagagta acaatgattg
      781 gtagtggtga tactaaacaa tgcaaaaaat tttctgcgtg tgatggaaaa taccacgtct
      841 acgatcccct cgccctagac ttggacggcg acggcataga aacagtcacc gccaaaggct
      901 tttcaggcag cctgaagact gagagagtga atacgatgag tatacactct atgccactaa
      961 attgatattc actaaatcat accagctata ttttatttaa tgagacatat gaaaaataaa
     1021 aattatttac tagtatttat agttttacat atagccttga tagtaattaa tatagtgttt
     1081 ggttattttg tttttctatt tgattttttt gcgtttttgt tttttgcaaa cgtctttctt
     1141 gctgtaaatt tattattttt agaaaaaaac ataaaaaaca aattattgtt tttattgccg
     1201 atttctatta ttatatggat ggtaattcat attagtatga taaatataaa attttataaa
     1261 tttgagcatc aaataaagga acaaaatata tcctcgatta ctggggtgat aaaaccacat
     1321 gatagttata attatgttta tgactcaaat ggatatgcta aattaaaaga taatcataga
     1381 tatggtaggg taattagaga aacaccttat attgatgtag ttgcatctga tgttaaaaat
     1441 aaatccataa gattaagctt ggtttgtggt attcattcat atgctccatg tgccaatttt
     1501 ataaaatttg caaaaaaacc tgttaaaatt tatttttata atcaacctca aggagatttt
     1561 atagataatg taatatttga aattaatgat ggaaacaaaa gtttgtactt gttagataag
     1621 tataaaacat tttttcttat tgaaaacagt gtttgtatcg tattaattat tttatattta
     1681 aaatttaatt tgcttttata taggacttac ttcaatgagt tggaatagtt ttggtaattt
     1741 tatgagcgca cgctcatccg cgttagcaga atttggaaat atggttgcta atttagtttc
     1801 tgcaaaaaat gagaaagata tctcgaaacg taatgaatat tacaaacaag ctggttatag
     1861 tgcattatta gcatttggta atttggctag taatattgca ccaggtagta cgtcatcgca
     1921 tattgtaaac ggaacaaatg cctctgtgat tgcaagccgt ctctctggaa atatatcttc
     1981 agctattcag gagcataaag atggtaaagt taatatcaac cgttttcaaa atattttagc
     2041 ggatttatat tcattgggag ggttaggaag tacattaata gagaagaatg gaaatatgca
     2101 gagttggggg attccattag caattgctgg agatataatt gcagcaacgg ctattgccac
     2161 aggagatact ggtacgatat ctacagagga attttataat tttgacaact ggaaaggttt
     2221 tgggtatgag ctatttgaag actggtctcg ttgggtatac gactggctgc ccgacggctg
     2281 gaatctgtgg aaagaattgg acagaaaccg ttcaggccaa taccac
//
LOCUS       DAWGJV010000202         2291 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWGJV010000202 DAWGJV010000000
VERSION     DAWGJV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.202"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(14..601)
                     /locus_tag="WHQ94_001973"
     CDS             complement(14..601)
                     /locus_tag="WHQ94_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ2129197.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            764..2170
                     /gene="dnaB"
                     /locus_tag="WHQ94_001974"
     CDS             764..2170
                     /gene="dnaB"
                     /locus_tag="WHQ94_001974"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ2129198.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          558 a          591 c          613 g          529 t
ORIGIN      
        1 ggattaacaa aaatcaggac aaggcggcga agcgtttggc gacttcgtcc cagttgacga
       61 tttcccagaa acctttcagg tagttggggc ggctgttgcg gtagtcgatg tagtaggcgt
      121 gttcccacac gtcgcaggtc agcagcggcg tgttttcagt ggtcagcggc gtagcggcgt
      181 tggaagtaga aaccaaatcc aatccgccgg caggggtttt taccagccac gcccaaccgg
      241 agccgaaagt accggccgcg caggcattga acgcttcttg gaatttctcg aagctgcccc
      301 atttcgcgtc gatggcggcg gccagttcgc cggcaggttt gccttgacct ttggacgtga
      361 aacccagcca gtagaaggtg tggttccaag tttgtgccgc gttgttgaac acgccgcccg
      421 aagatttttt cacaatctct tccaaaggca ggttttcaaa ttcggtgcct ttgatttgat
      481 tgttcaggtt ggtgatgtag gtttgatggt gtttgccgta gtggaattcc aaagtctctt
      541 tgctcagatg cggggacaat gcgtccagtt cataaggcag ttgcggcagc ttatgttcca
      601 ttttgtgctc ctaatattgt tttaaatgtt gtattttggc agtgttgctg caaatagctc
      661 agcagcgcac atattttacc tgttttgagg gatggaaacc aattaaacct gctttacgct
      721 ataatagaag attgcaattt cggcacgaca gataggatgt accatgaacg attacacagc
      781 catgccgtct gaagaccgtg agatcggcgc attgtcgctg cctccacatt cgatggaggc
      841 ggaacaatcc gttttgggcg ggttgatgct ggaaaatccg gcatgggaca ggattgccga
      901 tgtggtttcg ggagaggatt tctaccggca tgaacaccgc ctgattttcc gatccattgc
      961 taaattaatc aatgagagcc gtcccgcaga tgtgattacg gttcaggaag atttgcagcg
     1021 gaacgaagaa ttggaagcgg caggcggatt cgaatacctg attacgctgg cgcaaaacac
     1081 cccgtctgcc gccaacatcc gccgctacgc cgaaatcgtg cgcgagcgtt ccattatgcg
     1141 ccaactcgcc gaagtgggga cggaaatcgc ccgcagcgcc tacaatccgc aaggcaggga
     1201 cgcggggcag cttttagacg aggcggaaaa caaagtattc caaatcgccg aaagcaccgc
     1261 caaatccaag cagggctttt tggagatgcc cgatttgctg aaagaagtcg tacagcgcat
     1321 cgatatgctc tactcgcgcg acaatcccga tgaagttacc ggcgtgccga cagggttcat
     1381 cgacctcgac aaaaaaacct cgggtctgca acccggcgac ctgattatcg ttgccggtcg
     1441 tccgtctatg ggtaagaccg ccttttccat caatatcgcc gaacacgttg ccgtagaagg
     1501 caagctgccg gttgccgtct tctcaatgga gatgggcggt gcgcaactgg tcatgcgtat
     1561 gctcggctcg gtcggacgct tggatcaaag cgtcctgaaa accggcaggc tcgaagacga
     1621 acactggggt cgtctgaatg aagcagtcgt caaactctcc gacgcgcccg tgtacatcga
     1681 cgagaccccg ggtttgactg cgctcgaact gcgcgcccgc gcccgccgtc tcgcccgtca
     1741 atttaacaat aagctgggat tgatcgtcat cgactacctg caactgatgg cgggatccgg
     1801 ccgttccgac aaccgagctt cagagctggg agagatttca cgttcgctca aagccctggc
     1861 aaaagagttg caagtcccca tcatcgccct gtcgcaattg agccgtaccg tcgaatcgcg
     1921 taccgacaaa cgcccgatga tgtccgacct ccgcgaatcc ggtgctatcg agcaagatgc
     1981 cgacctgatt atgttcatgt atcgcgacga atactacaac caagactcac ccatgaaagg
     2041 ccttgccgaa tgtatcatcg gcaaacaccg caacggtccc gtcggtaaaa tcttcctcac
     2101 atggacgggg caattcacca aattcgacaa tgctgcctat attcccgagg aggcaaagat
     2161 agaggattaa atggctatat aaaaatttat taggcgaaat caggcaaaat cgtttaaaat
     2221 catgcttaga gattgcccta aaaaataaaa cgcggtcttg aggcattttt gcattcagcc
     2281 cgcatataat t
//
LOCUS       DAWGJV010000203         2291 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWGJV010000203 DAWGJV010000000
VERSION     DAWGJV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.203"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(29..1438)
                     /gene="dacB"
                     /locus_tag="WHQ94_001975"
     CDS             complement(29..1438)
                     /gene="dacB"
                     /locus_tag="WHQ94_001975"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ2129199.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1603..2169
                     /locus_tag="WHQ94_001976"
     CDS             1603..2169
                     /locus_tag="WHQ94_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ2129200.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          522 a          559 c          664 g          546 t
ORIGIN      
        1 taaatttaat ccactatatt ttcctgtttc aggctcggcg ttctttgcac atcagtttcg
       61 catccagcca gccatcgccg ccggagatga tgttgttggc aacgaagttg tccaagtcgg
      121 gcagcaggga aacggcgcga ccgctgttga tgatgacgac caccaccatc ggtttgtcgc
      181 ccagccagta gcctgccaat gcgcggacat tgttgagcgt gccggttttc aggcgcagaa
      241 gcccaccgct ttgtttgaag cggttgcgta aagttccgtc tgtgccggcg atgggcagcg
      301 tgtcgatgaa atcttgtgca aacgggctga aataagccgt ttccaacatt tgcgccatca
      361 ttctcgccgt taccctttct tttctagaca gacccgaacc gttttccaaa accaaatccg
      421 caacatcgat gcccgacacg gcaagttcgc gccggacggc agacgctgcc tgttcggaaa
      481 cggcgggcag tttgccgtcg ccgccgagtt tgaggaagac ggaacgcgca attagattgt
      541 ccgaacgctt gttcatgtcc gtcaaaattt ccttcatcgg tttggcgtgt gcaacggcaa
      601 gcgtctgcgc gccttccggc gtgtcggcta tgccgatacc gtctgaaatc cgtccgccgc
      661 cgagcagcca gtggttggta aaactttgcc ggatcagttc gtcaagcgcg aacatccgga
      721 caccgacagg cttgcccaaa cagctctcgg gaatattgcc gcgcaatttc agcgtattgt
      781 ccgaaaaaga tgcacgcatc agttttttga tcgaagggca ggcggcttgg gaggcggtaa
      841 ttttcaagtt gttttgggcg aaaatatgcg gcaaaggcgg atcggtgagg atgtcggtac
      901 tgccggcggc attgcgttcg gcgcgcacca taaccatacc ggcagacagc atagtcggat
      961 tggggggggt cataaacggc gaaccgctgt cggcttcaaa atggtcggga ctgccgactt
     1021 cgccccacag gctgtggtcg agcatcaggt gtcccgtgat attgcgtatg ccttgttcgc
     1081 gcaactggcg ttggacggca agcaggtttt cctgattgaa aacggggtcg ccgctgcccg
     1141 cccaatacag gtttccgtca agcgtgccgt cgtttaccgt accgttgctt ttaaactcgg
     1201 tcgcccagcg gtaattgctg ccgaaggttt tgaaggcggc aaacgcggta acgagtttca
     1261 ttgtggaggc ggggttgacg gggacatccg agcggtggtc aatgatgact tttccgctgt
     1321 caagctcttg gacatatacg gcgatttcgt tttgcggaat gcggccggta tcgagcgcgt
     1381 gtgcggcgag ggaggcgaga agcagcagca gggaggccgc tgttttgggg aaattcatag
     1441 gtaaatctgt tacataaaaa aagcaattat aaggcaaagc cggataagtg ggaacgctgt
     1501 ggcggcgggc cggcttgttt gcaggggaaa tcacatatat aataatcgct atcattatga
     1561 aatgattgag acgcacaaac ttaatattca ggaggaatga ttatggctaa gaaaatcagt
     1621 attttggtgg gcagcctgcg ccgtgcttct tttgcgcgca aagtggcatt gaatgcggcg
     1681 gagatgttcc ccgaaggctg gcaggcggaa atcgtcgaaa tcggacatct gccgctttac
     1741 aatttcgatt atgacgaccc tgcggtggaa gatgtgccgc tgcccgaaag ctacacggct
     1801 ttccgcgaaa cgattaaggc ttcggacggc attttgttcg ttacgtccga aaataaccgc
     1861 accattcccg cctgtttgaa aaatgcggtg gacatcggct cgaaaccgaa tgccgacgtg
     1921 gcgtggaaaa acaaaccggc cggtatcatc agccattccg tcggcaagat gggcggttac
     1981 agttcgcaaa aaaacctgcg ccttgcgctg tcttatttcg atatgcccgt aaccggacag
     2041 ccggaagtgt ttttgggcaa ttcgccgacg ctgtttgatg aaaacggcaa attgatcgac
     2101 tcggcaaggg attttgttca gtcatatatt aatcagtttg tcggtttgat tgaaagaaat
     2161 gccaaataaa caaaccaagt cgaaaggccg caaaaccggt tcgggttttg cggctttttt
     2221 atatgggctg ggcagcgtca aggtttgccg tttgccggat aatgcgcggc aaatgaccgg
     2281 cgtgtttata g
//
LOCUS       DAWGJV010000204         2274 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWGJV010000204 DAWGJV010000000
VERSION     DAWGJV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.204"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..2123
                     /locus_tag="WHQ94_001977"
     CDS             <1..2123
                     /locus_tag="WHQ94_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2129201.1"
                     /translation="NKPNPNPKAQALSPALTAPALWFGAGQNGKAEMYSASVSTYPDS
                     SSSRIFLQNLKRKNDPNKPGRYSLADLAKSDIENRQPNFTGRQTIIRLDGGVREIKLN
                     RSHEATGLNGNTNNNTFGIVQEYGFKPQDDEWKKVLLPWTVRASNDDNQFKTINQQLN
                     QQKIQYSQRYRIRENGNNGKRDLGDIVNSPIVAVGEYLATSANDGMVHIFKKGNGDAR
                     NYSLKLSYIPGTMPRKNIENQDSTLAKELRAFAEKGYVGDRYGVDGGFVLREVERDGK
                     TRVFMFGAMGFGGRGAYALDLTKADGSDPTAVSLFDVKNDKNKGNNSAELGYTVGTPQ
                     IGKTHNGKYAAFLASGYATKDVNSNDNTTALYVYDLESSGNLITKIDVPGGKGGLSSP
                     TLVDKDLDGTIDIAYAGDRGGSMYRFDLSSQDPKQWSARAIFSGNKPITSAPAISQLK
                     DKRVVIFGTGSDLSEDDVDNTDEQHIYGIFDDDTATTGSVNFSGTGGGLLEQHLTQEN
                     KTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIRKYKDNGCGAETAI
                     LGINTADGGKLTKKSARPIVPADNTAVAQYSGHKKGTNGKSIPIGCMEKNGGTVCPNG
                     YVYDKPVNVRYLDEKKIDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMN
                     DLDSLDITGPTCGMKRISWREIFY"
BASE COUNT          634 a          598 c          588 g          454 t
ORIGIN      
        1 tcaataaacc gaaccccaat cccaaagccc aagccctttc gccggcactg actgcccccg
       61 cgctgtggtt cggagctggg caaaatggta aggcggagat gtattccgct tcggtttcca
      121 cctaccccga cagttcgagc agccgcatct tccttcaaaa tctgaaaaga aaaaacgacc
      181 ccaacaaacc cggccgctat tccctcgcag acttggctaa gtcggatatt gaaaatcgac
      241 agccgaattt cacagggcgg caaacaatca tccgattgga tggcggcgta cgtgagatca
      301 aactgaatag aagccatgag gccaccggtt taaatggaaa taccaacaac aacactttcg
      361 gcattgttca ggaatatggc ttcaagcctc aggacgatga gtggaaaaaa gtgctgctgc
      421 cttggacggt tcgtgcttcc aatgatgaca atcaatttaa aacaattaac caacaactta
      481 accaacaaaa aatccaatac agccaaagat accgcatccg cgaaaacggc aacaacggca
      541 agcgcgattt gggcgacatc gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta
      601 cttccgccaa cgacgggatg gtgcatatct tcaaaaaagg caacggggac gcgcgcaact
      661 atagtctgaa gctcagctac atccccggca cgatgccgcg caagaatatt gaaaaccaag
      721 actccaccct cgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtg ggcgaccgct
      781 acggcgtgga cggcggcttt gtcttgcgcg aagtcgaacg ggacgggaaa acccgcgtgt
      841 ttatgttcgg cgcgatgggt tttggcggca gaggcgcgta tgccttggat ttgaccaaag
      901 ccgacggcag tgacccgacc gccgtttccc tgtttgatgt caaaaatgac aaaaataaag
      961 gcaataatag tgcagaatta ggctacaccg tcggcacgcc gcaaatcggc aaaacccaca
     1021 acggcaaata cgccgccttc ctcgcctccg gttatgcgac taaagacgtt aacagcaacg
     1081 acaatacaac cgcgctgtat gtatatgatt tggaaagcag cggcaatttg attacaaaaa
     1141 tcgatgtacc cggcggcaag ggcgggcttt cgtcccccac gctggtggat aaagatttgg
     1201 acggcacaat cgatatcgcc tatgccggcg atcgcggcgg cagtatgtac cgctttgatt
     1261 tgagcagtca agatcctaaa caatggtctg cacgcgccat tttcagcggc aacaaaccga
     1321 ttacttccgc gcccgccatt tcccaactga aagacaaacg cgtggttatc ttcggcacgg
     1381 gcagcgattt gagtgaggat gatgttgata atacggatga acaacatatt tacggtatct
     1441 tcgacgacga tacggcgacg acgggttctg taaacttcag cggtacggga ggcgggctgc
     1501 tcgagcaaca ccttactcag gaaaataaaa ccttattcct gaccgattac aagcgatccg
     1561 acggatcggg cagcaaaggg tgggtagtga aattgaagga cggacagcgc gttaccgtca
     1621 aaccgaccgt ggtattgcgt accgcctttg taaccatccg caaatataaa gacaacggct
     1681 gcggcgcgga aaccgccatt ttgggcatca ataccgccga cggcggcaag ctgaccaaga
     1741 aaagcgcgcg cccgattgtg ccggccgaca atacggctgt cgcgcaatat tccggtcata
     1801 agaaaggcac caacggcaaa tccatcccta taggttgtat ggaaaaaaac gggggaaccg
     1861 tctgcccgaa cggatatgtt tacgacaaac cggttaatgt gcgttatctg gatgaaaaga
     1921 aaatagacgg attttcaaca acggcagacg gcgatgcggg cggcagcggt atagaccccg
     1981 ccggcaagcg ttccggcaaa aacaaccgct gcttctccca aaaaggggtg cgcaccctgc
     2041 tgatgaacga tttggacagc ttggacatta ccggcccgac gtgcggtatg aaacgaatca
     2101 gctggcgtga aatcttctac tgatttgcac gcgaaaatgc cgtccgaaag gttttcggac
     2161 ggcatttttt gcgtttttcg ggaggggcgg gttcgtaaaa ggcgggctat agggtaggct
     2221 tcatctcgcc aatctcactg aatccatcaa tttccacaat tcaattaaat accg
//
LOCUS       DAWGJV010000205         2216 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWGJV010000205 DAWGJV010000000
VERSION     DAWGJV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.205"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            136..2016
                     /locus_tag="WHQ94_001978"
     CDS             136..2016
                     /locus_tag="WHQ94_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ2129202.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFTE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMKKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVALAGKTAGN
                     AV"
BASE COUNT          603 a          673 c          519 g          421 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctcccc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgacgc tacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt taccgagttc gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga tatcgacacc gatttattgt gggaagtgtg cggcgaagag
      361 gaatttaccg ctgaagccat agccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcga ccttgattgc gctgtacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcagaag ccttgaaacg cggtgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg cttccccgac cttccgcttc ccgaaatgcc cgacctgccc
      841 aaggctgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttgga caacggcacg aagcgcgtcg gcatccacat cgccgcgccg
      961 tcgcttgcca ttaaacaagg cgacaaaatg aaaaaaaaca tcatggaacg cttgagcacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgagcccaa ccgtgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggctcac ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcacaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcagca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgatttcc aacggcagat ggaagcctac tggagccttg tgtacctgca acagcaaggt
     1801 acaagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtcacgc gcgcgaccgg tattccgttt gacgcgctgc ccaaatcaca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcactcgcag gcaaaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgata
//
LOCUS       DAWGJV010000206         2204 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWGJV010000206 DAWGJV010000000
VERSION     DAWGJV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.206"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WHQ94_001979"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WHQ94_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ2129203.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          558 a          625 c          581 g          440 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgagccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgatacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcagggttga
      601 gcggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggcatcggc gtaaccatca
      961 ctgataaaga taaccccaaa ggcctgccga tgttgagcgt ggacgaaccg acgctgacca
     1021 tggactttat ggttaacacc agcccgttgg cgggcacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gacgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggcttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcctggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggtatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc cgattaagct gacgctggaa ggtgcggtcg
     1801 agtttatcga cgatgatgag ctggtagaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc atttcaaaaa gctagattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc cgccgccatt
     2041 tttccagcgg catatcgccc cgtcgcgttt cggtatcggg ttcggcttcc cggcagcatg
     2101 tcgaacaatg ccgtttgaag gaatacgcct tttgagtcct tacaagctta ggaagaggat
     2161 atagtggatt aacaaaaatc aggacaaggc gacgaagccg caga
//
LOCUS       DAWGJV010000207         2203 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWGJV010000207 DAWGJV010000000
VERSION     DAWGJV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.207"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(383..793)
                     /locus_tag="WHQ94_001980"
     CDS             complement(383..793)
                     /locus_tag="WHQ94_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129204.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1381..1764)
                     /locus_tag="WHQ94_001981"
     CDS             complement(1381..1764)
                     /locus_tag="WHQ94_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129205.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(1730..>2203)
                     /gene="mafB"
                     /locus_tag="WHQ94_001982"
     CDS             complement(1730..>2203)
                     /gene="mafB"
                     /locus_tag="WHQ94_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2129206.1"
                     /translation="AGTVWRGKKVELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGN
                     KPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSP
                     DARFIQVRDSNGNLTGARIDDAHHANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
BASE COUNT          643 a          400 c          363 g          797 t
ORIGIN      
        1 ctctacggat ggggcagagt tacccgtatg attgccgtat ggtcggatac ttgtatccga
       61 caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaat gtcggattcg
      121 agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa tccatgtttc
      181 gccattttcc tttatatata cctaatacag cacgggttac gatttcgttt aagtcataaa
      241 tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa acccaacaaa
      301 aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg aattaggttt
      361 taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgt actgaacaaa
      421 caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa atgtttacat
      481 ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca tgaggatatt
      541 tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt aattttgcac
      601 acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca attccttcat
      661 cccaatcaaa agtaaaatcc gttccttcta tgataaattt agcacaagat attaaatcta
      721 acttaaccca agtggattta tacagactca atgctgatga agttataata ttatctaaat
      781 tataaatttt cataaatcaa cgttccttac ctggtgttat atgcacaggt ctaatcccat
      841 ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg tctgtatagc
      901 caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa ttatgtttat
      961 ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat ttaaataaac
     1021 ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtaaa aagcactgtc
     1081 ctgtaatagg ctcgcggcag caagttgtgc ttccggactt aattttccta aatcattaat
     1141 accttgtaaa gtttgctgcg cagagatgta ggtcggatat ttgtatccga caaaaatatt
     1201 tgacgcatct atcgtttccg aaatgccgct gttggaaaat gtcggattcg agaatctgac
     1261 ctacggctgc tgactatttt cttgtttata taattctaca actgcaaact actatcaaat
     1321 ctttttatct ctggctctac actcagcaag aatattttta ttttacaaaa cataggctag
     1381 ttatttgagt atattgtcaa attctaattt gtttttttga tagatttttt ttataccatt
     1441 aatacattca ttagttgtaa ttctaccaag cgaatgtaat ttatcaatat caaaacaatc
     1501 ggtataaacc ctattcacag ttttatcttg tatttcctta tgaatactta atatattgag
     1561 atagtttaga aattttattt tctcagattg aggtaactca tcaaaaccaa gccaataata
     1621 aaaattgtta tatgatttta aaaaatcaac aatttttatt tttcctaaaa ccaaatcttc
     1681 aactaactgg gttgcataaa taatatcatt tatgatttta acaggtaaat tatttgatgg
     1741 gtaaccatgg ggttccgtca tcatttgtaa ctcctggtac atgtgcgtgt cctgtaagtg
     1801 cgcgtgggtc ttgatgggta tttgcatgat gagcatcatc tattctggct ccagtcaagt
     1861 ttccattact atctcggact tggataaaac gagcatcagg cgaacctgtt atataaccgc
     1921 ctattggtgt tggtattgca tttcctttgc tgtctacata aattgttcct gtcggagaaa
     1981 tagatgtatc aggtttaaca tatggggcag gtaagcctat attgttatca gagtgtgtag
     2041 agtgttgctg cgtacttggt ttcggtggct tatttccccc agccatctcc ccatctacag
     2101 tctgcataag gcgaacagca ggttttttat agccgttatt tttaacccaa tcccatttgg
     2161 tcgggttaag ttctactttt ttacctctcc aaaccgtacc tgc
//
LOCUS       DAWGJV010000208         2193 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWGJV010000208 DAWGJV010000000
VERSION     DAWGJV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.208"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            30..515
                     /locus_tag="WHQ94_001983"
     CDS             30..515
                     /locus_tag="WHQ94_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129207.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDALAFVFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVYGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            611..1234
                     /gene="rsmG"
                     /locus_tag="WHQ94_001984"
     CDS             611..1234
                     /gene="rsmG"
                     /locus_tag="WHQ94_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ2129208.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLEAERHIVILSKR"
     gene            1333..2106
                     /locus_tag="WHQ94_001985"
     CDS             1333..2106
                     /locus_tag="WHQ94_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ2129209.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          422 a          503 c          761 g          507 t
ORIGIN      
        1 tcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 tggcatttgt gttcggacgg acggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgaggagg ctgggacggc gtggtgtggt ttgtgtatgg cgtgtttgcg tttttgaacc
      241 tggtcgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattccc
      301 gttacgcgct gtttctgtcg ggcgtggcgg cttgcgcggc tgctgcggtc gcgtggattt
      361 tcgagctgct gcttggcagt ggggctttgg gcgggctgcg tggggaggcg gtgttggaat
      421 atgcgtttgc cgtgtggctg gtggcgatgc tgacgctgcc caaacgcctg acgcgcgcgc
      481 cggtgcagcc ggtggtgttt cacaggaaaa aataggttgg aacgggaaat gccgtctgaa
      541 ccccgacacg cggtttcaga cggcatgttt ttccgttaac attacgcctg aacatggaca
      601 ggatagggat atggaacgca aagaacgcct gcgtgcaggc atcgccgcat tgggtttgga
      661 tatttccgca gcggcgcagg acaggctttt ggcctatgtg gatttgttga aaaagtggaa
      721 caaaacctac aatctgaccg ccctgcgcga cgaggaaaaa atgattgtcc atcatctttt
      781 ggacagcctg acgctgctgc cccatatcga gggtgtgcaa acgatgctgg atgtcggttc
      841 gggcggcggt cagcccggca ttccggcggc ggtgtgccgt ccggatgtgc agataaccct
      901 tttggatgcg aatacgaaga aaacggcttt tttacagcag gcggttatcg agttgggatt
      961 ggacaatgtg cgcgtggtat ccggacgcgt ggaggcggtt tcggacgtgc gtgccgatgt
     1021 ggttaccagc cgtgcgtttg cagaactggc ggattttgtg tcgtggacgg tgcatctgtt
     1081 gaaagacggc ggctactggg cggcgatgaa gggcgtgtat ccgcaggaag aaatcggccg
     1141 cctgccgcag gatgtgtgcg ttgaaaaagt ccaaaggctc gacgtgccgg gcttggaggc
     1201 ggaacgccat atcgttatcc tgagcaagcg ttgagcgcgc ttcagacggc atgaatacct
     1261 tttttgtgcg gataaaggta aaattccgca ctgtttttct tttttcaaca tcagacggga
     1321 cacgggcggg acatgagtgc gaacatcctt gccatcgcca atcagaaggg cggtgtgggc
     1381 aaaacgacga cgacggtaaa tttggcggct tcgctggcat cgcgcggcaa acgcgtgctg
     1441 gtggtcgatt tggatccgca gggcaatgcg acgacaggca gcggcatcga caaggcgggt
     1501 ttgcagtccg gcgtttatca ggtcttattg ggcgatgcgg acgtgaaatc ggcggcggta
     1561 cgcagcaaag agggcggata cgctgtgttg ggtgcgaacc gcgcgctggc cggcgcggaa
     1621 atcgagctgg tgcaggaaat cgcccgggaa gtgcgtttga aaaacgcgct caaggcagtg
     1681 gcggaagatt acgactttat cctgatcgac tgtccgcctt cgctgacgct gttgacgctt
     1741 aacggcttgg tggcggcggg cggcgtgatt gtgccgatgt tgtgcgaata ttacgcgctg
     1801 gaagggattt ccgatttgat tgcgaccgtg cgcaaaatcc gtcaggcggt caatcccgat
     1861 ttggacatca cgggcatcgt gcgcacgatg tacgacagcc gcagcaggct ggttgccgaa
     1921 gtcagcgaac agttgcgcag ccatttcggg gatttgcttt ttgaaaccgc catcccgcgc
     1981 aatatccgcc ttgcagaagc gccgagccac ggtatgccgg ttatggctta tgatgcgcag
     2041 gcaaagggtg ccaaggcgta tcttgccttg gcggacgagc tggcggcgag ggtgtcgggg
     2101 aaataggtca atccaaatcg ggctgcccgt gcctttatgc tgtttggccg ggtgcgttaa
     2161 tatggcggat taaaataaaa atacttatat cgt
//
LOCUS       DAWGJV010000209         2082 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWGJV010000209 DAWGJV010000000
VERSION     DAWGJV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.209"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            177..1997
                     /locus_tag="WHQ94_001986"
     CDS             177..1997
                     /locus_tag="WHQ94_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ2129210.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLF
                     ATVQANATDDDDLYLEPVQRTAVVLSFRSDKEGTGEKEGTEDSNWAVYFDEKRVLKAG
                     AITLKAGDNLKIKQNTNENTNENTNENTNENTNDSSFTYSLKKDLTDLTSVETEKLSF
                     GANGNKVNITSDTKGLNFAKETAGTNGDPTVHLNGIGSTLTDTLLNTGATTNVTNDNV
                     TDDEKKRAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVE
                     SKDNGKKTEVKIGAKTSVIKEKDGKLVTGKGKDENGSSTDEGEGLVTAKEVIDAVNKA
                     GWRMKTTTANGQTGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGD
                     ALNVNQLQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKN
                     IDIATSMAPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDV
                     TNVAQLKGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGE
                     AGYAIGYSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          648 a          499 c          519 g          416 t
ORIGIN      
        1 ataaaacagt ttccttagat tccacgtccc agattcccgc cttcgcggga atgacgagat
       61 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      121 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      181 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtcgtc gtatccgagc
      241 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      301 tgttgtttgc aacggttcag gcgaatgcta ccgatgacga cgatttatat ttagaacccg
      361 tacaacgcac tgctgtcgtg ttgagcttcc gttccgataa agaaggcacg ggagaaaaag
      421 aaggtacaga agattcaaat tgggcagtat atttcgacga gaaaagagta ctaaaagccg
      481 gagcaatcac cctcaaagcc ggcgacaacc tgaaaatcaa acaaaacacc aatgaaaaca
      541 ccaatgaaaa caccaatgaa aacaccaatg aaaacaccaa tgacagtagc ttcacctact
      601 ccctgaaaaa agacctcaca gatctgacca gtgttgaaac tgaaaaatta tcgtttggcg
      661 caaacggtaa taaagtcaac atcacaagcg acaccaaagg cttgaatttt gcgaaagaaa
      721 cggctgggac gaacggcgac cccacggttc atctgaacgg tatcggttcg actttgaccg
      781 atacgctgct gaataccgga gcgaccacaa acgtaaccaa cgacaacgtt accgatgacg
      841 agaaaaaacg tgcggcaagc gttaaagacg tattaaacgc aggctggaac attaaaggcg
      901 ttaaacccgg tacaacagct tccgataacg ttgatttcgt ccgcacttac gacacagtcg
      961 agttcttgag cgcagatacg aaaacaacga ctgttaatgt ggaaagcaaa gacaacggca
     1021 agaaaaccga agttaaaatc ggtgcgaaga cttctgttat taaagaaaaa gacggtaagt
     1081 tggttactgg taaaggcaaa gacgagaatg gttcttctac agacgaaggc gaaggcttag
     1141 tgactgcaaa agaagtgatt gatgcagtaa acaaggctgg ttggagaatg aaaacaacaa
     1201 ccgctaatgg tcaaacaggt caagctgaca agtttgaaac cgttacatca ggcacaaatg
     1261 taacctttgc tagtggtaaa ggtacaactg cgactgtaag taaagatgat caaggcaaca
     1321 tcactgttaa gtatgatgta aatgtcggcg atgccctaaa cgtcaatcag ctgcaaaaca
     1381 gcggttggaa tttggattcc aaagcggttg caggttcttc gggcaaagtc atcagcggca
     1441 atgtttcgcc gagcaaggga aagatggatg aaaccgtcaa cattaatgcc ggcaacaaca
     1501 tcgagattac ccgcaacggt aaaaatatcg acatcgccac ttcgatggcg ccgcagtttt
     1561 ccagcgtttc gctcggcgcg ggggcggatg cgcccacttt aagcgtggat gacgagggcg
     1621 cgttgaatgt cggcagcaag gatgccaaca aacccgtccg cattaccaat gtcgccccgg
     1681 gcgttaaaga gggggatgtt acaaacgtcg cacaacttaa aggtgtggcg caaaacttga
     1741 acaaccgcat cgacaatgtg gacggcaacg cgcgcgcggg tatcgcccaa gcgattgcaa
     1801 ccgcaggttt ggctcaggcc tatttgcccg gcaagagtat gatggcgatc ggcggcggta
     1861 cttatcgcgg cgaagccggt tacgccatcg gctactcgag catttctgac actgggaatt
     1921 gggttatcaa gggcacggct tccggcaatt cgcgcggtca tttcggtact tccgcatctg
     1981 tcggttatca gtggtaaggg ctttttcgcc tgtctgctgt tgggacaggc ggaaggtttg
     2041 aagggaaggg tggcgatttg ccgcctgaga cctttgcaaa at
//
LOCUS       DAWGJV010000210         2024 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWGJV010000210 DAWGJV010000000
VERSION     DAWGJV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.210"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(28..351)
                     /locus_tag="WHQ94_001987"
     CDS             complement(28..351)
                     /locus_tag="WHQ94_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2129211.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(339..578)
                     /locus_tag="WHQ94_001988"
     CDS             complement(339..578)
                     /locus_tag="WHQ94_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ2129212.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(701..1498)
                     /gene="truA"
                     /locus_tag="WHQ94_001989"
     CDS             complement(701..1498)
                     /gene="truA"
                     /locus_tag="WHQ94_001989"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ2129213.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGESVATTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKHRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          463 a          487 c          569 g          505 t
ORIGIN      
        1 tgaacacaat ccattcagac tattcaatca ggcaaacatc tcctgcaata ctgcaaacag
       61 tttttcagcc gtactgttgt ctaaattgcc aagatgcttg accaatccgg ctttatccac
      121 agccctaatc tgttcgggca aaagcaaacc gtctttatcc tgaaagcgga cattgacgcg
      181 gaacggggca ggacggcttc cgctcgtcat gggaacgatc agcacagtct tgagatagtt
      241 gtgtatttca ggaggagata ctacgacaca aggacgtgtc tttttgattt cgcttcctac
      301 ggtcgggtct aaggagacca gatagattcc gccgcgtact accatatcca ttctttatcc
      361 gcttcgtttt caatttcgga aaaaaaatgc tcctgctcgg tttcgacaag cattgcggca
      421 gcttctgccc atcccctgcg aacggtagga cagcttaaaa tgatattgcc cttttcaact
      481 gtaacagcca agctgtctac tgcccctatt tgacccaata atgatttggg cagaatcacg
      541 ccttgcgagt ttcccatttt gcgtatgttg agaatcatat acgtacccta tttaccagtt
      601 tatgtaataa caatgttatt accttgatga ggtagtgtca atattgaaaa agattgcctg
      661 acagtttgtc cgatttcaaa atctccgcga caagaatgtt ttaaagccat tcggggattt
      721 gggggcggat gatgccgtat gcctcgggat agtcgacgcc ggtcaggtaa agtccgtcgg
      781 gcatgaaggt cggcggggct ttgaggcggc tgcgttcttg aatcagtgcg gcgaagcctt
      841 cgacgctgag tctgccgctg ccgacataga cgagcgcacc catgatgttg cgtaccatat
      901 ggtgcaaaaa ggcgttgccg tgcaaatcga ggcggacgag tcctgcgctt tgggtaaggt
      961 cggcgcggta gatggttttg acgggggatt ttgcctggca ttcggcggcg cggaagctgg
     1021 agaagtcttg ttcgccgatc aataaggcgg cagcctgccg catctgcccg atgtcgagtt
     1081 tgagatgcgt ccagcctgcc ctgtgcttca acagggggga acggacgggg gcggattcga
     1141 gcaggtagcg gtaatgccgt ccgtatgcgt cgaagcgggc gtgaaattcg ggggcgacct
     1201 gtcgggcgtg caaaacggca atgccttcgg gcaggtgggc atttacgccg cgcacccatg
     1261 cctgttgggg acgggcggca gttgtgtcga agtggacgac ttgggcggtg gcatgcacgc
     1321 cggtgtcggt cctgccagca acggtggtgg cgacagattc tcctgcgatt tgggcgagcg
     1381 cggtttccaa tgccgcctga acggtcggta cgccgtcagc ctgtttctgc cagccgtaaa
     1441 agcggctgcc gtcataggat agggttattg cccaacgttg tttttgtgcg gtatccatcg
     1501 gatttgggat tcggataaat gttcagactg cattgtatcg cagattttgc agggaaacgg
     1561 caaacgcccg gggcgagcgg cgttgtttgg ggagttgggg ggggggatgc agttgctacg
     1621 aatcgctatc ctgtgaattc accctgtcag gagtgcccga atcgtcattc ccgcgcagtc
     1681 gggaatctag gacgtaaaat ctaaagaaac cattttatag tggattaaca aaaaccagta
     1741 cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcatcaagtg
     1801 aatcggttcc gtactatctg tactgtctgt ggcttcgtcg ccttgtcctg atttttgtta
     1861 atccactata tccgataagt ttccgcaccg acaaaactag attcccactt tcgtgggaat
     1921 gacgggatgc aggttcgtgg gaatgacgcg aacagaaacc tcaaatcccg tcattcccgc
     1981 gcaggcggga atctagacct tagaacaaca gcaatattca aaga
//
LOCUS       DAWGJV010000211         2006 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWGJV010000211 DAWGJV010000000
VERSION     DAWGJV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.211"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            301..1758
                     /locus_tag="WHQ94_001990"
     CDS             301..1758
                     /locus_tag="WHQ94_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2129214.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGATTGFLLLLLVPKLNKMLEGTD"
BASE COUNT          451 a          601 c          463 g          491 t
ORIGIN      
        1 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta taaaaaaccg
       61 ccctatcgga taagccctta acagaaaagg ctttacccgc gccgtatcgg aacacatcct
      121 ctaaaataca atccgttgaa ttgaaaaaaa tataaaaaca tccgcccgcg aaaaacggca
      181 gcgcgtcgtt tgacaaagaa tgaaaatatc ggttaaaaac cgattttcat acaaaaaaca
      241 ccgctgccgt ccgcatccgt ttcagacggt attgagagaa aatcttttag gagaaccttt
      301 atgtcccggc atcccgcccc caccggagaa aaaacattct tcggccaccc cttccagctt
      361 tccaccctct tccatatcga attgtgggaa cgtttttcat tttacggaat gcagggcatc
      421 ctgctgattt acctctacta caccgccgac aaaggcggct tgggcataga caaaaccctc
      481 gccggcggca ttgtcggcgc atacagcggc agcgtgtacc tgtccaccat tttgggggcg
      541 tggtttgccg accgagtatg gggtgcggaa aaaaccctct tcctctcggg catcgtcgtg
      601 atgctcggac acatcgtcct tgccgccgcc ccgggcctgt acggtctttt aatcgggctg
      661 atattcatcg cattgggcag cggcggtgtg aaatctacgg ccagttctat ggtgggcgca
      721 ttatacgaac aggacgaaat gcgcccgctg cgcgatgcgg gattttccat tttctacatc
      781 gccatcaaca tcggcggctt cctgggcccg ctgctgaccg gcctgctgca ggaaaacatc
      841 ggtttccatt atggtttcgg cgcggcggcg gtcggtatgg cattcggctt gtggcgttat
      901 tccttgggac gtaaaaacct gccccacccc accgtccccc atccgctttc aaaaggacag
      961 ggcaaaactg cggccgccgt cggcatcacc ctcatcgccg cacttgcaac cgccatcaaa
     1021 accgggcttg tcaacctcga caatttctcc ggcatcctct tatctaccgt catccttgcc
     1081 gtcattgcct atttcgcccg cctgctgacc aacccccgcg tcagttccga caacaaacgg
     1141 cacatcatcg cctacatccc gcttttcctg accatctgta tgttttgggc cgtctggttt
     1201 cagatttaca ccgtggcaac cgtctatttc gacgaaaccg tcaaccgcac catcggttcg
     1261 tttaccgtgc ccgtcgcctg gaaagattcc atgcaaagcc tgtgggtcat cctgttttcc
     1321 ggactgatgg cggcaatgtg gacaaaaatg gggcgcaaac agcccaaaac cccgctgaaa
     1381 ttcgctatgg cggtatttgt taccggcgcg tcgtttttgg gattcgtccc ctttatttcc
     1441 gccggcacgc cgatgcctat tgcggttttc gcactgattg tccttgccat cacgataggc
     1501 gaactgatga tttccccgat tgcgctgtcc atctccacca aaatcgcacc gcctttattc
     1561 aaaacccaaa tggtcgccct taatttcctt gccttttcat taggcttcac tttgggcggc
     1621 gtattgtttg agaaaggcta tcaggcgggc gacgaaatcg gcttctaccg gctgctgttc
     1681 tacatcggcg caaccacagg cttcctgctg ctcctgctcg tccccaaatt gaacaaaatg
     1741 ctcgaaggca cagactaagt cccgccccga tgcgccgatg ccgtctgaac ccttcagacg
     1801 gcatttttcc gcataatgaa accaaaccgt ttccacccga caggacaggc tccccccaac
     1861 cggaaggcag cctgccgatt gtcatttgaa tatctgactg attccgtctg tttgcaaggg
     1921 aaatagccgt ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat
     1981 ccgactaaat tagtgctgca tcaacg
//
LOCUS       DAWGJV010000212         1884 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWGJV010000212 DAWGJV010000000
VERSION     DAWGJV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.212"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(127..1437)
                     /locus_tag="WHQ94_001991"
     CDS             complement(127..1437)
                     /locus_tag="WHQ94_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ2129215.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKSLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPYVENGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLGAAETR
                     RLKTLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDCLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          454 a          493 c          480 g          457 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagca
      241 gtcgggcata tagctttcgc cggcggcgta ggcattcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcattgcgca ggtggacggg
      361 tacttcgtcg ctggcgtttt ctttgacgaa acggcgcatt tgattgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt gcaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcagtca gggttttcag
      781 acggcgcgtt tcagcggcgc ctaacagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaaggattg
      961 caaaacatac acctgcgcgc ggctcaacag cgcgggattc acttcaaacg acggattttc
     1021 cgtcgttgcg ccaataaagg tcagcaaacc gttttccaca taaggcaaaa acgcatcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgccc tcccctgctg
     1141 caaagcgatt tcagccttgt cgattgcccc gcgtatgtct tttacgccgg aaaacacggc
     1201 ggaaacaggc agaaactggg cattgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccccctt ctaccgccac
     1321 gcgcaaagat ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcaccggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 tgaaaacttt agaaaattag aact
//
LOCUS       DAWGJV010000213         1869 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWGJV010000213 DAWGJV010000000
VERSION     DAWGJV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.213"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..137)
                     /locus_tag="WHQ94_001992"
     CDS             complement(<1..137)
                     /locus_tag="WHQ94_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ2129216.1"
                     /translation="MSTFFQQTSQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            178..606
                     /locus_tag="WHQ94_001993"
     CDS             178..606
                     /locus_tag="WHQ94_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2129217.1"
                     /translation="MGNFAKVSGYTYNKSRYRNAAEVNAERLAKNSSADPYNFSNFTP
                     VHIFRFGTSFHIPNTGLTVGGGVSAQSSTSSLYNIRQGGYGLIDGFVRYELGKHAKLS
                     LIGTNLNGHTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            749..1807
                     /gene="dinB"
                     /locus_tag="WHQ94_001994"
     CDS             749..1807
                     /gene="dinB"
                     /locus_tag="WHQ94_001994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ2129218.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          444 a          495 c          514 g          416 t
ORIGIN      
        1 aggtaacggg ttttttgacg attcaggtac tgttcgatcg gctgccaatc aattacctgg
       61 tccaacttca atagcgggaa acggtcgatg tgtttggcaa tcatggcttg ggaggtttgc
      121 tggaagaagg tgctcatgga aaatccccta aatggcttgg tgggaattta ggggattttg
      181 gggaattttg caaaggtctc aggttatacc tacaacaaga gcaggtatag gaatgcagcc
      241 gaagtcaacg ccgaacgcct tgccaaaaat tccagtgcag acccgtacaa cttcagcaat
      301 ttcacacccg tgcatatatt ccgtttcgga acgagtttcc atataccgaa tacggggctg
      361 accgtcggcg gcggcgtgtc cgcacaaagc agcacaagca gcctgtacaa catcaggcag
      421 ggcggctacg ggctgataga cggtttcgtc cgttacgaat tgggcaaaca cgccaaattg
      481 agcctcatcg gtacgaactt aaacggacac acttattttg agaacaacta caaccgtacg
      541 cgcggcgcaa acaacttcta tggagagccg cgcactgtca gcatgaaact ggattggcag
      601 ttttaattga aaaccgtagt tttgcggatg ccgtctgaac agggtatgtt tcagacggca
      661 tttttatgga ggcaggccta tcggggcgta tatttggaat ttgctcgatg ccggcagtag
      721 aatatccttt ttatcttcag cagagccgat gtcttcacgc aaaatcatcc acatcgacat
      781 ggacgcgttc tacgcatcgg tagagctgcg cgaacagccg catttgaaag ggcggccggt
      841 ggtcgtcgcg tgggagggcg cgcgttcggt gatttgcgcc gcatcgtatg aggcacggca
      901 gttcgggctg cattccgcga tgtcggtggc aacggcgaaa aggctgtgtc cgcaagcggt
      961 gtatgtgccg ccgcatttcg atttgtaccg tcaggtttcc gcgcagattc acgccgtatt
     1021 caggcgttat accgatttaa tcgaaccctt gtcgctggac gaagcctatc ttgacgttac
     1081 ccgtaatttc aaaaacatcc cttacgccag tgaagttgcc aaagaaatcc gtgccgccat
     1141 ttttgcggaa acaggtttga ctgcatccgc aggcatcgcg ccgaacaaat ttctggcgaa
     1201 aatcgcgtcg gactggcgca agccgaacgg gcagtttgtg ttgccgccgc acaaagtcat
     1261 ggcatttttg gaaaccctgc ctttgggcaa aatccccggc gtgggcaagg taacgctgaa
     1321 aaaaatgcag tcgctgggta tgcggacggc gggcgacttg cgccgtttcg agcgcggcga
     1381 actcttaaac catttcggac gctacggata ccgcctctat gatttggtgc gcggtacgga
     1441 cgaacgcccc gtcaaagccg aacgcgaacg cctccaaatc tccacagaaa ttaccctgcc
     1501 cgaagacctg ccgctcgagc aggctgccgg acacctcccc catcttgccg aagacttgtg
     1561 gcggcaaatc acgcgcaaaa acgtcgaagc ccaaagcgta acgctcaagc tgaagaccta
     1621 cgatttccgc atcatcacgc gcacactgac ttattcctcc gtattgcccg actgcgcagc
     1681 tctgctgcag gctgcgcaaa tgttgatggc gcgcgtcccg ccgcagacgg aagacgcgtt
     1741 ccgccttatc ggtatcggcg tggggcatct tgtgccgaaa aaccagcagc aggatttgtg
     1801 ggcgtaaacc gctttacgcg cgccgtccaa aaaatatgcc gtctgaacgg ttttcagacg
     1861 gcatggctg
//
LOCUS       DAWGJV010000214         1855 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWGJV010000214 DAWGJV010000000
VERSION     DAWGJV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.214"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            212..1684
                     /gene="pyk"
                     /locus_tag="WHQ94_001995"
     CDS             212..1684
                     /gene="pyk"
                     /locus_tag="WHQ94_001995"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ2129219.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          481 a          537 c          501 g          336 t
ORIGIN      
        1 ccaaaaataa atttagccta agtcataaaa ccgtgtaatt ttgcaccaaa aaagacggtt
       61 tgcaggtaaa atatcaaact attaatttaa atcatatttt tacagaatat tccgccgcat
      121 tcatcaaatg gacatcaaac cggttccaaa tttctgtaat tttgcaacaa aataccgcaa
      181 aacacccgat tgagaccgaa aggactttca tatgaaccag acaagccgcg atctgacccg
      241 catcagccac aacaccaaaa tcgtcgccac cttggggccg ggcagcaaca acgtcgaact
      301 gttggaagac atgatccgcg tcggcggtct gaacgtcgtc cgcttcaact tcagccacgg
      361 cacgcccgaa ttccatcagg aaaacgcccg cattgtgcgc gaggcggcaa aacgcgccgg
      421 acaggaaatc gccatcattg ccgacctgca agggccgaaa atccgcgtgg gcaaaatcgc
      481 cggcggcggc atcgaattga acaaaggcga aacgctggta ctggatgccg cgctcgaagg
      541 cgaaggcacg cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg acgttaccgc
      601 aggcgacgta ttgtggctgg acgacggcct gctgaccctg accgtggaat ccgtcgaagg
      661 cagcaggatt atcacaaggg tggaaaacag ccatgtcctg aaaagcaaca aaggcatcaa
      721 caaacgcggc ggcgggctgt ccgcaggcgc gttgaccgaa aaagacttcc gcgacctgaa
      781 aaccgcgatt gccatcggtt gcgactacct cgccatcagc tttgtgaaat ctgccgaaga
      841 tttgcacatc gcgcgcgcca aagtcgaaga agaaatgaag ggcagcactg ccgtgcgccc
      901 cggtttggtt tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg aaatcatcct
      961 cgccggcgac ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg gacacgccgc
     1021 cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc gcttcagcat
     1081 tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc gcgcggaagt
     1141 cagcgatgtg gcaaacgcgg tattggacgg taccgatgcg gtgatgtgtt ccgccgaaac
     1201 cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcaattatct gcgcggctgc
     1261 ggaaaaagag caggattcgc tcaacggcgt tgccgaacag gcggagtatc ccgaagcggt
     1321 cagcaccaac ctggcggttg ccggcggcgc ggtcagcgtg gcgcgcgcgg ttcacgccaa
     1381 agcgattgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc gccacaacat
     1441 caccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta tggcgatgta
     1501 ccgcggcgtg cgcccgctga ttttggcaac cagtaccgac cacgacacgg cgttgaacga
     1561 agtcgaaacc atgctggtgg aacacaacat cctgcattcc ggcgaccaat acatcatcac
     1621 cagcggctcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc tgcgcgtcaa
     1681 ataatccgcc ctgagtggaa aatgccgtct gaagtcgatg cccgaggctt cagacggcat
     1741 tttttgcggc ggcgcggcgg ttcaggcaaa ctgaaaaaat tgtgcaaatc cggcaacatc
     1801 agataaaatt gagtacctat actaaagcga aacaaggcat ttccgactgc ctttt
//
LOCUS       DAWGJV010000215         1849 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWGJV010000215 DAWGJV010000000
VERSION     DAWGJV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.215"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            72..1241
                     /gene="metZ"
                     /locus_tag="WHQ94_001996"
     CDS             72..1241
                     /gene="metZ"
                     /locus_tag="WHQ94_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ2129220.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVANLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1327..1533
                     /locus_tag="WHQ94_001997"
     CDS             1327..1533
                     /locus_tag="WHQ94_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ2129221.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          447 a          491 c          475 g          436 t
ORIGIN      
        1 tgttaatcca ctataaatta aacaacgggc gcacgctccg ttgccgaacg gataaaaccg
       61 aggaattacc gatgagcaaa aaactccatc cgcaaacgct cgccatacgc ggcggcaaag
      121 aacaaaccga ataccgcgaa cacaatcagg ccttgttcct gaccagcagc ttcatgtggg
      181 acaacgccca acacgccgcc gatttgtttt caaaaaaaat caaaggcttc acttataccc
      241 gtaccgccaa cccgaccaca gccgcctttg aaaaacgcat cgccgctttg gaaggtgcgg
      301 aacgcgcggt tgccacttcg accggcatgt ccgcgattca ggcggcgttt ttcacctttt
      361 tgcaggcggg cgaccatgtg gtttccagcc gcagcctgtt cggcacgacc gtcggcttta
      421 tcaataacat cgttaccaaa ttcggcatcg aagtgagcca cgtgtcgccg actgatataa
      481 acgaatggaa agccgccgtc aaagccaata ccaaactgct gtttttggaa acgccgtcca
      541 accccttggg cgaagtggcc aacttggagg cattggcgga attggcgcac ggcatcggcg
      601 cgattttggt ggtggacaac agcctgctgt cgcccatcgg ctctcagcct ttgaagcacg
      661 gcgcagatat ttccgtttct tccgcaacca aagccataga cggacacgga cgcgtgatgg
      721 gcggcgtgtt ggcgggttcg gaagaactga tggcgcaggc cgccgtttac tgcaactctt
      781 gcgggctggc gatgtcgccg tttaacgcgt ggcagttgtt gagcggcgtg gaaaccctgt
      841 cgctgcgtat ggaaaaacag ttcgacaacg ccctgaaaat cgcacaatgg ctgcaagcgc
      901 agccgcaggt tcaagccgtg tactacaccg gattgcccga ccatccgcag gcggcgctta
      961 tccgcaaaca gcaaaacggc ggcggcatcg tcatcggctt tgaagtcgcc gaccaagcag
     1021 ccgcgtggaa agtcgtggac ggcgtggaac tcttttcccg taccgccaac ctcggcgacg
     1081 tgcgctcgac catcacccat ccgtggacaa ccacgcacgg caggatgcag cccgaagaaa
     1141 aactcgccgc caacatccgc cccggattgg tgcgcctgtc ggtcggtttg gaatatgttg
     1201 gcgatttgat tgacgatttg aaacaggcac tcgcacgctg attgcgtgtt tcagacggca
     1261 ttggacagca gatgccgtct gaaaccctta tcgcgtatgg catacacatc aacaatagga
     1321 aaaaatatgg atgaagcggt ttttgccgat tgggcgttga aaatctgcct gaccggtttg
     1381 attatttttt tgggttttat cgtttggaat ttgggcaagg agtccaaagc gggcaaattc
     1441 ggcatcgcca ttttgttttt ggttttgggc ttgggcgtgt tcggttttgt atttaaagaa
     1501 ctgctaatca agtttttggt attgcccaaa taggctgccg cctgtcaaaa tgctgtctga
     1561 aatcataaaa agtaatctgc accctaaaag tttgatttaa ccgccaactg attaaggtgc
     1621 ggatttttat gacaaaataa aaatcatctt tccgcagtca ttcccacgga cgcgaaagtc
     1681 tgtctttttg aatcccggat aattttacat cgtcattccc gcgcaggcgg gaatctagtc
     1741 tgttcggttt cggtcattta cgataaattc ctattgcttt tcatttcctg attcccactt
     1801 ccgtgggaat gacggcggag cggataaatt ctcgcaatct aaaacctcg
//
LOCUS       DAWGJV010000216         1808 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWGJV010000216 DAWGJV010000000
VERSION     DAWGJV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.216"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(48..1559)
                     /gene="ubiB"
                     /locus_tag="WHQ94_001998"
     CDS             complement(48..1559)
                     /gene="ubiB"
                     /locus_tag="WHQ94_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ2129222.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVCLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(1594..1806)
                     /locus_tag="WHQ94_001999"
     CDS             complement(1594..1806)
                     /locus_tag="WHQ94_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ2129223.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
BASE COUNT          420 a          499 c          490 g          399 t
ORIGIN      
        1 cggtttcctt ataaacgggc ggagtcgggc aatgattcgg acaaaaccta tttcaaaagc
       61 aaaatcagca gcaaaacaac cgctatcgcc gccagccaca ggctttgccg ctgctgcact
      121 ttgaccaaat ggatataggc atcacgcatt tcctgctggc ggttttcatc aatcaacgca
      181 ctgattttgc gcggcaatga aggaatgatt tgcgcccagt cgggggcttc gtttttgagg
      241 ttgcgccaaa gggctttagg gccgacctgc ccgttcatcc atttcaccaa aaacggtttg
      301 gcggttttcc acaagtccaa atcgggatca agctgccgtc ccaagccttc gatgttgagc
      361 agcgtttttt gcagcaatac cagctgcggc tggatttcga cattgaagcg gcggctgact
      421 tcaaacaggc gcatcagcac caagccgaag gaaatctgcg aaatcggttt gttgaacact
      481 ggttcgcaca cggcgcggac agccgcttcc aactcttccg cgcgcgtgtc ggcgggcacc
      541 cagcccgatt cgatgtgggc ggtggcgacg cgccggtaat cgcggttgaa gaaggcgagg
      601 aagttgatgg cgagataacg tttgtcgtaa tcggtcagcg tgccgacgat gccgaaatcg
      661 agggcgatgt agcggttgtc ggcggcaacc aaaatattgc cggggtgcat atccgcgtgg
      721 aaaaaaccgt cgcggaagac ctgcgtgaaa aagatttcca cgccgtaatc ggcgagtttg
      781 tgcaaatcga tgccgtctgc tttgagtttg gcgatgtcgg aaaccggcgt gccgtccatc
      841 cattcgatgg tcagcacgtc gctggtgcag tagtcgtaaa acaccttcgg cacaatcagc
      901 atatcgctgt tttggaaatt gcgtccgagc tggctggcat tggcggcttc gcgcatcaag
      961 tccaactcgt cgtgcagata tttgtcgaac tccgccacca cttcgcgcgg cttcagacgc
     1021 ttgccgtcgg caaacagacg ctcgacccag cctgcaccaa agcgcatcag cgacaaatcc
     1081 tgttcgatca cgggcaaaag gttggggcgc aagactttga ccgccacttg ttcgcccgaa
     1141 tgcaggcggg ctttgtgtac ttgggcgatg gacgcgctgg cgatgggctc ggtttcaaat
     1201 tccgcataca gcttttcgat gggctgaccc agcgattttt cgatttgttc acgcgaaagc
     1261 cgcgcgtcaa aaggcggcac tttgtcttgc agctttgcca gttcgacggc gtaatcgtgc
     1321 ggaatcaaat cggggcgtgt ggacaaaacc tgcccgaact tgatgaaaat cggccccagg
     1381 ctttccaagg caaggcgcag acagacggca ggcggttcgt ttttcaattt ggacgactgc
     1441 ggcatcattt tcagcagagc gcatatccaa ccgctaccca tcagcgaagc acacagacct
     1501 gccagccgat agcggtaaaa agtcccgaca atgaccgtca ggcgtttcaa ccatttcata
     1561 tataaaatcc cgaatacagc atttatcgga caatcaaaaa atcttaccga gcagatagcc
     1621 caaaaaggca atcagcagcc cgaaaagcag cggtttgata ttgcgggtcg gcggttcggg
     1681 ttgcgaaggt gcggaaatat catcttcccg cccgccatga agttcaagat attcgggcat
     1741 ttccccatcc gcgccctctc cttcgccctc accttcctcc ggcggcacga ccgtttctaa
     1801 ctccatat
//
LOCUS       DAWGJV010000217         1790 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWGJV010000217 DAWGJV010000000
VERSION     DAWGJV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.217"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(148..804)
                     /locus_tag="WHQ94_002000"
     CDS             complement(148..804)
                     /locus_tag="WHQ94_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ2129224.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="WHQ94_002001"
     CDS             869..1759
                     /locus_tag="WHQ94_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ2129225.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT          402 a          421 c          588 g          379 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc cgtatgctcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cctgtgccga cttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggttggac
      541 gtgttcggga ataccgtttt tcatatactc tctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct
      721 ataccacata aagggtaggg atgcggcaag cagccagtgg aaaaggcggg tggggaagtc
      781 ccagactttg gttttgtttt tcataattgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcatcgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggcgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggttttcccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcttggatgg taggttgcgg
     1201 cctgggtacg gataaagcgg cggtcggaac gcttgccgga attttggcgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggcg cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aggggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgctgcgtc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgcgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggca gggctgttgg caggggaaat cgctccggcg gcaaggtggc tgcgcaaccg
     1741 gataactaaa agtatgtaag aagatatagt ggattaacaa aaaccagtac
//
LOCUS       DAWGJV010000218         1776 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWGJV010000218 DAWGJV010000000
VERSION     DAWGJV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.218"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            298..1464
                     /locus_tag="WHQ94_002002"
     CDS             298..1464
                     /locus_tag="WHQ94_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ2129226.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGNNIQLQLTEPP
                     SKGQTGNKVTKGKSRIRTKINDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATQ
                     WGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVS
                     VRYDSPDFSGFSGSVQFVPAQNSKSAYTPATYTVDSRGVVTPVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFNLFLLGRIGEDDEAKGTDPLKNHQVHRLTGG
                     YEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGK
                     KGEHTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          453 a          409 c          456 g          458 t
ORIGIN      
        1 agtgccgcgt gtgttttttt atggcgtttt aaaaagccgg gactgcatcc aggcagcagt
       61 gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa
      121 aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt
      181 atcagatatt tgttctgaaa aatggttttt tgcggggggg gggggtataa ttgaagacgt
      241 atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga agccgatatg
      301 cgaaaaaaac ttaccgccct cgtattgtcc gcactgccgc ttgcggccgt tgccgatgtc
      361 agcctgtacg gcgaaatcaa agccggcgtg gaaggcaaca acattcagct gcaattgacc
      421 gaaccaccct caaaaggtca gacgggcaat aaagttacta agggcaaaag ccgcatcagg
      481 acgaaaatca acgatttcgg ctcgtttatc ggctttaagg ggagcgagga tttgggcgaa
      541 gggctgaagg ctgtttggca gcttgagcaa gacgtatccg ttgccggcgg cggcgcgacc
      601 cagtggggca acagggaatc ctttatcggc ttggcaggcg aattcggcac gctgcgcgcc
      661 ggtcgcgttg caaatcagtt tgacgatgcc agccaagcca ttgatccttg ggacagcaac
      721 aatgatgtgg cttcgcaatt gggtattttc aaacgccacg acgatatgcc ggtttctgta
      781 cgctacgact ctccggactt ttccggtttc agcggcagcg tccaattcgt tccggcccaa
      841 aacagcaaat ccgcctatac gccggctact tatactgtgg atagtcgtgg tgttgttact
      901 cccgttcctg ctgttgtcgg caagcccgga tcggatgtgt attatgccgg tctgaattac
      961 aaaaatggcg gttttgccgg gaactatgcc tttaaatacg cgaaacacgc caatgtgggc
     1021 cgtgatgctt ttaatttgtt cttgcttggg cgcatcggcg aggatgatga agccaaaggt
     1081 accgatccct tgaaaaacca tcaggtacac cgcctgacgg gcggctatga ggaaggcggc
     1141 ttgaatctcg ccttggcggc tcagttggat ttgtctgaaa atggcgacaa aaccaaaaac
     1201 agtacgaccg aaattgccgc cactgcttcc taccgcttcg ggaatgcagt tccacgcatc
     1261 agctatgccc atggtttcga ctttatcgaa cgcggtaaaa aaggcgaaca taccagctac
     1321 gatcaaatca tcgccggcgt tgattatgat ttttccaaac gcacttccgc catcgtgtct
     1381 ggtgcttggc tgaaacgcaa taccggcatc ggcaactaca ctcaaattaa tgccgcctcc
     1441 gtcggtttgc gccacaaatt ctaaatatcg gggcggtgaa gcggatagct ttgtttttga
     1501 cggcttgcct tcattctttg attgcaatct gactgccaat ctgcttcagc cccaaacaaa
     1561 aatccggata cggaagaaaa acggcaataa agacagcaaa taccgtctga aagattttca
     1621 gacggtattt cgcattttcg gcttggtttg cacatatagt gggggactgt tagtttttgc
     1681 acaaggaaca aatagagtaa aaaaacgctg aaatctcgga aagacgtgga tttcggcgtt
     1741 tttttgcatc cggaaaagtt acgccagctt tttcac
//
LOCUS       DAWGJV010000219         1751 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWGJV010000219 DAWGJV010000000
VERSION     DAWGJV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.219"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            170..1144
                     /locus_tag="WHQ94_002003"
     CDS             170..1144
                     /locus_tag="WHQ94_002003"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ2129227.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALCGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1303..1521)
                     /locus_tag="WHQ94_002004"
     CDS             complement(1303..1521)
                     /locus_tag="WHQ94_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ2129228.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT          479 a          490 c          379 g          403 t
ORIGIN      
        1 ccactataag aataaatatc ccctcatccg cctgccgcgt tccgacttgc cccgcgccgt
       61 ttttttccac tacaatccca cgcgatatct ggcggaacgc accaaaggaa cgccccgcag
      121 caatctgccg cctttcagac ggcacaaaca tttccgaaac gagcaaacta tggacacact
      181 gacccgattc ctccccgaac acctgcaaca aaaccagctg cccgaagcac tctgcggcgt
      241 tttactgtcc gttgtttccg cctgcaccga aatcaacgcc aaagtccgcc tcggcgcact
      301 ggccggcgta ttgggtatgg cgggcacggg caatattcag ggcgaagacc aaaaaaaact
      361 ggatgttatc gccaacaaca tcatgattga cacactcaaa gccaaccctg ccgttgccgg
      421 tttggcaagc gaggaagaag actctttcgt aagtgccggt gaaaacggac gctatctcgt
      481 cctattcgac cctttagacg gatcgtccaa tattgatgtc aacatttccg tcggtacaat
      541 tttctccatc cttgaaaaac ccgaaggcgc attggcaacc gaatcattcc ttcaaacggg
      601 cagacagcag cttgccgctg gttacgttct ttacgggcct caaacccagc tcgtattcac
      661 attcggacac ggcgtatata tgttcacgct caatgccgaa aacgaatttg tgctgacaaa
      721 agaaaaaccg aaagtacccg aaagtaccaa agaatttgcc atcaatatgt ccaaccgccg
      781 ccactggctg ccccccgtcc aacaatacat cgacgagctc ttggcgggcg aaaccggcac
      841 gcgcggcaaa aactacaata tgcgctgggt agccagcatg gttgccgaaa tccaccgcat
      901 actgatgcgc gggggcgtgt tcatgtatcc gcaagacaaa cgcgacccgt ccaaacccgg
      961 caaactgcgc ctgatgtatg aagccaatcc tatggccctg atactcgaac aagccggcgc
     1021 atcggcaagc aacgcccgtc aggacatact cggcatccgt cccgaaagcc tgcaccagcg
     1081 cgtcgccgtc attatgggca gcagcgaaga agtggattac ctcaaccgcc tgcattcaaa
     1141 ataaaaggca gcctgaccga taaagaaagg cggcagcgcg catccgacgc tgccgccgat
     1201 atttcgttta tagccttaaa aagatagaaa accacctttc cgaacggcaa aaatgccgtc
     1261 tgaaaagttt tcagacggca ttttaaaaat taaagcccga atttatttgc ccgctgcttt
     1321 ttgcaggatg gcgtacaact cgtctttcag tttcaatttg gcttttttca gctcatcgat
     1381 ggtttccgca ccgctggtaa ccggattgtt gaccagaccg gtaattttat cgtccagctc
     1441 gttgtgttcg tcgaacagac gggcgaagcg ggaattttcc tgtttcaatt tggaaatcaa
     1501 atcacggtat tctgggaaca tatcgctttc ctctcttggt ttacggttaa aaagtcaaac
     1561 tgaatcttta cattaattat aacaaagata atatttttat ccagtgtttt atcggatacg
     1621 gcaaaatttt cttttaacac cgccgtccgg ttgataaaaa cgttgacaat atcacgcttt
     1681 acgcataaat tttcagcctt tcctactgta tagtggatta acaaaaatca ggacaaggcg
     1741 acgaagccgc a
//
LOCUS       DAWGJV010000220         1732 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWGJV010000220 DAWGJV010000000
VERSION     DAWGJV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.220"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            356..1660
                     /locus_tag="WHQ94_002005"
     CDS             356..1660
                     /locus_tag="WHQ94_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ2129229.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          355 a          433 c          511 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggaatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga ta
//
LOCUS       DAWGJV010000221         1710 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWGJV010000221 DAWGJV010000000
VERSION     DAWGJV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.221"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            61..1434
                     /locus_tag="WHQ94_002006"
     CDS             61..1434
                     /locus_tag="WHQ94_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ2129230.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1614..>1710
                     /locus_tag="WHQ94_002007"
     CDS             1614..>1710
                     /locus_tag="WHQ94_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ2129231.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          441 a          558 c          410 g          301 t
ORIGIN      
        1 tccccatcct atcggtttcc cgcttcagac ggcataaggt ctgaaagaaa gactacaatt
       61 atgagtaatc cattttcctc tttaggtttg ggtacggaac tcgtttccgc actgaccgcg
      121 caaggttacg aaaacccgac gcccatccaa gccgccgcca ttcccaaagc actcgccggt
      181 catgatttac tagccgccgc gcaaaccggc acgggcaaaa ccgccgcctt tatgctgccc
      241 tcgctcgaac gcctcaaacg ttacgccacc gccagcacct cgcccgcgat gcaccccgtg
      301 cgtatgctcg tgttgacccc cacgcgcgaa ctcgccgacc aaatcgacca aaacgtgcag
      361 tcctacatca aaaacctgcc gctgcgccac accgtcttgt tcggcggtat gaatatggac
      421 aaacagaccg ccgatttgcg tgccggctgc gaaatcgtcg tcgccacagt cggacggctg
      481 ctcgaccacg tcaaacaaaa aaacatcagt ttgaacaaag tcgaaatcgt cgttctggac
      541 gaagcagacc gcatgttgga tatggggttt atcgacgaca tccgcaaaat catgcagatg
      601 ctgccccgcc aacgccaaac cctgctcttt tccgccacct tctccgcacc gatacgcaaa
      661 ctggcgcaag acttcatgaa cgcgcccgaa accgtcgagg ttgccgcgca aaacaccacc
      721 aacgccaacg tcgaacaaca catcatcgcg gtcgatacca ttcaaaagcg caacctgctc
      781 gaacggctga ttgtcgattt gcatatgaac caggtcatcg tgttctgcaa aaccaaacaa
      841 agcgtcgacc gcgtaacgcg cgaactggtg cgccgcaacc tgtccgcaca ggcgatacac
      901 ggcgaccgtt cccaacaaag ccggctcgaa acgctcaacg ccttcaaaga cggcagcctg
      961 cgcgtacttg tcgccaccga catcgccgcg cgggggctgg acattgccga actgcccttc
     1021 gtcatcaatt acgaaatgcc cgcccagccc gaagactacg tccaccgcat cgggcgcacg
     1081 gggcgcgccg gtgcggacgg cgtggcgatt tccctgatgg acgaatccga acagaaaatg
     1141 tttgaatcca ttaaagagct gaccggcaac aagctgctca tcgagcgcat cgaaggcttc
     1201 gagccgcaat ggtgggaaca gggcggcgca aaaccggaaa aacccgaaat gcgcgaaccg
     1261 agacaacgca accgctacga atccgccaaa gcacaacgcg aaaaaaacac ccggccggaa
     1321 aatgcggcaa acgatgcggg cgcggcttgc ggaaaaatcg ccggacgcag ccgccgaagc
     1381 cgccgggaac accggacgtg cgccctgctc caaccgcgtt acggcgtaaa ataaccctga
     1441 aaatcaaatg ccgtctgaac atttcccgtt tcagacggca tttttcaaac cggactgacg
     1501 catcgggagc aaccgcccgc accggataaa tttcttccgc aaacagtttc agacggcatt
     1561 tgctgcctgt acaatatcat ttccttttcc actcaaacca tcaggatttc ccaatgtcca
     1621 cccaattcga tgtcgccgta atcggcgcag gtccggcagg ctcggtcgca tccgccctgc
     1681 tccgcaaaaa aggttatagt ggattaaatt
//
LOCUS       DAWGJV010000222         1698 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWGJV010000222 DAWGJV010000000
VERSION     DAWGJV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.222"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            557..1480
                     /locus_tag="WHQ94_002008"
     CDS             557..1480
                     /locus_tag="WHQ94_002008"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ2129232.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          445 a          411 c          400 g          442 t
ORIGIN      
        1 ttattgaaga tggctgaaat tcaaagctac agaaatttat aaacaaaaaa cagcaacccg
       61 ccgccgtcat tcccgcaaaa gcgggaatct agacattcaa tgctaaggca atttattggg
      121 aatgactgaa actcaaaaaa ctggattccc actttcgtga gaatgacgga atgtaggttc
      181 atgggaatga cgggatttaa gtttcaaaat ttattctaaa taactgaaac tcaacgaact
      241 agattcccac tttcgtggga atgacgaatt ttaggtttct gttttgggtt ttctgttttg
      301 gttttatgtc cttgtgggaa tgacggttta cgcgaaaatg ccgtccgaaa ggttttcgga
      361 cggcattttt tgcgtttttc aggagggggg ggcggcttcg tcgccttgtc ttgatttttg
      421 ttaatccact atacattttt atgcggctta cgtattgaaa acggatacat tggcaaatac
      481 tatggaaaaa catagcttgt ttttccggtt ttatcgtatc attccccaac ttaccccatt
      541 gataaggaag cggtttatgc tgcaaagaac tttggcgaaa tccatcagcg ttaccggagt
      601 cgggctgcat tcgggcgaac gcgtcgcact gaccctgcac cccgcgcctg aaaacagcgg
      661 gatttccttc cgacgcaccg atttggacgg cgagatgggc gaacaaatca agctgacccc
      721 ttatttgatc aacgataccc gcctttcatc caccatcgtt accgacaaag gcgtgcgcgt
      781 cggcacgatc gaacacatta tgtccgcgct gtccgcctac ggtatcgaca acgcactgat
      841 tgagctgaac gcgcccgaaa tcccgattat ggacggctcc agcctgccgt ttatttacct
      901 tttgcaagat gcgggcgtgg tcgatcaaaa ggcgcaaaag cgttttttga aaatcctcaa
      961 gcctgtcgaa atcaaagaag cgggcaaatg ggtgcgcttt acgccgtatg acggctttaa
     1021 ggtaacgctg accatcgaat tcgaccatcc ggttttcaac cgcagctcgc ccacttttga
     1081 aattgatttc gccggcaaat cctacatcga cgaaatcgcg cgcgcgcgca ctttcggctt
     1141 tatgcacgaa gtggaaatga tgcgcgccca caatctgggt ttgggcggca atttgaacaa
     1201 cgccatcgtg attgacgaca cggatgtcct gaatcctgaa ggcttgcgct atcccgatga
     1261 gtttgtgcgc cacaaaatcc ttgatgccat cggtgatttg tatatcgtcg gacatccgat
     1321 tgtcggcgcg tttgaaggct acaaatcggg acacgccatc aacaacgcac tcttgcgcgc
     1381 ggttttggca gacgaaacgg cttacgaccg ggtggaattt gccgacagcg atgatttgcc
     1441 cgacgcattt cacgagctga acatcagaac ttgtggataa cgctttctat tttttccggt
     1501 aaattcctaa aacttaaaat ttcatcattc ctacaaaaac agaaaaccaa aatcagaaac
     1561 ctaaaatccc gtcattccca cgaaagtggg aatccaggac gcggaatctc aagaaaccgt
     1621 tttacccgat aagcgtccgc accgacaagt ctggattccc gcctgcgcgg gaatgacggg
     1681 atttgaggtt tctgtttt
//
LOCUS       DAWGJV010000223         1573 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWGJV010000223 DAWGJV010000000
VERSION     DAWGJV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.223"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            40..138
                     /locus_tag="WHQ94_002009"
     CDS             40..138
                     /locus_tag="WHQ94_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ2129233.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(209..1528)
                     /locus_tag="WHQ94_002010"
                     /pseudo
     CDS             complement(209..1528)
                     /locus_tag="WHQ94_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT          289 a          429 c          507 g          348 t
ORIGIN      
        1 cttgccggat gctgcaaaca acgccggttt gcattcctga tggcggtggt tttcttagac
       61 gaacgcccga acacgcagga atggataggc ttggggctgg ttacggcggg cgtgttgacg
      121 ctggcactga aacggtaaag ccgcaagaaa taaatgaaat gccgtctaaa aaactgtttt
      181 cagacggcat tttcgtttct gtccatcctc agcactcgac cacgcgcacg gatacgggga
      241 cggctttttt ccggagcgtg gcggcgagtc cggcaagcga ggtttctttg taggtcgcct
      301 tcatatcccg gccggtttgc agcatggttc ggatgacttc gtcgagcgag acttttttgt
      361 ccgtgccgtc ttccaaaagc gcgagcgtgc cgagtttgag ggctttttcg gcggcgatgc
      421 cgttgcgctc gatgcagggg atttgcacca gtccgccgac ggggtcgcaa gtcagcccca
      481 aatggtgttc catcgccatt tcggcggcgt tttccacttg tttgggcgtg ccgccgatga
      541 cttcggcgta tgcgcccgcc gccatcgaac acgctacgcc gacttcgccc tgacagccga
      601 catccgcacc ggaaatggag gcgttggtct tgtagaggat gccgattgcg cctgcggtga
      661 gcaggaagtt ttcgacgcgt tcctgtgtgg cgtgcggatt gaacttgcgg aaatagtgca
      721 atacggcggg aatgatgcct gccgcgccgt tggtcggtgc ggtaacgacg cgtccgccgg
      781 cggcgttttc ttcgttgacc gccatggcgt acaccatcgg ccagagctgg gtgttgacga
      841 tttcggtttc gcgcaggact ttgagcttgg cggcaagctg cggggcgcgg cggcggacgt
      901 tcaatcagcc gggcagttcg ccgtccgcac ccaagccgcg tttgatgcag ccttccataa
      961 cctcggcaac ggcagcggcg cggcggcgga tttcggcttc gccgcatccg gcaagcgcgg
     1021 cttcgtttgc caacacgact tcggagatgt cgagccggtt cagacggcat cgggcaagca
     1081 gttcggcgca actggtatag ggatagggaa cggctttttc cgtttccgcc tgccggtcaa
     1141 aatcttcttc ggtaacgaca aagccgccgc cgaccgaata ataaacctgt tcattcaata
     1201 ccgtgccgtc tgaagcatag gcggtaaaac gcaggctgtt ggggtgtttg ggcagcactt
     1261 gattgccgag tatgttcagg tcgcggtcgg ggatgaagcg gatttcttgc ccgttgagcc
     1321 ggaggatgtg ctgcgtgcgg atgcgttcga ggcgttcggg aatgccggca agcgggatgt
     1381 cgtgcggcag gctgccttcc aaaccgagca tcagcgcgtc aaatgtaccg tgtccgtatc
     1441 cggtcagtgc gagcgagccg taaatgtcga tgacgatgcg aacagcctgt gcatccaaac
     1501 ctgccgcaaa ggcggcggct gccttcatcg ggccgaccgt atgcgaacta gaagggccga
     1561 taccgatttt gaa
//
LOCUS       DAWGJV010000224         1568 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWGJV010000224 DAWGJV010000000
VERSION     DAWGJV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.224"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..>1568
                     /locus_tag="WHQ94_002011"
     CDS             <1..>1568
                     /locus_tag="WHQ94_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ2129234.1"
                     /translation="APGGYIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQV
                     QLAYDKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTA
                     AGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKEL
                     GKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASV
                     HTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVA
                     NKGKCRDGAIGAAVGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSVVALVKGDV
                     NTAANAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGI
                     AILRTPGEPWTRHDVQTYRQYYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYN
                     ELYVANPKSATGYFRVDSYNPATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAV
                     ISNVPSTPTTIRGR"
BASE COUNT          477 a          400 c          403 g          288 t
ORIGIN      
        1 ccgcacccgg cggctatatc gccgacatcc ccaaaggcaa cctcaaaacc gaaatcgaaa
       61 aactcgccaa acagcccgag tatgcttatt taaaacagct tcaagttgcg aaaaacatca
      121 actggaatca ggtgcagctt gcttacgata aatgggacta caaacaggag ggcatgacac
      181 ccgcagcagc agctgtcgtc gttatcgtcg taactgtatt gacctacggc gcactgtccg
      241 ccccggcagc cgccggaacg gcgggcgcgg caggcgcagg agcgggagga gccgcagcag
      301 gaacggcagc cggaactgga gtagcagcag gaacggcagc cacaaccgga gtagcagcag
      361 gcacatcagc tgcagctatc accacagccg caggcaaagc cgcactggcc agtctcgcca
      421 gccaagccgc agtttccctc atcaacaaca aaggagacat aaaccatacc ctgaaagaac
      481 tgggcaaaag cagcaccgtc agacaggccg ccaccgccgc cgtaaccgca ggcgtactgc
      541 agggcataag cgggctgaac acccaagcag ccgaagccgt cagcaaacat tttcacagtc
      601 ccgcagcagg caaactgacc gctaacctga tcaacagcac cgctgccgca agtgtccata
      661 ccgccatcaa cggcggcagc ctaaaagaca acttgggcga tgccgcactg ggtgcgatag
      721 tcagtaccgt acacggagaa gtagcgagca aaatcaaatt taatctcagc gaagactaca
      781 ttgcccacaa gatagcccat gccgtagcag gctgtgcatc ggcggtagca aataaaggca
      841 aatgtcggga cggcgcaatc ggcgcggcag tcggcgagat ggtgggagaa actctgttgg
      901 acggacgcga tgtaggcaaa ctgtcacccc aagaacgcca aaaagtcata gcctactcgc
      961 agattatcgc aggcagcgta gtggcattgg ttaaagggga tgtgaatacg gcggcgaatg
     1021 cggctactgt ggcagtggag aataatagtc ttttagctcg caggagggta aatatacgtt
     1081 ggactccgcg acaagaattg gaacatgaat atgccattct tgaaatccag gccattacca
     1141 atcaaatccg aaggctggat ccgaaattta acgggattgc tattctgagg actcctggag
     1201 agccgtggac aagacatgat gtacaaacat acaggcaata ttataatcaa ttaagggaat
     1261 ccagaggctt tgctgttgac ccaatttata gaatcaggat aaacaacggc aatgaattta
     1321 accgtatcat gtcatcaaaa tacccttata atgagcttta tgtagccaat cctaaatcgg
     1381 cgacggggta ttttagggta gattcgtata atcctgcgac agaggaaatt atttcaagaa
     1441 aatttaccca attttctcaa atccaagaaa gtacggggat tggttatatc aaggaggctg
     1501 ttagaaaata tagccctggt gctgtcattt ccaatgttcc aagtacacct actacgataa
     1561 gaggaaga
//
LOCUS       DAWGJV010000225         1500 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWGJV010000225 DAWGJV010000000
VERSION     DAWGJV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.225"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            33..1427
                     /locus_tag="WHQ94_002012"
     CDS             33..1427
                     /locus_tag="WHQ94_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ2129235.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIPKKAQMLQATPSNPTAAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWNSNM
                     LAPLGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          398 a          495 c          342 g          265 t
ORIGIN      
        1 tatccaccgt ccgccttttt acggaaaccg aaatgacccc ttccgcactg aaaaaaaccg
       61 tcctgctgct cggcactgcc tttgccgccg catccgcaca agcctccggc taccacttcg
      121 gcacacagtc ggtcaacgcg caaagcacgg cgaatgccgc cgccgcagaa gccgccgacg
      181 catcgaccat cttctacaac cccgccggcc tgaccaaact cgacagcagc cagatttccg
      241 tcaacgccaa tatcgtattt ccaagcatcc gttatgaagc ggattccgcc accgacttta
      301 ccggcattcc cgtccaaggt tcgaaaagcg gcaaaatcac caaaaccacg gttgcgcccc
      361 acatctacgg cgcatacaaa gtcaacgaca atctgaccgt aggcttgggc gtgtacgtcc
      421 ccttcggctc tgccaccgaa tacgaaaaag attccgtatt gcgccacaac atcaacaaac
      481 tcggtctgac cagcatcgcc gtcgaacccg tcgccgcgtg gaaactcaac gaacgccatt
      541 ccttcggcgc aggcatcatc gcccaacata cttctgccga gctgcgcaaa tatgccgact
      601 gggggatccc aaaaaaagcg caaatgctgc aagcaacacc ttctaatcct actgccgctg
      661 ctcaaatcaa agccgacgga cacgccgatg tcaaaggcag cgattggggc ttcggctacc
      721 aactggcgtg gatgtgggac atcaacgacc gcgcacgcgt gggcgtgaac taccgttcca
      781 aagtctcaca cacgctcaaa ggcgatgccg aatgggcggc agacgacgca atggcgaaac
      841 aactgtggaa tagcaatatg ctcgcaccgc tcggctacac ggcgaatgaa aaagcccgcg
      901 ttaaaatcgt tacgcctgag tctttgtccg tacacggtat gtacaaagtg tccgataaag
      961 ccgacctgtt cggcgacgta acttggacgc gccacagccg cttcgataag gcggaactgg
     1021 tttttgaaaa agaaaaaacc atcgtcaacg gcaaatccga ccgcaccacc atcaccccca
     1081 actggcgcaa cacctacaaa gtcggcttcg gcggttctta tcaaatcagc gaaccgctgc
     1141 aactgcgcgc cggcatcgct tttgacaaat cgcccgtccg caacgccgac taccgcatga
     1201 acagcctgcc cgacggcaac cgcatctggt tctccgccgg catgaaatac catatcggca
     1261 aaaaccacgt cgtcgatgcc gcctacaccc acatccacat caacgacacc agctaccgca
     1321 cggcgaaggc aagcggcaac gatgtggaca gcaaaggcgc gtcttccgca cgtttcaaaa
     1381 accacgccga catcatcggc ctgcaataca cctacaaatt caaataaacg gcccgccgtt
     1441 tgattgtaga aatgccgtct gaaacagatt tccgtttcag acggcattgt tttaggctcg
//
LOCUS       DAWGJV010000226         1489 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWGJV010000226 DAWGJV010000000
VERSION     DAWGJV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.226"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..217)
                     /locus_tag="WHQ94_002013"
     CDS             complement(<1..217)
                     /locus_tag="WHQ94_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ2129236.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWK"
     gene            1207..1458
                     /locus_tag="WHQ94_002014"
     CDS             1207..1458
                     /locus_tag="WHQ94_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
                     /protein_id="HEZ2129237.1"
                     /translation="MYIPLPTVIAIQSNIMAIVSTPHPALSALIILAHYHGIAAKSLG
                     LKAKVVRQPVKRLAMVTLPALVWCDDGNHFKEKWLFSAF"
BASE COUNT          505 a          276 c          212 g          496 t
ORIGIN      
        1 tcttccaatt actgcttaaa aagctatctg cagcttgaat agcattatta acagtaattt
       61 ccattttatt tctattgtta tccacattat agattaaatc agataaggat tgaatagaac
      121 caataaatga atcccaagct ttatatccca agagtggtga atagggtgtt aatttcacat
      181 ctccaaaacc gttaatatta aaatttgcat atgtcatatc tgctttcctt tcaagtaaat
      241 taaaaaaata acgtgaggaa ttaaaattaa aaataaatta attatcgact caatataata
      301 tgaatcaatt agctctctta tggtaaatat aagaggtaaa aatactaaaa ataaactaat
      361 aaagttcaga aaaatagtaa atggaatatt ttcatatgga aaaattctac aaaatacaag
      421 taatattcca tgacatatta gcaaatgagt aaaatttatt ataaaatata taaatatttt
      481 cccatcgtaa gatatattca aataaagaaa tataaacaat atgaagtata taaaatatat
      541 tttttggcat ctaaaaaata aacttttagc aatcatatat aaatagcttg tagaataaaa
      601 aacgaaaatt attaattatc gtaggtgtgc atttattcca acacggtttc atgcagctta
      661 aaaaccaaaa attctttctc cctttatcct ttcttaaaaa tagcgttttc aaattctgca
      721 acgacaggtt tcattcaagt aggactgccg tgattgcatc aattgcttcg cggcatggca
      781 tccatatcac acaacaggac atttattctc gggtaagtcc accttcatcc tcttccgcca
      841 gcaggaacag ttacaggcta cctaaaatat gctctgtgct cggatagtaa atatttcctt
      901 tctcactatt gtttgtcatt taatcgacag tctactcttt ctctaaaatt tcgtcaataa
      961 tagttaaata tatttaaata catttaaaat aataatatac acctgtttca gtctgtctaa
     1021 aacacgggaa aatatgttat ttgatcacaa cggtgacttt gggcagcctg taccttcaat
     1081 aacgcgtgga aaatagatat atacttatat cgtattcgag gctgtcttgt ttccctcaaa
     1141 caaccattct tccacacttt tgctttacaa ttttcgtgtg tatccttaca attaccccta
     1201 ttgtttttgt atattccttt gccaaccgtt attgctatcc aatctaatat tatggctatc
     1261 gtatctacac cacaccccgc tctttccgcc ctcatcatcc tcgcccatta tcacggcatt
     1321 gccgccaaat ctttgggatt gaaggcaaag gtagtacgcc agcctgtcaa acgtttggct
     1381 atggtgacct tacctgcatt ggtatggtgt gatgacggca accattttaa ggaaaagtgg
     1441 ttgttttcgg cattctgaaa cgcaacggac gggtctatac cgttgtggt
//
LOCUS       DAWGJV010000227         1441 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWGJV010000227 DAWGJV010000000
VERSION     DAWGJV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.227"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(80..1417)
                     /locus_tag="WHQ94_002015"
     CDS             complement(80..1417)
                     /locus_tag="WHQ94_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2129238.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGSGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLGIL"
BASE COUNT          448 a          381 c          350 g          262 t
ORIGIN      
        1 tgaacgaaat aggctgaacc tgaagcaaaa atgccgtctg aaaggctttc agacggcatt
       61 tttgccgttt ggagggcttt taaaggatgc ccaaagaatt gatgaaaaca ataataatgg
      121 caatcggggc aaggtagcgc aaggtattca gccacagcag cagcactgcc cgtggtacgg
      181 tgctgcccga tccggcatct tttaacacag actgcttgtc ctgaatccag gcggtaaaga
      241 tggaaacact caaagcacca atcggcataa tgacggcgga aataacatag tcccacaaat
      301 caaaaatggt tttgccgaaa accttaaact caccccatac gccgaaagac agcgcggacg
      361 ggatgccgac aatgaaaata gccgtgccga taagccaagt gtgctttttg cgtttgcgct
      421 cgtcttggcg gatggttgag gcaatgaccg tttccaacat cgaaaatgcg gaagtcagcg
      481 tggcgaaaac gaccagaagc atgaataccg caaacaaaac cgtaccgaac ggcatcttca
      541 taaacactgc gggcaatacg ataaaaatca atcccggccc ctggctcggt tcaaaaccga
      601 aggcgaacac cgccggaaaa atcaccaggc cggcaagcag cgaaaccaag aggttcatcc
      661 acataatcgt atggccggaa cggaacatat cctgatcttt tcccaaatat gaagcgtagg
      721 taatcatggc ggaaacgccg atgctcaggg caaaaaacgc ctgtcccaaa gccgtaatca
      781 tcgtatcggc tttaaagtac gaccaattcg gtttgagcag gaaagacacg ccctccattg
      841 cacccggcag cgtcagcgaa cggattgcca gcgcaataaa gaggataaac agccccggca
      901 tcagataacg gtttgccttt tcaatgccgt ctgaaatccc gcctttgacc acccaaaccg
      961 taatcagcat aaacagtgcc tgataggaca gcgaacctgc cggattggaa atcgttgtgc
     1021 cgaacagggc ttcaaagtcc gctcctgcat ggattgcccc tgtaaaactg tggacgacat
     1081 aattcaatac ccatccgccg accacgctgt aaaacgacag caaaataaag caggcggcaa
     1141 cgcccatgcg cccgacccaa agccattgcg tgcccggacg cagagccctg aaggaatcga
     1201 cggcattttt accgcccgtg cgcccgatat aaaactcggc aagctggacg ggcagggcga
     1261 ccaagatagt aaatatcaaa aacagcagga aaaacaccgc gccgccgttg gtgcctgccg
     1321 tataagggaa tttccaaatc gcgcccaaac cgatggccga acctgccgca gcaaggacga
     1381 aaccgatttt ggacgaccaa gaagtatggt tactcataaa atttattaag aaatgtgaaa
     1441 g
//
LOCUS       DAWGJV010000228         1427 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWGJV010000228 DAWGJV010000000
VERSION     DAWGJV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.228"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            128..1257
                     /locus_tag="WHQ94_002016"
                     /pseudo
     CDS             128..1257
                     /locus_tag="WHQ94_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          383 a          343 c          331 g          370 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgataat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttccga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 cttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgcgggggg ggcgatttgg aacgccttgc cgcccgcgat
      901 attatccgtt tccccggttt tgtgaacaat gtttccgaag tgataaaaga acatcatata
      961 ttcgtattgc cgtcttatta tagggaaggc gttccccgaa gcactcagga ggcaatggcc
     1021 gtcggcaggg cagtgattac gacggatgtc cccggatgca gggaaacggt cgccgacaag
     1081 gtcaacggct tcctgatcga gccttggaat ccccgcatct tggccgaaaa aatgatttat
     1141 tttatcgaaa acagggaagc cgtccgcctg atggggaatg caagttatgc gattgccaaa
     1201 gataaattcg atgccgaaaa agtcgatttg aaattgctcg atattttgaa ggcgtaaaca
     1261 aggctgcccg cttttgagtt tcggtcattt ctgataaacc cccgtcattc ccgccacacc
     1321 cccgtcattc cctcgaaagc gggaattcag gttcgtttag tttcggtcat ttccgataaa
     1381 ttcttgcaac tttgcgtttc tagattccca ctttcgtggg aatgacg
//
LOCUS       DAWGJV010000229         1402 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWGJV010000229 DAWGJV010000000
VERSION     DAWGJV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.229"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(41..1033)
                     /gene="porB"
                     /locus_tag="WHQ94_002017"
     CDS             complement(41..1033)
                     /gene="porB"
                     /locus_tag="WHQ94_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ2129239.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFD
                     DADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          319 a          322 c          366 g          395 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgat acgaatttgt tttcgccttt gccttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaatcgtt gcttaagtct gcatcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaata attgtcttca accaatttgg catcttgttg ctgtacggct acggaagcgt
      361 gcagggcatc attgtcgtaa ccgctgacca aacggtgaat ctggtatttt tcaatcttca
      421 cgtcatccac atcctgatgt cttttatagg caccgccata ttgcacgaag aagccgccgt
      481 ttttgtagtt gaagccggcg tggtaagatt cgctgttatg tctgcctgca ttgtcgttaa
      541 gcgcgtattg tacgctgccg ctgaggccgg caaattcggg agaatcgtag cgtacggaaa
      601 tgaggcgtgc ttcgggttcg gcaattttgt ttacacccaa atagtcgctt ttgctatccc
      661 aaggattgat gtcgccggtg tctttcagga cgctgttcaa acggccgacg cgcaatttac
      721 cgaagccgcc tttcaaaccg atgaaggatt ggcggttgcc ccaaccggag tcagtgccgg
      781 cgatagatgc cttttgctca acctgccaaa tggctttcag accgttgccg aggtcttctt
      841 ggcctttgaa gccgattttc gaacccaaat caacgatgcc ggtaccggtt tcaacgctaa
      901 ccacctgacc tccattgtgc tctacagagc gggaagtttc tacgccggct ttgatggtgc
      961 cgtacagggt aacgtcagcc attgctgcaa caggaagggc tgccaaagtc agggcaatca
     1021 gggatttttt cattgctgta ttcctttttt ggttaagaaa tttaagcagg ccgggctttc
     1081 caagccgctt agctttgcat ttaccgccga cgttttcggc gattccatgt ctgaaactat
     1141 agatgttcct gtctgaaaaa acaaacttct gcctgttttt gtgtggaatt cgatgtgtat
     1201 tttgaagggc ggatagagat attgtgggta ttttttgttt tataacatat ggttatttaa
     1261 attttttaag atttgcattt ttacaacact tactcgggag ggtattggag ggcattgcaa
     1321 accgggggtt ataaagacgg caaaaaacgc gcacgtggtt tctttcggaa cgggcggatg
     1381 caaaaccgcc cctgcggcag ga
//
LOCUS       DAWGJV010000230         1353 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWGJV010000230 DAWGJV010000000
VERSION     DAWGJV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.230"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            203..1282
                     /gene="apbC"
                     /locus_tag="WHQ94_002018"
     CDS             203..1282
                     /gene="apbC"
                     /locus_tag="WHQ94_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ2129240.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSNGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT          340 a          452 c          318 g          243 t
ORIGIN      
        1 gtgcattata cagcaccatc ctgcctcgtc aagcaaattt tacaggctta attgcagacc
       61 actgtttgca cgggatattt tgacaacgga ttttcacaat ccgccgcata ccgtgtaaaa
      121 gttcgcacaa ggaaaggcaa accgcccgaa atcaatgtac actttccgcc cgtttcccaa
      181 actgcacaca gaaacacaca ttatgaacat acaaaacatc cgcaccctcc tcgacaccgt
      241 cgccgttccg aatacggcac gcacgctcgg cggcgaaaag gccgtccgtt cggtcgaaca
      301 gcgttcagac ggcatccata tcgccctgca tttcggcttc cccgtcgcgc acattgcctc
      361 agaaacagcc gaccgcatac aggaaatcct gatgccgctg acaggcgaca cacacatcca
      421 tctgtccatg gacactgaaa tcggcacaca caaagtccag cccggcgtta ccaccatcaa
      481 aggtgtgaaa aacatcattg ccgtcgcatc gggaaaaggc ggcgtgggca aatcaaccac
      541 cactgccaac cttgccgccg cgatggcgcg gatgggcgcg cgcgtcggcg tactcgatgc
      601 cgacctttac ggcccgagcc aaccgaccat gttgggtgtg gacgaccgca aacccgacca
      661 gaaaaaccaa aaactgattc ctgtcgaatc ttcaaacggc atacaggtca tgtctatcgg
      721 ctttctcgtc gataccgacc aagccgtcgt ctggcgcggg ccgatggtca gccaagcctt
      781 gcagcaactg atgtttcaaa gcgagtggga cgaagtggat tatcttttca tcgacctgcc
      841 ccccggcacg ggcgacatcc aactcacgct gtcccaacgc atccccgtaa ccggatcggt
      901 catcgtaacc acgccgcagg acatcgccct gatagacgcg cgcaaagccg tggatatgtt
      961 ccgcaaagtc aacattccca ttttgggcgt attggaaaat atgtccgtcc acatctgcac
     1021 caactgcgga cacagcgaag cactgttcgg cacggacggc ggcaaagatt tggcggcccg
     1081 cctcaacgtc cccctgctcg gacagcttcc cctaagcctg cccgtgcgcg aagccatgga
     1141 cggcggcaca ccggcgcaac tgttcgacga acaccccgcc atcgccaaaa tctacaccga
     1201 tgccgcattc caaatcgccc tgagcattgc cgacaaaggc aaagacttca gcagccggtt
     1261 ccctaaaatt gtcgtcgaat aaagccgcgc ccgaaaccgc aacagcaatg ccgtcccaag
     1321 ccccgcgcct gccgatatgc cgtcattccc gcg
//
LOCUS       DAWGJV010000231         1233 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWGJV010000231 DAWGJV010000000
VERSION     DAWGJV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.231"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            41..373
                     /locus_tag="WHQ94_002019"
     CDS             41..373
                     /locus_tag="WHQ94_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ2129241.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WHQ94_002020"
     CDS             705..1214
                     /locus_tag="WHQ94_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ2129242.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          330 a          231 c          293 g          379 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatatgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcaaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tag
//
LOCUS       DAWGJV010000232         1186 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWGJV010000232 DAWGJV010000000
VERSION     DAWGJV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.232"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(10..456)
                     /locus_tag="WHQ94_002021"
     CDS             complement(10..456)
                     /locus_tag="WHQ94_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129243.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNI"
BASE COUNT          428 a          216 c          121 g          421 t
ORIGIN      
        1 gtctagagtc tatatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa ttagaaaatc
      901 aaaaaaaacc attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa
      961 ataacattgc gaaaacatat gaaatcatca aaataccaaa tatatagtgg gaatgcatta
     1021 ttatttagct ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc
     1081 atttgcaatt gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct
     1141 tgcaagatgt gcacctgcac aaccacctaa tactaaacca ccacct
//
LOCUS       DAWGJV010000233         1123 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWGJV010000233 DAWGJV010000000
VERSION     DAWGJV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.233"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(231..1085)
                     /locus_tag="WHQ94_002022"
     CDS             complement(231..1085)
                     /locus_tag="WHQ94_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ2129244.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWNVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          311 a          337 c          294 g          181 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaagccc aaatacagca gcgacaatac catcccgacg
      481 ttccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cagcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tag
//
LOCUS       DAWGJV010000234         1110 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWGJV010000234 DAWGJV010000000
VERSION     DAWGJV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.234"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..242
                     /locus_tag="WHQ94_002023"
     CDS             <1..242
                     /locus_tag="WHQ94_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ2129245.1"
                     /translation="LYYIKKGENPAIITHRNNRINQTEEDNYSTGVGSCINGFTVRYY
                     PFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            259..>1110
                     /locus_tag="WHQ94_002024"
     CDS             259..>1110
                     /locus_tag="WHQ94_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2129246.1"
                     /translation="MNEGEVVLTPEQIQTLYGYASRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSESDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYH"
BASE COUNT          375 a          157 c          256 g          322 t
ORIGIN      
        1 aactttacta tattaaaaaa ggagaaaatc cggcgattat tactcatcgg aataatcgaa
       61 taaaccaaac tgaagaagat aattatagca ctggcgtagg ttcctgtatt aacggtttca
      121 cggtacggta ttacccgttt attcgggaaa agcagcagct cacacagcag gagttggtag
      181 gttatcacca acaagtagag caattggtac agagttttgt aaacaatcca agtaaaaaat
      241 aatttaaagg atcttattat gaatgagggt gaagttgttt taacaccaga acaaatccaa
      301 accttgtatg gttatgcttc tcgtggcgat acctatggcg gttggcgtta tttggctaat
      361 ttgggtgacc gttatgcgga taatgctgcc gcaattgtcg gtaaggatgc aaacttaaat
      421 ggtttgaatt tatggatgaa aaaaggggtg gaaaacctat gggatgatac ggtcggtaaa
      481 aagacccgtt tagagaaatt tgatcgggtt gcattgcaac atttcagcca atatgtagat
      541 ctaattaatg aaaataatgg tagattacct aacactagtg aaattgagag aagttactat
      601 aaagccgtta cctatcatgg cgtttcttct agtgcagcta ttgatttagt tattaatcgt
      661 tcacttccgg atatggcgga tggttattgg gcattaggtt tggggataga agccgaacgt
      721 atccacaatg agcaagcagt aaataatccg aacggtagcg aatcggataa tagaaagcag
      781 ttaatatctg ctttagataa aggatttgat ggatctttta aagagaagca ttttactttt
      841 ttacaatctg tgataatgga tgtaacaaag ttaggtgttg aatatacaat agatggttgg
      901 caaaaaattg gaggttgggg taatgggata atcaatgatt tatataaaag tgttgcaaaa
      961 agagagtgga ctggaatatt tgagatcgtt aataataaca tcaagcaatt tagagatctg
     1021 ttcccaaatc cggaaggctg gatcgatgat ggtcaccaat gtttcgctcc ttgggttaaa
     1081 gaaactaaaa aacgcaatgg caaatatcat
//
LOCUS       DAWGJV010000235         1050 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWGJV010000235 DAWGJV010000000
VERSION     DAWGJV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.235"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            247..417
                     /locus_tag="WHQ94_002025"
     CDS             247..417
                     /locus_tag="WHQ94_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ2129247.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          317 a          289 c          228 g          216 t
ORIGIN      
        1 taacgcaaca ggaattcatc gaaaaaaccg aaacctccaa accgtcattc ccacggaagt
       61 gggaatccag taatcgaaaa accacaggaa tctatcgaaa aaaaccccaa cccccaaaaa
      121 aaccgggcgg ataccgcacc atcctcccaa accctgattt atagtggatg aacaaaaatc
      181 aggacaaggc gaggcaacgc cgtaccggtt tttgttcatc cgctatacaa aaacaaggaa
      241 acacaaatgg cgcaatatat gtgcggcccc tgcggctgga tttacgatga agaacacggc
      301 gaccccgaac acggcatccc ccccgggaca aagtttgaag acattcccga cgactggaaa
      361 tgccccgaat gcggcgtagg taaagaagat ttctacctgt tggatttcgt gatatagcgg
      421 cagccgaacc ccttcggaaa attcctcggc gttccgcctg cggtagcagg gaacaccgtt
      481 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
      541 cggaaccgat tcacttgtta aagaatcgtt ctctttgagc taaggcgagg caacgccgta
      601 ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg cacagatgcg
      661 acacggcttt tttctttctg ttgtcagaat gtaaattttc gtcaaaaaac atcaaaaatc
      721 cccccccccc ccccgaattt gcgtaacccg aatttgcgta aaataaagtg taatcccttt
      781 gatcataagg gaatgttgac aattagatcg attctcctag gctgactttc gcaataaatg
      841 gggttgtcaa taattgtgta tggtcgtttg ccgtccggcg gccgtcattc ccgcgaacgc
      901 aggaatccag caacacaatg cagcaggact ttatcggaaa aaaccgaacc cccaccgccg
      961 tcattcccgc gcaggcggga atccaaagac gcaacgcaac aggaatttat cggaaaaaac
     1021 agaacccccc cccaccgccg tcattcccgc
//
LOCUS       DAWGJV010000236         1035 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWGJV010000236 DAWGJV010000000
VERSION     DAWGJV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.236"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(302..688)
                     /locus_tag="WHQ94_002026"
     CDS             complement(302..688)
                     /locus_tag="WHQ94_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ2129248.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(685..930)
                     /locus_tag="WHQ94_002027"
     CDS             complement(685..930)
                     /locus_tag="WHQ94_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ2129249.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          281 a          244 c          184 g          326 t
ORIGIN      
        1 acacagccat atatattaag tacctgctct ttatgcgctt ttttaggaaa ggaagatcaa
       61 acggttcaat agcgaagtct tgcccaaatt tgatggacag gatatagaat acatgcgtgc
      121 ataccacacc ctgccaaagt tgggagatgc acttaaggtt gcacttgtgg tcgccaacta
      181 tgcctataca cgcggatttc ccgcgtttat aggccgcgac ggaagtctgg cttttaagcg
      241 aagacaataa ccgaaaagac cgcctcggcg gtctttcttg tgtacacagt ttttatattt
      301 gtcagcttga tgcattgaca actctaattc catattgcgg aatatattca tcgacagtca
      361 tcagttcaaa gccttccgct tgggtttgtg caatcaacat cctatcgaaa gggtctttgt
      421 gtatttccgg aaggcttcca gcctgttttg catgaaacag acctataggc agcatttcaa
      481 aatcctcttc ttgaagcaca tcaaaaaact cttccggtaa tttcaacaac cccttgttct
      541 gcttgatgga aatttcccaa atacttgctg cactgacaaa gatcgcattt ctcggatttt
      601 ctatcagttt gcgtgcagat gtccccagtt tcttgtcatc caacaaccac cacagcaaag
      661 catgggtatc aagcagaatc tttctcacag agccgactcc tcaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtatttccc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accggttagt tggacgcaag gcttacctgc
      841 tttcgcaata ataacgattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAWGJV010000237          969 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWGJV010000237 DAWGJV010000000
VERSION     DAWGJV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.237"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            97..>969
                     /locus_tag="WHQ94_002028"
     CDS             97..>969
                     /locus_tag="WHQ94_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2129250.1"
                     /translation="MNSQKLPEVKWGQSYNSLPQKSNPRQVNFTTYFSGTKKNTIFSF
                     NNTDEVVAQKNGTVVFGTATYLPPYGKVSGFDEKRLQERGDVLGWIGTTHPGLVGYSY
                     EGNTCSSGDCPDVSYKTQFTFGNQGLKNTVNGKLDIYEDKSRDNSPIYKLPDQPWLGV
                     SFNLSSESVVESKKLKKVESSFNEDVTQNNGAQSPYKDTNLVYTTGDGRNKGNQVHQD
                     KHHAVVFYLNAKLHLLDKKQIKDIMRGSELNLGDLKTRIEPTDAWKNKRHRTLTVGQW
                     EFKDTGKISVKLKLP"
BASE COUNT          295 a          225 c          233 g          216 t
ORIGIN      
        1 ggttttccgc cataccgcgc tttatgccgc catcttgatg ttttcccata ccggcggggg
       61 ggggcgcaag ccgacaccaa gccatacgct attgtaatga acagtcaaaa gctgcccgag
      121 gtaaagtggg ggcaatcata taattcattg ccgcaaaaaa gcaacccccg ccaagttaac
      181 tttacgacat attttagcgg aaccaaaaaa aacactattt tctcattcaa taataccgat
      241 gaagttgttg ctcaaaaaaa cggtactgtc gttttcggca cggcgaccta cctgccgccc
      301 tacggcaagg tctccggttt tgatgaaaag aggctgcaag agcgcggcga tgtccttggt
      361 tggattggta cgacccaccc ggggttggta ggctacagct acgaaggtaa cacatgctcc
      421 agcggagact gtcctgatgt tagctataaa acccaattta ccttcggtaa tcaagggttg
      481 aaaaacacgg taaacggcaa gctggatata tacgaagaca aaagccgcga caattcgccc
      541 atttataaat tgccggatca gccttggttg ggcgtgtctt tcaatttaag cagcgagagc
      601 gtcgtcgaat caaagaaact caagaaagtg gaatcttctt ttaacgagga tgtgacgcag
      661 aacaatggtg cgcaaagccc atacaaagac acaaacctcg tttatacgac aggcgatggt
      721 cgtaataagg gtaatcaagt ccatcaggac aaacaccacg ccgtcgtctt ttatctgaac
      781 gccaaactgc acctgctgga taaaaaacaa atcaaagata tcatgcgagg cagtgaactt
      841 aatttgggtg atttgaaaac gcgtatcgag ccgacggacg cgtggaagaa taagaggcat
      901 cgcactttaa ctgttggtca gtgggagttc aaagataccg gaaaaatcag cgtcaaactc
      961 aaattgccg
//
LOCUS       DAWGJV010000238          955 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWGJV010000238 DAWGJV010000000
VERSION     DAWGJV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.238"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..866)
                     /gene="mafB"
                     /locus_tag="WHQ94_002029"
     CDS             complement(<1..866)
                     /gene="mafB"
                     /locus_tag="WHQ94_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2129251.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQ"
BASE COUNT          228 a          236 c          210 g          281 t
ORIGIN      
        1 tcttgtaatg cggttgcggc cgcaagttgt gcttccggac ttaaatttcc taaattgtga
       61 ataccctgta aagttttccg tgctgccgca taggttaccg ggtttaccgc actgtctgta
      121 attgccccaa ctcccaatcc ctgaaaaccc attaaaaaag gattaaccgc accttggacg
      181 atgccgcgaa tatcatccat acggttagcc caccaatttt tattggggtc gctttcccat
      241 atcagttttc ctgcttcatc cgcacggctg aaaaaaccag aggcggcacc ggcattttct
      301 tttagccaac ggtctgaaaa tggggcttga gggacaatat tagtctttgt ttttgttgaa
      361 gttcctttga cataatagct gtatatatcc cttgctcctc cggggggcgg ataatcgctg
      421 ccttgcggcc cgtcatatcc atcctccgga tgaatttccg accctgtccg atgaagttgg
      481 taaacagtaa aaccaccgtc tacaccgccg ctgaaatcag aagtgctttt tgaatcatga
      541 ttatcgaacg gactgtgtac ttcatgtcca tgtcctgaaa aatgggtttc ataaccgata
      601 atgccttcaa accctgtccg ttcgtgtgta ataggcagta tggggccgac cgcagttgca
      661 tcaaatgtct ggacggcgta aacccgattt ttaacactgc cgcgagcatt accaaacaga
      721 tggtatttac cacccggttc gtagtgtttt gcctgcatat catcgcgtaa acgggcatcc
      781 aaaccgttcg catgactaat ggggatttgc agcaacgata ttgccgctgc aaacagcatc
      841 atgaattttt gaataggcaa tttcattttg ttttccttgg tagcaagcgg ttatggtcgt
      901 gtgaatcaag gttgcccttg tctatgttgt cgcactactt catcgctgta tccat
//
LOCUS       DAWGJV010000239          922 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWGJV010000239 DAWGJV010000000
VERSION     DAWGJV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.239"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..>922
                     /locus_tag="WHQ94_002030"
     CDS             <1..>922
                     /locus_tag="WHQ94_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2129252.1"
                     /translation="VFRHTALYAAILMFSHTGGGAQAQTNKYAIIMNEGNQLEVKPNG
                     QYSTIKNKDREREYTHHQYTGGGSVSFNNSDELVSRQSGTAVFGTATYLPPYGKVSGF
                     DADGLQKRNNAAGWIRTTRIALAGYSYADVVCRSNAGCPKLVYKTQFSFDNPDLAKTG
                     GVLDRHTEPSRDNSPIYKLKDYPWLGVSFNLGAEGTAKNGKVTNKLVSSFDEKNSNNN
                     LVYTTEGSDISLGNRHRETTAMAYYLNAKLHLLDKKQIQNITDKTVRLGVLRPSIDVR
                     QGNTGIAGLLTFWARWDIKDTGQIPVKLSLT"
BASE COUNT          258 a          232 c          232 g          200 t
ORIGIN      
        1 ggttttccgc cataccgcgc tttatgccgc catcttgatg ttttcccata ccggcggggg
       61 ggcgcaggcg caaaccaata aatacgctat tatcatgaac gagggaaacc agctcgaggt
      121 aaagccgaat gggcaatatt caacaataaa gaacaaagac agggagcgcg aatatactca
      181 tcatcagtac acaggaggag gctctgtctc attcaacaat agcgatgagc ttgtttctcg
      241 acaaagcggt actgccgttt ttggcacagc cacctacctg ccgccctacg gcaaggtttc
      301 cggttttgat gccgacgggc tgcaaaagcg caacaatgcc gccggttgga ttcgtaccac
      361 ccgcatcgcg ctggcaggct acagctacgc cgatgtcgta tgcagaagca acgcaggctg
      421 tcccaaactt gtctataaaa cccaattttc cttcgataat cccgacttgg caaaaacagg
      481 aggcgtgctg gataggcaca cagagccaag ccgcgacaat tcgcccattt acaaattgaa
      541 agattatcca tggttgggcg tgtctttcaa tttgggcgcc gagggtaccg ccaaaaatgg
      601 taaggtaacc aacaaattgg tatcttcttt tgatgaaaag aatagtaata acaacctcgt
      661 ctataccacg gaaggcagcg acatttcctt gggcaacagg catcgcgaaa cgaccgccat
      721 ggcctattat ctgaacgcca agctgcacct gctggataaa aaacagattc aaaatatcac
      781 cgacaaaaca gtgcggttgg gtgtcttgag gccgagcatc gatgtgcggc aaggaaatac
      841 ggggattgcc ggcttgctaa ctttttgggc taggtgggac attaaagata ccgggcagat
      901 tccagtcaag ctcagcttga cg
//
LOCUS       DAWGJV010000240          906 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWGJV010000240 DAWGJV010000000
VERSION     DAWGJV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.240"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(55..834)
                     /locus_tag="WHQ94_002031"
     CDS             complement(55..834)
                     /locus_tag="WHQ94_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ2129253.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          222 a          291 c          179 g          214 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcaaccaaa acattcaatg ccgaccggac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaagg
//
LOCUS       DAWGJV010000241          905 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWGJV010000241 DAWGJV010000000
VERSION     DAWGJV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.241"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..215
                     /locus_tag="WHQ94_002032"
     CDS             <1..215
                     /locus_tag="WHQ94_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129254.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTRATLLALDWQGN
                     KPLGAAELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WHQ94_002033"
     CDS             546..>905
                     /locus_tag="WHQ94_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129255.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          280 a          148 c          212 g          265 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacacg tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggaactggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgctgtc gtgccaacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaagtgg cataaaaatc
      901 tatat
//
LOCUS       DAWGJV010000242          875 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWGJV010000242 DAWGJV010000000
VERSION     DAWGJV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.242"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            424..666
                     /locus_tag="WHQ94_002034"
     CDS             424..666
                     /locus_tag="WHQ94_002034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129256.1"
                     /translation="MLELSTYCVSPSAGSVVDFDLKKRGGVGQDVFRGTPDCGGVCLY
                     FAEGFKNRVVGYIGCTVLVCARSGQGAMGKFARDAV"
     gene            complement(686..>875)
                     /locus_tag="WHQ94_002035"
     CDS             complement(686..>875)
                     /locus_tag="WHQ94_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129257.1"
                     /translation="TIMRPFDGVKYKDTLTAGFSSPHHHLTADSYLQKRREPDRWYSR
                     IRRAVREAATSRAPSHDE"
BASE COUNT          166 a          168 c          312 g          229 t
ORIGIN      
        1 aacgaccgca gtgaaaaaat aggggaacct gtaagggaag gggcatcggc tgcggccggt
       61 gcttttttgt ttggaaggga aacggcagga aaaagacccg cgccatatcg gggaatgaac
      121 cgacgcaaag gaaatgcggt ttgaatatgc gtccgggcgg tgttttactg tgtgtgccgt
      181 atggcggggc agggtgtctg atttcgtgat gaccgtgttg gtggaatcga agcctgccgt
      241 tccggtaagg gttgcccggg agggcaaggc cggctttgta tgcccggtta agagggaagg
      301 atgagactgt gcatttttga ttttaattgg ctatatgccg tctgaaaatc ggtttgaagg
      361 cggtactttt atgctgcctt tcatacaggg gaatgggaac gttgcggctg cgcggaaacc
      421 gtggtgcttg aactttctac ttactgcgtt tcgccgtcgg cgggttcggt ggtggatttt
      481 gacctgaaaa aaaggggggg tgttgggcaa gatgttttta ggggaacgcc ggattgcggt
      541 ggggtttgcc tttattttgc agaaggcttt aaaaaccgtg tggtcgggta catcggctgt
      601 accgtgcttg tttgtgcaag atcggggcag ggtgcgatgg gcaagtttgc ccgggatgcc
      661 gtctgaagcg ttcagacggc attttttatt cgtcgtggct tggtgcgcgg gatgtggcgg
      721 cttcccggac ggcacggcgg atgcggctgt accatcggtc gggttctcgc cgcttttgga
      781 ggtaactgtc ggcggtcagg tggtggtgcg ggcttgaaaa accggcggtt agggtgtctt
      841 tatatttcac cccgtcaaaa gggcgcatta tagtg
//
LOCUS       DAWGJV010000243          843 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWGJV010000243 DAWGJV010000000
VERSION     DAWGJV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.243"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            704..>843
                     /gene="pilE"
                     /locus_tag="WHQ94_002036"
     CDS             704..>843
                     /gene="pilE"
                     /locus_tag="WHQ94_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ2129258.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLA"
BASE COUNT          287 a          223 c          134 g          199 t
ORIGIN      
        1 cagaaacata aaattcgtca ttcccgcaaa agcgggaatc tagttcgttg agtttcagtt
       61 atttagaata aattttgaaa cttaaatccc gtcattccca cgaaagtggg aatccagaac
      121 gcaaaatcta aagaaaccgt ttacccgata aatttccgta ccgacaaacc tggattcccg
      181 cctgcgcggg aatgacgaat ccatccgcac ggaaacctgc cgcgatgatt caactgccgt
      241 ttgcacagga acaccacgcg ccgatttcaa tcactttcca agaaaacgga aatttttaaa
      301 aattaaattt aaaatttccc acccaaccca ccctatgcta ccgcgcaaat tcaaaaatct
      361 caaattccga cccaatcaac acacccgatg ccccatgcca atagagatac cccacgccaa
      421 taaaaaagtg ccgaaaatcg gcactaaaac tgacaatttt cggcactgct gacaaaaaac
      481 ggtaatcccc tgcttcctca aaccacgccc aaacgaaaga aaatgtaaaa taaaaacaac
      541 catataaaga taaacgcata aattttcacc tcaaaacaca aacttgatac caatcttgct
      601 ttataataca caggtatcgc aacaaaatac cgatggtcaa atacattgca taatgccgat
      661 ggcgtaagct tgaggcattt cctttccaat taggagtaat tttatgaaca cccttcaaaa
      721 aggttttacc cttatcgagc tgatgattgt gattgccatc gtcggcattt tggcggcagt
      781 cgcccttcct gcttatcaag actacacagc ccgcgcacaa gtttccgaag ccattctttt
      841 ggc
//
LOCUS       DAWGJV010000244          838 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWGJV010000244 DAWGJV010000000
VERSION     DAWGJV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.244"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(110..394)
                     /locus_tag="WHQ94_002037"
     CDS             complement(110..394)
                     /locus_tag="WHQ94_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129259.1"
                     /translation="MKLALKRSGKYNVYDPLALDLDGDGIETVAAKGFAGALFDHRSQ
                     GIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(391..684)
                     /locus_tag="WHQ94_002038"
     CDS             complement(391..684)
                     /locus_tag="WHQ94_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ2129260.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
BASE COUNT          240 a          179 c          145 g          274 t
ORIGIN      
        1 ctatacgaat aaaaaaatat cctacctagc ccgaaccttc ttataatttg tactatgtga
       61 tcattcattt caatatccat ttaagctttc aattaatttt ctatattttt caatggcatg
      121 tatcatttgg ttgatgatca agccatgtat gcaaagattg gaatatggta tctgtcgtgc
      181 taataatgcc cccgttactg tttaatttgc gcacaggaaa accgtcatat gcagcaatcc
      241 agcccgtggc ggtgcggatg ccttggctgc ggtggtcgaa caatgcacct gcaaagcctt
      301 tggcggcgac agtttctatg ccgtcaccgt ccaaatctag ggcaaggggg tcgtaaacgt
      361 tatatttgcc tgaacgtttg agagctaatt tcaaccactc tcttgcccat tcagggaatt
      421 caggcagcca atcttcaaat ttcggaaatt ggtcccattc ttttcaagat aagggagaaa
      481 atcttgttta taccattttt ccagtaactc tccccattca cttatccctc gtccaatata
      541 atcttcgccc cctttccaag ggtttgcatc atcaggaact aaggaatata taaacctaga
      601 tacttcttct aatacattgc tgtatgtatc aggatccgca aaaaagtttg ctatcttact
      661 tggtgtaaaa tcatctatat tcattataga ttcgccaagc ctctcgtttc cataatcctt
      721 atttaattca attcatgctg taaaacagta gaggatttta gcatttttct cgtatatctc
      781 gagatacatt tcttaaattt aaaaaaatgt tatcgaggga tgcaagtaaa gaagcagt
//
LOCUS       DAWGJV010000245          829 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWGJV010000245 DAWGJV010000000
VERSION     DAWGJV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.245"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            44..>829
                     /locus_tag="WHQ94_002039"
     CDS             44..>829
                     /locus_tag="WHQ94_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ2129261.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFT"
BASE COUNT          269 a          240 c          184 g          136 t
ORIGIN      
        1 caagtaaata ttcagattcc ctatatactg cccagacgcg tgcgtgctga agacaccccc
       61 tacgcttgct gcagaacttt cgggtaaaac cggtgtgagc attagcgcac cgtatgccaa
      121 tgagaacagt cgcatcctgc tcagcaccac ggatatcagt tcggaaaacg gcaaaatcaa
      181 aattcaatct tacggtgacc aatattacta tgcgagacag agcgaactct atacctttga
      241 acgccgcagc tacaaaaccg gcaaatggta caaccgcaaa cacattaccg aagtcaaaga
      301 acacaaaaac gccaagcccg acgcagtaaa cctcagcgca tcccaaggca tcgacatcaa
      361 atctggtggc agcatcgacg cctacgccac cgcattcgat gcccccaaag gcagcattaa
      421 catcgaagcc gggcggaaat tgacactcta tgccgtagaa gagctcaact acgacaaact
      481 agacagccaa aaaaggcgca gatttctcgg catcagctac agcaaagcac acgacaccac
      541 cacccaagtc atgaaaaccg cgctgccctc aagggtagtt gcagaatctg ccaatctgca
      601 atcaggttgg gataccaaac tgcaaggcac acagtttgaa accacactgg gtggcgcaac
      661 catacgcgca ggcgtaggcg agcaggcacg ggcagatgcc aagattatcc tcgaaggcat
      721 caaaaccacg atccacaacg aaaccgtgag cagcagcaaa tctgctctat ggcaaaaaca
      781 ggcaggacgg ggcagtaaca tcgaaacctt gcaattgccg agtttcacc
//
LOCUS       DAWGJV010000246          813 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWGJV010000246 DAWGJV010000000
VERSION     DAWGJV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.246"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            262..>813
                     /locus_tag="WHQ94_002040"
     CDS             262..>813
                     /locus_tag="WHQ94_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ2129262.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHI"
BASE COUNT          276 a          146 c          149 g          242 t
ORIGIN      
        1 agtgccaatt ctatgaagca atacggtaag taacaatgaa aatatctact gcttgggtat
       61 agagcatatt tcacaacccg taactattct tgcggaaaca gagaaaaaag tttctcttct
      121 atcttggata aatatattta ccctcagttt agttaagtat tggaatttat acctaagtag
      181 taaaagttag taaattattt ttaactaaag agttagtatc taccataata tattctttaa
      241 ctaatttcta ggcttgaaat tatgagacca tatgctacta ccatttatca actttttatt
      301 ttgtttattg ggagtgtttt tactatgacc tcatgtgaac ctgtgaatga aaagacagat
      361 caaaaagcag taagtgcgca acaggctaaa gaacaaacca gtttcaacaa tcccgagcca
      421 atgacaggat ttgaacatac ggttacattt gattttcagg gcaccaaaat ggttatcccc
      481 tatggctatc ttgcacggta tacgcaagac aatgccacaa aatggctttc cgacacgccc
      541 gggcaggatg cttactccat taatttgata gagattagcg tctattacaa aaaaaccgac
      601 caaggctggg ttcttgagcc atacaaccag caaaacaaag cacactttat ccaatttcta
      661 cgcgatggtt tggatagcgt ggacgatatt gttatccgaa aagatgcgtg tagtttaagt
      721 acgactatgg gagaaagatt gcttacttac ggggttaaaa aaatgccatc tgcctatcct
      781 gaatatgaag cttatgaaga taaaagacat att
//
LOCUS       DAWGJV010000247          812 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWGJV010000247 DAWGJV010000000
VERSION     DAWGJV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.247"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..177)
                     /locus_tag="WHQ94_002041"
     CDS             complement(<1..177)
                     /locus_tag="WHQ94_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ2129263.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAA"
     gene            complement(299..688)
                     /locus_tag="WHQ94_002042"
     CDS             complement(299..688)
                     /locus_tag="WHQ94_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129264.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT          223 a          153 c          189 g          247 t
ORIGIN      
        1 ggcggctttg cgtcctttca gggcggacaa atccatttct ttgacggcgg cgcgggacga
       61 tgcggcgacg agttcttgtt cgacggcaaa gcgtttgccg ccgccgtggg cgggtatgcc
      121 ggtcagtgtg ccgcaggcgg tgaggacgag ggggatgagg aggagcaggg ttttcatggc
      181 agggtttgtt ttgttttgaa cgagtttgag tgtaaatgga ttttaagggt ttgtaaacaa
      241 aaggggcgaa aatgccgtct gagcggcgga aatggctttc agacggtatt ttgactgttt
      301 aaagcggggg cagttctaca aacggaaaga aatgctgaaa cctctgataa atttcaggat
      361 gtttctttaa ggcttttaat gctttttctt ttgaaatagg cggatcatag acatctatcc
      421 cccttaagaa ggcaatgcct gttaatgcgt catcttggtc tttagataag tctgaattcg
      481 gaacaaaatc caaaatatta taaatatctc ctactccttc tttaagatca attaaatcaa
      541 aaatataaaa atgtatatta tcaataggca tatctaagac aaccttctcc aaccataatt
      601 taaattcttg caaattaatt gctgccgata ataagcaata tgttgcaaaa cttaaatctt
      661 gaccttgctc tttcgtaatt ttccacataa ttatcttcct ttgccgtcaa acgctctttt
      721 ggttacctgc tttgtatcaa aaatgccgtc tgaaagccga atattgtttc agacggcatt
      781 ttgactgttt aaagcggagg cagctctaca aa
//
LOCUS       DAWGJV010000248          800 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWGJV010000248 DAWGJV010000000
VERSION     DAWGJV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.248"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..394)
                     /locus_tag="WHQ94_002043"
     CDS             complement(<1..394)
                     /locus_tag="WHQ94_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HEZ2129265.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARVAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDG"
BASE COUNT          288 a          110 c           97 g          305 t
ORIGIN      
        1 tgccgtcttt tttggtgtag ctgccttgtt gagccaaagt gttgccgtta ccaagatttt
       61 tgtttacatc ttttcgagaa aattggcttt gcatttgcct taacaaagat atttctgcta
      121 ctcgtgcttt attatttata ttatatttac tctgcatttt tcttacaact tgtctcaact
      181 gactttgtgt tttttgtaat tcacgttgta ccgattttcc taaactttca tgtttggctg
      241 cataattggt agaatcagtt gacatttgtg tataaaaatt tttacgaaaa ttttcgtaac
      301 tcagattaat tacatccgta cttgcagtta taaaatcttg tataaattca atagaaactg
      361 aattatcatc agatttggct ttgttatttt ccatattgag cctctcttaa tttgaaaaaa
      421 ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaaataa tgagaatata
      481 aaggataaaa agagagttaa gttatttaat cggacttgtt ctctataaat cataaggcac
      541 caaataagcc ataataaatt tagtaaataa aaactgctta tacccaactt tcttttcttg
      601 gaacctatta ttatttcaac tctggatgaa aaaatagaat ttaaaaattt ttttctttga
      661 tatatcgtta atatctccct agtttttctt aaaaaaaata taatcatata tatactacac
      721 aacattggta ataaataaat taaaagcgaa aggtaattaa attgataatt gtaaattata
      781 taataaacaa catacgaaga
//
LOCUS       DAWGJV010000249          798 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWGJV010000249 DAWGJV010000000
VERSION     DAWGJV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.249"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            337..636
                     /locus_tag="WHQ94_002044"
     CDS             337..636
                     /locus_tag="WHQ94_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ2129266.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            630..798
                     /locus_tag="WHQ94_002045"
                     /pseudo
     CDS             630..798
                     /locus_tag="WHQ94_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          216 a          219 c          172 g          191 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat tggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaac
//
LOCUS       DAWGJV010000250          761 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWGJV010000250 DAWGJV010000000
VERSION     DAWGJV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.250"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            1..648
                     /locus_tag="WHQ94_002046"
                     /pseudo
     CDS             1..648
                     /locus_tag="WHQ94_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT          214 a          212 c          209 g          126 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgacc ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgacttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtc ctgactttgg
      661 gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacgcgga acggcagccg
      721 gagctagcaa ggtagaagtg aaggcaaacg gaaatattga g
//
LOCUS       DAWGJV010000251          729 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWGJV010000251 DAWGJV010000000
VERSION     DAWGJV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.251"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..227
                     /locus_tag="WHQ94_002047"
     CDS             <1..227
                     /locus_tag="WHQ94_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ2129267.1"
                     /translation="AADSDDVAAANGKTADNINTKHLPSTCCDASSAVCIETPRADFK
                     HFQKISRHRVLPESRHMARRQQFCRRGNLL"
     gene            169..543
                     /locus_tag="WHQ94_002048"
     CDS             169..543
                     /locus_tag="WHQ94_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ2129268.1"
                     /translation="MNHGIWPGDNSSAGVATSSKIKGKYVKEVTVANGVVTATMLSSG
                     VNNEIKGRKLSLWAKRQDGSVKWFCGQPVERTAPTTTNDDVKADTDATNKIDTKHLPS
                     TCRDDSSVVCIETPPTAFYKNT"
     gene            653..729
                     /locus_tag="WHQ94_002049"
                     /pseudo
     CDS             653..729
                     /locus_tag="WHQ94_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          219 a          226 c          157 g          127 t
ORIGIN      
        1 ccgccgccga cagcgacgac gtcgccgccg ccaacggcaa gaccgccgac aacatcaaca
       61 ccaagcacct gccgtcaacc tgctgcgacg cttcatctgc cgtttgcata gaaacaccac
      121 gcgccgactt caaacacttc caaaaaatca gccgtcacag agtattacct gaatcacggc
      181 atatggcccg gcgacaacag ttctgccggc gtggcaacct cctctaaaat caaaggaaaa
      241 tatgttaagg aagttacagt cgcaaacggc gtcgttaccg ccacaatgct ttcaagcggc
      301 gtaaacaacg aaatcaaagg cagaaaactc tccctgtggg ccaagcgtca agacggttcg
      361 gtaaaatggt tctgcggaca gccggttgag cgcaccgccc ccaccacaac caacgacgac
      421 gtcaaagccg acaccgacgc caccaacaaa atcgacacca agcacctgcc gtcaacctgc
      481 cgcgatgatt catctgtcgt ttgcatagaa acaccaccta cggctttcta taaaaatacc
      541 taaaccgtca ttcccacgaa agtggggatc taggacgtaa aatctaaaga aaccgttttc
      601 ccgataagtt tccgtaccga caaggctgga ttcccgcctg cgcgggaatg acgaatccat
      661 ccttttggct gaaggtcaaa aatcagccgt caccgagtat tacctgaatc acggcaaatg
      721 gcagaaaaa
//
LOCUS       DAWGJV010000252          704 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWGJV010000252 DAWGJV010000000
VERSION     DAWGJV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.252"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..228
                     /locus_tag="WHQ94_002050"
     CDS             <1..228
                     /locus_tag="WHQ94_002050"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ2129269.1"
                     /translation="KKQTGSLKHQVTGLNAVKSDRQKPAQYVGQHQDAYLHEIAKHFD
                     CTAATVCYALKQMGITRKKRPPLTKNKTRPK"
     gene            237..>704
                     /locus_tag="WHQ94_002051"
     CDS             237..>704
                     /locus_tag="WHQ94_002051"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2129270.1"
                     /translation="MTQPAEFSDYQRVYLDETGFDRHLFRPYARSLKGQIVKAQISGK
                     RYRRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHR
                     MGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALL"
BASE COUNT          210 a          166 c          161 g          167 t
ORIGIN      
        1 aaaaaacaaa caggcagcct aaaacatcaa gttaccggtc taaatgccgt caaatcggat
       61 aggcaaaaac cggctcaata tgttgggcaa caccaggatg cctatctgca tgaaatcgcc
      121 aaacattttg attgtacggc agccaccgtt tgctatgcac tcaaacagat ggggataacg
      181 cgcaaaaaaa gaccaccact tacaaagaac aagacccggc caaagtaacg cattatttga
      241 cacagccggc cgaattttct gactaccaac gcgtttattt ggatgaaaca ggatttgacc
      301 gccacctgtt ccgtccctat gcccgcagcc tgaaagggca aatagtgaaa gcgcagataa
      361 gtggaaaaag ataccgacgc ttatctctgg tgtccgcaca agtcggcaac cggctgattg
      421 ctccgatggt ttatcaaaat acgatgaccg gagtcttttt tgaagcgtgg tttcagcaat
      481 gcctactgcc cgcattgact caaaaatcgg tgattatttt agataatgcg cgatttcacc
      541 gtatgggtgt cttacgggaa atggcggaaa aattgggaca taaggtattg cctcttgcac
      601 cttattcacc tgagctcaac ccgattgaga aagtgtgggc gaatattaag cggtatctgc
      661 gaaccgtttt gtctgattac gcccgatttg acgatgcact actg
//
LOCUS       DAWGJV010000253          692 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWGJV010000253 DAWGJV010000000
VERSION     DAWGJV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.253"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(10..576)
                     /locus_tag="WHQ94_002052"
     CDS             complement(10..576)
                     /locus_tag="WHQ94_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ2129271.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMLREGLSGGADDVREAKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          207 a          246 c          136 g          103 t
ORIGIN      
        1 tttccgacat cattgaatca aacccaaatg cgacaagagc gtccatgtgc cgatggcaat
       61 caacaccaaa cctccggcaa attccgcaca cctgccgaac aatacgccca aagcccttcc
      121 cgccgtcagc ccgaccgtaa ccatcaccgt cgtcgccatg ccgatgacgg cggcggcaaa
      181 ggcgatgttt acctccataa acgccaagcc caccccgact atcatggaat caatactggt
      241 tccaaaagca gtcagaaccg tcaaccacca gctttcccgt ttggcttcgc gcacatcgtc
      301 cgccccgccg gacaagccct cacgcaacat cttcagaccc agcccgccca gcaggacgaa
      361 agccgcccaa tggtcccatt cgctgataaa cggcttggca taaaaaccgc ccacccagcc
      421 tgccagcggc gtgagcgctt caaccgtgcc gaacaccaaa gccgttgccg caattttgcg
      481 cggaggcatt ctgaccgccg caccctttgc caatgcgacg gcaaacgcat ccatcgacat
      541 ccccagcgca atcaagagca aagcataaaa acccataccg cacccgtcct caaaaagggc
      601 ggattatagc aaaagcaaaa aaatgcaaaa acgccgcacg aaaacccgca tcccgtcatt
      661 cctgcaaaaa caaaaaatca aaaacagaaa tc
//
LOCUS       DAWGJV010000254          680 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWGJV010000254 DAWGJV010000000
VERSION     DAWGJV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.254"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(94..645)
                     /locus_tag="WHQ94_002053"
     CDS             complement(94..645)
                     /locus_tag="WHQ94_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129272.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVRPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
BASE COUNT          191 a          276 c          125 g           88 t
ORIGIN      
        1 aagcaaagaa accgccatta gcactacacc cccccaagct gcggctgcca aacacatcgg
       61 ctgcttctcg acgcacccgc tgaaaaatgc gccctatccg cccgccttga ccgcaccccc
      121 tgcctttgcc gccctgcccg atgccgtctg aagcctgaac cgccccacca gcccgatgac
      181 gcacgacacc gccggaaagg gcatacacaa agccgtcagc acataacgcg cattccccgt
      241 attaaccacg tccgcgccct tcagcagcag cggcagcgcg ttcgcccact cccacaccga
      301 cggcgcgctg aacgccaata ccgccaccag ccccaacatc cggaacaccc cgatttccaa
      361 cacctgccgc cgccgcaccg aacggttgag caaaaacaca accgccaacc cgaaacagac
      421 caccgccgcc ataccgccgt tggcgacaat ctgcaaactg ttgaatgcaa tatcgggcct
      481 aaccgccaac acgccttccg aatccggcac ggcacgctgc aaatccaaac cctgcaacac
      541 attcaacaca aaaccgtaga tcaaatcaaa aaacaccacg cctatcggca aagcactcaa
      601 aacccgccac atcttcctgt ccgaccaacc gttcaaaacc ttcattttac ccgaccacgc
      661 aagccgccga acaaaaaaca
//
LOCUS       DAWGJV010000255          670 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWGJV010000255 DAWGJV010000000
VERSION     DAWGJV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.255"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WHQ94_002054"
     CDS             complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WHQ94_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ2129273.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          198 a          214 c          120 g          138 t
ORIGIN      
        1 tgttaatcca ctatattttc aaaccggaaa aatgccgtct gaaccttcag acggcattcc
       61 attcacattt attcgtcttt tttgtgttcg atggcgtttc gggaacgatt gccgccgtaa
      121 ggctcttcaa ccgtatattc cccctcgata atatcgtcat cgcgggaaaa gccctctttt
      181 ctgcccgatt ggttcatgtt gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct
      241 cccttaaacg gcagcagcag caataccgcc aacaccgagg atacgaatcc cggactcatc
      301 agacacaccg ccgccaccgt ataacggata ggccacaaca tctgataaac ggataccctc
      361 ccgccgcttc tcattgccgc acccgccaat aaaagaccgg acagccccgt atgcctgagc
      421 atcagcacac cggcggcaaa ggttgccgcc atcagaaaca gcgtccaacc gccgcccagc
      481 caatcggcaa cccacacaat cgacataatc tccaaaaaca gcagcaccaa aaaaccgata
      541 ccgaaaaatc tcattgaccg tcatccttat atttaagtaa acagcaaacc gcccgaacaa
      601 gactccaagc gacctgcctg taaatgatta caaaaccatg tgcttcaagc cgaaacaatg
      661 tgaaatcacg
//
LOCUS       DAWGJV010000256          661 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWGJV010000256 DAWGJV010000000
VERSION     DAWGJV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.256"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..73
                     /locus_tag="WHQ94_002055"
     CDS             <1..73
                     /locus_tag="WHQ94_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ2129274.1"
                     /translation="LQDWIDYYTFKTDKLVFGNAKRE"
     gene            115..303
                     /locus_tag="WHQ94_002056"
                     /pseudo
     CDS             115..303
                     /locus_tag="WHQ94_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            306..>661
                     /locus_tag="WHQ94_002057"
     CDS             306..>661
                     /locus_tag="WHQ94_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ2129275.1"
                     /translation="MGYNMMTTQKCHEHITDVIIGTTQRFNTEKSTDSIEYINERKGS
                     PIGAICIVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTPFFFPCD
                     MLNIQNPMFLMLMSDSP"
BASE COUNT          221 a          122 c          131 g          187 t
ORIGIN      
        1 tctgcaggat tggatagatt actatacgtt caaaaccgat aagctggtat ttggtaacgc
       61 gaagcgagag tgagccgtaa aactctgagc tcctgtttta tagattacaa ctttaggatg
      121 agtgaggatt ggattagaca agttattact aacttaccag ataatcatcc cactaaaaac
      181 atccttcgag aagcagtacg ttcgggtaaa atcaaaactg cggttactgg agtagatcgt
      241 caaacaggta aagcagttat tattcctgtt aaagttcctt ctaaagccaa tataaggaga
      301 taacaatggg atacaatatg atgaccaccc aaaaatgtca tgagcatatt acagatgtaa
      361 ttataggcac cactcaaaga tttaatacag agaaaagtac tgatagtata gagtatatta
      421 atgaaagaaa aggatctcca attggagcaa tctgtatcgt ttctatagat gtttatgcgg
      481 catgctcaca tgcttatctg tttgaacaca accttaaaaa gttcaaacag tatgcttatg
      541 cttacagtaa acttgagata ctggcaagta tgggatggtc tgaccccacg cctttcttct
      601 ttccctgtga catgctcaac attcaaaatc cgatgtttct gatgctgatg agcgacagcc
      661 c
//
LOCUS       DAWGJV010000257          619 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWGJV010000257 DAWGJV010000000
VERSION     DAWGJV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.257"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            335..619
                     /locus_tag="WHQ94_002058"
     CDS             335..619
                     /locus_tag="WHQ94_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2129276.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          184 a           97 c          159 g          179 t
ORIGIN      
        1 ttggacagcg gcaatattca aagattatct gaaagtccga gattctagat tcccactttc
       61 gtgggaatga cgggatttga gattgcggca tttatcggaa aaaacagaaa ccgctccgcc
      121 gtcattcccg cgtaggcggg aatctaggtt tgtcggtgcg gaaacttatc gggtaaaacg
      181 gtttctttag attttgcgtt ctagattccc actttcgtgg gaatgacgaa gagttgcggg
      241 aatgatggaa agctatggga ataacgaaag gttaaagtaa tcacgggatg gtgttcgcgg
      301 gaatataaat ttaaataatt caaaagggta ttatatgcag cctgcggttt atattttagc
      361 aagccaacgt aatggcacgt tatacattgg cgttacatct gatttggtgc aacgtattta
      421 ccaacatagg gaacatttga ttgagggatt tacatcacgg tacaacgtta ctatgctggt
      481 ttggtatgaa ctgcatccta cgatggagag cgcaattact cgggaaaaac agttgaagaa
      541 atggaacagg gcttggaaat tgcaactgat tgaagaaaat aatgtttctt ggcaggattt
      601 atggtttgat attatttag
//
LOCUS       DAWGJV010000258          614 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWGJV010000258 DAWGJV010000000
VERSION     DAWGJV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.258"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..346)
                     /locus_tag="WHQ94_002059"
     CDS             complement(<1..346)
                     /locus_tag="WHQ94_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129277.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRLFTVRKFLDFSELLEM
                     VNTQGYFLEFLYL"
     gene            444..>614
                     /locus_tag="WHQ94_002060"
     CDS             444..>614
                     /locus_tag="WHQ94_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ2129278.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLEQVIDWQPIEHYLNRQKTR
                     YLRDHRGRPAYPL"
BASE COUNT          201 a          152 c           97 g          164 t
ORIGIN      
        1 acaaatacag aaactcaaga aaataacctt gtgtattgac catctcaagc aattcagaaa
       61 aatcaagaaa ttttctgacc gtaaacaaac gtttccctaa aaaaacgatg tcttcaaaaa
      121 tatcgaacaa ataatcatct tcggcgtttt catttttatg ggttaaaaca acacgggaaa
      181 aatctgtttt cagatgcttg cccgcttgat tgttcggatt attgtccgga acgacaaaac
      241 cgtcctcaaa attaaagcag acgttgcgtc cttctacctt tatctctgtg caataacaat
      301 catgtagaga aatgctatcc gaaaaatttt ttctttgtgt atgcaaaaaa agttttcatt
      361 caagtaccca tatctaacac gagacctttg caaaattccc caaaatcccc taaattccca
      421 ccaagacatt taggggattt tccatgagca ccttcttccg gcaaaccgca caagccatga
      481 ttgccaaaca catcgaccgt ttcccactat tgaagttgga gcaggtgatt gattggcagc
      541 cgatcgaaca ctacctgaac cgtcaaaaaa cccgttacct tagagaccac cgcggccgtc
      601 ctgcctatcc cctg
//
LOCUS       DAWGJV010000259          584 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWGJV010000259 DAWGJV010000000
VERSION     DAWGJV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.259"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(97..318)
                     /locus_tag="WHQ94_002061"
     CDS             complement(97..318)
                     /locus_tag="WHQ94_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129279.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKEDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
BASE COUNT          203 a          113 c           68 g          200 t
ORIGIN      
        1 ctgataattt cctggttttt atcagcttta tcctcagatt ccaaacccaa taataaaagc
       61 cgtttgaaat ttttttcaga cggccttaaa acttaattaa ataatgtccg gatttaacac
      121 caaatcatat tcaccaaact ttggaaatag aattacctta tctttttggg aatataattc
      181 tgttttttca tatcgctcat tattaataaa atcctctttc aaaacatagc aatcatccgt
      241 attttcattg aagaaataag tatatccttc tgccaaatca tgagattcca aagttatata
      301 atctctccat acagccatag aaagaaaata ataattcgaa ttggaatatt tttgaaattg
      361 atgggttaaa taatcccttc caaatttatc gaaaaaatgt accgaaaaaa aacctacttc
      421 ctctcgcaag gtaattacat attcaggttg tgtcgccgaa cctattacgg cagtatataa
      481 ctccccttta agatgttttt cctcagcttg tttctcagaa aaaggcttat gaagtttctt
      541 ataaccataa gaccactgct cacaataaaa aatatctttc ttca
//
LOCUS       DAWGJV010000260          570 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWGJV010000260 DAWGJV010000000
VERSION     DAWGJV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.260"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..547)
                     /locus_tag="WHQ94_002062"
     CDS             complement(<1..547)
                     /locus_tag="WHQ94_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation factor family protein"
                     /protein_id="HEZ2129280.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSE"
BASE COUNT          168 a          146 c           86 g          170 t
ORIGIN      
        1 tttcagattt cggcctttga ttgctgttat caaaaaaacc attatcgtga tctaacaacg
       61 cctgatccgg cgtgtactct ccatcaaaaa aatcaagtgc taggtcaatt cgcgttattc
      121 ttgtccttat cgaatcatcg agaaactgct ttagcctcaa ctcccaaccc ggacttgcaa
      181 cgctgcaacc agtacctttc aactcaacta aaacagtatt gcgctgacct ccgaaatgca
      241 cctctccata atcaacatca tccgaaccta acctatacat ggattcatag aatttgttgc
      301 cccttgattt gcatttgcgc gttatgccaa aacctagaat ttcttccagc tttctgctta
      361 atacatacat gtattcagca tcagaaaata aagggcaacc ggaaactttc agtaaagtat
      421 cttcatggaa tgtgaatgaa atccaatcaa caaaaacccc gtcttgctta cctcttcgct
      481 gcggaatttc taaaagcttc ccttttccgt ccgatataaa gtgtgagaaa tattcaactt
      541 cactcattcg ttcactactc ttggaattgg
//
LOCUS       DAWGJV010000261          569 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWGJV010000261 DAWGJV010000000
VERSION     DAWGJV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.261"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(123..215)
                     /locus_tag="WHQ94_002063"
     tRNA            complement(123..215)
                     /locus_tag="WHQ94_002063"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Ser,seq:gct)
     gene            complement(372..569)
                     /locus_tag="WHQ94_002064"
     CDS             complement(372..569)
                     /locus_tag="WHQ94_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2129281.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
BASE COUNT          160 a          139 c          112 g          158 t
ORIGIN      
        1 tgttaatcca ctataacaaa attcagaatc aagacacggc aataccatac tggttttgca
       61 ccaacccgct ataaaattgc ataaaaaata gacgctgtaa aacacagcgt ctatttttta
      121 tctggcggag taagagggat tcgaaccctc gatgcaggtt gaccccacat gcttccttag
      181 cagggaagtg ccttcagcct ctcagccatt actcctaagg aagctgtaac tataatcaac
      241 ttttccgata ccgtcaagga aaaacagaaa gcaaatatta atcttttgaa ataaaataag
      301 atttaccctg attataccca atcaggcact tcttttcttc ctgaatggaa atattcctga
      361 ctgccacatc ctcaagcaaa atgcttttgt gcatcctgtt tgatttcctg aaaccacgcg
      421 tcttcaggaa aacgtgcaag cgcgacatcc aatgttgaca gcaggttttg cgacagggct
      481 ttggtcaggt attcctcctc gttggcttct gccgctttgt cgggctgttg tgccaaaccg
      541 tcggcacggg ctttgtgtcc gtgtgccaa
//
LOCUS       DAWGJV010000262          535 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWGJV010000262 DAWGJV010000000
VERSION     DAWGJV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.262"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
BASE COUNT          135 a          145 c          131 g          124 t
ORIGIN      
        1 gaatcgtcag gaatacccga atcgtcagga atacccgaac cgtcagaaat acccgaaccg
       61 tcattcccgc gcaggcggga atccagacct gtcggcacag aaacttatcg ggtataacgg
      121 tttcttgaga ttccgcattc tagattcccg cctgcgcggg aatgacggcg cataagtttc
      181 tgtgttgatt tgagtctatg ggaacggtgt aggattggtg gaatcgttgg aatcggtggg
      241 ctgaagcccg ccctataact caccttttac gaatcctcct acaccctata caacaccttg
      301 aaaatccacc ttgtcgggaa tgcccgaacc gtcagaaata cccgaatcgt cattcccgcg
      361 caggcgggaa tccagacctg tcggcacaga aacttatcgg gcaaaacggt ttcttgagat
      421 tccgcatcct agattcccgc ctgcgcggga atgacggctg cagatgccca acggtcttta
      481 ttcctccatc tctaagattt acagcgatac acgggtagtt taaggaatgt ccgaa
//
LOCUS       DAWGJV010000263          516 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWGJV010000263 DAWGJV010000000
VERSION     DAWGJV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.263"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
BASE COUNT          113 a          119 c          145 g          139 t
ORIGIN      
        1 ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcga
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacggtgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaagatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gcgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagaca
//
LOCUS       DAWGJV010000264          514 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M27306
            SAMN09011424-rid17424653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWGJV010000264 DAWGJV010000000
VERSION     DAWGJV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011424
            Sequence Read Archive: SRR7106899
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001660151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001660151.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:12:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,925
            CDSs (with protein)               :: 1,925
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 10 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011424-rid17424653.denovo.264"
                     /isolate="M27306"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            13..>514
                     /locus_tag="WHQ94_002065"
     CDS             13..>514
                     /locus_tag="WHQ94_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ2129282.1"
                     /translation="MQADLAYAAERITHDYPKATGANNTSTVSDYFRNIRTHSIHPRV
                     SVGYDFGGWRIAADYASYRKWKESNYSKKVTEFKHQNGTKKEDKTEHQGNGSFHAASS
                     LGLSAIYDFKLNDKFKPYIGARIAYGHVKHQIHSVESKTTTVTSNNGGPIPQGPTPKP
                     PYHESNS"
BASE COUNT          172 a          141 c           96 g          105 t
ORIGIN      
        1 agcccatatt atgtgcaggc ggatttagcc tacgccgccg aacgtattac ccacgattat
       61 ccgaaagcaa ccggtgcaaa caacacaagc acagtaagcg attatttcag aaacatccgt
      121 acgcattcca tccatccccg tgtgtcggtc ggctacgatt tcggcggctg gagaatagcg
      181 gcagattatg ccagttatag aaaatggaaa gaaagtaatt attctaaaaa agttactgaa
      241 tttaaacacc aaaacggcac caaaaaagaa gacaaaacag aacatcaagg aaacggcagc
      301 ttccacgccg cttcttctct cggcttatcc gccatttacg atttcaaact caacgataaa
      361 ttcaaaccct atatcggtgc gcgcatcgcc tacggacacg ttaaacatca gattcattca
      421 gtggaaagca aaaccacgac tgttacctct aacaacggag gtcctatccc acaaggtccg
      481 acccccaaac ctccctatca cgaaagcaac agca
//
